LOCUS       DAJTRB010000001       136723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTRB010000001 DAJTRB010000000
VERSION     DAJTRB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.001"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO330_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO330_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5881045.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO330_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO330_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5881046.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO330_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO330_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO330_000004"
                     /pseudo
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO330_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO330_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO330_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5881047.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO330_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO330_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5881048.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO330_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO330_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5881049.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO330_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO330_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5881050.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO330_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO330_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5881051.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO330_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO330_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5881052.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO330_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO330_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5881053.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO330_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO330_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5881054.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO330_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO330_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5881055.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO330_000014"
     CDS             18619..18894
                     /locus_tag="OO330_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881056.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO330_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO330_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5881057.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO330_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO330_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5881058.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20840..20911
                     /gene="ypdK"
                     /locus_tag="OO330_000017"
     CDS             20840..20911
                     /gene="ypdK"
                     /locus_tag="OO330_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5881059.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="OO330_000018"
     CDS             complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="OO330_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5881060.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22591..24077
                     /locus_tag="OO330_000019"
                     /pseudo
     CDS             22591..24077
                     /locus_tag="OO330_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24092..24826
                     /gene="ypdB"
                     /locus_tag="OO330_000020"
     CDS             24092..24826
                     /gene="ypdB"
                     /locus_tag="OO330_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5881061.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24839..25696
                     /locus_tag="OO330_000021"
     CDS             24839..25696
                     /locus_tag="OO330_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5881062.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25699..28194)
                     /gene="ptsP"
                     /locus_tag="OO330_000022"
     CDS             complement(25699..28194)
                     /gene="ptsP"
                     /locus_tag="OO330_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5881063.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28219..29256)
                     /gene="ypdE"
                     /locus_tag="OO330_000023"
     CDS             complement(28219..29256)
                     /gene="ypdE"
                     /locus_tag="OO330_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5881064.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29256..30339)
                     /gene="ypdF"
                     /locus_tag="OO330_000024"
                     /pseudo
     CDS             complement(29256..30339)
                     /gene="ypdF"
                     /locus_tag="OO330_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30354..31601)
                     /locus_tag="OO330_000025"
     CDS             complement(30354..31601)
                     /locus_tag="OO330_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5881065.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31623..31949)
                     /locus_tag="OO330_000026"
     CDS             complement(31623..31949)
                     /locus_tag="OO330_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5881066.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32168..33133)
                     /gene="glk"
                     /locus_tag="OO330_000027"
     CDS             complement(32168..33133)
                     /gene="glk"
                     /locus_tag="OO330_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5881067.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMVIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33337..34593
                     /locus_tag="OO330_000028"
     CDS             33337..34593
                     /locus_tag="OO330_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5881068.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34708..35034
                     /locus_tag="OO330_000029"
     CDS             34708..35034
                     /locus_tag="OO330_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5881069.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35175..36413)
                     /locus_tag="OO330_000030"
     CDS             complement(35175..36413)
                     /locus_tag="OO330_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5881070.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36749..37951
                     /gene="nupC"
                     /locus_tag="OO330_000031"
     CDS             36749..37951
                     /gene="nupC"
                     /locus_tag="OO330_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5881071.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38001..39316)
                     /locus_tag="OO330_000032"
                     /pseudo
     CDS             complement(38001..39316)
                     /locus_tag="OO330_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(39456..40082)
                     /locus_tag="OO330_000033"
     CDS             complement(39456..40082)
                     /locus_tag="OO330_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR5881072.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRIYLKLAGSVKRAATSVVHTL"
     gene            complement(40290..40365)
                     /locus_tag="OO330_000034"
     tRNA            complement(40290..40365)
                     /locus_tag="OO330_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40330..40332),aa:Ala,seq:ggc)
     gene            complement(40405..40480)
                     /locus_tag="OO330_000035"
     tRNA            complement(40405..40480)
                     /locus_tag="OO330_000035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40445..40447),aa:Ala,seq:ggc)
     gene            40701..41060
                     /locus_tag="OO330_000036"
     CDS             40701..41060
                     /locus_tag="OO330_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5881073.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41062..41454
                     /locus_tag="OO330_000037"
     CDS             41062..41454
                     /locus_tag="OO330_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5881074.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41506..42921)
                     /gene="gltX"
                     /locus_tag="OO330_000038"
     CDS             complement(41506..42921)
                     /gene="gltX"
                     /locus_tag="OO330_000038"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5881075.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGAFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43180..43255
                     /locus_tag="OO330_000039"
     tRNA            43180..43255
                     /locus_tag="OO330_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43213..43215,aa:Val,seq:tac)
     gene            43300..43375
                     /locus_tag="OO330_000040"
     tRNA            43300..43375
                     /locus_tag="OO330_000040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43333..43335,aa:Val,seq:tac)
     gene            43380..43455
                     /locus_tag="OO330_000041"
     tRNA            43380..43455
                     /locus_tag="OO330_000041"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43413..43415,aa:Lys,seq:ttt)
     gene            43486..43611
                     /locus_tag="OO330_000042"
     ncRNA           43486..43611
                     /ncRNA_class="other"
                     /locus_tag="OO330_000042"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43731..44072
                     /locus_tag="OO330_000043"
     CDS             43731..44072
                     /locus_tag="OO330_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5881076.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44063..44989)
                     /locus_tag="OO330_000044"
     CDS             complement(44063..44989)
                     /locus_tag="OO330_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5881077.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45079..46077
                     /locus_tag="OO330_000045"
     CDS             45079..46077
                     /locus_tag="OO330_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5881078.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46074..46292)
                     /locus_tag="OO330_000046"
     CDS             complement(46074..46292)
                     /locus_tag="OO330_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5881079.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46294..48309)
                     /gene="ligA"
                     /locus_tag="OO330_000047"
     CDS             complement(46294..48309)
                     /gene="ligA"
                     /locus_tag="OO330_000047"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5881080.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFVGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48380..49366)
                     /gene="zipA"
                     /locus_tag="OO330_000048"
     CDS             complement(48380..49366)
                     /gene="zipA"
                     /locus_tag="OO330_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5881081.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49596..50357
                     /gene="cysZ"
                     /locus_tag="OO330_000049"
     CDS             49596..50357
                     /gene="cysZ"
                     /locus_tag="OO330_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5881082.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPAFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50542..51513
                     /gene="cysK"
                     /locus_tag="OO330_000050"
     CDS             50542..51513
                     /gene="cysK"
                     /locus_tag="OO330_000050"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5881083.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51897..52154
                     /gene="ptsH"
                     /locus_tag="OO330_000051"
     CDS             51897..52154
                     /gene="ptsH"
                     /locus_tag="OO330_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5881084.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52199..53926
                     /gene="ptsI"
                     /locus_tag="OO330_000052"
     CDS             52199..53926
                     /gene="ptsI"
                     /locus_tag="OO330_000052"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5881085.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53967..54476
                     /gene="crr"
                     /locus_tag="OO330_000053"
     CDS             53967..54476
                     /gene="crr"
                     /locus_tag="OO330_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5881086.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54518..55369)
                     /gene="pdxK"
                     /locus_tag="OO330_000054"
                     /gene_synonym="thiJ"
     CDS             complement(54518..55369)
                     /gene="pdxK"
                     /locus_tag="OO330_000054"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5881087.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55474..55848
                     /locus_tag="OO330_000055"
     CDS             55474..55848
                     /locus_tag="OO330_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5881088.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55881..56604
                     /locus_tag="OO330_000056"
                     /pseudo
     CDS             55881..56604
                     /locus_tag="OO330_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56808..57719)
                     /gene="cysM"
                     /locus_tag="OO330_000057"
     CDS             complement(56808..57719)
                     /gene="cysM"
                     /locus_tag="OO330_000057"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5881089.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSLGAGI"
     gene            complement(57853..58950)
                     /gene="cysA"
                     /locus_tag="OO330_000058"
     CDS             complement(57853..58950)
                     /gene="cysA"
                     /locus_tag="OO330_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5881090.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58940..59815)
                     /gene="cysW"
                     /locus_tag="OO330_000059"
     CDS             complement(58940..59815)
                     /gene="cysW"
                     /locus_tag="OO330_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5881091.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59815..60648)
                     /gene="cysT"
                     /locus_tag="OO330_000060"
     CDS             complement(59815..60648)
                     /gene="cysT"
                     /locus_tag="OO330_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5881092.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVTLSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60648..61664)
                     /gene="cysP"
                     /locus_tag="OO330_000061"
     CDS             complement(60648..61664)
                     /gene="cysP"
                     /locus_tag="OO330_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5881093.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61968..62759)
                     /gene="ucpA"
                     /locus_tag="OO330_000062"
     CDS             complement(61968..62759)
                     /gene="ucpA"
                     /locus_tag="OO330_000062"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5881094.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62946..63746)
                     /gene="murR"
                     /locus_tag="OO330_000063"
     CDS             complement(62946..63746)
                     /gene="murR"
                     /locus_tag="OO330_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5881095.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63910..64806
                     /gene="murQ"
                     /locus_tag="OO330_000064"
     CDS             63910..64806
                     /gene="murQ"
                     /locus_tag="OO330_000064"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5881096.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64810..66234
                     /gene="murP"
                     /locus_tag="OO330_000065"
                     /pseudo
     CDS             64810..66234
                     /gene="murP"
                     /locus_tag="OO330_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(66414..67313)
                     /gene="yfeX"
                     /locus_tag="OO330_000066"
     CDS             complement(66414..67313)
                     /gene="yfeX"
                     /locus_tag="OO330_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5881097.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67409..67984)
                     /locus_tag="OO330_000067"
     CDS             complement(67409..67984)
                     /locus_tag="OO330_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5881098.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68045..68494)
                     /locus_tag="OO330_000068"
     CDS             complement(68045..68494)
                     /locus_tag="OO330_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5881099.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68481..68906)
                     /locus_tag="OO330_000069"
     CDS             complement(68481..68906)
                     /locus_tag="OO330_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5881100.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69120..69989
                     /gene="amiA"
                     /locus_tag="OO330_000070"
     CDS             69120..69989
                     /gene="amiA"
                     /locus_tag="OO330_000070"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5881101.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69993..70892
                     /gene="hemF"
                     /locus_tag="OO330_000071"
     CDS             69993..70892
                     /gene="hemF"
                     /locus_tag="OO330_000071"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5881102.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70898..71950)
                     /gene="eutR"
                     /locus_tag="OO330_000072"
                     /pseudo
     CDS             complement(70898..71950)
                     /gene="eutR"
                     /locus_tag="OO330_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71996..72496)
                     /gene="eutK"
                     /locus_tag="OO330_000073"
     CDS             complement(71996..72496)
                     /gene="eutK"
                     /locus_tag="OO330_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5881103.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72509..73168)
                     /gene="eutL"
                     /locus_tag="OO330_000074"
     CDS             complement(72509..73168)
                     /gene="eutL"
                     /locus_tag="OO330_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5881104.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74350..75141
                     /locus_tag="OO330_000075"
     CDS             74350..75141
                     /locus_tag="OO330_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881105.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75860..76255
                     /locus_tag="OO330_000076"
     CDS             75860..76255
                     /locus_tag="OO330_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5881106.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76273..76536
                     /locus_tag="OO330_000077"
     CDS             76273..76536
                     /locus_tag="OO330_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881107.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76559..77101
                     /locus_tag="OO330_000078"
     CDS             76559..77101
                     /locus_tag="OO330_000078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881108.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77240..77527
                     /locus_tag="OO330_000079"
     CDS             77240..77527
                     /locus_tag="OO330_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881109.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77566..77772
                     /locus_tag="OO330_000080"
     CDS             77566..77772
                     /locus_tag="OO330_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881110.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78084..78569
                     /locus_tag="OO330_000081"
     CDS             78084..78569
                     /locus_tag="OO330_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5881111.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78550..78828
                     /locus_tag="OO330_000082"
     CDS             78550..78828
                     /locus_tag="OO330_000082"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881112.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78821..80281
                     /locus_tag="OO330_000083"
     CDS             78821..80281
                     /locus_tag="OO330_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5881113.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80315..81124
                     /gene="murI"
                     /locus_tag="OO330_000084"
     CDS             80315..81124
                     /gene="murI"
                     /locus_tag="OO330_000084"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5881114.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81243..81937)
                     /locus_tag="OO330_000085"
     CDS             complement(join(81243..81691,81691..81937))
                     /locus_tag="OO330_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5881115.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82456..83829)
                     /gene="eutA"
                     /locus_tag="OO330_000086"
     CDS             complement(82456..83829)
                     /gene="eutA"
                     /locus_tag="OO330_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5881116.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83804..84283)
                     /gene="eutP"
                     /locus_tag="OO330_000087"
     CDS             complement(83804..84283)
                     /gene="eutP"
                     /locus_tag="OO330_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5881117.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84296..84631)
                     /gene="eutS"
                     /locus_tag="OO330_000088"
     CDS             complement(84296..84631)
                     /gene="eutS"
                     /locus_tag="OO330_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5881118.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84924..87203)
                     /gene="maeB"
                     /locus_tag="OO330_000089"
     CDS             complement(84924..87203)
                     /gene="maeB"
                     /locus_tag="OO330_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5881119.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87492..88428
                     /gene="tal"
                     /locus_tag="OO330_000090"
                     /pseudo
     CDS             87492..88428
                     /gene="tal"
                     /locus_tag="OO330_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
     gene            88448..90451
                     /gene="tkt"
                     /locus_tag="OO330_000091"
     CDS             88448..90451
                     /gene="tkt"
                     /locus_tag="OO330_000091"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5881120.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90554..91597)
                     /locus_tag="OO330_000092"
     CDS             complement(90554..91597)
                     /locus_tag="OO330_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5881121.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91723..92298)
                     /gene="nudK"
                     /locus_tag="OO330_000093"
     CDS             complement(91723..92298)
                     /gene="nudK"
                     /locus_tag="OO330_000093"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5881122.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92366..94345)
                     /gene="aegA"
                     /locus_tag="OO330_000094"
     CDS             complement(92366..94345)
                     /gene="aegA"
                     /locus_tag="OO330_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5881123.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94551..96268
                     /gene="narQ"
                     /locus_tag="OO330_000095"
                     /pseudo
     CDS             94551..96268
                     /gene="narQ"
                     /locus_tag="OO330_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96470..99583
                     /gene="acrD"
                     /locus_tag="OO330_000096"
     CDS             96470..99583
                     /gene="acrD"
                     /locus_tag="OO330_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5881124.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99682..99741)
                     /gene="ypfM"
                     /locus_tag="OO330_000097"
     CDS             complement(99682..99741)
                     /gene="ypfM"
                     /locus_tag="OO330_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5881125.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100122..100478
                     /locus_tag="OO330_000098"
     CDS             100122..100478
                     /locus_tag="OO330_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5881126.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100482..101609
                     /gene="dapE"
                     /locus_tag="OO330_000099"
     CDS             100482..101609
                     /gene="dapE"
                     /locus_tag="OO330_000099"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5881127.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101637..101837
                     /locus_tag="OO330_000100"
     CDS             101637..101837
                     /locus_tag="OO330_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5881128.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101947..102645)
                     /gene="ypfH"
                     /locus_tag="OO330_000101"
     CDS             complement(101947..102645)
                     /gene="ypfH"
                     /locus_tag="OO330_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5881129.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102719..104734)
                     /gene="tmcA"
                     /locus_tag="OO330_000102"
     CDS             complement(102719..104734)
                     /gene="tmcA"
                     /locus_tag="OO330_000102"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5881130.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104749..105612)
                     /locus_tag="OO330_000103"
     CDS             complement(104749..105612)
                     /locus_tag="OO330_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5881131.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105780..106493)
                     /gene="purC"
                     /locus_tag="OO330_000104"
     CDS             complement(105780..106493)
                     /gene="purC"
                     /locus_tag="OO330_000104"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5881132.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106706..107740)
                     /gene="bamC"
                     /locus_tag="OO330_000105"
     CDS             complement(106706..107740)
                     /gene="bamC"
                     /locus_tag="OO330_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5881133.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107757..108635)
                     /gene="dapA"
                     /locus_tag="OO330_000106"
     CDS             complement(107757..108635)
                     /gene="dapA"
                     /locus_tag="OO330_000106"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5881134.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108781..109353
                     /locus_tag="OO330_000107"
     CDS             108781..109353
                     /locus_tag="OO330_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5881135.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109353..109823
                     /gene="bcp"
                     /locus_tag="OO330_000108"
     CDS             109353..109823
                     /gene="bcp"
                     /locus_tag="OO330_000108"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5881136.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110076..110693
                     /gene="hyfA"
                     /locus_tag="OO330_000109"
     CDS             110076..110693
                     /gene="hyfA"
                     /locus_tag="OO330_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5881137.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110693..112710
                     /gene="hyfB"
                     /locus_tag="OO330_000110"
                     /pseudo
     CDS             110693..112710
                     /gene="hyfB"
                     /locus_tag="OO330_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112721..113668
                     /gene="hyfC"
                     /locus_tag="OO330_000111"
     CDS             112721..113668
                     /gene="hyfC"
                     /locus_tag="OO330_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5881138.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113685..115124
                     /gene="hyfD"
                     /locus_tag="OO330_000112"
     CDS             113685..115124
                     /gene="hyfD"
                     /locus_tag="OO330_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5881139.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115136..115786
                     /gene="hyfE"
                     /locus_tag="OO330_000113"
     CDS             115136..115786
                     /gene="hyfE"
                     /locus_tag="OO330_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5881140.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115791..117377
                     /gene="hyfF"
                     /locus_tag="OO330_000114"
     CDS             115791..117377
                     /gene="hyfF"
                     /locus_tag="OO330_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5881141.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAISWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            117367..119081
                     /gene="hyfG"
                     /locus_tag="OO330_000115"
                     /pseudo
     CDS             117367..119081
                     /gene="hyfG"
                     /locus_tag="OO330_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119091..119636
                     /gene="hyfH"
                     /locus_tag="OO330_000116"
     CDS             119091..119636
                     /gene="hyfH"
                     /locus_tag="OO330_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5881142.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119633..120391
                     /gene="hyfI"
                     /locus_tag="OO330_000117"
     CDS             119633..120391
                     /gene="hyfI"
                     /locus_tag="OO330_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5881143.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDLRLNSIVQQLFRVLRGLHD"
     gene            120384..120797
                     /gene="hyfJ"
                     /locus_tag="OO330_000118"
     CDS             120384..120797
                     /gene="hyfJ"
                     /locus_tag="OO330_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5881144.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120827..122839
                     /gene="hyfR"
                     /locus_tag="OO330_000119"
     CDS             120827..122839
                     /gene="hyfR"
                     /locus_tag="OO330_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5881145.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SKQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            122861..123709
                     /locus_tag="OO330_000120"
                     /pseudo
     CDS             122861..123709
                     /locus_tag="OO330_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123747..124808)
                     /locus_tag="OO330_000121"
     CDS             complement(123747..124808)
                     /locus_tag="OO330_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5881146.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            125021..126484
                     /gene="bepA"
                     /locus_tag="OO330_000122"
     CDS             125021..126484
                     /gene="bepA"
                     /locus_tag="OO330_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5881147.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            126505..126864
                     /gene="arsC"
                     /locus_tag="OO330_000123"
     CDS             126505..126864
                     /gene="arsC"
                     /locus_tag="OO330_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5881148.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(126997..127743)
                     /gene="hda"
                     /locus_tag="OO330_000124"
     CDS             complement(126997..127743)
                     /gene="hda"
                     /locus_tag="OO330_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5881149.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127793..129082)
                     /gene="uraA"
                     /locus_tag="OO330_000125"
     CDS             complement(127793..129082)
                     /gene="uraA"
                     /locus_tag="OO330_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5881150.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(129168..129794)
                     /gene="upp"
                     /locus_tag="OO330_000126"
     CDS             complement(129168..129794)
                     /gene="upp"
                     /locus_tag="OO330_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5881151.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            130119..131156
                     /gene="purM"
                     /locus_tag="OO330_000127"
     CDS             130119..131156
                     /gene="purM"
                     /locus_tag="OO330_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5881152.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            131156..131794
                     /gene="purN"
                     /locus_tag="OO330_000128"
     CDS             131156..131794
                     /gene="purN"
                     /locus_tag="OO330_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5881153.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREASDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            131966..134032
                     /gene="ppk1"
                     /locus_tag="OO330_000129"
     CDS             131966..134032
                     /gene="ppk1"
                     /locus_tag="OO330_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5881154.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            134037..135578
                     /gene="ppx"
                     /locus_tag="OO330_000130"
     CDS             134037..135578
                     /gene="ppx"
                     /locus_tag="OO330_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5881155.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135617..>136723)
                     /gene="pdeF"
                     /locus_tag="OO330_000131"
     CDS             complement(135617..>136723)
                     /gene="pdeF"
                     /locus_tag="OO330_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5881156.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        34136 a        35172 c        33600 g        33815 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgta ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctagtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taatcccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacagcc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac atcatgattt ttatagcttt ttcttaagtt ttttccttcg ctgaatatct
    20581 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    20641 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    20701 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct tttaaggtcg
    20761 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    20821 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
    20881 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    20941 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    21001 ttgctgctga cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    21061 tgacgaatac ggtcgtggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    21121 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    21181 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    21241 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    21301 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    21361 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    21421 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
    21481 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    21541 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    21601 acatcgtagc atttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    21661 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    21721 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    21781 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    21841 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    21901 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    21961 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    22021 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    22081 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    22141 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    22201 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    22261 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    22321 aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga aatttttcta
    22381 cgctaacaag aaaatcacca ccaattttat taacattata atttcaataa atttaccagc
    22441 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    22501 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    22561 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    22621 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    22681 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    22741 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    22801 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    22861 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    22921 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    22981 aaaatcccca aagcacagcg ctggcgctgg atatcgccaa caaaacgcta ccgctgtttc
    23041 gccatgtcaa tagcgagtca ttacgcaagg tctgcgaaat tatccgcgat gacattcacg
    23101 ccgatgcggt ggcgattact aataccgatc atgtgctggc ctatgttggc gtgggtgaac
    23161 ataactatca gaatggcgat gacttcatta gcccgactac ccgtcaggcg atgaattacg
    23221 gaaaaatcat cattaaaaac aatgatgaag cccaccgcac accagagatt cattccatgc
    23281 tggtgatccc attgtgggag aaaggggtcg tgaccggaac gctgaaaatt tactactgcc
    23341 acgcgcatca gatcacctcg tcattacagg aaatggcggt cggtctgtcg caaatcatct
    23401 ccacgcaact ggaggtttca cgcgccgagc agctacgtga aatggcaaat aaggcagagc
    23461 ttcgcgccct gcaaagcaaa attaatcccc attttctgtt taacgctctg aacgctattt
    23521 catcgtcaat ccgtctgaat ccggataccg ctcgccagtt gatctttaat ctgtcgcgtt
    23581 atctgcgcta taacattgaa ttaaaagacg atgagcaaat cgatatcaaa aaagagctgt
    23641 atcaaattaa agactatatt gccattgagc aggcccgctt tggcgacaag ctgacggtta
    23701 tctatgatat tgatgaagag gtgaattgct gcattcccag cctgctgatc cagccgttgg
    23761 tggagaacgc cattgtccac ggtattcagc cttgcaaagg taaaggcgtt gtcaccatca
    23821 gcgttgcaga gtgcggaaat cgggtacgca ttgcggtgcg agataccggg cacggcatcg
    23881 ttccaaaggt gattgagcgg gtcgaagcga atgaaatgcc gggcaataaa attggcctgc
    23941 tgaatgtcca tcatcgcgtg aagttattgt atggcgaggg gctgcatatc cgccgcctgg
    24001 agccggggac ggaaattgcg ttttacattc ctaaccaacg caccccagtc gcctcacagg
    24061 ctacgttatt gctttgagcc ggagtgatat tgtgaaagtc atcattgttg aagacgaatt
    24121 cctggcacaa caggaactga gctggctaat taaagagcac agccagatgg agattgtcgg
    24181 cacctttgac gacggtctgg acgtgttgaa gtttttgcag cataaccgcg tcgacgccat
    24241 ttttctggat atcaatattc cgtcgctgga tggcgtgttg ctggcgcaaa acatcagcca
    24301 gttcgcccat aaaccgttta ttgtgttcat caccgcgtgg aaagaacatg cggtagaagc
    24361 gtttgaactg gaggcgtttg actacattct caaaccgtat caggagtcac gtattaccgg
    24421 gatgctgcaa aaactggaag cggcctggca acaacagcag accagcagta cgcctgccgc
    24481 gacggtaacg cgtgagaatg acaccattaa tctggtgaaa gatgagcgaa taatcgtcac
    24541 gccaattaac gatatctatt acgccgaagc gcacgagaaa atgacctttg tctatacgcg
    24601 gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt tgcagcaaac tgccgccgtc
    24661 gcattttttc cgctgccatc gctcattttg tgtcaatctg aacaaaatac gcgaaatcga
    24721 accgtggttt aataacacct acattctgcg actgaaagat ctggattttg aagtgccggt
    24781 cagccgcagc aaagtgaaag aatttcgcca gttaatgcat ctttaatcgg gaactttcat
    24841 gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc gatctgttca gcggcctagt
    24901 gcttaacccg caactactgg ggcgcgtctg gtttgccagc cagcctgcct cgttaccggt
    24961 gggcagttta tgtattgatt ttccccgtct ggatatcgtg ctgcgcggcg aatacggcaa
    25021 cctgctggaa gcaaagcagc agcgaatggt ggaaggagaa atgctgttta ttccggcacg
    25081 cgcggctaat ttaccgatca acaacaaacc ggtgatgctg ttaagcctgg tgttcgcacc
    25141 gacctggctt gggttatcgt tttacgatag ccgcaccacg tcgttgttgc atcctgctcg
    25201 ccagacccag cttcccagcc tgcaacgcgg tgaaggtgaa gcgatgctta ccgccctcac
    25261 ccatcttagc cgttcgccgc tggagcagaa tatcattcag ccactggtgt taagtttgct
    25321 gcatctttgc cgcaacgtgg tgaatatgcc gccgggtaat tcacaaccgc gcggcgattt
    25381 tctctatcac agcatttgta actgggttca ggataattat gcccagccgc tcacccgcga
    25441 gagcgtggcg cagtttttta atatcacgcc caatcatctg tcaaaactgt ttgctcagca
    25501 tggaacgatg cgttttatcg agtatgtgcg ttgggtgcga atggcgaagg cgaggatgat
    25561 tttgcagaaa tatcatctgt caattcatga agtggcacag cgttgcggtt ttccggatag
    25621 cgactatttt tgtcgcgttt tccggcgtca gtttagtctg acgccgggag agtacagcgc
    25681 ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc agcagcgtca ggatactttg
    25741 tgcatcttgc gcggcaaaca acgactggcg gaagttttta ttcaccagtt tacgcgccag
    25801 ctgcgagaag actttcacat gattcatccc ttcgttagca cccagcgtca gcatgatcac
    25861 cagttcgact tcgcccattt ctgactgcca gtcaaccggt ttcgccagcc gggcaatgct
    25921 gatactggaa tgacggatcc actgagattt ggtgtgcggg atcgctacgc caaaaccaac
    25981 gccggtggta acaatctctt cccgctgcca gacatcttct tccagctcaa acggatgttc
    26041 agtgcgcccg ttaacgccga ggttgccgca caggaactgg atcgcctgct ctttattgct
    26101 aaaatcctga tcaacaaaga tattctccag cgccagcagt gggcgaacgt cttcttccgg
    26161 cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcactg cggcattcac atgcctgacg
    26221 cgccagttcc cgacacgcct cgctatccag ttgacgaagc tggcttttca ccgccggaat
    26281 acgcgggcta ctcatactca gctcatccag gcccagccca agcagtagcg gcagataacg
    26341 gctttcaccg cccagttcac cgcaaatgcc tacccatttg ccccgctgat gcgcagtggt
    26401 aactatttgc tgcaacatgc gcaggaacga tggcgtaatc gggttatata gcggcgatac
    26461 gcgcgggtta ttacgatcga ccgcatacag atactgggtc atatcgttgg agccgatact
    26521 gaagaaatcg acctcatcgc agaagtggtc gatgatgtag cacaccgacg gcacttccac
    26581 catgatccca agcgtaatcg tctctgcatg acgcaggcca tcgcgcttaa gctcaacgat
    26641 cgctttttgg atctcgcctt tcacccataa gatctgatcg aggctgtgaa ccatcgggat
    26701 catcaactgg gcgttgccga aactggcggc gcgcaaaatg gcccgcagtt gagtgcggaa
    26761 caggccagca aattccgggt aaatacgtac cgcgcgatag ccgaggaacg ggttctcttc
    26821 ctggggaata ttcagataag gaatgctttt atcgccgccg atgtccatcg tgcggaagat
    26881 aatcggcttg tcgcccgccg ccagtagcac ctgctggtag gcttcaaatt gctcctgctc
    26941 gtccggcgcg ctgtcgcgat ccatatagag catttcggta cggaacagcc cgacaccttc
    27001 cgcgccgttg gcaaacgcgc ctggcgcttc cagagcggtg ccgatattcg ccgcaatatc
    27061 aatacgcttg ttatcacggg aataggccag ctgcgcggca gcctgcgcct gttgtttttg
    27121 gcgtttatcc gccagcgtct gcgcgacctg ataataaccg ctcaccgcgt cattcgggtt
    27181 aatcgccagc acgccgcact gggcgtcaag cactgcaggt tgcccggcat aacgggcaat
    27241 cgcatccagc ggcaagccac tcagtaccgg gatcgccgag gcacgggcca gaatcagtgt
    27301 atgcgaggtg cgcccggttt tctccagaat catacccgca agatttttca aatcaaggct
    27361 caaaaactgg cttggggtta aatcttcagc caccagaatg gtcggttttt ccagcaccag
    27421 attgttgcgc ggcttcagtt ccggccaggt gatatgcagc aactgttcgc tgatatcgcg
    27481 aatgtcgctg acccgctcgc gcagataatc gctggcagaa gcagatagtt tggcgcaaac
    27541 ctgctccata ttgctgatga tcgccgcccc cagtccctga tgctgttctg tcatcaggcg
    27601 acggatattg cctgcaaatt catcatcctg aatcagcgac aaatgagcgc tgaggatagt
    27661 tttgctttcg ccgtcacgct cacgcaattg ctgattcagt tgctcggcaa gcgttgccag
    27721 gctgtgctcc agccgggtgg aatcttgcgc actggcgggg attgcccgat aactgtcgag
    27781 gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg ccgcttgcca gcacattgcc
    27841 gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc agcggatgcg ccgtcagttc
    27901 tgcctgcgtc ggctgaacgc tgtcgctgtc gataaagcgc accaggatgt actcttccag
    27961 cacgcgccgc gcctgctctt catcgctgcc gctaatgttc aggctgcaac tgtcattaaa
    28021 taaggtgcca gttccaatca acgccagcga gcttttagca tcagcctttg cgttctggcg
    28081 atggttgata aaggtgattt cgctttgcca ctggctgcac tgttctttaa gttcccacgc
    28141 cggacgagca tgtagaccgt taggcaaagg acagagaaat tgaatcgtta acataaccgc
    28201 tccgggttaa tcaggagatc atctgaaatc cgtcagttga acaaccgtct cacgcgtaag
    28261 acgttgaata agggcagata aaagttgctc catctgcaaa atgtcgcggc aatcggcaat
    28321 cgatgccgcg caatgtccgt ggcgggtggc aggccccatt accaatgtgg gtacgccagt
    28381 accggttaag tgcaccgctc caccgtccgt gccgccgtta ctgaacatat ccgcctgcaa
    28441 cggaacgcca atttctgccg ccacggtttc aatccacgcg gtaagttttg gcggcgcaat
    28501 cagcgattta tcgcttaaca ccagcatcgg cccgttacca atctgacgat ggttagccgc
    28561 gccataatca aagtttttcg cccagcaggc ggtatcaagc acaatggcga cgtccggcga
    28621 caccgcgcgg gtagcagttt gcccgccgcg taatcccacc tcttcgctgg aacttgccac
    28681 cagccacact tccgcaggca gttcggcgtc gtgcagctca cgtagtaacg tcaccagcag
    28741 atagcaactg aggcgatcat caaaggcttt gcccatcacc cgctggtgag ggaggacctg
    28801 aaaagtggta tcgaaggtga cgcgatcgcc tggacgaatt cccgcctgca tcacttcgtc
    28861 ataggtgcgc gcgccaatgt ccacgcgcat ggcgctaacg tcattcccct gccggtcgcc
    28921 gtcaagcagg cctggaattt tgcactcttc acgggtggtg atgcgcaccg gctgcagctg
    28981 gcgggcagcc atgcgcacgt tgccaaccgg cagcacgtca atagcgcctt cgcgggagat
    29041 gctgcgcacc ataaatccca cttcgtccat atgggcgcag atcatcacct tcggacctgt
    29101 cgattcattg aggcggatca gcaccgatcc cagaccatca aatcgcactt ctttttgcag
    29161 gcgagccgct tcttccagca ggatctgccg cacttcctgt tccgaggagg cgatcgcatc
    29221 tgcctcgctc aacgctttta atagcgataa atccattatg cctctcccgt gagcaacact
    29281 gttttcggca tggcgtagag cacttctgcg ccttgcgggg taaccagcac aacatcttcg
    29341 atgcgcacgc ccccttgccc tagcaaataa atccccggct ccacggtcag caacatgcct
    29401 ggctgtagcg tcgtggtgtc ccgcggtgaa aaacgcggat cttcatggac ttcaatgccg
    29461 atagcgtgac cagtgttatg accgaaatag tcgccaaatc cagcctcggt aatgacccgg
    29521 cgcgcggcgt cgtcaatctg ctggcagcgc acgccggggc gaattgcaga gattgccgcg
    29581 agctgtgcct gcaggacaat ctgatagacg tcaaacagcg ggtgagattc ggcgctcacc
    29641 ccttcgccat tcaccagcaa ggtgcgcgtc atatcagcag tagccctgat acagcgcacc
    29701 aaaatcgaga gtgacaaact cgcccgctgc aacaatcttg tcgctggctt tgccgtgcgg
    29761 cagcgccccg cgccagccgc tggcgacaat ggtgtcaaaa gaggcttttt ctgcgccctg
    29821 ctggcgcata aaccactcca gttcagcggc tatctcgcgc tcgctcatcc ccgcctgaat
    29881 aaagcggcga atatgctccg ctccgcgatc ggcaatccca caggcgaggc ggattatctc
    29941 cacctcctct ggcgttttga tttgccgcag cacatccggc gtggcgctaa ccagtttcgc
    30001 attgagttca gacttccagc ggtgcgcggt ttcccaactc acctgctggc cttcaaaacc
    30061 gagcgtttgc aactgctcat cggcaatgat ttgattgacg atagtggtaa gcgtgttcgt
    30121 cgcgtcaagc aaatgcagct ggtagccttg cgcacgggct tccacctcaa cgaaatagcg
    30181 cgaatctacc agaatgtgcg cactttcacg gctaataacc acatagcctg atccggtgga
    30241 gatccccaga tgcggctgtt tgttctgccg tgaggagaga agcaccgcat ccagttgttg
    30301 caccttaagc cagttgcgca gcgaagcgag taatgtcatc tggattttcc ttcttacagg
    30361 ctatcgatta acaatttgcc tttgcggaac atcatcagac gcaggaacac caccatcagc
    30421 gcagtaatga ttgcccccag cgcgatcccc gccatataaa cgcccaggtt agtgaccagc
    30481 ggccatgccc agatagcgga ttccgggaac cattgcactg cgcccagcca gacagctgcg
    30541 gttgagccga caatcgcgcc gaccatatat gacggaatag cggtgatggg gctttccagc
    30601 gcaaacggaa tcgcaccctc actaatcccc ataaaggcca ggaacatcgc ggtcttaccc
    30661 tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta aacgacggtc aataatggtc
    30721 gccagaccca gaccaatcgg cggaataacg atagcgatag aacgagcggt gaccggtaat
    30781 acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg ctttgttgat cgggccacca
    30841 aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca tcagcgcgcc cttctcacct
    30901 gcggcagtca gcacagtacg gataccgccg ttgatccagc caccaaaggg agtgatgacg
    30961 tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg gtagcaaaaa tgtggttttg
    31021 aacgctaaca ggaaatccgg taactggatt ttctggttca tccatttcac aaggtaaccg
    31081 gcaacgatag aaataatcag cgcaccaatg aaggtggacg gcaccggcga agaggtcgcc
    31141 cattgcatag tgctgggatc gaagttcagc agttgcgtcg gctgggtaga catcaacccg
    31201 ccgataaaac cagccgggaa cgccagtttg ccaccgatag agttcgccac aaatgcagca
    31261 aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg actgcgacag ccaggcaaat
    31321 ttaagtaagg agaggtcgaa accggagaat ttcccgctat taagagcatc catgatgccg
    31381 atctcagcag gaattttaag ccagctataa gcaatcagct ggctgaaagc gaggatcaca
    31441 ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg acatcacatg ctgcgggagt
    31501 tcaccccaga agctgctttt agaggcctgc ggattcttaa ctgccgctgc cgcaccggat
    31561 gcgccaggca caacggttgc actgcgtttt ttaatggcca taacaattat ccctgtcgaa
    31621 tattactgtt gttcagaggc aatcatctct tcgatttctt tgatgatgcc cgcagcgttt
    31681 ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg attcgaaacg ttcgttatct
    31741 tccggggtaa ctgccacgga atggatgatg atggtcgctt cggcgatatc ctgcgccgtc
    31801 aggcgattct ggatgccgtc cgcgccctgg gtttcaattt tcacttcata accggcttct
    31861 accgccgctt cttccagcac ctgagcggcc ataaaagtgt gagctaggcc cattgggcag
    31921 gcacataagg caatcagttt cttactcatc gttaattcct cgttcagtga tagttgacga
    31981 gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga caaataatgc atttactgga
    32041 gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc ttgtgagggg acacaaagaa
    32101 aaattgaatt aactgtatga tttaaaagat tatcgggaga gttacctccc gatataacag
    32161 gaaggattta cagaatgtga cctaaggtct ggcgtaaatg tgcaccggaa ccgagaagac
    32221 ccggatagtc atggacgatg agatacaccg gaatatcatg gacatattct ttaaagcgcc
    32281 ctttatcttc aaatgcggca cggaaaccgg aggctttgaa gaactcaagg aagcgcggca
    32341 cgataccgcc cgcaataaac acgccgccaa atgtcccgag attgagcgcc agattgccgc
    32401 caaaacggcc cataatgacg caaaacagcg acaatgcgcg gcggcaatcg gtgcagctgt
    32461 cagccagcgc gcgttcggta atatcttttg gcttgagatt ttctggcagg cggttgtcag
    32521 ctttcacaat tgcgcgatac aaattcacca gcccagggcc agaaagcacg cgctccgccg
    32581 aaacatgacc aatttccgca cgcaatattt cgaggataat ggcctcttct tcactattcg
    32641 gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac ccaacgctta tcgacatgga
    32701 ccagatgcgc aaccccaagc cccgttccgg caccgtaaac cgcaataggc ttaccttcga
    32761 ccggttctgc gccaccaaac tgaatcagat gctctttttt cagcatcggg atcaccatcg
    32821 atacagcggt aaaatcgtta ataatttcca gatggctaaa accgagattc tttttcattt
    32881 cggcaattga gaacgcccag gtatggttgg tcattgccac ccagtcaccg gtaattgggc
    32941 aagcgatggc aatacagccg tctttcacct cgaccttatg ttcttcaaga taaacgcgaa
    33001 tgaccgcttc gaggctgggg taatcaagcc ctgaataggt cttagcctgc gagatttcac
    33061 cactggcaat atcacacaga gcaagacgtg cgttggtgcc gcccacatca ccgactaatg
    33121 catactttgt cattcttcaa ctgctccgct aaagtcaaaa taattctttc tcacactgta
    33181 aatacctggg ggcataacaa caacgccgga aaggcaggct ccctgtaaat atcgatctgg
    33241 gtcacacaat tactttatcg tttcagcacc aattgcagcg atgccttttt gcaagctggg
    33301 caaactaagt atctgacccc gcataaggaa tagaacatgc tccatccgcg agccagaacc
    33361 atgttgttat tatcgctccc cgccgtggca attgggattg cgtccagtct tattctgatt
    33421 gtggtgatga aaatcgcctc ggtattacag aatttgctct ggcaacgact gccgggaact
    33481 ctggggatag cccaggattc acccctctgg atcatcggtg tattaacgct aacgggtatt
    33541 gcggtggggt tggttatccg tttcagccag ggtcatgccg gaccagaccc cgcctgtgaa
    33601 ccgctgatcg gcgcaccggt tccgccctct gcgctacctg gacttatcgt agcattaatt
    33661 ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc cgatcatgac cgtcaatatc
    33721 gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg tcaaccgaat ggagtggact
    33781 attttagcct ctgccggaac catcggcgcg ctgtttggta cacctgttgc ggcggcgttg
    33841 atattttcgc aaaccttaaa tggcagtagt gaagttccgc tatgggatcg tctttttgcg
    33901 ccgttaatgg cggcagcagc tggtgcactt actaccggat tatttttcca tccccatttt
    33961 tcactgccca ttgcgcatta cggacagatg gaaatgaccg atattctcag cggtgcaatt
    34021 gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat ggtgcttacc acggttgcac
    34081 gcgatgatga atcaaatgaa aaatccggtg ctcgtgctgg gtattggcgg atttattctc
    34141 ggaattctgg gggttattgg tggaccagtt tcgctgttta aagggctgga tgagatgcag
    34201 cagatggtgg caaatcaggc tttcagcacc agcgattact ttttgctggc ggtaattaaa
    34261 cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg gtgggcgaat cttcccggca
    34321 gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc acgttcccgc cgttccagcg
    34381 gcaataaccg tttcctgcgc tattctcggc atcgtgctgg tggtaacacg cgatggctgg
    34441 ttaagtcttt ttatggcggc agtcgttgta cccaatacca cattgctacc gctgctctgt
    34501 atcgtcatgc ttccggcatg gctgttatta gcaggtaagc cgatgatgat ggtcaatcgt
    34561 ccgaagcaac agccacccca cgataatgtt tagcaaaaaa tgctctcctt tatgatttaa
    34621 gagttatggc gatgatacgg ggctttacct ccccgtaata ttgcgttaac aggccgctga
    34681 cggcttcgat cgtgaaggag aataacaatg ttcaggtcac tgtttctggc ggccgccctg
    34741 atggcattta ccccgcttgc agcaaatgca ggtgaaatca ccctactgcc atcgatcaaa
    34801 ttacaaattg gcgatcgcga tcattacggt aattactggg acggtggtca ctggcgagat
    34861 cgtgattact ggcatcgcaa ttatgaatgg cgcaaaaacc gctggtggcg tcatgataat
    34921 ggctatcatc gtggctggga taagcgtaaa gcatatgagc gtggctatcg tgaaggctgg
    34981 cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg ggcaccgcca ttaaacgcgt
    35041 cctccaatgg agcacaatgc ctgatgcgac gctagcgcgt cttatcaggc ctacaaaccg
    35101 gctctttaac gcgtagggcg gataaggcgt ttacgccgca tccgccaata gtgccagatg
    35161 cgacgctcat tcaactacaa tcccagcgcc gtccccacca acaaccagat attcagcgcc
    35221 acgaccagca ctacaatcac ccagcctgtc tgttttacgc gtttgctgtt caccagatcg
    35281 cccatcaact tgctgtcact ggtgaaaatc agcagtggaa ccagcgccag ggcgatacca
    35341 aaacttaaca gcacctgact cataaccaga atccgtgtcg gatctaatcc catcagaatg
    35401 acaataaatg acggcaacat ggtgactgta cgacgcaccc acagcgggat atggaagcga
    35461 atgaatccct gcatcaccac ctgccccgcc agtgtcccca ccaccgttga ggacagtccg
    35521 gcagcaacca gacttaaccc aaagaccgtt gccgcagcat ggcttaacag cggttgcagc
    35581 gtcagataag cctcatcaag atcggcaaca ccagtatgac cagaaaagtg gaacgccgcc
    35641 gcagctgtag ccatcatcac cagattgaca aaaccggcaa tcgtcatggc gatagccaca
    35701 tcccatttgg tggcggaata acgttgttga cgcgaaccgc catgtaaatg ctgagtgagc
    35761 gaagagtgca aataaatcac atgcggcata atcgtcgccc ctaacacgcc tgctgccagg
    35821 aagaccgcct ccgaagtagg taaactcggg atcaccattc ctttacccag ctgcgccagg
    35881 ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa cagtaacccg
    35941 ccaatcactt tctccagcgg tttttgcccg cgacgttgca gcattaaaat caggaaagtc
    36001 gcgatccccg tcagcaccgc gccctgcaac aacgaaacac caagaatgag tttaaaaccg
    36061 atcgccgcac caataaattc cgccaggtcg gttgccatcg caataatttc tgcctgaacc
    36121 caatagaacc acactacggg acgcggatag tgatcacgaa tctgctccgc cagattttta
    36181 ccggtggcaa tccctagttt ggcagagagg atctgaatca gcatcgccat caggttggcc
    36241 caaacgacaa cccacagtag ctgatagccg aagctagcac ccgcctgaat attggtcgca
    36301 aagttaccgg gatcgatata accaatcgcc gcaatgaacg caggtcccat taatgcgagc
    36361 ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt catcttgtgc
    36421 ctctaaaaca tagcctttgc tatgtttcat gctatgctaa acgagaatga ttatcaaatt
    36481 catttaaatg gattgtggtg atttctctga tagaccaaga ttatgattac gaaaagattg
    36541 atgacttgaa tgttatggtt atgtttaatg ttagcacatt tacataactt tcagcttcca
    36601 tacacaacat agcagaaatg tatgacagat cactattttt gaagcctgtc acaggacgtc
    36661 attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg tgcacggaaa
    36721 tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg tactggcact
    36781 tgccgttgtt gtgattctcg cactgctggt aagcagcgac cgcaaaaaaa ttcgtatccg
    36841 ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc tgaactccga
    36901 cgttggttta ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc tcggatttgc
    36961 caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg cattcttctt
    37021 cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg atcggtattc tccagcacat
    37081 tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg ctctccaaag tcaacggtat
    37141 gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt cagaaaactt
    37201 tattgcctat aaagatatcc tcggcaaaat ctcccgtaat cgtatgtaca ccatggctgc
    37261 cacggcaatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca tgctggaacc
    37321 gaaatacgtc gttgctgcgc tggtactgaa catgttcagc acctttatcg tgctgtcgct
    37381 gatcaatcct taccgtgttg atgccagtga agaaaacatc cagatgtcca acctgcacga
    37441 aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca aagttgccat
    37501 tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga acgcactgtt
    37561 tgccaccgtt actggctggt ttggctacag catctccttc cagggcatcc tgggctacat
    37621 cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc aagtgggcag
    37681 tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc tgcagaaaat
    37741 tgcttccacg ctctctccgc gtgctgaagg catcatctct gtgttcctag tttccttcgc
    37801 taacttctct tcaatcggga ttatcgcagg tgcagttaaa ggcctgaatg aagagcaagg
    37861 taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg tgagtgtgct
    37921 gtctgcgtca atcgcagcac tggtgctgta aaaccataca taaaaaagcc ggggatcatt
    37981 ccccggcttt tttacgcctg ttaatcagct aatggctgcg ggcgaccaat caaataccct
    38041 tgcagatatt gcaccccgag cttatgcaat agcgcctgct gctgttgcgt ctcgacaaac
    38101 tccgcgacca cactcaatga cttcgctttc gccagatcgg taattgatct cacaatcatc
    38161 gcatccagcg tattcgtgac aatatctttc acaaagacgc catcaatttt gatgatatca
    38221 gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc atcaatcgca
    38281 atccggaaac caaacttatg cagctgctcg atgttgtaca tgctgctttc tgcgttagaa
    38341 aacgcctgct cctcggtgat ctcaagaatg accgcctgcg gggagatgtg ataacgctta
    38401 aacagacgaa taatccgccc ggcaatattc ttttgcagca gcgtgagcgg cattaaattg
    38461 actgaaaagc gcggtccttt tttgtcgcaa gggtgtgttg ccagccactt caacaaggat
    38521 tccagcactt gcaaatcaaa acgcgcgcta aggttaaact gagcaataag gggcagaaac
    38581 ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg cgaggatctc atcataacct
    38641 tcaccctctt tgttgcgaat tggctgggcg tagagcagca aatcaccctg atctaacgcc
    38701 gtgcgaatgg tattcagcaa tagcacctgt ttagtggtct gcccggaaac catctcctct
    38761 ctgctatcca gcgccagcac atgatgatgt gcgcaggatt gctccgccag ccagcttaac
    38821 tgccccaaca agggttgcag ggtttcctga tttccatcaa aacgccccca ggcagcacca
    38881 tagcccatat ccagcccggt attgttccag tgaatttgcc gactattcag gatgttaacc
    38941 atatgctgga gtcgcccttc cgtttccggc ccgctcagca ctaacagcag ttcactaccc
    39001 ggcaattgat acaacttttc gttttcctgc atcaacggca gcagcgtacg gcaaagccgt
    39061 aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaaactc ttgcccgcct
    39121 cttgctccgg cgcttgttcc aacgcacgaa agttgggtag gagcgttaac ggatctgtta
    39181 gcgcctgcaa atgccagcgg cgattaagcc attcactgcg atgataaatg cgcaccatat
    39241 agagcaggca aacgctaaag gaaatcagca ccgcgagaat aaatgccagc gaatattcgg
    39301 tttccacccc ttgcaaaaag ttctggttgt aattcagaag gcaaagcgtt gaaacagccc
    39361 aggtgagatt taaaaacgga tagcgaagat taccgacccc gagggtaaag atgatgaaga
    39421 aaacgggtac caggtaaccg gcaataaagt cgttttcata aggtgtgcac aacagaagta
    39481 gcagcacgct taacgctgcc agccagctta aggtaaatgc gagtgagata gtagaaaagc
    39541 atgttgtaaa tcagcactgc ggtgaaaagg cttagtaaat caacgaccgt gaaaatggca
    39601 tccgcatcgc cgaaaaaggt ggatatcttt agcgggaaat caaagaagct ccccacaaga
    39661 tacatgctgc atttgatgcc gatcggcgtc accaaaccaa gccagaacag acgttgccag
    39721 acatatcggc tggtcaatcc ataacgccaa cgcgtcccca gctgccagcg taatattgcg
    39781 caggcacaca acacgacaaa cgtctggctg ccgagcatga ccgcagtctg taaaaaagag
    39841 agatgaaagt tccacagatt ggtgcaaaac atcccgcata aaacgggaac gacaccgcgc
    39901 cagccaaaga taaacaagat tgacagcatt acgcacagtg gcatccacgc cagaaagata
    39961 taactggaat ggataatggc aagtggcgaa ataaaacggg atagctgaat aagtaccacg
    40021 gtgagcgtaa acgctagtgt gaatatcttg atatttttta tcaggttatg ctccacaaac
    40081 atgagtacac tactctcact gatcggggtt ttcaggcaca caaataataa gtttattcgc
    40141 atcgcgaagc aaacaggggc cagcctgttg ccgatttttc atccaatgat gctttcacca
    40201 ggcggcgcta acccggacgt agactgtatt acaaaagcgg caaaaagcag agacaaaaaa
    40261 cccccgcttt gcagcgaggg ttggaaattt ggtggagcta agcgggatcg aaccgctgac
    40321 ctcttgcatg ccatgcaagc gctctcccag ctgagctata gccccacgat gcgtttacgt
    40381 accaaatttg ctgggtgcaa aatttggtgg agctaagcgg gatcgaaccg ctgacctctt
    40441 gcatgccatg caagcgctct cccagctgag ctatagcccc gtcacgtaaa gcttgtcgag
    40501 ttgacgggcg gcatcatatg aattccgccc gaatgtgtca acggcaaatt gcaacgtgta
    40561 gtttcaatcg ctgaaaaatc aggcaaatga acaattttgg aaaaccgctc gcattcagta
    40621 gttattcatg tcacggtttc ctgtaaagtg gtgttataaa atgaactact aatagaccca
    40681 catacattca gggaattgtt atgttcaagg agaggatgac accagatgaa cttgccagac
    40741 tgaccggcta tagccgccag accattaata aatgggtacg caaggaaggc tggacaacgt
    40801 caccaaaacc tggcgtccag ggtggcaaag cccgactggt tcacgtcaat gaacaggttc
    40861 gtgaatatat tcgcaatgct gaacgtccag aaggtcaggg agaagcgcct gccctttctg
    40921 gtgatgcgcc gcttgaagta ttgctggtga cactggcgaa agagatgacg ccagttgaac
    40981 aaaaacagtt tacatccttg cttctgcggg aagggattat cggattgtta caacgcttag
    41041 ggattcgcga tagcaaataa tatgaaaaga ttacgcaata aaatgaccac cgaagaactg
    41101 gctgaatgcc tcggtgtggc taaacaaact gttaaccgtt ggaccagaga aaaaggctgg
    41161 aaaacggaaa aatttcccgg cgtgaaaggc ggtcgtgcca gacttattct ggtcgatacg
    41221 caagtttgcg agtttattca gaacacgcca gccttccata acacaccaat gttgatggaa
    41281 gctgaagaga gaattgctga atacgctcct ggagcacgcg ccccggcgta tcgacaaatc
    41341 atcaacgcga tagataatat gacagacatt gagcaggaaa aagtcgcgca atttttgtca
    41401 cgtgaaggaa ttcgcaattt cctcgcccgt ctcgatattg acgaatcagc ataaataaaa
    41461 aacggcagga tattatctcc tgccgtttat ctttttacac gctaattact gctgattttc
    41521 gcgttcagca ataaaatcca gcgctttgtt gatacgctcg atactgcggg tcttaccaat
    41581 tgcgtgaacg gtaacatcca gtgctggaga ctgccccgca ccggttacgg cgacacgcag
    41641 cggcatacca actttaccca tacccacttc cagctcatcc gccgtcgcct gaatagcgtg
    41701 atgaacgttt tcagcggtcc agtcagtaat cgcggccagt ttgtcacgaa ccacttccag
    41761 cggctgacgc gctaccggac gcagatgttt tttcgccgca tcggcatcga actcagcaaa
    41821 atcttcgtag aaataacggc agctctgtgc catctctttc agcgtcttgc agcgttcgcc
    41881 cagcagtttc accagatcag ccagctgcgg gccgttacgg gtatcgatat tttcctgctc
    41941 aatgtgccac tgtaagtgag tagcaacata ctccggcggc agcgcgttaa tgtagtgatg
    42001 gttcagccac agcagcttgt cggtgttgaa cgcactggca gatttgctga cggcattcaa
    42061 agtgaagtat ttgatcatct cttcacgagt gaagatttcc tgatcgccgt gggaccagcc
    42121 cagacgcacc agatagttca gcagtgcttc tggcaaataa ccgtcatcac gatactgcat
    42181 tacgctgact gccccgtgac gtttggacag ttttttaccg tcatcgccat tgatcataga
    42241 aacgtgcgcg taaaccggca ccggcgcttt cagggcctta agaatgttga tctggcgcgg
    42301 cgtgttgttg atatggtctt cgccacggat aacgtgggtg atttccatat cccagtcatc
    42361 gacaaccaca cagaagttat aggttgggga accatcggta cggcggataa taagatcgtc
    42421 cagttcctgg ttgctgaact cgatcggacc acggatctga tcgtcaaaaa caacagaacc
    42481 ttcctgcggg ttagcaaaac gtacaacaca cggttcatca tcagcatgat gctcatggct
    42541 gtggcggcag cgaccgtcat aacgcggctt ctcacctttc gccatttgct cttcgcgcag
    42601 cgcttccagg cgctctttag agcaatagca tttataagca gtgccctctt ccagcatctg
    42661 atcgatcacc gcgttgtagc gatcaaaacg tttggtctgg tagtacggac cttcatccca
    42721 ctccaggctc agccagttca tgccatccat aatggcttcg atagcttccg gcgtggaacg
    42781 ctcaagatcg gtgtcttcaa tacgcagcac gaacgcaccg ccgtggttac gtgcaaaaag
    42841 ccaggagtaa agagcagtac gcgcgccgcc aacgtgcaga tagcctgttg ggcttggcgc
    42901 gaagcgagtt ttgattttca tgaaatggcc ttacgtttag aaagatgccg acaaccggca
    42961 aatcctggaa aaattgagtg ggcgatattc tatcactccc gcctgattcc tcaatgtagt
    43021 tcgggtttct accttacgca ttattgtttt cgttgacaaa ttgcgcaaca cggtctgttt
    43081 tgcgatcatc ttgcttaaat ttacgacgaa cgaacaattt ctttagaaaa tgcgttgact
    43141 cattttgaac tctccctata atgcgactcc acacagcggg ggtgattagc tcagctggga
    43201 gagcacctcc cttacaagga gggggtcggc ggttcgatac cgtcatcacc caccaactac
    43261 tttatgtagt ctccgccgtg tagcaagaaa ttgagaagtg ggtgattagc tcagctggga
    43321 gagcacctcc cttacaagga gggggtcggc ggttcgatac cgtcatcacc caccacttcg
    43381 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
    43441 ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgatac
    43501 tcaaagattc gaacctgcag caggtttgag ttgagcgcag cgaaacaacg gagccgctcg
    43561 cggcgacggc ccgaagggcg agcgaagcga gtcatcctgc acgacccacc aatgcaaaaa
    43621 agcgccctaa aggcgctttt ttgctatctg cgatttgtaa gattgatttt tcttctcaag
    43681 ttccttgttc ttctaccgat attacctcgt acagtgacga ggactgaaca atggccacta
    43741 tctcttcaac ttcaattcct tccatacaaa cacagagtag taacagggca tcacaaggta
    43801 gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat catcaaactc actcaacaaa
    43861 ttaaagagat tgtagacact agcggcagtg ctgaagacaa gcaaaaacag gccgaattga
    43921 ttcaacagca aatcacccta ctggaaacac aactggcgca gttgcagaaa cagcaggcag
    43981 aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt atcccttcta aatcctgttg
    44041 aaaaaacgac tcatatcgat atctatatct gatacaacgg atccccttcc cgaccagcca
    44101 ttagcgcatt gccctggtct cgtaccacgt cccataacgc ttcagcggcg gtggaaagtg
    44161 aacggttctt cctgcgcacc agcatcaact ggcgttcaac caccggcgta atgcgcttaa
    44221 ccaccagtgg gctaccttca ggcaatggta gcgccagtgc aggaagaata ctgatgccga
    44281 tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc aatctcctgc acaatattcg
    44341 cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct gacgcgtaat
    44401 cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct tccacggcca
    44461 aagcgctatc gcgatggcaa agcagaaaga aaggctcgga aagaatcgct tcacattgca
    44521 gatcgcccac tgggcctgga tcgatgacga tgccaaagtc gacatctccc tggcgaatac
    44581 tttccatcac ccactgctgc ggacgatcat gcaggacaaa ctgaatatct ggatagcggc
    44641 gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcga
    44701 cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa
    44761 gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca
    44821 cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac
    44881 tgtgacttac tgccgactgg ctcaagccga tacgctctcc tgcacgacta aaacttttct
    44941 cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc tgttttcttc
    45001 atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat
    45061 cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg
    45121 atcacggtgg tgttgctggc ctctttcttt ccggccagag gcgatttcgt ccccttcttt
    45181 gaaaatctga ccaccgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg
    45241 cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt aatgtgcagc
    45301 accttcgtgc tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc ggtaaatgtc
    45361 gacccgatgc tctactccgg ttttctctac ttgtgcattc tcccggctac cgtgcagtct
    45421 gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg ttctgcgtca
    45481 gcatccagcc tgctggggat tttcctttca ccattgctgg ttggtctggt gatgaatgtt
    45541 cacggtgcaa ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact gctgctgccg
    45601 tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa
    45661 tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacagc gttcagcgaa
    45721 gccgtcgtta atggtatctg gcataaagtt ggctggggat cattgctgtt tatcgtggtg
    45781 gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg
    45841 agcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca
    45901 aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg
    45961 cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac
    46021 aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa agcttaaagc
    46081 ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt
    46141 tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga
    46201 tactcctccg gcaggtcaat taagcgacgc cctttgtatt tgccaaacgg cattatcgta
    46261 ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc
    46321 cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag atcctgcagc
    46381 ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag
    46441 ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac
    46501 cacggtttta ccaacaaacg ggctgtcaat ctcttccgcg ttgataacga tcggcgcagg
    46561 ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa
    46621 gaagttgtga acgtgggatg caacgacaat gccaacatca ggcacctttt gcagctcttc
    46681 aatcgaagcg gcttccagcg cttccagcgt gccgaaatat gccgccagac ctgctgcggt
    46741 ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt
    46801 cgctttttcc agcgcgttaa ccacgttttg tgccaatttt ggccccatac gctccagtcc
    46861 ggtcagtttg cctgcggtga gtttgaacag atctgccgga gtatggacat attctttttc
    46921 aaccagctga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac
    46981 aaagtgtttc agcgactctt tacgctgcgc accgcaaatc aggccaccgg tacagcgggc
    47041 aaccgcttca ccttccacac gctcaacgtc agaaccacat accggacaat gcgtcgggaa
    47101 tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca cctgcgggat
    47161 cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa gacgttcgat
    47221 ttcatccgca ttgtgtaagg ttgcgttact caccagcacg ccagcaacat ggacaggttc
    47281 cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac
    47341 aaaggtcatc tgctcctgcg ccgggaattt aaacgcaacc gcccagcgcg gggcacgcgc
    47401 gacaaagcca agctgctcct gctgttccag tgagttgacc ttaatcacca cgccgtcgat
    47461 atcaaagccc agcgtcgggc ggtcttcttc cactttgtga tagaacgcca gcacttcttc
    47521 cgccgattca caaagcgtta cccgatcgct gaccggcacc ccccactttt taaattgcag
    47581 taaacggcca agatgagtat ccggcagctc gccaccttcc agaacaccaa cgccatagca
    47641 gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc
    47701 tgccgcatta cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat cttcgttaat
    47761 cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat
    47821 attctctccg tgcagcttca gcggaatggc gcgaatagta cgcacattag acgtgatatc
    47881 ttccccggtg gtgccatcgc cacgggtcgc ggcactgact aaaacgccat tttcatacag
    47941 aatactgacg gcaaggccat ccagcttcag ctcacagcac caggtgactt tctcgttgtt
    48001 tttcagacgg tcctgcacac gtttgttgaa agcaagaaag ctttcttcat caaaaacgtt
    48061 atccagtgac agcattggta cttcatggcg tatctggctg aaagctgcca gcggcgcagc
    48121 acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc
    48181 gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac
    48241 atgataaaga tattcatgat ggcaaagcgt cgttcgcagt tctgtcagtt gttgttcgat
    48301 tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag
    48361 ttaatttgcc ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg atgtcctggt
    48421 actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg acaccgccca
    48481 cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac
    48541 cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag
    48601 gatcaaaggt tcccggtttc accatattcg ccaggctgaa taacgccggg ccgctgccat
    48661 ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa
    48721 tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga
    48781 taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct
    48841 caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg
    48901 ctgaatgcac aggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct
    48961 gttcaggctg cggctgatag gcaggctgct gcaccggctg cgctggacgc ggagcatgtt
    49021 gcggcggtac ctgcgcttca ggcggctgct ggaccggttg acgcggctgc gcagacgcat
    49081 aaggcggtag gtactggtgt tgcggcgacg gacgagcagc ctcatgctcc tgagcgttag
    49141 ccggggcatg attcacgcgg tgaacacgaa cctcaccaac gccctcatca tcttcgacat
    49201 cctcgtcata agaatcgtcg tcacgttttg acttcattcg ttttaatggc cgatcgcgga
    49261 acatagaaga tcgttcttta cggctggtcc agaaaccatg taccagtaaa gcgattatgg
    49321 cgatcgcgcc aacaatgatt aatatcagac gcaaatcctg catcattata ttctctgttg
    49381 ttctaacacc ttgccaccac ggcaaacatt tactcactaa gagtatttgc cgattacctc
    49441 aagtgcaagt gcactattaa ctttcacagc acaaagatag atgaaatcgt gctttttgct
    49501 gttttttcga acatatccta actgtccatt gcgcaattac ccggtaaaat acgcagaatt
    49561 ttcctgggat tggtcaaaag gagctcatcc tgattatggt ttcatcattc acatctgccc
    49621 cacgcagcgg tttttactat tttgcgcaag gctggaagct cgtctcgcaa cctgggattc
    49681 ggcgtttcgt tattttaccg ctgctggtca atattttgtt gatggggggc gcattctggt
    49741 ggctctttac acagctcgat gtctggatcc cggctttcat gagttacgtt ccggactggc
    49801 tacaatggct gagttatctg ttgtggcctc tggctgtcat ctctgtgctg ttagtgtttg
    49861 gctatttctt ctccacgatt gctaactgga ttgccgctcc gtttaacggt ttattggctg
    49921 aacaactgga agcacgattg actggcgcta caccgccaga taccgggatt ttcggtatca
    49981 tgaaagatgt gccgcgaatc atgaaacgcg aatggcaaaa atttgtctgg tatctgccgc
    50041 gcgcaattgt attactaatt ctttacttca tccctggtat tgggcaaacc gtcgcgccgg
    50101 tactgtggtt cctgtttagc gcctggatgt tagccatcca gtattgcgat taccccttcg
    50161 ataaccacaa agtgccgttt aaagagatgc gcaccgccct gcgcacacgc aaaatcacca
    50221 atatgcagtt tggtgcttta accagcctgt ttacgatgat cccgctgctt aatctgttca
    50281 ttatgcccgt tgccgtttgt ggcgcgacgg cgatgtgggt cgattgctat cgcgataaac
    50341 acgcgatgtg gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt gaaacagggg
    50401 tggcttatgc cgccccttat tccatcttgc atgtcattat ttcccttctg tatatagata
    50461 tgctaaatcc ttacttccgc atattctctg agcgggtatg ctacctgttg tatcccaatt
    50521 tcatacagtt aaggacaggc catgagtaag atttttgaag ataactcgct gactatcggt
    50581 cacacgccgc tggttcgcct gaatcgcatc ggtaacggac gcattctggc gaaggtggaa
    50641 tctcgtaacc ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat ttgggatgcc
    50701 gaaaagcgcg gcgtgctgaa accaggcgtt gaactggttg aaccgaccag cggtaatacc
    50761 gggattgcac tggcctatgt agctgccgct cgcggttaca aactcaccct gaccatgcca
    50821 gaaaccatga gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa cctggtgctg
    50881 acggaaggtg ctaaaggcat gaaaggcgca atccaaaaag cagaagaaat tgtcgccagc
    50941 aatccagaga aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc tgaaattcac
    51001 gaaaaaacca ccggtccgga aatctgggaa gataccgacg gtcaggttga tgtatttatt
    51061 gctggcgttg ggactggcgg tacgctgact ggcgtcagcc gctacattaa aggcaccaaa
    51121 ggcaagaccg atcttatctc tgtcgccgtt gagccaaccg attctccagt tatcgcccag
    51181 gtgctggcag gtgaagagat taaacctggc ccgcataaaa ttcagggtat tggcgctggt
    51241 tttatcccgg ctaacctcga tctcaagctg gtcgataaag tcattggcat caccaatgaa
    51301 gaagccattt ctaccgcccg tcgtctaatg gaagaagaag gtattcttgc aggtatctct
    51361 tctggagcag ctgttgccgc agcgttgaaa ctacaagaag atgaaagctt taccaacaag
    51421 aatattgtgg ttattctacc atcatcgggt gagcgttatt taagcaccgc attgtttgcc
    51481 gatctcttca ctgagaaaga attgcaacag taatgccagc ttgttaaaaa tgcgtaaaaa
    51541 agcacctttt taggtgcttt tttgtggcct gcttcaaact ttcgcccctc ctggcattga
    51601 ttcagcctgt cggaactggt atttaaccag actaattatt ttgatgcgcg aaattaatcg
    51661 ttacaggaaa agccaaagct gaatcgattt tatgatttgg ttcaattctt cctttagcgg
    51721 cataatgctt aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa tggactgtat
    51781 tgcgctcttc gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg ctaaagtcga
    51841 accgccaggc tagactttag ttccacaaca ctaaacctat aagttgggga aatacaatgt
    51901 tccagcaaga agttaccatt accgctccga acggtctgca cacccgccct gctgcccagt
    51961 ttgtaaaaga agctaagggc ttcacttctg aaattactgt gacttccaac ggcaaaagcg
    52021 ccagcgcgaa aagcctgttt aaactgcaga ctctgggcct gactcaaggt accgttgtga
    52081 ctatctccgc agaaggcgaa gacgagcaga aagcggttga acatctggtt aaactgatgg
    52141 cggaactcga gtaatttccc gggttctttt aaaaatcagt cacaagtaag gtagggttat
    52201 gatttcaggc attttagcat ccccgggtat cgctttcggt aaagctctgc ttctgaaaga
    52261 agacgaaatt gtcattgacc ggaaaaaaat ttctgccgac caggttgatc aggaagttga
    52321 acgttttctg agcggtcgtg ccaaggcatc agcccagctg gaaacgatca aaacgaaagc
    52381 tggtgaaacg ttcggtgaag aaaaagaagc catctttgaa gggcatatta tgctgctcga
    52441 agatgaggag ctggagcagg aaatcatagc cctgattaaa gataaacaca tgacagctga
    52501 cgcagctgct catgaagtta tcgaaggtca ggcttctgcc ctggaagagc tggatgatga
    52561 atacctgaaa gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc tgcgcaacat
    52621 cctgggcctg aagattatcg acctgagcgc cattcaggat gaagtcattc tggttgccgc
    52681 tgacctgacg ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg gtttcatcac
    52741 cgacgcgggt ggccgtactt cccacacctc tatcatggcg cgttctctgg aactgcctgc
    52801 tatcgtgggt accggtagcg tcacctctca ggtgaaaaat gacgactatc tgattctgga
    52861 tgccgtaaat aatcaggttt acgtcaatcc aaccaacgaa gttattgata aaatgcgcgc
    52921 tgttcaggag caagtggctt ctgaaaaagc agagcttgct aaactgaaag atctgccagc
    52981 tattacgctg gacggtcacc aggtagaagt atgcgctaac attggtacgg ttcgtgacgt
    53041 tgaaggtgca gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg agttcctgtt
    53101 catggaccgc gacgcgctgc ccactgaaga agaacagttt gctgcttaca aagcagtggc
    53161 tgaagcgtgt ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg gcgacaaaga
    53221 gctgccatac atgaacttcc cgaaagaaga gaacccgttc ctcggctggc gcgctatccg
    53281 tatcgcgatg gatcgtaaag agatcctgcg cgatcagctc cgcgctatcc tgcgtgcctc
    53341 ggctttcggt aaattgcgca ttatgttccc gatgatcatc tctgttgaag aagtgcgtgc
    53401 actgcgcaaa gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta aagcgtttga
    53461 cgagtcaatt gaaatcggcg taatggtgga aacaccggct gccgcaacaa ttgcacgtca
    53521 tttagccaaa gaagttgatt tctttagtat cggcaccaat gatttaacgc agtacactct
    53581 ggcagttgac cgtggtaatg atatgatttc acacctttac cagccaatgt caccgtccgt
    53641 gctgaacttg atcaagcaag ttattgatgc ttctcatgct gaaggcaaat ggactggcat
    53701 gtgtggtgag cttgctggcg atgaacgtgc tacacttctg ttgctgggga tgggtctgga
    53761 cgaattctct atgagcgcca tttctatccc gcgcattaag aagattatcc gtaacacgaa
    53821 cttcgaagat gcgaaggtgt tagcagagca ggctcttgct caaccgacaa cggacgagtt
    53881 aatgacgctg gttaacaagt tcattgaaga aaaaacaatc tgctaatcca cgagatgcgg
    53941 cccaatttac tgcttaggag aagatcatgg gtttgttcga taaactgaaa tctctggttt
    54001 ccgacgacaa aaaggatacc ggaactattg agatcattgc tccactctct ggcgagatcg
    54061 tcaatatcga agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt gatggtattg
    54121 ctatcaaacc aacgggtaac aaaatggtcg cgcctgtaga cggtaccatt ggtaaaatct
    54181 ttgaaaccaa tcacgcgttc tctatcgaat ctgatagcgg cgttgaactg ttcgtccact
    54241 tcggtatcga caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct gaagaaggtc
    54301 agcgcgtgaa agttggcgat actgtcattg aatttgatct gccgctgctg gaagagaaag
    54361 ccaagtctac cctgactccg gttgttatct ccaacatgga cgaaatcaaa gaactgatca
    54421 aactgtccgg tagcgtaacc gtgggtgaaa ccccggttat ccgcatcaag aagtaattct
    54481 tgccgcagtg aaaaatggcg cccatcggcg ccatttttta tgcttccgcc agcggcggca
    54541 aaatcaattc atcgctctca tgctgctggg tgtagcgcat cacttccagt acgcgcaacc
    54601 ccgcacggtg caccgcatcg tttaacgcct tccctttcag caaaccactg ataagctgag
    54661 cacaaaacag gtcgccagtc cctttcaggt cggtttttac ccgtgaatgg gaaatgacat
    54721 tcacgctgtc ggcactgacc accaccacct gcatctcctg attttcttca ttaccggagg
    54781 cgctggtaat caccacccat tttaatgtgt ctgaaagcag actttttgcg gcagcaatgg
    54841 cactgtcgag atcgcggcaa tttttaccgg tcaggatttc cagctcaaag atattggggg
    54901 taattccctg cgccagcggc agtaaatatt gtcgatacgc ctcgggaagg tcaggtttga
    54961 cataaattcc gctatcaata tcgccaatca ccggatcgac catgatcaat aggtcaggat
    55021 ggtctttgcg tagcgcagtc agccactcgg caaggatttt gatttgcgat gccgttccca
    55081 tatagcccgt ggttacagca cgaagttggc gcagcgcatc acgctcctga agcgcacgca
    55141 aatagccgct aaaccattcg tccggaatcg caccaccgta gaaagtgtca taatgcggcg
    55201 tattgctcag caataccgtc ggcacggcaa agacattcaa gccgttctgt tttatagcag
    55261 gcaccgcaat gctgttgcct acgcttccgt acactacctg cgactgcacg gcgacgatat
    55321 ccgcctgcag cgccctactc ttatcgttaa acaacaacaa actactcatt taattttttc
    55381 tccttgccga tgatcctcat cgtattccaa ccgaaacttt acctgattct ggcagtcaaa
    55441 tcggctatta caaaacaaag ataaggtaat tcagtgaaga aaatcatttg tctggccatt
    55501 acgctgctaa tgacactccc cgcttacgcg aagttaactg ttcatgaaga agcccgcatc
    55561 aacgccatgc tggagggatt agcacagaaa aaggatttga tatttgtgcg caacggtgat
    55621 gaacatacct gcgatgaagc ggtttcccac ctgcgtctga agctcggcaa tacccgtaac
    55681 cgcattgaca ctgccgagca gtttattgat aaggtagctt cgtcgtcatc gattaccggg
    55741 aagccgtata tcgtgaagat ccccggtaag agcgatgaga acgcacagcc ttttttacat
    55801 gcgttaattg cgcagacgga taaaacggtg cctgcggaag gaaattaatc cgctttggta
    55861 aggcatttac aggaggtaac atgaaaaaat gctttattta tcacgatgaa aaatcgaata
    55921 aattttggtg gatagattac gaaggggata gtttagctgt caactatggc aaggtaggta
    55981 gtattggtaa attccataca aaagaattcg ataatgaaga acagtgtctg aaagaagcca
    56041 gtaaattgat tgccgcaaaa atgaaaaaag gatatcaaga aactccagac tttaacttca
    56101 tggatctcta ctattttgat gatgaagaga ttgggttaca tcttaaaacg tcacacccaa
    56161 acttccagtg ccattttact gatccacttt atatgtgttg ctgggatgaa gaatctcctt
    56221 ttggtagcga tgaaggtgct gatgctctaa acgaacttga aaatagcctc cgttctggac
    56281 tgtgctgatt tccctcaaat gttaattgaa actatgtggg gtatgaaata catcgctatg
    56341 gacagtattc ttgaagagga tgtgcgtgcg caattactag ccaatgaaat gagctctatc
    56401 cagagcaata tgattaccta cgcgactgca ttcggtcaga tcaaagtcat gggtaaaatc
    56461 tcccataaac ttaaaaagat gggactcaat gcactagcgc gtcatcagct taccgcaaaa
    56521 attcttcaat ggggtgacgg tcaggactca ccaatacttc aaaaaatgat tgatgacctt
    56581 actgcttttc cacacgaaaa ttaaaaactg catttgtcgg cagcaacaac tgccgacaaa
    56641 tcagattatt gacaaagtgc actttgttta tgccggatgc ggcgtaaacg cctgatctgg
    56701 cataaaaata ttgctaattc aatatattgc agaaacattg taggcctgat aagcggagcg
    56761 catcaggcag ttttgcgttt gtcatcagtc tccaatgcta ttaatcctta aatccctgcc
    56821 cccagactaa aatgctcttc cccaaacact ccggtggaga ggtagcgatc gccacgatcg
    56881 cagatgatcg ctaccaccac cgcaccaggg ttagcttttg ccacccgcag tgctccagca
    56941 accgcgccgc ctgagctgac gccacagaat attccttccc gcaccgccag ttcgcgcatg
    57001 gtgttttccg catcgcgctg atgaatatcc agcacctcat ccaccagcgc agcgttgaaa
    57061 atccccggca gatattccgc aggccagcgg cgaatgcccg gaatactgct gccttcttcc
    57121 ggttgcaggc caacaatggt caccggtttg gattgttcgc gcataaagca tgaaacgccg
    57181 gtgatagtgc cggtcgtccc catgctggag acaaaatgag tgatgcgccc gccggtttgc
    57241 tgccagattt ccggcccggt ggtggtgtaa tgcgcataag ggttatcggg attattgaac
    57301 tgatcgagca gctttccttc gccacgattc gccatctcca gcgccagatc gcgcgcacct
    57361 tccatgccct gctctttggt gacaagaatc agttccgcac cataagcacg catcgccgca
    57421 cggcgttcct ggctcatgtt gtcgggcatc agcaatttca tgcgatagcc tttcagcgcg
    57481 gcaatcattg ccagcgcaat gccggtgtta ccactggtgg cttcgattaa gacatcaccc
    57541 ggtttaattt ccccgcgctt ttccgcctcg acgatcatcg aaagtgccgc acgatctttc
    57601 accgaacctg ccgggttatt gccttccagt tttaaccaca cttcactgcc gttatccggc
    57661 cccattcgct gcaacttcac cagaggcgta ttgcctattg tttgttctaa tgtactcaca
    57721 atctctatcc atacgtggtg ttgcctgatg cgacgcttgc gcgtcttatc aggcctacag
    57781 gttacaaacc ttgccattaa aaagcccgga tcgcgggaag cgcctccggg cgtttaacat
    57841 tcactcaacc tatcaggcgc tttgtgcgag agcaagttcc tcatcgcggg tttcaatacg
    57901 ctcgtcgccg ttatacagcc gcgcatgttg cagaccaacg aataaacgct cgccatgctg
    57961 cggggcatcg tcgccatgca tcacgaccgt cagcggttcg ttgtaccacc ccagcggctg
    58021 caccactaat tgggtgtagt gacctttcgg gctggcttcc agtacctgta ccggcagcgg
    58081 cgaatcgaga ctggtacggc ggctgatatc cacttcccac gggcgcagga agagatccac
    58141 cggcccctga tacgcaggtg tgtagcccag cggccagcga tgcgcgccaa catggaactg
    58201 cccgccgcga atggttccct gcaggcggtt cacttcgccc ataaattcga gtacaaaacg
    58261 ggtcgctggt tcgcgccata cctgattcgg cgcatcagcc tgttcaatat tcccctgact
    58321 catcaccact acacgatcgg ctacttcggt cgcctcttcc tggtcgtggg tcacaaacac
    58381 gctggtgaat ttcagctctt catgcagttg acgcagccag cgacgcagct ctttacgcac
    58441 ctgcgcatcc agcgcgccga acggttcatc aagcagcaga atttgcggtt ctacagccag
    58501 cgcgcgcgcc agcgccacac gctgtttctg gccgccggaa agctgcgccg gataacgatc
    58561 cgccagatgc gcaagctgga ccatttccag caattttgtc actttcgctt tgatggctgc
    58621 ggcattcggg cgctcgcgac gcggcagcac cgtcaggcca aaagtgatat tgtcgaatac
    58681 cgtcatatgg cggaacagcg cgtaatgctg gaacacgaaa ccgactttac gatcgcgcgc
    58741 gtgcaggcga ctcacgtcgg tgccgtggaa gcgaatatgc ccgctggttt gatgctccag
    58801 cccggcgata atccgcagca gcgtggtttt cccggaaccg gacggcccca gcaacgcgac
    58861 catctgacca gaaggaatat ccagtgagat atcgttcagc acctgggtgc gaccaaaaga
    58921 cttcttgata ttggcaatct caatgctcat gatgctcctc ctgctgcgcg cgtttttcct
    58981 gattctccag gcgccactgc aacatactct ttaaaaacag ggtgataatc gccatcaacg
    59041 tcaacagcgc ggcggcggta aaggagccga cggtgttgta gtcctgctcc aacaattcaa
    59101 tctgtaacgg cagcgaaagt gtttcgccgc gaatcgagcc ggaaaccacc gacactgcac
    59161 caaactcgcc aatcgcgcgg gcgttggtca gcaccacgcc gtaaagcagc gcccagcgaa
    59221 tattcggcaa tgtgacgcga cggaacatct gccagccgga cgcgccaagc aaaatcgccg
    59281 cttcgtcttc ctggctgccc tggcttaaca tcaccggcac cagctcgcgc accacaaacg
    59341 gacacgtcac aaagatggtg accagcacca ttccgggcca ggagaacata atttgcagat
    59401 tatgttcatc cagccagccg ccgagcgggc cgttggagcc gtagaacagc agatacacca
    59461 gaccggcaac caccggcgat acagcaaacg gaatgtccag tagcgtcagc agtaactggc
    59521 gtcccgggaa gttaaagcgc gtcaccagcc aggccagcag aatgccgaac accaggttta
    59581 ccggtacggc aatcagcgcg atcatcaccg tcagccagat ggcgtgcagc atgtccggat
    59641 cggccagatt ctgtaaaacc ggcatcagcc ccttgctgaa tgcctgcacg aagatgtaaa
    59701 tcatcggcac cagcaggatg aacgccgaaa ccagcatccc gatgccaatc agaaaccatt
    59761 tgccccagtt aatcgggcgc gcgtcataac gcttcaattg ggtaacttcc gccattaatg
    59821 acctaccaca cgccgaccaa agcgactttg cagagtgtta attgagaaca gcagcagcag
    59881 agatgccgcg aggatcaccg aagcaatcgc gctcgctgcc gggtaatcaa actcctgtaa
    59941 gcgcacaaaa atcatcagcg acgtcacttc cgtcttccac gcgatatttc cggcgataaa
    60001 aatcaccgcg ccaaattcac caagactacg ggtaaacgac agcgtcacgc ccgccaccag
    60061 cgccggagaa agctccggca gcaccacttt gcagaaactc tgccagcgcg ttgcaccaag
    60121 cgtttccgcc gcttcttcat attccgggcc taactcttcc agcaccggct gcacggtacg
    60181 caccacaaac ggaatgctgg taaaggccat cgccaccgca ataccaagcc aggtataggt
    60241 gactttgata tcaaacttcg ccagccattc accgtaaaaa ccgtttacgg aaaagagcga
    60301 ggccagcgtt aaaccggcga ctgccgttgg cagcgcaaag ggtaaatcca tcagcgcatc
    60361 aagcagcgtg cggcccggaa agcgataacg tgttaaaatc cacgccatca gcagaccgaa
    60421 aacgccgtta aaaatcgatg ccacaaacgc cgacagcagc gtcactttgt aggccgcgac
    60481 cacctgcggg ttggtgatca cctcccagta ctgcgcccag ctcatctcag aaagctgcat
    60541 caccagcgca gagagcggca gcagcaatat caggcacaca aacagcagac tggtgccaag
    60601 gcttaaagta aagcccggca gcacgcgtct tgaagaaact gcaaacatta cttacgcccc
    60661 gccgctaaca gcttgtctaa ctcgccgccg ctggtgaagt gggttttcat cacttccggc
    60721 catgagccaa atttgtcttc cacgcggaac agttcggtct gcgggaattt atctttcagt
    60781 ttgtccatga cttccgggtt attcacacgg tagtaatagt cggtgatgat ggtctgcgcc
    60841 tgtgggctgt agagccagtt caggtaggct tttgcggctt tttccgtacc gttggcctgc
    60901 acatttttat cgacccacgc caccgggaat tccgccagaa tattggtttt cgggatcacc
    60961 acttcaaagc cctgtgcttc atactgttta cggatgttgt tcacttccga ttcaaagcta
    61021 atcagcacat cgcccaagcc acgctcggca aaagtggtgg ttgcgccacg accgccggta
    61081 tcgaacactt caacgttttt caggaactgg gtcataaact gttcggtttt ggctttatcg
    61141 ccaccgtcag ctttatccgc tgcgccccat gccgccagat aggtataacg cgcgttaccc
    61201 gacgttttcg ggttcgggaa aatcagcttc acgtcggagc gcaccaggtc gttccagtcg
    61261 tggatattct tcgggttgcc cttacgcacc aggaagccca tggtggagta gaacggcgag
    61321 ctattattcg gcaggcgcga ctgccagtca gccgggatca gcttgccttt gtcgtgcagg
    61381 atttgtacgt cggtcacctg gttataagtg acaacgtctg cttttaagcc ctgcaaaatc
    61441 gccagcgcct gttttgatga cccggcatga gattgtttta tcgtcagttt gtcgccgccg
    61501 ttatcttttg cccattgctg ttcaaacggc ggattcaggg cggcaaacag ctcgcgggag
    61561 acgtcataag aactgttcag cagttccgtt gcctgtacat ggcccgccag cagcagagaa
    61621 gcgaccagcg cgagtgagtt ctttttcagt aagttaacgg ccattgcgca cccttataaa
    61681 tttaatgact ttctaatagc catcatattt ataacggacg tgaaaggagt aacggtttta
    61741 tataccgttt ggtgatttgg aagttaaaaa gggaataaga acttggttca acaggcgtaa
    61801 ttgttgcagg cagtttggac acggacagcg cggagaaacc ggagcgtaca catagtacgt
    61861 gaggatttcg agcactgccc ggggccaaaa tgacaaatga aatagcctgg tgaacttagt
    61921 tcaagacgaa atcctcccca cacatgcagg gaggaaacag aggtgaatca gataccgacg
    61981 ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag
    62041 ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg atcggcgagg
    62101 cgacacagcg ggattgcttt tgccatttca gtcagcaccg attctggatc ttccgggttc
    62161 gactggcggg caatgctttc cgccattggc gtgcggacgt atcccgggca gatggcgtta
    62221 acgcgaatac cggattgcgc gtactccacc gccagcgatt tagtcaggcc aacaatcgcc
    62281 gctttcgtta aggcatacgc cgtttcgcca ggatcggcca ccatatcacc agtgactgaa
    62341 gacatcatca caatgcgacc atctttgcgc gcaatcatct ccggcagcac cgccttcgtg
    62401 acgttccata cgcctttaat attgatatca atatggaaat cgcgatcttc atcgctcata
    62461 tcgaggaaac tgcccagacg acaaacgcct gcgttattca ccaggatatc aatgcgccct
    62521 tctttttcct tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg cacatcggcg
    62581 acaaccgccg tacagcgatg accacgacca cacagttcgt ccgctagctt ttcgatctca
    62641 ggggagatat ccagcaagat taggttcgcg ccatgacgtg caaaagttct ggcaattcct
    62701 tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt
    62761 ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata
    62821 aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaatcc agtcgctgct
    62881 ttatgcatca tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt ttcaatgacg
    62941 ccacatcatt gatgttgcac aagaccaaca aacagtaaat cggtaacaga attttgcgcc
    63001 gtgcgggtag acatcgacga gctacgccat tcggtttcac cggaaacggt atcgagcgtg
    63061 aaatgcgcca gccgccgcag cggagagtca gccagagaag taatggcaat caccgttgcc
    63121 ccctgctttc gtgccgcttc tgcgcacaag acaatctctt ttttactgcc gctgtaagag
    63181 atagcgattt gtacatcgcc ttttttcaac gcctgggaaa cggtggcttg tacgtgcgtg
    63241 tcggcttcgc aggccacgcg atagccaatt ttcatcagtt tgaacgacag atcgcgcccc
    63301 accagcgccg atccgcccag gcccgttatc tggataaacg gtgctttact gatgacctca
    63361 atgatttttt gcagccgcgc gtagtcgaac aacgcacagg tttgctccag cgccagctct
    63421 ttttcgcgat tcagtttgcg ggcaatcact tccagcgaat catcactggt aatcgaactg
    63481 tgcaggtgca gcgccgtggc attcgttttc tcccggctgg cactgtattc gccaattaac
    63541 gccatacgca attcagtaaa tccttgcgcg ccgagcttct gggcaaattt cacaatgctc
    63601 gactggctga taccgagctg tttcgccatc tgccgtgaag agacggattt cagctcgctc
    63661 actctggccc gcagaaagtc agcaattttt tgctcgttcc cggtaaattc actttccgca
    63721 ttgcgtattt tcgtcaggta caacatcttc cactctcctg ggctggcagc aaaaattccg
    63781 cttaagttct tatgacgctc ttcacactct gcgagtgtag actcaatgat tcctttaagt
    63841 ctgtcaattc gggatatatg attccataca tctgcaatta tttgatcgta aatagtaagg
    63901 tcaccaccga tgcaactaga aaagatgatt actgaaggct cgaacaccgc ctcggctgaa
    63961 attgaccgcg tatcgacgct ggaaatgtgc cggattatca acgatgaaga taaaaccgta
    64021 ccgcctgccg ttgatcgcgt cctgccggat atcgccgcgg cgatcgatgt tatccacgcc
    64081 caggtcagcg gcggcggtcg tctgatttat ctcggtgcgg gaacatccgg tcgtctgggg
    64141 attctggatg ccagcgaatg tccgcccacc tacggcgtga aaccgggtct ggtggttggt
    64201 ttgattgctg gaggcgaata tgccattcag cacgcggtgg aaggagctga agatagccgg
    64261 gaaggtggtg ttaacgatct gaaaaatatt aatttaacgg cacaggatgt ggtggttggc
    64321 attgctgcca gcggtcgcac gccgtatgtg attgccggac tggaatacgc acgccagctc
    64381 ggctgccgca cagtgggaat ttcctgtaat ccggggagcg ccgtttcaac caccgctgag
    64441 tttgccatta caccgattgt aggtgccgaa gttgttaccg gttcttcgcg gatgaaagca
    64501 ggtacagcgc agaaactggt gctcaatatg ctttccaccg ggctgatgat taaatccggc
    64561 aaagtgttcg gcaacctgat ggtcgatgtg gtcgccacca acgaaaaact gcatgtgcgc
    64621 caggtcaata tcgttaaaaa cgccaccgga tgtaatgccg aacaagcgga agcggcgtta
    64681 attgcctgcg agcgcaactg taaaacggcc attgtgatgg tgctgaaaaa tctcgatgcc
    64741 gcagaagcta aaaaacgcct ggatcaacac ggcggcttta ttcgtcaggt tttagacaag
    64801 gaataaccca tggccaaaga gatcagcagt gaacttctga acaccattct tacccgtgtc
    64861 ggcggaccag gaaatatcgc cagttgtggt aactgtatga cgcgcctgtg tctgggtgta
    64921 catgacagtt cactggttga tcccaatatc aaaacgctgg aaggcgtgaa gggcgtcatt
    64981 ttgaccagcg accaggtgca ggtcgttttt gggcctggta aagcgcatcg tgccgcaaaa
    65041 gcgatgagcg agctgctggg cgatgcaccg gtacaggatg ccgcagaaat cgccgcccag
    65101 aacaaacgtc agttaaaagc caaacaaacc tccggcgtgc aacaatttct cgccaaattc
    65161 gccaccatct tcacgccgct gatccccggt tttattgccg ccggtctgct gctggggata
    65221 gcgacgttaa ttgccacggt gatgcacgtt ccggcagatg ctcagggcac gctacccgac
    65281 gcgctgaatt ttatgaaggt gttcagcaaa ggtttgttca ccttcctagt gattctggtg
    65341 ggctataacg ccgcccaggc atttggcggc acgggcgtca atggcgcaat tattgccgca
    65401 ctctttttgc tcggttataa ccccgctgca accaccggtt actacgccgg ttttcacgat
    65461 ttctttggtc tgcccatcga tccgcgcggc aatattatcg gcgtgctgat tgccgcgtgg
    65521 gcctgcgccc gcatttaagg catggtgcgc cgctttatgc cggacgatct cgacatgctg
    65581 ctgacctcgt taatcaccct gctgatcacc gctacgcttg cgtacctgat cattatgccg
    65641 ctgggtggct ggttattcga aggtatgtca tggctgttta tgcacctgaa cagtaatccg
    65701 ttcggttgtg cggttttagc cgggctgttc ctgatcgccg tggtatttgg cgtgcatcag
    65761 ggctttattc ctgtttacct cgcgttaatg gacagccagg gattcaacag cttatttccc
    65821 atcctttcaa tggcaggcgc gggccaggtg ggcgcggcgc tggcactcta ctggcgggcg
    65881 caaccgcaca gtgcgctacg cagtcaggta cgcggggcga ttattcccgg cctgctgggc
    65941 gttggtgaac cgctgattta cggtgtcacc ctgccccgca tgaagccgtt tgttaccgcc
    66001 tgtttaggcg gcgcggcggg cggtttgttt atcggattga tagcctggtg gggtctgccg
    66061 atgggcttaa acagcgcctt tgggccgtct ggtctggtgg cgctgccgct gatgacttcc
    66121 gcacaaggca tccttccggc aatggcggtt tatgctggcg ggattctggt ggcatgggtt
    66181 tgcgggttta ttttcaccac gctctttggc tgccgtaacg tcaatctgga ctgatattat
    66241 gaaacggaca atgctctatc tttctctgct ggctgttagt tgtagcgtca gcgccgcaaa
    66301 ataccctgtt ctgacagaag gtagttgatc aagtatatgc ggcgttaaag cagtaataaa
    66361 aaggccagtc agtaatgtta ctgcctggcc tttaatcaat gaatcagaaa cgcttacagc
    66421 gccatcaact tgtccagcga cggtgcgaaa taatagccgc cggttaccgg tttggtgaaa
    66481 cgcaacatcg catcacgttt gccatcggta tcgccaaaca tgctcagcag ttgctgctca
    66541 atgttatgca gacgcgcgca gtaggcgcag aagtacagac cgtgagtgcc actggcggtg
    66601 ccgtacggca ggctctggcg aacaatcttc agccctttgc catcttcttt cagatcaacg
    66661 cgggtgaggt gagaggtttc cggacgttcg tcgccgtcaa tctcttcgtt ggcctctttg
    66721 gtgcgcccga tcatcatctc ctgatcgtga acgctcatcc ggttgagctg cttcaggttg
    66781 tgctcccaac gctgcacaaa cacatagctg ccgccggaat ccacgccgtc tttgataacc
    66841 gccacttcgc ggcgcgtctc ttcacccgcc gggttttctg taccgtcaac aaagccgctc
    66901 aggtcacgct cttccaccca acggaagccg tggatctctt ctttcacttc aatgcagtca
    66961 ccaaaggctt ccatcgccgc ctgagcgaca gagaagttta cgtcgtgacg cagagaaaga
    67021 atgtggatca acacatcgaa ctgggtcgtc ggcgcaaggc ctttaccgta gcccggaaaa
    67081 tctttcagct cttctgcccc aacgccgccg ctcagagcgc gccaggtgtt gttaccaaag
    67141 gcaaccaccg caccaagatg cgcgtccggg aatttcgctt caaaagttgc cagtttgtcg
    67201 gcaaatgttt tactggccgc acgcagggcg tcaacttccc ctttcacgtt ggcttcgatc
    67261 caaatcgccg cgcggcaatg ttctggcaaa atgccactct gaacctgaga cattgttcct
    67321 cctgaaaata agaatgccac gcaatccgtg gcattgttga gcgctatttt acgtgttttt
    67381 tcgcgacggc ggtttgttca gacgcaaatt aacgccgcca gataatttta ctgactttcc
    67441 agtttttcag ggtatcgtcc gaaggcatta acccttcagg accacgccat tccccgctga
    67501 actggtagct aatatgttga ctgccttcgg ctttacattc gacggcacga ttatcatcac
    67561 catcagcttt ttggcaattg ccaaatgctt tgctgtaaag gtcgctaaac ggtgtaccga
    67621 ttttaacacc ggtgtcagca ggaatatcgc tatccagcac gtcaatgcga ctgatcgtgc
    67681 cttgatcgcc gttaatcacc atcgccacgt tgtcgccttt catcacttca aaaaagcgca
    67741 ccacgttgcc gttcgcggtt ttcattccgc tgcgcaggcg ataatcgcca tcaagcgcat
    67801 cagcaatggc ttgttcttgc agtggtgtgg acgccgttaa ttcacccacg ccctgctcgc
    67861 tcactttggt ggacgatcca aaccagttcc acgggttagc ggcagaccag ttaactgacg
    67921 acatcgtgga acagccggtc agcatcaacg gcatagcgca taacattaaa cgcagcgatt
    67981 tcatgtcact tcctttggtt attcaataac gttgcttgga gtgcaaattc accaaaaagt
    68041 gccgttattc tttttcttca ttaaaacaag cgcgtaaacg tcgattggtc agcagccaga
    68101 ttaacgccac aatatccgcc actaccagcg ccagaccgat accgctaacg gattcaccgt
    68161 tcagccacag ccacggttgc cagcaaagta aaaccacctg cgccaacagc aacagaaaat
    68221 agagcacatg ccaggtgcga ggaaacgaag cccgccgacc gctcaacaaa aacgccagca
    68281 ccgccggaat accgggaatc agccccagcc agaaattatc gtgatcggga taaaacagat
    68341 ttagcagcgc agtaccctgc tcacgcgacg caccggcaat gacaaacagc acccaggttc
    68401 gcgcctgaag caatagcaca agccagaaga gcaagggtaa acgcaggcgt ccgtgcgcat
    68461 cataatggac aggatggaac tcagtactct tcatcttcaa tcaaacgctt acccagactc
    68521 agcacgtcgg cgtgttcata tcccaggcgt tcatacattc cgagcaccat gtcgttatct
    68581 tccggcacgt tgatctgaat tttcgggcag ccgcgagcaa tcagcttttt ctccagccgg
    68641 ttaagcaacg cattggcaat tccacgccca cgaaactctg gatgcacgcc aagataataa
    68701 gcagacccgc gatgcccgtc ataaccgccc atcaccgttc cgaccacttc accgtttacc
    68761 tcagcgacca aaaacaaact gacgtcatgg ttcatcttac gctcgatgtc catttccgga
    68821 tcgttccacg gacgcagcaa gtcgcaacgc tcccaaaggg tgatgacctc ttcgaaatct
    68881 tcctggcgaa atacgcgtat ctccatggta ttcgttacct ttttgcgggt taaaaggctg
    68941 attatggcgt gaacggtcga attagccaat atctgacgaa aatcggttga aaaagtggca
    69001 taatggggag ttgtcaacta ttgaaatgaa aagtaaaaca attctcaaca gcaaaccgtc
    69061 gtaacggatt acgcgatacg atataacatc tggaacttta ttattacaac tcaggccgta
    69121 tgagcacttt taaaccacta aaaacactca cttcgcgccg ccaggtgctg aaagccggtt
    69181 tagctgccct gacgttgtca ggaatgtcgc aagccattgc caaagacgaa cctttaaaaa
    69241 ccagcaacgg acacagcaag ccgaaagcca aaaaatctgg cggcaaacgt gtcgttgttc
    69301 tcgatccagg ccacggcgga attgataccg gagcgatcgg acgcaacggt tcgaaagaaa
    69361 aacatgtggt gctggcgatt gctaaaaacg tccgttccat tttgcgtaat catgggattg
    69421 atgcgcgttt aacgcgttct ggcgatacgt ttatcccact ttacgatcgc gttgaaatcg
    69481 cccataaaca tggcgcagat ctgtttatgt caattcatgc cgatggcttt accaacccga
    69541 aagctgccgg tgcttcggta tttgccctct ctaaccgtgg ggcaagtagc gcaatggcga
    69601 aatacctgtc tgaacgcgaa aaccgcgccg atgaagttgc cggtaaaaag gcgactgaca
    69661 aggatcacct attgcagcaa gtactgtttg atctggtgca aaccgatact attaaaaaca
    69721 gcctgacgct tggctcgcat attctgaaaa agattaagcc ggtgcataaa ctgcacagcc
    69781 gcaacaccga acaagccgcg tttgtggtgt tgaaatcgcc gtcggttcct tcggtgctgg
    69841 tggaaacctc gtttatcacc aacccggaag aagagcggct gttaggcacg gcggcgtttc
    69901 gtcagaaaat cgccacagcg attgctgaag gcgtgatcag ttatttccac tggttcgaca
    69961 accagaaagc acattcgaaa aagcgataag ttatgaaacc cgacgcacac caggttaaac
    70021 agtttctgct caaccttcag gatacgattt gtcagcagct gaccgccgtc gatggcgcag
    70081 aatttgtcga agatagttgg cagcgcgaag ctggcggcgg cgggcgcagt cgggtgttgc
    70141 gtaatggtgg tgttttcgaa caggcgggcg tcaacttttc tcacgtccac ggtgaggcga
    70201 tgcctgcttc cgccaccgct catcgcccgg aacttgccgg gcgcagtttc gaggcgatgg
    70261 gcgtttcact ggtagtgcat ccgcataacc cgtatgttcc caccagccac gcgaatgtgc
    70321 gtttttttat tgccgaaaaa ccgggtgcag agcccgtttg gtggtttggc ggcggcttcg
    70381 atttaacccc tttctatggt tttgaagaag acgccattca ctggcaccgc accgcccgtg
    70441 acctgtgcca gccatttggt gaagacgttt atccccgtta caaaaagtgg tgcgacgatt
    70501 acttctacct caaacatcgc aacgaacagc gcggcattgg cgggctgttc tttgatgatc
    70561 tgaacacgcc agatttcgac cactgttttg cctttatgca ggcggtaggc aaaggctaca
    70621 ccgacgctta tttaccaatt gtagagcgac gtaaagcgat ggcctacggc gagcgcgagc
    70681 gcaattttca gctctaccgt cgcggtcgtt atgtcgagtt caatctggtc tgggatcgcg
    70741 gcacgctgtt tggcctgcaa actggcgggc gcaccgagtc gatcctgatg tcaatgccgc
    70801 cactggtacg ctgggagtat gattatcagc caaaagatgg cagcccagaa gcggcgttaa
    70861 gtgagtttat taaggtcagg gattgggtgt aactccctca cccccattcc cgcatccgct
    70921 gatgcaacgt caacgacggc ttctcggcaa acagctgctg gtaatccgtg gcaaattgcc
    70981 ccagatgcca gaatccccac tgcatggcgg cgtcttttac cgttgtgctt tgcgaccacg
    71041 ggcttatcag ttcgcggcgt acggcgttca ggcgaatgcg tttcagccac gcgtttgggc
    71101 caatgcctaa aatagcgtga aacgcgtttt gtagcgtgcg gcgactgaca tgcagttgat
    71161 tacacaaatc cagcaccgtc accggttcgg acatgttttc cagcacatat tcacgggcgc
    71221 gggaaagcaa ccgacggtaa ctctgatgac tgatgctttc cgccgtcacc attggttgcg
    71281 cttcttccag catcgccccc atcgccatta gcaaattatc ccccagcact tttcgcactg
    71341 caggctgatg gagattttcc gggttctcgc aaaacgtcgc cagcgcctgt tggacaaagc
    71401 cccacagcgc ggctttatgc tgctctttca cttccagcgc cgactggcta cgcagcatat
    71461 gtaatacccg atccggattg tgcaaaaagt tagcctgaca ggtgatgaca tcttcagaca
    71521 gcactacgcc caggatcgta taatcgtccg gcgtacttaa ttcaaactcg gttccaccgg
    71581 ggcgcgtcgc tatttctgcg cttcccagac attgcgaacc gataaatccc tgctcaccgc
    71641 gcgtcgccgg aatgccaaac cagaacgagt tcggccagac caggcacgac tgacgcagcg
    71701 ccagaccggt gtattcacga aaaacctgaa tatcatcgag tagaatttcc gtaaattcac
    71761 catgaaactt gcccggatgc agctgatcgt aaatctgctg ctaggcggta atcgtcaaag
    71821 catgttcata gacatccgtt gtctgtcgtt gatgaacatt atccacttcg accttcggcg
    71881 tgagcttcag gttttcgggt aagggttcat gataaagatg gtgcaaattg gctgtacggg
    71941 tctttttcat gatgttaatg ccgggtgttg taggacaccc ggcacctccg acaggttaat
    72001 ggggcttgag acgataacga ctactgcgtt tacgtaacgt cccggcagaa aagagctgtt
    72061 cgagcgcatt tctggctttt tccagcggcc agccaaagtg ggcggccact tctcctgccg
    72121 tcattccctg acatactgat gttaacagcg ccaacaactc gtcagcagat tccgcgacga
    72181 taactggcgc gtcgggttcc tttacgggtt gcttcggctg gcggttaaag ccagtaacca
    72241 gccactgggt gtcatcgtct ggccgaccga tctccttacg gctgatgaca cgcccggtac
    72301 gcatcgcggc agcacaacct gcgtccagcg ccgcacgaca cgccgccaga tcgccttcca
    72361 ccaccagcgt taagcgacca gggtcaagca cttcgtgact gagcagacga acgttagctg
    72421 ctttgagcat ggcatccgct gcatctatcg cggcgaccat gccgtccact tccagcaatc
    72481 ccagtgcatt gatcatcggc aacccccgtt acgcacgctg gattggatta cgcgcgattt
    72541 ccagcactgc atcggtaaag gcgttacagg ctgctttaca cgcggcctgg ctaccggtta
    72601 aaaatgctgc cgagtagttg gtttcagacg gtggcgggac gtaggtcacc agctgtacgt
    72661 cggcagattt caacgcggca tcaatgccgt aggtcgcttc cagcggcggt gccaccagat
    72721 acgccatcgg atcgccaagc gtgatcccgg cggttgatga aagataagaa ccggtacgcg
    72781 aaaccacatg cgccaggaag gcggtgtttt ctgcgtcatt ggcccactgg aaagctgcgc
    72841 cattttcaat atgtgcaacc atcgcatcca gaccggcacg cacttccgcc gggtttggcc
    72901 caccaagcat aatcagcact tcaccggcag tcggtgacgg gccgtgtgcc gctcccgcat
    72961 acagcgagcg accatatacc acttcgacca tcgcttgttt ggtcgcttcg tcagccgcaa
    73021 tgtaggttac gtcgtcagaa tcggcagaaa tcagcccgag gctacgaata tgcggcggca
    73081 attttagttc acgcgcaaat tcagcgttaa cggaggcaat cacccgcatg gcggttaccg
    73141 acggtcgaat caaatctaaa gctggcatga tgcctcctta tcgggtcatg ttgatgccgg
    73201 atgctttctg ctccagcata cgtttcgcca aatccacaat gacggcacag cagcacgaaa
    73261 gagaagcaaa agtcagctac ctggtacact aggcagcggg tcagcaaagg ggggcaaatg
    73321 cccctttttt catgtcagat aatcagatca ttacctcaat aaactaataa tcacatcgcg
    73381 gttacaacca gtttgatagt gatagctcaa acccagacgt ggcgctggct gcaagcaaaa
    73441 taagccgcaa aaatgcgacc ccatgtttac tagccgcaaa aatgcggtcc tatttgccag
    73501 aaccttttat tattcaattg gttagtgcaa gaaaaagcgt gattgctgtt caagaagtat
    73561 ccagaaaggt catgattttg acgctgaaaa aaaataggtg ggcatttttg cccactcact
    73621 agccgcaaat ttgcccactc actagccgca tttttgcggc ccaaagtaat aaatacagag
    73681 aaataataaa tacagatcag cgccaagatt tttcgtcttt tcggcggaat catgagacta
    73741 gtgcttacgt cctgataacc tccagagcag accttaactt ctgtgcagca ggactacgct
    73801 cattgccgct cggctaggtc aaatcctcac gataaagctg ttcggattgt gaccataatg
    73861 ttaaaggact ctttaaatca ttccttgacg aattaactag ctggcttaag cggaatagca
    73921 tgcaggataa aaacaaaacc agcttagcag tggggagagc ttcgcttcgc tcgtctcctg
    73981 gggccaatgc tcaatgaaac gacgtaaggc acggagcgct caccataggt agacacataa
    74041 acatctgaga gcgcaccttc tatcgcgcta cgcgctcaat aaaaaccaac gacaccgcca
    74101 cactgcaaga ccaatggttt ttatcgcgta tacagaccag acaggaaaca gcaacaaagc
    74161 agcgtatagc caggtgcaga cagcacactc acacgacgca cagcaggctt aagtgatcac
    74221 tcaggaaact ttatccccgc cgccgatcac cgcgaactat catgaccgcg ccaaattcga
    74281 aaggaatggc ccatagaacg cgatgaacac acataagcgc tacacttact taacgtcgcc
    74341 acagagcgat tgagagcgct taaaatcgat gcgatttcat taaatttttc tcactcgcct
    74401 gaatggtggc gatcaggact gatcgcaaca ttgccccgcc tcgatattga gcgcataata
    74461 gatacataca ttaagcggaa aagagataag tttattgtga aaatcagggg gttacatatc
    74521 atgcgcatat cagaacaaca acggcagatc ctcgactatc tggcaaaaaa cgaatcaatc
    74581 accgcacgac aagccgcaga tctcgtttat ggtggcaatg ttacccgcgt ccaggttgac
    74641 gccgcccgcc gctcactcat gacgatggtt agtaatgggc taatcaggaa acaaggccgc
    74701 gctttcgtag ctgaaagaca tatcaatcac gcttatagcc aggacgttga agaattgcgc
    74761 cgccagatcc tttgggtatt cagcaaacag ggtcaggagt atttcctttc tgaacaccca
    74821 aatttcgccg ccaaagagtt aacagtaagc ggattgtcgt gcgcactgga ttatcttgtc
    74881 ggtgaaggtt gcgttactta caagcagcta tggcacgccc tgaatacgct tgtagaagat
    74941 ggtttactca ttgccacgcg tgagaacatc gaatactgga taatgccgcg ttcaggtcgc
    75001 cgcaatcatg cacggtatat ttcaggcaac agatacagaa cgccggactt taatccgaca
    75061 caggaagatc gggaccgtgc cgaaatgatg tgcagggaac aggagaaaag aattatggat
    75121 aagttatttt cgatgcagtg aacacaagcg ggcgcaatgc ccgtttttat tgccttattc
    75181 cacgcctgac gaatcaattt gatcacacct gagaaaatgc atagccagaa gttaaggccg
    75241 ttatattgcg attgggagcg ttttaaacgg tatcaattta attaattctg atgtggtgat
    75301 ttgcaggcaa aaaaaacccg ccggagaagc gggaaataga gacatatcgt tgtagttatt
    75361 aacagatcac tttgaggtcg agggcggcta accctcgcta atattatccc gctattacct
    75421 tgcgggtgca aataatcttt tattgctttg atgatttaat aaatagcact ttttgctaac
    75481 aaaaactgct gtcaatatat ggccaaaaat gtcaacagaa aaaataaggt ttataatatt
    75541 aagtatcaca tgggtaatgg gttcagggaa tcggaatcca ccgcctcacg gaaattcaaa
    75601 atttgcttct tctttcctcc gtgggcggct tctttttcgt atcgaggtat ttatgaaagc
    75661 aataacatta gaacagttcg cagcaatggc gaatgtatcg accagaacgc tgaggcgctg
    75721 gctaaaaaac catcaaccgg agtccctttg ccatttaccc cacgcggtac tcgccaatta
    75781 ttctgggagc acgaatgcaa agaatggttg tacagaacag aacgataata atcacgcatc
    75841 acattttgag gtaaacatca tgaccaatat cgaaagatta gaacgctgtg ccgatattat
    75901 gcggcgcaga tggatttatg accctgaagc gggtttattg tttagccgtg aaacaaaaga
    75961 cgttatcaaa ggcagcatga ccaataaagg gcatttggtt gtaacggtac atgaaggtaa
    76021 attcaaagtg acactcacct atcagaaagc ctgctatgta tacacatacg gcgcttatga
    76081 cgagacatta tatgaagtgc accacgttaa tttgaacaaa caggataacc gcatcaagaa
    76141 tattcgcctg atgccgaaag aaaagcaccg ccggatacat aaaaacgtcc gcaaacttat
    76201 tcggctgggc cacacacaat ggcttcaacc gcaattaatt gaacaatcac tataaacaca
    76261 ggagtaaata ccatgcattc attagacagt tattttcagc gcacgacagc ccctaaatca
    76321 gccgcccagg aacgccgtga ggaattccat gagaaagtga tgcgttctgc tgattatatt
    76381 gcggacaagt tcgtggaaac ggttcgtcca cttgttgatg aagtagctga taaacttcag
    76441 tctgaaatgc cggaagatat ggaaggcact gcaaagcggc gactcatttg cgaattaagt
    76501 cgtcgttttg gcgtgtcaat aagcgctttt aaataatcga aaaaaggggg gaaagataat
    76561 ggacgcattt gaggtactgg caaataaagg cgctgaactg gtagcacagc gtgataaaac
    76621 cgccaacgaa ggggaacgcg cactgctaaa taaacagatt aaagcgatcc gtatggccca
    76681 gtttaaatta atcagtaatg aagttatcga aacaattaaa ccgcagatcc cacaaatcgt
    76741 tgctgacgct attaaagccg ccggactgga gaaacgcatt gccgggctaa aaacgaacgc
    76801 agagaaaatg gagatgcaca aacagagcgg tgctccgaat cccgatgagt acttctcacc
    76861 ggatgttgag tttatggcgc aggttgagga acgtctgggt agctgtctca ccgaggaaca
    76921 gcgtcgctat tttgatggag tggatagttc ggcaggtatt gacctgaact cgtatttcgg
    76981 tcgtgaaatt gagcattttg atgccgcccc gtctatgccg gagccagaac cagaaatggc
    77041 caccgatcct gaagctgagc aacggaaacg cgtattcaat gcgataggga ttcggtactg
    77101 atcggataaa agaaagccgg gaattacccc ggctgactta acaatgtgac caatgaacaa
    77161 atcggacaag acaaacagga taagcagtct cgaaacaatg ttcgggaact atattagcac
    77221 aagtccggag aaaatcatca tgttggcact acgcatcatg cagggcatcg ctaagaccct
    77281 cgcagaacat gtcttagacc tgaaacattc gccactgagc aaacaggcca tgaagcgcca
    77341 aacgcttcgt ctgtgggctg aatattcgct tggaactatc aataaaatca tcgacatgaa
    77401 atcggggccg agcaaccaga gcgcggagga aatggagttc atcaggcgtt taattctcat
    77461 tcgcagagac attcatagcc agttacacag cgtgggaatc gatattaatg atgggacggg
    77521 cgattaattt tcggacaagt actatgcgcg cgcgaggagc tgaacatgaa gcaagatatt
    77581 gcagacaggc tggagatact cgaaggccag cgggcagaag cgaaacaact acgtaagcag
    77641 gccagaagag cgcacaggaa caacgaagca gagttactaa ccaaatacat cagtttcacc
    77701 aattactgca tctacgagtg ttacaaggaa gatgcagagg actggctgga ttcattgcca
    77761 gaacagtact gagcagaata acaccacgtc atgttgttct gaggtgagca tatcacctct
    77821 gttcaggtgg ccaaatttag tgtgccactt cagcatcacc gttgaaatca caataacgat
    77881 ttttttgtaa atatgctctc acatatgtag gaatagacca ggttggcatt gaaacacatc
    77941 taccatgacc ataataaagt aattttttga tatgtttatg gttttatatt aattaaaata
    78001 tatctaaaat tgatagtttt aaaattgcaa aatccagatt gtgtttgtaa tgtttaatct
    78061 gattgtattg agtggcgttt attatgaaaa ataaatcagg taattcttgt tatagagaag
    78121 cagtcgatat tcatagctca cttgagcttt catttatatt gcataaatat catttgcgtg
    78181 tagatgagaa gaaatctcga aatgtattca gcgccatacg tgatgagctc atccttgatg
    78241 gggattcacg acaaaactta gctactttct gtcaaacatg gcttgatgat gaaatccgtg
    78301 agttgcttaa tttagctatt gataaaaaca tgatttataa agatgaatat ccacaaatgg
    78361 cggaacctga aggtcgctgt gtgcgtatgc tttccgattc gtggagtttc cccgattcgc
    78421 gacacacctt aggttgctca accattggtt catctgaagc tgctatgctc ggccgcttgg
    78481 cgttaaaatg gcagtggtgt aaaaaacgcg aagtacaagg taaatcaacc gaacctgatt
    78541 tgcgatcctg tgcaaatatg ctggcataaa ttctgccgtt actgtgatgt gaaattgtgt
    78601 gaaattccac ttgatgatca tcacctgata atgaatgcta aagaggtttt gaagcatgtc
    78661 gatgaaaaca ctattggtgt cgttacgact ttaggtatta cttatttttg taaagcagat
    78721 tgcgttagtg tttttaagat aagtgttaaa gttaatatta gtttttatat ttacatcgta
    78781 attatatgtt tctattttaa aaatgttcga gagtataaaa atgtctaatg ctaatatcgc
    78841 acccaaaagt aagaaaatga cagtggggac actcgctatc atgaatataa ctgctgtagt
    78901 aagtctacga tttatacctt ctgaagcaga atacggctta ggtgccattt tttactatac
    78961 ctttgctgca attatgtttt tagtacctgt tgcctttgtt gcggctgagc tagctacaac
    79021 atatccacaa aaaggggggg catttcgatg gataggagaa gcatttgggg ctcgctgggg
    79081 ttttgtagga atgcttatga cctggctaca agtcatcccc tactttccaa cagtactgac
    79141 atttggtgca gtctcggttg catttattga tgttgatatg ggggtggcag aatcaattgc
    79201 cagtaataaa tggtatattt tatttttcgt actatttgtt tactggggag ctatttgtgt
    79261 tgcattacgt ggcgttggta ttttctcgca ggtatctaag tggtgcggta ttatagggac
    79321 aattattcca gctattatcc ttgttattct tggtttctcc tatctgtggt tcagcggtaa
    79381 accagcggtg atcccattag gttggggtga tcttttccct aatatagcta acttccagaa
    79441 tattgttctt gccgccagca tatttcttgc ttatgcaggg atggaaatga atgcggtcca
    79501 tgttaacgat ttagataatc caactaaaaa atacccaatt gcaattacca tcgcttctat
    79561 aggtactatt gcaatattcc tgctcagtac tttaggggtc gcctttatta ttcctacaga
    79621 taaaattagt ctaactcaaa gtttgttggt tgcctacgat atgctgttcg aatgggctgg
    79681 agtgccatgg cttggctctg tgatggcatt tatgctagca attggcgtac tgggtggggt
    79741 ggttacttgg attgcagggc ctaatacagg tgtattagcg atcgcgaaag ctggttattt
    79801 acctaagttt ttccaaaaaa ccaatcgtca tggaatgggg caccatctaa tgtttgtaca
    79861 aggtataatt gtctcagttc tttctgttac ttttgtgata atgccttcag ttcaagcagc
    79921 ttttcagatt ttatcacagt taacagttat gctctattta gttatgtata tgctcatgtt
    79981 cgctagtgct atctatctac gacattctca acctgataca aaacgcccat atcgagcccc
    80041 tgctttattt ttctgggcaa cacttggatt cttagggtct tttctggctt ttgtattaag
    80101 ttttattccg cccgagcaaa tcccaactgg tagtccaacc agctatgttc tttatttgtt
    80161 agtattagtt gccctatttt tattaattcc aatattaatt tacgccttcc gaaaatctga
    80221 ctggaaagat ccaaactctg atttcgaacc attttcttgg gaaaaaagaa ctagtaatta
    80281 aattttccat aagctacaaa agatgaggtt aaatatgttt agtgctgaat ctccaattgg
    80341 tgtaatagac tcaggtgtag gtggattgac atcggtaaag gaaataatta atttgcttcc
    80401 tggtgaagat attatctatt gtggagataa tatgaatgca ccttatggca accgttctgc
    80461 cgatgatatc atcagtttga ctaaaaaaat gctgactttt ttacagtcaa ggaatgtcaa
    80521 actagttgct gtcgcatgta atactatatc ctcaactttg gaatccgaag aatacagcgg
    80581 atatgctaag agctttccgt ttcctatctt atccatcatt gaacctgcag tagaagatgt
    80641 gattcgccag caatataaaa atgttggtat tatcgctacc gagtttacta ttaaaacggg
    80701 gtgtcacaaa gagctaataa aaaaattgaa ttcaacaata aatgtatttg gagaaccaag
    80761 caaaaatctt gcaatgttaa ttgaggaagg taatcttaat gctcctgcaa tcttgaatga
    80821 catcaaaaaa catgttaacc atttaatttc cctccatcca gtaaatgaaa ttattctcgg
    80881 ctgtactcac tatccaattg ttcagaattt ttttgaagca gttgcaccgg atataaaatt
    80941 tattaatcct gcacatgatc aggctatctc tattaaaaat cacttgggac agctaaattt
    81001 attaaactcc tcaaatatag gaactttgca aataaatact agtggaagta tggagatcta
    81061 caaaacggta ctaggggaat tgagcattac gaaacctcat acattcagta tcaggcagtt
    81121 ttaaatttat ccggtgcacc gtaaaatccc gtccttctga actgcacccc aaaagttgga
    81181 caccaactga gtaaggtgca ttttatggac aaacccagtt acatactggg tagtgcatcc
    81241 aattagtaga acatgtgttt ttcgataaac gctccgatca ctttttcgtg gatctcaact
    81301 gagcgtgaga agcagattgt tttacgaccc aaccgcttaa tgcgggtgcg tagcgtcaga
    81361 ttattacgct caatgcgttg ggtgaatatt ttgccggtca gatgcttatt cttcggcacc
    81421 tcccggccat agctgcccca gtcatcgctg gtcagcatgc cgatgttgaa gggtgtaagc
    81481 agtgccagta gctcccggca cgtttgatcg gttcggggac caaaagtgta ggccagtaca
    81541 ccgcctgttt tggtgttgta cgcgtacccg agccaatgtt gccgggcttt actgccaacg
    81601 tagctccatt gctcatcaag ctcgcagata aacgccacat cagcatgggc aacaggcgaa
    81661 gaagttattc gctttggcgc gagtttttta aagtccggat gacggtgtta ctaccaattt
    81721 tcagtgtcct ggcggtatcg cgaaccccgg caccattaaa ggccatttca gttatcagct
    81781 ctttcatacc cggcttacgt gcttgataag tataagtgaa ctgaaacaca cggtggcagt
    81841 cacggcagcg aagtctgtca cggcctttag ggttctgacc atggcggtaa acctgagctg
    81901 actgacaacg gggacaatga atattaacgc tggtcatgag ataacctcaa aagcccgtat
    81961 tatacatcag attcaactaa ttagaggcat cacccatcca gcattataaa acatactaac
    82021 aataactaaa ttataataat cacaacttac aatcaaataa taaatgcata agattaacaa
    82081 tgattttttc gggttatatt tatctaaaaa agcagaatat tatgtaactt attattttct
    82141 aagcgaaaat ttattgtgcg gtcagactaa caaacttgcg taccacccga agaacatgcg
    82201 aagatttaag tattctttct tttttggttt ttatttttca ttttctcttt tcttctcttt
    82261 ccggttctgt tctggttttg gttttttcct ttttattttt attctttctt cagggttatg
    82321 ctaaaacacc aaaacatttt tcaccacaaa agcatccaat catcttgcca gaccaatcac
    82381 cttaaccgga tacactgccg gatataatcc cactaacacc cagacaccaa gcaccatacc
    82441 tgataactac cgtattcagg aaggaaatgc gagtgatttc accgtcaccg gcacaaccga
    82501 tccgccaaaa agaggcgtac caatgtcgat atagtccccc gcgcggacaa tcacttcgtc
    82561 aatgactgcc aacgggagtt gttgtagctg tgggcgcaac aacatgccca gagctttacc
    82621 aaagtcctgc ccggccacca ccagcagggg atgcggattc ggaaaacgcg cgacgaaatc
    82681 gaccagcgcg ttgatgaccg tcagaaccgc ggcgtaacgc acaggcagcg aggcgggaag
    82741 cgccagcacg tacacgtcag ttttgggatc aagatccagc tgaagcagcg cctgttgcca
    82801 ggcactcacc agatccgttt catcaatcgg gatcgccacc ggcaaattgc gcagcggcag
    82861 ttgtacgccc tccagccaga ttgtgctgcc agagagcgaa agggtatgtg cacccgcgcc
    82921 aatcaccgtg gagcgtacgg tttgcgccgg aaactgcaca ttcatctcac gcaggcgcgg
    82981 atggtcatgc agcgccgttg ccagaagtgg gccaatatcg gcaaaacaga acgggttggc
    83041 gggctggtgg cgataacatt cgcccacgcc tccagaaagc gtaatgattt cgggcgtaac
    83101 acctgcgggc agcaaaccgg tttgcatcaa tgcctgcgcg agcggcgaaa gcgttccgtc
    83161 aatcacttcg acaatcagcg ccgccatccg ccgggtaacc tgcaccagct gcgcgccggt
    83221 cagcgaacgg gcgtcagtgc ctgcaccgaa gcactcatcc acaatcatct gccccggttt
    83281 atgagcgtaa accacgcgcc cctggctgtc ggtttccagc aggcgaccac cgacgttgag
    83341 acaggcagtg ccgctgattt ttccggcatc gaacagggcg tagttcgcgg tgccaccgcc
    83401 gatatcgata ttcagtaccc gacacagccg ttgttcagaa agggtttgcg ccccggctcc
    83461 gtgaccggcg atcacggatt cgaggtgcgg cccggcgctg gcgacgacaa aatcgcccag
    83521 cgactgtttt tccttcatca ctttttccat cagctgtgcg accaggcttt cggtaaactg
    83581 gccttccggc agctgggcaa tgatggtttc gcggatgcgc tggctttcgg ttttgtcggc
    83641 tggcacgctg gcgggcacgg acgccgttac cgggatcgat tcatcacatt cggtgatggt
    83701 aaaacccagt agatccgcca cttcacgggc ttccggggta ataatgctgg cgcgcagaac
    83761 cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga atatcattcg ctgtgatgag
    83821 ttttttcacc tgcttcctcc tgtttggtga gagatgccag ataatccacc agttgctgca
    83881 cgctttgcgg gtcgtggctg tttagctcga aaatcggctc ttcgaaaccc gtctcaagca
    83941 ataactttcg cgttgcggcg acgtcagcat ctggcatgtc cgttttactg atgacggcga
    84001 tttgccgttt gctgacgcca atatccagca acccggcagg caaccgactt tccgggtcat
    84061 ttgcgccatg gacatagatc agcatgtcta catcctgtag cgtggtaatt aaggcgtgat
    84121 accagcgggg atggctaaaa tattcacccg gcgtatcaat atcgccctta tcattaaatt
    84181 ccacggccag tgtttttctg gcgagggtat aattcccctg caacgcatta aataacgttg
    84241 tttttcctgc cccgacgcta cccacaaacg caattcgttt catgaatgcc tcgtattaac
    84301 ttttggtcat ttcacaaagc gtataattta ataagcgacc taaaccgctg acggtttgtg
    84361 ataacgcttc ttctactgcg cccaccgagc cataaatcac caatgcaccg ctgaagcggt
    84421 cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa atcaccggcg atcatcgccg
    84481 tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc atccggaacg ccgattttct
    84541 tcgccagttc ctcaccggga tgcgcgatga gatgcgccag tgtgacctgt ttgccgggca
    84601 caaattcctg aatgatgcgt tctttatcca tgagtcgccg cctgtgaaaa actttcgtgt
    84661 atcgtgacaa tttttcgtca gagtaaataa caaaattcgg caagcgcgtt taaaaggtga
    84721 ggtagatcaa taagaatgga gagatattta ctgttatgaa taaactttca tatgcgacgc
    84781 atgaatataa ataaaataat atcgagatat tttatatact gattttttta ttattcgcaa
    84841 actcagtaat aagctttgct aatatatttc aggaaaaccg cttaccctga atattcaggg
    84901 taagcgtgag agttaagaat gaattacagc ggttgggttt gcgcttcaac cacggccagc
    84961 gccaccatat tgacgatacg acgcaccgat gcgatcggcg ttaacacgtg aaccggtttc
    85021 gccacgccca tcagcaccgg accgacagtc acaccttccg agctggaaac gcgcagtaag
    85081 ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca gaatattggc agaacctttc
    85141 aaagggctgt ccggcatacg gtcgttgcga atcgcttcca ccagcgctgc atcgccgtgc
    85201 atttcaccat caatcatcag atctggcgca cgatccctga ccagttccag cgcctgacgc
    85261 attttgctcg acgacgggca gtcagaagaa ccaaagttgg agtgcgacaa cagagcaacg
    85321 cgcggctcaa taccaaaacg acggacagtt tctgccgcca tcaaggtgat ctccgccagc
    85381 tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa taaaggtgtt accactcggc
    85441 agcagcagcg cgttcattgc acctgcggtg tgaacgccat cgcgataacc aaagacattt
    85501 ttcaccacgc taaaatgttc atgataatcg cccaccgtac cgcagatcat tgcatcggct
    85561 tccccacgct gaaccatgat cgcgccgatc actgtcgggt tactgatcag cgcacgctgc
    85621 gcctgttcct gagtgacacc gcgacgcttc atgatctgga agtattcggt ccagtactct
    85681 ttaaagcgcg gatcggattc gttattgacg atttcaaaat caacgcccgc tttgatctgc
    85741 aagcccagtt tctgaatgcg catttcgatc acgttcggac gaccgataag gatcggtttc
    85801 gccagtccca gcgttaccag ttcctgagtc gcatgcagaa cgcgcgcctc ttccccttcc
    85861 ggcagaacta cgcgcttcgg cgctttgcga gcctgggaga aaatcggctt cataaacagg
    85921 ttggttttgt aaacgaactc agtcagcttg tcgatgtaga cgtcgaaatc agcaatcgga
    85981 cgcgtcgcca cgcccgactc catcgccgct tttgcgaccg caggagcgat cttaacgatc
    86041 aagcgcggat caaacggttt tggaatgatg tattccggac caaagctcag atcctgaacg
    86101 ccatacgctg aagccaccac ttcactctgt tctgcatggg ccagttctgc aatcgcgcgt
    86161 accgccgcca gtttcatctc ttcgttgatg gcggttgcgc caacgtccag cgcgccacgg
    86221 aagatgaacg ggaagcaaag aacgttgttc acctggttcg ggtagtcgga acggccggta
    86281 cagatgatgg catccggacg cacttctttc gccagcggcg gcagaatttc cggttccggg
    86341 ttcgccagcg ccaggatcat tggtgcccga gccattttct tcaccatttc ctgggtcagc
    86401 actttcgggc cggaacagcc caggaaaata tccgcacctt caatcacatc atcgagggta
    86461 cgtttgccgt catccaccac cgcatatgcg gctttggttt ccgccatatt tggctcacgg
    86521 ccctgataga taacgccttt tgaatcgcaa accacgatgt tatgtttttg cagccccagc
    86581 gctaccagca ggttcataca ggcgattgct gcggcacccg cgccggaaac caccatccgc
    86641 acgtcggaga tgtttttctc caccacgcgc aagccgttga ggatggcggc agtgctgatg
    86701 attgccgtgc cgtgctgatc gtcgtggaat accggaatat tcatccgctc gcgcagtttc
    86761 tgttcaatat agaaacattc tggcgcttta atgtcttcga ggttgatgcc gccgaaggtc
    86821 ggttcgagcg cggcgacgac ttcaataaat ttgtccgggt cgagttcatc aacttcaatg
    86881 tcaaatacat caatcccagc gaatttctta aatagaacgc ccttgccttc catcaccggt
    86941 ttgcctgcca gcgcgccaat gttgcctaac cccagcaccg ccgtaccgtt agagatcacc
    87001 gcaaccaggt taccgcgcgc ggtatatttg taggctttta gcgggtcttt ttcgatttca
    87061 agacaaggtg cggcaacgcc tggtgagtag gccagcgcca gatcgcgctg cgttgccaga
    87121 ggcttggttg gagaaacctg gattttccct ggaactggaa attcatggaa atcaagtgca
    87181 ctttgtttta actggtcatc catttggttg ttcctttcac gtaacgttca caaataaagt
    87241 gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta ttaccctgcc ggagtctaca
    87301 gactttgagc aagtccaaac tctcaccatt aatataatgt tttggtaata atcctataac
    87361 actgatgtta cctgcttaat ccagcaatac catgcctgtc tgctatgctt ttttgatgcg
    87421 tttagcgaaa tttctcagaa gtgtgaatta acgcactcat ctaacacttt acttttcaag
    87481 gagtatttcc tatgaacgag ttagacggca tcaaacagtt caccactgtc gtggcagaca
    87541 gctgcgatat tgagtccatt cgccattatc atccccagga tgccaccact aatccttcgc
    87601 tgttactcaa ggctgccgga ttatcacaat atgagcattt aatagacgat gctatcgcct
    87661 ggggtaaaaa aaatggcaag acccaggaac aacaggtggt cgcagcgtgt gacaaactgg
    87721 cggtcaattt cggtgctgaa attctcaaaa tcgtacccgg ttgcgtgtca acagaagttg
    87781 atgcacgcct ctcttttgat aaagaaaaga gtattgagaa ggcgcgccat ctggtggact
    87841 tgtatcagca acaaggcgtt gagaaatcac gcattttgat caaactggct tcgacctggg
    87901 aaggaattcg cgcggccgaa gagctggaaa aagaaggtat taactgcaac ctgacgctgc
    87961 caggcgcggg cctgtgcgga agcaggcgtt tttctgattt cgccgtttgt agggcgtatt
    88021 tatgactggt atcaggcacg caagccgatg gacccgtatg tggtggaaga agatccgggc
    88081 gttaaatcgg tgcgcaatat ctacgactac tataagcaac accactatga aaccattgtg
    88141 atgggcgcga gcttccgtcg caccgaacaa atcctcgcct taaccggctg cgatcgactg
    88201 actatcgcac cgaatttgct gaaggagctg caggaaaaag tttctccagt ggtacgtaaa
    88261 ttaatcccgc cttctcagac gttcccacgc ccagcaccca tgagcgaagc ggagttccgt
    88321 tgggagcaca atcaggatgc gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc
    88381 gctgttgatc aacgcaaact ggaagatctt cttgccgcca aactataaac cagccacgga
    88441 gtgttatatg tcccgaaaag accttgccaa tgcgattcgc gcactcagta tggatgcggt
    88501 acaaaaagcc aattctggtc atcccggcgc gccgatgggc atggctgata ttgccgaagt
    88561 gctgtggaac gattttctta aacataatcc taccgaccca acctggtatg atcgcgaccg
    88621 ctttattctt tccaacggtc acgcgtcgat gctgctctac agtttgctac atctgaccgg
    88681 ttacgacctg ccgctggaag aactgaagaa cttccgtcag ttgcattcga aaaccccagg
    88741 ccacccggag attggctata cgccaggcgt tgaaaccacc accggcccgc ttggacaagg
    88801 tttggcgaac gccgtcgggc tggcgatagc agagcgtaca ctggcggcgc agtttaacca
    88861 gccagaccat gagatcgtcg atcacttcac ctatgtgttt atgggcgacg gctgcctgat
    88921 ggaaggtatt tcccacgaag tctgttcgct ggcaggcacg ctgggactag gcaagctgat
    88981 tggtttttac gatcacaacg gtatttccat cgacggtgaa acagaaggct ggtttaccga
    89041 cgatacggca aaacgttttg aagcctatca ctggcatgtg atccatgaaa tcgacggtca
    89101 cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc
    89161 gctgattatc tgccgtacgg tgattggctt tggttcgccg aataaagcag gtaaggaaga
    89221 ggcgcacggc gcaccactgg gggaagaaga agtggcgctg gcacggcaaa aactgggctg
    89281 gcaccatccg ccatttgaga tccctaaaga gatttatcac gcctgggatg cccgtgaaaa
    89341 aggcgaaaaa gcgcagcaga gctggaatga gaagtttgcc gcctataaaa aggctcatcc
    89401 gcaactggca gaagagtttc cccgacggat gagcggtggt ttaccgaagg actgggagaa
    89461 aacgactcag aaatatatca atgagttaca ggcaaatccg gcgaaaatcg ctacccgtaa
    89521 ggcttcgcaa aatacgctta acgcttacgg gccgatgctg cctgagttgc tcggcggttc
    89581 ggcggatctg gctcccagca acctgaccat ctggaaaggt tctgtttcgc tgaaggaaga
    89641 tccggcgggc aactacattc actacggggt gcgtgaattt ggcatgaccg ctatcgccaa
    89701 cggcatcgcg caccacggcg gctttgtgcc gtataccgcg acgttcctga tgtttgttga
    89761 atacgcccgt aacgccgcgc ggatggcggc actgatgaaa gcgcggcaga ttatggttta
    89821 tacccacgac tcaattggcc tgggcgaaga tggtccgacg caccaggctg ttgagcaact
    89881 ggccagcctg cgcttaacgc caaatttcag cacctggcga ccgtgcgatc agatggaagc
    89941 ggcggtgggc tggaagctgg cggttgagcg ccacaacgga ccgacggcac tgatcctctc
    90001 aaggcagaat ctggcccagg tggaacgtac gccggatcag gttaaagaga ttgctcgtgg
    90061 cggttatgtg ctgaaagaca gcggcggtaa gccagatatt attctgattg ccaccggttc
    90121 agagatggaa attaccctgc aagcggcaga gaaattagca ggagaaggtc gcaatgtacg
    90181 cgtagtttcc ctgccctcga ccgatatttt cgacgcccag gatgaggaat atcgggagtc
    90241 ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa gcaggtattg ccgattactg
    90301 gtacaagtat gttggtctga aaggggcaat tgtcgggatg acgggttacg gggaatctgc
    90361 tccggcggat aagctgttcc cgttctttgg ctttaccgcc gagaatattg tggcaaaagc
    90421 gcataaggtg ctgggagtga aaggtgcctg atggtgatcg ccggatgctg tatgttcccc
    90481 tcaccctaac cctctcccca gaagggcgag gggacggtcc gtgcgctttt tagggccgca
    90541 tctgcgattt tgtttaacgg gtgatccaca acgtgggcct ggcgtctggc ccatgccagt
    90601 tatcgcaggt aggttctgcg gcgtaacgca ccaggcgaaa acgctgaccg tcaaagcgcc
    90661 agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc accagttcac
    90721 gcgatttctc atcaaatgtt gcgttcatca gttccagttc attcgtctcc tggccgttgt
    90781 tgaacggcaa acgcaaccga accgggcgcg aggctagcgg ctttttacgc gacacaatcc
    90841 atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc agtgctttat
    90901 catcggtcag cgcagtgaca ttcacctcac gacgtaacgg atcaagcgag cagcgcagac
    90961 cattcatccg ccagtttcca taatccagca aatcgttacg ttcttcgagt gagagtggcg
    91021 tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt acgctgagag
    91081 gcggttcgtc ccctttcttg atccacgcgg tttcactgcc aacgcgcttt tgctgagcat
    91141 caataaacaa caacgccgct tttaagccgc tcagagaaat ggtctgattg ccgtcgcgta
    91201 aggtgatcgc cttcccttcc tgaattatct gcaaaaacgc ggtgatggtt gccgtatcat
    91261 cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaatgcc aacggctcac
    91321 catctaatag cagccgtggc gctatctccc cttcagacgc atccggcgac ttcaacccac
    91381 cgcgctcaat acgtaaaacg gcatcggtat gcgccccagc gctgcggctc agggtcatca
    91441 ccagtccatt atgatcgccc gtattgcgcg ccacacaaaa attttgatta ttacaggtga
    91501 cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac gtcggcaaca
    91561 aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc ccagaagttc
    91621 aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat cggatttgtc
    91681 ggattgagcc aataataaac aacgaaatat tattgctatt tttcagtcca ttaaatgtga
    91741 catttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc cggttttgat
    91801 catctccagc gcctggctga acggcagctc gagcacttca atatcttcat cttcaacacc
    91861 gccaccggcg ttagcgcgct gattgtcact gtattcggcg ataaaaaagt ggattagctc
    91921 agtcacaccg ccaggcgaca tatacagttc aaataatttg cgcacttcgc ccacttcata
    91981 gcccgtctct tcaatcgctt ctttacgaat gcacacttcc ggttcgtcgt tatccagcag
    92041 cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg tagcgacacg
    92101 gaactgacga atcagaacca cgctcttttt cttcgcgttg tacaggagga tcgtcgcacc
    92161 attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt tgcgcgtgag
    92221 atcgtaagta atgttgtgca gggtgaaata gttatcggag agaattttgt ctttaatgag
    92281 ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattata ttacgctgtg
    92341 aagtcgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc cccagccagt
    92401 caataatccc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc agatccgcac
    92461 cacgcacggc gtcgccaccg gcgaagattt tcggattggt ggtctggtaa cggtactggc
    92521 tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc gactccagcc
    92581 acggcatccc gtgtggattg aagccaaacg ccataatcac cgcatcggct ggcatgacaa
    92641 attcactgcc ctcaatcggt acaggtcgcc gacgcccctg ggcatccggc tcgccaagac
    92701 gcgtgcgcag gaaacgaatt ccgcaaacgt gaccttgctc attcagctca agcgccaccg
    92761 gctggacgtt aaattcgaag ttggccccct cttcgcgggc gttcttcacc tctttcttcg
    92821 agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc gcgccgtggc
    92881 gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa
    92941 gtccggcggt attgataaac ggctcttccg gtagctcttc gagccccatc acctgtttag
    93001 tgttggcaat gaggaacggc agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac
    93061 ccgctttcat ggaacggtaa gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa
    93121 gcaaatccaa agagacatct ttacccactt cacaattgag ttcgaagtga atccccatcg
    93181 cgctgaagat ttcccgacgg cgtgccagca gggatttatc cagtttgaaa gaaggaatgc
    93241 cgaaagtgag caagccaccg atttctggat ggcgatcgta caccgtcacc gccacgccat
    93301 tgcgggtcag aacatccgca caggccagcc ctgccggacc tgccccgata atcgccaccc
    93361 gcttgtccac tttggtgaca tgacttaagt caggacgcca acctttcgcc aacgcctgat
    93421 ctgaaatata gcgttcaatg ttgccgatag ttaccgcgcc gtgctcatcg cgaatagtac
    93481 aggcaccttc acacaaacgg tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt
    93541 tggtctggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc agttcaatcc
    93601 actgcggaat atggttatgc agcgggcagg tccattcaca aacgctgtgc tcgccgaact
    93661 taaggcagcg cgaggcttcc cgttgtgcct ggtcggcgcg aaatggcaga taaatttcat
    93721 caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg
    93781 cctgcatttg ttcgacttta ctcattaccg gcatttcttg tgcagcggta ctggcatgcc
    93841 acggttgatg ctcctgacgt gcggtgcgca agcggcggga tttcgccatg ccggagagtg
    93901 cgacgtcagt gaccagttgc agcgcgtccg ccgggcaatt ctcgacacag gcaggaccgt
    93961 tttcgcgccc tgcacaaagg tcgcatttat gcgccgtggc ttttactttt cctgccgcga
    94021 cgggtgtcag gacgatttgc atcgtaccaa acggacaggc caccacgcag gatttacagc
    94081 caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt gcgccattag
    94141 ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcactgca ctacgttgct
    94201 gctgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg acatgttgct
    94261 catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga cattgctgac
    94321 tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa aaccttattc
    94381 tttatatgag tgttgttatt acccgactta caggggaacc ggcaatgttc atgtgcccag
    94441 aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca ctgactaaat
    94501 tgcgtttcag cgaactggaa cattaatgat tttttgtgga gaagacgcgt gtgattgtta
    94561 aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg ctgtcgattc
    94621 tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct gaggctatca
    94681 atattgccgg atcgctgcgt atgcagagtt accgcctggg ctacgacttg caaagtggca
    94741 gtccacaact caatgcacat cgccagttgt ttcagcaggc actgcattca ccggtattaa
    94801 ccaacctcaa cgtctggtat gtgccagaag cagtaaaaac tcgctatgcg catctgaatg
    94861 ccaactggct ggagatgaat aatcggctca gcaagggcga tttgccgtgg tatcaggcca
    94921 atattaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag cactacgctg
    94981 aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggtatcggt attttcacgc
    95041 tggtcttttt tactttgcgc cgcatacgcc atcaggtggt tgccccgctg aatcagctgg
    95101 ttaccgccag tcagcgtatt gaacacgggc agttcgactc gccgccgctg gataccagtc
    95161 tgccgaatga gcttggtctg cttgcaaaaa cctttaacca aatgtcgagc gagctgcata
    95221 aattgtacct ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc cacgagacca
    95281 agcgtcgtct ggaggtgttg tatcagtgca gtgttcccag gcgctgaaca ccagccagat
    95341 tgatgtgcat tgtttccgcc atattttgca gattgttcgc gacaatgaag cagctgaata
    95401 tctggagctg aacgttgggg ataactggcg gattagcgaa gggaagccaa accctgagct
    95461 gccaatgcag attttaccgg tgacaatgca agagacggtt tacggcgaac tgcactggca
    95521 aaatagtcac gtttcatcat cagaaccgct gcttaacagt gtttcatcga tgctgggacg
    95581 cggtttgtac tttaatcagg cgcagaagca ttttcagcag ttattgttga tggaagaacg
    95641 tgcgaccatc gcccgcgaat tgcacgactc gctggctcag gtgctttctt acttacgtat
    95701 ccagttgacg ttactgaagc gttcgatacc ggaagataac gccaccgcac aaagtatcat
    95761 ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag ttacgcgagc tgttgactac
    95821 tttccgcctg acgctgcagc aggcggatct cccctccgcg ttgagggaaa tgctggatac
    95881 gttacaaaat caaaccagcg ctaaactgac cctcgactgc cgtctgccaa ccctggcact
    95941 ggatgcgcaa atgcaggtgc atttgttgca aattattcgc gaagcggtgc tgaatgcgat
    96001 gaagcacgcc aacgccagcg aaatcgccgt cagttgcgtc accgcgccgg acggcaatca
    96061 cacggtttat atccgtgata acgggattgg tatcggtgaa ccgaaagaac ccgaaggtca
    96121 ttatggactg aatatcatgc gcgaacgcgc ggaacggctg ggcgggacgc tgactttttc
    96181 acaaccttcc ggcggcggca cgttagtgag tattagcttt cgctccgcag agggtgaggg
    96241 gaaggggaac acgccggctg aggtgtaaat aattaccctc acaccggccc tctcccttta
    96301 aaggcgagag ggaaaaacgt gcccatattg acctgtaagc gagtgtgtca gcaatctcat
    96361 gataatttac attaactcct ttttttctcc acgattggct cgtaccttgc cgctacagtg
    96421 aagcaagtca agcctacaac aatacgcaga aacacgaggt cctcttttaa tggcgaattt
    96481 ctttattgat cgccccattt ttgcctgggt gctggcaatc ctgttgtgtc tgacaggtac
    96541 cctggcgatt ttttcattgc ccgttgaaca ataccccgat ctcgcgccac cgaatgtgcg
    96601 agtgaccgct aactatcccg gcgcatcggc ccagacgctg gaaaacaccg tgacccaggt
    96661 tatcgagcaa aatatgaccg gcctcgataa tctgatgtat atgtcatctc agagcagtgg
    96721 caccggtcag gcatctgtca ctttaagttt taaagcaggc accgatccgg acgaagccgt
    96781 gcagcaagta caaaaccagc tgcaatcagc catgcgaaag ttaccgcagg cggtgcaaaa
    96841 tcagggcgtg acggtgcgta aaaccggcga taccaacatt ctgaccattg ccttcgtctc
    96901 taccgatggc tcgatggata aacaggatat tgctgattat gttgccagta atattcagga
    96961 cccgttaagc cgcgtgaatg gcgtcgggga tatcgatgcc tatggttcgc aatattccat
    97021 gcgtatctgg ctggacccgg cgaaactcaa cagtttccag atgacggcta aagatgtcac
    97081 tgatgccatt gagtcacaga acgcgcagat tgcggttggg caacttggtg gtacaccttc
    97141 cgtcgataag caggcgctca acgccaccat taacgcccag tcactgctgc aaacaccaga
    97201 acagttccgc gatatcacct tgcgggtgaa tcaggacggc tcagaggtaa ggctgggcga
    97261 tgtcgccacc gtcgaaatgg gggcggagaa atacgattat cttagccgct tcaatggtaa
    97321 gccagcctcc gggttggggg taaaactggc ctccagcgca aacgaaatgg cgacagcgga
    97381 gctggtgctc aatcgtctcg acgagctggc gcagtatttc ccgcacggac tggaatacaa
    97441 ggtggcgtat gaaaccacct cgtttgttaa agcctccatt gaagacgtgg tgaaaacgct
    97501 gctggaagct atcgctctgg ttttcctcgt tatgtatctg ttcctgcaaa acttccgcgc
    97561 cacgctgata cccactatcg ccgtgccggt ggtgttgatg ggaaccttct ccgtacttta
    97621 cgccttcggt tacagcgtca acaccttaac catgttcgcg atggtgctgg cgatcggtct
    97681 gctggtggat gacgccatcg tggtggtgga aaacgtcgaa cgtattatga gtgaggaagg
    97741 actcactcct cgcgaagcca cacgtaaatc gatggggcag atccaggggg cactggtcgg
    97801 gattgcgatg gtgctttcgg cggtatttgt accaatggcc ttcttcggcg gcaccaccgg
    97861 tgccatctat cgccagttct ctattaccat tgttgcggcg atggtgctgt cagtactggt
    97921 agcgatgctc ctcactccgg ctctgtgtgc cacgctactt aagccactga aaaaaggtga
    97981 gcatcacggg caaaaaggtt tttttgcctg gtttaaccag atgtttaacc gcaacgccga
    98041 acgctacgaa aaaggggtgg cgaaaattct ccaccgtagc ctgcgctgga ttgtgattta
    98101 tgtcctgctg cttggcggca tggtgttcct gttcctgcgt ttgccgacgt cgttcttacc
    98161 gctggaagac cggggcatgt ttactacctc ggtacaattg cccagcggtt caacccaaca
    98221 acagaccctg aaagtcgttg agcaaatcga gaaatattac ttcacccatg aaaaagacaa
    98281 catcatgtcg gtgtttgcca ccgttggttc tggccctggg ggtaacgggc aaaacgtggc
    98341 gagaatgttt atccgcctga aagactggag cgaacgcgac agtaagaccg gcacctcgtt
    98401 tgccattatc gagcgtgcaa cgaaggcgtt taaccaaatt aaagaagctc gcgttatcgc
    98461 cagcagccca ccagcaatta gcggtcttgg tagttctgca ggttttgata tggagttgca
    98521 ggaccacgct ggagcgggtc acgatgcact gatggcagca cgtaatcagt tgctggcgct
    98581 ggcggcggaa aacccggagc taacccgtgt gcgccataac ggcctcgatg acagtccgca
    98641 gttgcagatt gatatcgacc agcgtaaagc tcaggcgctg ggcgttgata tcgacgatat
    98701 taacgacaca ctgcaaaccg cctggggttc gagctatgtg aatgacttta tggatcgcgg
    98761 tcgcgtgaag aaagtctatg tgcaggcagc tgcgccgtat cgcatgctgc cagatgacat
    98821 caatctctgg tatgtccgaa ataaagatgg cggcatggtg cccttctctg ctttcgcgac
    98881 ctcacgctgg gaaacaggct cgccgcgtct ggaacgctat aacggttatt ctgcggttga
    98941 gattgttggg gaagccgcac cgggggtcag taccggtacg gcgatggata ttatggaatc
    99001 gttagtgaag cagctgccaa acggctttgg tctggagtgg acggcgatgt cgtatcagga
    99061 gcggctttcc ggcgcgcagg ctccggcgct gtacgccatt tccttgctgg tggtattcct
    99121 gtgtctggcg gcattgtatg aaagctggtc ggtaccgttc tcggtaatgc tggtcgtgcc
    99181 gctgggggta atcggcgcgc tgctggcaac ctggatgcgc gggctggaaa acgacgttta
    99241 cttccaggtg ggcctgttaa cggtcattgg tttatcggcg aaaaacgcca tcctgatcgt
    99301 cgagtttgct aacgagatga accaaaaagg ccatgacctg tttgaagcga cgctccacgc
    99361 ctgccgtcag cgtttacgcc cgattctgat gacctcgctg gcatttatct tcggcgtatt
    99421 gccaatggca accagcacgg gtgccggttc cggtggtcag catgcggtgg gtactggcgt
    99481 aatgggcggg atgatttcag ctactattct ggctatttac ttcgtgccgc tgttctttgt
    99541 gctggtgcgc cgccgcttcc cgctgaagcc gcgcccggaa taagcaataa aaaaggcgac
    99601 atgccaatgt gtcgcctttt tcaactttcc gattaagaac ctgctcagcg ggttcttgct
    99661 gtttatactt tgtctcagga attacttacg aagcataact tcgataaagt ctttccagtt
    99721 ccccagttca cgttcaatca taacaacctc tcttataatt atgggtattc tacggaaaca
    99781 atataccgtg gtgaagctaa tttactcgat tgctgcgatg actacctccg ggggacaaac
    99841 cttatgtaaa tactatggtc ctgcggtgat aatttgtatg tgatacacag caacatttcg
    99901 agatattcat acggcatcta atactgattt aattctggtt aaaatacaga cagataacaa
    99961 gatgaatatt cttaatgttt acgttaaaaa tgtttaatat tatttaatag ttgttaattt
   100021 gaatacttcg ataatgttat attttctgat aatcatttgc aggcaaaatg ttttcaccct
   100081 taaatgagta tttattctca taaatcgaaa aaggattcat tatggttaca ctttacggca
   100141 tcaaaaattg tgacaccatt aaaaaggctc gccgttggct ggaagccaat aacatcgact
   100201 atcgttttca tgattaccgc gtcgatgggc tggacagcga attattgaac ggttttatca
   100261 acgaattagg ctgggaagcg ttactcaaca cccgtggtac aacctggcgt aaactggacg
   100321 aaactacccg caataaaatc accgatgcgg cctctgcggc ggcattaatg actgaaatgc
   100381 ctgcaattat caaacgtcca ttgctctgcg cgcctggtaa gcctatgctg ctgggtttca
   100441 gtgattccag ttatcagcaa tttttccatg aggtgtagtc tatgtcgtgc ccggttattg
   100501 agctgacaca acagcttatt cgccgccctt ccctgagtcc tgatgacgca ggatgtcagg
   100561 ctttgttgat tgaacgtttg caggcgatcg gttttaccgt tgaacgcatg gattttgccg
   100621 atacgcagaa tttttgggca tggcgtgggc aaggtgaaac gttagccttt gccgggcata
   100681 ccgacgtggt gccgcctggc gacgccgatc gttggatcaa tccgccgttt gaacccacca
   100741 ttcgtgacgg catgttattc gggcgcggtg cggcagatat gaaaggctcg ctggcggcga
   100801 tggtggtagc agcagaacgt tttgtcgcac aacatcccaa ccatacgggg cgactggcat
   100861 ttctgatcac ttctgatgaa gaagccagtg cccacaacgg tacggtaaaa gtcgtcgagg
   100921 cgttaatggc acgtaatgag cgtctcgatt actgcctggt cggcgaaccg tcgagtatcg
   100981 aagtggtagg tgatgtggtg aaaaatggtc gtcgcggatc gttaacctgc aacctcacca
   101041 ttcatggcgt tcaggggcat gttgcctacc cacatctggc tgacaatccg gtacatcgcg
   101101 cagcaccttt ccttaatgaa ttagtggcta ttgagtggga tcagggcaat gaattcttcc
   101161 cggcgaccag tatgcagatt gccaatattc aggcgggaac gggcagtaac aatgttattc
   101221 cgggtgaact gtttgtgcag tttaacttcc gcttcagcac cgaactgact gatgagatga
   101281 tcaaagcgca ggtacttgcc ctgcttgaaa aacatcaact gcgctatacg gtggattggt
   101341 ggctttccgg gcagccattt ttgaccgcgc gcggtaaact ggtggatgcg gtcgttaacg
   101401 cggttgagca ctataatgaa attaaaccgc agctactgac cacaggcgga acgtccgacg
   101461 ggcgctttat tgctcgcatg ggggcgcagg tggtggaact cgggccggtc aatgccacta
   101521 ttcataaaat taatgaatgt gtgaacgctg ccgacctgca gctacttgcc cgtatgtatc
   101581 aacgtatcat ggaacagctc gtcgcgtgat gagtggttct gcaagaggaa ataagcatgg
   101641 actggctggc taaatattgg tggattctgg tgattgtctt tttggtaggc gtcctgctga
   101701 acgtgattaa agatctcaag cgcgtcgacc ataagaaatt tctcgccaac aagccggagc
   101761 ttcccccgca tcgtgatttc aacgataagt gggacgatga cgacgactgg ccgaaaaagg
   101821 atcaaccgaa gaagtaagct gagtgctcaa ccgtaggccg gataaggcgt taacgccgca
   101881 tccggcaatg gtgaacgatc cctgatgcga cgctcacgcg tcttatcagg ccaggtaatc
   101941 tgtcttttac atcatttcaa tcacatcatc atcgccaggc ttaccgccac ttagcgcttg
   102001 gtcgaaataa tgtttcggaa tggtataacg caagtgatcc agggcaaatt gcatactgcg
   102061 attgtcgatc gcatggccta aatcctccac gatatccagc gtcacgtcac caccggcact
   102121 gattaacgct tcctgcgccg caactgcatg cgccagatcg ataaccggat cttcaccacc
   102181 atgaatcaag tgaattgtcg tggctgtaga cgcggtctcc ggcaggctcg catagcgtcc
   102241 gttaaaggca atgacgcgcg aagcaaggcc cggttcggct ttaatgctct ccagcgccat
   102301 aatcgcgcct tgtgagaaac caattagcgc cgtggcgtta gcccctactc cgctctgttt
   102361 ctgccagtag cgtaccgttt caataaacgt cggcatgatg gcatccacac gcgcctggcg
   102421 attatcttcc gtaataccct gcactgaaaa ccactgacgc cccgccggat taccgctcgg
   102481 ctccgcgcca ccgacactca ccaccagcgc atcaggaaac agcggtgcaa accagctgcc
   102541 tatttccccc attgccaccg ggttatcccc gacaccgtgg aaaagcagta acaactgttg
   102601 tgcaggttta tccgggcttt gaacaacaaa atggtcatgt ttcatggcga actccttaac
   102661 tgatgtcatt aattttacgc cgctgactca caatgaccat gggagaaaat tgaaggagtc
   102721 agtgaaaaaa ttgccattgc gttatgcgat cgcgcagacg ctcagtgcga acctcattta
   102781 gtgcgaacag cgcctgcgcg gcctcttccc gctggcgaac cagtaacatc ttgcggcctg
   102841 aaagtttaag tgtggtacat aactgcgcgt cactggcgtt tttctgtaaa cgcccacgca
   102901 gcgccggtaa tgccagttca ctggtttgta gcagacgcaa taagcaacct aacgatgtta
   102961 atagcggacg atgagcgaaa gcaaaaccgg ccagttcaag ccagtcgtcg tcagaaagga
   103021 tggcatcgtt tagtggatca acaggaagca tttcgccatt ccactgcgcg agagcttgcg
   103081 catcgcgacg taaacggtaa tgctcacgtt cagccagctg tttacccgca tcactcatcg
   103141 gtaacagcgc catcgccgta tagcaaccgc tgctggcttc ccgatgatta cccatccgca
   103201 ccagcacaaa accgcagcgt tgccagaaac gccataactc cccagtgtaa ccaaaactca
   103261 ccgaaagata gtcgaggtca tgcgtatatt gcaaagctcc agcaataagt tgtcgccctg
   103321 tgccttcccg ctgacgagcc ggatgaactg ctatccggct aacccgcctt ccgcgcaatg
   103381 tcgccgccag tggattgctg ccgtgcgccg ccagcgactg ggccaccaga ttaccccgcg
   103441 ggcgacgaaa acctgcccat accgcctgac tgagttcttg agataatccg ccctcatcca
   103501 ccagccacaa cgccccggca atctcgtttt cgccagccgc ctgtaaaaaa tgttgccctg
   103561 gtgcatccat catccggcgt aaatccagcg gcgaggtccg gtagtgcgca ccagacaata
   103621 actgataaac ctttaacggc gtttctggct cgcttcgcca taacgtttgt tcaaatgcgg
   103681 aaatgacgat attgccttgt ggtgtatggg tgaagttttc atcgttaaaa accagtgcct
   103741 cactaaccat tttttccagc gggcatccct gtgcccagcg gatcggctgt tgcagttcaa
   103801 aacggtgtaa atgcggaaag cgagcgcaaa atttcagcaa aaaaccacgt ccggtacctt
   103861 cgtagccctg caccgtagtg gttaacaacg ttcgaggaaa acgcgatacc agttgatgca
   103921 acaacggcgc aggtatggct gcggcttcgt cgaccaccag ccagtcggct tgctcatcgc
   103981 tggctaacaa ggcatccggt gcaataaagc gaaacttctc gcccgcaaat tgtgccagta
   104041 catccgttgc cgcttttgcg ggcgcggtga caatcgcact gcccgcaata cgagaaatga
   104101 gttgccctgc caacgccgac ttaccgcgcc cacgcgcagc cgttaccgct gccacgcccg
   104161 gcggcatggt cagtagctgc tgtaagagtt gctgttgttc tggttgtggt gcgccggtcg
   104221 cggggtgcca gtcagtacgg ggagtaaaat gcgccaacga gaacggctgg ttttgccgcc
   104281 agaggatagc gtcgttatcc gccgtaagta cgcgtttgag atgctggaca aaatgcggcg
   104341 tcgcaatagg gtcagggcaa tcactccagc gcagcgagtc ggcatcaggt tggttttccc
   104401 actcttccca tacagggagt aacaaaacca gccagcttcc cgctttcaac gttccgctaa
   104461 gtgctgcaaa ggcagcggca tcaaagccgt ggcgggcgtc gaataccgca tgccggaact
   104521 cgcgcccaag taaagtttgt agcgccgagg gtgaacagtg gttttcagca tctagctgcg
   104581 gcgaaatcca cagccagtcg ccaggtaagg catcacgcaa cttaagagca tgatcaaaac
   104641 accaaccctc ttccccgctc aacaccagca agcggcagat cccttcacgt ttcatttgcg
   104701 ctgttaatgt gtaaagcgca gtcagttcag ccatccctgc cccggaaatt aaatgctttt
   104761 accaaaagta ttgcattgtg ccggatcgcc gctgtcgaaa ccacgtttaa accagctgta
   104821 gcgttgctga gaagtgccat gtgtgaaact gtctggtact actcgcccct gactttgctg
   104881 ttgtaaacgg tcatcgccga tggcctgcgc cgcattcagc gcctcttcca gatcgccagt
   104941 ttccagaacg ccttgctgct gcatactatg cccccagaca ccggcaaaac agtcggcctg
   105001 gagttccata cgcacagata agcggtttac ttccgcctgc gtcgcgtttt gttgcagttg
   105061 acgaactttc ggctcgatgc ctaacagttt ctgcacatga tgaccgactt catgggcgat
   105121 aacgtacccc tgggcaaaat cgccatccgc gccaagtttg tctttcatgt catcatagaa
   105181 ggagagatcg atataaactg tgccatccgc cgggcaatag aacggcccca taatggactg
   105241 gcccgccccg cagccggtgc gcgtcattcc acggtacatg accagcttcg gttgctgata
   105301 ggtcttaccc atcttttcga actgttgtcc ccaggtgtct tccgtggttg ccagaatcac
   105361 cgaggtgaat tttgcggctt cgtcttcatt agggctaatt gaccgcgttg attgttgttg
   105421 agaaaccggc tgcccggtca tcaacccggt taaatcaacg ccatagtagc ctgcaaccag
   105481 cacgactatc agtaaaatca gcccgccttt accgctcggc agacggaaac cgggaccgcc
   105541 cattgacgga ccgccagagc tgttgcgcct gtcttcaaca ttgtcacttt cacgtcgccc
   105601 ttgccaacgc atagatacct caacaatata ttcattatag aaatgatcgt aggtggttca
   105661 gaggaagatt accacaggaa agcgggatga aatggcggag gaatgagcgt tcaatgcata
   105721 tattccgaga gcggttgtct tgccgtgcca gctgcacggc aagatgatga acagaaaaat
   105781 cagtccagct gtacacccag gcggcgggcg acggcttcat aggcttcgat caggccaccg
   105841 aggctctggc ggaaacggtc tttgtccatt ttctccagtg tttctttgtc ccacaggcgg
   105901 ctgccgtccg gggagaactc atcgcccagt accacttcac ctttgtacag accaaactcc
   105961 agcttgaagt cgaccagaat cagaccagca tcatcgaaca gttttttcag cacgtcgttc
   106021 gctttgtagg tcagctcttt catacgcgcc aggttctctt tgctcaccca gccaaaggtt
   106081 tcgcagtaag attcgttgac catcggatcg tgcatggcat cgtttttcag gaacagatcg
   106141 aacagcggcg ggttcagctc aataccttct tcgattccaa gacgtttcac cagagagcca
   106201 gcagcacggt tacgcacgac acactcaacc ggcaccatat ccagcttttt caccagacat
   106261 tcggtatcgg agagcagacg ctccatttga gtcgggatac ccgcttcagc cagtttgctc
   106321 ataatgaagt agttgaactt gttgttcacc atacctttgc gatcaaactg ctcaatgcgc
   106381 gcgccatccc ctgctgacgt atcattgcgg aattcgagca ccaacaggtc cgggttttcc
   106441 gtgctgtata cggttttcgc tttaccacga tacaactcag cttgcttttg catctttatc
   106501 actcctgggt gtgaattaac gttttaaaat cttttgctgt ctggtgtgcc ggatgttttg
   106561 tcggatgcgg cgtgaacgcc ttatccgaac tacgtccgac aaacaaattt cgtgcgaatt
   106621 accgctaaaa tcgcgttttc ctgccgacgc acacgtttgc gtatcatatc agaaaaaagg
   106681 gccggatgat tccagccctg tatttttact tgctaaacgc agcctggaag acagctacca
   106741 gcgcgtcgtt ctgactctga gtcagagtat gacctttcgg atcgatgaac tgtaggctgc
   106801 tgcggttatc taaatcgcca acctgcagtt tatagtcacc ggatgccagg cctggatcgc
   106861 tcgcgcccag ttcctgccag tcgctgtcag acagcggctt ataagttacg gccatgttgc
   106921 cctgcgaacg ggtgctgtcg gtcactttca tgcccacttt ttccagcgcc gctggcagac
   106981 gttgccaaac cacattgaac ggcccgcgta cgaccagcat tggtaaaccg gtgtcatcag
   107041 ctgcactttg tacgtccata gtggtggagg cacgattttg cgcagcgttc gcggcgtcag
   107101 tggcagattt atccagaccg gcggaaataa cgttcatcat ctccgtgctg taacgctgca
   107161 tggaagccgc gtctgcaacc ggtttgcccg cctgttccag gttcagcagt ttaaccgtaa
   107221 ccgcctgctg ataaccctgc ggcttaacag agatttgata acgaccacga tactgctcgt
   107281 cttcgtccag acggttccat tgtacccaat cggtggtcag tgtctgacca gcatcatcac
   107341 gttgggtgat ggtgtagttt ttcgcctgca gcacgctaac cacctgcggc cacagagtat
   107401 tgccacgacc attttccacc agcaatgaag cggtatcgcc cgtgaactgg gtacgcgcgc
   107461 cagaaaccag tgccaacggc tgggctggtg gacgaatgtc cagcgcctta ccgacagcac
   107521 cactaccgtt ggtcaccggg attgcataat caccggaggt caccggcaaa atcattccag
   107581 ccggggcatg aagctccgca agcggtgccg cttccaggta ggcttcatca ccactgacct
   107641 gacgcttata gcgtgagtca gaactacagg cagcgagtaa taaaacaagc gaaacacccg
   107701 caacctttgc caggcgcgac ttttgaacag agtaagccat caaatctccc taaactttac
   107761 agcaaaccgg catgcttaag cgccgctctg accgtctcac gaccactgtc ggtgattggt
   107821 gtcattggca ggcgcagcgt atcggtcgcc acaagaccca gttccttaca tgcccatttc
   107881 accgggattg gattgggttc gacaaatagt ttgttgtgta atggcatcag acgctgatta
   107941 ataacgcgtg cctcggcaaa atgcccttct gctgccagtt tgcacatctg ggccatatca
   108001 cgcgctgcga cgttagccgt aacggaaata actccatgac cgcccaattg catgaagtcc
   108061 agcgcgctcg catcatcgcc gctcagcaga acaaaatcat ctgaaaccag ctctttgatc
   108121 tggtttacac gcgttaagtt ccctgttgcc tctttgattc cgataatatt ttttactttc
   108181 gccagacggc ccaccgtttc cgggagcaga tcgcagccag tacgggacgg cacattatac
   108241 agaatttgcg gcaggtcagt atgctcagcg atggctttga aatgctgata caaaccttct
   108301 tgcgacggac gattgtagta aggggttacc gtcaggcagc cgacgatacc actgtcattg
   108361 aagcgctgcg tcaggctaat ggcttccgca gtagcgttag cgccggtccc ggcaattacc
   108421 ggaatgcgcc catcagccag atccagcgtc atcatcacca catcagcatg ttcgtcatga
   108481 tttaaggtag cggactcgcc agtggtgcca acagaaacga tcgccgaagt accgctggcg
   108541 acatgataat caatcagttt tttcaagcta gcccgacaga cattaccttt ttcatccatc
   108601 ggagtaacaa tcgcgacaat acttcccgtg aacatgggcc atcctctgtg caaacaagtg
   108661 tctcaatggt acgtttggta tggcattaaa agcaagcaga cagaaccgtt ctgattgttg
   108721 tatgcatgat ttttttatgc tttccttaag aacaactcac cccttaaagg aataaccagt
   108781 ttgacactgt catcgcaaca ttatctggtg atcactgcgt tgggtgccga tcgccctgga
   108841 attgtgaaca ccatcacccg tcatgtcagt agttgcggct gtaatattga agacagtcgc
   108901 ctggcgatgc tgggagaaga gttcacgttt atcatgctgc tttccggttc atggaatgcc
   108961 atcactctga ttgaatcaac gttaccgttg aaaggtgccg aactggatct tttaatcgtg
   109021 atgaagcgca cgacggcgcg tccgcgtccg ccaatgccag catctgtctg ggttcaggtc
   109081 gatgttgcag actccccgca tttaattgaa cgcttcacag cacttttcga cgcgcatcat
   109141 atgaacattg cggagctggt gtcgcgcaca caacctgctg aaaatgaacg ggctgcgcag
   109201 ttgcatattc agataaccgc ccacagcccc gcatctgcgg acgcagcaaa tattgagcaa
   109261 gcgttcaaag ccctatgtac agaactcaat gcacaaggca gtattaacgt cgtcaattat
   109321 tcccaacatg atgaacagga tggagttaag taatgaatcc actgaaagcc ggtgatatcg
   109381 caccgaaatt tagcttgccg gatcaagacg gagaacaagt taatttgacc gacttccagg
   109441 gacagcgtgt tctggtttat ttctacccga aagccatgac ccccggctgt accgtacagg
   109501 cctgcggctt acgcgataac atggatgagt tgaaaaaagc gggcgttgat gtgctgggta
   109561 tcagcaccga taaacccgaa aaactctccc gttttgcgga aaaagagctg cttaacttta
   109621 cgctcctgtc tgatgaggac caccaggtgt gcgaacaatt cggcgtctgg ggtgaaaagt
   109681 ccttcatggg caaaacctac gatggtattc atcgcatcag cttcctgatt gacgctgatg
   109741 gcaaaatcga acatgtcttt gacgatttca aaaccagcaa tcaccacgac gttgtgctga
   109801 actggctgaa agaacacgcc tgattacttt gctccattcc gtgctggctg cgcttgcggc
   109861 cagcatacct cacttctcgt gatcaagatc acattctcgc tttcccctgc gacacgggtg
   109921 tcgaatccat tttttgctga acgttaatga ccatcatttt tgtaccgttc agaatccagt
   109981 taatacataa cttattgaat agattgagtt aatcagaatg gcatccttta tacaatatga
   110041 aatgcaatgt ttcatatcat tttcaaggag ccgacatgaa ccgctttgtg gtggccgaac
   110101 cactgtggtg tacaggatgt aatacctgtc tcgctgcctg ttcggacgtg cataaaacgc
   110161 aaggtttaca gcaacacccg cgcctggccc tggcgaagac gtcaacaatc actgcccctg
   110221 tcgtgtgtca tcactgtgag gaagcccctt gcctgcaggt ctgcccggtc aatgccatct
   110281 ctcagaggga tgatgcgatc caactcaacg aaagcctctg tattggctgc aagctttgcg
   110341 ccgtggtctg cccatttggc gcaatcagcg cttcaggaag ccgtccggtg aatgcccatg
   110401 cgcaatatgt ttttcaggct gaaggctcac tcaaagacgg cgaagaaaac gtgccgcccc
   110461 aacatgcgtt gctgcgctgg gaaccgggtg tccagaccgt cgcggtgaaa tgcgacctgt
   110521 gtgatttctt gccagaaggt ccggcctgcg ttcgcgcttg cccgaatcag gcgttacggc
   110581 tgatcaccga cgatagcctg caacgtcaga tgaaagaaaa acagcgcctt gccgcaagct
   110641 ggtttgccaa tggcggggag tctccccttt ccctcactca ggagcaacac taatggatgc
   110701 cctgcaatta ttaacctggt cgctgatcct ctatctgttt gccagtctgg cttcgctgtt
   110761 tttactcggt ctggacagac tggctattaa gctttccggc atcacatcgc tggtgggcgg
   110821 cgtgattggc atcatcagcg gaattacgca attacatgct ggtgtaactt tagtcgccca
   110881 ttttgccacc ccttttgact ttgccgattt aaccctgcga atggatagcc tctcggcatt
   110941 tatggtgcta gttatctcct tgctggtggt ggtttgttcg ctctattcat tgacttatat
   111001 gcgcgaatac gagggcaaag gcgcggcggc gatgggcttc tttatgaatc ttttcatcgc
   111061 atcgatggtt gccctgctgg tgatggacaa cgctttttag ttcatcgtgc tgtttgaaat
   111121 gatgtcgctg tcttcctggt ttctggtcat tgccaggcag gataaaacgt cgatcaacgc
   111181 tggcatgctc tactttttta tcgcccacgc cggatcggtg ctgattatga tcgccttctt
   111241 gctgatgggg cgcgaaagcg gcagcctcga ttttgccagt ttcgcacgct ttcactttct
   111301 ccggggctgg cgtcggcggt gtttctgctg gcctttttcg gttttggcgc gaaagccggg
   111361 atgatgccgt tgcacagctg gttgccgcgc gctcaccctg ccgcaccatc gcacgcttcg
   111421 gcgttgatgt ctggcgtaat ggtcaaaatc ggtattttcg gcatcctgaa agtagcgatg
   111481 gatctgctgg cgcaaacggg tttgcctctg tggtggggca ttctggtgat ggcgatcggc
   111541 gcaatctccg cgctcctggg cgtgctatat gcgctggcgg aacaggatat caaacggctg
   111601 ctggcctgga gtaccgtcga aaacgtcggc attattttgc tggcagtcgg tgtggcgatg
   111661 gacggtctgt cactgcacga cccgctgctc accgtggttg gactgctcgg cgcactgttt
   111721 catctgctca accatgcgct gttcaaaggg ctgctatttc tcggcgcgga cgcgattatt
   111781 tcgcgtttgc atacccacga catggaaaaa atgggggcac tggcgaaacg gatgccgtgg
   111841 acagccgcag catgcctgat tggttgcctc gcgatatcag ccattcctcc gctgaatggt
   111901 tttatcagcg aatggtacac ctggcagtcg ctgttctcac taagtcgtgt ggaagccgta
   111961 gcgctacaac ttgcgggtcc tattgctatg gtaatgctgg cagtcactgg tgggctggca
   112021 gtaatgtgct tcgtaaaaat gtacggtatt actttctgtg gtgcgccgcg cagtacacac
   112081 gctgaagagg cacaggaagt gccaaatacg atgatcgtcg ccatgctact gctcgcggca
   112141 ctctgcgtat taattgcgct tagtgccagt tggctggcac cgaagataat gcatattgcc
   112201 catgcgttta ccaatacccc tcccgtcact gtcgccagcg gaatagcact tgtacccggc
   112261 acgtttcata cacgggtcac tccttcatta ctgttgctgt tactactggc gatgcctttg
   112321 ctgccaggcc tttactggct gtggtgtcgt tcgcgccgcg cagcgtttcg tcgcacagga
   112381 gatgcctggg catgcggcta cggctgggaa aacgcgatgg ctccgtcagg caatggcgtg
   112441 atgcagccgc tgcgtgtggt cttctctgcg ttatttcgtc tacgacaaca gctcgaccct
   112501 acgctgaggc taaataaagg tcttgcgcac gtcaccgcca gggctcagag cacagaaccc
   112561 ttctgggatg agcgggtgat ccgccccatc gtgagcgcca cccaacggct ggtcaaagaa
   112621 atacagcatc tgcaaagcgg cgactttcgt ctctattgcc tgtatgtggt cgccgcactg
   112681 gttgtgctgc taatcgctat tgccgtctaa ggaaatcacc atgagacaaa ctctttgcga
   112741 cggatatctg gtcatttttg cgttagcaca ggccgtgatt ctgctgatgc taaccccact
   112801 ttttacgggt atttcccggc agatacgcgc gcgtatgcac tcccgccgcg ggccggggat
   112861 ctggcaggat tatcgcgata tccacaaact gtttaaacgc caggaagttg cgccgacatc
   112921 ttcaggtctg atgttccgcc tgatgccgtg ggtattaatc agcagcatgc tggtgctggc
   112981 gatggcctta ccactattta ttaccgtttc cccttttgcg ggcggcggcg atctgatcac
   113041 ccttatctat cttcttgccc tgtttcgttt tttctttgct ctttccgggc tggataccgg
   113101 aagtccgttt gcgggagtcg gtgccagtcg cgagttgacg ctcggcattc tggtcgaacc
   113161 aatgcttatt ctctcactgc tggtattggc gctgatagca ggttccacgc atatcgagat
   113221 gatcagcaat acgctggcta tgggctggaa ctcgccgcta accaccgtac tggcgttact
   113281 ggcctgtggt tttgcctgct tcattgagat gggaaaaatt ccctttgatg ttgctgaagc
   113341 agaacaggaa ttacaggaag gcccgctgac cgaatattcc ggtgccgggc tggcgctagc
   113401 gaaatggggg ctggggctga aacaggtcgt gatggcagca ctgtttgtgg ccctgtttct
   113461 gccctttggg cgcgcgcaag aactttctct cgcctgcctg ctgacttcac ttgtcgttac
   113521 gctgctcaag gttttgctga tttttgtact ggcctcaatc gcagaaaaca cgctggcacg
   113581 cgggcgtttt ttactcattc accatgtgac ctggcttggc ttcagccttg ctgcgcttgc
   113641 atgggtcttt tggttaaccg gtctgtaagg agcactgacg gaatatggaa aatcttgctc
   113701 tgacgacgtt aatgctgcct tttatcggcg cactggtcgt ttcgttttcg ccacaacgtc
   113761 gggccgccga atggggggtt ttgttcgccg cgctgaccac gctgtgcatg ttgtcgctga
   113821 tctccgcgtt ttatcaggcc gataaagttg ccgtcacgtt gacgttggtc aacgtggggg
   113881 atgtggcatt gtttggcctg gtcattgatc gcgtgagtac gctgattctg tttgtggtgg
   113941 tgtttctcgg tttgctggtc acgatctact ccacgggtta tctgacggat aaaaatcgcg
   114001 aacacccgca taacggcacg aatcgttatt acgcattttt gctggtgttt atcggcgcga
   114061 tggcgggact ggtagtctcc tcgacgctgc tcggtcagtt gttgtttttt gaaattacgg
   114121 gcggctgctc ctgggcgttg atcagttatt accagagcga taaagcgcag cgttcagcac
   114181 taaaagcgtt gcttatcacc catatcggtt cgttagggtt gtatcttgcc gccgccacgc
   114241 tgtttttgca gaccggaacg tttgtgctta gcgcgatgag cgagttacat ggcgacgcac
   114301 gttatctggt ttatggcggc atcctgtttg ccgcgtgggg gaaatcggcc cagctaccga
   114361 tgcaagcgtg gctaccggac gcaatggaag cgccaacacc gatcagcgcc tatctccacg
   114421 ccgcatcgat ggtgaaagtg ggcgtttaca tttttgcccg cgccattatc gacggcggca
   114481 atatcccgca tgtgattggc ggcgttggca tggtcatgac actggtcacc attctttatg
   114541 gctttctgat gtatttgcca cagcaggata tgaagcggtt gctggcctgg tcgaccatca
   114601 ctcaacttgg ctggatgttc ttcggcttgt cgctctccat cttcggctcg cggctggcgc
   114661 tggagggtag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg tttttccttg
   114721 tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg cgtggcgtat
   114781 tgcacaccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggca attaccggtg
   114841 tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt tttgcgttgt
   114901 cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa tcggtcgcca
   114961 gtttcgcctg gtttattcgc tggtttggtc gcgtcgtgcc gggcaaaccg agcgaggccg
   115021 tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta ctgattgtga
   115081 tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat gatgaatgac
   115141 cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc tgtttgtgat
   115201 tagcgtcaaa agctatcgcc tgtcatgcgg attttacgcc tgccagtcgc tggtgctggt
   115261 gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga tctggtccgc
   115321 cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg ctgcacgaaa
   115381 tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg cactgctcgc
   115441 ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac cgatggctac
   115501 cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc tgctgtgcat
   115561 tgtcagccaa cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg aaaacggctc
   115621 ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa taggtatcgc
   115681 taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa tatggcgtac
   115741 ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag atgagttatt
   115801 ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc tgttttgcct
   115861 gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc ttagggatca
   115921 cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca ggagaaatat
   115981 tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc gccattcttg
   116041 gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat gaagtggcac
   116101 acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat ctgtttttgt
   116161 tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg gcgatcgaag
   116221 ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca tcgctggaat
   116281 cagcatggaa gtacatcatt atttgtactg ttggtgtcgc ttttggtctg ttcggtaccg
   116341 tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg atatcctgga
   116401 gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg gcctttgtgt
   116461 ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc tggctgccgg
   116521 atgctcacag tgaagctccg agtccggtca gcgccctgct ctccgccgta ttgctgaact
   116581 gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaggccatc ggcagcgatt
   116641 tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg gcatttttca
   116701 ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag aacatggggc
   116761 tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg ctgctgcaca
   116821 ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat gtactgctca
   116881 agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg ccatttaccg
   116941 ccgtgctgtt tggcggcggt gcgctggcgc tggcgctggc agggatgccg cccttcaaca
   117001 tttttcttag cgaatttatg accgttaccg caggactggc acgtaatcac ctgctgatta
   117061 tcgtcctgct gttattgctg ttaacgctgg tgctggcggg cctggtacgg atggctgcgc
   117121 gggtgttaat ggcgaaaccg ccgcaggccg ttaaccgggg tgatctcggc tggttgacca
   117181 cctcgccaat ggtgattctg ctggtcatga tgctggcgat gggaacgcat attccacaac
   117241 ctgtcatcag gatcctggcg ggcgcttcca ctatagtcct ctcagggacg cacgacctgc
   117301 ctgcacaacg tagcacctgg catgattttt tgccttcagg caccgcatct gtttcggaga
   117361 aacacagtga acgttaattc atcgtcaaat cgtggcgaag cgattctcgc cgccctgaaa
   117421 acgcagttcc ccggcgcggt gctggatgaa gagcgacaaa cgcctgaaca ggtcaccatt
   117481 acggtgaaaa tcaatctgct gcctgacgtt gtacattatc tttattatca acatgatggc
   117541 tggcttccag tcctgtttgg caacgacgag cggacactta acggtcatta cgcggtttat
   117601 tatgcccttt caatggaagg ggccgaaaaa tgctggatcg tggtgaaggc actggtcgat
   117661 gccgacagtc gggagtttcc gtcagtcaca ccgcgcgtcc ctgccgcggt ctggggcgag
   117721 cgagaaattc gcgatatgta cgggctgatt ccggttggcc tgccggatca gcgtcgcctg
   117781 gtgttgcccg atgactggcc ggaagatatg catccgctgc gcaaagatgc gatggattat
   117841 cgactgcgcc ctgaaccgac gacagattcc gaaacgtatg cgtttatcaa tgagggcaac
   117901 agcgatgcgc gggtgatccc tgtcggcccg ctgcatatca cctcctatga accgggtcac
   117961 ttccgcttgt ttgtggatgg cgagcaaatt gtcgatgctg attaccgcct gttttatgtc
   118021 catcgcggca tggagaaact ggcagaaacg cggatgggct acaacgaagt gaccttctta
   118081 tcggaccgcg tgtgtgggat ttgcggtttt gcccacagtg tggcctatac caactcggtt
   118141 gaaaatgcac tggggattga ggtgccgcaa cgagcacata ctattcgctc gattctgctg
   118201 gaagtcgagc ggctacacag tcatttgctc aaccttggcc tctcctgcca ttttgttggt
   118261 tttgataccg gctttatgca atttttccgc gtgcgggaaa agtcgatgac gatggcggaa
   118321 ttgctgaccg ggtcgagtaa aacctacggt ctgaatctga ttggtggtgt tcgccgcgat
   118381 attctcaaag agcaacgtct gcaaacgctg aaactggtgc gcgagatgcg cgccgacgtg
   118441 tcggagctgg tagagatgct gcttgctacg ccgaatatgg aacaacgcac tcagggcatt
   118501 ggcattctcg accgacaaat cgcccgtgat tatagccctg tagggccgct gatccgcgca
   118561 gtggttttgc ccgtgatttg cgctttgatc acccctacgc cgactacggc aatattccaa
   118621 aaacactgtt tacctttacc ggcggcgatg ttttctcccg cgtgatggtc cgtgtcaaag
   118681 agacgtttga ttcgctggca atgctggaat ttgccctcga caacatgccg gataccccac
   118741 tgctgaccga aggctttagc tataaacctc acgcattcgc gctgggcttt gttgaagcgc
   118801 cacgcggtga agacgtgcac tggagcatgc tcggtgataa ccaaaaattg ttccgctggc
   118861 gctgtcgtgc cgccacctac gccaactggc cggtgttgcg ttacatgctg cgcggcaata
   118921 ccgtttctga cgcaccgctg attatcggta gccttgatcc ctgctactcc tgtaccgacc
   118981 gtgtgacgct ggtagatgtg cgcaagcgcc agtcaaaaac cgtgctgtat aaagagatcg
   119041 aacgctacgg cattgatcgt aaccgttcgc cgctgaagta aggacagaag atgctgaagt
   119101 tactgaaaac tattatgcgc gccggaaccg cgacggtgaa atatcccttc gcgccactgg
   119161 aggtcagccc aggctttcgc gggaaaccgg acctgatgcc cagccaatgt attgcctgcg
   119221 gtgcctgcgc ctgtgcttgt ccggcaaatg cgctgactat ccagaccgac gaccagcaaa
   119281 attcgcgcac ctggcagctc tatctggggc gttgtattta ctgcggacgt tgtgaagaag
   119341 tgtgcccgac cagagccatc cagcttacca ataactttga actgaccgtc acccataaag
   119401 ccgatctcta tacccacgcg acgttccatc tacaacgttg cagccgttgc gaacgcccgt
   119461 ttgccccgca aaaaaccgtc gcactggctg ctgaattgtt ggcacagcaa caaaatgctc
   119521 cacaaaaccg cgaaatgtta tgggcgcaag cgagcgtctg cccggagtgc aaacaacgcg
   119581 cgacgctgat caacgacgat acagatgtac cgctggtggc taaggagcag ctatgagtcc
   119641 agtgcttacg caatatgtca gccagcccat cacgctggac gagcaaacgc aaaagatgaa
   119701 gcggcatttg ctacaggata tccgtcgctc ggcttacgtt tatcgcatcg attgcggcgg
   119761 ctgcaacgcc tgtgaaatcg aaatttttgc tgccatcacc cccgtattcg acgcagaacg
   119821 ttttggcatt aaggttgttt catcaccgcg tcacgccgat attttgttat ttactggcac
   119881 agtcacccgg gcgatgcgta tgcctgcact tcgggcgtat gagtctgccc ccgatcataa
   119941 aatctgtgtt tcctacggcg cgtgcggtgt cggcggcggt attttccacg atctctacag
   120001 cgtctggggc ggcagcgaca ccattgtccc cattgatgtt tggatccccg gctgcccgcc
   120061 aacaccggcc gccaccattc acggtttcgc cgtggcgctc ggtttgctgc aacagaagat
   120121 tcacgcggtg gattatcgcg atcccaccgg ggtgactatg caaccgttgt ggccgcagat
   120181 cccgccatca cagcgtatcg ccattgagcg agaagcgcgg cggctggcgg gctatcgtca
   120241 ggggcgagaa atttgcgatc ggctcctgcg ccatttaagc gacgatccta caggaaatcg
   120301 ggttaacacc tggttgcgcg atgccgacga tctacgtctc aatagtatcg ttcagcaact
   120361 ctttcgcgta ctccgggggt tacatgactg aagagtgcgg ggaaattgtt ttctggacgc
   120421 tgcgaaaaaa gtttgtcgcc agtagcgacg agatgccgga acacagctct caggtaatgt
   120481 attactcgct ggctatcggt catcacgttg gcgtgattga ttgtctgaat gtcgccttcc
   120541 gctgcccact gacggaatac gaagattggc ttgcactggt cgaagaggag caagcccgac
   120601 gtaagatgct gggggtgatg acttttggtg agattgttat tgacgccagc cacaccgccc
   120661 tgttgacccg ggcattcgcg ccactggcag atgacgcgac gtctgtgtgg caagcgcgta
   120721 gcattcaatt cattcatctg ttggatgaaa ttgtgctgga accggctatc tatctgatgg
   120781 ccagaaaaat tgcgtgagaa ggatttctca ttaataagga ctgttgatgg ctatgtcaga
   120841 cgaagcgatg tttgccccgc cgcaaggaat aacaattgaa gcggtaaacg gaatgctcgc
   120901 ggagcggtta gcgcagaaac acggcaaggc gtctttatta cgcgccttca tcccgctgcc
   120961 gccgccgttc agcccggtac aacttattga actgcatgtt ctcaaaagca acttctatta
   121021 ccgctaccat gatgatggca gcgatgtgac ggcaacaaca gagtatcagg gcgagatggt
   121081 cgattattcg cgttacgccg tccttctcgg cagtagtgga atggcggagc tacgctttat
   121141 tcgtacccac ggcagccgtt ttactcccca ggattgcaca ctgtttaact ggcaggcacg
   121201 cattatcacc ccggttctgc aatcatggct caatgatgaa gcacagcagg tggcgctgcg
   121261 tttgctggag aaagatcgcg atcatcatcg ggtactggtg gatatcacta atgcagtgct
   121321 gtcacatctt gatctcgacg atctgatcgc tgacgtcgct cgtgagatcc atcatttttt
   121381 cggtctggct tcagtcagta tggtactggg cgatcatcga aagaacgaga agttcagcct
   121441 gtggtgcagc gatctttctg cctcacattg tgcgtgtctg ccacacaata tgcctggcga
   121501 cagtgtattg ctgacacaaa cgctacaaac ccgacaaccg accttgacgc accgtgcaga
   121561 tgatctgttt ctctggcaac gcgacccgtt attactctta cttgcatcta acggctgcga
   121621 atctgcgctc cttataccgc ttacctttgg caaccataca ccgggtgcat tgttgctggc
   121681 gcatacctct tccactctct ttagtgagga aaactgccag ctactacaac acatagccga
   121741 tcgcatcgct attgccgttg gcaatgccga tgcctgccgt agaatgaccg atttgcagga
   121801 aagtttgcag caagaaaacc accagcttag caagcagctc ctttcgaatc tgggcgtcgg
   121861 tgacattatc tatcaaagcc aggcaatgga agacctgctc cagcaggtag atattgtggc
   121921 gaagagcgac agtacggtgt tgatttgcgg tgaaaccgga accggcaaag aggtgatcgc
   121981 cagagcgatc catcaactta gcccgcgaca cgacaagccg ctggtcaaaa tcaactgcgc
   122041 tgccatcccc gccagtcttc tggaaagtga gttattcggt catgacaaag gggcgtttac
   122101 tggtgcgatt aatacccatc gtggtcgttt tgaaattgcc gatggcggca cgttgtttct
   122161 cgatgaaatt ggcgatctgc cgttagaact tcagcctaaa ctgctgcgcg tattgcagga
   122221 acgggagatt gagcgtctcg gcgggagtag aacgatcccg gtaaatgtca gagtcattgc
   122281 cgccaccaac cgtgatttgt ggcaaatggt tgaagatcgc cagtttcgca gcgatctctt
   122341 ttatcgcctg aatgtcttcc cactggaatt gcctccgctg cgcgaccgtc cggaagatat
   122401 ccctctttta gcaaagcatt tcacgcaaaa aatggcgcgc catatgaatc gttcaattga
   122461 cgccatcccg accgaggcgc tacgccagtt gatgtcgtgg gactggccgg gcaacgtgcg
   122521 cgagctggaa aacgtgattg agcgagcggt actgctgact cgcggtaaca gtctgaattt
   122581 acatcttaat gtccgacaaa gccgtttact gccgacgcta aatgaagatt cagcgcttcg
   122641 cagttcaatg gcgcagttgc tgcacccgac gacgccagag aatgacgaag aagaacgtca
   122701 gcgcattgtt caggtattgc gagaaaccaa tggcattgtt gccgggcccc gtggcgcggc
   122761 gacacgatta gggatgaagc gcaccacgct gctgtcacga atgcagcgtc tgggaatctc
   122821 ggttcgcgag gtgttgtaat ctgcttttgc aggagtatgc atgagaaaca aactctcttt
   122881 cgacttgcag ttgagcgcca gaaaagcggc aatcgctgaa cggattgccg cccataaaat
   122941 tgcccgcagt aaagtgtcgg tctttttaat ggcgatgtcc gctggcgtgt ttatggcgat
   123001 cggatttact ttttaccttt ccgttatcgc cgatgccccg tcttcatagg cattaaccca
   123061 tctggtgggc ggcctttgct ttacacttgg ctttattttg ctggcggttt gcggcaccag
   123121 cctgttcacc tcgtcggtaa tgacggtaat ggcaaaaagt cggggcgtta ttagttggcg
   123181 aacttggctg attaacgcac ttctggtggc ctgcggtaat ctggcaggta ttgcctgttt
   123241 cagtttgtta atctggtttt ccgggctggt gatgagtgaa aacgcgatgt ggggagtcgc
   123301 gattttacac tgcgccgagg gcaaaatgca tcatacattt actgaatctg tcagcctcgg
   123361 cattatgtgc aatctgatgg tttgcctggc gctgtggatg agttattgcg gacgttcgtt
   123421 atgcgacaaa atcgtcgcca tgattttgtc catcaccctg tttgtcgcca gtggctttga
   123481 gcactgtatc gccaatttgt ttgtgattcc gttcgccatt gccattcgcc atttcgcccc
   123541 tacctccttc tggcaactgg cgcacagtag cgcagacaat tttccggcac tgacggtcag
   123601 ccattttatt accgccaatc tgctcccggt gatgctgggt aatattatcg gcggtgcagt
   123661 gctggtgagt atttgttatc gggctattta tttacgtcag gaatcctgaa aaatcagccc
   123721 ggcgaaacag ttcgtcgggc tgaagattat tcttgcgcga tttgcccatc gggccaggcg
   123781 tggatcacgg ctttgatcag cgtcgccaat ggaatggcga agaatacgcc ccagaatccc
   123841 cacaaaccac cgaagatcac caccgataaa ataatcacca gcggatgcag gttaaccgct
   123901 tcggagaaca acaccggtac tagcaggttg ccgtccagcg cctgaataat cagatacacc
   123961 gcgaagcagc tccagaattc agtacctgcg ccaaactgga atagcgccac gccaaccacc
   124021 ggaatggtca ccacaaatgc gccaatgtac ggaataagaa ccgagaagcc gaccagcact
   124081 gccagcagca gcgaatagtt cagcccgaag agcaagaacc ccagccaggt ggcgatcccc
   124141 accacgatca tctccagcac tttgccgcgg atatagttgg tgatttgttg attcatctcc
   124201 ttccacacct gtcctgccag cccacggtta cgcggcagca cccggcgaac ggcgttcagc
   124261 atctgctctt tgtctttcag caggaagaag accatcaatg gcaccagcac cagatagacg
   124321 gctatggtca gcaaaccgac cagcgaggcg agggaaattt tcaccaccga atcgcccatg
   124381 gtcagcatcc gactgcgcat attttcggcc attgcatcaa taatgcccgc gtccattaac
   124441 accggatagc ggcgcggcaa cgtggcggca aagtcagaaa gcttattgag catccccggc
   124501 atatcgcgga ttaagtagat gccctgttgc caggcgatag gcagtaccac gaacgccatc
   124561 agtagcaata taccgacaaa aaccaccaat acaatcgacg tcgcccagcg gcgggagcag
   124621 ccaatagatt gcaggcgcac ggttggccat tccagcaaat aagccagcac aatagccacc
   124681 agcaacggag caagcaggcc actaaagaaa aagataatgc caaatccggc aactaaaata
   124741 accagcaagg caatcgcttc cgggtcgcta aaacggcggc gataccattg catcaacatt
   124801 tcgagcataa agaccttccc tgaacctcaa gagcgggatt gcgatccgca attgtatcga
   124861 aatgtcacaa aaaagacttc gctttttatg acggattcag gaaactgaaa agtcatttga
   124921 gtgggctaat cttcgccgtt acactcaaag gcggcgaggt gggaacgata tttcacagta
   124981 tcggtcaaat gactatcttc agaaatacag gatagaggtt atgttcaggc agttgaaaaa
   125041 aaacctggtt gcaaccctca ttgctgctat gaccattggt caggtagccc cggcgtttgc
   125101 cgacagcgca gacaccttgc cggatatggg aacctccgca ggaagcacgc tttccattgg
   125161 tcaggaaatg cagatgggcg actattatgt ccgccagcta cgcggcagcg cgccgttaat
   125221 taatgacccg ctgttaacgc aatatattaa ttcgctgggg atgcgtctgg tttcgcatgc
   125281 caattcggtt aagacaccgt ttcatttttt tctgatcaac aacgacgaaa ttaacgcctt
   125341 tgctttcttt ggcggcaacg tggtgctgca ctctgccctg ttccgttatt ccgataacga
   125401 aagtcaactg gcttcagtta tggcgcacga aatctcccac gtcacccaac gtcacctggc
   125461 gcgagcgatg gaagatcaac aacgcagcgc gccgctgacc tgggtcggcg cgttaggttc
   125521 tattttactg gcgatggcca gtccgcaggc ggggatggcg gcgctgaccg gtacactggc
   125581 gggaacgcgc caggggatga tcagtttcac ccagcaaaat gaacaggaag cggaccgcat
   125641 tggtattcag gtgctgcaac gctcgggatt cgatccacag gcgatgccga ccttcctgga
   125701 aaaattgctc gatcaggcgc gttactcctc gcgcccgccg gaaattttat tgactcaccc
   125761 gttgccggaa agtcgtctgg cagatgcccg caaccgtgct aatcagatgc gcccgatggt
   125821 ggtgcagtcg tcggaagatt tctatctggc gaaagcgcgc acactgggga tgtataattc
   125881 cggacgtaac cagctcacca gtgatttgct ggatgaatgg gcgaaaggaa acgttcgtca
   125941 gcaacgagcg gcgcaatatg gtcgtgcttt acaggcgatg gaagccaata aatacgacga
   126001 ggcgcgtaaa acgctgcaac cgttactggc ggcagaacct ggcaacgcat ggtatctcga
   126061 tctggctacc gatatcgatc ttgggcaaaa caaagccaat gaggcaatca atcgcctgaa
   126121 aaatgcccgc gatttacgca ccaatccggt gttgcagctc aacctggcga acgcttatct
   126181 gcaaggcggt caaccacaag aagcggccaa tattctgaat cgctacacct ttaataataa
   126241 agatgacagc aacggctggg atttgctggc acaggcggaa gccgcgctaa ataaccgcga
   126301 tcaggagctg gctgcgcgag cagaaggtta tgcgctcgcc ggacgactcg atcaggccat
   126361 ttcgctgttg agtagcgcca gttcgcaggt gaaattaggc agcctgcaac aagcgcgtta
   126421 cgatgcgcgc atcgaccagt tgcgccagct gcaggaacgc tttaagcctt ataccaagat
   126481 gtaattcgca ttaaaggaag aacaatgacc aaacaggtaa aaatctacca taacccacgc
   126541 tgttcaaaga gccgggaaac gctgaacctg ctgaaagaaa acggcgtgga gccagaagtg
   126601 gtgctctacc ttgagacacc cgccgatgcg gcaacgctgc gcgatttgct gaaaatgctg
   126661 gggatgaata gcgcccgtga attgatgcgc cagaaagagg atctttataa agaactaaac
   126721 ctggctgaca gttcgcttag cgaagaggcg ctgattcagg caatggtgga taatccgaag
   126781 ctgatggagc gcccgattat ggtcgcgaat ggcaaagcgc ggattggtcg tccaccggag
   126841 caggtactgg aaatcgtggg ttaagatgtg ctccatcgct agttgaagca cattgccgga
   126901 tgcgacgctt gccacgtctt atccggccta cgaatactat ggattaactt cgcgtaggtc
   126961 aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa tttctttcac
   127021 aaacggaatg gtcagcttac gttgcgcggt aatcgacgca cgatccaact gatccaacgt
   127081 cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc
   127141 cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact tatcttcatc
   127201 agaaagtggc tgcaatttgt agatctgccc ccagtcgaga cgcgacgcga gatccggtaa
   127261 tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg ttttgcccga
   127321 ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat cgcctgcaat
   127381 acactcaatg ttgtcgatac agaccagcga caaatgctcc ataccgtcga gcacttccgg
   127441 aacaaaccag gtgcgtttat ccagcgggac atagcccacc gcatcgccac gctgcgacaa
   127501 ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg cccagagata
   127561 gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta aagaggagtt
   127621 atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg gcaaagagag
   127681 ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg agaagtttac
   127741 cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta cattatttgt
   127801 ctgttatatc cgcgtcttct gcgtccagca ccacttcttc agggcgcagc acactgatca
   127861 gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata ccttttaact
   127921 ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc acggaagtca
   127981 ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca cgaataccgg
   128041 aagcaccaat gacaccataa agcagcagcg aaacgccgcc catcaccggc aatgggatca
   128101 tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa atcgccgccc
   128161 cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg ttttctccgt
   128221 aagtggtatt tggcgtagag ccaaagaagc cggagatcac ggtcgacagg ccgttagcaa
   128281 acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata ttagccgtta
   128341 ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga atagtcagaa
   128401 tggcaaacca ctcgaagcgc ggcgtataga gggttggcag tgcaaaccag tgagcattaa
   128461 taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtacccc accagcacgc
   128521 caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct aaaaccgtga
   128581 ccgccagggt ggtaatagag atgatgatgg ttttggagtt tggcgtttgc ccttcagccg
   128641 ggagtaaacc cgccatgccg gcagctacgc ccgccagctc cagaccgatg acggcaacga
   128701 ttgcgcccat tgccgcaggt ggaaacagca cgtccaacca gccggtcccc gctttcttca
   128761 cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc agcgcgactt
   128821 cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg gaaccaagat
   128881 aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata ccgttaaata
   128941 acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt gcaccaaaca
   129001 tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt ctttcactca
   129061 ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata ggcgctttac
   129121 tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta tttcgtacca
   129181 aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc
   129241 tggtcaatcg atgcagtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata
   129301 ccttctggcg cagcgaccag cactagaact ttgatgctgc tgcagcccgc ttttttcagc
   129361 aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc
   129421 atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct
   129481 tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacgttttc cagcacaccg
   129541 tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt acctttgatc
   129601 tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg
   129661 gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg
   129721 gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact
   129781 tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaaat cgacggatta
   129841 tacctccttt cttcaaggcg gcaatattat tttcgttgac tttagtcaaa atgataacag
   129901 tttgagataa agttatttta tattcaaatg gttatgaaag aagattattc catccgaaaa
   129961 ctaacctttg ccctggcaca agtcttcttt cgccgcgcgc ccggggaaaa gacgtgcaaa
   130021 aaggttgtgt aaaacagtct cgcaaacgtt tgctttccct gttagaattg cgccgaattt
   130081 tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt gaccgataaa acctctctta
   130141 gctacaaaga tgccggtgtt gatattgacg cgggtaatgc tctggttgga agaatcaaag
   130201 gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg tctgggcggc ttcggtgcgc
   130261 tgtgtgcatt gccgcaaaaa tatcgtgaac ccgtgctggt ttccggcact gacggcgtag
   130321 gtaccaagct gcgtctggca atggacttaa aacgtcacga caccattggt attgatctgg
   130381 tcgccatgtg cgttaatgac ctggtggtgc aaggtgcgga accgctgttt ttcctcgact
   130441 attacgcaac cggaaaactg gatgttgata ccgcttcagc ggtgatcagc ggcatcgcgg
   130501 aaggttgtct gcaatcgggc tgttctctgg tgggtggcga aacggcagaa atgccgggga
   130561 tgtatcacgg tgaagattac gatgtcgcgg gtttctgcgt gggcgtggta gaaaaatcag
   130621 aaatcatcga cggctctaaa gtcagcgacg gcgatgtgct gattgcactc ggttccagcg
   130681 gtccgcactc gaacggctat tcgctggtgc gcaaaattct tgaagtcagc ggttgtgatc
   130741 cgcaaaccac cgaacttgat ggtaagccat tagccgatca tctgctggca ccgacccgca
   130801 tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga tgtgcatgcc attgcgcacc
   130861 tgaccggcgg cggcttctgg gaaaacattc cgcgcgtatt gccagataat acccaggcag
   130921 tgattgatga atcttcctgg cagtggccgg aagtgttcaa ctggctgcaa acggcaggta
   130981 acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg cgtcgggatg attatcgccc
   131041 tgcctgctcc ggaagtggac aaagccctcg ccctgctaaa tgccaacggt gaaaacgcgt
   131101 ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg cgtggttatc gaataatgaa
   131161 tattgtggtg cttatttccg gcaacggaag taatttacag gcaattattg acgcctgtaa
   131221 aaccaacaaa attaaaggca ccgtacgggc agttttcagc aataaggccg acgcgttcgg
   131281 ccttgaacgc gcccgccagg cgggtattgc aacgcatacg ctcatcgcca gcgcgtttga
   131341 cagtcgtgaa gcctctgacc gggagttgat tcatgaaatc gacatgtacg cacccgatgt
   131401 ggtcgtgctg gctggtttta tgcgcattct cagcccggcg tttgtctccc actatgccgg
   131461 gcgtttgctg aacattcacc cttctctgct gccgaaatat cccggattac acacccatcg
   131521 tcaggcgctg gaaaatggcg atgaagagca cggtacatcg gtgcatttcg tcaccgatga
   131581 actggacggt ggcccggtta ttttacaggc gaaagtcccg gtatttgctg gtgatacgga
   131641 agatgacatc accgcccgcg tgcaaaccca ggaacacgct atttatccac tggtgattag
   131701 ctggtttgcc gatggtcgtc tgaaaatgca cgaaaacgcc gcgtggctgg atggtcaacg
   131761 tctgccgccg cagggctacg ctgccgacga gtaatgcccc cgtagttaaa gcgccagctc
   131821 tgccgctggc gtttttcaat tcacctgtaa atcgcaagct ccagcagttt ttttccccct
   131881 tttctggcat agttggacat ctgccaatat tgctcgccat aatatccagg cagtgtcccg
   131941 tgaataaaac ggagtaaaag tggtaatggg tcaggaaaag ctatacatcg aaaaagagct
   132001 cagttggtta tcgttcaatg aacgcgtgct tcaggaagcg gcggacaaat ctaacccgct
   132061 gattgaaagg atgcgtttcc tggggatcta ttccaataac cttgatgagt tctataaagt
   132121 ccgcttcgct gaactgaagc gacgcatcat tattagcgaa gaacaaggct ccaacgctca
   132181 ttcccgccat ttactgggca aaattcagtc ccgggtgctg aaagccgatc aggaattcga
   132241 cggcctctac aacgagctat tgctggagat ggcgcgcaac cagatcttcc tgattaatga
   132301 acgccagctc tccgtcaatc aacaaaactg gctgcgtcat tattttaagc agtatctgcg
   132361 tcagcacatt acgccgattt taatcaatcc tgacactgac ttagtgcagt tcctgaaaga
   132421 tgattacacc tatctggcgg tggaaattat ccgtagcgat agcatccgtt acgcgctgct
   132481 ggagatccca tcagataaag tgccgcgctt tgtgaattta ccgccagaag cgccgcgtcg
   132541 acgcaagccg atgattcttc tggataacat tctgcgttac tgccttgatg atattttcaa
   132601 aggcttcttt gattatgacg cgctgaatgc ctattcaatg aagatgaccc gcgatgccga
   132661 atacgattta gtgcatgaga tggaagccag cctgatggag ttgatgtctt ccagtctcaa
   132721 gcagcgttta actgctgagc cggtgcgttt tgtttatcag cgcgatatgc ccaatgcgct
   132781 ggttgaagtg ttacgcgaaa aactgactat ttcccgctac gactccatcg tccccggcgg
   132841 tcgttatcat aattttaaag actttattaa tttccccaat gtcggcaaag ccaatctggt
   132901 gaacaaacca ctgccgcgtt tacgccatat ttggtttgat aaagcccagt tccgcaatgg
   132961 ttttgatgcc attcgcgaac gcgatgtgtt gctctattat ccttatcaca cctttgagca
   133021 tgtgctggaa ctgctgcgtc aggcttcgtt cgacccgagc gtactggcga ttaaaattaa
   133081 catttaccgc gtggcgaaag attcacgcat catcgactcg atgatccacg ccgcacataa
   133141 cggtaagaaa gtcactgtgg tggttgagtt acaggcgcgt ttcgacgaag aagccaacat
   133201 tcactgggcg aagcgcctga ccgaagcagg cgtgcacgtt atcttctctg cgccggggct
   133261 gaaaattcac gccaaactgt tcctgatttc acgtaaagaa aacggtgaag tggtccgtta
   133321 cgcacacatc gggaccggga actttaacga aaaaaccgcg cgtctttata ctgactattc
   133381 gttgctgacc gcagatgcgc gtatcaccaa cgaagtacgg cgggtattta actttattga
   133441 aaacccatac cgcccggtga catttgatta tttaatggtg tcaccgcaaa actctcgccg
   133501 tctgttatat gaaatggtag accgcgaaat cgccaacgcg cagcaagggc tgcccagtgg
   133561 tatcaccctg aagctaaata accttgtcga taaaggcctg gttgatcgtc tgtatgcggc
   133621 ctccagctcc ggcgtaccgg ttaatctgct ggttcgcgga atgtgttcgc tgatccccaa
   133681 tctggaaggc attagcgaca acattcgtgc catcagtatt gttgaccgtt accttgaaca
   133741 tgaccgggtt tatatttttg aaaatggcgg cgataaaaag gtctaccttt cttccgccga
   133801 ctggatgacg cgcaatattg attatcgtat tgaagtggcg acaccgctgc tcgatccgcg
   133861 cctgaagcag cgggtgctgg acatcatcga catattgttc agcgatacgg tcaaagcacg
   133921 ttatatcgat aaagaactca gtaatcgcta cgttccccgc ggcaatcgcc gcaaagtacg
   133981 ggcgcagttg gcgatttacg actacatcaa atcactcgaa caacctgaat aaccctatgc
   134041 caatacacga taaatcccct cgtccgcagg agtttgctgc ggtcgatctt ggttcaaaca
   134101 gttttcacat ggtcatagcc cgtgtggtag atggtgccat gcagattatt ggccgcctga
   134161 aacagcgggt gcatctggcg gacggcctgg ggccagataa tatgttgagt gaagaggcaa
   134221 tgacgcgcgg tttgaactgt ctgtcgctgt ttgccgaacg gctacaaggg ttttctcctg
   134281 ccagcgtctg tatagtcggc acccatacgc tgcgtcaggc actgaacgcc actgactttc
   134341 tgaaacgcgc ggaaaaggtc attccctacc cgattgaaat tatttccggt aatgaagaag
   134401 cccgtctgat ttttatgggc gtggaacata cccaaccgga aaaaggtcgc aaactggtta
   134461 ttgatattgg cggcggatct acggaactgg tgattggtga aaatttcgaa cctattctcg
   134521 ttgaaagccg ccggatgggt tgtgtcagct ttgcccagct ttattttcct ggcggggtca
   134581 tcaataaaga gaattttcag cgcgctcgca tggcggcagc acaaaaactg gaaactttaa
   134641 cctggcaatt ccgtattcag ggctggaacg ttgcaatggg cgcttccggt accataaaag
   134701 ccgcccatga agtgttaatg gaaatgggcg agaaagacgg gataattacc ccggaacgtc
   134761 tggaaaaact ggtaaaagaa attttacgtc accgtaattt cgcatcgctg agtttaccgg
   134821 gtctttccga agagcggaaa acagtcttcg ttcctggact ggcgatttta tgtggtgtgt
   134881 ttgatgcttt agccatccgt gaactgcgcc tttctgacgg ggcgttacgc gaaggcgtac
   134941 tgtatgaaat ggaaggacgt ttccgtcatc aggatgtgcg tagtcgcacc gccagcagcc
   135001 tcgccaacca gtatcacata gacagcgaac aggcccgacg agtgctggat accactatgc
   135061 aaatgtacga acagtggcgg gaacagcaac cgaagctggc gcatccgcaa ctggaggcgc
   135121 tactgcgatg ggctgccatg ctgcatgagg tcgggttgaa tatcaaccac agcggtttgc
   135181 atcgccactc cgcttatatt ctgcaaaaca gtgacttgcc gggttttaat caggaacagc
   135241 agctgatgat ggcgacactg gtgcgctatc accgtaaagc gattaagctc gacgatctgc
   135301 cgcgctttac cttgtttaag aagaaacagt tcctgccact gatacagcta ttgcgccttg
   135361 gcgtattact aaacaatcaa cgtcaggcaa ccaccacacc gccaacattg acactgatta
   135421 ctgatgacag tcactggaca ctgcgtttcc cgcatgactg gtttagtcag aatgcgctgg
   135481 tactgcttga tctggaaaag gagcaagaat actgggaagg cgtggctggc tggcggttga
   135541 aaattgaaga agaaagtacc ccagaaatcg ccgcttaatg ctttgcgggc cgacgagaaa
   135601 tgtcggcccg cattattcag gcactttcgc gaatgggttc gatttcattc agcgtatcaa
   135661 ttaacggttg cggcttacca ataagataac cctgcatata atcgatcccc aaagagagca
   135721 ccgcctcgcg gatctcttcg ttttcaacgt actctgccac tacccgcatt ttcttcattc
   135781 gcgccagatg gcaaatcgac gccacaatct gataatccag actatttgac acaatattgc
   135841 ggataaagct gccgtcaatt ttaagcagat cggcattcac atttttaagc cgtgcatagc
   135901 tggcgtagcc ggtgccgaaa tcatcaatcg caatctggca gcctaattcc tgaagatgct
   135961 gcaaggtgat ttgcgcctgc ttaacattgg tcagagcatt actttcggtg acttcaaaaa
   136021 taagttgcca cgcttcaatc tgatatttag ccagcaactg actgacttca acaggaaaac
   136081 gagcctgaca taccgaggtt ggagacagat taatagcaaa acggtgagcg ggcatcttcg
   136141 ctctgttttc agccataaat tgcagcgtat gctcaatgac ccacatgtcg atactcgacg
   136201 ataaaccaaa ttcgtgcgcg accggcaaga agctaccggg gccgatcagt tcatcattct
   136261 cacctttcat gcgcagaaga atttcatggt aaacatcacc acgcataccg gtaatcggct
   136321 gggccatcag gaaaaaatgg ttgtgttcca gcgcctgctg tagtcgattc atcatcgcga
   136381 ctttatcttt caattcgcgt tgcaaataca ttgccccgca acgctgcata ttttccgggg
   136441 cgttggtcac gatggaaagt tcggcgaccg tatttagctc tcccagcagc aggtagatat
   136501 gattcactgg cgagcgcaca tagcagtaac tgacgccaat ctgcggttgc atcggcatcc
   136561 catcccaaaa gaaacgaaat tgcttgagat gactatccag tgcggtaatg cgctcctggt
   136621 gcgattctgt attcagtcgc agcgcgagat cgttacccga aagctgataa acatcttcac
   136681 ccggttccag caagggtgac agccagtgag aaagtttttg ctt
//
LOCUS       DAJTRB010000002        95189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTRB010000002 DAJTRB010000000
VERSION     DAJTRB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.002"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(6..1256)
                     /gene="icd"
                     /locus_tag="OO330_000132"
     CDS             complement(6..1256)
                     /gene="icd"
                     /locus_tag="OO330_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5881157.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1428..2081
                     /gene="rluE"
                     /locus_tag="OO330_000133"
     CDS             1428..2081
                     /gene="rluE"
                     /locus_tag="OO330_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5881158.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGTELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2091..2552
                     /gene="nudJ"
                     /locus_tag="OO330_000134"
     CDS             2091..2552
                     /gene="nudJ"
                     /locus_tag="OO330_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5881159.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2606..3712
                     /gene="mnmA"
                     /locus_tag="OO330_000135"
     CDS             2606..3712
                     /gene="mnmA"
                     /locus_tag="OO330_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5881160.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3748..4389
                     /gene="hflD"
                     /locus_tag="OO330_000136"
     CDS             3748..4389
                     /gene="hflD"
                     /locus_tag="OO330_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5881161.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4393..5763
                     /gene="purB"
                     /locus_tag="OO330_000137"
     CDS             4393..5763
                     /gene="purB"
                     /locus_tag="OO330_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5881162.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5932..6603
                     /gene="phoP"
                     /locus_tag="OO330_000138"
     CDS             5932..6603
                     /gene="phoP"
                     /locus_tag="OO330_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5881163.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6603..8063
                     /gene="phoQ"
                     /locus_tag="OO330_000139"
     CDS             6603..8063
                     /gene="phoQ"
                     /locus_tag="OO330_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5881164.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8860..9141)
                     /locus_tag="OO330_000140"
     CDS             complement(8860..9141)
                     /locus_tag="OO330_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881165.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9155..10816)
                     /locus_tag="OO330_000141"
     CDS             complement(9155..10816)
                     /locus_tag="OO330_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5881166.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10800..11126)
                     /locus_tag="OO330_000142"
     CDS             complement(10800..11126)
                     /locus_tag="OO330_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5881167.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11182..11364)
                     /locus_tag="OO330_000143"
     CDS             complement(11182..11364)
                     /locus_tag="OO330_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881168.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11348..11788)
                     /locus_tag="OO330_000144"
     CDS             complement(11348..11788)
                     /locus_tag="OO330_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5881169.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11788..12084)
                     /locus_tag="OO330_000145"
     CDS             complement(11788..12084)
                     /locus_tag="OO330_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5881170.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12081..12419)
                     /locus_tag="OO330_000146"
     CDS             complement(12081..12419)
                     /locus_tag="OO330_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5881171.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12416..13627)
                     /locus_tag="OO330_000147"
     CDS             complement(12416..13627)
                     /locus_tag="OO330_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5881172.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVDQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13629..14201)
                     /locus_tag="OO330_000148"
     CDS             complement(13629..14201)
                     /locus_tag="OO330_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5881173.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDPSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14241..15398)
                     /locus_tag="OO330_000149"
                     /pseudo
     CDS             complement(14241..15398)
                     /locus_tag="OO330_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15699..15923)
                     /locus_tag="OO330_000150"
     CDS             complement(15699..15923)
                     /locus_tag="OO330_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881174.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16065..16226)
                     /locus_tag="OO330_000151"
     CDS             complement(16065..16226)
                     /locus_tag="OO330_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5881175.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16236..16541)
                     /locus_tag="OO330_000152"
     CDS             complement(16236..16541)
                     /locus_tag="OO330_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881176.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16538..16816)
                     /locus_tag="OO330_000153"
     CDS             complement(16538..16816)
                     /locus_tag="OO330_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881177.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17105..18853)
                     /locus_tag="OO330_000154"
     CDS             complement(17105..18853)
                     /locus_tag="OO330_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5881178.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIAILLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18850..19149)
                     /locus_tag="OO330_000155"
     CDS             complement(18850..19149)
                     /locus_tag="OO330_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5881179.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19155..19388)
                     /locus_tag="OO330_000156"
     CDS             complement(19155..19388)
                     /locus_tag="OO330_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881180.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19390..19610)
                     /locus_tag="OO330_000157"
                     /pseudo
     CDS             complement(19390..19610)
                     /locus_tag="OO330_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19603..19824)
                     /locus_tag="OO330_000158"
     CDS             complement(19603..19824)
                     /locus_tag="OO330_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881181.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19814..20014)
                     /locus_tag="OO330_000159"
     CDS             complement(19814..20014)
                     /locus_tag="OO330_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5881182.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20144..20323
                     /locus_tag="OO330_000160"
     CDS             20144..20323
                     /locus_tag="OO330_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881183.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20448..20777
                     /locus_tag="OO330_000161"
     CDS             20448..20777
                     /locus_tag="OO330_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881184.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20827..21015)
                     /locus_tag="OO330_000162"
     CDS             complement(20827..21015)
                     /locus_tag="OO330_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5881185.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21390..22619)
                     /locus_tag="OO330_000163"
     CDS             complement(21390..22619)
                     /locus_tag="OO330_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5881186.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLNLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGVQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22868..23989
                     /gene="roxA"
                     /locus_tag="OO330_000164"
                     /gene_synonym="ycfD"
     CDS             22868..23989
                     /gene="roxA"
                     /locus_tag="OO330_000164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5881187.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24038..25264)
                     /gene="pepT"
                     /locus_tag="OO330_000165"
     CDS             complement(24038..25264)
                     /gene="pepT"
                     /locus_tag="OO330_000165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5881188.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25514..26650
                     /gene="potA"
                     /locus_tag="OO330_000166"
     CDS             25514..26650
                     /gene="potA"
                     /locus_tag="OO330_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5881189.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26634..27491
                     /gene="potB"
                     /locus_tag="OO330_000167"
     CDS             26634..27491
                     /gene="potB"
                     /locus_tag="OO330_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5881190.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27488..28282
                     /gene="potC"
                     /locus_tag="OO330_000168"
     CDS             27488..28282
                     /gene="potC"
                     /locus_tag="OO330_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5881191.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28279..29325
                     /gene="potD"
                     /locus_tag="OO330_000169"
     CDS             28279..29325
                     /gene="potD"
                     /locus_tag="OO330_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5881192.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29481..30302)
                     /gene="cobB"
                     /locus_tag="OO330_000170"
     CDS             complement(29481..30302)
                     /gene="cobB"
                     /locus_tag="OO330_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5881193.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30318..31229)
                     /gene="nagK"
                     /locus_tag="OO330_000171"
     CDS             complement(30318..31229)
                     /gene="nagK"
                     /locus_tag="OO330_000171"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5881194.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31258..32502)
                     /gene="lolE"
                     /locus_tag="OO330_000172"
     CDS             complement(31258..32502)
                     /gene="lolE"
                     /locus_tag="OO330_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5881195.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32502..33203)
                     /gene="lolD"
                     /locus_tag="OO330_000173"
     CDS             complement(32502..33203)
                     /gene="lolD"
                     /locus_tag="OO330_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5881196.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33196..34395)
                     /gene="lolC"
                     /locus_tag="OO330_000174"
     CDS             complement(33196..34395)
                     /gene="lolC"
                     /locus_tag="OO330_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5881197.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLMPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34657..35730
                     /locus_tag="OO330_000175"
     CDS             34657..35730
                     /locus_tag="OO330_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5881198.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFIYTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35858..39304
                     /gene="mfd"
                     /locus_tag="OO330_000176"
     CDS             35858..39304
                     /gene="mfd"
                     /locus_tag="OO330_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5881199.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39447..40409
                     /gene="ldtC"
                     /locus_tag="OO330_000177"
     CDS             39447..40409
                     /gene="ldtC"
                     /locus_tag="OO330_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5881200.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40492..40704)
                     /locus_tag="OO330_000178"
     CDS             complement(40492..40704)
                     /locus_tag="OO330_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5881201.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40989..41621
                     /gene="comR"
                     /locus_tag="OO330_000179"
     CDS             40989..41621
                     /gene="comR"
                     /locus_tag="OO330_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5881202.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41683..42221)
                     /locus_tag="OO330_000180"
                     /pseudo
     CDS             complement(41683..42221)
                     /locus_tag="OO330_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
     gene            complement(42448..43752)
                     /gene="ndh"
                     /locus_tag="OO330_000181"
     CDS             complement(42448..43752)
                     /gene="ndh"
                     /locus_tag="OO330_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5881203.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44160..44702)
                     /locus_tag="OO330_000182"
     CDS             complement(44160..44702)
                     /locus_tag="OO330_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5881204.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44725..45750)
                     /gene="nagZ"
                     /locus_tag="OO330_000183"
     CDS             complement(44725..45750)
                     /gene="nagZ"
                     /locus_tag="OO330_000183"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5881205.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(45761..46585)
                     /gene="thiK"
                     /locus_tag="OO330_000184"
     CDS             complement(45761..46585)
                     /gene="thiK"
                     /locus_tag="OO330_000184"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5881206.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46566..47207)
                     /gene="lpoB"
                     /locus_tag="OO330_000185"
     CDS             complement(46566..47207)
                     /gene="lpoB"
                     /locus_tag="OO330_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5881207.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47222..47599)
                     /locus_tag="OO330_000186"
     CDS             complement(47222..47599)
                     /locus_tag="OO330_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5881208.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47602..47961)
                     /gene="hinT"
                     /locus_tag="OO330_000187"
     CDS             complement(47602..47961)
                     /gene="hinT"
                     /locus_tag="OO330_000187"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5881209.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48300..50485
                     /gene="fhuE"
                     /locus_tag="OO330_000188"
                     /pseudo
     CDS             48300..50485
                     /gene="fhuE"
                     /locus_tag="OO330_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50545..51978)
                     /gene="ptsG"
                     /locus_tag="OO330_000189"
     CDS             complement(50545..51978)
                     /gene="ptsG"
                     /locus_tag="OO330_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5881210.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52273..53070)
                     /locus_tag="OO330_000190"
     CDS             complement(52273..53070)
                     /locus_tag="OO330_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5881211.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53081..54085)
                     /gene="holB"
                     /locus_tag="OO330_000191"
     CDS             complement(53081..54085)
                     /gene="holB"
                     /locus_tag="OO330_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5881212.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLLREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54082..54723)
                     /locus_tag="OO330_000192"
     CDS             complement(54082..54723)
                     /locus_tag="OO330_000192"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5881213.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54713..55735)
                     /gene="yceG"
                     /locus_tag="OO330_000193"
     CDS             complement(54713..55735)
                     /gene="yceG"
                     /locus_tag="OO330_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5881214.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55738..56547)
                     /gene="pabC"
                     /locus_tag="OO330_000194"
     CDS             complement(55738..56547)
                     /gene="pabC"
                     /locus_tag="OO330_000194"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5881215.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56667..57908)
                     /gene="fabF"
                     /locus_tag="OO330_000195"
     CDS             complement(56667..57908)
                     /gene="fabF"
                     /locus_tag="OO330_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5881216.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57995..58231)
                     /gene="acpP"
                     /locus_tag="OO330_000196"
     CDS             complement(57995..58231)
                     /gene="acpP"
                     /locus_tag="OO330_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5881217.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58442..59176)
                     /gene="fabG"
                     /locus_tag="OO330_000197"
     CDS             complement(58442..59176)
                     /gene="fabG"
                     /locus_tag="OO330_000197"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5881218.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59189..60118)
                     /gene="fabD"
                     /locus_tag="OO330_000198"
     CDS             complement(59189..60118)
                     /gene="fabD"
                     /locus_tag="OO330_000198"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5881219.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60134..61087)
                     /gene="fabH"
                     /locus_tag="OO330_000199"
     CDS             complement(60134..61087)
                     /gene="fabH"
                     /locus_tag="OO330_000199"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5881220.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61155..62225)
                     /gene="plsX"
                     /locus_tag="OO330_000200"
     CDS             complement(61155..62225)
                     /gene="plsX"
                     /locus_tag="OO330_000200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5881221.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62306..62479)
                     /gene="rpmF"
                     /locus_tag="OO330_000201"
     CDS             complement(62306..62479)
                     /gene="rpmF"
                     /locus_tag="OO330_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5881222.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62531..63052)
                     /gene="yceD"
                     /locus_tag="OO330_000202"
     CDS             complement(62531..63052)
                     /gene="yceD"
                     /locus_tag="OO330_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5881223.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63251..63835
                     /gene="yceF"
                     /locus_tag="OO330_000203"
     CDS             63251..63835
                     /gene="yceF"
                     /locus_tag="OO330_000203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5881224.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63947..64906)
                     /gene="rluC"
                     /locus_tag="OO330_000204"
     CDS             complement(63947..64906)
                     /gene="rluC"
                     /locus_tag="OO330_000204"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5881225.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65479..68664
                     /gene="rne"
                     /locus_tag="OO330_000205"
     CDS             65479..68664
                     /gene="rne"
                     /locus_tag="OO330_000205"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5881226.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68861..69814)
                     /gene="flgL"
                     /locus_tag="OO330_000206"
     CDS             complement(68861..69814)
                     /gene="flgL"
                     /locus_tag="OO330_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5881227.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(69826..71468)
                     /gene="flgK"
                     /locus_tag="OO330_000207"
                     /pseudo
     CDS             complement(69826..71468)
                     /gene="flgK"
                     /locus_tag="OO330_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71534..72475)
                     /gene="flgJ"
                     /locus_tag="OO330_000208"
     CDS             complement(71534..72475)
                     /gene="flgJ"
                     /locus_tag="OO330_000208"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5881228.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72475..73572)
                     /gene="flgI"
                     /locus_tag="OO330_000209"
     CDS             complement(72475..73572)
                     /gene="flgI"
                     /locus_tag="OO330_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5881229.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73584..74282)
                     /gene="flgH"
                     /locus_tag="OO330_000210"
     CDS             complement(73584..74282)
                     /gene="flgH"
                     /locus_tag="OO330_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5881230.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74335..75117)
                     /gene="flgG"
                     /locus_tag="OO330_000211"
     CDS             complement(74335..75117)
                     /gene="flgG"
                     /locus_tag="OO330_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5881231.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75255..76006)
                     /gene="flgF"
                     /locus_tag="OO330_000212"
                     /pseudo
     CDS             complement(75255..76006)
                     /gene="flgF"
                     /locus_tag="OO330_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76026..77234)
                     /gene="flgE"
                     /locus_tag="OO330_000213"
     CDS             complement(76026..77234)
                     /gene="flgE"
                     /locus_tag="OO330_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5881232.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77259..77954)
                     /gene="flgD"
                     /locus_tag="OO330_000214"
     CDS             complement(77259..77954)
                     /gene="flgD"
                     /locus_tag="OO330_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5881233.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77966..78369)
                     /gene="flgC"
                     /locus_tag="OO330_000215"
                     /pseudo
     CDS             complement(77966..78369)
                     /gene="flgC"
                     /locus_tag="OO330_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78373..78789)
                     /gene="flgB"
                     /locus_tag="OO330_000216"
     CDS             complement(78373..78789)
                     /gene="flgB"
                     /locus_tag="OO330_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5881234.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSFDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78944..79603
                     /gene="flgA"
                     /locus_tag="OO330_000217"
     CDS             78944..79603
                     /gene="flgA"
                     /locus_tag="OO330_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5881235.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            79679..79972
                     /gene="flgM"
                     /locus_tag="OO330_000218"
     CDS             79679..79972
                     /gene="flgM"
                     /locus_tag="OO330_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5881236.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79977..80393
                     /gene="flgN"
                     /locus_tag="OO330_000219"
     CDS             79977..80393
                     /gene="flgN"
                     /locus_tag="OO330_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5881237.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80433..81968)
                     /gene="murJ"
                     /locus_tag="OO330_000220"
     CDS             complement(80433..81968)
                     /gene="murJ"
                     /locus_tag="OO330_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5881238.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82135..83001)
                     /locus_tag="OO330_000221"
     CDS             complement(82135..83001)
                     /locus_tag="OO330_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5881239.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(83003..83650)
                     /locus_tag="OO330_000222"
     CDS             complement(83003..83650)
                     /locus_tag="OO330_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5881240.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLYSLLAHLGD"
     gene            complement(83661..84245)
                     /gene="rimJ"
                     /locus_tag="OO330_000223"
     CDS             complement(83661..84245)
                     /gene="rimJ"
                     /locus_tag="OO330_000223"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5881241.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84481..85689
                     /gene="mdtH"
                     /locus_tag="OO330_000224"
     CDS             84481..85689
                     /gene="mdtH"
                     /locus_tag="OO330_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5881242.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85753..86400
                     /gene="grxB"
                     /locus_tag="OO330_000225"
     CDS             85753..86400
                     /gene="grxB"
                     /locus_tag="OO330_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5881243.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86534..87094
                     /locus_tag="OO330_000226"
     CDS             86534..87094
                     /locus_tag="OO330_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5881244.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87200..88246
                     /gene="pyrC"
                     /locus_tag="OO330_000227"
     CDS             87200..88246
                     /gene="pyrC"
                     /locus_tag="OO330_000227"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5881245.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88320..88565
                     /gene="dinI"
                     /locus_tag="OO330_000228"
     CDS             88320..88565
                     /gene="dinI"
                     /locus_tag="OO330_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5881246.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88855..89109
                     /gene="bssS"
                     /locus_tag="OO330_000229"
     CDS             88855..89109
                     /gene="bssS"
                     /locus_tag="OO330_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5881247.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89224..90342
                     /gene="solA"
                     /locus_tag="OO330_000230"
     CDS             89224..90342
                     /gene="solA"
                     /locus_tag="OO330_000230"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5881248.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90390..90503
                     /locus_tag="OO330_000231"
     CDS             90390..90503
                     /locus_tag="OO330_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5881249.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90764..91330
                     /locus_tag="OO330_000232"
     CDS             90764..91330
                     /locus_tag="OO330_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5881250.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91334..91909
                     /locus_tag="OO330_000233"
     CDS             91334..91909
                     /locus_tag="OO330_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5881251.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91951..93003)
                     /locus_tag="OO330_000234"
     CDS             complement(91951..93003)
                     /locus_tag="OO330_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5881252.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93228..94148
                     /gene="lpxL"
                     /locus_tag="OO330_000235"
     CDS             93228..94148
                     /gene="lpxL"
                     /locus_tag="OO330_000235"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5881253.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94320..94517
                     /locus_tag="OO330_000236"
     CDS             94320..94517
                     /locus_tag="OO330_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5881254.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94541..>95189
                     /locus_tag="OO330_000237"
     CDS             94541..>95189
                     /locus_tag="OO330_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5881255.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTL"
BASE COUNT        22873 a        25240 c        23508 g        23568 t
ORIGIN      
        1 agtgattaca tattcttgat gatcgcatca ccaaactctg aacatttcag cagtttagcg
       61 ccttccatca gacgctcgaa gtcataggtt acggtcttgg cgttgatcgc gccttccata
      121 cctttaacaa tcaggtctgc ggcttcagtc cagcccatat ggcgtaacat catctcagcg
      181 gagagaataa tagagccagg gtttactttg tcctgaccgg cgtatttcgg cgcagtaccg
      241 tgggtggctt caaacagggc gcattcgtca ccgatgtttg ctccaggggc gataccgata
      301 ccgccaacct gcgctgccag agcgtcagaa atgtagtcac cgttcaggtt catacaggcg
      361 ataacatcat attcagccgg acgcagcagg atctgttgca ggaatgcatc agcaatcacg
      421 tctttaatga cgatctcttt gccggtattc gggtttttaa ctttcagcca cgggccgccg
      481 tcgatcagtt caccgccaaa ttcttcacgc gccagctggt agccccagtc tttaaacgcg
      541 ccttcggtga acttcatgat gttgcctttg tgcaccaggg tcacagagtc acgatcgtta
      601 gcaattgcgt attcgatcgc tgcacgaacc agacgtttgg tgccttcttc agaacacggc
      661 ttaataccga taccgcaatg ttccgggaag cgaattttct tcacgcccat ctcttcacgc
      721 aggaatttaa tcactttctc ggcgtcggca gagtcagctt tccattcgat acccgcataa
      781 atgtcttccg agttttcacg gaagataacc atatcggtca gttcagggtg tttaaccggg
      841 cttggagtgc cctgatagta acgtaccgga cgcaggcaga tgtagagatc cagttcctga
      901 cgcagggcaa cgttgagaga acgaataccg ccaccaaccg gagtggtcag cgggccttta
      961 atggcaacgc gatattcacg aatcagatca agggtttcag caggcagcca gacatcctga
     1021 ccataaacct gtgtggattt ttcaccggtg taaatttcca tccaggagat tttacgctcg
     1081 cctttatagg ctttctcgac tgcagcgtcg accactttca gcatggctgg ggttacatct
     1141 acaccgattc catcaccttc aatgtaaggg ataatcggat tttcaggaac gttgagtttg
     1201 ccgttttgca gggtgatctt cttgccttgt gccggaacaa ctactttact ttccattcac
     1261 ctctccttcg agcgctactg gtttgctcgt ctgccaccac gttgcatatg cgtttgcgtc
     1321 ctgcgatacg gatgctttag agcaattttt tgttaatgat ttgtaattga cttgtcaata
     1381 ctaccctatt gtttgccgtc atgaaagatg cgcgttatta ggctataatg cggcaattca
     1441 taatctctga aaataccatg caaaaaactt cttttagaaa tcaccaggtt aagcgattca
     1501 gctcgcaacg ttctaccagg cgtaaacatg aaaaccagcc cacgcgtgtg atcctcttca
     1561 ataaacccta cgatgttctt ccgcagttca ccgatgaaac cggacgcaaa acattaaaag
     1621 aattcatccc ggttcagggc gtttatgcag caggtcgcct tgaccgcgat agcgaagggt
     1681 tgctggtgct gaccaataac ggcgcgttgc aggcgcgttt aacccagccg ggtaaacgca
     1741 ccggaaaaat ctattatgtg caggtggaag gtattcccac acaagacgca cttgaagcct
     1801 tgcgcaatgg cgtaacctta aatgatggcc ctaccctgcc cgcaggcacg gagctggttg
     1861 acgaacccgc gtggttatgg ccgcggaatc caccaattcg tgaacgcaaa agtattccca
     1921 ccagctggct gaagatcacc ttatatgaag gacgtaatcg ccaggtgcgc cgcatgaccg
     1981 cccatgttgg tttccccacg ctgcgactga ttcgctatgc gatgggagat tactctttgg
     2041 ataatcttgc caatggcgaa tggcgagaag tgacagatta aggatgcaaa atgtttaaac
     2101 cgcacgttac cgttgcttgc gtggtgcacg cagaaggcaa atttttagtc gttgaagaga
     2161 cgattaatgg taaagcgtta tggaaccaac ctgccgggca tctggaagcc gatgaaacct
     2221 tagtggaagc cgccgcccgt gaactgtggg aagaaaccgg catcagcgcg cagccgcaac
     2281 actttattcg tatgcatcag tggattgcgc cagataaaac gccgtttttg cgtttcctct
     2341 ttgccattga gcttgagcaa atatgcccga cccagcctca tgacagcgat atcgactgct
     2401 gccgttgggt cagcgccgaa gaaattttac aggcgtcaaa tcttcgttcg ccgctggtgg
     2461 cggaaagtat tcgttgttat caaagcgggc aacgttatcc gctggagatg attggcgatt
     2521 ttaactggcc ttttacaaag ggtgtcatct aaaaggctgc gtgatagaat acgccgcctt
     2581 gaagttcaat gtcgtgagtg atccaatgtc tgaaaccgca aaaaaagtaa tcgtcggcat
     2641 gtccggcggt gtcgattcct ccgtttctgc ctggctgttg caacaacagg gatatcaggt
     2701 cgaaggcctg tttatgaaga actgggaaga agacgacggt gaggaatatt gcacagcggc
     2761 agcggatctg gctgatgccc aggcggtctg cgacaagctc ggcattgaac tgcacaccgt
     2821 taactttgct gccgagtact gggacaacgt cttcgaactg ttccttgccg aatataaagc
     2881 cggtcgcacg ccgaatccgg atattctgtg caacaaggag atcaaattta aagccttcct
     2941 cgaatttgcc gccgaagatt taggtgccga ttatatcgct accggtcatt acgtacgtcg
     3001 ggccgatgtc gatggcaaga gccgcctgct gcgtggtctg gacagcaata aagaccagag
     3061 ctacttcctt tatacgctca gccatgagca gattgcgcaa agcctgttcc cggtaggcga
     3121 actggaaaaa ccgcaggtgc gtaagattgc tgaagatctt ggtctggtca ccgcgaagaa
     3181 aaaagactct accggcattt gcttcattgg cgaacgtaaa ttccgcgagt tcctgggccg
     3241 ttatctcccg gcgcaaccgg gcaaaatcat taccgtcgat ggcgatgaaa ttggtgagca
     3301 ccaggggctg atgtatcaca ctctcggtca gcgtaaaggt ctgggtatcg gtggcaccaa
     3361 agaaggcacc gaagaaccgt ggtatgtggt ggacaaagac gtcgaaaaca acattctggt
     3421 tgtcgctcag ggccatgaac acccgcggct gatgtctgtc gggttgattg cccagcagct
     3481 gcactgggtc gatcgcgaac cattcaccgg cactatgcgt tgcacggtaa aaacccgcta
     3541 tcgccagacc gacattccgt gcaccgtcaa ggcgctggac gacgatcgca ttaaagtgat
     3601 tttcgatgag ccggttgccg ccgtgacgcc gggccagtct gccgtcttct ataacggtga
     3661 agtgtgcctc ggtggcggta ttattgagca gcgtctgccg ctgccggtct gattattatc
     3721 tttacttaaa acaaggaagc agtgaacgtg gcaaagaatt actatgacat caccctcgcc
     3781 ctggccggta tttgtcagtc ggcacgcctg gtgcaacaac tcgctcacca ggggcattgt
     3841 gatgccgatg cgctacacgt ctcactcaac agtattattg atatgaaccc cagctcgacg
     3901 ctggcggttt ttggcggtag cgaagccaac ctgcgcgtcg ggctggaaac cctgctcggc
     3961 gtgctcaatg ccagcagttg ccagggctta aacgccgaat taacccgcta cacactcagc
     4021 ttgatggtgc ttgagcgcaa actctcctca gcgaaaggcg cgctcgacac tctgggcaac
     4081 cggatcaacg gcctgcaacg ccagctcgaa cacttcgatt tacagtccga aacgctgatg
     4141 agcgcgatgg ctgctatcta tgttgatgtg atcagcccgc ttggcccgcg cattcaggtc
     4201 accggttccc ctgctgtact gcaaagccca caagtgcagg cgaaagttcg cgcaaccctg
     4261 ctggcaggca ttcgcgccgc cgtgctctgg caccaggtcg gcggcggacg tctacaactg
     4321 atgttttctc gtaatcgcct gaccactcag gcaaaacaaa ttcttgctca tttaaccccg
     4381 gagttgtgat ctatggaatt atcctcactg accgccgttt cccctgtcga tggacgctac
     4441 ggcgataaag tcagcgcgct gcgcgggatt ttcagcgaat atggtttgct gaaattccgt
     4501 gtacaagttg aagtacgttg gctgcaaaaa ctggccgcgc acgcagcgat caaggaagtt
     4561 cctgcttttg ctgccgacgc aatcggttac cttgatgcaa ttgtcgccaa tttcagcgaa
     4621 gaagatgccg cacgcatcaa aaccatcgag cgtaccacta accacgacgt taaagcggtt
     4681 gagtatttcc tgaaagaaaa agtggcggag atcccggaac tgcacgcggt ttctgaattc
     4741 atccactttg cctgtacttc ggaagatatc aataacctct cccacgcatt aatgctgaaa
     4801 accgcgcgtg atgaagtgat cctgccgtac tggcgtcaac tgattgatgg cattaaagat
     4861 ctcgccgttc agtaccgcga tatcccgctg ctgtctcgta cccacggtca gccagccacg
     4921 ccgtcaacca tcggtaaaga gatggcaaac gtcgcctacc gtatggagcg ccagcaccgc
     4981 cagcttaacc aggtggagat cctcggcaaa atcaacggcg cggtcggtaa ctataacgcc
     5041 cacatcgccg cttacccgga agttgactgg catcagttca gcgaagagtt cgtcacctcg
     5101 ctgggtattc aatggaaccc gtacactacc cagattgaac cgcacgacta cattgccgaa
     5161 ctgtttgatt gcgttgcgcg cttcaacacc attctgatcg actttgaccg tgacgtctgg
     5221 ggttatatcg cccttaacca cttcaaacag aaaaccattg ctggtgagat tggttcttcc
     5281 accatgccgc ataaagttaa cccgatcgac ttcgaaaact ccgaagggaa tctgggcctt
     5341 tccaacgcgg tattgcagca tctggcaagc aaactgccgg tttcccgctg gcagcgtgac
     5401 ctgaccgact ccaccgtgct gcgtaacctc ggcgtgggta tcggttatgc gctgattgcg
     5461 tatcaatcca ccctgaaagg cgtgagcaaa ctggaagtga accgtgacca tctgctggat
     5521 gagctggatc acaactggga agtgctggca gaaccaatcc agacagttat gcgtcgctat
     5581 ggcatcgaaa aaccgtacga gaagctgaaa gagcttactc gcggtaagcg cgttgacgcc
     5641 gaaggcatga agcagtttat cgatggtctg gcgttgccag aagaagagaa agcccgcctg
     5701 aaagcgatga cgccggctaa ctatattggt cgagctatca cgatggttga tgagctgaaa
     5761 taagcctcaa atcagtgccg gatggcgatg ctgtccggcc tgcttattaa gattatccac
     5821 tttttctttt ttcactttac ctcccctccc cgctggttta tttaatgttt acccccataa
     5881 acacataatc gcgttacact attttaataa ttaagacagg gagaaataaa aatgcgcgta
     5941 ctggttgttg aagacaatgc gttgttacgt caccacctta aagttcagat tcaggatgct
     6001 ggtcatcagg tcgatgacgc agaagatgcc aaagaagccg attattatct caatgaacat
     6061 ttaccggata ttgcgattgt cgatctcgga ttgcctgacg aggacggtct gtcactgatt
     6121 cgccgctggc gtagcaacga tgtttcactg ccgattctgg tattaaccgc ccgtgaaagc
     6181 tggcaggaca aagtcgaagt attaagtgcc ggtgctgatg attacgtgac taaaccgttt
     6241 catattgaag aggtgatggc gcgaatgcag gcattaatgc ggcgtaatag cggtctggct
     6301 tcacaggtca tttcgctccc cccgtttcag gttgatctct ctcgccgtga attatctatt
     6361 aatgacgaag tgatcaaact gaccgcgttc gaatacacca ttatggaaac gttgatacgc
     6421 aataatggca aagtggtcag caaagattcg ttaatgctac aactctatcc ggatgcggag
     6481 ctgcgggaaa gccataccat tgatgtactg atgggacgtc tgcgcaaaaa aattcaggca
     6541 caatatccgc aagaagtcat taccaccgtt cgcggccagg gctacctgtt cgaattgcgc
     6601 tgatgaaaaa attactgcgt ctttttttcc cgctctcgct gcgggtacgt tttctgttgg
     6661 caacggcagc ggtagtactg gtgctttcgc ttgcctacgg aatggtcgcg ctgatcggtt
     6721 atagcgtcag tttcgataaa actacgtttc ggctgttacg tggcgagagc aatctgttct
     6781 atacccttgc gcagtgggaa aacaataagt tgcatgtcga gttacccgaa aatatcgaca
     6841 agcaaagccc caccatgacg ctaatttatg atgagaacgg gcagctttta tgggcgcaac
     6901 gtgacgtgcc ctggctgatg aaaatgatcc agcctgactg gctgaaatcg aatggttttc
     6961 atgaaattga agcggatgtt aacgatacca gcctcttgct gagtggagat cattcgatac
     7021 agcaacagtt gcaggaagtg cgggaagatg atgacgacgc ggagatgacc cactcggtgg
     7081 cggtaaacgt ctacccggca acatcgcgga tgccaaaatt aaccattgtg gtggtggata
     7141 ccattccggt ggagctaaaa agttcctata tggtctggag ctggtttatc tatgtgctct
     7201 cagccaatct gctgttagtg atcccgctgc tgtgggtcgc cgcctggtgg agtttacgcc
     7261 ccatcgaagc cctggcaaaa gaagtccgcg aactggaaga acataaccgc gaattgctca
     7321 atccagccac aacgcgagaa ctgaccagtc tggtacgaaa cctgaaccga ttgttaaaaa
     7381 gtgaacgcga acgttacgac aaataccgta cgacgctcac cgacctgacc catagtctga
     7441 aaacgccact ggcggtgctg caaagtacgc tgcgttctct gcgtagtgaa aagatgagcg
     7501 tcagtgatgc tgagccggta atgctggagc aaatcagccg catttcacag caaattggct
     7561 actacctgca tcgtgccagt atgcgcggcg ggacattgct cagccgcgag ctgcatccgg
     7621 tcgccccact gctggacaat ctcacctcag cgctgaacaa agtgtatcaa cgcaaagggg
     7681 tcaatatctc tctcgatatt tcgccagaga tcagctttgt cggtgagcag aacgattttg
     7741 tcgaggtgat gggcaacgtg ctggataatg cctgtaaata ttgcctcgag tttgtcgaaa
     7801 tttctgcaag gcaaaccgac gagcatctct atattgtggt cgaggatgat ggccccggta
     7861 ttccattaag caagcgagag gtcattttcg accgtggtca acgggttgat actttacgcc
     7921 ctgggcaagg tgtagggctg gcggtagccc gcgaaatcac cgagcaatat gagggtaaaa
     7981 tcgtcgccgg agagagcatg ctgggcggtg cgcggatgga ggtgattttt ggtcgccagc
     8041 attctgcgcc gaaagatgaa taaatatgtc cttttaccac tacatcaagt cattgattcg
     8101 atcaaacaat tttcattgaa aaagtgccaa aaatcgcaaa ttgactacac cattaactac
     8161 accgttcggt gcactgtatg aaacaacgtg gaacaaatag acacaagaaa tatacaggcg
     8221 ggtaatcttt ccagggggaa gcgcaccaat tcatgagggg cgttaatgtc gatatgggga
     8281 tccccataac ggggctaccg gatttttttt ccggttaacc tttaatcagg ctggtgggtt
     8341 ttacataccc ttgatcaccg taatgatgat ccagatgtgc accttttcca ccaatggcgg
     8401 aaaagctcca accgtaacga tgatcgctat ggttgcctcg atggtctgcg tggtcattat
     8461 gaccgttcag ccagtgacct taatcagaat ttttcgctaa aaaatgacgt tggccacgtc
     8521 atccggaaag cacaaaatca cgtcgatttt taatgaacgg ttaaaccgtc tttttgtcac
     8581 ggtgtgtcac tggttgtcac tctccgtcac gattgaggtg gtggtcattc tgcccgaccg
     8641 cgcatttttg cgctatcggg aatatcaaac agttacctcg ccgacttcct caaattgagg
     8701 tttccgaaaa tatcagagcg ggttagcgat taagctgacg agtaattaaa ctgcgaaaat
     8761 ttcgtagttt ctgaatatat tccggcctgc tggttaacag tcctcagatg tgagggatgt
     8821 agccagcgca attttgcgct ttgctttaat ctcagtgagt taccgccgta accgctccgg
     8881 cttcttccag tggtacgtga ttttctcctt ctcccgatac agttcaacgc ggcgattgta
     8941 ggccagcatt tccagaacgc ggatccgtat gtcgcgcata tccgcatcat taagctggat
     9001 accatcacgg cgcatcacct cagcaacaac acgcgcataa ttttcggctg tcacgctgtc
     9061 cggctgcgtg gtctgttcgt cagcctgctg gctgattcca gagacgcggc ggattaatcg
     9121 cagtatttcg gcttctgtca tgctgctgac ctcattacac cccgctaaat tcttccagtt
     9181 tctggcggtg gctgtcgctt atatcaaaag caaaatcctc atgctctgcc tggaatgtac
     9241 caaacgccat cagcgccgct acgctcgggt ctatcttgtt cggtgatttt ttcttgttcg
     9301 gcttgatatt ggcgttcgcg tcactctgca tcacgacgtt actcatcgac caggacaaca
     9361 ccggatcgcc acgatgcaca atcaccctgc ggttaacaaa aacttcgaac gatttcgccg
     9421 ccggactgaa tctgaggtag gtttgcggga acggctccac ctcaaaacca gccccctgta
     9481 attgcgtcct gagatgcgtg gcgttccacg tatcaaagcc caccagcctg atattaaatt
     9541 tctccgcatc ctgcatgatg tcatctctga tccggtcgta atcaatgcag tcacctggcg
     9601 ttgtgcgtat ccagcccgct ttagcccact ggcgatagac agcgcggttt ttattggcgg
     9661 ggttctgtag ctggaactcc ggcagataat gacgggaaat cagcataatg gttttaccga
     9721 ccggaaaggc atagcatacg ctggaaatat cgctggttga tgataagtcc agccccgcgt
     9781 aacactcctg accgtgtaaa tcttcctccg tgaacgttcc ggcacactca gcccatgcac
     9841 cattacccat ccacggggta gtcccctgac accagatatt aaatcgcttt gtcatcatct
     9901 ccacccactg cgacggaata ccccgcgctt tctggatggt tgaggccagt ttttcacgat
     9961 ccacggaaac atcaatatta gggttcgcct ttatccacat cgccggatca tcaacctcgt
    10021 tttcgtcgtc cagttcgtaa atcagtacaa aaattgaatc gttgacctct tcgccgtcca
    10081 ggatctggca gcaataatca tagtgctgtt tacaggctga aacgacgtta ctgcccgatg
    10141 tggtaatggc aaataacagc ccttcgggac gtgcgcccat ccccagctca agcgcggaat
    10201 aaacgccgtt atcggggtgt aggtggtatt cgtccacgat ggaaagactg gggtttgtcc
    10261 cctcgatggt tgccgctttt gctgccagcg gctttaacag gctgttgctt ttcgggtgta
    10321 tcaccttatg cgcctgaata ttcacccgcc tgcgtaacgg tcgggataaa aggcacatct
    10381 gacgcgcatt atcaaacacg atccgcgcct ggtcacggct caccgctgcg gtgtagatat
    10441 cctgctgacc gttctccata atcagaaacc agttagccag aatcgcggcg gtcgtggatt
    10501 tcgcattttt gcgcggcact tcgataaagg cgctcgtgta tttgcgccgt ccggtggcct
    10561 taaccttaaa gccgaggatg cacgcaaagg cgaactgctg ccacggctcc agctcaatgg
    10621 gtctgccacg catcggtcct tttacgtgcg ggcacaccct ggaaaaggca ataaaccgct
    10681 ccacaacctc acgatcgaac gtgtaaaggg ggcttttaag gtccgaaaag taccgtttaa
    10741 cagcctgttt taaccgttta caggccggaa ttttgcccgt ttttacgtct tctgcgtact
    10801 tattccaggc ggtcaagctc gtcctcttct tctgtttccg gtggattttt acggcggctt
    10861 atcgggtcaa aaccgagcaa ggaggccatt tttatcatca ctctttcagc gtcggatttt
    10921 gcgcttaatg cggggtttct gctctcgccg ccctgactgt taacaatgct gaacccgcgc
    10981 gccgcaaggt ctgcaacggc tttccggtaa atggagtagt tgacgcaata cagttccaga
    11041 ttgctccagt cggcgggggt caggtctccc cgttccgcaa gctgccgcga tttttcccgc
    11101 cactgcttca cggcgatatc atccaggtag gcggggtcgg gtttgtcctg aaaacgcccc
    11161 ccaccccgtc atacagcctc atcagcgatt gcggaaacat tccataacct cgcggtcacg
    11221 gtcggttaat cgcttcgctg tgatgcgttc tgcgcgtcct gacgctttat ctttatgccc
    11281 tgtttcctgt gtcttccatg cgtcacgctg ccttataagt ccacggataa ggcggttttg
    11341 ttcccgctca ttcatcagcg tcatacatcc agttattgcg gttagcagcc cgttcttcct
    11401 cttcacgaaa tccacctgcg gcacgcttgc tttttgttgc cggatcaagc catttcgttt
    11461 tctggttatg acacgcctgg cacaatggtt gatggttcca ttcgggccag aagagaacat
    11521 catcaccgcc attaatcggg ataatgtgat ccaccaccac ggcgggcgta tatatcccct
    11581 tctcaaggca tcgcacgcat aacgggtttt tactcagata catggcgcgg tatttgtccc
    11641 actgtctgga atatccacgc gcgcggcggt gtcctcgtct ggcatcctct gcacgccatg
    11701 ccgcccgcct gtgctcctca cacttgccgg atttaacgcg cctgttacag cctggttctg
    11761 tgcatcgtct taatggttgc cacggcatca gtaccccccc acatcacggt aagccgtcca
    11821 gagtgcgcct atcgtcatgg gtacgcgtgt ttttgcgtta tccgcgacaa tctggcgatt
    11881 ttcatacagg tgagcgataa acatcatgca gccaatcttt atggctggcg tgaacgccag
    11941 cccgtcatca aagcgcctgc ctatgtgtat ctggcacgcc tcaagcgatg cggcaatgta
    12001 tccgctgatt aactggtctt catcgtcgcc atcgatgcgg caatggagtt tcacttcttc
    12061 cagggtgata agttcttctg tcatttttcc gcgccctcac gacaaagaat ttcaaggcgt
    12121 gtcctggcgg catccggcag cggctgcccg atgatattca gtacacgccc cgccagcggc
    12181 cccgtactga cctttatccg gctggtggcg ttgatgtcct tccggtaccg tatccagatc
    12241 cttacggtac caatcgccag ctctgcgccc gatgaaatgg cctccttgct gctgaccatg
    12301 ttcacgcttg cccagagtgt gtgaccgtcc tcccacgttt cgagtatttc gccggttata
    12361 tccctggtct gtttcagggt ctgaatcgtt atcctgtcac gcagtcgccc tatgttcatt
    12421 ccggtttttc tccctcgctg atttttactt cctgtttcca tgcctggctg aactcatcac
    12481 caccatcacg cggtgacagc ccttcacgtt cgcgggcttc gttcggacac ataacgtcgg
    12541 attttatgcc gcgctcatag gtggcaaagc gttcgccagg tgtggcccgt aacaggtccg
    12601 cgctgtcaaa ctccacctga taccggatac caggtacagg cgaggccacc agcaaggcgt
    12661 tttttatctg ctgctcaaag ttcgccagcc acgggcgcat cgtcatggtg agaaatgcgc
    12721 ggcttgcctc gctgaagttg ctgtaggtgc tgttgctgta ttcctgcaaa aaaatcggcg
    12781 agacgttgaa cattcgggcg atgtcttcaa tggtgaagcg acgggaggcc agccattcgg
    12841 catcctgatt actcatgccc agctgctgat agctcatccc cccttcaagg atgggcgttt
    12901 ttccggcgtt tctggcccct ttgtagcgtt ccagtgcggc taatgcctgt ttgcccttca
    12961 cgccgtccag ccattcgcct gacgtgataa cccctgccgc catcatgcca tcacgcatca
    13021 cgctcgcgcc gtggcgttgt tgggccagcc caagccccag cgattcgcgg cagatggtta
    13081 caggtgagcg ccccataaaa ccgtcatccg tggagtaacg aaggtggaga acctcccacg
    13141 gtaaatagtt gcgggtgttt ccggtgtagg cgtcagtgat gcagtagcgc cagttatgat
    13201 cacctgtctg atccacgttc accgactgcg gcgggtaagg atgtaaagcc gccggaaaac
    13261 catcacgccc ccactgaata accgcataag cattaccact taacaggcag tagcggatca
    13321 tcattcgctt aaactggtag ggtgtctgcc acgcgttcgg gcgctcgttg aggatgtgat
    13381 cgaccggatg agaatcaagc cattcgcggg cctccttccc cttctcattg cgtaccaggt
    13441 acaggtaaca cggcatggta gccaccgcgt cagagatgac cgtgacggcg ttcatgacgg
    13501 cggggagtga ttccgctgtc ccagatgata cgtactcacc cgcccccgtg ttcgatgtgc
    13561 cggccagcgc cataaattca tcaagcgtca tgctgcgctg ctcttttttt cgtctgaaag
    13621 gccacatatc acacccccgc taaatccgcc caccagcggc gattatccgc acgcggcatt
    13681 ttttcggggt gctgctcata cagggaacgg cgggccagct ccacgccgga atcggggtaa
    13741 gccggtaccg atgtaacggt aatttcgtac agttccgcca ccagcacggt gcgcacgcat
    13801 ggatctgttg tggtatccca tacatcctta cgggagcgaa agccaaagct catccccgag
    13861 atgtccccac gcttaaccag ttcgataacg tcgcgccctg tactgggatc cggtggggtc
    13921 agttcaaagc gtaaccctgt ttcgtcctct tccagtttca gcgtgccgga acgggtgcgc
    13981 cccagtaaca tgctgtggtc atgctcatac aggccgcgaa cgtcattacc cgccgcaagc
    14041 cattcagtaa acgccccccg ctggaatttt tcgtaaaact caccccataa cagttctgaa
    14101 agcttatccc agcgaacaac gtagcccgtc agcgtaccgg ctccgctggt ggtgatttcc
    14161 gatgaccgga tttccatact cttcataatt ttttcaccca taaaacacta aaggggcttt
    14221 ttagcccctt ctgtatgctg ttaatcgtcg tcttccggca gtgccagaat cttgatcgcg
    14281 ttcgaatcca ccacgccacc gcctaaatat ttttgcgtga aaattttgat gaatcctggt
    14341 tctgtcaggt tgtccggtct ggtgcgaaca cctgtttcgt gatcaacaat gtagtaaccg
    14401 cgtttgaagt cacccagggc aataacgtta tcaggcataa actccagata ttcgaccgga
    14461 aggcccagca acgtatcagg atcacccgcc tgtaaacggt cgcgccagat ataatcaccg
    14521 ttcgcgttct tcaccttctg aagttttgcc gccgtcgtgg agttaaccac tcagaccgcg
    14581 tttttgcggt attttttacg taatgcaaat ttcaggtcga tcagcgggtc cgcagatgtc
    14641 cacgccagag attcggaagg tttaattacc tgcaacgtac caaaatcacg ctctttgtcg
    14701 ttcttctctg cacggggtac ggataaaaaa ccttttgctt ttttgtcacc gtcgcccaca
    14761 accagatcgc tttcttcggt ttccgtgaag gtgtcgccaa tctcacccgt cagccatgaa
    14821 aggatgtcca catcggaaaa atccacgatt tcctgtgtgg tgcgcgggta cgcatagacc
    14881 ggatacagct taatgctcac ctcgttaatc tgcggggtgc tggtctgttc gcgtgcctta
    14941 ccctcttcac cgtggttaac ggtcgcaccg ccagcggaaa caagctgctt aaactcgttg
    15001 ctgctgattt tcttcacggt acagatgcga cgcatggtgg attcatccgc cagcattcgc
    15061 atgatttcgg tgttcagttc agggataacg gtataaccac catcagcggg aacgcctgta
    15121 cttaatgcgc gggtttcacc ggtcagaatg tagttgcgta gttctgcggg gtcagtggtc
    15181 tggctgcttt tacctggctt gctgcgctct tcgtctgcaa tggcttcaag gcgggaaatg
    15241 tctttatcga gggattcagc tttagcgcgt aattcgtcaa attttgtgcc ctcagcatcg
    15301 ttaagactgc ggttttcttt ttcagcgttc tccagcatgt cgcgcatctg atttttaatg
    15361 gcggttttct gctggcgtaa ttcgattatt ctctgcataa aaaaagtcct ggggttaagt
    15421 aaggaactcc aggacgcggc aaacactcaa ccgtttttca taaggaaatc agtaatcgca
    15481 ccgatcgttt tcccgcctgg taatgaatat tggcgagcac attaacaggc gggaaagtgg
    15541 ccccagcgtc ctggcaccac gggcgagaat aatcatgatt cagttcgggt aaaatatgcc
    15601 gatccgctca gtgaacaacg tggaacaacc acgaacaaat aatttacaaa aaataacaaa
    15661 aagccggatt gctccggctg tatgtgctgt atgggtgctc agtctctgat ccctttcaga
    15721 aattctatca atgcatctgt ctgttcagga tttactgcca gcatttcacc ggatagcgcg
    15781 caacgaacaa aaccatgatg atccttttca atcagcgccc ccgtttccag aaatgcgcgg
    15841 taatcagtga tattcatcgt ctccatgttg tcagcatggt atttatcacg ctgtttaagt
    15901 atctcatcaa atttcatcgg cattgcgttt tcctctgttg tgtctgtttg tttcagatag
    15961 taactatgcc ggaccgtgac gaaaagccag tagtgctcca taccgtttca aatgacgcaa
    16021 aaaaagccgg attgctccgg ctgcgtggtg tggttctgat attcctactc tgctaatttg
    16081 ctcagcccca tgtctgcgta agtgcgattt actgcatttc tcaggtctgc gtagttctcc
    16141 gttggcggct ccggtgtgcc cttgctgctc tgttttacca gcgggtcgcc caccagccgc
    16201 ccgtaagctg caatttgtgc tgtcatattg ccacctcagt gaagcgatcc ggcattgttc
    16261 tttttcatct cttccatcat tttttcagtg agcattacgc actgaaaccc catgtttttg
    16321 atgtctacgg gtttcatgcc tttgtcagtg gatatcacag gcagaatatc ctgatacaca
    16381 tcgcggtcag ggtaactaag cacacaaagc gcccatgatg ccacgggtga ggatccttcg
    16441 tctgtttcaa cccaccagcc tggtgtagct gcgcttattt gcagaatcgt gatccgtccg
    16501 ttgtgcttgt aaaatttttc accaaatttt ggttcgctca tgattccacc tctccggttt
    16561 taacgttgat tgttgttacc tgttccgctt cggcaatctc ccgctctgtc agcgtggcaa
    16621 aatttgccgc cgtcgtggtc atgaatgcgc ttatcagttc gggatgtgct ttcgcgtatc
    16681 cttcccccgc gtggcggtct atcgttctga ttgccacctt taaagcgtgc tctgtcatgt
    16741 ctaacgcgcg atatttcggt tctgttctgt ctctgcgttt tttgaatgat ttattaaatt
    16801 tccctgaagt gtgcatattt atttttaccc cctcgtttaa aaagttttga gttgtgcctc
    16861 cccttgtcta ccttatctac cttagtggcc ctcatgccag taatggcgct gctttcagcg
    16921 gggtagagtg cttttatcca ctatctaccc cgtgtctacc tccctgtctg attcaggtaa
    16981 aatcaggtag agagggtaga tagtgggtag acagtaaaaa aaggctatct acctaactta
    17041 atgcactgaa ttaaatgtat ttttctttac tcaggtagac agggtagaca gcaattacaa
    17101 aaaattataa aaacgcgtcg cactcgtctg ttgttattgc gttagtctgc gttactccct
    17161 taactttccg cgtaatatat tcatgtccgt aaactttcgc ggctggcttc atagccttgc
    17221 caaagtcatt tacgtttagc ggtttgctcc tgcctgcgta cgccataaac gccagataga
    17281 cgcggtaaag gctgtttctg gtcgtgtact tcactgaatc gccaccgccg cccatcatca
    17341 ggccgcgtgc ttcctccaga aaattcagga actggcaaaa ctcaataacc ggatccgtct
    17401 gttgctttat tgccagagct tcatcaccgt cacgctgttc cagtagtaaa gcccgtgctt
    17461 tctcagagtc ggtaaagttc gccagcaatc ggcggataat gacagggatt tcagccgcaa
    17521 tcttttccgg tagctccctg tctttttcgg cctcactaac gatattgtcg aaacggaaaa
    17581 tcacgcgacg acgtgccaca cctccggccc gttcggtgaa tatcatcggg ttattgttgg
    17641 tcgccagcac caccgccctg attacagccg tgaaacgctt ttcgtatttc gggttaattt
    17701 ccacggggtc accgcccgtg attttcttga tgcccgttcc ttcgcctgta tatttcggct
    17761 ggtcagccag gacgataaga cgactcccga caacctgcgc acgtccacca gcatcatcaa
    17821 gcgatgtcat ttcagcgctt accgtgttct gtttccctgc cagaaggatg gctatgtgtg
    17881 tgaatgtact tttaccgctc ccgccgtctc cggtggcctc aataaacatc tgccagtcgt
    17941 accggttcgc cataatcatg tacagcgcgg cacatatacg catcatcttg cgctggtctt
    18001 ttccggctgc gtgctcaagc catttatgaa agtttagcgc gttatcgcgg atgttctccc
    18061 ctggtgctgg tggcgtgtac tcaatgccgt tgtgcgtggt gatccagttc tccggcgtgt
    18121 gcggggaaaa ttcccccgtt ttcaggtcaa gcgcaccatt ggcgaacggc agcaaatcgc
    18181 cggacggctc gcccattggt tcggcaataa cttttaacgc ttccacggcg ttattgatta
    18241 cgcgcttgct gaaagtggcc ctgtgctctg aatagatcgc caccatttcg cggctaagtt
    18301 ccattgtgct gaccggacac cataccccgc cgcgccatac gtgaacgatt tcactttcct
    18361 gatgcacaca aacgccatca aagcgctcgg caagcagctg cgcgcgctca ctgtctgcca
    18421 tttgtgccag ttgcgtattt ttctgctccg gcatggtaag ccctgcggca atattttcac
    18481 gctcagcatt cagatagcgc cgccagtttt cacacttctg gacgtgcatt ccttcggggt
    18541 aaaaatttgc gtcctgaata tctgccgccg ccagcttctg accaatcttt ttggtctcca
    18601 ccaaatccag ttctccggcc tggtacagcc ttacgcgttt ttttccatcc ggaacaattt
    18661 tcagtgcatc aagttcggca agctgatttg gcccaagcca cacaggcggc acattatcgc
    18721 cggatgcggg gccatcctgc tcctgccatt gtttcgcgtg cgaccaggca tcactacccg
    18781 caaagataat tacctctgtg tctttgtgtt ttattccgcg tggctgtttt tttacgttcg
    18841 gtgccagttt cattttttac ccctgaatac gttaagcatc ttttttattt cctgaatatt
    18901 ggcgcgtgct ttctccctgc tggttggttt actgcggggc gctgcctgta ccagcgtgaa
    18961 atcacgccgg aattgataaa caggcatcac gcaatcatat tcgtaacctt cacggcggta
    19021 ggtgatgcgc cgttctgcca cgcctttaat cgttaccatg ccgccgtact ggtcgcggta
    19081 aatatcaccg cgcgtaaatt tagggtgagt gttgccactg gcagttaagc cagaatattt
    19141 aagtttcatt atttttattc tccggtgtgt tgttctttat atctgtcgtg caatagatct
    19201 atttcttgca gttccattat tacaggctca agaagcgtta ttaatgccgt ggcaattctt
    19261 gatttttgtt tgtcgcgttc attgtcgcca agtgtttcaa gccatatgcg caatatttcc
    19321 agcatgtttt cactgtgaga aagtgcaaga aatgcgcggt ctattgtttc gtggtaaata
    19381 tcacgcatat taatccccgt ccgtcgtttt tcttaaaata acctctgtca cgaaatcagc
    19441 ataatcggcg gcgatattca gtacatcaag cccgttgatt tatattctct cgtggaaagt
    19501 aagaaaaaag ccgctctaat aagttctggc attgacgaaa gcgcatcagc cgcatcatca
    19561 ggaacgccgg aaaattcctg tttcagggaa ttaaaacgat catcacgcat aacccccccc
    19621 attttcacaa tcggcaacaa gaatattttt agcgtcattc agtgaccgcg ttgcgatgta
    19681 tcggatacat tccagggcga actgtgtccg gtctttctgt tcttctccct gcgcaagctc
    19741 tgccgtgcat tcaatatcaa taagcgcgtg catcagcgta gtgagtgcgg cggctgctgc
    19801 gttcggtgtg gttttattgt gcataggttt ccccctgacg aatacgggcg gcgaatacag
    19861 caacacaacc ggacgggcaa cggctacgcg cttcgcgttc cgtccaagcg gttacgtgga
    19921 tgatttggga ttctccggca ctcagtgcca gaaaacgcca cacaaaggcc gtttgtgtgt
    19981 gtacaaggtg tggtatatga tttacggcaa ccataacggc tcctagttta cgttgttggt
    20041 tagaagcccc gttactgctc ctacagtgcg gggctttgct ttttgtgttg attgtgtgta
    20101 atgtgtcact acacataaat acattacctg aggtgtaatt aacgtgtcaa cacacaaaaa
    20161 cgaaaggcgt ggtaatcctc cgttccagtt ccgtttagat cctgacttac gggaaaagat
    20221 ggaaaaggca caacaacagg acggcgatga ttctcttgct gcctggataa agcgaataat
    20281 ccgcaaagaa ttacagcaac gcggcataga acccaaagga taaaaactat cagcgccgtg
    20341 gtgtgaggta ctacggcgca ttgctttaca ggcgcacaca atgaccaacg aagaatcaac
    20401 cgaaaaaaca tcacctgcac gaaaaagacg acgcagaaag aaagagcatg aatcagaacg
    20461 attcgcgccg tgttcttttg cccttgagaa gtaccttaaa gaatactcat caaaggagag
    20521 aacttcgcag ttatggcaac gcactgaacc agactgattg cattgcccac cagcctgata
    20581 gcggctatca tccccgtgct tatgtttggg atcacacaca caaacggcgc agcgggttat
    20641 ctgttcagaa aggcggctcc atttcggggc cgcttttttt atgcctgaaa aaccccaatt
    20701 ttgtggtttt ccagtttcac cagggcgaac gaatccccgc ccacgttctg gcgtatattc
    20761 aatcttcatg gttatagctc tgtgttcaga tgattgatgt gtggcggctg cgtgccgcca
    20821 gcgtgattaa tgaactgcct tgcagctatc cttccaggcc agaacctcgg ataaagacca
    20881 gccaacggaa cgaccgccaa gtttacgacg tgatgggaat tgtccggcct tttccaggcg
    20941 gtagcggcat gagcggctaa ggcctgttag cttttcgcat tctttttcac gtataaaccg
    21001 atcagtgctt aacactattg ccccctttcg tttcttaaag agttatttcg tgttctattg
    21061 cgttgggatg tgtctgattg tgtcaggatg attcagagtt ggcaaatgtt gaggccgtat
    21121 ggtttacaga aagaggaata atcaggataa aatcctttaa attcatgtta atacaaaggc
    21181 ataaaatatt gtttcatgcc ttttttctcg ctatttaaag agtgattcgc tagtgtataa
    21241 aaaatcagta acacattaaa aatcagctac ttataaatct gcacactttt tcacctcttg
    21301 ttcgtagttg ttccgctttg ttgctcattg ttgcacgttg tatctgttcg catatccagt
    21361 atgcgcatac tgaaaaaaca cgaaaaaaat tattttcttc tggctactgg tagcgtggtt
    21421 acgttttcat gtgttcccgc cagtatccct aaccgctccg tccacatatc cagcgcattg
    21481 cgtttagcat ccagataacg ggaatgatta taaactcgct gcactcctgg catctggtgg
    21541 cctgtaagct gctccacgac gtgaggatcc acgcctaaat cgttcagcat cgttgtaaag
    21601 gtgcgccgga tgtcatgtag tgaccagtga gggtgattta gcctcctgtg cgctaatctg
    21661 ccgtactgcg acacgcttgt ttcctgtttc acttccccca gcaataagcc cgtgtgcctg
    21721 ttctgctcca ccagctgcgt gacgaacggc agtattgctt ccggtatggg ccggaatatt
    21781 gccaccttcg ttttgctgtg ttctttcggc acggtccaga gcatttcggt aaaatcccac
    21841 tcgctgatct ccgataacct cagttctacc gtccggcatc cgaacacaat caggaggcgg
    21901 attaacgcga tgtagtaggg ggagaatatt tttttgtcca gtgcctgcaa taattcgccc
    21961 agttctttgg tgcttaagac acgctcgctt atatccggtt ttttcccaac gtccgccaca
    22021 ctcatatcat caagaacgtt gctgattgca tagcgccgcc tccggcagaa cttaagcgcc
    22081 tgtttgcacg tctgtagcaa gaatccggca gtaacaggcg ttcgctttgc cacctggtca
    22141 aaacaggcca gccagtgccg tagctcgcat ttatccagcg gcatagcacc aatgtgctgt
    22201 attacgtgat tattaaggcg ctttttcagg gcggcataat ccacgcggtt ttcctttgcg
    22261 tacgactcaa gccagtaggt gagcgcatcg ccaaccgtta ccggctttaa cgcttcctgt
    22321 acggtgtaat taagctcatg acgtggattt ttcccctctg ccagccatgc gcgacactgg
    22381 gcggcttttt ccctggctga tttcaggttc agatcaggat aatttcccag cttaatgcgt
    22441 tccggtggtg ccccccttcc cgttccggcc ctgtaagtga aataccaggt taaaaggcca
    22501 ctggttgaat gcctgacgct caggtttcca ccgtcattaa gaaaggctgt tttttgggcg
    22561 ggtgtgccgt tgattttcct cagctgtgta tcgctcagtt tgttaagtgc tctgctcata
    22621 attttgattc cggtcatcac aattaactac accattaact acaccgatcg ttgcacaaag
    22681 ggctacaacg tgaaacaagc tggaacaagg aaaatcacaa atctgtttat aatcaaaaac
    22741 ataataaaca atctgaaaca ttatgaaacg gcaaaaaaca ctaattgata aaatatgtcc
    22801 atacttcacg cattacgtta agcatccgtt ataatcggtt gcagatacca gcctgtggat
    22861 gcttaacatg gaataccaac tcactcttaa ctggcccgat tttcttgaac gtcactggca
    22921 gaaacgcccg gtggtgttaa aacgcggctt taataatttt attgacccga tctctccaga
    22981 cgagttggcg ggtctggcga tggaaagcga agttgacagt cgactggtca gtcaccagga
    23041 tggcaaatgg caggtcagcc acggcccgtt cgaaagctac gatcatctcg gtgaaaccaa
    23101 ctggtcatta ctggtacagg cagtaaacca ctggcatgag ccgaccgccg cgctgatgcg
    23161 accgttccgt gaactaccgg actggcgtat tgatgatctg atgatttctt tttctgtacc
    23221 tggcggcggc gtcggcccgc atctcgatca gtacgacgtg tttatcattc agggtaccgg
    23281 acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa cagcactgcc cacatccgga
    23341 tctgttacag gtcgatccgt tcgaagccat catcgatgaa gagctggagc ctggtgatat
    23401 tctttatatt ccgccaggat tcccgcatga aggctacgcg ctggaaaatg cgatgaacta
    23461 ttccgtgggt tttcgcgcgc caaatacgcg ggaattaatt agcggatttg ccgattatgt
    23521 gctgcaacgt gaactgggcg gcaactacta cagcgatccg gatgttccac ctcgtgctca
    23581 tcctgcggat gttctgccgc aagagatgga taaactgcgt gagatgatgc tcgaattgat
    23641 caaccagccg gaacacttta agcaatggtt tggcgagttt atatcccagt cacgtcatga
    23701 actggatatc gcgccgccgg aaccgcctta tcagccagat gaaatctacg atgcgctgaa
    23761 acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta ttgcgcattg gcgacgacgt
    23821 gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg gcactggatg cactcgccag
    23881 caacattgcg ctgactgcgg agaattttgg cgatgcgctg gaagatccgt cattcctcgc
    23941 gatgctcgcg gcgctggtca atagcgggta ttggttcttc gaagggtaag tttggattta
    24001 agccggatgc agcatcacat gccgcatccc ttttcgctta cttccgttgc gccgttaact
    24061 cggcaatacg gacgatcacc tgcaccgctt tttccatacc ttccagaatc acaaactcat
    24121 gcttaccatg atagttgtaa ccgccagtga acaggttcgg gcaaggtaat cccataaacg
    24181 acaactgcgc gccgtcggta ccgccgcgga tcggtttcag ttccggttca atatcgcagt
    24241 cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg ctcaaccact ttctcgcgca
    24301 tattgtagta actgtcttca atcaccaatt caatgtagca atcaggatgt aaccctttgc
    24361 ccactttttt ggcgatctcc atcattttac gtttacgcgc ttcaaactgt ttacggtcga
    24421 aatcacggat gatgtagtgc atatcggccc gttcaacggt gcctttcatg ctcgccaggt
    24481 gatagaaacc ttcatagcct tctgtcattt ccgggctttc atccgccgga acttccgcat
    24541 gaatacgtgc cgccagcgac agcgcattta ccatcactcc tttcgccgtg cccggatgaa
    24601 cattgttacc gacaattttt atattgaccg atgcggcgtt gaagttttca aactccagtt
    24661 cgcctacgcc accaccgtca acagtgtaag cccagcgggc atcgaaggca tcaacatcaa
    24721 aatgtttcgc ccctttgccc acttcttcat ccggggtaaa ggcgacgcga atatcaccat
    24781 gcggaatgtt tttctgttgc aataccgcca gcgcggtcat gatttctgca atacctgctt
    24841 tgtcatcagc acctaacaag gttttaccat cggtggtaat caacgtctga cccagtagct
    24901 gatgcagcac cgggaacata accggtgata aaacttcatc gccgataccc agcgcaatat
    24961 cgccaccgcg atagttttca acaatttgcg gattaacatt tttgccgctg caatccggtg
    25021 aggtatccac atgagaaata aagccaatcg ccgggatatc gccagggacg ttagccggta
    25081 acgtcgccat caaagtgccc ttctcactta aggtcacatt gataagcccc atttcttcga
    25141 gctgctcttt cagcagatgc aataacttcc attggccttc cgtgctggga acctgtctca
    25201 cccctgcttt tgattgggta tccagagaca cgtagttcaa aaatcgctca agtagtttat
    25261 ccatgtagtc accctcactt tttgtgacaa cattattaag aagcaagaaa agacaaatat
    25321 tgcgtcaggt cacttttacc cctgcaagcg ggaatattta tcagcattac cttaatgaat
    25381 ataaagctaa gccagtaatt cacaacaaag attggcgatt caagcggttt gccgtagaat
    25441 taccgccctc attaagagtc accaaggtgg ttaaccacaa accccgcatc ggtaagccat
    25501 ccgttgcgtt tacatgggac agagtaaaaa attgaataaa caaccgagtt cgctttcacc
    25561 gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt aaagaggtca ttccccagct
    25621 ggatctgact atcaacaatg gcgagttcct cacgctgctt ggcccttctg gctgcggtaa
    25681 aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt gattccggac gcatcatgct
    25741 ggataacgag gacatcaccc acgttccggc ggaaaaccgc tatgtgaaca ctgttttcca
    25801 aagctatgca cttttccccc acatgaccgt gttcgaaaat gtggcctttg ggttgcgcat
    25861 gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg gaagccctgc ggatggtgca
    25921 gctggaaacc ttcgctcaac gtaaaccgca tcagctctct ggtggtcaac agcaacgcgt
    25981 cgccattgct cgcgcggtgg ttaacaagcc tcgtctgttg ttgctggatg agtcgctctc
    26041 agcgctggat tacaaactgc gtaagcaaat gcagaacgag ctgaaagcgt tacagcgtaa
    26101 gcttggcatt acattcgttt tcgtgactca cgaccaggaa gaagcactca ccatgtcgga
    26161 caggattgtg gtgatgcgcg atggtcgcat tgagcaagac ggcacgccgc gtgaaatcta
    26221 cgaagagccg aaaaacctgt ttgttgccgg gttcattggc gaaattaata tgtttaacgc
    26281 cacggtgatc gagcgactcg acgagcagcg cgtacgcgcc aacgttgaag gccgcgaatg
    26341 taatatctac gttaacttcg ccgttgaacc ggggcaaaaa ctgcatgttc tgctgcgccc
    26401 ggaagactta cgtgttgaag agattaacga cgacaaccac gctgaagggc tgattggtta
    26461 cgttcgtgag cgtaactaca aaggcatgac gctggagtcg gttgttgaac tggaaaatgg
    26521 caagatggtg atggtcagcg aattcttcaa tgaagacgat cctgactttg accactctct
    26581 cgaccaaaaa atggccatta attgggtaga aagctgggag gtcgtactgg ctgatgaaga
    26641 acacaagtaa gttccagaat gtagtgattg tcactattgt cggttggctt gtgttgtttg
    26701 tctttctgcc caacctgatg atcattggca ccagcttttt gacccgcgac gacgccagtt
    26761 tcgtcaaaat ggtctttacg ctggataact acacgcgtct gctcgatccg ctctattttg
    26821 aagtgctatt gcactcgctg aatatggcgc tgatcgccac cctcgcctgc ctggtgctgg
    26881 gctatccatt tgcctggttt ctggcgaagc tgccacacaa ggtgcgtccg ctgctgctgt
    26941 ttctgctgat tgttccgttc tggaccaact cattgattcg tatctacggg ctgaaaattt
    27001 tcctcagcac caaaggctat ctcaacgagt ttttgctctg gctgggcgtt atcgacacac
    27061 caatccgtat catgttcacg cccagtgcgg tgattatcgg tctggtttac attctgctgc
    27121 cgtttatggt gatgccgctg tactccagta tcgaaaaact ggataagccg ttgcttgagg
    27181 cggcgcgcga tctcggtgcc agcaagttac agacttttat ccgcatcatt attccactga
    27241 cgatgccggg gattattgcc ggatgtctgc tggtgatgct gccagcgatg ggcctgttct
    27301 atgtgtccga cctgatgggc ggtgcgaaaa acctgctgat cggtaacgtc atcaaggtac
    27361 agttcctcaa tattcgtgac tggccgtttg gtgcagccac cagcattacg ctgactatcg
    27421 taatgggcct gatgttgctg gtttactggc gcgcttctcg tttgctgaat aagaaggtgg
    27481 aactcgaatg atcggtcgac tgcttcgcgg cggttttatg accgctatct acgcgtacct
    27541 gtatatccca atcattattt tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa
    27601 ctggcagggt tttaccacca aatggtatag cctgctgatg aacaacgaca gcctgttaca
    27661 ggcagcgcag cattcactaa caatggcggt gttttcggcg acgtttgcta cgcttatcgg
    27721 ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg
    27781 aatgctgttt gtggtaatga tgtcaccaga tatcgtgatg gcaatttcgc tgctggtgct
    27841 gtttatgctg ctgggtattc agcttggctt ctggtcgctg ctgttctcgc atatcacctt
    27901 ctgcctgcca tttgtggtgg tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat
    27961 gctggaagcg gcgaaagatc tcggtgccag cgaatttacc attctgcgaa aaatcattct
    28021 gccactggca atgccagcgg tggcggcggg ctgggtgtta agctttaccc tgtcgatgga
    28081 cgatgtggta gtttcttcat tcgtcaccgg accaagttat gaaattctgc cgttaaaaat
    28141 ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac gcgctggcaa ccatattact
    28201 ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt gctcgtgata aaacgaaagg
    28261 taacacaggg gacgttaaat gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg
    28321 gcactgggca tgagcgccgc tcacgccgat gacaacaaca cgctgtattt ctacaactgg
    28381 accgagtacg tgccgccagg actgcttgaa cagttcacca aagaaaccgg tattaaggtt
    28441 atctattcga cttacgagtc gaacgaaacc atgtacgcca agctgaaaac gtacaaagac
    28501 ggtgcctatg acctggtggt tccttcaacc tattacgtcg ataaaatgcg taaagagggg
    28561 atgatccaga agatcgacaa gtcgaagtta acgaacttca gcaatctcga tccagacatg
    28621 ctcaacaagc cttttgaccc gaataacgac tactccattc cgtatatctg gggtgcgacg
    28681 gcgattggtg ttaacggtga tgcggtggat ccgaaatctg tcaccagctg ggccgatctg
    28741 tggaagccag agtacaaagg cagcctgctg ttgaccgacg atgcccgtga agtgttccag
    28801 atggcgctgc gtaagctggg ctactccggt aacaccaccg atccgaaaga gattgaagct
    28861 gcatataacg agctgaaaaa actgatgcca aacgtcgcag cgtttaactc cgataacccg
    28921 gctaacccgt acatggaagg cgaagttaac ctcggcatga tctggaacgg ttctgctttc
    28981 gttgcacgcc aggcgggtac gcctattgac gtggtgtggc cgaaagaagg cggcatattc
    29041 tggatggaca gcctggcgat cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg
    29101 atcaacttcc tgctgcgccc ggatgtggca aaacaggttg ctgaaactat cggttatcca
    29161 acgccaaacc ttgcggcgcg taagctgtta agtccagaag tggcaaacga taagacgctc
    29221 tacccggatg ctgaaaccat taaaaatggc gaatggcaga atgacgttgg cgcagccagc
    29281 agcatttatg aagagtatta tcagaagctg aaagcaggac gttaattcag caagcaggcg
    29341 gtggtattac cgcccgtttt ttagttagca aggatgtggc taatgagtca ggacagtaaa
    29401 gttttatccc gctctttcgt cggtattgtg gtccgcttgt aggcctgata agcgtagcgc
    29461 atcaggcaat gcttcctgct ttataatccc tccagcaact tttcaacaaa ttctggcacc
    29521 acctggcttg ccgggccgta atatttctcg gcaaattcat taccaacctg actcggttca
    29581 agattcagtt caacggtgtg agcaccatgt agtttggctt cgtgtacaaa cccagccgcc
    29641 ggatagacat gcccggaagt accaatggca ataaagacat ccgccatcga caacgccata
    29701 taaatttcat ccatgccgag tggcatttcg ccaaaccaaa cgacgtgcgg gcgcaagggt
    29761 gccgggaact ggcaacaatg gcatttatct tctggcgtaa cgtctccggt ccagtcgaga
    29821 acctgaccac tttgcgaaca acgaactttc agcaattccc catgcatatg aatgacattg
    29881 gtattacctg cgcgttcatg caggttgtct atattctgcg ttaccagcaa aaagcgatcg
    29941 ccgagagcgt cttgcagttt agccagcgca agatgcgcgg cgttaggctg aatttctggc
    30001 tgctgcagct gtcgacgacg ggcgttataa aacgcttgca ccagttcagg atcgcgatcg
    30061 aaaccttccg gcgttgccac atcttcaacc cgatgttctt cccacaggcc atctgcggcg
    30121 cgaaaggtac gaatacctga ttccgcagaa attcctgccc ctgtcagtac gagtactctt
    30181 ggtttttcca ttgcttccgg caccacttta tctctgaaaa aaatacgctg acgcaaacgc
    30241 tcgcgcaggc gacgtttatt tttacgaaaa cgacttaacc gatgaccccg acgcgacagc
    30301 atagcaacct ctgttgttta atcggttaga tgtaggaagg ccgcaccacg cattcctccc
    30361 gcatcaccgt ggcgcgcgcg ttcaatgcgc ggaacacgag ctacaggtaa gagatgacaa
    30421 ggcaacctgt ccgccagttg cgttgtgatt gccgggaaat tcgataagcc accaccaatg
    30481 acgaccaggt cagggtcaac aatggtcagg atatttccca gagaaaccgc taataaatcc
    30541 agataacgct caacgtgcgc ccttgcctgc tcatcgcctt gatcataaag cgcaataatt
    30601 tcgggagcct gcaacggttg atgataatag tgttgataca gccacgcaaa accgcgacca
    30661 gacagataat tttcaatgca gccatgctga ccacagccgc agcggcgtaa cgggaaatcc
    30721 aaccccatca tggttaacgc atcaaccggc agacgcatat ggccaaactc gccggtaatg
    30781 tagcttttac cagtaatcgg tttgccgttg aaaaccagcc cgccgccaac gccggtgccg
    30841 agaattaacc ccatcaccag tggatattgc gtaaattcat catcccaggc ttctgaaagg
    30901 gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg
    30961 gcacgcagcg gtttgccgct ggcagcgggg acattagcgg catacagctt accatcttct
    31021 gtttccggca ttcccggaat accgatgccg acagaacctt tacagccaaa acgttggtca
    31081 gcttcagcta ccagctcaca cactgcatct aaaaatgcgt catagctgtc acgcggtgtc
    31141 ggcacccgct tttcccactg caactgccga ccgctatcaa acacgccaag cgcaattttt
    31201 gttccaccaa tatcaaaccc gtaatacatt gccgctcctt gttttaatgt actgccttta
    31261 ctggccgcta aggactcgcg cagggtcaat attactggcg cgccgcgccg gataccaact
    31321 tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag aagacgtcca gccagtgcaa
    31381 ttccgatggc aggaagtcaa taaaataaat atcgctggag aggaactgat gaccgatcag
    31441 cttttcaatc cactcaataa tcggggtaag ttgcagtgaa acaactacgc cgataaccac
    31501 cccacacagg ctgccgaaca gccctgccag caatccatac cagacaaaga tggcgcgaat
    31561 taaaccatct ttcgccccca gcgttcttaa tactgcgata tcgccactct tgtctttcac
    31621 cgccatcact aaggtggaga cgatgttgaa acaggccacg ccaatcacca gtaccatcgc
    31681 cagatacata atggcgcgga tcatctggat atcgcgatac atatagccgt aagtaccaat
    31741 ccaactttta atataaacat agctgttggt cacttcaccc gcatcgcgta ccagcttatt
    31801 ggcgttgaaa acatccgtca ttttaagggc aatacctgac acgctggaac ccatatcaag
    31861 atattgttgg gcatccgcca gcgggatcat ggcaaaactg tgatcgagtt gaccactcaa
    31921 ctgcaaaata ccggcaatgt gcaaacgcac acgttttggc tgcatcagtt tatgctcggg
    31981 attcgagttg gggatcatga tcgacaccca atcgccctgc ttcactttca gcgcatccgc
    32041 cacgcctttg ccgatgataa tttgctgttc gcccgcttta aaattgcgcc acgcatcccc
    32101 ctgaacaaac gagggtaatg cgctcagacg ctgttcctgt tgtgggttaa cgcccttcac
    32161 ctggattgcg cgcagattcg ctccactttc caccagaccg gtgaaattga tatacggcgc
    32221 agcggcggca atacccggca ctttctgcac gttatccagt gcttcctgcc agttagtcca
    32281 tggctgattt accgcttcga tttcgccatg cggcaccacc gccagaatgc ggttattcag
    32341 ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc aataccgcca cgccgagggc
    32401 aatgccaatg gtagaaatga cagagattag cgacaccatg ccgccgcgtc gccgtccgcg
    32461 actaaaacgc aggccaatca ataacgataa aggcatcgcc attactccgc ccccatcagg
    32521 ctcagttccg ccgtcagacg tccatcacgc atttccagtt ggcggctcat acgtttcgcc
    32581 agttgcaggt cgtgagtgac caccaggaaa gcggtgccct gcaagcgatt caactcccca
    32641 agcaactgga agatgctgtc ggcgttacgc gcatcgaggt taccggtagg ttcatccgcc
    32701 agtaccagac gcggattatt gaccagcgca cgggcaatcg ctacacgctg gcgttcgccg
    32761 ccagaaagtt cagatgggcg gtgattcgca cgatgctcca gccccaccgc ttttaacatc
    32821 tccagtgcac agctgttgat ttcagcgggc tttttcttgc caatcagcag tggcatagcc
    32881 acgttttcca gggcagtaaa atccggcagc aggtggtgaa actgataaat aaagcccagc
    32941 ttctggttgc gcagttctgc tttcgccgct gaagacagtt tgctcatcgg ctgaccgtta
    33001 aagatcacat cgccggaggt tggcgtatcc agcccgccta acaggtgcag caaggtactt
    33061 ttaccggaac cagagctacc gacaattgcc atcatttcac cttcgcccac gctgaaactg
    33121 acattgagca gcacatcggt ttgcacactg ccttcctgat agcgtttgca caggttgtcg
    33181 cattgcaaca ggatcttatt cataacgtaa agcctcagcg ggttgtgtgg cagcagcgcg
    33241 ccatgaaggg taaagcgtag acagcagcgc gatagccatc gccaccagcg caataacaat
    33301 gacctgtaaa ggttcgatag ccaccggcag cgccgcgcca tcaagcagga cgccgattat
    33361 cggcatcaga ttatttaact ggctggcaag cagtgcgcca agcgccgctc cgaggattgc
    33421 accgataatc ccggcgctag ccccttgcac cataaagacc atcatgattt gtcgcggagt
    33481 taagccttgc gtttgcagga tcgcgacttc gccctgcttc tccatcacca tcagccccag
    33541 cgaggtaata atgttaaacg ccgcaacagc gacaatcagg ctcagcagta agcccatcat
    33601 atttttttcc atgcgtacgg cctggaacag ctcgccttta cggtcacgcc agtcctgcca
    33661 tttgctgcct tcaggcagtt tttgctgact taaagagtca actctcagcg gctcatccag
    33721 ccacaaacgc cagccggtaa tattggctgc cggataacgc atcagacgcg aggcatcctc
    33781 aatattcacc agcatttcat agccatcgac ttcactgttg gcggcgaaag tgccaatcac
    33841 attgaacagg cgctgacttg gaatacgccc catcggcgtg aactggctgg cagatggcac
    33901 catcacgcgg atttgatcac cgcgattgac gcccaactgt gaggcaagtt gttcgccgag
    33961 gatgacatta tatttccccg gctcgaggtc agtttgtttc acattgacca gatacggcat
    34021 tagtggatct ttttgcgccg ggtcgatacc gagcatcacc ccgaccgcca cgctgcgcgc
    34081 gctttgcagt accacatcac cggtagtaat aggtgcgacg cgattaacgc cgtccagttt
    34141 gactgccgtt tccgggagtt gctgcgggtt aagagagcca tgctcagaag agagaattgc
    34201 ctgtggcatc aggccaagga tgttgttttg cagctcgcgc tcaaagccgt tcatcactga
    34261 caatactgtg accagcgcca tcaccccgag ggtaatgccg atggtagaaa gccaggagac
    34321 gaaacgaccg aagcgatcca ctgcacgccc acgcatgtaa cgcaggccaa taaatagagc
    34381 gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa agacacgagt atataagcga
    34441 aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc
    34501 cgtaaatcaa tcggctaaat tgccatgttt tatcatcaat cttgccgcct gatcttaatc
    34561 tgaaaaatca tccaatacag acagataccc attacacctc gggtcataac cagcaggatc
    34621 aaccgcatgt aaattaaaaa agagcgtgtt gttctgatga aacaaaaaga gctatggatt
    34681 aaccagatca aagggttatg tatttgtctg gtggtgattt atcactcggt cattaccttt
    34741 tatccgcata tgaccacttt tcagcatccg ttatcggaag tcctgagcaa atgctggatc
    34801 tatttcaatc tttaccttgc cccctttcgt atgccggttt ttttctttat atccggctat
    34861 ttgattcgcc gctatatcga cagcgtaccg tggggaaatt gtctcgataa acggatctgg
    34921 aacatcttct gggtgctggc gctttggggc gtggtgcagt ggctggcgtt aagtgcgctg
    34981 aaccagtggc tggcacctga gcgcgattta agcaatgcct ccaatgccgc ttatgccgat
    35041 tccaccggtg agttcctgca cgggatgatc accgccagca ccagcttgtg gtatctgtat
    35101 gcgttaattg tctatttcgt ggtatgtaaa atttttagcc gtctggcgct gccactattc
    35161 gccttgtttg tactgctgag tgtggcggtt aatttcgttc ccacgccgtg gtggggaatg
    35221 aacagtgtga tacgcaattt gccttattac agccttggcg catggtttgg cgcaacaata
    35281 atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc tgatggcttc tttgcttacc
    35341 gttctggcgg tcggtgcctg gttgtttact atctcgctgc tgttgtcgct ggtatcgatt
    35401 gtggtgatca tgaagctgtt ttatcagtac gagcaacgat tcggtatgcg ctccaccagc
    35461 ctgctgaatg tgattggctc caacaccatt gctatctaca ccacccatcg cattctggtt
    35521 gaaatattca gcttaactct gcttgcgcaa atgaacgcag cacgctggtc gccgcaagtc
    35581 gaactgacac tcctgctggt ttatcccttt gtcagtttgt tcatctatac tgttgcgggc
    35641 ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc tgttgttctc cccgccttca
    35701 ctgcccgctg ccgtcagtta ctctcgttaa accatcaccc cagccggggc gattcaattt
    35761 gctaaaccat gtcgattacg gataatgaac agcattgctt atcagagata tgcccccata
    35821 tgttgaggca tatcctaacg agaatctgac aaccgttatg cctgaacaat atcgttatac
    35881 gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag ttaaccggcg cagcctgtgc
    35941 aacgctggta gcggaaattg ccgaacgtca cgccggtccg gtggtactca ttgcaccaga
    36001 tatgcaaaat gctctgcgtt tgcatgatga aatcagccag ttcaccgatc agatggtgat
    36061 gaatctggcg gactgggaaa ctcttcccta cgacagtttt tcgcctcatc aggacattat
    36121 ctcctcgcgc ctttccaccc tttaccagct accgacgatg cagcgtggca tactgattgt
    36181 tccggtgaat acgcttatgc agcgcgtttg cccacacagt tttctccacg gtcatgcgct
    36241 ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta cgaacccaac tggacagcgc
    36301 cggttatcgc catgttgacc aggtgatgga gcacggcgaa tacgccacgc gcggcgcgtt
    36361 gctggatctc ttcccgatgg ggagtgagct gccttatcgt cttgatttct ttgatgatga
    36421 aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc acgctggagg aagtagaagc
    36481 gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa gcggcaattg aactgttccg
    36541 cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg gaacatattt accagcaagt
    36601 gagtaaaggc acattacctg ctgggatcga gtactggcag ccattgttct tcagcgaacc
    36661 actgccgccg ctgttcagtt atttccctgc caataccttg ctcgtgaata ctggcgatct
    36721 ggaaaccagt gccgaacgtt tccaggctga cacgctggcg cgttttgaga atcgcggcgt
    36781 cgatccgatg cgcccgctgt tgccaccaca atcgctctgg ctgcgggtgg acgagctctt
    36841 ctcagagctg aaaaactggc cccgagtgca gctaaaaact gaacatttac cgacaaaagc
    36901 cgcgaatgcc aatttaggtt tccagaaact gccagacctg gccgttcagg cgcaacaaaa
    36961 agcgccgctg gatgcgctgc gtaagttcct cgagactttc gacggtccgg tggtgttctc
    37021 ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg ctcgcacgaa ttaaaattgc
    37081 tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt gggcgttatc tgatgattgg
    37141 cgctgccgaa catggttttg tcgataaggc gcgtaatctg gcgctgatct gcgaaagcga
    37201 tctgctcggt gaacgtgttg cccgccgtcg tcaggattct cgccgcacca tcaaccccga
    37261 tacactgatc cgtaaccttg cggagctgca tattggtcag ccggtggtcc atctggagca
    37321 cggcgtcggt cgctatgccg gaatgaccac gctggaagct ggcggcatta ctggcgagta
    37381 cctgatgctc acctatgcca acgacgccaa actgtatgtt ccggtgtcgt cactgcatct
    37441 gattagccgt tacgcaggtg gcgcggaaga aaacgccccg ctgcataaac ttggcggcga
    37501 tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg cgtgatgtgg cggcggaatt
    37561 gctggatatc tacgcgcaac gcgccgccaa agagggcttc gcgtttaaac acgatcgtga
    37621 gcagtatcag ttattctgcg acagcttccc gtttgaaacc acgccggatc aggcgcaggc
    37681 cattaatgcg gtacttagcg acatgtgtca gccgctggca atggatcgtc tggtgtgcgg
    37741 cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc gctttcctgg cagtagataa
    37801 ccacaagcag gtagcggtgt tggtgcctac cacccttctt gcgcagcagc attacgacaa
    37861 cttccgcgac cgtttcgcca actggccggt acgtatcgaa atgatctccc gtttccgcag
    37921 cgccaaagag cagacgcaaa tccttgcgga agtggcggaa gggaaaatcg atattctgat
    37981 cggtacgcac aaactgctgc aaagtgacgt caagtttaaa gatttaggcc tgctgattgt
    38041 cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc attaaagcga tgcgcgcgaa
    38101 cgtggatatt ctgacgctta ctgcaacgcc gatcccacgt acgctgaata tggcaatgag
    38161 cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc cgtcgtctgg cagttaaaac
    38221 ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg atcctgcgtg aaattttgcg
    38281 cggaggacag gtttattatc tctacaatga tgtggaaaac attcagaaag ccgccgaacg
    38341 gctggcagaa ctggtgcctg aagcacggat tgccatcggt cacgggcaga tgcgcgagcg
    38401 cgaactggaa cgggtgatga atgatttcca tcatcaacgt ttcaacgtgc tggtttgtac
    38461 caccattatc gaaaccggga tcgacatccc gacagccaac accattatca ttgaacgtgc
    38521 ggatcacttc ggtctggcgc agctgcacca gttacgcggt cgcgtcggac gttcacatca
    38581 tcaggcatat gcatggctgc tgacgccgca tccaaaagcg atgactaccg atgcacaaaa
    38641 acgtcttgaa gcgattgcct cgctggaaga tctcggggca ggttttgcgc tggcaacgca
    38701 cgatctggag attcgcggcg cgggtgaact gcttggcgaa gaacaaagcg gctcaatgga
    38761 aaccatcggt ttctcgctgt atatggagtt gctggaaaac gccgtcgatg cactgaaagc
    38821 cggacgcgag ccgtcgctgg aagatctcac cagccagcaa acagaagtcg agctgcggat
    38881 gccgtcgcta ttgccagatg atttcatccc tgacgtgaac acgcgtctgt cgttctacaa
    38941 acgtattgcc agcgccaaaa cggaaaacga actggaagag atcaaagtcg agcttatcga
    39001 tcgcttcggc ctgctgccag atccggcgcg taccctgctg gatattgccc gtctgcgcca
    39061 gcaagcgcag aaactgggga tcaggaagct ggaaggtaat gagaaaggcg gggtgatcga
    39121 atttgccgag aagaatcacg ttaatccggc ctggttgatt ggtttgctgc aaaaacagcc
    39181 gcagcattac cgccttgatg gtccgacgcg cctgaaattt attcaggatt tgagtgagcg
    39241 gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa ctggaagaga acgcgatcgc
    39301 ttaatgtaaa tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa ttctttgcac
    39361 tttcctgcac tatccgacac tgtcaccatc cataattcag gcttatctgt ttattacaat
    39421 aaccttatat ttattatgta tttttggtga tgatcaaaac gcgtttttct cgctggctaa
    39481 cgttttttac gttcgccgct gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc
    39541 cgctgccgcc agcgagcagt cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg
    39601 acggtggttc tctggaagcc atcgccaaaa aatataacgt cggctttctc gctctgttac
    39661 aagctaaccc cggcgttgat ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt
    39721 tgcaaaccct acttccagac gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc
    39781 gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc
    39841 agttaggtgg tgacacgctg acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa
    39901 acccaacctg gacgccaacg gcaaacatcc gcgcccgtta taaagcacag ggaattgagt
    39961 tgcctgcggt agtgccggct ggaccggata acccaatggg ccatcatgcg attcgtctgg
    40021 cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc cgatttcggc attggcatgc
    40081 gggtaagttc tggctgtatt cgtctgcggg atgacgatat caaaacactc tttagccagg
    40141 tcaccccagg caccaaagtg aatatcatca acactccgat aaaagtctct gccgaaccaa
    40201 acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga gaagattgat gacgatccgc
    40261 agctgctgcc aattacgctg aatagcgcaa tgcaatcatt taaagatgca gcacaaactg
    40321 acgctgaagt gatgcaacat gtgatggatg tccgttccgg gatgccggtg gatgtccgcc
    40381 gtcatcaagt gagcccacaa actctgtaaa tcctggataa aaaaaatccc cgcgacacgg
    40441 catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg agggttaatg cttatttata
    40501 aattactgct gttccatgga gggtattcgg accggttaca gaagtaatac ggaaagattt
    40561 tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc agcgatccca gatttgtccc
    40621 cgcgttagca ctgatagtac cgactttttg ttggccttct ggcgttgact gaacttcgac
    40681 agccgcaaag ttggcaaatg acatggagct taaaatcgcc gcagcgatga ggtttttacg
    40741 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    40801 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    40861 attaatttaa aaatctttaa atttcagtca aatagaaagc aattattttt atgggatctg
    40921 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggtaca
    40981 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    41041 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    41101 aagcgacttc tcttgcggac ctcgtcgaag caaccggagc caaagcgccc acgctatacg
    41161 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    41221 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    41281 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    41341 tcatgatcaa caactgcacc accctctccc cagattcagg agatatagcc aatacgttga
    41401 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    41461 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    41521 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    41581 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaggcccctc
    41641 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    41701 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    41761 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    41821 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagata
    41881 gccctggatc tggttaccgg catatccacc acccagcgcc ccacaacagt ggcgacatct
    41941 ttaccgcgac caccaccaaa ctgatgccct atcacgccac cagcaacagc gccgagcacc
    42001 gacccggtaa tgcgattttc atcctgcacc ggtcgacgat gggtcactgt gacgttgcga
    42061 cactcctgac gcggtgtttt aaccgtttcc ttgattgggg ttgcagaaac aacctgagcg
    42121 tattgcgggc cccgttcaaa cacgttcaga ctggccactg ccgctacgcc caacgcagct
    42181 gcgacaccaa tcccgatacc cgccaacatt gatttattca cgggaacacc tccttctttg
    42241 cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta tcagacagaa gatgaaaagc
    42301 gcgggaatga ctggaaatac ttaaggattc ggaggagtct gaaggaatgc ctgatgcgac
    42361 gcttaacgcg tcttatcagg cctaccgctc gtaccatttg tccagggcgg ataaggcgtt
    42421 tataccacat ccgccagtgt acgtcgatta atgcaacttc aaacgcggac ggataacgcg
    42481 gttaatactc cccaccagca tcattaatcc ggttttaaag taaccatgca gcgcaatctg
    42541 atgcattcgg tatagcgaga tatatacaaa gcgcgcaatt cgtccttcaa tcatcattga
    42601 gccgcgcgtc aggttaccca tcaggctacc aacggtggag aagttcgaca gcgataccag
    42661 cgaaccatga tctttatact gataattttt cagcggctta ccgttcatct gcgccagaat
    42721 gttgttcatt gcgcaagtcg ccatctggtg tgcagcctga gcacgcggcg gaacaaagcc
    42781 cccttccgga cgcgggcatg acgcgcagtc gccaatagcg taaatgtctg gatcgcgagt
    42841 ggtttgcagc gtcggttcca ccaccagctg gttgatacgg ttagtttcaa gaccaccgat
    42901 atctttcagg aagtctggcg ctttgatccc ggctgcccat accatcagat cagcctcaat
    42961 atattcgcca tctttagtgt gcaggccgcc ttcatcagca ctggtgacca tggtttgcgt
    43021 cagcacgcga acgccaagtt tcgttagctc gttgtgggcc gcagcagaga tacgtggcgg
    43081 taatgcaggc aaaatacgtt ctcccgcttc taccagcgtt acgttcaggg cttcgttggt
    43141 caggcctttg taaccgtagc tgtgcagttg cttgatcgcg ttgtgcaatt cagcggagag
    43201 ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc actttgccat ttgcgcccag
    43261 gttggcagag tatttcagga acagattcag catctcctgg tggaagcgac gcgcctggtg
    43321 cgggttatcg aggaaaatgc agttctcttt gacgcctggc gtattgaaat cgttagaggt
    43381 gctacccagc gccatgacca gggtgtcata ggcgatttta cgttccggaa ccaacagttc
    43441 acctttctcg tcgcgtagtt ctgcaatagt gattgttttc gcttcacgat caatatcaat
    43501 gacggaaccc aactggaact ggaaaccatg attgcgcgca tgggccagat agctcaacgc
    43561 atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc agcagtggtt tccacaggtg
    43621 gctgtggtta cgatcgacca gcgtaatttt ggcttttttc ttacgtccca gcttatgccc
    43681 cagctgtgtt gccatttcca gcccaccagc accgccgccg acaatcacaa tctttttcaa
    43741 tggcgtagtc aacgtgatcc ccttaaattt attaaccaat tgttaattaa tggttattaa
    43801 catagctctt aattaacaac aggttacagc aatgaaaatg ttcttcgcta ttgagaataa
    43861 catgaatggt gcattggtca taccaaaatt gatgttaatc aagttttgtt gcttaaaaga
    43921 taggcaactt taccggggga gatcaaaaaa tagagtttat tgtttattat ttgagcaaat
    43981 tcggaagggt aatatagggt gaagcgtata tcgagggcgt ttggtcgtcc tgataagatg
    44041 cgcagcatcg catcaggcat tgatgctcga ctgtcggatg cggcgtggac gccttatccg
    44101 acttacagaa ttcggagcgc atcaggtacc ggcaaggtta cctgatgcga cgagggcatt
    44161 tacccgaggg ttttgaacgc tttaatgcgc tgtaaatgcg gggagatatt cttgaatttg
    44221 tgcgtctgct cttcgtccca gacaatctcg taataatgat gcaactcttc agatgtccgc
    44281 tggctgttaa gtgcttcatc attacgcgac aaaataacca gacaacgatc gcgattcttc
    44341 tcgcggaagt tggtcacaca cttagtggca atatcggcat actcttccgg gcgatcaatc
    44401 ttgccttcca tgttctcata agggaacaaa ttagggttga agatcacctg gcggatatcg
    44461 caaagaaaac caatccgttc cgcccagtat ccgcccaggc caacgccgca aattagcgga
    44521 cgctcgtcaa cgttcagttg caacattttg tccacttctt taagcagatg ctgcatatca
    44581 tgtttcggat gccgcgtact gtagcttatc aagcgtacat ccgggtcaat aaactgcaat
    44641 tgtaagactt tctcgtggtt acccggactg ttagagtcaa aaccgtgtaa atagataatc
    44701 atcgcttcct cacataagcc agggttagtg acctgctttc tcttcctgcc agcgttcatg
    44761 taactgattc agacgggtgc tgatcgcttt ccagcgagcc gagtccatca gttcctgtcg
    44821 cgaaaatgaa cctttatgat acaaacgtgt aacacgttct gccttgatcg gtgacagatt
    44881 atctaacacg ctgacggccc ctttacgatt attgcagacc aggatcatat cgcaacccgc
    44941 atccagtgat gcctgcccgc gttcggcata actgcccata atcgcggcac cttccatcga
    45001 taaatcgtca gagaaaatca cgccgtcaaa acccagttcc tgacgcaaaa cggttttcag
    45061 ccagtaggga gaaccgctcg ccggacgcgg atcaacatca ctgtagatca catgcgcagg
    45121 cataatggcg tcgagtttat tttcgcggat taaggaactg aagaccgaca tatctttagc
    45181 gcgaatctcc gcttgtggac gtggatcgca cggtgtttct ttgtgtgagt ctgccgttac
    45241 tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt ccggcttcat gcataccatc
    45301 aataaaccgg ctggcaattg ccagggcttt ttgtggatcg gcatgataag aacgctcgcc
    45361 aatcgccgcg ctgatatgcc cgacatccag cactggcgca aagctgatat caatatccat
    45421 agcgatcatt tcgctggcca tcagccaacc cgcctcttgc gccagtttgc cgccctcttc
    45481 cattcccaac agcgcagcaa aggattgtgc tgccggtaag cgggtaaaac cttcgcgaaa
    45541 acgctgcacg cgtccacctt cctgatctac cgccaccacc agatgattgc gcgatgctgc
    45601 gcggatctgg cgcaccagtt cacgtaactg ggcaggatca tgatagttac gcgtaaagag
    45661 aatcagccct cccaccagcg gatgcgccag tatttcacgc tcttccgcgt ccagttcgta
    45721 accttcgaca tccaacatta ctggacccac actgctctcc ttattgtttt attaatagct
    45781 gccgccaggt gtcatcggcc agcctgataa attgttgatc gccggtttgt cgccagcggt
    45841 actcaaacca ccctcctttg agcatcagca gccagggaaa ccatcgcctg acctgacgcc
    45901 ataattgcgc cggataaatc ttcgcgcgag tggcatagtc attgaccaat tgccggtgct
    45961 gttcagtatt ttccacccac accgccgcca gttccagcgc gatatcacca tctccagcat
    46021 actcccagtc gatgagtttt aaccctgatg cgctatgcac taaatttccg gcgtggacat
    46081 ccatatgcaa tggacttaag cgtaaaggtc gtggttcccg cgctttgcgc agacgtttta
    46141 acattcgcag ccaacccaca gtccgccgcg ccggatcgct ttgctgccag tacagttcca
    46201 gtaagggcaa cagcgttatt cgccagccaa aacgtggttg ttgatgtaga taatacagca
    46261 agcctgccag ttcgttggta tccggcaaat acgtttttac cgcgccgggc agatagtcga
    46321 ctaccatcca gtcacggaga tataaatgcg gcttcggtgc aatgcttgcg ggtagttgtg
    46381 ataaagcccg atactggcgc aagaacgcgg actgcggcgc atcaggatcg tgcggctgac
    46441 gcacaacaaa acgctggccc tgatgttcaa tgagaaaact accgccacta agcccactat
    46501 taaacgtcgt gacgggatga aactgcggga aaaagcgcga cagcaattcg tcgcgcgtga
    46561 tgggattatt gctgcgaaac ggcaccttta cctgaccaga taatttcgcc cgtctgcacc
    46621 agcatcagct gcatttgtag ggtcggagcg ttaacgttgc cagaggcgct ggagtacagc
    46681 acgtaatgag cgccgacatt gcgggcaatg cctatggctt tactacgggt gcctaaactg
    46741 tcctgcggcg acaaacctaa ctgttgctta gccatcgaca gttgctgggc ggaaaccagg
    46801 gtaaatttcc cgttattagc cagcgcattt cgcagcgttt cggtcgcttc tgcggcattc
    46861 agcgaaccgt tagtacggtt gttaacgcta tcaaccagca ggacgctacc cgcagtcacc
    46921 ccgtcagccc caagcatctt actgaccatc ggctgcattg cgccattcca gtcataatgg
    46981 cgaatatgcg gcgcaggcgg tgccgttcga tcttcgtgct caattgggcc tggctgctgc
    47041 gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt gtggctcggc tggttgttcc
    47101 ggcgctggtt tcacttcttc taccggtgca ggttcacgtt gccccacaca cccggcgaga
    47161 aacatcgcca gcgcggtaat caaggcgtag cgactcattt ttgtcatcaa gattcacccc
    47221 tttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
    47281 gcgttaccgc cgaatgtgcg ggaatggtaa cgctgcgtgg cctttccaga ggatgcatct
    47341 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
    47401 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
    47461 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
    47521 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacaccaga gacaccagcc
    47581 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
    47641 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt
    47701 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
    47761 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat
    47821 gagaatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
    47881 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
    47941 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
    48001 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaatct
    48061 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc
    48121 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
    48181 atgcgtatat ttctcatttg catttgcatt tgcagcaaaa ttattcgcac actaaatata
    48241 accgctcatc accctcctcc ggcatgagcc tgacgacaac ataaaccaag agatttcaaa
    48301 tgctttcaac acaatttaac agggataatc aatatcaagt catcaccaaa ccgtcactac
    48361 ttgccggttg catagcactg gcactattac cttctgccgc ttttgctgca ccagccactg
    48421 aagaaacggt gattgttgag ggttcagcca cagctccaga tgatggcgaa aatgattaca
    48481 gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac tcaacgtgat attcctcagt
    48541 cggtcactat tgttagccag cagcggatgg aagatcagca gttacaaacg ctgggcgaag
    48601 tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt
    48661 attcccgcgg attccagatc gataactata tggttgatgg tatccccacc tattttgaat
    48721 cgcgctggaa tctgggcgac gcactttctg atatggcact gtttgaacgc gtagaagtag
    48781 tgcgtggcgc gacaggactc atgaccggga cgggtaatcc atctgcggca attaatatgg
    48841 ttcgaaaaca cgcgacctgt cgtgaattta aaggcgatgt ctcggcggaa tacggtagct
    48901 ggaacaaaga acggtatgtg gcggatttac aaagcccact caccgaagac ggtaaaatcc
    48961 gcgcgcgaat tgtcggcggc taccagaata acgactcatg gctggaccgc tacaatagtg
    49021 aaaagacctt cttctcgggc attgtcgatg ctgatttagg cgatcttacg acgctttcag
    49081 ccggttacga atatcagcgc attgatgtta atagcccaac ctggggcggt ttaccgcgct
    49141 ggaatactga tggcagcagc aacagttacg atcgcgcacg cagtaccgca cctgactggg
    49201 cgtacaacga taaagagatc aacaaggtct ttatgaccct gaagcagcgg tttgctgata
    49261 cctggcaagc gacactgaat gccacccact ctgaagtcga atttgacagc aaaatgatgt
    49321 atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt tgggccatac agtaattatg
    49381 gacctggctt tgatcatgtc ggcggcaccg gttggaacag tggcaaacgt aaagttgatg
    49441 cgctggattt gttcgctgac ggtagttatg aattgtttgg tcgtcagcac aatctaatgt
    49501 ttggtggcag ttacagcaaa caaaacaatc gttcagttca tgggccaaca tcttcccgga
    49561 tgaaattggc agtttctaca actttaatgg caatttccca caagccgact ggtcatcaca
    49621 gagcctggcg caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac
    49681 ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat accaactggc gggttgatac
    49741 gctgacttac agcatggaga aaaaccacac cacgccttac gctggtctgg tgtttgacat
    49801 caatgacaac tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg
    49861 tgacagttca ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgca
    49921 atcggactgg atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga
    49981 taatgtcgct cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa
    50041 agcggtggat gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg caattaccga
    50101 caactggcag ctgacatttg gcgcaacgcg ctatcttgca gaggataacg aaggaaacgc
    50161 cgttaatcct aatctgccac gcaccacggt taaaatgttc accagctatc ggttgcctgt
    50221 catgccagag ttgacagtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac
    50281 cgtgacaccg tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt
    50341 cacccgctac caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga
    50401 caaaacctac gataccaaca tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag
    50461 cattaccggc acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggctgcc
    50521 ttagtctccc caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt
    50581 caggttatcg gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc
    50641 cgctgcgccc agtttcttca ggccagcctg atccacttta gacacatcag caacgctgac
    50701 gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc
    50761 aaccagagcc ggtgccattt cgctggtacc tgttgctttt gcatcttcag tcgcgtcttc
    50821 acgacccggc gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac
    50881 aatcgcataa ccgataccga cgatcggtaa cagccacagt ttgctgctgt taccagacag
    50941 aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat
    51001 acagattggg aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac
    51061 gaacatgaag aagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc
    51121 ggagatcata ataccgccca ctttcgcacg gttttctggt ttagcagagt gccagatagc
    51181 aattgcggca gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc
    51241 agtcgggtca cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt
    51301 ggtgtattca ccaatctgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg
    51361 taccaggcaa cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc
    51421 agcccactga gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac
    51481 aacgccagta aagatggcag ccaggccaga aatgatcggc acaaagcgtt taccggcaaa
    51541 gaagccaaga tactcaggca gcttaatacg gtagaaacgg ttaaacatgt acgctgcaat
    51601 cgcaccggag ataatccctc cgagtacgcc agtatccgcc aagtgtttag aggcgatttc
    51661 ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc
    51721 ataggcaaca actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac
    51781 accgatcgca aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac
    51841 atgcgataca acggcgggca gccagctgaa attcgcggaa ccgacgccca gcagaatacc
    51901 tgcgataggt agtacggata ccggcagcat cagcgattta ccgacctttt gcaggttagc
    51961 aaatgcattc ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgttct
    52021 cacgcgtggc aaggggggag agcctcgccg tgtacagggc atctaagcgc cctttattta
    52081 ttacacagag taaaataatt cagtgccaat ttgtttgacg gctatcacgt ttcaacttca
    52141 gatcattgga gaagttgcat attttgacaa aacatatgtc acaatatttc aaacgcggtt
    52201 attcaccgcc caattttcgg cggtgaactt tactcgtttt agccattaat tacgagcttt
    52261 aaaaaaactc attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc
    52321 gaagttatcg gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat
    52381 gtattctgca acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggtaccgg
    52441 cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc agggggacat aacgcgcagc
    52501 atcgcgcagt tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc
    52561 gagatccagt aatttacccg ccgtttctct gtcctctgta aaacagtgta gtacgccacc
    52621 gcaatccgtc actttttctt cgcgcagaat tgccagcgta tcggcacggg cgtcacgggt
    52681 atggacgata accggcttgt tcagttcacg accaatctgg atatgatgga tgaaggactc
    52741 ttgctgacgt actttagttt ccggtgtgta ataataatcc agcccggttt cacccagcgc
    52801 tacaacaccc tcttctgccg ccagacggcg taaatcttct acatcgtagg gatcattctg
    52861 gttgagtgga tgcacgccac aagaaaatac gacgttgtca cgttcgccta ccagatcccg
    52921 catatgtaaa taacccggta atgttgtggc gactgccaga caaaatttca catcgcgtgc
    52981 ggcggctttc gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc
    53041 gagatggcag tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta
    53101 gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct
    53161 cgcggttgat gcctgtgaca gacattaact gttcacgaat gtggcaaaca tcccccagta
    53221 tagcctgcag gcgcgaggga gaaagatggt ttgccagttc ggcgaccagg cccggcacat
    53281 caacattggt cacctgcgca gcgccatgat ggcgttttag cgcatccatc agcaacgttg
    53341 ccagccagtg taaacgcgcc ggagcttgtt catgattaag ggccgccagc agcgaatacc
    53401 agtcgcccga tggcacgcta tatgccaacg cctgacacaa tgtttcacga gcctgccagt
    53461 tatctccctg aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg
    53521 caagtaatgc atcctgtgac attgtcactt cgcgtaaaag ccaagtcacg gcgtactgtt
    53581 ctggaggcgg cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt
    53641 caggctcgcg ggtagccagg aaaaaccagg tttctgctgg tggctcttca agcgttttca
    53701 gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc ggttacccag acaactttcg
    53761 caccacctag gcgtgcgtgc tcattcagct tttcggtgac ctcacgtacc gcatcaatgc
    53821 ccagcgtatt tttccctttt tcgggagcca gggtgtagta atcgggatgc gtgccagcct
    53881 gcatcaactg acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga
    53941 gtaaataacg gctcagggcg tagattaaag catcatcgcc catgcccggt aacgcctgaa
    54001 tgagtagcgc atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag
    54061 gtcgtaacca tggataccat ctcatgcgtc caactccttc acccagtggg tcacggtagt
    54121 gcggattgca tccatcacgg cctccagcgg ctgggtggca tcaatggtat gaatactttt
    54181 atcttgtgca gccagttcca gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc
    54241 ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt
    54301 aacatcgaga tagagcgtta agtctgggcg aaaatccccg agaacagcat cacgcagtgt
    54361 tgccagcata tgttggtcaa taccacgtcc tccgccctga tacgcctgag tggaaagatc
    54421 gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca ggtttgatga ccgtttctac
    54481 cagttgaacg cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc
    54541 gcctaccgat ttgatatcca gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc
    54601 aggttcccga gtgaaaacca tgtcgcggat acccagttgc tcgagcgtct caaccaccac
    54661 attacgcgcg gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg
    54721 catttttttc cttaagcact ttcagataat cctgcacaga cttgttatga ctggcaagat
    54781 tggtattaaa cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg
    54841 ccggatgcgc agcagccttc agcgaatccg cccccggcgt agctatcgca cccggcggca
    54901 aaccggtaat ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt
    54961 tgccattata acgctctccc atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac
    55021 caatgcgtaa acggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg
    55081 tttctttttc gataattgat gccatcgtca ccagctgatt tttatcttta taaggcagac
    55141 cgtccgcacg cccttcccag gcgctatcga ccgctttcac cattttcttg tgaactcgct
    55201 tgagtaacgc gacatcggtg gtattggcgg tatacatcaa ggtgtctggc cagaaccaac
    55261 cttcaatcca ctccggattt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt
    55321 cactcagcgt gtgcttgata tacggagcct cacgcaattg cttgaggtag tcgctcagac
    55381 gcatcccttc taccagtcgc agcgggaact gtgcttcttt accgctttcc aacaatttca
    55441 gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa
    55501 gatccggttc gatacgcagc agccattgaa aaacccgtgg gcgattgatg atcttatcag
    55561 cataaagctg ttcaccgagc gccagacgtc cggtccctgg cttcagggta aatatcgtct
    55621 cttctttgat aagcaatttg ctgtcggcaa gatggcgaac cttccagacg cccacaccag
    55681 cggcgatacc cagtaccacc agcaataaca agataatcaa taacactttt ttcatgacta
    55741 attcgggcgc tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggtgac
    55801 atcgccacag gcacgtacgg gcatcactgg cattaacgca ttacaaataa ccatctcatc
    55861 tgcctgcaac gcctcctcca gcgaggcttg cacttcgaca agctgataag aggattgtgc
    55921 cagtaaacgg atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg
    55981 cgtataaact acattgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca
    56041 cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcgaat
    56101 caatacttgc tcaagccgat taagatgttt aatacctgca agatgaggat tgcgccccag
    56161 ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc aaacggtcgt aatgtgcagg
    56221 ataagccgta acggagagaa tccgcgttgc tggtccgctg ttcaatgtgc tgtaccctcg
    56281 cccgccacta ccgcgactga tcacgacttt cagcacacca ttttgctgtt ctgctgccag
    56341 cgttttcatc tcttgttcaa gctgaggcca gaagtcacag gaaatcatca accgctgaca
    56401 agcatcctgt agtcgcagga tatgcgccga taacaaactg actttaccgt cgataactct
    56461 ggcggtggta aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc
    56521 ctgcttataa ccgttaatta agaacatacc ggctccttat cagagaattg ttagtgtggc
    56581 agcatgttca ctacggaaca agtcggaata aaagctaaga aaaaaggccc gcaagcggac
    56641 cttttatatg ggtggaaaat gacaacttag atctttttaa agatcaaaga accattagtg
    56701 ccaccgaagc cgaaggagtt acacagcgtg tattccattc cgctgacctg acgtgcttca
    56761 tgcggtacga aatccagatc gcaaccttca tccgggttat ccaggttgat ggttggcgga
    56821 acagcctgat cgcgcagcgc caggatggag tagatagatt ctactgcacc cgccgcacct
    56881 aacaggtgac cggtcataga tttcgtggag cttaccaaca cacggcttgc agcttcaccg
    56941 aagatggttt tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc
    57001 gcgttaacgt agccaatctg actcgcttca atgcctgcat cacgcagagc atttgccatt
    57061 gccagagctg cgcctgcgcc attttctggc ggtgacgtca tatgataagc atcgctgctc
    57121 ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac
    57181 tcttcaagta ccagcatacc ggcaccatcg cccagtacga agccatcacg ctctttatcc
    57241 cacgggcggc tcgccgcttg cgggttatca ttgcgggtag ataatgcacg tgccgcgcca
    57301 aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc cacctgcaac catcacgtca
    57361 gcatcgccat acgcgataat acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag
    57421 gcggtcgcga tagagatgct tgggccacgc aggccataca tgatagtcag atgacctgcc
    57481 accatgttca caatcgttga cggaacgaag aatgggctga tcttacgtgg accaccgttc
    57541 atcagagatg tgtggttttc ttcgatcagt ccgaggccgc caatcccgga gccaattgcg
    57601 gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg catggcctga
    57661 acgccagcga caattccata ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag
    57721 ataatgtcct cacagttaaa atcctttact aagccagcaa atttcgttgc ataggcgcta
    57781 gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg caagcagagc tttccaggta
    57841 gactctacgg tattgccgac aggagacaac atgcccagtc cggtcacaac tacacgacgc
    57901 ttagacacgt ttgtcctcca aggagggaaa aatgattcta gtgggacaaa aagataaaac
    57961 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    58021 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    58081 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cctaggtctt
    58141 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    58201 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    58261 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    58321 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    58381 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    58441 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    58501 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    58561 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    58621 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    58681 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    58741 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    58801 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggtta gtttcgataa
    58861 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    58921 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    58981 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcac
    59041 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    59101 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    59161 ttccttcaaa attcatgatt ttcctctttt aaagctcgag cgccgctgcc atcgctgaag
    59221 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    59281 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    59341 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    59401 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    59461 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    59521 acggtacgct cactggtaac ggcagcgcgc gttttgcgcc agcagcttta caggcagcgc
    59581 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    59641 ttaccggaga aacgacctga ccttctgcag cttcttcaca cgctttcgca ataggcgcat
    59701 catccagacc gatgattgcc gccatagcgc ccgtgccttc cggtacggct tcttgcatga
    59761 acttaccgcg catctcaacc agacgcaccg catcagcgaa atcaatcacg ccagcgcaaa
    59821 ccagcgcgga gtattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    59881 gctgctgcca tacgcgatac agcgcaacag atgcagtcaa cagcgctggc tgggtttgcc
    59941 aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca
    60001 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    60061 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    60121 tttaatcctt atcctagaaa cgaaccagcg cagagcccca agtgaatcca ccgccaaagg
    60181 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    60241 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    60301 ccatcgacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    60361 gaaccagcca gtccagttga gaacggtcaa gattattcgc cgccagcgtc tcatcaacga
    60421 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    60481 ttgaattctc tggattcacg cggtcggcgt ttggcagcgt cagcaattca ccataactac
    60541 cgtcggcatg cagatgggtg gaaatgattc ccggctcttc agaggcagcc agcaccgcag
    60601 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc tgttggatcg caggtgcgcg
    60661 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    60721 cggctacgct taatgcatag gtgaaacctg cacaggctgc tgcaacgtca aatgccgggc
    60781 aacctttaat gtccaacatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    60841 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    60901 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    60961 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    61021 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    61081 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    61141 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    61201 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    61261 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    61321 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    61381 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    61441 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    61501 atcgtcttta acgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    61561 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    61621 accgctgagg catcccgaat actgtcaaga ccctttactt cttcttcacc aatattgagc
    61681 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    61741 gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg cccctaaatc gaggaccacc
    61801 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    61861 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    61921 caggcttgcg ctcgaccttc tttcaccagc tccagggcca tacgcattga actcccacga
    61981 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    62041 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    62101 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    62161 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    62221 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    62281 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    62341 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    62401 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    62461 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    62521 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    62581 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    62641 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    62701 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    62761 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    62821 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    62881 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    62941 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    63001 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    63061 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    63121 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    63181 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    63241 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    63301 ggaaaaactg caaatctctt ttgaatgtgc agcacctgag gtcgacgaaa ccccacgcag
    63361 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc
    63421 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    63481 aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg
    63541 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    63601 aacagaagtg gagccttttg acgtccattt ccgtcaccta agcgaggcgg agattgataa
    63661 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    63721 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    63781 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    63841 tgcgccggat gcggttacgt cgcatccggc aatcggtgca gacggcctga tgcggcgtaa
    63901 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    63961 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    64021 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    64081 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    64141 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    64201 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    64261 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    64321 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    64381 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    64441 ttttcgctac cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    64501 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    64561 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    64621 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    64681 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    64741 gcttaatacg ttttttgttc acccgcactt cacctttacg caaaatacgg taaatcatac
    64801 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cctgcttcgt
    64861 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    64921 attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta
    64981 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    65041 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    65101 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    65161 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    65221 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    65281 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    65341 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    65401 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    65461 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    65521 cgcgttgccc ttgtagatgg gcagcgtctg tatgacctgg atatcgaaag tccagggcac
    65581 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    65641 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    65701 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    65761 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    65821 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    65881 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    65941 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    66001 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    66061 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    66121 gttcgcgcct tccgcgatta tttacgtcag gatatcggcg aaatccttat cgataacccg
    66181 aaagtcctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    66241 aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc actaccagat cgagtcgcag
    66301 atcgagtccg ccttccagcg tgaagtccgt ctgccatccg gtggttccat tgttatcgac
    66361 agcaccgaag cgttaacagc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    66421 atcgaagaaa ctgcgtttaa caccaacctc gaagccgccg atgagattgc tcgtcagctg
    66481 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gccagtacgc
    66541 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    66601 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    66661 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    66721 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    66781 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    66841 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    66901 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    66961 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    67021 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    67081 ccggatgtac cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    67141 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    67201 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    67261 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    67321 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    67381 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcaaactgc cgagacgcgt
    67441 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg
    67501 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    67561 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    67621 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    67681 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    67741 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    67801 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    67861 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    67921 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    67981 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    68041 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactgaggtc
    68101 cctgtcgccg ccgctgtcga acctgttgtt agcgcgccag ttgttgaaga aatggctgaa
    68161 gtcgtagaag cccccgtacc ggttgccgaa ccgcaaccgg aagtggttga aacgacgcat
    68221 cctgaagtga ttgccgccgc ggtaactgaa cagccgcagg tgattaccga gtctgatgtt
    68281 gccgtagccc aggaagtcgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg
    68341 gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt
    68401 gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtggccgcg
    68461 gttgaaccag agatcaccgt tgagcataac cacgctaccg cgccaatgac gcgcgctccg
    68521 gcaccggaat atgttccgga ggcaccgcgt cacagtgact ggcagcgccc tacttttgcc
    68581 ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc atcatgcctc tgccgctcct
    68641 gcgcgtccgc aacctgttga gtaataatta gctcaaagta atcaagccct ggtaactgcc
    68701 agggcttttt tatttcatgt ttgaatcaac atattaaaat tcatcaaatg gcatccttgc
    68761 taaccaacaa tgcaaaatag gcaaaaaaaa tgcccgcccc ggcgatccca gagcgggcag
    68821 acaaaacata cccagtttca tgatatgttt taaagcgagc ttatttgttg agctggaaga
    68881 gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc ctgcaatgcc gtttgctgca
    68941 tgatgtaaga tgaaatagtt gcattccagt ccacatcaac cagatcgctc atctgctgcg
    69001 tttgccctaa agcgcgatcg ctacctaagg aatccagcga ctccagtttg ttcagctgcg
    69061 tgcctaattc cgcgcgaacg gtaaggacat tattcagaga gtttttcagt ccacggttgg
    69121 ttttatccaa tgccgcagcg gcgatttctt tatccgcttc gctatccgcg accggcgttt
    69181 tcagggctgc gatggcgcta tccagcatgg caaaaagatt ggtttcagaa gcgctaccgt
    69241 ctggttccgc taccgcgtta ctggtaatac tgtcgaaaat tttgtcgccc gtatgtccta
    69301 tcaccatcga acgcgaagca tcgacctgtt gcttaatact ttctgttccg ccaacataat
    69361 caccgttagc ctcactaaac ggcgcagtct ctgttttata accggcaaaa atgtagcgcc
    69421 cattaccgtc agtggtgttc gccagattca gcaactggtc acgaagcccc tgaatatccg
    69481 tagccagcga ggcccggtca tcgtcactca aggtgccatt gctggcgtag acaatttttt
    69541 cctgagcatt ctggatagca gtggtgacct ggctaagtac actctcttcc agtgacactt
    69601 tttgagtggc gaaagtacgt gccagcgtgt actggctgtt ttgcgcctgt gcctgggaga
    69661 gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg gttaacgact cgcttacccg
    69721 tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga attggtgatg ccgcgcatgt
    69781 tttgctggta catcatctgg gtactgaaac gcattttatc tccccttagc gaatgttaat
    69841 cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc gcattcgcca ggtaatactg
    69901 ctgaaaacgt tgcagatttc cgtactcctc atcgagattg acaccggaaa tcgactgctg
    69961 ctgattggaa agctgcgtca ccacattacc ttgcgtggtg ctactggttt tcaacgtcgc
    70021 ggttttatta ccgatatcac tcactaacga ggcataagcg tcgttaaagg atttctcacc
    70081 gcccaccgtt ttactgttgc tttgcagatc cagcagggcc tgaccgttgc ggttgtcgct
    70141 atcacccgca tcttcttcgc tcgccatcgc gattttcgct tcgtcggtga ttaatacatc
    70201 catgttgacg atggcgtcac ttactggttt cagcgtgaag ctgtcgttaa cggcaggcgt
    70261 tcctgtaaac gtcaactcca gaccatcaaa tgccacttta ccgttggcat ctggtgtcgc
    70321 cgtaaaagtg gtattgctgg caaggcgggt gacctgccac tgattattat cgaacgagat
    70381 tttgtaatct gtcgccagta ccacggaggc atcagttacc gtggcaccga tcgcaacgtc
    70441 acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg atagtaaaga aatcttcacc
    70501 agcatcgccg ttagcatcaa atccggcttt gtgttggctg ttgaaagcct cggcaaatgc
    70561 cagcgccagt tgtccaagcg tattacgcgt ctggtccaga tcctgagaac ggaatgtcag
    70621 aatgccgccc agcgacccgg tattcagtaa tttctccggg atctcaatat tgcctgccgt
    70681 cccatcaacg taagcgacag tcgtacgaga agggtcggcg ctggaaggaa ctgccgccag
    70741 ttgccgcgcc gtacttccct gaaccagtga gtaaccattg gcatcgtgat gttataagta
    70801 ccgccatcct gaacgctgac ttctacgcca acaatctggt ttaattcgct caccagttga
    70861 tcgcgttgat ccagcagatt gttaggtgac gcccctgccc ccacgcctgt caggcgcgag
    70921 atttgatcgt tcaggctggc aatttgttta gcgtaattgt tgatctgatc aacgctggca
    70981 cctatcgcga tattgacctg tttgtcctgg tcgcgcagat attgatcggt ggttttaaac
    71041 tgattcacca acccttctga tttcccaatc agcgcctggc gcgccgccgg gtcttccgcg
    71101 ttactcacca gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc cagcgaagag
    71161 gtactggtgg agagcatatt gtcgattttc gacatctgct catagcgggc ggtcaggcca
    71221 ctactttgcg tctgcgcagc acgtaactgg ttggtaataa acgtatcata ctcacgctgc
    71281 acaccagaaa cgtagacgcc attgccaacc cagccgccag cgcccaacgt gctattggcc
    71341 tgcgccataa tagtggtttg gcgggtatat ccggcaacgt tatagctgga gatattatta
    71401 cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag
    71461 ctggacatgg aggttcctta taagccttca attacagagt attatcggca gcgacccgcc
    71521 ggacttgagt tattcagaac agattatcaa tgttcatgct gtaggttttg ctcaccttgt
    71581 cgcttatcga tttcatctgc tggatcatgt tggtgagttt acgggcatag tgaggatcgg
    71641 tagcatagcc cgcgtcctgt aggacctgcg ccccctgttc cgcactcgcg gcggtcgtca
    71701 cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta atccgacaat gcttccagat
    71761 acgagctgta gacccgaaac ttcgctttta ctttcttcgc ttcgccgttt tcatattcag
    71821 tcgtggtgat ttcagtgact tgccctttcc agttgccaga ggctttgaca ccaaacaggt
    71881 tatagcttgg ctcgccgttt tcgcggcgga tttgccgttg cccccaacca gattcaagcg
    71941 cagcctgagc gaggatcaaa tgatgtggca caccgctttg ctggcttgcc agttgggcgg
    72001 gcagcgagag ttgcgcgagg aatgctctac tgtcacccgg cagcgaatca tcgtagttac
    72061 gtggtacggc cttttgcacc agctgcgaaa gcgtctggtt ttgataacgc accacggttt
    72121 cgagcgggaa tttcatcggt gctgctggcg tggactcctc tggcaatggt tgttctggcg
    72181 tcatctgttt aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc atctgttggg
    72241 caatttgctg gtcatacata ctggtataca gacgagtgtg ctcgctgctg aacaggccat
    72301 cttttggtaa agcgtcgcgc atgcttttca acatcatctg cacgaacatc ccttccacct
    72361 gacgggccac cggacggata tttgccgccg gatcttcgct cgctttcgcc tttagttcgt
    72421 tgagtgattg cgcatcccag gccgcacttg ccagtagttt gctgtcgctg atcatcagat
    72481 gatttccagt tttgcccgca gacatcccgc actttgcatt gattgcaaaa tagacatcag
    72541 atccatcggc gtagcgccca gcgcattgag cgcacgcacc acgttattga ggctggcgct
    72601 ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt
    72661 aacaacagtc tgtccaccac caaacggtgt atctggctgg ctgacattgg cctgacgatt
    72721 aactgttact gagagatttc cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt
    72781 catcaccacc gaaccggtgc gcgagttaat cactacttta gcgtcctgcg gggtgacatt
    72841 aacctgcata ttctggatat cggcaaggaa acggacctgg gaactgttgc cacttggtac
    72901 gcgcacctga atagtccgcg catctaacgc ggtggcgctg ccatatccac gcacgcggtt
    72961 gatggtgtca gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag
    73021 ggtattcccg acgccaaact ggctgggcaa ttcacgttca ataaccgcac cattggtgat
    73081 ccgtccaccg ttcagttggt taacctgaac actgctaccg cctgcggagg ctcctgcgcc
    73141 gccaaccaga atattgccct gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg
    73201 tgtcatcaac aacgtacctc cacgcaagct tttggcatta cccatggaag aaaccaccac
    73261 atcgatggtt tgcccctgac gtccaaacgg aggaagtgac gctgtcacca ttaccgcagc
    73321 gacgtttttt agctgcatat tggtgcccgt cggaacggta attcccagct gtgagagcat
    73381 gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccag tgccatccag
    73441 ccccactacc aagccatagc caatcagtga gttttgcctt accccctgaa cactggtgag
    73501 atcgcgaata cgctcagcct gagccgccgt cgtaaccagt agaagaatta atgcagagag
    73561 aaatttaatc accacagcct cacttacatt ggcgacaggt taaggaagaa acgctgcaac
    73621 cagcccatat tttgcgcttc gttaatgtag ccattgccta cgtattcaat acgcgcatcc
    73681 gccacctgag tagacggtac ggtattgctg ccgctgatag tgcgtggatt aaccacgccc
    73741 gagaagcgaa taaattcggt accctgatta atggcaatct gtttttcacc caccacatgc
    73801 aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg
    73861 ctggcattgg ccccgccctt tccgttgaac gtgttaccac cggaggcttc gacatcggca
    73921 cgagcgttac caaacagccc ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt
    73981 ttaccgtcac ggctggcatt cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac
    74041 acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag cggttgatag
    74101 ccatagttaa tcggctgagc agactggaaa atagaaccgt tggcgacggg cgtcggaccg
    74161 ggaaccggtt gtgcactggt cgccccctgc accagcggcg tggagggtat ccaggcgcag
    74221 ccggttagtg aaagcaccaa caagctggaa atggcataag tatgcgcagc gtttttttgc
    74281 atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta tgccttagag
    74341 ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc gctttactgt tgatttcgta
    74401 agcgcgttgc acctgaatca tattgaccag ttcttccgcc acgttgacgt tagacgtttc
    74461 aacataccct tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc
    74521 accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagtc cggtatcatt
    74581 cataaaggtg gtgagattga gctgcccaac ctgaaccgga gctgcctggc cttgttgggt
    74641 tacgctgacc acgccatcac gaccgatggt gatacttaac gcattcgccg gaatggtgat
    74701 cgctggctgc acctgaaaac caccagccgt caccagctgc ccgttctgat ccacctggaa
    74761 agagccgtca cggatatagg ctgatgagcc atctggcaac attacctgga aaaagccctg
    74821 ccctttaatc gcgacatctt tgctgttatt ggtctgcgac agattgccct ggctgtgtaa
    74881 gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt aatccggagg gtaaggtggt
    74941 ttgttcggaa gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac
    75001 cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc aggttgttgg caatgacgtc
    75061 catattggtt tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcatata
    75121 atgtcctgta gtggattgct gacaagcaga gcagaagcac gcgacagtgt gaatttcccc
    75181 tcaccctaac cctctcccca aaggggcgag ggaatagtcc gcgcgctttt attgcaatgt
    75241 catcaatcgt tcaattaact catcgacagc agttggttgg cacggcctgc gttatcatcg
    75301 acgctgctga tcaccttcat ctgcatttca aaacgccgcg cgctggcaat catgtcgctc
    75361 attgccgcaa cggcattgac gttactgcct tccagaaccc ccgacatcac acgcaaggtt
    75421 ggatctgcct gcagtaccgg ccctcgcgtg gcctgggttt ctgcgcttaa acgaaaaatg
    75481 ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca ccagtttcag acgccctact
    75541 ggcgcaactg tatttgccgg atcgcccgga ttgagcgccg agattgtgcc atcggcagca
    75601 atcgtgattt ccgccccttc cggcacagca attggccccg cctcgcctat caccggatgc
    75661 ccctgaattg tcagttgccc ggtgggatca acctgaatgc tgccattacg cgtataccct
    75721 tcgctgccgt cagcggtctg cacggccagc catcctgctg caacgcaacg tccagcgggc
    75781 gcgaggtgta atccatcttg ccgggcgtca tgtctgcacc cggtgttgac gccgttacca
    75841 gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc gcgcaaggcg tttaactgcg
    75901 cgcgaaaacc gggcgtagag gcattggcca gattactggc ggttaccgcc tgttgattca
    75961 gcgtctggct ggctgctccc atcgcagtat aaattgcgtg atccattgag ctatcccgtc
    76021 agcgattagc gtaagttaac ccgcgtgttg aggatctggt cctgggtttt gatggtctgg
    76081 gcgttagact tatagttacg ctgggcaacg atcatattga ccagttcttt actgagatcg
    76141 acgttggacg cttccagcgc accgttggtc agggtgccaa agtttcccgt cccggctgtc
    76201 cccaacagcg ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc
    76261 agaccttcgt tgttggcaaa gttcgccagt acaatctgcc ccagcagttg ggtttgttcg
    76321 ttggaattgt tgccgacaac cgtaccgtca tcattgattt gataactcac cagatcgccc
    76381 ggtttgtagc cattctgggt ggttgccaca atgttgttag cgccggtatt ttgctgcatg
    76441 gagttgagga agctcagact aaacgtggcg ggttctgcac cgttaattgc gccggttgcg
    76501 atattattcg ccatcgcacc atccactaat gtgccattag cattaaattc cagtgttgtc
    76561 gctgtcttcg caatgctgtt tggatcactg ctatcctggg tgtagacctg ccagttatta
    76621 tccccggtct tcacaaaata gacgctcatg tcatgagcat taccctgact gtcgaaaaca
    76681 gtcaccgaac cttttttgtt atagctatcc gcattgctgg cgctgaatgg cgtaacagta
    76741 ggaagcggat cactggaatt caggttgatc tgcatcgacg ccgtggtggt agttttcgct
    76801 gccatcaggg tattcgggat cgaaatattg gtcggattcg ccccttgctg aatagtcggc
    76861 ggcgtaccgg ttaccgggta acccgtcagc tgtaaacctt gcgtattcaa caggttacgg
    76921 ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc
    76981 agacggaaaa aaccgttctg gctgatagca acgtccagac ctcgcccggt gttggtggtc
    77041 gtgccatcgg taaagtcctg agtgataccg gcaactttta cccccagtcc cactttcgaa
    77101 ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt ggcggagttg
    77161 gcgatattgt tgccaataac atcgaggttg gtggcggcag cgtttaatcc gctaaccgct
    77221 tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg
    77281 tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgctgtt gccgcggatc
    77341 acaccctgca ccagagcaaa ctgcagcggc tgggcaacca gttgtgtacc accgttactg
    77401 gcgctaatcg ctacattgta agaaccgttc ggcgcagtgc tgccatcagt caacgaaccg
    77461 tcccaggtga aactgtgaac tccggcggtc agttcaccaa tatcaatggt gcgcacaacc
    77521 gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg cagctcaaca
    77581 ccaaacggcg tggtcgtggt cacagcccct tcttcactgc cggttcccgc aagaacagtt
    77641 gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga ctggctgtta
    77701 tcaatctgtc cggaaataga tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg
    77761 ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt cgggtcctgg
    77821 tttttcagct gcgccaccag caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg
    77881 cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc ggtggtggtt
    77941 accgcaatgg acatagcttt ctcctttatt gaccgagcgt aagggttttc agcatcatgc
    78001 ttttcaccgt gttgagcact tcaacattgg cctgatagct gcgtgacgcc gacatggtgt
    78061 taaccatctc cccgacgaca tcaacgttcg gcatttttac gtagcccttt gcatctgcca
    78121 gcggattacc tggttcataa accagtttgt ccggggcctg actttcttaa catcggcaac
    78181 ctttacgccg cctgtcgcag cacctggtgc agcgttaacc tggaatacca cctgttttgc
    78241 ccgatatggc tgtccatcag gaccggtcac gctatcagca tttgccagat tactggccgc
    78301 cacgttcagg cgctgggact gggcagttaa cgccgacccg gcgatatcaa aaatattcag
    78361 cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga tttgcccgct
    78421 caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg
    78481 atccatatcg acggtattac cgtcaaacga aggctggtcc ggaatacggt attgcagttc
    78541 tgcggtagga ggcgtcagcg cctgcgccgg gatgtgttgc gttgaggtca tcgtcagtgc
    78601 aaccacactg gttgcatccc gtccacgttg catgactttt ttaagttcac tggcaaaatc
    78661 gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg ccagcacttc
    78721 ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt
    78781 atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat gcctttacgc
    78841 gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg atggtcattg
    78901 cggacaggta caattcacgt tgtagaaatg gctgggggcg aaaatgctga caataaaacg
    78961 tagcgtggcg atcatcgcga tactgttcag tccgttaagt acggcgagca atctcacatc
    79021 gcaattgcac aactttttta gcgcccaact cgcgggggta agtgatgagg ttcgtgtttc
    79081 tattcgtaca gcgcccaatc tactaccgcc atgcgagcag ccattgcttt cgatgagcaa
    79141 taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg acaaacgata
    79201 tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt gccgcgatgc ctattgtgcg
    79261 gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt ggacgcctgg ataccctgcc
    79321 accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc gcgatctatc
    79381 acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa aagcgggaca
    79441 acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc aacgcagaag gtcaggcgct
    79501 gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg gtatcgggac aggtagtcag
    79561 cggcgttgtt gatgcagatg ggaatattct tataaacctg taagctgtta aagattaccc
    79621 gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg aataaaccat
    79681 gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc gttcaaccgc gcgaaaccac
    79741 cgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc gcctccacca gcaccagtgt
    79801 gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata tcaatcttga
    79861 acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg acaccggcaa
    79921 aattgccgat gcgctgatca acgaagcgca gcaagacttg cagagtaact gatcgtatga
    79981 cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc aaaacggtaa
    80041 tggatcaaga gcagcaacat ctctctatgg ggcagatcaa cggcagccag ttgcaatgga
    80101 ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag ttacgcaaga
    80161 aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa attactgtga
    80221 aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa ggacagattg
    80281 agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg ctatatgggg
    80341 cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa gatttcgatc tgaatattat
    80401 ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc aaattcttta
    80461 actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc cagcacgacc
    80521 gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg catgatatgt
    80581 aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg caacagaaac
    80641 gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca gtaaagcagc
    80701 gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccggcatg tttcaacgga
    80761 ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc aattttcact
    80821 ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca acactttcac tacaatcagg
    80881 ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc atcaaacgcg
    80941 gtaaatttac cgtactggaa cagcgaaacg gttaacggac ctgaaagaat gcccaacgca
    81001 accgcactcg gcagtgccaa caggaaacaa agacgcaacc cccagtccat caaacggttg
    81061 tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa aatggtgcca
    81121 agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta atacatccaa
    81181 gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa ggagatctgg
    81241 ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat tgctccagca
    81301 tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg cggtagctga
    81361 tacaccagct gcaggatgcc gccgaccgta acagcccacg ccagcgccag caccggtggg
    81421 ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag cagtgttggc
    81481 gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac cagcgacgcc
    81541 agggagatca gcaagatata gggaaaggta atctttagta gctggctggt cagggcaaat
    81601 ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc
    81661 atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac ataagagaca
    81721 aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat cggtacaaat
    81781 gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag tttaaaagcg
    81841 acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat tgcgtctcgt
    81901 gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag cgattttaat
    81961 aaattcatcg gtgttctaat ccggacccac gggcaaaacg cctgcaaggc aggcgctgaa
    82021 agaggcgaat agtctaccta gattatggtg aattgctacc gccagatgtt acagggttta
    82081 ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca ctgcctattc
    82141 gccggcggtt tgcggaactg tctgattttg cacgcattca atgaagtgcc gcgcacagcc
    82201 gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat tcactacacc
    82261 ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg caccgtcagt
    82321 cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacaag tggtgatctg
    82381 caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag
    82441 tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg tatccaccac
    82501 atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacgc tattgctacg
    82561 atgtttatcc attcttagcg aggctgcggt ggcgagttgc gtttttaact caccgtagag
    82621 tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg ccgccagttc
    82681 caccagcctt tcagcatcgc gcagattttc tgccagcggt ttatcgacac agacatgtac
    82741 ccccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg agtgcacaaa
    82801 aaccgcatca caactggcgg caaggctgga taacgaatcg gcataaggaa tgcgccagct
    82861 gtcacaaatt ggcagggctt tggcgcgcgt aggcgaccag gctccttgta acgtccagtc
    82921 agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta atcccactac
    82981 gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa taaagacgtt
    83041 gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggtctgtaaa tcaccgtcaa
    83101 ccatgttcga catagccgtt accgccggct gatcttcaac ctcaccactg aaaagatgca
    83161 tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa ggaccatctt
    83221 cgcgattcgc cagttgttcc agcgtcgact ccacttccgc catatcgctg aattcataca
    83281 ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc accacgcaat aacaacgtgg
    83341 tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac
    83401 gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga taatgacgtt
    83461 tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg accggttcac
    83521 ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg tattgctccg
    83581 gcgtggtcac ctgcttttcc agcaagcaac caattacgcg agcttccagg gcagtaagtt
    83641 gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt taatgccgtc
    83701 agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata gccttctttt
    83761 tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat ataattagcc
    83821 ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt cagggcttca
    83881 aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata gcaggcatga
    83941 aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg
    84001 tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat catccctagc
    84061 ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc ccagggcttg
    84121 aggaaatggc gattctctgc gtaataatcc gccagacgcc aggcatcacg atcatgcacc
    84181 agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact gcgatagcca
    84241 aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta ttactatacc
    84301 tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt cacatatttg
    84361 ataaaaactc tctatcaaag cgacgttttg ataaaaaaat attgtccccg aagtgatggg
    84421 aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc gggaggggaa
    84481 atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat cgataatatg
    84541 ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa
    84601 atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt tattcagcaa
    84661 ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt
    84721 accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca cgaaccgtgg
    84781 ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga tccgccgcgt
    84841 tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt ctcgctgttg
    84901 atgatgcagg acagtgccag tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa
    84961 tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctatg tgcggcgttc
    85021 aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt tcgcgaaggc
    85081 atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct ggcgggttac
    85141 tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga cgtggctggc
    85201 gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc
    85261 taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt gatggctggg
    85321 ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct acaacaactt
    85381 ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc
    85441 ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt tagccgtctg
    85501 ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt tgacctgggc
    85561 aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg catcttcact
    85621 ttccttgcgc tgggttggca gtttagtcag aaacgcgccg cgcgtcgttt gctggaacgc
    85681 gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact gacagccaaa
    85741 tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct caaagcccgc
    85801 atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa cgacgacgca
    85861 gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa agatgacagc
    85921 cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga cggcaaaccg
    85981 ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt caatggctac
    86041 gccaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt ttctactccc
    86101 gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt tgccgacctg
    86161 ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc gctggacaaa
    86221 ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat tcagctattt
    86281 ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg cgttgctaat
    86341 taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat ggcgatttaa
    86401 tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt tttttctgct
    86461 ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg cagatttcat
    86521 gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca gtggcctgct
    86581 cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta accagtcgct
    86641 tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg acgcccatat
    86701 tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg ttaccctaac
    86761 cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga ccatgaaact
    86821 gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat
    86881 ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct
    86941 tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt acgtcctgcg
    87001 cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa
    87061 gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc gcgcccttta
    87121 tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa acatcccttc
    87181 agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc gccgcccaga
    87241 cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc cgtataccag
    87301 cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga ccacagttga
    87361 ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc acgatttcac
    87421 cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg agcgcggatt
    87481 taatgaagcc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca ctaactccag
    87541 ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg
    87601 tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt ttgatcgtga
    87661 agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg cgctgaaagt
    87721 cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg
    87781 gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata tgctggttgg
    87841 tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc accaacaggc
    87901 attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg attctgcgcc
    87961 acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca acgccccaac
    88021 cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc actttgaagc
    88081 attctgttct gtaaacggcc cgcagttcta tggcttgccg gtcaacgaca cattcatcga
    88141 actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg acaccctggt
    88201 gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat cgtagcttcc
    88261 tgttgtcatt aggttatttt atctgtataa ataaccagta tattcaacag ggggctatta
    88321 tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct attgacgccc
    88381 tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa ggccacgtat
    88441 cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa gaagataaac
    88501 agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg
    88561 aataatatgc agtgattttt gtttttgccg gatcgccccg gcttttttat tttacttacg
    88621 aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg ctaaaaaaat
    88681 atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt tatctgttcc
    88741 cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg cttcagataa
    88801 aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc catgatggaa
    88861 aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag caccgttgat
    88921 agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc cgagcaggaa
    88981 gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca atttatttcg
    89041 atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa cgagtatcgg
    89101 cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc tttaccattt
    89161 ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag cagagagaaa
    89221 aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc cgccgggtat
    89281 tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc accgcatcaa
    89341 cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga aggcgaaaag
    89401 tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg aactttcccg ccacaacgaa
    89461 gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga ctccgcattt
    89521 ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa gctcgatgcg
    89581 caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat cggcttattt
    89641 gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat ccaactggcg
    89701 aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg tcatgacgat
    89761 gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc gattgtctgc
    89821 gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt acgtaaagta
    89881 tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc ggcgtttacc
    89941 ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga cgcgttgaag
    90001 attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt tccgtttgcg
    90061 gaagtggcca gtgatgggtc agaagccttc ccgttcttgc gcaatgtatt gccgggtatc
    90121 ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga agattttatt
    90181 atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag tgggcacggt
    90241 tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga ccaaaaaagc
    90301 gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt acggcctcca
    90361 gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat ctcatcaata
    90421 atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca attatggatc
    90481 tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa tatttaatct
    90541 ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag cactcaccag
    90601 acatctctga tgctgaaccc actaaactta tgaagattct ttcattatta attcaaaatt
    90661 attgaaataa aaataaatat cattcaattt gttatttttg tgttgcaatt cattctccga
    90721 cacgccattt tctttctctc gcaaattcgt attggatgca cttatgtcat tcacaaatac
    90781 ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa ttattgtcta
    90841 cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg gctggtatca
    90901 caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc tggttattcg
    90961 cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt cgcccatgac
    91021 tcgtcttgcc gcaaaagctg gacatctcgc attgtaccta ttgctgttcg ccatcggcat
    91081 cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg gctggtttga
    91141 cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg ccctgcattt
    91201 ttggctcgcg tggagcgtcg tagtactgtc tgttatgcac ggatttatgg ccctgaagca
    91261 tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt catctgacta
    91321 tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc
    91381 tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc acgcctttgt
    91441 taacttccgc atacagcatc ttggctatag ctggttatat ggcaccttta aagatttcga
    91501 cggtactttt acctttgatg aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa
    91561 caccaccagc gtcgatacta accacgccga acgcgataaa catcttcgca gtgcggattt
    91621 tctcaatacc acaaaatatc cacaggcaac attcacctcc accagcgtga agaaagacgg
    91681 tgacgaactg gatattaccg gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct
    91741 ggaagcgaaa ttaattggtc agggcgacga cccatggggc ggtaaacgtg caggctttga
    91801 ggccgaaggc aaaattaagc tcaaagactt caatatcaag acagatttag gtccagcttc
    91861 tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag agtgaaatgt
    91921 tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa tacccagtgt
    91981 tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac gtcctgcccg
    92041 acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaatct cactacaaca
    92101 acctttgtat ttttccgcgc atactgggca ctgaataaac agcaggtggc agccatcatt
    92161 tttacagttg gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg cgataatctc
    92221 atccgatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac
    92281 cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct cgatatgcca
    92341 gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac gaatgccgcc
    92401 ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga ctgcttttgg
    92461 cagctgctca cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc
    92521 atactcatag tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt
    92581 cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag gatcgtcaat
    92641 accgtcggca acaatgcgat cgcgtacctt catgcgcagt acccagaagg atttcccgtc
    92701 atcatccaac gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg
    92761 cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg
    92821 cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata aagcgtcacg
    92881 ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc
    92941 gctctcagcc aacattttgg cttttaacgc gtcgttggaa atgcggttgt gtaacactgg
    93001 catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat atagcaatta
    93061 tcgataatta acatccacac attttacgct acatttgcgc attaaaaatt atttgttatt
    93121 tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat
    93181 gcgacaatac atacaattgc ccgtataggt tgaaaaacag gattgatatg acgaatctac
    93241 ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg ggtattggcg
    93301 tactttggtt ggtcgtgcaa ttgccctacc cgattatcta ccgcctcggt tgtggattag
    93361 gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac
    93421 tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat
    93481 ccgttggcat gggcctgatg gaaaccggta tggcgtggtt ctggccggac cgccgaatcg
    93541 cccgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg
    93601 gcatcctgtt agttggcatc cattttctga cactggagct gggcgcgcgg cagtttggta
    93661 tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt gactggctac
    93721 aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca
    93781 tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accagatcat gattacggcc
    93841 cgcgctcaag cgttttcgtc ccgttatttg ccgttgagca ggctgcgacc acgaccggaa
    93901 cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc
    93961 cagatggcaa agggtatcaa ttgattatgc tgccaccaga gtgttctccg ccactggatg
    94021 atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc atgatggcac
    94081 cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc gttccttcac
    94141 gctattaaat ctcccatgcc ggatgcttca gaatggcatc cggcattacc acagcaaatc
    94201 cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg gcgcataatt
    94261 agtgtgttta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag cggattgcta
    94321 tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc gtcgcctggc
    94381 taggctgttt tcttaccggg gccgccttca gtctggtaat gcccttctta cccctctacg
    94441 ttgagcagct tggtgttacc ggccactccg ccctgaatat gtggtccggt attgtcttca
    94501 gtataatggg ttcctaaaca catcatttta ggtattgaat atgagacggg caatcagcta
    94561 catcagattt tcaagtgaac gacaactgaa aggggactct gtacgccgac aatctaagct
    94621 ggttactgac tggcttgata agaaccctga gttttacttg gattcatcct tgagctttaa
    94681 agatttgggg aaaagtgctt ttagtggtaa gcacctgaaa ggtggtctag gtgacttttt
    94741 aaccgctatc gagaaagggc ttgttaaagc tggtgatacg ctactaatcg agagtttaga
    94801 ccgtctgtca cgtcaggata ttgatattgc ttctgagcta ttacgccgta tcttaagggc
    94861 tggtgtagat gtagtcacac tgagcgatgg agaacattac acacgagaaa gtttaaaaga
    94921 cccgttagcc ctgattaaaa gcattctgat aatgcagcgg gcgcatgagg aaagtttgag
    94981 aaagtctgag cgtgtccaag ctgcatggaa ccgtaaaaaa gaactcatct ccgaaggtat
    95041 caaggtatct aggcgttgtc ctgcatggtt aaggctgaat gatgaccgtc gaacattcac
    95101 aatcattcct gataaagtgg aagttgttaa acgagcattt gacctgagat tacagggttt
    95161 aagcttttgg gctatcacca gaactttaa
//
LOCUS       DAJTRB010000003        72916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTRB010000003 DAJTRB010000000
VERSION     DAJTRB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.003"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO330_000238"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO330_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5881256.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO330_000239"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO330_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5881257.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO330_000240"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO330_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5881258.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO330_000241"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO330_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5881259.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO330_000242"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO330_000242"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5881260.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO330_000243"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO330_000243"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5881261.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO330_000244"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO330_000244"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5881262.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO330_000245"
     CDS             complement(4598..5320)
                     /locus_tag="OO330_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5881263.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMAGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6317)
                     /gene="fkpA"
                     /locus_tag="OO330_000246"
     CDS             complement(5487..6317)
                     /gene="fkpA"
                     /locus_tag="OO330_000246"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5881264.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPKADAKPEADAKAADSAKK
                     "
     gene            6538..6756
                     /locus_tag="OO330_000247"
     CDS             6538..6756
                     /locus_tag="OO330_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5881265.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6805..7395)
                     /gene="slyD"
                     /locus_tag="OO330_000248"
     CDS             complement(6805..7395)
                     /gene="slyD"
                     /locus_tag="OO330_000248"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5881266.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7490..7690)
                     /locus_tag="OO330_000249"
     CDS             complement(7490..7690)
                     /locus_tag="OO330_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5881267.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7700..9437)
                     /gene="kefB"
                     /locus_tag="OO330_000250"
                     /pseudo
     CDS             complement(7700..9437)
                     /gene="kefB"
                     /locus_tag="OO330_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9437..9988)
                     /gene="kefG"
                     /locus_tag="OO330_000251"
     CDS             complement(9437..9988)
                     /gene="kefG"
                     /locus_tag="OO330_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5881268.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10119..12032
                     /locus_tag="OO330_000252"
     CDS             10119..12032
                     /locus_tag="OO330_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5881269.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12032..13054
                     /locus_tag="OO330_000253"
     CDS             12032..13054
                     /locus_tag="OO330_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5881270.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLANEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13048..13266
                     /locus_tag="OO330_000254"
     CDS             13048..13266
                     /locus_tag="OO330_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5881271.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13320..14189
                     /locus_tag="OO330_000255"
     CDS             13320..14189
                     /locus_tag="OO330_000255"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5881272.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSRETVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14244..14648)
                     /locus_tag="OO330_000256"
     CDS             complement(14244..14648)
                     /locus_tag="OO330_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5881273.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14950..15582
                     /gene="crp"
                     /locus_tag="OO330_000257"
     CDS             14950..15582
                     /gene="crp"
                     /locus_tag="OO330_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5881274.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15633..17723
                     /locus_tag="OO330_000258"
     CDS             15633..17723
                     /locus_tag="OO330_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5881275.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLRLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17790..19010)
                     /gene="argD/dapC"
                     /locus_tag="OO330_000259"
     CDS             complement(17790..19010)
                     /gene="argD/dapC"
                     /locus_tag="OO330_000259"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5881276.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19096..19659)
                     /gene="pabA"
                     /locus_tag="OO330_000260"
     CDS             complement(19096..19659)
                     /gene="pabA"
                     /locus_tag="OO330_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5881277.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19691..20293)
                     /locus_tag="OO330_000261"
     CDS             complement(19691..20293)
                     /locus_tag="OO330_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5881278.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20283..20450)
                     /locus_tag="OO330_000262"
     CDS             complement(20283..20450)
                     /locus_tag="OO330_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5881279.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20555..21136)
                     /gene="ppiA"
                     /locus_tag="OO330_000263"
                     /gene_synonym="rotA"
     CDS             complement(20555..21136)
                     /gene="ppiA"
                     /locus_tag="OO330_000263"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5881280.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21407..22588
                     /gene="tsgA"
                     /locus_tag="OO330_000264"
     CDS             21407..22588
                     /gene="tsgA"
                     /locus_tag="OO330_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5881281.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22850..25393
                     /gene="nirB"
                     /locus_tag="OO330_000265"
     CDS             22850..25393
                     /gene="nirB"
                     /locus_tag="OO330_000265"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5881282.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25390..25716
                     /gene="nirD"
                     /locus_tag="OO330_000266"
     CDS             25390..25716
                     /gene="nirD"
                     /locus_tag="OO330_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5881283.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25842..26648
                     /gene="nirC"
                     /locus_tag="OO330_000267"
     CDS             25842..26648
                     /gene="nirC"
                     /locus_tag="OO330_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5881284.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26667..28040
                     /gene="cobA"
                     /locus_tag="OO330_000268"
     CDS             26667..28040
                     /gene="cobA"
                     /locus_tag="OO330_000268"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5881285.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28287..28454
                     /locus_tag="OO330_000269"
     CDS             28287..28454
                     /locus_tag="OO330_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5881286.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28749..30086
                     /gene="frlA"
                     /locus_tag="OO330_000270"
                     /pseudo
     CDS             28749..30086
                     /gene="frlA"
                     /locus_tag="OO330_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30107..31129
                     /gene="frlB"
                     /locus_tag="OO330_000271"
     CDS             30107..31129
                     /gene="frlB"
                     /locus_tag="OO330_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5881287.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31179..32009
                     /gene="frlC"
                     /locus_tag="OO330_000272"
     CDS             31179..32009
                     /gene="frlC"
                     /locus_tag="OO330_000272"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5881288.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32006..32791
                     /gene="frlD"
                     /locus_tag="OO330_000273"
     CDS             32006..32791
                     /gene="frlD"
                     /locus_tag="OO330_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5881289.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32891..33622
                     /gene="phoB"
                     /locus_tag="OO330_000274"
     CDS             32891..33622
                     /gene="phoB"
                     /locus_tag="OO330_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5881290.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33782..34867)
                     /locus_tag="OO330_000275"
     CDS             complement(33782..34867)
                     /locus_tag="OO330_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5881291.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34879..36183)
                     /locus_tag="OO330_000276"
     CDS             complement(34879..36183)
                     /locus_tag="OO330_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5881292.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36195..36548)
                     /locus_tag="OO330_000277"
     CDS             complement(36195..36548)
                     /locus_tag="OO330_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5881293.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36559..37437)
                     /locus_tag="OO330_000278"
     CDS             complement(36559..37437)
                     /locus_tag="OO330_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5881294.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37434..38660)
                     /locus_tag="OO330_000279"
     CDS             complement(37434..38660)
                     /locus_tag="OO330_000279"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5881295.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38661..39824)
                     /locus_tag="OO330_000280"
     CDS             complement(38661..39824)
                     /locus_tag="OO330_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5881296.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39908..40270)
                     /locus_tag="OO330_000281"
     CDS             complement(39908..40270)
                     /locus_tag="OO330_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5881297.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40287..41192)
                     /locus_tag="OO330_000282"
     CDS             complement(40287..41192)
                     /locus_tag="OO330_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881298.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41462..42466)
                     /gene="trpS"
                     /locus_tag="OO330_000283"
     CDS             complement(41462..42466)
                     /gene="trpS"
                     /locus_tag="OO330_000283"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5881299.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42459..43217)
                     /gene="gph"
                     /locus_tag="OO330_000284"
     CDS             complement(42459..43217)
                     /gene="gph"
                     /locus_tag="OO330_000284"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5881300.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43210..43887)
                     /gene="rpe"
                     /locus_tag="OO330_000285"
     CDS             complement(43210..43887)
                     /gene="rpe"
                     /locus_tag="OO330_000285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5881301.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43905..44741)
                     /gene="dam"
                     /locus_tag="OO330_000286"
     CDS             complement(43905..44741)
                     /gene="dam"
                     /locus_tag="OO330_000286"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5881302.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44848..46134)
                     /gene="damX"
                     /locus_tag="OO330_000287"
     CDS             complement(44848..46134)
                     /gene="damX"
                     /locus_tag="OO330_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5881303.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46226..47314)
                     /gene="aroB"
                     /locus_tag="OO330_000288"
     CDS             complement(46226..47314)
                     /gene="aroB"
                     /locus_tag="OO330_000288"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5881304.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47371..47892)
                     /gene="aroK"
                     /locus_tag="OO330_000289"
     CDS             complement(47371..47892)
                     /gene="aroK"
                     /locus_tag="OO330_000289"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5881305.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48293..49531)
                     /gene="hofQ"
                     /locus_tag="OO330_000290"
     CDS             complement(48293..49531)
                     /gene="hofQ"
                     /locus_tag="OO330_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5881306.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49443..49847)
                     /gene="hofP"
                     /locus_tag="OO330_000291"
     CDS             complement(49443..49847)
                     /gene="hofP"
                     /locus_tag="OO330_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5881307.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49837..50277)
                     /gene="hofO"
                     /locus_tag="OO330_000292"
     CDS             complement(49837..50277)
                     /gene="hofO"
                     /locus_tag="OO330_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5881308.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50261..50800)
                     /gene="hofN"
                     /locus_tag="OO330_000293"
     CDS             complement(50261..50800)
                     /gene="hofN"
                     /locus_tag="OO330_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5881309.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50800..51579)
                     /gene="hofM"
                     /locus_tag="OO330_000294"
     CDS             complement(50800..51579)
                     /gene="hofM"
                     /locus_tag="OO330_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5881310.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51699..54251
                     /gene="mrcA"
                     /locus_tag="OO330_000295"
     CDS             51699..54251
                     /gene="mrcA"
                     /locus_tag="OO330_000295"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5881311.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54416..54976)
                     /gene="nudE"
                     /locus_tag="OO330_000296"
     CDS             complement(54416..54976)
                     /gene="nudE"
                     /locus_tag="OO330_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5881312.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55296..57431
                     /gene="igaA"
                     /locus_tag="OO330_000297"
     CDS             55296..57431
                     /gene="igaA"
                     /locus_tag="OO330_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5881313.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57496..58164
                     /gene="yrfG"
                     /locus_tag="OO330_000298"
     CDS             57496..58164
                     /gene="yrfG"
                     /locus_tag="OO330_000298"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5881314.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58175..58576
                     /gene="hslR"
                     /locus_tag="OO330_000299"
     CDS             58175..58576
                     /gene="hslR"
                     /locus_tag="OO330_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5881315.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58601..59470
                     /gene="hslO"
                     /locus_tag="OO330_000300"
     CDS             58601..59470
                     /gene="hslO"
                     /locus_tag="OO330_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5881316.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRECCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59614..61338)
                     /locus_tag="OO330_000301"
     CDS             complement(59614..61338)
                     /locus_tag="OO330_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5881317.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61717..63339
                     /gene="pckA"
                     /locus_tag="OO330_000302"
     CDS             61717..63339
                     /gene="pckA"
                     /locus_tag="OO330_000302"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5881318.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63415..64767)
                     /gene="envZ"
                     /locus_tag="OO330_000303"
     CDS             complement(63415..64767)
                     /gene="envZ"
                     /locus_tag="OO330_000303"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5881319.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64764..65483)
                     /gene="ompR"
                     /locus_tag="OO330_000304"
     CDS             complement(64764..65483)
                     /gene="ompR"
                     /locus_tag="OO330_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5881320.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65711..66187
                     /gene="greB"
                     /locus_tag="OO330_000305"
     CDS             65711..66187
                     /gene="greB"
                     /locus_tag="OO330_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5881321.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66284..68605
                     /locus_tag="OO330_000306"
     CDS             66284..68605
                     /locus_tag="OO330_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5881322.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69043..69270
                     /gene="feoA"
                     /locus_tag="OO330_000307"
     CDS             69043..69270
                     /gene="feoA"
                     /locus_tag="OO330_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5881323.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69287..71608
                     /gene="feoB"
                     /locus_tag="OO330_000308"
     CDS             69287..71608
                     /gene="feoB"
                     /locus_tag="OO330_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5881324.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71608..71844
                     /gene="feoC"
                     /locus_tag="OO330_000309"
     CDS             71608..71844
                     /gene="feoC"
                     /locus_tag="OO330_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5881325.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72047..>72916
                     /gene="rpnA"
                     /locus_tag="OO330_000310"
     CDS             72047..>72916
                     /gene="rpnA"
                     /locus_tag="OO330_000310"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5881326.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
BASE COUNT        17566 a        19761 c        18445 g        17144 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca aaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccaaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggatta tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgccatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcttt gcatcagcct tcggcgctgg tttcacatcc
     5581 agcagctcta cgtcaaacac tagggtagaa ttcggtggga tccccggaac acccgctttg
     5641 ccgtaagcca gttctggtgg aataaccagt ttgatcttac cgcctttctt gatgttcttc
     5701 agaccttctg tccaacccgg gataacaccg tccagacgga aagaaagcgg ttcaccacgg
     5761 gtgtaagagt tgtcgaactc tttaccgtcg atcagcgtac ctttgtagtt cactacaaca
     5821 gtatcgctgt ctttcggtgc ttcgccttta ccggcttcta ctacctgata aaccagacca
     5881 gttgaagagg ttttcacacc tttctctttg gcaaatttct cgcggtactc tttacctttt
     5941 gcttcgttat cagccgcgtc tttttccatc ttcgcctgag cagaagactt cacgcgagct
     6001 tcgaatgctt gcagagtctg ttcgatctct tggtcggaga gtttgctctt atcagcaaat
     6061 gcatcctgaa caccagcgat cagctgatct ttatccagtt tgatgcccag tttttcttgt
     6121 tctttaagag agttttccat gtaacgaccc agcgaagcac ccagtgcata agctgatttc
     6181 tgatcgtcat ttttgaacga tgctttgctg tctgcagttg tagcaggttt tgcagcttca
     6241 gcagcaaaag tgattggtgc atgcagggca acggccattg tggtcgccag tagcgttact
     6301 ttaaacagtg atttcatcca tatctccagg atcggggcat ctcaccccag ggttaactat
     6361 tatcagaagg gtactataaa gcgttgtcga acaaatctac atacagacac gccctattat
     6421 catctatttt cagactcttt ttgtttaaat tagtttcgat gaccgcgaaa tgagtgctgt
     6481 ctcgggcagc aaagttaagt agaatccgcg gcgaccattc gacaaaagag gtgaatcatg
     6541 caggatttat cattggaagc acgcctggca gagctggaga gccgactggc ttttcaggaa
     6601 atcaccattg aagaactgaa cgtcacggtg accgctcatg aaatggagat ggcgaaactg
     6661 cgcgatcatc tgcgtctgct gaccgagaag ttaaaagcca gccagccgtc gaacatcgcg
     6721 tcgcaggctg aagaaacgcc accgccacat tattgaggcg taaaaaaagc ggggattccc
     6781 cgcttttttg tcaccttttc ggtattagtg gcaaccacaa ccgccgttgc ctttaccgcc
     6841 acagcagcct tcgccaccgt gttcatgacc gtgatcgtgg ccatgaccgc cgcagcaacc
     6901 gtcgtggtcg tgatcgtggt gatgatcgtg cgcgccgtga acgtggccat gagccagttc
     6961 ttcttcggtc gcttcgcgaa tcgccacaac ttcaacgttg aatttcaggt tctgaccggc
     7021 cagcatgtgg ttaccatcaa ccactacgtg atcgtcttca accgcagtga tttcaaccgg
     7081 taccggaccc tggtcagttt cagccaggaa acgcatacct acctgcagtt catcaacgcc
     7141 cataaatacg tctttaggaa cacgttgcac taggttttcg tcgtactgac cgtaagcgtc
     7201 gttcgcgcca acagcgacat caaatttgtc gccaacttca tgaccttcca gcgccgtttc
     7261 caggccagag atcagggaac cgtgaccatg caggtagtcc agcggcgcac tcaccggaga
     7321 ctcatcaacc aacacaccgt cttctgtacg tacctgatag gccaggctga ccaccaggtc
     7381 ttttgctact ttcatgatat ctcctgagca tgggaagaat agtggcgcag attgtagcgg
     7441 aattctgcag ccgtgtactc actagcttaa aaaaactcgg cgcatatcgc tagtccggat
     7501 gaaaaatccc gatcacttgc tcatctttgt gaacgtgatc gcgggcttct ttgtctgctt
     7561 ctcgcatctg atgtccgcac ttaacacatt caacaatatc aatattattt tcgcgccaca
     7621 tcgccattga atcctgcgcc tgacaggccg ggcattttgc gcccgctata aaacgttttc
     7681 ggattgccat ccttaccctc tactcaaatt catcccagcc gtccagctgg cgtcgttctt
     7741 gttgcatttc acgctggaaa atctcttcca gttcgcgtcg ggcttccctg gcgcgagaaa
     7801 tttgtacggt atcagcatgc atcgggatta actcccgcag cattcgcata tccaggcggc
     7861 gaaaatgcag ttgcgcccgc tgtgcctgat gcggatgcat gccaagcgtg accagcgtct
     7921 tgcgccccag ctctaacgca ctggagaatg tttcacggga aaactgcgtc acccctgcct
     7981 gtaataactc atgcgcttcc acacgtccgc gcgctcgcgc aagaatatgc aaatgcggaa
     8041 agtgctgctg gcatatttcc accagcttca tggtgtcttc cggctcgtta caggtaatga
     8101 cgatagactc agcggcctct gcacccgcag aacgtaaaag atcgacctgc gtggcgtcgc
     8161 cgtaataaac tttgtagcca tatttacgca tcaggttaac ggcgctgata tcccgctcca
     8221 gcacggtaat gcgcatttta tttgccatca gcaaacgacc aatcacctga ccaaaacgcc
     8281 cgaagcccac gacaatgacc tggggtttat catcgttgac ccacggtttt tcgtcttctt
     8341 cttccggtcc gttaaactgg cgggatagcc atttatccac cagcttcatc agcaacggcg
     8401 ccatctggtc gccctggaat aaacgttgtg aagaagcggt agaaaagagg acaaaggcaa
     8461 actcaccgcc ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat
     8521 acaatcgcgc cagcagatac agcacgagaa ttttcaccgc caccagcaca accacgctta
     8581 tcactaccca caacagatgg gtataaagca ccccgaggtt gagcgacatg ccgacagaga
     8641 taaagaacaa accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc
     8701 gatattcact ttccgccagc agcacgcccg caataaatgt accgagcgcc atcgacagcc
     8761 ccagcgcatc cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca
     8821 cttcccgcac gccagaagct gcaataaagc ggaataccgg acgcagtaaa tagcgcccac
     8881 caatcagcat gccgacaaac tccagcacct tcatgccgat cttcatccag tcgaaatgtt
     8941 cgtctgccga ccccgccaac aacggcacta acgccagtgc tgggatcact gccagatcct
     9001 gaaacagcag aaccgaaaac ccgagctggc cggattcgct gcgattcatc cctttctcac
     9061 gcatcaattg caacgccatt gcagttgaag acatcgcaaa gccaatgcca ccgaccaccg
     9121 ccgcctgcca ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca
     9181 gcacctgtgc cgcgcctacg ccaaaaatcg aacgccgcag ttgccaaagt ttggagggat
     9241 tcaactcaag gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga
     9301 tctcatcgac gtcgctaata aaccccagcc cccacgggcc aattgcaatc cccgccagca
     9361 aatatcccaa cacagcgcca atccccagcc gcgaagagaa acagcactcc tgcgagtaaa
     9421 aaatcggaac cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt
     9481 aggctctggc gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga
     9541 tgggacttaa ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat
     9601 cgctcatcgg gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg
     9661 taatcacgtt acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac
     9721 cacgacttaa tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa
     9781 gaggatgctg aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat
     9841 caataaaaaa atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg
     9901 tggccggttt aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca
     9961 gcagcaaaac tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag
    10021 tcgctgtttt gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca
    10081 taatgataag ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac
    10141 aaattcgtcg cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc
    10201 agaaagtcgg cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga
    10261 aaaatgaaat cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt
    10321 gggtgaatca ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg
    10381 accgtgaata tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc
    10441 acgccattgc gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc
    10501 gtgccgccag cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa
    10561 gtgatttctc cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt
    10621 cagacttgct gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc
    10681 tggaaaaatg gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact
    10741 tcctcgatcc gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca
    10801 ccggcaacta cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga
    10861 tgtacgaaag ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg
    10921 ccaaagccac caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc
    10981 tgattgcccc cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc
    11041 tgccaaatcc gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc
    11101 tcgactcgat taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg
    11161 gcgcaggtaa atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg
    11221 aaattggtct ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc
    11281 tgcgcgccga cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac
    11341 aaaaactgcg tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa
    11401 cgcgccgctt ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc
    11461 gtccgaatct gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg
    11521 cactcaccga agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc
    11581 atttgctgcg ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt
    11641 tcgacggcga tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc
    11701 agaccgacga agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc
    11761 gtcgggaagc ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg
    11821 aaaaagagat ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca
    11881 gcgaactgta tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca
    11941 gcgccaaatc cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg
    12001 agcagatgct gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa
    12061 tgaatttagc agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca
    12121 aactatgctg ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct
    12181 ggagttgccc gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa
    12241 acataaaccg cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc
    12301 ccacggtctg gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg
    12361 cggatgcagc ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga
    12421 cgccagttgg tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt
    12481 cggctattcg ctcggcggta atatgctggc ctgtttgctg gcaaatgaag gcaatgatct
    12541 cccggttgat gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca
    12601 tatggaaaag ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa
    12661 tgccgcgcgc aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa
    12721 atcggtacgt cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc
    12781 cgacgctatc gactattatc gtcagtgtag cgccatgcca atgctgaacc ggatcgccaa
    12841 accgacgctg attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccaaa
    12901 accggaaagt ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg
    12961 ctttattggc ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg
    13021 gttaacaacg tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa
    13081 acgctggaaa atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat
    13141 gagcgtacac ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg
    13201 gtgctggtat ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc
    13261 gaataacctg caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta
    13321 tgtctgccaa acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca
    13381 ccagcctcgc gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag
    13441 gcgacagttt tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg
    13501 acgccgggcg gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac
    13561 aaaccttcat tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct
    13621 acgacgaagc cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac
    13681 cagaacccac tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc
    13741 ataacgttgc gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt
    13801 ggattcaaaa actgatccgc gacaccagcg agcgcgggca ctcacgagaa acagtgatgg
    13861 actcggtagt gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca
    13921 cccatcttaa cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag
    13981 gtatcccgtc gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg
    14041 atttcccttg gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag
    14101 tggtacctgg cggcaaaatg gggctggcaa tggaattaat tatgctgccg ctggtgcaac
    14161 gattgatgga aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc
    14221 ggcttcacaa cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc
    14281 accgcttttt ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca
    14341 cgcgcaaccg ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc
    14401 gtaaacaggc gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc
    14461 acatcctgac gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc
    14521 gccatcaaca ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa
    14581 atctggtgac cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc
    14641 gcttgcattt ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa
    14701 atcgcgcaac ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg
    14761 aaagctatgc taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca
    14821 aaggacgtca cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat
    14881 atttcggcaa tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga
    14941 taaccgcgca tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct
    15001 cattgccaca ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg
    15061 gaaacgctgt actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt
    15121 aaagaaatga tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt
    15181 gaagagggcc aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa
    15241 atttcgtaca aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg
    15301 tctgcacaga tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc
    15361 ctcgacgtga cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct
    15421 atgactcacc cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc
    15481 ggctgttctc gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc
    15541 tccgcacacg gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg
    15601 ttacctggta gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg
    15661 atatcaacta tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa
    15721 tgcttggcga attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg
    15781 cgggccttga tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt
    15841 ttgccacctg tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt
    15901 tgctgaccgg attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa
    15961 aattgcttcc tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc
    16021 cggtctggga accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact
    16081 ggttttggtt ctggatctgg cgcgaacaac cgctgcgcga gtcactacgt ctgctgtacc
    16141 gtgaactggc agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg
    16201 aaaaagcgct gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt
    16261 gctatcagca aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc
    16321 gtattttcca ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg
    16381 aagaggtgca aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc
    16441 aaaccgtcgc cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa
    16501 cgcgttttac gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg
    16561 ataatccggt tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca
    16621 cccaaaaacc gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttgc
    16681 ttccggcact gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac
    16741 tcagtgtgat gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt
    16801 actggatcct gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga
    16861 ggattgtgaa tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc
    16921 actttaaaat tcccgaaagt tacaccctga cgttgatgct gattaccacc ctcgccagct
    16981 acctgatatt gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt
    17041 ataccctgca actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata
    17101 ccattattgg ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga
    17161 gcgggttatt gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct
    17221 tgattctgag cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc
    17281 aggcgcataa cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca
    17341 gccattatct ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata
    17401 ttaatgccat gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag
    17461 agtatttaca gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg
    17521 aaccgggtag ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag
    17581 gcgcggcagg tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc
    17641 acaccatttc gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca
    17701 agttgcggga ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca
    17761 tccgacatat tttgcctgat acgtcttatt tacgccccaa ccaccttcgc caccgcgtgg
    17821 gcgaaacgtt gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa
    17881 cgcatcacat ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg
    17941 aaatcacgcg cccgaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata
    18001 ccgcgaatat cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa
    18061 cgctggcgtt tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc
    18121 gcgcccgcta ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga
    18181 tgaaacgcag aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct
    18241 aacgctttcg cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa
    18301 tcgccagtcc gccccatccc gcactgcact tcatcaaaca ccaataatgc ctgatgttga
    18361 tcgcacagct cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg
    18421 ccctggatcg gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca
    18481 tggagatcgt taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa
    18541 tattttggct gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg
    18601 aaggcaataa ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt
    18661 ttaaaggcgg tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac
    18721 gtcgcctcaa tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg
    18781 atatgccaca gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg
    18841 cccaacgccg taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc
    18901 cagattcggc tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg
    18961 atcacttcat cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca
    19021 ccctgttacg cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcactg
    19081 aatggcaatc ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct
    19141 aagaatactt tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc
    19201 cataatctcg cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg
    19261 ttccaccacc agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac
    19321 gccctcaccg ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac
    19381 aactttgccg ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg
    19441 caagcgcccg gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca
    19501 ggggccaggt gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa
    19561 cgcatcgttg cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt
    19621 ccaggtaaaa gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa
    19681 ccgcgctatt ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca
    19741 gtgcggtgag atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga
    19801 taccctgcca gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct
    19861 gtgccagacc acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg
    19921 ccagccgctc gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat
    19981 cctgcatcag ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc
    20041 cctgataaat atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct
    20101 ggcgatggat agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg
    20161 cagcacgcag cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt
    20221 tcagccggtt acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga
    20281 atttatcgct catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta
    20341 agagtgacta aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta
    20401 cgctgaagct cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc
    20461 cctgaacgtg tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca
    20521 gaagcgcggg caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga
    20581 taactaccgg ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg
    20641 aaatcttatc ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat
    20701 cacgctgacc atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg
    20761 cgctgtcttt gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt
    20821 tatcggcttc atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac
    20881 cgccgccctg aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac
    20941 cgctgttcac ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct
    21001 ccagttcgat gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca
    21061 ttgctgcggg agaaagagca gaaagagcag aaagagcgaa aacagcagcc atcgccgcca
    21121 gggtcgattt gaacatgaga tttccttaac aaacagagca gaaaaaaagc aagtgaagag
    21181 attctaaata gccttcaaga acaaggccat ccctttacct aattttacgt atctgaaata
    21241 tatgtaacct gacgatcact ttttattccg tgatcaaaat cacctcttaa aatgcaattt
    21301 agcaaccgat tgcaataaaa catttaaaca gatcataaaa tcacctaaaa tcgcccgctc
    21361 gcggcatatc ccgctatgcc attttatttt tgaacgcagg ccaatcatga ctaacagcaa
    21421 tcgcatcaag ctcacatgga ttagctttct ctcctacgca ctgactggtg cgttggttat
    21481 tgtcaccggg atggtgatgg gaaatatcgc cgattatttc aatctgcctg tttccagtat
    21541 gagtaacacc ttcaccttcc tcaacgccgg cattttaatc tctatcttcc tcaacgcctg
    21601 gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt ggctttctcc tgatggtgct
    21661 ggcggttgcc ggtttgatgt tcagccacag cctggcgctg ttctcgacgg cgatgttcat
    21721 tctcggggtg gtcagcggca ttactatgtc gattggtaca ttcctgataa cacaaatgta
    21781 tgaagggcgt cagcgcggtt cccgcctgtt atttactgac tccttcttca gtatggctgg
    21841 gatgattttc ccaatgatcg ccgcgtttct actggcgcgc agcattgagt ggtactgggt
    21901 ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt ctgaccttcg gctgtgagtt
    21961 cccggcactg ggcaaacgtg caccaaaaac ggatgctccg gtagagaaag aaaagtgggg
    22021 gatcggcgta ctgtttctct ctgttgcggc gctgtgctac atcctcggtc agttaggttt
    22081 tatctcctgg gtgcctgagt atgccaaagg cctgggcatg agcctgaacg acgcgggcac
    22141 gctggtgagt aacttctgga tgtcatacat ggtcggcatg tgggcgttca gctttattct
    22201 tcgcttcttt gatttgcaac gcattctgac cgtactggct ggtctggctg cgattctgat
    22261 gtacgtcttt aacaccggaa caccagtaca tatggcgtgg tcaattctcg ctctgggctt
    22321 cttctccagc gcgatctata ccaccatcat caccctgggt tcacagcaga ccaaagtacc
    22381 gtcgccaaaa ctggttaact ttgtcctgac ctgcggaacc atcggtacta tgttgacctt
    22441 tgtggttacc ggcccgattg ttgaacatag cggtccgcag gcggcactgc ttacagcaaa
    22501 cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta ggtttcgtca gccgtcaccg
    22561 tcagcataac accctgacct ctcattaatt gctaatgccg gacggcacta tcgtcgtccg
    22621 gccttttcct ctcttccccc gctacgtgca tctatttcta taaacccgtt cattttgtcc
    22681 attttttgca caaacatgaa atgtcagaca attccgtgac ttaagaaaat ttatacaaat
    22741 cagcaatata ccccttaagg agtatataaa ggtgaatttg atttacatca ataagcgggg
    22801 ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc gaggcaaaaa tgagcaaagt
    22861 cagactcgca attatcggta acggtatggt cggccatcgc tttatcgaag atcttcttga
    22921 taaatctgat gcggccaact ttgatattac cgttttctgt gaagaaccgc gcatcgctta
    22981 tgaccgcgta cacctctcgt cttacttctc tcaccacacc gccgaagagc tgtcgctggt
    23041 gcgcgaaggc ttctacgaga aacacggcat caaagttctg gtcggcgaac gcgctatcac
    23101 catcaaccgt caggagaagg tgattcactc cagcgccgga cgtaccgttt tttatgacaa
    23161 gctgatcatg gcaaccggtt cctacccgtg gatcccacca atcaaaggtt ctgatactca
    23221 ggactgcttt gtctatcgca ccattgaaga cctcaacgcc attgaatcct gcgcccgtcg
    23281 cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt ctggaagccg ctggcgcgct
    23341 gaaaaactta ggtattgaaa cccacgttat cgaatttgcc cctatgctga tggcggaaca
    23401 gcttgatcag atgggtggcg agcagctgcg tcgcaaaatc gaaagtatgg gcgtgcgcgt
    23461 tcacaccagc aaaaacaccc ttgagattgt gcaggaaggt gtcgaagcgc gtaaaaccat
    23521 gcgttttgcc gacggcagcg aactggaagt cgactttatc gtcttctcta ccggtatccg
    23581 tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt gctccgcgtg gggggattgt
    23641 cattaatgat tcctgccaga cttccgatcc ggatatctac gccatcggtg aatgcgcaag
    23701 ctggaacaac cgtgtatttg gtctggtagc accaggctac aaaatggcgc aggtcgccgt
    23761 tgaccatatt ctgggtagcg aaaacgcctt tgaaggtgct gaccttagcg ccaagctgaa
    23821 actgctgggc gtagacgtag gtggtattgg tgatgcgcac ggtcgcacgc ctggcgcacg
    23881 tagctatgtt tacctcgacg aaagtaaaga gatctacaaa cgcctgattg tcagcgaaga
    23941 caacaaaacc ctgctcggtg cggtactggt gggcgatacc agcgactacg gcaacctgct
    24001 gcaactggtg ctgaacgcta tcgagctgcc ggaaaacccg gattctctga ttctgccggc
    24061 gcactcgggt agcggtaagc cgtctatcgg tgttgataaa ctgccggaca gcgcgcaaat
    24121 ctgctcctgc ttcgacgtca ctaaaggtga tctgattgct gccatcaaca aaggctgcca
    24181 cacagttgcg gcgctgaaag ctgaaaccaa agcgggtact ggctgcggtg gctgtatccc
    24241 gctggtcact caggtactga acgcggaact ggcgaaacag ggcatcgaag ttaacaacaa
    24301 cctgtgcgaa cactttgctt attcgcgtca ggaactgttc catttgatcc gcgttgaagg
    24361 cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa ggctacggtt gtgaagtttg
    24421 taaaccaacc gtcggttcgc tgctggcctc ctgctggaac gaatacattc tgaagccgga
    24481 acatactccg ctgcaggatt ctaacgacaa cttcctcgct aacatccaga aagacggcac
    24541 ctactcggtg atcccgcgtt ctccgggcgg tgaaatcacc ccggaagggc tgatggcagt
    24601 gggtcgtatc gcgcgtgaat ttaatctcta caccaaaatc accggctccc agcgcctggc
    24661 gatgtttggc gcacagaaag acgatctgcc ggaaatctgg cgtcagctga ttgaagctgg
    24721 cttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg gcgaaaacct gcgtgggtag
    24781 cacctggtgc cgctacggtg ttggcgatag cgtcggcctc ggcgtggaac tggaaaaccg
    24841 ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt gtctccggct gtacccgtga
    24901 atgttcagaa gctcagggta aagacgtggg tattatcgcc actgaaaaag gctggaacct
    24961 gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg gatctgctgg cagcggatat
    25021 cgatcgcgaa acgctgatca aatatctcga ccgcttcatg atgttctaca tccgtaccgc
    25081 cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc gaaggcggca tcgattacct
    25141 gaaagcggtg atcattgacg acaaactggg gctgaacgct catctggaag aagagatggc
    25201 gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg gtcaatacac cgtccgcgca
    25261 gactcgcttc aaacacttca tcaacagcga caagcgtgac ccgaacgtgc agatggtgcc
    25321 agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt atcccggtaa ctctggtgga
    25381 ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg atgacatcct gcctgaaacc
    25441 ggcgtctgcg cactgttagg tgacaagcag gtagcgattt tccgcccgta tcacagcgac
    25501 caggtgtttg cgatcagcaa cattgatccg ttcttcgagt ccagcgtgct atcacgcggg
    25561 ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc cgctgaaaaa acagcgtttt
    25621 cgcttaagtg atggcttgtg catggaagac gaacagtttt ccgtcaaaca ttacgaagca
    25681 cgagtgaaag acggcgtggt gcagctgcgc ggttaatgtt ttaacgggag gcgcaatgcc
    25741 tccccttttt gcatggtcct gtaatgacct tcggtatatt gcaggacatt ttttaaactt
    25801 tttgttttat tttttgtttt tattttttaa aggataatca aatgtttaca gacactatta
    25861 ataagtgtgc ggctaatgct gcgcgcattg cacgcctgtc ggcaaataac ccgctcggct
    25921 tttgggtcag ctccgccatg gcgggcgcat atgtgggtct tgggatcatc ctgattttca
    25981 cgctcggtaa tttgctcgac ccgtccgtac gtccgctggt gatgggcgcg acctttggta
    26041 tcgccttaac gctggtgatt atcgccggtt ctgaactgtt caccggacac accatgttcc
    26101 tcacctttgg ggtaaaagcg ggcagcatca gccacgggca aatgtgggca atcctgccgc
    26161 aaacctggct gggtaacctg gtcggttccg tcttcgttgc catgctctat agctggggcg
    26221 gcggtagcct gctgccggta gataccagca tcgttcactc cgtcgcgctg gctaaaacca
    26281 ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg taactggctg gtttgcctgg
    26341 caatctggat ggcgctgcgc actgaagggg cggcgaaatt tatcgctatc tggtggtgtc
    26401 tgctggcatt tatcgcgtcc ggctacgagc actctatcgc taacatgacg ctgttcgcgc
    26461 tctcctggtt cggcaaccac agcgaagcct acacgctggc gggtattggt cataacctgc
    26521 tgtgggtgac gctgggtaat actttatcag gtgccgtatt catgggattg ggttattggt
    26581 atgctacgcc gaaagcgaat cgtccggttg cggacaaatt taatcaaact gaaacagctg
    26641 ccggttaatt actaaggggt ttttacgtgg atcatttgcc tatattttgc caattacgcg
    26701 atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga acgcaaagca aggttgctgt
    26761 tagacgcagg cgctcgctta acggtgaatg cattagcgtt tattccacag ttcaccgcat
    26821 gggcagatgc aggcatgtta accctcgtcg aagggccatt tgatgaaagc cttctcgaca
    26881 cctgctggct ggcgattgca gcgacggatg atgacacgct taaccagcgc gtcagcgaag
    26941 ccgctgaagc tcgtcgcatc ttctgtaacg tagttgatgc gccgaaagcc gccagcttta
    27001 ttatgccgtc gattattgac cgctcaccgc taatggtagc ggtctcctct ggcggcacct
    27061 ctccggttct ggcgcgcctg ttgcgcgaaa aactcgaatc actgctgccg ttgcatctgg
    27121 gccaggtggc gaaatatgcc gggcaattac gcgggcgagt gaaacaacag ttcgccacga
    27181 tgagtgagcg tcgccgtttc tgggagaaat tgttcgttaa cgaccgcctg gcgcagtcgc
    27241 tggcaaacaa cgatcagaaa gccattactg aaacgaccga acagttaatc aacgaaccgc
    27301 tcgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc gggcgatgcc gggctgctga
    27361 cgctgaaagg actgcaacaa attcagcagg cagatgtggt ggtctacgac cgtctggttt
    27421 ctgacgatat tatgaatctg gtacgccgcg atgctgatcg cgttttcgtc ggcaaacgcg
    27481 cgggatacca ctgtgtaccg caggaagaga ttaaccagat cctgctgcgg gaagcgcaaa
    27541 aaggcaaacg cgtggtgcgg ctgaaaggtg gcgatccgtt tatttttggc cgtggtggcg
    27601 aagagctgga aacactgtgc aacgcgggta ttccgttctc ggtggttccg ggtattaccg
    27661 cagcttctgg ttgctctgcc tattcgggta ttccactcac gcatcgcgat tatgcccaga
    27721 gcgtacgctt aattaccgga cacttaaaaa ccggtggcga gctggactgg gaaaacctgg
    27781 cggcagaaaa acagacgctg gtgttctata tggggttgaa tcaggccgcg actattcagc
    27841 aaaagctgat tgaacacggt atgcctggcg aaatgccggt ggcaattgtc gaaaacggaa
    27901 cggcagtcac gcagcgcgtg attgacggta cgctcacgca actcggtgaa cttgctcagc
    27961 aaatgaacag tccatcgcta attattattg gtcgggttgt tggcctgcgc gataaactga
    28021 actggttctc caatcattaa tttaacccgg gccagagaat tctggccttc ttaatgactc
    28081 tttttatata ttcagcaaat aaaacataac cccttaataa atattatctg ctaatgggtt
    28141 atattcaccg ttacagcgac ttacaaaatt aagtcattcg ctcactgacg ccaattaaat
    28201 attcctgtat cgttcttaac gagcagtctg cttatattca tataatcaat gaatattaat
    28261 taatatataa tacataggga atgtaaatga acaaatttat taaagttgca ctggtaggtg
    28321 cagtactggc tacgttaact gcatgtactg gtcatattga aaaccgtgat aagaactgct
    28381 cttacgacta cctgctgcac ccggcaattt ctatttctaa aatcattggc ggttgcggtc
    28441 ctactgcaca gtaagtctct gacagaaatc ggctaacacc ggtttaattg cccgacgccc
    28501 tctggatttt ccgagggcgt atttgtttct atgtccttct tgcccccgcc cgtaaaaata
    28561 ttttcacttt aaattcaatt ttataactac atccattagc cacagcgcga tcctgtgcga
    28621 aattttgtga tcttcctcca cattacacaa cataatatgt tgttatattc atcatgcatt
    28681 gtcatgttac cttttaaatg actgcaaact ctcccctaca acgtattgga caagaaaaag
    28741 gtatcgctat gggaagccag gaactccaac gcaagctcag attttgggcc gttcttgcaa
    28801 tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa gtggcaaaag
    28861 cagcgggcac gccgtggctt acggtgctcg cgtttgtcat tggcgggtta attgtgatcc
    28921 cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc ggaaaatggc gcagattatg
    28981 tttatctgaa aaatgccgga agccgaccgc tggctttcct ctccggctgg gccagcttct
    29041 gggccaacga tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc aatcttggct
    29101 ttttaacgcc tatcgatccg ttgctcggta aatttatcgc cgccggatta attatcgcct
    29161 ttatgttgct acacctgcgc tccgttgaag gcggcgcagc gtttcagacg ctaattacca
    29221 tcgccaaaat tatcccgttc actatcgtca ttggccttgg gatcttctgg tttaaagcag
    29281 agaattttgc cgcccttacc accactgcga ttggcgcaac gggcagcttt atggcgctgc
    29341 tggcggggat ctctgccacc agttggtcgt ataccggcat ggcctctatc tgttatatga
    29401 ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc gctgattggt tcctgtctgc
    29461 tggttctggt gctctacacc ctgctggcgc tggtgatttc cggcctgatg cccttcgaca
    29521 aactcgccaa ttctgaaacg ccgatttccg acgccctgac ctagatcccc gcactcggca
    29581 gcaccgctgg gctctttgtt gccatcacgg cgatgatcgt cattcttggt tcgctttcca
    29641 gctgcgtgat gtaccagccg cggctggaat acgcgatggc gaaagacaac ctgttcttta
    29701 aatgcttcgg ccatgtgcat ccgaaataca acacgccgga tgtctccatc atcctgcaag
    29761 gggcgctggg gatcttcttc atcttcgttt ccgatctcac cagcctgctg ggttatttca
    29821 ccctggtgat gtgtttcaaa aataccctca ccttcggctc catcatctgg tgtcgtaaac
    29881 gcgacgatta caaaccgctg tggcgtactc cggctttcgg gctgatgacc accctcgcca
    29941 ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt cccggcctta
    30001 tctgcgccgt catcgttatt gctactggtc tgcctgctta cgccttctgg gcgaagcgta
    30061 gccgccagct caacgctttg tcgtaatgtt atctgaagaa aataaaatgt tggatattga
    30121 taaaagcacc gtggactttc tggtcaccga aaatatggtt caggaagtgg aaaaagttct
    30181 cagccatgac gttccgctgg tgcacgccat cgtggaagag atggtgaagc gcgacattga
    30241 tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg gcgcaaacgg cgaaacatct
    30301 ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc ggctgggagt tctgcgataa
    30361 caccccgtat cgcctcgacg atcgttgcgc ggtgattggc gtttctgact acggtaaaac
    30421 ggaagaggta atcaaagcgc tggagctggg ccgggcctgc ggcgcactca ctgcggcgtt
    30481 caccaaacgc gcggacagcc cgattacctc ggcggcggaa tttagcattg attatcaggc
    30541 cgactgtatc tgggaaattc acctgctgct ctgctacagc gtggtgctgg agatgatcac
    30601 ccgcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac gatctcaagc agttgccgaa
    30661 tgcgctcggt catctggtac gcacctggga agaaaaaggc cgccagcttg gtgaactggc
    30721 cagccagtgg ccgatgattt ataccgttgc tgcgggtccg ctgcgtccgc tgggttacaa
    30781 agaaggcatt gtaacgctga tggaatttac ctggacgcac ggctgcgtga ttgagagcgg
    30841 agagttccgc catggcccgc tggagattgt cgaaccgggc gttccgttcc tgttcctgct
    30901 cggcaatgat gaaagtcgcc acaccaccga acgcgccatt aactttgtta aacagcgtac
    30961 tgacaacgtg atcgtcatcg attacgccga aatttcgcaa gggctgcacc cgtggctggc
    31021 accgttcctg atgttcgtgc caatggagtg gctctgctac tacctgtcta tttacaaaga
    31081 tcacaacccg gatgaacgcc gctattacgg tggtctggtg gaatattaat ccctctcccg
    31141 gcccggcaac gggccggatt tacgcaagga gttacccgat gaaaacaggt atgtttacct
    31201 gcggccacca gcggctgcct attgaacacg catttcgtga tgcaagcgag ctgggttacg
    31261 acggcatcga aatttggggc ggtcgcccgc acgcgttcgc gccggactta aaagcgggcg
    31321 gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat gccgattatc ggttacacgc
    31381 cagaaaccaa cggctatccg tataacatga tgctgggcga tgaacatatg cgtcgtgaaa
    31441 gcctggacat gatcaagctg gcgatagata tggcaaaaga gatgaacgcg ggttatacgc
    31501 tgatttctgc ggcccacgcg ggctatctca cgccgcctaa cgttatctgg gggcggctgg
    31561 cagagaactt aagcgaactg tgtgagtacg cggaaaacat cggcatggat ctgatcctcg
    31621 aaccgttaac gccgtatgaa tcgaacgtcg tgtgtaatgc taatgatgtg cttcatgcgc
    31681 tggcgctggt gccttcgccg cgcctgttca gcatggtcga catttgcgcg ccgtatgtcc
    31741 aggcggaacc ggtgatgagt tatttcgaca aactgggcga taaattacgt catctgcata
    31801 ttgtcgacag cgacggggcc agcgacacgc attacattcc tggagaaggc aaaatgccgc
    31861 tgcgggaact gatgcgcgat attattgatc ggggctatga gggttactgt acggtggagc
    31921 tggtgacgat gtatatgaac gagcccagac tctatgcccg ccaggcgctg gaacgctttc
    31981 gcgcgctgct gccggaggat gagagatgaa aaccctggcg acaatcggcg ataactgcgt
    32041 cgatatctac ccgcaactga ataaagcgtt ttctggcggt aatgcggtca atgtggcggt
    32101 gtactgcact cgctacggca tacagccggg atgcattacc tgggtgggtg acgatgacta
    32161 cggcacaaag ctgaagcagg atctcgcccg catgggcgtc gatatcagcc atgtccatac
    32221 gaaacacggc gttaccgcac aaactcaggt ggaactgcac gacaatgatc gcgtttttgg
    32281 cgactacacc gaaggcgtga tggccgactt tgccctgagt gaagaggatt acgcctggct
    32341 ggcgcagtat gacattgtgc acgcggcaat ctggggacat gcggaagacg cattcccaca
    32401 gctgcacgct gcgggcaaac ttaccgcttt cgacttctcc gacaagtggg acagcccgct
    32461 ctggcagaca ctggtgccgc atctcgattt tgcctttgcc tccgcaccgc aagaagacga
    32521 agcgctgcgt ctgaagatga aagcgattgt tgcccgtggc gcaggaacag tgattgtcac
    32581 gctgggtgaa aacggcagca ttgcctggga tggcgcgcag ttctggcgtc aggctcctga
    32641 accggtgacg gttatcgaca ccatgggtgc cggagattcg ttcattgccg gattcctttg
    32701 cggctggtct gcggggatga cattaccgca ggcgatggcg cagggaacgg cgtgcgcggc
    32761 gaaaaccatt cagtaccacg gtgcctggta ggtataacgt tggcgtgagc atcttcacgc
    32821 caacgtgctg ttacttgccg gaaaacgacc ctataatccg agtaattcat tctttatttc
    32881 agggtcgatt atgtcagcta cggaccgcta ctctcatcaa ctcctctacg ctaccgtccg
    32941 ccagcggctg ctggatgata tcgcgcaggg ggtttaccag gccgggcaac agatccctac
    33001 cgaaaacgag ctttgtacac aatataacgt cagccgcatt accattcgca aagccatcag
    33061 cgacttagtg gcagacggcg tactgatccg ctggcaggga aaaggcacct ttgtacaaag
    33121 ccagaaagtt gaaaacgccc tgcttactgt cagtggtttt accgattttg gcgtctcaca
    33181 aggcaagtcg acgaaagaga aagtgatcga acaggaacgg gtcagcgccg cgccgttttg
    33241 cgaaaagctg aacatccccg gaaacagcga agtgttccat ctctgccggg tgatgtatct
    33301 cgataaagag ccgctgttta ttgatagttc atggatcccg ctgtcgcgtt atcctgactt
    33361 tgatgagatt tacgtcgaag gaagctccac ctatcagtta tttcaggagc gttttgacac
    33421 gcgagtggtc agcgacaaaa agaccatcga tatctttgcc gccacccgcc cgcaggcaaa
    33481 atggctgaaa tgcgaactgg gcgaaccgtt gtttcgcatc agcaaaatcg cctttgatca
    33541 gaatgacaaa ccggtgcacg tctccgaact cttctgccgc gccaatcgca tcaccttaac
    33601 gattgataat aaaagacatt aaccgtaggc cggataagat gcgccagcat cgcatccggc
    33661 gatgctggcg cactgaattt tacatcccgc acgttcccct caccctaacc ctctccccaa
    33721 aggggcgagg ggaccgttca gtgcggaatt tagcttgatt gccgaacggt gctatccagc
    33781 attaaatact cgcaatactc tcacgcaaaa tccgcagcac cgtctcttca ccgctgcgat
    33841 tcgggttaat gcgaatcgca caatgttcga gttgtggatt cgcctggcga aacgttcctg
    33901 aaagtcgata gaagagcggc gggatttcat atttcgactc tgcaccgacc gggtaaggca
    33961 acgcgccacg cttttgcgct tcttccagca ccctggcggc aatcagctga tgaaactcga
    34021 caatcaacac cttcgactgc gcattggcaa tgacggcgct tttcacttcc gccaccgcgc
    34081 cgccgttaag caaagccagc aaccgttcgg atactcccgc ctgcaccgca tgcatcactg
    34141 gcgcgaacac cagaccacgc aagacttcca gcgcctgtga gccctggatc tggctaccgc
    34201 cggagtaaag cgtggcgcga atccgactaa taacatcggc atcgccaacc actgcaccaa
    34261 cgccctccgg cccaaacagc ttgaagcagg agaatgtcga gacattcgcg ccgcattcac
    34321 agccgattcg cgctaccttc atcaccgcat agttatcatc ggttaacgct ggaacacctg
    34381 ccgcgcgcaa cgttgccagc acatctgcca gcacatagct gtcctgcggc tgctggcgcg
    34441 tatgctgcac cagcgcggca tcaggttgtt gctcgtcgac gacctgcttc agtgccgaca
    34501 ggtcattgaa atcagcagta ataagcgtca gccccatctg ctcaataata acccgtgtcg
    34561 tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc cggtttgagc aaagccgcca
    34621 gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag cgccgcggcc tgtgcgtgaa
    34681 atgcatcagc cagcacctgc tccacccgtt gggtaatacg cggttgattc agacttggag
    34741 ttaaccctaa atcaccgcca gcaagaaact cgctgcctgg aaaatggcgg caaatagtgt
    34801 ccaccagcgc aaactgcttt tgctgcgctt cagcaagagt caggctttgc aaaggaaacg
    34861 tcttcatagg tgccttcctt aagccggaac gaacaggcca agccagtaaa gaacattcag
    34921 cagaataccg gtgatcatta ctgccacgac cggtgccgcc attttctgta ccggacggcc
    34981 cagggattcg ttgaggaagt aaatggcaac cgcgatagag aatccggtat aacccgccat
    35041 cttaattgct gcgaaaatcg aaccgaccag cagcgccact tccatcagca tattcatggc
    35101 gttacggatg ttatccgacg cattacgcac cgacgggtag cgtcccagcc atttgccgat
    35161 cgaacgaagc agcaagactt ccgccgaaat aaccactgcg cctaataccg ctgcaaccat
    35221 cggattcggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa ctgcatacac
    35281 acccgttgct aaggcggtgg tggcaatcaa cggcacaaac cccagtccgc gcataaattc
    35341 tgccagagcc gcctgattaa tcagcgtttg cgattgttcc ggcgtcacgc ctgcggaata
    35401 cgctttctcc agtgtgaaga tcgaaacttc gctaccagca aaaatcttca tactggcaac
    35461 ggcggcaatc aatgctccca cgatggcgat ataaggtaag tttttgataa tccgcgacgt
    35521 gcgctcttca aacaccgaaa gcccgctggc atcgatgtca ttttcatcac gatgacgcag
    35581 gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca ataaagattt cgatggattc
    35641 agggttaaga tgtgggaaat agcgcacgac taccacacgg gtcatcagaa caaccacggc
    35701 ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc gccaccagcg ggaacagtgc
    35761 aaaagcagaa accaccggcg agcttaattc cccgaggcta cctaatacat ccaccggcag
    35821 cgcggtcagc agctggttaa ctggcaacag gcaagtaagg atcaacacgc cccagatagc
    35881 gccaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat cggtaggaag
    35941 aaagagtaac catgcgttga gcagcccggt tttcagggta aaagagatcc ccaccgaggc
    36001 cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacggt tcatatagcc
    36061 ttcaatcagt tgcggcagga tcgggtggat gccgtcatga aaaacagccg ccgagcgatg
    36121 cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa tatacagatc
    36181 catagtcgtg ccttttattt cagatggtta atcaacatcg gaatcgcgtg ttcgacgtgc
    36241 tcaacggaaa ggccaaacgc cactttccct tcagcgatca ttttggcgat atgctcgtct
    36301 ttcgctttaa tgcctggttt ggcaatggtg caacttttgt tatagccaat caccgcgatg
    36361 gcaatcgaca atgccgcgcc cgcaccggta ttacacgcgc caatgtaata atctagttgc
    36421 ccggatttca ccttcattgc cgcttccatg tcgttgtgaa tgaaaacttc aaaacagcca
    36481 ggagccgtcg cttcaatggt ttttttaatc tgctcacgct gtaagcctgc aacgccaatc
    36541 tttttcatga gtctgtcctt attggaaaaa ttgagaggga ttttcacgta acatcacatc
    36601 cacatcggcc tgactgaatc ctgactggcg caattgcgga ataaaggtgg tcaataaata
    36661 gtcatagcca taaccaccat tggcttttaa atgggagcgg cgcgtaatat ccatcgacag
    36721 catgacgcgg ttcagcaacc cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc
    36781 gtccgggtag taactgttct taccaatggt gtcgaactgc acgtacgcgc cgagatcgat
    36841 catcttcaaa atgttgtcga ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga
    36901 aagatcaact ccgtgggctt gcaacagagc cagttgttcc agccccatgg tgctgaacga
    36961 cgtatgcgtg gagatcgggc gtccggtctg gttatgcgcc agcgcagcgg caataaatac
    37021 cttctcttcc agcggcgtaa tctttccttc gctggagccg atctccgcga tgatcccggc
    37081 tttcagctcc gtgccatcga taccctgttc aatttcatcg accatctcct gcgccagttc
    37141 ctgcacgctg cgggtcacca catgttccgg gaaaaacgca tcctggtaat aaccagtaca
    37201 ggccaccacg ttgatcccgg tttcgcgcat tacatcgagc ataaattgcg cattgcgccc
    37261 catgtaacgg ttggtcatct caatcacatt acgtacaccc cgggtcatca ggtcgttcat
    37321 ctcctggcaa atgaacgcat actgatcaag gcggcagtcc acgttgtttt taaagccgga
    37381 gagatcaata tgcagatgct catgggccag ggtgtaaccc gtcggatcaa aactcatagg
    37441 gtgtctcctg caaaccggag ggaggaaaga aaagagcgac cattttgtgg cggtggcgca
    37501 cggaaaaatt cacacacggt agctcccaca tcagaaagcg tggtgcgcac accgagctgc
    37561 gtggcgacca gcccttgctg ataaaccagc actggcacca cttcacgggt atggtggctg
    37621 tgaccaatgg tcggatcgtt accgtgatcc gccatcacga ccaggcaatc atctggctgc
    37681 atcgcctcaa caagccgggc aaggttacgg tcaacgacct gcaaacgttc ggcataacgt
    37741 gcgacgtctt ctgcatgacc agcgaggtcg gtttcctgga tattgatgca aataaacgcc
    37801 gtcggatggg tgttaaattc gtcgagggtg atatccataa tccgctggct atccaccagg
    37861 ttttgccagc tcacaccata aggattgctg acgatatctg ccaccttacc caccagcacg
    37921 gttggcacgc ccgcttcata cagtttttgc ggcacctgca ctttttcatc aacaccgtag
    37981 cccatatgca cgacctggaa gccgttgtca taagcgccag aacgcggcgc attaatacca
    38041 ataaagcgcc cttctttgct ttctgcggca tcgagaatac gctgactgtt ggttaacagg
    38101 ccgccaaatg taatgacccg accgacctgt acctggtcac gcacgatacg accaattttg
    38161 attgcgtcgt caaaagagat cacagagaga ttggcggtaa tgttatagac ttggcctaaa
    38221 tccgcctcga gattatcgcc aatcgcaacc gcctgattga ccgacagaaa ttgcagatca
    38281 tcgccacggc gctccacctg ccagccagcg gaaactaatg cctgctcaac acggtcaatc
    38341 acatcgcgaa aaggcatccg cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc
    38401 cccataaagg tatcgccacc ttcatgttgc agctctgcca cgccccaggt tgcggaatct
    38461 gacggctgca tatcgcctgg cgcataaccc aatgtgttga ttagccccag cttctccagc
    38521 gttggtagct gcaaatgcgg caactggctc aggatgtgac cacaggtatt cgccccggca
    38581 tcttgcgggc gcaccagcgt gacatctttc attgccccta cgccaaagct atcaatcact
    38641 aacaccacaa atcgcgccat ttatgcctcc agagggttgc cgaggctgtc ataacgacca
    38701 actatttctg gttcgccgcg ctgaataccg gacaccagca ccacatcgct acgggtgaca
    38761 aaaatctgcg tgcgaaaaca gagcaccact gcactgctca ccggatactc gcctgccagc
    38821 ggcagggtgt agtcgatact gctgtcatcc acagttttga gattggtttc agtaatcttt
    38881 tgattttctg gcgtaaacac cagcgcatgt tgcgcatgac cacgacggta gtaaccgccg
    38941 ccgtagcagt agctgtcgcc acggaaatga tgggagattt cacttaacca gagcatcgcg
    39001 atacgttcag gctgatcgcc ctgctggttt gccggaatag taccggtcag cgcatgaccg
    39061 ggttcggcat gagtcgcacc gtattccgcc agcaatggca gcgaagtgca gctggtcgct
    39121 gaaggtacgt tcagttgctc aatcgcaata ccagatttcg ccagttgatc ccgtgcttgt
    39181 accagcgtgt gaagattcgg tgttggcaaa actttcccag cagcctcatc ccaaagcagg
    39241 caagggaaat gggttagtcc ggctaaatgc agccccggca gtttcttaat ttcagcaatc
    39301 acctcgtgca gcgaatgctg tacaaaaccg ctctcctgcc ccggataaag aaaatcatcg
    39361 tcgctataaa ctttgagcag cacaggctgg acccgtccgg ccttaaccgc tgccgcagaa
    39421 acttcccgcg ctttgtcgag agtaaacacg gtgatgatgt cggtgccctg ttcaacggcg
    39481 tcagcaacct gatgacaagg gatttgtacc agatgcccct gatgcgccac tggcaaacca
    39541 gcgcggcgca tgactcgcgc ctctttgtaa tccaccgcca caatgccgct gtagcctaat
    39601 gccagcaatt tttccgccag ccacggatta cgaccaaatt gcttggtcat cagatacagt
    39661 tcaatgccgt aaagccgcgc cgtctcaatg agccgcttac cgttttccag tatctgatcc
    39721 acgtcgatca cccagctgtc cggggcgatt ttgccctgct gccacaaact tagtgcggcg
    39781 gaaatcagcg ccgggttctg gcgtttcaat gcttctacaa acatcttgtt gcctcgtttc
    39841 tgttccttga attaaatatt catttttttg aatatttagg ttgtgcgacg tggatttgtc
    39901 gggagcttca aacctcttcg ttagcagcca tccataatcc ataaagattg gccagaaaat
    39961 agccttcttc acacgggtta acttccagag cgaattcctt caacaactcc tgatgcaatt
    40021 gcacaacggc ttgccagtgg ctggattgcg ccagttccgc cagcaactcg ttatccagcg
    40081 gctctatttc ttcaccacgg cgactgcgca tcagtgcact cgccatatgt gtcatcgcca
    40141 tcgttccttg ctcactgcgc accggcagat ggcactcttt ttccagtacg ttgacgacct
    40201 gcatcatgcc tttgcagacg tccttatcaa taacgcctgc ctcgcaaagc aggttgagtc
    40261 tggtttccat atcagatgcc tttcgtttaa taactgggtt cctgttcccc actcaccacc
    40321 cgttgttgat gggcaagcag ggcgtgttta tctacaacag cacgcagcaa ttgttgcatt
    40381 gggtaatcat cgactcgcgt caggaccacg gcttcggtgg cctgtaaaaa tcgcggatcg
    40441 tctgttagcg gcgtcgcctc taatcccagc atggtcagtt cgttttccgc caccacgttc
    40501 cagatcaccg catcgacatc gcctttaaca atgcgttgta aactctcgtg ataagagaga
    40561 tcgactcgtt ccacatcact atcgccaaaa aagacatcgg tcatgatttt ctgatccgcc
    40621 gaacgactat cgagacctac tcgcttaaca tttccagact cacctttgcg gcatatcagt
    40681 tgatgttcac caacatacgt atgcgggcca agctcgaggg cgatgcacaa attgttttgg
    40741 ctgagataac tttccgccgc caggcgggaa actactgcca tgtcatagac accgttaagt
    40801 aaacactcca cgcgaatatc cgcgccgcgc atatgcgcat agtaaaaagg aatgccatca
    40861 aactgggctt tcaatccgct cgctaaacct tcgtacaagc gggtataggg caaaggcatc
    40921 gcacatacca cgttgttgat atccacatga ctcagcaatg ctttgttatc catctcgaca
    40981 agataactgc cattgcgccc acggcgttca atccgtatcg ctccgcttga ttccagcgtt
    41041 ttcaacgcgg cctgcgtcag gccaaccgat gaacggcatt cagttgccag ctcatctatg
    41101 gttttcagtc gattaccgca tttttcaccc aacaaataac gggccagtgt cgtgatgacg
    41161 acgccttctt ttttgataaa cgttcgacgc ataatatatt ttcaataaaa tgaatattta
    41221 tatcttcatt aaaatgaaga taagtggcga cgacgagatg tggaaacggc gaggcgcttc
    41281 acattttttc gggttgttgc gaaaagagga taaaaaaacc gggtttcccc ggtttcagag
    41341 tgatgacgaa gtgatttatt cgacaatgcg agcaaagggt aattcccact ggcgcttgcc
    41401 tgctcccctc accctaaccc tctccccaaa ggggcgaggg gaccgtccga gcgttttgat
    41461 attacggctt cgccacaaaa ccaatcgctt cgtacaccgc tttcagcgta cgggaagcgt
    41521 gtacactggc tttttctgcg ccgtctttca tcacctgttg caggaaggct tcatcgttgc
    41581 ggaaacggtg atagcgttcc tgcaattcag tcagcatacc ggaaacggca tcagccactt
    41641 cacctttcag atgaccatac atcttgcctt cgaactgttt ttccagttct gggatgctct
    41701 ggcccgttac cgcagaaagg atatccagca ggttggaaac gcccgctttg ttctgcacat
    41761 cgtagcgaac taccggcggc tcgtcggagt cagtgaccgc gcgtttgatt ttcttcacta
    41821 ccgatttcgg atcttccagc aggccgataa cgttattgcg gttatcgtca gacttggaca
    41881 tcttcttggt cggctccagc agcgacatta cgcgcgcgcc agatttcgga ataaacggct
    41941 ccggcacctt aaagatctcg ccatacagcg cgttgaaacg ctgggcgata tcgcgactca
    42001 gttccaggtg ctgtttctgg tcttcaccca ccggtaccag attagtttga tacagcagga
    42061 tgtccgcagc catcagcacc ggatagccaa acagaccagc gttgatgttc tcggcataac
    42121 gcgcagattt atctttaaac tgcgtcatgc gactcagttc gccgaagtag gtatagcagt
    42181 tcagtgccca gcctaactgc gcatgttctg gtacgtggga ctgaacaaaa atggtgcttt
    42241 tctcaggatc gataccacaa gccagataca aggccagcgt atccaacgtc gctttacgca
    42301 gcttctgtgc atcctggcgc acggtgatcg cgtgttggtc aacgatacag taaacgcaat
    42361 ggtagtcatc ctgcatgttt acccactgac gcagcgcacc catgtagtta ccaatggtca
    42421 attcacctga gggctgtgcg ccactaaaaa cgatgggctt agtcattttt cgattcctga
    42481 ttttcgctat gcggaagccc taatgcgggc agaaggtcat ttatagactg ataaattaca
    42541 tcaggctggc tgagatcgat agcctcgccg tagttgtatc cgtaggttaa gccaactgat
    42601 gggcaacctg ccgcttttgc cgcctgaata tcattccgtg agtcgccgac aaacagcatc
    42661 tgttgtgggg caattcccat ccgctcagcc accagtaaca gcgggtccgg atgcggtttt
    42721 ttgttttgca catcatcgcc gccgataacc acgctaaagt atttggcaat atctaaggct
    42781 tcgagcagcg gcgcgacgaa cggcgtcggt ttgttggtga ccaagcctag cggcaggcct
    42841 ttagcctgca acgcgcccaa cgtatcggca acgtgcggga acaaaaacgt cccctcttcg
    42901 gcaacctcgc catagtagcg atcgaacagt ttacgcagaa tacgtacctg ttcttctgcc
    42961 ggaatgtcgt catcaacggg cggtttcccc attgttttac gcagagtcgc acgttcctga
    43021 cgcgcccagg tcaatgcgcg ctccatcaga acatctgcgc cgttaccaat ccaggtaata
    43081 acgcgttctt cacctgcgac gggcaactcc agcgcataca gcgccatatc taccgcagca
    43141 gcaagaccag gagcactgtc gaccagcgta ccatcaagat caaaagcgac gccgcgaata
    43201 tcttcaaact tattcatgac ttacctttgc cagttcactg cgcatttcat caatgacttt
    43261 tttatagtct ggctggtcga agattgccga accggcgacg aacatatccg cgcccgccgc
    43321 agcgatttcg ccaatgttgt tcaccttcac gccaccgtcc acttccagac gaatgtcaaa
    43381 gccagactcg tcgatacggc gacgtacttc gcgcagttta tccagtgttt gaggaatgaa
    43441 agactgaccg ccgaaaccag ggttgacgga catcagcagg atcacatcca gcttatccat
    43501 cacgtaatcc agatagctca gaggtgtcgc cgggttaaat accagacccg ctttacagcc
    43561 attttcttta atcagttgca gcgtgcggtc aacatgctcg gaggcttctg gatgaaaggt
    43621 aatgatgctg gcaccagcgg cagcgaaatc aggcacaatg cgatcgacgg gtttcaccat
    43681 cagatgtacg tcgatagggg cggtaatgcc gtagttacgc aaggatttca gcaccattgg
    43741 cccaatcgtc agattgggaa catagtggtt atccatgacg tcaaaatgca cgacatcagc
    43801 gccagctgcc agggcttttg cggtatcttc acccaggcgg gcaaaatcag ccgacagaat
    43861 tgagggggca atcaaatact gtttcatccg cttctccttg agaattattt tttcgcgggt
    43921 gaaacgactc ctggtttgta caaagccagc agttcgtcca cctttttacg tgtgccgccg
    43981 ttgctgctta tactgcgtcg aactttgacg acatgcaatt ttgcgcgctg ataccactca
    44041 cgcgttaaca tcgtatcgtg attggagatc agcactggaa tatggcgctc aaccagacct
    44101 tcggcgatct ccgccagatg cgcttgttgt tcaagcgtaa aactgtttgt gtgatacgcc
    44161 gtaaagttgg cggtcgcaga cagcggtgca taaggcggat cgcaatagac gacggatgca
    44221 tcatctgcgc gctccatgct atcggcgtaa gactcacaat agaaaaaggc attctgcgct
    44281 ttttcagcga agtgatacaa ctctgcttcc gggaaatagg gttttttgta gcggccaaac
    44341 ggcacgttaa actcaccgcg cagattgtaa cgacacaggc cgttgtaacc gtagcggttc
    44401 aaatataaaa acagtaccgc ccgacggaac ggatcctggc ttttgttgaa ctcttcgcgg
    44461 aactgatagt aaacctcggc gcaatttgtt tcgggaacaa acagctcgcg tgcggcctgt
    44521 acgtactcat cagtacgcat cttcacaatg ttatagagac tgatcaggtc gctattgata
    44581 tcggcaagga tataacgaga aaagtcggtg ttgagaaaca ccgacccggc acctacaaaa
    44641 ggctcaacca gacattcgcc cttgggcaaa tgccgtttaa tatcatcaag caggggatac
    44701 ttgccccctg cccacttcaa aaaagcgcga tttttcttca tgccgattaa ctaattacac
    44761 cttctccggc tgtggagaaa gctccgacag catccagcgc ttcaggcagc tcccgcaacg
    44821 ataaaccatt gcgggagata accttgatta cttcagatcg gcctgtacct gacgcagcgg
    44881 tttcgcccac gggtttttgg cctgaacatc tgctggcaat gtagataccg cttttttcgc
    44941 ctcttctttc gaagcgtaca cgccagaaac caggacatac cacggctgac cattacgcgt
    45001 cgtttcatag acaacgtagt ttttcagatt ctctttcttc gcccaaccgt tcaggttgtc
    45061 gtagttagag gaactgctca gctgcagagt gtaatggctg gacggtgccg atttcaacga
    45121 accaacatta cctgcggtct tccctccagc ggctggtgtt gccgtggttt gcgccgggga
    45181 tgccgtctgt actggagccg tagtcgccgt ctcttttggt gctggcgtag aagtcgcagc
    45241 cggtgccttc gtgctcgcca caggggcagc tggttcagta cgcttcggcg tctgtgctac
    45301 cggcttcggc tccgttttca cggttgcttg cggttttttc ggttcaatca ccgcctgctg
    45361 acgaactggg cgcgtagtgg acggacgttc agcggtttgc gttttcgccg tgtcacgcga
    45421 tgcattgcca ttgcgaacag gcgcaaccgt tgcgggttca gtcggcaacg tggaattgac
    45481 cgccacattg ttcagctgtt gctgattttg tggctgggtc agcgcattgt tcaggtcacc
    45541 ctgcacttca acacgttgtt gaccatccgt tgccaccggg gtttgccctt gagtcggcgt
    45601 agaagagatc ggtggcagag aaacatcctg ctgagtattt tccgcagacg tggttcccgg
    45661 cgctggctgc acgccattcg cctgatcggt cgcattgcca gcaagatcaa tactcttctc
    45721 gccagacgcg gtttgatcgc tggaagtggt cgagggggct tttagcgcag aaccgatacc
    45781 gatgatcaac agcagtagaa ccagaatgcc gacgcccatc atcatatact gacgagaagc
    45841 gggtttactg gctgcttttt tgcgcttacg cggacgacgc tctacgcgct cttcatccac
    45901 ggtttcatct tcggattcgt caatttcttc ttcgatttcc ggttcctcat tgcgctcttt
    45961 ttgcgcacga gtcggacggc gatcgtcagt gtcatcaagt tcaatatcat caaaattgat
    46021 ctgcggttcg ccacgttcag tacgctcaga acgttcagaa gattgacgag aacgaccagt
    46081 acgacgatcg ctgggatcgg gtttcagctc gtcttctggt ttgaattcat ccatttaaca
    46141 ccccactaaa aggttaatgc ttaccacgtt gcaattaacc tgaagctaat agaccgcttg
    46201 ataagcggcc tgacctttct tgttgttacg ctgattgaca atcggcaatg gcgttaagaa
    46261 caagctcgtg cgaaacgccg ctgcgaactt cactcttacc aattgccaac ggaagaatta
    46321 agcgcatctc tcccgcaagg actttcttgt cacgcagcat atgcggtaaa tacgcctgcg
    46381 cggacatttc gcgcggccca ttgaccggta acccagcccg cgtgagcagg gttataatac
    46441 gctgcgtttc ggcagaacta aactgcccga gacgttccga cgtccgcgcc gccatcacca
    46501 tacccgcagc gaccgcttca ccatgtaacc aattgccata ccccatttca gcttcaatgg
    46561 catgaccaaa ggtgtgtccc agattcagta aagcacgtaa cccggtttcg cgctcgtcgg
    46621 cggcgacaac ttctgccttc agttcacaac aacggcgaat acagtacgcc attgccggac
    46681 cgtccagacg caacaacgca tccagattct cttccagcca gttaaaaaac gcaccgtcaa
    46741 gaataatgcc gtatttgatg acttctgcca gccccgacgc taactcacgc gggggaagcg
    46801 ttttcagaca gtcgagatcc accaccactg aagcaggctg gtagaacgcg ccaatcatgt
    46861 ttttaccgag gggatggttg accgcagttt tgccgccaac ggaggaatcg acctgcgaca
    46921 gtaacgtcgt cgggacttga atgaaacgaa caccgcgctg ataactcgcc gccgcgaagc
    46981 cggtcagatc gcccactacg ccgccgccaa gcgccaccag cgtagtatcg cgaccatgcg
    47041 gcttttgtaa caacgccgta aagacggtat cgagtacagc taggctttta tactgctcgc
    47101 cgtcagggag gataacgcta tcgacgttaa cacccgcctg ttcaagtacg ccgcggacct
    47161 tatcgagata cagaggagcc agggtttcgc tggtgaccaa catgacctgc tcgcccgatt
    47221 tcagcggtaa gaatgaagct ggttcattaa acaaaccaga tgcgatggta attgggtaac
    47281 tacgttcccc gagagtaacg acaatcctct ccataacgcg acatccacct taattactgt
    47341 acccgcagac gagtgtatat aaagccagaa ttagttgctt tccagcatgt gaataatctg
    47401 gtttgcaacc actttagcgc tttgatcatc agtacgaatg gtcacgtcgg caatctcttc
    47461 atacagcgga ttgcgttcat tggccaacgc ttccagaact tcacgcggcg gtgtttcaac
    47521 gtgcagcaac gggcgttttt tatcacgctg cgtgcgtgca agttgctttt cgatggtcgt
    47581 ttcaagataa acgacaacgc cacgagcgga aagacggtta cgcgtttcac gggatttcac
    47641 agagccgccg ccagtagcca gcacaatacc ctgtttctcg gtcaactcat tgatgacctt
    47701 ttcttcgcga tcgcggaagc cttcttcgcc ttctaaatcg aaaacccagc ccacatcagc
    47761 tccggttcgt ttctcaatct cttgatcgga atcgtaaaat tccatattga gttgttgagc
    47821 taactggcgc ccaatagtgc tttttccggc acccataggc ccaaccagaa agatattgcg
    47881 tttctctgcc attttttcgg tactactaag actattcgtt aatgataaac ccgcttcgct
    47941 cagagagcgc cgcaggacat gaactgaaac ctcataagat attgcgagag tcagactgaa
    48001 aattatctca atactccagc gggtttggca actgaataaa tcacccatcc ctctgcatat
    48061 ctggtcgctg caagcgcgct gccttgctac caccgctctg gcgataaatc accgggtaag
    48121 attagcgtaa aaaagacagc aaaatgccgc ctgaatgata aatcatcatc atggaacgca
    48181 ggcagcgaag agcaccaact ctcaaatcgg tactccttgt atgctaaata cctgcgcgcg
    48241 tcaaatagat gaaacacgtt cagcataaaa acattaccgc ttttacggct gtttactcac
    48301 tggaaaccag tcgtggcgtg ataaacacca ctaactcgcg tcgttcatct tcttttccgt
    48361 catgacgaaa taattgcccg aaccagggaa tgtcgccaag caacggtacg ctatcctgac
    48421 ccgatttatt tttacgggta aaaatgccgc ccagcgccaa cgtttctccg ctttttacct
    48481 cgacctgcgt ttcgatctcc tgcttatcaa tcgccagcac ttcgccatcg gcctgctgta
    48541 gcacctgccc cggaacgttc tggctgatgt gtaatttcag ccggatgcga cctttttgta
    48601 acaccgtggg cgtgacctcc atccccagga cggcctcttt aaattccacc gacgtcgcgc
    48661 cactttcccc gctggaaacc tgatatggaa tttcgctctc ctgtttaatg ctggcaggct
    48721 gaagatgtga ggccagcaga cgcggactgg cgataatatc cagctgctgt ttttgttcga
    48781 gcgcggaaag ctcaagatcc agcaagcgtc cgttgatgcg cccaatgtta aaaccgacat
    48841 gcgttgtcgc cgtcgctacg gagaggtcgc taccaagcgt ggtgacttgc ccaacgccac
    48901 cagcgtgttg cgcatcggcc agcgtccatt tcacgcctaa ctcacgcaaa cttttttcat
    48961 taatggtgac aatatgcgcc gacagctcaa cctgcccgac cggcagatcc atttgcgcta
    49021 cccactgttc aagcgcgctt aacgccgttt tgttatctcg tagcaaaagg cgattggtgc
    49081 gtttatcgac ggtcatactc cctttggcac tcagtagctt ctcccccgct ttcgccagtt
    49141 ctcccgcgtc ggcgtattgc agggttatac tgcgattttc cagcggcaga tttgcctgcg
    49201 cccgcgcctg ctccgcctcc tggcgggcga tattgtcatt ctgccaggca atggaatgca
    49261 ctgagagaat gttgccttcc tgccgcgtta tcagtccggc gcttttcact acagtttgta
    49321 gtgcctgctt ccagggaaca tctgttagat gtaacgacac cgtaccgctg acgtctggcg
    49381 acacgaccag gttcaacttc tcctgttcag ccagcgcctg caacacctga gctaccggaa
    49441 cgtcatccac catcagcgtc actttttgcg gctttgccgc ctgtacgccg ggtatcagca
    49501 tcaacagtag tgcggctatc cattgcttca tttgtatctc cttgccgttg ccacaaccat
    49561 tgtggcggtt cgcagtttgt cccggtaccc agcgttagta cgtctggcgt cagctgtaaa
    49621 attgtccagc cgttttccag cacatcgttt tgctgcaccc gtcgccattt cttttgcccg
    49681 tcttttatta caccgatgat gcgctcgcct cgccctacca tcccctgata gcgccactgg
    49741 ctaagttcgc taatccggca tagatcttcc ggcgatttaa aagggtcacg cataccggtt
    49801 aaaaggcaca atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag
    49861 ttgtagcgtg aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac
    49921 gttgcgctct gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc
    49981 caactcgcct ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa
    50041 atccagtggc gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg
    50101 atacagtgcg gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc
    50161 ttcatggtgt gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca
    50221 gcaaaactgg cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc
    50281 taaccttcct tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg
    50341 ctccccgctg attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta
    50401 acgcggtaat gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg
    50461 ttgtcagcca ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt
    50521 cgcgggtaaa ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct
    50581 ccagcaaacg tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca
    50641 aaactgcgtc tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga
    50701 cggccagcag cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc
    50761 gttgctgtcg ccagggcaaa aaattaattg gcgggttcat cagtacgcct tcccaagcgc
    50821 cagccccagc gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga
    50881 aacggcctcc caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac
    50941 ggataacgct gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca
    51001 gctatagcgc gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg
    51061 agaaggtaaa aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac
    51121 atgaacacgc aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc
    51181 agtcacgtta taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg
    51241 atccatatcc agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc
    51301 aagggacatc gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa
    51361 cataatgtga tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg
    51421 ctgcgcatca acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca
    51481 gcgttgcaaa aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg
    51541 ttgtaaatgc aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc
    51601 cgtcactctg acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt
    51661 tataaactgc ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt
    51721 tgatccttgc agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct
    51781 acatcgagcc acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga
    51841 tgcagattta cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc
    51901 cggttacgtt ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca
    51961 gccgcttcta cgagcatcac ggagttgacc cggtggggat cttccgtgcg gcaagtgtgg
    52021 cgctgttctc cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa
    52081 atttcttcct cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga
    52141 ttcgcattga acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt
    52201 accttggtta ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg
    52261 tcgaccaact gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca
    52321 cctttaatcc gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc
    52381 ggatgctgga tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga
    52441 ttaacgctaa ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg
    52501 tgcgccagga gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt
    52561 acaccaccat cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc
    52621 tggactacga catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg
    52681 agtcggcgtg ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc
    52741 cgctgctgcc tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg
    52801 acgggtcgac cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg
    52861 atactcagca gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa
    52921 tctgggttcg tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg
    52981 cgctggtgtc gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt
    53041 tcaatcagag caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca
    53101 aaccgttcct ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg
    53161 atgtgccaat ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac
    53221 caccgcagta tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg
    53281 tgatggtacg tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct
    53341 tcggcttccc ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct
    53401 tcaccccaat gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg
    53461 acccgtggtt tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac
    53521 cgaaagtagc ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga
    53581 acgtgctgga aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt
    53641 ctgtaccaat gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc
    53701 aggagtacgc accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga
    53761 acaccaatat ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt
    53821 tgcagcgtcg cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt
    53881 tctcgggtta cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc
    53941 gtaatctcgg tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag
    54001 gcggtgccaa gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg
    54061 tgccggagca gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca
    54121 ccgggcagtt agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc
    54181 cgacacaaca ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg
    54241 aattgttctg attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg
    54301 tttatgccgg atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata
    54361 ttgcaggaac cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca
    54421 ctcgcccctg ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt
    54481 cgttgaagtc agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg
    54541 gtagcggctc tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga
    54601 tattcatttt gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat
    54661 cgttcgcacc aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt
    54721 cacccggatc aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt
    54781 attcgcggat caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt
    54841 tggttggacg cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc
    54901 tctcgacggt aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt
    54961 gtaatgattt gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg
    55021 gacacgcttt cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat
    55081 cgtcctcaaa tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt
    55141 ccgccttcag agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag
    55201 ttaagctcca cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg
    55261 accacgcctg acagactaag taagatgggg aaagcatgag caccattgtg atttttttag
    55321 ctgctttgct ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc
    55381 gtcagctgcc ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag
    55441 aacgtagcgc cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc
    55501 caacgggagc tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca
    55561 tgatgctgac acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc
    55621 gttactacct cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg
    55681 atgagaatac cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg
    55741 gtcatacgct gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt
    55801 ccacgcaggc gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca
    55861 aagaaacgca tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga
    55921 tcgtcgcctc cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt
    55981 ggctggcagg cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc
    56041 cggcaaaatc ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat
    56101 tgtttggcga aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg
    56161 tctatcccgc acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg
    56221 atatctatct cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag
    56281 taaaaaactt tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc
    56341 tggtgctgtt tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct
    56401 catggatgaa aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg
    56461 gcgtgcgggt aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt
    56521 ccggaacctg gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca
    56581 tctggaatga cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg
    56641 cattgactga agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca
    56701 gtgcctcatt gcgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg
    56761 acattgtact gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa
    56821 atgcgctggt caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg
    56881 ccgggaagct cgatggcgtg aatgtgttat tacgcccggt gagtgcggaa tcgctggata
    56941 acctggtggc aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat
    57001 cactaaacag tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg
    57061 ttgatcagcc ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg
    57121 ctttccagaa actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca
    57181 caaaaatctt cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc
    57241 gttccggcct gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg
    57301 ccatttacaa tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg
    57361 agattcaggc ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc
    57421 ttatcgaata acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact
    57481 ctggagattt ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg
    57541 gatatggacg gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg
    57601 cctgaaacat ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc
    57661 cagcaatatc acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag
    57721 caactgggtc tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt
    57781 gaagatacca ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc
    57841 accaatgctc atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac
    57901 cttgatttat tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg
    57961 catgcggtgg ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc
    58021 gaagcgattc tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat
    58081 cctgattccg ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc
    58141 cgcctgatcc cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt
    58201 tcgactggat aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga
    58261 aatgattgaa ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt
    58321 cgagctgaat gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa
    58381 ggcgattact gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc
    58441 ggaaagtgta gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat
    58501 gccgcacccg gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca
    58561 cggcgacagt gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt
    58621 acatcgctat ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac
    58681 cctgcaacag atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga
    58741 actgctggtt gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt
    58801 acagctgcag ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca
    58861 aatgcgcggt gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct
    58921 ggtcggcaat ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg
    58981 cgtagttggt ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc
    59041 tgaacagctg ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc
    59101 aggcggtatg ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca
    59161 cctggcgacg ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga
    59221 agtgttgtgg cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga
    59281 gttcaaatgc acctgctcgc gtgaatgttg cgccgatgcg ctgaaaacgc tgcctgatga
    59341 agaagttgat agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg
    59401 taaccactat ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc
    59461 agatccgtaa gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac
    59521 ttaatggcct gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg
    59581 tcagatacag cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt
    59641 ttcaccatct accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt
    59701 cgcagctgtg agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc
    59761 gtcccattct tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc
    59821 aaacaggatc cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc
    59881 gtctttttca atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt
    59941 cgcatccggt ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga
    60001 tacctgttcc tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt
    60061 cgggtctttc ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg
    60121 accgctctgt tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata
    60181 acgcgccata tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag
    60241 caccagtatc accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag
    60301 gggattttgc ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa
    60361 caccagctcc gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg
    60421 cagccataac ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt
    60481 aatcaggcaa cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat
    60541 agccattaag atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg
    60601 gcgagaaatc gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa
    60661 tgatactaac ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat
    60721 taaacgtgac tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag
    60781 atacataaac cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt
    60841 aaacaactca ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac
    60901 cagtaatata agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc
    60961 attgcggata cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag
    61021 atagcagcca aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg
    61081 ccacttcagc caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca
    61141 aaggcgcttc tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt
    61201 cgcaggcacg ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag
    61261 gtagcaaacc agcccctgaa gtaatccggt agcaatcatc ccccagcgtg ttgccggtga
    61321 aagttcaacg ttatccatgt tggttatcca gaatcaaaag gtgggttaat tatcgcatcc
    61381 gggcagtagt attttgcttt tttcagaaaa taatcaaaaa aagttagcgt ggtgaatcga
    61441 tactttaccg gttgaatttg catcaatttc attcaggaat gcgattccac tcacaatatt
    61501 cccgccatat aaaccaagat ttaacctttt gagaacattt tccacaccta aaatgctatt
    61561 tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga gttttttgtc aaatatgaat
    61621 ttctccagat acgtaaatct atgagccttg tcgcggttaa cacccccaaa aagactttac
    61681 tattcaggca atacatattg gctaaggagc agtgaaatgc gcgttaacaa tggtttgccc
    61741 ccgcaagaac tcgaggctta tggtatcagt gacgtacatg atatcgttta caacccaagc
    61801 tacgacctgc tgtatcagga agagctcgat ccgagcctga caggttatga gcgcggggtg
    61861 ttaactaatc tgggtgccgt tgccgtcgat accgggatct tcaccggtcg ttcaccaaaa
    61921 gataagtata tcgtccgtga cgataccact cgcgatactt tctggtgggc agacaaaggc
    61981 aaaggtaaga acgacaacaa acctctctct ccggaaacct ggcagcatct gaaaggcctg
    62041 gtgaccaggc agctttccgg caaacgtctg ttcgttgtcg acgctttctg tggtgcgaac
    62101 ccggatactc gtctttccgt ccgtttcatc accgaagtgg cctggcaggc gcattttgtc
    62161 aaaaacatgt ttattcgccc gagcgatgaa gagctggcag gtttcaaacc agactttatc
    62221 gttatgaacg gcgcgaagtg cactaacccg cagtggaaag aacagggtct gaactccgaa
    62281 aacttcgtgg cgtttaacct gaccgagcgc atgcagctga ttggcggcac ctggtacggc
    62341 ggcgaaatga agaaagggat gttctcgatg atgaactacc tgctgccgct gaaaggtatc
    62401 gcttctatgc actgctccgc caacgttggc gagaaaggcg atgttgcggt gttcttcggc
    62461 ctttccggca ccggtaaaac caccctttcc accgacccga aacgccgcct gattggcgat
    62521 gacgaacacg gctgggacga tgacggcgtg tttaatttcg aaggcggctg ctacgcaaaa
    62581 actatcaagc tgtcgaaaga agcggaacct gaaatctaca acgctatccg tcgtgatgcg
    62641 ctgctggaaa acgtcaccgt gcgtgaagat ggcactatcg actttgatga tggttcaaaa
    62701 accgagaaca cccgcgtttc ttatccaatc tatcacatcg ataacatcgt taagccggtt
    62761 tccaaagcgg gccacgcgac taaggttatc ttcctgactg ctgatgcttt cggcgtgttg
    62821 ccgccggttt ctcgcctgac tgccgatcaa acccagtatc acttcctctc tggcttcacc
    62881 gccaaactgg ccggtactga gcgtggcatc accgaaccga cgccaacctt ctccgcttgc
    62941 ttcggcgcgg cgttcctgtc gctgcacccg actcagtacg cagaagtgct ggtgaaacgt
    63001 atgcaggcgg cgggcgcgca ggcttatctg gttaacactg gctggaacgg cactggcaaa
    63061 cgtatctcga ttaaagatac ccgcgccatt atcgacgcca tcctcaacgg ttcgctggat
    63121 aatgcagaaa ccttcactct gccgatgttt aacctggcga tcccaaccga actgccgggc
    63181 gtagacacga agattctcga tccgcgtaac acctacgctt ctccggaaca gtggcaggaa
    63241 aaagccgaaa ccctggcgaa actgtttatc gacaacttcg ataaatacac cgacacccct
    63301 gcgggtgccg cgctggtagc ggctggtccg aaactgtaat gatttgaagc tggagaatat
    63361 ctatccagta tcttatagaa agcaaaacgg gaggcacctt cgcctcccgt ttatttaccc
    63421 ttcttttgtc gtgccctgcg cccgcgttac cggcactggc agccaggcgc gaatggaaag
    63481 cccgccccgc tcgctggtgc caagctccag catcccgtta tggttatcca cgatacgctg
    63541 cacaattgcc agccctaatc ccgtgccgct aatggtgcgc gcactgtcgc cgcggacaaa
    63601 cggctggaac aggtgcttac gttgttccgg cgcaattccc ggaccgtcat cttccacctg
    63661 gaaccaggcg cgattcggct ccgttccgct gctgactttg atccagccat tgccataacg
    63721 ggcggcgttg accaccatat tcgccaccgc gcgtttgatc gacagcgggt gcattttcac
    63781 ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc tcatagccac tttcggcagc
    63841 aatcacctca ccgagcactg cattaagatc cgccatttcc atcggcatct cctgcccggt
    63901 gcgcaggtag tcgataaact gctcaatgat ggcgttgcac tcttcgatat ctttattgat
    63961 cgattctgcc agatagccat cctgctcgct catcatctca gtcgccaggc gaatacgcgt
    64021 cagcggcgtg cgcaagtcgt gacttacccc cgccatcagc agcgtgcggt catccgccag
    64081 ttgcttaaca ccagccgcca tatggttaaa ggcacgggta acggaacgca cctccgaagc
    64141 gccatattca cgcagcggcg gcggaataat ccctttacca acctgcaagg ctgcgtgttc
    64201 gagatcgacc aacggtcggt tctggatacg aataaacagc cacgccccgc ctatcgccaa
    64261 tagcataatc gccagcgtat agcggaacag cggagagaaa tcgccctgat gaatttcggt
    64321 cagcggcacg cgtacccaga tattgggcga cagccaggtt ttcagccaga cgacaggcga
    64381 acttttgttg acctcaacgc gcacttccgt cgggccgccc agttgctgtg ccatctgatg
    64441 gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc tcttcggcag cctcgttgga
    64501 gtagagagag atccccagct cacggtagat ctcccgacgg aaagcgggag gcacaaccaa
    64561 ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac atacgcactt cgtacgcgag
    64621 gactttatta aactgctgga ggctcggcaa aatcgcgaag ttcagcacca ccagataagt
    64681 cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat aacgtacggg caaatgaact
    64741 tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt ccggtacaaa gacgtagccc
    64801 aggccccaaa cggtctgaat gtaacgcgga tgcgctggat cttcttctac catgcggcgc
    64861 agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg aatattcacg accacgggca
    64921 aggttcatca gcttatcgcg ggagagcggc tcacgcggat ggctgaccag tgccttcagt
    64981 accgcaaact caccgctggt gagcggcatc ggctcatctt cgcggaacat ttcgcgcgta
    65041 ccgaggttaa gtttgaactt accgaaagca attaccgcct cttcctgtga cggtgcgcct
    65101 ggcagttcgt tcgcctgacg acgcagcacc gcacgaatac gggccagcag ttcacgcggg
    65161 ttaaacggtt ttggaatgta gtcgtcagcg ccaatctcca ggcctacgat acggtccact
    65221 tcttcccctt tcgccgtcac cataatgatc ggcatcgggt tgctctgact acgaagacgt
    65281 cggcaaatcg acaagccatc ttcaccaggt aacattaaat ccagtaccat aagatggaaa
    65341 gattcacgag tcagcaggcg atccatctgt tctgcattag cgacgcttcg aacctggaag
    65401 ccttgttcgg tgagataacg ttccagcagc gcacgcaggc gcatgtcgtc atcgaccacc
    65461 agaatcttgt agttctcttg cattgtttgt actcccaaag gttcgcaaca atttgtaagc
    65521 gtgtattctt aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa tttcagccta
    65581 aattgttaca aagcatatta aacagcagct taagtataca atttattcgg cgaaacatta
    65641 ttgattctgt tgatatgatc acgttatacc caatgtgcgc attatcaaac agacaaaggg
    65701 aatcaacgag atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac tcaaacaaga
    65761 gcttaattat ctctggcgtg aagaacgccc ggaggtcaca aaaaaggtga cctgggccgc
    65821 aagtctgggc gaccgcagcg aaaatgctga ctatcagtat aataaaaagc gtctgcgtga
    65881 aatcgaccgt cgcgtgcgct atctcactaa atgcctggaa aatctcaaaa tcgtcgatta
    65941 ctcccctcag caggaaggca aagtcttttt tggcgcgtgg gtggagattg aaaacgacga
    66001 tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa atttttggcc gtaaagatta
    66061 catctctatc gattccccga tggcccgcgc attgctgaaa aaagaagtcg gcgatctggc
    66121 ggtggtgaat acccctgccg gggaagcgaa ctggtatgtt aatgctatcg agtacgtgaa
    66181 accgtaagga agagtcttaa cctcctgccg atggctggca tttttgccag ccagtccgta
    66241 taactatccc ctgatttttg atccgaaaag atgaactcaa accatgatga atgattcgtt
    66301 ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa caggttgacg ctgccgttcg
    66361 cctgcttgac gaagggaata ccgtgccgtt tatcgcacgt tatcgtaagg aaatcaccgg
    66421 cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt ctgagctatc tgcgcgagct
    66481 ggaagagaga cgtcaggcga tcctcaaatc catttccgag caaggcaaac tcaccgatga
    66541 tctggcgaag gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc tctacctgcc
    66601 ctacaaacct aaacgccgca cccgcgggca aatcgccatt gaagcagggc ttgagccgtt
    66661 ggctgacctg ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg cacaatatgt
    66721 tgatgccgat aaaggcgtgg cagataccaa agccgcgctg gatggcgcgc gctatatcct
    66781 gatggaacgg tttgccgaag atgccgcgct gctggcgaaa gtgcgtgatt atctgtggaa
    66841 gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa gaggaagggg cgaaattccg
    66901 cgactatttc gatcatcacg aaccgttgtc cacggtgcct tctcaccgcg cgctggcgat
    66961 gttccgtggg cgtaacgaag gcgtactcca gctttcgctg aatgccgatc cgcagttcga
    67021 tgagccgccc aaagagagct attgcgagca aatcatcatg gatcaccttg gcctgcgcct
    67081 gaacaatgcc ccggcggata gctggcgcaa aagcgtagtg agctggacct ggcgcatcaa
    67141 ggtgctgatg catctggaaa ccgaactgat gggtaccgtg cgcgaacgcg cggaagatga
    67201 agcaatcaac gtctttgccc gtaacctgca cgatctgctg atggcggccc ctgccggact
    67261 gcgtgcaacg atgggcctcg atccgggtct gcgtaccggg gtaaaagtgg cggtggtcga
    67321 tgccactggc aaactggtgg cgaccgatac catttacccg cacaccggac aagccgcaaa
    67381 agcagcgatg accgttgctg ccttgtgtga aaaacataac gttgaactgg tggcgatcgg
    67441 caacggtaca gcttcccgcg aaactgaacg tttctatctc gacgtgcaga agcagttccc
    67501 gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc gcgtcggttt actcagcttc
    67561 cgagctggca gcgcaggagt tcccggatct cgacgtttcg ctgcgtggtg cggtatctat
    67621 cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa atcgatccga aatctatcgg
    67681 cgtaggtcag tatcagcatg acgtcagcca gacgcaactg gcccgcaaac tggatgcagt
    67741 agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac accgcttctg ttccgctatt
    67801 aacccgcgtg gcgggcctga cgcgcatgat ggcgcaaaac atcgtggcct ggcgcgatga
    67861 gaacggccag ttccagaacc gtcagcaact gctgaaagtc agccgtctgg ggccgaaagc
    67921 cttcgagcag tgcgcgggct tcctgcgcat taaccacggt gataacccgc tggacgcctc
    67981 taccgttcat ccggaagcct atccggtggt ggaacgcatt ctggcagcaa cacagcaggc
    68041 actgaaagat ctgatgggta acagcagcga actgcgtaac ctgaaagcgt ctgactttac
    68101 tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc aaagagctgg aaaaaccggg
    68161 tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg agacaatgaa
    68221 cgacctgcaa ccgggtatga tcctcgaagg cgcagtgacc aacgtcacca actttggcgc
    68281 gtttgtcgat attggcgtgc atcaggacgg cctggttcac atctcttcat tatcgaacaa
    68341 gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac attgtgaagg tgaaagtgct
    68401 ggaagtggat cttcagcgta aacgtatcgc cctgactatg cgtctggacg agcagcctgg
    68461 cgaaaccaac tctcgtcgcg gcggcggtaa tgaacgcccg caaaacaacc gcccggcagc
    68521 caaaccacgc ggtcgtgaag cgcagcctgc cggtaatagc gcgatgatgg atgcgctggc
    68581 ggcagcgatg ggtaaaaaac gttaaacgcc cggactggcc cctaaacctg cgcgataagg
    68641 cgttcacgcc gcatctggca acgaacgctt tgcctgacat aaaagtgccg gagaatatct
    68701 ccggcacttt tattccacag ccaaactcac aatatactcc gttaatattt atcacttcat
    68761 taacaactga aaccttaatt aaacattagc cagtcctggt aactcactat tcgaattata
    68821 ttttcgctgc gatataacct tgagccacat caacattgag tcagattatt attcaaacca
    68881 acattcgcac acattttaag tattgctgat agaaaccatt ctcattatca ttgtgttgtt
    68941 gattatttaa tctctccttt gttggcaaat catctggtct catgtcgcgg tcaaacgccc
    69001 catgaggtag ttatccagtt aatgagaaac aagtaggcac ctatgcaata cactccagat
    69061 actgcgtgga aaatcactgg cttttcccgt gaaatcagcc cggcatatcg ccaaaaactg
    69121 ctttctctag gcatgttacc tggctcctct tttgatgtgg tgcgcgtcgc tccactcggc
    69181 gaccccattc atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa agatctggcc
    69241 ttattagaag tggaagcggt ttcctgttaa tacggtgata acaacaatga aaaaattaac
    69301 cattggctta attggtaatc caaattctgg caagacaacc ttatttaacc agctcactgg
    69361 cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag aagggcaatt
    69421 ctccaccacc gatcatcagg tcacgctggt ggacctgccc ggcacctatt ctctgaccac
    69481 catctcatcg cagacctcgc tcgatgagca aatcgcctgt cactacattt tgagtggcga
    69541 cgccgacctg ctgattaacg tggtggatgc gtctaacctt gagcgtaacc tgtacctgac
    69601 gctgcaactg ctggaactcg gcattccctg catcgtggcg ctgaacatgc tcgacattgc
    69661 cgagaagcaa aatattcgta ttgaaattga tgctctatcg gcacgtcttg gctgcccggt
    69721 gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg cgattgatcg
    69781 ctataaagct aacgagaatg tggaactggt gcattacgca cagccgctac tcaacgaagc
    69841 agattcactg gcaaaagtga tgccttccga catcccgctg aaacaacgtc gctggctggg
    69901 cctgcaaatg ctggaaggcg atatctacag ccgcgcctac gccggtgaag cgtcgcagca
    69961 tctggatgcc accctcgccc gtctgcgtaa tgaaatggat gatccggcgc tgcacattgc
    70021 cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg gtaagcaaca ccctgacggc
    70081 agaacccagc cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt tcctcggtct
    70141 gccgattttc ctctttgtga tgtacctgat gttcctgctg gctatcaaca tcggcggggc
    70201 gttacagccg ctgtttaacg tcggctccgt ggcgctattt gtgcatggta ttcaatggat
    70261 tggctacacg ctccacttcc cggactggct gactatcttc ctcgcccagg gcctgggtgg
    70321 cggtattaac accgtgctgc cactggtgcc gcagatcggc atgatgtacc tgttcctctc
    70381 cttccttgag gactccggct atatggcgcg tgcagcgttt gtgatggacc gcctgatgca
    70441 ggcgctgggc ttgccgggga aatcctttgt gccgctgatc gtcggtttcg gttgtaacgt
    70501 accgtcggta atgggcgcac gtacgcttga tgcaccgcgt gaacgtctga tgaccatcat
    70561 gatggcaccg tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat tcgcggctgc
    70621 cttcttcggg cagaacggtg ccctggcggt tttctcgctg tatatgctcg gtatcgtgat
    70681 ggcggtgctg actggcctga tgctcaagta caccatcatg cgcggtgaag cgacgccgtt
    70741 tgtcatggag ctgccggtct accatgtacc acacgttaaa agcctgatta tccagacctg
    70801 gcagcgtctg aagggcttcg ttctgcgtgc tggtaaagtg attatcatcg tcagcatttt
    70861 cctgagcgct ttcaacagct tctcgttgag cgggaaaatc gtcgataaca tcaacgactc
    70921 ggcgctggct tccgtcagcc gggtgataac ccctgtcttc aagccgattg gcgtacatga
    70981 agataactgg caggcaacgg ttggcctgtt tacgggcgca atggcaaaag aagtggtggt
    71041 gggtacgctc aacaccctct acaccgcaga aaatattcag gacgaagagt tcaatccggc
    71101 ggaattcaac ctcggtgaag agctgttcag tgcggtagat gaaacctggc agagcctgaa
    71161 agacaccttc agccttagcg tactgatgaa ccccattgaa gctagcaaag gcaacggtga
    71221 aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag ctgccgcata
    71281 cagctacctg attttcgtcc tgctgtatgt accatgtatc tcggtgatgg gagccatcgc
    71341 ccgtgaatca agccgtggct ggatgggctt ctccatcctg tgggggctga atatcgctta
    71401 ctcactggca acattgttct atcaggtcgc cagctacagt cagcacccaa cttacagcct
    71461 ggtgtgcatt ctggcggtaa tcctgtttaa catcgtagct atcggtctgc tgcgccgcgc
    71521 gcgtagccgg gtggatatcg aactgctggc aacccgcaag tcggtaagca gttgctgcgc
    71581 agccagcacc accggtgatt gccattaatg gcttcactta ttcaggtacg tgatttgctg
    71641 gcgttacggg gccgtatgga agcgacccag ataagccaga cattgaacac tccgcagcca
    71701 atgattaacg ccatgctgca acagctggaa agcatgggca aagccgtgcg gattcaggaa
    71761 gaacctgacg gctgcctctc tggcagttgt aaaagctgcc cggaaggcaa agcctgtctg
    71821 cgcgagtggt gggcgctgcg ttaaccatac tccatcgcct gttttgaaaa gcccggtatg
    71881 cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa aatgcaattc ttgcagcgca
    71941 acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact cgctaatgca cagatgtgga
    72001 gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg aacgccatga gtaaaaagca
    72061 gagttccacc ccacatgatg cgctgttcaa actcttttta cgccaaccgg agacggcacg
    72121 cgattttctt gcgtttcatt taccggcacc cattcacgcg ctctgtgata tgaaaaccct
    72181 caagctggag tcgaggagct ttattgatga cgatctgcgt gaaagctatt ccgatgtgct
    72241 gtggtcggtg aaaacggaac aaggaccagg atacatctat tgtctgattg aacatcaaag
    72301 cacctcaaac aaactgatcg catttcgcat gatgcgttac gctattgccg caatgcaaaa
    72361 tcaccttgat gctggataca aaacgttgcc gatggtggtg ccattgttgt tttaccacgg
    72421 tattgaaagc ccctatccct attcgctgtg ttggctggat tgtttcgccg atcccaaact
    72481 ggcaaggcag ctttatgcct ccgcatttcc gctgattgat gtcaccgtca tgcctgatga
    72541 tgaaatcatg cagcaccgac gtatggcgct gctggagtta attcaaaaac atattcgtca
    72601 acgcgatctg atggggctgg tagagcaaat ggcctgctta ttaagtagtg gatacgctaa
    72661 tgacagacaa atcaaagggc tgtttaatta catactgcaa actggcgacg ctgtacgttt
    72721 taacgatttt atcgacggcg ttgccgaacg ttcaccgaaa cacaaggaga gtttaatgac
    72781 tattgcggaa agattgcggc aggaggggga acaatccaaa gccctgcata tagccaaaat
    72841 aatgcttgaa tccggagtcc ctcttgcaga catcatgcga tttaccgggc tgtcagaaga
    72901 agagttggct gcggcg
//
LOCUS       DAJTRB010000004        70700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTRB010000004 DAJTRB010000000
VERSION     DAJTRB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.004"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..794
                     /locus_tag="OO330_000311"
     CDS             <1..794
                     /locus_tag="OO330_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5881327.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="OO330_000312"
     CDS             805..1896
                     /locus_tag="OO330_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5881328.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MHAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO330_000313"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO330_000313"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5881329.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="OO330_000314"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="OO330_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5881330.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="OO330_000315"
     CDS             4858..5253
                     /locus_tag="OO330_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5881331.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="OO330_000316"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="OO330_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5881332.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="OO330_000317"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="OO330_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5881333.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="OO330_000318"
     CDS             complement(6562..7602)
                     /locus_tag="OO330_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5881334.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="OO330_000319"
     CDS             complement(7675..8310)
                     /locus_tag="OO330_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5881335.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="OO330_000320"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="OO330_000320"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5881336.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="OO330_000321"
     CDS             complement(8936..9379)
                     /locus_tag="OO330_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5881337.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO330_000322"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO330_000322"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5881338.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="OO330_000323"
     CDS             complement(9719..10222)
                     /locus_tag="OO330_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5881339.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="OO330_000324"
     CDS             complement(10216..10740)
                     /locus_tag="OO330_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5881340.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="OO330_000325"
     CDS             10949..11944
                     /locus_tag="OO330_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5881341.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="OO330_000326"
     CDS             11953..12831
                     /locus_tag="OO330_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5881342.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="OO330_000327"
     CDS             12912..13919
                     /locus_tag="OO330_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5881343.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO330_000328"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO330_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5881344.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO330_000329"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO330_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5881345.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO330_000330"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO330_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5881346.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO330_000331"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO330_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5881347.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO330_000332"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO330_000332"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5881348.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO330_000333"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO330_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5881349.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO330_000334"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO330_000334"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5881350.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO330_000335"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO330_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5881351.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO330_000336"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO330_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5881352.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO330_000337"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO330_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5881353.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYGQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO330_000338"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO330_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5881354.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="OO330_000339"
     tRNA            complement(27653..27729)
                     /locus_tag="OO330_000339"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="OO330_000340"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="OO330_000340"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5881355.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="OO330_000341"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="OO330_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="OO330_000342"
     tRNA            complement(31513..31599)
                     /locus_tag="OO330_000342"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OO330_000343"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OO330_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5881356.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OO330_000344"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OO330_000344"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5881357.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OO330_000345"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OO330_000345"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5881358.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OO330_000346"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OO330_000346"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5881359.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OO330_000347"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OO330_000347"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5881360.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="OO330_000348"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="OO330_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5881361.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OO330_000349"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OO330_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5881362.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="OO330_000350"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="OO330_000350"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5881363.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVVSYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OO330_000351"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OO330_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5881364.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="OO330_000352"
     CDS             complement(41211..42176)
                     /locus_tag="OO330_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5881365.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OO330_000353"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OO330_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5881366.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OO330_000354"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OO330_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5881367.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="OO330_000355"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="OO330_000355"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5881368.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="OO330_000356"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="OO330_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5881369.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OO330_000357"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OO330_000357"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5881370.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OO330_000358"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OO330_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5881371.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OO330_000359"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OO330_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5881372.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OO330_000360"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OO330_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5881373.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OO330_000361"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OO330_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5881374.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OO330_000362"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OO330_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5881375.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFTGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OO330_000363"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OO330_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5881376.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="OO330_000364"
     CDS             49714..50679
                     /locus_tag="OO330_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5881377.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="OO330_000365"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="OO330_000365"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5881378.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="OO330_000366"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="OO330_000366"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5881379.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="OO330_000367"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="OO330_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5881380.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="OO330_000368"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="OO330_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5881381.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="OO330_000369"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="OO330_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5881382.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="OO330_000370"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="OO330_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5881383.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="OO330_000371"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="OO330_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5881384.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="OO330_000372"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="OO330_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5881385.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="OO330_000373"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="OO330_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5881386.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="OO330_000374"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="OO330_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5881387.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58510
                     /gene="yrbL"
                     /locus_tag="OO330_000375"
     CDS             57878..58510
                     /gene="yrbL"
                     /locus_tag="OO330_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5881388.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58507..59234)
                     /gene="mtgA"
                     /locus_tag="OO330_000376"
                     /pseudo
     CDS             complement(58507..59234)
                     /gene="mtgA"
                     /locus_tag="OO330_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59231..59884)
                     /gene="elbB"
                     /locus_tag="OO330_000377"
     CDS             complement(59231..59884)
                     /gene="elbB"
                     /locus_tag="OO330_000377"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5881389.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60114..62450)
                     /gene="arcB"
                     /locus_tag="OO330_000378"
     CDS             complement(60114..62450)
                     /gene="arcB"
                     /locus_tag="OO330_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5881390.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62546..63475)
                     /locus_tag="OO330_000379"
     CDS             complement(62546..63475)
                     /locus_tag="OO330_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5881391.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDVVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGIVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64150..68610
                     /gene="gltB"
                     /locus_tag="OO330_000380"
     CDS             64150..68610
                     /gene="gltB"
                     /locus_tag="OO330_000380"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5881392.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68623..70041
                     /gene="gltD"
                     /locus_tag="OO330_000381"
     CDS             68623..70041
                     /gene="gltD"
                     /locus_tag="OO330_000381"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5881393.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70225..70629
                     /locus_tag="OO330_000382"
                     /pseudo
     CDS             70225..70629
                     /locus_tag="OO330_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
BASE COUNT        17312 a        19175 c        17553 g        16660 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc acgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccaccattac cgtcatggtg cggtaaaaga agacgttacg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgta
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa cgccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctactgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtagtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgcattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacacgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ctggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgccca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caggctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagtgagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg tatcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatcg ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgatgaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggtaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt aattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaagtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggcgatgg tggcgataaa gagatccgcc gtgagttaaa
    58021 gtactacgcg catcttggtc gccggttaaa agactggagt ggaataccgc gctatcacgg
    58081 taccgttgag acggattgcg gaaccggtta tgtctacgat gtgattgccg attttgacgg
    58141 caagccttcc attacgctga ccgaatttgc tgaacaatgc cgttacgaag aagacattgc
    58201 acaactgcgc cagctactga aacagctgaa gcgttatttg caggataacc gtatcgtgac
    58261 gatgtcgctg aagccgcaga atatcctctg tcaccgcatc agtgagtcag aggttatccc
    58321 ggtcgtctgc gacaacattg gtgaaagcac attgattcca ctggcgacct ggtcgaagtg
    58381 gtgttgtctg cgtaagcaag agcggttatg gaaacgattt atcgcccaac cggcgctggc
    58441 tattgccctg cagaaagatc tgcaacctcg tgagagcaaa acgctggccc tcacttcgcg
    58501 cgaagcttaa tccagctggt gttgttgcat aaacggctca ccgcctaact gatacatctg
    58561 ccgtaaaatc cacgcctgac ggctacgcac gtagcccgat ggcgaggaga ctttgaaacg
    58621 aagtggatta ggtaatacag ctgccagtaa tgcagcttcc gaccgggtaa gtttgctcgc
    58681 gggtttgtgg aaataacgtt gtgccgcagc ttcgacgcca aacacaccgt cgccaaattc
    58741 ggcgatattc aggtaaaccg tcaggatacg ctttttgctc cagactgttt ctatccccag
    58801 cgttaatcca gcttccagcc cttttcgtac ccagctacgc ccatcccata aaaagagatt
    58861 ttggccgtct gctgagaaat cgttgaagca ccgcgaatac agttttcatt gcgctcgttg
    58921 tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc agtgctcagg aaatttctga
    58981 tcttctgcgg caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag
    59041 tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc
    59101 attaccgctg agaagggaac aggcgcaacg ctaaacaacg cgatgccccc gccccagaaa
    59161 acggcgagga caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag
    59221 cggcttttac tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg
    59281 ccgcttctgc aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt
    59341 catcaaccac gatatcatcg acaggacacg gcacatgctc cgcgcccatc tcttccagca
    59401 cttctgcggt atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg
    59461 gcagcatcgc cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt
    59521 gtgccagcgc ctttaattca cggtcaacgg tgcattcgct accaagactg gcaaaattgc
    59581 ttaaattctt cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat
    59641 cggcctgggc cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacat
    59701 tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat
    59761 ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg
    59821 cttcatgaat ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct
    59881 tcattgtgat catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt
    59941 acgcatcaca catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg
    60001 cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag
    60061 ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt
    60121 ttagtggctt ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc
    60181 atctcttcaa tccattcacc gacgttatct tcccaggcgg gaaggtcagg agactgaatt
    60241 tgctgaccca gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta
    60301 tgtccttcct caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg
    60361 acatagcccg gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct
    60421 acaagttcga gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc
    60481 tcttctgtcg tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc
    60541 gcggttagcg ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg
    60601 tactcttgtt tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg
    60661 cgcggataac gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga
    60721 atatccagca acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg
    60781 gtcatggcga catcaacgct gttacctaat ttttccagca cagaacgcgc gacaatcacg
    60841 ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca
    60901 tcaaacgcat catcgacctc ttctgccacc gacggtgcgt ggatcgtcaa cgtaaaggtt
    60961 gaacctttgc cctgttcact ggtaaccgta atatcgccgc ccatattttt cgccagacga
    61021 cgagaaacgg ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc
    61081 acctggtaat acatggcgaa gattttatcc agctcatcct gcggaatgcc gataccagaa
    61141 tcttccactt caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg
    61201 ccttgctggg tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc
    61261 gtcccgtcgg taatgacctg atgcggtaat ggcagcgtag gctccaggtt aaagcgcaat
    61321 cctttttgtt gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa
    61381 tcaaccggct gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata
    61441 tcgttaaaga tattccccag cgtgacggcc gaaacatgga tagttttgag atatttttcc
    61501 tgctcggcgg tgagttcggt atccagcaga atgcggctca ggccgacgat accgttcagc
    61561 ggtgtacgca attcgtgact gatggtggaa ataaacgtcg ttttgtcgcg gctggcgcgt
    61621 tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg
    61681 tgacgtttac ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc
    61741 ccgtccgggt aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt
    61801 tcatcggttt caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg
    61861 tgaaccagtt gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa
    61921 aactctttat cttcgttacg ataaaaaacc aagtcgggtg aagcatcaag gaaggaacgt
    61981 aagaatgagg attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt
    62041 ttcagttggc cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc
    62101 tgatttagct gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc
    62161 atctcctcca gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg
    62221 acgaccaccg atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa
    62281 cgaataacat caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc
    62341 acaatggcaa gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac
    62401 ttcatcatca ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc
    62461 cttcacgaca acctgcccca ataataccca attctgacag ttacgttata aattgtgtga
    62521 gaaatgcgga attcccctca acgacctact ccgttggagg tagccacgga cgtcccagcg
    62581 ccgatccctg tacgccatgt tcattcagat agcgatccag ctcgactatt cccgtccatc
    62641 gattttcgca ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt
    62701 ggtagatcac ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtataatcct
    62761 ccagttcaat accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca
    62821 caatatgcag cggatgcagc tttatgccgt ccacgctggt ttcaaccact cgctccagcg
    62881 tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac aattaagtgg gaacacacct
    62941 tcagcccgcg ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc
    63001 ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt
    63061 cgtaaccctg gtccttatat tcgcaaagca gatccagcac cacatccggc acgcagtccg
    63121 ggcgggtacc aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag
    63181 aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc
    63241 gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct
    63301 gctgcgcttc atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg
    63361 taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc
    63421 cataacgacg ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct
    63481 gatagaccgc gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga
    63541 cctgggtcaa tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa
    63601 tttcatgcac aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct
    63661 tatggatttt aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa
    63721 tgctggtttt ctcgtcagtt caaggcagga taagggttaa cacgccttta tgacagtcag
    63781 gaattgactg tttctctaac gactctcctt ttagccttaa agataaaatc tattttaatt
    63841 tcagctagtt aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat
    63901 ttgacctgta tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca
    63961 gcctgctcat catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg
    64021 aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac
    64081 gctctttctg tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag
    64141 gttcacgata tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc
    64201 gcccacatag aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc
    64261 cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa ccggcgacgg ttgcggcttg
    64321 ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta
    64381 gcaaaaaact acgctgtcgg gatgctcttc ctgaataaag atcctgaact cgccgctgcc
    64441 gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct tgtcgattgt gggctggcgt
    64501 gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccacgcatt
    64561 gagcaaattt ttgtgaacgc cccggcaggc tggcgtccac gcgatatgga gcgccgtctg
    64621 tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt
    64681 agcctgtcga atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc
    64741 ttttatctgg atcttgcgga cctgcgtctg gaatcggcca tttgcctgtt ccaccagcgc
    64801 ttctccacta acaccgtacc gcgctggccg ctggcgcaac cgttccgcta tctggcgcat
    64861 aacggtgaaa tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa
    64921 ttccagacgc cgctgatccc tgacctgcac gacgccgcgc cattcgtcaa cgaaaccggt
    64981 tctgactcca gttccatgga taacatgctg gaactgctgc tggcaggcgg gatggatatc
    65041 atccgcgcca tgcgcctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg
    65101 gaactgcgtg ccttctttga ctttaactcc atgcatatgg agccgtggga tggtccggcg
    65161 ggcatcgtga tgtccgatgg tcgcttcgct gcctgtaacc tcgaccgtaa cggtctgcgt
    65221 ccggcacgct acgtcattac caaagacaag ctcatcacct gcgcctctga agtcggtatc
    65281 tgggattacc agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg
    65341 gtgatcgaca ctcgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa
    65401 agccgccacc cttataaaga gtggatggag aaaaacgtcc gccgactggt accgtttgaa
    65461 gatctgcccg atgaagaagt gggtagccgc gaactggacg acgacacgct tgccagctac
    65521 cagaaacagt ttaactacag cgcggaagag ctggactccg taattcgcgt actgggcgaa
    65581 aacggtcagg aagcggtcgg ttcgatgggc gatgataccc cattcgccgt gctctccagt
    65641 cagccgcgca ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca
    65701 atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg
    65761 aacgtctttt gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg
    65821 ctctactccg atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg
    65881 ctggatatca cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctatgc
    65941 gacaaagccg aaaaaatggt acgtagcggc accgtgttgc tggtgctctc cgaccggaat
    66001 atcgctaaag atcgcctgcc ggttccggct ccgatggcgg ttggcgcgat ccagacccgt
    66061 ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc
    66121 gatccgcacc acttcgccgt gttgctgggt ttcggcgcga cggctattta tccgtacctt
    66181 gcctatgaaa cgctgggccg cctggtagac acccacgcga ttgccaaaga ttatcgtacc
    66241 gtgatgctca actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg
    66301 ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctacac
    66361 gatgatgtag tgggcctgtg cttccagggc gcggtcagcc gcattggtgg ggcaagcttt
    66421 gaagacttcc agcaggatct gctgaatctg tcgaaacgtg cctggctggc gcgcaagccg
    66481 attagccagg gcggcctgct gaaatacgtc cacggcggcg aataccacgc ctacaacccg
    66541 gacgtggtgc gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa
    66601 tacgcgaagc tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg
    66661 ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc
    66721 tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg
    66781 gaagcgatga atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg
    66841 cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt
    66901 actccggcgt atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag
    66961 ccgggcgaag gtggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc
    67021 tattcggtgc ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc
    67081 gaagacttag cgcagcttat tttcgacctc aagcaggtta acccgaaagc gatgatctcc
    67141 gttaagctgg tgtccgaacc aggcgttggc accatcgcga ctggcgtggc gaaagcttat
    67201 gcggacttga tcaccatcgc gggctatgac ggcggcaccg gcgcaagccc gctgtcgtca
    67261 gtgaaatatg caggctgccc gtgggaactc ggtctggtgg aaacccaaca ggcgctggtt
    67321 gccaacggct tgcgtcataa aattcgtttg caggttgatg gcggcctgaa aacgggtgtc
    67381 gatatcatca aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccaatg
    67441 gtggcgctcg gctgtaaata tctgcgtatt tgccacctga acaactgcgc cacgggtgta
    67501 gcaactcagg atgacaaact gcgtaagaat cactatcacg gcctgccgtt caaggtgact
    67561 aattactttg agtttatcgc ccgtgaaacc cgcgagctga tggcacagct gggagtaacg
    67621 cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc
    67681 aaacagcaga aactggcgct gtcgaagctg ctggagactg ccgagccaca tgcaggtaag
    67741 tcactctact gcactgaaaa caacccgccg tttgataacg gcctactgaa cgcgcagttg
    67801 ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatc
    67861 cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct atatcgccca gacgcacggc
    67921 gatcaggggc tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt
    67981 ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat
    68041 gtcggtaaag gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc
    68101 cgcagccatg aagcaagcat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt
    68161 ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc
    68221 gtggtagaag gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt
    68281 ctgggtaaaa ccggcgttaa cttcggcgcg ggcatgaccg gcggcttcgc ttacgttctc
    68341 gatgaaagcg gtgatttccg caaacgcgtt aacccggaac tggtcgaagt cttaagcgtc
    68401 gacgatctgg cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat
    68461 accggttctc agcgcggtga agagattctg gcgaactggt caaccttcgc cacgaaattc
    68521 gcgctggtta aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc
    68581 gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat
    68641 caatttatcg acctgcagcg cgttgatccg ccaaagaaac cgctgaagat ccgtaaaatt
    68701 gagtttgttg aaatttacga gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc
    68761 tgtctgtcgt gcggcaaccc atactgcgag tggaaatgcc cggtacacaa ctacatccca
    68821 aactggctga agctcgccaa cgaggggcgt atttttgaag cggcagaact gtcgcatcag
    68881 accaacacac tgccggaagt atgcggacgt gtctgcccgc aagaccgtct gtgcgaaggt
    68941 tcctgcaccc tgaacgatga gttcggtgcg gtgaccatcg gcaacattga gcgttatatc
    69001 aatgataaag cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt
    69061 aaaaaagtgg cgattatcgg tgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg
    69121 cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc
    69181 ttcggtattc cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc
    69241 accggcatgg gtattgaatt caaactcaat accgaagtgg gccgcgacgt acagctggac
    69301 gatctgctga gtgattacga tgccgtgttc cttggcgttg ggacttatca gtcaatgcgc
    69361 ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc
    69421 aacaccaaac agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa
    69481 ggcaaacgcg tggtagtact tggcggtggc gacactgcga tggactgcgt gcgcacgtct
    69541 gtacgccagg gggcaaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg
    69601 ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc
    69661 cagccactgg ggattgaagt gaacggtaac ggcaaagtca gcggcgtaaa aatggtacgt
    69721 accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc
    69781 gaacatatcg ttccggcaga tgcggtgatc atggcgtttg gtttccgtcc acacaacatg
    69841 gaatggctgg caaaacacag cgtcgagctg gattcacaag gccgcatcat cgctccggaa
    69901 ggcaacgaca acgctttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc
    69961 cgtggttccg atctggtcgt taccgccatc gccgaaggtc gcaaagcggc agacggcatt
    70021 atgaactggc tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg
    70081 ctgaaattct cctttctttc tggaaatcgc ctcgcataat ctgaagaaaa ccaatgtaat
    70141 acgctcatct ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg
    70201 cagcgccata taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac
    70261 cagcaaatcc acaacggcat tattcatctg gtcgatagcg cccgaacgga aacggtacgt
    70321 agcgttaacg cgctgatgac cgcgacgtac tgggaaattg gccgacggat tgtcgaattt
    70381 gaacaaggtg gtgaggccag agcagcttat ggtgcacagt tgattgatcg gctatcgaag
    70441 gatttaagtc gacggtataa acgtggggtc tctgcaaaaa acttacgcca aatgaggctt
    70501 ttttacctct tttttcaaca tgttgaaatt cgccagacag tgtctggcga attaacacca
    70561 ttgggaattc cgcagacacc atctgcagaa ttcccatcgg caaagatttg gcagacgctg
    70621 tctgccaaat tgagaggctg tttgatattt ccacagcttt tgtattgcat ctcctatttt
    70681 cggtttgtat attcatgcaa
//
LOCUS       DAJTRB010000005        67496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTRB010000005 DAJTRB010000000
VERSION     DAJTRB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.005"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..228
                     /locus_tag="OO330_000383"
     CDS             <1..228
                     /locus_tag="OO330_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5881394.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            648..1263
                     /locus_tag="OO330_000384"
                     /pseudo
     CDS             648..1263
                     /locus_tag="OO330_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1366..1689
                     /locus_tag="OO330_000385"
     CDS             1366..1689
                     /locus_tag="OO330_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5881395.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO330_000386"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO330_000386"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5881396.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO330_000387"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO330_000387"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5881397.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO330_000388"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO330_000388"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5881398.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO330_000389"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO330_000389"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5881399.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO330_000390"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO330_000390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5881400.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO330_000391"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO330_000391"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5881401.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO330_000392"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO330_000392"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5881402.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO330_000393"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO330_000393"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5881403.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO330_000394"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO330_000394"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5881404.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO330_000395"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO330_000395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5881405.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO330_000396"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO330_000396"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5881406.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO330_000397"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO330_000397"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5881407.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO330_000398"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO330_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5881408.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO330_000399"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO330_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5881409.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19269..20486
                     /gene="alaA"
                     /locus_tag="OO330_000400"
     CDS             19269..20486
                     /gene="alaA"
                     /locus_tag="OO330_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5881410.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20570..21169
                     /gene="yfbR"
                     /locus_tag="OO330_000401"
     CDS             20570..21169
                     /gene="yfbR"
                     /locus_tag="OO330_000401"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5881411.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21228..23060)
                     /locus_tag="OO330_000402"
     CDS             complement(21228..23060)
                     /locus_tag="OO330_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5881412.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OO330_000403"
     CDS             complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OO330_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5881413.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23808..24302)
                     /locus_tag="OO330_000404"
     CDS             complement(23808..24302)
                     /locus_tag="OO330_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5881414.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24385..24840)
                     /locus_tag="OO330_000405"
     CDS             complement(24385..24840)
                     /locus_tag="OO330_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5881415.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25178..26380
                     /gene="ackA"
                     /locus_tag="OO330_000406"
     CDS             25178..26380
                     /gene="ackA"
                     /locus_tag="OO330_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5881416.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26455..28599
                     /gene="pta"
                     /locus_tag="OO330_000407"
     CDS             26455..28599
                     /gene="pta"
                     /locus_tag="OO330_000407"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5881417.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDTPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28789..30309
                     /gene="yfcC"
                     /locus_tag="OO330_000408"
     CDS             28789..30309
                     /gene="yfcC"
                     /locus_tag="OO330_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5881418.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLTPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OO330_000409"
     CDS             complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OO330_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5881419.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OO330_000410"
     CDS             complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OO330_000410"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5881420.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OO330_000411"
     CDS             complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OO330_000411"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5881421.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32329..32976
                     /gene="yfcG"
                     /locus_tag="OO330_000412"
     CDS             32329..32976
                     /gene="yfcG"
                     /locus_tag="OO330_000412"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5881422.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPPDGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33033..33395
                     /gene="folX"
                     /locus_tag="OO330_000413"
     CDS             33033..33395
                     /gene="folX"
                     /locus_tag="OO330_000413"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5881423.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33416..34309
                     /locus_tag="OO330_000414"
     CDS             33416..34309
                     /locus_tag="OO330_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5881424.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OO330_000415"
     CDS             complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OO330_000415"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5881425.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OO330_000416"
     CDS             complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OO330_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5881426.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTCLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36225..36941)
                     /locus_tag="OO330_000417"
     CDS             complement(36225..36941)
                     /locus_tag="OO330_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5881427.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTVLAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OO330_000418"
     CDS             complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OO330_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5881428.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OO330_000419"
     CDS             complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OO330_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5881429.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OO330_000420"
                     /pseudo
     CDS             complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OO330_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
     gene            complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OO330_000421"
     CDS             complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OO330_000421"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5881430.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OO330_000422"
     CDS             complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OO330_000422"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5881431.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OO330_000423"
     CDS             complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OO330_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5881432.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OO330_000424"
     CDS             complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OO330_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5881433.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OO330_000425"
     CDS             complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OO330_000425"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5881434.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OO330_000426"
     CDS             complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OO330_000426"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5881435.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45790..46449)
                     /locus_tag="OO330_000427"
     CDS             complement(45790..46449)
                     /locus_tag="OO330_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5881436.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OO330_000428"
     CDS             complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OO330_000428"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5881437.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47344..48357)
                     /locus_tag="OO330_000429"
     CDS             complement(47344..48357)
                     /locus_tag="OO330_000429"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5881438.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OO330_000430"
     CDS             complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OO330_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5881439.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49658..50653
                     /gene="flk"
                     /locus_tag="OO330_000431"
     CDS             49658..50653
                     /gene="flk"
                     /locus_tag="OO330_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5881440.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50650..51828)
                     /locus_tag="OO330_000432"
     CDS             complement(50650..51828)
                     /locus_tag="OO330_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5881441.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OO330_000433"
     CDS             complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OO330_000433"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5881442.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53483..55489
                     /gene="mnmC"
                     /locus_tag="OO330_000434"
     CDS             53483..55489
                     /gene="mnmC"
                     /locus_tag="OO330_000434"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5881443.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55610..55888)
                     /locus_tag="OO330_000435"
     CDS             complement(55610..55888)
                     /locus_tag="OO330_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5881444.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OO330_000436"
     CDS             complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OO330_000436"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5881445.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56470..57279)
                     /locus_tag="OO330_000437"
     CDS             complement(56470..57279)
                     /locus_tag="OO330_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5881446.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OO330_000438"
     CDS             complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OO330_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5881447.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OO330_000439"
     CDS             complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OO330_000439"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5881448.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OO330_000440"
     CDS             complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OO330_000440"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5881449.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60325..60876
                     /gene="smrB"
                     /locus_tag="OO330_000441"
     CDS             60325..60876
                     /gene="smrB"
                     /locus_tag="OO330_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5881450.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMYGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60980..61870)
                     /locus_tag="OO330_000442"
     CDS             complement(60980..61870)
                     /locus_tag="OO330_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5881451.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61857..62381)
                     /locus_tag="OO330_000443"
     CDS             complement(61857..62381)
                     /locus_tag="OO330_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5881452.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62378..62848)
                     /locus_tag="OO330_000444"
     CDS             complement(62378..62848)
                     /locus_tag="OO330_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5881453.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62845..63393)
                     /locus_tag="OO330_000445"
     CDS             complement(62845..63393)
                     /locus_tag="OO330_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5881454.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63398..64120)
                     /locus_tag="OO330_000446"
                     /pseudo
     CDS             complement(63398..64120)
                     /locus_tag="OO330_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64140..64862)
                     /locus_tag="OO330_000447"
     CDS             complement(64140..64862)
                     /locus_tag="OO330_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5881455.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64922..66781)
                     /locus_tag="OO330_000448"
     CDS             complement(64922..66781)
                     /locus_tag="OO330_000448"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCR5881456.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(66863..67426)
                     /locus_tag="OO330_000449"
     CDS             complement(66863..67426)
                     /locus_tag="OO330_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5881457.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16864 a        18260 c        16743 g        15629 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgagaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctatgaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcaac
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtagcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac
    19141 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    19201 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    19261 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    19321 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    19381 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19441 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19501 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19561 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19621 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19681 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19741 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa
    19801 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19861 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19921 ctgagcatca ttcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac
    19981 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    20041 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    20101 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    20161 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    20221 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    20281 tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc
    20341 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    20401 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20461 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20521 tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20581 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20641 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20701 ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20761 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20821 cttcaatgcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact
    20881 ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc
    20941 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    21001 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    21061 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    21121 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    21181 ctgcgtcgca tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    21241 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    21301 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa
    21361 gacaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa
    21421 ggcgacactc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21481 gcggtattag agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21541 atatgtggcc cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct
    21601 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21661 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21721 caggcgatga tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt
    21781 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21841 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21901 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21961 ccttccagcg ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga
    22021 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    22081 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    22141 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    22201 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    22261 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    22321 tccagccgtt gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    22381 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22441 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22501 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22561 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22621 ctaatcagcc cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22681 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac
    22741 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22801 gccactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22861 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22921 tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22981 tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc
    23041 caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc
    23101 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    23161 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    23221 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg
    23281 ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa
    23341 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
    23401 gctcagcggt tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg
    23461 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
    23521 tgagtaaggc gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt
    23581 gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
    23641 gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
    23701 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
    23761 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
    23821 atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta
    23881 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
    23941 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
    24001 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
    24061 tcctgcaaat tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata
    24121 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
    24181 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
    24241 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
    24301 attgttgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct
    24361 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
    24421 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
    24481 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
    24541 agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact
    24601 taaggcgaac agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca
    24661 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
    24721 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta
    24781 atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
    24841 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
    24901 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
    24961 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
    25021 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
    25081 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
    25141 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
    25201 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
    25261 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
    25321 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
    25381 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
    25441 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
    25501 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
    25561 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
    25621 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
    25681 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
    25741 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
    25801 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
    25861 ctctatgggt ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
    25921 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
    25981 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
    26041 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
    26101 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
    26161 tgcattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
    26221 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
    26281 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
    26341 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
    26401 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
    26461 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
    26521 gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag
    26581 ccgcgtaccg gtggcgatac gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
    26641 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
    26701 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
    26761 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
    26821 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
    26881 gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc
    26941 aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag
    27001 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
    27061 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg
    27121 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc
    27181 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
    27241 agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
    27301 cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc
    27361 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
    27421 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc
    27481 ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag
    27541 gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag
    27601 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa
    27661 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct
    27721 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
    27781 cgtgttgcag cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa
    27841 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
    27901 gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa
    27961 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
    28021 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
    28081 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
    28141 gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc
    28201 gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta
    28261 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
    28321 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac
    28381 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
    28441 acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt
    28501 atgcgcaaac cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
    28561 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt
    28621 tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt
    28681 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
    28741 cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
    28801 actgaatcca aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
    28861 gttgctattt taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag
    28921 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
    28981 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
    29041 acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    29101 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    29161 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    29221 gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta
    29281 tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg
    29341 ctgggctatg acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt
    29401 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    29461 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg
    29521 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    29581 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    29641 ggtgactggc tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg
    29701 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    29761 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    29821 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    29881 gggattttgc tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc
    29941 ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg
    30001 ttgctcttcc aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta
    30061 actatgccgt tactgacacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg
    30121 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta
    30181 atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc
    30241 ctgcttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc
    30301 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    30361 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    30421 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg
    30481 acttcatctt cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag
    30541 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    30601 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga
    30661 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    30721 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    30781 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    30841 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    30901 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    30961 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    31021 ccataacccg ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa
    31081 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    31141 tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt
    31201 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga
    31261 tccacttcgc tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc
    31321 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    31381 aacacgtcgc caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca
    31441 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    31501 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    31561 cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata
    31621 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    31681 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    31741 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc
    31801 ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc
    31861 gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    31921 aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    31981 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    32041 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    32101 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc
    32161 atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg
    32221 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg
    32281 cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc
    32341 tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt
    32401 gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg
    32461 cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctcc cgatggcggc
    32521 gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    32581 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    32641 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa
    32701 accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta
    32761 aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt
    32821 gcctgctggc cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg
    32881 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta
    32941 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    33001 ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    33061 taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    33121 aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag
    33181 atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga
    33241 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    33301 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    33361 attcggtatc catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa
    33421 catagtgatc accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga
    33481 gctgggccat caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg
    33541 ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    33601 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga
    33661 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgctag
    33721 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    33781 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    33841 ccgctgggaa gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    33901 aaccggcgtg gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg
    33961 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    34021 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    34081 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca
    34141 tcgcccggct attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc
    34201 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    34261 gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca
    34321 gcaaacatcc gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc
    34381 gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat
    34441 ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa
    34501 tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc
    34561 aataaatgca cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt
    34621 tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc
    34681 caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg
    34741 ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata
    34801 tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata
    34861 aggacttctg caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc
    34921 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
    34981 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
    35041 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
    35101 tggttctagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
    35161 attaataaaa ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
    35221 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
    35281 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
    35341 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
    35401 ccccttgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
    35461 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
    35521 cttcttctat tttcccctga tgtaggaaaa tgacgtgagt agaaacatgg cgagcaaagc
    35581 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
    35641 gtaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
    35701 cttctggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
    35761 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca
    35821 ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
    35881 cgttttccaa caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
    35941 atgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
    36001 gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga
    36061 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
    36121 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt
    36181 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
    36241 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
    36301 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
    36361 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcaca
    36421 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
    36481 gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct
    36541 tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
    36601 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
    36661 aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac
    36721 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
    36781 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
    36841 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
    36901 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
    36961 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca
    37021 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
    37081 gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
    37141 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
    37201 tgatccgccg aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc
    37261 ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa
    37321 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
    37381 cctccgtcac catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca
    37441 agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
    37501 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
    37561 tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca
    37621 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
    37681 cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa
    37741 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
    37801 cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
    37861 cagtttttca tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt
    37921 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
    37981 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
    38041 ccagtgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
    38101 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc
    38161 ggcagcgtac agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag
    38221 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
    38281 gttttcgaca aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat
    38341 atcgaaacca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
    38401 ggtaccgatg cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc
    38461 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
    38521 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
    38581 aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgcatttca
    38641 atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca
    38701 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
    38761 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
    38821 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
    38881 ctgatgagcc agcaaaagcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
    38941 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
    39001 aatcctggtt ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt
    39061 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
    39121 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
    39181 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt
    39241 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acctaggtac
    39301 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
    39361 cacctttagc atctttcgac gagaacagcg cataggtggt atcggttccg atacgtaccg
    39421 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag
    39481 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
    39541 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    39601 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    39661 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    39721 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    39781 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
    39841 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata
    39901 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
    39961 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
    40021 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    40081 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
    40141 gcatcgagcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    40201 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
    40261 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
    40321 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
    40381 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
    40441 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
    40501 taacgtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    40561 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
    40621 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
    40681 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    40741 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    40801 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
    40861 gtttttatcg cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg
    40921 cagctgctgg cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg
    40981 gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg
    41041 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    41101 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
    41161 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    41221 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    41281 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    41341 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
    41401 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    41461 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
    41521 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    41581 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    41641 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    41701 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    41761 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    41821 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    41881 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    41941 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc taggcaagaa
    42001 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat
    42061 cagctgtgat ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa
    42121 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    42181 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    42241 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    42301 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    42361 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgtgga tcaggctaac
    42421 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
    42481 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    42541 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    42601 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
    42661 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
    42721 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
    42781 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
    42841 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
    42901 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
    42961 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
    43021 cgcaggtggt gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc
    43081 gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag
    43141 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
    43201 cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcacgatcgc ccgctttcgg
    43261 caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc
    43321 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
    43381 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
    43441 aagaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg
    43501 cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
    43561 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
    43621 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
    43681 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
    43741 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
    43801 cgctggcaat cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg
    43861 ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
    43921 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
    43981 cccgacgttg taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt
    44041 ccggctcacc gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa
    44101 tactttcgcg atctggaccc agccagtcgg tatgatccag cgcaatactg gttactaccg
    44161 cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
    44221 tcaccacgtc aagttgtgcc tgcttgaaca gccacaacgc cgacagcgta ccgtactcga
    44281 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
    44341 ccgattccgg caattcctgg ccctgcacac gtacgcgctc ggtataacgc accagatgag
    44401 gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
    44461 aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagga
    44521 cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
    44581 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
    44641 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
    44701 gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta
    44761 cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc
    44821 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
    44881 tcaggaattc actgcgctgg aatctaggcg gcagtttttc gcgaacggtc tgttcgataa
    44941 cacgcggacc ggcaaagccg attaacgctt taggttcagc gatgttgaga tcgcccagca
    45001 tggcgaaact tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca
    45061 agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca
    45121 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat
    45181 cttcctgcgc ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac
    45241 cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt
    45301 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt
    45361 tatacttctt ggagtcacga aacttcagct catctttcgg ctcaagctcg ctacccagct
    45421 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    45481 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    45541 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    45601 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    45661 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    45721 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    45781 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tgacggatta tttcgatgac
    45841 gcccggcaaa atagaaacca caataatccc gacgatcagc agcttaaggt tatcctgaac
    45901 catcgggatt gtaccgaaga aatagcctgc gtaggtaaaa agcagtaccc acaacagtgc
    45961 gccgatcacg ttataagcag cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa
    46021 cggggcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg
    46081 tttctcataa aactgatggg ttttgtcgag ataactgcga cggaaaattt tcgaattggg
    46141 attactgaat aacttttcac cgaacaaccg cccgatagtg taattgaccg cgtcacccac
    46201 aatcgcggcg atcagcatca gcactaccat catatggacg ttgagatcgt tggtttccag
    46261 tgatgccaac gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcattaccac
    46321 cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga cgccgtactc
    46381 cgcgactagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    46441 caggtccata tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    46501 acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg
    46561 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    46621 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    46681 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    46741 gatgtacaaa ggcattcgct ttgatatcaa ccaccacata aggaccgtga cgcgtgacat
    46801 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    46861 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    46921 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    46981 tgatgtagcg ataacggcga gccgtggcgc taaatcgggc atgaaaatca tcaggtacag
    47041 ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc aaggtccacg
    47101 ccgcgtcttt gcgctgcgcg gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc
    47161 ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    47221 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact
    47281 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg
    47341 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    47401 gccaaagcga acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc
    47461 attacgcacg cagccaacag aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg
    47521 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    47581 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    47641 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    47701 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag
    47761 catattaaac gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag
    47821 caatttcgca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    47881 aatcaggctg gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc
    47941 cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc
    48001 ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa
    48061 cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc
    48121 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc
    48181 ggcatcctgc acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    48241 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    48301 ggcttcgccc atagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    48361 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    48421 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    48481 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    48541 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg
    48601 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc
    48661 agcgtaatgc ggccaaactc aggcgcgggc agcaacgtat ccagcgcaac gtgttgttca
    48721 tgcccaataa acttgctata ggcttcaaac acttgcgtcg tgccacgcgc tttaccctcc
    48781 agggtatagc ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta
    48841 agttccggct cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg
    48901 caggtcagca gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    48961 ccgggcttca ggctacggat cagtttttca tccgccagat gtagcgtttt gtacggacca
    49021 tctttaaaga gtggcgtatg gaaagtcaga atatccgcgc gctggactaa ctcatccagc
    49081 gagcggaaat caccctcatc cccacggtcg gcgcgaggcg gatcgcaaag taaggtttta
    49141 atccctaacg cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc
    49201 cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag cagggaggag
    49261 aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    49321 gcctgcttca accatgcttc atcgacatgg tcggtccccg cagtggcagt tccaacaaat
    49381 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    49441 gcatccgcgt ctgccagttg agcgacgggg attggacgcc cgggaaccgc gatcacctca
    49501 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg
    49561 tttgtgttac ctgtatgaga cgagagttaa ccggacaagt atgccataat ctcgcggcca
    49621 ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc
    49681 tcctcctggg cagccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc
    49741 tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac
    49801 gcaacaacaa agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga
    49861 ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct
    49921 ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg
    49981 tctggggaat catcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc
    50041 gctgaatcag ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa
    50101 gatggttatt ccgcaaccac agcagcgcga ggcgaccgac cgtcctttat taccggcgga
    50161 gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa
    50221 acagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc
    50281 gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa
    50341 tacgccgacg ctagaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact
    50401 ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc
    50461 ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga
    50521 tccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa
    50581 tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg
    50641 gctggttcgt taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga
    50701 gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag
    50761 cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc
    50821 gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga
    50881 acatccggct ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca
    50941 gagcagcaac aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc
    51001 aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc
    51061 ggtaagagta aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt
    51121 cccgataacc gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag
    51181 cccgacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt
    51241 gccgttacag gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa
    51301 accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat
    51361 cgccattccg ttccatgaca tcactttgcc agagtgtttt ggccctacga tgcctaaccc
    51421 ccaggtcaga gcgcctgtca gtaactggct ttcaccaaac ccaagaatca aacgcccgat
    51481 aaccaacagg gcaaatttga acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc
    51541 gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg cgccatattg
    51601 atcggccagt cgcccggcgt aaccacgcgt cagcaccgta gccagaaact gaatcccgac
    51661 ggcaatgccg accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac
    51721 cggcaacggc aaccctacgg tcatgtaggt gagaaaaacc gcaaaagaga tgcggaagag
    51781 cgaaaaactg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag
    51841 aatgcagcgc aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacggtgc
    51901 gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc
    51961 ctagaactgg tggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg
    52021 ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg
    52081 acctactccg gcctactgcg aattaatctt ttcagcttgc gcattaccag cgtggcgttg
    52141 gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg
    52201 gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg
    52261 ataaagccgt gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc
    52321 agtgagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    52381 tcacggatag ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc
    52441 aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct
    52501 tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca
    52561 gcatagatgt aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg
    52621 ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc
    52681 ggggtgtcgt tgtatttagt agatagcgca cccatcgcgt cgaattcgca ggccatttcc
    52741 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    52801 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    52861 acgccatgaa ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta
    52921 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    52981 aacacctgga aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta
    53041 ttctggtaag cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat
    53101 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    53161 tccagtttta tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag
    53221 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    53281 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    53341 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    53401 atcagccatt taatagagat atttgcgcag ccttacacac atcgctaaga tcgagccacc
    53461 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    53521 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    53581 cgggctggaa gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc
    53641 tgagcatcca catcctctgt ttgtgatagc agagagcggc ttcggcaccg gattaaactt
    53701 cctgacgcta tggaaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    53761 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    53821 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    53881 gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg
    53941 gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga
    54001 tgcctggttt ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct
    54061 gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc
    54121 cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt
    54181 tgggcgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc
    54241 cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg
    54301 tattgccagc gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta
    54361 ttgcgcggat gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc
    54421 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc
    54481 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    54541 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc
    54601 cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    54661 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    54721 taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    54781 gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    54841 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac
    54901 tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc
    54961 agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    55021 acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    55081 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa
    55141 agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    55201 tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt
    55261 ggcggagcaa aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc
    55321 ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca
    55381 gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg
    55441 gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc
    55501 agtgtttgcc ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga
    55561 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg
    55621 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    55681 attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    55741 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    55801 acccactggc aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca
    55861 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctgac
    55921 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    55981 aattttgtag tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat
    56041 ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt
    56101 ccagattgtc gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga
    56161 acgcctgcgg cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt
    56221 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    56281 agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat
    56341 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    56401 tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg
    56461 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    56521 taatttggca ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc
    56581 tttgctcaac accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac
    56641 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt
    56701 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    56761 cataacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa
    56821 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    56881 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg
    56941 caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga
    57001 gccgacaaag gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    57061 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    57121 cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc
    57181 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    57241 cagcggagaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    57301 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc
    57361 ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt
    57421 aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc
    57481 gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    57541 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    57601 gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta
    57661 ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    57721 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    57781 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    57841 agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa
    57901 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca
    57961 gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    58021 tgggttattt tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc
    58081 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    58141 ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg
    58201 atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact
    58261 tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    58321 gccatatgag caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg
    58381 ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact
    58441 acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    58501 gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg
    58561 gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt
    58621 tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc
    58681 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca
    58741 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    58801 gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg
    58861 taggtgtaat cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa
    58921 cgctggtcgg tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa
    58981 acaccggaga gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc
    59041 ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc
    59101 ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg
    59161 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    59221 gtttacttaa tctttataaa tcgcgaaatg ttctcgtgcg gcaataagct gctctttagt
    59281 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg
    59341 atattgttcc ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc
    59401 agcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc
    59461 agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    59521 cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg
    59581 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    59641 ttcggcaacc gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa
    59701 ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg
    59761 cggttgcttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg
    59821 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt
    59881 caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg
    59941 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta
    60001 gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta
    60061 ccagatattt gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg
    60121 cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    60181 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    60241 tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg
    60301 aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    60361 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac
    60421 cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    60481 gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg
    60541 aaatatgttc gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat
    60601 tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg
    60661 ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgtatggg
    60721 cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg
    60781 atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt
    60841 gaagtggaag agtggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca
    60901 gcgtaaacgc cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc
    60961 gtaggcctga taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact
    61021 gaaaacgaca cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat
    61081 cgtgtagtac tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat
    61141 cacaaccgta agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc
    61201 catcattatc agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg
    61261 aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga
    61321 cataattttc actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt
    61381 tatgcactgt aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg
    61441 atgcgatcca agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg
    61501 cacaacgaaa aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt
    61561 cagtcagtac caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat
    61621 cgcctttata cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg
    61681 taacattgaa aacactgctc gctacagcac cggttacctt gtccatggca taataaactc
    61741 tcacactgtc aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga
    61801 caacactcag aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag
    61861 aaatactcaa aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg
    61921 ctctttgcct gtaaccaggt attaaaattt aggctgttat ttccctgatt cagcgggagc
    61981 gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc
    62041 ccagcgccgg atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct
    62101 tctgttcctg tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat
    62161 tgaaacacca atggcactcg ctctgactgc ccaaggttca ttaaatcact ggcggcaatg
    62221 gcaggaaaat gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg
    62281 attaaattgc cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac
    62341 atcaggctac taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg
    62401 tggcccaggc ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag
    62461 ccgttaacac cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct
    62521 gccctgcctg tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat
    62581 caaaatcgct tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg
    62641 ccgtatcgca ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat
    62701 caataagcac atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag
    62761 gcggctgcac taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc
    62821 acagcaaaat aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt
    62881 aaattcagct gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg
    62941 aactcccgag gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg
    63001 atcactactt ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc
    63061 accgttaatc acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt
    63121 gttacaaaca agcgagtagt caatgggacg tttataatca accctattga tcttattaat
    63181 gaatacatta ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac
    63241 aagcacccgt aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac
    63301 acaacatatc agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct
    63361 cccggcctca gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag
    63421 tgaaaaagag cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac
    63481 ccatcttcac attcagcggt gcagaggttt tcggcgacat cactattggc gagaaaccgg
    63541 ctgccggatt gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg
    63601 ggttattgac ggtaaattcc tgaccgttgc gggttaaagt aatcttatat tgctaggggt
    63661 ttttcatgct gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta
    63721 gtgctatttg cagcgtgttg gctttgtttg attttggcgg gatctcacgc acgttgaagt
    63781 agaacaggct ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta
    63841 cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac
    63901 tgatcttgtt acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat
    63961 cgttattgcg caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac
    64021 gatctggcgt tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg
    64081 tcgtagcacc caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt
    64141 tattggttag ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc
    64201 tgaatgacag gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat
    64261 ggcagtaaca agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa
    64321 tgcgcaacac cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa
    64381 acattgccgt catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg
    64441 ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg
    64501 atgacggcaa atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac
    64561 tgggttgctt cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa
    64621 tagttattga cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg
    64681 ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc
    64741 atattctggg tacggtgaag tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac
    64801 gacaagcccg cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc
    64861 agcgaaccat catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg
    64921 ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc
    64981 gtcccactgc catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga
    65041 gcgaaatgta catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg
    65101 agatgctgac attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt
    65161 ttgtctgctc ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac
    65221 ccgcatcgcg gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc
    65281 gtaccattcc gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg
    65341 agaagcgata tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac
    65401 tcacacggaa ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt
    65461 tggtgtgcga gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga
    65521 caccaagcgc cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc
    65581 cgttagcgat cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc
    65641 cccactcttg cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt
    65701 atggcatcga ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc
    65761 gaatatgcaa cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg
    65821 gaacctgcgt ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt
    65881 gtgccacgcc ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt
    65941 cagtactgac actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat
    66001 cctcgcccag cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac
    66061 tccactccca ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag
    66121 tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa
    66181 ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt
    66241 gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg
    66301 gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg
    66361 actggcttaa atcagcctta atttccatgc cttccagttg accggatttc aggcatttag
    66421 catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca
    66481 gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa
    66541 tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg
    66601 gctcaacata tccctggctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt
    66661 ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg
    66721 acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca
    66781 tacacgtttc tatccgtttt tattcttgtt cggtcaaaat acatttaaat cccccatagc
    66841 agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa
    66901 aattacccag atctggcgca tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct
    66961 gcttcgcttt gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc
    67021 cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg
    67081 caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac
    67141 cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct
    67201 ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca
    67261 tatcaacttc cagtttgtga tcgtctggaa cgatagaaca cactgagtct tcaatggtgc
    67321 cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc
    67381 ccatcaccat tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa
    67441 ctataaataa ttaaaaatat aaacattgca atacattgat tcagtcaata gccaat
//
LOCUS       DAJTRB010000006        66827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTRB010000006 DAJTRB010000000
VERSION     DAJTRB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.006"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            52..156
                     /locus_tag="OO330_000450"
     CDS             52..156
                     /locus_tag="OO330_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5881458.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            467..2664
                     /gene="speF"
                     /locus_tag="OO330_000451"
                     /pseudo
     CDS             467..2664
                     /gene="speF"
                     /locus_tag="OO330_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2661..3980
                     /gene="potE"
                     /locus_tag="OO330_000452"
     CDS             2661..3980
                     /gene="potE"
                     /locus_tag="OO330_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5881459.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4036..5676)
                     /gene="pgm"
                     /locus_tag="OO330_000453"
     CDS             complement(4036..5676)
                     /gene="pgm"
                     /locus_tag="OO330_000453"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5881460.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5702..6247)
                     /gene="seqA"
                     /locus_tag="OO330_000454"
     CDS             complement(5702..6247)
                     /gene="seqA"
                     /locus_tag="OO330_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5881461.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6432..7196
                     /gene="ybfF"
                     /locus_tag="OO330_000455"
     CDS             6432..7196
                     /gene="ybfF"
                     /locus_tag="OO330_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5881462.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7336..7629
                     /gene="ybfE"
                     /locus_tag="OO330_000456"
     CDS             7336..7629
                     /gene="ybfE"
                     /locus_tag="OO330_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5881463.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7769..8299
                     /gene="fldA"
                     /locus_tag="OO330_000457"
     CDS             7769..8299
                     /gene="fldA"
                     /locus_tag="OO330_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5881464.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8509..8595
                     /gene="uof"
                     /locus_tag="OO330_000458"
     CDS             8509..8595
                     /gene="uof"
                     /locus_tag="OO330_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5881465.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8588..9034
                     /gene="fur"
                     /locus_tag="OO330_000459"
     CDS             8588..9034
                     /gene="fur"
                     /locus_tag="OO330_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5881466.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9118..9444)
                     /locus_tag="OO330_000460"
     CDS             complement(9118..9444)
                     /locus_tag="OO330_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881467.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9481..10887)
                     /gene="chiP"
                     /locus_tag="OO330_000461"
     CDS             complement(9481..10887)
                     /gene="chiP"
                     /locus_tag="OO330_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5881468.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11466..13130)
                     /gene="glnS"
                     /locus_tag="OO330_000462"
     CDS             complement(11466..13130)
                     /gene="glnS"
                     /locus_tag="OO330_000462"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5881469.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWLDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13333..15279)
                     /gene="nagE"
                     /locus_tag="OO330_000463"
     CDS             complement(13333..15279)
                     /gene="nagE"
                     /locus_tag="OO330_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5881470.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            15612..16412
                     /gene="nagB"
                     /locus_tag="OO330_000464"
     CDS             15612..16412
                     /gene="nagB"
                     /locus_tag="OO330_000464"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5881471.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16472..17620
                     /gene="nagA"
                     /locus_tag="OO330_000465"
     CDS             16472..17620
                     /gene="nagA"
                     /locus_tag="OO330_000465"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5881472.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17629..18849
                     /gene="nagC"
                     /locus_tag="OO330_000466"
                     /gene_synonym="nagR"
     CDS             17629..18849
                     /gene="nagC"
                     /locus_tag="OO330_000466"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5881473.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18897..19649
                     /gene="nagD"
                     /locus_tag="OO330_000467"
     CDS             18897..19649
                     /gene="nagD"
                     /locus_tag="OO330_000467"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR5881474.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            20046..21710
                     /gene="asnB"
                     /locus_tag="OO330_000468"
     CDS             20046..21710
                     /gene="asnB"
                     /locus_tag="OO330_000468"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5881475.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            22089..22165
                     /locus_tag="OO330_000469"
     tRNA            22089..22165
                     /locus_tag="OO330_000469"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22123..22125,aa:Met,seq:cat)
     gene            22174..22258
                     /locus_tag="OO330_000470"
     tRNA            22174..22258
                     /locus_tag="OO330_000470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22208..22210,aa:Leu,seq:tag)
     gene            22282..22356
                     /locus_tag="OO330_000471"
     tRNA            22282..22356
                     /locus_tag="OO330_000471"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22314..22316,aa:Gln,seq:ttg)
     gene            22391..22465
                     /locus_tag="OO330_000472"
     tRNA            22391..22465
                     /locus_tag="OO330_000472"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22423..22425,aa:Gln,seq:ttg)
     gene            22481..22557
                     /locus_tag="OO330_000473"
     tRNA            22481..22557
                     /locus_tag="OO330_000473"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22515..22517,aa:Met,seq:cat)
     gene            22606..22680
                     /locus_tag="OO330_000474"
     tRNA            22606..22680
                     /locus_tag="OO330_000474"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22638..22640,aa:Gln,seq:ctg)
     gene            22718..22792
                     /locus_tag="OO330_000475"
     tRNA            22718..22792
                     /locus_tag="OO330_000475"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22750..22752,aa:Gln,seq:ctg)
     gene            complement(22946..24121)
                     /gene="ubiF"
                     /locus_tag="OO330_000476"
     CDS             complement(22946..24121)
                     /gene="ubiF"
                     /locus_tag="OO330_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5881476.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            24267..25691
                     /gene="miaB"
                     /locus_tag="OO330_000477"
     CDS             24267..25691
                     /gene="miaB"
                     /locus_tag="OO330_000477"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5881477.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25844..26884
                     /locus_tag="OO330_000478"
     CDS             25844..26884
                     /locus_tag="OO330_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5881478.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26881..27348
                     /gene="ybeY"
                     /locus_tag="OO330_000479"
     CDS             26881..27348
                     /gene="ybeY"
                     /locus_tag="OO330_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5881479.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27438..28316
                     /gene="corC"
                     /locus_tag="OO330_000480"
     CDS             27438..28316
                     /gene="corC"
                     /locus_tag="OO330_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5881480.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            28341..29879
                     /gene="lnt"
                     /locus_tag="OO330_000481"
     CDS             28341..29879
                     /gene="lnt"
                     /locus_tag="OO330_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5881481.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTTLFGFAAVLMSLR
                     QRRK"
     gene            30276..31184
                     /gene="gltI"
                     /locus_tag="OO330_000482"
     CDS             30276..31184
                     /gene="gltI"
                     /locus_tag="OO330_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5881482.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            31354..32094
                     /gene="gltJ"
                     /locus_tag="OO330_000483"
     CDS             31354..32094
                     /gene="gltJ"
                     /locus_tag="OO330_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5881483.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            32094..32768
                     /gene="gltK"
                     /locus_tag="OO330_000484"
     CDS             32094..32768
                     /gene="gltK"
                     /locus_tag="OO330_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5881484.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32768..33493
                     /gene="gltL"
                     /locus_tag="OO330_000485"
     CDS             32768..33493
                     /gene="gltL"
                     /locus_tag="OO330_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5881485.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            33611..34546
                     /gene="rihA"
                     /locus_tag="OO330_000486"
     CDS             33611..34546
                     /gene="rihA"
                     /locus_tag="OO330_000486"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5881486.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            34630..36300
                     /gene="hscC"
                     /locus_tag="OO330_000487"
                     /pseudo
     CDS             34630..36300
                     /gene="hscC"
                     /locus_tag="OO330_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(36360..37811)
                     /gene="djlC"
                     /locus_tag="OO330_000488"
     CDS             complement(36360..37811)
                     /gene="djlC"
                     /locus_tag="OO330_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5881487.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QVGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37808..38515)
                     /locus_tag="OO330_000489"
     CDS             complement(37808..38515)
                     /locus_tag="OO330_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5881488.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            38617..39171
                     /locus_tag="OO330_000490"
     CDS             38617..39171
                     /locus_tag="OO330_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5881489.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(39181..40608)
                     /locus_tag="OO330_000491"
     CDS             complement(39181..40608)
                     /locus_tag="OO330_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5881490.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(40605..41312)
                     /locus_tag="OO330_000492"
     CDS             complement(40605..41312)
                     /locus_tag="OO330_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5881491.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            41476..42453
                     /locus_tag="OO330_000493"
     CDS             41476..42453
                     /locus_tag="OO330_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5881492.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(42523..43005)
                     /locus_tag="OO330_000494"
     CDS             complement(42523..43005)
                     /locus_tag="OO330_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5881493.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            43240..45822
                     /gene="leuS"
                     /locus_tag="OO330_000495"
     CDS             43240..45822
                     /gene="leuS"
                     /locus_tag="OO330_000495"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5881494.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            45837..46418
                     /gene="lptE"
                     /locus_tag="OO330_000496"
     CDS             45837..46418
                     /gene="lptE"
                     /locus_tag="OO330_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5881495.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKDMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            46418..47449
                     /gene="holA"
                     /locus_tag="OO330_000497"
     CDS             46418..47449
                     /gene="holA"
                     /locus_tag="OO330_000497"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5881496.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            47451..48092
                     /gene="nadD"
                     /locus_tag="OO330_000498"
     CDS             47451..48092
                     /gene="nadD"
                     /locus_tag="OO330_000498"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5881497.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            48116..48727
                     /locus_tag="OO330_000499"
     CDS             48116..48727
                     /locus_tag="OO330_000499"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5881498.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            48987..49304
                     /gene="rsfS"
                     /locus_tag="OO330_000500"
                     /gene_synonym="rsfA"
     CDS             48987..49304
                     /gene="rsfS"
                     /locus_tag="OO330_000500"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5881499.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            49308..49775
                     /gene="rlmH"
                     /locus_tag="OO330_000501"
     CDS             49308..49775
                     /gene="rlmH"
                     /locus_tag="OO330_000501"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5881500.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            49806..51707
                     /gene="mrdA"
                     /locus_tag="OO330_000502"
     CDS             49806..51707
                     /gene="mrdA"
                     /locus_tag="OO330_000502"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5881501.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPYEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            51710..52822
                     /gene="mrdB"
                     /locus_tag="OO330_000503"
                     /gene_synonym="rodA"
     CDS             51710..52822
                     /gene="mrdB"
                     /locus_tag="OO330_000503"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5881502.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            52833..53921
                     /gene="rlpA"
                     /locus_tag="OO330_000504"
     CDS             52833..53921
                     /gene="rlpA"
                     /locus_tag="OO330_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5881503.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            54060..55271
                     /gene="dacA"
                     /locus_tag="OO330_000505"
     CDS             54060..55271
                     /gene="dacA"
                     /locus_tag="OO330_000505"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5881504.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            55382..55645
                     /locus_tag="OO330_000506"
     CDS             55382..55645
                     /locus_tag="OO330_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5881505.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            55746..56387
                     /gene="lipB"
                     /locus_tag="OO330_000507"
     CDS             55746..56387
                     /gene="lipB"
                     /locus_tag="OO330_000507"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5881506.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            56646..57599
                     /locus_tag="OO330_000508"
     CDS             56646..57599
                     /locus_tag="OO330_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5881507.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            57808..58773
                     /gene="lipA"
                     /locus_tag="OO330_000509"
     CDS             57808..58773
                     /gene="lipA"
                     /locus_tag="OO330_000509"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5881508.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(58874..59077)
                     /gene="tatE"
                     /locus_tag="OO330_000510"
     CDS             complement(58874..59077)
                     /gene="tatE"
                     /locus_tag="OO330_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5881509.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(59206..59994)
                     /locus_tag="OO330_000511"
     CDS             complement(59206..59994)
                     /locus_tag="OO330_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5881510.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            60087..60470
                     /gene="crcB"
                     /locus_tag="OO330_000512"
     CDS             60087..60470
                     /gene="crcB"
                     /locus_tag="OO330_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5881511.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(60524..60733)
                     /gene="cspE"
                     /locus_tag="OO330_000513"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(60524..60733)
                     /gene="cspE"
                     /locus_tag="OO330_000513"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5881512.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(60908..61468)
                     /gene="pagP"
                     /locus_tag="OO330_000514"
     CDS             complement(60908..61468)
                     /gene="pagP"
                     /locus_tag="OO330_000514"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5881513.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            62057..63442
                     /gene="dcuC"
                     /locus_tag="OO330_000515"
     CDS             62057..63442
                     /gene="dcuC"
                     /locus_tag="OO330_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5881514.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(63483..63824)
                     /locus_tag="OO330_000516"
                     /pseudo
     CDS             complement(63483..63824)
                     /locus_tag="OO330_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(64162..64671)
                     /locus_tag="OO330_000517"
     CDS             complement(64162..64671)
                     /locus_tag="OO330_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5881515.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            64834..65079
                     /locus_tag="OO330_000518"
     CDS             64834..65079
                     /locus_tag="OO330_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5881516.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            65054..66676
                     /locus_tag="OO330_000519"
                     /pseudo
     CDS             65054..66676
                     /locus_tag="OO330_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
     gene            66687..66827
                     /locus_tag="OO330_000520"
                     /pseudo
     CDS             66687..66827
                     /locus_tag="OO330_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
BASE COUNT        16712 a        16760 c        16704 g        16651 t
ORIGIN      
        1 catggataga ataactctac cttcaactga cacagcaagc ggtaaaggta aatggaaaat
       61 aataaccgct taatgcctca tataaggcgg acaacccata tcatgaagtt tgcccatcgt
      121 aatagttttg actttcattt ctttaatgcc cgctagtctt ctgactaaag ggcaccccaa
      181 cgtacaggtc tccctgactt taagcattac aggttaatac ctgtattcct cggtgctcat
      241 atactgctaa cccttttaaa ctctaaataa ttcgagtcgc agcacttgca acttgaggta
      301 tgacgagtat agccagttac cgggctggtc tgggttattg catctgcaaa aagcaaacta
      361 ctgatttatt tatcagcggt ggagctttgc tttttttccg acgtgatcga tttctccttt
      421 gagaaattga ggacctgcta ttacctgaaa taaagagatg aataaaatgt cagaattaaa
      481 aattgccgtt agccgttctt gcccggattg tttttccact catcgtgcat gcgtgaatat
      541 tgacgaaagt aattatattg acgttgccgc cattatttta tcagtcaatg atgttgaacg
      601 tggaaaactc gataaaatag acgctactgg ctatggcatt cctgttttta ttgcaacgga
      661 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
      721 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      781 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      841 ttttgactgc ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca
      901 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      961 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
     1021 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
     1081 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
     1141 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
     1201 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
     1261 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
     1321 cgctgtgatt cagttaggca cctacgacga tacgatttat aacgcccgcc aggtggtgga
     1381 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
     1441 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
     1501 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
     1561 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
     1621 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1681 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1741 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1801 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1861 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1921 agagaatcaa tactttgtcg atccttgcaa actgttgctg acaaccccag gtattgatgc
     1981 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     2041 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     2101 ggcagaagat atggccaaac ttcagcaact tgtgccctgc tggtacgctt cgaaaaactg
     2161 cttgagtccg acgcgccatt agcagaagtg ctgccttcca tctacaaaca gcatgaagag
     2221 cgctacgccg gttataccct gcgtcagttg tgccaggaaa tgcatgattt gtatgcccgc
     2281 cacaacgtga aacaactgca aaaagagatg ttccgcaaag cgcacttccc acacgtcagc
     2341 atgaatccgc aagaagccaa ctacgcctat ttacgcggtg aagtggagct ggttcgtctg
     2401 ccggatgccg aaggtcgtat cgctgccgaa ggggcgcttc cttatcctcc gggtgtgctg
     2461 tgtgttgttc cgggtgaaat ctggggtggt gctgttttgc gttacttcag cgctctggaa
     2521 gaagggatca acctgctgcc aggttttgca ccggagctgc agggtgtcta tatcgaagaa
     2581 catgatggtc gtaagcaagt ttggtgctat gtcatcaagc ctcgtgatgc gcaaagcacc
     2641 ctgttgaaag gggaaaaatt atgagtcagg ctaaatcgaa caaaatgggc gtcgttcagt
     2701 taaccatact gacgatggtc aacatgatgg gctccggtat catcatgctg ccgacaaagc
     2761 ttgccgaagt tgggacaatt tcaattatct cctggctggt gacagccgta ggctcaatgg
     2821 cactggcatg ggcattcgca aaatgcggta tgttcagccg taaatcaggt ggtatgggcg
     2881 gttatgccga gtatgctttc ggtaaatccg gtaactttat tgcgaactat acctacggcg
     2941 tctcactgct gattgctaac gtcgcgattg ctatttcggc ggttggttac ggcaccgaac
     3001 tgctcggcgc gagtttgtcg ccagtgcaga ttggtcttgc gaccatcggg gtgctgtgga
     3061 tttgtaccgt ggctaacttt ggtggtgcgc gcattaccgg gcaaatcagt agcattaccg
     3121 tgtggggggt cattattccg gtggtaggcc tgtgcattat cggctggttc tggtttagcc
     3181 cgacgcttta cgttgattcc tggaatccgc atcatgcacc gttcttcagt gcagtaggtt
     3241 cttccatcgc tatgacgctg tgggcttttc ttggtctgga gtctgcgtgt gcgaatactg
     3301 atgtagtgga aaacccggaa cgtaatgtgc caatcgcggt actcggcggt acgttaggtg
     3361 cggcggtgat ttatatcgtc tccaccaacg tgattgcagg gattgtgcct aatatggagc
     3421 tggcaaattc aacggcacca tttggcctgg ccttcgcaca gatgtttaca ccagaagtgg
     3481 gtaaagtcat tatggcgctg atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt
     3541 tcaccattgc ccaggtgttt aaatcttcat ctgatgaagg ctacttccct aaaattttct
     3601 cccgtgtaac caaagtggat gcaccggtgc agggaatgtt gaccattgtg attatccaga
     3661 gcgggttggc gctgatgacg attagcccgt cgctaaacag tcagttcaac gtgctggtta
     3721 acctggccgt ggtgaccaat atcattccgt atattctgtc aatggcggca ttagtcatta
     3781 ttcagaaggt cgctaatgtg cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg
     3841 ttggcgcgat gtatagcttt tatgcgctgt actcatccgg ggaagaagcc atgctgtacg
     3901 gttccatcgt gaccttcctt ggttggacac tgtatggtct ggtatcacca cgctttgaac
     3961 tgaaaaataa acacggttaa aaaagcaaag taaaaaaggg cgatcttgcg accgctcttt
     4021 ttttattaaa tgtgtttacg cgtttttcag aacttcgcta acaatctcaa ccgcttcttt
     4081 ctcaatctgc ttgcgatgtt cttcaccgag gaagctttcg cagtagatct tatatgcgtc
     4141 ttccgtgcct gacggacgcg cggcgaacca gccgttgtca gtcatcactt tcagaccgcc
     4201 aatagaagca ccgttgcccg gagcagcagt caggcgcgcg gtgatcgggt cacctgccag
     4261 ggtgctggcg ctcaccattt ccggagacag cttagacagc gccgcttttt gtgcggaagt
     4321 cgcagctgcc tgcaaacggt tgtagctcgg cgcaccaaag cgttttgcca gttcgttgta
     4381 gtgttcctgc gggttcttac cggtgacagc ggtgatttcc gccgccagca gacacataat
     4441 gatgccgtct ttgtcggtgg accacggcgt gccgtcgaaa cgcaggaagg aagcccctgc
     4501 gctctcttcg ccgccaaagc cgaagctgcc gtcgaacaga ccatcaacaa accatttgaa
     4561 acctaccggt acttctacca gcttacggcc caagtcattg accacacggt cgatcatcgc
     4621 agaagaaacc agcgttttac cgacagcaac atctttgccc cactgcggac gatgctggaa
     4681 caggtagttg atcgccaccg ccaggtagtg gttcggattc atcaaacctg ccggagtgac
     4741 gataccgtga cggtcataat ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag
     4801 tgccagcaga cccgccatcg cacactcgga ggagcagtcc atacggatcg caccgtcttt
     4861 atcgaggtgc ataaagcgga aggtttgatc gacctgatcg ttaacgatgg tcaggttgag
     4921 gttgtaatac tcgccaatgc gcttccagta ttcgataccg gaaccgccca acggatcaac
     4981 gcccagcgtc aggcccgctt tctgaatcgc cgccatatca acgatatcgg ccagcccttc
     5041 cacgaacggc tgcaccagat cctgctcttt cacatagccg gatgccatcg cttcgtcgag
     5101 ggagatacgc ttcacgcctt tcaggccatc ggccatcagt gcgttggccc tgtcttccac
     5161 cactttggtg acgttagtat cagccgggcc accatttggc ggattgtact tgataccacc
     5221 atcttccggc gggttatggg acggtgtaat cacgataccg tctgccagcg ggccaccttt
     5281 tttattgtga accaggatgg cattggaaac ggcaggcgtc ggagtgaagc cattgttttc
     5341 ctgcacaatg acatcaacgc cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg
     5401 ctcggacagg gcgtgagtat ctttacccac atagcaaggg ccagtgatgc cgtttttcgc
     5461 acgttcttca gcaattgcct gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg
     5521 cgctgcactg ccacggtgac cggaagtacc gaatttcacc gcgtgctccg cattccctgc
     5581 ttctggtttc agtacataat attgcgccgt cagttgggcg acgttaatca aatcactctg
     5641 ttgtgcaggt tggcctgcac gattgtggat tgccattgct ttgtcctttg tctgcaacgt
     5701 tttagatagt tccgcaaacc ttctcaatca attccgccgg gaattgcatc gactgcatga
     5761 tgtgttcgat catgctgcat ttacggccgg tgttggtgtt ggtgatcacc caatacggcg
     5821 tgcctggcac atgtttcggc ttggtctgat taccattttt cagcagcgtt tgttcatctg
     5881 ccgcaaagta cacgcgtgta cgaccgtgca acgattccgt tgcttcggca aacgcctggg
     5941 cgtcaagaga atatagtgta gacaacagca gcataaagcg attgaccgct cgcttttgct
     6001 ctgcgtattc gtccgaaagc agaagttcac gcattgcgcg aaccttgtct ttaatcgttt
     6061 tgaccggctt cgcttcgacg atagcaggtg acgcaacgcg aacctctttc gtcaccggag
     6121 cagcaggctg tgatgcggcg gaaaatttca acatacgccg taaaatgtcg gatgcgctct
     6181 cgccgatatg cttagtgtgg ctggcaatat agctgtagag ttcatcatca acttcaatcg
     6241 ttttcatctt aatccagtgc agtgtcttag ctgaatacaa gtcgccaaga gtatatgaat
     6301 aaatcccggc agcggatagc gtcaagcctg gcttgcagaa aaactcggaa taaaccttat
     6361 ttgttgcagc cctgtggcac aatggtcaac ataataccct aacccgacaa cgcgaaaaaa
     6421 agaactttgc catgaaatta aatatccgcg cgcaaactgc acaaaaccag cacaataatt
     6481 ctcccatcgt tcttgtccat ggtctgtttg gtagcctcga caaccttggc gtactggctc
     6541 gcgatctggt aaacgatcac aatatcatcc aggttgatat gcgtaaccac ggtctttcac
     6601 cgagagagcc ggtaatgaat tacccggcga tggcgcagga tcttgttgat accctggatg
     6661 cactgcagat cgacaaagca acatttatcg gtcactccat gggcggtaaa gcggtaatgg
     6721 cacttactgc actggccccc gatcgcatcg ataaactggt ggcgatcgat atcgcaccgg
     6781 tcgactatca cgtacgccgt catgatgaga ttttcgcagc tatcaacgcg gtcagtgaat
     6841 cggacgcaca aactcgccag caagcagcag caataatgcg ccagcatctt aatgaagaag
     6901 gggtgattca gtttctgttg aaatcttttg ttgacgggga gtggcgcttt aacgtgccgg
     6961 tattgtggga tcagtatccg catattgtgg gttgggagaa aatcccggca tgggatcacc
     7021 ctgccctgtt tatccctggc ggcaattctc cgtatgttag cgagcagtac cgtgatgatt
     7081 tactggcaca atttccacag gcacgggcgc atgtgattgc aggcgcgggt cactgggtcc
     7141 atgctgaaaa accggatgcg gtattacgcg ctatccgtcg ctatctcaat gattaacgca
     7201 actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt gggcgttggc gtgactcatc
     7261 gcgctgatgt attatggcgc gctatccatc cgggcagagg cctggctgat tgtttccccc
     7321 gaagtcacca agatcatggc caaagaacaa acggaccgta cgacattaga tctgttcgcg
     7381 cacgagcgtc gaccgggacg accgaaaact aatccgcttt cgcgcgatga acagctgcgt
     7441 attaataaac gcaaccagct aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg
     7501 aagctgaacg cggaagctgt cgaggcgctg aacgagctgg cggaatcgcg taatatgagc
     7561 cgtagcgaac tgatcgaaga gatgttaatg cagcaactgg cggcgctgcg tagtcagggc
     7621 atcgtttaaa tttccacttt catgtagcac agtgtgcagt cctgctcgtt tgctgattcc
     7681 gcaagactgc ctgttctgct atgattgcct ttatccgtgg gcaattttcc acccccattt
     7741 caataagttt caagaggtta tttcactcat ggctatcact ggcatctttt tcggcagcga
     7801 caccggtaat accgaaaata tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc
     7861 cgatgtccat gacattgcaa aaagcagcaa agaagatctg gaagcttacg acattctgct
     7921 gctgggcatc ccaacctggt attacggcga agcgcagtgt gactgggatg acttcttccc
     7981 gactctcgaa gagattgatt tcaatggtaa actggttgcg ctgtttggtt gtggtgacca
     8041 ggaagattac gccgaatatt tctgcgacgc attgggcacc atccgcgaca tcattgaacc
     8101 gcgcggtgca accatcgttg gtcactggcc aactgcgggc tatcatttcg aagcatcaaa
     8161 aggtctggca gatgacgacc actttgtcgg tctggctatc gacgaagacc gtcagccgga
     8221 actgaccgct gaacgtgtag aaaaatgggt taaacagatt tctgaagagt tgcatctcga
     8281 cgaaattctt aatgcctgat gtgatgcggc gtagactcat gtctacgccg tattaataga
     8341 taatgccaat caaaataatt gctacaaatt tgtaactttt gctgttgtac ctgtacaatg
     8401 tcccagtgtt caagtggcct tgccgttgta aatgtaagct gtgccacgtt tttattaaca
     8461 atatttgcca gggacttgtg gttttcattt aggcgtggca attctataat gatacgcatt
     8521 atctcaagag caaattctgt cacttcttct aatgaagtga accgcttagt aacaggacag
     8581 attccgcatg actgataaca ataccgccct aaagaaagct ggcctgaaag taacgcttcc
     8641 tcgtttaaaa atcctggaag ttcttcagga gccggacaac catcacgtca gtgcggaaga
     8701 tttatacaaa cgtctgatcg atatgggtga agaaattggt ctggctacgg tatatcgcgt
     8761 actgaaccag tttgacgacg ctggtatcgt cacccgccac aattttgaag gcggtaaatc
     8821 cgtatttgaa ctgacacagc aacatcacca cgatcacctg atttgcctcg actgcggcaa
     8881 ggttatcgaa tttagtgatg attccatcga agcgcgtcag cgtgaaattg ccgcaaaaca
     8941 tggcattcgc ctgactaacc acagtctcta tctttacggt cactgtgccg aaggcgattg
     9001 ccgcgaagat gagcacgcgc acgaaggcaa ataagccagc ctgaacgaga aaagccaacc
     9061 tgcgggttgg cttttttata caagggaaat aagaaaagcg agagttacag ctctcactta
     9121 tttgttatta ctgcgtattt cctgccagac cttatcacaa tcggctttca ccgcctgatc
     9181 atttccggtt tgcgttgcac gcagacactg ctgataatcc agtacgcgta cactttcctg
     9241 gtcggcaaac tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc
     9301 ttcaattttt tctggcgaac cttgcgcggt gttgatacag gcgctgtatg cctctttcag
     9361 acggctgtct tcctgtggtg cggttgattg cgcacaagct accagccccg atgccatcat
     9421 ggcgatgaga atcagttttt tcattgaatg cggcaggcac aaaacctgcc gcgtcgggca
     9481 tcagaagatg gtgaatggtg cgattaccat aaatttcacg tcacgctcat cctggaagat
     9541 gttaccgtaa ccgccgcccc agcttgggat gtcggagtgg ttgtcgtatt cggtgaagtg
     9601 cagtttgaac atcgtgcctt tggcgcgacc gtcctgaatg gtgtagaccg catccaggct
     9661 gtaggcagac tcttcaatag tccggttttt gtcgtagtac gcatccggat tgctctgcca
     9721 ggtcgcaggt ttagcatccc atgcgtaaac gtaggaagcg ccgatggcga agcctggaag
     9781 attccagttt ttcaggtcat acatcgcact gaagaagacc gctttttcgc cgttagcgtt
     9841 gaagtcggaa cggttatccc accagatatc caggcggccg ttggaggaag cgtaggttgg
     9901 ggtcatacgt tgcaggaagt atccctgctg actgtcagcc ttaatccagg tgccttcgag
     9961 gcgcaaatcc actacgtcag ccgcccggta accaaaggtc aacgcttgca gccaggcggt
    10021 gccgtcataa agatcgttga cgctgcgatc gtcaacttta tcgcgggtac catagaactg
    10081 gtagctggtg gttaacgggc taccggcgat atcaaatttg tagctggctt tggcaaaata
    10141 ttgatcgata tacccttccg cctgaccaaa tgccgcttcc agtacgaagt tatttttgaa
    10201 gtcgtatttc gcaccgatgg agtgcagata atcaactttg gtggttttat cgttctgata
    10261 aaactcatcc atttccagat gccacggcgc tttgtattcg ttggtccaca tgtaggagaa
    10321 actcaaggca ccagcatcgc cgtaatcaaa attcgccccg gcttccgccc cctgataagt
    10381 acccggcata aagctccagt gcggcgctaa cagcgtttga ccggttggct gaatgtaacc
    10441 tgccctcgcc caaaccggac cgtatttaaa tttggccgca gctttataca ggcttatacc
    10501 gcttttgtcg ccggaccagt cttcgtcata ggctttatta ctttttgaaa acgcgatttc
    10561 gttcgggtgg gagctgtcgc cgttttccgc catttcaatc gccgtaaacg cagcaatatc
    10621 aaggccgaac atatcagcag cataaccgga ctgaaaatcg aggttggcat tccaggtgga
    10681 gtgagaaagg ttggttttgt atttgtcgcc gtcggtaaca tctttacggt cgcgttcacg
    10741 ctgccagtaa tagataccgc cggttaaggt tgaatcgtcg atgaatcctt cggcgcgagc
    10801 ctccggcatt gccataaagc ccgacattgc tgtaacaccg gcaatagcca gcgccagcgt
    10861 actacgtttg ccactaaacg tacgcatggg ttaatcctct ttgacgtata aattgctgca
    10921 ccaaaggtga acagcgaaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    10981 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    11041 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttatgtttg
    11101 cggttgaaag gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac
    11161 attttattta attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga
    11221 attaattcgc gaagaaaagc agaaaaaacg ccgcagagcg cggcgtttgg gattgtttgc
    11281 tatttcttgc cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaattcaa
    11341 tatattgcag agatcatgta ggcctgataa gcgaagcgca tcaggcaatt tagcgtttga
    11401 aatttattgt ctatttattg ccggatgcga cgtaaatgcc tcacccggct tggtagaact
    11461 taaaattact cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc
    11521 ggtttttcgg ccgtggaatg gcggctgtcg aggcagaagt aaccttcacg ctcaaactgg
    11581 aatgctttac ccgcaaccgc atctttcagc gacggttcag caaagccctg tttgatcacc
    11641 agcgattccg ggttaatcac cgacaggaaa tcatccgcag cacctgggtt aggcacgctg
    11701 aacagacgat catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga
    11761 ataacacctt tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa
    11821 gtacagaaga tggtggtgat attaccttcg gcatctttct ccacgcgttc agccttaatg
    11881 acgtaagcat tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta
    11941 gcttcttcgc ggaaatcggc gcgatcgagc caaatttcac cgctaaacgg cacctgacgg
    12001 ctccccattt ccggtttgtt cggatggttc ggcatggtga ccatttcgcc ttcgccctga
    12061 tagttttcga taaccagttt caccggatcg ataaccgcca ttgcgcgcgg cgcattttcg
    12121 ttgagatctt cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg
    12181 gtcacgccga tgcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc
    12241 agaccggaaa tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc
    12301 agcaggttca gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg
    12361 tactggcgcg gatgaacagg aatggtgatg ttatccagta cccagtcgta cagacgacgg
    12421 ttgtcctgga actcaagcgt acacagagag tgcgtaatac cttccagcgc atcgctgatg
    12481 caatgggtga agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca
    12541 gcaaatttaa tgcggtacag caccggatcg cgcattacga taaatggcga cgccatgtcg
    12601 attttcgcac gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac
    12661 agcgccaagt tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggctgcgtc
    12721 agggtaccgc ggtattcgcg gatctgttcc ggtgtcagtt catcaacgta cgccaggcct
    12781 ttattgatca gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg
    12841 acgttaccag accagtgaaa acctaaccac tctacgtcgt ttttgatcga atcgacatac
    12901 tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag
    12961 tcctgggcga tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca
    13021 ttcggctccg gcgggaaacg ggtgtgtact gtgatgtgct taccactggc cagatcttca
    13081 tcgatgatct gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc
    13141 tcaaagcgta aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg
    13201 ttagtttttt aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag
    13261 cggcagagaa gatctccacc gcttgtcatt gttggatgcg acgctgccgt gtcgcatcag
    13321 gcataaagca gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc
    13381 ctgagcttta atgagcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat
    13441 catcgagcgg gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc
    13501 gcttacctgc gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt
    13561 gtcgataccc atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg
    13621 gttggtgttg aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt
    13681 cggtttcacc gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg
    13741 atccagtgcc acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt
    13801 tggtacagcc tgcggttttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc
    13861 agcggctacc ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt
    13921 cgcgccaaca atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag
    13981 acgtttacac atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt
    14041 aatacaggca tcaatcgctt tcaggttgtc agtaccgcca accgcagcaa tatagttggt
    14101 tgccagttga gtcagacctt cttcagtatt gctgttggct tcttcagtaa cgatctcgtc
    14161 ttctttatct tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac
    14221 cacgaagtag atagcgaaga agataacgcc catcaccagc agcatccaga cgttctggct
    14281 ggcggcaggc aggttataca tcaacgcgta gtcgatagca cccgcagaga aagagaagcc
    14341 ggcatggata cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag
    14401 gaggtacagc agcggcgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt
    14461 caggaacgca gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt
    14521 cggtgctgcg aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa
    14581 gaagccggac atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc
    14641 gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt
    14701 cagcacctga tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga
    14761 acccagcgcg cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg
    14821 cggccagacg taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg
    14881 cacaaagcgt ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg
    14941 gttataggct gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat
    15001 ttctgggttg atgatcacca tcgctttggt taacacaaag taacctaccg cacccgccag
    15061 cgccgccgca cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa
    15121 tgcgaggtta tcaaaaattg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc
    15181 tggctgaccg aatcgcagca acagcgccgc caccggcagc accgcgatag ggagctgtaa
    15241 cgccctaccg agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc
    15301 ctatttggct cgtttaaagc cgtattttta ttttgctgca aattttactg ccgatgttct
    15361 gtaatcagat tgttagatca tctgctacag agtgtgtgaa aatttaattc gcatcgcaaa
    15421 ttaaacgcgt gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac
    15481 tggctaaacc agaaaactta ttttatcatt caaaaaatca agtcggattg acgcctgtct
    15541 gcgcaaatcc aggttacgct taaagatgtc taatccgcca acggcttaca ttttacttat
    15601 tgaggtgaat aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg
    15661 ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccaac tgccgatcgt ccgtttgtac
    15721 tgggcctgcc gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata
    15781 aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc
    15841 tgccgaaaga gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg
    15901 ttgatattcc agcagaaaac atcaaccttc tcaacggcaa tgccccggat atcgatgccg
    15961 agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg
    16021 gtgtaggtaa cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta
    16081 ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg
    16141 atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt tggtacactg ctggatgccg
    16201 aagaagtgat gattatggtg ctgggtagcc agaaagcact ggcgctgcag gccgccgttg
    16261 aaggttgcgt gaaccatatg tggaccatca gctgtctgca actgcatccg aaagcgatca
    16321 tggtgtgcga tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg
    16381 aattagaagc agaaaatatc aaaggtctgt aattgttatc cctgccctgg ttccttgctc
    16441 agggcaatat tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt
    16501 taccggccac gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag
    16561 cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat
    16621 tctctccccc ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga
    16681 caccgctgaa gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc
    16741 aggctgtact aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg
    16801 cgtgcgcgtt atgcgtgagt acctggcaaa acatccgaat caggcgttag gtctgcatct
    16861 ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa
    16921 gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac
    16981 cctggcaccg gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt
    17041 ggtttctgcc ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg
    17101 gattaccttt gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg
    17161 cctggcgggc gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct
    17221 gcatgttgat tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct
    17281 ggttaccgac gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa
    17341 aacaatatac taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc
    17401 cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga
    17461 agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt gacgttgaaa aacgtctcgg
    17521 cacgctcgcc gcaggtaaag tagccaacct gaccgcattc acacctgatt ttaaaatcac
    17581 caagaccatc gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc
    17641 ggacaagctc agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat
    17701 cgcctgattg accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt
    17761 gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa
    17821 gttgatcagc aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc
    17881 aatttccacg caatcggcgt acggcttggt cgtcatgacg ccaccatcac tctgtttgat
    17941 ctcagcagca aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg
    18001 ctggaacatg ccctgctgaa tgccattgct cagtttattg atagctacca gcgtaagctg
    18061 cgcgagctaa tcgccatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa
    18121 attcattaca tgccgcatat tcaggtagaa aactgggggc tggtagaagc actggaagaa
    18181 cgttttaaag tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac
    18241 tacttcggtg caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc
    18301 ggggccggga ttatctctaa cgggcgcatt tttattggcc gcaacggtaa cgtcggtgaa
    18361 attggccata ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc
    18421 ctggaaacta tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag
    18481 ggctaccaga gccgtgtgcc actggacgac tgcaccatca aaactatctg caaagccgcg
    18541 aacaaaggcg atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggtaaa
    18601 accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc tggtgaaatc
    18661 accgaagccg ataaagtgct gctccctgct attgaaagct gcattaatac ccaagcgctg
    18721 aaggcgtttc gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc
    18781 ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg
    18841 gaaaattaat gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga
    18901 ccattaaaaa tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac
    18961 cgggtgcagc ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga
    19021 ccaactatcc ttcgcagact gggcaagatc tggcgaaccg ctttgccact gccggtgtcg
    19081 atgtgccgga tagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc
    19141 aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag
    19201 ccggtttcac tattaccgat gtgaaccctg atttcgtgat tgttggcgaa acacgttctt
    19261 acaactggga catgatgcat aaagcagcct atttcgtcgc taacggtgca cgctttatcg
    19321 ccaccaaccc ggacacccac gggcgcggtt tttatcccgc ttgtggcgcg ttgtgtgcag
    19381 ggattgagaa aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca
    19441 tccgcgcggc attaaacaaa atgcaggcgc attcggaaga aacagtgatt gtcggcgata
    19501 acctgcgtac cgatattctg gcgggcttcc aggccggtct ggagacgatt ctggtgcttt
    19561 ctggtgtttc atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc
    19621 cgtcagtcgc tgaaatcgac gttatctgaa taccacccac gtctgcggac gtggttggct
    19681 ctgatgacaa acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc
    19741 aatatattga attttcatgc ttttgtaggc cggataaggc gttcacgccg catccggcat
    19801 gaacaaatcg cactttatca gcaatttggc ccccgtaaaa tataaccatt caataaaacc
    19861 cgctaaaaat tatcgattca tcaataaata catcctcaca ccagaccctt tttcaccaat
    19921 cgacaatcac cattacgttt tttatttttg cactgccaaa tagcttgcgc acagtgctgc
    19981 tttgcggcat ttttttaaac aagcaaacac aacaagcaac aaataccagg ttaacggaga
    20041 aggttatgtg ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta
    20101 agaaagcact cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt
    20161 atgccagcga taacgccatt ctcgctcacg aacgtctgtc aattgttgac gttaacgcgg
    20221 gggcgcaacc tctctacaac caacaaaaaa accacgtact ggcggtaaac ggtgaaatct
    20281 acaaccacca ggcattgcgc gctgaatatg gcgatcgtta tcagttccag acagggtctg
    20341 actgtgaagt gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc
    20401 agggcatgtt tgcctttgcc ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg
    20461 accatctggg gatcatccca ctgtatatgg ggtatgacga acacggtcag ctgtatgtgg
    20521 cctcagaaat gaaatcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga
    20581 gctatttgtg gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact
    20641 ttgatgcagt gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt
    20701 ccgttaaaag ccatctgatg tctgatgtgc cttacggtgt gctactttct ggtggtctgg
    20761 attcctcaat tatttccgct atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg
    20821 aacgctctga agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac
    20881 cggatcttaa agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc
    20941 acttcactgt acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt
    21001 atgatgtgac cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg
    21061 cgatgggcat taaaatggtg ctgtccggtg aaggttctga tgaagtgttt ggcggttatc
    21121 tttacttcca taaagcgccc aacgctaaag aactgcatga agagacggtg cgtaaactgc
    21181 tggccttgca tatgtatgac tgcgcgcgcg ccaacaaagc gatgtcagcc tggggcgtgg
    21241 aagcacgcgt tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccgc
    21301 aggataaaat gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgagt
    21361 catacctgcc cgcaagcgtg gcctggcggc agaaagagca gttctccgat ggcgtcggtt
    21421 acagttggat cgacaccctg aaagaagtgg cggcgcagca ggtttctgat cagcaactgg
    21481 aaactgcccg cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc
    21541 gcgagatctt tgaagaactg ttcccacttc cgagtgccgc tgagtgcgtg ccgggcggtc
    21601 cttccgtcgc gtgttcttca gctaaagcga tcgaatggga tgaagcgttc aaaaaaatgg
    21661 acgatccgtc tggtcgtgcg gttggcgttc accagtcggc gtataagtaa gaaatgcggt
    21721 acagccccgg agaaatccgg ggctgtttta gttattgcgc tgcttgttga ttctgatatg
    21781 cagtaaggaa atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca
    21841 gtatttcata gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtacgac
    21901 aaaagaatgg ctgtagggtg gggaaaaaca caaagtaaac aataattgac gaatatagcg
    21961 ccacgctgtt cgcaacctaa ccaaacagtc actttcgagc aattttcctt gaaaaagagg
    22021 ttgacgctgc aaggctctat acgcataatg cgccccgcaa cgccgataag gtatcgcgaa
    22081 aaaaagatgg ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca
    22141 ggttcgaatc ccgtcgtagc caccatcttt tttgcgggag tggcgaaatt ggtagacgca
    22201 ccagatttag gttctggcgc cgcaaggtgt gcgagttcaa gtctcgcctc ccgcaccatt
    22261 caccaggaag cgttgtacgg atggggtatc gccaagcggt aaggcaccgg tttttgatac
    22321 cggcattccc tggttcgaat ccaggtaccc cagccatctt cttcgagtaa gcggttcacc
    22381 gcccggttat tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct
    22441 ggttcgaatc caggtacccc agccatcgaa gaaataatct ggctacgtag ctcagttggt
    22501 tagagcacat cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccaaat
    22561 tctgaatgtg tcgaatacgt tcggcaaatt caaaaaccaa tttgttgggg tatcgccaag
    22621 cggtaaggca ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca
    22681 atttattcaa gacgcttaca tcgtaagtgc acccagttgg ggtatcgcca agcggtaagg
    22741 caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc cacattaaaa
    22801 aagctcgctt cggcgagctt tttgcttttc tgcgttcatt caatgtcgaa tgcgatgttg
    22861 acacgtctta tccttcaatg tcggatgcga cgctgccgcg tcttatccga cctacggctg
    22921 gcacgcatcc ggcaatgttg taaggctaca accctaacgc atatttcagc gcctgacgtt
    22981 tcaacacgcc agcacgctct gccgccatta acccgagatt acgcacaaaa cgcagcggcg
    23041 gcagattatt actgaatccg gcataaaaca gatccatacc actttgcata atgaagttat
    23101 ccgccatgcg ccgcatctgg taacgcttga ggacaggata actggcccac gcttcaccgt
    23161 agctgcgggc attcaccaga acatcaatca gggcatcgac atcacgataa ccaagattca
    23221 ccccctgccc cgccagcgga tggatggtat gcgcggcatc gcccaccagc gcaagccccg
    23281 gctgcacata ctgcaacgca tgtcggcgcg tcagcggaaa cgcaccagcg gcaagcggtg
    23341 taacgtaacc cagacgcgac gggaaatgct tcgcgatttc cgcttggagt tgcgccatat
    23401 tcatattctg caactggcga atacgcgccg gagagtcata ccacaccagc gatgcccagt
    23461 tatcaaacaa cggcagaaac gcacgcggtc cgtccggggt aaattgctgc caggtgctgt
    23521 cgccgggatc gttctcacac tgtacgctaa tcaacatgca cgactgcgca tactgccagg
    23581 catgaacgcc aattcccgcc atctgccgca cctgagaatt tgcgccgtcg gcaccaatca
    23641 ccagtttcgc gcggatcgtt tcaccgccct tcagttccag ctcctgaaga tcattatctc
    23701 gatgtaatgc aatcagcgag gttggcacac gtaacgttac tttcggatgc gcttccagcg
    23761 cctgccacaa cgcctgttgc aggactgtgt tttccaccat gtagccaagc aacggcaact
    23821 ttaattcggc tgcgtcaaat accacatgtg ccgtttccca ctcccaggtt tccagtctgc
    23881 ggtaaggatg gcaacgcata gcctgtactg catcccagac ccctaaccct ttaagcaatg
    23941 ataccgaagc cgcgctgatc gccgagatcc gcacgtccgg ttggctgtca gcgacaaacg
    24001 gtgcaggttc tgcgtgctcg atcaccgtta ccgaaaatcc gtgctgtgcc agccccagcg
    24061 ccagtgcgcc gccgaccatt cctccgccga caatggcaat ttccgttggt tgatttgtca
    24121 tggtcaatca tcctgttagc aatatgcttt aagtttacag gatttttacc cccaagggct
    24181 gataacgctc atactggtca caacggcagc aaagcattac actatgcgcc ctgcattcct
    24241 ggctactatt tcgcaagagc aagtcgatga ccaaaaaact ccatattaaa acctggggct
    24301 gtcagatgaa cgagtacgat tcatcgaaga tggccgatct gctggatgcc acccacggct
    24361 atcaactgac cgacgtggcg gaagaagcgg atgtgctact gctgaacacc tgctcaatcc
    24421 gcgagaaggc tcaggaaaaa gtcttccatc agttgggtcg ctggaaactg ttaaaagaga
    24481 agaatccaga cctgattatc ggcgtcggtg gctgcgtggc atcgcaagaa ggcgagcaca
    24541 ttcgccagcg cgcccactat gtcgatatta tttttgggcc gcaaacgctg caccgtctgc
    24601 cggagatgat caactccgtg cgcggcgacc gcagcccggt tgtagatatc agcttcccgg
    24661 aaatcgagaa gtttgaccgt ctgccggaac cgcgcgccga agggccgacc gcgtttgtct
    24721 ccatcatgga aggctgcaat aaatattgca cctactgcgt ggtgccttac acccgtggtg
    24781 aagaggtaag ccgtccgtct gacgatattc tgtttgagat tgcccagctt gcggctcagg
    24841 gcgtgcgtga agtcaacctg ctcggtcaga atgtgaatgc ctggcgtggt gagaactacg
    24901 acggcaccac cggatcgttt gccgatctgt tgcgtctggt tgctgcgatc gacgggatcg
    24961 atcgtattcg ctttaccacc agccatccga tcgaattcac cgacgatatc atcgaagtgt
    25021 accgcgacac gcctgagctg gtgagcttcc tgcatctgcc ggtacagagc ggttccgatc
    25081 gcattctgaa cctgatggga cgtacccata cggcgctgga gtacaaagcg atcatccgta
    25141 aactgcgtgc ggcgcgtccg gatattcaga tcagttctga cttcattgtt ggcttccctg
    25201 gcgaaaccac cgaagacttc gagaaaacga tgaagctgat tgccgacgtc aatttcgaca
    25261 tgagctacag ctttatcttc tctgcacgtc caggaacgcc tgccgccgat atggttgatg
    25321 atgttccgga agaagagaag aagcagcgtc tgtatattct gcaagaacgt atcaatcagc
    25381 aagcgatggc atggagccgt cggatgctcg gcaccaccca gcgtattctg gtagaaggga
    25441 cttcgcgtaa gagcatcatg gagctttccg ggcgtaccga aaataaccgc gtggtcaact
    25501 tcgagggcac accggatatg atcggtaaat tcgtcgatgt agaaattacc gacgtctacc
    25561 cgaactctct gcgcggtaaa gtggtgcgaa ctgaagatga aatgggtctg cgcatggcag
    25621 aaacaccgga gtcggtgatt gcccgcaccc gcaaagaaaa cgaccttggc gtgggttatt
    25681 atcagccgta attctcaggc ctgccgttcc ggtgggcctt gttttttctc tctttatccc
    25741 catctttatc gcaatgcttg cgcctttttc ctgtggcgtg aataatttcc ttataggccg
    25801 gtgaatgttc agcttccggc ggcatacaga taagaggaac ggtttgaaca tagacactcg
    25861 cgaaatcacc ctggagccag cagacaatgc gcgtctgttg agcctgtgcg gcccgtttga
    25921 tgacaacatc aagcagctcg aacgccgtct cggcatcgag atcaatcgcc gcgataacca
    25981 ctttaaactg accggccgtc cgatttgcgt caccgctgcg gcagacattc tgcgtagcct
    26041 gtatgtcgat actgccccga tgcgcggtca gattcaggat atcgaaccgg aacagatcca
    26101 ccttgcgatt aaagaagcgc gggtactgga gcaaagcgcg gagagcgtgc cggagtacgg
    26161 caaagcggtc aatatcaaaa ctaaacgcgg cgtaattaag ccgcgcacgc caaaccaggc
    26221 gcagtacatc gccaatattc tcgaccatga cattaccttc ggcgttggcc cggcgggtac
    26281 gggtaaaacc tacctggcag tggctgcggc agttgatgcc ctggagcgtc aggaaattcg
    26341 ccgtattctg ctgactcgtc cggcggtcga agccggtgag aaactgggct tcctgcctgg
    26401 cgatttaagc cagaaagtag acccgtatct gcgcccactg tacgacgcgc tgtttgaaat
    26461 gctgggcttt gagaaagtcg agaaactgat tgagcgcaac gttattgaag tcgcgccgct
    26521 ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc attctcgatg agagccagaa
    26581 cactaccatc gaacagatga agatgttcct gacccgtatc ggttttaact caaaagcggt
    26641 tatcaccggc gacgtcacac agatcgacct gccgcgtaat actaaatcag gcttacgtca
    26701 tgccattgaa gtgctggccg atgtcgaaga gatcagcttt aacttcttcc acagcgaaga
    26761 cgtggttcgt cacccggtgg tggcgcgtat cgttaacgct tatgaagcct gggaagaagc
    26821 agaacaaaaa cgtaaagcgg cgctggcagc agaacgcaag cgcgaagaac aggaacaaaa
    26881 atgagtcagg tgatcctcga tttacaactg gcatgtgaag ataattccgg gttaccggaa
    26941 gagagccagt ttcaaacatg gctgaatgcg gtgatcccgc agtttcagga agagtcggaa
    27001 gtgactattc gcgtggtcga taccgccgaa agccacagtc tgaatctgac ctatcgcggt
    27061 aaggataagc cgaccaacgt gctctccttc ccgtttgaag tgccgcctgg catggagatg
    27121 tcgctactgg gcgatctggt tatctgccgt caggtggttg agaaggaagc tcaggagcaa
    27181 ggcaaaccac tggaggcgca ctgggcgcat atggtggtgc acggcagtct gcatttgtta
    27241 ggttacgatc acattgaaga tgacgaagca gaagaaatgg aagccctcga aacagagatt
    27301 atgcttgctc tgggctatga ggatccgtac attgccgaga aagaataatt tgccaggcat
    27361 ttgactggca gacgtccccg ccgttggtga ttaacgacgg cgttttgatt aactaacatg
    27421 agaacccata cgacgccatg agcgacgaca attcacacag tagtgacacg ataagcaaca
    27481 agaagggatt tttctccctg ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg
    27541 acgaactgct ggcgctgatc cgtgattccg ggcagaacga ccttatcgac gaagatacgc
    27601 gcgatatgct cgaaggggtg atggacatcg cagaccaacg cgttcgcgac atcatgatcc
    27661 cccgctccca gatgattacc ctgaaacgca accagacgct ggacgaatgc cttgatgtca
    27721 tcatcgagtc cgcccactca cgtttcccgg tgattagcga agacaaagat cacattgaag
    27781 ggattctgat ggcgaaagac ttgctgccgt ttatgcgcag cgatgctgaa gccttcagca
    27841 tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag taagcgcgta gaccggatgc
    27901 tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt tattgacgaa ttcggtgggg
    27961 tttccggtct ggtgaccatt gaagacatcc tggaactgat tgttggtgag attgaagacg
    28021 agtatgacga agaagatgat atcgacttcc gtcagctgag tcgtcatacc tggaccgtgc
    28081 gcgcactggc ttccattgaa gacttcaacg aagcgttcgg cacccacttt agcgatgaag
    28141 aagtcgacac tatcggtggt ctggtgatgc aggcgtttgg gcatcttccg gcgcgtggcg
    28201 aaactatcga catcgacggt taccagttca aagtggcgat ggccgatagt cggcgtatta
    28261 ttcaggttca tgtcaaaatc ccggatgact caccccagcc aaagctggat gaataaaacc
    28321 gaaactggat agataactac atggcttttg cctcattaat tgaacgccag cgcattcgcc
    28381 tgctgctggc gttattattc ggtgcctgcg gaacgctggc cttctctcct tacgacgtct
    28441 ggcctgcggc gattatttcg ctgatggggc ttcaggcgtt gacctttaac cgtcgcccac
    28501 tccagtctgc cgctattggc ttttgctggg gatttggcct ctttggcagc ggtattaact
    28561 gggtctatgt cagcatcgcg acctttggcg ggatgcctgg cccggttaac atcttcctgg
    28621 tggtgctgct ggcggcgtat ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc
    28681 gtctgtggcc gaaaaccacc tggctgcgcg tggcgattgc cacccctgct ctctggcaag
    28741 tgaccgagtt tctgcgcggt tgggtgctga ctggcttccc gtggttacag ttcggctata
    28801 gccagattga tggcccgtta aaagggctgg caccgctaat gggcgtggaa gccattaact
    28861 tcctgctgat gatggttagt ggcctgctgg cactggcgtt ggtcaaacgc aactggcgtc
    28921 cgctggtggt ggccgtcgtg ctgtttgccc tacccttccc gctgcgttac atccaatggt
    28981 ttaccccgca accggagaaa accattcagg tttcgatggt tcagggcgat attccgcaat
    29041 cgctgaaatg ggacgaaggc cagcttctta atacgctaaa gatttactac aacgcaacgg
    29101 caccgctgat gggcaaatca tcgttgatta tctggccgga gtcggcgata accgatctgg
    29161 aaattaatca gcaaccgttc ctcaaagcac tggacggtga gttgcgtgat aaaggtagct
    29221 cgctggtaac cgggattgtc gacgcgcgtc tcaataagca gaaccgctac gatacctaca
    29281 acaccatcat cacgctgggt aaaggtgcac cgtacagcta cgaatcagcc gatcgctata
    29341 acaaaaacca tctggtgccg tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt
    29401 tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg tgggccatat atccagccgc
    29461 cgctgtcggc aaatggtatt gagcttactg cggctatttg ctacgagatc attctcgccg
    29521 agcaagtgcg cgataacttc cgcccggata ccgactacct gctgactatc tccaacgatg
    29581 cgtggtttgg taaatctatt ggtccatggc aacacttcca gatggcgcga atgcgtgcgc
    29641 tggagctggc gcgcccactg ttgcgcagca ccaacaacgg cattacggcg gtgattggcc
    29701 cgcagggtga gattcaggcg atgatcccgc agttcgcccg cgaggtgtta accactaacg
    29761 tgacgccgac caccggactc acaccatacg cacgtaccgg caactggccg ctgtgggtgc
    29821 tgacgacact gtttggtttt gctgctgtgt tgatgagtct gcgtcagcga cgtaaataat
    29881 cccttcttat cgtgccggaa tacatcccat atttcggcac gctcaccgga tgcggcatca
    29941 gtgcattatc cggataacaa tattctccta tccaactttg gcacatctat tgctttgtta
    30001 tacaaggcaa agcttgaacc agcatcagta caacccggtt gcaccacagc aatccaccgg
    30061 tagcaccaaa agagtgcatt ttaagaattt tgcttcagaa tggtgcggtg cacctcgcaa
    30121 aaacaaacaa taaaacatca atatctttac atttagccaa actaaatgtt acctaacagg
    30181 cacaacactg cacaataaag ttgcagacga taacaacaca aacactcaca acgggtatcc
    30241 atgcgttctt aacgcagaag ataaaggtgt tggatatgca attacgtaaa cctgccacag
    30301 caatcctcgc cctggcgctt tccgcaggac tggcacaggc agatgacgcc gccccggcag
    30361 caggcagtac gctggacaaa atcgccaaaa acggtgtgat tgtcgtcggt caccgtgaat
    30421 cttcagtacc tttctcttat tacgacaatc agcaaaaagt ggtgggttac tcgcaggatt
    30481 actccaacgc cattgttgaa gcagtgaaaa agaaactcaa caaaccggac ttgcaggtaa
    30541 aactgattcc gattacctca caaaaccgta ttccactgct gcaaaacggc actttcgatt
    30601 ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa acaggcggct ttctctgaca
    30661 ctattttcgt ggtcggtacg cgcctgttga ccaaaaaggg cggcgatatc aaagattttg
    30721 ccgacctgaa aggcaaagcc gtagtcgtca cttccggcac tacctctgaa gttttgctca
    30781 acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg
    30841 actctttccg caccctggaa agcggtcgtg ccgttgcctt tatgatggat gacgctctgc
    30901 tggccggtga acgtgcgaaa gcgaagaaac cagacaactg ggatatcgtc ggcaagccgc
    30961 agtctcagga agcctacggt tgcatgttgc gtaaagacga tccgcagttc aaaaagctga
    31021 tggatgacac tatcgctcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataaat
    31081 ggttcaaaaa tccaattccg ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa
    31141 tgaaagcact gttcaaagaa ccgaatgaca aggcactgaa ctaattacaa gaaccagggg
    31201 cggaaattcc agccctctcg attgttacgt agcacggaca gactatacgc ctgatggtcg
    31261 ttccccatcg ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta
    31321 acgctgctac cctctttttt ctggagtaga tttatgtcta tagactggaa ctggggtatt
    31381 tttttacaac aagccccgtt cggcaatacc acctatcttg gttgggtctg gagtggtttt
    31441 caggtgacga tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt cggttcattt
    31501 ttcggcattt tacgtaccgt tccaaaccgt tttctctccg gtctgggtac gttgtatgtc
    31561 gaactgttcc gcaacgtgcc gttgattgtg caattcttta cctggtatct ggtgatcccg
    31621 gagctgctgc cggagaaaat cggcatgtgg tttaaggccg agctggatcc caatattcag
    31681 tttttccttt catccatgct ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag
    31741 gtacgcgccg ctattcagtc gctaccgcgt gggcagaaga atgccgcgct ggcgatgggc
    31801 ctgacgctac cgcagactta tcgatacgta ctgctgccta atgcttatcg cgttatcgtc
    31861 ccgccgatga cttccgagat gatgaacctg gtgaaaaact ccgccatcgc ctcgaccatc
    31921 ggtctggtgg atatggcggc gcaggcgggt aaattgctcg actactcggc tcacgcctgg
    31981 gagtcgttta ccgccatcac gcttgcgtat gtgttgatta acgcttttat catgctggtg
    32041 atgacgctgg ttgaacgtaa agttcgcctg cccggcaaca tggggggcaa ataatgtacg
    32101 agtttgactg gagttccatt gttccttccc tgccatatct gctcgacggg ctggtgatca
    32161 ccctgaaaat caccgtcacg gcagtcgtga ttggtatttt gtggggtacc atgctggcgg
    32221 tgatgcgctt gtccagcttc gcgcctgttt cgtggtttgc caaagcctac gttaacgtat
    32281 tccgctcgat tcctttagtc atggttttgc tgtggtttta cttgattgtg ccgggatttc
    32341 tgcaaaatgt gctgggatta tcgccaaaaa atgatattcg tttaatttcg gcgatggtgg
    32401 cgttttcgat gtttgaagcg gcctactatt cagagattat ccgtgccggt attcaaagta
    32461 tttcccgtgg tcagtcgagc gccgcgctgg cgttggggat gactcactgg cagtcgatga
    32521 aactgattat tctgccgcag gctttccgcg cgatggtgcc gctgctgctc actcagggca
    32581 tcgtactgtt ccaggatacc tcgctggtgt atgtgttatc tctggccgat ttcttccgta
    32641 ccgcctcaac cattggtgag cgtgatggta cgcaggtcga gatgatcctg tttgccggat
    32701 ttgtttattt cgttattagc cttagcgcgt cgttgttggt cagctacttg aaaagaagga
    32761 cagcataatg attaccctga aaaatgtttc aaaatggtat ggtcactttc aggtgctgac
    32821 cgactgctca accgaagtga aaaaaggcga agtggtggtg gtttgcggcc cgtctggttc
    32881 cggcaaatca acgctgatta aaaccgtcaa cggcctcgaa ccagtgcagc aaggtgaaat
    32941 caccgtcgat ggtatcgtgg ttaacgacaa gaaaaccgat ctggcaaagc tgcgctcccg
    33001 cgtcgggatg gtattccagc atttcgagct gttccctcat ctgtcgatta tcgaaaacct
    33061 gaccctggcg caggtgaaag tgcttaaacg cgataaagcg cccgctcgtg aaaaagcatt
    33121 gaaattgctg gagcgcgtgg gactttccgc acatgccaat aaatttccgg cgcagctttc
    33181 cggcggtcag cagcagcgcg tggcaatcgc tcgcgcattg tgtatggctc ctattgcgat
    33241 gctgttcgac gaaccgacat cggcgctgga tccggagatg atcaacgaag tgctggacgt
    33301 gatggtggaa ctggcgaacg aaggaatgac catgatggtg gtgacccacg aaatgggctt
    33361 tgcccgcaaa gtggcgaatc gggtgatctt tatggacgag ggtaaaattg tcgaagactc
    33421 gccgaaagac gctttcttcg atgatccgaa atcggaccgc gcaaaagact tcctcgcgaa
    33481 aatcctgcat taatcactct ggcgcacata cccaattgcg cgccatctca cgctttatcg
    33541 tttcgcccct cgtatctgtt acatcctagc gttacccttg tcgcaaagaa gcacacaaca
    33601 aggagcaaca atggcactgc caattctgtt agattgcgac ccaggtcatg atgacgctat
    33661 cgcaatagtt ctcgcactcg cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc
    33721 cggaaaccag acaccagaaa aaaccttacg caatgttctg cgtatgctga ccttgcttaa
    33781 tcgcaccgat attccggtgg caggcggcgc ggtaaaaccg ttaatgcgtg agttgattat
    33841 cgcggacaat gtgcacggcg aaagcggtct cgacggcccg gcattaccgg aaccgacgtt
    33901 cgcaccgcaa aactgtacgg cggtggagct gatggcgaaa acgctgcgcg aaagtgcgga
    33961 acctgtcacg attgtttcta ccggaccgca aactaacgtt gccttgctgc tcaatagcca
    34021 tccggaactg catagcaaaa ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg
    34081 taactggacg cctgcggctg aatttaatat ttacgttgac ccggaagcgg cagaaattgt
    34141 cttccagtca gggatcccgg tggtgatggc cggtctggat gttactcata aagcgcaaat
    34201 ccacgttgaa gacaccgagc gtttccgcgc gattggcaac cctgtttcaa ccattgttgc
    34261 cgaactgctg gatttcttcc tcgaatatca taaagacgaa aaatggggct ttgtcggcgc
    34321 accgctgcat gacccatgca ccatcgcctg gctgttgaaa cccgagttgt ttaccactgt
    34381 tgagcgctgg gttggcgtgg aaacacaggg gaaatatacc cagggtatga cggttgttga
    34441 ttattattat ctgactggca ataaaccgaa tgccactgta attgtcgatg ttgatcgtca
    34501 gggctttgtt gatttactgg ctgatcgtct gaaattttac gcttaacact cgtcattgat
    34561 taagatcacc acgcgttacc tgggtggtct taatctattt atctcttttt ctttcaagat
    34621 ataaatgcaa tggataatgc agaactcgcc ataggtatcg atctcggcac taccaatagt
    34681 ttaattgccg tctggaaaga cggtgccgcg caattaattc caaataagtt cggtgaatat
    34741 ttaacaccat ccataattag catggatgaa aataatcata ttttagtcgg aaaaccggct
    34801 gtatcacggc gtacttcgca tccggataaa acggcagcgt tatttaaacg tgcaatgggc
    34861 agtaatacca actggcggtt aggcagcgac acatttaacg cgccagaact gtcctctttg
    34921 gtattacgct cattaaaaga agatgccgaa gaatttctgc aacgtccgat taaagatgtg
    34981 gtgatctccg ttccggctta tttcagcgat gaacaacgca agcatacccg tttagcagcg
    35041 gacttagccg ggttaaatgc ggtacgctta attaatgaac ccacagcagc tgcgatggcg
    35101 tatggcctgc atacccaaca aaatacccgt tcgctggtgt ttgatctcgg tggcggcacg
    35161 tttgacgtta cggtgcttga gtacgccacg ccggtgattg aagttcacgc ctccgctggc
    35221 gacaacttcc tcggtggcga agattttacc catatgttgg tcgatgacgt tttaaagcgc
    35281 gcggatgtca caaggaccac gcttaacgag agtgaactgg cagccttgta cgcctgtgtg
    35341 gaagcggcaa aatgtagcaa tcaatcgcca atgcacattc gctggtagta ccaggatgaa
    35401 acgcgggaat gcgaatttta cgagaacgaa ctggaagatt tgtggttgcc gctgctcaat
    35461 cgcttgcgag tgccgattga acaggcgttg cgcgatgcgc gtctgaagcc gagtcaaatc
    35521 gacagtctgg tgctggttgg cggcgcgtca caaatgccgc tggtgcagcg aatcgccgtg
    35581 cgtctgtttg gcaaattacc gtatcaaagt tacgatccga gcaccattgt cgcgctgggc
    35641 gcagcaatcc aggccgcctg ccgcttacgc agtgaagata ttgaagaagt gatcctcact
    35701 gatatttgcc cttactcgtt gggcgttgag gttaaccgcc agggcgtttc cggcattttc
    35761 tcgccgatta ttgaacgaaa caccactgtg cccgtgtcgc gtgtagaaac ttattcaacc
    35821 atgcacccgg aacaggattc aattacggtt aacgtctatc agggagaaaa ccacaaagtt
    35881 aaaaacaaca ttctggtgga atccttcgat gtgccgttga agaaaaccgg tgcttatcag
    35941 tcgattgata ttcgctttag ttatgatatc aacgggttgc ttgaagttga cgtgcttctg
    36001 gaagacggca gcgttaagtc cagagtgatt aaccacagcc cggtaacatt gagcgcgcag
    36061 cagattgaag agagtcggac gcggttatcc gcattgaaaa tttatccgcg cgatatgctc
    36121 atcaatcgca cctttaaagc caaactggaa gagttgtggg cgcgggcgct gggtgacgag
    36181 cgagaagaga ttggccgggt gatcaccgat tttgatgcgg cgctgcagtc aaacgatatg
    36241 gcccgcgtcg atgaggtcag gcggcgggcg agcgtttatt tagccattga gacctcataa
    36301 tcacggctgc cggatgcggc gtaaatgctg catccggcat cggcgcaaaa aacacatcgt
    36361 caactaaaca acatcgcgat tttgccgaga gcataaccta taaccagcac cgttaaactg
    36421 attttgataa tgctggtaat gctctgtggt ttttcaccct catcattttt ttcttcatgg
    36481 ttttcaggtg ttggctcatc gacatcttca tcctccggct cgagttttac tttcgtcaac
    36541 agcatccggg tgataatggc ctctaatgga tgagcatctc cgccttccag ccttgtatta
    36601 agacgggcca gcatctcttg tttcggaaac gcttcaagta ctacctgttc cgctacccga
    36661 tacaaatcca gtaacggtga atcttcttct gtctcgctac cgactctcga tttccaccac
    36721 gcccatgctt tgcgtttgaa gaataacgac tcaataaacg attgacctaa cggcgataat
    36781 tttccgctat gtaaaatttc cgcccataac agccgcgtcc gggcgctctc accaggaaca
    36841 tatttcagcg gttgaccttg cgcatctacc atgctccgcg cttcgataac catcactaat
    36901 aacgcgaaat aatccgggca atgacgcttg ctccactgca acaacaaatt atcagcttgt
    36961 gtagatgggt aactttccca gtacgcgccg agatcagcaa gcaggctttc cccctcgccg
    37021 cagtacaaac gttgtgcaca ctggtaatac cgggcatctt cgctttctgg ttcttccgct
    37081 tgccactgtt tggctacagg aatgagttcc accatacctg tttgcaccga gctgtaccag
    37141 cgcaacagtt tgcgatgtaa ctttgcatca tcaggaatga accacggtcc ttccatcgcc
    37201 aaaaacgcag tgacatagtc aggatggtaa cggcagcaac gttccagcgc gaagtaatat
    37261 tcaacagtct ggttctgtaa cgcgacggga tattccagaa ggctaaggaa atcgaaacaa
    37321 tcaccaacct gaatggcctc aagaaaagcc tccctttctt cctcatccat tccttcgctg
    37381 tcattaaacg aatgccagtt caaacgctct gccaacaaag aagcacaatt taatgacaga
    37441 tatcgcgctt ctatagcgat tgcacacagc ggccatcgta attgatcgac atcctccatg
    37501 ttccaggtat ttaattgctg aataaatttc tgccaggcgg agggctgaaa acgatcactt
    37561 tctgaatcca gtaatgtcct gaatgcacgt agtatttcat gttcagcggt ggcatctttt
    37621 tcttcatcat ctgcttcctc gacaggattt accgccagtc gcagcgcctc ttcataggcc
    37681 tggcgtaatg ctttaaaccc ctgcggatcg gtttcgggat gacacaacgg taagcgagca
    37741 agataagcct ggcggataat gtctatttgc gtcgtgcttt caatttcgag gatttgccaa
    37801 catgttttca taggtcgctc acatccttaa gcgttaccgg gcattcaggc tggacagtta
    37861 atggctgcca gggaagcgtg gcatagaaac ggtcgcttcc cgctgccagc tcttcgaaca
    37921 tccgactccc ggcgctatcg tatttgtaca gggttagcgc ctcgcgtagc gaagcgttgt
    37981 cttcgcactg agaagattgc cagtagagac gccctagtga ataggcagaa aaatagtgca
    38041 tccagctgtg atagtaatgg tgagctcgtg catagacgcg cgattgcagc cataaactct
    38101 cttcttccgt cagccagttg ttgagcacac cggcccgtga cagaaacccc attcgaacat
    38161 aatcccaggc tttgattccc ccttctccac agcacatagc ggtttgggca acgaattggg
    38221 tataaatcaa ccctctctcc gagccaccag caattaatgc tttccactct tcaggggaag
    38281 agcgttgcca tgtcagatat aagccagcta aatcattggc atgaccatca tcagtcatct
    38341 gataaatagt ctgaattaaa tcatcacgat tatttattcc ccaggatgat gagagatcga
    38401 attcttttat cccggtatac atttttggcg agtcatgcgc gggtttgcaa ctctgattaa
    38461 gtatttccat gggcgcagaa agcgcaaaaa gaagatattg ttcttcctta ttcatttttt
    38521 aataatccct taagtgcatt aactgactac ttttttatta ttgatgagta taaacttgac
    38581 gctttcaaaa taaaaagaaa atcgaagcat tcacacatga ataaaaaatt aatgtatata
    38641 ttcgcaattt ttatagttgc agcaattacc tgtattagcc aacccaagaa aacgatgttg
    38701 cgtgataaag ccatggtgaa ttatgccttt gattatttaa gatcaccagg cagtctaccg
    38761 ttaaccacgg cagccacgga gctttccgcg attcatggtc actcaacatc gcaatatcgc
    38821 cttggcgaat tttatcttca tggtagcgac ggtaaaccac tggattatac acaggcgaga
    38881 tactggtatg agcaatcagc agaacaggaa aatccgcgcg cacaaagcaa actggggtgg
    38941 atctacctga aaggcctggg agtcaaaccc gatacccgta aagcaattct ctggtataag
    39001 gaagcagctg aacaagggta tgctcatgct caatatactt tgggtttgat ctacagaaat
    39061 ggcactggta ttaatgttaa ccattatgaa tctcaaaaat ggttaaaact ggccgccaaa
    39121 caacattaca aaaatgcgga aagattactt gccggacttc ccgcacatta acgtcgactg
    39181 tcacccgaag agatggagta ttttccctat caaaccggca aagataaaaa tgtagaaaat
    39241 aattttgatg acgcccagac agcccagccc gccatcatca acgggcattg gctgcggttg
    39301 cggctccggc tcgacggttt cctgaccggt aaaattcgcc gcctgaatta ataatcgacg
    39361 aataattaat gcttccagtg gacaacgatc ttctgcatca aggcggctca caatcggcat
    39421 taatgattct tctgtcggtt ggctggcctg cactaattgt ttggcaacta aatataatgg
    39481 ccataaggga tgttctgtat cacgcgtgag tgagatccgc ggcttacgtc ctttgtgcaa
    39541 aaccatctcg accagtgaac gccccagcgc agagagtttt tcatcgctga gtatttcgta
    39601 taaatgaaaa cgcgtacggg cgctgtcgcc ggggacataa agtagcggtt ttccctgatc
    39661 gttaaccaga tcacgcgctt caatcatcat caccactaat gggtaataat agacccgatg
    39721 ctgacggcac cattgcaaca ttaaagcatc cgcctgggtg gagggatact cgcgccagta
    39781 gtaacaaagt tctgccagca ggctttcgcc ttctccacaa tagacgcgtt gtgcgtattc
    39841 ataataacgc gcatcttcat tatcaggttc agcttcccgc cacgaccggg cgtaatccag
    39901 gagttcgggg attcccaggc ggcttgcgct ataccagcgg agtaaatttc tgtgcagttt
    39961 tgcatcatcg gggataatca ctgcgcgatg ttgcgctaaa tacaatgtca gccattctgg
    40021 atgcgatgac caaatccgag cgaacatttg gtaaaaatcg atggtgtcat tttgcacggc
    40081 aactggcaga tgcagaatgg tctggaagtt aaaaacattc cccttcgcaa tggcatataa
    40141 aaaggattcc agttcttctt cgtctatttc cccgacgttt tcctcctgca accaccgcaa
    40201 tctttctgct aataacacca cgcactcgaa ggataaatgg gcagtgttca tggcgattgt
    40261 gcacagcgac cagcgtaatt catcaatctc ttccatcgag caataattta attgctgaat
    40321 gaatcgctgc caggcggagg acagaaaacg ttcgctatca gaggcaagta acgcgcgaaa
    40381 ggcgagcaga atttcatgtt ctgctacctc atattcttct ggttgccaaa cagatttagc
    40441 aggcgactgc gcaatccgta gcgcctcctc atacgcctga cgaagttgtt taaaaccctg
    40501 cggatcggtt tctggatgaa aggacggtaa cagcgccaga taagcgcggc ggataatatc
    40561 gacgtcagtc gtttcctcta tatcgaggat cttccagcaa tttttcataa gtcgctcata
    40621 tccttaagcg tttccgggca tgtggggtag tcagccagtg gttgccagga caaggtaggg
    40681 taatacgcat catcactggc aaataattga taaaacatat cattgccgga atcgtcgtat
    40741 tctttacgcg ctaagaattc gcgtaataaa ggcagatgat catcttcggg agactgccaa
    40801 tattgccgac caaaggtata accggcaaag tattgccgcc agttactata ataacgtaga
    40861 gcccgtagat gaatacgcga ctggatccat agactttctt cttcactcaa ccagttatta
    40921 agtacgccca ttcgacttaa aaaacccatc cggacataat cccatgcttt aattcctccc
    40981 tcaccacagc attcggctgt gctggcgaca aattgcgcat aagcctgacc ttgctcattt
    41041 aattcagcaa gatagtcacg ccattcacat ggagaataac gaaaccagcg gtgataaaac
    41101 cctgccaacc gagcggcatg accgttctcg atcattcggt gaattatgcg cagcaggtca
    41161 tcacggtcat caattcccca tgaggaagaa aggtcaaaaa agcatgtacc taaatacatt
    41221 ttgggggatg aatgcgaagg taaacagcat tcattgcgta tttccatcgg agtggaaagt
    41281 gcaaacaata ttttttgcga ccccatgtcc atataattta tccttaaaca ttcatttaat
    41341 gaatgtaaac tacatttcga ggctcagtta tttatacttt tctgacgcaa cataatacgc
    41401 ataaaataag gcttacgcaa aaatggaata cataaaaata taatatattt tatctacaac
    41461 cataaggaaa tgataatgat ttttacgtca agttgctgcg ataatttatc aatagatgag
    41521 attatcgaac gtgctgaaaa aggcgattgc gaggctcaat atattgttgg gttttattat
    41581 aatcgcgata gcgcaattga ctctccggac gacgaaaagg ccttttactg gctgaagctg
    41641 gccgcagagc aaggtcattg tgaagcacag tattccttag ggcaaaagta taccgaggat
    41701 aaaagccgtc ataaagataa tgagcaagcc atcttctggc tgaaaaaagc tgccctacaa
    41761 ggccatactt tcgcttccaa cgcccttggc tggatactgg atcgtggaga agaccccaac
    41821 tataaagaag cggttacctg gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa
    41881 aataatcttg gatggatgta cagaaatggc aacggagtcg cacaagacta tacgctggca
    41941 tttttctggt acaaacaagc tgcattacaa ggccatagtt acgcacaaga caatctggcc
    42001 gatctttata aagacggaga aggcgttgct caaaacaaga cactcgccgc attctggtat
    42061 ttgaaaagcg cacagcaggg taatcggcac gcccagtttc aaattgcatg ggattataac
    42121 gctggcgaag gggtggacca ggactataag caagcgatgt actggtatct gaaggctgcc
    42181 gctcaggaaa gcgtcggcgc ttacgtcaac atcggttata tgtataaaca cggacaaggc
    42241 gttgagaaag attatcaggc tgcctttgaa tggtttacga aagccgctga atgcaatgac
    42301 gccactgcct ggtataacct ggccattatg tatcactacg gagaaggaag acctgtcgat
    42361 ctccgacagg ctctcgacct gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt
    42421 caggaaattc gtgagactga agctttactg tagaaataaa aacaagggcc ggggaatttc
    42481 attctgccca tattctcttt attgctgtgc gaaatcagac tattacggct caaacgggcg
    42541 tctctggaac tggtcataac cacatttcgg gcatagcgtc agcacttccg gtgtgtagat
    42601 cgggagatgg aagtgacatt tctcgcagac cagatttccc agcccgacca cttctccgct
    42661 gtgataaacc ccatgatgat tgaggtcctg gaaaatttcg cgccattcaa gctgcgtttt
    42721 atcggtgatg tctgccagct cctgccacaa gctttcttta atcacccgca taaagacgct
    42781 gtcagattct tctttcaggc tctcttcata gctcatggcg aactcttcca ggtcacgtct
    42841 gacagctcgc gtcagctcat cgatctcggt tcgcgttaac tcccctgttt ttattacgcg
    42901 ctcgcgcgcc tgttccacca gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgtt
    42961 cagtgacgcg accagttcac ggtaatattg agcaaccttg ttcatcgttt catctcccgg
    43021 gtcgttaact gactataata atagacgttc tttgttcctt gctttgctaa tacgcccaca
    43081 aactctgtaa attaatgtac gaataatccg ggcatcattt caacgtcgag tgcgcgaaag
    43141 gctgttttga cgcggacgtt tgggctatgc tatgcggatc tgaaaaacca cctcaacgct
    43201 acatttgtag ccgtattgaa aacaggacca ctggctgcca tgcaagagca ataccgcccg
    43261 gaagagatag aatccaaagt acagcttcat tgggatgaga agcgcacatt tgaagtaacc
    43321 gaagacgaga gcaaagagaa gtattactgc ctgtctatgc ttccctatcc ttctggtcga
    43381 ctacacatgg gccacgtacg taactacacc atcggtgacg tgatcgcccg ttaccagcgc
    43441 atgctgggca aaaacgttct gcagccgatc ggctgggacg cgtttggtct gcctgcggaa
    43501 ggcgcggcgg tgaaaaacaa caccgctccg gcaccgtgga cgtacgacaa catcgcgtat
    43561 atgaaaaacc agctcaaaat gctgggcttt ggttatgact ggagccgcga gctggcaacc
    43621 tgtacgccgg aatactaccg ttgggaacag aaattcttca ccgagctgta taaaaaaggc
    43681 ctggtatata aaaagacttc tgcggtcaac tggtgcccga acgaccagac cgtactggcg
    43741 aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata ccaaagttga acgtaaagag
    43801 atcccgcagt ggtttatcaa aatcactgct tacgctgacg agctgctcaa cgatctggat
    43861 aaactggatc actggccaga caccgttaaa accatgcagc gtaactggat cggtcgttcc
    43921 gaaggcgtgg agatcacctt caacgttaac gactatgaca acacgctgac cgtttacact
    43981 acccgcccgg acacctttat gggttgtacc tacctggcgg tagctgcggg tcatccgctg
    44041 gcgcagaaag cggcggaaaa taatcctgaa ctggcggcct ttattgacga atgccgtaac
    44101 accaaagttg ccgaagctga aatggcgacg atggagaaaa aaggcgtcga tactggcttt
    44161 aaagctgttc acccattaac gggcgaagaa attcccgttt gggcagcaaa cttcgtattg
    44221 atggagtacg gcacgggcgc agttatggcg gttccgggtc acgaccagcg cgactacgag
    44281 tttgcctcta aatacggcct gaacatcaaa ccggttatcc tggcggctga cggctctgag
    44341 ccagatcttt ctcagcaagc cctgactgaa aaaggcgtgc tgttcaactc tggcgagttc
    44401 aacggtcttg accatgaagc ggccttcaac gccatcgccg ataaactgac tgcgatgggc
    44461 gttggcgagc gtaaagtgaa ctaccgcctg cgcgactggg gtgtttctcg ccagcgttac
    44521 tggggcgcgc cgattccgat ggtgacgctg gaagacggta ccgtaatgcc aaccccggac
    44581 gaccagctgc cggtgatcct gccggaagat gtggtgatgg acggcattac cagcccgatt
    44641 aaagcagatc cggagtgggc aaaaactacc gttaacggta tgccagcgct gcgtgaaacc
    44701 gacactttcg acacctttat ggagtcctcc tggtactatg cgcgctacac ttgcccagag
    44761 tacaaagaag gtatgctgga ttccgaagcg gctaactact ggctgccggt ggatatctac
    44821 attggtggta tcgaacacgc cattatgcac ctgctctact tccgcttctt ccacaaactg
    44881 atgcgtgatg caggcatggt gaactctgac gaaccagcga aacagttgct gtgtcagggt
    44941 atggtgctgg cagatgcctt ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc
    45001 ccggttgatg ctatcgttga acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg
    45061 gcaggccatg aactggttta taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt
    45121 atcgacccgc aggtgatggt tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg
    45181 tttgcttctc cggctgatat gactctcgaa tggcaggaat ccggtgtgga aggggctaac
    45241 cgcttcctga aacgtgtctg gaaactggtg tacgagcaca cagcaaaagg tgatgttgcg
    45301 gcactgaacg ttgatgcgct gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa
    45361 actatcgcta aagtgaccga tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg
    45421 gcgattatgg agctgatgaa caaactggcg aaagcaccga ccgatggcga gcaggatcgc
    45481 gctctgatgc aggaagcact gcaggccgtt gtccgtatgc tcaacccgtt caccccgcac
    45541 atctgcttca cgctgtggca ggaactcaaa ggcgaaggcg atatcgacaa cgcgccgtgg
    45601 ccggttgctg acgaaaaagc gatggtggaa gactccacgc tggtcgtggt gcaggttaac
    45661 ggtaaagtcc gtgccaaaat caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa
    45721 cgtgctggcc aggaacatct ggtagcaaaa tatcttgatg gcgttactgt acgtaaagtg
    45781 atttacgtac caggtaaact cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc
    45841 gatatctggc aacactgttg ttatctctgg cggtgttaat caccgccggg tgtggctggc
    45901 atctgcgtga caccacgcag gttccttcca ctatgaaggt catgatcctg gactcaggcg
    45961 atccgaacgg gccattaagc cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt
    46021 tgcttgataa agaaaccacg cgtaaggacg ttccatccct gcgtttgggt aaagtgagca
    46081 tcgcgaaaga taccgcatcg gtattccgta acggtcaaac agcagagtat cagatgatca
    46141 tgacggtgaa tgcgaccgtg ttgatccccg gccgtgatat ctacccgatt agcgccaaag
    46201 tcttccgttc attcttcgat aacccgcaaa tggcgttagc gaaagataac gaacaagaca
    46261 tgatcgtaaa agatatgtac gaccgtgctg ccgaacagct gattcgtaag ctgccaagca
    46321 tccgtgctgc ggatattcgt tccgacgaag aacagacgtc gaccacaacg gatactccgg
    46381 caacgcctgc acgcgtctcc accatgctgg gtaactgatg attcggttgt acccggaaca
    46441 actccgcgcg cagctcaatg aagggctgcg cgcggcgtat cttttacttg gtaacgatcc
    46501 tctgttattg caggaaagcc aggacgctgt tcgccaggta gctgcggcac aaggattcga
    46561 agaacaccac actttttcca ttgatcccaa cactgactgg aatgcgatct tttcgttatg
    46621 ccaggctatg agtctgtttg ccagtcgaca aacgctattg ctgttgttac cagaaaacgg
    46681 accgaatgcg gcgatcaatg aacaacttct cacactcacc ggacttctgc atgacgacct
    46741 gctattgatc gtccgcggta ataaattaag caaagcgcaa gaaaatgccg cctggtttac
    46801 tgctcttgcg aatcgcagcg tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc
    46861 ccgctgggtt gctgtgcgcg caaaacagct caacttagaa ctggatgacg cggcgaatca
    46921 ggtgctctgc tactgttatg aaggtaacct gctggcgctg gctcaggcac tggagcgttt
    46981 atcgctgctc tggccagacg gcaaactgac attaccgcgc gttgaacagg cggtgaatga
    47041 tgccgcgcat ttcacccctt ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg
    47101 cgcgttgcat attcttcagc aactgcgtct ggaaggcagc gagccggtta ttttgttgcg
    47161 cacattacaa cgtgaactgt tgttactggt taacctgaaa cgccagtctg cccatacgcc
    47221 actgcgtgcg ttgtttgata agcatcgggt atggcagaac cgccggggca tgatgggcga
    47281 ggcgttaaat cgcttaagtc agccgcagtt acgtcaggcc gtgcaactcc tgacacgaac
    47341 ggaactcacc ctcaaacaag attacggtca gtcagtgtgg gcagagctgg aagggttatc
    47401 tcttctgttg tgccataaac ccctggcgga cgtatttatc gacggttgat atgaaatctt
    47461 tacaggctct gtttggcggc acctttgatc cggtgcacta tggtcatcta aaacccgtgg
    47521 aaacgctggc gaatttgatt ggtctgacgc gggtcacaat catccctaat aatgttcctc
    47581 cgcatcgtcc ccagccggaa gcgaacagcg tgcagcgtaa acacatgctt gaactggcga
    47641 ttgccgacaa gccattattt actcttgatg aacgcgagct aaagcgcaat gccccctctt
    47701 acactgcgca aacactgaaa gagtggcggc aggaacaagg accggacgtg ccgctggcgt
    47761 ttattattgg tcaggattca ttgctgacct ttccgacctg gtacgaatac gaaacgatac
    47821 tcgacaatgc acatttgatc gtctgtcggc gtccaggtta cccacttgaa atggcgcaac
    47881 cgcaatacca gcaatggctg gaagatcatt tgacacataa cccggaagat cttcaccttc
    47941 agcctgccgg taaaatttat ctggctgaaa cgccgtggtt taacatctcg gcgaccatca
    48001 tccgcgaacg tttgcaaaac ggtgaatcgt gtgaggattt attgccggaa ccggtattga
    48061 cttacattaa ccaacaaggc ttgtatcgct gatacctgtc gttacggagg gcatgatgcg
    48121 actgtggtta attcgtcatg gcgaaacaca agcgaatgtc gatggtctgt acagcggtca
    48181 tgcgcccaca cccctgaccg cacgcggtat tgagcaagcg caaaatctgc atacactgct
    48241 agatgatgtt tcctttgacc tggttttatg cagtgaactg gaacgggcac agcataccgc
    48301 gcgactggtt ctcagtgacc gccagctccc cgttcatatc atccctgaac tcaacgaaat
    48361 gttttttggc gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa
    48421 ctatagcgcg tggtgcaatg actggcagca tgctatcccc acgaacggtg aaggatttca
    48481 ggcattttcg caacgtgtgg aacgctttat cgcaaggctt agtgaatatc agcactatca
    48541 gaatatttta atcgtcagcc atcagggcgt actgagtctg ttaattgctc gtttaattgg
    48601 catgcctgcc gagtctatgt ggcattttcg cgttgaccag gggtgttgga gcgccattga
    48661 tatcaaccaa aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa
    48721 cgcatgactt ttctgttttt ttacgggtaa gccgcaacga ccattgacag ccccgggcag
    48781 gctgatattc tccgcagcca gactttttcc gccagacacg actttgtaga aattgtttta
    48841 caaaaatggc gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga
    48901 ttcggcgaca attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat
    48961 ctatttgatt cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg
    49021 acaaaattga tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca
    49081 gcatcaccga ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg
    49141 ctgaccacgt tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg
    49201 aaaacagcgc cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg
    49261 aagagagccg tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac
    49321 ttgtcgccgt gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc
    49381 gtcgttttcc gaaagatatg cccttcgagc tgattgaaat tccggccggc aaacgcggca
    49441 agaatgcgga catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag
    49501 gcaaaaaccg cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag
    49561 ccgctgagct ggaacgctgg aagctggatg gtcgcgacgt aagtctactg attggcgggc
    49621 ctgaagggtt gtcgcctgcc tgtaaagcgg cggctgagca gagctggtcg ctgtcggcgc
    49681 ttaccctccc ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga
    49741 gcatcaccac caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc
    49801 agcggatgaa actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc
    49861 gccgggcgct ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc
    49921 tgtataatct gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca
    49981 ttaagctggt gcctatcgcg cccagccgcg gcattatcta cgaccgtaac ggtatccctc
    50041 tggccctcaa ccgcactatc taccagatag aaatgatgcc ggagaaagtc gataacgtgc
    50101 agcaaacgct ggacgctttg cgcagcgtgg tagatctgac cgatgacgat attgctgcat
    50161 tccgaaaaga gcgcgcacgt tcacaccgtt tcacctctat tccggtgaaa actaacctaa
    50221 ccgaagtaca agtagctcgc tttgccgtca atcagtaccg ttttccgggt gtcgaagtta
    50281 aaggctataa acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg
    50341 tgtcgaaaat caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact
    50401 atgcggcaac gcatgatatt ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc
    50461 acggtcagac cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt
    50521 taaaagaagt accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc
    50581 agcaatatat tgaaacgctg ctggcgggta gccgcgcagc tgtggttgtt accgaaccgc
    50641 gtacaggtgg ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ctgtttgttg
    50701 acggtatctc cagcaaagat tattccgcct tgctgaacga cccgaataca ccgctggtga
    50761 accgcgccac acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt
    50821 cggcattaag cgccggggtg atcacgcgca atacgacgct gtttgaccca ggctggtggc
    50881 aactaccagg gtcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga
    50941 atgtcacaag atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata
    51001 tggggatcga tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta
    51061 tcgacctggc ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct
    51121 ttaaaaaacc gtggtatcag ggtgacacca ttccggttgg tatcggccag ggttactgga
    51181 ccgcgacccc aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga
    51241 aggttccgca tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac
    51301 cgtatgaacc gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta
    51361 tgtacggtgt tgctaaccgc cctaatggta cggcgaataa atactttgcc agcgcaccgt
    51421 acaaaattgc ggcgaaatct ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct
    51481 ataatgcgca caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc
    51541 catacaacaa tccgcaagtg gctgtcgcca tgattctgga gaacggcggc gcgggtccgg
    51601 cggttggtac gctgatgcgc cagatcctcg accacattat gctgggtgat aacaacaccg
    51661 atctgcctgc ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa
    51721 tccgaataaa aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatttt
    51781 actggcattg ctggtttaca gcgccctggt tatctggagc gccagcggtc aggatattgg
    51841 catgatggag cgtaaaatcg gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc
    51901 gcaaattcct ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat
    51961 tttgctggtg gcggtagatg ctttcggagc catctctaaa ggcgctcaac gctggctgga
    52021 cctcggtatt gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt
    52081 tgcgcgcttt atcaaccgcg acgtttgccc gccatcgttg aagaatactg gcatcgcgct
    52141 ggtgctgata tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat
    52201 cctcgttgcg ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat
    52261 tggcgtcgca gtagtgctgg tagcggcgtt cattccgatt ctgtggttct tcctgatgca
    52321 tgattaccag cgccagcgcg taatgatgct cctggacccg gaatcagacc cactcggcgc
    52381 gggctatcac attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg
    52441 ctggctgcac ggcactcagt cacagcttga atttctcccc gaacgccata ctgactttat
    52501 cttcgcggta ctggcggaag agctgggatt agtgggcatt ctgattctgc tcgctctcta
    52561 cattctgctg atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg
    52621 cgtcatggct ggtggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat
    52681 ggtaagcggt attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc
    52741 ggcgctaatt gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa
    52801 aatgttgtcg aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca
    52861 tcgcggcagg aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg
    52921 taccgcagcc tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt
    52981 tcgaaccact gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa
    53041 tcgtgcagga tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac
    53101 caggcagtaa cctgacggcc tctggcgaag ctttcgatcc gacaaagctg acggcggccc
    53161 atccaacgct tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga
    53221 tcgtggtgcg cattaatgat cgcggccctt acggcaacga ccgcgttatt tcgctttctc
    53281 gcgcggcagc tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta
    53341 ttgtcgccca ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac
    53401 agacttacgc cctgcctgca cctcccgatt taagcggtgg cgcgggaaca agttcagtgt
    53461 ctggcccgca gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga
    53521 ccggcgcgcc ggtaaccagt agcggtttcc tcggcgctcc aacgacctta gcgcctagtg
    53581 tactggaagg cagcgaaccg acgcctgcgc cacagcccgt tgttacagct tcgtcgacaa
    53641 cgcctgcaac ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca
    53701 actttatggt gcaagtcggg gccgtaagcg atcaggctcg tgcgcaacag taccaacagc
    53761 aactgggaca gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga
    53821 tccagcttgg cccattcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa
    53881 ccgaagccca attacagtca tttattacta ccgcgcagta gcgtaaagca ggcatctgat
    53941 gtgtcaattt ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta
    54001 gtgcatttgc tatagtaggg cactttttta attccatcac ggatgtcgta gttcagacca
    54061 tgaataccat tttttccgct cgtatcatga agcgcctggc gctcaccacg gctctttgca
    54121 cagcctttat ctctgctgca catgccgatg acctgaatat caaaactatg atcccgggtg
    54181 taccgcagat cgatgcggag tcctacatcc tgattgacta taactccggc aaagtgctcg
    54241 ccgaacagaa cgcagatgtc cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt
    54301 acgttatcgg ccaggcaatg aaagccggta aatttaaaga aactgattta gtcactatcg
    54361 gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca
    54421 aaccgggcat gcaggttccg gtttctcagc tgatccgcgg tattaacctg caatcgggta
    54481 acgatgcttg tgtcgccatg gccgattttg ccgctggtag ccaggacgct tttgttggct
    54541 tgatgaacag ctacgttaac gcactgggcc tgaaaaatac ccacttccag acggtacatg
    54601 gtctggatgc tgatggtcag tacagctccg cgcgagatat ggcgctgatc ggccaggcat
    54661 tgatccgtga cgtaccgaat gaatactcga tctataaaga aaaagaattt acgtttaacg
    54721 gtattcgcca gctgaaccgt aacggcctgt tatgggataa cagcctgaat gtcgacggca
    54781 tcaaaaccgg acacactgac aaagcaggtt acaatcttgt tgcttctgcg actgaaggcc
    54841 agatgcgctt gatttctgcg gtgatgggtg gacgtacttt taaaggccgt gaagccgaaa
    54901 gtaaaaaact gctaacctgg ggcttccgtt tcttcgaaac cgttaaccca ctgaaagtag
    54961 gtaaagagtt cgcctctgaa ccggtttggt ttagtgattc tgatcgcgct tcgttagggg
    55021 ttgataaaga cgtgtacctg accattccgc gtggtcgcat gaaagatctg aaagccagct
    55081 atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta
    55141 tcaacttcca gcttgatggc aaaacgatcg agcaacgccc gctggttgtg ttgcaagaaa
    55201 tcccggaagg taacttcttc ggcaaaatca ttgattacat taaattaatg ttccatcact
    55261 ggtttggtta aaaatcgaac acttgaaagt gtaatttccg tccccatata ctaagcatca
    55321 gtaaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg ccggagctga
    55381 catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt acaaagttat
    55441 ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc gccatgcgcc
    55501 aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact cggtatctat
    55561 cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat
    55621 cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa
    55681 ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc cacggagatg
    55741 ccgttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct tacgagccaa
    55801 tctcccgggc tatgcatgaa ttcaccgata cccgcgataa tagtaccctt gatgaaatct
    55861 ggctggtcga gcactatccg gtattcaccc aaggtcaggc aggaaaagcg gagcacattt
    55921 taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg
    55981 ggccgggaca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc
    56041 gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa ctgggtatag
    56101 aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac
    56161 tgggtttacg aattcgacgc ggttgttcat tccacggtct ggcattaaac gtcaatatgg
    56221 atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa atggctaaaa
    56281 tatcacaatg gaaacccgaa gcgacgacta ataatattgc tccacgttta ctggaaaata
    56341 ttttagcgct actaaacaat ccggacttcg aatatattac cgcttaattc catacatcaa
    56401 tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac atctccttcg
    56461 cgtttgtata ttttctttat cacttgcaga aatgcggtgc cacgccttgc tccgtaactc
    56521 accccattga ttaacctatt gaaatagttg attatggtga gcaaaagcgt tccgttccaa
    56581 ttagacataa gattattgta attttttaaa tattattttt ccatgaataa aattggagta
    56641 agtgcgtgga tagtaataat caaattgaac cctgtttaag cagaaagtcg tcagaaggta
    56701 aaccacaaat atttacaacc ttacgaaata ttgatcttaa ccttctgact atttttgaag
    56761 ctgtatatgt acataaaggg atcgttaatg cagcgaaagt gcttaatctg accccctcgg
    56821 caatcagtca gtctattcag aaactgcgcg ttatattccc tgacccattg tttattcgca
    56881 aaggccaggg tgtcactcct accgcatttg cgatgcatct acatgagtat atcagtcagg
    56941 gccttgagtc cattcttggc gcgctggata tcgaaggaag ctatgataag caacgaacga
    57001 taactattgc gaccactccc tcggtcggag ccctggtcct tcctgtcatc tatcgggcga
    57061 ttaaaactca ctatccgcag cttttactgc gcaacccgcc catcagcgac gcggaaaacc
    57121 aactcagtca gtttcaaacc gatctcatca tcgataacat gttttgcacc aatcgtacgg
    57181 tgcaacatca tgttctgttc accgacaata tggtgttaat ttgccgcaag ggaaatccac
    57241 tactctcttt agaagatgac agagagacta tcgacaacgc tgcgcatgta cttctgttac
    57301 cggaagggca aaatttcagc ggtctgcggc agagagttca agagatgttt ccggaccggc
    57361 aaatcaattt caccagctac aacattttga caatcgctgc actggttgcc aacagtgaca
    57421 tgttagcgat tattcccagc cgtttttata acctgtttag tcgttgctgg ccgctggaaa
    57481 aactgccttt tccgtcctta aatgaggagc aaatagattt ctctatccac tacaacaaat
    57541 tcatcctgcg tgatcccatc ctgcacggag taatagatgt tatccgtaat gcattttgaa
    57601 aaagtattgt tacagccaga tcacagcgca gaacgataaa atggcacaat ccaacaacaa
    57661 ttttacattt tcgcgaccgc tttggctgct ttcaggtccg tttcaatgat atactgccag
    57721 tcgttaattc aaaaatagtt gataattaca acaatctatt gaattgaaac gctttccttc
    57781 gtaattcgca actggaacac gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt
    57841 aaataccgcg atgccgataa gatggccctt atcccggtta aaaacgtggc aacagagcgc
    57901 gaagccctgc tgcgcaagcc ggaatggatg aaaatcaagc ttccagcgga ctctacacgt
    57961 atccagggca tcaaagccgc aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc
    58021 tcctgcccta acctggcgga atgcttcaac cacggcacag caacgtttat gatcctcggc
    58081 gctatttgta cccgccgttg tccgttctgt gatgttgccc acggtcgccc ggtagctcct
    58141 gatgccaatg aaccagtgaa actggcgcag accattgccg atatggcgct gcgttatgtg
    58201 gttatcacct ccgttgaccg tgatgacctg cgcgatggcg gtgcccaaca ctttgcggat
    58261 tgtattactg ccattcggga aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat
    58321 ttccgcggtc gaatggatcg tgctctggat attctgactg caacgccccc agatgtgttc
    58381 aaccataacc tggaaaacgt accgcgtatt taccggcagg tacggcctgg cgcagattac
    58441 aactggtcgc tgaagctgct ggaacgcttt aaagaagcgc atccggaaat cccgaccaag
    58501 tctggtctga tggtggggct gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac
    58561 ctgcgccgtc atggtgtgac gatgttaacg ctggggcaat atttgcagcc aagccgccat
    58621 cacctgccgg ttcaacgtta cgttagcccg gatgagttcg acgaaatgaa agccgaagcg
    58681 ctggcgatgg gctttaccca tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc
    58741 gatttgcagg cgaaaggaat ggaagttaag taatcctgta ataattactt acatctgtct
    58801 gataaaaaac ccgctttttg aagcgggttt tttgtatcga acaagatatt gagggagcgt
    58861 cctgctcgcc acgtcactct ttatgagaga gcttttcagc ctgaagatca acgtctgcgc
    58921 cttttttcgc cgcagcatcg tcatcattca tcgccttctt gaacccttta atggccgctc
    58981 caaggtctcc gcccagcgta cgtaacttct tagtcccaaa cagcagaacg accagcgccg
    59041 caactaccag cagtttggta atactaatct cacccataga taccttcttg acatataacc
    59101 ggcaacagaa cgcgccttaa aaaccagaga atattaacgg tcattcgacg cgcgcacaca
    59161 atagcaatct gtaacaaggt gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg
    59221 cggcgcaaag cgacggttgt ttaagatggg cagttgcgcg cgcacctgac gcacacgttc
    59281 gggcgtcact tccgccataa tgagtgcagg catttctgac gctgccgcaa tggtgacgcc
    59341 aaagggatct ataatccggc tttgcccgat atttttgttc ccgcactccc ccgccgccac
    59401 catataacag gtggtatcca gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt
    59461 gagcggccca cgaacccagg cggcaggaag taccaggatt tcagctccct gtaaagcctg
    59521 tgccagcgcc agctctggaa agcgtaagtc ataacaggtc atcagaccga ccttcatccc
    59581 ttccacctcc agtaacggag ctatttcatt accagcatca acatggcgtg attcctgaat
    59641 ggcaaatgca tcatagagat gcagtttggc ataacgggcg acgatgtttc ctgcctgaag
    59701 tgccaccagc atattccatg cgcgccccgg cgttgaagga agatgaatcg tcagaattgt
    59761 cgtcatcatg ttacgtttac tttctcgccg taaacgtccg aggaattcgc cttccagcag
    59821 ctgtgctgat ttaaccgata gatctgcatc atgatcatcg cgcgccagca atgcttccgg
    59881 caggacaaac agcgatacgt cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc
    59941 agcgttcttt tcccacacag atgtaacagc aaactgtcct gcagcaacca gcattgccac
    60001 ctcctgtcag agtcgtaaaa ttcgccaacg acgattattc ggcgttacac ttatcactca
    60061 tacaaatcaa atagcaggat tttgcagtgt tacaacttct tttagcagtt tttattggcg
    60121 gtggtacggg aagcgtagcg agatggctgt taagtatgcg atttaacccg ctgcatcagg
    60181 cgattccgtt ggggacgctg gcagcaaacc tgattggggc attcatcata ggaatgggat
    60241 tcgcctggtt cagcaggatg acgaacattg atccagtgtg gaaagtatta atcaccaccg
    60301 gattttgtgg cggtctaaca accttctcaa cattttcggc agaagtggtg tttttgttac
    60361 aagagggccg ctttggctgg gcattactga acgttttcgt caaccttctg gggtcttttg
    60421 ccatgaccgc actggcattc tggctgtttt cggcctcaac cgtacactaa agaccaaaaa
    60481 aaacccgctg attaagcggg ttttgaattc ttgctgacgt atcttacaga gcgattacgt
    60541 ttgcagcaga agggcctttg gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag
    60601 caagagtttt aaaaccatta gtctggattg cagagaagtg tacgaacacg tctttgctgc
    60661 cgtcttccgg agtaatgaaa ccgaatcctt tggactcatt aaaccactta acgttacctt
    60721 taatcttaga catcaaaatt acctttacat gaaaaataga cacaaatgct gtgtcggtta
    60781 tcagtacacc aattgtggta cgctttgtcc agtgcaactt tactaaaaag tgataaaagt
    60841 cgcgttattt ttgatgttgc ttattttagt tattgatttt aaagaagtta ctaaaacttc
    60901 atttgtctca aaactgaaag cgcatccagg caaagtacac attgccattg ttgtaggtac
    60961 ccggaatgta ggtcatctga aaagtcactg ggccataacc cacggaggcc aatggcagta
    61021 gaaccgggag agggatgtaa ttccagttat cgcgtgccgt tacgccagcg gtgaatccca
    61081 gacctaaatg aaaattttca tccgccagcg gtcgccaggt actttcccat ccgtatccgg
    61141 caatcggttc ccatttgttc cacgagtcct taaatgccat ggcatacagg ccatgccagt
    61201 ttcctttttc atcccaacgc gacaggccaa aaccgccacc ccacggtcgc tcgttatagc
    61261 gatcggtttt ttctttgtcg taagcgaaac gtgcatgcca ggtgatggca ggaatatata
    61321 aatcataatg ttcaggctgt tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc
    61381 tctcatctgc gttagcaaaa actttaccaa cgctgattaa ctgaataaaa acaaaggaaa
    61441 agatagcgac atatttactc acgttcattt gtgaccataa aacatttatc aaaaatctac
    61501 tactagcata gcaaagctac aattaacata accttaatag acccaacata aagaataatc
    61561 tgaatagctg ctctatttat ttccgcttgt tcaagttaaa tatttccgcg catcgattaa
    61621 agatgatgcg tcgttttctg cgatgggaat agtcaaaaaa gaaaaaccaa gtcttttttg
    61681 atgacaaaat gcaatcaagg aaaaattatt ttattttttt aacgagatac aacaatcatc
    61741 ttaacgaagt atataatata tatcgtcaac ccatgctcat attagattat tttttgagcg
    61801 cacagccgca gctaacacca ttgcaattaa caaatttgca tcaatccacc atcaatttac
    61861 acacattatt atgtgataat tgccaaccgc taaatatgcg ttttgttatc tatgtataaa
    61921 aacagcaact tcaatgtctt aatggcagtt tttcttgatt ttaatcagca ttcatcgcca
    61981 atttattggg catatttttt ccttaagctt taggaagttt ttattattta ctttgggcct
    62041 ggagacagga aaaattatgc tgacattcat tgaactcctt attggggttg tggttattgt
    62101 gggtgtggct cgctacatca ttaaagggta ttccgccact ggtgtgttat ttgtcggtgg
    62161 tctgttattg ctgattatca gtgccattat ggggcacaaa gtgttaccgt ccagccaggc
    62221 ttcaacaggc tacagcgcca cggatatcgt tgaatacgtt aaaatattac taatgagccg
    62281 cggcggcgac ctcggcatga tgattatgat gctgtgtggc tttgccgctt acatgaccca
    62341 tatcggcgcg aatgatatgg tggtcaagct ggcgtcaaaa ccattgcagt atattaactc
    62401 cccttacctg ctgatgattg ccgcctattt tgttgcttgt ctgatgtcac tggccgtctc
    62461 ttccgcaacc ggtctgggtg ttttgctgat ggcaaccctg tttccggtga tggtaaacgt
    62521 tggtatcagt cgtggcgcag cagctgccat ttgtgcctcc ccggcggcga ttattctcgc
    62581 accgacttca ggggatgtgg tgctggcggc gcaggcttcc gaaatgtcgc tgattgactt
    62641 cgccttcaaa acgacgctgc ctatctcaat tgctgcaatt atcggcatgg cgatcgccca
    62701 cttcttctgg caacgttatc tggataaaaa agagcacatc tctcatgaaa tgttagatgt
    62761 cagtgaaatc accaccactg cccccgcgtt ttatgccatt ttgccgttca cgccgatcat
    62821 cggtgtactg atttttgacg gtaaatgggg tccgcaatta cacatcatca ctattctggt
    62881 gatttgtatg ctgattgcct ccattctgga gttcctccgc agctttaata cccagaaagt
    62941 tttctctggt ctggaagtgg cttatcgcgg gatggcagat gcgtttgcta acgtggtgat
    63001 gctgctggtt gccgctgggg tattcgctca ggggcttagc accatcggct ttattcaaag
    63061 tctgatttct atcgctacct cgtttggttc ggcgagtatc atcctgatgc tggtattggt
    63121 gattctgacc atgctggcgg cagtcacgac cggttcaggc aatgcgccgt tttatgcgtt
    63181 tgttgagatg atcccgaaac tggcgcactc ttccggcatt aacccggcgt atttgactat
    63241 cccgatgctg caggcgtcaa acctgggtcg taccctatca cccgtttctg gcgtagtcgt
    63301 tgcggttgcc gggatggcga aaatctcacc atttgaagtc gtaaaacgca cctcggtgcc
    63361 ggtgcttgtt gggctagtga ttgttatcgt tgctacagag ctgatggtgc caggaacggc
    63421 agcagcggtc acaggcaagt aaatagtaat gccggaggca agtttcctcc ggctttatca
    63481 tgtcacccac tgtggtatat gcgttgtggt ctgccaactt tgccgtgaac aatttcagca
    63541 ataatcagat ggcggctggc gcaatattca agataacgcc tggcagtggt gcggctgatg
    63601 gtcagtgcct gcgccaccgt ttctgccgta tgttgcacac caggctcttt aaacagtttt
    63661 cgcaccgcgt ttagcgtcaa ggggtcaatg ccggtcggca gttcgtcttt gggttcaccg
    63721 cgagcatagg cattaaacat ctcatcaatt tgcttctggc tggcgctatc aatactttcc
    63781 agcatatgtt tacgctggcg gaaacgggtt agcgtttgcc ccagtgtatt gattcacttg
    63841 aagtacgaaa aaaccgggag aacattggat tattcgggat ctgatgggat tagatttggt
    63901 ggggcttgca agcctgtagt acaaatttta ggcgttaacc aatgaaacgc gaaagatagt
    63961 aaaaaattgc ttttgtttca ttgaaaaaac gaaaaacaaa aacactataa atcatttcaa
    64021 tcatagcttc aaaaaacgtt caaaaccgat aacaaccagg ctgtcaccaa atgactcata
    64081 tcacaaatca gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat
    64141 gttttagctg gctcatggtt gttatacggc ttttttcgca tcttgtggtt ccaacaaagg
    64201 agctacaggc tcttcctgtt tatgaacacc atacattgac aaaatctctt ggcgagtttc
    64261 tggaggtaat ttatgcagcg ccaaagctgt ttgtagtgtt tggcgatcaa tacccagcgt
    64321 tgccaatagg gtgtcaacgg agatggaggg gcgttcttgc attaatccgg aaagcccata
    64381 tttagccatg tttagctcta tctctcggac ttcattttct cctagtttat tgattaacag
    64441 ggagaacatt ttcaatgtat cagcattcat ttcttcgata gcaatttttt gcatagccgg
    64501 tattgccggg atatattcaa ccccaggccc gcgacgccct tcttgtgaac gcttcggcca
    64561 tccttctttc tttgctcgtt tatgcacccc ctgtattgtc cctggcattc catccagttc
    64621 agtaatttgt ttgggtgtca tccactggtt tatctcattt gcgcttttca taaactaagt
    64681 ttaccagatt tatctatata aaaatcaatg gattacaaaa taaaccacat taactgatat
    64741 aaaccagttt tgtttatttg gtaatgtata aaacagataa tcaagacgaa ttatttgttt
    64801 tggtaaccct ttatcatacg caatatggtt ggtgtgcgaa agatgagtaa cgattggcat
    64861 cgggccgacg tgattgcggc cctcaaaaaa acaggaaccc ctctggcaaa attgtccaga
    64921 cgttcaggtt attcaaccgg gacgcttgca aatgcattag tcaggccatg gcctaaaggt
    64981 gaacgcatta ttgctgacgc cttaaacatg aaacctgagg aaatttggcc tagtagatat
    65041 ctaagggatc acaatgagag agtggatatc agcaattgaa tgcatgggtt tatctggttt
    65101 acctggttca ttaatggggg ttcacaaaag agctcagaga gaaggatggc tcaagagaag
    65161 aagagaagga attagagggg ttgcttacga ataccatata tcttctcttc ctaacattgc
    65221 tcgagctgaa gttttgctcc aacaaggaaa atttaaaacc agtcagggat gttttgaaat
    65281 cgcccgcccc acgctggaag ctcatgatta tgatcgtgag gcactatgga gcaaatggga
    65341 taaagccagc gattcccagc gcagcctggc tgaaaaatgg ttgccctcaa ttcaggccac
    65401 agacgaaatg ctgaaccagg ggatttcaac gaaaacggct tttgcgaccg ttgcagggca
    65461 ttaccaggtc agcgcatcca ctttgcggga caagtattac caggtacaga agtttgcgaa
    65521 gcctgactgg gcggctgcac ttgttgatgg acgtggagca tcccgtcgca atgttcacaa
    65581 aagtgaattt gacgaggatg cctggcagtt tctgattgca gattatctgc gaccggaaaa
    65641 acccgccttc cgcaaatgtt atgagcgtct ggaactggca gtccgcgagc atggctggag
    65701 tattccctcc cgtgccacgg cctttcgccg gattcagcaa ctggacgagg caatggttgt
    65761 tgcctgtcgt gaaggtgaac atgcactgat gcatctgata ccggcacagc agcgaactgt
    65821 ggaacacctg gacgccatgc agtggatcaa cggcgacggt tatctgcata acgtctttgt
    65881 acgctggttt aacggtgatg tgatccgtcc gaaaacatgg ttctggcagg atgtgaaaac
    65941 ccgaaaaatt ctgggctggc gctgcgatgt gagcgagaac attgattcaa ttcgcctctc
    66001 gttcatggat gttgtgactc gctacagtat cccggaggat tttcacatca ccattgataa
    66061 cacccgtggt gctgcgaata aatggctgac gggaggcgcg cccaatcgct accgctttaa
    66121 ggtaaaagag gacgatccaa aaggactgtt tttactgatg ggtgcgaaaa tgcactggac
    66181 aagcgttgtt gccggtaaag gctggggcca ggcaaaacct gttgaacgtg ctttcggtgt
    66241 tggtgggctt gaggaatacg ttgataagca tccggcactg gctggcgcat atacggggcc
    66301 aaatccgcag gcaaaacctg ataactatgg cgaccacgct gttgatgcag agctgtttct
    66361 gaaaaccttt gccgaaggtg tggcgatgtt caatgccaga acaggccgtg aaacagaaat
    66421 gtgcgggggc aaactctcgt ttgatgatgt tttcgagcgt gaatacgcca gaacgattgt
    66481 gcgtaagcca accgaagaac aaaaacggat gctgttactg cctgccgagg cggtgaacgt
    66541 ttcacgcaaa ggcgagttta cgcttaaagt tggcggctcc cttaaaggcg cgaaaaacgt
    66601 ttattacaac atggcattaa tgaatgccgg agtgaaaaaa gttgtggtca gatttgatcc
    66661 acagcagtta cactcaggct acctcagttc tggataaaat tttgaactat accaacaccg
    66721 aaatgaaaga agcgatagcg cagcgtaaac cagtggttac caccgctggg ccgcgtagtc
    66781 tgattttccg tggggccatt accggtgtag ataccagcaa agaaggg
//
LOCUS       DAJTRB010000007        65639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTRB010000007 DAJTRB010000000
VERSION     DAJTRB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.007"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(79..558)
                     /gene="folK"
                     /locus_tag="OO330_000521"
     CDS             complement(79..558)
                     /gene="folK"
                     /locus_tag="OO330_000521"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5881517.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(555..1973)
                     /gene="pcnB"
                     /locus_tag="OO330_000522"
     CDS             complement(555..1973)
                     /gene="pcnB"
                     /locus_tag="OO330_000522"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5881518.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2012..2938)
                     /gene="gluQRS"
                     /locus_tag="OO330_000523"
     CDS             complement(2012..2938)
                     /gene="gluQRS"
                     /locus_tag="OO330_000523"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5881519.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2975..3430)
                     /gene="dksA"
                     /locus_tag="OO330_000524"
     CDS             complement(2975..3430)
                     /gene="dksA"
                     /locus_tag="OO330_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5881520.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3608..4312)
                     /gene="sfsA"
                     /locus_tag="OO330_000525"
     CDS             complement(3608..4312)
                     /gene="sfsA"
                     /locus_tag="OO330_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5881521.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4327..4857)
                     /gene="thpR"
                     /locus_tag="OO330_000526"
     CDS             complement(4327..4857)
                     /gene="thpR"
                     /locus_tag="OO330_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5881522.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4931..7360
                     /gene="hrpB"
                     /locus_tag="OO330_000527"
     CDS             4931..7360
                     /gene="hrpB"
                     /locus_tag="OO330_000527"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5881523.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7556..10090
                     /gene="mrcB"
                     /locus_tag="OO330_000528"
     CDS             7556..10090
                     /gene="mrcB"
                     /locus_tag="OO330_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5881524.1"
                     /translation="MAGNDREPIGRKGKLTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10310..12553
                     /gene="fhuA"
                     /locus_tag="OO330_000529"
     CDS             10310..12553
                     /gene="fhuA"
                     /locus_tag="OO330_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5881525.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12604..13401
                     /gene="fhuC"
                     /locus_tag="OO330_000530"
     CDS             12604..13401
                     /gene="fhuC"
                     /locus_tag="OO330_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5881526.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13401..14291
                     /gene="fhuD"
                     /locus_tag="OO330_000531"
     CDS             13401..14291
                     /gene="fhuD"
                     /locus_tag="OO330_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5881527.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELMTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14288..16270
                     /gene="fhuB"
                     /locus_tag="OO330_000532"
     CDS             14288..16270
                     /gene="fhuB"
                     /locus_tag="OO330_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5881528.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16305..17585)
                     /gene="hemL"
                     /locus_tag="OO330_000533"
     CDS             complement(16305..17585)
                     /gene="hemL"
                     /locus_tag="OO330_000533"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5881529.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17810..19231
                     /gene="clcA"
                     /locus_tag="OO330_000534"
     CDS             17810..19231
                     /gene="clcA"
                     /locus_tag="OO330_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5881530.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19255..19320
                     /locus_tag="OO330_000535"
     CDS             19255..19320
                     /locus_tag="OO330_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881531.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19313..19657
                     /gene="erpA"
                     /locus_tag="OO330_000536"
     CDS             19313..19657
                     /gene="erpA"
                     /locus_tag="OO330_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5881532.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19704..20327)
                     /locus_tag="OO330_000537"
     CDS             complement(19704..20327)
                     /locus_tag="OO330_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5881533.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGEIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20365..21165)
                     /gene="btuF"
                     /locus_tag="OO330_000538"
     CDS             complement(20365..21165)
                     /gene="btuF"
                     /locus_tag="OO330_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5881534.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21158..21856)
                     /gene="mtnN"
                     /locus_tag="OO330_000539"
     CDS             complement(21158..21856)
                     /gene="mtnN"
                     /locus_tag="OO330_000539"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5881535.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21940..23457
                     /gene="dgt"
                     /locus_tag="OO330_000540"
     CDS             21940..23457
                     /gene="dgt"
                     /locus_tag="OO330_000540"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5881536.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23587..25011
                     /gene="degP"
                     /locus_tag="OO330_000541"
     CDS             23587..25011
                     /gene="degP"
                     /locus_tag="OO330_000541"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5881537.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25166..26322
                     /gene="cdaR"
                     /locus_tag="OO330_000542"
                     /pseudo
     CDS             25166..26322
                     /gene="cdaR"
                     /locus_tag="OO330_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26411..26797)
                     /locus_tag="OO330_000543"
     CDS             complement(26411..26797)
                     /locus_tag="OO330_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5881538.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27111..27935)
                     /gene="dapD"
                     /locus_tag="OO330_000544"
     CDS             complement(27111..27935)
                     /gene="dapD"
                     /locus_tag="OO330_000544"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5881539.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27966..30638)
                     /gene="glnD"
                     /locus_tag="OO330_000545"
     CDS             complement(27966..30638)
                     /gene="glnD"
                     /locus_tag="OO330_000545"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5881540.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVHQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30700..31494)
                     /gene="map"
                     /locus_tag="OO330_000546"
     CDS             complement(30700..31494)
                     /gene="map"
                     /locus_tag="OO330_000546"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5881541.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31862..32587
                     /gene="rpsB"
                     /locus_tag="OO330_000547"
     CDS             31862..32587
                     /gene="rpsB"
                     /locus_tag="OO330_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5881542.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32845..33696
                     /gene="tsf"
                     /locus_tag="OO330_000548"
     CDS             32845..33696
                     /gene="tsf"
                     /locus_tag="OO330_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5881543.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33843..34568
                     /gene="pyrH"
                     /locus_tag="OO330_000549"
     CDS             33843..34568
                     /gene="pyrH"
                     /locus_tag="OO330_000549"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5881544.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34860..35417
                     /gene="frr"
                     /locus_tag="OO330_000550"
     CDS             34860..35417
                     /gene="frr"
                     /locus_tag="OO330_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5881545.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35509..36705
                     /gene="ispC"
                     /locus_tag="OO330_000551"
     CDS             35509..36705
                     /gene="ispC"
                     /locus_tag="OO330_000551"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5881546.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36894..37652
                     /gene="ispU"
                     /locus_tag="OO330_000552"
                     /gene_synonym="uppS"
     CDS             36894..37652
                     /gene="ispU"
                     /locus_tag="OO330_000552"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5881547.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37665..38522
                     /gene="cdsA"
                     /locus_tag="OO330_000553"
     CDS             37665..38522
                     /gene="cdsA"
                     /locus_tag="OO330_000553"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5881548.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38534..39886
                     /gene="rseP"
                     /locus_tag="OO330_000554"
     CDS             38534..39886
                     /gene="rseP"
                     /locus_tag="OO330_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5881549.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39916..42348
                     /gene="bamA"
                     /locus_tag="OO330_000555"
     CDS             39916..42348
                     /gene="bamA"
                     /locus_tag="OO330_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5881550.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42470..42955
                     /gene="skp"
                     /locus_tag="OO330_000556"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42470..42955
                     /gene="skp"
                     /locus_tag="OO330_000556"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5881551.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42959..43984
                     /gene="lpxD"
                     /locus_tag="OO330_000557"
     CDS             42959..43984
                     /gene="lpxD"
                     /locus_tag="OO330_000557"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5881552.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQSNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44089..44544
                     /gene="fabZ"
                     /locus_tag="OO330_000558"
     CDS             44089..44544
                     /gene="fabZ"
                     /locus_tag="OO330_000558"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5881553.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44548..45336
                     /gene="lpxA"
                     /locus_tag="OO330_000559"
     CDS             44548..45336
                     /gene="lpxA"
                     /locus_tag="OO330_000559"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5881554.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45336..46484
                     /gene="lpxB"
                     /locus_tag="OO330_000560"
     CDS             45336..46484
                     /gene="lpxB"
                     /locus_tag="OO330_000560"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5881555.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46481..47077
                     /gene="rnhB"
                     /locus_tag="OO330_000561"
     CDS             46481..47077
                     /gene="rnhB"
                     /locus_tag="OO330_000561"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5881556.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47114..50596
                     /gene="dnaE"
                     /locus_tag="OO330_000562"
     CDS             47114..50596
                     /gene="dnaE"
                     /locus_tag="OO330_000562"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5881557.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50609..51568
                     /gene="accA"
                     /locus_tag="OO330_000563"
     CDS             50609..51568
                     /gene="accA"
                     /locus_tag="OO330_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5881558.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51667..53808
                     /gene="ldcC"
                     /locus_tag="OO330_000564"
     CDS             51667..53808
                     /gene="ldcC"
                     /locus_tag="OO330_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5881559.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAASSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYHVRVLKMAG"
     gene            53865..54254
                     /locus_tag="OO330_000565"
     CDS             53865..54254
                     /locus_tag="OO330_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5881560.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54319..55617
                     /gene="tilS"
                     /locus_tag="OO330_000566"
     CDS             54319..55617
                     /gene="tilS"
                     /locus_tag="OO330_000566"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5881561.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55666..55926)
                     /gene="rof"
                     /locus_tag="OO330_000567"
     CDS             complement(55666..55926)
                     /gene="rof"
                     /locus_tag="OO330_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5881562.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55913..56113)
                     /locus_tag="OO330_000568"
     CDS             complement(55913..56113)
                     /locus_tag="OO330_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5881563.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56279..56824
                     /locus_tag="OO330_000569"
     CDS             56279..56824
                     /locus_tag="OO330_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5881564.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56821..57243
                     /gene="arfB"
                     /locus_tag="OO330_000570"
     CDS             56821..57243
                     /gene="arfB"
                     /locus_tag="OO330_000570"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5881565.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57257..57967
                     /gene="nlpE"
                     /locus_tag="OO330_000571"
                     /gene_synonym="cutF"
     CDS             57257..57967
                     /gene="nlpE"
                     /locus_tag="OO330_000571"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5881566.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58122..58946)
                     /locus_tag="OO330_000572"
     CDS             complement(58122..58946)
                     /locus_tag="OO330_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5881567.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58999..60717)
                     /gene="proS"
                     /locus_tag="OO330_000573"
     CDS             complement(58999..60717)
                     /gene="proS"
                     /locus_tag="OO330_000573"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5881568.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60828..61535)
                     /gene="tsaA"
                     /locus_tag="OO330_000574"
     CDS             complement(60828..61535)
                     /gene="tsaA"
                     /locus_tag="OO330_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5881569.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61532..61936)
                     /gene="rcsF"
                     /locus_tag="OO330_000575"
     CDS             complement(61532..61936)
                     /gene="rcsF"
                     /locus_tag="OO330_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5881570.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62054..62869)
                     /gene="metQ"
                     /locus_tag="OO330_000576"
     CDS             complement(62054..62869)
                     /gene="metQ"
                     /locus_tag="OO330_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5881571.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LIAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62909..63562)
                     /gene="metI"
                     /locus_tag="OO330_000577"
     CDS             complement(62909..63562)
                     /gene="metI"
                     /locus_tag="OO330_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5881572.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63555..64586)
                     /gene="metN"
                     /locus_tag="OO330_000578"
     CDS             complement(63555..64586)
                     /gene="metN"
                     /locus_tag="OO330_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5881573.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64774..65349
                     /gene="gmhB"
                     /locus_tag="OO330_000579"
     CDS             64774..65349
                     /gene="gmhB"
                     /locus_tag="OO330_000579"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5881574.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15514 a        16270 c        18093 g        15762 t
ORIGIN      
        1 gtttaaacta aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat
       61 attcgtaata taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac
      121 ttcacgcaac gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca
      181 cagcataaat ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat
      241 cacttcatta ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc
      301 tttacgaaca cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg
      361 ggcgggcgaa gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc
      421 cagcggtggg gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc
      481 taatgctttc agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc
      541 aatatacgcc actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga
      601 gtacgacgac gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt
      661 ggtgcggaaa cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt
      721 tcaacttcag ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc
      781 agtttccatg cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg
      841 cgggttaacg tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg
      901 tcgttcatcg ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc
      961 tgtgccgtct ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg
     1021 cgcatatcgt tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc
     1081 atcgggctgt cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac
     1141 agatgatatt cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta
     1201 agcgattctt caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg
     1261 atcggttctg cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg
     1321 cgcagcatac gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata
     1381 acgccgtcct tcagatcctt cataccacca acgtaatcac ggacggtaaa atccgctacg
     1441 ctgtaataca ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg
     1501 aaaatgttgt cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg
     1561 acgttacctt cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt
     1621 acatgagcca gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc
     1681 tgctcaggcg tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg
     1741 tcgcgcacgc cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt
     1801 accttcaggg cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc
     1861 accgtcacct gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc
     1921 ttgcggcaaa aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat
     1981 tcaggacaaa aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat
     2041 ttacaattgc cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga
     2101 tttgctcgac gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg
     2161 ccgcgattag taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg
     2221 aaagtttagc gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc
     2281 agccaaaaag ctggtacagc gggatttgcc ttactgttgg ttcaatcaga tcagccccac
     2341 gcactatttc tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga
     2401 acaacccatc acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa
     2461 taatgcctcg cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg
     2521 cggcgttgtc tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc
     2581 tttgaatacg cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg
     2641 cgagtgcttc acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt
     2701 gcagaccgta atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag
     2761 gcgggtcgat atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat
     2821 agctgccgag cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga
     2881 agcggccaat atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt
     2941 cccgcctgtg gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa
     3001 tttcagccag cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc
     3061 gaataccaat ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt
     3121 tcagcgtctt ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga
     3181 ggctgaactc ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat
     3241 cctgcatatg tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca
     3301 gaatacgacg gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct
     3361 gatatggttc caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc
     3421 cttcttgcat gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat
     3481 gaggccgcta taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga
     3541 caactgtgtg aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact
     3601 tactttacta caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt
     3661 ccgctttgta agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt
     3721 atttctcatc gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga
     3781 aaaaaataac cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct
     3841 gacctcgttc agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg
     3901 atttcacttc aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc
     3961 tgcgttcgga gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa
     4021 ttgattcatt aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa
     4081 taaatgcgcc gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt
     4141 tgtctgaagt cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg
     4201 tattcgggca gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac
     4261 gtttgtaacg ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa
     4321 tccttgttat tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc
     4381 aaacgaggag gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg
     4441 cgggaatgtc accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg
     4501 attgctttga aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat
     4561 taagccgcgt ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc
     4621 ggcgtcatca agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag
     4681 cgccttctct ttctctgcgc tcacttcgcc taaaaatgcc agagtcaggt gcaaattatc
     4741 ggcggcgacc ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg
     4801 ttcgcggatt tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg
     4861 aggcactcgg ttataaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct
     4921 ggagcgtttt gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct
     4981 cgattgtgcg ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct
     5041 gccgctgcaa ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg
     5101 tcgtctggcg gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg
     5161 cgataccgtt ggctaccgga tgcgtgcgca aaactgcgtc gggctgaata cccgcctgga
     5221 agtggttacc gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt
     5281 tggattggtg atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc
     5341 gctgttactc gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc
     5401 tacgctggac aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga
     5461 agggcgctcg tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga
     5521 cgaagccgtt gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt
     5581 atttttacct ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg
     5641 cagtgatgtg gtgctctgcc cgctgtatgg cgtgttgtca ctgaacgatc agcgaaaagc
     5701 gatcctcccg gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac
     5761 cagtttaacc atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg
     5821 ctttgatccg cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat
     5881 gacgcagcgt gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc
     5941 caaagaacaa gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct
     6001 ttccggtttg ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg
     6061 gctggatcaa ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg
     6121 ggcgctggag ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga
     6181 tccgcgttta gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc
     6241 aaaaattgcc gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc
     6301 gttttcgcgc aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa
     6361 cgtacgcggc ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc
     6421 cgatcgcatt gctcatcgcc gtgggcaaga tggacgctat caactggcaa acagcatggg
     6481 agcgatgctc gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt
     6541 attgcagggc agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga
     6601 tgaattagta caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga
     6661 cgcgcaaggt acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt
     6721 gcagccgctg gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg
     6781 tgataaaggt ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt
     6841 gttatgcgcc gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt
     6901 gttggcgacg ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg
     6961 cctgaaatca ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca
     7021 acgtctggat agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat
     7081 tcgttatcat gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga
     7141 ggccactaat ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc
     7201 tgcccaaagg ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg
     7261 tgaggtgcaa aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc
     7321 aaacacggca ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc
     7381 ctgtgggaga gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc
     7441 tccctcaagg gagaggggac cgatcgagcg caaattttga gagatatctt cttctgtctt
     7501 gtaacagaag aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc
     7561 cgggaatgac cgcgagccaa ttggacgcaa agggaaactg acgcgtccgg tcaaacaaaa
     7621 ggtaagccgt cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga
     7681 tgaagaaccg atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa
     7741 acgcggctgg ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat
     7801 ttacggcgtt tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact
     7861 gcccgcggca gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa
     7921 cgaaatggtg aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc
     7981 tggcgaattt accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc
     8041 ggacagtaaa gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac
     8101 catcgtcaat atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat
     8161 caccatgatc tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc
     8221 tgatttgctg gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg
     8281 aatcagtctc tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt
     8341 gcagggtgcg agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg
     8401 ttcttactgg cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag
     8461 caaagaccgt attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa
     8521 cgaaatccgc ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct
     8581 aagcctcgac cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc
     8641 gtggcgtaac ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca
     8701 gcaacagatt attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca
     8761 gccgcgtggt ggggtgatct ctcctcagcc agcctttatg cagctggtgc gtcaggagtt
     8821 gcaggcaaaa ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt
     8881 tgactcggtg gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa
     8941 gaaacagcgt aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg
     9001 tgaagttcgt gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat
     9061 gcaggcgcgt cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag
     9121 ccagccgaaa atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca
     9181 gccgaatggc caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag
     9241 agtgatgctg gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat
     9301 ggcgctgggg ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca
     9361 gttgcatccg gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc
     9421 gcaggcattc cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc
     9481 ggtaatcgcg gaagatggta aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt
     9541 tccggcgcag gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac
     9601 gggtcgtcag cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac
     9661 caacaataac gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg
     9721 ggtaggccgt gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat
     9781 ttatcagcgt tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga
     9841 tattgcagat atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt
     9901 cttgccggtc tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca
     9961 gccgtcaggc aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc
    10021 tgctcagcaa gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg
    10081 tagtaattaa catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca
    10141 tcccatctaa gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg
    10201 ctcagattgc gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa
    10261 taatcattct cgtttacgtt attattcact ttacatcaga gatataccaa tggcgcgttc
    10321 caaaactgct cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt
    10381 tagcggcatg tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt
    10441 taccgctgca cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg
    10501 gcagtcagct accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc
    10561 tgttgtgacc gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag
    10621 ctacacgccg ggtgtctctg ttggtacgcg tggcgcatcc aacacctatg accacctgat
    10681 cattcgcggt tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt
    10741 gcagggcaac ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat
    10801 tatgcgtggc ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat
    10861 ggtcagcaag cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga
    10921 cagcctgttc cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc
    10981 ttatcgcctg accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca
    11041 gcgttatgct attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt
    11101 cctttcttac ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg
    11161 aaccgttgag ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa
    11221 gaacaacacc tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa
    11281 cgacaccttt actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag
    11341 cgtttatggt tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc
    11401 attagcgcca gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa
    11461 gctgcaaaac ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga
    11521 ccacaccctg ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt
    11581 tggttacgac gactctgtgc cactgctcaa tctgtacaat ccggtgaatc ccgatttcga
    11641 cttcaatgcc aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca
    11701 aacgggcgtt tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg
    11761 tcgttatgac tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg
    11821 tgatgacaaa cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac
    11881 accttacttc agctatagcg aatcgtttga accttcttcg caagttggga aggatggtaa
    11941 tattttcgca ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga
    12001 tcgtccgatt gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc
    12061 ggaccctgag ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga
    12121 aatcgaagcg aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac
    12181 cgatgcggaa tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa
    12241 acacatggct tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac
    12301 gctgggcacc ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa
    12361 agtgggaagt tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat
    12421 ggcgggctcc aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag
    12481 ctgctttaac acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac
    12541 ctttcgtttc taatttctct tttggggcac ggatttccgt gcccatttca caagttggct
    12601 gttatgcagg aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt
    12661 cgtgtgcccg ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg
    12721 accggtctga ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt
    12781 catcagcctc cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc
    12841 agcaaagcgt ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg
    12901 atgaccgtgc gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc
    12961 tttggtgcgg cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg
    13021 ctggcgcatc ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc
    13081 atgctggtgg cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc agcgctggat
    13141 atcgcccacc aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg
    13201 acggtcattg ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc
    13261 gccctgcgca gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa
    13321 accctcgaaa tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca
    13381 cctgtgagtt ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa
    13441 cggcgatggc gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg
    13501 accccaatcg tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca
    13561 tcgtgcctta cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat
    13621 tgccggactc agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgatgaccg
    13681 aaatgaaacc gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg
    13741 cacgtattgc gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg
    13801 cgcgtaaatc gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc
    13861 atttagcgca atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg
    13921 cacgcccgtt actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa
    13981 acagcttgtt ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa
    14041 ccaacttctg gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg
    14101 atgtgctctg ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc
    14161 tgtggcaggc catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt
    14221 tttatggtgc aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag
    14281 gtaaagcgtg agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat
    14341 tgtcgctacg gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc
    14401 gcaggccgcc tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt
    14461 gttgccgcgt ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct
    14521 gtttcagcaa gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg
    14581 cgcgcaactg gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca
    14641 gtttgctgcg ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg
    14701 gaaacggctg tcgccggtaa cgctgatttt cgcggggttg gtagtgagcc tttattgcgg
    14761 cgcaatcaat cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg
    14821 gagcactgga acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt
    14881 gctgggcggt gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga
    14941 tgatggcgtg gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc
    15001 gctggcgatt gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg
    15061 gttgttcgcg ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat
    15121 gctggcgtcg ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct
    15181 gactcgcgtg tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct
    15241 gctactgtgg ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga
    15301 tcgtgtcgcg actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt
    15361 gttgatggct gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc
    15421 gagcggggcg ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct
    15481 gtttgcgggc gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc
    15541 gatggcaagc ccggaagtgc tggggattag ctccggcgcg gcgtttggtg tggtgttaat
    15601 gctgtttctg gtaccgggta atgcctttgg ctggttgtta cccgcaggca gtctcggggc
    15661 ggcggtgacg ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg
    15721 catgttactg gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca
    15781 ggcaagtggt gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa
    15841 cgcgaccgat gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac
    15901 tccgctgtgc cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt
    15961 agggatggcg ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc
    16021 gaccgcgacg atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc
    16081 gcggatgatg ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg
    16141 tttactgctg gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc
    16201 ggcggggctg ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca
    16261 gagccgttaa tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac
    16321 acccgacgtg cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata
    16381 aagcccgctt caaacgctga cggtgccagg taaacacctt catccagcat catatggaag
    16441 aaacgcttaa aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg
    16501 gcgtcggtaa agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc
    16561 gcttcttctg ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt
    16621 tcgtgaatgc ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc
    16681 gggttaccgg aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt
    16741 acatcacgac gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg
    16801 caggttaaat ctggcactac ggcgaaataa tcctgtgcgc cagctagcgc tacgcggaaa
    16861 ccggtcatca cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga
    16921 cctggcagga actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg
    16981 ataatacagg caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg
    17041 ttataagtac aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc
    17101 tggcctaacg tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg
    17161 taacaacctt caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg
    17221 gcgctcatcg tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc
    17281 agttcggtca ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa
    17341 ccacgctcgg cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc
    17401 atcggccccc aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa
    17461 gcgccgtccg ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca
    17521 ggggagttca caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta
    17581 ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct gctattccat gttattcata
    17641 acaagttaaa tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag
    17701 agtataaaag ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg
    17761 cgagtaaaat gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga
    17821 tactccctct ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca
    17881 acttcttgag cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac
    17941 gcttgtcggg ctggctgcgg ttgcttttga caaaggtgtc gcttggttgc agaaccaacg
    18001 tatgggggcg ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct
    18061 ctgttcggcg gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc
    18121 agggggatcg gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg
    18181 gtggcgtgta ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggg
    18241 gctagggcgc gaaggaccaa ccgtgcagat cggcggcaat atcagtcgca tggtgcttga
    18301 tattttccgc ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc
    18361 ggggctggct gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat
    18421 gcgtccgcag tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat
    18481 gtcgaccatt atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact
    18541 ttctgatgcg ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat
    18601 tttcggccct atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca
    18661 cggcggcaat attaccaaat gggtactgat ggggggtgcg attggcggtc tgtgtggatt
    18721 gctggggttt gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac
    18781 cgcggggaat ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac
    18841 cttactctgc ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg
    18901 tactgtgctg ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct
    18961 ggaggcgggg acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc
    19021 gccgttaacg gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc
    19081 aatgattatt accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct
    19141 atactcggcg attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag
    19201 caaggccgca tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg
    19261 attattattg ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga
    19321 tgacgtagca ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat
    19381 cgctgacgaa gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag
    19441 cggcttccag tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga
    19501 aaaacagggc gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc
    19561 cgttgactac accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa
    19621 aagcacctgc ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa
    19681 actatccggc acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc
    19741 ttaagatgcc aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata
    19801 ctggctgttt ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg
    19861 ataatacagg cagttgcgta gatttctgta cgtaaaatca tggggatttc gcggaccagt
    19921 acatcacgaa tgatctcgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac
    19981 ggaccggctt ccgcattaaa ggctttattc acgccaatgc cgacaaacac cgccagacca
    20041 acggcgtcca gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg
    20101 atggtcagca tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat
    20161 accgggccgt gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg
    20221 cccagtacca gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccgtag
    20281 atggcaaata ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca
    20341 ctgagcacct gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg
    20401 tgctgcgagg ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac
    20461 gggaattttg agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc
    20521 tgtaatgaca atagcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca
    20581 gggaacccgg ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg
    20641 aatcgactct tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt
    20701 tttaggttta tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc
    20761 ggcttgttcg gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc
    20821 aatttgttca atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg
    20881 gtcaacctgc cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc
    20941 gacaatgcgt tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg
    21001 tggaggatag tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag
    21061 ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc
    21121 gagaaaagac agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca
    21181 ccagtgactc aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc
    21241 taagatgaga ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt
    21301 tgaaattgtg gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg
    21361 ggaagttgtg gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa
    21421 caatcaggcc acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga
    21481 tcagtttatc gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac
    21541 caaatgccgt gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca
    21601 ctttcagcgt tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc
    21661 agtgttccaa cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca
    21721 gaagcgcaac ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga
    21781 tagtttgacg gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa
    21841 tgatgccgat tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat
    21901 gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga
    21961 aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag
    22021 atcctgcgga tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt
    22081 ctgcaacaaa agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt
    22141 acccactcga tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt
    22201 ctgaaagagc ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa
    22261 agcattgttg agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt
    22321 ggcgaagcag cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc
    22381 cagcctctga ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa
    22441 ccgcttaacg agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca
    22501 caaggcattc gtctggtgca tacattgatg cggatgaatc tcacttgggc acaggttggc
    22561 ggtattttaa aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac
    22621 tatttaatga aaaagccggg ttattatctt tctgaagaag cctatattgc ccgcttgcgt
    22681 aaagaactta atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc
    22741 gacgacatct cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc
    22801 gttgagcagc tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc
    22861 ttttcgctgg tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt
    22921 acggaagatc agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac
    22981 gcggcacaac gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca
    23041 ttattggaag atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta
    23101 aaacatgtgt ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt
    23161 agcggattat tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa
    23221 ctggtagaaa aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc
    23281 tcgacgcgcc atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct
    23341 gagtttccgc tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt
    23401 atgaccgacc tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca
    23461 ggcttttgta aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt
    23521 cgggctataa aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg
    23581 aaatacatga aaaaaaccac attagcactg agtgcactgg ctctgagttt aggtttggcg
    23641 ttatctccgc tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg
    23701 ccaagccttg caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa
    23761 ggtagcacaa ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat
    23821 gattctccgt tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc
    23881 cagggcggta atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc
    23941 attgatgccg ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg
    24001 attaaagtcc agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg
    24061 cgctctgata tcgcgctgat ccaaatccag aacccgaaaa aactgaccgc aattaagatg
    24121 gcggattctg atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt
    24181 ctgggcgaga cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct
    24241 gaaaactacg aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt
    24301 gcgctggtta acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac
    24361 ggcggcaaca tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg
    24421 cagatggtgg aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg
    24481 aattccgaac tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag
    24541 gttctgccta attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca
    24601 ctgaacggta agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg
    24661 gtgggcagca aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg
    24721 gaactgcagc agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa
    24781 ggcgctgaga tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg
    24841 ggcactccgg ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag
    24901 caggcagtga aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg
    24961 gcactgaaca ttcagcgcgg cgacagcacc atctatctgt taatgcagta atctccctca
    25021 accccttcct gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc
    25081 atacatcttc atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg
    25141 ttaagccgtg cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca
    25201 ggatatcgtg gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg
    25261 cgggcgaatt atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt
    25321 gctggtactt tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca
    25381 cggtgtgcgg caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat
    25441 tggcctgaca ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc
    25501 tgaaatgatg ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg
    25561 ggaagaactg gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg
    25621 gcgcaacggc tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac
    25681 agcggtcagc ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg
    25741 actacgcccg agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg
    25801 aaaccggcgt tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa
    25861 caactgatta cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac
    25921 tattttaccg gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg
    25981 gtgggtaaac agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct
    26041 gtgttactcg acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg
    26101 cggctgaaag cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc
    26161 cacaatgtgc aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag
    26221 tatcggctta atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg
    26281 ttgctgctgt atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga
    26341 ggcggcgctg gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt
    26401 caattcggga ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat
    26461 tggttacgac tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt
    26521 cgctgcggtc acgctggcag atttgatcaa gctcatcaat gatataccgt agattcgggc
    26581 tgatttcctg gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct
    26641 gacgcggata tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat
    26701 agattttcag aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag
    26761 gattggtaat acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag
    26821 taaattgtgc ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa
    26881 gcctttttct tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt
    26941 ggcgatattc catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt
    27001 ccgaaacaag atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat
    27061 tctgagccag aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg
    27121 gtacgcagca gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg
    27181 ataaccgcac agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc
    27241 acagaccccg ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg
    27301 gtgctctgac caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct
    27361 tcaaccactt cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc
    27421 tgcagcggct ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca
    27481 atctgcgcac aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat
    27541 gcgccgatgt taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga
    27601 cgtaccgccg ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag
    27661 tcggcgaatt tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga
    27721 ttatcattaa tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac
    27781 tgaccgtcaa ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga
    27841 tttaccgctt cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca
    27901 aaagcggttt caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa
    27961 acacatcacc ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc
    28021 tgcaactcgt tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact
    28081 cgctcgccaa tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt
    28141 ttcccgactc gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg
    28201 cggtcggtat gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg
    28261 gcgggttggc gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga
    28321 ccaaaccgaa taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg
    28381 ataaaggtat ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt
    28441 aaattgcggc ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc
    28501 cagataaaaa tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg
    28561 cttaaatcat gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg
    28621 acaaaatagt tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta
    28681 tccatgcgca gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc
    28741 ggcgtgtttt gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac
    28801 agactttgct tccagctatt ccacagtgtt tcgttggtgg cgcaaatgtc agccactgtc
    28861 aggcatacca gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg
    28921 acttcagggt cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggtga
    28981 accagccagg caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc
    29041 actacatcct gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga
    29101 aacagcgcgg cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac
    29161 aacggatggc gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg
    29221 atagtatgtt catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata
    29281 tgcgaccatt gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagtccg
    29341 cgccgcaccg ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata
    29401 ttacacagcg gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag
    29461 taaatgccgg tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc
    29521 ggctggcgca taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac
    29581 tcatcgtcga gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac
    29641 agttgcagca gcatctggtt gagttcactg acacggcgtg taacgcggaa gtaatccttc
    29701 atcatccgct cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta
    29761 agttggcgat cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag
    29821 cgaatacgcc acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag
    29881 aagccaaacc cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat
    29941 tgcagagtgt gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg
    30001 tagctggtgc catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag
    30061 aacttgtcgg aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc
    30121 gcaacatcgc caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat
    30181 aacccttcca acatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca
    30241 tcccagagca gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta
    30301 cggcttaaaa tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca
    30361 ccgacggcaa ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt
    30421 tgcaggagct ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca
    30481 ttgtcaaagg catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag
    30541 acggttaatt catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtggag
    30601 agagcggtgt ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa
    30661 attatcgcat taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat
    30721 cgccgggatg gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac
    30781 aatagtatgc tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc
    30841 tttcatggtg cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat
    30901 ccccggtttc agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc
    30961 atggaagccg cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc
    31021 ttcgacaaat ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat
    31081 gcgtagcgcc aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt
    31141 cggcttaccg acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt
    31201 gacatcaatg ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg
    31261 gcacaccact tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca
    31321 ggcagaaacc gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc
    31381 ggtgctgacg cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg
    31441 gccagcgacg cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc
    31501 tgtccatcag cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac
    31561 ccgggcaaat tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa
    31621 tagaaaattc gacgtctgat gctgtacaca gcgccaacaa ttattggtgt ccacgacgta
    31681 tttgtggtat aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag
    31741 cgacaatctc actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt
    31801 ggggtcggta atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat
    31861 catggcaact gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac
    31921 ccgttactgg aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat
    31981 catcaacctt gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat
    32041 tgtttctcgc aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt
    32101 gaaagacgct gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat
    32161 gctgactaac tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca
    32221 gtctcaggac ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga
    32281 gctggagaaa ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc
    32341 tctgtttgta atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg
    32401 tattccggta tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat
    32461 cccgggtaac gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac
    32521 cgtacgtgaa ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc
    32581 tgagtaataa ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct
    32641 cgagaattcc cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca
    32701 tctgcggcgc gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt
    32761 agggggcctg catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca
    32821 gatcacatct ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg
    32881 cgtgagcgta ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc
    32941 gacatcgagc tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa
    33001 gcaggcaacg ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc
    33061 attctggaag ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc
    33121 gcagacaaag ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa
    33181 gcacagttcg aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt
    33241 cgccgcgttg ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc
    33301 ggcgttctgg ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac
    33361 gttgctgcaa gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa
    33421 aaagaatacc aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca
    33481 gagaaaatgg ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag
    33541 ccgttcgtta tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa
    33601 gtgactggct tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt
    33661 gcagcagaag ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg
    33721 gcggcttctt tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa
    33781 agcgacgtac aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa
    33841 acatggctac caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag
    33901 ctctgcaggg cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg
    33961 aaatcaaaga actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc
    34021 tgttccgtgg cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg
    34081 ggatgctggc gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg
    34141 tgaacgctcg tctgatgtcc gctattccat tgaatggcgt gtgcgatagc tacagctggg
    34201 cagaagctat cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta
    34261 acccgttctt taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg
    34321 tggtgctgaa agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa
    34381 ccgcaaccat gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca
    34441 tggacctggc ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata
    34501 tgaacaaacc gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca
    34561 cggaataatt cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg
    34621 taagtctggt tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa
    34681 ccggagcgta caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata
    34741 aaatagccta ataatccaga cgattacccg taatatgttt aatcagggct atacttagca
    34801 cacttccact gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg
    34861 tgattagcga tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca
    34921 aaacccaaat cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg
    34981 tcgtggaata ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag
    35041 attcccgtac tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag
    35101 cgattatggc ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc
    35161 cgctgccgcc gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag
    35221 cagaacaagc gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag
    35281 cactgttgaa agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac
    35341 agaaactgac tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag
    35401 aactgatgca gttctgattt cttgaacgac gaaaacgccg ctcagtagat ccttgcggat
    35461 cggctggcgg cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc
    35521 accattctgg gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat
    35581 cccgaacact tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa
    35641 cagtgcctgg aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt
    35701 cttaaaacga tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc
    35761 gcttgcgata tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct
    35821 gctgggctgt tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat
    35881 aaagaatcac tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg
    35941 caattgttac cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc
    36001 cagcataatc tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc
    36061 gggtctggtg gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat
    36121 caagcttgcc gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc
    36181 atgatgaaca aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc
    36241 cagatggaag tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac
    36301 ggcagtgttc tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg
    36361 gcatggccga atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg
    36421 ttgacatttg ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg
    36481 ttcgaacaag gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct
    36541 gcttttcttg cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg
    36601 gaaaaaatgg atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac
    36661 gcgagtgaag tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac
    36721 agagagtcgc gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg
    36781 taagtagttg gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata
    36841 ggcttcagta ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt
    36901 ctgctactca accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca
    36961 ttatggacgg caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata
    37021 aagccggggc aaaatccgtc cgccgggctg tctcttttgc ggccaacaac ggtattgagg
    37081 cgttaacgct gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg
    37141 cgttaatgga actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata
    37201 acgtgcgtct gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta
    37261 ttcgtaaatc tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga
    37321 actacggtgg acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc
    37381 aaggaaacct gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc
    37441 atgaactggc ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact
    37501 ttttgctttg gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt
    37561 tcgatgaaca agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg
    37621 gcggcaccga gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc
    37681 tgatatctgc ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg
    37741 tggggttcgc cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc
    37801 ttagcggttt taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt
    37861 tggcgctgat gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg
    37921 aaatctcact ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt
    37981 acccaggttc cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc
    38041 taaccattgt tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga
    38101 atcattacag tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact
    38161 ccggcgcata tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc
    38221 cgggtaaaac ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg
    38281 gttatggcat gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg
    38341 tcgcagcgtt agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag
    38401 gaattaagga cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata
    38461 gcctgacggc tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt
    38521 aacggaaggt aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg
    38581 tgtacttatc accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg
    38641 cgttgagcgt ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg
    38701 caccgaatat gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg
    38761 cgcagaaccg gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca
    38821 acgagcggcg attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta
    38881 ctggctggtt tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc
    38941 caattcgata gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg
    39001 tatcgaaacg cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga
    39061 aagcaccacc attacagtag cgccatttgg cagcgaccaa cggcgggatg taaagctcga
    39121 tttacgtcac tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg
    39181 tcctcgtggg ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag
    39241 caaggcaggt ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca
    39301 gtgggtgacc tttgtgatgc ttgtccggga taacccgggt aaatccttag cgttagaaat
    39361 cgaaaggcag gggagtccct tgtctttgac attaatcccg gggagtaaac cgggtaatgg
    39421 taaagcgatt ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa
    39481 agttgtacgc cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca
    39541 gctgatgaag ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa
    39601 caacctcagt gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt
    39661 agtttattac ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt
    39721 tccgttgccc gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg
    39781 cggaccggta tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt
    39841 gctgttaatg gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa
    39901 gaacgcataa taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc
    39961 gccaccgtat acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag
    40021 cgtgtcgccg ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat
    40081 gatgaagata tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt
    40141 cgcgtccttc gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc
    40201 agcattactt tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag
    40261 gcttctggtg tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa
    40321 ggtctggaag acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg
    40381 accccgctgc cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct
    40441 gaaatccagc aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct
    40501 catttccaac tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag
    40561 aaacagaaac tggcgggcga tcttgaaacc ctgcgcagct actatctgga ccgcggttat
    40621 gcccgtttca acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac
    40681 gtcacggtga acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc
    40741 aaccttgccg ggcactccgc tgaaattgag cagctgacta agatcgagcc gggcgagctg
    40801 tataacggca ctaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat
    40861 ggttatgcct atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt
    40921 aaattacgcg tgaacgttga tgtgggtaac cgtttctacg tgcgtaagat ccgttttgaa
    40981 ggtaacgata cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca
    41041 tggttgggga gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt
    41101 gaaactgtcg ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc
    41161 tacaaggtga aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa
    41221 agtggcgtga gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct
    41281 gttggtatca acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac
    41341 ccgtacttca ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag
    41401 gcagatgacg ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg
    41461 ggcttcccga ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc
    41521 ctgtccaaca tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat
    41581 ccgagcacct ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt
    41641 tggacctata acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg
    41701 accggtaaag tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg
    41761 gcgacttatg tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg
    41821 ggttatggtg atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt
    41881 ggttccagca ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc
    41941 ccgcatcagg ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc
    42001 gcgaaagacc tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc
    42061 ctcgagttca tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact
    42121 tcattcttct gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct
    42181 ggatatccgg actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa
    42241 tggatgtccc cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat
    42301 ggagacaagg cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca
    42361 aaggaatgta gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca
    42421 cgcaaagaac tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg
    42481 gttattagct gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat
    42541 tgcaatcgtc aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa
    42601 cacgctggaa aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct
    42661 gcaggctaaa atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga
    42721 aaaagacgtg atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga
    42781 tcgcgcacgt cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt
    42841 gaaatccgtt gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta
    42901 caacagcagc gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat
    42961 gccttcaatt cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg
    43021 cgatatcgtc atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt
    43081 catggttaac ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat
    43141 gacccaggac gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct
    43201 gacttacgcg cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc
    43261 acccagtgcg gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa
    43321 cgcggtgatt gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt
    43381 cgtaggtaaa aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta
    43441 ccatgagatc cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga
    43501 cggctttggt tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt
    43561 aattattggc gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga
    43621 tgacactgtt attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt
    43681 cgtgattggc gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat
    43741 tggtcgttac tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga
    43801 caaagtgacg gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta
    43861 ttcctcaggc attccgctgc aatccaacaa agtctggcgc aaaaccgctg cactggtgat
    43921 gaacattgat gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga
    43981 ctaacgttcc atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc
    44041 aggccgtgtt attattgtcg tttcttatat tttgacagga agagtatctt gactactaac
    44101 actcatactc tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta
    44161 ctggtggatc gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc
    44221 tctgtcaatg agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg
    44281 ctgattctgg aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa
    44341 ctggaaccgg gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg
    44401 gtcgtgcctg gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg
    44461 acccgtttta aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg
    44521 tgtgctcgta gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc
    44581 gccattgtgg aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc
    44641 gttggacccc atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat
    44701 ggtcatacta aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt
    44761 aaccaggatt tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc
    44821 attcgcgaaa gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg
    44881 ggcagcgaca acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac
    44941 cgctgtattc tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg
    45001 atcatcggcg gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt
    45061 ggcggctgct ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac
    45121 gcaacgccgt tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg
    45181 attaccgcta tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa
    45241 gtgaaaccgg aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat
    45301 ttctttgcac gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga
    45361 ttgccctggt cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc
    45421 tgaaagaacg tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg
    45481 aaggctgcga agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc
    45541 tcggtcgtct gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac
    45601 tgaagccaga tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta
    45661 acctcaaaaa gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc
    45721 gacagaaacg cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt
    45781 tcgaaaaagc gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg
    45841 ctgatgccat gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt
    45901 acgatgccca ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta
    45961 gtgccgattt cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg
    46021 tggtgccgct ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg
    46081 cgccagacct gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg
    46141 atgcggcact actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga
    46201 tggtggtggg atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa
    46261 ctgattatgt ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc
    46321 aggaagagtg tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga
    46381 aaaccagcca cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg
    46441 ccgatgagca ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc
    46501 gcacacgcag ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt
    46561 cgtcaccgct gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa
    46621 aaagctgagc gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag
    46681 ctggagtctg ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac
    46741 catgctggcg atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat
    46801 tgatggtaac cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag
    46861 ccgcgtacca gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat
    46921 ggcggcgctg gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac
    46981 cgcttttcat ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt
    47041 tgggcctgtc aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa
    47101 ccggaatctg aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc
    47161 gatgatcgat ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat
    47221 gccagcactg gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc
    47281 gggacatggc gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct
    47341 gctgggtgat gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa
    47401 tctggcgttg ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat
    47461 cgatcgcgac tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat
    47521 gggcgacgtc ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc
    47581 gttttatgaa gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc
    47641 ggatgaagaa agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt
    47701 cgtggcgact aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg
    47761 cgtcgcgatc cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc
    47821 gcagcaatat atgcgtagcg aagaggagat gtgtgagctg tttgccgaca tcccagaagc
    47881 ccttgccaac accgttgaga tcgccaaacg ctgtaacgta actatgcgtc ttggtgaata
    47941 cttcctgccg cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc
    48001 aaaagaaggc ctggaagagc gtctggcatt tttattccct gacgaggaag aacgtcttaa
    48061 acgccgcccg gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg
    48121 cttcccgggc tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt
    48181 accggtaggg ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat
    48241 caccgacctc gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg
    48301 tgtctccatg cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga
    48361 acacgtggcg gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat
    48421 ggcggcgaaa gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt
    48481 cgaccgtatc tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga
    48541 agccgagccg cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga
    48601 catggcgcgc aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt
    48661 tatcgcgccg accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca
    48721 tccggtcacc cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt
    48781 ccttggtttg cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg
    48841 ggcgaagaat ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag
    48901 cttcgacatg ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat
    48961 gaaggacctg atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc
    49021 actgttccgc cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca
    49081 tggtcgtgaa gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt
    49141 actggagcca acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt
    49201 gctttctggt tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa
    49261 gccggaagag atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat
    49321 caacgccgaa ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt
    49381 taacaaatcg cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc
    49441 gcactatccg gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa
    49501 ggtggtgggc ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat
    49561 aaactccggt ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg
    49621 cgccatcaaa ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg
    49681 cggctacttc cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg
    49741 gcgggtgctg gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc
    49801 ggcgctgatg aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga
    49861 agctatcggt caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca
    49921 atcctacgcc agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac
    49981 gttaggcctg tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta
    50041 tgtcggaggc gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc
    50101 tgcggggctc gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat
    50161 ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga
    50221 taaataccag caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt
    50281 tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc
    50341 ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca
    50401 gcttttaaac cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca
    50461 tctctactat cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt
    50521 ctctccgagc gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga
    50581 actggagttt gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc
    50641 gattgcagag ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa
    50701 actggatatt aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac
    50761 gcgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccgca
    50821 gcgtccttat accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg
    50881 cgaccgtgcg tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg
    50941 tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa
    51001 ctttggtatg ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga
    51061 acgctttaag atgcctatca tcacctttat cgacaccccg ggggcttatc ctggcgtggg
    51121 cgcagaagag cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct
    51181 tggcgtaccg gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat
    51241 tggcgtgggc gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga
    51301 aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat
    51361 gggtatcatc gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc
    51421 gctgggcggc gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct
    51481 ggcggatctg gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca
    51541 gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg
    51601 tggccctttt ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag
    51661 gaacacatga acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc
    51721 atcaaagaac tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac
    51781 agcgttgatt tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac
    51841 tgggatgagt acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg
    51901 ctttatgcct tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg
    51961 gcgctctggt tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt
    52021 cagtacacca acgaatatct tgataacatt acgccgccgt tcacgaaagc cttgtttacc
    52081 tacgtcaaag agcggaagta caccttttgt acgccggggc atatgggcgg caccgcatat
    52141 caaaaaagcc cggttggctg tctgttttat gattttttcg gcgggaatac ccttaaggct
    52201 gatgtctcta tttcggtcac cgagcttggt tcgttgcttg accacaccgg gccacacctg
    52261 gaagcggaag agtacatcgc gcggactttt ggcgcggaac agagttatat cgttaccaac
    52321 ggaacatcga cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg
    52381 ttgatcgacc gcaactgcca taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg
    52441 ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc tgggggggat cccgcgccgt
    52501 gaatttactc gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc acaatggccg
    52561 gttcatgcgg tgatcaccaa ctccacctat gatggtttgc tctacaacac cgactggatc
    52621 aaacagacgc tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtatactcat
    52681 tttcatccaa tctaccaggg taaaagtggt atgagcggcg agcgtgttgc gggaaaagtt
    52741 ttcttcgaaa cgcaatcgac ccacaaaatg ctggcggcgt catcgcaggc ttcactgatc
    52801 cacattaaag gcgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc
    52861 acctcgccca gttatcccat tgttgcttcg gttgagacgg cggcggcgat gctgcgtggt
    52921 aatccgggca aacggctgat taaccgttcg gtggagcggg cgctgcattt tcgcaaagag
    52981 gtccagcggc tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag
    53041 gtggatgaag ccgaatgcag gcccgttgcg cctggcgaac aatggcacgg ctttaacgat
    53101 gcggatgccg atcatatgtt tctcgatccg gttaaagtca ctattttgac accggggatg
    53161 gacgaacagg gcaatatgag tgaggagggg atcccggcgg cgctggtagc aaaattcctc
    53221 gacgaacgtg ggatcgtagt agagaaaacc ggcccttata acctgctgtt tctctttagt
    53281 attggcatcg ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaagcgc
    53341 tcttacgatc tcaacctgcg gatcaaaaat atgctgcccg atctctatgc tgaagatccc
    53401 gatttctacc gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt
    53461 aaacacgatc tttccggttt gatgttgcgg gcattcgata ctttgccgga aatgatcatg
    53521 acgccacatc aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa
    53581 caactggtcg gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg
    53641 ttgatgccag gagaaatgct gaccaaagag agccgcacgg tactcgattt tctactgatg
    53701 ctttgttccg tagggcaaca ttaccccggt tttgaaacgg atattcacgg cgcgaaacag
    53761 gacgaagacg gcgtttacca cgtacgagtc ctaaaaatgg cgggataact tgccagagcg
    53821 gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac
    53881 aggttcacca tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg
    53941 atattcttgg tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag
    54001 gggatttggc gcttaatggg caatatgtga ttgagctttt ctcttttccg ttcccgccgg
    54061 agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca
    54121 tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg
    54181 atccatacac gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac
    54241 tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta
    54301 ctgtaaaatc gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag
    54361 attctggtgg cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag
    54421 tggcggacgg aaaatccggg cgtcactctg cgcgctattc atgtgcatca cggtttaagt
    54481 gccaatgccg atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg
    54541 ctggtggtcg aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg
    54601 caggcacgtt atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg
    54661 caacatctcg acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct
    54721 gccgggcttt cggctatggc ggaagtttcg gagtttgctg gaacacggct tatccgcccg
    54781 ttgcttgccc gcacgcgggg ggaactggag cagtgggcat tggcgcatgg tttacgctgg
    54841 attgaagacg aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta
    54901 gtgccgttat tgcagcagcg ttggccgcat tttgcggaag caacggcccg cagcgccaca
    54961 ctttgtgctg aacaagagag tctgttggat gaactgctgg cggatgattt agcacactgc
    55021 caaacgtcgc aggggacgct gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc
    55081 gcggcgatta ttcgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg
    55141 ctggtgcgca tctggcagga agtggcgctg gcgcgggaag atgcttcccc ctgtttacgt
    55201 ttgggcgcct ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa atccgtcacc
    55261 gggcaaagcg aaaccattgt gctgtggcag acgtggctac aaccgctgga attaccggcg
    55321 gggctgggaa ccgtacagct tactgcggga ggcgatattc gccctccgcg tgcagacgaa
    55381 gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg caacggcgga
    55441 cgtaagctga agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg tgacaccacg
    55501 ccactgctgt tttatggaga aacgctgatt gcggcggcag gggtatttgt gacgcaagaa
    55561 ggtgtggctg aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct tagttaagtg
    55621 aaagccggat aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg attcgcttac
    55681 caccaccgta ccgatttcag ggtggctaaa gctggtaatt ttatccagac gcagctcgcg
    55741 ggtttcgcca gcggcctcga cgaccagata ctccacattt ttgcgggaga ctaaatcact
    55801 ggcttttgcc tgcaattttt cgccatcttt cagctcaagt gtcagcatta aatgatgctg
    55861 gcaggcgagc tcaagattat cgtaatcatc acaattaatt ggttgatacg tatcattcat
    55921 tgacataatc gctcaccagt aagtttgccg cagcgtatgc tgctttttcc ctgacagcct
    55981 cagaaagggc gtcgtcggca gccatttcat tcagcacttt caaaacgcag cccagcgcgt
    56041 ccggaacgta tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt attaactcac
    56101 aatatttttc cacatgccct cctgtcagca ctctgactta accgtggatg caagtctaag
    56161 cctacgaaga taaactctgt ttcgcaaggt gactatacca cactcatttc tgcaatatca
    56221 gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga gttttcttat
    56281 ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg gccgatctcg accgcaacca
    56341 gtttctcgat gcctctctga cgctggcgcg ccacccttca gaaacccagg agcgtatgat
    56401 gctgcgcttg ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt
    56461 gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat cacctgggca ttgatttgtg
    56521 gattgagctg gggctgccgg atgagcgacg aattaagaaa gcctgcaccc aggccgcaga
    56581 agtggcgctg tttacctata atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag
    56641 caaatgtgtg cagtttgcca atctttccgt ctggtatctg gacgatgaac aactggcgaa
    56701 agtaagcgcc tttgccgatc gtaccatgac gctgcaggca acgattcagg atggtgttat
    56761 ctggttatcg gatgataaga ataatctgga agttaactta accgcctggc aacaaccttc
    56821 atgattgtga tttcccgaca tgttgctatt cccgatggtg agcttgagat caccgccatt
    56881 cgtgcgcagg gcgctggcgg gcagcatgtt aataagacct caacggctat tcatctgcgt
    56941 tttgacattc gggcgtccag cctgccagag tattacaaag agcgtctgct cgccgccagc
    57001 catcatttga tcagcagtga tggcgtgatt gtcattaagg cacaggaata ccgcagtcag
    57061 gaactgaacc gcgaagccgc tctggcccgg ctggtggcag tgattaaaga tttaacaaaa
    57121 gaacaaaaag cccgacgacc cacgcggccc acccgtgcat cgaaagagcg caggctggca
    57181 tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag cggtcgggaa
    57241 taaaaagaag gaatggatgg tgaaaaaagc gatagtgaca gcgatggctg taatcagcct
    57301 ctttactctg atgggatgta ataatcgggc cgaagtcgat acgctttctc cggcgcaggc
    57361 tgccgaactg aaaccgatgc cgcaaagttg gcgcggcgtg ctaccgtgtg ccgattgcga
    57421 aggaatcgaa atctctctgt tcctcgaaaa agacggaaca tgggtgatga atgagcgtta
    57481 tctcggtgct cgtgaagaac cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc
    57541 tgacaagctg gtattaaccg acagcaaagg tgaaaagtca tattatcggg cgaaaggcga
    57601 tgcgctggag atgctcgatc gtgaaggcaa tccaattgaa tcgcagttca actatacgct
    57661 ggaaccggca caatccagtt tacctatgac gccgatgacc ctgcgaggca tgtattttta
    57721 tatggctgat gcggcgacct tcactgattg cgcgaccgga aaacgtttca tggtagcgaa
    57781 taacgcagag ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa aaccggtgtt
    57841 actgtcagta gaaggtcact ttacgcttga ggctaatccg gatactggtg cgccaaccaa
    57901 agtattagcg cccgatacgg cgggtaaatt ttacccgaat aaggattgca gtagtttggg
    57961 gcagtaaacc tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct gcgcttatca
    58021 ggcctacagc atataatgca acattttgaa ttagcacgat tttgtaggcc ggataaggcg
    58081 tttacgccgc atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca
    58141 aagcgacccg ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt
    58201 tctggcttaa acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc
    58261 cagcccgatt gcgccagtgt aagtggatgc ccggatttgg caaatgcatc agtgacaaat
    58321 tccgaacaaa accacgactt tttgtctcct tcgcacacac tgcttaactg cgctttcgcc
    58381 aggtcgctga cgcactgttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg
    58441 cgagtcacca taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg
    58501 attttattgg caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt
    58561 aagacgaaaa gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca
    58621 gcgcctgtcg cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag
    58681 gaagtgctga agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa
    58741 tcgccgggtt tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt
    58801 tgatgctgga atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg
    58861 tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaagcagg
    58921 cggcagtacg cctttggttt atccatttta tacaatccat gtaaaaaagg gccctgaaat
    58981 tcaggaccct ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc
    59041 agtcttaatt aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc
    59101 gaggttacgg tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa
    59161 catcacgccc ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag
    59221 ttcgctgtac agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg
    59281 cagaatcgcc acctggaacg gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta
    59341 gttctgctca atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt
    59401 caggatttgg ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt
    59461 ggtacccagc tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg
    59521 gccatccggg cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc
    59581 gacatcgcga tcccagttga taccgaagta gtgtttacca tcgatgttag caccagcagc
    59641 gaaatcactc atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg
    59701 acccagtgaa cccggaccgg ctttaaccac agcacgaatt tcttcttcgg tcgcgaaagt
    59761 cagcgggctt gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc
    59821 gcgcaccagc agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt
    59881 cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc
    59941 gttcggcgta tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc
    60001 gatagcttct gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc
    60061 gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc
    60121 ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc
    60181 cgcatacatt gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc
    60241 atctttcatc aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg
    60301 gaacttggtc tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt
    60361 acgaatcagg tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc
    60421 acggtcaaca aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca
    60481 caaatcggct ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc
    60541 ttcacgcacg atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata
    60601 taacccggag gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac
    60661 ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt
    60721 gttacggttc cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga
    60781 tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc
    60841 agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa atcatgtagc
    60901 cagacggcat aggttttgcc cgtttcctca cctttacgat aggccgggcg cgggtcctgc
    60961 gccagtactt cgcggataaa cagcgttaac tgcggataac gcttctccag cgtcaaaagc
    61021 tgcttttcga cctctgcggt aaaactcacc gccatctctg cagctggcgc actttgcgca
    61081 tagctggcac tggcatcggg aagcgattcg gcaaagggga gatacggttt gatatccact
    61141 accggcgtac catcgaccag atccagactg ccgagcttca gaatcacgct gcctttatgg
    61201 caaacaacct ctttcagctc taccagcgac atgccaattg ggttagggcg gaaagtagag
    61261 cgtgttgcga aaacccccat tctggcgtta ccgccgaggc gcggtggacg cacagtcgga
    61321 cgccagccgc cttccatcgt ttgatgaaag acgaaaagga tccataaatg gctgaatgct
    61381 tccaggccgc gaacggcgtc ggcctggttg taggaagcaa tgagatgcag ttcgccgttg
    61441 gcgcttttta ccagacctgg ctggcgcgga acggcgaatt tttctttata gggcgagcga
    61501 ataacgccta tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc
    61561 cgatacatac agcctgacga tagcagcccg gcgtaccgct ggtgacttcg cagctatgca
    61621 gtaatacagc attggctttc attttagagg cattgatttg catctgctta cgtgcggttg
    61681 gaatgctcgg cggagagtcc tgattagagg cctggcaaga gtcgccactg acttcaccga
    61741 gatcgcggaa cggtttgccg actaattctt ctgcattggt ataaattcgg accggcgtgg
    61801 cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg
    61861 gttcgacagg ggatctgctt aacatggaac agccgcttag catgagtgct actaaacaga
    61921 tcggtaaagc acgcatagta tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg
    61981 agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga atgatattac aaattgtgga
    62041 aacagcctaa aaattaccag cctttaacag cgccgccgtt aaacactttg tttgctgctt
    62101 cgtaaacttc atcagactga taagcctgga cgaatttctt cacgttctcg gcgtctttgt
    62161 tatcttcacg cgttacgatc aggtttacgt atggggagtc tttatcttcg acaaagatac
    62221 cgtctttcgc cggagtcagg ccaatctggc tggcataggt ggtattgata actgccagag
    62281 cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc ttccagttca acaattttca
    62341 gattttttgg gttctcaaca acatcaagaa cggtcggcag caggccaacg ccatctttca
    62401 gtttgatcaa gcccactttt tgcagcagca gcagtgaacg accaagatta gttggatcgt
    62461 ttggcacggc aacctgcgaa ccatcctgca gttcatccag tgatttgatt ttcttggagt
    62521 aaccagcaat cggataaaca aaagtgttgc ctactgcgat cagtttgtag ccacgatctt
    62581 tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc
    62641 tcaatgcttc gtttggcaga acatagtcgt tgaaggttac cagctcaacg tccaggccat
    62701 atttgtcttt cgcaactttc tgcgcgactt ctgctacctg ctgttcggca ccaacaatca
    62761 cgccgacttt aatgtggttt ggatcttttt catcctgacc gcagcctacc agtgccagtg
    62821 atccaatcag ggctcccact gccgcaaagg ttttgaattt gaacgccata ccttattcct
    62881 tcttcaatta tttatgttgt gttgaacgtt acttgcgagt gacagcccgg acgatgcggt
    62941 cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa taccagtacc gtattcatca
    63001 ccgtcgcgtt gtagccgatg tagccatact gatagccaat ctgacctaaa ccaccggcac
    63061 cgactgcacc acccatcgcg gaataaccga ccagggtaat cagggtgata gttgccgcat
    63121 tcaccagacc cggcaacgct tccggtaaca gcactttaca gacgatctgc attggcgtgg
    63181 cacccattgc gcgggaagct tcaattaacc cggttgggat ctccagcaga gcgttctcga
    63241 ccatacgggc aataaacggt gctgcaccaa cggttaacgg aacaattgct gcctgcaaac
    63301 caatcgatgt accgacaata acgcgggtaa acggaatcat ccataccagc aagataatga
    63361 acgggatgga acggaaaatg ttcacaatcg cagaaacggt acgatacagc ttcgcgttag
    63421 caataatttg ccccggacgc gtgacataaa gcagaacgcc aaccggcaga ccaatcacaa
    63481 acccaaaaaa tccggatacg aaggtcattg ccagcgtttc ccatacgcca cgaaccagca
    63541 gccacatcat cggctcagac ataacccagt acctctactt ttacatggtg ttcctgcagc
    63601 caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag catgatgccg
    63661 aatttaacgc caccggcgta gtccatctgc gcgctaataa tgttgttgtt gacgttgaaa
    63721 cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt gaccggtaaa ctccagacgc
    63781 agcatcggca cgcagtcagt aaatggctcc gtttgcagac gttcctggta atcttccggg
    63841 atatccagat gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt cggatgcgag
    63901 aacacttcac ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac cgccacgcaa
    63961 tcacaaatac gcttcacaac gtccatttcg tgggtgatca acagaatcgt caaacccaga
    64021 cggcggttaa tgtctttcag cagttcgaga atagaacgtg tcgttgccgg gtccagcgcg
    64081 ctggtggctt catcacacag caatactttg ggattgctgg ctaatgcacg ggcaatcgcc
    64141 acacgttgtt tctgcccacc ggaaagattc gacgggtagc tatcatgctt atcgccaaga
    64201 ccaaccaatg acagcaattc cgtcacgcga cgtttgatct cgtctttcgg tgtgttgtcc
    64261 agctccagcg gcagagccac gttgccaaaa acagtacgcg aagagagcag gttaaaatgc
    64321 tggaaaatca taccaatctg gcggcgagct ttggtcaact cggattctga cagcgtggtc
    64381 aattcctggc catcgaccag cacgctaccc tcggttgggc gctccagcag gtttacacaa
    64441 cgtataagcg tactcttacc cgcgcctgag gcaccgataa cgccataaat ttgtccagct
    64501 ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg gtggaacact
    64561 ttggtgatat tcgaaagttt tatcattgat tatttattat cgtcattaag ttagtcgtag
    64621 catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag gcatccagac
    64681 gtctaaatca atcaggttta tgcgaagagc actttcttgc aggtcgaaac atgcgatact
    64741 agcgtcccat gccttattaa ggagctataa aaggtggcga agagcgtacc cgcaattttt
    64801 cttgaccgtg atggcaccat taatgtcgat cacggctatg tccatgagat cgacaacttt
    64861 gaatttatcg acggtgttat tgacgccatg cgcgagctaa aaaaaatggg ctttgcgctg
    64921 gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc acagtttgaa
    64981 acgctgaccg agtggatgga ctggtcgctg gcggaccgag atgtcgatct ggatggtatc
    65041 tattattgcc cgcatcatcc gcaggggagt gttgaagagt ttcgccaggt ctgcgattgc
    65101 cgcaaaccac atccggggat gcttttgtca gcacgcgatt atttgcatat tgatatggcc
    65161 gcttcttata tggtgggcga taaattagaa gatatgcagg cagcggttgc ggcgaacgtg
    65221 ggaacaaaag tgctggtgcg tacgggtaaa cctattacac ctgaagcaga aaacgcggca
    65281 gattgggtgt taaatagcct ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg
    65341 gcgcaatgat taaaagatga acggttgaaa taaaaatgca tttttccgct tgtcttcctg
    65401 agccgactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    65461 agccggttcg gttgaagaga aaaaatcctg aaattcaggg ttgactctga aagaggaaag
    65521 tgtgatatac gccacctcgc gacagtgagc tgaaagctgc gtcgcaactg ctctttaaca
    65581 atttatcaga caatctgtgt gggcactcga agatacggat tcttaacgtc gcaagacga
//
LOCUS       DAJTRB010000008        65412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTRB010000008 DAJTRB010000000
VERSION     DAJTRB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.008"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            131..574
                     /locus_tag="OO330_000580"
     CDS             131..574
                     /locus_tag="OO330_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5881575.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="OO330_000581"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="OO330_000581"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5881576.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO330_000582"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO330_000582"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5881577.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO330_000583"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO330_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5881578.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO330_000584"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO330_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5881579.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO330_000585"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO330_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5881580.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="OO330_000586"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="OO330_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5881581.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="OO330_000587"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="OO330_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5881582.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTM
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="OO330_000588"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="OO330_000588"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5881583.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="OO330_000589"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="OO330_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5881584.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="OO330_000590"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="OO330_000590"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5881585.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="OO330_000591"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="OO330_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5881586.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="OO330_000592"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="OO330_000592"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5881587.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNENILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO330_000593"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO330_000593"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5881588.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="OO330_000594"
     CDS             13934..14314
                     /locus_tag="OO330_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5881589.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="OO330_000595"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="OO330_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5881590.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO330_000596"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO330_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5881591.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO330_000597"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO330_000597"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5881592.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAVVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="OO330_000598"
     tRNA            complement(17415..17490)
                     /locus_tag="OO330_000598"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="OO330_000599"
     tRNA            complement(17494..17569)
                     /locus_tag="OO330_000599"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="OO330_000600"
     tRNA            complement(17621..17696)
                     /locus_tag="OO330_000600"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="OO330_000601"
     tRNA            complement(17699..17774)
                     /locus_tag="OO330_000601"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17911..17986)
                     /locus_tag="OO330_000602"
     tRNA            complement(17911..17986)
                     /locus_tag="OO330_000602"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17951..17953),aa:Lys,seq:ttt)
     gene            complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="OO330_000603"
     CDS             complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="OO330_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5881593.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18952..19473)
                     /gene="pal"
                     /locus_tag="OO330_000604"
     CDS             complement(18952..19473)
                     /gene="pal"
                     /locus_tag="OO330_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5881594.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMFSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="OO330_000605"
     CDS             complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="OO330_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5881595.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20933..22141)
                     /gene="tolA"
                     /locus_tag="OO330_000606"
     CDS             complement(20933..22141)
                     /gene="tolA"
                     /locus_tag="OO330_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5881596.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEAD
                     DIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFY
                     DASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEV
                     FKNAPLDFKP"
     gene            complement(22206..22634)
                     /gene="tolR"
                     /locus_tag="OO330_000607"
     CDS             complement(22206..22634)
                     /gene="tolR"
                     /locus_tag="OO330_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5881597.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22638..23330)
                     /gene="tolQ"
                     /locus_tag="OO330_000608"
     CDS             complement(22638..23330)
                     /gene="tolQ"
                     /locus_tag="OO330_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5881598.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23327..23731)
                     /gene="ybgC"
                     /locus_tag="OO330_000609"
     CDS             complement(23327..23731)
                     /gene="ybgC"
                     /locus_tag="OO330_000609"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5881599.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23881..24174)
                     /gene="ybgE"
                     /locus_tag="OO330_000610"
     CDS             complement(23881..24174)
                     /gene="ybgE"
                     /locus_tag="OO330_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5881600.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24174..24287)
                     /gene="cydX"
                     /locus_tag="OO330_000611"
     CDS             complement(24174..24287)
                     /gene="cydX"
                     /locus_tag="OO330_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5881601.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24302..25441)
                     /gene="cydB"
                     /locus_tag="OO330_000612"
     CDS             complement(24302..25441)
                     /gene="cydB"
                     /locus_tag="OO330_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5881602.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25457..27025)
                     /gene="cydA"
                     /locus_tag="OO330_000613"
     CDS             complement(25457..27025)
                     /gene="cydA"
                     /locus_tag="OO330_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5881603.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27874..30507)
                     /gene="mngB"
                     /locus_tag="OO330_000614"
     CDS             complement(27874..30507)
                     /gene="mngB"
                     /locus_tag="OO330_000614"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5881604.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKILWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30525..32441)
                     /gene="mngA"
                     /locus_tag="OO330_000615"
     CDS             complement(30525..32441)
                     /gene="mngA"
                     /locus_tag="OO330_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5881605.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32611..33333
                     /gene="mngR"
                     /locus_tag="OO330_000616"
     CDS             32611..33333
                     /gene="mngR"
                     /locus_tag="OO330_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5881606.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33436..34305)
                     /gene="sucD"
                     /locus_tag="OO330_000617"
     CDS             complement(33436..34305)
                     /gene="sucD"
                     /locus_tag="OO330_000617"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5881607.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34305..35471)
                     /gene="sucC"
                     /locus_tag="OO330_000618"
     CDS             complement(34305..35471)
                     /gene="sucC"
                     /locus_tag="OO330_000618"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5881608.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIITAKGLTDAAQQVVAAVEGK"
     gene            complement(35565..36782)
                     /gene="odhB"
                     /locus_tag="OO330_000619"
     CDS             complement(35565..36782)
                     /gene="odhB"
                     /locus_tag="OO330_000619"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5881609.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36797..39598)
                     /gene="sucA"
                     /locus_tag="OO330_000620"
     CDS             complement(36797..39598)
                     /gene="sucA"
                     /locus_tag="OO330_000620"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5881610.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39899..40615)
                     /gene="sdhB"
                     /locus_tag="OO330_000621"
     CDS             complement(39899..40615)
                     /gene="sdhB"
                     /locus_tag="OO330_000621"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5881611.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40631..42397)
                     /gene="sdhA"
                     /locus_tag="OO330_000622"
     CDS             complement(40631..42397)
                     /gene="sdhA"
                     /locus_tag="OO330_000622"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5881612.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42397..42744)
                     /gene="sdhD"
                     /locus_tag="OO330_000623"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42397..42744)
                     /gene="sdhD"
                     /locus_tag="OO330_000623"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5881613.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42738..43142)
                     /gene="sdhC"
                     /locus_tag="OO330_000624"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42738..43142)
                     /gene="sdhC"
                     /locus_tag="OO330_000624"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5881614.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43523..43630
                     /locus_tag="OO330_000625"
     CDS             43523..43630
                     /locus_tag="OO330_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881615.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43836..45119
                     /gene="gltA"
                     /locus_tag="OO330_000626"
     CDS             43836..45119
                     /gene="gltA"
                     /locus_tag="OO330_000626"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5881616.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45510..46076
                     /locus_tag="OO330_000627"
     CDS             45510..46076
                     /locus_tag="OO330_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5881617.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46127..48586
                     /locus_tag="OO330_000628"
     CDS             46127..48586
                     /locus_tag="OO330_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5881618.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48601..49332
                     /locus_tag="OO330_000629"
     CDS             48601..49332
                     /locus_tag="OO330_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5881619.1"
                     /translation="MTFMKGLPLLLLVANLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49329..50376
                     /locus_tag="OO330_000630"
                     /pseudo
     CDS             49329..50376
                     /locus_tag="OO330_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50528..51574
                     /locus_tag="OO330_000631"
     CDS             50528..51574
                     /locus_tag="OO330_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5881620.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51571..52362)
                     /gene="nei"
                     /locus_tag="OO330_000632"
     CDS             complement(51571..52362)
                     /gene="nei"
                     /locus_tag="OO330_000632"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5881621.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52398..53132)
                     /gene="pxpA"
                     /locus_tag="OO330_000633"
     CDS             complement(52398..53132)
                     /gene="pxpA"
                     /locus_tag="OO330_000633"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5881622.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53122..54054)
                     /gene="pxpC"
                     /locus_tag="OO330_000634"
                     /pseudo
     CDS             complement(53122..54054)
                     /gene="pxpC"
                     /locus_tag="OO330_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54048..54704)
                     /gene="pxpB"
                     /locus_tag="OO330_000635"
     CDS             complement(54048..54704)
                     /gene="pxpB"
                     /locus_tag="OO330_000635"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5881623.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54727..55470)
                     /gene="ybgI"
                     /locus_tag="OO330_000636"
     CDS             complement(54727..55470)
                     /gene="ybgI"
                     /locus_tag="OO330_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5881624.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55741..57222
                     /gene="dtpD"
                     /locus_tag="OO330_000637"
     CDS             55741..57222
                     /gene="dtpD"
                     /locus_tag="OO330_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5881625.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57372..58790)
                     /gene="phrB"
                     /locus_tag="OO330_000638"
     CDS             complement(57372..58790)
                     /gene="phrB"
                     /locus_tag="OO330_000638"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5881626.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58787..59296)
                     /locus_tag="OO330_000639"
     CDS             complement(58787..59296)
                     /locus_tag="OO330_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5881627.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59425..59712
                     /locus_tag="OO330_000640"
     CDS             59425..59712
                     /locus_tag="OO330_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5881628.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59767..60147)
                     /locus_tag="OO330_000641"
     CDS             complement(59767..60147)
                     /locus_tag="OO330_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881629.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60150..64814)
                     /locus_tag="OO330_000642"
     CDS             complement(60150..64814)
                     /locus_tag="OO330_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5881630.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETVFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPFNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYRFRHDVLDRLAEQINPDGCRQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDKLGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGRITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPW
                     ET"
     gene            complement(64833..65036)
                     /locus_tag="OO330_000643"
                     /pseudo
     CDS             complement(64833..65036)
                     /locus_tag="OO330_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15865 a        17720 c        15968 g        15859 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc aggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg ccaagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgctaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactatggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccacaagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatctcg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaaac attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccacagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt acattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt ggtgggtcgt
    17821 gcaggatgac tcgacttcgt ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc
    17881 acgttttact accgcttcca ccttagcgat tggtgggtcg tgcaggattc gaacctgcga
    17941 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct
    18001 ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc
    18061 gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa
    18121 ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg
    18181 tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt
    18241 tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt
    18301 tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt
    18361 acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc
    18421 agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc
    18481 ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc
    18541 gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgtcg caccgctttg
    18601 atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag
    18661 gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc
    18721 acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag
    18781 ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga
    18841 gccgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag
    18901 taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac
    18961 cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt
    19021 ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta
    19081 catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg
    19141 ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc
    19201 atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta
    19261 aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaaaacat
    19321 gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc
    19381 gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat
    19441 catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta
    19501 attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa
    19561 gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag
    19621 aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg
    19681 tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc
    19741 caccattgga gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcttggt
    19801 tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa
    19861 cctgcggacg accggcctgg tcagaagtaa atgccaggtt ctggctatcc gggaaccagg
    19921 tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat
    19981 ccattacgta caggttcaga ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt
    20041 ctggcgagaa tgcaggtgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc
    20101 catttgccag cgtctgaata accagcgcgg aacgaccgct ttcgaaggtc acataagcca
    20161 gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgcggtgaa cggtgaacga
    20221 caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt
    20281 tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt
    20341 caaacacttc atcactggcg gtatgaccag cataacgcag ccactgcttg ttcactttgt
    20401 acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa
    20461 cattgtaaga accatccgga ttcggagtga cctgaccgac aactacagcg tcaatgccca
    20521 gtgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag
    20581 cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa
    20641 tatcttcagg tgccgcacca ggccccgccc actggaaagg aacaacacca ataggacgac
    20701 cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca
    20761 gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac
    20821 cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa
    20881 ccccgtggca accggtgcct gatgttgacc gtccgaacag tcaacatcgc gattacggtt
    20941 tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga
    21001 tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag
    21061 gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc
    21121 ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat
    21181 agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc ccggcaggcg
    21241 aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct
    21301 caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg
    21361 cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag
    21421 ctgctgcctt atcagcagcc gctttttctg ctgctttttt gtcggctgca gccttttcag
    21481 cagccgcttt cttctcagct tctgccttgg ctttttcagc agcttcagtt gccgctttct
    21541 ttcttgcttc agctgcagct gcttctgccg cttccgcttt cttcttcagt gccgcagcgg
    21601 ctacctcggc ttttttctgc gcttcggctg cggctttggc ggcttctgct tctgcttttt
    21661 tcttcgcgtc tgcagccgct ttcttcgctg cttcttccgc agctttagca tctgccgccg
    21721 ctttcgctgc cgccacttca gcttgcttct gctttaactc ggcctgtttt gcggcttctt
    21781 cagcctgctt tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt
    21841 cctgttcagc cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc ttcatcttgc
    21901 gctgttcatc agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa
    21961 ctaccgcacc tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg
    22021 aagcttctat attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca
    22081 gcactgctga aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca
    22141 ctctcggttt ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca
    22201 gatgtttaga taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag
    22261 ttcagtgctt taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc
    22321 gttttcgggt tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa
    22381 cgctccaggc gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca
    22441 atcactggcg gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg
    22501 acctccacgc tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc
    22561 agtacgtcca gcaacggaac aatgttgatt tcggacttga gatcgcgacg acctcgtcca
    22621 cgcgctctgg ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc
    22681 agaatcgcgg taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc
    22741 tggttgagac ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgccgtc
    22801 gcaatcaacg cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc
    22861 ccgagggcga taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg
    22921 ctgatggagc caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg
    22981 ttcatggaga tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta
    23041 ttggcacgat gcaggcgcac aaactctttg aacccgctgt aaaagatttg ttccgaacca
    23101 gtcagattat cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac
    23161 cagaatttat cttcaaacgc ttcggcttca cgcgccgctg cgttaagaat acgggttcgc
    23221 tggataataa tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta
    23281 accagaaggc tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact
    23341 ccgcgacaat agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa
    23401 ccagaacctc tgcttcattc agcaaagtat tctcggcgtt gacaatacgt tgcgtgaaaa
    23461 ccaaagaggt gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc
    23521 gcgcaggtgc gtaatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca
    23581 tcagcgcctg ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa
    23641 aagcgacgta actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc
    23701 gaaccggcca tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta
    23761 gagcttttaa acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta
    23821 agggcgcgta aaaatttatg ggcctggagc ggcccataat tttttaaaga gaattctgat
    23881 ttaaaagaag aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa
    23941 aatcccttgc caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca
    24001 gacggcccac atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa
    24061 acgagatggg tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc
    24121 ccgcaggggg cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg
    24181 tcttcttgac cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca
    24241 aacgaacagg caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta
    24301 cttagtacag agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt
    24361 tccagtaaca ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg
    24421 tcatgacgtt aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca
    24481 tcatggtgct ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc
    24541 aggccagcgt cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca
    24601 ggatggtcag cagcagcaga accacaccca gtgccggaat agcccacaga attggcgtgt
    24661 tgttgaagtt aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag
    24721 aggctgcgta atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc
    24781 atacgccagc cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac
    24841 gggtacgcag gtgcagttcg cccacggtac gcatttgcag ataggttgcg ccctgagtaa
    24901 tgatcatccc tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga
    24961 agttaccggt gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca
    25021 ggttaccgaa cgctacacca attaccagcg gcggaacgaa gctaccaatg aagatgcccc
    25081 agtcccacat gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg
    25141 gacggaagaa caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag
    25201 cggcatagac catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct
    25261 ggttaccgtc ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac
    25321 gaccgaggaa acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac
    25381 caatcagcag aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca
    25441 tttgacgact cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat
    25501 agcgaccggt tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt
    25561 ctgccaccag gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc
    25621 ctgcggtcag tgacgaattc gccacagctg tcggcagcac ttcaccgata gcccacggtt
    25681 ggcggccata ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac
    25741 cgtacagcgc ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc
    25801 agaaagagag cgcgatgatt gccagcagca ggaagccaca cgccaccatg atacggaacg
    25861 caaagtacag cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt
    25921 cagtcgcatc agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt
    25981 tcttcatgct attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt
    26041 cgagcagaga gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct
    26101 ctttcaggcc gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag
    26161 ggatctgaat cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacaaagtaa
    26221 aggcagcagg cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct
    26281 gcacgtcgcc catttcgtag ccggattcat cacccagaac aataacagac agaacagcag
    26341 ccataccgaa gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt
    26401 tcagcatata ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg
    26461 ctacagtgtg aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca
    26521 ccatctccat acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc
    26581 cgttcgcaac cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga
    26641 cacacatatg ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg
    26701 tggattcgag gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc
    26761 ctacatagtg ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg
    26821 tagccacacc cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt
    26881 tataaatctg tttgccggag aggacgtaga ccgtttccat aatggccagg aggaacgcca
    26941 taccgagcgt cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc
    27001 gcgacagttc gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga
    27061 gtgaaccccg ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc
    27121 atcataccat gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa
    27181 aaactcaaat tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt
    27241 tgttgaagca aatttgcatt tttcgacggt gatcaattta tagctaaatt gccgcctttc
    27301 agccaatttg atcgagaaca atttatctct ttttgatgcc catttccaag acttatacat
    27361 tgataaatat caatttctac aaattatgtt aatcatgttt caacaaggta actttagtga
    27421 tatttatgtt taaaatgtgt gaattataag actgaaatta gtttcggttg gcaattaaag
    27481 ataaaaagag acgaagcaaa aacagcacgg ttgaccttaa ttcttttttt ggtcactggc
    27541 taatacgacg cagagtttca ggattattaa taaaggcata aatctccccc ccgccgatat
    27601 taacatttaa ttaacttata attatattat aattaactca cccgtggtta ttattttgtt
    27661 aaaaataaag ccacatatac gtagcttgag agtgaagata aacacaaaat tacctgatgc
    27721 gctacgcttg ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag
    27781 accgaataaa gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg
    27841 gccgcgcata tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg
    27901 tgactggccg ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat
    27961 gtgttcatcc atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc
    28021 atcacaggtt gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc
    28081 agctttctta agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact
    28141 ttccggcacg ttgaatccgg ctttgttgag cttcatcgca tcccatagga ttttgttgta
    28201 acactgtact ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt
    28261 accggtataa ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg
    28321 gactggcatt ttaattcccg aaggccgcct aggccttaaa agcagatctt ctttccccag
    28381 taagcccacg ccgcgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc
    28441 aaattcacgt agcccttcgc taaagacagc catgccgtta cgcccttcct gtaaggctgc
    28501 atagttgagc atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt
    28561 gttcattgca ctgtcgttca cggggcgcgt tagcgaacca aactgggtat ctgccagaac
    28621 actatcggtg ttaaaagatg tagggatcag gacgcgaagg cgatgatcgt cagcctggtt
    28681 atcaaggttg atatccacat caatgcgcct gttgttatga ctaagagtga caaccaactc
    28741 gacatctact ctgccagtgg attgtcttgc gctgcgttgt tgcagattac gcggcaccgc
    28801 catttcatag cggataacag ccctgctctg ccaggcctca tgaataatct cgcattgcgg
    28861 tttcgcattc gccgaagtaa ttacccactc ttcttttgcg ggtgaatagt catattcatc
    28921 accatcatca gagctttctt caatttgcaa cacccggtca tagcgcacac cgctatcttt
    28981 atctaccagt tgtagcgttc catcctcatt gagcgcaatt tgccagaaag cattttccag
    29041 tatcccttca gcgtcacttt ttgccgcaat cacgttgcca ggctgattcg cttcgatata
    29101 aagcgtgcga tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa
    29161 gggatcgtaa ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg
    29221 ggcatggcga ataaaatacg gtacaggctg accgcgatcg tctcgtagat taaactggct
    29281 ggcgcgcagc cgcacagtgg tgttgatcac ttcttcccgc ggccagggca tcaggttaaa
    29341 caggacgagt ttgtcggcgt cgctctgcgg catgttgtcg gcgattttac gcatatagaa
    29401 acgcagcaga ttatccgcca tgtcttcagc cagttcgaag cgggcgacga tttcgcgatg
    29461 aactttgtca ctacagcagc agccgatgct gtcgtgggca tgatttttta agatctcttt
    29521 ccacattttc tccagcaagc cgtggtggta tccaaaaccc aacgtcctgg ccagcgttgc
    29581 cagcggttcc agcagattaa caatcttatt ttcaatacgt gcgtgggcaa ctttgatatc
    29641 catgcgcgtg gaaccgatgg tgcgatgcac gcgcatatat ttgccatcaa taaattcccc
    29701 tttcagggtt gccagattat ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg
    29761 gctcatcaca aactttcgtt gaggataaat ttcgcgcagt ttctccatca cttcgaagat
    29821 attttgctgc aatggcatct gatcatgccc attcggcagc aaaatctctt tggttacaga
    29881 cgctttttcc agcacgtcaa aataactgtc gaggcgtttg cgtaatccgt tttcgtccgc
    29941 aggtaagtac ttaccgatgg cataacccaa cggcatcacc tgcgccgtca cttcgctacc
    30001 gtcactgctt tgccacaaaa actcggtttt atccgtaccg tggcgctcag aacatccacg
    30061 ccagaacatg gtgcaggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat
    30121 gccaaaggaa tcgggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg
    30181 cattccgtac atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg
    30241 ggtataccag gggccgataa tcaacttgcc ggcttctacc agtttcttca cgcggtcttt
    30301 gttttccggt ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta
    30361 atatttgtat tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag
    30421 cagaatgcgt gactcttcgg tggtgaaata ccactcccga tcccagtgca tatgcggggt
    30481 gatgtgaacg cgagataccg ctttcatcgt cgtttccttt ttgtttatgg cattacgcca
    30541 tcagtcagat agttgccatg cttaaccgcg tgacgccgcc agatcaggag aattgcagtc
    30601 gagattgcag cccccaccaa tgccgcacca aaccagccaa ttgccgccat aacaccgccc
    30661 gcgccattat catgaagtaa aaagagcgag aaaatgccag caccgggtgt cgaaaggccg
    30721 atattcatcg caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata
    30781 acccgcagcg gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct
    30841 aacagccagg tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc
    30901 atcgtggaag cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac
    30961 gggccgtaaa cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttattcact
    31021 ggccctccaa ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta
    31081 cttcctgaca gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttcc
    31141 cccaccacaa acagcatcag actgcccgct cccaatgtac cgagcaccgg gtagagataa
    31201 aaagtcagga agccattgaa tttactgcta agacgcaagt gatttttcat ccagcgtatc
    31261 aagtaaccgg ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg
    31321 gcggcaagtc cagccgcaaa gcctggcgct aacgccggtt tatctgccag cgaataggcg
    31381 gtataggctg ccagcaccgg taccatcaaa attccgagca gcccgccgcc cagtttgcgg
    31441 tacatccaca gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac
    31501 agtaataccg cgaccgccag caccgtcccc cccgcgacaa tcaacggtac ggcaaaagag
    31561 atcccgctca acagtgcctg tttcagctcc gttttgacgc ttttcaccgg ttgcgtatct
    31621 tgctgtaagg tacgcgtctc atcgccacgc tcgagggtaa gcgattgttg gatcaacgct
    31681 tctgcatggc gaatcggctc ggcaacaggc acagaaagcg caggaatgcc gttaaaacgc
    31741 tcactctcct tgatggcgac ttcagccgca aaaatacagg cagttgcact attgagttga
    31801 tccgccgtta aacgcccttc aataccgtta gcaccttgct tttcaacgta gacattcacg
    31861 ccgagtttgc gtccggcttt ttccagatac tccgcagcca tgtaggtgtg agcaataccc
    31921 gccgggcagg ccgttacgca aacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc
    31981 gtgcctccct tgtcatccag cgccgagagc aattcatcag gcgtcgtcgc cgactgtata
    32041 cgcgcccgaa tctcatcatc cgccaggcgc gtggtcagcg ctgtcagcag ttgcatgtgc
    32101 gtagtacccg cttcattgag gggaatcgcc agcaacacca ctaaatcaac cgcttccggg
    32161 ccatccacgc cttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct
    32221 tttaccgcag cagttttgcc atgcggcaca gccaaccctt cacttaaggc ggtcgggcca
    32281 aggctttcac ggcgatacac ttctttcaga aattgctcag tactggaaat tttccccaga
    32341 gcagcaagac gttgagtcaa cgcgtgaatg gcctcttcac ggctggtaaa gcgcgcattc
    32401 agacacaacg catcgcggtg ggtcagagtc gtcaggttca tcatgatcct cacttgatcg
    32461 tttttatagt cgcgccagtg tgcccgacaa aacaatacca ttgcgtaatc aatttcactt
    32521 gattgaaatt atttgcattt tatttgtatt gtttacagga tgaatttatc gggcataatg
    32581 gctaaattcg tattaatgag atgctaaata atgggacaca aacccttata ccgacagatt
    32641 gctgatcgca ttcgtgagca aattgcgcgt ggcgagctta aacctggcga tgcgttacct
    32701 accgaatcgg cattacaaac agagtttggc gtcagccggg ttacggtgcg tcaggctttg
    32761 cgccagttag tcgagcaaca gatcctcgaa agcattcagg gcagcgggac ttacgtcaaa
    32821 gaagagcggg tcaattacga tatttttcag ttaaccagtt ttgatgaaaa actgtcggac
    32881 cgtcacgtcg atacacacag tgaagttctg atatttgaag tgattccggc tgacgatttt
    32941 cttcagcaac agctacaaat caccgtgcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc
    33001 tatcgcaagc aaaagccaat ggcgctggaa gaaacctgga tgccgcttgc gttgttcccg
    33061 gatctcacct ggcaggtcat ggaaaattcg aaatatcact ttattgaaga agtgaagaag
    33121 atggttatcg atcgtagcga acaggaaatc attcctctga tgccaaccga agagatgagc
    33181 cgtctgctta atatcagcca gacaaagccg attcttgaaa aagtatcgcg tggatattta
    33241 gttgatggtc gggtgtttga atatagccgc aacgctttta ataccgatga ctataaattt
    33301 accctgatag cgcagcgaaa atcatcgcga taagcacaaa aaggccatca tacgatggcc
    33361 aaccatgtcg aaaccgacag cccttcgcca ttcctgtaga gggaagcggc gagggctatt
    33421 tcttattaca gatatttatt tcagaacagt tttcagtgct tcaccgatat ccgccaagct
    33481 gcgaacggtt ttcacgcctg cggcttccag agcagcgaat ttctcatccg cagtcccttt
    33541 cccaccggca atgatcgcac ccgcgtggcc catacgtttg cctttcggcg cagtcacacc
    33601 agcgatgtaa cccacaactg gcttggtaac atgctctttg atgtacgctg ccgcttcttc
    33661 ttcagcgcta ccgccgatct caccgatcat cacgatcgct tcggtctgcg gatctttttc
    33721 gaacatttcg agaatgtcga taaagttaga gccagggatc gggtcaccgc cgataccgac
    33781 acaggtcgac tgaccgaaac cgtaatccgt ggtctgttta accgcttcat aggtcagtgt
    33841 accggaacgg gaaacgatac ccactttacc cggtttgtga atgtgacccg gctggatacc
    33901 aattttgcat tcacccgggg tgataacgcc tgggcagttc gggccgatca tacgaacgcc
    33961 tgcttcgtcc agtttcactt tcacggtcag catatccagc gtcgggatgc cttcagtgat
    34021 ggtgataatc agtttgatgc ctgcatcgat agcttccaga atggagtctt tgcagaacgg
    34081 tgctggtacg taaataacag acgcggaagc gccagtagca gcaacagctt cacgcacggt
    34141 gttgaacacc ggcaggccga ggtgggtagt gccgccttta cctggagtta cgccaccaac
    34201 cattttagtg ccgtatgcaa tggcctgttc tgagtggaaa gtcccctggc taccggtaaa
    34261 gccctggcag ataaccttgg tgtttttatc aattaaaatg gacattattt cccctccact
    34321 gcggcaacaa cctgctgagc tgcatccgtc agaccttttg ctgtaataat attcaggccg
    34381 ctgtcagcca gtttcttcgc gccgagttcg gcgttgttac cttccagacg taccacgacc
    34441 ggtacgttaa cacccacttc cgctaccgcg ccgatgatac cgtcagcgat caggtcgcaa
    34501 cgaacgatac cgccgaagat gttaaccaga acggctttca ctttgtcgtc agagaggatg
    34561 attttgaacg cttcggttac acgttctttg gttgcgccgc cgccaacgtc caggaagtta
    34621 gccggttcgc cgccgtgcag tttaactatg tccatcgtac ccatcgccag acctgcgccg
    34681 ttaaccatac aaccgatgtt accgtccagc gcaacgtagt tcagttccca ctgtgcagcc
    34741 tgtgcttcac gcggatcttc ctgcgactgg tcacgcattt cgcgcagatc aggctggcgg
    34801 aacagggcgt tgccatcagc gcccagtttg ccgtcgaggc aaatcagatc gccctgtttg
    34861 gtgatgacca gcgggttgat ttcgatcagc gccaggtcgc gctccaggaa aatggtcgcc
    34921 aggcccatga agattttggt gaactgctga accagtttac cttccagacc cagtttgaac
    34981 gccagctcgc gtccctgata cggcatcggg ccagtcagcg gatcaagcgc aactttatgg
    35041 atcaggtgcg gagtttcttc cgccactttt tcgatttcca cgccgccttc ggtggaggcc
    35101 ataaagacca cgcgacggga actacggtct acaacggcac cgagatacag ctctttagcg
    35161 atatcggtcg ccgcttcaac cagaatctgg ttaaccggtt ggccattggc atctgtttga
    35221 tacgttacca gacgcttgcc cagccagttt tctgcaaaag cacggatgtc ttctttgctg
    35281 tttacaactt tcacaccgcc cgctttaccg cggccaccag cgtgaacctg acatttcact
    35341 acccacggac cggcaccgat ttttgaagcg gcttcttctg cttcgcgcgg agtagtacag
    35401 gcataaccca ccggtgctgg taagccatag cgggcaaaca gttgttttgc ctgatattca
    35461 tgtaagttca tgtgttctgt ccatccttca gtaatcgtta tcttttaaac cgtagaccga
    35521 caccctatcc acgtcggtct acagtgcagg tgaagcttaa actactacac gtccagcagc
    35581 agacgcgtcg gctcttccag taactctttg atcgttacca gaaagcccac ggattcgcga
    35641 ccatcgatca gacggtgatc gtaggacagc gccaggtaca tcatcggcag gatctcaacc
    35701 tgaccattca ccgccatcgg acgatctttg atagcgtgca tacccagaat tgcgctctgc
    35761 ggcgggttga tgatcggcgt agacatcagg gaaccgaaca caccaccgtt ggtgatggtg
    35821 aagttaccac cggtcagatc ttcaaccgtc agcttgccat cacggccttt gactgccagc
    35881 tctttgattt tcttctcgat gtctgccatg ccgagggtat cgacatcacg cagaaccggc
    35941 gtcaccaggc cgcgcggcgt agaaaccgcc atgctgacgt cgaaatagtt gtggtaaacc
    36001 acgtcatcgc cgtcgataga cgcgttcact tccgggtaac gtttcagggc ttcaaccacc
    36061 gctttcacgt agaaggacat aaagcccaga cggatgccgt ggcgtttttc aaacgcttca
    36121 ccgtactgct tacgcagatc cataatcggc ttcatgttga cttcgttgaa cgtggtcagc
    36181 atggcggtgg agtttttcgc ttccagcaga cgctctgcca cacgcttacg caggcgagtc
    36241 atcgggacac gtttttcact acgtgcagcc agagccggtt gcgccgccgg agcagccgct
    36301 gccggagcag actctttcgc cggggctttc gccagatgtt tttccacatc ttcacgagtc
    36361 agacgaccac ccacaccggt gcctttaatg gcgctggcgt cgagattgtg ttcagccagc
    36421 aggcgacgga tcgccgggct taacgcatcg ttgttttgct cttccagaga cgcctgctgg
    36481 cgttgcgccg gagtggacgc tttctcttca gatttggcgc tggtttcttt accggcgctg
    36541 ttgccttcac gcaggcgacc aaggatctga cgagacgtta ccgttgtacc ttcatcttcc
    36601 agaaccgcat ccagaatgcc gtctgctgat gccggtactt ccagtaccac tttgtcagtt
    36661 tcgatttcta ccagcacttc atcacgtacg actgcgtcgc cgggtttttt atgccaggtt
    36721 gcgacggtgg catcggctac ggattcaggc aggtcaggga ccagaatatc tacgctactc
    36781 attgtgtatc ctttatttat tcgacgttca gcgcgtcatt aaccagatct tgttgctgtt
    36841 tctggtgaac ggacatatac cctaccgccg gagaggcgga ggccgggcgg cctgcataac
    36901 gcagagaagc cccaaacgga atcacttcac ggaaatgatg ctggctgcag taccatgcgc
    36961 cctggttgag cggctcttcc tggcaccaga caaaatcctt gacgtgagca aactgctgca
    37021 acacttcctg catcgcttta tgcgggaacg ggtagagttg ctcgatacgc acaatggcga
    37081 catcgtgttg attgttctta cgacgctgtt ccagcaggtc gtaataaacc ttaccagaac
    37141 acatcactac gcgcttcacg cccttcggat caagctcgtc gatttcaccg atggctggca
    37201 ggaaggtgcc gttcgccagt tcttcgaggc tggaaaccgc cagcggatga cgcagcaggg
    37261 atttcggcga catcacgacc agcggacgac gcatcccgcg cagcgcctga cgacgcagca
    37321 tgtggtaaac ctgtgccggg gtagacggta cgcaaacctg catgttttgc tcagcacaaa
    37381 gttgcagata acgttccaga cgcgcggagg agtgctccgg cccctgccct tcgtaaccgt
    37441 gcggcagcaa catcaccaga ccacacatcc ggccccattt ctgctcgcca gaggagatga
    37501 actggtcgat aaccacctgc gcaccgttgg cgaagtcacc gaactgcgct tcccagatgg
    37561 tcagggtgcg tggttctgcg gtggcataac catattcaaa cgccagcacg gcttcttctg
    37621 acagtacgga gtcccagaca cggaacgcgc cctgaccgtt atggatgtgt tgcagcggcg
    37681 tgtaagtgga accgttagac tggttgtgga tcaccgcgtg gcggtggaag aaggtaccgc
    37741 gaccggagtc ttcacccgac aggcgaaccg gaatgccttc atcaaccagc gtggcgtaag
    37801 cgaggttttc cgcaccgccc cagtcgaaca gtttctcacc ggctgccatc gcctggcgat
    37861 cgccataaat cttggcaacg cgagactgca tttcaactgc ttctggtacc gtactgatac
    37921 gtttagccag ttcctgcagg cgcttcatct caactttgtt cgggtactct tcgtcccatt
    37981 cgtggttgag gtacggcgac caggtgaaag agtgcatgtt catcggacgc cactctgcca
    38041 ctacgcaatc gccagcatcc agcgcatcgc ggtacaggtt aaccatctcg gtggcatctt
    38101 ccagcgtcgc cactttttcc tgctccagct tgtcagcgta gattttgcgc ggtgtcggat
    38161 gttttttgat tttctgatac atcagtggct gggttgcgct cggctcgtcg gcttcgttgt
    38221 ggccgtgacg gcggtagcat accaggtcga tgaagacatc acgtttaaag gtgttacgga
    38281 aatcgagcgc cagacgggtc acaaaggcaa cggcttccgg atcatccgcg ttaacgtgga
    38341 aaatcggtgc ctgaaccatc ttaccgatat cagtacagta cggtgtagaa cgggcatcca
    38401 gcgggttaga ggtggtgaaa ccaacctggt tgttgataac gatacgtacc gtaccgccaa
    38461 cttcataacc acgcgctttc gacatgttca gagtttcctg aaccacgccc tgcccggtca
    38521 ctgcggcgtc accgtggatg gtgattggca gcactttgtt gctgttcggc tcatcaagtc
    38581 tgtccagacg ggcacgaaca gagccgataa cgaccgggct tacaatctca aggtgagacg
    38641 ggttaaacgc cagcgccagg tgcaccaggc cgccatcggt ctggaagtca gacgagaagc
    38701 ccatgtggta tttcacgtcg cctgtgccga ggtgttcttt atgtttaccg gcgaactcgt
    38761 cgaacaagtc ttgcggtttt ttacccagca cgttcaccag cacgttcaga cgaccacggt
    38821 gcgccatccc gagaaccact tcgcgggtgc cgctgttgcc agcgtggcgg atcatctctt
    38881 taagcatcgg gattaacgcg tcaccgcctt ccagcgagaa gcgttttgcg ccagggaatt
    38941 ttgcgccgag gtaacgctca aggccttcag cggcggtcag ttcgcttaag aagcgttttt
    39001 tctcttcgct attgaaagtc gcgcgaccag actcgatacg ctgttggatc cagcgttttt
    39061 cttcggtgct ggtgatgtgc atatactcgg caccaatcgg gccgcagtag gtttgcttga
    39121 gggcggaaat aagctcgccg agtttcatgg tttctttgcc gctggcaaat gaaccgacgt
    39181 tgaaggtttc ctggaagtct gcttcggtca gatcgtggaa agacggatcc agatcggcca
    39241 ctttatcttg ctgccacagt cccagcggat ctagattcgc atgctggtga ccacggaagc
    39301 ggtatgcgtt aatgagctgc aggactttaa cctgcttcac attggtgtca gggtcggaga
    39361 tcgttgaaga gtaacgtgaa gcgtctttcg ccaggcggcg gaaatattca cgcgtttgag
    39421 agtggaattg atccggtttg actcccgtac caggtaactg ctggaacgtc gaacgccagt
    39481 tagcgtcaac cgagtcagga tcggttaaga agtcttcata gagctgttct atccagctct
    39541 ggtttgcgcc agagaggtaa gaagagtcca accaggcttt caaagcgctg ttctgcatcg
    39601 tgatccctta agcatctttt ttatgcttac ttcgccgtgg atactaccac gcacagtgat
    39661 gtgcgtgccg gggttcactt gcgagctctt gtctacgtag tggctcgcga aggaaccttt
    39721 aaaaactgtc ttagtgtcag gggcagtttt taaagatttc ctgcatttct ttgttacgcc
    39781 tgatgcgctg cgcttatcag gcctgcgggt aatgacttgt aggccggata aggcgttcac
    39841 gccgcatccg gcactggttg cctgatgcaa cgcttgcgcg tcttatcagg cctacagttt
    39901 acgcattacg ttgcaacaac atcgacttga tatggccgat ggcgcgcgtc gggttcagcc
    39961 ccttcggaca tacactgacg cagttcatga tgctgtgaca gcggaatacg ctgaatgcat
    40021 cgctcaaacc gtcgaggcgg ctgtcagtct cggtatcacg gctatcgatc aggaaacgat
    40081 atgccgctaa caagcctgcc gggccgataa acttatcggg attccaccag aaagacggac
    40141 aagaggttga acaacatgcg cagagaatac attcatacag cccgtcgagt ttttcgcgct
    40201 gctctggcat ctgtaaatgc tcgcgagctg gcggattttg tccattattc aacaggtaag
    40261 gcttaatttt ctcatattgc gcatagaatt gtcccatgtc taccaccaaa tcgcggatca
    40321 ccggtaaacc tggcagcggg cgaatcacaa tcttcttgcc cggctggttg agtgccgaaa
    40381 tcggggtaat acaggccaga ccattcttgc cgttcatgtt cagaccgtcg gaaccgcaca
    40441 caccttcacg gcaggagcgg cggaacgaca ggctgggatc tttctctttc agctggataa
    40501 gcgcatccag cagcatcatg tcgcgacctt cttccgcttc cagggtgtaa tcctgcatac
    40561 gcggagcatc atcaacatcc gggttatagc gataaattga aaactcgagt ctcattttcc
    40621 tgtctccgca ttagtaagta cgaatcttcg gcgggaatgc cgggcgcagt ttcggttcca
    40681 tgttgacgct tcggcgcgtc atggattccg actcaggcag atacagggag tggcacagcc
    40741 agttttcatc atcacgatcc gggaagtcga agcggctatg tgcgccacgg ctttcggtac
    40801 ggaagttggc agaaacagcc gttgcatacg ccgtttccat caggttatcc agttccaggc
    40861 actcaacgcg ctgggtgttg aactcgctgg aagtgtcatc cagacgggca tttttcagac
    40921 gctcgcggat cactttcaac tgctcaagcc ctttcgccat cgcatcacct tcacggaaga
    40981 ccgagaagtt atgctgcata cattcttgca gcgctttacg gatcgccacc ggatcttcac
    41041 cgttacgatt attgttccac cggttcaggc gatccagaga cgcttcaaca tcagactcgc
    41101 tggcatcgtg cagtgcgccc tgctcggcga tagactcttg cagatgcaga cctgccgcgc
    41161 gaccaaagac caccaggtcc agcagcgagt tgccgcccag acggttagcg ccgtgtaccg
    41221 atacacaagc gatttcacca acggcaaaca gtcccggaac aaccacatct tcgcctttct
    41281 cattcacagt cagcgcctga ccggtaactt tggtcggaat accgcccatc atgtagtgac
    41341 aggttgggat aaccggaatc ggctctttca ccggatcgac gtgagcgaag gtacgggaaa
    41401 gctccaggat acccggcaga cgggattcga gaacttcttt acccaggtga tcgagtttca
    41461 gtttcgcgtg tggcccccac ggaccatcac agccgcgacc ttcacggatt tcaatcatga
    41521 tggaacgcgc caccacatca cggcccgcca ggtctttggc gttcggcgca taacgctcca
    41581 taaagcgttc gccatgtttg ttcagcagat aaccgccttc accacggcaa ccttcggtga
    41641 ccagtacgcc cgcaccggca atgccggtcg ggtggaattg ccacatttcc atatcctgca
    41701 ccggtacgcc tgcgcggata gccatgccga caccgtcgcc agtgttaatg tgggcgttag
    41761 tggtggactg ataaatacgc cctgctccgc cagtcgccag cacggtagcg cgggctttga
    41821 aataaaccac ttcaccggtt tcgatgcaca gtgcggtaca acccaccacc gcgccatcct
    41881 ggtttttcac cagatccagc gcataccact cggagaaaat ggtggtgtgg tttttcaggt
    41941 tctgctgata aagcgtgtgc aacagtgcgt gaccggtacg gtcagcagcc gccgcagtgc
    42001 gtgccgcctg ctcgccgccg aagtttttcg actgaccgcc aaacggacgt tgatagatac
    42061 gaccatcatc gagacgcgag aacggcaggc ccatgtgttc gagttccaga atcgcttccg
    42121 gcccggtttt acacatatat tcaatcgcgt cctggtcacc gatatagtcc gaccctttta
    42181 cggtgtcgta catatgccat tcccagttat cttcatgggt attacccagc gcaacggtaa
    42241 tgccgccttg cgcagaaacg gtatgggaac gggtcgggaa gactttagag agcagggcac
    42301 aggtctggcc gctctgggaa atttgcagcg ccgcgcgcat acctgcgcca ccggcaccaa
    42361 tcacaactgc atcaaattct ctgactggca atttcatcac acaccccaca ccacaacgaa
    42421 tccataaatc acgtaaacca ccagtgcaac gacaatcacc agttgcagca tcaggcgcaa
    42481 ggccagcggt ttaacgtagt cggtcaacac ctgccacatg ccgatccagg catgaatcaa
    42541 gatagaaaac agcgccagca gggtgaacac tttggtgaac gcagaggcga agaaaccgat
    42601 ccagacttca tatgtcagtt cgccactggt ggcgaaaaaa ccgaccatat aaatgatgta
    42661 gagcgtcagg acgatagcgg tagcgcgaac gaggatgaaa tcatgtacgc cattgcgtcc
    42721 taatgcggag gcgttgctta ccatacgagg actcctgcga gaagtgaaag cacgacagta
    42781 ataacaaagg agattttggc ggagcgttta cccgcttcga atgtttcttc cagatagcca
    42841 aaatccatca tcatgtggcg aatacctacg acgacgtgat acgccagagc ggtaaggatg
    42901 ccccacatga taaatttgac gaagaagctg cccataatcg cggaagcttg ctcgaaacct
    42961 tcaggggaag agaggctggt acccagaagc cactgcagga tgcccactgc aacaaaggtg
    43021 atcacaccgg aaacgcgatg gagaatggac gctatcgccg tgatggggaa ccggatggtc
    43081 tgtaggtcca gattaacagg tctttgtttt ttcacatttc ttatcatgaa taacgcccac
    43141 atgctgttct tattattccc tggggactac gggcacagag gttaactttc tgttacctgg
    43201 agacgtcggg atttccttcc tccggtctgc ttgcgggtca gacagcgtcc tttccataac
    43261 tgcgcgtcat gcaaaacact gcttccagat gcgaaaacga cacgttacaa cgctgggtgg
    43321 ctcgggattg cagggtgttc cggagacctg gcggcagtat aggctgttca caaaatcatt
    43381 acaattaacc tacatatagt ttgtcgggtt ttatcctgaa cagtgatcca ggtcacgata
    43441 acaacattta tttaattttt aatcatctaa tttgacaatc attcaacaaa gttgttacaa
    43501 acattaccag gaaaagcata taatgcgtaa aagttatgaa gtcggtattt cacctaagat
    43561 taacttatgt aacagtgtgg aagtattgac caattcattc gggacagtta ttagtggtag
    43621 acaagtttaa taattcggat tgctaagtac ttgattcgcc atttattcgt catcaatgga
    43681 ttctttacct gcaagcgccc agagctctgt acccaggttt tcccctcttt cacagagcgg
    43741 cgagccaaat aacaaacggg taaagccagg ttgatgtgcg aaggcaaatt taagttccgg
    43801 cagtcttacg caataaggcg ctaaggagac cttaaatggc tgatacaaaa gcaaaactca
    43861 acctcaacgg ggacacagct gttgaactgg atgtgctgaa aggcacgctg ggtcaagatg
    43921 ttattgatat ccgtactctc ggttcaaaag gtgtgttcac ctttgaccca ggcttcactt
    43981 caaccgcatc ctgcgaatct aaaattactt ttattgatgg tgatgaaggt attttgctgc
    44041 accgcggttt cccgatcgat cagctggcga ccgattctaa ctacctagaa gtttgttaca
    44101 tcctgctgaa tggtgaaaaa ccgactcagg aacagtatga cgaatttaaa actacggtga
    44161 cccgtcatac catgatccac gagcagatta cccgtctgtt ccacgctttc cgtcgcgact
    44221 cacatccaat ggcagtcatg tgtggtatta ccggcgcgct ggcggcgttc tatcacgact
    44281 cgctggatgt taacaatcct cgtcatcgtg aaattgccgc gttccgcctg ctgtcgaaaa
    44341 tgccgaccat ggccgcgatg tgttacaagt attccattgg tcagccattt gtttatccgc
    44401 gcaacgatct ctcctatgcc ggtaacttcc tgaatatgat gttctccacg ccgtgcgaac
    44461 cgtatgaagt taatccgatt ctggaacgtg ctatggaccg tattctgatc ctgcacgctg
    44521 accatgaaca gaacgcctct acctccaccg tgcgtaccgc tggctcttcg ggtgcgaacc
    44581 cgtttgcctg tatcgcagca ggtattgctt cactgtgggg acctgcgcac ggtggtgcta
    44641 acgaagcggc gctgaaaatg ctggaagaaa ttagctccgt taaacacatt ccggaatttg
    44701 ttcgtcgtgc gaaagataaa aatgattctt tccgcctgat gggcttcggt caccgcgtgt
    44761 acaaaaatta cgacccgcgc gccaccgtaa tgcgtgaaac ctgccatgaa gttctgaaag
    44821 agctgggcac caaagatgac ctgctggaag tggctatgga gctggaaaac atcgcgctga
    44881 acgacccgta ctttatcgag aagaaactgt acccgaacgt cgatttctac tctggtatca
    44941 tcctgaaagc gatgggtatt ccgtcttcca tgttcaccgt cattttcgca atggcacgta
    45001 ccgttggctg gatcgcccac tggagcgaaa tgcacagtta cggtatgaag attgcccgtc
    45061 cgcgtcagct gtatacagga tatgaaaaac gcgactttaa aagcgatatc aagcgttaat
    45121 ggttgattgc taagttgtaa atattttaac ccgccgttca aatggcgggt taatttttat
    45181 atgcctaaac acaaaaaatt gtaaaaataa aatccactat cggacattca tatattctaa
    45241 aaaaaaatac atcgatataa attataaaca aatagatgat ttttaatcat ctatcttcct
    45301 ctcagttaca tgaataaacc accaaatata gcgcacccct ataattccca atactttatt
    45361 catcaaacat ttaattcata ccatcaatta attactttta catttaataa aattaataat
    45421 atttttaaga aaaactaaaa acattcgaca atcaaataag atattcttgc accgatatac
    45481 ttttttcttt tctgttaaag gactcagtta tgtttaaagg acaaaaaaca ttggccgcac
    45541 tggccgtatc tctgctgttc acagcacctg tttatgcggc tgatgaagga tccggtgaaa
    45601 ttcactttaa aggtgaagtt attgaagcac cgtgtgaaat acatcaggat gatattgata
    45661 aagaggttga actcggtcag gtgaccacca gccacattaa tcagtcacat cacagcgatg
    45721 ccgttgctgt cgacctgctc ttagtcaact gtgatctgga aaactccagc aacggttccg
    45781 gtggcaagat ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc accggcgcag
    45841 atccgattct caacaacacc agcacgggtg aagccaccgg cgtcggcgta cgtttaatga
    45901 ataaagatca aagcaatatc gttttaggca ccgctactcc agatatcgac ctggctccga
    45961 cctccagcga acaaacgctg aatttctttg cctggatgga acaaattgat caggctacac
    46021 ctgtaacgcc aggcgcagtt acggcgaatg caacctacgt gctggattat aaataagtat
    46081 tactttggcg gcgctatcgc cgcctttcat aaaatattta tcaggtatgg acaccgtgaa
    46141 tatttatcga ctctcttttg tatcctgcct ggtcgtggcg atgccttgcg cattggcggt
    46201 cgaattcaat ctgaatgttc tcgataaatc aatgcgcgac cgcattgata tttcattatt
    46261 aaaggaaaaa ggagtcattg ctcccggtga gtattttgtt agcgttgcgg taaataataa
    46321 ccaaatcagt aacgagcaaa agattaactg gcacaaaaat gacgataaaa ccattccgtg
    46381 catcaatgat ttactggtcg ataaatttgg cttaaaacct gaagtccgtc agtcgttacc
    46441 attgataaat cagtgcgtcg attttagctc ccgacctgaa atgctcttca atttcgatca
    46501 agccaatcag caactgaata ttagtattcc gcaagcctgg ctggcgtggc actcagaaaa
    46561 ctggacccca ccctccacat ggaaagaagg tgtcgccggt gtcctgatgg attacaactt
    46621 gtttgccagc agctaccgcc cacaggatgg cagcagcagc actaatctta acgcctatgg
    46681 aacagccgga atcaacacgg gggcatggcg cttacgcagt gattatcagt tgaatcatac
    46741 tgatagcgat gataaccatg aacagtcagg cgaaatatca cgtacctatc tttttcgccc
    46801 gttaccgcaa ttaggttcaa aattaaccct cggcgaaacg gattttagtc ccaatatttt
    46861 cgacggtttt tcttataccg gcgcggcact ggcaagtgac gatcgaatgt tgccatggga
    46921 actacgcggc tacgccccac aaattagcgg tattgcacag accaatgcca cggtgacgat
    46981 cagtcaatca ggccgcgtca tttaccagaa aaaagtccca ccaggcccat ttatcattga
    47041 cgaccttaat cagtctgttc agggcacact ggatgtcaaa gtgacggaag aagatggtcg
    47101 ggtgaacaat ttccaggttt cagcagcatc gacgcccttc ctgactcgtc agggacaggt
    47161 tcgctataaa ctggccgcgg gtcagccacg gccctccatg tcacatcaaa ctgaaaatga
    47221 aacctttttt agcaatgaag tttcctgggg gatgctgtca aacacctcgc tgtacagcgg
    47281 cctgctgctt tctggtgatg actaccattc tgccgcaatg ggtattgggc aaaatatgct
    47341 gtggcttggt gcgctgtcgt ttgatgtcac gtgggccagt agccattttg atactcagca
    47401 ggacgagcgg ggcttaagct accgttttaa ttacagcaaa caagtggatg ccactaacag
    47461 cacgatttcc ctcgccgctt atcgtttctc cgatcgtcat tttcacagct acgccaacta
    47521 tctggatcac aaatacaacg acagcgatgc gcaggacgaa aaacagacga tcagcttatc
    47581 tgtgggccaa ccgattaccc cactaaacct caatctttac gccaacctgc tacatcaaac
    47641 ctggtggaat gcagacgcct ccacgaccgc caacatcacg gcaggtttta atgttgatat
    47701 tggtgactgg agagatatct ctatttctac gtcattcaat acaacccatt acgaagataa
    47761 agatcgcgac aaccagattt acctgtcgat ttcgctcccc ttcggtaacg gtggtcgggt
    47821 tggctatgac atgcaaaaca gtagccacag caccacacac cgcatgtcgt ggaacgatac
    47881 gctggatgaa cgtaatagct ggggcatgtc tgccggactg caatccgacc gtcctgacaa
    47941 tggagcccag gtgagcggta actatcagca cctgagttca gcgggtgagt gggatatttc
    48001 tggtacctat gccgccaatg attacagttc cgtcagcagc agctggagcg gttctttcac
    48061 cgcaacccaa tatggtgcag cgtttcatcg ccgcagctcc accaatgaac cacgcctgat
    48121 ggtcagcacc gatggcgtgg cagatattcc ggttcagggc aatctcgact acaccaacca
    48181 ttttggcatt gcggtggtgc cgttgatttc cagttaccag ccttccaccg tggcggtgaa
    48241 catgaatgac ttacccgacg gcgtaacagt tgcagaaaac gttatcaaag aaacgtggat
    48301 tgaaggcgcg ataggttaca aatcactggc ttcccgttcc ggtaaagacg ttaacgtcat
    48361 cattcgcaac gccagcggtc agtttcctcc cctcggagcg gatatccgcc aggatgacag
    48421 cggcattagc gtggggatgg ttggcgagga aggacatgcc tggttaagcg gagtcgctga
    48481 aaatcaaaag tttaccgtgg tctggggtga tagccagcat tgctcgctcc atcttcctga
    48541 acatatggaa gacaccgcaa atcgcctgat tttaccttgt cattaattaa ggaaatgaaa
    48601 atgacattta tgaaaggact accccttttg ttgttggttg ccaatctgtg cagccacgct
    48661 gcactacaac ccgatcgcac tcgtattgtg tttaacgcta atgataaagc taccagtctg
    48721 cgggtggata atcgtagcga taaactcccc tacctcgctt attcctggct cgaaaatgaa
    48781 aaaggggaga aaagcgatga tcttctggtc gccctgccgc cgatacaacg tctggaaccg
    48841 aaagcaacaa cacaggtacg gatagtaaag caagcctcga ccacaaaatt accgggcgat
    48901 cgcgaaacgc tatttttcta caatatgcgt gaaattcctc ccgccccgga aaaaaacagc
    48961 gaccatgccg ttctgcaagt cgccattcag agtcgtatca aggtgttctg gcgaccggct
    49021 gcattacgca agaaagcagg agaaaaggtc gaactgcagt tacaggtcag ccagcagggc
    49081 aaccaactga cactgaaaaa tcctaccgcg tattatctga ccattgccta cctgggacga
    49141 aatgaaaaag gcgttctccc cggcttcaaa accgtgatgg ttgcgccttt tagtacggtc
    49201 aacacaaaca ccggaaatta tagcggcagc cagttttatc tcggttatat ggatgattac
    49261 ggcgcgttac gcatgacgac gctcaactgc agcggacaat gccatttaca agccgtggag
    49321 gcgaagaaat gagtgctggc aagggagtgt tgctcgtcat ctgtctgtta tttctgtcgt
    49381 tgaagtctgc gcttgccctg aactgctact ttggttcatc cggtggctcg gtagaaaaat
    49441 cagaagcgat tcaacctttt gctgtaccag gcaatgccaa actcggcgat aagatctggg
    49501 aatctgacga tattaaaatt cccgtctatt gtgacaacaa taccaacggc aactttgaaa
    49561 gcgaacatgt ctacgcatgg gtaaacccct acccgggggt gcaggatcgt tattatcaac
    49621 tcggagtaac ttacaatggc gttgattatg acgccaacca gggaaaaagc cgcatcgaca
    49681 ctaaccagtg tatcgacagt aagaacattg acatttatac ccctgatggg atggcagaac
    49741 aaaatttgct cgggtgatcc cgctaatatt cacatgtcgc gaacctttct cgctcgcatg
    49801 cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac
    49861 tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagaccctac tgcccaaaac
    49921 ctgaaatatc atattactgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt
    49981 tccatttcac cggaaacaca gatgattgat tttggtaaat ttaatttgct ggatattcgt
    50041 cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc
    50101 accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat
    50161 aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct
    50221 tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa
    50281 aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc
    50341 gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc
    50401 ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt
    50461 ttttcctagc ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga
    50521 gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat
    50581 tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat
    50641 catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca
    50701 ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc
    50761 cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt
    50821 gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct ggggttcttc
    50881 tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct
    50941 ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac
    51001 ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc
    51061 agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta cggcgggatg
    51121 catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca
    51181 gtctggtcag ctcatcacca tcgaactacc ggaatggctg ctggcgatgg cgtatatggc
    51241 aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc
    51301 gctaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcag gtatggcgtg
    51361 gggactgacc cggtttatgc atattgattt tatgactgcc tacctcgcca ccagtcccgg
    51421 cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat
    51481 ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca ttgcgcggtt
    51541 tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac accagtaaaa
    51601 cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc acagttcgcc
    51661 atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg
    51721 cccccgcgta gcgtaagaaa accgaggaat ctccagtaac gcgtgtgcga gtgcatccag
    51781 ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct gccagaggat
    51841 ctccacccgc aaataattgc caagcccagc cagaaacgcc tgatcgagca gtaatcctgc
    51901 aaactgacgg ttacgaaagc gcggcgacaa taaccgttcc tttaccacct ccggcgtcag
    51961 attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc
    52021 cggcctcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg
    52081 cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca
    52141 gacgccgtaa agctgattat ggctgtagag cgttaaaccg ttgggaaaat gagttaacaa
    52201 cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt
    52261 ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc
    52321 caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca
    52381 ttctttatta agcggggtta tgctgcgaca acaatcccct ttttggcaaa tgtagctcgt
    52441 aatcggcggg cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac cgtttgcgcc
    52501 gcgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctccagc
    52561 gtttgcgcca acgcctgttc ttcgttttca atcaacgcgc ctggctgact tcgcggcacc
    52621 agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca
    52681 tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaac caatgccgga
    52741 tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg
    52801 ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc
    52861 gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttccgg cggcagctgc
    52921 atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aatcgcgaca
    52981 ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc tgcatgaaat
    53041 ccacaagcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct
    53101 tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacgcta attgttcaaa
    53161 ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg
    53221 aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa tgatacaggc
    53281 aatacgtgga taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg gctgcccgtt
    53341 atgcggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcgc gatcggtgat
    53401 gcgttttaaa atttgcccct gtaaacgata gcccattcgg ttactttgcg agctaagctg
    53461 ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc
    53521 cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa
    53581 atcacgcttc gatttgccaa tcgggagtca gtcaccatcc ttcagtaaac ggccttccag
    53641 cccgccaatc cccactttga gatcggtgct gcatgacccc attaccggcg gaacatcaat
    53701 accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg
    53761 ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcgttatcat ccaaccgcgc
    53821 ttcgcaacct gcacccgtca gggcaaacca cccatcagtt tcgaactcaa ctgttaactg
    53881 accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagca ggttagcaat
    53941 gcgcagcgca ggcatatcca gtgcgccaca gtggctgata cccgactggc aaaaaccgtg
    54001 acgaccgcca tcctgcacag tggtgtacat gcccgcacga ataatcttca gcatattccc
    54061 tccttctgcg gtacaaatcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc
    54121 gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc
    54181 agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagcaag
    54241 cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag
    54301 cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc
    54361 tgtttttcgc tcaacccgca atacgccgcg accaccgcca aatccggtcc gcctgcacca
    54421 ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc
    54481 caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc
    54541 accgtgatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc
    54601 tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc
    54661 actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat
    54721 tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt
    54781 cagccactcg ctcaatgcgc gaataccacc gcgttcagtg gcatggtgac ctgcagcata
    54841 aaaatgcaat ccctgctcgc gggctgaatg aatggtctgc tcagaaactt cgccagtaat
    54901 aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca
    54961 ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc
    55021 cagacgcgct tcaatccagg aggctagttc cattcccggc acaggcatgg tcagctctcc
    55081 ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg ccgccagctg
    55141 tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat
    55201 atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc
    55261 tcctttccag aagtagccat gatgcacaat gaccgcatct gcgcccagac gcactgcctc
    55321 atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc
    55381 ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt
    55441 aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca
    55501 ccgttaacat agcgttacat cgcctgttcg tcgatagcca atcttttgac atattaagaa
    55561 acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat
    55621 ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta
    55681 aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt
    55741 atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct gcaaatctgg
    55801 gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta
    55861 aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc
    55921 acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg
    55981 ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga gatccatccg
    56041 tcattcctct atctgtccct ggcgattatc gtctgcggct atggcctgtt taaatctaac
    56101 gtaagctgtc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc
    56161 tcgctgatgt atgcggcggg taacgtgggg tctattatcg cacctatcgc ctgtggttac
    56221 gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggttgg catgatcgcg
    56281 ggtctggtca ttttcttatg tggcaatcgt catttcactc atacccgcgg cgttaacaaa
    56341 aaagtactgc gtgcgacaaa ctttctcctg ccgaactggg gatggctgct ggttctgctg
    56401 gtggcaacgc ctgcactgat taccgtgctg ttctggaaag agtggtcggt atacgcctta
    56461 attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac
    56521 cagaaacagc ggaaggagct ggggctgatt gtgacgctca ccttcttcag tatgttgttc
    56581 tgggccttcg cacaacaggg cggtagctcg attagccttt atatcgaccg cttcgttaac
    56641 cgcgatatgt ttggttatac cgttccgacc gcgatgttcc agtcgattaa tgccttcgca
    56701 gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt
    56761 accgtgcgca tctggggaaa atttgctctt ggccttggcc tgatgagcgc cggattctgc
    56821 attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct gccactgatg
    56881 gtgttaggcc tcgcggtgat gggctttgcg gaactgttta tcgacccggt tgccatgtcg
    56941 caaattacgc gcattgaaat tccgggcgtg accggcgtat taaccggcat ctacatgctg
    57001 ctttctggcg cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac atcgcaggct
    57061 tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga agtatttgat
    57121 caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag
    57181 gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aataggccag caccgatgcc
    57241 tgatgcgacg cttgacgcgt cttatcaggc ctacgtattt cctgcaattt attgaatttg
    57301 cacaaatttg taggctggat taggcgttca cgccgcatcc ggcatctggg ctcgattgcc
    57361 tgacgcgtct gttatttccc cttccgcgcc gcctcatacg ctgccaacgt ttgtactctc
    57421 gcctctttgt gctcgactat cggttgcgga taatccagct tcacacctgc tttctgcgcc
    57481 cacttccacg gctcatgcac cgctttccct ggtacatcgc gcagctccgg taaccatcga
    57541 cgaataaact cgccctcacg gtcaaatttc tcgccctgag ttatcggatt gaaaatacga
    57601 aaatacggcg cagcatcagt acccgtagaa gccgcccact gccagccacc gttattagct
    57661 gccaaatcac catcaatcag ctgcgacatg aaatatcgct cgccttcgcg ccagtcgatc
    57721 aacaaatctt tcaccagaaa actggctgta atcatccgta gccggttatg catccagcca
    57781 gtgctgttaa gctgacgcat agcggcatca ataatcgggt atcccgtttt gcctttctgc
    57841 caggcctgta aatgtgcggg attgctttgc cactgcacac gatccgtcca ggcaataaat
    57901 ggacgatgtt tacacaacga ggggtaatac gtcatcagat gacggtagaa ctcgcgccag
    57961 atcagctcac taagccagac actaccggcc ccaccgtcca gcgcctgcgg ctgttccgcc
    58021 aacaagcgat gcaaacactg gcgaggcgat aacccgcccg ttgccaagct ggcggacaaa
    58081 cggctggtgc cttccactgc cggaaaatct cgttgttgct catactctcc agcaccgttc
    58141 tcgcaaaact ggcgtaattg cgcaatcgcc gctttttctt ccaccgggaa atgcgcagta
    58201 tcgaaagact gacgaggata attcagcgta atggatggcg cgggctttat cgatccgcta
    58261 ctacgaactt ttggtgcagc gacgcactcc ggcatccctt cccgcagccg tttcagccag
    58321 gcattcttaa aaggcgtaaa gactttgtac atctcgtgat tgccggtcat caccgccccc
    58381 ggcggcagga tcacgctgtc atcaaatcct tcacacacca cgttacgcag cgctctttca
    58441 acgtccacat cccgcgctcg ctcattcacc tcatactgat agttataaaa aagatgagta
    58501 acgctgtttt ccgcgcacac ctgtttaact atttcgacac tggcgacaaa gtcatccact
    58561 tcacggaaca ataaaggaat acctttttcc gccagcgcga tttgtagccc attcagttga
    58621 gcattgataa gttcagcctg acgcggcgac atgttatgcg tctcccactg gcgtggtgta
    58681 gcgatataca gcgccagcac gcgtgcagac gaattgcggc aggcggcagc cagtgcgaga
    58741 ttatcgtgca gacgtaaatc ctggcgaaac caggccagat gggtagtcat aaaactcctg
    58801 aatggcgcaa gttaatcaca cgcggccaaa gttcgaaata acgctgccct gaaagccagg
    58861 cgtcaggata aagcgccata tagtgtttga tacgcatcag cggcgcaaga agtggttgct
    58921 cgccacggcg ataactgtcg ataagcgcag ccagctgctg gcgttctgtg gaatcaatat
    58981 gcgggcgaaa ataaccctga acgtgcatca aaacgttggt gtgatcgcgc gggttggcgg
    59041 gatgagaaag caaaacaatt acgcgttggc ggtactggtt gtaaaaatca tccagatttt
    59101 gccactgatg aatatcagcc acaaacgggc caagtttgcg gtactcgggc tgagaatggg
    59161 ccagaaagac gagtttatag cggctgtgat aatcgagaag cgccccgcgc gttaagccat
    59221 gttctttcag tcgatgtaac tcatgaaggg cgaagatacg gattagggtg ctgtcatcaa
    59281 atcgttgtag attcattact gctcctgaac ggtatacagg agtaagtgta ggcgttggta
    59341 aatgatgttt gtgaagttaa tccgctagca gggcaagatt acgaaagtgc gctccccgca
    59401 agcaactaat caaggtagtt aatcatggct gaacacgata ttacgccaga agtcaccatt
    59461 tccaaaacac agcgccgcta taaagtcggc tatgtcagag cccgccacca ggaccgcaaa
    59521 accggtatag cccggtatta ttcacaacat cccagcctgc accttaaggg cgactggctg
    59581 aaagaggccg gatttgatac cggacggggt gtcacggtga agatttcgga ggggggcctg
    59641 accatcatcg ctgacagtga cgaggtgcag gaactgcggg atgggatgtt tagtgtgctg
    59701 aacgaaagtt aaatattaaa aaagctggga agatttaaaa ccttcctagc tttttattct
    59761 gtcttattat aatggatact catcatcttc cgatgaattt ataaattgct ccaagtttac
    59821 atgaagagta cttaacctag tttttaactc attaagtgaa gcgataattt catattttcc
    59881 aacataatca tatgatggat cgtattcatt atctatgtca gaagctaaaa atgatgatat
    59941 tcgtagttta aatttagctt catctttatt acattcattg gcataaggtg aatgccctat
    60001 catagcatat tcatctaatc taaaaggata agatggtttt aattcatcga tcaatgaaat
    60061 ttcaatccat gttctatatc caggcattaa tggactaatt tctttgagca aaacctttcc
    60121 acttaataat gtattgagat tgttcattat tatgtctccc acgggaaaaa tatatgtcta
    60181 acacttccac catgttgatg aacttgtaaa tgtggtaaat caccatcagg atttcctggt
    60241 ccatgacctg acactctata tgaaggtttt gttacagaag gatcttttat atcatcccaa
    60301 tgatatgaac ctgcttttgt tttatttcca gtaagatatt ccattaagtt cttagtgctg
    60361 gggcctgact cacctgtcat atttttataa ttatgaccta aatatctatc cataaacaat
    60421 tgttcagcgt cacttctaga cctaacagta actttggttt cactactatc taataaacga
    60481 ttcgcttttt ggttactaac agcacatgga gttaatccaa gtggatcagc atattttaga
    60541 ggattaggtg cataactgta gagatttaaa ccgcctctca accctatcgg gtcagttacc
    60601 atgaaccgcc cgacatccgg atcatagtac ctgtgcagat tgtagtgcag ccccgtttcc
    60661 ctgtcgaggt actgtccctg gtagcgcagg ttctgctcca ccccgccgtg gtccttctcc
    60721 tgtatcgtgt taccccagac ctgatacccc gtctcccaca cgattttccc gtcgctgtcc
    60781 gtcatctctt ccggcgcgcc gttcacatcc gtgtgaaaat acagtatccg gttcgggccc
    60841 tctttgcccg ccttatccac ccgcgccagc ggttcataac tcccctggtc cctgtagatg
    60901 tacaggctgc ctgttccctc cggcctgctc tcctgcgcca gccgcattcc gtcccataaa
    60961 aattttgtcc ggttgtacgg cttaccgtca gggctgatgt gctgtttttc cgtccgcctc
    61021 cccagtgcat cgtacacata ccggtatgtc ccccgcgctg tcgtcacttc caccatccgg
    61081 tgttccgcat cataacggta actctgcgtt ccgctccggc cctccttttc caccgcacgg
    61141 ccaaattcgt cataacggta gtgcacacca cggtaagaca tcagccggtt gaacggcacc
    61201 acgctgtttt ctgccgtggc cctttccccc gcgcgcctcc ccagcaggtt tcccgccgcg
    61261 tcgtactgcc agctcgccag atacccctca tcctccgaac gacacgccgt gatgcgcccc
    61321 gtggggtcgt agaagtaatc cgtttccccc cgccggctgt gccgcgtctt cagcagttca
    61381 tcctcgccac tgtagctgta ctgccggtcc gtgaacgtct ccggtaccac cccgcgcatc
    61441 ccgctgtatg tctccctgcg cttcagccgc ccgccggggt cgtaaagctg ccgcgtgctc
    61501 agtcgcccct ggcttctgag cacttcacgg tgcaggtggt cgcgcgtgaa ctcgctgatg
    61561 acctcccggt ccagatttat ctgctggaga tggccgctgc cgtagtacag atggcgctgc
    61621 gttctgccgt ccggaaaggt ggtggcagtc cggttgccca gtttatcgta ctggtgggtc
    61681 aggatgccct gcgccgtctc ctcgctcagc atccgcccca gtctgtcgta gctgaagcgg
    61741 ataacttccg gcgcgtcagt ttcgtgccgc tggcgacgga tttccagtag ctggtctgcc
    61801 gcatcatgga cgtattccgt acggccatcg gctgtctctt ttgccgtgag ccggcccgcc
    61861 gcatcacgcg ccagccggtg gcggatttca ccgccccggt ccccggtgaa caccacttcc
    61921 gtgaccgcgt tcagcgcgtt gtagcggtac gactggcggc agccgtccgg gtttatctgc
    61981 tccgccagac ggtccagcac atcatggcgg aaccggtagc gttctccgtt ctcgttcgtg
    62041 agcgtgacca gacgcccgta agcgtcgtac ccgaatgccg tcctgcgccc catcgcgtcc
    62101 gtccggctgc gcacccggcc ctgcccgtcc cgctgccagc gcgttatcct ccccatcggg
    62161 ctggtgtact ttaccagcct gcccgccgcg tctgactggt aattcaccgt gcggccgtcc
    62221 gccagtatca cctccgtcag atgaccgctg tcgtcatacc cgtagcgcgt gctgtggctc
    62281 tgcgcatccg tcattctgat aagcctcgtg cgttcgtcgt agaaccacgc cgtctgcgag
    62341 ccggaacagt ccgtgtgccg catgagctgc ccgtcttcat tccactggag gtatttattc
    62401 cccccgcgcg catccgttat ctgcaccacc tggccgtggt ggtcatactg ataccgggtg
    62461 ttctgctgca gcgggtcggt cacggcaaga aggttgccgc gttcatcgta cacacagcgc
    62521 caggtgtttc ccgccgcgtc cacctccgtt tccggctggt gccacaccgg gtgccactgc
    62581 gcctgctcca cccgccccag cgggtcgtgc gtctccgtga tatgcccctg caggtcatac
    62641 acatagcgcc atttgccgcc ctgagggtcg gtcatcccca gcaacagtcg ttcttcgtcg
    62701 ctccagcgga atgtcgtcac ctgtccggct tcgtcctggt acgccgtcac ctgattctgc
    62761 gcatcccact ggtgataacg tgtgcgcccc agtccgtccg tcacgcacag cctgcgctgc
    62821 gccaggtccc acgccagccg gtactgttca ccgctgtccg tgtggtgccc cgtcacccgc
    62881 catgccgcgt catccggacc ttcataccag ccccactggt aataataccc ttcgccgccc
    62941 ggctggcggt gcagcgtcat gcagtggttt tcgtcgtaag caaactcacg cagcaggtgc
    63001 cccgtgctgt cgtacacgcc gttcaggtgg ccggcctcgt catagctgta acgacgcagc
    63061 agtaaaggtg cagtctcagg gaaatgctga taaatctgtg tcacccgccg gggatgcgcc
    63121 gccagttcgt aatacagacg gatgcacgga cgctgctgtt cgccgctgat ttccacaatg
    63181 cggccccggt cgtcgtagta cagattcaga cagttgctgt tacggtcgcc cagcattttc
    63241 agccgctggc gctgcgggtg gtgcgggtca gcctcaaaca gaaaacactg cccgtcatcg
    63301 ctgctgataa gccagtgccc ctgctcacca cgccttaccg ccagcccttc accggggctg
    63361 taaaacccgc cccccggcag tacggcttcc atatccaagc gtcgccccat gcccgacaca
    63421 aaggtcatgc agttttcgtc tgcgctaccc ggtgtgcaca cgaggctgac ttcatacatt
    63481 acactccagc cctgaccaaa cattccttcc gtacgttcgt cacgactgct gtacacgcgc
    63541 tgccagtcca gcggaaagtg gcccggcaga ctgaaatcga agtcatcgtc atcggccagg
    63601 tatttggccc cggtggcggc attgaacgga tgcggaaggg cactggtggc cttctgcgtc
    63661 agcatgctca tcccgacact ggcagcaaaa caggcgattt tagaaagcat tttgcccggg
    63721 cgaagaaaca tcagcgccac ggttgccacc agggttatct gcgacctgcc gcttctgata
    63781 tccctgacca ccagtaacgg accgccaatg cgcacatccg gtgacacaaa cgccccgttc
    63841 cccggctcat tcaccaccct ggcctcgcag gtacagcgtg ccccgctgcg tgtcgccggc
    63901 tggctgttga tacgcacatc ccgcgcgcct tccgcgatat attccgctgc gggcggctcg
    63961 tcatcgcctt tgaacagatt actgatgctg ccaataaccg cattcactcc ctgaaagacg
    64021 gtttccagca taccgcccgg attcagcagg ttaagggccg ttcccagggg ggcatccaga
    64081 aaggcggctt ttttttgtgc gagaaactgc gtcagtgagg aaggatgttt atggcagtcg
    64141 attttatcgt caggggtcgg ggtggatccc ggggccgcac tggccacagt gggctgccat
    64201 agttccgaca caaaatgtgt cacgccatcc accatttcag aacgcccttc ttccgtactg
    64261 agctggtcgt gcagagcccg ggacatcagc acaggccctg tcaccgggtt aaccgccagg
    64321 gctatggccc ccgccatttc cagatattcc ccggccacat cccggaacgt tctggcatcc
    64381 tcctgacgct gcgcttcttc ctgttctgcc ttcgcctgcg cttcctgcgc ctctatgtcc
    64441 tgccgcgatg ccgcagtcgc cgctgcccgc gcggcaggtt tgctgttgat ccatgtgtca
    64501 ccagagccgg tgaggatgta accttcaatc tgaggcggga aaagcatatt tgcgagggct
    64561 gaacaggctt cgctgacctt ttctgtcacg ccggtgtaca ccatcgccac acccgccaca
    64621 aaaccggtca gcacgacggc cgtgcacccc atcgtgatcc ccactgacgt cgccagtaac
    64681 gaggttgtgc cgacaatgag ggcatcaata acgacatttg cggccacctc cagcaccgcc
    64741 cccagcacat ctgccataaa cgacgtatgc agcagggcat caccctgtct tgccgcccag
    64801 aatgcctctt ccatgtcccg ttccctgtat gattaattcg tcctgacttc ctgccgcatc
    64861 ctgaagcgca gattcttttt aatttcagcc cagcgcgtca tatctgccgg ggtcagcggt
    64921 gtggttctgg cgtaggtgaa ggccatcatg acccgctcac ccggcaatac aaatgccacc
    64981 tgacactggt acacctgctc gttaccccgg agaaactggc tctgtatttc caaaccgcct
    65041 cgttgagata atattccgtc ataatgtaaa tagtccgttt taataaagga attatttatt
    65101 gacaggttct gtttttttaa tcatgaatgt aatacccatc accgctttac agcacacttt
    65161 atctttccag ccataatcac taataatatt aagcaattag caatatttac aacgaccaga
    65221 gcggatacat agttatgtat ttaacaatcc caatatattc actatgcaaa taaaaccact
    65281 agtaacagac acgctaccgc ctccatataa aaatggacac cttataaaat tgtatcttgc
    65341 atagaaaaag ctgaaatatc ggatgcgttt taattatcaa ctataaatct ataagctttt
    65401 tattaacaac aa
//
LOCUS       DAJTRB010000009        65161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTRB010000009 DAJTRB010000000
VERSION     DAJTRB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.009"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO330_000644"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO330_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5881631.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1152..1238)
                     /locus_tag="OO330_000645"
     CDS             complement(1152..1238)
                     /locus_tag="OO330_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5881632.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO330_000646"
     CDS             complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO330_000646"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5881633.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO330_000647"
     CDS             complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO330_000647"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5881634.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3587..3631
                     /locus_tag="OO330_000648"
     CDS             3587..3631
                     /locus_tag="OO330_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881635.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO330_000649"
     CDS             complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO330_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5881636.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5390..5476
                     /gene="leuL"
                     /locus_tag="OO330_000650"
     CDS             5390..5476
                     /gene="leuL"
                     /locus_tag="OO330_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5881637.1"
                     /translation="MTHIIRFIGLLLLNASSLRGRRVSGIQH"
     gene            5569..7140
                     /gene="leuA"
                     /locus_tag="OO330_000651"
     CDS             5569..7140
                     /gene="leuA"
                     /locus_tag="OO330_000651"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5881638.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7140..8231
                     /gene="leuB"
                     /locus_tag="OO330_000652"
     CDS             7140..8231
                     /gene="leuB"
                     /locus_tag="OO330_000652"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5881639.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8234..9634
                     /gene="leuC"
                     /locus_tag="OO330_000653"
     CDS             8234..9634
                     /gene="leuC"
                     /locus_tag="OO330_000653"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5881640.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9645..10250
                     /gene="leuD"
                     /locus_tag="OO330_000654"
     CDS             9645..10250
                     /gene="leuD"
                     /locus_tag="OO330_000654"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5881641.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10299..11273)
                     /locus_tag="OO330_000655"
     CDS             complement(10299..11273)
                     /locus_tag="OO330_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5881642.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALARVELPSENALSMQGDWQDSNVRMLFVASTLMW
                     TCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVI
                     AVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLF
                     TNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV"
     gene            complement(11375..11506)
                     /gene="sgrT"
                     /locus_tag="OO330_000656"
     CDS             complement(11375..11506)
                     /gene="sgrT"
                     /locus_tag="OO330_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5881643.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11595..13250
                     /gene="sgrR"
                     /locus_tag="OO330_000657"
     CDS             11595..13250
                     /gene="sgrR"
                     /locus_tag="OO330_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5881644.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13284..13383
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13414..14397
                     /gene="thiB"
                     /locus_tag="OO330_000658"
     CDS             13414..14397
                     /gene="thiB"
                     /locus_tag="OO330_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5881645.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14373..15983
                     /gene="thiP"
                     /locus_tag="OO330_000659"
     CDS             14373..15983
                     /gene="thiP"
                     /locus_tag="OO330_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5881646.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGIRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            15967..16665
                     /gene="thiQ"
                     /locus_tag="OO330_000660"
     CDS             15967..16665
                     /gene="thiQ"
                     /locus_tag="OO330_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5881647.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16779..17543)
                     /locus_tag="OO330_000661"
     CDS             complement(16779..17543)
                     /locus_tag="OO330_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5881648.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17629..18507)
                     /gene="araC"
                     /locus_tag="OO330_000662"
     CDS             complement(17629..18507)
                     /gene="araC"
                     /locus_tag="OO330_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5881649.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            18846..20546
                     /gene="araB"
                     /locus_tag="OO330_000663"
     CDS             18846..20546
                     /gene="araB"
                     /locus_tag="OO330_000663"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5881650.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSMVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20557..22059
                     /gene="araA"
                     /locus_tag="OO330_000664"
     CDS             20557..22059
                     /gene="araA"
                     /locus_tag="OO330_000664"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5881651.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22259..22954
                     /gene="araD"
                     /locus_tag="OO330_000665"
     CDS             22259..22954
                     /gene="araD"
                     /locus_tag="OO330_000665"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5881652.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23029..25380
                     /gene="polB"
                     /locus_tag="OO330_000666"
     CDS             23029..25380
                     /gene="polB"
                     /locus_tag="OO330_000666"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5881653.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25545..28451
                     /gene="rapA"
                     /locus_tag="OO330_000667"
     CDS             25545..28451
                     /gene="rapA"
                     /locus_tag="OO330_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5881654.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28463..29122
                     /gene="rluA"
                     /locus_tag="OO330_000668"
     CDS             28463..29122
                     /gene="rluA"
                     /locus_tag="OO330_000668"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5881655.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29239..30054)
                     /gene="djlA"
                     /locus_tag="OO330_000669"
     CDS             complement(29239..30054)
                     /gene="djlA"
                     /locus_tag="OO330_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5881656.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30309..32663
                     /gene="lptD"
                     /locus_tag="OO330_000670"
     CDS             30309..32663
                     /gene="lptD"
                     /locus_tag="OO330_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5881657.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32716..34002
                     /gene="surA"
                     /locus_tag="OO330_000671"
     CDS             32716..34002
                     /gene="surA"
                     /locus_tag="OO330_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5881658.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34002..34991
                     /gene="pdxA"
                     /locus_tag="OO330_000672"
     CDS             34002..34991
                     /gene="pdxA"
                     /locus_tag="OO330_000672"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5881659.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            34988..35809
                     /gene="rsmA"
                     /locus_tag="OO330_000673"
     CDS             34988..35809
                     /gene="rsmA"
                     /locus_tag="OO330_000673"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5881660.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            35812..36189
                     /gene="apaG"
                     /locus_tag="OO330_000674"
     CDS             35812..36189
                     /gene="apaG"
                     /locus_tag="OO330_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5881661.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36196..37038
                     /gene="apaH"
                     /locus_tag="OO330_000675"
     CDS             36196..37038
                     /gene="apaH"
                     /locus_tag="OO330_000675"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5881662.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37116..37595)
                     /gene="folA"
                     /locus_tag="OO330_000676"
     CDS             complement(37116..37595)
                     /gene="folA"
                     /locus_tag="OO330_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5881663.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37786..39648)
                     /gene="kefC"
                     /locus_tag="OO330_000677"
     CDS             complement(37786..39648)
                     /gene="kefC"
                     /locus_tag="OO330_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5881664.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39641..40171)
                     /gene="kefF"
                     /locus_tag="OO330_000678"
     CDS             complement(39641..40171)
                     /gene="kefF"
                     /locus_tag="OO330_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5881665.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40219..41613)
                     /locus_tag="OO330_000679"
     CDS             complement(40219..41613)
                     /locus_tag="OO330_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5881666.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41672..41959)
                     /gene="fixX"
                     /locus_tag="OO330_000680"
     CDS             complement(41672..41959)
                     /gene="fixX"
                     /locus_tag="OO330_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5881667.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(41956..43242)
                     /locus_tag="OO330_000681"
     CDS             complement(41956..43242)
                     /locus_tag="OO330_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5881668.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43293..44234)
                     /locus_tag="OO330_000682"
                     /pseudo
     CDS             complement(43293..44234)
                     /locus_tag="OO330_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44249..45019)
                     /locus_tag="OO330_000683"
     CDS             complement(44249..45019)
                     /locus_tag="OO330_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5881669.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTFTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45491..47005
                     /gene="caiT"
                     /locus_tag="OO330_000684"
     CDS             45491..47005
                     /gene="caiT"
                     /locus_tag="OO330_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5881670.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47036..48178
                     /gene="caiA"
                     /locus_tag="OO330_000685"
     CDS             47036..48178
                     /gene="caiA"
                     /locus_tag="OO330_000685"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5881671.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48365..49414
                     /gene="caiC"
                     /locus_tag="OO330_000686"
                     /pseudo
     CDS             48365..49414
                     /gene="caiC"
                     /locus_tag="OO330_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
     gene            49523..50308
                     /gene="caiD"
                     /locus_tag="OO330_000687"
     CDS             49523..50308
                     /gene="caiD"
                     /locus_tag="OO330_000687"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5881672.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSTGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            50314..50904
                     /gene="caiE"
                     /locus_tag="OO330_000688"
     CDS             50314..50904
                     /gene="caiE"
                     /locus_tag="OO330_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5881673.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(50990..51385)
                     /gene="caiF"
                     /locus_tag="OO330_000689"
     CDS             complement(50990..51385)
                     /gene="caiF"
                     /locus_tag="OO330_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5881674.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(51647..54868)
                     /gene="carB"
                     /locus_tag="OO330_000690"
     CDS             complement(51647..54868)
                     /gene="carB"
                     /locus_tag="OO330_000690"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5881675.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLALSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(54886..56034)
                     /gene="carA"
                     /locus_tag="OO330_000691"
     CDS             complement(54886..56034)
                     /gene="carA"
                     /locus_tag="OO330_000691"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5881676.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(56490..57311)
                     /gene="dapB"
                     /locus_tag="OO330_000692"
     CDS             complement(56490..57311)
                     /gene="dapB"
                     /locus_tag="OO330_000692"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5881677.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(57478..58392)
                     /gene="rihC"
                     /locus_tag="OO330_000693"
     CDS             complement(57478..58392)
                     /gene="rihC"
                     /locus_tag="OO330_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5881678.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(58458..59408)
                     /gene="ispH"
                     /locus_tag="OO330_000694"
                     /gene_synonym="lytB"
     CDS             complement(58458..59408)
                     /gene="ispH"
                     /locus_tag="OO330_000694"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5881679.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(59410..59859)
                     /gene="fkpB"
                     /locus_tag="OO330_000695"
     CDS             complement(59410..59859)
                     /gene="fkpB"
                     /locus_tag="OO330_000695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5881680.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(59946..60440)
                     /gene="lspA"
                     /locus_tag="OO330_000696"
     CDS             complement(59946..60440)
                     /gene="lspA"
                     /locus_tag="OO330_000696"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5881681.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(60440..63256)
                     /gene="ileS"
                     /locus_tag="OO330_000697"
     CDS             complement(60440..63256)
                     /gene="ileS"
                     /locus_tag="OO330_000697"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5881682.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(63299..64240)
                     /gene="ribF"
                     /locus_tag="OO330_000698"
     CDS             complement(63299..64240)
                     /gene="ribF"
                     /locus_tag="OO330_000698"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5881683.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(64248..64466)
                     /locus_tag="OO330_000699"
     CDS             complement(64248..64466)
                     /locus_tag="OO330_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5881684.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            64569..64832
                     /gene="rpsT"
                     /locus_tag="OO330_000700"
     CDS             64569..64832
                     /gene="rpsT"
                     /locus_tag="OO330_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5881685.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        15772 a        17750 c        16778 g        14861 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaacca tagccattgt actggtatgg gttaaattca ttaacatcta
     1141 agcgcagaaa attaactgcg gcaactgctt ttattccagg ttcccactga tttcgcaaaa
     1201 aaagcccaac gtgacatgtt gggctgcaaa ttgcgcactg agatcattct caacgcatta
     1261 ttttatcgcc gcgcgaaagt ccgaccacac cagagcgagc aacttccaca attttcgcca
     1321 catcgcgaat cgatgctaaa aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg
     1381 tataaagcga gggggtgaca tcgataattt gcccacggaa tatttccgta ttacgtttca
     1441 cttcgtcacg cccgtaaccg ctggcctgaa ttttcaccag catgatttcc cgctcaacat
     1501 gcgcgccctg ccccaactca ctcacgcgca agacatcgac cagcttgtgt aattgctttt
     1561 cgatctgctc aagtactttt tcatcgccca cggtctggat ggtcatacgc gataatgtcg
     1621 gatcgtcggt tggcgcaacg gtcaggcttt caatgttgta gcctcgctgg gaaaaaaggc
     1681 caatcacgcg ggataacgcg cctgattcat tttcgagtaa gactgataat atccggcgca
     1741 taatcaggtt ctctccgttt tgcttaacca catttcatcc attccgcccc cgcgaatctg
     1801 catcgggtag acgtgctcac tgccatcgac ggtaacatca acaaacacca ggcgattatt
     1861 gcgcacctgt tccagcgcct cgctaagttt gctttccagc tcatgcggat gagagatctg
     1921 gatcccgacg tgaccatagg cttccgccag acggacgaaa tcgggtagtg attgcatata
     1981 agattgtgaa tgacggccgg aatagatcat gtcctgccac tgtttcacca tccccagata
     2041 gcggttattg agattcacca ccagtacggg caactcgtat tgcaacgcgg tagacagttc
     2101 ctggatgttc atctgaatac tgccgtcgcc agtgacgcaa accacggttt cttctggcaa
     2161 cgccattttg acgcccagtg ccgcaggtaa accaaaaccc atcgtgccga gaccgccgga
     2221 attgatccag cgacgcggtt tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg
     2281 cccgacatcg gacgtcacgt aagcgtcccc attagtcaac cgccaaagag tctcgatcac
     2341 cgcctgcggt ttaatctttt cactgtgagt gtcatatttc aggcactgaa gagcgcgcca
     2401 ctgttcaatc tgctgccacc agtcgcggat ctcatccagc ggttgatggg cggattcttg
     2461 cgacaggagt tcaagcattt gttcgaggac ctggcgagca tcccccacaa tcgggatatc
     2521 cgcagtcacg gttttagaaa tggaagtagg atcaatatcg atatgcagaa cagtggcatt
     2581 tgggcagtac tttgccagat tgttcgtcgt tcggtcatca aatcgtaccc cgacggcgaa
     2641 aatcacatcc gcgttatgca tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat
     2701 gcccagtgcc tgacgatgcg ttgccggaaa cgcccccagc cccatcaatg aggaaacaac
     2761 gggcagattc aacgcctcca ccgtttcttt caactgctga tggcagcccg ccatgattgc
     2821 cccaccgcct acgtagacaa ccggtttttt tgccgctacc agcgtttgca gagcacgctt
     2881 aatttgccct ttatgtccgg tagtagtggg attgtaagaa cgcatactga ccgactcagg
     2941 gcagacatag ggtaatttgt tcgccggatt aagaatatct ttcggtaaat caacgaccac
     3001 tggtcctggg cgaccacttg ccgccagcca gaaagccttt ttcagcacct gcggaatgtc
     3061 ttccgtttgc ttaaccagaa aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc
     3121 gcactcctga aaggcatcgt aacctatcaa cgaggtcgct acctgcccgg aaaggacaac
     3181 taatggaatg gaatccatat aagcggtggc gatgccggta atcgcattgg tcgcccctgg
     3241 acccgacgtt accagcacga cgccgacttc cccggtcgca cgcgccaggc catcggccat
     3301 atgcaccgcc gcctgctcat gacgaactaa tacatgatca ataccaccca cggtatgcaa
     3361 tgcatcgtaa atatcaagga ctgcgcctcc gggataaccg aatacttgtt taacgccctg
     3421 atcgataagc gatcggacga ccatctcggc tccagacaac atctccatgg cctgcctcac
     3481 tgtttgacgg aaaaaatgtg taaaacacat taaccgtccg atcttgctta agcaattggc
     3541 agccagagac ataaacgcag ggtaataaga ataaacagga ggaaaggtga aaagaaacga
     3601 taaaatcctc cattacgaag gattaaattg ataataaatc ggctgaatcc cacaacttac
     3661 agcaatttct ctattttttt aattaatcct aaaatagaga gtccacacat tcagaataaa
     3721 attctacatt caataaaaaa tgcagaataa accagacatt catgtctggc ctattctgca
     3781 ttcagttagc gtttgcaaat tgagactaat tgttcttcca tccactgatg gcctttatcg
     3841 cgcccggcag cttcatgcca agagagataa caggttctgc tgttttgttt taacggcagc
     3901 ggtaatacct gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata
     3961 gcgaccaaat gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac
     4021 gcgatactgg cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga
     4081 tcgagcgaaa ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccttta
     4141 attgttggat gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg
     4201 gtaaattcag gacgatggaa gtcttcataa ctaatcacaa actccgtttc ctgataacgc
     4261 agctgatgtt cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca
     4321 atctgctcaa tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca
     4381 caaagatgaa atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt
     4441 tgtactagtt gcaatgcctg gcgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt
     4501 tgaataccac ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca
     4561 acagcgttac tgaccgcagg ttgcgacata cccagaacat gagcggcacg ggtaatattt
     4621 tgctcctgca tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt
     4681 ggcttgctta actccgccgt ctctgaatga tctgtttgta cctctggcat acttaactcc
     4741 actgtcacac ttaactccct ttcccttatt ggaatgcaga gaaataatcc tgaagaatat
     4801 gatttatcat gcatataaaa taagaaaaag caaaatgagt aaaattcgga aaacataaag
     4861 atacgggttc tgataaaaac agaatccatt gccgtggaat gagtcattta ccacagcata
     4921 ataatccata atgtttttgt tgcgaaaaca atctaatcat aactaccgcg aatactcaat
     4981 catctacaaa atggattaaa tgtgcttatt taataattaa tttatatatt taatgcatta
     5041 attcttaaca ttaattgatc aataatattc accaaatcaa tatcaaaaaa aatcgcaaaa
     5101 catataattc aatacaaatc atcaggatag gttttgcaac gcgtgcattt tgtccccttt
     5161 ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg
     5221 gtagttaagc agaaattaat atcgcttact ttaaccaccg tagcacaatt agctaatttt
     5281 acggatgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga catccgtttt
     5341 tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa tgactcacat
     5401 cattcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta gacgagtgag
     5461 cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt
     5521 tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat gagccagcaa
     5581 gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc aagcttgagt
     5641 gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa
     5701 gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag
     5761 gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg
     5821 gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc tacctcccca
     5881 atgcacatcg ccaccaagct gcgcagcacc ctagacgagg tgatcgaacg cgctatctat
     5941 atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga agatgccggg
     6001 cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc
     6061 accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc cggaatcatc
     6121 agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt acatacccac
     6181 gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg tgcacgccag
     6241 gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct ggaagaagtc
     6301 atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat taatcaccaa
     6361 gagatatggc gcaccagcca gttagttagc cagatttgta atatgccgat cccggcaaac
     6421 aaagccattg ttggcagcgg cgcattcgct cactcctccg gtatccacca ggatggcgtg
     6481 ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct gaaccaaatc
     6541 cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat ggatgagatg
     6601 gggtataaag aaagtgaata taatttagac aatttgtacg atgctttcct gaagctggcg
     6661 gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat cggtaagcag
     6721 caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg ctctaacgat
     6781 atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga agccgccaac
     6841 ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga ctataacgtc
     6901 gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg
     6961 gatattgtca ccaactacaa cggtcgccgc ttccacggcg tcggcctggc caccgatatt
     7021 gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg tgccgcagaa
     7081 gtcgaaaaag agttgcaacg caaaactcaa cacaacgaaa acaacaagga aaccgtgtga
     7141 tgtcgaagaa ttaccatatt gccgtattgc cgggagacgg tattggtccg gaagtgatga
     7201 cccaggcgct gaaagtgctg aatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca
     7261 gccattacga tgtaggcggc gcagccattg ataaccacgg gcaaccactg ccgcctgcaa
     7321 cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc ggcccgaagt
     7381 gggaacattt accaccagac cagcaaccag aacgcggcgc gctgctgccg ctgcgtaagc
     7441 acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg gaagcattct
     7501 gtccgctgcg tgcagacatt gccgctaacg gcttcgacat ccagtgcgtg cgcgaactga
     7561 ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag
     7621 cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc atcgcgtttg
     7681 aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac gtgctgcaat
     7741 cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg gatgtcgaac
     7801 tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca tcacagtttg
     7861 acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc gcaatgatca
     7921 ctggctcgat gggcatgttg ccttccgcca gcctgaacga gcaaggtttt ggactgtatg
     7981 aaccggcggg cggctcggca ccagatatcg caggcaaaaa catcgccaac ccgattgcac
     8041 aaatcctgtc gctggcactg ctgctgcgtt acagcctgga tgccgatgat gcggcttccg
     8101 ccattgaacg cgccattaac cgtgcattag aagaaggcat tcgcaccggg gatttagccc
     8161 gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc tatgtggcag
     8221 aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc acgtagtgta
     8281 cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc atgaagtgac
     8341 ctcaccgcag gcgttcgatg gcctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa
     8401 aaccttcgcc actatggatc acaacgtctc tacccagacc aaagacatta atgcctgtgg
     8461 tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga
     8521 gctgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc cggaacaggg
     8581 cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca cccacggcgc
     8641 gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac tggcaacaca
     8701 aaccctgaaa cagggccgtg cgaagaccat gaaaattgaa gtccagggca aagccgcgcc
     8761 gggcattacc gcaaaagata tcgtgctggc aattatcggt aaaactggca gcgcaggcgg
     8821 caccgggcat gtggtggagt tttgcggcga agcaattcgt gatttaagca tggaaggtcg
     8881 tatgaccctg tgcaatatgg cgatcgaaat gggcgcaaaa gccggtctgg ttgctcctga
     8941 cgaaaccacc tttaactatg tcaaaggccg tctgcatgcg ccgaaaggca aagatttcga
     9001 cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt tcgataccgt
     9061 tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca atcccggcca
     9121 ggtgatttct gtgaacgaca atattcccga tccggcttcg tttgccgatc cggttgaacg
     9181 tgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggtattc cgctgaccga
     9241 agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tcgcgcattg aagatttacg
     9301 cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt
     9361 tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata aaatatttat
     9421 tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga
     9481 ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg aaggccgcca
     9541 ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg ctgctgtgac
     9601 cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca gagaaattta
     9661 tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc gatgcaatca
     9721 tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg
     9781 actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc
     9841 cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt ggctcctcgc
     9901 gtgagcacgc gccctgggca ttgaccgact acggttttaa agtggtgatt gcgccgagtt
     9961 ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg aaattaagcg
    10021 atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc catttcgacg
    10081 tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg
    10141 ccttccgccg ccactgcatg atgaatggtc tggacagcat cggcctgacc ctgcagcacg
    10201 acgacgctat tgcgtcttat gaagcaaaac aacctgcgtt tatgaattag accccttgcc
    10261 cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg
    10321 gttaaaaata atgcgacaac agaaataatc gcaattaccc agtagacagc aaagtgcccc
    10381 caactttgtg caattgctcc ctgaataaca ccagccagaa ttaccccggt agagatagtg
    10441 ttggtaaata aggtggtagc tgcccccgct cttccaggca ttaaatcctg aaaccatagc
    10501 atcccaatac ccgcaacaat gccgataaat acagcgttaa aaagttgcag cgtcatcaac
    10561 gccatacggc tatgaaagaa aatcaatcca gtgtaaaaca gcactcctgc caccactgca
    10621 atgaccatca ttcgccgctt accataacgt ttgacatagt agccagccag aatcattgct
    10681 ggtatttcca gtccagctgc cgtccccatc aggaaacccg ccagtttgtc tggcaatcct
    10741 aaatcgctac tgatccacaa tggcatatca ataatgtaca tagtgttgca ggtccacatt
    10801 aacgtcgagg cgacaaataa catccgtacg ttactatcct gccagtcacc ttgcattgat
    10861 aaagcatttt ccgacggcag ttctacccgc gccagagcaa ataactgtgg cattgccgta
    10921 ttggccagag atgccagaag cacaccacag gtgataagcg tcagataatg acgattaaat
    10981 gcaaacagta gcgcattgcc aatagccatc aaacagcaaa atataatcag ttttcgccga
    11041 tcgccctgac tgtcagaacg ttttgccaac cagaggctta cgccgatccc agcaatagca
    11101 ttcaccgtat aaaagagtcc gatccagaaa ggttgcgcgc caacctcacg actcagaaat
    11161 aagctcaatg taggagcctg tagcgccccg gccaccccca tcataaaagc gaccagcata
    11221 aatgccgcgt aaacaccgtt catacggcga gccatcgtca ttatccagat catacgttcc
    11281 ctttttagcg cggcgagaat aaaaaaaacc agcaggtata atctgctggc gggtgatttt
    11341 acaccaatac tcagtcacac atgatgcagg caagtcaact ttcagaattg cggtcatccc
    11401 actgcatcag tccttcaagc atggttaatc gttgtgggac gcttaaccaa cgcaaccagc
    11461 acaacttcgc tgtcgcggta aaatagtgct gataaaactg acgcatgggg cacctccttg
    11521 cttcatcgtt aggaattatt gacttaatat agggaaaata aaattgctgt cttttgcgca
    11581 ggagttcccc ttttatgcca tctgctcgtc tgcaacaaca gttcatccgc ctgtggcaat
    11641 gctgcgaggg taaatcgcag gacacaacgc tcaacgaact ggcagcgtta ttgagctgct
    11701 cgcgtcgtca tatgcgtacc ctgctcaaca ccatgcagga tcgcggctgg ctgacgtggg
    11761 aagcggaagt cgggcgcggt aaacgctcgc gtctgacatt cctctatacc gggctggcgc
    11821 ttcagcaaca gcgggcggaa gacctgctgg agcaggatcg tatcgaccaa ctagtgcagt
    11881 tggttggcga caaagcgact gtgcggcaaa tgctggtttc tcatctgggc cgcagcttcc
    11941 gccaggggcg gcacatcctg cgcgtgctct actatcgtcc gttgcgtaat ctgctacctg
    12001 gcagcgcatt gcgccgttcc gaaacccata ttgcccggca aatcttcagt tcgctaacgc
    12061 gcataaatga ggaaaatggg gaactggaag cagacatcgc ccaccactgg cagcaaatat
    12121 caccgcttca ctggcgtttc tttttgcgtc caggagtcca ttttcatcat ggtcgtgaac
    12181 tggaaatgga cgacgtgatc gcctctttaa aacgaatcaa tacgctgccg ctctattcgc
    12241 atattacgga cattgtgtcg ccgacgccct ggacgctgga tatccatctc acgcagccgg
    12301 accgctggtt gccgttactg ctggggcaag ttccggcgat gatcctgccg tgcgaatggg
    12361 aaaccctcag taactttgcc agccatccta ttggcaccgg tccgtatgcg gtgattcgca
    12421 acagcaccaa tcaactgaaa attcaggcat tcgatgactt cttcggttac cgggcattaa
    12481 tcgacgaagt taacgtctgg gttctgccgg aaattgccga cgagccagcc ggtggtctga
    12541 tgctaaaagg tccacagggc gagaaaaaag agattgaaag ccgcctggag gaaggttgct
    12601 actatttact gttcgacagc cgcacccatc gcggggcgaa tcagcaagtc agggactggg
    12661 taagctatgt gctttctcca actaatctgg tctatttcgc tgaggaacag taccagcaac
    12721 tgtggttccc ggcttatgga ctgctccccc gttggcacca tgcccgcacc ataaagagcg
    12781 aaaaaccggc tggcctggaa agcctcaccc tgacctttta tcaggatcac agtgagcatc
    12841 gggtgattgc cgggatcatg cagcagattc tggcaagtca ccaggtcacg ctggaaatca
    12901 aagagatcag ctacgatcag tggcatgaag gagagatcga gagcgatatc tggcttaata
    12961 gcgccaactt tacgctgccg ctggattttt cgctgttcgc gcacttgtgc gaggtgccgc
    13021 tgctccaaca ctgtattccg atcgactggc aagccgacgc cgcccgctgg cgcaatggcg
    13081 aaatgaacct ggcgaactgg tgccagcaac tggtcgccag taaagcaatg gtgccgctta
    13141 tccaccactg gctgatcatt cagggacaac gcagtatgcg cggcctgcgt atgaataccc
    13201 tcggctggtt cgattttaaa tcagcgtggt ttgcgccacc ggatccatga ttgctggtag
    13261 ataacaaaat cactacacta acgccgttct caacggggtg ccacgcaaac gcgtgcgctg
    13321 agaaaatacc cgtcgaacct gatccggata acgccggcga agggatttga ggctccttct
    13381 caagtccttt gccactcttt tttgaggtgc aaagtgttaa aaaaatgtct tcccctgctg
    13441 ttgctgtgca cagcgcccgt tttcgctaaa cccgttctga ctgtttatac ctacgattcc
    13501 ttcgccgccg actgggggcc tggtccaaag atcaaaaaag cttttgaagc cgactgtaac
    13561 tgcgaactga aactggtggc gctggaagat ggcgtttcgc ttctcaaccg tctacggatg
    13621 gaaggcaaaa atagtaaagc cgatgtggtg ctggggctgg ataacaatct gttagacgcc
    13681 gccagtaaaa ccggactatt tgccaaaagc ggtgtggcag cggatgccgt taacgttccc
    13741 ggcggctgga ataatgacac tttcgtcccg tttgattacg gctatttcgc cttcgtctat
    13801 gacaagaaca aactgaaaaa cccgccgcaa agcctgaaag aattggttga gagcgatcag
    13861 aactggcggg tgatttatca ggatccgcgt accagcacgc cggggctggg tctgctgttg
    13921 tggatgcaaa aagtctatgg cgatgacgcc ccacaagcct ggcaaaaact ggcgaagaaa
    13981 accgtcacgg tcaccaaagg ctggagcgaa gcctacggcc tgtttttaaa aggtgaaagc
    14041 gatctggtac tgagttacac cacctctccg gcttatcaca ttctcgaaga gaaaaaagat
    14101 aactacgccg ccgcgaactt tagcgaaggt cactatctgc aggtggaagt cgccgcccgc
    14161 accgctgcca gcaagcagcc ggagctggcg caaaaattcc tccagtttat ggtttctccg
    14221 gcttttcaga atgcgatccc aaccggtaac tggatgtatc cggtggcaaa tgtcacgctg
    14281 cctgccgggt ttgaacaatt gaccaaaccg gccaccacgc tggagttcac accagctgaa
    14341 gtggcggcac aacgtcaggc atggattagc gaatggcaac gcgccgtcag ccgttaattc
    14401 ccggctggtt aattccaggt gtaagcgccg ccacgctggt ggtagcggtt gcgctcgcgg
    14461 cgtttctcgc cctgtggtgg aacgcgccgc agggtaactg ggtggcagtc tggcaggaca
    14521 gctacctgtg gcatgtggtg cgcttctcct tctggcaggc gtttctctcg gcgctactct
    14581 ctgtcgtacc cgcgatattc ctcgcccgcg ccctctatcg caggcgcttt ccgggtcggc
    14641 tggcgctgtt gcgtctgtgc gcaatgacct tgatcctccc ggtgctagtt gctgttttcg
    14701 gcattcttag cgtctatggt cgtcagggct ggctggcatc gctctgccaa tcgctcggtc
    14761 tggagtggac cttttcgccc tacggcctgc aaggcatttt gctggcgcac gtatttttta
    14821 atctgccgat ggcgagccgc ttattactcc aggcactgga aaacattccc ggcgaacaac
    14881 gtcagcttgc cgcccagctt gggatacgcg gctggcattt tttccgcttc gtcgaatggc
    14941 cgtggctacg gcgacaaatc ccgccggttg ccgcgcttat ctttatgctc tgtttcgcca
    15001 gcttcgccac cgtgctatcg ctgggcggcg gtccgcaggc aaccactatc gagctggcta
    15061 tttatcaggc gctgagttac gactacgatc ctgcccgcgc ggcgatgctg gcgctgctcc
    15121 agatggtgtg ttgccttggg ctggtgctgt tgagtcagcg actaagtaag gccattgcgc
    15181 ccggcaccac gctgctgcaa ggctggcgcg atccggacga tcgtctgcat agccgcattt
    15241 gcgacacggt gttaatagtg ctggcgctgc tgctgttgct gccaccgttg ctggcggtga
    15301 tcgtcgatgg ggtaaatcgc cagttgccgg aagtgctggc acaaccggtg ctgtggcagg
    15361 cgctgtggac ctcgttgcgt attgcgctgg cggcaggtgt attgtgcgta gtgctgacca
    15421 tgatgctgct atggagcagt cgcgaactgc gggcgcggca gaaaatgctg gcgggtcagg
    15481 cgctggagat gagcggcatg ttgatcctcg ccatgccggg gattgtgctg gctaccggct
    15541 tctttttact gctcaacaac actatcggcc tgccacaatc tgctgacggc attgtgattt
    15601 tcaccaatgc gttaatggcg atcccttatg cgctgaaagt actggaaaac ccgatgcgcg
    15661 atatcaccgc ccgctacagt atgttgtgtc agtcgctggg cattgaaggc tggtcgcgct
    15721 taaaagtggt cgagctgcgc gccctgaaac gtccactggc gcaggcgctg gcttttgcct
    15781 gcgtgctgtc gattggtgat tttggcgtag tggcgttgtt cggtaacgat gatttccgta
    15841 ccctgccgtt ttatctctac cagcaaattg gctcctatcg cagccaggac ggcgcggtca
    15901 ccgcgttaat tctgctacta ctctgttttc tgctgtttac cgtgattgaa aaactaccgg
    15961 ggcgaaatgt taaaactgac tgatatcacc tggctttatc accatttgcc gatgcgtttt
    16021 agcttaacgg tagaacgcgg cgagcaggtg gcaatcctcg ggccaagcgg cgcgggtaaa
    16081 agtaccctgc tgaatttgat tgccggtttt ctgacgccag ccagcggttc gctgactatc
    16141 gatggtgttg atcacaccac gacgccgccg tcacgccgtc cggtgtcgat gctgtttcag
    16201 gagaacaacc tgttcagcca tctgacggtc gcgcagaaca tcgggctggg gctaaatccg
    16261 gggttgaaac tgaacgcggc acagcaggag aaaatgcacg cgattgcccg tcagatgggg
    16321 attgataatt taatggcgcg gttaccgggc gagctttccg gcggtcagcg acagcgcgtg
    16381 gcgttagcgc gttgtctggt gcgcgaacag ccgattttat tgctcgatga accgttctct
    16441 gcgctcgatc cggcgttacg tcaggagatg ctgacgctgg tgagcacgag ctgccagcag
    16501 caaaaaatga cgctattgat ggtgtcgcac agtgtggaag atgcggcgcg gatcgccaca
    16561 cgctcggtgg tggtcgccga cgggcgcatc gcctggcagg gtaagactga agaattgttg
    16621 agcggtaagg cgagtgcttc ggcaatattg gggattacgg gttagtatgc actgtcagat
    16681 gcggcgtgaa caccttatcc gacctacgga cagtgcgttt tgtaggcctg ataagacgcg
    16741 gtcagcgtcg catgaggcaa cccgcacaag acaatcccct aaaccccaac cactttacgc
    16801 aaaatatcga tatacaccgg catcaacggg tggcgaatta acaccaccag cgccaccacg
    16861 ccgatggcag aaatcaacgg cgtcagccac aacaaacggc cgcgcgacaa ataatgactt
    16921 aaacgatcgg ttgctttacc gctgcgccat aaccgccagc acagccagcc accaacccac
    16981 aaaaataccg ccgtcgccag cagcaaccat ttaaactcgc cgctctgcat tccggctgga
    17041 atatcgatcg ccgcgcccgc cagaatccca ggcaggaagt aaaacggtgg ccacagcagg
    17101 cagccgataa tattcggcgt aatgaatttt gccaccggca gatccagcat tcccgccacc
    17161 attggcacca gcggacgcgt cgggccaaca aagcgaccga ccagaatggt gaacatgctg
    17221 tgttgatgca acgcatgttc agttttatcg agtaatgctt tgtttttctt cagaaatgac
    17281 cagcgatgca acggcttttt aaaacgccag cccagccaga aggagatcca gtcgcccagc
    17341 agacaaccga caatccctgc cagccaagcg tgccagaaac ttaactcgcc gctgccaatc
    17401 agcgctccca gccccgccat cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc
    17461 gactccagaa aggccaccaa caccaccgcc atcaatgaat acacggtaga ttgggtaata
    17521 aagtgttcca gcaatgcttg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca
    17581 aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt
    17641 gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc
    17701 ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc
    17761 gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg
    17821 gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg
    17881 cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag
    17941 gtgatcgctg atgtactgac aagcctcgcg tacacgatta tccatcggtg gatggagcga
    18001 ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag
    18061 ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa
    18121 atacggctgg tgcgcttcat ccgggcgaaa gaaacccgta ttggcaaata ttgacggcca
    18181 gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc
    18241 gcgagcctcc ggatgacaac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc
    18301 acccggtcgg cagacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt
    18361 gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc
    18421 gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag
    18481 caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca gagaagaaac
    18541 caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc tcttctcgct
    18601 aacccaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcagga ccaaagccat
    18661 gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgattattt
    18721 gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag cggatcctac
    18781 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt ggatggagtg
    18841 aaacgatggc gattgcaatt ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg
    18901 tggactgcgc cagcggtgaa gagatcgcca ccagcgtaga gtggtatccc cgttggcaaa
    18961 aagggcaatt ttgtgatgcc ccgaataacc agttccgtca tcatccgcgt gactacattg
    19021 agtcaatgga agcggcactg aaaaccgtgc ttgcagagct tagcgtcgaa cagcgcgcag
    19081 ctgtggtcgg gattggcgtt gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg
    19141 gtaacgtgct ggcgctgcgc ccggagtttg ccgaaaaccc gaacgcgatg ttcgtattgt
    19201 ggaaagacca cactgcggtt gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg
    19261 gcaacgttga ctactcccgc tatattggcg gtatttattc cagcgaatgg ttctgggcaa
    19321 aaatcctgca tgtgactcgc caagacagcg ccgtggcgca atctgccgca tcgtggattg
    19381 agctgtgcga ctgggtgcca gctctgcttt ccggtaccac ccgcccgcag gatattcgtc
    19441 gcggacgttg cagcgccggg cataaatctc tgtggcacga aagctggggc ggcttgccgc
    19501 cagccagttt ctttgatgag ctggacccga tcctcaatcg ccatttgcct tccccgctgt
    19561 tcactgacac ctggactgcc gatattccgg tgggcacctt atgcccggaa tgggcgcagc
    19621 gtctcggcct gcctgaaagc gtggtgattt ccggcggcgc gtttgactgc catatgggcg
    19681 cagttggcgc aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg
    19741 acattctgat tgccgacaaa cagagcgttg gcgagcgggc agttaaaggt atttgcggtc
    19801 aggttgatgg cagcatggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg
    19861 gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc gctggaacag cttgccgccc
    19921 agcatccgga actgaaagcg caaatcaacg ccagccagaa acaactgctt ccggcgctga
    19981 ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta
    20041 acggtcgtcg cacgccaaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc
    20101 tcgctaccga cactccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac
    20161 gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt caataacgtg atggcgctgg
    20221 gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc
    20281 cgctgcaaat tgttgcctct gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg
    20341 tcgccgcgaa agtgcacgca gacatcccat cagcccagca aaaaatggcc agtgcggtag
    20401 agaaaaccct gcaaccgcgc agcgaacagg cacaacgctt tgaacagctt tatcgccgct
    20461 atcagcaatg ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg
    20521 ctgcccaggc cgttgcgact ctataaggac acgataatgg cgatttttga taattatgaa
    20581 gtgtggtttg tcattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc
    20641 caacatgccg agcacgttgt taatgcgctg aatacggaag cgaaactgcc ctacaaactg
    20701 gtgttgaaac cgctgggcac cacgccggat gaaatcaccg ctatttgccg cgacgcgaat
    20761 tacgacgatc gttgcgctgg tctggtggtg tggctgcaca ccttctcccc ggccaaaatg
    20821 tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac
    20881 gcggcgctgc cgtgggacag tatcgatatg gactttatga acctgaacca gactgcacat
    20941 ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtcgttacc
    21001 ggtcactggc aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggtc
    21061 tctaaacagg atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg
    21121 gcggtcaccg atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc
    21181 tgggcggttg gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg
    21241 ctggtcgatg agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcgaa
    21301 aaacgacaga acgtgctgga agcggcgcgt attgagctgg ggatgaagcg tttcctggaa
    21361 caaggtggct tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt
    21421 ccaggtctgc ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac
    21481 tggaaaaccg ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc
    21541 acctccttta tggaggacta cacctatcac ttcgagaaag gtaatgacct ggtgctcggc
    21601 tcccatatgc tggaagtctg cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt
    21661 cagcatctcg gtattggtgg taaggacgat cctgcccgac tgatcttcaa tacccaaacc
    21721 ggtccagcga ttgtcgccag cctgattgat ctcggcgatc gttaccgtct gctggttaac
    21781 tgtatcgaca cggtgaaaac accgcactcc ctgccgaaac tgccggtggc gaatgcactg
    21841 tggaaagcgc aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg
    21901 caccataccg tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg
    21961 cacgacattg aaatcacggt gattgataac gacacccgcc tgccagcgtt taaagacgca
    22021 ctgcgctgga acgaagtgta ttacggattt cgtcgctaag tagccacatc aggtatgtaa
    22081 cgcctgatgc gacgctgacg cgtcttatct ggcctacacg ctgcgatttt gtaggccgga
    22141 taagcaaagc gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct
    22201 acgcgctgcg attttgtaga ccggataagc gaagcgcatc cggcacgaag gagtcaacat
    22261 gttaaaagat ctcaaacgcc aggtattaga agccaacctg gcgctgccaa aacacaacct
    22321 ggtcacgctc acatggggca acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat
    22381 caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac gatatggtcg tggttagcat
    22441 cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc tccgacacgc caactcaccg
    22501 gctgctctat caggcattcc cctccattgg cggcattgtg catacgcact cgcgccacgc
    22561 caccatctgg gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc acgccgacta
    22621 tttctacggc accattccct gcacccgcaa aatgaccgac gcagaaatca acggcgaata
    22681 tgagtgggaa accggtaacg tcatcgtaga aacctttgaa aaacagggta tcgatgcagc
    22741 gcaaatgccc ggcgttctgg tccattccca cggcccgttt gcatggggca aaaatgccga
    22801 agatgcggtg cataacgcca tcgtgctgga agaggtcgct tatatgggga tattctgccg
    22861 tcagttagcg ccgcagttac cggatatgca gcaaacgctg ctggataaac actatctgcg
    22921 taagcatggc gcgaaggcat attacgggca gtaatgactg tataaaacca cagccaatca
    22981 aacgaaacca ggctatactc aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca
    23041 ggttttatct taacccgaca ctggcgggac accccgcaag ggacagaagt ctccttctgg
    23101 ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt
    23161 attcacgccg atcaggttcc ccgcgctcag catattttgc agggtgaaca aggctttcgt
    23221 ctgacaccgc tggcgttaaa ggattttcac cgccagccgg tgtatggcct ttactgtcgc
    23281 gcccatcgcc aattgatgaa ttacgaaaag cgtctgcgtg aaggtggcgt taccgtctac
    23341 gaggccgatg tgcgcccgcc agaacgctat ctgatggagc ggtttatcac ctcaccggtg
    23401 tgggtcgagg gtgatatgca caatggcact atcgttaatg cccgtctgaa accgcatccc
    23461 gactatcgtc cgccgctcaa gtgggtttct atagatattg aaaccacccg ccacggtgag
    23521 ctgtactgca tcggcctgga aggctgcggg cagcgcatcg tttatatgct ggggccggag
    23581 aatggcgacg cctcctcgct tgatttcgaa ctggaatacg tcgccagccg cccgctgctg
    23641 ctggaaaaac tcaacgcctg gtttgcaaac cacgatcccg atgtgatcat cggctggaac
    23701 gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg agcgttaccg tcttccgctg
    23761 cgtcttgggc gcgataatag cgagctggag tggcgcgagc acggctttaa aaacggcgtc
    23821 ttttttgccc aggctaaagg tcggctaatt atcgacggta tcgaggcgct gaaatccgcg
    23881 ttctggaatt tctcttcatt ctcgctggaa actgtcgctc aggagctatt aggcgaagga
    23941 aaatctatcg ataacccgtg ggatcgaatg gacgaaattg accgccgttt cgccgaagat
    24001 aaacctgcgc tggcaactta taacctgaaa gattgcgagc tggtgacgca gatcttccac
    24061 aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg tgaacggcct gccggtggat
    24121 cggcacggcg gttcggtggc agcgtttggt catctctatt ttccgcgaat gcatcgcgct
    24181 ggttatgtcg cgcctaatct cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg
    24241 atggattcac ggccagggct ttatgattca gtgctggtgc tggactataa aagcctgtac
    24301 ccgtcgatca tccgcacctt tctgattgat cccgtcgggc tggttgaagg catggcgcaa
    24361 cctgatccag agcacagtac cgaaggtttt ctcgatgcct ggttctcacg ggagaaacat
    24421 tgcctgccgg agattgtgac taacatctgg cacgggcgcg atgaagccaa acgtcagggt
    24481 aataagccgc tttcgcaggc gctgaagatc attatgaatg ccttttatgg cgtgctcggc
    24541 accaccgcct gccgcttctt cgatccgcgc ctggcatcgt cgatcaccat gcgtggtcat
    24601 cagatcatgc ggcagaccaa agcattgatt gaagcgcagg gctacgacat tatctacgga
    24661 gataccgact cgacttttgt ctggctaaaa ggtgcacatt cggaagaaga ggcggcgaaa
    24721 atcggtcgtg cactggtgca gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa
    24781 cggctgacca gcgcattaga actggagtat gaaactcatt tctgccgttt tcttatgcca
    24841 accattcgcg gagctgatac cggcagtaaa aagcgctacg ccgggctgat tcaggagggc
    24901 gacaagcagc ggatggtgtt taaagggctg gaaaccgtgc gcaccgactg gacgccgctg
    24961 gcccagcagt ttcagcagga gctgtacctg cgtatcttcc gcaacgagcc atatcaggag
    25021 tacgtccgcc aaaccatcga caaactgatg gcgggtgaac tggatgcgcg gctggtttac
    25081 cgtaaacgcc ttcgccgtcc gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc
    25141 gccgctcgcc ttgccgatga agaaaatcaa aagcgcggtc gccccttgca atatcagaat
    25201 cgcggcacca ttaagtacgt atggaccacc aacggcccgg agccgctgga ctaccaacgt
    25261 tcaccactgg attacgaaca ctatctgacc cgccagctac aacccgtggc ggagggaata
    25321 ctccctttta ttgaggataa ttttgctaca cttatgaccg ggcaacttgg gctattttga
    25381 gcaaaaaaaa gagttcgcca gataccattt tgatgcgtga cgaatgcttt gccatccagt
    25441 accatagcgc cctttccatt cctggacctg aataacacca ctacctcata agcacggtag
    25501 cgggtggtta ttgcctgcaa ttaaagatat agagccgaac acatatgcct tttacacttg
    25561 gtcaacgctg gatcagcgat acagaaagcg aattgggact tggaaccgtt gtcgcggtgg
    25621 atgcgcgaac tgtcacttta cttttcccat ctactggtga aaaccgtctg tacgcacgca
    25681 gtgattcccc cgtgacccgc gtgatgttca accctggtga taccattacc agccatgacg
    25741 gctggcagat gcaagtcgaa gaagtaaaag aagaaaatgg cttgctgacc tatatcggta
    25801 ctcgcctgga tactgaagag tccggcgtag ccctgcgtga agttttcctt gatagcaaac
    25861 tggtgttcag caaaccgcag gaccgtctgt ttgccgggca gattgaccgt atggaccgct
    25921 ttgcgctgcg ttatcgcgcg cgtaagtatt ccagcgaaca gttccgtatg ccgtacagcg
    25981 gcctgcgcgg tcagcgtacc agcctgatcc cgcatcagct caacatcgct catgatgttg
    26041 gtcgccgcca cgcgccgcgc gtcctgctgg ctgacgaagt gggtttaggg aaaaccattg
    26101 aagccgggat gatcctgcat cagcaactgc tctctggcgc tgctgaacgt gtgctgatta
    26161 tcgtcccgga aaccttacag catcagtggc tggtagaaat gctgcgccgt ttcaacctgc
    26221 gctttgctct gtttgatgat gagcgttatg ccgaagctca gcacgatgct tacaacccgt
    26281 ttgacaccga acagctggtg atttgctcgc tggattttgc ccgtcgtagc aaacagcgct
    26341 tggaacatct ctgtgaagcc gaatgggacc tgctggtggt cgatgaagcg catcacctgg
    26401 tgtggagcga agatgcgcca agccgtgaat atcaggccat tgaacaactg gcagagcacg
    26461 tgccgggcgt tctgctgctg accgcgaccc cggaacagct ggggatggaa agccacttcg
    26521 cccgtctgcg tctgctggac ccgaaccgtt tccacgattt tgcgcagttc gttgaagagc
    26581 agaaaaatta tcgtccggtt gcggacgccg ttgccatgct gctggcaggt aacaaactga
    26641 gcaatgacga actgaacatg ctcggcgaga tgatcggcga gcaggatatc gagccgctgt
    26701 tgcaggcagc aaacagcgac agcgaagatg cccagagcgc ccgtcaggag ctggtttcga
    26761 tgctgatgga tcgccacggc accagccgcg tgctgttccg taacacgcgt aacggtgtga
    26821 aaggattccc gaaacgcgag ctgcacacca ttaagctgcc gctaccgacg cagtatcaga
    26881 cggctattaa agtctccggc attatgggcg cacgtaaaag tgcggaagat cgtgctcgcg
    26941 atatgctcta cccggagcgt atttatcagg aatttgaagg tgataacgcc acctggtgga
    27001 acttcgatcc gcgcgttgag tggctgatgg gctacctgac cagccatcgc tctcagaaag
    27061 tgctggtgat ctgcgccaaa gctgccactg cgctgcaact ggagcaggta ctgcgcgaac
    27121 gtgaaggtat tcgcgctgcg gtgttccacg aaggtatgtc gattatcgaa cgtgaccgcg
    27181 ctgccgcctg gtttgccgaa gaagacaccg gcgcacaggt actgctgtgc tcagaaatcg
    27241 gttctgaagg acgtaacttt cagttcgcca gccacatggt gatgtttgac ctgccattca
    27301 acccggatct actggagcag cgtattggtc gtctggatcg tatcggccag gcgcacgata
    27361 ttcagatcca tgtgccttat ctggagaaaa ccgctcagtc ggtgctggtg cgctggtatc
    27421 acgaaggtct ggatgcattt gagcacacct gcccgaccgg acgcactatt tacgatagcg
    27481 tatacaacga tctgattaac tatctggctt caccggatca aaccgaaggc tttgacgatc
    27541 tgatcaaaag ctgccgcgag caacatgaag cgctgaaagc acagctggaa cagggtcgtg
    27601 accgcctgct ggaaatccac tccaacggtg gcgaaaaagc ccaggaactg gcagaaagca
    27661 ttgaagagca ggatgacgat accaacctga tcgccttcgc catgaacctg ttcgatatta
    27721 tcggtatcaa tcaggacgat cgcggcgaca acatgatcgt gctgacgccg tccgatcata
    27781 tgctggtgcc ggacttccct ggcctgtcgg aagatggcat caccatcacc tttgatcgtg
    27841 aagtggcgct ggcgcgtgaa gatgcacagt ttattacctg ggagcatccg ctgatccgca
    27901 acggtctgga tctgatcctt tctggcgata ccggtagcag cacgatttca ctgttaaaaa
    27961 acaaagcgtt gccggtaggt acgctgttgg tggaactgat ttatgtggtt gaagcccagg
    28021 ctccgaagca gttgcagctc aaccgcttcc tgccaccgac gccggtacgt atgctgctgg
    28081 ataaaaacgg caacaacctg gcggcgcagg tagagtttga aacctttaac cgccagctta
    28141 acgcggttaa ccgtcacacc ggcagcaaac tggttaacgc cgtgcagcag gatgttcacg
    28201 ctatccttca actgggtgaa gcgcagatcg agaaatctgc ccgtgcattg attgatgcag
    28261 cgcgtaacga agccgacgaa aaactgtctg ccgagctgtc tcgtctggaa gctctgcgtg
    28321 cagtgaaccc gaacattcgt gacgacgaac tgaccgccat tgagagcaac cgtcagcagg
    28381 taatggaaag cctggatcag gcaggttggc gtctggatgc cctgcgtttg atcgttgtaa
    28441 cgcatcagta acggagccga aaatggggat ggaaaactac aatccaccgc aggaaccctg
    28501 gttggttatc ctgtatcagg atgaccatat tatggtggtc aacaagccga gcggtttgtt
    28561 gtcagtgccg ggtcgtctgg aagagcacaa agacagcgtg atgacgcgca ttcagcgtga
    28621 ttatccgcag gcagaatcgg tgcatcgtct ggatatggct accagcggcg tgattgtagt
    28681 ggcgctgacc aaagccgcgg agcgggagtt aaaacgccag ttccgcgagc gcgagccgaa
    28741 aaagcagtat gtggcccgcg tctgggggca tccatccccc gcagaaggtc tggtggatct
    28801 gccgctgatt tgcgactggc caaaccgccc gaaacagaaa gtctgttacg aaacgggtaa
    28861 acctgcgcag acggaatatg aagtggtgga gtatgcggcg gataacacgg caagagtggt
    28921 gttaaaaccg attaccgggc gttcgcatca attgcgtgtg catatgctgg cgctggggca
    28981 tccgattctc ggcgatcgtt tttatgcatc accagaagcg agagcgatgg caccgcgttt
    29041 gttgctacat gcagagatgt tgacgattac ccatccggcg tatggcaata gtatgacgtt
    29101 taaagcgcca gcggattttt aatcgtcacg ctggcgcagt tcggtaggcg gataaggcgt
    29161 tcacgccgca tccggcaatt gtgcgccgat tgcctgatgc gacgcgggcc gcgtcttatc
    29221 aggcctacag tttaaaactt atttaaaccc tttctgctgc tttatcagct catatgcctg
    29281 ctgaatttcc tgcgctttct gcttcgccat ctccatcatc tcaggcggca aacctttcgc
    29341 caccagctta tcgggatggt gttcactcat cagcttacgg taggcacgtt tgatggtggt
    29401 cgcatcatcc gtcggcttca cgcccagcac attacaggca tcttccagcg tcgggccacg
    29461 ctgcgcttgc tgccagttac caccgccaga ttgctgctga taaccgccgc caaactgtgc
    29521 accgccctgc atcatgcgca aaaactggtc aaactgggcg cgggagatcc ctaattcttc
    29581 agcaatgaca tacagcaccg cccgttcatt cgggtgcagt gaaccatcag caaacgccgc
    29641 ctgaatctgg atctccagaa acatacgaat taagtcaaaa cgaccaaagc agacactgcg
    29701 aaactggcgc atcttttcgc gcagcgggta attatctgat tttcccaccc ggaacgcgtt
    29761 ttgcgccgca gtacgggaag cgccatgaag gttcattcgg tccatcaact ggctggcgat
    29821 atgaatatca gcctccgtga cgcgaccttt ggatttggtt aaatgcccca tcacttcaaa
    29881 agtggtggca aaaaacagcg cctgacgctc acgctggttg gcgaaccacg ccattttacg
    29941 gctacgggct ttatcaaaca tatggccaat taacaggcct aacactacgc cccaaaagcc
    30001 gccgcccatc agtaaggcca cggccacgcc aatgattttt ccccaatact gcatatattc
    30061 cccaaatcga cacacggata tcagggctat ctcccacaat ataaaggtgc ttttaccgtt
    30121 ttccggctgc ggtcaattgt gggacatcgc ctataatttg cattatcata cctgtcattc
    30181 aatgccgtgc ctaacaccac tgacgctatt cggacaggat taacactagc gtagagatga
    30241 cgagtacgtt agtctctgac cgtttgtcac gcgcaacgtt accgatgatg gaacaataaa
    30301 atcaacgtat gaaaaaacgt atccccactc tcctggccac catgattgcc accgcccttt
    30361 atagtcaaca gggactggca gccgacctcg cctcacagtg catgttgggc gtgccaagct
    30421 atgaccgtcc tctggtacag ggcgatacca atgacttacc cgtgactatc aatgctgacc
    30481 acgcgaaagg ggactacccg gatgacgccg tgtttactgg cagcgtggat atcatgcagg
    30541 gtaacagccg tctgcaggcc gacgaagtgc agctccatca aaaagaggca ccaggacaac
    30601 cggagccggt acgtaccgtt gatgcgctcg gtaatgtcca ttacgacgat aaccaggtga
    30661 tcctcaaagg gccgaaaggc tgggcgaatc tgaacaccaa agataccaac gtctgggaag
    30721 gtgattacca gatggtgggt cgccagggtc gcggtaaagc ggacctgatg aaacaacgtg
    30781 gcgaaaaccg ctataccatt ctggataacg gtagctttac ctcctgtctg ccgggttctg
    30841 acacctggag cgtggtaggt agcgaaatta ttcatgaccg cgaagaacaa gttgcggaga
    30901 tctggaacgc ccgctttaag gtgggtccgg taccgatctt ttatagcccc tatttgcagt
    30961 tgccggtggg tgacaaacgt cgctctggtt tcttgatccc gaacgccaag tacaccacca
    31021 ccaactactt tgagttctac ctgccatatt actggaacat cgcgccaaat atggatgcca
    31081 ccatcacgcc gcattatatg catcgtcgtg gcaacatcat gtgggagaac gaattccgct
    31141 acctctccca ggcgggcgct ggcttgatgg aactggacta tctgccttca gataaagtct
    31201 atgaagatga acacccgaac gatgacagtt cacgtcgttg gttattctac tggaaccact
    31261 ccggggtcat ggatcaggtg tggcgtttca acgtcgacta caccaaggtc agcgatccta
    31321 gctacttcaa tgatttcgat aacaagtacg gttccagtac tgacggctac gcaacgcaaa
    31381 aattcagcgt tggctatgcg gtgcaaaact tcaatgccac cgtttcaacc aagcagttcc
    31441 aggttttcag cgaacagaac accagtagct actcggcaga gccgcagtta gacgttaatt
    31501 actaccagaa tgatgttggt ccgtttgata cgcgtattta cggccaggca gtgcactttg
    31561 ttaacaccag agacgacatg cctgaagcaa cccgtgttca cctggaaccg accatcaatt
    31621 tgccgctctc taataactgg ggcagcatca ataccgaagc gaagtttctg gcaacccatt
    31681 atcagcaaac caatcttgac tggtataact ccagaaacac gaccaagctg gacgaatccg
    31741 ttaaccgcgt aatgccgcaa ttcaaagttg acggcaaaat ggtctttgaa cgcgatatgg
    31801 aaatgctggc tccgggttat acccaaacgc tggaaccgcg cgcgcagtat ttgtacgtgc
    31861 cgtatcgcga tcagagcgac atctataact acgactcgtc tctgctgcaa tctgactact
    31921 ctggcctgtt ccgggaccgg acttacggcg gtcttgaccg tattgcctcc gctaaccagg
    31981 tgacgaccgg tgtcacatct cgcatatatg atgatgctgc cgttgaacgt tttaatattt
    32041 ccgttggtca aatctactat ttcacggagt ctcgcactgg cgatgacaac ataacatggg
    32101 agaatgacga caaaacgggc tcactggtgt gggcaggcga cacctactgg cgtatctccg
    32161 agcgttgggg attgcgtggc gggattcagt acgatacacg tctggataac gtagcgacca
    32221 gtaactccag cattgaatac cgtcgggatg aagaccgtct ggtacagctg aattaccatt
    32281 acgccagccc ggaatatatt caggctacgc tgcctaagta ctattccact gctgagcaat
    32341 ataagaatgg tatttcgcag gtaggtgctg tcgccagcag gccaattgcc gatcgttggt
    32401 ccattgttgg ggcctactac tacgacacca atgcgaacaa gcaagccgac tctatgttag
    32461 gtgtgcaata cagctcctgc tgctatgcaa ttcgcgtcgg ttacgagcgg aagctgaacg
    32521 gttgggataa cgataaacaa catgcggtat atgacaacgc aatcggcttt aacatcgaac
    32581 ttcgcggcct gagctccaac tacggtctgg gtacgcaaga gatgctgcgt tcgaacattc
    32641 tgccgtatca aaacactttg tgatttgttg atttaccacg taatccgcag tgcggttaat
    32701 tgaaatggaa aaagtatgaa gaactggaaa acgctgcttc tcggtatcgc catgatcgcg
    32761 aataccagtt tcgctgcccc ccaggtagtc gataaagtcg cagccgtcgt caataacggc
    32821 gtcgtgctgg aaagcgacgt tgatggatta atgcagtcgg taaaactgaa cgctgcgcaa
    32881 gcaaggcagc agcttcctga tgacgcgacg ctgcgccacc aaatcatgga acgtttgatc
    32941 atggatcaaa tcatcctgca gatggggcag aaaatgggag tgaaaatctc cgatgagcag
    33001 ctggatcagg cgattgctaa catcgcgaaa cagaacaaca tgacgctgga tcagatgcgc
    33061 agccgtctgg cttacgatgg tctgaactac aacacttatc gtaaccagat ccgcaaagag
    33121 atgattatct ctgaagtgcg taacaacgag gtgcgtcgtc gcatcaccat cctgccacag
    33181 gaagtcgaat ccctggcgca gcaggtgggt aaccaaaacg acgccagcac tgagctgaac
    33241 ctgagccaca tcctgatccc gctgccggaa aacccgacct ctgatcaggt gaacgaagcg
    33301 gaaagccagg cgcgcgccat tgtcgatcag gcgcgtaacg gcgctgattt cggtaagctg
    33361 gcgattgctc attctgccga ccagcaggcg ctgaacggcg gccagatggg gtggggccgt
    33421 attcaggagt tgccggggat cttcgcccag gcattaagca ccgcgaagaa aggcgacatt
    33481 gttggcccga ttcgttccgg cgttggcttc catattctga aagttaacga cctgcgcggc
    33541 gaaagcaaaa atatctcggt gaccgaagtt catgctcgcc atattctgct gaaaccgtcg
    33601 ccgatcatga ctgacgaaca ggcccgcgtg aaactggagc aaattgctgc tgatatcaag
    33661 agtggtaaaa cgacttttgc tgccgcagcg aaagagttct cgcaggatcc aggttctgct
    33721 aaccagggcg gtgatctcgg ctgggctaca ccagatattt tcgatccagc cttccgtgac
    33781 gccctgaccc gcctgaacaa aggtcaaatg agtgcaccgg ttcactcttc attcggctgg
    33841 catttaatcg aactgctgga tacccgtaat gtcgataaaa ccgacgctgc gcagaaagat
    33901 cgtgcatacc gcatgctgat gaaccgtaag ttctcggaag aagcagcaag ctggatgcag
    33961 gaacaacgtg ccagcgccta cgttaaaatc ctgagcaact aatggttaaa acccaacgtg
    34021 ttgtgatcac tcccggcgag cccgccggga ttggcccgga cttagttgtc cagcttgcac
    34081 agcgtgagtg gccggtcgaa ctggttgttt gtgccgatgc cactctcctt accgaccggg
    34141 cagcgatgct cggtttgccg ctcaccctcc gcccttattc ccccaactcc cctgcacaac
    34201 cgcaaactac gggcacatta acgctacttc ctgtcgcgct acgtgaatct gtcactgcgg
    34261 ggcagttagc gattgaaaat gggcattacg tggtggagac gctggcgcga gcgtgtgatg
    34321 gctgtctgaa cggtgaattt gctgcgctga tcacaggccc cgtgcataaa ggcgtcatta
    34381 acgacgcaag cattccgttt accggtcata ccgagttttt cgaagagcgt tcgcaggcga
    34441 aaaaagtggt gatgatgctg gcgaccgaag aacttcgcgt ggcgctggca acgacgcatt
    34501 taccgctgcg cgatatcgca gatgctatca cccctgcact tttgcacgaa gtgattgcta
    34561 ttttgcatca cgatttgcgt acaaaatttg gtattgccga accgcgcatt ctggtctgcg
    34621 ggctgaatcc gcacgcgggc gaaggcggtc atatgggtac ggaagagata gacaccatta
    34681 ttccggtgct cgacgagctg cgggcgcagg ggatgaaact caacgggccg ctgcctgccg
    34741 ataccctgtt tcagccgaaa tatctcgata acgccgacgc cgtgctggcg atgtaccacg
    34801 atcagggtct tcccgtgcta aaataccagg gcttcgggcg cggtgtgaac attacgctgg
    34861 gcctgccctt tattcgcaca tcagtggacc acggcaccgc gcttgaactg gcgggacgtg
    34921 gcgaagccga tgtcggcagt tttattacgg cgcttaatct cgccatcaaa atgattgtta
    34981 acacccaatg aataatcgag tccaccaggg ccacttagcc cgtaaacgct tcgggcaaaa
    35041 ctttctcaac gatcagttcg tgatcgacag cattgtttct gccattaacc cgcaaaaggg
    35101 ccaggcaatg gtcgaaatcg gccccggtct ggcggcattg accgagccgg tcggcgaacg
    35161 tctggaccag ctgacggtca tcgaacttga ccgcgatctg gcggcacgtc tgcaaacgca
    35221 tccattctta ggcccgaaac tgacgattta tcagcaggat gcgatgacct ttaactttgg
    35281 cgaactggcc gagaaaatgg gtcagccgct gcgtgttttc ggcaacctgc cttataacat
    35341 ctccacgccg ttgatgttcc atctgtttag ctatactgat gccattgccg acatgcactt
    35401 tatgttgcaa aaagaggtgg tgaatcgtct ggttgcagga ccgaacagca aagcgtatgg
    35461 tcgattaagc gtcatggcgc aatactattg caacgtgatc ccggtactgg aagttccgcc
    35521 gtcagccttt acaccaccac ccaaagtgga ttccgccgtc gtgcgcctgg tccctcatgc
    35581 aacgatgcct cacccggtta aagatgttcg cgtgttgagc cgcatcacca ccgaagcctt
    35641 taaccagcgt cgtaaaacca ttcgtaacag cctcggcaac ctgtttagcg tcgaggtgtt
    35701 aaccggaatg gggatcgacc cggcgatgcg agcggaaaat atctctgtcg cgcaatattg
    35761 ccagatggcg aactatctgg cggagaacgc gcctttgcag gagagttaac gatgatcaat
    35821 tcgccccgag tgtgtattca ggttcaaagc gtctacattg aggcacaatc ttcacctgat
    35881 aatgaacgtt acgtttttgc ttataccgta accatacgca atctggggcg agcgccagtg
    35941 cagttgttgg ggcgttactg gctgatcacc aatggcaatg gccgtgaaac cgaagtccag
    36001 ggcgaaggag tggttggcgt ccagccactt atcgcgcctg gcgaagagta ccagtacacc
    36061 agcggtgcaa tcattgaaac cccgctgggc accatgcagg gtcactacga aatgatcgat
    36121 gaaaatggcg tccctttcag catcgacatt cccgtattcc gactcgccgt tcccacactc
    36181 attcattaaa agaatatggc gacatacctt attggcgacg ttcatggttg ttacgatgaa
    36241 ctgatcgcat tgctgcataa agtagaattt acccccggga aagataccct ctggctgacg
    36301 ggcgatctgg tcgcgcgcgg cccgggttcg ctggatgttc tgcgctatgt gaaatcctta
    36361 ggcgacagcg tacgtctggt gctgggcaat cacgatctgc atctgctggc ggtatttgcc
    36421 gggatcagcc gcaataaacc gaaagatcgc ctgacaccgc tgctggaagc gccggatgcc
    36481 gacgagctgc ttaactggct gcgtcgccag cctctgctgc aaatcgacga agagaaaaag
    36541 ctggtgatgg cccacgcagg gatcacgccg cagtgggatc tgcagaccgc caaagagtgc
    36601 gcgcgcgatg tagaagcggt gctgtcgagt gactcctatc ccttctttct tgacgctatg
    36661 tacggcgata tgccaaataa ctggtcaccg gaattgcggg ggctgggaag actgcgtttt
    36721 atcaccaacg cctttacccg tatgcgtttt tgcttcccga acggtcaact ggatatgtac
    36781 agcaaagaat cgccggaaga agcccctgcc ccactgaaac cgtggtttgc gattcctggc
    36841 cctgtcgctg aagaatacag catcgccttt ggtcactggg catcgctgga aggcaaaggt
    36901 acgccggaag gtatttacgc gctggatacc ggctgctgct ggggtgggtc attaacctgc
    36961 ctgcgctggg aagataaaca gtattttgtc cagccgtcga accggcataa ggatttgggc
    37021 gaagcggcgg cgtcttaaac acagcctgat acaggaaggc cggataagac gcgaccggcg
    37081 tcgcatccgg cactagccgt aaattctata caaacttacc gccgctccag aatctcaaag
    37141 caatagctgt gagagttctg cgcatcggcg tcgtggaatt cgctgaatac cgattcccag
    37201 tcatccggct cgtaatccgg gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc
    37261 agatacagtt tttgcgcttt tggcaggaac tgctcataaa cgcgaccgcc gccaatcacc
    37321 atgatttctg gtacgtcacc acacgccgca atggcttcat ccaccgattt cacccacgtt
    37381 acgcgatcgt ccgtacccgg ttgactgctg aggataatat ttttgcgtcc tggcaacgga
    37441 cgaccgattg attcccaagt atggcggccc ataatcacgg gtttatttaa ggtgttgcgt
    37501 ttaaaccagg cgagatcggc aggcaggttc cacggcatgg cgttttccat gccgataacg
    37561 cgatctaccg ctaacgccgc aatcagactg atcattgaga tttcccgata aaaaaattgt
    37621 cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa
    37681 ttttatattc tgctggcgag tccacgctct ccccctggac tcgccgcatt acaatgaaac
    37741 aaaaacaaac agttagctgt aaagtgtgat ttacgtcact ctttattaag atgagggttt
    37801 cgtttccggt tcatccgcca tgttgccggt atgtttacct tcttctgttc cctgccagcc
    37861 atgtcgttga attaatgaca gatgttcgcg gtcctcggtg atgatctcac ttaacatcgc
    37921 gctggtgcgt ttatagaccg ccgcgcgggc tttggtgtca ttctcaacca ttgccatctc
    37981 ttccaccatc tgaatattaa agcggcggaa cacatcggca cgttctcgcg cttcatacgg
    38041 ccccagacct aaactttcca gcgccagacg cccggttttc agcgcacctt caaaggtttc
    38101 acgctccggc ttttcaacgc ctgcctgacg caaacgaatg tagtgatcga catcgcgggc
    38161 gcgggcaata atctgcaaat gcgggaaatg ttctttcacc atctccgtca gttgcaggtt
    38221 ggtttgcgga tcgtcaatag cgttaatcag cacttccgct ttcgccgctc cggcagattc
    38281 cagtaaatcc atccgcgtgg catcgccata aaacactttc ataccaaatt tacgcagcgt
    38341 ttcgatatgg tccggatcgt gatcgagcac caccattttc accccgctgg agagcagtaa
    38401 acgtccggta atctgtccaa aacgaccgaa tccggcgata atcacgcgcg gctgttcttc
    38461 atcgatctca tcggcttcac gcgcctcctc cgtagaagat tgctcaaggc gattgaggat
    38521 caccagcaga atcggcgttg ctgccatcga cagcgccacc gccagggtca gcgatttcgc
    38581 ccactccggc tccagcacat tcgccatctg cgccgcacca aataccacaa aggcaaactc
    38641 actgccctgc cctaacaaca ccgcaaacca gcgacgctgt ttatttggca cctgcaacgg
    38701 tcgggcaatc agccacagca tggcgatttt gatgatgagg aaaccgagca gcaaaatgac
    38761 aatgcgcaat gggttttcaa tcagcgtgcc aaagtctatc gacatgccaa caccgatgaa
    38821 aaacagcccc aacagcaaac ctttaaacgg ttcgatatcg ctttccaacg catgacggta
    38881 ttccgagctt gccagcagta cgcccgccag aaacgcgccc atcgccatcg acaagccgac
    38941 ctcttccagc agcaaaccaa agccaaacac aaggaataac gccacggcac taaacacttc
    39001 ccgcaagcca gagcgggcaa caaaacgcag cgccggacgc gtaacatagc gccccagcaa
    39061 taccaccagt accagcgcac ccgccacttt taacgccgag agagcaaatg cgcccatcgt
    39121 cgtcgaggca ctgctcgccg ccagtagcgg gatcatcgcc accagcggga ttgccgcgat
    39181 atcctggaac agcagcactg caaaggcact gcggcccatt tgcgtcacca tcagattgcg
    39241 ttcattcatc gcctgcatgg caatcgccgt tgatgagagc gccagcgtca taccgatcaa
    39301 ttccgcgacc tgccagcgca gcccaagtaa catacagaac agccccagca ggccaccgca
    39361 aatcaccatc tgcaatgcgc caccgccgaa cactgccgca cgcagcttcc acagcctttg
    39421 tggatcgagt tcgaggccga taataaacag catcagcacc accccaatct cggcaaagtg
    39481 cagaatagat tcggcatcgg tcaccagtcg cagcccccac gggccaataa tgcagccggc
    39541 gatcaggtag ccaagtaccg atcccagacc aagacgtacc gcaatgggta caatcagcgc
    39601 cgccgatccg aggtagatca gcgcctgaac cagcgtatga ctatccatga tgggcctcct
    39661 gccattccag cagacgttgc ttatagtggc gcgcctgccc ttcgagggtt tcgtcgtcac
    39721 aaataaaggt gcagtgcatg gcaaacggtg gcagccagtt cagcccgcag tagattgccg
    39781 tcgcctgtag cggctgcgac agcacatcaa agcccggatg cgcaccaatt tcaaaatggc
    39841 tttccccgcc gccggtcgtc accgcccaca gcaaatgttt gccatgcagc gccgtgccgc
    39901 cgtgaccgta agcccagccg tgcgacaaaa ctttatcgat ccaaagttta agaagcggag
    39961 gaatgctgta ccactgcatc gggtgctgcc agacgatcag atcggcgcga gagagcgcct
    40021 cctgctcggc ggcaatatcg atattgaagt caggatagag ttgataaaga gagcgaattt
    40081 cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg
    40141 gatacggatg cgcataaatg atgaggatca tagttattag cccgcgtgtt tacgcctttg
    40201 ttttcccaca aagtgtagtc atatattcac gcggcttata gtcaataata ttgattcaga
    40261 aaagagagaa atctgaacaa ataatcccat tctctgcccg cggatcgtca tattgctggt
    40321 ctgcgccagt gacattcctc gagtctccgg ggcaaacgct acggaaatca acaagccaaa
    40381 cagcgagata cccgccccca ttagcatcgt gttactgata ccgtaattat tgataaatat
    40441 cggcagtgcc caggtcgaaa caatggtgcc aatacgactt aaggacataa tcacgcccac
    40501 ggcagaggcg cggatatccg tcgggaagag ttcattagga tagagccact gcaaattacc
    40561 cggcccgcca gagaaaaagg catacaccgc aaaggccatc actaccagcc agatccccat
    40621 atccgggatt agccccaaaa ccgccagcgc cagcgtcatc atggcaaagc tgccaatcaa
    40681 caatggacgc cgtccggcag tgtttagcca cagcatcggc ggaatacagc cgagcataaa
    40741 gaacaggcta atcaccacat tccctagtgc cgcgtttttg ccaaccccca atcccaacga
    40801 accaacgatt tgcgggccaa aggtgtaaat ggcgaacatt gggatcacct ggcaggtcca
    40861 gatggcggca acaaacagaa caaaaggaaa atggcggcga ttaaacagat cgcgaaaacg
    40921 ggtttgctgc ggctgctctt catcgaaagc caccggttcg ccaaacagtt ttatcatcat
    40981 ctcttcgcac tcttttactc gccctttgcg taataaccag cggggagatt ctggcagttc
    41041 gaatcgacca atcaaaatca acaaacaagg gatcgccgcg ctacccagca tccagcgcca
    41101 accaccttcc acatcataaa gccagtagcc gaccagatcg gcgcaggtcg cgccgacata
    41161 ccacatcgcg gcaataaagc tgatggaaaa cgcccgctga cgggtactgg agaactcggt
    41221 gatcatcgaa gtggcgatgg gataatctgc accgatgaca atgccgatga gtacccgcat
    41281 caccaacagt tcgacggggg atgaaacaca catcgtcgcc accgatatca cgccgatggc
    41341 gatgatatca atgaggaaca ttttgcgccg tccgacttta tcggagatat agccaaacag
    41401 cgatgtgcca acgaacagcc cggcgagcgt tcccgcgccc agcaagccaa tccagtcagc
    41461 gtccagtttc agcgccggag tcagttgctc cagcgccacg ccaatcatta ccagtacata
    41521 accatccaga aacggaccac cgcttcccca cagcaaaatg cggcggtgaa tagaggagaa
    41581 tttgagatcg tcaaagtttc tggacggttg catgggttat tcctgttttt ttgcaatgag
    41641 agtcagtacg gcgttacgta tcaaaccaac atcagccgta acggaactcc acaccaaagg
    41701 tgccgcgcgg gtattcccac tgttccagcg ccgaccccag ccccagaatg cgacaggtgc
    41761 cgcactccag acatccggcg taatcgaagc gcacactgcc gtcatcctgc ttcttgtaca
    41821 gacctgcggg gcacgctttc accagcagct ccagcacctg tttatcagca tcggccttca
    41881 caacaatgtg cggatgctct tcatcgacat tgaatttatt gacgcccagt ttgacgtcca
    41941 cattgacggg agaagtcata aaacggtcac tcctttcatg ccatccttga tcagattgat
    42001 gaagcccact ttcttgccgt ggcggaggat tttcttgcgc atcagttccg gcgcactgcc
    42061 gtcaatggtg aacaggtcac gcgccacgcc caccgccagt tccgggttgc cgctaaacat
    42121 gcgtgggtta tcaaggaacg ccggtagttt ctggtacatg cgcatatcgc gcagcgggcc
    42181 actctccaga tgctgacgat attccgcgag tttttgctta ctgaaatcgt cgcttttcat
    42241 cgctgaaagc acggtttttg ctgcagcttc cccggcggca atcgccagat ccataccgcg
    42301 aatggtaaaa ccgaggttca tacacattcc ggcggcatca ccggcaatca atacgccgtc
    42361 accgaccagc tccggcagca tgttgatgcc tgcttccggc actacgtgag cggaatattc
    42421 caccagcttg ccgcccgcga tcagcggtgc aacggccgga tgctgtttga aatcttccag
    42481 catttgcggc accgattttt tcgcgtcatg cagatgatgc agaccacaaa ccagccccag
    42541 cgacagggta ttttcattgg tataaaggaa gccgccgccc atcaggccat cggtgggtga
    42601 tcccgcaaac agacaagccg cgccctgatt accctgcaac tgaaaacggt cttcgattac
    42661 cgacttcggt aactcgatca gttccttcac gccaaccgcc acatccgtcg gtttaacgcg
    42721 ttttgccatc cccaactttt cggcaaggat ggagttcacc ccatcagcaa ggagcaccgt
    42781 tttcgcttca atcacatcgc catcggcttc tacaccgacg actttgccat cgcgctgtac
    42841 gaggttatcg acgcggatcc cggtaattaa ctgcgcgccc gcttcttcgg cctgctccat
    42901 caaccaggca tcaaatttac tgcgcaaaac ggagtaagaa cgctgggatg gcgaggtttc
    42961 gtcaccgttg cagtagtcca tagtcatcgc tgacttttcc gtcataaacg cgagtttttc
    43021 atgggtgatc aggcgttcta cgggggcgga atcggcgaaa cctggaataa tgtgttccag
    43081 actgtgggca tagagacgcc cgccagtaac gttcttggca cctgcggaat tgccacgctc
    43141 gataactaac acttgcgccc cttcgcgggc gagcaccagt gcggcaaccg aaccggcaag
    43201 ccctgcaccg acgatgatgg cgtcaaagat atcttcggac ataagagctc cctgtcagcg
    43261 gcagagccgt aatgcagccc tgccagagtg gatcaacgcg ctaaagctgc ggtcagtgcc
    43321 ggaaggatct tcacggcatc gccaacaatg ccgtaatccg cgtactggaa gatcggcgca
    43381 tttttatctt tattgatggc gaaaatggtt tgcgacgcgt tagcgccaac catgtgctgg
    43441 atctacccgg agatccccac cgccaggtac agttcaggtt tcagcatcag gttggagata
    43501 ccgacatagc gttcgtgctc catccatttt tcgttttctg ccaccggacg agaacaggct
    43561 aactccgcac ctatcgcctt gcaaagctgt tctgccagcg caatgttctc tttgctgcca
    43621 atgccgcgac cgacgctgac caccagacgg gctttgtcga gatcgacgct gttgctctgg
    43681 cgcgcctggg ttgccgtgcg ggtaatcgcc acagccggag cctgccactc cacggtgtgc
    43741 gtttcgccag tgcgtgacgc gtctggctga gccgcatcga acgtgccgct gctgatggtc
    43801 agtaccgcat acggcgtggc aatgcgttct tcgccaatcg ccagaccgcc gtaaaccatg
    43861 tgtttcactg ttgctttacc gtcctgtacg ctgacggtgc tggcatcgtt agacaccgcc
    43921 gctttaaggc gataacccag ttttgccgcc agtaatttgc cgcgacgggt gtttggcaac
    43981 agcaccagac cgtcagcgcc gtgctggcga atagtgtcag ccatgacacc ggcgtaatct
    44041 tcgatcatcc gatcgtccgg tttgccgttt aatttccaga catgattagc gccgagctgg
    44101 attgcctgtg cgccgtcggc atcattgagg acaaaggcat tgatttgatt agctaaagcc
    44161 tgcgcaccgt tcatcagttc cggcagacga gagggggtat cgctgaatac ccagacttga
    44221 gaaaacgtgt tcatagcatc ccctgtaatt aaatgacttt gcgaagattt tcagcaaatg
    44281 cggcgatctg ttcttcgccg tcgccttcaa tcacgatgcg ctgacgctcg cgctgtttcg
    44341 gcgcggcaac ctgttgttca gaccaggcgt ccacggcgtt aaaaccaata tccgccgccg
    44401 accatacctg gactggcttt ttcgccgcgc cgagaatggc tttcatcgaa ggaatttgtg
    44461 gggagttgat atcagtggaa acagcaacaa ccgcaggcag cggaatgctt aaggtttcgg
    44521 tttcatcttc cagttcgcgc tcaacggtga aggtatctgc cgtcagggag ataattttgc
    44581 tgacgccgtt aactgccgga atattgagga tttcgcccac cagcagacca acctgctggg
    44641 cataaaggtc ggaagaacca tcgccacaga ggatcagatc aaagcctgct ttctgggcgg
    44701 ctgcagccag tgcgctcgcc gtttgttgcg gcagtgcctg ctcgaactgg tcatcaatca
    44761 ccacaatcag ttcatccggg ccgcgcgata gcacatcttt acgccctttg gcgttggtca
    44821 gggctttacc gcccacactt aaggctgtca cctgcgcctc tgctgcctgt tgctttagct
    44881 ggcaagccgc ttcaatagcg ttgagatcgt attggcttat tttggcatcg gctttgctga
    44941 agtctaatga accatcagca ttattgaccg caatatcctg ttcatcaggc acgcacttat
    45001 agcaagtaat aatcttcatt gcatctccag aaatcatgaa ggaaacgtcg ttcatatcgg
    45061 tggtgttaat tacagatatc acgcccttta atttggaaat aaaactttaa tcaccaatat
    45121 tgaaaatgtc acacgcagat aaatccaact ttcaatattg ttaagttcct caccaatatt
    45181 gaaaacatgg cgtagcaaaa agaaattttc aatattgttt tatagatcac caatattgaa
    45241 agtcagatgc tattttttta cccacaaaag aatataacaa actgattaat aaccaaaatt
    45301 aacgaaaaac gccctctgtt cactcgaaac aaaaatgtga caccaatcac agaatacagc
    45361 ttattgaata cccattatga gttagccatt aacgcgtcca cgaggttaat aataattata
    45421 ttaaatgtta acaaaaataa aacaaacggg aacgcaaaat aaatattcgt tttcacagtg
    45481 gaattaactc atgaagaatg aaaagagaaa aacgggaata gaaccgaagg ttttctttcc
    45541 gccattaata atcgtcggca tactttgttg gcttacagtc agagatctgg atgcggcgaa
    45601 tgtcgttatt aatgctgtat tcagttacgt caccaatgta tggggatggg catttgaatg
    45661 gtatatggtg gtgatgcttt tcggttggtt ctggctggtg tttggcccgt atgccaaaaa
    45721 gcgtttaggt aacgaaccgc cagaatttag caccgccagt tggatcttta tgatgttcgc
    45781 ctcctgtacg tctgctgccg tactgttctg gggatcgatt gagatctact actacatctc
    45841 caccccgccg tttggcttag aaccgaactc gacaggagcg aaagagttgg ggctggctta
    45901 cagcttgttc cactggggac ctctgccgtg ggccacttac agcttccttt cagtcgcctt
    45961 cgcttacttc ttctttgtcc gcaaaatgga agtgattcgc cccagctcga cactggtgcc
    46021 gctggtaggt gaaaaacacg ccaaagggtt gttcggtacc atcgtcgaca acttctatct
    46081 cgtcgccttg atcttcgcga tgggtaccag tctgggcctt gccacgccgc tggtgaccga
    46141 gtgtatgcaa tggttgtttg gcattccgca taccctgcaa ctggacgcta tcatcattac
    46201 ctgctggatt atcctcaacg ccatttgcgt cgcttgcggt ctgcaaaaag gggtacgtat
    46261 cgccagtgac gtgcgtagtt acctgagctt cctgatgctg ggttgggtgt tcatcgtcag
    46321 cggtgccagc ttcatcatga actacttcac cgattcggtg gggatgttgc tgatgtatct
    46381 gccgcgcatg ttgttctata ccgatcccat cgctaaaggc ggcttcccgc agggctggac
    46441 cgtgttctac tgggcatggt gggtgattta cgccatccag atgagtatct tcctcgcccg
    46501 catctcccgt ggtcgtactg tgcgtgaact gtgcttcggc atggtgatgg ggctgacagc
    46561 atccacctgg atcctgtgga ccgtgctcgg tagtaacact ctgctgttga tggataaaaa
    46621 catcatcaac attccaaatc tgatcgaaca gtacggtgtg gcgcgcgcca tcatcgaaac
    46681 ctgggccgct ctgccactca gcaccgccac catgtggggc ttcttcatcc tctgctttat
    46741 tgccaccgtt acgctggtta acgcctgctc ttataccctg gcgatgtcta cttgccgcga
    46801 agtacgcgat ggtgaagaac caccgctgct ggtgcgtatc ggttggtcaa ttctggttgg
    46861 cattatcggt attgttctgc tggcgctcgg cggcctgaaa ccgattcaaa ccgccattat
    46921 cgccggagga tgcccgctgt tcttcgtcaa cattatggtg acgctctcct ttattaaaga
    46981 cgcgaaacag aactggaaag attaattaac ccccaaaata tcaagaggtt gaaagatgga
    47041 ttttaattta aatgatgagc aggaactgtt tgtcgccggt atccgcgaac tgatggccag
    47101 cgaaaactgg gaggcctatt ttgccgagtg cgaccgtgac agcgtctacc cggaacgttt
    47161 tgtcaaagca ctggcggata tgggtatcga cagtctgctg atccctgaag agcacggtgg
    47221 tctggacgcg gggtttgtta ctctcgccgc cgtgtggatg gagctgggac gtctgggggc
    47281 accaacctat gtactgtacc agctgccggg cgggttcaat accttcctgc gcgaaggcac
    47341 acaagagcag atcgacaaga ttatggcttt ccgcggcacc ggtaagcaga tgtggaactc
    47401 agcgattacc gaaccgggcg cgggctccga cgtgggtagc ctgaaaacga cttatacccg
    47461 tagaaatggt aagatttatc ttaatggtag taagtgtttt attaccagta gcgcctacac
    47521 cccgtacatc gtggtgatgg cgcgcgacgg ggcttctccg gacaaacctg tctacaccga
    47581 atggtttgtt gatatgagca aaccgggcat caaagtgacc aaacttgaga agctcggtct
    47641 gcgtatggat agctgctgtg aaatcacctt tgacgatgtg gaactggacg agaaagacat
    47701 gttcggtcgg gaaggtaacg gctttaaccg cgtcaaagaa gagttcgacc atgaacgttt
    47761 cctggtagcc ctgaccaact acggtacggc gatgtgcgcc tttgaagatg cggcgcgcta
    47821 cgccaaccag cgcgtgcagt ttggcgaggc tattggtcgt ttccagttga ttcaggaaaa
    47881 attcgcccac atggcgatca aattaaactc catgaaaaac atgctgtatg aagcagcgtg
    47941 gaaagcagac aacggcacca tcacctctgg cgatgcagcg atgtgcaaat acttctgcgc
    48001 caatgcggca tttgaagtgg tggatagcgc aatgcaggtg ctgggcggtg tcgggattgc
    48061 gggcaaccac cgcatcagcc gcttctggcg tgacctgcgt gtagaccgcg tttccggagg
    48121 atctgacgaa atgcagatcc tgacgctggg tcgtgcggtg ctgaagcaat accgctaagt
    48181 ccgtgcgctg cctgatgcga cgctgacgcg tcttatcagg cctacgggag cacgaatgta
    48241 agccggataa ggcgtttacg ccgcatccgg cagtcatgcg ctacataaat ttttcaggag
    48301 ctaataatgg atcatctacc catgccgaaa ttcggaccgc tggccggatt gcgcgttgtc
    48361 ttctccggta tcgactgacg atacggcgga aattctcttc acctccggca ccacctcccg
    48421 accgaaaggt gtggtgatta cccattacaa cctgcgcttc gctggatatt actccgcctg
    48481 gcagtgtgca ctgcgtgacg atgacgtcta catgacggta atgcctgcgt ttcatatcga
    48541 ttgccagtgt actgcggcga tggcggcgtt ttctgccggg gccacctttg tgctggtcga
    48601 gaaatacagc gcccgcgcct tctggggaca ggcgcagaag taccgcgcca ccattaccga
    48661 atgtattccg atgatgatcc gtacgttgat ggtgcagccg ccttcagcga acgatcggca
    48721 acaccgcctg cgggaagtga tgttttatct caacttgtcg gagcaggaaa aagacgcatt
    48781 ttgtgaacgc ttcggcgttc gcttgctgac gtcttatggg atgacggaaa ccattgtggg
    48841 cattatcggc gatcgcccta gcgataaacg acgctggccg tcgattggtc gggcggggtt
    48901 ttgctacgaa gcggagatcc gcgacgatca caatcgcccg ctcccggcag gtgagatcgg
    48961 tgaaatctgt attaaaggcg taccagggaa aaccatcttc aaagagtatt ttctcaaccc
    49021 gaaagccact gcgaaagtgc tggaagccga cggctggcta cataccggcg ataccggata
    49081 ccgcgacgaa gaaggctttt tttatttcgt cgatcgccgc tgcaatatga tcaaacgcgg
    49141 tggcgagaat gtctcctgcg tggagctgga aaatatcatc gccacccatc cgaaaattca
    49201 ggacatcgtg gttgtgggta ttaaagattc gattcgcgat gaagccatca aagcatttgt
    49261 ggtgctgaat gaaggtgaaa cattgagcga agaggaattt tttcgcttct gcgaacaaaa
    49321 tatggcgaaa tttaaagtgc cctcttatct ggagatcaga aaagatctgc cacgtaattg
    49381 ctcggggaaa ataattagaa agaatctgaa ataaatgaaa cgtcagggaa ctaccctgcc
    49441 agctaataat cacgccctaa aacaatatgc attttttgca gggatgttgt cgtccctaaa
    49501 aaagcaaaaa tggagaaaag gaatgagtga atcattacat ctgacccgca atggatcaat
    49561 tctggaaatt acccttgatc gcccaaaagc gaatgctatt gatgcaaaaa ccagctttga
    49621 aatgggcgaa gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg ccattattac
    49681 cggtgccgga gagaaattct tttccacggg ctgggattta aaagcggcag cagaaggcga
    49741 agcaccggat gctgactttg gcccgggtgg ttttgcggga ttaaccgaaa ttttcaatct
    49801 cgacaaacca gttatcgcag ctgtgaacgg ctacgccttt ggcggcggtt tcgaactggc
    49861 gctggcggca gattttattg tttgtgccga taacgccagc ttcgccctgc cggaagccaa
    49921 actgggcatc gttcctgaca gcggcggtgt gctgcgtctg ccgaagatcc tgccgcctac
    49981 catcgtcaat gaaatggtga tgaccggcag acgaatgggc gcagaagagg cgctgcgttg
    50041 ggggatagtc aaccgcgtgg ttagccaggc ggaactgatg gataacgccc gcgaactggc
    50101 tcagcagctg gttaacagcg ccccgctggc gattgcggcg ctgaaagaga tctaccgcac
    50161 caccagcgaa atgccggtag aagaagcgta tcgctatatt cgcagcggcg tgttgaaaca
    50221 ctatccatcg gttctgcatt cggaagatgc cattgaaggg ccgctggcgt ttgccgagaa
    50281 gcgcgatccg gtgtggaaag gacgttaaca accgtgagct attacgcctt tgaggggtta
    50341 attccggtgg ttcacccgac ggcgtttgtc catcccagtg ccgtcttgat tggcgatgtg
    50401 attgtgggag ccggtgtcta catcggccca ctcgcctcac tgcgtggtga ctacgggcgg
    50461 ttgatcgtgc aagcgggagc caatattcag gatggctgca ttatgcatgg ctactgcgac
    50521 actgacacca tcgttgggga aaacggccat atcgggcatg gagcgatcct gcatggttgt
    50581 gtgattggtc gcgatgcatt ggtcgggatg aacagcgtaa ttatggatgg cgcggtcatt
    50641 ggcgaagaga gcattgttgc cgcaatgagc tttgtcaaag caggctttag tggcgagaaa
    50701 cgccagttgt tgatgggtac gccagcccgc gctgtacgca gtgttagtga cgacgagtta
    50761 cactggaaac ggttgaatac caaagagtat caggatcttg tcgggcgctg tcatgcatcg
    50821 ttacatgaaa cgcagccact gaggcaaatg gaggaaaatc gcccccgttt gcaggggacg
    50881 acggatgtga cgcctaaacg gtgatcttgc cggatgcgac ctggttgctg gataagatgc
    50941 tacaacatcg tatctggcaa gcaggacgcc cggtatcaac gagataaaat taacgacgca
    51001 tactctttga caacatcatc tgccacttct gctcgcggtt aagttccgtg agcggaacgg
    51061 caggaatacg cggtgtcttt cttacgccga ctaatttttc ccgactgtta ttgcgcaggc
    51121 gcgcgtaaat ttgctcatcg atatcgacca ctttaaccag tcgctgacac tggcatcccc
    51181 gcccttccag cttattaggg atcatcttaa cttcgcagct tatttctgtg acttccgaca
    51241 gaatataagt cagggtatta accgccttgc tgtgttcaat atcgaaatga atagagatct
    51301 ctcttgctat cacccaccga ttttcagcca tcatccattc ggcgattaac aagtagagtg
    51361 gtttttcaac atatccttca cacatacgaa ctccagtttc acaaattaaa agtctggcta
    51421 tgactcgttt tgaagcaatt aaacaaggcc atattaaaat tgtggatata tcgatagtat
    51481 gcaacttcac cgtcgataag tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt
    51541 ccccaaaaga tttacttaat gggcaaaaaa aaacggtcct catcagagaa ccgtattagt
    51601 tattcgatca aattagcgga tgaaaaatat ccgccacgac acgctattat ttgatctgtg
    51661 cgtgcatttc ctgcaccgaa attacttttt cagtcgcatc ggcattcagc gccatcgcgg
    51721 tagcgaaacc accgttcagg gtggtgtcat aatgcacttt atattgcagc gcactgcgac
    51781 ggatcacgcg ggagtcttca atcgcacgac ggcctgaggt ggtgttgatg atgtaggtat
    51841 attcgccatt cttgatacgg tcctgaatgt gcggacggcc ttcatgcacc ttgtttacca
    51901 gacgcggatt gatacccgct tcgcccagca caatcgccgt gccgtgggtc gcatccagct
    51961 cgaagccctg tttcagcagt tttgccgcca ggtccaccac gcgttcttta tcgccttcgc
    52021 gcacggaaag cagcgcacga ccgtgtttct tcatggtgga gttgctgccc agctgcgctt
    52081 tggcgaacgc ttcagcgaag gtgcggccca cgcccatgac ttccccggta gagcgcattt
    52141 ctggccctaa cagcgggtca acgcctggga atttattgaa cggcagcacc acttctttca
    52201 ccgagtagta cggcggaata acttctttgg ttacgccctg ctcagccagc gatttgccag
    52261 ccatcacacg cgccgccact tttgccagcg gtacgccggt ggctttggag acgaacggaa
    52321 cagtacgcgc cgcacgcggg tttacttcaa tcaggtaaac ttcgttgttt ttcaccgcaa
    52381 actgcacgtt catcagaccg cgcacctgca attcgaaggc cagtttctgc acctgctggc
    52441 gcatcacatc ctgaatttcc tgacttaagg tgtacgctgg cagcgaacat gcggagtcac
    52501 cggagtgcac acccgcctgc tcgatgtgct ccatgatgcc gccaatcagc accatttcgc
    52561 cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc aaggaaatga tccagcaaca
    52621 ctggcgcatc gttagacacg ctaaccgccg tctggaagta gcgacgcagg tcagcttcgt
    52681 catagacgat ttccatcgcc cgaccgccga gaacgtaaga cggacgcacc accagcgggt
    52741 agccaatctc ttttgccttc tcaactgcca tttcaatagc agtaacggtg gcgttcgccg
    52801 gttgtttcag tttcagacgg tcaaccgcat gctggaagcg ttcacggtct tccgcacggt
    52861 cgatagcatc cgggctggtg ccgataaccg gtacgccagc ggcttccagt gcacgcgcca
    52921 gtttcagcgg ggtctgaccg ccgtactgaa cgataacgcc tttcggcttc tcaatacgca
    52981 cgatttccag cacatcttcc agagttaccg gctcgaagta gaggcgatcg gaagtgtcgt
    53041 agtcggtaga gacggtttcc gggttacagt taaccataat ggtttcgtaa ccgtcttcac
    53101 gcagcgccag cgaggcgtgt acgcagcagt agtcgaattc gataccctga ccgatacggt
    53161 ttggaccgcc gccaagcacc atgatttttt cacggtcggt agacggattc gcttcgcact
    53221 cttcttcata agtggagtac atgtaagcgg tgtcggtggc aaactctgcc gcacaggtat
    53281 ccacgcgctt gtagaccggg tgcaggtcat attggtcacg cagcttacgg atttccgctt
    53341 cgcgtacgcc cgccagtttt gccaagcgcg catcggcaaa gcctttgcgt ttcagctggc
    53401 gcaggaattc agcgttcagg ccagtgatgc ccacttccgc cactttctct tccagacgca
    53461 ccagttcttc aatctgtacc aggaaccagc ggtcaatgtt agtcaggttg aagacgccgt
    53521 ccacagacag gcccgcgcgg aaagcatcgg cgatgtacca gatacgctct gcgcctgcgt
    53581 ctttcagttc gcgacggatt ttggttaacg cttccgggtc atccaggctc actttcgggt
    53641 cgaagccagt tgcgcccact tccaggccgc gcagcgcttt ttgcagggat tcctgctgcg
    53701 tgcgaccaat cgccatcact tcgccaaccg atttcatctg agtggtcaga cggtcgttag
    53761 caccggcgaa tttttcgaag ttgaagcgag gaattttagt aaccacatag tcgatggacg
    53821 gctcgaagga ggccggagta cgtccgccag tgatgtcgtt catcagttcg tctagggtgt
    53881 aacccaccgc cagtttcgcc gccactttag caatcgggaa accggtcgct ttcgacgcca
    53941 gcgccgaaga acgggacacg cgtgggttca tttcgataac aatcaggcga ccgtttttcg
    54001 ggttcaccgc aaactggaca ttggaaccac cggtttcaac gccgatttca cgcagcaccg
    54061 ccatcgaggc gttacgcatg atttgatatt ctttgtcggt cagcgtttgg gctggcgcga
    54121 cagtgatgga gtcaccggtg tggatgccca tcgcatcaaa gttttcgata gagcagacga
    54181 tgatgcagtt gtcgttttta tcacgcacca cttccatctc gtactctttc cagccgatca
    54241 gcgactcatc aatcagcaac tctttggtcg gagagagagc cagaccgcgg gcgcaaattt
    54301 cttcaaactc ttcacggtta taagcgatac cgccgccgct accgcccatg gtaaaggatg
    54361 ggcgaataat gcacgggaag cccacgtcag cggcaacagc cagcgcttct tccatcgtgt
    54421 gtgcgatacc ggaacgcgcg gtttccagac caattttctt catcgctacg tcgaaacgac
    54481 ggcggtcttc tgctttatca atcgcatcgg cagtggcacc aatcatggtg acaccgaact
    54541 cttccaacac gccctgacgt tccagctcca gcgcgcagtt cagcgccgtc tgaccgccca
    54601 tggttggcag caccgcgtcc gggcgctctt tttcaataat cttgcgtacc acttcccagt
    54661 gaatcggctc gatgtaggtc gcatcggcca tttccgggtc ggtcatgatg gtcgccgggt
    54721 tggagttcac cagaatgacg cggtaaccct cttcgcgcag ggctttacat gcttgcgcgc
    54781 cagagtagtc aaactcacac gcctgaccga taacaatcgg gcccgcaccc agaatcagga
    54841 tactttttat atctgtacgt tttggcatgg ctcttttact cctgattact tagcggtttt
    54901 acggtactgc tcaattaact tgataaagtg gtcgaacaac ggcgcggcgt cgtgtggacc
    54961 agggctggct tcagggtgac cctggaagct gaacgccggc ttatcgatgc gatgaatgcc
    55021 ctgtaacgta ccgtcgaaca gggatttatg cgtgacacgc aggtttgcag gtaatgttgc
    55081 ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc accacgtttt tctccacatc
    55141 tttaaccgga tggttgccgc cgtggtgacc aaatttcatt ttgacagtct tcgcaccgct
    55201 cgccagcgcc agcagctgat gaccgagaca gatgccgaat accggaatat cggtttcgag
    55261 gaacttctgg atggcggtaa tggcgtaatc gcacggggcc gggtcgccag gaccgttgga
    55321 gaggaagatg ccgtctggat tcattttcag cacatcttcc gcagaagttt gcgccggaac
    55381 gatggtcagg cgacagcctc tatccaccag catccgcagg atgttgcgct tggcaccaaa
    55441 atcataagcc acgacgtgga acggcagctc gtcttctttt ttcgcttctg gcaggccgcc
    55501 ggtcaacgtc cagctccctt gtgtccagct ataggcttct gcggtggtca cttcttttgc
    55561 cagatccatg ccgttcagac ctgggaacgc gcgggctttt tctaacgcca gcgccgcatc
    55621 cgggttatcg cctgcgataa tgcagccatt ctgtgcgcct ttctcgcgca gtaaacgcgt
    55681 cagcttacgg gtatcgatat cggcaatcgc cacgatgtta tggcgcttca ggtaagaaga
    55741 gaggtcttcg gtattacgga agttgctggc aatcagcggc aggtcgcgaa tcaccagacc
    55801 ttgtgcatgt acctgagaag attcttcatc ggcgtcatta gtgccgacat tgccaatatg
    55861 gggataagta agagtaacga tttgacgaga ataggaagga tcagtgagga tttcttgata
    55921 accggtcatt gaagtattga aaacgacttc cccaaccgcc gaacctgttg cccctatggc
    55981 ccgaccgtga aactgggttc cgtcttccag aaccaatagc gctgacttaa tcaaaacacc
    56041 ctccagagaa tactcactca ctttatttgc atattaattc acagtgatga cgtaaatcaa
    56101 tgcaaatctg cttaccgacg aatttctggc aaacggcggc attctggaga tatgggcgct
    56161 aaaagtcaac ttaatggcag gattttttat attattttgt ggctcatgca tgttttacag
    56221 gcattaagcg acaaaaacat catctaacct gttctggaaa acgcttgcgc agcaagaaga
    56281 ttgcatgaaa taaaaacgag caggaaaaag tggaccaaat ggtcaaaatt tacattaaaa
    56341 caacaaaaat agaagccgaa acatgtaatt tttggcagtt atagaaaatt taaaagcata
    56401 acaccaaaaa taaaagggct aaaaattaaa gccctttata attaataaat taaatgtgtt
    56461 acttttgcat cataataaac attttgtggt tacaaattat tgagatcaag cacatctcgc
    56521 atatcaaaaa gaccactttc cttaccactc aaccacaaag ccgatcttac cgcaccgtta
    56581 gcaaatgtca tgcggctgga cgccttatgg gtgatctcca gacgctcgcc aatatcggca
    56641 aacatcgcgg tatgttcgcc aacgatgtca cctgcacgca cggtagcaaa accaatggta
    56701 cctggcacac gttcaccggt atggccttca cgactgtaga ccgcgcaatc tttcagatct
    56761 ttatcaaggg cgtgggcgat cgcctctccc attgccagtg cggtgcctga cggcgcatca
    56821 actttatgtc tatgatgtgc ttcaataatt tcgatatcgg tgtagtcacc catcactttg
    56881 gctgctttct ccagcagctt aagcatgacg ttaacgccaa cgctaaaatt ggcggcaaag
    56941 acaatcgcaa tatcggcggc ggcgtcacga attgcctgtt taccggcttc gtcaaacccc
    57001 gtagtgccga tcaccatccc tttgccatgc tgccgacaaa aagcgagatg gttcagcgta
    57061 ccttccggac gggtaaaatc gataaacaca tcaaaatcat cttttatcgc atcaagactg
    57121 ctttgcacgg taacgcctgt tttccctgca ccagccagct caccggcgtc gctgcccagt
    57181 aaagaagatc cttcgcgctc cagcgccgcg cccagctgca cgccttctaa tgccagtgcc
    57241 gcctgaatca actggcggcc catacgcccc ccggctcccg cgatggcaac gcggatgttt
    57301 gcatcatgca tagctattct cttttgttaa tttgcataga ccgttttcag agtaaccagc
    57361 gcatcgctac gccgccagcc aaaaacgctg ataattagaa attaatgata aacagggagg
    57421 gatatcagtg aaaggcatga gtcgatgaat gactgcatgc cgataacatg tgacaggtta
    57481 cggcaccaga gccagcacct cagccaccca ctgttggaag ccttttacat tcagatccaa
    57541 tgccacctgt acattggctg gtttgcccag gcaaccgtcg atatcaacca ccgtcgtgcc
    57601 tgaggtaaat tcgccttgag tttctaccgc gacaaaacac ggcttgaggg tgaataagtc
    57661 cgggcgcacc agccaggcga tggcgcagag atcgtgcatt cgcagaccgc tttgcatact
    57721 gccgctacgg tagtggctaa acagggcgtg gagcattttt ccggtgcgat tcaactccgg
    57781 taaagtagcg agatagtcag gggttaatat tgcctgattg gtgacatcca gaccgcacat
    57841 gacaatttcg atgccgctac ggaagacaca ggcagccgct tctggatcgg cagcgatatt
    57901 aaactcggca tttggtgtac agttgccgcg accagcagaa ccgcccatga tcaccagacg
    57961 acgaatatac ggtttacatt ccgggcattg taaaagtaac agcgcaatat tggttaacgg
    58021 gccgatggcc accagggtaa cgggctctgg tgcacgcatc agggcatccc gaatcgccag
    58081 aaacgccggt atcccgagtg gctttcggtt gtgctcaaca aagtcgtagc cagccattcc
    58141 cgattcgccg tgcacagatg ctgcatcacg cggtgcgcgt accagcggca cagcggctcc
    58201 ctgggcgagc ggaatctccg cattccagaa atgcagcagt tgcagggcat tgcgggtagt
    58261 tttctcaacc gagacattac ccgcgacggt ggtcatcagt tgcaggtcga gttcgggggc
    58321 aaaaatcgcg gcggcaatgg cgacggcatc gtcaatgccg gggtcagtat cgaggaagat
    58381 aggtaaacgc atgttttctt cataaaaaat gccggtaaca agaccggcat tttcgcataa
    58441 cttaggctgc taatgactta atcgacttca cgaatatcga cacgcagctc tttcggcact
    58501 tcgaaaacaa tattttcttc acggccttcc agcggaatgg cttcaccacc acccaactgc
    58561 tgcaaacgtg ccaccacatt ctgcaccaga atatccggag ccgatgcgcc cgcagtcacg
    58621 ccgacgcatt taacctcttt cacccactct tcctggatat ctttcgcatc gtcaatcaaa
    58681 aacgcgtgtt tgcccatacg ctgggccagc tccgccagac ggttggagtt ggaggagttt
    58741 ttcgaaccga ccaccaacac aacttccgcc tgttctgcca gggcgcgtac cgcttcctga
    58801 cggttagtcg tggcgtagca gatgtcatct ttgcgcggac cgacaatttt cgggaagcgt
    58861 ttacgcagcg cgtcgatcac atcagacgtg tcatccaccg acagcgtggt ttgggtcata
    58921 aaggagagct tctcttcgtt tttgaccgtc agtttccaca catcgtctgg cgattcgacc
    58981 agatacattc ccccttcggg gttgctgtac tgccccatcg tcccttccac ttccgggtgc
    59041 ccggcgtggc cgatgagaat agattcttcg ccacggcgac tggcgcgggc aacttccata
    59101 tgcactttgg tcaccagcgg acaggtggca tcaaacaccg tcaaatcgcg gcttttcgct
    59161 tcgttacgaa ccgcctgaga aacaccgtgt gcggagaaaa tcaggatcgc gccgtccggc
    59221 acttcgctaa tctgctcaat aaagatagcc ccacgctcgc gcaagctatc gaccacgtag
    59281 cggttatgca ccacttcgtg acggacatat atcggtgcgc cgtaaatggc cagcgcgttt
    59341 tcaacaatgc tgatagcgcg gtctaccccg gcacaaaaac cacgcgggtt ggccaacagg
    59401 atctgcatgt tacgcctcca gtgccggatc gatttccagc acttcaatat caaaatgaac
    59461 ggtctgcccg gccagcggat ggttgaaatc aacggtaatg gagtcgccgt taatttcgcg
    59521 gatcacacca ggcatctcac tgccatccat tgcggtaaaa agcatgattg cgccaatttc
    59581 tggctcgcct gcatccataa attcacggcg ggagaagtac tgaatcaggt ccggtgacgg
    59641 cacgccaaac gccgcatctg gctccagcga gaaggtggtt ttgtcgccca ctttcagccc
    59701 cagcaggtgt tgctccagcc cttcagaaag agaagcatcg cccaggcgga acagcgccgg
    59761 tttaccgttg ttgcgagtag actcggcggt ggtgccatcg tcgagtttta gcgtgaagtg
    59821 caccaggacg gcgctattgc tctgtacaga ttcagacatg caggtttgct cttatttttg
    59881 aggatttttg ccggatgcgg cctacgattt cgtagcaatc tgtaggccgc atccggcagg
    59941 gtttattatt gttttttcgc tttggaaggt aaaaaccctt ccagcacgat cagtgccgca
    60001 ccgacacaaa tggcagtatc ggcaaggttg aaggtggcga agtgccagtc gccgacgtag
    60061 aagtcgatca tatcgacaac gaagccgtgc cacaggcggt cgaacaggtt gcccagcgcg
    60121 ccgccaataa tcagcgcgta agcgatattg tttagcttct gcgtggcctt cgagcgatac
    60181 atcatcactg ccaggagcac gctaatacca atcgcaatac cggcaaagaa ccaacgctgc
    60241 cagccgccgc tatcggcaag gaaactaaac gccgcgccat agttacgcgc ataatgcaga
    60301 ttaagcgacg ggaacagcgg gaccgtatcc cccagagcaa agttctggag gatcaggtat
    60361 ttgctgccca gatcgataat cagcacgact accaccagcc acagccagcg tagccctgtt
    60421 gaacagatcg attgactcat caggcaaact tacgtttttc accgtcaccg gcgacgttgc
    60481 tgacacagcg gccgcagatt tctgcgtgtt ccgccacctt gccgacatcc tgggtgtagt
    60541 gccagcagcg tgggcacttc tcaccttcgg ctttactcaa cgcgactttc agccctttga
    60601 gtacttcgct ctgctgagca tcagcaggtg cgtcgttata gtctgcaacg gtagcgccgg
    60661 aggtcaacag gacaaatcgt aattcatcgc ccagcgcggt cagtttcgcc gccagttccg
    60721 gttctgcata caaggttact gccgcttcca gcgagccacc cactttcttg tcggcacgcg
    60781 cttgctcaat gactttgttc acttcgccac gcactttcaa cagctcgtcc cagaacgcat
    60841 cgttcattgc ttcactgtct gccagaccaa acaggccttc gtaccactca ccggtgaaga
    60901 cgtatttttc acgttcgccc ggcagatagc cccacacttc atcagcggtg aaggagagga
    60961 ttggtgccat ccagcgcacc agcgcttcag cgatgtgata cagcgcagtc tggcagctac
    61021 gacgcgccac gctatccgct ttggcggtgt actgacggtc tttgatgatg tcgaggtaga
    61081 aggaacccat ctcaacggag cagaagcgca tcagacgctg taccacttcg tggaaatcgt
    61141 atgcttcgta cgccttgagg atgtcttcct gtgccgcttt cgcacaacct acggcccagc
    61201 gatccagtac caccatctct tccggtttca ccatatcttt tgctggatca aaaccattca
    61261 ggtttgccag caggaagcgc gcggtgttac ggatacgacg atagctatcg gcagcacgtt
    61321 tcaggatctc gtcagaaacg gccatttcac cggtgtagtc ggttgatgcc acccacagac
    61381 gcagaatatc cgcgcccagt ttgttcatca catcctgcgg cgaaacggtg ttgccgatgg
    61441 atttagacat cttgcgaccc tgaccatcca cggtaaagcc gtgggtcagt acctgacgat
    61501 acggcgcttt gcctttcatc gcggtggaga tcattaggga agacatgaac caaccacggt
    61561 gctggtcaga accttccaga tacatgtccg ctgcgtgacc ggcaaattcc ggacgcacgt
    61621 caacaacaga agagtgggta gatccggagt caaaccatac gtcgagagta tccggcactt
    61681 tcacatactg gtcagcttcg tcgccgagga tctctttcgc atcgagatcc caccacgcct
    61741 ggatgccgtc aacttcaacg cgtttagcca cttcttccat cagttcgagg gtacgcggat
    61801 gcagctcttc cgtgtctttg tgcacgaaca gtgacatcgg tacgccccag gtgcgctgac
    61861 gggagataca ccagtcagga cggttagcaa ccatcgactc gatacgcgcc tggccccagt
    61921 ccgggagcca ctgcacgcct ttgatctctt tcagtgactg cgcacgcaga cctttctgat
    61981 ccatgctgac gaaccactgc ggtgtcgcgc ggaagatgat cggcgttttg tgacgccagc
    62041 agcacggata gctgtgctgc attttctcaa cgtgcagcag cgcgcctttt tcctgcagca
    62101 gcgcaacgac gatgtcgttc gctttgaaga cgttcacgcc atccagcgtc ggataagtgc
    62161 ccggcagata agtgccgtcc gggccaaccg ggttagcggt ttccaggccg tatttctgac
    62221 cgatcacata gtcgtccggg ccgtggccag gcgcggtgtg aacggcaccg gtaccggcat
    62281 ccagggtaac gtgatcgccg aggattgccg gaacgtcgaa gcccataaac ggatgggtaa
    62341 agcgcagcag ctcaagctct gcacctttta ccgtgccgag aatggtgtaa tcggtcacgc
    62401 cgatacgctg cattacgctt tcaaccagat ctttcgccag aatcacggcc tgaccgtcga
    62461 tctgcaccag cgcatagtcg aaatcaggtg caatagagat tgcgcggttc gcaggcagag
    62521 tccacggcgt agtggtccag atcaccagcg agattgggcc gttaacgttg cttacgccaa
    62581 atttcgcttt cagcgcatcc tgatcgaccg cctggaaagc gacgtcaatg gacggagaag
    62641 ttttgtcgta atactcaact tccgcttctg ccagtgcaga acggcagtct acgcaccagt
    62701 gcaccggctt cgcgcctttg tgcaggtgac cattgccgat gattttgccc agcgcgcgga
    62761 tgatgttggc ttcagttttg aagtccatag tcagatacgg gtgcgaccag tcgcccagca
    62821 cgcccagacg gataaagtct ttgcgttgac cgtcaacctg ggtcgccgcg tattcgcggc
    62881 acttggcgcg gaactcggcg gcggtgaatt tctcacccgg cttaccgtat tcttgctcta
    62941 ctttcagctc gatcggcaga ccgtggcagt cccagccagg cacatacggc gagtcataac
    63001 cggaaagccc tttggacttc acgataatgt ctttcagaat cttgttaacc gagtgaccaa
    63061 tatgaatgct gccattcgca taaggagggc catcatgcag aatgaaggtt tttttgcctt
    63121 ttttagccgc acggatgatg ccgtacagat catcatcagt ccaacgcgcc agcattcccg
    63181 gttcgcgctt ggcgagatcg ccacgcatcg ggaaccctgt ttccggcaaa ttcagggttg
    63241 atttatagtc actcatcaga ttctcggttc cgtgtttcgg tttgattaca taacaggctt
    63301 aagccggttt tgttagccca aaaaattcgc gggcggttaa ttcatcacgc gcaatctgcg
    63361 ctttcagttc gtccagcgac gcaaatcgct gctcattgcg tattttttta cgcagcacta
    63421 cttgtatatg gcgaccgtaa aggtccattg caacatctaa caaatgcact tccagctgct
    63481 ggcgaatacc ggcaaccgtt gggcgtgttc cgatgtttgc cacgccgggt aacggctttt
    63541 caccgaggcc cagcacttct accgcataaa cccctttcac cggggaaacc tgacggcgta
    63601 gcggtacatt cgccgtcggg aaacctatag tgcgccctaa ttcatcaccg tggactacac
    63661 gcccggagat ggcaaacggg tgccccagta aactttctgc cagagccaga ttgtcatcag
    63721 ccagcgcctg acgcacggcg gtgctgctga tacgcacacc accttcgcaa aaagtttgcg
    63781 tactggtgat atcgaagccg tattccatgc cagctttctg taataacaag aaatcgcctt
    63841 cacgaccagc gccaaagcgg aaatcatcac ctacggcaag aaattttacg cgcaaatgct
    63901 tcaccaaaag atcgctgatg aaattttgcg cggttaacgc cgcaaaacgc ctgtcgaaac
    63961 gcacgcacag cacgtaatca acgccacact ctgcaaggta acgcagtttt tcccgcagcc
    64021 gggtcagtct tgccggggct ttatcggtag caaacagttc cagtggttga ggttcaaaaa
    64081 gcatcaccat caccggtaag ttgcgcttgc gcccttcttc ctgcaagccc tgtaacagcg
    64141 cgcgatgacc gcgatgcacg ccgtcgaaat taccaatagt cagcacacac ccttcttgcg
    64201 gggcctggct gagattatgt atgccgcgta tcagcttcat gtctggctca aaacagtgaa
    64261 aatcgtccga gtataccttg tacagcggtc aaggttaacc ggcgattgag taccgaaatc
    64321 agaaagaagt ggcttttcat tgccatggag caaatcacgg gaagaaactg accgcctgct
    64381 gcaattttta tcgcggaaaa gctgtattca caccccgcaa gctggtagaa tcctgcgcca
    64441 tcactacgta acgagtgccg gcacattaac ggcgcttatt tgcacaaatc cattgacaaa
    64501 agaaggctaa aagggcatat tcctcggcct ttgaattgtc catatagaac acatttggga
    64561 gttggacctt ggctaatatc aaatcagcta agaagcgcgc cattcagtct gaaaaggctc
    64621 gtaagcacaa cgcaagccgt cgctctatga tgcgtacttt catcaagaaa gtatacgcag
    64681 ctatcgaagc tggcgacaaa gctgctgcac agaaagcatt taacgaaatg caaccgatcg
    64741 tggaccgtca ggctgctaaa ggtctgatcc acaaaaacaa agctgcacgt cataaggcta
    64801 acctgactgc acagatcaac aaactggctt aatcgccaat ttgctgaagc tttgtgaaaa
    64861 agcccgcgca agcgggtttt tttatgcctg ctgcttttgt gatgcgtgag agaacacact
    64921 gccggttgcc agatagtcaa aagagacatg ccgttgtact atctgaactc ttcgtggctt
    64981 aagaatcgta gaaccatcgg ctgatacatt ctgaacaaga atatctactc aatctccaga
    65041 ctcccccaca aagaatatgg atattgtgat acacattgag gtattaaact taagaccact
    65101 ctatttttga attaaattcc tatatacttt aaccattcat aaatatctcc tcattagacc
    65161 t
//
LOCUS       DAJTRB010000010        63376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTRB010000010 DAJTRB010000000
VERSION     DAJTRB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.010"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO330_000701"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO330_000701"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5881686.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO330_000702"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO330_000702"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5881687.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO330_000703"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO330_000703"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5881688.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO330_000704"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO330_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5881689.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPV
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO330_000705"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO330_000705"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5881690.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQEWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO330_000706"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO330_000706"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5881691.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO330_000707"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO330_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5881692.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO330_000708"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO330_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5881693.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO330_000709"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO330_000709"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5881694.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO330_000710"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO330_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5881695.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO330_000711"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO330_000711"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5881696.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO330_000712"
     CDS             complement(15665..16702)
                     /locus_tag="OO330_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5881697.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO330_000713"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO330_000713"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5881698.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO330_000714"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OO330_000714"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5881699.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OO330_000715"
     CDS             19444..19884
                     /locus_tag="OO330_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5881700.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO330_000716"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OO330_000716"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5881701.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OO330_000717"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OO330_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO330_000718"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OO330_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5881702.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO330_000719"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OO330_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5881703.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO330_000720"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OO330_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5881704.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO330_000721"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OO330_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5881705.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO330_000722"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OO330_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5881706.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO330_000723"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OO330_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5881707.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO330_000724"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OO330_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5881708.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO330_000725"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OO330_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5881709.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="OO330_000726"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="OO330_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5881710.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO330_000727"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OO330_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5881711.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO330_000728"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OO330_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5881712.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO330_000729"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OO330_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5881713.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQVA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO330_000730"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OO330_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5881714.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO330_000731"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OO330_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5881715.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="OO330_000732"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="OO330_000732"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5881716.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="OO330_000733"
     CDS             37412..37681
                     /locus_tag="OO330_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5881717.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="OO330_000734"
     CDS             complement(37684..38043)
                     /locus_tag="OO330_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5881718.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="OO330_000735"
     CDS             38184..38810
                     /locus_tag="OO330_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5881719.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="OO330_000736"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="OO330_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5881720.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSPPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO330_000737"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OO330_000737"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5881721.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41649..42314
                     /locus_tag="OO330_000738"
     CDS             41649..42314
                     /locus_tag="OO330_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5881722.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42387..42944
                     /gene="dcrB"
                     /locus_tag="OO330_000739"
     CDS             42387..42944
                     /gene="dcrB"
                     /locus_tag="OO330_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5881723.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42948..44165)
                     /locus_tag="OO330_000740"
     CDS             complement(42948..44165)
                     /locus_tag="OO330_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5881724.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44299..45348
                     /locus_tag="OO330_000741"
     CDS             44299..45348
                     /locus_tag="OO330_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5881725.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45403..45990
                     /gene="acpT"
                     /locus_tag="OO330_000742"
     CDS             45403..45990
                     /gene="acpT"
                     /locus_tag="OO330_000742"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5881726.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46101..47675
                     /gene="nikA"
                     /locus_tag="OO330_000743"
     CDS             46101..47675
                     /gene="nikA"
                     /locus_tag="OO330_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5881727.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVPATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47675..48619
                     /gene="nikB"
                     /locus_tag="OO330_000744"
     CDS             47675..48619
                     /gene="nikB"
                     /locus_tag="OO330_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5881728.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48616..49449
                     /gene="nikC"
                     /locus_tag="OO330_000745"
     CDS             48616..49449
                     /gene="nikC"
                     /locus_tag="OO330_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5881729.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49449..50213
                     /gene="nikD"
                     /locus_tag="OO330_000746"
     CDS             49449..50213
                     /gene="nikD"
                     /locus_tag="OO330_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5881730.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50210..51016
                     /gene="nikE"
                     /locus_tag="OO330_000747"
     CDS             50210..51016
                     /gene="nikE"
                     /locus_tag="OO330_000747"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5881731.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51022..51423
                     /gene="nikR"
                     /locus_tag="OO330_000748"
     CDS             51022..51423
                     /gene="nikR"
                     /locus_tag="OO330_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5881732.1"
                     /translation="MQRVTITLDDDLLKTLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51543..51902)
                     /locus_tag="OO330_000749"
     CDS             complement(51543..51902)
                     /locus_tag="OO330_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5881733.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYVRTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51899..52174)
                     /locus_tag="OO330_000750"
     CDS             complement(51899..52174)
                     /locus_tag="OO330_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5881734.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52233..53357)
                     /locus_tag="OO330_000751"
     CDS             complement(52233..53357)
                     /locus_tag="OO330_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5881735.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53357..56092)
                     /gene="rbbA"
                     /locus_tag="OO330_000752"
     CDS             complement(53357..56092)
                     /gene="rbbA"
                     /locus_tag="OO330_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5881736.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56089..57156)
                     /locus_tag="OO330_000753"
     CDS             complement(56089..57156)
                     /locus_tag="OO330_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5881737.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57522..59144)
                     /locus_tag="OO330_000754"
     CDS             complement(57522..59144)
                     /locus_tag="OO330_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5881738.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59406..60995)
                     /locus_tag="OO330_000755"
     CDS             complement(59406..60995)
                     /locus_tag="OO330_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5881739.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61379..62410
                     /locus_tag="OO330_000756"
                     /pseudo
     CDS             61379..62410
                     /locus_tag="OO330_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62497..62973)
                     /locus_tag="OO330_000757"
     CDS             complement(62497..62973)
                     /locus_tag="OO330_000757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881740.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62966..>63376)
                     /locus_tag="OO330_000758"
     CDS             complement(62966..>63376)
                     /locus_tag="OO330_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5881741.1"
                     /translation="SHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKDTY
                     SITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDVNS
                     MHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT        15208 a        17168 c        15765 g        15235 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccaacag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccattcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcagatga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtaccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttatgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccacctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagcccgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa acggcgctag
    41461 tttacgccct gtgagtccgt aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg
    41521 cattgtcaac gtaaagcagt atcatgcggc ggctcgaaaa aagggtaagc acgttattat
    41581 gttaaggtaa cagacgtgtc gtacgtattg ggttccctca ccccaatggt taatcaaaaa
    41641 ggtacaatat gaacgttttc tcgcaaactc aacgctataa ggcgttgttc tggttatcgt
    41701 tatttcatct tctggtgatc acctccagta actatctggt tcagcttccc gtctccattt
    41761 ttggtttcca taccacctgg ggcgcgttta gctttccgtt tatttttctt gctaccgacc
    41821 tgaccgtgcg tatttttggc gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc
    41881 ctgcgttatt aatctcctac gtcatctcgt cgctattcta tatgggttcc tggcagggat
    41941 tcggcgcact cgcccacttc aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg
    42001 cctacgcgct ggggcaaatc ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc
    42061 gctggtggct ggcaccgaca gcgtccacac tgttcggtaa cgtcagcgac acgctggcct
    42121 ttttcttcat tgccttctgg cgtagcccgg atgcctttat ggctgaacac tggatggaaa
    42181 tcgcgctggt cgattactgt ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt
    42241 atggcgtatt actcaatatg ctgttgaaaa gactggcaga taaatccgaa atcaacgctt
    42301 tgcaggcgag ttaaaggttc gttatcagag ttgtgataag atggatgaat gagccgttat
    42361 ggccgtttat cgaaaggaag aagtcaatgc gcaatctggt taaatatgtc ggaattggcc
    42421 tgctggttat ggggcttgcg gcctgtgatg ataaagacac taacgctacg gcgcagggtt
    42481 cggttgcgga aagtaacgct accgggaatc ccgtcaacct gcttgatggc aagttaagtt
    42541 tctcgctgcc agcggatatg accgaccaga gcggtaagct gggaacgcag gccaataaca
    42601 tgcatgtctg gtccgacgcc accgggcaga aagcagtcat cgtcatcatg ggcgatgatc
    42661 cgaaagaaga tctggcggtg ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc
    42721 cgcagctgca agtggtaacc aataaagcca ttgagctgaa aggtcacaaa atgcagcagt
    42781 tagacagtat tatctccgcg aaaggccaga cggcgtactc ttccgttatt ctgggtaacg
    42841 tgggtaatca actgctgacc atgcaaatta cgctgccagc tgacgatcag caaaaagcgc
    42901 aaaccaccgc agaaaacatc attaatacgc tggttattca gtaagtttta agatgatgag
    42961 gcggcctcag ggacgtgttc cggaggccgt ttttttaatc gccacgtcag taataacgcg
    43021 attgcgacca gtcccgccgc cgccagataa atcaccggta cgcccgccca gctcatcacc
    43081 agcccagcca gtggtccagt cacgccaagc gataaatcca taaatacggt gtaagttgcc
    43141 agcgccgccc cctgattttg ctgcggaacc gcttttaccg ccactacacc caatgccggg
    43201 aacaccagcg aaaacccggc ccccgccagt aagacgccga ttttcgccat ccacggcata
    43261 gtcgccacgc caaccagtag caggccgatt atctcaacgc taaagcaaat catcgctacg
    43321 tttaagccac cgatacggtt aatgccgtta gggaataaca aacgcgtacc gacaaacgca
    43381 cagctaaaca gcgtcagcgc gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc
    43441 gtgataaagg tggcgatgac gccaaatccg gcggaagcca gtgccagcgc cataccgtac
    43501 agccagacgc gcccaagcac cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc
    43561 gtcggacgcg ggatcgccaa caaaatggcc accagcgcca cgcccataat gattaacgct
    43621 aacgcctgca agccgcccca gtgataaaac acgacgccta acggcgcacc catcgccatc
    43681 gccccgtaag tgacaatgcc gttccacgaa atcacccgcc cgatatgcag cgagccaacc
    43741 actccaacgc cccatagggt cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg
    43801 acgcgcccca ggcaaagtaa taacaggctg atgacaggca gactggcggt taatcctgcc
    43861 gtcagatacc ccagaccgct caaaaagcag ccgcataaac cgaagacgac aatctttttg
    43921 ggtcccagca aatcggcgta acgtccggca tgagggcggc tcagcaaggt ggcgaaatat
    43981 tgcaggctga taaccaatcc tgcccagaag gcgctaaagc ccatcacatc atggacatag
    44041 cccggtaata cagcgagcgg caacccgatg gtgaggtagc tggcgaagtt aaacatgaca
    44101 atggagacaa tgcgcaaatt caggcgcaat ccgtttagcg cgggttcggc tacgggttcg
    44161 ggcatgagga tcaccacatt tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa
    44221 aatcagcagt aagaatcaga atattgctgg cgtgactccc gcgctacact taacacaaaa
    44281 agtcacaagg aagccccaat ggaaacccct caacccgata aaacgggcat gcacattctg
    44341 ctcaagttgg cctcgctggt agtgatcctc gcgggcattc acgcagcggc agatatcatt
    44401 gtgcagctgt tactggcgct gttttttgcc atcgtcctca acccgctcgt tacctggttt
    44461 attcgtcggg gagtacaacg ccccgttgcc attacgattg tagtggtggt gatgctgatc
    44521 gcactaaccg cgctggtcgg cgtactggcg gcatcgttta acgaatttat ctctatgctg
    44581 ccgaagttta ataaggagct gacgcgcaaa ctttttaaat tgcaggagat gttgcctttt
    44641 cttaatttgc atatgtcgcc ggagcgaatg ctgcagcgga tggactcgga aaaagtggtt
    44701 accttcacca cagcgctaat gaccgggctt tccggggcaa tggcgagcgt gcttttgctg
    44761 gtgatgaccg tagtttttat gctgtttgaa gtgcgccacg tcccttacaa aatgcgtttt
    44821 gcgctgaata acccacagat tcacatcgca ggattacacc gcgcacttaa aggcgtttcg
    44881 cattatctgg cgttaaagac gctactcagt ttatggacag gtgtcatcgt ctggctgggg
    44941 ctggcgctaa tgggcgtaca gtttgcgctg atgtgggcag tactggcgtt tttgctcaac
    45001 tacgtgccca atatcggcgc ggtaatttcc gccgtaccgc caatgattca ggtgctgctg
    45061 tttaatggtg tttacgaatg tattctggtc ggcgcattgt ttttagtggt ccatatggtc
    45121 atcggcaata ttttagaacc acggatgatg ggccatcgcc tggggatgtc caccatggtg
    45181 gtatttcttt cattgttaat ctggggatgg ctgctcggcc cggtagggat gctactttcg
    45241 gtgccattaa ccagcgtgtg taaaatctgg atggaaacga cgaaaggtgg cagcaaactg
    45301 gcgattttac tggggccggg cagaccgaaa agtcggttac cgggatgagg cgacaagtga
    45361 tacgatacgc actttcattt tccattaaac gttggccctg atatgtatcg gatagttctg
    45421 gggaaagttt cgaccttaag cgcagctcca ctgccaccgg gtttacgcga gcaagcaccg
    45481 caaggtccac gacgcgaacg ctggctggcg gggcgtgcat tgctttcgca cacgctttcc
    45541 ccgctaccgg agatcatcta tggcgaacaa ggcaaacctg cattcgcgcc ggaaacgccg
    45601 ctatggttca acttaagcca tagcggtgac gatatcgctc tgctgttgag cgatgaaggc
    45661 gaagtcggct gcgatatcga agtgattcgc ccgcgcgcca actggcgctg gctggcgaac
    45721 gccgtgttca gcctcgggga acacgctgaa atggacgccg tgcatcctga tcagcaactg
    45781 gaaatgttct ggcgcatctg gacgcgcaaa gaagccatcg ttaaacagcg tggcggcagc
    45841 gcctggcaaa tcgtcagcgt agatagcacc tatcactcct cgctgtcagt cagccattgt
    45901 cagctagaaa atttaagcct tgcgatctgc acccccaccc cctttacgct caccgccgac
    45961 agtgtgcaat ggatcgattc agttaactga tccgcccacc cgactgccca tctattgatc
    46021 cagaacaggt aatcagtatg acgaatactt aaaatcgtca tacttatttc cgccatctat
    46081 tttaatccat tggggttacc atgctctcca cactccgccg cactctattt gcgctgctgg
    46141 cttgtgcgtc ttttatcgtc catgccgctg caccagatga aatcaccacc gcctggccgg
    46201 tgaatgtcgg gccactaaac ccgcaccttt acacgcctaa ccagatgttc gcccagagca
    46261 tggtttatga accattggtg aaatatcagg cagacggttc ggtgatcccg tggctggcaa
    46321 aaagctggac tcattcagaa gatggtaaaa cctggacctt caccctgcgt gatgacgtga
    46381 aattctccaa cggtgaaccg ttcgatgccg aggcggcggc agaaaacttc cgcgcagtgc
    46441 tcgataaccg tcaacgtcac gcctggctgg agctggcaaa ccagattgtt gatgttaaag
    46501 cactcaataa aacagagctg caaattaccc tgaaaagcgc ctactatcct ttcctgcaag
    46561 aactggccct gccccgtcct ttccgtttta tcgctccctc gcagtttaaa aaccatgaaa
    46621 ccatgaacgg aattaaagcg ccgattggca ccggaccgtg gattttgcag gaatcgaaac
    46681 tgaatcagta cgatgtcttc gtccgtaacg aaaactactg gggcgaaaag ccagcgatta
    46741 aaaagatcac ctttaacgtc atcccggacc cgactacccg cgcggtggcg tttgaaactg
    46801 gcgatatcga cctgctgtac ggaaacgaag ggttattacc gctcgatacc ttcgcccgct
    46861 ttagccagaa tccggcttac cacacccaac tgtcacagcc gatcgaaacc gtgatgctgg
    46921 cgctcaatac cgccaaagcc cccaccaacg agctggcagt acgtgaagct cttaattacg
    46981 cggtaaacaa aaaatcgctg attgataacg cgttatatgg cacccagcag gtcgccgaca
    47041 ccctgtttgc cccttctgtg ccttacgcta atctcggcct gaaaccgcgc cagtacgatc
    47101 cgcagaaagc gaaagagttg ctggaaaaag ccggttggac gctgcctgcg ggcaaagaca
    47161 tccgcgagaa aaatggtcag ccgctgcgca ttgaactgtc gttcatcggc accgatgcgt
    47221 taagcaaatc gatggcggaa atcattcagg ctgatatgcg ccagattggc gcagatgtct
    47281 cgctgattgg cgaagaagag agcagtatct atgctcgtca gcgcgacggt cgttttggca
    47341 tgattttcca ccgcacctgg ggcgcgccat acgatccaca cgccttcctg agttcaatgc
    47401 gcgtaccgtc gcacgctgac ttccaggcac agcaaggatt agccgacaag ccgctgattg
    47461 ataaagagat cggcgaagtg ccggcgaccc atgacgaaac gcaacgtcag gcgctgtatc
    47521 gcgacattct gacccgtctg catgacgagg cggtttatct gcctatcagc tacatctcga
    47581 tgatggtggt atcaaaaccg gagctgggta atatccccta cgcgccgatc gccaccgaaa
    47641 ttccgttcga acagattaaa ccggtgaaac cttaatgttg cgttacgtat tacgccgctt
    47701 tctgctgctg atcccgatgg tgctcgccgc ctcggtgatc atttttctga tgctgcgcct
    47761 cggtaccggc gacccggcgc tcgattatct gcgtttatct aacctgccgc cgacgccgga
    47821 gatgctggcc tctacccgca ccatgctcgg gctggatcag ccgctgtacg tccagtacgg
    47881 tacctggttg tggaaggcgc tgcatcttga ctttggtatc tcattcgcca gccaacgccc
    47941 ggtactggac gatatgctga acttcctgcc cgccacgctg gaacttgcag gtgcggcgct
    48001 ggtattaatt ctgctcactt ccgtaccgct cggtatctgg gcggcgcgcc accgcgatcg
    48061 tttgccggat ttcgccgtgc gctttatcgc gtttcttggc gtgtcgatgc ccaacttctg
    48121 gctggcattt ttgctagtga tggcgttttc ggtgtatctg caatggctac ccgcgatggg
    48181 ctacggcagc tggcagcaca tcattttacc tgcggtttcc atcgccttta tgtcgctggc
    48241 gattaacgcg cgactgctgc gcgccagtat gctggacgtc gccggtcagc gtcacgtcac
    48301 ctgggcgcgg ctacgcggcc tgaatgacaa acagaccgaa cgccgccata ttctgcgcaa
    48361 tgcctcgctg ccgatgatca ccgccgtggg gatgcacatt ggcgaattga ttggcgggac
    48421 gatgattatc gaaaacatct ttgcctggcc tggcgtcggg cgctatgcgg tgtcggcgat
    48481 ttttaaccgt gactatccgg tgattcagtg ctttacgctg atgatggtgg tggtttttgt
    48541 ggtctgtaat ttgattgtcg atttgctcaa cgccgcgctg gacccgcgca ttcgtcgtca
    48601 tgaaggagcg cacgcgtgaa ctttttcctc tcttcccgct ggtcggtacg cctagcgctg
    48661 atcattatcg ccctgctggc gctgattgcg ctcaccagcc agtggtggca gccgtacgat
    48721 ccacaggcaa ttgatttgcc gtcgtgcctg ctttcgccgg atgcgcagca ctggctgggc
    48781 accgatcact taggtcgcga tattttctcg cggctgatgg cagcgacccg cgtgtcgctc
    48841 ggttcggtaa tggcctgcct gctgctggtg ctgacattag ggctggttat tggcggcagc
    48901 gccgggttga ttggcgggcg cgttgatcag gccaccatgc gcgtcgccga tatgtttatg
    48961 accttcccga cctcgattct gtcgttcttt atggttggcg tgctcggcac cgggctgacc
    49021 aacgtaatta tcgccatcgc cctgtcgcac tgggcgtggt atgcacgcat ggtgcgcagc
    49081 ttggtgattt cactacgcca acgcgagttt gtgctggcgt cacggctttc cggtgcgggc
    49141 catgtgcggg tgtttgtcga tcatctggca ggcgcggtga tcccttcgct gctggtgctg
    49201 gcaacgctgg atatcggcca tatgatgctg cacgtcgcgg ggatgtcttt ccttggcctc
    49261 ggtgtgaccg cgccgaccgc cgaatggggc gtgatgatta acgacgcgcg ccagtatatc
    49321 tggacccagc cgctgcaaat gttctggccg gggctggcgc tgtttatcag cgtgatggcc
    49381 tttaacctgg tgggtgacgc actgcgcgat catctggatc ctcatctggt gacggagcac
    49441 gcacactaat gccgcaacag attgaactac gtaatatcgc gctacaggcc gcgcagccgc
    49501 tggtacacgg tgtatcgtta accctgcaac gcgggcgcgt gctggcgtta gtcggcggta
    49561 gcggcagcgg gaaatcatta acctgcgccg cgacgctggg cattttgccc gctggcgttc
    49621 gccagacggc gggggaaatt ttagccgatg gcaaaccggt ttcgccttac gccctgcgcg
    49681 gcatcaaaat tgccaccatc atgcagaacc cgcgcagcgc ctttaatcca ctgcacacca
    49741 tgcacaccca cgcgcgggaa acctgcctgg cgttagggaa acccgccgat gacgctacgc
    49801 ttaccgctgc catagaagcg gtggggctgg aaaacgccgc gcgcgtgctg aagctgtacc
    49861 cgttcgagat gagcggcggc atgttgcagc gcatgatgat tgcgatggcg gtgctgtgtg
    49921 aatcaccgtt tatcatcgcc gatgaaccga ccaccgacct cgacgtggta gcacaggcgc
    49981 gcatcctcga tctgctggaa agcattatgc aaaaacaagc gccgggaatg ctgctggtca
    50041 cccatgatat gggcgttgtg gcgcgtctgg cggatgacgt ggcggtgatg tcacaaggta
    50101 aaattgtcga acagggcgat gtagaaacgc tgtttaacgc ccccaaacat acggtgacgc
    50161 gcagcctggt ttccgctcat ctcgccctct acggtatgga gctggcatca tgaccttact
    50221 taacgtctgc ggcctttccc atcactatgc ccacggtgga tttagcggaa aacatcaaca
    50281 tcaggcggtg ctgaataacg tttccctgac cctgaaaagc ggcgaaaccg tcgccctgct
    50341 gggacgcagc ggctgcggaa aaagcaccct cgcgcggctg ctggtaggct tagagtctcc
    50401 ctcacaaggg aatattagct ggcacggcga atcgctggcg aaacttaatc gcgcccaacg
    50461 taaagccttc cgccgtgata ttcagatggt gtttcaggac tccatcagtg ccgtgaatcc
    50521 gcgcaaaacc gtgcgcgaga tcctgcgtga accgatgcgc cacctgctat cactgaaaaa
    50581 atccgaacaa ctggcgcgcg ccagcgaaat gctgaaggcg gtcgatctcg atgacagcgt
    50641 tctcgacaaa cgcccaccgc agttaagcgg cggccagctc cagctcgtct gcctggcacg
    50701 cgcgctggcg gtcgaaccga aattgctgat tctggatgaa gccgtttcta accttgatct
    50761 cgtgttacag gcgggcgtca ttcgcctgct gaaaaagcta caacaacagt ttggcactgc
    50821 ctgcctgttc atcactcatg acttacgcct ggtggaacgc ttttgccagc gagtgatggt
    50881 gatggacaac ggacaaatcg ccgaaaccca ggcggtggga gataaattaa ccttttcctc
    50941 tgacgccgga cgtgtgctac aaaacgcggt attacccgca ttccccgtgc gccgtcgcac
    51001 ctcagaaaag gtttaacgcc aatgcaacga gtcaccatca cgcttgatga cgatttactg
    51061 aagacgctgg acagcctgag ccagcgtcgt ggttataaca accgttccga agctatccgc
    51121 gatattctgc gtagtgccct ggcgcaggaa gctacccagc agcacggcac acaaggtttc
    51181 gcggtgctgt cgtatgtgta tgaacacgaa aaacgcgact tagccagccg tattgtttcc
    51241 acccagcatc atcaccacga cctctccgtc gccacgctac atgtgcacat caaccacgac
    51301 gactgtctgg aaatcgccgt gttgaaaggt gacatgggtg acgtgcagca ttttgccgat
    51361 gacgttatcg cccagcgcgg cgtgcggcac gggcatttgc agtgcctgcc gaaggaagat
    51421 tgagttattt ctaaaacaag gaaagaaaag atattaactg aacttaattt ataaaattaa
    51481 gttcagttgc atatatagat cctgtaaagg cagatattct gcgtaatgta cgtcgctaaa
    51541 atttataaat gattcaaacg ctcattcagt gtttcaataa tataggtatt aacagagact
    51601 tgttgctgcg cagccgcatt attaaggcgt tcactcagcg attctggata gcgtaacgta
    51661 aaggtcttaa tcttttctgt acgcacatac ggctcaatac ccgcagcttt acaatcctca
    51721 agatactctc gcagggaaag ctctccttct ttttgcagcc cctgaatact gtcagagaca
    51781 aaatcacaat aacctgacaa gcccaggaat ttgccacgaa atgcattcag ttctggcacg
    51841 taggtaataa cagctggttg cccggcaatc tccatagagt ttggcgtctt cagtttgatc
    51901 atggttttac ccctatactt agtaaccagt cacacacgct ttctacagcg cctttatcgg
    51961 tatctggcga cggatgaggc cgatggaaac acgcaacgct catatttagt atgaacttgc
    52021 aacgcgaacc tcttccttcc ttaatttctc cgcctaatgc tttaaccagt gactctatat
    52081 cagaccactt gatcccttga ggaacaggag ttttaaatat ttgatccaat gtattttttt
    52141 gtttcttccg taaagcctgt accttggttc tcatggcatg tccttatgac ttcactttat
    52201 gacatcacag tatgaagtca agtttccctt ccttacgcca ttgtcccaat cgtcttcctg
    52261 aatcgcagca gtgcgatggt gaaaaacgcg ccgccaattg ccatcagcgt caggaactgc
    52321 ggccagacga tttcgaatcc ggcaccccgg tagaggatgg cctgcgcgag gctaacaaag
    52381 tgtgtcgtcg gcatggtcag cataatgtcc tgcaccatct gcggcatact ttcgcgcggc
    52441 gtggatccac cggaaagcat ttgcagcggc agcagcacca gaatcaccag cagccccagt
    52501 tgcggcattg aacgcgctat cgtccccata aaaatgccga ttgacgtggt ggcaaacaga
    52561 ctgagcgcca cgcccagcat aaacagcggg atcgagcctt caatcggtac gcccagtaca
    52621 cctttcacca tcagcaccag cgataatccc gataccacca gcaccaccag ccccatcgac
    52681 cagatcttcg ccatcatgat ctcaaacggc gttatcggca tcaccagtaa atgttccacc
    52741 gtgccgtgtt cacgctcgcg gatcagcgcc gatccggtca atacaatcgc cagcatggta
    52801 atgttgttga tgatcgccat caccccgcca aaccacgcgg gatcaaggtt cgggttaaag
    52861 cgcatccggg tttccagcga taccaacggt tcgctgttat cacggtagcg cgcgacaaag
    52921 ctgttcactt caccgttgat aatattctgg atatacccat tgccggtaaa tgcctggctc
    52981 atgcgcgtgg catcgacgtt cacctgaata tccggctggc gtccggcgag gacatcacgc
    53041 tgaaaattag gcggaatatt tatcgcgaag gtatagcgtc cggcgtccag tccggcatcc
    53101 atctcatcgg cggtgatcat ctccggtggc aaaaaccacg gacgatagaa gctgttaacg
    53161 atccggttcg ataactgcga ttgatccata tcggcaatgg cgatcggcgc gaggttcaac
    53221 gatcctggcg taacggtcgc tgacgaatac accgacaccg taaacgagaa gacaatcagc
    53281 gtcagcatcg ctttatcacc gagcagactg cgcaactctt tgatacccag attaaaaata
    53341 ttgcgtaaat ggcgcatcat ccctcctgtt ttttcagcag caggatgctt aagcccatca
    53401 ccagcgggat ggctatcagc aacgggataa aaagttgcca caaatcagtc agatccagcg
    53461 ctttcgagaa cgttccgcgg gcgatagtca gaaaatgact ggtcgggtaa acctcgccga
    53521 tccaacgtcc aggcccttcc agcgaagcta ccggatcgat catcccggaa aactgtgtcg
    53581 ccgggatcaa tgtgataatc gccgttccga aaatggcggc aatctggctt ttcataaagg
    53641 tggagatcag cagccccatt ccagtggcaa tgatgatata cagcagcgcc gccaaggtga
    53701 gcgtcaggaa actgccttta tgcggtacgc caaacacaaa caccgacagg ccgcagagca
    53761 ggaaaaagtt cagcatcccc agcgcgatgt acggcagctg tttgccaagc aaaaattcgc
    53821 tacgggtggt gggggtcacg taaaggttga taatcgaccc tagctctttt tcccgcacca
    53881 cgctaagggc gcttagcatt gacgggatca tcatcagcag aagcgggatc accgccggaa
    53941 caatcgctgg caggcttttt acgtccgggt tatagcgata acgcgtctca atgttcatca
    54001 gcccgctttg gctggcgggt gtcgattgtc ggctcgccac atcctgaagc caactctggt
    54061 gcattgcctg cacgtaacct tttaccgttt cggcacgact gggcatcgct ccgtcgatcc
    54121 agacgccgag ttccacaggc gtaccacgcg cgatatcgcg cccgaaattg ggcgggatct
    54181 caatggccac cgtgatatcg cccgcacgca tccgacgatc aagctcgtca taactggtga
    54241 gcggcggctg ttcgataaag taacgggaac cggagaggtt gagtgtccac gcctggctac
    54301 tgacggtctg gtcgcggtcg agcaccgcaa agcgcaggtt ttccacatcc atactgatgc
    54361 cgtaacccat tatcagcatc aggatcaccg ttcccatcag cgccagcgtc gaacgtactg
    54421 gatcgcgtcg cagttccagc gcttcgcggc ggctgtagct aaacagacgg cgcaggctaa
    54481 atccctgacg cggcgcgtgg gtggtgtcgt gtaccacggg cggcgcttcg gcttcgttgc
    54541 tctgccctgc cgcttcctgc aaataggcga taaatgcctc ttccagactg gcggctccgc
    54601 gtttctcaac cagttcctgc ggtgtaccgc tggcaagcac ttttccggcg tgcatcagtg
    54661 agatgcggtc gcaacgttcc gcttcgttca taaagtgggt ggagatgaag atagtcactt
    54721 tgtcctggcg cgagagatcg accatcaact gccagaacat atccctcgcc accggatcga
    54781 caccagaagt aggctcatcg aggattaaca tctccgggcg atgaatcacc gccaccgcca
    54841 gcgaaagccg ctggcgaatg ccgagcggca atgactccgg cagaacatct tcaacgtcgt
    54901 tgagcttaaa acgctcgctc atttcagcca ctcgcgcggg aatttccgct tccgggatgt
    54961 gaaacaaacg ggcatgtaac tccaggtttt gccgcacggt gagttcgtta tagagcgaaa
    55021 acgcctgcga catatagccc acacgacggc gggtatcgat atcttttgga tcaaccggtt
    55081 gcccaaatag ccacgcctca ccttcgctgg cgggcagcag tccggtaagc attttcatgg
    55141 tggtggattt accgcagccg ttcgaaccaa ggaatccaaa aatctccccg cgtggaatgc
    55201 ggaaattgac gtgatcaacg gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt
    55261 cgatggcaat ctctgcgttt tcaggttgat acggtgggat cactaccgcc tgatgcgcct
    55321 ggcgttgcgc ttgcggtaac agattaataa atgcttcttc cagcgtggcg ctttgcgttt
    55381 gctgccgcag ctcttcggcg ctgccggttg ccagcacttc tccggcattc atcgctacca
    55441 gccagtcgaa gcgttcggcc tcttccatat aggcggtggc gaccagcacg ctcatattgc
    55501 tctgccgctg gcgaatactg tcgatcagat cccagaactg ggcgcgggag agcgggtcaa
    55561 cccccgttgt tggctcatca aggatcaaca gttccgggtc gtggattaac gcgcagcaca
    55621 gcccaagttt ttgcttcatc ccgccggaga gtttacctgc cggacgatcg cgaaacggtg
    55681 ctaacccggt gctggtcagc agctcattaa ttcgcacttc ccgctccgct ttatcgtgac
    55741 cgaacaggcg agcgaaaaaa tcgacatttt catacaccga caaggtgtgg tagaggtttt
    55801 tgcccagccc ctgcggcatc caggcgatgc gcgggcagac gtctcggcga tgcttcgggt
    55861 cgtgcatatc gccacccagc accaccacat tgccctgctc aatgacgcgg gcaccggaaa
    55921 tcagtgacaa taaactcgac ttcccgacgc catccggacc aatcagcccg accatgcaac
    55981 gggccggaat atcgagagtg atattgttca gcgcaacggt ttttccataa tgctggctca
    56041 cgcccgccag ttgcgcgaca ggcgggacgg gaaccagttc cagatgcgtc attgcggcaa
    56101 cctcaccacg aggtcgtcag gccacggaag ttcttcattc acccgcaccc acgctacgcc
    56161 cggtaaaccg gttttgacat attccagatg ctgctggagt aattccggtg ggatacgcgc
    56221 tttgacgcgg aacatcagtt tcagccgttc atcgctggtt tcgacggttt ttggcgtgaa
    56281 ctgggcgaca ctggcgacaa aactgatggt tgcaggaata cgcagatctg gcgcggcatc
    56341 gaggatcagc cgggcttcac cgcccagttt cagcgtgccc gcctgttcgg ttggcaggaa
    56401 gaaggtcata tagacgtcgc tgagatcgac catattcagc acccgaccgc ctgccgccag
    56461 cacttcccct ggctcggcaa cccgatactg cacgcgtccg tcacgcgggg ctttcagttc
    56521 actgtcatcg atatctgcgg caatgcgccg ttcagtggct tgcgctgctt cgacccgagt
    56581 ttgcgcctga atgatattgg tgcgtgctgc ttctatagcc gctttagaag ccgaaacctg
    56641 ggctttcgct gattccagcg cagctcgggc gctctcagcg gcggcgcggt cgtcatccag
    56701 ctgttgcgca gaaatagccc ctcgttgggc gagcgagcgg gaacgcgtat gacgtttggc
    56761 aacagagtcc agttcggcct ggcgttgatt gaccagcgac tgtgcggcac gagtttcact
    56821 ttgtcgttgc tccagcaaag cctgcgcggc ggcaacggcg ctttgtgcct ctttgatttg
    56881 cgcgatggct tccagtcgct gttcctgcaa cacgcgagta tccatcttcg ccagcacttc
    56941 gccttcgcga acaaactggc cttctttcac cagaatggtg tcgatacgcc cggcaatttt
    57001 gctggcgata tccacttccg tcgcttcaat gcgcccattg ctgacagcaa agccttccgg
    57061 cacacccgcc gggcgcaaca gccaccacgc cacgacggca gccaccgcca gtgccccgac
    57121 aacccaccac gccagatggc gcttactctt atccataatc gacccgccat aatccctgta
    57181 agcaaacgac gcgtgttaca actgccgccg aacgacgttc agcggattct tccatccggg
    57241 ataagaagga aacgacaaat acgaccagcg cgcagagcat tgagcgccaa cggggaaggc
    57301 ttagtgaaaa cgggtagtaa tgttgtcatc atcagcattc ctggccgtaa atgaaacacg
    57361 catactcata agtgtgatct ataaaactgg ctgacaaaac ggtatccatc ctgttggatc
    57421 gcatttcacg cctgttcagt tgcaggaaca gagcattcct ttttacgttt tatcaacagt
    57481 ctcaaccaga tagtaacatt aatattcatg ttgtctatgg ttcagagctg taacattgtg
    57541 cgccttctaa tcaaaggcat aagtccattt ctgttttttt catgctggta agacttccag
    57601 ggagtggtta tctctggccc tgcaccaggg ttaatttggc aattatgcat gctgaacatt
    57661 cttccagcat gtttactgtc acgggcgtta agacctaaaa ctgtccccgt ttcacctatg
    57721 cgttctctcc caccatttcc gttatgtgca acaacttgat ttatacctaa catttgtgaa
    57781 catgctaata gtgcttcaag tttaggtctg taatctgtcg aaacagaaaa ataacgatta
    57841 aaggatcgac aagagatagc ccggctagtt ttaaactttt tgccattaat tatagcatga
    57901 tgctttttat taataatttc cgctaattcg accggatcga gagcattggt catttcatca
    57961 atagagacag ttatcggtcc tataatgtaa cgttttcgct gatcatcaaa agttatccca
    58021 tgatgatttg ccattatttt gtatttgggg tcatagaaac atacaggata atcttttatc
    58081 ccgtcgtagg tatcctcatc aagtggtttc atgcgcgtga agttttgcat atagttacca
    58141 ttgcaattcg tttcatgatt tccagctaat acatgaacat tttcattgat actcattatg
    58201 cgttttaaca aatcgatgat aaattgatcg ccacagatat tactaaatcg atcgccggtt
    58261 tggtcgccaa gaaacgtaca tggcgtgact gcgtctttat taataaccaa ataaggctga
    58321 agactttcta aaacattatc tcttctgttc agtatactga ttattgtatt aacattagtg
    58381 tcaagttgct cattaaagtt ctcccttagc aatacgttga gaacttcatc ttctatatta
    58441 agcaactggc taaggaaatt ttctccttca gccatgattc ccaaatgtcc tgttgcaaaa
    58501 agcgcgtaca aaactgcacc gactgagcca tcggtatcgc caaaataggt cggcctggta
    58561 cttgtgggat caacaggtga acaattggag aaataggttc tgtcagcgaa ctggggtttt
    58621 ataattgttt cgaatttaat atcaaataca ttgtattgcg atatttccca gttccagatg
    58681 ggtttgtacg actcagtaac tgcaacatca ttagcattat catttactgt catattatta
    58741 agtaaataaa caatgctgca ccaattaata tcggtattat caagagggca accgtaattt
    58801 ttactattaa ttgtcacaat aaactgataa cagttattcc tacgtagaat atgagcgcgc
    58861 atgcccgata gcatactata tttttctgaa tgattcgttt gtatggtaaa tgaaattttg
    58921 tcatcttgaa tagattgttg gaaacgttct atttgccaat catcaatttt ttccacaact
    58981 tcaccgagca ttgtcctggc aatacttgat atatcatcac ctataccatt tgcaatatac
    59041 ccaaacagtt ctttagtgaa gttggtaaca tgctcttgca ccatatcatt tgttgcgatt
    59101 gtcgttgtac tgccattagt gcctgccaca gttccaattt tcatctttta cctcatatta
    59161 ttcaccgcga ggaatacata gttaacattt tatcttatga catacacctc atattcgatg
    59221 gggaaataat tatattttgc acaaatcatt cagccttaaa gcataatatg aaatttctta
    59281 tctcatagca gagaaagtcc ctaaagattg tagaggcgtc atcagtacat acctctacaa
    59341 tcgattaaga aaacctatga aatactggca aaattaaaaa gagacaccac agcactcatg
    59401 aagttttatt gaggttgaaa acaactaagc cctgggacac cttttttatc accacgttcg
    59461 ttaggtgcta tcttccaaga atttagcccc atggtataat tatcaatttt ttgatgctgg
    59521 tctaccccta atccataatg gccaaatttc tgtttagttt ttttgagcgt ctgtccacat
    59581 ttgaagatat cttcaggttt tggtctgaaa gcagtagtgt tattaaaata attttcatat
    59641 acaggtgtgg gtgctatagc attgcttcta attagatgga gaagatgatc atccatgtgt
    59701 tgtttatgtt ttttattaca tatatttgct aattcctcag gatcaattgg gtctttcatc
    59761 tgattaaatg gaacctgcaa agcaccgaga caataacatt gattttcttc atttcttatt
    59821 attccgtgat gactggttaa aactttagtt tctgagttat aattacaaac atcgagcgtt
    59881 ttaattaagt tatacgtatc accggcagag agtttataat tttctagtcg tgcagtatag
    59941 ttaccattaa aattgatttc atgatttcca gccagaacga tgacattttt attaatacgg
    60001 ctatctttat taccctccat atttcgcata gaatttagca aagtaaggat atatttatca
    60061 ccaaaaatag tactaaatct atcgcctgta tgatccccaa ggaagataca aggggttatc
    60121 gcatcgtcct ttaaaaaaac atgttgatgc aactcattca atatcatgtc tctcgtattt
    60181 aataaattgt agagcaattt attattttta tgtatgtatg cagatgaagc catgtcttcg
    60241 tgttttaata attcacataa taaactccaa cccttttccc tgatgcctat atgccctgaa
    60301 acgagcaatg aataaagtac agcgcctact cttccatctg tatcgccaaa ataagccgtg
    60361 ttggcatttt gtgggttaat cacaccacat tgtttataaa agtctatttt agaaaacaca
    60421 gatttaacat agctatcaaa cttatcctta ggaaacgtct ctttagagac atcccaacgc
    60481 cagtcagctt taaaattacc gctaagaaga gtaaagaaat tttcaggtat gatagaaagc
    60541 ccattaactt tataaattat gtctgattta tctccggaga aattaataaa ataataacca
    60601 tccttacact caatcttaac tgtaatggga gacgataatt ttatcacatg gttattgtct
    60661 ggtataattg taaagataaa cgtgttagta gtattatcgg caaaactatt atattcttga
    60721 atatctatag atggctcact attgtaaaac catattttct ccatctgaag actatgttca
    60781 tttaccctcc agtgaccatg ttgcaggaaa tagatgatat gcatgccaaa atcgttatct
    60841 atttgcgaat cattatccag cttatcaatt tcaactatct ttgaacgatg tgttttatca
    60901 accttgctat ggtgttgatg attattaact ttatctgaat gcttatcttt atggtttgaa
    60961 gggatgtact taaatatatt gtcaattgct ttcataaata acccgcgata tttttgacct
    61021 gttagtaaaa aataaatgat acatgtctga aagacatatt tacgacgata taaaacaata
    61081 atacttatat gttcgctgat tttaagcacc gagactatcc ggtcttttac taaataatgg
    61141 aaaacaatcc atccctggaa gacaattgta aaaaaaacaa cagaataaga attaatctta
    61201 ggataaatat ttatttatca tggccttttg accgctaaaa aatggcactc ggcacagtaa
    61261 accccgctat agttatgcta tgcatttgtc tttaatgata tctcgtcgtt atattaacag
    61321 gctgaaatta tcattaatgc acatttcaat attagcaggg atacctgagg agtttaaagt
    61381 gaacatatat atcgggtagc ttttcaaatt aatccctttg attataggct taatttgcat
    61441 cgcgttaggt ggctttgtgc tggaaagttc agggcaacgt cacgggtcat gtgctgattt
    61501 ctctggcggc catatgcctg gcattattca ctaccgcatt tattatcatt tcgcaactca
    61561 cgcgcggcgt taatacgttt tacaatacat tgttccccat tattggctat gcggggtcaa
    61621 ttatcaccat gatatggggt tgggcactgt tagcaggtaa tgatgtgatg gcagacgagt
    61681 ttgttgccgg ccatgttatt tttggcgttg gtatgattgc cgcatgtgta tcgacggtgg
    61741 cagcgtcgtc cggtcacttt ctgctcattc ccaaaaatgc agcggggagc aagagcgacg
    61801 gaacaccggt acaggcttat tcttcattaa tcggtaattg cctcattgcc gttcccgttt
    61861 tactcaccct gctcggtttc atttggtcta ttacgctgtt acatagtgcc gacataactc
    61921 cgcattatgt cgccttgggt taaccgcaat ctgtgcctgt ctaattggcc tagtggccac
    61981 aatcgtccat caaacacgta atacgttctc atccaaagag cactggctgt ggtgttattg
    62041 ggttattttt ctcggctcag tcacggtgct gcaggggata tacgtcttag tcagttccga
    62101 tgcaagcgcc cgactggctc ccggcattat tctcatttgc ctcggaatga tctgttacag
    62161 catattctca aaagtctggc tactggcact ggtctggaga cgtacctgtt cgttagccaa
    62221 cagaataccg atgataccgg tatttacttg tctgttttgc ttgtttttgt catcattcct
    62281 cgcggaaatc gcacaaactg agctggacta ctttattcca tctcgggttc tggttggatt
    62341 aggcgcagta tgttttacat tgttctctat tgtttcaata ttagaggccg gttctgcgaa
    62401 aaaaaattaa aatttacata atcccagcgt tattcatatc ttaacgctgg ttttttacga
    62461 acttagtgaa ataatgtaga atataaaatt ataattttat attggagcac atgtgttaat
    62521 gatcaacccc gggatactaa aaatatagac attattaaac ttgcttagcc tcgcattgcc
    62581 tttacctgtt aaagatgcat cgagaaaatt gagtctgaag ttctcatcct ttatctgatt
    62641 ttccagtccc gaaactttgg ccttagcatt acctgagatc cgtccgttac tatctatttt
    62701 gtaggtaaaa aaatacgaat gggaaaattt cctcggtgta tcttcatcag ttaagccaac
    62761 aatcgtaaaa ctaccgctac catctttttt taaaatgact gaaaggttag cagagtagta
    62821 taaagaatga tctgttttag catttcttgt taacgtcgac gtacattcaa gagacgttgt
    62881 gctaccatca cgagccttcc agagagcaaa aaaacacacc tgtagcaatg tgaaaacaag
    62941 tattaatata aaatacattt tcttattagt catataatag cacctctgaa caaattgcat
    63001 aagtttcgtc attagaggat tttatacatc ttgataaata ccgctgccct tccccaagca
    63061 aataatgcat actatttaca tcatatataa aatttgcacc tggcacacag ggtaaatgct
    63121 ttttcgaaat ttcagcggcc tctatttgag atttagcgag gccaatctcg ttaagattct
    63181 ctgaaagata taaatcacag gtgtcaatct tacctgattt aaccaatgga taatccgtta
    63241 tactgtaggt atctttaaaa gccacggcaa acatttcaat aataacaatt actaccacaa
    63301 gcgaaatcaa aatgcgaaaa agaggaatac tccttttgcg ctctactttt tcaacaacat
    63361 cttcttgtaa atgaga
//
LOCUS       DAJTRB010000011        58558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTRB010000011 DAJTRB010000000
VERSION     DAJTRB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.011"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..1102)
                     /locus_tag="OO330_000759"
     CDS             complement(<1..1102)
                     /locus_tag="OO330_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5881742.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLYAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1314..1661)
                     /gene="pspE"
                     /locus_tag="OO330_000760"
     CDS             complement(1314..1661)
                     /gene="pspE"
                     /locus_tag="OO330_000760"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5881743.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1704..1925)
                     /gene="pspD"
                     /locus_tag="OO330_000761"
     CDS             complement(1704..1925)
                     /gene="pspD"
                     /locus_tag="OO330_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5881744.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1934..2293)
                     /gene="pspC"
                     /locus_tag="OO330_000762"
     CDS             complement(1934..2293)
                     /gene="pspC"
                     /locus_tag="OO330_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5881745.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2293..2517)
                     /gene="pspB"
                     /locus_tag="OO330_000763"
     CDS             complement(2293..2517)
                     /gene="pspB"
                     /locus_tag="OO330_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5881746.1"
                     /translation="MNALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2571..3239)
                     /gene="pspA"
                     /locus_tag="OO330_000764"
     CDS             complement(2571..3239)
                     /gene="pspA"
                     /locus_tag="OO330_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5881747.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3406..4383
                     /gene="pspF"
                     /locus_tag="OO330_000765"
     CDS             3406..4383
                     /gene="pspF"
                     /locus_tag="OO330_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5881748.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4503..5768)
                     /gene="puuE"
                     /locus_tag="OO330_000766"
     CDS             complement(4503..5768)
                     /gene="puuE"
                     /locus_tag="OO330_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5881749.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5806..7086)
                     /gene="puuB"
                     /locus_tag="OO330_000767"
     CDS             complement(5806..7086)
                     /gene="puuB"
                     /locus_tag="OO330_000767"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5881750.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7088..8575)
                     /gene="puuC"
                     /locus_tag="OO330_000768"
                     /pseudo
     CDS             complement(7088..8575)
                     /gene="puuC"
                     /locus_tag="OO330_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(8725..9282)
                     /gene="puuR"
                     /locus_tag="OO330_000769"
     CDS             complement(8725..9282)
                     /gene="puuR"
                     /locus_tag="OO330_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5881751.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9309..10073)
                     /gene="puuD"
                     /locus_tag="OO330_000770"
     CDS             complement(9309..10073)
                     /gene="puuD"
                     /locus_tag="OO330_000770"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5881752.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10285..11703
                     /gene="puuA"
                     /locus_tag="OO330_000771"
     CDS             10285..11703
                     /gene="puuA"
                     /locus_tag="OO330_000771"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5881753.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11852..12016
                     /locus_tag="OO330_000772"
     CDS             11852..12016
                     /locus_tag="OO330_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881754.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12006..13391
                     /gene="puuP"
                     /locus_tag="OO330_000773"
     CDS             12006..13391
                     /gene="puuP"
                     /locus_tag="OO330_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5881755.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13525..13770
                     /locus_tag="OO330_000774"
     CDS             13525..13770
                     /locus_tag="OO330_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5881756.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14083..15726
                     /gene="sapA"
                     /locus_tag="OO330_000775"
     CDS             14083..15726
                     /gene="sapA"
                     /locus_tag="OO330_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5881757.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15723..16688
                     /gene="sapB"
                     /locus_tag="OO330_000776"
     CDS             15723..16688
                     /gene="sapB"
                     /locus_tag="OO330_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5881758.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16675..17565
                     /gene="sapC"
                     /locus_tag="OO330_000777"
     CDS             16675..17565
                     /gene="sapC"
                     /locus_tag="OO330_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5881759.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17565..18557
                     /gene="sapD"
                     /locus_tag="OO330_000778"
     CDS             17565..18557
                     /gene="sapD"
                     /locus_tag="OO330_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5881760.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18559..19365
                     /gene="sapF"
                     /locus_tag="OO330_000779"
     CDS             18559..19365
                     /gene="sapF"
                     /locus_tag="OO330_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5881761.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19433..19786
                     /locus_tag="OO330_000780"
     CDS             19433..19786
                     /locus_tag="OO330_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5881762.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="OO330_000781"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="OO330_000781"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5881763.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="OO330_000782"
     CDS             21085..22212
                     /locus_tag="OO330_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5881764.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="OO330_000783"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="OO330_000783"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5881765.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24394)
                     /locus_tag="OO330_000784"
                     /pseudo
     CDS             complement(24292..24394)
                     /locus_tag="OO330_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="OO330_000785"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="OO330_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26570..26743
                     /locus_tag="OO330_000786"
     CDS             26570..26743
                     /locus_tag="OO330_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881766.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26829..27578
                     /locus_tag="OO330_000787"
     CDS             26829..27578
                     /locus_tag="OO330_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5881767.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27847..28065
                     /gene="osmB"
                     /locus_tag="OO330_000788"
     CDS             27847..28065
                     /gene="osmB"
                     /locus_tag="OO330_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5881768.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28191..28517)
                     /gene="yciH"
                     /locus_tag="OO330_000789"
     CDS             complement(28191..28517)
                     /gene="yciH"
                     /locus_tag="OO330_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5881769.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28517..29254)
                     /gene="pyrF"
                     /locus_tag="OO330_000790"
     CDS             complement(28517..29254)
                     /gene="pyrF"
                     /locus_tag="OO330_000790"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5881770.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29447..30616)
                     /gene="lapB"
                     /locus_tag="OO330_000791"
     CDS             complement(29447..30616)
                     /gene="lapB"
                     /locus_tag="OO330_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5881771.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30623..30931)
                     /gene="lapA"
                     /locus_tag="OO330_000792"
     CDS             complement(30623..30931)
                     /gene="lapA"
                     /locus_tag="OO330_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5881772.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31080..31844)
                     /gene="pgpB"
                     /locus_tag="OO330_000793"
     CDS             complement(31080..31844)
                     /gene="pgpB"
                     /locus_tag="OO330_000793"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5881773.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32014..32604
                     /gene="ribA"
                     /locus_tag="OO330_000794"
     CDS             32014..32604
                     /gene="ribA"
                     /locus_tag="OO330_000794"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5881774.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32668..35343)
                     /gene="acnA"
                     /locus_tag="OO330_000795"
     CDS             complement(32668..35343)
                     /gene="acnA"
                     /locus_tag="OO330_000795"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5881775.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTIVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGGERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35506..35601)
                     /locus_tag="OO330_000796"
     CDS             complement(35506..35601)
                     /locus_tag="OO330_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881776.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35715..35882)
                     /locus_tag="OO330_000797"
     CDS             complement(35715..35882)
                     /locus_tag="OO330_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881777.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35885..36049)
                     /locus_tag="OO330_000798"
     CDS             complement(35885..36049)
                     /locus_tag="OO330_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5881778.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36344..37318)
                     /gene="cysB"
                     /locus_tag="OO330_000799"
     CDS             complement(36344..37318)
                     /gene="cysB"
                     /locus_tag="OO330_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5881779.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37528..40125)
                     /gene="topA"
                     /locus_tag="OO330_000800"
     CDS             complement(37528..40125)
                     /gene="topA"
                     /locus_tag="OO330_000800"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5881780.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40505..40756
                     /locus_tag="OO330_000801"
     CDS             40505..40756
                     /locus_tag="OO330_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5881781.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40792..41841)
                     /gene="sohB"
                     /locus_tag="OO330_000802"
     CDS             complement(40792..41841)
                     /gene="sohB"
                     /locus_tag="OO330_000802"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5881782.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42061..42819
                     /locus_tag="OO330_000803"
     CDS             42061..42819
                     /locus_tag="OO330_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5881783.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42816..43406
                     /gene="cobO"
                     /locus_tag="OO330_000804"
     CDS             42816..43406
                     /gene="cobO"
                     /locus_tag="OO330_000804"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5881784.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43446..44318)
                     /gene="rluB"
                     /locus_tag="OO330_000805"
     CDS             complement(43446..44318)
                     /gene="rluB"
                     /locus_tag="OO330_000805"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5881785.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44419..45039)
                     /locus_tag="OO330_000806"
     CDS             complement(44419..45039)
                     /locus_tag="OO330_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5881786.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45036..45917)
                     /gene="yciV"
                     /locus_tag="OO330_000807"
     CDS             complement(45036..45917)
                     /gene="yciV"
                     /locus_tag="OO330_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5881787.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46055..46099
                     /gene="trpL"
                     /locus_tag="OO330_000808"
     CDS             46055..46099
                     /gene="trpL"
                     /locus_tag="OO330_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5881788.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46076..46169
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46191..47753
                     /gene="trpE"
                     /locus_tag="OO330_000809"
     CDS             46191..47753
                     /gene="trpE"
                     /locus_tag="OO330_000809"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5881789.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLC
                     AIATAHHAQETF"
     gene            47753..49348
                     /gene="trpD"
                     /locus_tag="OO330_000810"
     CDS             47753..49348
                     /gene="trpD"
                     /locus_tag="OO330_000810"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5881790.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49349..50710
                     /gene="trpCF"
                     /locus_tag="OO330_000811"
     CDS             49349..50710
                     /gene="trpCF"
                     /locus_tag="OO330_000811"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5881791.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQLGIKDARLLASVFQTL
                     RAY"
     gene            50722..51915
                     /gene="trpB"
                     /locus_tag="OO330_000812"
     CDS             50722..51915
                     /gene="trpB"
                     /locus_tag="OO330_000812"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5881792.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51915..52721
                     /gene="trpA"
                     /locus_tag="OO330_000813"
     CDS             51915..52721
                     /gene="trpA"
                     /locus_tag="OO330_000813"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5881793.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53102..53281
                     /locus_tag="OO330_000814"
     CDS             53102..53281
                     /locus_tag="OO330_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5881794.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53367..53867
                     /locus_tag="OO330_000815"
     CDS             53367..53867
                     /locus_tag="OO330_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5881795.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53913..54419
                     /locus_tag="OO330_000816"
     CDS             53913..54419
                     /locus_tag="OO330_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5881796.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54479..55117)
                     /gene="ompW"
                     /locus_tag="OO330_000817"
     CDS             complement(54479..55117)
                     /gene="ompW"
                     /locus_tag="OO330_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5881797.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55357..55446
                     /locus_tag="OO330_000818"
                     /pseudo
     CDS             55357..55446
                     /locus_tag="OO330_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55474..56217
                     /locus_tag="OO330_000819"
     CDS             55474..56217
                     /locus_tag="OO330_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5881798.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56247..56786
                     /locus_tag="OO330_000820"
     CDS             56247..56786
                     /locus_tag="OO330_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5881799.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56891..57289
                     /gene="yciA"
                     /locus_tag="OO330_000821"
     CDS             56891..57289
                     /gene="yciA"
                     /locus_tag="OO330_000821"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5881800.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57329..58045)
                     /gene="tonB"
                     /locus_tag="OO330_000822"
     CDS             complement(57329..58045)
                     /gene="tonB"
                     /locus_tag="OO330_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5881801.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPV
                     KKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            58260..58556
                     /locus_tag="OO330_000823"
     CDS             58260..58556
                     /locus_tag="OO330_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5881802.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14058 a        15140 c        14640 g        14720 t
ORIGIN      
        1 ctaatgcacc ttcctgctgt aatgcctcga ccagcgctaa tatttcgctt tctggaagca
       61 agccacgcag tgggttcaac ccgatgccat cgtgagaggc gaggaagtta aaccaggtcg
      121 ttttactgga gggcaagctc agactttgcg cccactggca cagcgcctcg acgttctgtt
      181 tttgcaccgc atataacacc agcggcggca gcgagaactg gtacaccata tgcgcttcgt
      241 catcaccgtt gccaaagtaa gcaatgttgt ctttgtgcgg aacattggtt tcggtaatga
      301 tcactgtacc tggtgctacg tcatcaataa tcgaccgtaa cagcttgata atcagatgtg
      361 ttttttccag atggatgcag cttgttcccg gctctttcca cataaagcca acggcatcca
      421 ggcggacata ctcagcacct ttctcaaggt aacagagtaa aacatccacc atcgccagca
      481 acacttcagg gctacggtaa ttcaggtcaa tttgatcgtc actaaaggtg gtccataaat
      541 ggcgcgtgga attatcatcc atttgaaacg gcgttaatag cggtaacgca cgcggaaggg
      601 tgacggcact gagatcggtt ttcggatcaa ccgcaataaa gaaatcttca aaacccggaa
      661 gctgttgtaa atagttttta aaccattcac tttttgcaga catatggttg cagacaaaat
      721 caaacattaa atgactgcat tcaccgagtt gctgaatatc ctgccactcc cccgcttcac
      781 tggcgacctg atgataatca attaccgaaa atccatcatc agatgaccat gggtaaaaag
      841 gtaataaatg aacatgtgaa aaaatgcttt gcagccattg atggtagaac tgattaaata
      901 tgggtagtgg ttttaaatca ttactgtgaa attgatcggc ataggtaatt aacacgacat
      961 cactttcatc ccagtgtttt ttacgtcgct gtgtaattaa tcgtttcgca ctctcaagac
     1021 gcgttacaag tttctgtaaa tgagttgcgg taaatgttcc accgtagatt tcgtccatgt
     1081 aatccgtaat tttctgtttc atgttgtctt tccgtggtag cggtcccatg aggcgagaga
     1141 ctactccgca gaaaataaac tgtcaattga gcacaagggt tttagtcacg caggaaatca
     1201 ttttttacca gggaaaaagc gtatttgtga gctgacgcaa ataatctata gggatgtaaa
     1261 taaggtgtga cagaaaaaaa acggcgcata agcgccgctc atggtgaatt cttttaacct
     1321 ttgaccttcg gcattgcgat gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc
     1381 atctcgctaa ggagctcttt tgcctgcccg gactggcgtc cggcattgca atacactttc
     1441 acggtgtcgt ttttatccgg aacggcggtg gcaatgcgct ctttcacttc cttcagggga
     1501 atattgatgg ccccctgaac gtgctcttgc tgatactgct ctggaacacg aacatcgatc
     1561 cagtgttcag cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag taagcctttt
     1621 tttaaacatg taagcgtcct tctatggatg aaaattgcca tccattatac gcctgagatt
     1681 taacaatttt tgcgaaactc ccctcacctt ttataacgct gtgccagttt attagcagcc
     1741 cgacttaaca gcggttccag cgccacagcc agcaacattt tcagcggtcg gcgagcaact
     1801 gattttatcg cccagcccgc cacacccgcc gggccatagc gcagtgcggt aagaagtacc
     1861 agcttgcctg ctaatttgaa accaagcttc accttttgcc cggcctgttg ccagcgagta
     1921 ttcataactt tcctcacagt tgacggaaac ggctacgtaa cgtgaaagta tcggaagtga
     1981 cataacgttc catctcgcgt aaacgcgttt cacttgccgc cagttcacgg tcgacttcat
     2041 ccagcaattc gctgctggaa ggtagctgct caccaaaggc catgttgtcc ggcattggat
     2101 caagcgcaaa tgacaaaatg atgtaagcaa ccagggtaaa cagcgccaga ccgaagaaaa
     2161 tcgacagcac caccaggata cgcaccagtt ttaccggtac atcaaaatag ttggcaatcc
     2221 cggcgcagac gccgcggacc atgccctgct gtggaatacg ccataacttt ttattgagat
     2281 taatgcccgc cattagcgat ccctccagtt cggatgttct gcatcaagaa tagattccag
     2341 cgcctgaata cgttcgcgca tccgttttgc ttcatcaacc agttgcgcta atcgctgctg
     2401 ctcactttgc gacaattcac tgcgaccaga acgattgctg taatgcagcc ataaccagat
     2461 cggtaaaaca aacagcacaa aaatggttaa cggaatagcc agaaatagcg cgttcataag
     2521 tactccttag acgatgagcg gcggcgcgtt cggacgccgc ctggatgtta ttattgattg
     2581 tcttgcttca ttttggcttt taattgtgcc agttgttcgc tgattgcatc atcggctttc
     2641 agttcggcaa actgatcgtc cagcgatttt tgtttaccga agctgtggct ttctgcttcc
     2701 gcttccatct ggtcaatacg acgttcgaaa gattcgaaac gagccattgc ttcatccagt
     2761 ttgccactgt ccagctgacg acgcacatcg cgcgacgagt ttgccgcctg atgacgtaac
     2821 atcaatgcct gctggcgagc gcgtgtttcg ctcaatttgt tttccagctc gccaatctct
     2881 ttcttcatgc gtgccagcgt atcgtccacc agcgtcactt catgttccag ggacttaatc
     2941 agatcggtca gtttctgttt ttcaattaac gctgcacgtg ccagatcctc tctctctttc
     3001 agcagcgcca gttcggcttt ttcctgccat tcaacttcac gcgccgacgc ttgttcaata
     3061 cggcgagtca gctgtttctt ttctgccaac gcacgcgccg aagtagaacg tacttcaacc
     3121 agtgtatctt ccatctcctg gatcatcaga cgcaccagtt tctgtggatc ttccgctttc
     3181 tctaacagag cgttgatgtt ggcattcacg atgtcggcaa agcgagaaaa aatacccata
     3241 atgttgtcct cttgatttct gcgttcagga ttgtcctgct gaactgttaa tacaatttgc
     3301 gtgccaattt tttatctttt tgatttataa agatctgatt gaagaatcaa cagcaacatg
     3361 ccaggatgaa ttagcgaatt acactaacaa gtggcgaatt tcatcatggc cgaatacaaa
     3421 gataatttac ttggtgaggc gaacagcttt ctcgaagtgc tggaacaggt ttcgcatctc
     3481 gcaccgctgg acaaaccggt gctcatcatc ggcgaacgcg gcaccggtaa agagttgatt
     3541 gccagccgcc tgcattatct ctcctcccgt tggcaagggc cgtttatttc ccttaactgc
     3601 gcggcgttaa atgaaaatct gctggattcc gaactgtttg gtcacgaagc gggggcgttt
     3661 accggtgcgc aaaaacgtca tcctgggaga tttgaacgtg ccgacggcgg tacgctattt
     3721 cttgatgaac tcgctacggc accgatgatg gtgcaggaaa aattattgcg tgtgattgag
     3781 tacggtgaac tggagcgcgt tggcggtagc cagccgttgc aggtgaatgt gcggttggta
     3841 tgcgcgacga atgccgatct cccggcgatg gtcaatgaag gtacttttcg cgctgacctg
     3901 ctcgaccggc tggctttcga tgtagtgcaa ctgccgcccc tgcgcgagcg cgaaagcgac
     3961 attatgctga tggcagaaca ctttgccatt cagatgtgtc gggaaatcaa gctgcctctg
     4021 ttcccggggt ttacggagcg cgccagagaa acattgctga attatcgttg gccgggaaat
     4081 attcgtgaat tgaaaaacgt ggtggaacgt tcagtgtatc gccacggcac cagcgattat
     4141 ccgcttgatg acatcattat tgatcccttt aaacggcgtc cacctgaaga agccatcgcc
     4201 gtttcagaaa atacctcgct tccgacgctg ccgctggatt tacgcgagtt tcagatgcag
     4261 caggaaaaag agttgctgca actcagtttg caacagggga aatataacca gaagcgcgcg
     4321 gctgaattac tggggttaac ttatcatcag ttccgcgcgt tgttgaaaaa gcaccagatt
     4381 tagcgaagtt gagcaataca acttgccgga tgcggcgtga atgctttatt cggcatacat
     4441 aatcaatacg tcgtttgcag gcctgataag cgcagcgcat cagacattat tgcgttgggc
     4501 tattaatcgc tcagcgcatc ctgcaaaatt ttcattgccg catcgaattg cgcatccggg
     4561 atggtcagcg gatacaggaa gcgaatcacg ttgccgtatg cgccacaggt cagcaggagc
     4621 agcccctgcg ccagcgcgcg ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc
     4681 gtttgcggat cgttaaactc tgccgcaatc atcgacccca ggccgcgtac cgcagcaatg
     4741 gccggaacgc tttctttggc atcaatcaac gtgtttttga gacgctggcc cagttgattc
     4801 gcgcgttcgc agagtgattc tttgtcgata atattgagca ccgcgtgcgc ggcagccacc
     4861 gccagcgggt tcccggcgta ggtgccaccc aacccgcccg gcgcgggcgc gtccataata
     4921 ttcgcgttac cgaccacgcc cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc
     4981 attaagtccg gcttatcggc gtaatgatcc atggcaaaca gtttaccggt acgcgcaaag
     5041 ccgctttgca cttcatcggc aatcatcaca ataccgtgct cgtcgcacag gcggcgaatg
     5101 gcggcaacca gctcttttgg cgcaacgttg aaaccgcact cgccctgcac cggttcgaaa
     5161 ataatggccg cgacctgctt cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg
     5221 agggagtcct gtgttgaaat gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa
     5281 ccagggaacg ggccgaagcc gattttgtac ggcgcaactt ttccggtcag cgccatggtc
     5341 atatatgtac gaccgtggaa gccaccgcta aacgcaatca cgccaggtcg tccggtatgg
     5401 gcgcgagcaa ttttcaccgc gttttccacc gcttccgcgc cggtagtgaa gaatgcggtt
     5461 ttagcctgcc cgctcactgg ggcaagggtg ttaattttct ccgccagcgt gacgtagctt
     5521 tcgtacggca caatctgata cgccgtgtgg gtaaactgtt gcaattgctg ctccaccgcc
     5581 gcgaccagat cagggtggcg atgcccggta ttcagcaccg caatgcctgc ggcgaaatcg
     5641 atgtattcgt tgccctctac gtccttcagc gtagcgtttt ctgccgactg ggcgaagaag
     5701 ttacacatca cgccaacccc gcgtggggta gcagaaagac gacgctgatg gaattcattg
     5761 ttgctcatgt ttcacacctt ttgactcttc actgttaatc ggaaattaaa cgcccagacg
     5821 atcgcgcagg ctgtaataag ccgcgcccat cgcggtaaat ggcacgcgca gcgtgcgccc
     5881 gccggggaac gggtaatgcg gcagattggc gaaggcatcg aaacgctcgg cgtcaccgcg
     5941 cagcagttca gcaatcaaac gcccggcaag atgggtacag gtgacgccgt ggccgctgta
     6001 accctgcatg taatagatgt tggtatcaag gcggccgaac tgcggcatgt gcgacaaggt
     6061 cagcaggaag ttgcccgtcc agcggtaatc aattttcacg cccttcaact gagggaaggt
     6121 ttttagtagt ttcggtacca caagacgctc aacatcatcc gggttgcgtg caccatacac
     6181 tacacccccg ccgtacagca ggcggttgtc ggcggtaaga cggtagtaat caagcagata
     6241 gttacagtct tccacacagt agtttttcgg gatcagcgaa cgggctaaat cttccgacag
     6301 ccgttcggtg gtgatcacct gcgtgccgca cggcatgctg cgtttcgcta gttccggctc
     6361 tactttatcg cccagatacg cattcccggc gacaatcaca tacttcgccg tcacctgacc
     6421 attggcagtc cgaaccacgg ctggcgtggt gtgctggatc tgcgtcacgg cggaaagctc
     6481 atacacgcgc ccgccgttca ggcggatggc atcggcttca ccaatggcga ggtttagcgg
     6541 gtgaatatgc ccaccgctgt gatccagcag cgcaccagtg tagcgatcgc tggcgacctc
     6601 gcggcgaata gcgttgacgt ccagcagctc cagctgttta ttgccgtagc gttcccagtt
     6661 ctctttctgc tcttcgagtg tggcgagttg tttatcattc atcgccacaa acagaccgcc
     6721 ggggcggtag tcgcagtcaa tctgataacg tttgatacgt tcgcggatga tctcgccacc
     6781 ctcgaacatc atgctgccga gcatacgggc ggtgtccatg ccgtagcttt tttcgatcac
     6841 gtcgatgtcg cggctgtagg agttcacaag ctgcccgcca ttgcgcccgc ttgcgccaaa
     6901 gccgatgcgt gaggcttcga gaaccactac gtcaaagccc gcttccgcca gatgcagcgc
     6961 ggaggagagc ccggtatagc cgccgccaac cacgcaaacg tcgcaggtga ttgactcatt
     7021 cagtgtgtcg aatggtgcat atttattcgc actggcggcg tagtaactgc tggtatgttc
     7081 ggtcatttca ggcctccagg cttatccaga tggttttcag ttcagtgaat ttttcaaggg
     7141 catgcaggga tttgtcgcga ccgttgccgc tctgcttata gccgccaaac ggcacggtca
     7201 tatcgccgtc gttgtagtta ttgacgaaga cggaaccggc tttcaggcgt cggctcatgc
     7261 ggtgcgcgcg ggagaggtcg cgcgtccata ccgccgcgcc aaggccgtac tggctgtcgt
     7321 tggcaagctg cagcgcctga tcttctgatg tgaaacgggt gaccaccagc accggaccga
     7381 aaatctcttc gcggcttaag gacgcattcg ggtccacatc cacgaagatg gtcgggccga
     7441 tggccgctgc cagcccggcg ttgcgaccat ccagcagcag ttgccccttg ccttcgcctt
     7501 cacgaataaa ggtgtgtacc gagtcagcgt gagcgctgtc gattaaggtg cccatggtgg
     7561 ttgcgggatc aagtggatgg cccggctgcc agttttgcgc ctgctgtttt aacagggcta
     7621 agaattcatc ggcgatgctc tcttccagta acaaacgcgt tccggcgatg cacacctggc
     7681 cctggttgta gaagatgcct gcggcggtgg cgcttgccgc tttttgcaga tccggacaat
     7741 cggcgaaaac gatgttggcg cttttgccgc ccgcttccag ccagacacgt ttcatgttgc
     7801 tatcacccgc gtctttcagc agttgtttac ccgtacgcgt ggagccggta aaggcgatgg
     7861 catcgatatc gttatgacgc gaaagcgcct gccccgcttc atgaccaaaa cccgtcacca
     7921 cgttcaacac accatccggc aagcctgctt ctttcgccag ccccgcgaga cgaatcgcac
     7981 tgagcggtga tttttcagac ggttttagaa tcacgctgtt tcccgccgcc agcgccgggc
     8041 cgagtttcca gcaagtcagc aacagcggga agttccacgg cacgatggcg gcaatcacgc
     8101 cgaccggttc acgcacgatc atcgccagct catggctact ggtggtcgcc acttcgccat
     8161 acactttgtc gatcgcttcg gcgtaccagc gaatggcgcg cgccgcgccg ggaatatcat
     8221 cacgcagact gtgacgaatc ggtttgccgg tgtcgagagt ttccagcagt gccagctctt
     8281 cggcgtgggc ttccattaaa tcggcgagtt tattcagtac cgctttacgt ttagccggag
     8341 aagagagtga ccagtcgccg cgttcaaata cgccgcgtgc tgcgctcacc gcacggtcga
     8401 tatcgacgct cttgccgcgg gcaattttcg ccagcggtgc ctgggtgacc ggatcaacgg
     8461 tttcaaaggt ttcattttcc gccgcagcag tatattcacc gttaataaat aagcggtttt
     8521 caatggcgag acttaacgct ttatcctacc agtaagccag atgatgaaaa ttcattatga
     8581 ctcctgtttc acgtctatca gatatatgca gtttgtgaac gtcagggcgc agagatcccc
     8641 gggctgcatg cccgtttatt tctggcgaca ttctttatgg attgccgggc gcattgcgct
     8701 tacccggctt acagaacaaa aagattaaaa cgtggtgggc gtatgggcgc tgataattcg
     8761 gcaaatacct gccgacgtat tactgaaact gtgcgggatg ccggtattaa tggcataact
     8821 ttgccccgcg acgaggtggt aatcctgacc attaatcgtc agaacaattt caccttccag
     8881 tactgtgcct atttcctcac cctgatgctt aattctttcc ccagtggttg tgcccggctg
     8941 gtacgtttca aagatcatcg ccagcgtgcg attcgggtta ccgttatgaa ccagcttcat
     9001 tgacacaccc tgactgccca tctcaattaa gtcgtcctga ttaatgacga cctgcggctc
     9061 atcaggtttt tccggctcgg aaaagaattc cgagagtgac agaccataca ccttcagcag
     9121 cttttgcagc gtactgatgg cagggctgac tttatcttgt tctatcgtac tgatagcact
     9181 gtgagtcagc ccggagagtt cggcggcacg acgttgtgaa agcccctgct gctggcggat
     9241 ttccgacaag cgttttcctg gcgccagtcc ctcatcactc atatttgcat ttccttaaac
     9301 tgtagtggtc agagtcgctg tttttcagcg atatggtgct gccaagcggt gataaagccc
     9361 tcgaacaata tacgcgaaag cgcgtactcg ctactgttcc attccgggtg ccactgtacg
     9421 cccagcgcaa aaggatgatt gatgacgcta accgcctcaa ctaagccatc cggcgagcga
     9481 gcttcaacac gcaaccgtgg gctaacgacc ttcgcccctt gtccatgtag agagtttacc
     9541 caaaagttgc tacattcagg taacaacgca gacagtaatc ccccctcttc aacctgaact
     9601 tcatgcgacg gtgcatattg ctgttccacc ggcagttcgg gatcttcccg atgttccagc
     9661 aattcaggct gttcgcacag cttgcgatgc aacgaaccac cggttgccac caccagttct
     9721 tgtaaacccc ggcagatggc gaaaatgggg atgcgccttt cgagtgcggc attaattatc
     9781 gccatgctca gaagatcacg cccgggatcg gcgtcaggct catcgccgtt ttcaccatat
     9841 aggtgcggct gcacattgct gggactacta ggaagataaa tgccatcgag tttcggcaaa
     9901 agttgttcaa gtaacgacgg ttccgccagc gcatgtggta gcgcaattgg caagccgcct
     9961 gcgtggatga tggcattcag gtacttttct tgcagagtct gggtcgcatg acccttaagc
    10021 ctgttcctgc acattacgac accgataacc ggattgttca ttatattttc catgctcaat
    10081 ctcacaaagt ggactaaatt atcgccatta ctgcgcttta aatgaccttt atgttcaata
    10141 ttttttcaat ctagcagtgg attttcattt ttgcaaactc aatttaacat ttaacaaaca
    10201 tttggtttgc atacagattc gaatgatggt cattatattt tacgctttga taacgagcgg
    10261 aaaacaaacc aaaggcgaag aatcatggaa accaatatcg ttgaagtaga gaactttgtt
    10321 cagcagtcag aagagaggcg gggtagcgcc tttacgcagg aagtgaaacg ctacctggag
    10381 cgctacccga atacgcaata tgttgatgtt ttacttactg atttgaatgg ttgcttccgt
    10441 ggaaaacgta tccctgtttc aagcctgaag aagctcgaga aagggtgtta tttcccggcc
    10501 tcggtatttg caatggatat tctgggcaac gtagtagaag aagcaggtct gggtcaggaa
    10561 atgggcgagc cggatcgtac ctgtgttcct gttctcggtt ccttaactcc ttctgccgcc
    10621 gatccagagt ttatcggtca gatgctcctg accatggtcg atgaagatgg cgctcccttt
    10681 gacgttgagc cgcggaacgt tcttaaccgc ctgtggcagc agctgcgcca gcgcggattg
    10741 ttcccggttg tagcggtaga gctggagttc tatttactgg atcgccagcg cgacgctgaa
    10801 ggggatctgc aaccgccctg cgcgcctggc accgatgacc gcaatacaca aagccaggtt
    10861 tactccgttg ataacctcaa ccacttcgcc gacgtgctca atgatattga tgaactggcg
    10921 cagttgcagc tgattccggc agatggcgcg gtcgctgagg cctcgccggg tcagtttgaa
    10981 atcaaccttt accatactga taacgtgctg gaagcctgcg atgatgcgct ggcactaaaa
    11041 cgcctagtgc gtctgatggc agaaaagcat aagatgcacg ccacttttat ggcgaagccg
    11101 tatgaagagc acgcgggcag cgggatgcat atccatatca gtatgcaaaa taatcgtggc
    11161 gagaatgtgc tttctgacac agaaggcgaa gattcgccgc tgctgaaaaa gatgctcgcc
    11221 gggatgattg acctgatgcc gtcgtcgatg gcgttgctgg caccaaacgt gaactcgtat
    11281 cgccgcttcc agccgggaat gtatgtgccg acgcaggcgt cgtggggcca taacaaccgc
    11341 accgtcgccc tgcgtattcc gtgcggtgac cgtcataatc accgcgtgga atatcacgtg
    11401 gcgggtgccg atgccaaccc atatctggtg atggcagcga tttttgccgg tattttgcat
    11461 ggccttgata acgagctgcc gttgcaggaa gaagtcgaag gcaacgggct ggaacaggaa
    11521 ggcttaccct tcccgattcg ccagagcgat gccctgggtg agtttatcga gaatgatcac
    11581 ctgcgccgct atttaggcga acggttttgc catgtgtatc acgcctgtaa aaatgatgag
    11641 ctgttgcagt ttgagcgcct catcacagaa acagaaattg agtggatgtt gaaaaacgcg
    11701 taattcccct tgcggggttg tttcatcggc ctgagcggag ccaggcctga catttgctgg
    11761 taatccagac atttcccgga tgtcgcgtta tggagcgcag gcggcaacgg gcttccgtta
    11821 acgattaaga ctgtgacgag gaaatgagat gatgccgatg attaaatccc cgcatggcga
    11881 gggaggctgc gtatgcgccc ctcctgctac tgactggact ccgccccctt tgcttccctt
    11941 gctaaacagg tttgatttca ggtcgacacg accgcaaacc ttattacgca ggggaggcag
    12001 caattatggc tattaattca ccactgaata ttgctgcgca acccggcaaa acccgtctgc
    12061 gaaaatcact gaaattgtgg caggtggtaa tgatgggtct ggcctatctc acgccgatga
    12121 ccgtatttga tacttttggt attgtctccg gcattagcga cggtcacgtt ccggcgtcct
    12181 atttgctggc gctggcgggc gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc
    12241 agtttccgga ggccggttcg gcctatacct acgcgcaaaa gtcgattaac ccgcacgtcg
    12301 gatttatggt cggctggtca tcactgctgg attatctctt tttgccaatg atcaacgttt
    12361 tgttggcgaa aatctatctc tccgccctct tcccggaagt gccgccgtgg gtgtgggtgg
    12421 taaccttcgt cgccatttta accgccgcga atctgaagag cgtcaacctg gtcgctaact
    12481 tcaataccct gtttgtactg gtgcaaatct ccatcatggt ggtgtttatc ttcctggtgg
    12541 ttcagggact gcataaagga gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca
    12601 gcgagaacgc gcacctgatc ccgattatta ccggtgcgac gattgtctgt ttctcgttcc
    12661 tcggtttcga tgcggtgacc acgctttcgg aagagacgcc ggacgccgca cgcgtgatcc
    12721 cgaaagccat cttcctgacg gcggtatacg gcggcgttat ctttatcgcg gcgtcgttct
    12781 ttatgcagct gttctttccc gatatcagcc gctttaaaga cccggacgcc gcactgcctg
    12841 aaattgcgtt gtacgtcggc ggcaagctct ttcagtcgat tttcctctgc accacgtttg
    12901 tgaacacatt agcgtctggc ctggcttcac atgccagcgt gtcgcgtctg ctgtatgtga
    12961 tggggcgtga caatgtgttt ccggagcgcg tgtttggcta tgtgcaccca aaatggcgga
    13021 ctccggcact caacgtcatt atggtcggga ttgtcgcgct gtcggcgctg ttctttgatt
    13081 tagtcaccgc gacagcactg attaacttcg gtgctctggt ggcgtttacc ttcgtgaatc
    13141 tgtcggtgtt taatcatttc tggcggcgta aaggaatgaa taaaagctgg aaggatcact
    13201 tccactattt gctgatgccg ctggttggcg cgctgacggt gggtgtgctg tgggttaacc
    13261 tagagtcaac gtcactgaca ctcggtctgg tatgggcttc gttaggcggc gcatatttgt
    13321 ggtatctgat ccgccgctat cgcaaagtgc cgctgtacga aggtgacaga acgccggtga
    13381 gcgaaacgta atagccggat gcgcggcgca ttatcctgcc tgagggtaac gcgccgcgcc
    13441 attaactata aaaagatgta tgacccggat cacgagtgct atcatccgcc cttatgctta
    13501 gcgcattaac cccaggggca aataatgaac cacgatatcc cgctaaaata ttttgatatt
    13561 gccgatgagt acgcgaccga atgcgcagag cctgtcgcag aggctgaacg cacaccgctg
    13621 gcccactact tccagttact gctcacccgt ttgatgaaca atgaagagat cagcgaagaa
    13681 gcccaacatg aaatggctgc tgaagcgggg attaatcccg tacgcattga tgagatcgca
    13741 gagtttttga atcaatgggg taatgagtaa ttatttaatt gcagacgatc ggtcagtatc
    13801 gttgtccgga taaagcgttc acgccgcatc cggctaacaa tgacacgcac tgcagcgtgc
    13861 tttaatgggt ttctcacgat cgtaccgctc tgctcctcgc taagttgttc tgtgagttat
    13921 agttatagag aaatcacggg tacgaatgtt gaacagatgg cgacgccacc tgttcaacga
    13981 ccaaccacac ggccctcatt acgacccctg aacacaaacg gtaatggcaa atgtcattaa
    14041 atattagtaa gatagcctct ttacataaaa tcccttaata ttatgcgcca ggtattatcg
    14101 tctcttttgg tgattgctgg acttgtgagt ggtcaggcaa tcgccgcgcc tgaatccccc
    14161 ccgcatgctg atatccgcga cagcggtttt gtctattgcg tcagcgggca agtcaacacc
    14221 tttaacccat ccaaagcgag cagtgggtta attgtcgata cccttgccgc ccagttttat
    14281 gatcgactgc tggatgtcga tccctatacc tatcgcctga tgccggaact tgccgaaagc
    14341 tgggaagtac tcgacaacgg cgcgacctat cgcttccacc tgcgtcgcga tgttccgttt
    14401 caaaaaaccg actggtttac tcccactcgt aaaatgaatg ccgacgatgt ggtgtttacc
    14461 ttccagcgaa tttttgaccg caacaacccg tggcataacg tcaacggcag caacttcccc
    14521 tacttcgaca gcctgcaatt tgccgataac gtgaaaagcg tccgcaaact ggataatcat
    14581 accgttgagt tccgactcgc ccagccggat gcttcttttt tgtggcacct cgcaacccat
    14641 tatgcttcgg tcatgtcggc agaatatgcc cggaagttag agaaagaaga tcgccaggag
    14701 caactcgacc gtcaaccggt cggcaccggt ccgtatcagt tgtcggaata ccgcgccggg
    14761 caatttattc gcctacaacg tcatgatgac ttctggcgcg gtaaaccgtt aatgccgcag
    14821 gtagtggtgg atttaggctc cggcggcacc ggacgtctgt cgaaactcct gaccggggaa
    14881 tgcgacgttc tggcctggcc tgctgccagc cagctatcca ttttgcgtga cgacccgcgc
    14941 ttgcgtttaa cgctgcgtcc tgggatgaac gtcgcctatc tggcatttaa caccgccaaa
    15001 ccgccgctaa ataatcccgc tgtccgccat gcactggcac tggcgattaa taaccagcgc
    15061 ctgatgcaat ccatctatta tggtacggct gaaacggcgg cctctatttt accgcgcgcc
    15121 tcgtgggcct atgacaacga ggctaaaatt actgaataca atccggcgaa atcgcgcgaa
    15181 cagttgaagg cgttggggct ggaaaactta acgctgaaac tgtgggtgcc cacacgttcg
    15241 caggcgtgga accccagtcc actgaaaact gccgaactga ttcaggcgga tatggcgcag
    15301 gttggcgtaa aagtggtgat tgtgccggta gaaggtcgct ttcaggaggc gcggttgatg
    15361 gatatgagcc atgatctgac gttatccggt tgggcgacgg acagtaacga cccggacagt
    15421 ttcttccgtc cgttactgag ctgcgcggca attcattcac agaccaacct cgcccactgg
    15481 tgcgatccga aattcgacag cgtgttgcgt aaggcgctct cctcgcagca gctggcagcg
    15541 cgtattgaag cctatgacga agcgcagagt attctggcgc aggaattacc tattctgccg
    15601 ctggcgtcgt cattgcgttt gcaggcctat cggtacgata tcaaaggtct ggtacttagc
    15661 ccgtttggta acgcctcctt tgctggggtg tatcgcgaga aacaggatga ggtgaaaaaa
    15721 ccatgattat cttcacctta cgccgcattt tgttattgat tgtcaccttg ttcctgctga
    15781 ccttcgttgg cttcagcttg agctatttca ctccacacgc gccgttacaa ggcgcgtcac
    15841 tgtggaatgc ctgggtgttc tggtttaatg gcctgatcca ctgggatttt ggtgtttcca
    15901 gcattaacgg tcaaccaatt gccgaacagt taaaagagct cttcccggcg acgatagaac
    15961 tgtgcatcct cgccttcggt tttgcgctga ttgttggcat ccccgtgggg atgattgccg
    16021 ggattacgcg ccataagtgg caggataact tgatcaacgc catcgcctta ctgggctttt
    16081 caatcccggt gttctggctg gcgcttctgt tgacactgtt ttgttcactc acgctaggtt
    16141 ggttgcccgt ttcagggcgt ttcgatctgc tctacgaagt gaaaccgatt accggttttg
    16201 cgttgattga tgcctggctt tcggactcac catggcggga tgaaatgatc atgagcgcaa
    16261 tccgccatat gatattgccc gtgatcaccc tgtcggttgc gccaacaact gaagtgatcc
    16321 gcctgatgcg tatcagcact attgaagtgt atgaccagaa ctatgtaaaa gcggcggcga
    16381 cccgcggttt gtcgcgcttt actattttgc gtcgccacgt tctgcataac gcgttaccgc
    16441 cggttattcc tcgcctgggc ttacagtttt ccaccatgct gacgctggcg atgatcaccg
    16501 aaatggtttt tagctggccg ggtttagggc gctggttaat taacgccatc cgccagcagg
    16561 attacgcagc catttccgcc ggagtgatgg tgtgtggctc actggtcatt attgttaacg
    16621 tgatttctga tattttgggt gccatggcta accctctgaa acataaggaa tggtatgcct
    16681 tacgatagcg tatacagcga aaagcgcccg ccgggcacgc tgcgtaccgc ctggcgcaaa
    16741 ttttatagtg acgcctctgc gatggtcggc ctgtacggct gcgcgggact ggctgtactg
    16801 tgtatttttg gcggctggtt tgcgccttac ggcatcgacc agcaatttct cggttatcaa
    16861 ttattgccgc catcatggtc gcgctatggc gaagtttctt tcttcctggg gactgacgac
    16921 ctcggacgtg atgttttaag ccgtttactg agcggagctg cgccaaccgt gggtggcgca
    16981 tttgtggtga cgcttgccgc gacgatctgt ggcctggtac tgggcacctt cgccggggcg
    17041 acacacggcc tccgctcagc ggtgcttaac catattctgg ataccttact ggcgatccct
    17101 tcgctgctgt tggcaattat cgttgtagcg tttgccggac cgagtttgtc ccacgctatg
    17161 tttgctgtct ggctggcatt gctgccgcgt atggtacgtt cgatttacag catggtgcat
    17221 gacgaactgg aaaaagagta cgttatcgcc gcccgtctgg atggcgcatc aacgctgaat
    17281 attctctggt ttgctgtgat gccaaacatc accgccgggc tggtcaccga gatcacccgc
    17341 gcactgtcga tggcaattct cgatatcgcc gcgctgggct ttctcgatct cggcgcacaa
    17401 ctcccctcgc ctgaatgggg agcgatgctc ggtgatgcgc tggaactgat ttatgtcgcc
    17461 ccgtggactg tcatgctgcc aggtgcggca attatgatta gcgtgttgtt ggttaacctc
    17521 cttggcgatg gcgttcgtcg tgcgattatt gcgggggtgg aataatgcca ttactcgata
    17581 ttcgtaacct gaccattgaa tttaaaaccg gtgatgagtg ggttaaagcc gtcgaccggg
    17641 taagtatgac cttaaccgaa ggtgaaatcc gcggtcttgt tggtgaatcc ggttccggca
    17701 agagtttgat tgcgaaagcg atttgtgggg tgaataaaga taactggcgt gttactgctg
    17761 accgtatgcg ttttgatgat atcgatttgc tgcgtctctc cgcacgcgaa cggcgcaaac
    17821 tggttggcca taacgtgtcg atgattttcc aggaaccgca gtcgtgtctt gacccttcag
    17881 aacgtgtggg tcgccagttg atgcaaaaca tcccagcctg gacctataaa ggccgttggt
    17941 ggcagcgttt tggctggcgc aaacgccgtg ccattgaact gctgcaccgc gtggggatta
    18001 aagatcacaa agatgcgatg cgcagttttc cctatgagtt gaccgaaggt gaatgtcaga
    18061 aagtgatgat agccattgca ctggcgaatc aaccgcgtct gctgattgct gacgaaccga
    18121 caaactcaat ggagccaaca acccaggcgc aaatctttcg cctgctgacg cgtctcaacc
    18181 aaaacagcaa taccactatt ttgcttatca gccatgactt acaaatgctt agccaatggg
    18241 cggataaaat taacgtgctt tactgcggtc aaacagtgga aaccgcgccg agtaaggagt
    18301 tggtgacgat gccacatcat ccttataccc aggcgctgat ccgcgcgata ccagacttcg
    18361 gcagcgcgat gccgcataaa agtcgcctca atactctacc cggcgctatc ccattgctgg
    18421 aacagttacc gattggttgt cgtctggggc cacgttgccc gtatgcacaa cgagaatgca
    18481 ttgtgacgcc acgtttgacg ggggcgaaaa atcatctcta tgcctgtcat ttcccgctga
    18541 acatggagaa aaagtgagat gatcgaaacg ctgcttgaag tgcgtaattt aagtaaaacg
    18601 ttccgctacc ggaccggttg gtttcgtcgt cagaccgtag aagcggtaaa acccttgagc
    18661 tttacgctac gtgaaggcca gacactggcg attattggcg agaatggttc gggtaaatcc
    18721 acactggcaa aaatgctggc gggaatgata gaacctacca gcggcgagtt attgattgac
    18781 gatcatccgc tgcattttgg cgattattcc ttccgtagtc agcgtattcg catgattttt
    18841 caggatcctt cgacctcgtt gaatccccgt caacgtatct cacaaattct ggattttcca
    18901 ctgcgcctga acaccgatct ggaaccggaa cagcgtcgca aacagattat tgaaacgatg
    18961 cgtatggttg ggctgctacc ggatcacgtc agttactatc cgcatatgtt ggcacccgga
    19021 caaaaacagc gtctgggtct ggcgcgcgcg ctgatattgc gcccaaaagt cattattgcc
    19081 gatgaagccc tcgcttcact ggatatgtca atgcgttcgc agttgattaa tctgatgctg
    19141 gagttacagg aaaaacaggg catttcgtat atttatgtta cccagcatat cggaatgatg
    19201 aagcacatta gcgaccaggt gctggtgatg catcagggcg aggttgtcga gcgaggcagc
    19261 accgcggatg tgctggcatc gccgctgcat gaactcacca aacggctgat tgccgggcac
    19321 tttggcgaag cattgacagc ggatgcgtgg cgtaaagatc gctaattttc tgaatcctcc
    19381 ttccagatta ttcacctcat tctgacaaga gattaaatct tgtcagaatg aggtgatgga
    19441 taaaataaaa tcaaatgcgc gtgatttaag acgcaatctc actttgcagg aacgaaagct
    19501 ctggcgatat cttcgcagcc gacgttttgg tgatttcaaa tttcgccgtc aacatccggt
    19561 ggggagctac attctcgatt ttgcttgctg ctcggcgcgc gtagtcgttg agctggatgg
    19621 tgggcagcat gatttggcag ttgcctatga taccaggcgc actagctggc ttgagtcgca
    19681 gggctggacc gtgctgcgtt tctggaataa cgagattgat tgtaatgagg aggcggtgct
    19741 ggagattatt ctgcaggaac tgaaccgccg gtcaccctca ccctgaaagg gcgagggggc
    19801 cgaccgagcc gaatagctgt tgtggtgaaa acatggagac agtgctggag aatattcggc
    19861 aaggtctgaa ccatcccagc catcgccata aaagggttag gggctgtatg agcctgtttg
    19921 ttgctggggt aacaatattt gcacaatatg gtcccctcgc ccctctgggg agagggttag
    19981 ggtaagggga aaagcgcccc cctgccgcag cctgctccgg tcggacctgg caactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccac
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaagga gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtggcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta gtattataac gttataagaa
    26821 ttacagcgat gaattcccga caacaaacta ttctacagat ggtcattgac cagggtcagg
    26881 ttagcgtaac cgatctggca aaagccactg gagtttctga agttaccatt cgccaggatc
    26941 tcaacaccct cgaaaaactg agttacctcc gccgtgcaca tggctttgca gtttcgcttg
    27001 atagtgatga cgtcgaaacc cgtatgatga gcaactatac gctgaagcgt gaactcgccg
    27061 agtttgccgc gtcactggtt caaccgggcg aaaccatctt tatcgaaaat ggcagcagca
    27121 atgccctgct cgcccggact ctgggggagc agaagaaaaa tgtcactatc atcacggtca
    27181 gcagctacat cgcgcatttg ctgaaagacg cgccttgtga agtcattttg ctcggtggcg
    27241 tgtaccagaa aaaaagcgaa agtatggttg gccctttgac acgccagtgc atccagcagg
    27301 tacatttcag caaagcattt attggtattg atggctggca acctgaaact ggattcaccg
    27361 gtcgcgatat gatgcgcacc gatgtggtca atgccgtgct ggaaaaagag tgcgaagcga
    27421 tagtcctgac tgacagctcg aaatttggtg ctgtacattc atactccatc ggtcccgttg
    27481 agcgattcaa tcgcgtgatt accgattcga aaatacgcgc cagcgatctg atgcatcttg
    27541 agcacagtaa actcaccgtt cacgtcgttg acatttaacc atttcccttc tctacggatg
    27601 atttgcagtt tggcaaatca tccgctctaa gatgattcct ggttgataat taagactatt
    27661 tacctgttat taacactctc aagatataaa attattatca gcgatataac aggaagtaat
    27721 tatcacctgc gtgatataac cctgcgcgcg agcagatttc acggaataat ttcaccaggc
    27781 ttattcttag ctattatagt tatagagagc ttacttccgt gaatcataaa ttcaggagag
    27841 agtattatgt ttgtaacgag caaaaaaatg accgcggctg ttctggcaat tactttggca
    27901 atgtctctga gtgcctgttc taactggtct aaacgggacc gcaacaccgc aatcggcgcg
    27961 ggtgcagggg cattaggcgg tgcagtactg accgatggca gtacgttggg tacattaggt
    28021 ggtgcagctg tcggtggtgt tattggtcac caggtcggta aataatcgta aataaatgtc
    28081 gattatcgac tcgcaattat aatacgcttg attctgttca gtacgaaata actgaataat
    28141 acacacagta aaatattcga aagccacgga tatatccgtg gctttttatg ttaaccgcct
    28201 gcgagtttta ccttcatccc tttcgcttca agtagtgatt ttaataaatc acgcttatcg
    28261 ccctggattt caataactcc atctttaact gctccgccgc agccgcattt tttcttcagt
    28321 tccgctgcaa gttttgtcag ttcggcatca tcgagatcga taccggtaat caggcaaacg
    28381 cccttacctt tacgtccact ggtctgacgc tgaatacgca ccgctccatc gcctttaggg
    28441 cgtactgggg ccgctttggg ctcatcaata cgcccggttt ccgttgagta gaccagacgg
    28501 ctgttggaat cactcatcat gcactccgct gtaaagaggc gttgatcgct ttcagtgtct
    28561 gcgctggatc taccgattgc gttaccgggc gaccaatcac catataatca acaccagccg
    28621 ccaacgcctg ttctggcgtc ataatgcggc gctggtcacc agcttcactc ccctgcggac
    28681 gaatgcccgg tgtaaccagt ttgaactcct gaccgaatac ctgtttaaag cgcacagctt
    28741 cctgagcaga acacaccaca ccatcaaggt cacatttttg cgtcagtgct gccagacgtt
    28801 ctgcataatc tgcaggtgac agtgtcacgc caagatcggc caggtcgctg gcttccatgc
    28861 tggtcaacac tgtcacagca atcaaaagcg gtgcatcttt gccaaacgga accagtgcct
    28921 cacgcgctgc ggtcatcata cgcgccccac cggaggcatg aacattcacc atccacacgc
    28981 ctaagtcagc tgcagcagcg acagcgtgcg ctgcagtgtt ggggatatcg tggaatttca
    29041 ggtcaagaaa gatatcaaaa ccacgctgtt gaagttcgcg cacaaactgt ggcccaaaca
    29101 atgtaaacat ctctttaccg accttcagac gacaatcgcg tgggtcgatc ttgtcgacaa
    29161 aggacagcgc gtcatcacaa ttatgataat caagggcaac aaccacagga gaattcgtaa
    29221 cggcgcggga agaagatgaa gcagttaacg tcatgaccag accttcttga tgatgggcac
    29281 cgggagtggc gcgaaacagg taaacggcga gcattctacc cgtgaaaata aaaaattaac
    29341 agttgcgatt tttcccctag caggcttttt ctgctccgtc gttgccggag cacaatggga
    29401 catcaataac tagtatgttg taactaaagt cggatttttt taaaaattac aggccatcaa
    29461 gaccgcgaat cggtttaata gttgaccagg cacgacaaga cggacaatgc cagtagaggg
    29521 tgtatgcggt aaaaccacat ttctggcagc tataacgagg cttactacgc accttctcgc
    29581 caaccatgtc acgcagcacc atcagactct ctttggcgcg cccttcttcc gcttcattta
    29641 agtggtaatc cattagctta tggaatacac gcatggtcgg atgacgctga agctggcgtg
    29701 taatatagac ctgtgcagcc tcactaccgt cgcgcgcttc gatgatatca gcaagcatca
    29761 attcagcatc ggcaccggtg ttctcttcca ccgcgcgttg caggaattct gcccattcgg
    29821 cagttttacc caactgctga tagcaggttt gcaacatttc cagcgtttcg ctgaccagtt
    29881 ctctgtcctg ggatatgacg cgttgcaaac tttcgacggc tttggcgtat tctcctttcg
    29941 ccataaacac gcgtcccatc atgatggata cgcgggcgct gtttttatct gccgccgccc
    30001 ctttttttag caaggtcatg gcacgatcga gatcgtcgct ggccatatgc tgcagggcta
    30061 actcacagta gaaatgggca atttcgacgc gctgtttatc tttacccagc ttcaccaggc
    30121 gttcggcaac atcaattgct ttctgccact cactggtagc ctggtagatt tgtagcaact
    30181 gttgcagcgc gccaatgcgg aagtcagttt catcggtcag ctgattgaac atgtcttccg
    30241 cgcggtcgta taacccggcg gccatgtaat cacgccccag ttgttgaatc gccaacagac
    30301 gctgttcata ggtcagcgag gcgctttcca ttagggtctg atggatgcga atagcgcgat
    30361 caacttcgcc acgcgaacgg aacaggtttc cgagcgtaag gtgggcttca acggtgcctg
    30421 tatcctcttt aagcatatcg agaaacaggt ctaccgcttt atcctgttga ttactaagca
    30481 ggaagttaac ccccgctacg taatcacgcg acaagcggtt ggcttcatct tgcttgtttt
    30541 gttgcgcact tctgcggccc atataccagc cataggcagc ggctacaggc aaaagcagaa
    30601 acaacaactc cagcatagaa agttattcct tcgccgctga cgagtgcggc actacagcca
    30661 cgtcagtcgc gggtgaaagc tggttttcca gtcgctttat tttacgttca gcgcgcgcca
    30721 gggaaacacg aactcgcagc cagaacaggc cacaaatcaa ccaaccgata gcaaaccccg
    30781 cagcaaacaa taccgccagc aatgtggaaa tacggtactc cccttgcgct aacagataat
    30841 taaacgtcac ctgttgatcg ttctgcgcac ccaacgtgac cgaaatcacg aagatcgcta
    30901 acaccagtaa gaaaatgagt aaatatttca cattacttcc cgttatgtgg ttagagcgaa
    30961 taaattatgg tcaacttacc gcaatagccc tttaaagtac cattttcgcg ccgtctgcga
    31021 aatggcatcc acgcgcattg ttgatgattt aaggcctaaa ttcggaaaat caaccagcgt
    31081 taactttctt gttctcgttg tgcaatttcg cgattttctt ccgcaggtgg tgttaatggc
    31141 ccacaaattc gttgcgcaag ccacgttgcc accgccacca gcgcccacga aatcaacgta
    31201 gctaccacca gatcgcgtgg ccaatgcatc ccgagcagca ggcggcttcc catgactccc
    31261 gttgcccaga ccaacaagat agcaatggtt aacgttcgcc gacgcggcca cagcaaacca
    31321 acggccagca gtgcccaact ggcagcaaac atcgtgtgac cggaaggaaa ggcaaacccc
    31381 gtctctttct gccagtgtga acgcaaatat tgtggaatat ttttctcttc agccaactgt
    31441 tcttttacta gatttccgcg ttctgctcgc tttaaagtgt agaactcatc aaccggaata
    31501 tgatgtgttt tttccaacca gataacaaaa ggtcgtggtt cctgtacttt gtctttgatc
    31561 caggatttaa cgccttgtcc aacaaggatt gccgccgcca gaatggcaaa taatacaaag
    31621 gcagccttaa tgcgaaaacg cagacaccag agaaaccagc cgaacaaaat caaatgtgta
    31681 atgacgcccc agggctgggt gacagtttca gtaacccaaa aagccgcttt tagtagccaa
    31741 ctttgttctc caggttgcca acgccagcca gaaatccata cggctactgg catgacaagc
    31801 aatagtgcag ctcccactgc ggtacgtcgg gcaatcgaac gcatggcctc tcctttttga
    31861 taagtcccac aatcataact gaaaatgcca gttccaagaa aaattgacag atttgtgcca
    31921 ttccgtgaac gatcgacgcg tcgtgattag gtgaacccct tctcgttatg gcaaaataag
    31981 ccaatacaga accagcatta tctggagaat ttcatgcagc ttaaacgtgt ggcagaagcc
    32041 aaactgccaa ccccatgggg cgatttcctg atggtgggat ttgaagaact ggcaaccgga
    32101 cacgatcatg tcgcgctagt ctatggcgat atttccgggc ataccccggt acttgcgcgc
    32161 gtccattccg aatgtctgac cggtgacgcc ctgttcagct tgcgctgcga ttgtggcttc
    32221 cagctcgaag cggcattgac gcaaattgcc gaggaaggcc gtggtatttt gctgtatcac
    32281 cgtcaggaag gtcgtaacat tggtctgctg aataaaatcc gcgcttacgc actgcaggat
    32341 caaggttacg ataccgtaga ggctaaccac cagttaggct tcgccgctga tgagcgcgac
    32401 ttcactcttt gcgctgatat gttcaaactc cttggcgtca atgaagtccg cttgttaacc
    32461 aataacccga aaaaagtcga aattctgacc gaagcaggga ttaatattgt tgaacgcgta
    32521 ccattgattg taggtcgtaa ccccaataac gaacattatc tcgataccaa agccgagaaa
    32581 atgggccatt tgctgaacaa ataaccctct tgcatcgtgt aattcatttg cttgccggaa
    32641 gcaaaataac cggcaagcaa atacttgtta cttcaacata ttacgaatga cataatgcaa
    32701 aatgccgtcg ttctggtagt aggtcaactc cgtcgcggtg tcgatacgac aacggcaggg
    32761 tacgacttcc tggctaccat ccgcgcgcgt aagcgtcacc ggaaccgtcg cgccaggttg
    32821 tagattttgc agatcgccaa tatcaatctt ctcttccccg gttagcccta acgttttacg
    32881 cgtcacgcct tgcggaaatt ccagcggcag gatgcccatg ccaattaaat tcgaacggtg
    32941 aattcgttca aacgattcgg caatcaccac acgaattcca agcagacgag gtccttttgc
    33001 cgcccagtca cggcttgagc ctgatccata ctctttcccg gcaatcaccg ccagcggcgt
    33061 ttgctcctgc ttatagcgca tcgcagcatc ataaatagag actacgtcgc tgtcaggtaa
    33121 atgccgcgtc atcccccctt caacgccagg caccatttca ttacggatgc gaatattggc
    33181 gaaggtgccg cgcatcatca cttcatggtt accacgccgc gaaccgtagg agttaaagtc
    33241 ttttcgctca cccccccgac cttgtagata tcgacccgct gggctgtcgg gcttaatact
    33301 gcccgccgga gagatatggt cagtggtgac tgaatccccc agcattgcga ggatccgcgc
    33361 accgtgaata tcttccactg gtgctggtgt tgcctgcatt tcatcaaaga aaggcgataa
    33421 gcgaatatag gttgagtcct cctgccaacc gtaggtatcg gatcgtgtga cgttaatttc
    33481 cttccactct gctgtgcctt caaaaacttc tgcgtactct ttgcggaaca tttctgtgga
    33541 gacttgttct accgcacggg caatttcttg tgccgatggc cagatatctt tcagataaac
    33601 cggctcgcct ttgcgatcat ggccgatagg ctcagaagcc aggttgatat tcatatttcc
    33661 cgccagcgca taggcaacca ccagcggtgg cgaggccagc cagttagttt taaccagcgg
    33721 atggatacgg ccttcaaagt tacggttgcc ggacagcacc gcaccgacgg ttaaatcgcc
    33781 ttttttgatt gccgtttcga taggatcggg cagcggccca gagttaccaa tacaggtggt
    33841 acaaccgtat cccacaaggt taaaccccag ttcgtcgaga tacggtgtca gtttcgcttt
    33901 tgccagataa tcagaaacga ctttcgaacc cggtgccaac gacgctttga cccacggttg
    33961 ccgcttgagg ccaagagtta cggctttttt cgccagcaag cctgcggcca tcagcacact
    34021 tgggttagag gtgttggtgc acgaggttat cgcagcaatg accacagcgc catcaggtaa
    34081 ctgatactga tgtccgttca taacataatc gactggcagg cgatctttat gcgtggcatt
    34141 cacttccagt tcgttactgg cggcaaatgc ttttggtaca tcgggcagtg caacgcgatc
    34201 ctgtgggcgt tttggccctg ccaggctcgc ttcaacgtca ttcatatcca gttctaacat
    34261 actggtaaaa atcggttcat cacccgggtt acgccacatg ccctgcgctt tggcatattt
    34321 ttcgaccaac tcgacctgat cttcgctgcg cccgcttaaa cgcatgtaat cgagggttac
    34381 tgcatcgatt gggaagaagc cacaggtggc accatattct ggcgacatat tggcaatggt
    34441 ggcgcgatcc gccaacggta gtgaatccag accatcacca taaaattcga cgaatttccc
    34501 caccacgcca tgtttgcgca gcatttgggt aacagtgaga accaggtctg tggcggtaat
    34561 accttcacgt aattttccgg taagtttaaa gcccactacg tccgggataa gcatggaaac
    34621 cggctggcct aacatggcgg cttctgcttc gatcccaccc acgccccacc ccagcacgcc
    34681 aaggccgttg atcatagtgg tgtgcgagtc agtaccaacg attgtatccg gataagcaat
    34741 ccattcaccg tcctgcaatt cgctccacac tgctttgccg agatattcga ggttaacctg
    34801 atggcaaatg cctgtgcctg gcggcacgac gctaaaccga ctgaacgctt gctttcccca
    34861 tttcaggaac acataacgtt cgtggttgcg ctccatttcc aggcgtacgt tttcttcaaa
    34921 tgcctcatca tcaccaaaac gatcgacggt caccgagtgg tcaatgacca ggtcgaccgg
    34981 tgagagcggg ttaacctttg cagtatcgcc accgaggcgt ttaaccgctt cgcgcattgc
    35041 cgccagatca acaacggcag gtacgccggt aaagtcctgc atcagcaccc ttgccgggcg
    35101 gtaggcaatt tcacggtcag catgggcatt tttcagccat cccgccagtg cgtggatatc
    35161 ctcttcggta actgagttac catcctgcca gcgcagcagg ttttcgagca aaactttgag
    35221 tgacttgggt agacgggtga tatcgcccag tgatttagca gcaagcggca ggctgtagta
    35281 gtggtaagtt ttatctttgg cctgcaacgt gtccttactg gcttctcgta gggttgacga
    35341 catagctcct ccttaatgac agggttgcga agccctgatt ctcttcaggg tgaatttaaa
    35401 gataacacaa acatcgcgta acgatttgat aacaacccaa attgataaaa gaggagaaac
    35461 cttgtggcaa agcaaatgac aaccccgccg cagcggggtc aaggatcagt gagtaatgat
    35521 ccagaggagt tcggcccaga agagcatcga cagagaaaac gcgcccatcc aggaccaata
    35581 tttcatattt gtgttgatca taggctacac ccttaacggt tattattttg tgttgagata
    35641 actcaagctg ttattgctgc ggtgtattaa aagcccttta attcattaaa aatgggtaat
    35701 aagaatgatg ataattattg tttcggctga tcatcttcag caaacagttc tataaaggct
    35761 tgctgctgcg atgttagttg ccagtcgtct ggaataacga cggaacattc ccgtttcgtt
    35821 tctggttcat tgtcactatg ctggcataat ggtgacgact ccagttgctc ctgaccgacc
    35881 atagttatcc ccacactttc caaatcagca gtgcgacaac cacccagaaa agtgcagaac
    35941 caagaaaaac cgccagccag gctttacgtt ttaattcagg atcacggcgg ggttcctgat
    36001 ttccagaagg cattgctaac ctcatataat cgccatcact tatcagcaag acgttgaatg
    36061 attggtgtaa ttaatcatac gatgagacaa atcctaaaga aactttagtc aaaaatccag
    36121 cgaatttacg aatcgtctcc agtgaataat tcaatctttt gacctgaaat agatatttga
    36181 aaactggaat ttgcgaggcg ggtaattaga cactacttta caggtaattt accctataaa
    36241 tatgagggat aaaaatattg tgcacggtat agaaataagt aacaaaagag aatatattcc
    36301 ggcaccttcg ctacataaaa ggtgccgaaa ataacgcaag aaattatttt tccggcagtt
    36361 ttatatcttt aaacatgacc tcaatttctt cattggagcg caatgcgaca gccgcatcaa
    36421 cgacatcacg cgttaaatgc ggtgcaaaac gctgaatgaa atcatacata taactgcgca
    36481 aaaaagtact acggcgaaaa ccaattttgg ttgtactgtg gctgaagata tcgtgagcat
    36541 caacgcgcac aaggtcggga tcggcgaccg gatccaccgc catgctggca atgaccccta
    36601 cccccagccc taaccggacg taagttttaa tgacgtcagc atccgttgcc gtgaaaacga
    36661 tacgcggcgt taaccctgcg cggttaaagg cagtatccag ttctgaacgt ccggtaaagc
    36721 cgaaggtata tgtcaccaac ggatattgcg ccagttcttc aatggtaatg gcttttttgc
    36781 ctgccagcgg gtgatccgga gtgactacaa tagcccgatt ccagtggtag cacggtaaca
    36841 tcactaaatc ttcatacaga tgcagcgctt ctgtggcgat agcgaaatcg gcattgcctt
    36901 tagagacggc atcagcaatt tgtgtcggcg ggccctggtg catatgcaaa gaaacgcggg
    36961 gataacgctc aataaagcct ttgataacgt ttggtaacgc gtagcgtgcc tgggtatgcg
    37021 tggtggcgat atacagcgag cctttatccg gccaggtgtg ctcaccggca accgatttta
    37081 tggcatcgac tttcgacagg acttcgcgag cgatacgaat tatttcttgt cctgctggcg
    37141 ttacctgcgt caggtgcttg ccgcttcggg aaaaaatttg aatgcctagc tcgtcttcca
    37201 gcattctgac ttgtttactg atcccgggtt gtgatgtgta aagtccttcc gctgttgatg
    37261 agacattcag gttatgattg accacctcaa caatatagcg aagttgttgt aatttcatgt
    37321 taaaccatcc acttagacgc catcagaaca tcgttttttc gtcacacctg gcgggtgact
    37381 aatacgattt aataataaaa aaggtgctaa ctataaccac tatatcattt atagccttag
    37441 tgtataggtg tgaagaaaaa ataatatgca ataaaaaagg gccgctttcg cgaccctttg
    37501 tttataaaaa cctgacagaa ttaaaggtta tttttttcct tcaacccatt tgccatcaac
    37561 ataaaatgct gaccagccag tcgcctttcc gtctttttcc gaagagacat attgctgttt
    37621 ggttttacgg ctaaagcgaa ccatggtctt attaccttcc ggatcctgct gtggcgcatc
    37681 ggccagataa cgcagttttt ccggcagacg gtcgcggaag cgataaagct cttccaccag
    37741 tggcgcacgc gtttcacgcg atttcgggaa agtgttggca gccaggaaca caccggcagc
    37801 accgtcacgc agcacgaaat aagcatctga tttttcgcac ggcagctcag gtaatggcac
    37861 cggatcttct ttcggtggtg ccacttcgcc gttacgtaaa atcttacgtg tgtttttaca
    37921 ctcttcgttg gtgcaggcca tgtatttacc gaaacgcccc attttcaggt gcatttcaga
    37981 gccacatttt tcacactcaa cgatcgggcc gtcataacct ttaatgcgga attcgccctc
    38041 ttcgatctcg taaccgtcgc aggttgggtt attaccacag acatgcaact tacgtttcgg
    38101 atcgatgaga tagctgtcca tcgccgtgcc gcatttcggg caacgacgtt ttgcgcgcag
    38161 cgcgttggtt tcagcatctt cgccttccag cacgttcagc acttcgtttt ccggcaccag
    38221 gttaatggtg gttttgcaac gctctttcgg cggcagcgca tagccagaac agccaaggaa
    38281 taccccggta ctcgctgtgc gaatccccat tttgcgacca caagtcgggc agtcaatgct
    38341 ggtcagaacc atctggttcg ggcgcatacc accctcttcc ggatcttttt cagctttatc
    38401 taactgctgg gtgaaatccg agaagaagtg atccagtaca gctttccact ctgcttcgtg
    38461 atttgccacc tggtcgaggc tgttttccat ctgcgcggta aagtcgtagt tcattaactc
    38521 gcggaaattt tcttcaaggc gatcggtgac gatttcgccc attttttccg catagaaacg
    38581 acgattttct actcgcacat agccacgatc ctgaatggtc gaaatgatcg acgcataggt
    38641 agacggacga ccgataccac gtttttccag ttctttaacc agcgatgctt cactgaaacg
    38701 ggctggcggc ttggtaaagt gctgggctgg tgttagttca acgagcgtca gagcatcgcc
    38761 tttatcgact gccggtaaga tacgatcttc atcgccttta cgcagtgcag gcatcacttt
    38821 tgtccagcca tcaaagcgca aaatacgacc gcgtgctttc aggcggaaat cgcccgcacc
    38881 aacggtcagc gtcgtggagt catatttcgc tggggtcatc tggcaggcaa cgaactgacg
    38941 ccagattaac tggtacagtt tctgcgcatc tgcttccata tccttcagcg attccgccat
    39001 cacattgacg tcagaaggac gaatcgcttc gtgcgcttcc tgtgagtttt ctttgctggc
    39061 gtactgattc ggactttccg gcagatattt cttaccaaaa ttatcgctga tataaccgcg
    39121 aaccatattt accgcgtcct gactcaggtt agtggagtcg gtacgcatgt aagtgatata
    39181 gcctgcttca tacaaacgct gcgccatcat catggttttt ttcacgccaa aaccaagacg
    39241 ggtgctggca gcttgttgca gcgtagaggt aataaaagga gcgccaggtt tactggttgt
    39301 cggtttgtct tcacgttcca gcacgctgta gcgcgctttt tccagcagac ttaccgcagc
    39361 ctgagtttgt tctttgttga ccggacggaa cggtttgtca ttctgatgag tcacctgcaa
    39421 cgccaacgct tcaccagatg gcgtagtcgt gctggcatcg acttcccaga actcttccgg
    39481 tacgaacgct ttaatttcac gctcacgctc taccaccagg cggaccgcca ccgactgcac
    39541 acgaccggca gacagaccac gagcgatctt tttccacagc agcggcgaaa ccatataccc
    39601 caccacgcgg tccataaagc gacgcgcctg ctgggcatta acacgatcaa tattcagctc
    39661 acccggtttg ttaaatgcct ggcggatcgc gtttttagta atttcgttaa acaccactcg
    39721 gctatagcgc gcatcatcac caccaatcac ttcccgcagg tgccatgcaa tggcttcccc
    39781 ttcgcggtca aggtcggttg cgagatagat gtggtcggct ttttcagcca gttgtttcag
    39841 ttcagagacg accttctctt taccaggcaa cacttcatag tgcgcctccc aattgtgcca
    39901 cgggtcaacc cccatacggt tgacgagagc gccacgttca tcctttttag gctttttagc
    39961 cgtcttggta gaggtagagt cggcactctt tttggcagct gagccactgg tcggcaaatc
    40021 gcggatgtga ccgacgctgg atttcaccac gtagtcacaa cccagatact tgttgatcgt
    40081 tttggctttt gccggggact caacgatgac aagagcttta cccatattca ccttacctaa
    40141 tttgattctt ccaggaatgc gtcgcacgtt gataaacctt ccactggcaa cgagttaccg
    40201 atatggactc gctctctgcg gatatcaacc ccttgtcgtg actctgttca cgaaaattca
    40261 ggtgactgag ttaacaggtc ttggcctaca ggcgctatag cacgacggaa ttttggtcga
    40321 atgtcaagca aatctattgc cagattgacg aaagcgtcac actgtacctg ataaaatccg
    40381 atacgcaact ttattagcat gcaaaatcca aagttgccag taaggcgctc ttgccaccag
    40441 cgtggtatat ttgtctgcaa gccatgcctg gcgtagacta atgtccttgt ttaaggagta
    40501 aataatgaac aaagagacac aacccatcga ccgcgaaacg ctgttgaaag aggccaacaa
    40561 aatcattcgt gagcatgagg atacgctcgc agggattgaa gccaccggcg tcacgcagcg
    40621 taatggcgtg ctggtattta ccggcgacta ctttttagat gaacaaggat tgccaacagc
    40681 caaaagcacc gcggtgttta atatgttcaa acacctggcc catgtgcttt cagaaaaata
    40741 tcatctggtt gattaaagca aaacgcgagg tcttagcctc gcgtttgtct tttacatcaa
    40801 tggcttttga ccccgctgcc accagcgtag caacaatcga tcggcgctct ctgccgcgct
    40861 gccggtgaat cggtcaatga gtcgtttacg ctgcatatag cgtacattaa ccacttcacg
    40921 gccttccatc aggctaagaa taacttcatc actggtgttg atttcatcaa ccaggccttt
    40981 ctctaccgcc tgttgtccgt accagtgttc acccgttgcc acctgttcaa tatccagaga
    41041 cggacgcata cgcttcacaa aatctttaaa taactgatgc gtttcgttca gctcttcgcg
    41101 gaatttctcc cgcccttctt cggtattttc acccagcaag gtcagcgtgc gcttatactg
    41161 cccggcggtg tgcagttcga tatcaatatc tttgcttttc aggaagcggt taaagttggg
    41221 catttgcgcc accaccccaa tggaacccac aatagcaaac ggtgcggaaa caattttgtc
    41281 cgccacacag gccatcatgt aaccgccgct ggcagcgact ttgtctaccg taacagttaa
    41341 aggaatgttt ttatcacgca gacgctgcag ctgcgaagcc gccaacccgt aaccatgcac
    41401 catgccacca gggctttcca gacggagcac aacctgatcc tgcggtttga atgctgcgag
    41461 taccgccgtt atctcttcac gtagcgagtt cacttcatgg gcgtccatgc tgcctttaaa
    41521 atccagcacc cagacgcgag gtttactgtc agttgccacc tcgcccagtt tggctttcgc
    41581 ttttgctgct ttcgcttctt gcttgtgctt tttcttctgc gctttgtgcc actgtttttg
    41641 ctgatgtgag tccatcagcg ccgcggccag ttcttctttc atctccttat actgttcgct
    41701 gagattgttg acccgtaact cgccacgctg gcgtttatta cgttgagcaa cattgacaat
    41761 aatggcggca atcgccgcaa tcgctagcac aacggtaacg attttagcca aaaacaaacc
    41821 atattcagac aacaattcca cgagtcccca ccttggttaa acaacgcttt ggctcacagt
    41881 ttacaacagg catgttaagc cgtctcgcag agatgttatt gcagcgaaag tatgacaaag
    41941 tatccatttt gcggtgttat cgccccaggc tgattgaaat gcccttctgt ttcaggcata
    42001 aagccccaaa gtcataaagt acactggcag cgcgacagac aacgtcgttg aggagtagcc
    42061 atgcattacc agccaaaaca agatttactc aatgatcgca ttatcctggt gacgggagcc
    42121 agcgatggga ttggtcgtga agccgcgatg acgtatgcac gctatggtgc gacagtgatt
    42181 ctgttgggcc gtaatgaaga aaaattacgt caggtagcca gccacataaa cgaagaaact
    42241 gggcgtcagc cacagtggtt tattctcgat ttgctgacct gcacgtccga aaattgccaa
    42301 caactggcac agcgcattgt cgttaattat ccgcgtctgg atggtgtttt gcataatgcc
    42361 ggattgctcg gcgatgtttg cccaatgagc gaacaaaatc cgcaggtctg gcaggacgtc
    42421 atgcaggtca acgttaatgc cacctttatg ctcacccagg cactgcttcc tttattactc
    42481 aaatcggacg ccggttcact ggtctttact tcatcaagcg ttggacgtca gggacgagcc
    42541 aactggggtg catatgcagc gtcgaaattt gccaccgaag ggatgatgca ggtactggcc
    42601 gatgaatatc agcagcgcct gcgcgtcaac tgcattaacc caggcggtac gcgcaccgca
    42661 atgcgcgcaa gcgccttccc gaccgaagat ccacagaaac ttaaaacacc cgctgatatc
    42721 atgccgctct acctctggct gatgggcgat gacagccgcc gtaaaaccgg catgaccttt
    42781 gacgcccaac cgggccgtaa accaggaatt tcccaatgag tgatgaacgc taccaacagc
    42841 gtcagcagcg agtgaaagaa aaagtagatg ctcgtgtggc ccaggcccag gatgaacgcg
    42901 gtattatcat cgtctttacc ggcaatggaa aaggcaaaac caccgcggca tttggtacgg
    42961 caacacgcgc agttggtcac ggaaaaaaag tgggcgtcgt gcagtttatt aaaggcacct
    43021 ggcctaatgg cgaacgcaat ctgctggagc cacatggcgt tgagtttcag gtgatggcaa
    43081 cgggctttac ctgggataca caaaaccgcg agtctgatac cgccgcctgc cgcgaagtct
    43141 ggcaacatgc aaagcggatg cttgctgatt cctcactgga tatggttttg cttgatgaac
    43201 tgacgtatat ggtggcgtat gactatttgc cactggaaga agtggtgcag gcgttaaatg
    43261 aacgtccaca tcaacagacg gtgattatca cgggtcgagg ctgtcatcgg gatattcttg
    43321 aactggcgga tacggtaagt gagctgcgcc ccatcagaca tgcgtttgat gccggtgtta
    43381 aggcgcaaat agggattgat tattaatctg taaaaatgcc ggatatgacg tccggcatac
    43441 tttgattaac cgttattacg cccgccagaa cgacgactgc cgctcacctg actgtgacgt
    43501 ttcaccgcac gacgaatctg attagccttc atgcgacgac ggtctttttc tacagcgact
    43561 ttagagctgg tttctggcgg aagctccacc agttcgcgca gatagttagt ctgggcgagg
    43621 tccagctccg tccagccacc gcgtggcaga ccttttggca gtgggatatc accgtaacga
    43681 acacggatca ggcggctcac ctgcacacca accgcttccc acagacgacg aacttcacgg
    43741 ttacggcctt cggtcagagt cacgttgtac cactggttga tcccttcgcc gccgctgaac
    43801 ttgatggttt taaaagctgc cggaccatct tccagttgca cgccacgact caaatcacgc
    43861 agtttcgcgt cgtcaacctg accaaataca cgcacggcgt attcacgttc aacttcacgg
    43921 cttggatgca ttaaacggtt tgccagttca ccatcggtgg tgaacagcag cagaccacag
    43981 gtgttaacgt ccagacgacc tacggcaatc cagcgtgcgc cacgcagctt tggcagacgg
    44041 tcaaacaccg tcggacgccc ttccggatcg ttacgggtac acaactcacc ttccggtttg
    44101 taataggcca gaacgcgaca aatttgttca gcggactcac gtaccgaaat cagatgacca
    44161 tcgatacgaa ttttcagccc aggggtaact tcaacgcgat cgccgagttt agcaattttg
    44221 ccatcaacac tcacacgacc ggcttcaata atagattcga tttcacggcg ggagccgtgg
    44281 ccagcgcggg ccagcacttt ctgtaatttt tcgctcatag agcttccttt tggtgtcgcc
    44341 ttcacaggcg tcgaacagga gaatcaatgg cgccgtatag cgccatttta aggccgcgta
    44401 gtatacagag ttacgcactt ataagaaagg cttcacatca cctacgcctt cacgcaccac
    44461 gaccggcgta tcatcggtaa gatcaataac cgttgttggt ttctgcccga gatagccgcc
    44521 gtgaataatc aaatctacct gtttttccag acggtcttta atttcttcgg gatcggattc
    44581 ggtaaattcg ctgcctggca gcattagcga agtggaaagc atcggttcgc ccagtgcttc
    44641 aagcaacgcc tgcgcaatcg ggttagaagg cacacgcata ccgatggttt tgcgtttctc
    44701 ctgcaacaga cggcgtggca cttccttcgt ccccttcagg atgaaggtat agttgcccgg
    44761 cgtgttgttt ttcatcaaac gaaacgcaac gttatccacg aatgaatagg tcgagagttc
    44821 agaaagatcg cgacacatca gggtaaagtt gtgaccgtcc ggcagctggc gaatacgaca
    44881 aatacgctcc atggcgtttt tatcttcaat tttacagccg agcgcatagc cggaatcagt
    44941 tggatagaca atcaccccgc ctttacgcac gatctccacc gcctggttga tcagacgttg
    45001 ctgcgggtta tcaggatgaa tataaaaaaa ctggctcata attccctctc tgtggtgttc
    45061 tgcggctgtt cccatagctg ccagacgcct tcaacgcctg cgggcagcca gagtttacga
    45121 cccagttcga tccacgggca tggctgatga aaatcagatc cttgtgatgc ccataaatga
    45181 tgctgacgcg caagtgtggc cagctgggtg cgttcattgg gcgattgctg gcactgcgcg
    45241 acttccatcg cgtcgccgtg gtgttcggca aaatgcgcaa ccaatctttt cagccattta
    45301 gcactaagat tgtaccgccc aggatgagct agcaccgcct taccgccaga atgatgaatg
    45361 acatcaatag cttgttctat tgtacaccac tgtggcggaa cgtatccggt tttcccgcgc
    45421 gccagatact ttttaaagac atctgccatt gaactggctt tgccgcactc cacaaggaaa
    45481 cgcgcaaaat gaccgcgcgt cactgcgccg ccctgcgcca gtcgttgtgc gccttccagc
    45541 gccccaggta tttgcgcttt ttcaagtctt tcggcaattt gctgcgcccg ctgattgcgc
    45601 cgttctgtct gctgcgcgag gaactcacac attagcggat gagtaatatc aatattcagc
    45661 ccgacaatat gaatttcatg attttcccag accgtggaaa tttccacgcc gggaataaga
    45721 ttcagcgcca gtcccgaacg tgaaatttcc tctcttgctg gcgcgattgc agctgtggtg
    45781 tcatggtcgg tgatcgccag ggtgccgacg cgcatctcga ctgcacggtg caccaatgct
    45841 tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgca
    45901 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat
    45961 aacgggtctg gcaaatattc tgaaatgagc tgttgacaat taatcatcga accagttaac
    46021 tagtgcgcaa gttcacgtaa aaagggtatc gacaatgaaa gcaattttcg tactgaaagg
    46081 ttggtggcgc acttcctgaa acgggcagtg tattcaccat gcgtaaagca atcagatacc
    46141 cagcccgcct aatgagcggg cttttttttg aacaaaatta gagaataaca atgcaaacac
    46201 aaaaaccgac tctcgaactg ctaacctgcg aaggcgctta tcgcgacaac ccgactgcgc
    46261 tttttcacca gttgtgtggg aatcgtccgg caacgctgct gctggaatcc gcagatatcg
    46321 acagcaaaga tgatttaaaa agcctgctgc tgatagacag tgcgctgcgc attacagcat
    46381 taggtgacac cgtcacaatt caggcacttt ccggcaatgg cgaagccctg ctggcactac
    46441 tggataacgc cctgcctgcg ggtgtggaaa gtgaacaatc accaaactgc cgtgtgctgc
    46501 gcttcccccc tgtcagtcca ctgctggatg aagacgcccg cttatgctcc ctttcggttt
    46561 ttgacgcttt ccgtttattg cagaatctgt tgaatgtacc gaaggaagaa cgagaagcaa
    46621 tgttcttcgg cggcctgttc tcttatgacc ttgtggcggg atttgaagat ttaccgcaac
    46681 tgtcagcgga aaataactgc cctgatttct gtttttatct cgctgaaacg ctgatggtga
    46741 ttgaccatca gaaaaaaagc acccgtattc aggccagcct gtttgctccg aatgaagaag
    46801 aaaaacaacg tctcactgct cgcctgaacg aactacgtca gcaactgacc gaagccgcgc
    46861 cgccgctgcc agtggtttcc gtgccgcata tgcgttgtga atgtaatcag agcgatgaag
    46921 agttcggtgg cgtagtgcgt ttgttgcaaa aagcgattcg cgctggagaa attttccagg
    46981 tggtgccatc tcgccgtttc tctctgccct gcccgtcacc gctggcggcc tattacgtgc
    47041 tgaaaaagag taatcccagc ccgtacatgt tttttatgca ggataatgat ttcaccctat
    47101 ttggcgcgtc gccggaaagc tcgctcaagt atgatgccac cagccgccag attgagatct
    47161 acccgattgc cggaacacgc ccacgcggtc gtcgcgccga tggttcactg gacagagatc
    47221 tcgacagccg tattgaactg gaaatgcgta ctgaccataa agagctgtct gaacatctga
    47281 tgctggttga tctcgcccgt aatgatctgg cacgcatttg cacccctggc agccgctaca
    47341 tcgccgatct caccaaagtt gaccgttatt cctatgtgat gcacctcgtc tctcgcgtag
    47401 tcggcgaact gcgtcacgat cttgacgccc tgcacgctta tcgcgcctgt atgaatatgg
    47461 ggacgttaag cggtgcgccg aaagtacgcg ctatgcagtt aattgccgag gcggaaggtc
    47521 gtcgccgcgg cagctacggc ggcgcggtag gttatttcac cgcgcatggc gatctcgaca
    47581 cctgcattgt gatccgctcg gcgctggtgg aaaacggtat cgccaccgtg caagcgggtg
    47641 ctggtgtagt ccttgattct gttccgcagt cggaagccga cgaaacccgt aacaaagccc
    47701 gcgctgtact gtgcgctatt gccaccgcgc atcatgcaca ggagactttc tgatggctga
    47761 cattctgctg ctcgataata tcgactcttt tacgtacaac ctggcagatc agttgcgcag
    47821 caatggtcat aacgtggtga tttaccgcaa ccatattccg gcgcagacct taattgaacg
    47881 cctggcgacg atgagcaatc cggtactgat gctttctcct ggccccggtg tgccgagcga
    47941 agccggttgt atgccggaac tcctcacccg cttgcgtggc aagctgccca ttattggcat
    48001 ttgcctcgga catcaggcaa ttgtcgaagc ttacgggggc tatgtcggtc aggcgggcga
    48061 aattcttcac ggtaaagcgt cgagcattga acatgacggt caggcgatgt ttgccggatt
    48121 aacaaacccg ctgccggtgg cgcgttatca ctcgctggtt ggcagtaaca ttccggccgg
    48181 tttaaccatc aacgcccatt ttaatggcat ggtgatggca gtacgtcacg atgcggatcg
    48241 cgtttgtgga ttccagttcc atccggaatc cattctcacc acccagggcg ctcgcctgct
    48301 ggaacaaacg ctggcctggg cgcagcagaa actagagcca gccaacacgc tgcaaccgat
    48361 tctggaaaaa ctgtatcagg cgcagacgct tagccaacaa gaaagccacc agctgttttc
    48421 agcggtggtg cgtggcgagc tgaagccgga acaactggcg gcggcgctgg tgagcatgaa
    48481 aattcgcggt gagcacccga acgagatcgc cggggcagca accgcgctac tggaaaacgc
    48541 agcgccgttc ccgcgcccgg attatctgtt tgctgatatc gtcggtactg gcggtgacgg
    48601 cagcaacagt atcaatattt ctaccgccag tgcgtttgtc gccgcggcct gtgggctgaa
    48661 agtggcgaaa cacggtaacc gcagcgtctc cagtaaatcc ggctcgtcgg atctgctggc
    48721 ggcattcggt attaatcttg atatgaacgc caataaatcg cgccaggcgc tggacgagtt
    48781 aggcgtatgt ttcctctttg cgccgaaata tcacaccgga ttccgccacg cgatgccggt
    48841 tcgccagcaa ctgaaaaccc gcaccctgtt caatgtgctg gggccattga ttaacccggc
    48901 gcatccgccg ctggcgttaa ttggtgttta tagtccggaa ctggtgctgc cgattgccga
    48961 aaccttgcgc gtgctggggt atcaacgcgc ggcggtggtg cacagcggcg ggatggatga
    49021 agtttcatta cacgcgccga caatcgttgc cgagctgcat gacggcaaaa ttaagagcta
    49081 tcaattgacc gctgaagatt ttggcctgac tccctaccac caggagcaac tggcaggcgg
    49141 aacacctgaa gaaaaccgtg acattttaac acgcttgtta caaggtaaag gcgacgccgc
    49201 ccatgaagca gccgtcgctg cgaacgtcgc catgttaatg cgcctgcatg gccatgaaga
    49261 tctgcaagcc aatgcgcaaa ccgttcttga ggtactgcgc agtggttccg cttacgacag
    49321 agttaccgcg ctggcggcac gagggtaaat gatgcaaacc gttttagcga aaatcgtcgc
    49381 agacaaggcg atttgggtag aaacccgcaa acagcagcaa ccgctggcca gttttcagaa
    49441 tgaggttcag ccgagcacac gacattttta tgatgcgcta cagggtgcac gcacggcgtt
    49501 tattctggag tgtaaaaaag cgtcgccgtc aaaaggcgtg atccgtgatg atttcgatcc
    49561 ggcacgcatt gccgccattt ataaacatta cgcttcggca atttcggtgc tgactgatga
    49621 gaaatatttt caggggagct ttgatttcct cctcatcgtc agccaaatcg ccccgcagcc
    49681 gattttatgt aaagacttca ttatcgaccc ttaccagatc tatctggcgc gctattacca
    49741 ggccgatgcc tgcttattaa tgctttcagt actggatgac gaccaatatc gccagcttgc
    49801 agccgtcgct cacagtctgg agatgggggt gctgaccgaa gtcagtaatg aagaggaact
    49861 ggagcgcgcc attgcattag gggcaaaggt cgttggcatc aacaaccgcg atctgcgtga
    49921 tttgtcgatt gatctcaacc gtacccgcga gcttgcgccg aaactggggc acaacgtaac
    49981 ggtaatcagc gaatccggca tcaatactta cgctcaggtg cgcgagttaa gccacttcgc
    50041 taacggtttt ctgattggtt cggcgttgat ggcccatgac gatttgcacg ccgccgtgcg
    50101 ccgggtgttg ctgggtgaga ataaagtatg tggcctgacg cgtgggcaag atgctaaagc
    50161 agcttatgac gcgggcgcga tttacggtgg gttgattttt gttgcgacat caccgcgttg
    50221 cgtcaacgtt gaacaggcgc aggaagtgat ggctgcggca ccgttgcagt atgttggcgt
    50281 gttccgcaat cacgatattg ccgatgtggt ggacaaagct aaggtgttat cgctggcggc
    50341 agtgcaactg catggtaatg aagatcagct gtatatcgat acgctgcgtg aagctctgcc
    50401 agcacatgtt gccatctgga aagcattaag cgtcggtgaa accctgcccg cccgcgagtt
    50461 tcagcacgtt gataaatatg ttttagacaa cggccagggt ggaagcgggc aacgttttga
    50521 ctggtcacta ttaaatggtc aatcgcttgg caacgttctg ctggcggggg gcttaggcgc
    50581 agataactgc gtggaagcgg cacaaaccgg ctgcgccgga cttgatttta attctgctgt
    50641 agagtcgcaa ctgggcatca aagacgcacg tcttttggcc tcggttttcc agacgctgcg
    50701 cgcatattaa ggaaaggaac aatgacaaca ttacttaacc cctattttgg tgagtttggc
    50761 ggcatgtacg tgccacaaat cctgatgcct gctctgcgcc agctggaaga agcttttgtc
    50821 agcgcgcaaa aagatcctga atttcaagcc cagttcaacg acctgctgaa aaactatgcc
    50881 gggcgtccaa ccgcactgac caaatgccag aacattacag ccgggacgaa caccacgctg
    50941 tatctcaagc gtgaagattt gctgcacggc ggcgcacata aaaccaacca ggtgctgggg
    51001 caggcgttgc tggcgaagcg gatgggtaaa accgaaatca tcgccgaaac cggggccggt
    51061 cagcatggcg tggcgtcggc ccttgccagc gccctgctcg gcctgaaatg ccgtatttat
    51121 atgggtgcca aagacgttga acgccagtcg cctaacgttt ttcgtatgcg cttaatgggt
    51181 gcggaagtga tcccggtaca tagcggttcc gccacgctga aagatgcctg taatgaggcg
    51241 ctacgcgact ggtccggcag ttatgaaacc gcgcactata tgctgggcac cgcggctggc
    51301 ccgcatcctt atccgaccat tgtccgtgag tttcagcgga tgattggcga agaaaccaaa
    51361 gcgcagattc tggaaagaga aggtcgcctg ccggatgccg ttatcgcctg tgttggcggc
    51421 ggttcgaatg ccatcggcat gtttgctgat ttcatcaatg aaaccaacgt cggcctgatt
    51481 ggtgtggagc ctggtggtca cggtatcgaa actggcgagc acggcgcacc gctaaaacat
    51541 ggtcgcgtgg gcatttattt cggtatgaaa gcgccgatga tgcaaaccga agacgggcag
    51601 attgaagaat cttactccat ctccgccgga ctggatttcc cgtctgtcgg tccacaacac
    51661 gcgtatctta acagcactgg acgcgctgat tacgtgtcta ttaccgatga tgaagccctt
    51721 gaagccttca aaacgctgtg cctgcacgaa gggatcatcc cggcgctgga atcctcccac
    51781 gccctggccc atgcgctgaa aatgatgcgc gaaaacccgg ataaagagca gctactggtg
    51841 gttaaccttt ccggtcgcgg cgataaagac atcttcaccg ttcacgatat tttgaaagca
    51901 cgaggggaaa tctgatggaa cgctacgaat ctctgtttgc ccagttgaag gagcgcaaag
    51961 aaggcgcatt cgttcctttc gtcacgctcg gtgatccggg cattgagcag tcattgaaaa
    52021 ttatcgatac gctaattgaa gccggtgctg acgcgctgga gttaggtatc cccttctccg
    52081 acccactggc ggatggcccg accattcaaa acgccactct gcgcgccttt gcggcaggtg
    52141 tgactccggc acaatgtttt gaaatgctgg cactgattcg ccagaaacac ccgaccattc
    52201 ccattggcct gttgatgtat gccaatctgg tgtttaacaa aggcattgat gagttttatg
    52261 cccagtgcga gaaagtcggc gtcgattcgg tgctggttgc cgatgtgcca gttgaagagt
    52321 ccgcgccctt ccgccaggcc gcgttgcgcc ataatgtcgc acctatcttc atctgcccgc
    52381 caaatgccga tgacgacctg ctgcgccaga tagcctctta cggtcgtggt tacacatatt
    52441 tgctgtcacg agcgggcgtg accggcgcag aaaaccgcgc cgcgttaccc ctcaatcatc
    52501 tggttgcgaa gctgaaagag tacaacgctg cacctccatt gcaggggttt ggtatttccg
    52561 ccccggatca ggtaaaagca gcgattgatg caggagctgc gggcgcgatt tctggttcgg
    52621 ccattgttaa aatcatcgag caacatatta atgagccaga gaaaatgctg gcggcactga
    52681 aagtttttgt acaaccgatg aaagcggcga cgcgcagtta atcccacagc cgccagttcc
    52741 gctggcggca ttttaacttt ctttaatgaa gccggaaaaa tcctaaattc atttaatatt
    52801 tatcttttta ccgtttcgct taccccggtc gaacgtcaac ttacgtcatt tttccgccca
    52861 acagtaatat aatcaaacaa attaatcccg caacataaca ccagtaaaat caataatttt
    52921 ctctaagtca catattcctc aggtaattgt taatatatcc agaatgttcc tcaaaatata
    52981 ttttccctct atcttctcgt tgcgcttaat ttgactaatt ctcattagcg actaatttta
    53041 atgagtgtcg acacacaaca ctcatattaa tgaaacaatg caacgcaacg ggagaaataa
    53101 catggccgaa catcgtggta gttcaggaaa tttcgccgaa gaccgtgaga aggcatccga
    53161 cgcaggccgt aaaggcggtc agcatagcgg cggtaatttt aaaaatgatc cgcaacgcgc
    53221 atctgaagcg ggtaaaaaag gcggtcaaca aagcggtggt aataaatcag gcaaatcctg
    53281 attcaccgtt aattattaat atcagctgat aaagataatt gcatctgcgg gccgtatggc
    53341 tcgcagtgat ttcactggag aaaaatatga atatgaagac cattgaagat gtatttattc
    53401 aactgctttc agatacctac agcgcagaaa aacaattaac ccgggcactg gcaaaactcg
    53461 caagagcaac atcaaatgaa aaattaagtc aggcttttca tgcgcacctc gaggaaactc
    53521 atggacagat tgaacgaatt gatcaagttg tggaatcaga atcaaatctg aaaattaagc
    53581 gcatgaaatg tgtggcaatg gaaggtctta ttgaagaagc taatgaggtc atcgaaagta
    53641 ccgagaaaaa cgaagtgcgt gatgccgcac tgattgccgc agcacagaaa gtcgagcatt
    53701 atgagattgc cagttacggg acattagcga cgctggctga acaattaggt taccgtaaag
    53761 cagcgaagct tctgaaagaa accctggaag aagaaaaggc caccgacatc aaactgactg
    53821 atctggccct taataacgta aataagaaag ccgaaaataa agcctgaaat atgaatttta
    53881 acttttagtc attttataaa gaggacattt tcatgaatcg tattgaacat tatcatgact
    53941 ggttacgtga cgcccacgca atggaaaagc aagccgaatc tatgcttgaa tccatggcca
    54001 gccgtataga taattatcct gaactacgcg ctcgtattga acaacatctt agtgaaacca
    54061 aaaaccagat tgttcaactg gaaactattc ttgatcgtaa tgacatttca cgttcagtca
    54121 ttaaagattc catgagtaaa atggctgcgc ttgggcagtc aatcggtggt atattccctt
    54181 ctgatgaaat agttaaaggc tctattggcg gatatgtctt cgagcaattt gaaatcacct
    54241 gttacacctc actattagca gcagcaaaaa atgccggtga tacagtctca attccgatca
    54301 tcgaagcgat tttaaatgag gaaaagcaaa tggccgactg gctgattcag catattccgc
    54361 aaacaactga gaaattttta attcgttctg aaactgatgg cgtagaagcg aagaaataat
    54421 accctttata ccatgtcctt attgaccccg tatattacgg ggtcgttttt gtgcggaatt
    54481 aaaaacgata tcctgctgag aacataaaca cccacggatc gaggcgtacg ctatcgtgtt
    54541 gctgtgcacc gcctagctta taattggcgg tggtatcgat atccatgtac cacactgaca
    54601 tgttaaccaa ccagtcacgg ttaatcagat aatcaacccc cacctgcccg gcagctcccc
    54661 aggaatcttt cagactgaga tcggaaagcc ccgcctcttt gccatgatcg ttaaatccat
    54721 tatcaaagaa ggtggtgtag ttaataccgg ccccaacgta aggacggaat ttgctgctgg
    54781 catcaccaaa ataccactgc gccatcagtg ttggcggcag atggtgaacg gttgcaatat
    54841 cgccggtcgc ccgggtgccg attttatggc ggaacggcgt cgctgccagt aattccacac
    54901 caatgttgtc ggtcgccata taagtaaacg tcaagcccag ttgagtgtta ttggtcacgc
    54961 tgaatccacc cagacttcct aacgtaccac cagcaccttc tgttggacgt acggttgcag
    55021 aacctgcacg cataaaaaat tcgcctgctt catgcgcaaa ggcactgcca gaaagaagag
    55081 ttgttactgc caaagccgcc actgttaact ttttcatatc cgctccgtcg ttatggttat
    55141 aaaaacgtga cgaatatacc tacattggag taacaaatga tcctacatag atcacattac
    55201 atatgataat tcaccattta ttgatctgga ttaatttaga agttgcattg agaatcctgg
    55261 ttaatgtgat ttaaatcaat ttttaaatat tttctctacg caaatattaa ctcgatcgcc
    55321 acttgccgcg gtttgcccac gcctgaaggc cagcaattac ctccgcttta caaagatatg
    55381 cttttccata caagacctga tgctgccacc cgaggcttaa ccagggtaca attgcccgct
    55441 aattaacacc tgcataaaac tcaaggagag tgcatgtcta tcacggcgca gtccgtatac
    55501 cgtgacaccg gaaatttctt ccgtaatcaa tttatgacca ttctgttggt atcgttgcta
    55561 tgtgcgttta tcacagtggt gttagggcat gttttctcac ccagtgatgc acagcttgcg
    55621 cagctcaatg acggcgtgcc cgttagcggc agtagtgggt tgttcgacct ggttcagaat
    55681 atgtcaccgg aacagcaaca aattttgctg caggcttcag cggcgtccac tttttcagaa
    55741 ttaatcggta acgccattct cgccggaggc gtaatattaa ttatccagct ggtgtctgcg
    55801 ggtcagagag tcagtgcgct gcgagctatt ggtgccagtg cgccgatatt gcccaagtta
    55861 tttattctga tttttctgac taccctttta gtacagattg gcatcatgct ggtggtcgtt
    55921 ccgggaatta tcatggccat tttactggcc ctggcaccgg tgatgctggt acaggacaaa
    55981 atgggcattt ttgcctcgat gcgtagcagt atgcggctga cttgggcgaa tatgcgtctg
    56041 gtggcacccg cagtactgag ctggttgctg gcaaaaacac tgttgctgct ttttgcctct
    56101 tcttttgccg cattaacccc ggaaattggt gccgtactgg cgaacacctt gagcaacctg
    56161 atttctgcca tattgctcat ctatctgttc cgcctgtata tgttgattcg ccaataacct
    56221 taagcatttg attacggaat cgtaaaatga agcagtttct tgatttttta ccgctggttg
    56281 tctttttcgc gttttacaag atttatgaca tctatgcggc tactgcagcg ctgatcgtcg
    56341 ccacggcgat tgtgcttatc tatagctggg ttcgctttcg taaggttgag aagatggccc
    56401 tgatcacttt tgttctggtg gtcgtcttcg gtggcttgac gctgttcttc cacaatgatg
    56461 agtttattaa atggaaggtt acagtcattt atgctctgtt tgcgggagcc ctgttagtca
    56521 gccaatgggt gatgaaaaag ccgctaattc agcggatgct gagtaaagaa ctcacgctgc
    56581 cgcaaccggt atggtcgaag ctaaatctgg cctgggctgt tttctttatc ctttgcggtc
    56641 tggcaaacat ctacatcgca ttctggctgc cgcaaaatat ttgggtcaac tttaaagtct
    56701 ttggcctgac cgcccttacc ttaatcttca cattgttaag cggtatctat atctaccgcc
    56761 acatgccgca ggaagataaa tcctaaccag actgccagtc cttcggggct ggcgctgtat
    56821 ctcctctgtt ctcctcagga aaatcatagt agcatcgcgc ctgtgatttt ccttttaagt
    56881 cggttttacc atgtctacaa cacataacgt ccctcagggc gatcttgttt tacgtacttt
    56941 agccatgccc gccgatacca atgccaatgg tgacatcttt ggtggttggt taatgtcaca
    57001 aatggatatt ggcggcgcta ttctggcaaa agaaattgcc cacggtcgcg tagtgactgt
    57061 gcgggttgaa ggaatgactt tcttacggcc ggttgcggtc ggcgatgtgg tgtgctgcta
    57121 tgcacgctgt gtccagaaag ggacgacatc ggtcagcatt aatattgaag tatgggtgaa
    57181 aaaagtagcg tctgaaccaa tcgggcaacg ctataaagcg acagaagcat tatttaagta
    57241 tgtagcggtt gatcctgaag gaaaacctcg cgccttacct gttgagtaat agtcaaaagc
    57301 ctccggtcgg aggcttttga ctttctgctt actgaatttc ggtggtgccg ttaattttaa
    57361 acaggatatt caccacaatc ccactgcctg gcttacccgg ctcataacgc catctgcgca
    57421 tcgcattttt cacctcacgc tcaaacatgt tcgcaggctt ggctgagagg atttgtacgt
    57481 tatccacgcg accatccggc gtgacgtcaa atttaacttt aacctgccct tcaatgcgca
    57541 atgcctgtgc tcgtgccgga tactgcggct gattacggct taatgcgcgt ggtcctgaag
    57601 ccacactggt aaccggcttg ctggttgcag ccgttgccgt acttgatgtg gggcgcgcgg
    57661 gtgccgtatt ttcaaacggt gatgccggac gcgactctac gggtttgaca tcgcgttttg
    57721 gctgctcctg tacctttttc accggcttcg gttttggctt cggcttttca atgaccaccg
    57781 gtgcttcttt tggcggttcg gggatcggct caggttccgg ttctggctct accaccggct
    57841 ccggtggcgg ctgaacggct tgtggcggtt cgagatcagc aggcgcaacc atcgtgacag
    57901 aaattggttg cgcaggcgca ggtagttcaa taacctgatg taccgaggta tagagcagcc
    57961 ccgccacaac agcaccatga atgcagaccg aaagtaacgt cggccagggg aagcggcgag
    58021 gtaaatcaag ggttattgaa gtcataatca tttcagtaga aaaactaggc ctcgatttta
    58081 aatgcaaata gcaatcatat tcaataaggc aagacgatca ggatcagtga aaatgtcatt
    58141 tttggcgtaa ttacccttac tttatgcatg accttaacaa agacatcatc tttccgttgc
    58201 agatcttcgc cctttcaaat aacgtacttt acaactttcc cgaacaagga gttgtgcccg
    58261 tgttgtatgt tatttacgct caagacaaag ctgatagcct cgaaaaacgc ctttccgttc
    58321 gtccggcaca tttagcacgt ttacagttac tgcatgatga aggtcgtttg ttgacagctg
    58381 gtccaatgcc agcggtagac agtaacgatc caggggctgc gggatttact ggctcaaccg
    58441 ttattgctga atttgaatcg ctggaagcag ctcaggcctg ggccgatgct gacccgtacg
    58501 tagcggcagg agtctatgaa cacgtttcag tgaaaccatt taagaaagtg ttctgagt
//
LOCUS       DAJTRB010000012        58063 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTRB010000012 DAJTRB010000000
VERSION     DAJTRB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.012"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(26..280)
                     /locus_tag="OO330_000824"
     CDS             complement(26..280)
                     /locus_tag="OO330_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5881803.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="OO330_000825"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="OO330_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5881804.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="OO330_000826"
     CDS             1230..2696
                     /locus_tag="OO330_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5881805.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2814..3800
                     /gene="yeiR"
                     /locus_tag="OO330_000827"
     CDS             2814..3800
                     /gene="yeiR"
                     /locus_tag="OO330_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5881806.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="OO330_000828"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="OO330_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5881807.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="OO330_000829"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="OO330_000829"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5881808.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="OO330_000830"
     CDS             5711..7267
                     /locus_tag="OO330_000830"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5881809.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="OO330_000831"
     CDS             7349..9163
                     /locus_tag="OO330_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5881810.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGHITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HILQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="OO330_000832"
     CDS             9164..10258
                     /locus_tag="OO330_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5881811.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKISDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="OO330_000833"
     CDS             10258..11283
                     /locus_tag="OO330_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5881812.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="OO330_000834"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="OO330_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5881813.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGHCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHIVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="OO330_000835"
     CDS             complement(12878..13222)
                     /locus_tag="OO330_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881814.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO330_000836"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO330_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5881815.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO330_000837"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO330_000837"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5881816.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="OO330_000838"
     CDS             15618..17378
                     /locus_tag="OO330_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5881817.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCHE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="OO330_000839"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="OO330_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5881818.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO330_000840"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO330_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5881819.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="OO330_000841"
     CDS             19115..19342
                     /locus_tag="OO330_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5881820.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19362..21122
                     /gene="pbgA"
                     /locus_tag="OO330_000842"
     CDS             19362..21122
                     /gene="pbgA"
                     /locus_tag="OO330_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5881821.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="OO330_000843"
     tRNA            21197..21273
                     /locus_tag="OO330_000843"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO330_000844"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO330_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5881822.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="OO330_000845"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="OO330_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5881823.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO330_000846"
     CDS             complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO330_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5881824.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO330_000847"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO330_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5881825.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO330_000848"
     CDS             complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO330_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5881826.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO330_000849"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO330_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5881827.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO330_000850"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO330_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5881828.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO330_000851"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO330_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5881829.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSAMLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO330_000852"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO330_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5881830.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO330_000853"
     CDS             complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO330_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5881831.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO330_000854"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO330_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5881832.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO330_000855"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO330_000855"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5881833.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO330_000856"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO330_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5881834.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO330_000857"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO330_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5881835.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO330_000858"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO330_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5881836.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO330_000859"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO330_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5881837.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO330_000860"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO330_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5881838.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37692..38180
                     /gene="eco"
                     /locus_tag="OO330_000861"
     CDS             37692..38180
                     /gene="eco"
                     /locus_tag="OO330_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5881839.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38330..39361)
                     /locus_tag="OO330_000862"
     CDS             complement(38330..39361)
                     /locus_tag="OO330_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5881840.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39595..39954)
                     /locus_tag="OO330_000863"
                     /pseudo
     CDS             complement(39595..39954)
                     /locus_tag="OO330_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="OO330_000864"
     CDS             complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="OO330_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5881841.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41891..42541)
                     /gene="alkB"
                     /locus_tag="OO330_000865"
     CDS             complement(41891..42541)
                     /gene="alkB"
                     /locus_tag="OO330_000865"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5881842.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42541..43605)
                     /gene="ada"
                     /locus_tag="OO330_000866"
     CDS             complement(42541..43605)
                     /gene="ada"
                     /locus_tag="OO330_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5881843.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43679..44734)
                     /gene="apbE"
                     /locus_tag="OO330_000867"
     CDS             complement(43679..44734)
                     /gene="apbE"
                     /locus_tag="OO330_000867"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5881844.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44846..45967)
                     /gene="ompC"
                     /locus_tag="OO330_000868"
     CDS             complement(44846..45967)
                     /gene="ompC"
                     /locus_tag="OO330_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5881845.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46705..49377
                     /gene="rcsD"
                     /locus_tag="OO330_000869"
     CDS             46705..49377
                     /gene="rcsD"
                     /locus_tag="OO330_000869"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5881846.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTTVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49394..50044
                     /gene="rcsB"
                     /locus_tag="OO330_000870"
     CDS             49394..50044
                     /gene="rcsB"
                     /locus_tag="OO330_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5881847.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50244..53093)
                     /gene="rcsC"
                     /locus_tag="OO330_000871"
     CDS             complement(50244..53093)
                     /gene="rcsC"
                     /locus_tag="OO330_000871"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5881848.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53368..54144)
                     /locus_tag="OO330_000872"
     CDS             complement(53368..54144)
                     /locus_tag="OO330_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5881849.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54149..55798)
                     /locus_tag="OO330_000873"
     CDS             complement(54149..55798)
                     /locus_tag="OO330_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5881850.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55799..56358)
                     /locus_tag="OO330_000874"
                     /pseudo
     CDS             complement(55799..56358)
                     /locus_tag="OO330_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56517..>58063)
                     /locus_tag="OO330_000875"
     CDS             complement(56517..>58063)
                     /locus_tag="OO330_000875"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5881851.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14201 a        15662 c        14266 g        13934 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcagcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggcctggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcacccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg catataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 catcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc actgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct gggtatcgat acctggtggt atgacgttaa
     9121 taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cctcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcagcg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcactgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacattgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcggcgcac tgatgaccat
    13861 ccacgcagcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgacaacc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccacgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctaaccat cgttgttgac
    17701 ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcaaat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccagtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc agcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctagtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga cgccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgctttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg tccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaaccagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taataaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgaaggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcagcaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tgggatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaagt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc tagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcatggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 ggtttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtaactc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccagggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tagcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg actgttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaagtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc aggccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcggacgga taccgttact ggctttgcgc cagcaaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atggttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg aagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc cggcatacca tgccggatgt ggcacatcat tacaacgcaa
    38341 tatccgccac ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg
    38401 ctgcttgcgg tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
    38461 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg
    38521 tagcctgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagt agattcaaca
    38581 taatcggcgc ggcggttgac gcccctggcg atgcccccag cagcgccgca atggtccctt
    38641 gctggtcact gaccacttcg gtacccagac gcagtacgcc acctttctcg gcatcacgct
    38701 tgataatctg cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg
    38761 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
    38821 gatatttcac cagatcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
    38881 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
    38941 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacacgggta tcgatatgcg
    39001 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aactttcgcc agatggcgat
    39061 taaccacgtc tggattttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
    39121 ctttcgcttc cggaatcccc gattcctgta acagcttcag cgccgcaccg cccgcgccga
    39181 taaagacaaa tttggcgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa
    39241 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
    39301 agaagttaga tttcttctgt aaggaagcaa ttaactggcg ggtgatctca ccatagttca
    39361 catcagtgcc aatttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
    39421 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
    39481 ggcgtaacgg gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg
    39541 aacggtattg ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca
    39601 gaactggcgg gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
    39661 gccatcggcg ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc
    39721 gttgttctag ccgttcgaac tctcctgcgc gcccccctcc aggcgctcca ccatggtcat
    39781 cgaccattca ggctccagct cgcgtcaata ggtccccaac gtggcgctca taatgccgcc
    39841 gccaatcaac agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga
    39901 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
    39961 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca acggtaacac
    40021 ttaagtaaca acatttaaat aatgtttata aattatattc taattttaga aacattataa
    40081 tttcgttgat taattatagg gttattagaa atgaagggat ttttagcata ttgacctgac
    40141 ggcagcagtc cgccaggtca gcgtcgtaat attatgccgt ccgggcaacg gcatcacgcg
    40201 aagcggcatc gcgctcttcg cccgtcagct cgctaagttg cccattgcgc atttccagca
    40261 ggcggtcggc gtggataaag taatgatcat catgactgat agcgaaaata gttttaccca
    40321 tctcctgcat cagcggcagc aacacctgat aaaactcacg acggaagtgt ggatcctgat
    40381 ccaccgccca ttcatccagc aggataatat cgcgttcttc tgccagcgcc agcaacagcg
    40441 ccacgcgttt tttctgccct tttgataact tcaggttaac aatacgcccg ttgcttaact
    40501 caagcttatg agccattttc agctgcgcca gccacttctc aaccagttgc gggttagcgg
    40561 gttgcccctc cggccccagc agttgatcaa acagccagac atcggtaaac actgccgaaa
    40621 acagtttgcg ataatcttcc ggttgttcgc cgctgacagg tttgccatcc agcaagattt
    40681 cgccgctttg tggctgatac aagcccgtca acaacatcgc cagcgtcgat tttccgctac
    40741 cgttaccgcc aatcagaaac agcagctcgc cacgtttgat ggtgagatta atcggaccaa
    40801 cggaaaacgc gttatcctga taagcaaacg tcacgttacg cagctccagc gtttgccagt
    40861 tgggaaacgc ctgcgggcgc ggaaactcag ctttgaaagg cgcgagcgcg aatttgttca
    40921 gcttgttaaa cgccacctgc gccgtcagca gcgtcggcaa tgcgccaacc gccgaaagca
    40981 gcggcgtacg caggaataac agcgtcaacg aataggtcgc agcaacgttg gtatcagccc
    41041 aaccgagact gttcgccatc cagaacacca ggccgatagc gcccagcatc atgatgtttg
    41101 accagttcac ggcactaaga tggaaggtgt ctgcgcgaat aatatgatgg cgatactctt
    41161 gcgcatcggg gatgtagagg ttgttaaaca catactcggc gcgttcccgg ttcagagtca
    41221 gctctttgcg cccttcaagt acagtttgaa aatccgtgta cagcttgtct tcggtttcac
    41281 gtagggtcgc catatgtttg tacacccgcg ccaccagcac aaaaccgccc cagatggtga
    41341 tcgccatcca gatagccgtt accagtaaca ttttgcccga cagcatccac agatacgccg
    41401 atgaaccgat agtgagaatg atcccctgcc ccagttccgg cagacgcaca aaagcaatgg
    41461 tgatattgcg cacatcgctg gttaatcccg ccagcaacga ggcgctaccg agttgttcaa
    41521 tgcgctcgac gtgggtatcc agaatccgct tgataaattc actacgcagt cggtaaacga
    41581 agtgatgccc caaagtggtg agcgccagtt gcgatccgag agtgactgcc atcaacagca
    41641 gcaataatcc cagaaactcc ggtaacacca gcagactggt atccgccgtt tcgataaggc
    41701 gctgattgat aaaagcaatt aagccaatgc ctaatgccgc actggcgagg cttagcgcca
    41761 tcacactgat aaatggccag cgatactgcc gccagacaag tacaagaagt tccatgcaga
    41821 aaacccggac aatgaattac agcccgcagt ttaaacatct tcgcgcgcac agcaataata
    41881 attcttattt ttattctttt ttacctgcct gacggaatgt caggttgtag cggcagtcgg
    41941 tggtgagtgg atgaaacccc gctttcaacg gttgaatacc gtgataaaac agccgcgatt
    42001 caccgcccca taccaccaca tcgccatgtt ccaacaacaa acgtttgagc ggatcattac
    42061 gtttcaggcc accaaattga aaaatcgcgg gtaagcccag agaaacagaa acaattggcg
    42121 cgcgcagatc cggttcgtct ttatcctgat gcagcgacag tttcgcgcca ggagcgtagc
    42181 ggttgataag acaagcatct ggctggaaat ctggatagcc cgccgccgta gccgcacgtt
    42241 gacataaatc atgaaagctt tgtggcatgg cgggccacgg tttatttgtt tgcggatcaa
    42301 tgggcgaata gagataacct tgccgatggg tcgtccagcc cagatgccca cagttggtca
    42361 tcgccaccga catggtatat ccgcctggag cgaccatctg gcgaaacggc gactggctgg
    42421 caacgtcatt aatatcgcgg attagttgct ccgcagcatt aaaagcaaaa cgccgtaaaa
    42481 ttaccgcacc agccgccagt ggctcttgcc acggttcagc atcggcaaac agatccaaca
    42541 ttacctctcc tcattttcag cttcgcggcg cagcagttgc gctttacgcg acacgcccca
    42601 gcggtaaccg gaaagtgtgc catcaccacg gaccactcga tgacagggta ttacgatagc
    42661 cagcttgttg gcggcacagg cgctggcaac ggcccgtacc gctttcggtt tgccgatggc
    42721 gttagccagt tgctgataac tgacggtttc accacaaggt atcgtgcgca gtgcctgcca
    42781 gacttgttgc tgaaaagcag tgccgcgaat atccagcggt aacgtcagcg gcgtatcgcg
    42841 ttgattgagg ctggcgatca cttcacgcac atgttgctga aacgtcagat cggcaggcgc
    42901 gttgtcggca gcgggaaaca tctgctgcaa ctcgctgatt aatgtcgcgt catcatcgcc
    42961 cagcaatatc gcgcaaatcc cccgctcgct ttctgccacc aggcaacgac ccagctcaca
    43021 atcagccagc gcgtaacgca ccgccagatt ttcgccaccg tgacggaatt gtttagccgt
    43081 catgcccagc gtttcatcgg ctttgcggta gtaactgctg ctgtcgggga atccggcgtt
    43141 aagaatagac gtcgtcacgc tctccccttt cgccagcgat tcgcgcaaac ggcgagcgcg
    43201 ccaggcctgt tgccaggctt taggcgtcat tccggtagta gctttaaaca accgatgtag
    43261 atgaaatgga ctcatcgcca cctggtcggc taaggcttcc agcgttacag gcgtttcctg
    43321 ttccagcagt cgacacgcgt gggtgatttt atccaaccga tgttgccggg gattggcttt
    43381 gtctggctga caacgtttgc aggggcgaaa tccggcggcg agtgcctcgc tggcatttgc
    43441 gtagaaggag acattttccc gcaaggcatg tctggcgcgg caagaaggac ggcaaaagat
    43501 acccgtggtg cgcacggcga aaacgaattc gccgtcggca ttcgggtcgc gggctaagac
    43561 agattgccag cgttgatcgt cagttaagca tgtggcgttt ttcataatca gctccctggt
    43621 taaggatagc ctttagtctg cccggtcacc atcacgcaaa aaccaacaat cttgcgcttt
    43681 aatttttttc gctgacaagg aagcttttaa actgtggtga catccaggtt ttaaagctat
    43741 cgccttcttt ggtgatcata taaaccgcca gcccttcccg gcgaacaacc tctttggctt
    43801 tctccggccc gagtaccatc aagccagtat cccaggcatc ggcttccagc gccgtcggag
    43861 caatcaccgt cacggatacc agattgtgtt cgatgggacg cccggtttgc ggatcgataa
    43921 catgggaaag acgtttgccg tccagttcgt aatagttacg gtagctgcca gaggtgctaa
    43981 tcccatgacc gttgatatcc accacagcct gaaccgcgtt ttctttatcg gttggttttt
    44041 gaatcgctac ccgccacggc tggccttcac cgttcatacc acggctgttc agcgcgccgc
    44101 ccaccgacac cagatagcgg gaaatccctt cctgctccat caagcgtgcc aggtgatccg
    44161 ccgcataacc ttcaccgacg gtggagagat cgacatataa atccggcagg tctttttgca
    44221 gatattgctg atgcgactga ttgatgaccg tcaggtgctg taagccggtt ttggctttca
    44281 tcgcatcgat ctgttcctgg ctcggaattt gaaccggctg ttgttccggg ccaaagcccc
    44341 acagattcac cagcggcccg acggtaatat ccatcgcacc atcggtcctt gccccaatgc
    44401 gcagcgaggt ggtgacgata tcggccatcg cttcacttac cggccacggc gacaaacttt
    44461 gcgagtcgtt aaagcgcatc agcgcggaat cttttttata ggtcgaaagc agctgatcgt
    44521 cggcgtccag ctgggtctga atcttttctt taagttcggc actgcgtttg gcatcaatgc
    44581 ccgggatgct ggcacgccag aaggtaccca tggttttgcc ttcaagaacg gtaacttcag
    44641 tagcgtgggt tttggcgggt tgtggttttt gatcgcaacc aacaaagaag agcgcggcaa
    44701 ccagcagtgc cacgcgggta aagcttattt ccatacgtga ttatcctcat gcgaacggtc
    44761 gcaagagtac accaaaaaac tgtgtttgta cgctgaaaac aatgaaaaaa gggcccgcag
    44821 gccctttgtt cgatatccat cgagattaga actggtaaac cagacccaga gctacgatgt
    44881 tatcagtgtt gatgccagcg tcacgagtga actggttgtc gtccagcagg ttgattttgt
    44941 agtcaacgta ggtggacatg tttttgttga agtagtaggt cgcaccaaca tcaacatatt
    45001 tcaggatatc ttcgtcgtcg tagttacgac cattgatgac acccaggttt ttacctttag
    45061 actgcaggta agccagggac ggacgcagac cgaagtcgaa ctggtactga gcaacagctt
    45121 cgaagttctg tgctttgttc gcccaaccca agttgcctac gcgagttgcg ttgtaggtct
    45181 gggtgtactg agcagctagg tagatgttgt tcgcgtcgta tttcagacca ccggtgtagg
    45241 tttcagcgcg gtcgccatta ccgatgtaac gctcatcata acggttcaga ccgaagttct
    45301 ggtcatcagt acgtttggag ctggaaactg cagcaccgat accgaagcct tcgtaatcat
    45361 aagtaataga accgccaacg ccgtcaccgt tctgacgcag agcttcacga ccgttgttgg
    45421 tcatgccttc accttccggg ctaccgtttt tgccctgata ctgaacagcg aagttcagac
    45481 cgtcaaccag accaaagaag tcggtgctac ggtaggttgc gaagccgtta ccacgctgct
    45541 gcatgaagtt gtcagaaccg taagtgtcac caccgaattc tggcagtacg tcggtccagg
    45601 aagttacgtc gtaaactacg ccgtagttac gaccgtagtc gaaagaacct acatcctgga
    45661 atttcagacc tgcgaatgcc acacgggtcc aggagttgtt ttcgttttca gcgctgttgc
    45721 cctggatctg atattcccac tggccgtaac cggtcagctg gtcagtaacc tgagtttcac
    45781 ctttgaagcc aagacgcatg taggtctggt cgccatctac agacttgtcg tcagagaaat
    45841 agtgcaggcc gtctacttta ccgtacagat ctaatttgtt gccgtctttg ttgtaaactt
    45901 cagcagcgtt tgctgcgcct gctaccagca gagctgggac caggagggac agtactttaa
    45961 ctttcatgtt attaaccctc tgttatatgc ctttatttgc ttttttatgc cactgcatac
    46021 tgattaaccc tcattaatca gtcggcaagt ccattctccc caaaaatgca gaataatcca
    46081 acacgaatat gatactaaaa cttttaagat gtttcattta tcgctataga tgtttcaaaa
    46141 tgtaaataca agggaacttt ttaagattat tgcggaatgg cgaaataagc acctaacatc
    46201 aagcaataat aattcaaggt taaaatcaat aacttattct taagtatttg acagcactga
    46261 atgtcaaaac aaaaccttca ctcgcaacta gaataactcc cgctatcatc attaacttta
    46321 tttattaccg tcattcattt ctgaatgtct gtttacccct atttcaaccg gatgcctcgc
    46381 attcggtttt tttacccttc tttacacact tttcattatt ctgtgctacc acagaaaaac
    46441 tataacgctt gttaactatt tcacaaataa ttaacatcag cataatttcc agcaatcttt
    46501 gtttatttgc aattaatttc gttgggcttt ttgtaggtta tttgtacagc aaaatggcgc
    46561 ttgtacatct atttcccccc atacaggatg ataaatatca cgggagaata gagaatcatc
    46621 aatcaggtaa gagtctggaa tttcacactg taccctttat actgccctat cacttcgcga
    46681 agttttaaca ggtcataaac acgaatgcgt cagaaagaga caacggccac gacccgcttt
    46741 tcactcctac cggggagcat tacccgcttc tttttactgt tgatcattgt gttactggtg
    46801 acgatggggg taatggtaca aagtgccgtt aacgcttggc tgaaagataa aagttaccag
    46861 attgtcgaca ttacccacgc aatccaaaag cgcgtcgata cctggcgtta cgtgaccagg
    46921 cagatctacg acaacattgc cgcgacgacc tccccctcct ccggcgaagg tttacaagag
    46981 acgcgcctga aacaggatgt ctactatctg gagaaaccac gccgcaaaac ggaagcgtta
    47041 atctttggct ctcacgacaa ctcaacgctt gagatgactc agcggatgtc cacttatctg
    47101 gatacattgt ggggtgcaga aaatgtaccg tggtcgatgt attacctgaa tggtcaggat
    47161 aacagtctgg tgctgatctc aaccctaccc cttaaagatc tcacctccgg atttaaagaa
    47221 tcgaccgtca gtgacattgt tgattcacgt cgtgcagaaa tgttacaaca ggccaacgcc
    47281 ctcgatgaac gcgaaagttt ttctaacatg cgccgcctag cctggcagaa cggtcattac
    47341 tttaccttgc gtaccacgtt caaccagccg ggacatctgg caacggtcgt ggcttttgat
    47401 ctgccgatta atgatttgat cccaccgggt atgccgctgg acagtttccg ccttgagcca
    47461 gacgcgacgg caacgggaaa caatgataat gagaaagaag ggacggatag cgtcagtatc
    47521 cactttaaca gtacgaagat tgaaatctcc tcggcactca actctaccga tatgcgcctg
    47581 gtctggcagg ttccttatgg caccttattg ctggatacgt tgcaaaacat tctgctgcca
    47641 ctgctgctga acatcggttt gctggcgctg gcgttatttg gctataccac attccgccat
    47701 ttctccagcc gcagtacaga aagcgtcccc agcacgacgg tcaataacga attgcgcatt
    47761 ttacgggcaa tcaatgaaga gatagtctca ctgttgccgc tcggcctgct ggttcacgat
    47821 caggaatcga accgcactgt cataagtaac aaaattgccg atcatttgct gccgcatctg
    47881 aatctgcaaa acatcaccac catggcggaa cagcatcagg ggattattca ggcgacgatc
    47941 aataacgagc tgtatgagat ccgcatgttc cgcagccagg tcgcgccgcg cacacaaatt
    48001 ttcattattc gcgatcagga tcgcgaagtg ctggtaaaca agaaactcaa gcaggcgcag
    48061 cgtctgtatg agaaaaacca gcaggggcgg atgaccttta tgaaaaacat tggcgatgcg
    48121 ctgaaagaac ccgcacagtc cctggcggag agtgcggcta aactcaacgc cccggaaagc
    48181 aaacaactgg cgaatcaggc agatgtgctg gtgcggctgg tcgaagaaat acagttagcg
    48241 aacatgcttg cggatgatag ctggaaaagt gagacggtgc tgttctccgt gcaggattta
    48301 attgatgaag ttgtgccttc agtgttgcct gccatcaagc gtaaaggtct gcaactgctg
    48361 attaacaatc atctgaaagc acacgatatg cgccgcggcg atcgcgatgc cttacgacgt
    48421 attttgctgc tactgatgca atatgccgtg acctcaacgc aattgggaaa aatcaccctt
    48481 gaggttgatc aggatgagtc ctctgaagac cgcctgacat tccgcattct ggacacggga
    48541 gaaggcgtaa gtattcatga aatggataat ttgcacttcc cgtttatcaa ccagacccaa
    48601 aacgatcgtt atggaaaggc ggacccgctg gcattctggc tgagcgatca actggcacgt
    48661 aaactgggcg gtcatttaaa catcaaaacg cgggatgggc ttggtacacg ctactctgtg
    48721 catatcaaaa tgctcgccgc tgacccggaa gttgaagagg aagaagagcg tttactggat
    48781 gatgtctgcg taatggtgga tgttacttcg gcagaaattc ggaatattgt cactcgcctg
    48841 ttagaaaatt ggggtgcaac ctgtatcaca cccgatgaaa gattaattag tcaagattat
    48901 gatatctttt taacggataa tccgtctaat cttactgcct ctggcttgct tttaagcgat
    48961 gatgagtctg gcgtacgaga aattgggcct ggtcaattgt gcgtcaactt caatatgagc
    49021 aacgctatgc aggaagcggt cttacaatta attgaagtgc aactggcaca ggaagaggtg
    49081 acagaatcgc ctctgggcgg agatgaaaat gcgcaactcc atgccagcgg ctattatgcg
    49141 ctctttgtag acacagtacc ggatgatgtt aagaggctgt atactgaagc agcaaccagt
    49201 gactttgctg cgttagccca aacggctcat cgtcttaaag gcgtatttgc catgctaaat
    49261 ctggtacccg gcaagcagtt atgtgaaacg ctggaacatc tgattcgtga gaaggatgtt
    49321 ccaggaatag aaaaatacat cagcgacatt gacagttatg tcaagagctt gctgtagcaa
    49381 ggtagcctat tacatgaaca atatgaacgt aattattgcc gatgaccatc cgatagtctt
    49441 gttcggtatt cgcaaatcac ttgagcaaat tgagtgggtg aatgttgtcg gcgaatttga
    49501 agactctaca gcactgatca acaacctgcc gaaactggat gcgcatgtgt tgattaccga
    49561 tctctccatg cctggcgata agtacggcga tggcattacc ttaatcaagt acatcaagcg
    49621 ccatttccca agcctgtcga tcattgttct gactatgaac aacaacccgg cgattcttag
    49681 tgcggtattg gatctggata tcgaagggat cgtgctgaaa caaggtgcac cgaccgatct
    49741 gccgaaagct ctcgccgcgc tgcagaaagg gaagaaattt accccggaaa gcgtttctcg
    49801 cctgttggaa aaaatcagtg ctggtggtta cggtgacaag cgtctctcgc caaaagagag
    49861 tgaagttctg cgcctgtttg cggaaggctt cctagtgacc gagatcgcta aaaagctgaa
    49921 ccgcagtatt aaaaccatca gtagccagaa gaaatctgcg atgatgaagc tgggcgtcga
    49981 gaacgatatc gccctgctga attatctctc ttctgtgacc ttaagtccgg cagataaaga
    50041 ctaatcatct gtaggccaga taagacgcgt tagtgtctta tctggcattt gcaccgattg
    50101 ccggatgcgg cgtaaacgcc ttatccggcc tacgattccc attatttcaa cagattacat
    50161 taaagtaggc cagataagac gcgtcagtgt cttatctggc atttgcactg aatgccggat
    50221 gcggcgtaaa cgccttatcc gtcctacgaa tcccgcgatt tcctgaccct ctcggcatat
    50281 accgtcagcg tctgttttat cacatccagc gttaccggct tcgagaggca gctgtccata
    50341 ccggactcca gacaccgctg cttctcttca gccaacgcat tagcagttac tccgattacc
    50401 ggcaacgtca gtcccaactg acgaatgcgt tgcgtcaagc ggtaaccatc catatttggc
    50461 atgttgacgt cgctaagcac gatatcaata tgattcttac taagtacatt aagcgcatcg
    50521 acgccatcat tcgcggtttt acattgatag cccaacgatc ccaactgatc tgccagcaga
    50581 cgtcggttaa tcggatgatc atccacgacc agaatcatca tatcgtcgtt atcgctgacc
    50641 gctttgtccg tcgacggcag agcgttagca ggatcgtcgc tctccatctc gatcaaataa
    50701 atacgcgcca acaatgccgg tagctcatgc ggagcagcca cactgtgtac ccactcccct
    50761 ggcgctttct ccagcggaat accaatatgg cgacgacaga aggtcactac cgctctgccc
    50821 tgccattttt tactcactac ctcgtcagtg attaacacat cttcgggagt cggttcctgc
    50881 ccttcgtatg ttgtaacgac gatgccgctg cgctgcaaac tggtttccag gaactgacag
    50941 agcgacgcat tgcggaccgc cagccagcag cgtttaccac tcaacccttc cacgcctttt
    51001 ttctgcgggt actgagcgcc gtacaacgga atacgcacgg taaactggct gcccattccc
    51061 ggttctgaat ctaccgagat atcgccgtcc atcatgctga tcagtttttc acaaatcgcc
    51121 agacccagac cggtcccctg gaagtttcgc tgtacgcccg ttccaacctg gaagaaggga
    51181 tcaaacaagc gcaccacttc tttcgccgga atccccacgc cggtatcgcg aacacggata
    51241 gagagataat cgccatcagc gcgaacatgc aaaactatac agccggtatc ggtgaatttt
    51301 atggcgttac tcaacaggtt ggagatgacc tgctgtaaac gcatcgggtc gccatttaag
    51361 gccactggca catccggttc aataaagcag tacaagccta actgcttgcg taccaccagc
    51421 ggtaaatagt tggcggtgat gtggttcatc acttcacgcg gtgaaaactc acgcggttcg
    51481 atcttcaact gttccgattc aatcttcgag aaatcgagaa tatcgctgat aattttcaac
    51541 aacaggctgg aagagttgtt cattgccgtc accagacgat cgacgccttt cggtaactct
    51601 ttggtttgca acagatccag gttaccgata atgccataca gcggcgttcg cagctcatga
    51661 ctgacggtgg caaggaacat cgattttgac tggctcgcct gttccgctgc ttgtgccatc
    51721 tcctgcaacg actcttccat cttcacgcgc gaagaaacat ccaccagcac acaaatggcc
    51781 acgttttcat tacgatagcg cgaatggacg aagctgattt gcagattggt attgttgctg
    51841 gtcaggacat caacaaaatt gacctgctgc ccacagataa tttgcgtcag tcgttggcgg
    51901 tcctcatgcg taagcatatt gagataggta tgcgccagtt cgttacttaa aatattgacg
    51961 ccatcagcgg tacgcaaaat gcagataccc actggcgcgg aggcgacaat cttgcgattg
    52021 aactgctcat gttcttccag tcgcagggcg tcgctttccg ccggaatgaa aatacgtcgc
    52081 tcgtacatcc gcgcgagagt aaacaatgca gctccggcaa gcacattcag caaaattgcg
    52141 ttaaggatca acatgcgaat gcgttccagc accttatcaa ccggcaccga atacacgatg
    52201 cttagcgatg agggtggcag atttttcttc agcaccagct cccggaaccc ttccgtatag
    52261 ccaaaccagg agcgttcctg catccagcga ggatcgccct taattttact ttctggtccg
    52321 gtaagcgaaa tcagggtatg accattttca tcaagaatgg taacccccat cggcaacgta
    52381 cccggtaaga aaaagttctc catccggatg gtctgctcga cacccaaaag cgcctgcaac
    52441 cggttcgcca gataaactgg cgtcaacgcg taaaaatacc cgacgcaagg gcgcggacct
    52501 tcgctgatcc agtagaggtt actgccgcta tcatcttgtg gtgcatttcg atatttattg
    52561 atgcgttcat gcaacgcttt caacgcggta tcgcgttcca ctggcatatc acgcagaccg
    52621 aaattggcca tgcagaggtt atcactgccg attaaaaata cccggttgag atcgtaagcc
    52681 gcagaaaaat tatcgcgcca gtagcgcata aaccacgcca atgactccag agaacctcgc
    52741 caggtgttac tcattgcgga acaatcggag tcggcaaaca gcggttcaaa cgcaggcaca
    52801 tccgcctgcg tttctcgtcc acgcggggaa agcacaccgt tttctgccga taagcgattt
    52861 tcggcgatgt acttaagctc tttcatcaca tcagaggtgc gttgaataaa gcgctgagcc
    52921 tgatcggaac tcagattaaa ttcctgacga atttccgatt ctcgctgatg taacgcatta
    52981 acgatgtaaa aaacggatga aaaagcaatc aacagccaga gcactaacgc caatgctctg
    53041 aacatgtagc gcgaggcttt cagggttgta cgaaaagaag caaggtattt caaaggggag
    53101 aagctccgcc tcaggtgacc gatggagtgt ggttaaggta gcggtaaaag cgtgttaccg
    53161 caatgttctg tcaccattgc aataatgcgt atcgcttaga aaccattgcc ttaaccagga
    53221 aagccggagt agatggacag ttctcagtac gaaaaataag aaatgaccaa tctgccgacg
    53281 cttgttaatg gttatatcct ggcgtcggca gatcgcccag cagtcttatc tgaaaaaaga
    53341 gagagccagg ttgtgtgaag ggatggatca ccagtcaaaa cttttcacca gcgtcagctc
    53401 gccagcatta cgcatcggta caataaatgt ttcctgtttc tcattgaccg atccttcatc
    53461 ggtgatcagc gtcagttggg cggtcgtcaa ttccgtttcg ctgcgcccac catagtagtt
    53521 gatatacacc tgatagcgcc cgtgaactgg tgctggcatg gcgaaaatct cgggtccgta
    53581 ccccgtcgtg acatccatat ccagtgcacc actgtttttc agcacggtgt taccgtacca
    53641 ggcgtgttcg ccatcgggcg tgacaacatg gagatcgaga tcggtattgt ccgtatccca
    53701 cgagagaacc agccgtaaac gtgcacgaat agttcctgta cccggcgttg agtaaaactg
    53761 cattttttgc cggctttgcc catccgggct gatgacctgc acgctgttgc tgccttcagt
    53821 gaaaatataa gggcgtgcaa atgaaccatc ggattcaatt ctctgcggca tactggcacc
    53881 gttgaccacc agccggccct gctgaacttt acccgccgca gcataattct ttattttgcc
    53941 gcggatctgc gctgagatat tttgatcatc tgccatgttg acggacgacg ccgggtaatt
    54001 gatcgattgt gaaaaactgg catcttcgcc ctctgccgga tgccagccag agagcggtgc
    54061 gtcaatctcg acttcctgca caccttcact gtgagcgaca ggcgaaagtg ccaccagtaa
    54121 caacggaaga aaaatctttc gcatagcatt agtccattaa taattgtcgg gtcagcgttt
    54181 cgatatagtt ctcatcaagc ccggtgggat aaccatcaaa ggccaagtgc agatactcat
    54241 gggttaaatc cagccgatcc tgaagcgtaa agaagttacg aataaacagc cgtttctgct
    54301 gccgatcggt ataggggaaa cctgagacta aacgacagac cgcaaacacg tctggttcgt
    54361 tgtaccccgt ctcgccttgt aatatacgcc gccactgcgg catttttttc gccagccagg
    54421 ctttcgcttt gggtaataac tggcaggttg agcgtggagc tccccagcgg ctaaggctgt
    54481 tgtcgggata agcaaacgcg agtatctggt cgtaacgctc cccctgcccc gcctgggccg
    54541 tggcttgccg ccaggaaagt gtcccttcgg tagcgcgact gccatgataa tgaaccggat
    54601 cgcctgcata gatgagctcc tgcgtccagg cggtcatcgt tcgcgccccc gtggtcgctg
    54661 gcgaagcaga aacacgctgc gtggcgctgc tgtcggggat ggtcaggcaa tcaccttccc
    54721 gattggcgtt ttgctgtaaa aaagtacgta tcgccacagt catggctttc gctgcctcag
    54781 gtggcgtact tttcgcctcg cgatccagaa ctctggtaac gtactcttca cgatcgagat
    54841 gcgactgcaa tttcagtttg cccttttcgc tcagtaaggt cgtctcaccg tggctgacaa
    54901 acgtaatgtg gttaccatta gtaaacgtca cacgatagcg cccattaagt acgctgggtt
    54961 ttactgccgt cgtactcttt tccgctgtga ttttcttcag cggatagcgg gcaaacagct
    55021 ccacctctac acattgccca ctggcaacct gcgtcggcac tggcaaaacc cgattaagca
    55081 ccgtcgcata acgggttagt accgttttgc tggtgccact gccagtaacc cacagcggtg
    55141 taccatcggt taaccagcca gcaaaaccac cctgacggat ttcctgttta tcatcggcaa
    55201 accagctcca ggttttcacc cgtacccgac taccgagcat ggatgcgaca ccgattttcg
    55261 cctcgtccag caccacatcc agtaagactt cctgagcttt gttttgcgca ggtaacgtcg
    55321 ctaatgaatc aagcagcgat ttaaccgtta ccgaggtttc tggcttcagc gttgtaagag
    55381 acgctaacca cgcaggtgcc tgacgctgtt gccagtattg cccccacaca tctgcgccaa
    55441 tatgcaaccg ctgcggcgca aaatagagcc cacaggaacg taccagcgcg gtatcgcgcg
    55501 ttatgctctc cccagcctga caacaataaa cctcttccgg ggagtttccg cgacactggt
    55561 agggctgctc cggttgatgg gtatcctcca gccaggcgta aacatacagt ttccacaaac
    55621 tccctaacgg cgtggtgagc gagtcaggta atgctgagac tttggtcacg cctgatgacg
    55681 acagctgata cagttgatcg tgctgcgcac cgcgcagcac cagttgtaac ggcgtctcct
    55741 ccgcaagcgt tggcagagta accaacgcca atagccagac gattctgcgc cagttcactt
    55801 atttcacctg catcccggtc cattcactcc ctgccgcgac actttgctgc gcaggtgcat
    55861 aggaacgcac ataacgagca ggaggcagaa cgaattgccc tttttgcgag aagcgcagca
    55921 aatggcggaa agtgaccgtt cccgtcagtt ctttcaccgg caccatatag gccagttcgc
    55981 ccatttcatt acgcgctttt tccagcaatt gcccctgctg tttcgcggcg ttgggtttat
    56041 tgactgaaat gccccatgtt gtgcgctcaa cgtcggctcc cggcgggagc ggtacttcca
    56101 cctgaccata gcgcagaact gcatcctgct cgctggtaag cgtaatttca tcgagataca
    56161 gcgcatcgct gtcaatctca ttgctggtca ccggctgcag agtaaagctc atctcttctt
    56221 caccggggat aagccgatac aactggcgtt caacggtcac cggaatgtta ctttgttgag
    56281 ccgtttttgc cggttcacgc cagcggacct gcacatttgc ggcgataatt cgtcaccaaa
    56341 agagagaatg tccggcacac cctaaccaac ccaacgccag tcttcgcccc ctccagttaa
    56401 cttatgttta gcccatgcgc ccgcaggcgc aggcaaaaca actggaggca ttgtcgccat
    56461 atatttcgcc agccagttca tggccagcgc gcgctcaata gtggattgct cagcggtcaa
    56521 accattaaaa tagcggccgt ctgggtagcg tcaccaccgc tattgagcag cacaaccgtg
    56581 tgcgccagcg gctgattgct gtttgccgct ttatcccacg catattgctg aacctgtggc
    56641 atcaccgtgg acttcaactg cgcttttttc agcaacgtca tggttaagac gtttgccacc
    56701 gcatctgcca gtggagattc cggtgtatcg aggatcaggc tgtcattgat atcgcggatg
    56761 tcttcctcag agaaatcttc atctttagtt ccgcgccggg cgatagcttc atccccccct
    56821 ttcaacaacg ttttgcacgg cagattcatc tcctgtgccc acgccagcac cagcgcccga
    56881 tgcaataacg gcatgttatc cgcctgctcg gcataactgt cgagcatatg ctgccagtat
    56941 tccggttgtt gcgttacgcc gagcgcctgg ctggcctgcc agtcggcgta ccatgcccat
    57001 gccgtaagga aggcgtcacc attgccatct tcaccccacc aggtaaagcg cgctccgggc
    57061 cccgccagtt gcatcagccg cagacggtta tcctgaatca tctgacgaat gtcgttagcg
    57121 gcggcacttt gatgatcggc aagcgaacgc caggcgagac tgagcgggat cagacggcta
    57181 ccggtgttga tcaccccacc ccacggttca tcgactaacg catcaagatt gttgcggaaa
    57241 atctcctgcg gcgtttcact actttgtagc cggatattac tcgcctgctc gggcaacatc
    57301 agcgcgttct cgccaccgcc gagcatgaca tttttctgct gttcaacggg ccagctgttg
    57361 tccacaaaag acagttttgt gctaatgctg tcctgcactt gcccattttg ttgcagttct
    57421 gcacttaaca agccagattg ctgaatattc tgcgtcagcg aaatataatt cgcccctttg
    57481 tgcagcgtca gcgtctggcg catctcagcg cctgcaaatt tagtcaccag cgccaccggt
    57541 tcgttatcct gctgactgaa gataaacagt cctgccgccg gtttgtcgcc cacgcgatac
    57601 accgttggca tactccactt catgtagaga tttttttccg aacgcagata agcacgcccc
    57661 tgcccgacca gcccgtcgcc gttcatccca cgcgcggtga tacgccagcg ggttaacgaa
    57721 tcaggcatca ggaacgtgaa atacgctttg ccttgtttat cggttgtgag tgacggcatc
    57781 catgccgcgg tatccacctc ttcacgccgt ggacgttcaa gcatttttac tcgccgctcg
    57841 ctgcggttag tcgcgccagg cgcaaccggc tcgctggaga gcgcctggtc atagctgata
    57901 aacgacagac tggagctggt acgcacattg ttacgcccca gcggatagaa aaatttgccg
    57961 atattcggcg cgatttctgg ttgcagcgcg tagatcattt catcgaccac gcctaccgtt
    58021 agctgcgcag aaacaggttt acctttcagc gacgaggtta att
//
LOCUS       DAJTRB010000013        55921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTRB010000013 DAJTRB010000000
VERSION     DAJTRB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.013"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..745)
                     /locus_tag="OO330_000876"
                     /pseudo
     CDS             complement(1..745)
                     /locus_tag="OO330_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(720..1520)
                     /gene="otsB"
                     /locus_tag="OO330_000877"
     CDS             complement(720..1520)
                     /gene="otsB"
                     /locus_tag="OO330_000877"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5881852.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1687..2673)
                     /gene="araH"
                     /locus_tag="OO330_000878"
     CDS             complement(1687..2673)
                     /gene="araH"
                     /locus_tag="OO330_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5881853.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2688..4202)
                     /gene="araG"
                     /locus_tag="OO330_000879"
     CDS             complement(2688..4202)
                     /gene="araG"
                     /locus_tag="OO330_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5881854.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4272..5261)
                     /gene="araF"
                     /locus_tag="OO330_000880"
                     /pseudo
     CDS             complement(4272..5261)
                     /gene="araF"
                     /locus_tag="OO330_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            6058..6561
                     /locus_tag="OO330_000881"
     CDS             6058..6561
                     /locus_tag="OO330_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5881855.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6641..6892)
                     /locus_tag="OO330_000882"
     CDS             complement(6641..6892)
                     /locus_tag="OO330_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5881856.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7007..7093)
                     /gene="azuC"
                     /locus_tag="OO330_000883"
     CDS             complement(7007..7093)
                     /gene="azuC"
                     /locus_tag="OO330_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5881857.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7356..7679
                     /locus_tag="OO330_000884"
     CDS             7356..7679
                     /locus_tag="OO330_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5881858.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7850..8347
                     /gene="ftnA"
                     /locus_tag="OO330_000885"
     CDS             7850..8347
                     /gene="ftnA"
                     /locus_tag="OO330_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5881859.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8384..8623)
                     /locus_tag="OO330_000886"
     CDS             complement(8384..8623)
                     /locus_tag="OO330_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5881860.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8813..10024
                     /gene="tyrP"
                     /locus_tag="OO330_000887"
     CDS             8813..10024
                     /gene="tyrP"
                     /locus_tag="OO330_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5881861.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(10086..10751)
                     /gene="yecA"
                     /locus_tag="OO330_000888"
     CDS             complement(10086..10751)
                     /gene="yecA"
                     /locus_tag="OO330_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5881862.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10947..11033)
                     /locus_tag="OO330_000889"
     tRNA            complement(10947..11033)
                     /locus_tag="OO330_000889"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10997..10999),aa:Leu,seq:taa)
     gene            complement(11046..11119)
                     /locus_tag="OO330_000890"
     tRNA            complement(11046..11119)
                     /locus_tag="OO330_000890"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11085..11087),aa:Cys,seq:gca)
     gene            complement(11174..11249)
                     /locus_tag="OO330_000891"
     tRNA            complement(11174..11249)
                     /locus_tag="OO330_000891"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11214..11216),aa:Gly,seq:gcc)
     gene            complement(11401..11949)
                     /gene="pgsA"
                     /locus_tag="OO330_000892"
     CDS             complement(11401..11949)
                     /gene="pgsA"
                     /locus_tag="OO330_000892"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5881863.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(12006..13838)
                     /gene="uvrC"
                     /locus_tag="OO330_000893"
     CDS             complement(12006..13838)
                     /gene="uvrC"
                     /locus_tag="OO330_000893"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5881864.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13835..14491)
                     /gene="uvrY"
                     /locus_tag="OO330_000894"
     CDS             complement(13835..14491)
                     /gene="uvrY"
                     /locus_tag="OO330_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5881865.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14787..14984
                     /locus_tag="OO330_000895"
     CDS             14787..14984
                     /locus_tag="OO330_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881866.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYAWFFYCRE"
     gene            14950..15174
                     /locus_tag="OO330_000896"
     CDS             14950..15174
                     /locus_tag="OO330_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5881867.1"
                     /translation="MSTPGFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(15207..15827)
                     /gene="sdiA"
                     /locus_tag="OO330_000897"
                     /pseudo
     CDS             complement(15207..15827)
                     /gene="sdiA"
                     /locus_tag="OO330_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(16057..16809)
                     /gene="yecC"
                     /locus_tag="OO330_000898"
     CDS             complement(16057..16809)
                     /gene="yecC"
                     /locus_tag="OO330_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5881868.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16806..17474)
                     /gene="tcyL"
                     /locus_tag="OO330_000899"
     CDS             complement(16806..17474)
                     /gene="tcyL"
                     /locus_tag="OO330_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5881869.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17489..18475)
                     /gene="dcyD"
                     /locus_tag="OO330_000900"
     CDS             complement(17489..18475)
                     /gene="dcyD"
                     /locus_tag="OO330_000900"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5881870.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18580..19380)
                     /gene="tcyJ"
                     /locus_tag="OO330_000901"
     CDS             complement(18580..19380)
                     /gene="tcyJ"
                     /locus_tag="OO330_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5881871.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19468..20019)
                     /gene="fliZ"
                     /locus_tag="OO330_000902"
     CDS             complement(19468..20019)
                     /gene="fliZ"
                     /locus_tag="OO330_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5881872.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(20065..20784)
                     /gene="fliA"
                     /locus_tag="OO330_000903"
     CDS             complement(20065..20784)
                     /gene="fliA"
                     /locus_tag="OO330_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5881873.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(20949..22601)
                     /gene="fliC"
                     /locus_tag="OO330_000904"
     CDS             complement(20949..22601)
                     /gene="fliC"
                     /locus_tag="OO330_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5881874.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22867..24273
                     /gene="fliD"
                     /locus_tag="OO330_000905"
     CDS             22867..24273
                     /gene="fliD"
                     /locus_tag="OO330_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5881875.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            24298..24708
                     /gene="fliS"
                     /locus_tag="OO330_000906"
     CDS             24298..24708
                     /gene="fliS"
                     /locus_tag="OO330_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5881876.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24708..25073
                     /gene="fliT"
                     /locus_tag="OO330_000907"
     CDS             24708..25073
                     /gene="fliT"
                     /locus_tag="OO330_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5881877.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            25151..26638
                     /gene="amyA"
                     /locus_tag="OO330_000908"
     CDS             25151..26638
                     /gene="amyA"
                     /locus_tag="OO330_000908"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5881878.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26672..27085)
                     /locus_tag="OO330_000909"
     CDS             complement(26672..27085)
                     /locus_tag="OO330_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5881879.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            27272..28477
                     /gene="yedE"
                     /locus_tag="OO330_000910"
     CDS             27272..28477
                     /gene="yedE"
                     /locus_tag="OO330_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5881880.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28474..28707
                     /gene="yedF"
                     /locus_tag="OO330_000911"
     CDS             28474..28707
                     /gene="yedF"
                     /locus_tag="OO330_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5881881.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28814..29484
                     /locus_tag="OO330_000912"
                     /pseudo
     CDS             28814..29484
                     /locus_tag="OO330_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29524..30732)
                     /locus_tag="OO330_000913"
                     /pseudo
     CDS             complement(29524..30732)
                     /locus_tag="OO330_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30800..31415
                     /gene="tnpA"
                     /locus_tag="OO330_000914"
                     /pseudo
     CDS             30800..31415
                     /gene="tnpA"
                     /locus_tag="OO330_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            31743..32825
                     /locus_tag="OO330_000915"
     CDS             31743..32825
                     /locus_tag="OO330_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5881882.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(33314..34112)
                     /locus_tag="OO330_000916"
                     /pseudo
     CDS             complement(33314..34112)
                     /locus_tag="OO330_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(34122..34673)
                     /gene="ybcL"
                     /locus_tag="OO330_000917"
     CDS             complement(34122..34673)
                     /gene="ybcL"
                     /locus_tag="OO330_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5881883.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(34842..35174)
                     /gene="emrE"
                     /locus_tag="OO330_000918"
     CDS             complement(34842..35174)
                     /gene="emrE"
                     /locus_tag="OO330_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5881884.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(35519..35833)
                     /gene="fliE"
                     /locus_tag="OO330_000919"
     CDS             complement(35519..35833)
                     /gene="fliE"
                     /locus_tag="OO330_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5881885.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            36047..37712
                     /gene="fliF"
                     /locus_tag="OO330_000920"
                     /pseudo
     CDS             36047..37712
                     /gene="fliF"
                     /locus_tag="OO330_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            37705..38700
                     /gene="fliG"
                     /locus_tag="OO330_000921"
                     /pseudo
     CDS             37705..38700
                     /gene="fliG"
                     /locus_tag="OO330_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
     gene            38693..39379
                     /gene="fliH"
                     /locus_tag="OO330_000922"
     CDS             38693..39379
                     /gene="fliH"
                     /locus_tag="OO330_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5881886.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            39379..40752
                     /gene="fliI"
                     /locus_tag="OO330_000923"
     CDS             39379..40752
                     /gene="fliI"
                     /locus_tag="OO330_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5881887.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            40774..41115
                     /gene="fliJ"
                     /locus_tag="OO330_000924"
                     /pseudo
     CDS             40774..41115
                     /gene="fliJ"
                     /locus_tag="OO330_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            41112..42239
                     /gene="fliK"
                     /locus_tag="OO330_000925"
     CDS             41112..42239
                     /gene="fliK"
                     /locus_tag="OO330_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5881888.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSVPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            42344..42808
                     /gene="fliL"
                     /locus_tag="OO330_000926"
     CDS             42344..42808
                     /gene="fliL"
                     /locus_tag="OO330_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5881889.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            42813..43817
                     /gene="fliM"
                     /locus_tag="OO330_000927"
     CDS             42813..43817
                     /gene="fliM"
                     /locus_tag="OO330_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5881890.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            43814..44227
                     /gene="fliN"
                     /locus_tag="OO330_000928"
     CDS             43814..44227
                     /gene="fliN"
                     /locus_tag="OO330_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5881891.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            44230..44595
                     /gene="fliO"
                     /locus_tag="OO330_000929"
     CDS             44230..44595
                     /gene="fliO"
                     /locus_tag="OO330_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5881892.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            44595..45332
                     /gene="fliP"
                     /locus_tag="OO330_000930"
     CDS             44595..45332
                     /gene="fliP"
                     /locus_tag="OO330_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5881893.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            45342..45611
                     /gene="fliQ"
                     /locus_tag="OO330_000931"
     CDS             45342..45611
                     /gene="fliQ"
                     /locus_tag="OO330_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5881894.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            45620..46405
                     /gene="fliR"
                     /locus_tag="OO330_000932"
     CDS             45620..46405
                     /gene="fliR"
                     /locus_tag="OO330_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5881895.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            46690..47313
                     /gene="rcsA"
                     /locus_tag="OO330_000933"
     CDS             46690..47313
                     /gene="rcsA"
                     /locus_tag="OO330_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5881896.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(47357..47545)
                     /gene="dsrB"
                     /locus_tag="OO330_000934"
     CDS             complement(47357..47545)
                     /gene="dsrB"
                     /locus_tag="OO330_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5881897.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            47708..47935
                     /gene="yodD"
                     /locus_tag="OO330_000935"
     CDS             47708..47935
                     /gene="yodD"
                     /locus_tag="OO330_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5881898.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            48233..49048
                     /locus_tag="OO330_000936"
                     /pseudo
     CDS             48233..49048
                     /locus_tag="OO330_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(49045..50739)
                     /gene="yedQ"
                     /locus_tag="OO330_000937"
                     /pseudo
     CDS             complement(49045..50739)
                     /gene="yedQ"
                     /locus_tag="OO330_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(50910..51092)
                     /locus_tag="OO330_000938"
     CDS             complement(50910..51092)
                     /locus_tag="OO330_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5881899.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(51171..52088)
                     /locus_tag="OO330_000939"
     CDS             complement(51171..52088)
                     /locus_tag="OO330_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5881900.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            52261..53181
                     /gene="yedA"
                     /locus_tag="OO330_000940"
     CDS             52261..53181
                     /gene="yedA"
                     /locus_tag="OO330_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5881901.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTSKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(53170..53640)
                     /gene="vsr"
                     /locus_tag="OO330_000941"
     CDS             complement(53170..53640)
                     /gene="vsr"
                     /locus_tag="OO330_000941"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5881902.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(53621..55039)
                     /gene="dcm"
                     /locus_tag="OO330_000942"
     CDS             complement(53621..55039)
                     /gene="dcm"
                     /locus_tag="OO330_000942"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5881903.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(55106..55646)
                     /locus_tag="OO330_000943"
                     /pseudo
     CDS             complement(55106..55646)
                     /locus_tag="OO330_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(55686..>55921)
                     /locus_tag="OO330_000944"
     CDS             complement(55686..>55921)
                     /locus_tag="OO330_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881904.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT        13981 a        14386 c        13533 g        14021 t
ORIGIN      
        1 aattccgctt taagttgcgc cagttttggc ggcagtggcc cggcagcctg tttggctatt
       61 tctttcggtt caatgccgat cgggtagact tctgttcgaa atgcttttcc ccaggctgta
      121 tggctttttg cgctacgtgt cgtgacgcgg gtcaggttag aaaggcaatc caggaacgcc
      181 agacgatcgt tttctgtctg taaacccagc aaatcataat cacaaagctg ttcaagcaag
      241 gtgtcatatg tcggcagcgc gttgaaaatt tccggtgtcg ggaaaggaat atgcaagaaa
      301 aaaccaatgc gattattcac cccacgtttg cgtaattcat gcgcaaaggg caacaggtga
      361 taatcgtgga tccagataat gtcatcgtct tgcaacagcg gcagtaattt atctgccagc
      421 aacgcattta cgcgtagata gccgtcccag gcaggacgct gaaattgcac cagatcgagc
      481 cgataatgaa aagcgggcca gagaacggca ttggagaatt ggttgtagta ttcgtcaagg
      541 tcctgttcgc tgaggttaaa agaggcccac gtaatgttac cttttttagc ggctgatcct
      601 cattccctgt ttcaccactc cagccaaacc acagtccgcc tgcggctttc agtgccccca
      661 gtatgccaac ggcaaggcca ccggcactgg cggcgtgctc gtctggtggt gcaatccggt
      721 tagatactac gactaaacga ctcatagtca tcactcctgt tattttctct tttttgttgt
      781 aatgcggcgg ttatcatttc aagccagctc cagacatccg gcacacccgc cagtcgccat
      841 gatgcctgag ttgcacctgt gccaattttt actgacattc cgcccagtcg gttaacgact
      901 gcgaagccag attcatcggt taaatcatcg cccagaaata cgggcgttcg cccgataaaa
      961 ggagcttcct gcataaaggc tgcaatcgct tcacctttac tggtacctct cggtttgatc
     1021 tcgacaacac actttccctg ctgtaacgcc atttgtggcc agatctgagt aatacgttgc
     1081 gctaatgtca ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag cgcaaaagcc
     1141 atccctttcg cctccagctc cgcgccggga tactgagcga tgaccgtatg cagttgcacg
     1201 ctaatatcac gcgcaatcgc atccggcaga tgaacgatat gtgttttacc attgatgtca
     1261 cggcgctccg ccccatgcac gcccgctaac gggaagcgat aaggttttgc cagtgcgtca
     1321 agctccacca ttgagcgccc tgatatcaat gccaatgcac catcacttgc ggttgccagt
     1381 agctgtagtc cttgcagaat attgtcaggc acgacgacct gatcgggatg cggtttgatt
     1441 tccgccagcg ttccatcaag atcaaaaaac caggcatatt tcgcggatag ttcaggggtt
     1501 tcggttaacg gttctgtcac ccggctctcc tccttctctt ttcttattgt gttgcgcaga
     1561 ccgcgcttta cagacatgta agtatagaca gtgtgacgga ggtcgccatt tgcaacgaag
     1621 aacagccaac cgttgtggcg gctggctgtg gtgggaaaaa acgttaaatt gttgtggaaa
     1681 aaagcatcag acagtgcgct tcgctttttg cttgtaacgg tcgaagatca ccgctgccag
     1741 caggattaag ccgcgaacca cgtactgcgc gaaaggagaa atattaagca ggttcatggc
     1801 gttttccacg gtgcctaaaa ttaagatacc cgccaccaca tatgagattt ttccgatgcc
     1861 acctttcaga gaaacgccac ctaaaacgca ggcggagata acaataagct cataaccaat
     1921 cgacgtcatt ggctgcccac tagtcatacg tgaagccaga ataattccgg ctatcgcaga
     1981 taccaggcca gagagaacaa agataataat tttggtgcga acaaccggta cacccgccag
     2041 acgcgcagcc tcttcgttcc cgccaatcgc cagggtatta cgaccaaagg tggttttatt
     2101 cagcagcaaa ccaaagataa tcagacacgc gacggtgagc cagattggcg caggcagacc
     2161 gaaccagtta gcgtaaccaa gggcaaagaa gctttcatct tcgataccga ccgctttacc
     2221 gtctgaaatg atgtacgcca gacctcgaac aatctgcatc gttgccagtg tcgtgatcag
     2281 agcatttatt ttcagtttgg cgataacaaa gccattgacc aggccacaga gaacgcccag
     2341 caacaaaccc gctgccacgc caatccacag gctttcagtc aggttgataa ccaccgccgt
     2401 ggtgacaccc gcacaggcaa ttacggaggc gacagaaagg tcaaagtcac cggaagcaag
     2461 gcagaacaac atgccacacg ccaccatccc cgacatggaa attgccaggc ccaacccttt
     2521 catattaatg aaggtggcaa aatttgggac aaaaatggca caggcgataa agagcaccgc
     2581 aaacaccacc agcatgccgt actgatccca gatacgcccg aagctgaatg acgacttagg
     2641 tgcgccagac cccgatgtag aaacagaaga catcatactc tccttactca ggcaactgcc
     2701 tggctgactt taggcatcgc aaggctcagt gcctgacgct catctgcctg ctcgtgtaac
     2761 aattcaccgg cgatttcgcc ttcccgcatc accacaatcc ggtcggcaac gccgaggact
     2821 tcaggtaagt cgctggaggc aaacagcacc gccacgccct gcgccgccag cgcataaatt
     2881 acgttatata tttcgtgctt agcgccaaca tcaatgccgc gcgtaggttc atccagcaaa
     2941 atgaccttca tctcttccga taaccagcgg cccagaatgg ctttttgctg atttccgcct
     3001 gagagattca tgatcagttg ctccgcgccc ggcgttttga tgttgagcga acgaatgtgc
     3061 tgatcggcat tgttttcttc ccaactgttg ttgattacac aaccgccgag cacatgtttg
     3121 cgtctggcac tgatgttgat attgtcgcga acggagtgca cgggaataat gccttccgct
     3181 ttgcgagctt ccgggcagag catcatgcct gcggcaatgg cgtggctcgg tttacgaata
     3241 tcgatcggct gttggtcgat ataaacctga ccggcggtga tttgcgtccc gccaaacaag
     3301 cctttcatta attcgctacg ccccgcccct accagaccaa acagcccaac aatttcacca
     3361 ctgcgaaccg ccagacttat tggcgtacgc acgcctagtg ctttcacagc atcaagacgc
     3421 aggcgctcct cgccataact acgaggttgc cagccgtaga tatcgccaat gtcgcgcccg
     3481 accatcgcct gcaccagcgc gtcgtggtca acctgctgca tatcggtaaa ggttttgaca
     3541 taacgtccat ctttaaagac agtgatggca tcgctgaggg caaatatttc ttccatacgg
     3601 tgagaaacgt ataagatcac ccgcccctct tttcgcagtt cacgaataac gcggaaaaga
     3661 ttgccgattt cgcgggcaga aagggagctg gttggctcat caaaggcgat aattttagcg
     3721 ttacgcgcca gcgctttggc gatttcaacc atctgccact gaccaatggc gagatatttc
     3781 agcggcgtgt ccgggtcaat atccatacca agatgtttaa gttgtaaacc cgcctcataa
     3841 ttcagcaatg agcgattcac aatgccgcct ttatgcggca gctggccgag atagatgttt
     3901 tccgcgacgg tcatttccgg cacgagatgc agttcctggt aaataatcgc cacacccgcg
     3961 ttaagtgctg cggtcgtgtc ggaaaaggac atttcctgcc cattaatcac tacagaaccc
     4021 gtggttggcg catagttgcc gctgaggatt tttaagagag ttgattttcc tgcgccattt
     4081 tcacccatca acgcatgaac ctgaccggca tagcagtcaa aactaatatc cgtcagcgcc
     4141 ttaacgccgg gaaacgtttt accgatgccg cgaaatgaga gatacggggt agactgttgc
     4201 ataacgtctc cgtgaatcac tggtcgttca caccccctct gcatcatgca gaggggaatt
     4261 tttccggcaa attacttacc gcctaaacct tttttctcca gttcttcttt aaagttgtca
     4321 cgcgtgatca gtaccacgtc tgtaacttcg gtaaattttg gcgtttcaac gtcttttgct
     4381 acccagttgt aaagcatttc gctggattta tagccatgta cgtccgggct tggcagcagg
     4441 gaaccgtaga agccggttgc ctgtgcttta gacagttcgc ttactgcatc cacaccgtta
     4501 ataccaatac cgatgatatc ggctgcttta aagccctgac cttcggttgc acgtacgccg
     4561 ccaagtacgg tgctgtcgtt cataccgacg atcagccaat gtttaacttc cggatgttga
     4621 accagcattg agttggcagc gtcaaatgcc cccgggatgt cgttagattt ggtaggtacc
     4681 tgataaattt gtttttccgg gaatcctgca gctttcagtg cgtccataga acccgttgta
     4741 cggcggcggg cggtatccag ttcgttggcg gtaatcgcca tcactgcgct ttctttgaca
     4801 tcccagccac gtttctgcat ctctttatac agttcctagc cctgacgttc gccaatttta
     4861 gtcgccgcca tcatcaccag aggaacggta tccattggct tacctttggc gttaacaaac
     4921 tggtcatcca ctgcaatgac tttcatatcg tagccacgcg ctttcgcgac gatggcagag
     4981 ccgagtttcg ggtccggagt acaaataacg aaaccttttg cgccactggc tgccaggctg
     5041 tcgatcgcgt tcaatgtttt ttcgccatcc ggcacggcaa tcttaataac ctcaaaccct
     5101 aaatccttcc cggctttatc ggcaaacttc cattcggtct ggaaccacgg ctcttccggt
     5161 tgcttcacca gaaaaccgag cttcaggttc tccgccatag cggattgtga cataacggct
     5221 gccagaccaa tggctgccag ggctttagta aatttgtgca tggttctctc cagctttagt
     5281 gtcgttttgt gtagtgcaaa aacgaatgac attcgttaaa ttaatcggaa aacaaagcat
     5341 taccttttat ctaaaagata agtgaccgtg ttgacatagt tttagcgaga aattaattct
     5401 ctataggaga gcaatatcac atcgcagaat tacagtgaga acgtgcataa atttagcggg
     5461 aaaagacata aggaaaagcc aatttgtcag acaaattgtt gaatgcacag cagattaatc
     5521 cataagatta gcctggaaat ccttgttgtc ttcggtaccc atgcgggatg tcttcttttt
     5581 aaccagtcaa taggccgcat tacctggcgt tgagtttttg aaatggtgta ataaccacaa
     5641 ctcaaagatg tggaaaatgc gcgtcattca tttcgccatt aattatcact gtgctcatta
     5701 attaacagaa cacgtataat gagagccatc tcgcaaaaat gaaaaaacgt tttataaaat
     5761 catcacttca tcatgaattc aaattcatcg attaatatca acaagataca aaaagcacta
     5821 tcattaaaat tcattgcatt tacattgatt tcaccaatga aatgtaaaaa tatataaact
     5881 tgatgattta agcattttct tataaccgtt cagacgttat tcttatttca gatcatcgtc
     5941 agaattgact ccacgatcac atttcggacc ggcagaaagg aattattctg caaacagtaa
     6001 ttatggtgtt ttgatttatc ttgcacctct ccacttctgg atataaggat attaggtatg
     6061 gcaaccgctg gaatgcttct caaactcaac tctcaaatga accgcgagtt ttacgcatcc
     6121 aatctctacc ttcacctgag taactggtgt tctgaacaga gtctgaacgg caccgccact
     6181 ttccttcgcg cccaggcaca gagtaatgtg acccaaatga tgcgcatgtt taactttatg
     6241 aagagtgtcg gcgctacccc catcgttaaa gccattgatg ttccaggtga aaaactgaac
     6301 tctctggaag aactgttcca aaaaacgatg gaagaatacg agcaacgttc aagcactctg
     6361 gcgcagttag ccgatgaagc gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc
     6421 gatctggaaa aagaacagca gcatgatggc ctgttgctgc aaaccattct tgatgaagtg
     6481 cgcagtgcga aacttgcggg tatgtgccct gtgcagaccg accaacatgt tctgaatgtc
     6541 gtgtcacacc agctgcattg atcatacatc ggcgctaata cattgcgccg atgaagtttt
     6601 tgagaaaccg ctgcctcatc tgtttgaagc agcggttttt ttaatgggat tcaccctgtg
     6661 gggtaaactt gagttcaatc agcgcgatgg ctttttggat tgcccgcatg gtgaccgggt
     6721 ctgcggcagc gggatgatga gtaaagtcga tattcttcag ctgactggac attttttcac
     6781 gaacttcaac gggcgcgatt acatcgagaa catccagaat ttgtttgata accaactggc
     6841 aagctaccac atcagaaacc agttcctgat cggcattcag cggctgggac atcgtaaact
     6901 cctgatagca ttttgaaagc cgttatagta gcggcttcac atcttcagcg atagtcacat
     6961 ccaccgtcat caggacacaa aaaaacctgc cggagcaggt tttttgttat cggaacatat
     7021 tgcctggcgg tacgtctttg aacgtcttgc aatagttatt gaacatactt ttcaggattt
     7081 tgcgcagttt catcgcggca ctccgaccat ttgttataca ggtgttattg tctttgcgct
     7141 tataatatga caaccatcac aaaaatcaat ctttatgtga tacaaatcac ataaataccc
     7201 ctttaatgtt ataaaaatga taatcaaaaa acagcccccc tatttctgac acctacagat
     7261 ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt gtcgcagcgc tgtgacgcgt
     7321 aaaatagtgc tttctcttac tcttctggct ggaccatgag acttctgatt ctgactcttt
     7381 cattaataac gctggcgggg tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg
     7441 cggcaacagg tattgtacgg ttggtttatg accaggcttt tttgcagcat gcccataccg
     7501 atcgttatgt cagtcgcggg atcgccgatc gcgcatgcca gcaggaaggc tatacccacg
     7561 cgatcccctt tggtcagcca gtaggcaact gtagcctttt tgcgggttcc ttatgtttga
     7621 atacggaatt cactttgtca tatcagtgcc atcattccgc cttccctgtc tttctctaaa
     7681 tattttaatc agcgagggga tcttcgctga ttaaagaaat agcggtaatg cgttttattc
     7741 ccattcgtat ttttaataat tgaagtttat attttacctt ttgcaaataa taaaataaca
     7801 aattatagtg acgccacagc aacaaatata acctttgtgg agcactatca tgctgaaacc
     7861 agaaatgatt gaaaaactta atgagcagat gaacctggaa ctgtactctt cactgcttta
     7921 tcagcaaatg agcgcctggt gcagctatca taccttcgaa ggtgctgccg cgttcctgcg
     7981 ccgtcacgcc caggaagaga tgacgcatat gcagcgtctg tttgattacc tgactgatac
     8041 cggcaattta ccgcgtatta ataccgttga atctccgttt gctgaatatt cctcacttga
     8101 tgaattattc caggaaacct ataaacacga acaattaatc acccagaaaa ttaacgaact
     8161 ggctcatgct gcaatgacca atcaggacta cccaacattt aatttcctgc agtggtatgt
     8221 ttctgagcag catgaagaag agaaactgtt caaatcgatt attgataaat taagcctggc
     8281 aggcaaaagc ggcgaaggtc tgtattttat cgacaaagaa ctctctaccc tcgacacaca
     8341 aaactaatgc tctccggcgg caggtttctg ccgccgtttc aaatcagtga cggcaaatct
     8401 tactctgatc ggtcgaaaaa ccttcttctg aaggtataaa tttgcctttt gctgccagaa
     8461 acgctaccag ctctcccgct gtcacccctt ctgccgagca cgtgtgaaaa cgtgcctgtt
     8521 caccaaaacg cgctttaatc gcagcttcca gactggcatg cgtatattgc tcgcctgatt
     8581 caatcatcat atttaacact tcatgaccgt gaatagagtc catcgttcct cctcaaaaaa
     8641 agcctagcgt agcgattacc gcttatgaag actttgcgcc agcgcaggac tgaatgcttt
     8701 tattgtacat ttatatttac acaatatgta acgtcggttt gacgaagcag ccgttatgcc
     8761 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     8821 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     8881 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     8941 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     9001 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     9061 gggcttcagt atgatgttct taatgtatgc tctgactgca gcatacatca gcggtgccgg
     9121 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     9181 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     9241 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     9301 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     9361 actgtctgca atcccggtga tttttacgtc gtttggtttt cacggtagcg tgccgagtat
     9421 tgtcagctat atggatggca acattcgtaa gctacgctgg gtgtttataa tcggtagtgc
     9481 catccccctg gtggcatata ttttctggca ggtggcgacg cttggcagca ttgattcaac
     9541 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     9601 cgaaatggtg gcctctccgc atgttgagct ggcggtgcat ttatttgctg atttagccct
     9661 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttgtttca
     9721 gcgttcaaat accgttggtg gacgattgca aactggtgca attacctttc tgccgccgtt
     9781 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     9841 gctggcggta ctggcattga ttatcccttc actattgacc tggcaaagca gaaagcacaa
     9901 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     9961 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
    10021 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatgtg
    10081 acctcttaat gcaggcagca ctgcttaaat ttcttaccac tgccgcacgg acaaggatcg
    10141 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
    10201 gccatccagt atgcatgtaa atcaagcgcc gccagtcgaa tggcatctac gctctcttca
    10261 aacgcttctg gcgacatttt ctctacccgc tcgaagtttt cctcagtacc gtgcagcgca
    10321 atcgcctcca gcgctggttt taaagaatcg ggcaacgttg accagtcaga aagtgccacg
    10381 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
    10441 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
    10501 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccaacgc
    10561 ggcacatagt cagccccacc ccacacggca accagccact gttctggttc aatctcttgc
    10621 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
    10681 tcagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
    10741 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
    10801 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
    10861 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttatta
    10921 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
    10981 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
    11041 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
    11101 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
    11161 atgtctttta aactggagcg ggaaacgaga ctcgaactcg agaccccgac cttggcaagg
    11221 tcgtgctcta ccaactgagc tatccccgca ttcatcaagc aatcagttaa tcacttgatt
    11281 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
    11341 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
    11401 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
    11461 cagtaccgca gccacaaaga aaagtgcaat accgacgtac tcaacccaaa tgttcggacg
    11521 ccacagcagc caggccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
    11581 gacggccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
    11641 aatttcacgg gcgatcatcg ttgccgccgg taatgtcacc caccagctgt gataatgctc
    11701 ggttaccagc accatggcga tagccacgag aactttatct gccacagggt caaggaaagc
    11761 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
    11821 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
    11881 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
    11941 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
    12001 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
    12061 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
    12121 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
    12181 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
    12241 atatgctgga taacatgcag cgcgggtgaa tctggcggca aactaaatcc ctcaccttcc
    12301 ggctcaaaga acagcgtttc cagtccagcc ttacgatctg ctcctttggc aacgccaagt
    12361 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
    12421 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
    12481 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
    12541 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
    12601 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
    12661 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
    12721 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
    12781 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
    12841 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
    12901 aaggtgcgca tctggctgcc ttgtaaatag aactgaccta cgaaggtttc taccacctcg
    12961 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
    13021 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
    13081 tcaaggtcgt cgccggtatt ggaaacaaac tgtttttcgg tgacgcgtcg caccgcctga
    13141 atttggtcgc gaatacatgc cgcttcttca aactccagat tctggctggc agtttccata
    13201 cggctaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
    13261 tcgacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
    13321 cgccctatct ggtattgcag acacggacgc gagcgattgc gataaacact attttcgcac
    13381 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
    13441 gggaacgggc cgaaatattc acctttggca tgctttgcac cacgatgcat cgccagacgc
    13501 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
    13561 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
    13621 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
    13681 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
    13741 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
    13801 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
    13861 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
    13921 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
    13981 agatctcatt gaccttctgg cccttagtga tcatcagcat aatctgcaat tcacgttcag
    14041 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
    14101 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
    14161 cttcctgcgg agccgcgcct ttgctgaggt agccagcagc acctgcctgc atgactttcg
    14221 ctggtaaagg gttttctgta tggaccgtaa gcatgatgat tttaacatca gctgtggaac
    14281 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
    14341 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctctc
    14401 cgacgacttt tataccctta atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
    14461 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
    14521 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
    14581 tggtgggtat tcgatttttc tctcgttttt gtcaattcga ttgtccacag ttatggtcac
    14641 gcccgtcacc aacggcttac cggatcgttt cacagtaagc cctacgatga aatgtgacaa
    14701 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
    14761 ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg
    14821 tattttatag cactgccgat atcacgctca aaaaacaacc actgctattt ttagcaaagc
    14881 ttatggtata ctccgccgcc ttaacatttt tcaccacaaa ttttcattgc aacatgacga
    14941 ggaaaataaa tgagtacgcc tggtttttct actgccgaga ataatcaaga actggcaaat
    15001 gaagtctcct gcctgaaagc gatgctgacg ctgatgctgc aggcgatggg acaagctgac
    15061 gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag
    15121 gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa
    15181 accggctgag agcgtgcctt tcagccggtt attgacaaaa tcatcgctat ctctgctgat
    15241 gtcttccctt ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa attcatctct
    15301 ggcgtcatca ctatttcatc atttaaacgc atcagagcca tcagactttc gcgcaccagt
    15361 aactgcattt ttaattgcag ttcatcacta agaatgggta tttcgcgcgt gctgcaacgg
    15421 gaaaaggaca aaaagcccag cgcccgattt ggcagcatta aatactgagt gacaccgcgg
    15481 cgtaaaccat gtgcgtgcgc ggcttcccat aatggctgtg cttcgctgaa taagtcatca
    15541 ttccacatta aatggccctg actaaagttt tcagggttca gcacaggatc aattgcgaga
    15601 aaattttttg cctgataata actaacccac gactcagggt aattggtgta gaaagccact
    15661 ttaggtcgcg tgaatggtac cgggtggcga acacataacg aatagtaatc gtactccagc
    15721 tgctgagcct gaagctctat ttcatggtag gcctcttctg cggcctccat cctctgaaaa
    15781 cgcaacagca tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt aaaccgcaac
    15841 gcccctgaga gtgagtatca tttataatga taattcaaac tatcacataa ataagattta
    15901 tatataattt atattattca ggcaatgaat tacttttgca agccatcgca ttctcttatg
    15961 ttattaatga gttatgctga tttgttaagc agttttatca cgcttgaaat ggcgtcccgc
    16021 cccgacaggt gaatcgtcgg ggctgatttt ttcttattat tgcagcagaa gcttctcgag
    16081 gaactggcgg gtgcgaggct gctggggatc ggcaaataac gcttttgctg ccccctgctc
    16141 gactatccgc ccctggtcca taaagatcgc ccggtccgca acatcccggg caaagctcat
    16201 ttcgtgtgtc acaatcacca tcgtgcgctt ttcctgcgcc agctggcgga tggtgttcag
    16261 gacttcgccc accagctctg gatccagcgc cgacgttggc tcgtcaaaca aaatcacctc
    16321 aggacgcatt gccagtgcac gcgcaatcgc aacacgctgc tgttgaccgc cagacaaacg
    16381 acgtggatag ctggtttctt taccggccag cccaactttc gccagcagct cgcgagcgcg
    16441 cgccgtggcc tcttctttcg gttcgccttt gacgatcacc ggcccttcaa taatgttttc
    16501 cagcaccgta cgatgcggaa acaaattaaa gttctggaag acaaacccga cgtgctgacg
    16561 caactggcga atcagcgatt tttgctgact taatgaacgt gcagtatcaa tagtgatatc
    16621 gccaacggtg atcgttcccg cttccggttg ttccagcaga tttatgctac gtagcaatgt
    16681 ggttttgccg gaaccactcg gaccgataat tgccaccact tcgccaggct ttacctcgag
    16741 gtcgataccg tgcagcaccg tctgaccgtg gaattttttc accaggttct taacttcaat
    16801 ggcactcatt ttggttctct ctcctggcga ttaagttgat tctcaaaatg gttctgcaac
    16861 gtcgataaca cagtcgccat gatccagtag atgagcgaag ccgccaggta catggtgaac
    16921 acctccagcg tgcgcgaggt aatcaactgc gcctgacgga acagctccgg cacctggatt
    16981 gtcgcggcca gcgacgtatc ttttaccaga ctgataaagc tgttcgacag cggcggcaac
    17041 gccacccgcg ccgcctgcgg caaaatcgcg cgacgcatgg tctgccacgg cgtcatacca
    17101 atactggcag cggcttccca ctgaccttta tcaatagaag aaatggcggc acgcagcgtt
    17161 tcggcggcat aggcggcagt gttcagcgac agaccaatca ttgctgacgg aatgggatct
    17221 aattcgatac caaactgcgg caggccgtaa tagatcataa acagttgtgc gatgagcggc
    17281 gtaccacgaa aaatagagat ataaaaacgc gccagccagc gcacgggcca gattggcgac
    17341 aggcgcatca gcgcgagaat aaacccgagt agtaagccaa aaaacatgcc gccgatactg
    17401 agttgcaggg tatacccagc ccctttcaac aggaacggca aggaatcaat aaccagttgt
    17461 atactttctt gcatgagcgt tcccggagct aaacgtgggg atgataggcg aacagcgcag
    17521 gcgcgccgcc ggtatgaata aacagaatcg gcccttcatc tttgaagcgt ttctgactga
    17581 taccgtcaat cagccccgcc atcgcttttc cggtatacac aggatcaagc agaatgcctt
    17641 ccagacgcgc cagcaatttc actgcttcca tgccttcgtc gttcggcacg ccgtagccag
    17701 gtgcaaaata gtcatcccag agtaaaattt ccgctgatgc ggtcagctcc agttctttcg
    17761 caatcgcctg ttgtaggtta accactttcg gcaattgatc ggcaacggaa cgcgacacgg
    17821 tcacgccaat cagttcgctt tcaggcatca ggtgttccag cccaacagcc agtccggcgt
    17881 gagttccggc actgcccgat gcgactacca ccgacgaaat attaaccgcc ccttcacact
    17941 gttgcgcgat ttccagcgca ctctccacat aacctagcgc gccaagagca ttagaaccgc
    18001 caaccggaat gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc agttcttcca
    18061 gttgggcatt gggatcggtc agtgcgtcgc acatttcaat ctgggtattg aacagatcca
    18121 gcaacaaacg attgccgttg gttaaatagt tttctgcggt tgtgccaata ggattttcca
    18181 gcagcgccac gcagtgcaga ccgagtttcg ccgcgactgc ggcagtctgg cgcacatggt
    18241 tagactggat cgcgccggca gtaatcagcg tatcggcacc ttcgcgcagg gcatctgccg
    18301 cgagaaattc cagcttacgt aatttattgc cgcccattgc catgggggtg acctcatccc
    18361 gtttgatgaa aatttcccgt ccaagataat cagaaaagcg cggcagatat tcgagcggcg
    18421 ttggcgcgcc gataaactcc aaccgtggaa aacgggttaa attatgcagt ggcataacag
    18481 cctccgatgt gtgttgttgt gattttctta ttatgcacgc tgaaaacgcg taaataaaaa
    18541 aggcgctagt gaaagcgccc ttttttgtca ttatgctgat tatttggtca catcagcacc
    18601 aaaccatttt tcggaaagag cttgcagagt gccgtctttt tgcatttccg caattgcatc
    18661 attcaccgct ttcagcaggt cttcatttcc tttacgcagc gccacgccag actcctgacg
    18721 ggagaatgct tcaccggtta ctgccagcgt atcgttggtt ttcttcacca gatccagcgc
    18781 cgccagacga tcaacgagga tcgcatcgat acgccctacg cgcagatcct gatatttggt
    18841 cgggtcatca tcataggtac gcacatcgac accctgaaca ttctgccgca gccactcttc
    18901 atagttggtg cccagaccga cacccacttt tttgcctttc agatcagcag ccgttttaat
    18961 ggtgccttcg ttaccttttt tcaccagcgc ctgaataccg gaaatggtgt aaggggtaga
    19021 gaaatcgtat tttttcttgc gctcgtcaga aatggtgacc tgattaatca ccacatcaat
    19081 gcgtttagag tccagcgacg ccagcatacc gtcccatttg gtcggtttta gtgacgcctc
    19141 aacgccaaga tgttttgcca gctgttgggc aaattccact tcaaaaccgg ttaatttgcc
    19201 gtcatccccc tgaaaactga acggcggata agtgccttcc agccctacca gcagcgtgcc
    19261 gctctctttg actttattaa gcagaccttc atctgcaaaa cttttaacgc tcatgcccgc
    19321 aaccagcgcc acggccatca cacccatcaa tgcctgacgt cccagatgtg ctaatttcat
    19381 attcaccccg aatgttgtta tgtctgtttg cagtgtagag ccattgtttg taaacacaaa
    19441 aacaactccg ctacatctta ttcttattta atatatatca gaagcaggca ggctggagga
    19501 tttctgcaca agtccggtac aggtttggcg ctgatagtgt tgatacttcc gtaatgcgat
    19561 gcggtaattg ttggtgctgg tggtgggcag ccacggggcg aggatttcat caaggaaacc
    19621 gtcctgcagc tgatcgaggg aaatattttg ctcatgtaga tgattaccca aacggcgcag
    19681 acgaacgaca tattcacgca ccgtacctgg gctcatttca gtttgctcaa aaagaaattt
    19741 cttaaagccg ataatatcga aaaaatcact ctgcgttttg caatgtaaat caccgcaaaa
    19801 tcggcacaat gccgcccatg attgttgttc cgtttgccag cctttttcat caagcagcgt
    19861 gtccaggcgg gaaatctcga ttttattcac tattttgccg tcgcgaacta aggtaatgcg
    19921 atcgagtaat ttacggcaat gcgcgcaatg ggtctggctg tgtttaaagt ctttaagata
    19981 gcggcttaat ggccgtcttt tcaggtgctg caccatcatt aagaactcct ggtagtcaaa
    20041 gttaaagtgc ggcatttact gacgttataa cttacccagt ttagtgcgta accgtttaat
    20101 ggcctggctg tgtaactgac tgacccgcga ttccccgacc tccagcaccg cgccaatctc
    20161 tttgagattc agctcttcct ggtaatagag ggttaatacc agtttttcgc gctccggcaa
    20221 cgtttcgatg gcttccatca cccgctggcg cagattactg tccagcagtt gttgtagcgg
    20281 gttttctcgc tgatgatcat cagtaaccag ttcgatgcta tcgccgtgct cttcacgcca
    20341 ctcatcgtag gagaagagct ggctgttatt ggtgtcgagc agcatttgac gataatcggc
    20401 aatatcgatc cctaaacgtt ccgctacctc agtttccgtg gcgttgcggc caagttcctg
    20461 ctccagttgc cctattgcct gtgccacttc acgcgcgttg cgtcgcacgc tgcgcggcac
    20521 ccagtcacgg ctgcgaagtt catccagcat agcgccacgg atacgctgca ctgcgtaagt
    20581 tgtaaatgcc gttccttgta gggcgtcata gcgttcgaca gcattaagta acccaatgcc
    20641 gcccgcctgt agcagatcgt caagttccac gctcgcgggc agtcgaacct gcaggcgcaa
    20701 tgcttcgtga cgcaccagcg ggacataacg ctgccacagc gagtgtttat ccattacacc
    20761 ttcagcggta tagagtgaat tcacgataaa cagccctgcg ttatatgagt tatcggcatg
    20821 attatccgtt tctgcagggt ttttaatcgg acgattagtg ggtgaaatga ggggttattt
    20881 gggggttacg caaaaagccg aatgtaaaaa accccgccgg tggcggggtt tgagaggcat
    20941 tcagacgatt aaccctgcag cagagacaga acctgctgcg gtacctggtt ggcttttgcc
    21001 agcacggagt taccggcctg ctggataatc tgcgctttcg acatgttgga cacttcggtc
    21061 gcatagtcgg cgtcctgaat acgggactgc gcttcagaca ggttggtagt ggtgttgttc
    21121 aggttggtga ctgcggaatc caaacggttt tgcaccgcac ccagggagga gcggaatttg
    21181 tcgatggagc tgatggcttc atccagagct ttcaggggat cggctgtcgt agctgctttt
    21241 gtttcagcat ctgtcgtcaa tttaccatca gcatctttat aaactgcctt accgctgccg
    21301 ttagtcactg aaccatcatc ctgtaggtaa aaatctacag tatcagatgt ggtcaacgcc
    21361 tcgttgttat caacataaac cgaatcaaca ccttttgtta cagcgccagt tgtgccgttg
    21421 acgctataac ctgcagtagt gaaatcaaca ttgtttcccg aggttgatac agctttatcg
    21481 aaagctgcct gatcaacttt tgcacctgtt actgaataag taatagtgtc cggcgtagta
    21541 ctattaccgg taaaggagat agaggtaccg ccaatgtcaa tggttgtcgc tttggtatta
    21601 ttggctgcaa gtgtggacag actagcctca gtcaaagaag caccagagcc gtttttcgtt
    21661 aagcgcccag ttgtatcaat gtagagttta tctccattgc ttgccgtaat tttaccatca
    21721 ctgctgatct gaacatcctg tgcagaacca tcgatagtaa tagtgccctt agcggtatca
    21781 ccaactcccg gggtcaaata tttctgaaca tcggcagctg aagccgttgt ggtatcaaaa
    21841 gagtatgact tacttgcact gtcatacttg taattcgttg cactagccgc cgttccgaag
    21901 ccgttattaa tggttgcctg aacagtatca ccatcactaa ctccagccag caaatcagac
    21961 gctttagcag catcgtaacc cgcactcaca gtatatttgt tgccgaccac tgttgcatta
    22021 gcagctacca agtcagcttt agatgcagca gtattagcca cagccccgcc accattcaca
    22081 ttaaacccat tcagccccag cgtatcagag tcaattttct tcagatcaat agtgatagtc
    22141 tggccgtcat tcgcaccaac ctgaattttc atcgaaccgt cttttgccag tacgttcacg
    22201 ccgttgaact gggtctgacc ggatacgcgg tcaatttcgt cgagacggga tttgatttcg
    22261 tcctgaatgg agtccagatc cgaatcagag ttagtcccgg tagaagcctg aaccgtcagc
    22321 tcacggatac gctgtaagtt gttgttgatt tcggacagcg cgccttcagt ggtctgtgca
    22381 acagaaatac cgtcgttggc gttacgtgca gcctgagtca ggcctttaat gttagaagta
    22441 aaacggttag caatcgcctg acccgcggcg tcatccttcg cgctgttaat acgcaagcca
    22501 gaagacagac gctcgataga actcgacagc gcagactggt tcttgttgat attattttga
    22561 gtgatcagcg agaggctgtt ggtattaatg acttgtgcca tgattcgtta tcctatattg
    22621 caagtcgttg attacgtttt gggtttccac ccgtcggctc aatcgccgtc aaccctgtta
    22681 tcgtctgtcg taaaacaacc tttagaattt ttttcacaaa cagccatttt ttgttagtcg
    22741 acgaaatact cttttctctg ccccttattc ccgctattaa aaaaaacaat taaacgtaaa
    22801 ctttgcgcaa ttcaggccga taaccccggt attcgtttta cgtgtcgaaa gataaaagga
    22861 aatcgcatgg caagtatttc atcgctggga gtcgggtcag gtctggattt aagttcaatc
    22921 cttgatagcc tcaccgccgc gcaaaaagcg acgctaaccc ctatttcaaa tcagcaatcg
    22981 tcgtttaccg ctaaacttag cgcctacggt acgctgaaaa gcgcgctgac gactttccag
    23041 accgccaata ctgcattgtc taaagccgat cttttttccg ccaccagcac caccagcagc
    23101 accaccgcgt tcagtgccac caccgcgggt aacgccatcg ccgggaaata caccatcagc
    23161 gtcacccatc tggcgcaggc gcaaaccctg accacgcgca ccaccagaga tgatacgaaa
    23221 acggcgatcg ccaccagcga cagcaaactc accattcaac aaggcgacga caaagatccg
    23281 attaccattg atatcagcgc ggctaactca tcgttaagcg ggatccgtga tgccatcaac
    23341 aacgcaaaag cgggcgtaag tgcgagcatc attaacgtgg gtaacggtga atatcgtctg
    23401 tcagtcacat caaatgacac cggccttgat aatgcgatga cactctcggt cagcggtgat
    23461 gatgcgctac aaagttttat gggctatgac gccagtgcca gcagcaacgg tatggaggtc
    23521 tcggtcgccg cccagaatgc gcagctgacg gtcaacaacg tcgccatcga gaacagcagc
    23581 aacactatca gcgacgcgct ggaaaacatc accctgaacc tgaacgatgt caccacgggc
    23641 aaccagacgc taaccatcac tcaggacacc tccaaagcgc aaacggcgat taaagactgg
    23701 gtgaatgcct ataactcgct aatagatacc ttcagcagcc tgaccaaata caccgccgta
    23761 gatgcgggag ctgatagcca gagttctagc aatggcgcac tgctcggcga ctccacgctg
    23821 cggacgattc agacgcagtt gaagtcgatg ctgagtaata ccgtcagttc ttccagctat
    23881 aaaacgctgg cgcagattgg tatcacgacc gatcccagcg atggcaaact ggaactggat
    23941 gccgacaaac tcaccgctgc actgaaaaaa gatgccagcg gcgtaggtgc attgattgtt
    24001 ggcgatggta aaaaaaccgg catcacaacc accatcggca gcaacctgac cagttggctt
    24061 tcgacaacgg gcattattaa agccgctacc gatggcgtta gtaaaaccct gaataagtta
    24121 actaaagact acaacgccgc cagcgatcgc attgatgcgc aggtcgcgcg ctacaaagaa
    24181 caatttaccc aactggacgt tttaatgacc tcgttaaaca gcaccagcag ctacttaacg
    24241 cagcagttcg aaaacaacag taattccaag taatcattat tcatcgggag acaggtcatg
    24301 tacgcggcaa aaggcaccca ggcctatgca caaattggcg tcgaaagcac cgtaatgagc
    24361 gccagccaac agcagttggt caccatgcta tttgatggag tgctgagcgc actggttaga
    24421 gcgcgactgt ttatgcagga caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg
    24481 atcaacatca ttgagaacgg actgcgggtg agtcttgatg aagagagcaa agacgaatta
    24541 acccaaaact taattgctct ttatagctat atggtcaggc gtttgctgca agccaattta
    24601 cgcaacgatg tctccgcagt cgaagaggtg gaagcattaa tgcgtaatat tgccgatgcc
    24661 tggaaagagt ctttactatc cccttcttcg attcaggacc cagtctgatg aacaatgcac
    24721 cgcatttgta tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg ttacgcctgg
    24781 caacggaaga acaatgggac gaactcatcg ctagcgaaat ggcgtatgtg aatgcggtgc
    24841 aggagatcgc acacctgact gaagaggttg ccccgtccac tacgatgcag gagcagctcc
    24901 gcccgatgct gcacctgatt ctcgacaacg aaagcaaggt aaagcagtta ttacagattc
    24961 ggatggatga actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg gtgttaagtg
    25021 cctatggcga tcagggcggc tttgtgctgg ctccgcagga taacctcttt tgaatcttaa
    25081 tgagccgatg gctcgcgaat aatccgatta cggctacgct tctaatattc cccttgaatg
    25141 gagtcgaaga atgcgtaatc ccacgctgtt acaatgtttt cactggtatt acccggaagg
    25201 cggtaagctc tggcctgaac tggccgagcg cgcagacggt tttaatgata ttggtatcaa
    25261 tatggtctgg ttgccgcccg cctataaagg cgcatcgggc ggctattcgg tcggctacga
    25321 ctcctatgat ttatttgatc taggtgagtt tgatcagaaa ggcagcatcc ctactaaata
    25381 tggtgataaa gcacaactgc tggccgccat tgacgccctg aaacgtaatg acattgcggt
    25441 gctgctggac gtggtagtca accacaaaat gggcgcggat gaaaaagaag ctattcgcgt
    25501 gcagcgtgta aatgctgatg accgtacgca aattgacgaa gaaatcattg agtgtgaagg
    25561 ctggacgcgt tacaccttcc ccgctcgtgc cgggcaatac tcgcagttta tctgggattt
    25621 caaatgtttt agcggtatcg accatatcga acaccctgat gaagatggca tttttaaaat
    25681 tgttaacgac tataccggag aaggctggaa cgatcaggtt gatgatgaac tcggtaattt
    25741 cgattatctg atgggtgaga atatcgattt tcgcaatcat gccgtgacgg aagagattaa
    25801 atactgggcg cgctgggtga tggaacaaac gcaatgcgac ggctttcgtc ttgatgcggt
    25861 caaacatatt ccggcatggt tttataaaga gtggatcgaa cacgtacagg aagttgcgcc
    25921 aaagccgctg tttattgtgg ctgaatactg gtcgcatgaa gtcgataaac tgcaaacgta
    25981 tattgatcag gtggaaggca aaaccatgct gtttgatgcg ccgctgcaga tgaaattcca
    26041 tgaagcatcg cgcatgggtc gcgactacga catgacgcag atcttcactg ggacgctggt
    26101 ggaagccgat ccttttcacg ccgtaaccct cgttgccaac cacgacaccc aaccgttgca
    26161 agccctcgaa gcaccggtcg aaccgtggtt taaaccgctg gcgtatgcct taattttgtt
    26221 gcgggaaaat ggcgttcctt cggtgttcta tccggacctc tacggtgcac attacgaaga
    26281 tgtcggtggt gacgggcaaa cctatccgat agatatgcca ataatcgaac agcttgatga
    26341 gttaattctc gcccgtcagc gtttcgccca cggtgtacag acattatttt tcgaccatcc
    26401 gaactgcatt gcctttagcc gcagtggcac cgacgaatat cccggctgcg tggtggtcat
    26461 gtcgaacgga gatgatggcg aaaaaaccat tcatctggga gagaattacg gcaataaaac
    26521 ctggcgtgat tttctgggga accggcaaga gagcgtagtg acagacgaaa acggcgaagc
    26581 gaccttcttt tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg tgatttaaat
    26641 ccatccccgg cggcaagccg gggagatttc attacggcag tggcgtcggt aacggcgctt
    26701 tatccagcgc ggcggcacac tctgccgtcg ggcgatccac acgctccatc gtcataccat
    26761 cgtactcaat ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt acggtaacgt
    26821 tagtgagatc atcagacatc aatgtcaact tacccggcac ggcaatcccc cgctgccact
    26881 gacggcaatc caaggtatcg ccctctttag tgacaatcag gctgcctatc gcttccggac
    26941 tgaccagcgc acgttgcggc cctttggtct gccagtagcc cgccagccag tccggcgcgg
    27001 gggttttcac gacattgtta tagttttcga cttcggcaca gcccgccagc aacatgagcg
    27061 cgcctgcaat tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag aggattgtgg
    27121 cattaaagct ctgatgtcgc cagcctttcc aggttcagag agaaatttga tctaactatt
    27181 atttttgtga attattaccc cgaaaataaa tttgcatggt gtatgattcg cggctcttgt
    27241 ttcaaacctt ctgagttcag aggctacatt catgtcatgg cagcaattca aacacgcctg
    27301 gttgattaaa ttctgggcac ccatccctgc ggtcatcgcg gcgggtattc tctccactta
    27361 ctattttggc attactggca ccttttgggc tgtcacgggt gaatttaccc gttggggcgg
    27421 tcagctcctg cagctgtttg gcgtccatgc tgaagagtgg ggttatttta aaattatcca
    27481 tctggaagga tcgccattaa cccgcatcga cggcatgatg atcctcggta tgtttggcgg
    27541 ctgctttgct gctgcgctgt gggccaacaa tgtcaaacta cgcatgccac gcagccgtat
    27601 ccgcattatg caggccatca ttggcggcat tatcgccggt tttggcgcgc gtctggcaat
    27661 gggctgtaac ctggcggcgt tctttaccgg cattcctcag ttctcgctgc atgcctggtt
    27721 ctttgccatc gccactgcca ttggttcatg gtttggcgcg cgctttaccc tgctgcccat
    27781 cttccgtatt cccgtgaaaa tgcagaaagt ttctgccgcc tcaccgctga cgcaaaaacc
    27841 ggatcaggcg cggcgtcgtt ttcgtctcgg gatgctggtc tttttcggca tgctgggctg
    27901 ggcgctgctc acggcgatga accaaccaaa actggggctg gcaatgctgt ttggcgtcgg
    27961 ctttggttta ctgattgaac gtgcgcaaat ctgctttact tcggcgttcc gcgatatgtg
    28021 gatcaccgga cgtacccata tggcgaaagc aatcattatc ggtatggcgg tgagtgccat
    28081 cgggatcttc agttacgtac agttaggcgt tgaacctaaa atcatgtggg cgggaccaaa
    28141 tgcggtaatt ggtggtttac tttttggttt tggcatcgtg ctggcaggcg gctgcgaaac
    28201 cggctggatg taccgcgcgg tagaaggcca ggtgcactac tggtgggtcg gtctgggcaa
    28261 cgtgatcggc tcaacgattc tggcgtatta ctgggatgat ttcgctccgg cgctggccac
    28321 cgactgggac aaaatcaacc tgctgaaaac ttttggtccg atgggaggcc tgctggtgac
    28381 atatttgctg ctgtttgctg cgctgatgtt gattatcggc tgggaaaaac gcttcttccg
    28441 ccgtgaggca ccgcagactg ctaaggagat cgcatgaaaa atatcgttcc tgattaccgc
    28501 cttgatatgg tgggtgaacc ctgcccttat ccggcagtcg caacccttga ggcgatgccg
    28561 caattaaaaa aaggtgagat cctggaagtg gtgagcgact gtccgcagtc gatcaataat
    28621 attccactgg atgcgcgtaa tcacggctac acggtgctgg atattcagca agacgggccg
    28681 accattcgtt atttaattca gaagtaatcc ctcattcccg ctgtggccca cagcgggaac
    28741 atcgttacct ctgacacata gattttaccc gccccctctc ctattctttt cagtatgatc
    28801 tcaggaggtt gctatgtgtg gacgctttgc ccaatcccaa acgcgtgagg attaccttgc
    28861 gcttctcgcg gaagatattg aacgcgatat tccctacgat cccgaaccca ttggcagata
    28921 caatgtcgcg ccgggaacca aagttcttct gctcagtgaa cgtgatgaac accttcatct
    28981 ggatccggtt ttctggggat atgctcccgg atggtgggat aaaccgccgc tgattaacgc
    29041 ccgcgtagaa actgcctcaa ccagtcgcat gtttaaaccg ctctggcaac atggtcgggc
    29101 aatctgtttt gccgatggct ggtttgagtg gaaaaaagaa ggcgacaaaa aaagccttat
    29161 tttatctatc gcgctgacgg acaacctgtt tttatggccg cgataggtag cataccgttt
    29221 gagcgtggtg acgaagccga aggatttttg atcgtcactg cggcggcaga tcaaggtctg
    29281 gtagatattc atgaccgccg cccgctggtt ctgtcgccag aagctgcacg ggaatggatg
    29341 cggcaagaca ttggtggtaa agaagcctca gaaatcgcta ccagaagctg tgtgccagca
    29401 aaccagttca cctggcaccc tgtgtcgcgc acggtgggta atgtcaaaaa ccagggcgcg
    29461 gagttaattc aacctgtttg ttgacatcct ccacgccctg aatgacgagg acccctgcta
    29521 cgttcaggct gtcgcctgaa tcatttcggt gggttcctgc ttcaacgagc ggcctgactg
    29581 caccatctct ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac gagctccatt
    29641 tacatcggcg ttcgctgtat atccacatac ctggcatctg aatttacttt gtgacaggcg
    29701 attttctttc gctgtatgac cacagcacgc gcaacgctgg cttgtgtacg ctggcggaac
    29761 agcaagcacc tgaccgccac gccagagctg cttgtactca agctggcggc gcatttcata
    29821 ccagccctga tccagtatcg aacggtttaa acctgatttt gcccggacat tgcgccccgg
    29881 ctggctgacc gtacccgctg ctgactttga catgttgctg accttcaaat cctcaatgac
    29941 tatcattacg tggtttttgc tgacggtcgt tgtgactttg tgaaggtagt ccctgcggat
    30001 atttgcgata caggaatgca gtcgctgtat tttgcgtttc tgcttctgcc agttgttgct
    30061 gaatttgacc ttgcggctta actgtcgctg aagtctcgcc agcttcttct ggtttttctg
    30121 aaaactgttt acaggctcaa agactgtgcc atctgacagc gaggcaagtt tagccacgcc
    30181 agcatccagc ccgaccattg atgctgaagg gtgaaccgga gttgatactt cactttctgc
    30241 ctgaatactg atgtaccact taccgcagga ctggctgaca gtgacatttt tcacaacacc
    30301 cgtgacctga cggctgttcc ggtagcgcat ccagccaagt tttggcagaa aaatacggct
    30361 gttttcctga gcgagcttaa cgccctgcgg atagcggaat gcatcattct gtcaccgctt
    30421 tttgaatcag gggaacgccg agcgcttctg gaaaaagttt ttgcaggcct gctcagggtc
    30481 tttcagtgac tgttgcaatg gctgtgacgg agcatcttta atccattgcg tttcagtggc
    30541 atttttccac tcaaccaatc aggaagccat tttcgcgtaa gggatgtctt tgttaccggc
    30601 cttatgattc tcattctgac gtgccagagt acgactgaaa acgaaacgac atgcgcctgc
    30661 gaagcgcctc atttcacgcg cctgttgacc accgggtctt aaccggaatt tgaatacctg
    30721 tagtcgtttc acagggcaaa ttatactttg gtctgtgaaa aaaactgata tccgacgcgg
    30781 aaggcactgt gttttcctga tgcatgctca ccgggtcttt gttaccaagt accgacgcca
    30841 gatttttgac catgacgcga cagaaaaact acgcacttac ttttcaaatg tatgtgctta
    30901 ttttgaagct gaactggttg aaatggatgg cgaaccagat cacgtccatc tgttaattaa
    30961 ctatcccccc aaactggcga tatccagtct ggtaaacaaa cagcctcaaa ggcgtatcgg
    31021 gtaggttact gcgacgggat cgaccagata ttgctgccag gtattaccgc aaagatgttt
    31081 tgtggcgtcc cggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc
    31141 aatacattga acaacagcaa acacctggtc agatgaaaaa acgagcctta tatccccgcc
    31201 ctgaaggacg ggcttttacg gtgcactgga taagcctgga gattatctac acctgcatgg
    31261 acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa agtattagag
    31321 ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaatagaac
    31381 taatatcaag cggcgctata ttgcgtatta aataaaacca cctcaattac attaataaat
    31441 ctaaataaat atcatgcaaa taatttccat ggttattttc aggctattta aattaaaaaa
    31501 ataaattatg agtcaatcat aaataacaac ctttaatata atttgactac caaatttata
    31561 ttcgttcaca aaatagtcat gcattgtgtg caatagaaac agttcagata aaaataggga
    31621 tctacttcac aatttaaatg agaaactaaa acttacatct tgaaataatc tcattgattg
    31681 gatgaatatt tgtagtgcag tgacatcatt tttaaataat agttcaaata aaagggctca
    31741 cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg atggcgatgt
    31801 ctgctcaggc agcagagatt tacaataaag acagtaacaa gctggatctg tacggaaaag
    31861 ttaatgctaa gcactacttc tcttctaacg atgcagatga tggcgacaca acttatgttc
    31921 gtctgggctt caaaggcgaa actcaaatca acgatcaact gactggtttc ggtcagtggg
    31981 aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc ttccaaagat aaaacccgtc
    32041 ttgccttcgc tggcctgaaa ttcggtgact acggctccat cgattatggc cgtaactacg
    32101 gtgtagcata cgatatcggt gcatggaccg acgttctgcc agaattcggt ggcgatacct
    32161 ggacccaaac agatgtgttc atgactggtc gcaccacagg tgttgcaacc tatcgtaaca
    32221 acgacttctt tggtctggtt gatggtctga actttgctgc tcagtatcag ggtaaaaatg
    32281 accgcactga cgtaactgaa gctaacggtg atggtttcgg tttctccact acttatgagt
    32341 atgaaggatt cggtgtaggt gcaacctatg ctaaatctga ccgcactaat gatcaggtta
    32401 tctacggtaa caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg gcagctggtc
    32461 tgaaatatga tgcgaacaac atctatctgg ctaccaccta ttctgaaacc cagaacatga
    32521 ctgtttttgg taataaccat attgccaaca aagcacaaaa cttcgaagta gttgcacaat
    32581 atcagttcga cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa ggaaaagact
    32641 tgggtgcgtg gggtgatcag gacctgattg aatatattga tgtaggtgca acctattact
    32701 tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa cctgattgat aagagcgatt
    32761 tcacgaaagc atctggcgtt gctaccgatg atatcgttgc tgtaggattg gtttaccagt
    32821 tctaatctga ttactcaaag atatgttgtg ggaggctttg cctccccaac atataagtgg
    32881 cgctctcaag ccactttcct tagaagcact atcttgcttc tgactatata aattaccctt
    32941 tattttctgg gacgtttcaa cgccccattt ttaataatac tcagtgaaca attaacgtat
    33001 caattagatt taataacaac gatatccgta cctaacctga tatctaatgt cataagcatc
    33061 cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca catagcccaa
    33121 acgccggatg caaaaaaaat cacacgtgca tctctgaaat tgtgatggag gaagtgagta
    33181 tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga ttgttcaggc
    33241 tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc tgttgctcct
    33301 taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt atattaaaac
    33361 gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc ctggcaatct
    33421 cgctgatggg ttttactgtc gtttgtatag caggacaacg cattatttaa cctcacgtcg
    33481 tccaatatac tttgaaaact tattccctcg cttgccagac ggcgatgcaa tgtggaaaca
    33541 gaaatgtaga gatatctcgc taccttgttt gctgtccatt ttgttccagg ttcggatagc
    33601 attaggttat aacaacgact tattaatgat tgtttactat ctgataaaag taaacaatta
    33661 acatgatgtg ctcctaatga aagtagaacg cccattgcta agtgttcctg aatttgcgtt
    33721 gagaagcctc gggaaataga tgtttttagt tgctcccaac aatatattaa ctcggggttc
    33781 tgaggtaagc agaaacctgt cgtgttacga atttgatcag ttatcgcata aaatttttga
    33841 aacttttcaa ttagatcaat gggataataa agcatttctg caagataaag acctgcttct
    33901 ggataattca caatataaaa ttcatatcca cagggaaata atattatctg attattatca
    33961 acagttagac tatgcgtctc ccaattgata actttttttc cctgacggat acgacataaa
    34021 gctggcataa gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg tatttcttca
    34081 atctttaggt tagacttatc tcttgccagc atactcttac cctattttat ctcataaact
    34141 ggtgttatct cagcggttgc aattttattg gcattaagca tataaccaac taacgctccg
    34201 ctggaattag aatccacagg aatcttatca gtttttagag cccatacttt aaactggtaa
    34261 tgatggggtt tatctccttt gggaggacat gctccaccaa atcctgcata accaaaatca
    34321 tttcggcctt gaacagcacc agtcggaagt tttgtaccat cacgtcttcc tgcatcagtg
    34381 ggcaaatatg ttacagttgc tggaatatta gcaacagtcc agtgccacca tccactgcct
    34441 gtaggagcat ctggatcata cacagttacg gcaaagcttt tggtgccttc aggagcacca
    34501 gaccaggtta atgagggcga tgtattacca ccttcacacc caaatccaga aaatacatga
    34561 gacgttgtaa gttgctctcc tgtttttatt tcattactag tgacctgaaa tgctgcggcc
    34621 tgcgcagaaa atgttatgaa tgccaataca cttgaaacga taagtttttt cataaaaacc
    34681 tcattgttgt gacctatcgt tattttattt gatgtgcctt gatctcatta tgaataaagg
    34741 cgcaatgatc gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt agccataata
    34801 tgcacaaatc agattcctgt tatctcaagg agaattattt tttaatgcgg tgcacttcgt
    34861 gacaacaagt taatgactaa cacaccggca caaatcaaca tcatgcctat gatagctggc
    34921 aggtccagtc gttgaccaaa aaatccccag gaaagcaaac taatcagaac gataccgact
    34981 cctgaccaga tagcataagc aatccctgta ggtatataag ccagcgtctg agctaataac
    35041 cagaatgatg cacaatagca aataattgtg ccaacagagg gccataaccg tgtaaacccc
    35101 tctgaaaact tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc accaagataa
    35161 atataagggt tcatagcata ttctttcctg ttcatactgg agagaattgt actacagttt
    35221 gaacacaact caccgttttc atcctggtgt atgtgtatcc aaggggctct ggcgtggcaa
    35281 gagataaagg caggaacaat aatgatcaga aggaacccca ggctaacgaa tgagttcact
    35341 cttcctggga gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg tggatggcag
    35401 aaaacaacca ggatcaggta tccattttaa accagaaatt gagtgagttt gccccatcca
    35461 tgccccacgc tgtgggatcg gatataatta aacaagcata gtcaacaggt tacacaacct
    35521 acacctgcat ggacatcact tcctgatacg ccgccaccag cttattacgc acctgaatcc
    35581 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt agcgccacgc
    35641 cgggttcacc gagggtgaat ttttctgcct gcgtgcgggc gactgtttgt gtatcgctta
    35701 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgc ggcagtgatt
    35761 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
    35821 gtatcgctga cattttcgtc tcccggataa tttctggtaa caaagcctac cagtaagtca
    35881 ataagacaaa ggcgctaaat agcaacaaaa aaactggttt attggcggat agaaaaaacg
    35941 aaagcacaaa taatgggagc gtcaattttt cgagtttgct gacccgagag tgagtcttgt
    36001 tccactttgc caataacgcc gtccataatc agccacgagg tgcgcgatga atgcgactgc
    36061 agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga aaattccatt
    36121 gattgttacc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt gggcgaaagc
    36181 ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg caattgtcag
    36241 ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg ctattgaggt
    36301 tccggcagat aaagttcacg aactgcacct gcgcctggcg caacaaggtt tgcccaaagg
    36361 cggcgcggtc ggtttcgaac tgctcgatca ggaaaagttt ggtatcagcc agttcagcga
    36421 acaggtgaat tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg
    36481 cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga
    36541 acaaaaatcc ccttctgcat cggtgacggt aaatctgtta cccggccgcg cactcgatga
    36601 agggcaaatt agcgctattg tacatctggt ttccagcgcg gttgctggtc tgccgcctgg
    36661 aaacgtcacg ctggtggatc agggcgggca tctgttaacc cagtccaata ctagcgggcg
    36721 cgatcttaat gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta ttcagcagcg
    36781 tattgaagcg atcctgtcac ctattgttgg taacggtaat attcacgcct aggtcacggc
    36841 gcaactggac ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga
    36901 atctcatgcg gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta
    36961 tccgggcggc gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat
    37021 cagcacgcct ccgacaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag
    37081 tgggccgcgt agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg
    37141 tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtgaatta
    37201 caaaaccttg ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga
    37261 agctctgacc cgcgaggcga tgggcttttc tgaaaaacgc ggtgattcgc tcaatgttgt
    37321 taactcgccg ttcaatagca gtgacgaaag cggcggagca ctgccgtttt ggcaacagca
    37381 agtgtttatc gatcagttgc tggctgccgg tcgctggttg ctggttgctg gtactgctgg
    37441 tggcgtggtt gctgtggcgg aaagcggtac gcccgcagct aacacgtcgc gccgaggcgg
    37501 tgaagactgt gcagcaacag gcgcaggccc acgaggaagt ggaagatgcg gtggaagtcc
    37561 gtctgagcaa agacgaacaa ctccaacaac ggcgcgctaa ccaacgactg ggggcagaag
    37621 tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca
    37681 ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa aagcgtcatc
    37741 ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct ctcccagcgc
    37801 gaagtgcaaa ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa caagcagcta
    37861 accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgctgcact gaatatcaac
    37921 gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaagaacg tgccgccagc
    37981 ctgctggaag atattctcga aacccgcgat accgccagcg gtattgaaac gctcaacttt
    38041 atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat tatcgccacc
    38101 attctggtgc atctgaagcg cgcctaggcc gccgatattc tggcgttgtt cgatgaacgt
    38161 ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc agccgcgctg
    38221 gccgaactga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa gcgcagcaaa
    38281 atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaacgca gcaggaagaa
    38341 gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat catcgacgag
    38401 atgttcctgt tcgagaatct ggtggatgtc gatgatcgca gcattcagcg tctgttgcag
    38461 gaagtggatt ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc actgcgcgag
    38521 aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga tctcgccaac
    38581 cgtggtccgg tgcgtctgtc gcaggtcgaa aacgaacaga aagcgattct gctgattgtg
    38641 cgccgtcttg ccgaaactgg cgagatggtg attggcagcg gcgaggatac ctatgtctga
    38701 taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccaccgg cagagtttgt
    38761 gccaatggtc gagtcggaag aaaccattat tgaagaggcc gaacccagcc ttgagcagca
    38821 actggcgcaa ctgcaaatgc aggcccacga gcagggttat caggcgggga ttgccgaagg
    38881 tcgccagcaa ggtcacgaac agggctatca ggaaggactg gcccaggggc tggaacaagg
    38941 tctggcagag gcgaaggctc aacaagcgcc aattcatgcc cggatgcagc aactggtcag
    39001 cgaatttcaa actacccttg atgcgcttga tagtgtgata gcttcgcgcc tgatgcagat
    39061 ggcgctggag gcagcacgtc aggtgatcgg tcagacacca acggtggata actcggcact
    39121 gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggga aaccacagct
    39181 gcgcgtacac ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa ctttaagttt
    39241 gcatggctgg cgcttgcggg gcgatcccac cctccaccct ggcggatgta aagtctccgc
    39301 cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct gccgtctggc
    39361 agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac gctggataac
    39421 tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt aacccgcgct
    39481 accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac ctgtgtcatt
    39541 gagcgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg ctttaacggt
    39601 caacggctgt ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg cgcgcgtgta
    39661 tatgccaaaa acatttcggc agaagggctg caaagcggca agcagttgcc gctcggtccg
    39721 gcgttattag gtcgtgttct ggacggtagc ggtaaaccgc tcgatggcct gcccttcccc
    39781 gatacgacgg aaaccggtgc gctgattacc ccgccattta acccgttgca acgtacaccg
    39841 attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac cgtcggacgc
    39901 gggcagcgta tggggctgtt tgccgggtcg ggcgttggta aaagtgtgct gctggggatg
    39961 atggcacgct acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga acgtgggcgc
    40021 gaagtaaaag attttattga gaatatcctc ggtgccgaag gacgtgcacg ctcagtggtg
    40081 attgccgctc cggcggatgt ttctccgctc ctgcgaatgc aaggtgccgc ctatgccacg
    40141 cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat ggactccctc
    40201 acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc acctgccact
    40261 aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg tgccggtaat
    40321 ggcattagcg gcggcggctc gattaccgcg ttttataccg tgctcaccga aggcgatgac
    40381 cagcaggacc ccattgctga ctccgcgcgg gcaatcctcg acggtcacat tgtgctgtct
    40441 cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc gatcagtcgc
    40501 gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt caaacagctg
    40561 ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc caaaggcagc
    40621 gatccgatgc tcgataaagc catcgccctg tggccgcagc tggagggcta tttgcaacaa
    40681 ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg tattttcccg
    40741 acagtgtcat aacccaggag ataacggcag atggcggaag aacagctcaa aatgttgatc
    40801 gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc
    40861 aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat
    40921 cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa
    40981 aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg
    41041 cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg
    41101 aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat
    41161 tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat
    41221 tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtagca acagataagc
    41281 ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt
    41341 tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac
    41401 cgttaaccac cgctcagacg atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag
    41461 acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga
    41521 tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg
    41581 cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg
    41641 atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta
    41701 aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc
    41761 cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgta ccgctgggtt
    41821 ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc caggggcaac
    41881 aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag
    41941 tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc
    42001 tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc
    42061 aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc
    42121 aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg
    42181 ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac
    42241 gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga
    42301 taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat
    42361 aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc
    42421 ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc
    42481 gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt
    42541 gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac
    42601 ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc
    42661 acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat
    42721 taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt
    42781 gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg
    42841 aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta
    42901 gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac
    42961 gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt
    43021 tcaacctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccgtacc
    43081 atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc
    43141 tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc
    43201 tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg
    43261 aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga
    43321 aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca
    43381 atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca
    43441 acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat
    43501 tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc
    43561 tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc
    43621 tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg
    43681 atcgcatcat cgcccatgtt gacggcgtcc cggttctgac cagtcagtac ggtactctca
    43741 acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg
    43801 aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga
    43861 cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga
    43921 cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct
    43981 gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa
    44041 agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc
    44101 gctggatatt ctgatcaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga
    44161 taaatatggc gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag
    44221 ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca
    44281 ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg
    44341 ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc
    44401 gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg
    44461 ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca
    44521 ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt
    44581 ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta
    44641 cgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcggtggac
    44701 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    44761 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    44821 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    44881 tgaccttttt tattatgtcg ccagtgatca acaaaattta tgtcgatgcg taccagccat
    44941 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    45001 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    45061 ccggcccgtt gcaggggcct gaagctgtgc cgatgcgcat tttgctcccg gcctacgtga
    45121 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    45181 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    45241 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    45301 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    45361 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    45421 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    45481 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    45541 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    45601 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    45661 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    45721 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    45781 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    45841 cgtacagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    45901 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttgt
    45961 cgatcctggc agccatctca atatgcccgt tttagcgcgc atcatggata tgctggcatt
    46021 actgctgttc ctgacattta acggtcattt atggttgatt tcactgctgg tcgatacctt
    46081 tcacaccctg ccgattggta gcgaaccgtt gaacagcaat gcgtttctgg cacccaccaa
    46141 agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgtt
    46201 gctgaccctg aatctggcat taggtttact gaatcgtatg gccccgcaat tatccatttt
    46261 tgttattgga tttccattaa ctctgactgt cggcatctct ttaatggcgg cattaatgcc
    46321 gttaattgcg cctttttgcg aacatttatt cagtgaaatt tttaatttgc tggctgatat
    46381 tattagtgaa ttaccattaa tataattccg taacgtttat catgctaccc taaggattat
    46441 ccgaaaaata atatctgcga acatcttcca ggatactcct gtagcgaaat atttgtttta
    46501 agctcactca catatcgcaa catttacttt aagacaattc caggcaaatt atacaacact
    46561 ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt
    46621 gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga
    46681 gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc
    46741 gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat
    46801 gaccttgcca tagcttgtga ttcacagcgt ccttcagtgg tgtttattaa tgaggactgt
    46861 ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca acatcccaat
    46921 acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc
    46981 agaaaaaatt tattgatcag ttctaaatcg attaaaccgg aatctctcga cgatatcctt
    47041 ggcgatattc tgaaaaaaga gacaacgata acctcgtttt taaatatgcc gacgttatca
    47101 ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg caggtcaggg aaccattcaa
    47161 atctccgacc aaatgaatat caaagccaag accgtttcat cgcataaagg taatattaaa
    47221 cgtaagatca aaacgcataa taaacaggtt atctaccatg tcgtccgact gacggataat
    47281 gtgactaatg gtatttttgt caacatgcgc taacacattc tgactggtgg tttcccacca
    47341 gtcaggctga ataagattac tctgctttct caacaaagat accgtcctga tgccctgctt
    47401 cattaaagaa ccagatgccg agcgggtagt cttccagcgc aaccaggtac attgtgcctt
    47461 cactaaactc ctcaactgcc agtaccactc caggacgacg cggaccgcca tccgttttga
    47521 ctgttacccg atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac
    47581 aatttcgttt ttctggcagc gccaagcgcg cgcgagtgct gattttctcg acggtctata
    47641 cttaagagat gccaggcgga cttaacgact ggcggcaaca acagagtaac ggttgcgagg
    47701 aaagatgatg aaaaccgcaa aagtgtacag cgataccgca aaacgtgagg tcagcgtcga
    47761 tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa agcgaagttc atcgcagcca
    47821 gaacgattct gaacacgtta gcgtcgatgg acgtgaatat catacatggc gtgaattggc
    47881 ggatgccttc gaactggata ttcatgactt cagcgtctct gaagtgaatc gttgaatgca
    47941 caataaaaaa atcccgaccc tgagggtgtc gggatgaaac ttgcttaagc aagaagcact
    48001 taaaaaattc gttacaccag gaaatctgat gtgttcatca ccttatctgc aatttttttc
    48061 gctgacaaga aaatattcgc gctatgaatg attaacgcta cttatgaata gaaatgtgac
    48121 gcgcgtcacc ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa atcatctttg
    48181 attatttgtg ctgttttgag aatcatcctg gtaaggggag tactgcgcga ccatgttttc
    48241 aattcaacaa ccactactgg tttttagcga tcttgatggc accctgctgg acagtcatag
    48301 ttatgactgg caaccggcag ccccctggct cagccgttta cacgaagcca atattcccgt
    48361 cattctctgt agcagtaaaa ctttagcgga aatgctgtac ttgcaaaaaa tgttggggct
    48421 acaaggctta ccgctgattg cagagaatgg cgcagtgatc cagcttgctg aacaatggca
    48481 ggatatagac ggttttccac gcatcatctc aggtattagc catggcgaaa tctgccaggt
    48541 tttaaatacg ctacgcgaga aagaacattt taaattcacg acttttgatg atgtcgacga
    48601 tgcaaccatt gccgaatgga cgggattaag ccgtagctag gcggcgctga cgcagctgca
    48661 tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag cgtatggcac aatttatcgc
    48721 tcgtctgaac gaactgggct tgcagtttat gcagggtgcg cgcttctggc acgtactgga
    48781 tgcctctgcc ggaaaagatc aggctgccaa ctggattatc gcgacctatc aacaattgtc
    48841 aggcagacgc ccaaccacac ttggcctggg cgatgggcca aacgatgcgc ctttactgga
    48901 ggtaatggat tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtac atctgcatga
    48961 tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct ggcgtgaagg
    49021 gctggaccat tttttctccg cccgttaagc gttatcgctc gcgaataccc gattgcgccc
    49081 ggcctgtttt gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt caaaatcata
    49141 atcaccggtt tcctcgctgc tacttacccc cagcgaggca ctgatgcgta tcgttgtact
    49201 cttggcgatc agcatctctt tttcatttaa cttcaggcga atacgttctg cgacctccgc
    49261 agcctgcgtc agattcgcgc ctggcagaat cacacaaaac tcctcaccac cgacccgccc
    49321 ggcaacgtcc tgcgcacgca aggaactgct aattaatccg gcagcatgag aaagaacacg
    49381 gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa gatcgacctg
    49441 aatgacagaa aaaggatgtt ggtgcgtctg acacaattta gcgagcggac gggctttttc
    49501 gaacagtgcg ccacggttat ataaacgcgt taaggtgtcg tgccacgcct gccactgcaa
    49561 cgagctttgc agaacataca tgttgctgac catccggcga atcacatacc aggagaggag
    49621 taacatggtg gtaaagagcg cccacagcag ggttaatgca atgctgatac tgccgaaatc
    49681 gccgcgcacg ccttcgctta gcgtatggac gcgcgccagc acaccgtcga aatggtccag
    49741 acgttcccag ctaacatagc gactgtccat acgaatgccg ccccgcgtgt catgttccat
    49801 tgcctgcgcc agcaaggcca gttcacgagg atcaaaaata ttccctgttg gatggtcagg
    49861 attggaagag gtcaaaaatc tcagcttact gtcatagagc tgatactcac catcgaggtt
    49921 tttatcgatg gcgtttctta aaaattgctg catagtacgc acgggaatac tcatccccag
    49981 cacgccatac cagtagttat tactgtctac cggaacactg acggtaacct gcggttcagt
    50041 attgctggcg tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg
    50101 ttgcgaatgg ccgataaacc agggttgggt gacatagccg taataacgcg ttggtacatt
    50161 gcgcgtaaac aaggtcggct gcgtcgaaac gtaaaatcca gcacgcgaga catacatcgc
    50221 ctgttcaacc atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa cttccagtgc
    50281 agcggtaatt tcattgtcga ggctttcatt ttcgcgagac aggaaattcc cctcgctgac
    50341 taatgcatcc gatacgccat tgactggcag ggtgcgccgt cggttgagtt cgatttgcca
    50401 agcgtgctcg tcgcgatgct gttcgaactc ggttacggta ttacgcagag aggtgaaatc
    50461 cagtggcgcc acgagagctt cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt
    50521 atactgcaaa tgcttatcga gcgcgttggc tacgttttcc agatgattac gctagctgga
    50581 gatataggca tcttccagca ccaccacttc acgccaggtg agcaaggtgg aaaaaagcaa
    50641 taccacgata aagcagagat taacgacatg accgggcccc aggcggcgtg cgagtttttt
    50701 caaccagctc tggttttcca tttttgtctc gtgctgcacc ctgacgactc ccaacctgct
    50761 tttttatgat tctggcataa cccggcaata gcgtaccgca aaacagcgat aattttataa
    50821 tggtggcatg ataagacgcg actatcattc attgcaggca ttggaacacc aggccggatg
    50881 tgatccggcc tggtgttaat gatacccttt caaacttctt ccgcagaacg actcccctcc
    50941 cccggaacaa gatcggtttc atgaaaagct tcccgcttga ccccgtaacc gtcataccaa
    51001 cgacactcaa ccataccgct ggagtatcca gtgacaatca tccgcgggcc gccctcttta
    51061 accgtaactt cctcactaac cataaagctc atactcgcct ccttttttcg agtgaaactt
    51121 gttcacctta gttgaagatg gcgaattttg cataatagcc agtgcgagta ttaatgtgcc
    51181 tgaccgcgca ttttcgctac tgctttcact cccagcacca cgatgccgcc gatgataaat
    51241 ccaagaatca gatttaaaac agtcggtaat atcatcgcca ccactgcact ttgctgcccg
    51301 gcgatatgtt caatagcgtg atgcagcggc gcaataccat gtaccacaat cccgccgccg
    51361 acgaggaaca tcgccagcgt gccgacaatc gataatgctt tcatcagcca aggcgcgata
    51421 atcaataacc ctttacctaa tgcctgcatc agcgcgctag atttttccgc cagccaatac
    51481 cctaggtcat caatcttaac gataacccct accagaccgt agacgcccac ggtcactacc
    51541 agcgcgatgc ctgaaagcac cagcacctga ttaagcaacg gcgcctctgc cacaatcccc
    51601 agcgtgatgg cgacgatttc cgcagacaaa ataaaatcgg tacgaatcgc ccctttaatt
    51661 ttgtcctttt caaacttcag cggatcctgc gccgccagct tctccagacg ctgctggctc
    51721 tgcgctggat cttctttatg tttacgcgcc tccagcatat gcagcacttt ctctacccct
    51781 tcaaagcaga gaaacgcgcc accaatcatc aacagtggcg taatcgccca cgggataaac
    51841 gcactgatga tcagcgccag cggcaccaga atcactttat tgatcagcga tcctttcgcc
    51901 acgccccaga ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg
    51961 agcgataaat catcccctaa tacaccggcg gttttcttcg ccgccagttt gcccatcaca
    52021 gagatatcgt ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact actcccggcc
    52081 aaaagcattc cctctcattt ttattgttga agggtgtaag tatgaagcaa aaacggcagc
    52141 tctgatactt gcttcacagg tcaacattta tttaacaatc aagtgcaatt atatctctcg
    52201 ccagcagaat gattttcacg tttactatga accacctttt tatttccgct gtgagggatt
    52261 atgcgtttcc gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg
    52321 ggctcaacct attttgtcat tcggattggc gtgggaagct ggcctccgtt aatgatggcg
    52381 ggcgttcgat tccaggcagc cggtatttta ttgctggcat ttttgctact gcgcggacac
    52441 aaacttcccc cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt attgctggct
    52501 gtcggtaatg gcatggtgac ggttgccgaa catcaaaatg ttccttccgg catcgccgcc
    52561 gtagtgattg caaccgtgcc cctctttacc ctgtgcttca gtcgcctgtt tggcattaaa
    52621 acgagcaaac tggaatgggt gggtattgcc attgggcttg ccggaatcat catgctcaat
    52681 agcggtggaa atttaagcgg caatccgtgg ggcgcgattc tgattttaat cggctcgatt
    52741 agctgggcgt ttggctcagt ttatggctcg cgcattacct tacctgtagg gatgatggcg
    52801 ggtgcgattg agatgctggc ggcaggcgtg gtgttaatga tcgcatcgat gattgcgggt
    52861 gaaaaactga cggcgctccc ttccctgtca ggcttccttg cggtcggcta tctggcgctg
    52921 tttggttcga ttatcgccat taacgcttat atgtatttaa tccgtaatgt cagtccggct
    52981 ctcgccacca gctacgccta cgttaacccg gtggtcgcgg tcttgctggg tacgggactg
    53041 ggtggcgaaa cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt tttcgcggtg
    53101 gtactggtca cgttgggaaa atatctcttc ccggccaaac ccgttgtcgc gccagttatt
    53161 caggatgcat caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc tggcaccttc
    53221 gccgcagatc cactcttcca gacgctcggt aagcgccgca tccgtcagct tctcgcgcct
    53281 acgtaacgca cactcccaga caatcagtac gcgccagccg agctcctgca agcgactgat
    53341 atcgcggcga tcgcgttcaa catttttacc tatcttctcc agccagaact cagttcgagt
    53401 cgcaggcact ttaaacagat agcagtgatg atgatgccag aagcagccgt gggtaaatat
    53461 cacgcagcga tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac
    53521 gcgaaacgca aggccctgcc cggttaacaa actggcgagg cgcttctcaa tcgccgtatc
    53581 acgcgtggca atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa cgtcgaccat
    53641 gttgtgcctc ttgctgacgc aacgccaccg cctgtttgat ttttggctca agcagttttg
    53701 ccaccgcggc aaagaccggc acgaccaccg agttgccgaa ctggcgatag gcctgagtgt
    53761 ccgaaaccgg aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc
    53821 attcacgagg cgttaaccga cgtggacgat gttgctgatt cagcggatcg tcaaagtctt
    53881 tctcacccgt ggccatatcc caaccgcgat cgattaaaat ttccgcgcca tctttgtaat
    53941 aacgagcaga cagcgtacgg gtgacgcttt gcggattgtt cggataaacc attccataac
    54001 cgaagccgtt accgcgcgcc tgatgttttt tcgcatatcg atagaggtac ttccacagca
    54061 ccggcgtcag gatatatttc gcctcaacca tcgggtccag cagctgcgcc agcgtcactc
    54121 gctgtgcagg gaaacattcg ctgatatcac gcagagtaaa atcggctttc agattcagat
    54181 cgcgacgaaa acccaccagc acgatgcgtt cacggtgctg cggcaggaaa tgtttaccat
    54241 cgatgatttt cggatcgtcc ggcccgttat cttctgcatc ggcaacgtca tagcccagtt
    54301 cgtccagcgt ctgcatgatg atgcggaacg tttttccctt atcgtgactc ttcaggtttt
    54361 tgacgttttc gagcacaaac atcgccggac gacgcgcgtc gataatgcgc accacatcaa
    54421 agaacagcgt gccctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt
    54481 tcgatacgcc agccagcgaa aatggctgac aagggaaacc ggccagtaaa acatcgtgtt
    54541 caggaatgtg ttgacgaata tgttccgccg ccgcctcatc actcacgcct tcttgatgac
    54601 tgagggtgat gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg caataatggt
    54661 tggctttata agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg cactgtccac
    54721 caatcgattc aaaaccacga cggatgccgc caattccggc aaatagatcg ataaagcgaa
    54781 acgcataatg cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga tgggcaaact
    54841 ctttctcact taaacggtgc catgccgagt cattcgccag cgcacgtttt aaaattgccg
    54901 cgctccagtg attctcaccc acaccattaa gctgcgccac cagcatttta acatcataaa
    54961 tttgcagcag tttctccagc attgcctgtg cggcattccc ggtgctgtat gaatcggtta
    55021 ctgatatatt ttcctgcata gatttaaccg gccatctgag atggccgaca ggttaacata
    55081 attactgccg tttagggcca cgcgctcagc cagcgggtga aaacgcatct atcaccttgt
    55141 gatcgatacc ttcattctcc cccgccagtt cggcaccgag cttcgccatc aaacagcgcc
    55201 aagctcaatg cccctgaaac ggcaaatacg cgcgccaggc caatcgcccc cagggcttcc
    55261 agccgatccg catcctggac gattttagct tccgttgtta agggggcgat ttgcgcgctg
    55321 aaactgtgag cggcaatggc atgacaaacg gcctcaattt tctccgccgg aaattgcacg
    55381 aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga acgctgccgt
    55441 tgcggatgat ttttcgccag gctcacgata tcgtgaaaat aacaggcggt taaaatcacc
    55501 agcatatcga catcgtcatc tgccgccagt ttctgtgccg tagcccagac acggcgaaaa
    55561 tgacacaggt catgcgccgc gtcctgatgt tgatgatggt ttttcaacca gttctcgaac
    55621 tgtgcctgcc agtgttgtaa ctccatacgc gctccgttgc caaagagtcg caaccttagc
    55681 agtttttatt catagcgtct gcaacgtgcg ggacgaggtc gggtgtacca tgcaatgccg
    55741 cctaacaagg caccaaccag ggccataaac agaaatccga ccaatgctcc taaccacttc
    55801 cccgcggtag atcctaaatc actttcaaat cccgacacgg gggcatcggg gatccggctc
    55861 atcacccaga tgtatcgcgc cattgcccag acgaaatagc cacaccatgt atagaaagcc
    55921 c
//
LOCUS       DAJTRB010000014        55075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTRB010000014 DAJTRB010000000
VERSION     DAJTRB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.014"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(117..1490)
                     /gene="argH"
                     /locus_tag="OO330_000945"
     CDS             complement(117..1490)
                     /gene="argH"
                     /locus_tag="OO330_000945"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5881905.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1551..2327)
                     /gene="argB"
                     /locus_tag="OO330_000946"
     CDS             complement(1551..2327)
                     /gene="argB"
                     /locus_tag="OO330_000946"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5881906.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2335..3339)
                     /gene="argC"
                     /locus_tag="OO330_000947"
     CDS             complement(2335..3339)
                     /gene="argC"
                     /locus_tag="OO330_000947"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5881907.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3493..4644
                     /gene="argE"
                     /locus_tag="OO330_000948"
     CDS             3493..4644
                     /gene="argE"
                     /locus_tag="OO330_000948"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5881908.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4996..7647
                     /gene="ppc"
                     /locus_tag="OO330_000949"
     CDS             4996..7647
                     /gene="ppc"
                     /locus_tag="OO330_000949"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5881909.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7830..9563
                     /locus_tag="OO330_000950"
     CDS             7830..9563
                     /locus_tag="OO330_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5881910.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9712..10563
                     /locus_tag="OO330_000951"
     CDS             9712..10563
                     /locus_tag="OO330_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5881911.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10550..10891)
                     /locus_tag="OO330_000952"
     CDS             complement(10550..10891)
                     /locus_tag="OO330_000952"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5881912.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10893..11771)
                     /locus_tag="OO330_000953"
     CDS             complement(10893..11771)
                     /locus_tag="OO330_000953"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5881913.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11737..14034)
                     /locus_tag="OO330_000954"
                     /pseudo
     CDS             complement(11737..14034)
                     /locus_tag="OO330_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            complement(14085..14405)
                     /locus_tag="OO330_000955"
     CDS             complement(14085..14405)
                     /locus_tag="OO330_000955"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5881914.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14420..15499)
                     /locus_tag="OO330_000956"
     CDS             complement(14420..15499)
                     /locus_tag="OO330_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5881915.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15808..16911
                     /locus_tag="OO330_000957"
     CDS             15808..16911
                     /locus_tag="OO330_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5881916.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16912..18309
                     /gene="ptsP"
                     /locus_tag="OO330_000958"
     CDS             16912..18309
                     /gene="ptsP"
                     /locus_tag="OO330_000958"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5881917.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18321..18983
                     /gene="fsa"
                     /locus_tag="OO330_000959"
                     /pseudo
     CDS             18321..18983
                     /gene="fsa"
                     /locus_tag="OO330_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18994..20095
                     /gene="gldA"
                     /locus_tag="OO330_000960"
                     /pseudo
     CDS             18994..20095
                     /gene="gldA"
                     /locus_tag="OO330_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20370..20987
                     /locus_tag="OO330_000961"
     CDS             20370..20987
                     /locus_tag="OO330_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5881918.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSSHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21014..21919)
                     /gene="yijE"
                     /locus_tag="OO330_000962"
     CDS             complement(21014..21919)
                     /gene="yijE"
                     /locus_tag="OO330_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5881919.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22013..24193)
                     /gene="katG"
                     /locus_tag="OO330_000963"
     CDS             complement(22013..24193)
                     /gene="katG"
                     /locus_tag="OO330_000963"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5881920.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPPSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24522..25412)
                     /gene="metF"
                     /locus_tag="OO330_000964"
     CDS             complement(24522..25412)
                     /gene="metF"
                     /locus_tag="OO330_000964"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5881921.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25761..28193)
                     /gene="metL"
                     /locus_tag="OO330_000965"
     CDS             complement(25761..28193)
                     /gene="metL"
                     /locus_tag="OO330_000965"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5881922.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28196..29356)
                     /gene="metB"
                     /locus_tag="OO330_000966"
     CDS             complement(28196..29356)
                     /gene="metB"
                     /locus_tag="OO330_000966"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5881923.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29633..29950
                     /gene="metJ"
                     /locus_tag="OO330_000967"
     CDS             29633..29950
                     /gene="metJ"
                     /locus_tag="OO330_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5881924.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30134..30742
                     /locus_tag="OO330_000968"
     CDS             30134..30742
                     /locus_tag="OO330_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5881925.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30803..31015)
                     /gene="rpmE"
                     /locus_tag="OO330_000969"
     CDS             complement(30803..31015)
                     /gene="rpmE"
                     /locus_tag="OO330_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5881926.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31218..33416
                     /gene="priA"
                     /locus_tag="OO330_000970"
     CDS             31218..33416
                     /gene="priA"
                     /locus_tag="OO330_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5881927.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33572..34597
                     /gene="cytR"
                     /locus_tag="OO330_000971"
     CDS             33572..34597
                     /gene="cytR"
                     /locus_tag="OO330_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5881928.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34689..35648
                     /gene="ftsN"
                     /locus_tag="OO330_000972"
     CDS             34689..35648
                     /gene="ftsN"
                     /locus_tag="OO330_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5881929.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35741..36271
                     /gene="hslV"
                     /locus_tag="OO330_000973"
     CDS             35741..36271
                     /gene="hslV"
                     /locus_tag="OO330_000973"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5881930.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36281..37612
                     /gene="hslU"
                     /locus_tag="OO330_000974"
     CDS             36281..37612
                     /gene="hslU"
                     /locus_tag="OO330_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5881931.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37679..38605
                     /gene="menA"
                     /locus_tag="OO330_000975"
     CDS             37679..38605
                     /gene="menA"
                     /locus_tag="OO330_000975"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5881932.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38698..39183
                     /gene="rraA"
                     /locus_tag="OO330_000976"
     CDS             38698..39183
                     /gene="rraA"
                     /locus_tag="OO330_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5881933.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39268..39513)
                     /gene="zapB"
                     /locus_tag="OO330_000977"
     CDS             complement(39268..39513)
                     /gene="zapB"
                     /locus_tag="OO330_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5881934.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39939..40784
                     /gene="glpF"
                     /locus_tag="OO330_000978"
                     /pseudo
     CDS             39939..40784
                     /gene="glpF"
                     /locus_tag="OO330_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40824..42314
                     /gene="glpK"
                     /locus_tag="OO330_000979"
     CDS             40824..42314
                     /gene="glpK"
                     /locus_tag="OO330_000979"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5881935.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42544..43554
                     /gene="glpX"
                     /locus_tag="OO330_000980"
     CDS             42544..43554
                     /gene="glpX"
                     /locus_tag="OO330_000980"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5881936.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43651..44397
                     /gene="fpr"
                     /locus_tag="OO330_000981"
     CDS             43651..44397
                     /gene="fpr"
                     /locus_tag="OO330_000981"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5881937.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44402..44830)
                     /gene="uspD"
                     /locus_tag="OO330_000982"
     CDS             complement(44402..44830)
                     /gene="uspD"
                     /locus_tag="OO330_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5881938.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44857..45156)
                     /locus_tag="OO330_000983"
     CDS             complement(44857..45156)
                     /locus_tag="OO330_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5881939.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45368..45808)
                     /locus_tag="OO330_000984"
                     /pseudo
     CDS             complement(45368..45808)
                     /locus_tag="OO330_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45909..46508
                     /locus_tag="OO330_000985"
     CDS             45909..46508
                     /locus_tag="OO330_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5881940.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46616..47383
                     /gene="tpiA"
                     /locus_tag="OO330_000986"
     CDS             46616..47383
                     /gene="tpiA"
                     /locus_tag="OO330_000986"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5881941.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47438..48193)
                     /gene="cdh"
                     /locus_tag="OO330_000987"
     CDS             complement(47438..48193)
                     /gene="cdh"
                     /locus_tag="OO330_000987"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5881942.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48300..49289)
                     /gene="sbp"
                     /locus_tag="OO330_000988"
     CDS             complement(48300..49289)
                     /gene="sbp"
                     /locus_tag="OO330_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5881943.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49609..50571)
                     /gene="pfkA"
                     /locus_tag="OO330_000989"
     CDS             complement(49609..50571)
                     /gene="pfkA"
                     /locus_tag="OO330_000989"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5881944.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50752..51654)
                     /gene="fieF"
                     /locus_tag="OO330_000990"
     CDS             complement(50752..51654)
                     /gene="fieF"
                     /locus_tag="OO330_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5881945.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51802..52302)
                     /gene="cpxP"
                     /locus_tag="OO330_000991"
     CDS             complement(51802..52302)
                     /gene="cpxP"
                     /locus_tag="OO330_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5881946.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52452..53150
                     /gene="cpxR"
                     /locus_tag="OO330_000992"
     CDS             52452..53150
                     /gene="cpxR"
                     /locus_tag="OO330_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5881947.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53147..54520
                     /gene="cpxA"
                     /locus_tag="OO330_000993"
     CDS             53147..54520
                     /gene="cpxA"
                     /locus_tag="OO330_000993"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5881948.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54626..>55075)
                     /gene="yiiM"
                     /locus_tag="OO330_000994"
     CDS             complement(54626..>55075)
                     /gene="yiiM"
                     /locus_tag="OO330_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5881949.1"
                     /translation="AELFVAPAFGENLSTDGLTESNVHMGDIFRWGEALIQVSQPRSP
                     CYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPLELVSRVSDVTVQEAAAI
                     AWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFSRRLWGK"
BASE COUNT        13424 a        14917 c        14087 g        12647 t
ORIGIN      
        1 accagaatgc tgacaaattt ctgcctgatg cggcgtaaac gccttatccg cctacgaagt
       61 gtcgcacttt gtaggcgtgg taaactcccc attttcaggg gagcaatata aacaaattac
      121 tctaaccgag cctgcgcaaa agcaatcgcc tgcgccacct gctgcggtga gacgccgcct
      181 tttgccgcac gcttttcgag gcacgattgt agcgacagaa tcggatagac atcttcgcca
      241 atcacctgac tgaatttctg caactcactg agtggcagat cttccagcgg tttgccctga
      301 cgaatggctt ccaccaccgc ttcacccaca atatggtgcg cctcgcggaa aggtacgcct
      361 ttcgccacca ggtaatccgc cagttcagtg gcgttcgcat acccctgctg cgccgcttcc
      421 tgacaacgcg gacgtttcac ctgaatgccg tccagtacca gcgccgccat atgcaggcag
      481 tccagccagg tatcgagcgc gtcgaacaga ccttctttgt cttcctgcat atctttgttg
      541 taagccagcg gcaaaccttt cagcgtcatc atcatgccgg ttaacgcacc ctgcacccgg
      601 ccgcatttac cgcgaatcag ctccagcgca tccgggtttt tcttctgcgg cattaatgat
      661 gaaccggaag tcacgcggtc cgaaagctcc acaaaccccg cttcgccggt gttaaagaaa
      721 atcagatctt cagcaaaacg cgacagatgc accatgccga tagcggcagc agaaagcagt
      781 tccaacacat ggtcacggtc agaaacgctg tccagactgt tacgggtcgc cgaagcaaag
      841 cccagccagc ctgctaactg ttcacggtcg atttcatagg ccgttcccgc cagcgcgcca
      901 cagcctaatg ggctgacatc cagacgctta agcgcatcct gcaaacggct ttcatcacgc
      961 gccagcatct caacataggc caggcaccag tgcgcgaacg tcaccggctg ggcgcgttgc
     1021 aggtgagtgt aacctggcat taccgcgtcc tgattgtttt gtgcggtttc caccagcgcc
     1081 gattgcagct gccggttagc cgtcagtaac tcgctaacgg tatctttgca ccacagtttc
     1141 aggtcagtcg ctacctgatc attacggcta cgcccggtat gcagcttttt gcctaactgg
     1201 cccactttgt cgatcagttt gccttccacc cagctatgga tatcttcggc gtcgctttca
     1261 aggatttgtt gtggcctggc gcgaacatct tccagcaaca cgttcagcgc ctcttccagt
     1321 tgcgcctgct cttctgcggt taacacgccg accgttacca aggctttgga ccaggccaca
     1381 gagccaacaa tatcctgctc cgccagacgg taatcaaagc gcagtgagtc gttgaattgt
     1441 ttgaaccgtt gatctgctgc ctgggtaaaa cgcccgcccc aaagtgccat aactctgttt
     1501 ccttattttg aaattcaatg ccggaaagct gcgcctccgg ctaacaaaac ttaagctaaa
     1561 atccgcgtac ccatcggcat accgttaaac agtgccggaa gctgctccgc ataacgccaa
     1621 gaagcgatat ctaccggacg gcccagcgtg cgggccgcat ccagcgccgc gttcactttc
     1681 actatcatgc cgtcagtaat aatgccctgc tcaatcagtt gttctgcttt cgcggcggtc
     1741 atttcggcaa tgcgttgccc tttgccgtcg agaatgccgc tgacgtcgga gagcaaaatc
     1801 agatccgcgc ccagcgttgc cgccagcgcc gttgccgcct ggtcggcatt gacgttcatc
     1861 agttgccctt cgtctgttac gccaatggag ctgaccaccg gcagataacc gttctccagc
     1921 aaggagttga taagcttagg cgaacctggc tgcgccagtc caacatgacc taactcttca
     1981 tcaagctggg tcactttgac gctgtcgccg tcaccgagaa acaaacctac ggccgcaatc
     2041 tgatgtttct tcgcccatgc caacagggtt ttatttgccg ttcccgccag tgctccggtg
     2101 ataatgtcta tctgatcagc aggcgtcacc cgcaggccgt ttttcttttt caccggcaga
     2161 ttcagccctt tcatcagctc atccaccacg caaccgccgc cgtgcacaat caccagcgga
     2221 cgctgatgtg actcacgata attcaccaat gcgctaaaca gacgttccag cgcctcttca
     2281 ctatccagca gtacgccgcc cagtttgata attaatggat tcatcattgc acccttaaat
     2341 aagagactgc gtttccgcat agccgaaacg aatattggcg cactgtaccg cttgtgccgc
     2401 cgcgcctttc agtaagttgt cttcggtcgc cacaataatc agatgctcgc cctgaacggc
     2461 aaacccgata tcgcaaaatg gcagcccaac gacatttttc agcgccggaa cgcctttgtc
     2521 atacagccgc accagcggtt tatgggcata cgcctgctgt aacgcttgcg cgacttgcgc
     2581 ctgggtcaca cccgatttca ggcggcaggt aatggtttcg agaatgccgc gcgggaaatt
     2641 gcccagatgt ggggtgaaga taacgtcagc accgaggtgt gtggcgatct ctggttgatg
     2701 gcgatgagta aagacgccat acggttgcag gctaacttca caaaaactat ttgaaatcgc
     2761 tgccttacgt cctgcaccgc tcacgccgct ggtggcgttg atcaccggcc actgattgag
     2821 gtcaagaaga tcggcatcaa tcaacggttt cagcgccagc tgtgccgccg tcggataaca
     2881 gcctggcacc gcaatcaaat tcgcttcttt taatttatta ccgcaccact ccgccagacc
     2941 gtaggctgcc tgttccagca gttccgggta ttgatgggta aagccgtaat atttttcata
     3001 gaaggtggcg tcgttaacac gaaacgcgcc ggaaaggtcg aacaccacgc agcccgcttc
     3061 aagaaactgc ggcgctaaat cgtggctaac ttcatgggcg gtggcgagaa acactacgtc
     3121 cacccctggg ctaaactcgc tgatatccga catcggctgc aacggcagct caacgatgcc
     3181 ttttagctgc ggatgcaaat cggagattaa ctttcccgca tcattgcttt gcgctgaaac
     3241 agtcaaagcg gttatgttca tatgcggatg gcgatttaca taggtcacta gctctgcgcc
     3301 agcgtagccg ctggcaccca caatcagcgt attcaacatc ggggctattc accttcttat
     3361 gtctggttgc caggttaaac gtaaaacatt caccttacgg ctggtgggtt ttattacgct
     3421 caacgttagt gtatttttat tcataaatac tgcatgaata ttgatactat catgaccaga
     3481 ggtgtgtcaa caatgaaaaa caaattaccg ccatttatcg agatttaccg cgctctgatt
     3541 gccacacctt caataagcgc tacggaagag gcactcgatc aaagcaatgc agatttaatc
     3601 actctgctgg cggactggtt taaagatttg ggcttcaatg tggaagtgca gcctgttcca
     3661 ggaactcgca acaaatttaa tatgctggcc agttacggac agggggctgg cggcttgttg
     3721 ctggcggggc ataccgatac ggtgccattt gatgacggtc gctggacgcg cgatccgttt
     3781 acactgacgg agcatgacgg caagctttac ggcttaggca ccgccgacat gaaaggcttt
     3841 tttgcgttta tccttgatgc gctacgcgat gtcgacgtca cgaaactgaa aaaaccgctc
     3901 tacattctgg cgactgctga tgaagaaacc agtatggccg gagcgcgtta ttttgccgaa
     3961 actaccgccc tgcgcccgga ttgcgccatc attggcgaac cgacgtcact acaaccggta
     4021 cgcgcacata aaggtcatat ctctaacgcc atccgtattc agggccagtc ggggcactcc
     4081 agcgatccag cacgcggaat taacgctatc gaactaatgc acgacgccat cgggcatatt
     4141 ttgcaattgc gcgataacct gaaagaacgt tatcactacg aagcatttac cgtgccatac
     4201 cctacgctca acctcgggca tattcacggt ggcgacgctt ctaaccgtat ttgcgcttgc
     4261 tgtgagttgc atatggatat tcgtccgcag cctggcatga cactcaatga acttaatggt
     4321 ttgctcaacg atgcattggc tccggtgagc gaacgctggc cgggtcgtct gacggtcgac
     4381 gagctgcatc cgccgatccc tggctatgaa tgcccaccga atcatcaact ggttgaagtg
     4441 gttgagaaat tgctcggagc aaaaaccgaa gtggtgaatt actgtaccga agcgccgttt
     4501 attcaaacgt tatgcccgac gctggtgctc ggtccgggtt ccatcaatca agctcatcag
     4561 cctgatgaat atctggagac gcggtttatt aaacccaccc gtgagttgat tatccaggta
     4621 attcaccatt tttgctggca ttaaaacgta ggccggataa ggcgctcgcg ccgcatccgg
     4681 caccgttgcc aaactccagt gccgcaataa tgtcggatgc gatgcttgcg catcttatcc
     4741 gacctacacc tttggtgtta tgtatggcga ttttttaaca tttccataag ttacgcttat
     4801 ttaaagcgtc gtgaatttaa tggcgtaaat tcctgctatt tattcgtttg ctgaagcgat
     4861 ttcgcagcat ttgacgtcac cgcttttacg tggctttata aaagacgacg aaaagcaaag
     4921 cccgagcata ttcgcgccaa tgcgacgtga aggatacagg gctatcaaac gataagatgg
     4981 ggtgtctggg gtaatatgaa cgaacaatat tccgcattgc gtagtaatgt cagtatgctc
     5041 ggcaaagtgc tgggagaaac catcaaggat gcgttgggag aacacattct tgaacgcgta
     5101 gaaactatcc gtaagttgtc gaaatcttca cgcgctggca atgatgctaa ccgtcaggag
     5161 ttgctcacca ccttacaaaa tttgtcgaac gacgagctgc tgcccgttgc gcgtgcgttt
     5221 agtcagttcc tgaacctggc caacaccgcc gagcaatacc acagcatttc gccgaaaggc
     5281 gaagctgcca gcaacccgga agtgatcgcc cgcaccctgc gtaaactgaa aaaccagccg
     5341 gaactgagcg aagacaccat caaaaaagca gtggaatcgc tgtcgctgga actggtcctc
     5401 acggctcacc caaccgaaat tacccgtcgt acactgatcc acaaaatggt ggaagtgaac
     5461 gcctgtttaa aacagctcga taacaaagat atcgctgact acgaacgcaa ccagctgatg
     5521 cgtcgcctgc gccagttgat cgcccagtca tggcataccg atgaaatccg taagctgcgt
     5581 ccaagcccgg tagatgaagc caaatggggc tttgccgtag tagaaaacag cctgtggcaa
     5641 ggcgtaccga attacctgcg cgaactgaac gaacaactgg aagagaatct cggctacaaa
     5701 ctgcccgtcg aatttgttcc ggtacgtttt acctcgtgga tgggcggtga ccgcgacggc
     5761 aacccgaacg tcactgccga tatcacccgc cacgtcctgc tgctcagccg ctggaaagcc
     5821 accgatctgt tcctgaaaga tattcaggtt ctggtttctg aactgtcgat ggttgaagcg
     5881 acccctgaac tgctggcgct ggttggcgaa gaaggtgccg cagaaccgta tcgctatctg
     5941 atgaaaaacc tgcgttctcg cctgatggcg acacaggcat ggctggaagc gcgcctgaaa
     6001 ggcgaagaac tgccaaaacc agaagggctg ctgacacaaa acgaagaact gtgggaaccg
     6061 ctctacgctt gctaccagtc acttcaggcg tgtggcatgg gtattatcgc caacggcgat
     6121 ctgctcgaca ccctgcgccg cgtgaaatgt ttcggcgtac cgctggtccg tattgatatc
     6181 cgtcaggaga gcacccgcca taccgaagcg ctgggcgagc tgacccgcta cctcggtatc
     6241 ggcgactacg aaagctggtc agaggccgac aaacaggcgt tcctgatccg cgaactgaac
     6301 tccaaacgtc cgcttctgcc gcgcaactgg caaccaagcg ccgaaacgcg cgaagtgctc
     6361 gatacctgcc aggtgattgc cgaagcgccg caaggctcca ttgccgccta cgtgatctcg
     6421 atggcgaaaa cgccgtccga cgtactggct gtccacctgc tgctgaaaga agcgggtatc
     6481 gggtttgcga tgccggttgc tccgctgttt gaaaccctcg atgacctgaa caacgccaac
     6541 gatgtcatga cccagctgct caatatcgac tggtatcgcg gcctgattca gggcaaacag
     6601 atggtgatga ttggctattc cgactcagca aaagatgcgg gcgtgatggc agcttcctgg
     6661 gcgcaatatc aggcacagga tgcattaatc aaaacctgcg aaaaagcggg tattgagctg
     6721 acgttgttcc acggtcgcgg cggttccatt ggtcgcggcg gcgcacctgc ccatgcggcg
     6781 ctgctgtcac aaccgccagg aagcctgaaa ggtggcctgc gcgtgactga acagggcgag
     6841 atgatccgct ttaaatatgg tctgccagaa atcaccgtca gcagcctgtc gctttacacc
     6901 ggggcgattc tggaagccaa cctgctgcca ccacctgagc cgaaagagag ctggcgtcgc
     6961 attatggatg aactgtcagt catctcctgc gatctctacc gtggctacgt acgtgaaaac
     7021 aaagattttg tgccttactt ccgctccgct acgccggaac aagaattggg caaactgccg
     7081 ttgggttcac gtccggcgaa acgtcgccca accggcggcg tcgagtcgct gcgcgccata
     7141 ccgtggattt ttgcctggac gcaaaaccgc ctgatgctcc ccgcctggct gggtgcaggt
     7201 acggcgctgc aaaaagtggt cgaagatggc aaacagagcg agctggaagc catgtgccgc
     7261 gattggccat tcttctcgac gcgtctcggc atgctggaga tggtcttcgc caaagcagac
     7321 ctgtggttgg cggaatacta tgatcaacgt ctggtagaca aagcactgtg gccgttaggt
     7381 aaagagttac gcaacctgca agaagaagac atcaaagtgg tgctggcgat tgccaacgat
     7441 tcccatctga tggccgatct gccgtggatt gcagagtcta ttcagctacg gaatatttac
     7501 accgacccgc tgaacgtatt gcaggccgag ttgctgcacc gctcccgcca ggcagaaaaa
     7561 gaaggccagg aaccggatcc tcgcgtcgaa caagcgttaa tggtcactat tgccgggatt
     7621 gcggcaggta tgcgtaatac cggctaatct tcctcttctg caaaccctcg tgcttttgcg
     7681 cgagggtttt ctgaaatact tctgttctaa caccctcgtt ttcaatatat ttctgtctgc
     7741 attttattca aattctgaat ataccttcag atatccttaa ggaattgtcg ttacattcgg
     7801 cgatattttt ttcaagacag gttcttacta tgcattccac agaagtccag gctaaacccc
     7861 tttctagctg gaaagccctg ggttgggcac tgctctactt ttggtttttc tctactctgc
     7921 tacaggccat tatttacatc agtggttata gtggcaccaa cggcattcgc gactcgctgt
     7981 tattcagctc gctgtggttg atcccggtat tcctctttcc gaagcggatt aaaattattg
     8041 ccgcagtgat cggcgtggtg ctatggacgg cctctctggc ggcgctgtgc tactacgtca
     8101 tctacgggca ggagttctcg cagagcgttc tgtttgtgat gttcgaaacc aacaccaacg
     8161 aagccagcga gtatttaagc cagtatttca gcctgaaaat tgtgcttatc gcgctggcct
     8221 atacggcggt ggcagttctg ctgtggacac gcctgcgccc ggtctatatt ccaaagccgt
     8281 ggcgttatgt tgtctctttt gccctgcttt atggcttgat tctgcatccg atcgccatga
     8341 atacgtttat caaaaacaag ccgtttgaga aaacgttgga taacctagcc tcgcgtatgg
     8401 agcctgccgc accgtggcaa ttcctgaccg gctattatca gtatcgtcag caactaaact
     8461 cgctaacaaa gttactgaat gaaaataatg ccttgccgcc actggctaat ttcaaagatg
     8521 aatcgggtaa cgaaccgcgc actttagtgc tggtgattgg cgagtcgacc cagcgcggac
     8581 gcatgagtct gtacggttat ccgcgtgaaa ccacgccgga gctggatgcg ctgcataaaa
     8641 ccgatccgaa tctgaccgtg tttaataacg tagttacgtc tcgtccgtac accattgaaa
     8701 tcctgcaaca ggcgctgacc tttgccaatg aaaagaaccc ggatctgtat ctgacgcagc
     8761 cgtcgctgat gaacatgatg aaacaggcgg gttataaaac cttctggatc accaaccagc
     8821 agacgatgac cgcccgcaat accatgctga cggtattttc gcgccagacc gacaagcagt
     8881 actacatgaa ccagcaacgt acgcagagtg cgcgtgaata cgacaccaac gtgctgaagc
     8941 cgttccagga tgtgctgaat gaccctgcgc cgaagaaact gatcatcgtt catctgctgg
     9001 gtacgcatat caaatacaaa taccgctacc cggaaaatca gggcaagttt gatggcaata
     9061 ccgatcatgt tccgccagga ttaagcgcag aagagctgga atcatataac gattatgaca
     9121 acgctaacct gtataacgat catgtggttg ccagcctgat taaagacttt aaagcggcag
     9181 acccgaacgg attcctggtt tacttctctg accacggtga agaggtttac gacacgccgc
     9241 cgcacaaaac tcaggggcgt aacgaagaca acccgacgcg ccacatgtac accattccgt
     9301 tcctgctgtg gacgtcggaa aaatggcaag cgactcatcc ccgtgatttc tcacaggatg
     9361 tcgatcgtaa atacagcctg gcggaactga tccacacctg gtcagatttg gcgggcttat
     9421 cttacgacgg ttacgatcca acccgttcag tggtgaatcc gcagttcaaa gaaactaccc
     9481 gctggattgg taacccgtac aagaaaaacg cgctgatcga ttacgacact ctgccgtatg
     9541 gcgatcaggt gggtaatcag tagatgacct ttgtatcgcg tctggcgcta tttgccggat
     9601 gcgatactgc cgcaatgttt acatcgaact cccctcaggc cacacttttc aggtggcctt
     9661 ttttaataat gccagttact gttttcccgt ctacatacct aagggaataa catgtatcac
     9721 gacgtcagct atctgctctc ccgcctgatc aacggcccgc tgtccctgcg ccagatttac
     9781 tttgccagca gtaacggccc agtgcccgat cttgcgtatc aggtcgattt tccacggctg
     9841 gaaattgtgc tggaaggtga gtttgttgat accggcgctg gagcaacgtt agttcccggc
     9901 gatgtgctgt acgttgccgc tggtggctgg aatttcccac aatggaaaac acccgctacc
     9961 acctttagcg tgctgtttgg caaacagcaa ctcggcttca gcgtcgtgca atgggatggc
    10021 aaacaatatc aaaatctggc gaagcaacac gtcgcccgac gcggcccacg cataggttct
    10081 tttctgctac aaacgctcaa tgaaatgcaa atgcagccgc aggagcagca aacggcaagg
    10141 cttatcgtag ccagcctgct tagccactgc cgcgatttgc tcggcagcca aatccagacc
    10201 gcctcacgca gccaggcact atttgaagct attcgcgatt atatcgacga acgctatgcc
    10261 tccgcgctta cccgcgaatc tgtcgcacag gcattctata tttcaccaaa ctatctctcg
    10321 cacctgtttc aaaaaacggg ggccattggt tttaacgaat acctgaatca cacgcgactg
    10381 gagcacgcta agacgttatt gaaaggctat gacctgaagg taaaagaagt ggcgcacgcc
    10441 tgcggctttg tcgacagcaa ctacttctgc cgtctgtttc gtaaaaacac cgaacactcg
    10501 ccgtcggaat atcgccggca gtaccacagc caactgacag aaaaaccgac tactccagaa
    10561 taagatgggt ttgctgcggc gcagaaagca ctttgcgcac cgcgctcatt acccgctgcg
    10621 gctcacgcag gaatgcgtag atgctgcatt gcacatagcg gcaatgttca aatcgctcgg
    10681 caccggcaag ctcgatatcc gtaatcaaca gagcaacatc cgcccgacgg atatcctcgt
    10741 ccgctaaacg attctccgtt cccagcgcgc cctgagtttc aatgctgacg ccccacttct
    10801 ctaactggca caacttttcc agccgttccg ccgccatata agtatgcgcc acgccgctta
    10861 cgcaggcggt tactgccacc aggtatgcca ttttaacctc ccacggtaac ctgaaatccg
    10921 gcccgttctg ccatttcgcg catcgttgtc acatcggctg acgacggcgc aggcacctct
    10981 ttcatcgacc atgttttccc cagcaggcgg tatttcggtt cgccgtactg atgaaacggt
    11041 aacagatgga tctgcctgat tttcagcggg atcagtacat ccagcgcctg ctgcatattc
    11101 tcccggctga gcgtgaaacc agggatcaac ggtaaacgcg ggatcacgtt gacaccctca
    11161 ctcaccagca aacgcagatt ctccagcacg cgtggcaggt tcatcttcac cacatcccgc
    11221 gcctgagtcg cgtccataat ttttaaatcg aacagcactt catcgcacaa tttcgccagc
    11281 ggtaacagct tgcttgctgg cgcgtctccg gcagtttcaa tggcgcatga gacaccccac
    11341 agccgcagtc gctgtaaaaa acgggtagca aactccgcct gcattaacac ttcgccgcca
    11401 gaaagcgtga cgccgccgcc ggacgtgcga aagaaaatgt catctttcat cacttcccgt
    11461 tccagagcgt caaggctgat atcgcgacca atccgttcaa acgccccaga ggggcattca
    11521 tccgcatcac gcaaacattt cgcacagtgc agacatttcg cctctctgcg taccgtctgg
    11581 attttgccgg agatcgactc cggattagca caccacgggc aaagatgcgg acagccttta
    11641 aaaaagacca ccgtacgaat gccctcgccg tcattcagtg aataacgctg aatgttgaaa
    11701 atgcgcgcca catcatcgcg gcgcgtttcc acaacgttac agctgatgcg ctgtccggcg
    11761 gatgatgtca tcctggatct ccttcgacag ttcgacaaag aaggcgctgt atccggcaac
    11821 gcgcaccacc agcccggcat aatcttgtgg gcgctgttgc gcttcccgca acgtgtcggc
    11881 gttcaccacg ttaaactgaa tatgctgcaa cttaagctgg gtaaacaccc gtaagaagtc
    11941 ggccagtttg cgtaatcccg cttcaccttc cagggtcgcc ggagtgaatt tcacgttcag
    12001 caacgtaccg ttagacagca gcgtattatc gagcttactg actgacttca gtaccgccgt
    12061 tggcccttgt gcgtcctgac ccagcatagg tgacaagccg ccgtctgcca gttgttcccc
    12121 ggcaaaacga ccgtctggcg tcgcgccaac caccgatccc aacggaacgt gagcagaaac
    12181 ggtatacgat cccggcgtga agtaaccgcc gcgcgggttc tggtattttt ccacttcttt
    12241 gcagtagtgg cgcaacagtt cggcgctaat gttatccacc tcgtcgatat cgttaccgta
    12301 tttctcaaag cggttaatta agcgagcgcg gactttttcg ccttctggcg ttgcaaagtt
    12361 ggcttttaat accgacagca attcgtcaaa acttaaacgc tgttgctcaa aaaccatccc
    12421 tttcaacgca tggagagaat cgctcaggtt ggcgatacca attccctgta cgccggagaa
    12481 gttataacgc gcgccgccat cggtaatatc gcgacctttt tccagacaat cgctgataaa
    12541 agacgagagc agcggtacag gtgcccagtc gcgatggccg atatcacaaa tattgctgcc
    12601 ctcaaccatc agggtgatgt agtggctgat cttggcgcgg atctgctcca gtaaaccttc
    12661 ataagtcagc gcagcattgc cttcattttc atgcaggcag atttccatca ctttcagcag
    12721 attaaacatc gcgatgtcat gcaagccgta ggttctgccg ggaatagata attccacaca
    12781 gcccactacg gaatagtcgc gcgcatcttc cagcgaaacg ccacggttga ggaacgctgg
    12841 caccaccact tcatcgttaa agatttgcgg aataccggtg ccgaggcgaa tggtttcggc
    12901 ggttttcatc aggaacggcg tgtcgatcaa ggcgttagtg cgcacgccga ggttcggttg
    12961 cggtaattgc acgctttgat aggcgtcaag gcaaaggaac gaaagcacgt tcaccgcgct
    13021 acgtccgttc tcggttaacc cgccgagcag tgcggtatag ccggtcggga aaccggcgaa
    13081 ataacgcgcg ctactggtgg agcgcaacag cacgatgtcg ttgcatttca cccataaaga
    13141 ttcgagcagt tctttcagga acgccggatc ttcgccctgg gttaaagatg cctgatagaa
    13201 cggcaacata tactggtcga agcgccctaa agacagcgaa ctggcgttgg attcgtattg
    13261 cagaatgatg ttcatgtacc agaataactg gcacgcctgc cagaacgtct gcggcttatg
    13321 ttgcgcgtta tggcgggaga tctccgcaat agtcagcagc tcttcgcgac gctgggcatc
    13381 tgtgcagttt gccgccatcg tttccgccag ttcggcgtaa cgcaaaatgt gtttctgcga
    13441 ggcttccagc agtaacaacg cggcctgata aaagtgattc tccggctgtt gctgacaatg
    13501 ttgctgcatc tatgccacca gctcacccag accgtgattc aacaggcgtg ggtaatcaat
    13561 aataatgtgc ccctggcctt tatccgtctg gttgatgctg aaaatctgcg tgttggtcgc
    13621 ggcttttact tcatctgtca tctgcccgtt gatgaaatct ttcatcgaac gtttttccca
    13681 gtacgggaac aactcttcac ggtagatacg tttatcttct tcgctgatag caaagcggtc
    13741 ctgcggacgc gtcgggaatt gatccagctc tttcagcagc cagtaagggt ccatttccgg
    13801 cgacataatc ccggcgcgcg gttttacggt gcggttaccg gcaatcagtt cttcatcacg
    13861 aatcgaaatt tcaacatgtt caaggatata cgctgttgct ttcgcccggc gcaatatcac
    13921 cggttcgcct tcggtttgcc gatggctggc ggtataaagc agcgcccgct ccagcgagat
    13981 ttcacgggta ttggcaaaca gtgcagtttt gaggcgagag atacgattcg tcatgaggaa
    14041 ctcctgtggg aaagcgaacc cgcccttgcg agcgggagaa gaacttaagc ggtttgcgcc
    14101 aggtgagctt caattttgct cataatggcg tcagcacgtt ttactgcatc gctgatgttg
    14161 acgcgcacga tggtcttgcc agcaaaacgc tcttcaaact tgatgccgat atctttggtc
    14221 agaatcacca tatcagcgct cgccacgtct tccgcagtca gttcattttc cagaccaatt
    14281 gacccctggg tttctacttt cacttcccag cctttcgctt tcgcggcact ttccagcgcc
    14341 tctgctgcca tataggtatg ggcaacacct gaaggacatg cggttactgc aataattttc
    14401 gtcatggctc gttcctcaat taattaattt caaaatccaa atccaggtcg tcttcttttt
    14461 catcagtcga agacccattt ttacgcgcca gacttttcag cacgttcaca caaacagccg
    14521 tcaccactgc tccgacagcc actgctgcga tataacccaa cttaccttca accaccggca
    14581 gcacaatcag accaccccag cctgcatagc actgcgcacc gaccagcgcc gcagttactg
    14641 caccacaaac tgaaccgacc ataatggaag gaatgacacg cagcggatcg gcagcggcga
    14701 aaggaatcgc cccttcagta acgccaacac agcccatcac cagtgccgct ttgccggttt
    14761 cgcgctcttc tgcggagaaa tttttacgac caatcagcgt cgccagcccc atccccagcg
    14821 gcgggataca aatacccacg gcagcaatgg ccaccacggt ataaacaccc tgagcaacgc
    14881 aaatcagcat gaaggcatag gccactttgt taaccggacc gcccatatcg aacgccagca
    14941 tcagccccat gatcaccgcc agcataacaa tgctgccctg ctgcatcccc tgaagccact
    15001 gagtcaggct gttggtcaac gcccctacag gctcgcccaa gccccacatc atgatgcctg
    15061 cggtaatcag tgtgccgatg ataggaataa tgaagatggg catcaccgag cgcagaactt
    15121 tatgcaccgg aattttcttc aggtaatgca ccacgatgcc gccgataatc ccggcgatca
    15181 gcgcaccaaa gaagcccgca ccaaagctgt taccaaccca ggcaccgata gcgcacggag
    15241 ccagcgcaga acgctctgca atggagtaac caatgtaagc ggcgaggaaa ggcaccatca
    15301 gcgtcaaacc tgcaacgccg atatcaaaca gttttttcaa attcggatcg gctacggcat
    15361 ccggcactgc gcctttgcca tacaacatga cggaaaccgc cagcaaaata ccgcccgata
    15421 ccacgaaggg aatcatgtgt gaaacgcccg tcattaaatg ctgacgggta tttttcagga
    15481 tctgcaccaa ctcattcata agctgctccc gggcttgcaa aagcccatgt ccatattttt
    15541 gatggaggaa acaataggca atcgcaggcg gagcaaacag tggataaaaa ggccatttac
    15601 tggacttttg taatatcagt acaaaaatgc gatccgcctc ataacttgcg ataatccgga
    15661 agcatgccgc aaaacggcgt cttgtgagac gcgtcgcaaa ttgacggtta cattacattt
    15721 gtccagcttt tggcagattt gtcacatggc aggccgttac cgtgcgctct tatcctgtaa
    15781 acagaaaacc gtaccgggag aaaggcaatg gccctgattg tggaatttat ttgtgagcta
    15841 cctaacggcg tacatgcgcg tccggcaagc cacgttgaaa cgctgtgtaa tactttttca
    15901 tctcaaattg agtggcataa cctgcgtact gaccgtaaag gcaacgccaa aagcgctctg
    15961 gcgctgattg gcaccgatac gctggtgggt gataactgcc agctactgat ttccggggcc
    16021 gacgaacagg aagggcacca gcgtttaagc caatggctgc gcgatgaatt cccccactgc
    16081 gatgcgccgc tggcggaagt taaatctgac gaactggaac cactgccggt ttcactgacc
    16141 aatctgaatc cgcaaattat ccgcgcccgc accgtgtgca gcggtagtgc aggcggcatt
    16201 ctgacgccga tctcttcttt agatctcaat gcgctgagta atcttcccgc agccaaagac
    16261 gttgacgccg agcaatccgc actggaaaac ggcctgacgc tggtactgaa aaacattgag
    16321 tttcgtctgc tggatagcga cggtgctacc agcgcgattc tggaagcgca ccgatccctg
    16381 gctggcgata cttccctgca cgaacattta ctggcaggcg tcagcgccgg attaagctgc
    16441 gccgaagcga ttgtcgccag tgcgaatcac ttttgcgaag agtttgcccg ttccagcagc
    16501 agatacctgc aagaacgtgc cctggacgta cgcgacgtct gcttccagtt actccagcaa
    16561 atctacggtg agcaacgctt cccggcaccg ggcaaactga cgcagtccgc catttgtatg
    16621 gctgatgaac tgacccccag ccagttcctc gaactggata aaaatcacct caaaggattg
    16681 ttactgaaaa gcggcggcac cacctcacat acggtgatcc ttgcccgttc gttcaacatt
    16741 ccgacgctgg ttggtgtgga tattgatgcc ctcactccgt ggcagcatca aacgatttat
    16801 atcgacggca acgccggggc gattgtggtt gagccaggag aagccgtagc tcgttattat
    16861 cagcaagaag cccgcgtaca ggacgccctg cgtgagcaac agcgtgtcta gctgacccaa
    16921 caagcccgta ccgctgacgg tatccgcatt gaaattgccg ctaacatcgc tcactccgtg
    16981 gaagcgcagg ccgcattcgg caatggtgcg gaaggcgttg gtttgttccg cactgaaatg
    17041 ctctatatgg atcgcaccag tgcaccgggc gaaagcgagc tgtacaacat tttttgtcag
    17101 gcgctggagt ctgccaacgg acgcagcatt attgtgcgca ctatggacat tggcggcgac
    17161 aaacccgttg attatctaaa cattcccgca gaggcaaacc cgttcctcgg ttatcgcgcc
    17221 gtgcgtattt atgaagaata cgcgtcgtta tttaccacac aactacgctc gatcctgcgc
    17281 gcctccgctc acggcagcct gaaaatcatg atcccgatga tctcctcaat ggaagagatc
    17341 ttatgggtga aagaaaaact ggcagaagcc aaacagcaac tacgtaacga acacattccg
    17401 tttgatgaga agatccagct cggcattatg ctggaagtac cgtcggtgat gttcatcatc
    17461 gatcaatgct gcgaagagat tgatttcttt agtattggta gtaatgacct gacgcaatat
    17521 ctgctggcag tggatcgcga taacgctaag gttactcgtc actacaacag cctgaatccg
    17581 gcattcttgc gggcgctcga ttacgacgtg caggcggtgc atcgccaggg taaatggatt
    17641 ggtctgtgcg gtgagctggg agcgaaaggt tccgtgctgc cgttgctggt cggcttaggg
    17701 ctggatgaac tcagcatgag cgcaccatca attccggcgg cgaaagcgcg gatggcgcaa
    17761 cttgatagcc gtgagtgccg caagttgctc aaccaggcaa tggcctgccg tacttcgctg
    17821 gaagtggaac acctgctggc gcaattccgc atgacccaac aggacgcacc gctggtcacc
    17881 gccgagtgca tcacgctgga aagtgactgg cgcagcaaag aagaagtgct caaaggcatg
    17941 accgataacc tgctgctggc gggccgctgc cgctatccgc gtaaactgga agccgactta
    18001 tgggcgcgcg aggccgtttt ctctaccggt ctgggcttta gttttgccat tccacacagc
    18061 aaatcagaac acattgagca atccaccatc agcgtggcgc gtctgcaagc gccggtgcgc
    18121 tggggcgatg atgaagcgca attcatcatt atgttaaccc tgaacaaaca cgctgcgggc
    18181 gatcagcaca tgcgcatttt ctcgcgcctc gctcgccgca tcatgcacga agaattccgt
    18241 aacgcgctgg ttaacgccgc ctctgccgac gctatcgcca gcctgctgca acatgaactg
    18301 gaactgtaaa aggaaacatc atggaactgt atctggacac cgctaacgtc gcagaagtcg
    18361 aacgtctggc acgcatattc ccgattgccg gggtgacaac taacccgagc attatcgctg
    18421 ccagcaagga gtccatctgg gaagtgctgc cgcgtctgca aaaagcgatt ggtgatgaag
    18481 gcattctgtt tgctcagacc atgagccgcg acgcgcaggg gatggtggaa gaagcgaagc
    18541 gcctgcgcga cgctattccg ggtattgtgg tgaaaatccc ggtgacttcc gaaggtctgg
    18601 cagcaattaa aatactgaaa aaatagggta ttactacact tggcaccgct gtatatagcg
    18661 ccgcagaagg gttattagcc gcactggcag gggcaaaata cgttgctccg tatgttaacc
    18721 gcgtagatgc ccagggcggg gacggcattc gtacggttca ggagctgcaa acgctgttag
    18781 aaatgcacgc gccagaaagc atggtgctgg cagccagctt taaaacgccg cgtcaggcgc
    18841 tggactgttt actggcagga tgtgaatcca tcaccctgcc cttagatgta gcgcaacaaa
    18901 tgctcaacac ccctgcggta gagtcagcta tagagaagtt cgaacacgac tggaatgccg
    18961 catttggcac tactcatctc taaaggagca attatggacc gcattattca atcaccgggt
    19021 aaatacatcc agggcgctga tgtgattaat cgtctgggcg aatacctgaa gccgctggca
    19081 gaacgctggt tagtggtggg tgacaaattt gttttaggtt ttgctcaatc cactgtcgag
    19141 aaaagcttta aagatgctgg actggtagta gaaattacgc cgtttggcgg tgaatgttcg
    19201 caaaatgaga tcgaccgtct gcgtggcatc gcggagactg cgcagtgtgg cgcaattctc
    19261 ggtatcggtg gcggaaaaac cctcgatact gccaaagcac tggcacattt catgggtgtt
    19321 ccggtagcga tcgcaccgac tatcgcctcg accgatgcac cgtgcagcgc attgtctgtt
    19381 atctacaccg atgagggtga gtttgaccgc tatctgctgt tgccaaataa cccgaatatg
    19441 gtcattgtcg acaccaaaat cgtcgcgggc gcacctgcac gtctgttagc agcgggtatc
    19501 ggcgatgcgc tggcaacctg gtttgaagcg cgtgcttgct ctcgtagcgg cgcgaccacc
    19561 atggcgggcg gcaagtgcac ccaggcggcg ctggcactgg ctgaactgtg ctacaacacc
    19621 ctgctggaag aaggcgaaaa agcgatgctt gccgccgaac agcatgtagt aaccccggcg
    19681 ctggagcgcg tgattgaagc gaacacctat ttgagcggcg ttggttttga aagcggcggc
    19741 tggcggcggc tcacgcggtg cataacggcc tgaccgctat cccggacgcg catcactatt
    19801 atcacggtga aaagtggcat ttggtacgct gacgcagctg gtactggaaa acgcgccggt
    19861 ggaggaaatc gaaaccgtag ctgcacttag ccatgcggta ggtttgccaa taactctcgc
    19921 tcaactggtt attaaagaag atgtcccggc gaaaatgcgc attgttgcaa aagcatcttg
    19981 tgcagaaggt gaaaccatcc acaacatgcc tggcggcgcg acgccagatc aggtttacgc
    20041 cgcactgctg gtagctgacc agtacggtca gcgtttcctg caagagtggg aataacctac
    20101 tccaaactcc cggcttgtcc gggagtttga acgcaaaatt gcctgatgcg ctacgcttat
    20161 caggcctacg caatctctgc aatatattga atttgcgtgc ttttgtaggc cggataaggc
    20221 gttcactccg catccggcac tctgtgccag taatctgaaa ctcccacgtt gttcaggagt
    20281 tttttatccg cttctggaca ttttctccac agaacaatcg ttagcccgga aggtcgaatc
    20341 tgtgactctt attttcacgg acgaaccaga tgaaagcttc actggcgctg ctcagcctgc
    20401 taacggcatt taccagccac tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa
    20461 tccaggccaa taccaacctt gccatcgcag acggtgccag acagcaaatt ggcagcacgc
    20521 tattttacga tccggcgtat atgcagctta cctatcctgg cggtgatgtt ccgcaagaac
    20581 gcggtgtatg ttccgatgtg gtgatccgcg cattgcgcag ccagaaggtc gatttgcaga
    20641 aactggttca cgaagatatg gcgaagaatt ttgctgagta cccgcaaaaa tggaagttaa
    20701 agcgcccgga cagcaacatc gatcaccgtc gggtgcctaa tctggaaacc tggtttagca
    20761 gccacaacaa aacacgcccc atcagtaaaa atgccggtga ctatcaagcg ggcgatattg
    20821 tctcctggcg attagataac gggctggcgc atattggggt ggcgtcagat ggcttcgccc
    20881 gcgacggtac gccgctggtg atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc
    20941 tgtttagctg gcggatggtg gggcattatc gttattttgt taaataagag atcgccccac
    21001 atgaagaaaa aattcagatg ctttttacac tgacagcttc ttttttctta cggctcacca
    21061 gcgccagtgc cagcacaatc agcacaatac cgctaccttc aacggccccc ggattctcac
    21121 cgagcagcca ccaggaaaag agtacgccac aaacgggaac ggccagtgtg cttaagctgg
    21181 caatactggc aggcaagttt ttcagcacaa acagccataa gctccacgct agcgccgtcg
    21241 ccagaatcgc actgtaggcc agcgcccaga acacggtggg ctgccagtca atttcacgtt
    21301 gcggcaccag taaagcgacc acactcatca ccagcgccgc atacagcatc tgccaggatg
    21361 ttaacgataa taaatccacg cgcggatgac gggcgtatag gcgtttagca acaatcgcgc
    21421 tcgcccccca actaacaccg gagaggattg ccagcatggc acttttcatc gaagagaaat
    21481 cgagttgcca cggctgcaac accaaaaata agccgaaagc ggcaatcaga atcgcgaaat
    21541 attgcccacg tcgcaggcgt tcaccgagaa acaacgcggc gaaaatcacc acccagaacg
    21601 gcatggtata gctcaggatc gccaccttcc ccgcacctcc gctgaccaac gcccactgcg
    21661 ccagaccaac catcccgcag gtttgtaaca gggcaatggc taaggtgtat ttaaacggcg
    21721 tcgggcgcat tccgcgacca cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa
    21781 tgcagcgtaa ggcggtaaag tcgaaggcac cgatgtaact ggtgacttgc ttcatgaaaa
    21841 tccagctata actccagata agtgtgagca caaccaagcc actgattgcc agtgggttgc
    21901 tctttcctgc ggcagacatg agagatccag tgtgtagttt tcctgtggac actatacgcc
    21961 ggtaaagaaa cttcagcgac tgcctgaaag acagtcgctg aagaaggtca gattacagca
    22021 ggtcgaaacg gtcgaggttc atcactttca cccatgccgc tacgaagtct ttaacaaact
    22081 tctcgtgggc atcgctgctg gcgtaaactt ccgctactgc acgcaggaca gagttagaac
    22141 caaataccag atccgcacgg ctggctgtgt atttcacttc gccggtttcg cggtcacgtc
    22201 cttcgaacag ctctttcgat tcgtcagtcg ctttccactc gtaacgcata tccagcaagt
    22261 tcacgaagaa gtcattgctc aatacgccaa cgcggtcagt gaagacgccg tttttgctgc
    22321 catcgaagtt ggcacccagt acacgcatcc cgcccaccag cgcagtcatt tccggcgcgg
    22381 tcagcgtcag ttgctgtgct ttgtcgatca gcagtgactc ggtggtggaa acgtccagac
    22441 gagcgcgata gttacggaaa ccgtcagcaa ttggctccag cagctcaaac atctcaatgt
    22501 cagtctgatc ctgacgcgca tcaacgcgac ccggcgcaaa cggtacatga atgctcaaac
    22561 ctgcggcgct tgcggctttc tcaacaccaa ccacaccagc cagcacgatg atatccgcca
    22621 gcgaggcttt accagactct ttctggattt tctccagaac aggcagagca cgaacggctg
    22681 cggcgttcac atcccagtcg cgctgcggca ttaatgccag acgcgcaccg ttggcaccac
    22741 cgcgtttgtc gccaccacgg aaggtagaag cagatgccca ggccaccgat accagctcac
    22801 taacagacag accagaatcc gcaatcgcga atttcagatc gataatgtcc tgctcggtcg
    22861 ggttgtagat cggctgcggc agcggatctt gccagatcag atcttctttc ggcacttccg
    22921 gcccgatgta gcgagatttc ggccccatat ccctgtgcgt cagtttgaac caggcacggg
    22981 caaaggcttc gttgaacgcc tgcggatcgt tgaggaaacg acgagagatc ttctcgaact
    23041 caggatcaaa acgcagcgtc aggtcggtca ccagcattgt cggtttacgt ttcttcgacg
    23101 gatcgaacgg atccgggata atttccggtg cgtctaccgc ttcgaactgg attgctccag
    23161 ccggactgcg ggtctgtacc cactcatact tgaacagatt ctcgaagaaa tagttgctcc
    23221 actgggtcgg cgtctgggtc cagactactt ccagaccaga ggtaatggca tctgcgccaa
    23281 cgccgctgcc gtaagtgctc gcccagccta aaccttgttc ttcaatcggt gcagcttctg
    23341 gatcaggacc tacgtttgat gtcggaccgg caccgtgggt tttacccagc gtatgaccac
    23401 ccgcaatcag cgccacggtt tcttcgtcgt tcatgcccat gttgccgaag gtcgcgcgga
    23461 tagctgctgc cgcagaaggc ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa
    23521 tcaggcccat ctcggttgca cccagcggtg ctttcgccag cgcttccgga tgacggtgag
    23581 tcagccaggc tttttcatca ccccagttaa catccagatc cggttcccag acgtcttcac
    23641 gaccggcacc aaaaccgaag gtacggaagc cggagttttc cagcgccacg ttacctgcga
    23701 ggataaacag gtcggcccag gagattttct gaccatattt ctgtttgatt ggccacaaca
    23761 ggcgacgcgc tttatcgagg cttacgttat ccggccagga gttcagcggt gcaaaacgtt
    23821 gctgaccacg acccgcgccg ccgcgtccat cgattgaacg gtaagtacct gcgccgtgcc
    23881 aagccatacg aataaacaga ccggcgtagc tgccccagtc ggctggccac cacggctgag
    23941 attctgtcag cagggctttc agatcttttt tcaggccgta gtaatctaat ttgctgaatt
    24001 ctttgcggta gtcaaagtcc tcacccagcg ggttagaacg attagaatgt tggtttaaca
    24061 ggtcaacacg gagttgattt ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct
    24121 ggtcgtgacc gccctgatgg aacgggcatt tgccagtggc tgtggtgtta tggatatcgt
    24181 ctgacgtgct catcaatgtg ctcccctcta cagtgttacc gttacgatac acagcgttag
    24241 agagaagtta taattgaatt aatccacgga tgcgatagtt gagatcttac atattgttgg
    24301 ttaaagagat gtagatcaaa ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg
    24361 acaaatgctt tataaaaaag ggttttagct tcatatcctt caagtaatga gggagaaaac
    24421 aaggctgaca accttatttt cccgccctca tttcgaggca gcattttgtg ctctgtttaa
    24481 aatttgtgat cactgtgtga ttttcacaaa agccacacta tttataaacc aggtcgaaca
    24541 cccagcgtat ggcaaatcgc gtaactcatt tcagcgcggt taagcgtata gaagtggaaa
    24601 tctttcactc cttcacggct taaaatcttc accatatcca tggcaatatt cgcgcccacc
    24661 agtttgcggg tttcggcatc gtcatccagc ccgtcaaaca tttgcgccat ccacgccgga
    24721 atacgcacgt tggtcatgtc ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa
    24781 atgcccggga tgatttccac atcaatgccc gccgatacac agcggtcacg aaaacgcagg
    24841 tagctttcga catcgaagaa gaactgagta atcgcgcggt tggctccggc atccactttg
    24901 cgtttcagat taagcaaatc cgcctgagcg ctttttgctt ccgggtgaac ttccggatac
    24961 gccgccacgg atatatcgaa atctgccact tcttttaaca gcgtcaccag gtcagaagca
    25021 tacatttctg gcttaccact tcccggcggc agatcgccac gcagtgccac gatatgacga
    25081 ataccgttat tccagtagtc gcgtgcaatg gtgcgcagct cgtcgggcgt cgcatcaatg
    25141 caagtaagat gcggtgccgc ttccagacca gtgcgatctt taatgccttt aataatgctg
    25201 tgcgtacggt cgcgctcgcc ggagttcgcg ccataggtca ccgatacaaa cttcggtttc
    25261 aggctgctaa ggcgatcgat ggagttccac agggtctgct ccatttcact ggtacgcggc
    25321 gggaaaaact cgaacgaaac attaatctgc ccctggactt ctgccaggct ctgattcagg
    25381 gcatcccgct ggctggcgtg aaaaaagctc ataccttacc tcatcaatcg cttgtagtta
    25441 tatgttgtgt tgtgcacatc tatccattta gacgtccaga tgtaaagatg aaggaaaagc
    25501 cgaagggcgt caaccgaaaa atgaccaatg atggtgagga aaaatcaggg aagatgaaaa
    25561 aacttcagga tggtaagaaa aagaaaatcc gatatgcaaa gcaaaccgga ctgcaaatat
    25621 aaactaacca tgtacttatt gcttcttgcc ggattcgtcg taaacgcttc atccggccta
    25681 attctatgat aattcaatat atttcattag aaaccataga cccgaaaaca tgagtaccgg
    25741 gcattattaa atttctgaaa ttacaacaac tgtgccagcc ggttgatatc cgactgaatc
    25801 gccccggcgg tgacgtcgcg cccagcgcca ggcccacgga tcaccagcgg attatcgcgg
    25861 taccagcggc tttcgatggc aaagacgtta tcgcatggca gcagtgatgc cagcggatag
    25921 tcttcacgca ccgcctccac gcccacacgc gctttgccgt tggcatcgaa acgcgccacg
    25981 tagcgcagca ccagccccat ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta
    26041 agttcatcgc cattttcaaa gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc
    26101 agcgattcca cacgtacctg gtccggttca atgttgtaac ccgcttcacg cgccagaatc
    26161 accagcttgc gcattacgtc tttgccggag aggtcgtcac gcggatcagg ctcggttaag
    26221 ccctgctgcc acgcctgatc caccagctcg gtaaacggca cgctaccgtc gaattgcagg
    26281 aacagccagg agagcgtgcc ggagaagatc ccactgatcg acaaaatggt atcgccgctg
    26341 tcgatcagat cgcgcacggt atggttgatc ggcaagcccg caccaacggt ggcgttgtat
    26401 agccagtgac gcccggtttt ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg
    26461 cttgctcccg ccagtttgtt ggcactgatg acgtggaaac cgtggctggc gaaatcgaga
    26521 tactgatcag caagctgctg gctggcggta acgtccaaca ccactaaatc atcatacgga
    26581 tgggcgcgca tccacaggaa aagcgactct tcatcctgct cgaccgcttc atcgttgaag
    26641 aaggctaacg cgcggctggc gtccagcccg tcataactca acagactgcg gcggctgtcc
    26701 accacgcctg ccagcacaaa ttcaaagccg gtacgcgccg aaagcgtgct ctgctcgcgg
    26761 gcgaacagtt ccagccagcg agagccgata ttgcctttgc cgaacaatac caggccgatg
    26821 cgtttttctg cgcggaagac ggactgatgc agcccctgaa tcaggctttc ggtcgggccg
    26881 gtgcgcagta ctgccaccag actgatgccg tcatcggact gccacgtaaa ctcgaccggc
    26941 tggcctttca gttgctgcca gaagcggtgg caatgcagcg ggttacgggt gacacccgcc
    27001 ccgaccatcg ccaccagcgc cagcccctga cgcaggcgca gttctccagg taatcccgct
    27061 tcgtcgagga ttttcagcgc actgtcggcc acttctgagg tgtagcaaaa ttgcaacaac
    27121 tggcgatcgt tatgcacgcc aaccgccagt ggacgcacct gggcacgttt caggatctgg
    27181 tcgatctctt tatgcgccag tttgaaatcc tgactggcgg gcacctgaaa ctcaatcaaa
    27241 cagacatcat cgtggctggt gacaatacgc gcaccagtac cggaggccag cacgcgttca
    27301 atgcgcgtgg aaccttgatc cggcgtgtag ctacagcgca gctgcaggtc gatttcgctg
    27361 ccagaaactg gctgtaaagt acgggcgtga agaacgggag ccgccagacg cgccagttcg
    27421 ctggcctcat ccagacgcag caacggcagc aggcaggcat ctttcacttt acgcgggtcg
    27481 gcactgtata ccccggcgac gtcgctccag atggttacac gagaaacacc cgccagcgca
    27541 ccgatttgtg tcgcggaata gtcggaaccg ttacgcccca gcaacaccgt ttcaccggcg
    27601 ttgttacggc tgataaaccc ggtcaccacc agacgtttgc tcggatgttg taccagtagc
    27661 tgttgcagca acgggtaaga aagcccttca tcaacctgcg gttgcgcagc gcgttcagcg
    27721 cgtaaaaact cacgggcatc aagccaggcc gctggcagcc cttgttgatt aagtaccgca
    27781 gacatcaggc gcgccgacca cacttcaccg tggcccacca cttccgcata caccgcatcg
    27841 ttaataccac tgtcgagcaa cgccgccagg cgctcaagat cgctgacaaa agcgctaatg
    27901 aggctatcgg cttcttcagc gggtagcaga ccgctaatca gatcgcactg atagcgacgc
    27961 agcgtttgtt gaacctgatg cgcagagaga cgatcggtct ggcttagttt caaccagtta
    28021 atcaactggt tagtggtgct accggcggcg gaaaccacca tcatatcgtc aggctgagag
    28081 aactccgcca taatgcccgc gacacgcaaa taacacttca catcagccag actactgcca
    28141 ccaaatttat gcagctgacg acctttcgcc cctgcctgcg caatcacact catttttacc
    28201 ccttgtttgc agcccggaag ccattttcca ggtcggcaat taaatcttcg ccatcttcaa
    28261 taccggtgga gatacgcagc agcgtctcgg agatcccggc ggcagcacgc gcttctggtg
    28321 ccatgcctgc atgtgtcatg gttgcggcgt gagagattaa actttccact ccccctaatg
    28381 attccgccag cgtaaacaac gacagcccgc ccaggaaacg acacagcgtc tgctcatcgc
    28441 catccagttc aaaactcaac attgcgccaa agcctttttg ctgacgcgcg gcaatttcat
    28501 gcccctgatt ttccggcagc gacgggtgat acagtttttt caccaacggc tgggtttgca
    28561 gatatttcac aatcgcctgc gcgttgcgct gcgccagctc catacgcggc accagcgttc
    28621 gcaacccgcg tagcagcaga tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg
    28681 cccaccaggc aagttcagtg acaacgtccg ggtctttagc aatcaccacg ccagccacca
    28741 cgtcagagtg accgttcaga tatttcgtac atgaatgcaa caccagatcg gcacccaatg
    28801 ccagcggatt ttgtaatgcc gggcttaaga aggtgttatc caccacgctc accgccccga
    28861 cttcccttgc cagatggcag attttcgcaa tatccacgac gcgtaacaat ggattacttg
    28921 ggctttctac cagtaccagt ttgggttttt ctgccagcgc tgcccgtaat gcctgttcat
    28981 cgccttgatc aacaaacaac acgcgatagc aaccgcgttt cgccagactg tcgaacaggc
    29041 gatagctacc gccgtagcag tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga
    29101 cggtcgttac caggtgaatc gcggacatgc cggtattagt aagtactgcg ccagcaccgc
    29161 cttccagttc tgccagcgca cgctgaacca catcgcgcgt tgggttgccg cgacgcgagt
    29221 aatcatgcgc gcgcggttca ttaaatccgg taaagttata ggtgctggaa agatggatgg
    29281 gagggacaac gcaaccatac tgttcgtcgt catttaaccc gctacgcact gcgatggtgg
    29341 cctgtttacg cgtcatgtga tgaagtttcc tgggctttgt cggtgaaatg tcaggcacca
    29401 gagtaaacat tgtgttaatg gacgtcaata catctggaca tctaaacttc tttgcgtata
    29461 gattgagcaa atcccaaata gccgttaaaa ttatatgcat tatcacgccg acaggtgcat
    29521 tacacgatgt cacggtaacg cctgtacggt aaactatgcg ggtttacggt cagtacccac
    29581 ataaattgtg gggtctggtc tcaatttatt gacgaagagg attaagtatc tcatggctga
    29641 atggagcggc gaatatatca gcccatacgc tgagcacggc aagaagagtg aacaagtcaa
    29701 aaagattacg gtttccattc ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg
    29761 tcgtcaggtg aacaacctgc gtcacgctac caacagcgag ctgctgtgcg aagcgtttct
    29821 gcatgccttt accgggcaac ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga
    29881 cgaaatcccg gaagcggcaa aagagatcat gcgtgagatg gggattaacc cggagacgtg
    29941 ggaatactaa agcggaaaag agcggcgcgg agtggaatcg cctgatgcac tacgcttatc
    30001 aggcctacgt catattgcaa tttattgaat ttgcacgaac ttgtaggccg gataaggcgt
    30061 tcacgccgca tccggcataa acaacgagca cgttgtctgc gacccaccgc tttttataca
    30121 tggacgttta actatgaaaa acaggctgct gatcctcagc ctgctggttt ctgtacctgc
    30181 ctttgcctgg cagccgcaaa ctggcgacat catctttcaa atctctcgct catcgcaaag
    30241 taaagcgatc caactggcaa cccattccga ttacagccac accggtatgc tggtgatgcg
    30301 taacaaaaag ccctacgttt ttgaagcggt cggcccggtg aaatacaccc cgctcaagca
    30361 gtggattgcc catggtgaaa agggcaacta cgttgttcgc cgcgttgaag gcggactgag
    30421 tgttgaacaa cagcaaaaac tgacgcaaac ggcaaaacgt tatctcggta aaccgtatga
    30481 tttcagcttc tcatggagcg acgatcgcca gtactgttcg gaagtggtgt ggaaggttta
    30541 ccagaacgcg ctgggaatgc gcgtgggcga acagcagaag ttgaaagagt ttgatctcag
    30601 caacccgctg gtccaggcga agctcaaaga acgctacggt aaaaatattc cgcttgagga
    30661 aacggtagtt tcaccacaag ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga
    30721 gtggccgctg ttttcgtggt gatacgacca aaattcaggc acaaaaaaag cgccgtgcgg
    30781 cgcttttttc ggaaatccgg tcttatttgc tgcccgggat gttgaaacgc ttgttgaagc
    30841 ggtcaacacg gccaccggta gcaacatcac gctgtttgcc agtgaagaac gggtggcact
    30901 tgctgcacac gtcgaggttc aggtcatgac caacggtgga gcggattttt attacgttac
    30961 cgcaagagca gctagcagta atttcttcgt atttcgggtg aatatctttt ttcatgggaa
    31021 aacctcggtt taaggccgcg tcgctcttcc agccctaacg ccagacacca cgcgatgtta
    31081 aaagtatagc ttcaatacga tcatttcgta cgaaggcgcg aaatcataca gaaattaacc
    31141 agcgtatgca aactgatccg cactcttcta cggcaatgtg tatactaacc caccgaattt
    31201 caagtcagga tgatgctatg cccgttgccc acgttgcctt gcccgttccg cttcctcgta
    31261 cctttgacta tctgctgcca gaaggcatga cggttaaagc tgggtgtcgc gtgcgcgtgc
    31321 cgtttggcaa acagcaggag cgcatcggga ttgtggtatc agttagcgat gccagcgaac
    31381 tgccgctcac tgagctaaaa gcggtagtcg aagtgctgga tggtgagccg gtgtttactc
    31441 actccgtctg gcgattgctg ctatgggcgg cagattacta tcatcatccg attggcgatg
    31501 tgctgtttca tgccttgccg attttactac gccaggggcg gcctgcggcg aacgcgccga
    31561 tgtggtactg gtttgccact gaacaaggtc aggcggtgga tctgaacagc ctgaaacgct
    31621 cacccaagca acaacaggcg ctggcagcgt tacggcaagg caaaatctgg cgcgaccagg
    31681 ttgccacgct cgaatttaat gatgccgcgt tgcaggcgct gcgcaaaaaa ggtctgtgtg
    31741 atttagcaag tgaaacacca gagtttagcg actggcgaac gaactatgcc gtttctggtg
    31801 agcggttgcg attgaatacc gaacaggcca ccgccgttgg cgcaattcat agcgcggcag
    31861 atactttttc tgcctggctg ctggcgggcg ttaccggttc cggtaaaacg gaggtttatc
    31921 tcagtgtact ggaaaacgtg ctcgctcagg gcaaacaggc gctggtgatg gtgccggaaa
    31981 tcggcctgac accgcaaact atcgcccgtt ttcgtgaacg gtttaatgcg cccgtggaag
    32041 ttctgcattc cggtctgaac gacagcgagc gtctttcggc gtggctgaaa gcgaaaaatg
    32101 gcgaggcggc gattgtgatc ggcacccgct ccgcgctgtt tacgccgttt aaaaatctcg
    32161 gcgtgattgt cattgacgaa gagcacgaca gttcctacaa gcagcaggaa ggctggcgct
    32221 atcatgcccg cgacctggcg gtgtatcgtg cgcatagcga gcaaatcccg attattcttg
    32281 gctccgcaac gcccgcgctg gaaacgttgt gcaacgtcca gcagaaaaaa taccgcctgc
    32341 tgcgcctgac gcgccgggcg gggaatgcgc gtccggcaat tcaacacgtg ctggatttaa
    32401 aaggtcagaa ggtgcaggca ggtctggctc cggcgttaat cacccgtatg cgccagcatt
    32461 tacaggccaa caatcaggtt atcctctttc ttaaccgccg tggctttgcg cctgcgctac
    32521 tgtgccacga ctgcggctgg attgccgaat gcccacgttg cgatcactac tacacgctgc
    32581 atcaggcgca acagcatttg cgctgtcacc actgtgacag tcagcgtccg gtgccgcgcc
    32641 agtgcccttc ctgcggttcc acgcacctgg tacccgtggg gctgggcacc gaacagcttg
    32701 aacagacgct tgcgccgctg ttccctgatg tgcccatttc tcgtatcgac cgcgatacca
    32761 ccagccgcaa aggggcgctg gaacagcaac tggcggaagt acatcgcggc ggtgcgagga
    32821 ttttgatcgg cacacaaatg ctggcgaaag gtcaccactt tccggatgtg acgctggtgg
    32881 cgttactgga tgtggacggc gcgctgtttt ctgccgattt tcgctcggca gagcgtttcg
    32941 ctcagcttta cacccaggtc gctggtcgtg ccgggcgtgc gggtaaacaa ggcgaagtgg
    33001 tgctgcaaac gcaccatccg gaacatcctc tgttgcaaac gttgctctat aaaggctacg
    33061 acgcctttgc cgaacaggcg ctggctgagc gacggatgat gcaactgccg ccatggacca
    33121 gccatgtgat tgtgcgtgcg gaagatcata acaatcagca tgcgccgctg ttcctgcaac
    33181 aactgcgtaa tctgatcctc tccagcccgc tggcagacga caaactgtgg gttctcggtc
    33241 cggttccggc actggcacct aaacgtggcg gtcgctggcg ctggcagata ttgttgcagc
    33301 acccttcccg cgtgcgcttg caacacatca ttagtggtac gctggcgctc atcaatacaa
    33361 taccggattc ccgcaaggtg aaatgggtac tggatgttga tccgattgag ggttaaaccg
    33421 cttacgatgc gaggcggatc gaaaaattca atattcatca cacttttcat gaaaattctg
    33481 taaccgtttt cacgcgctat ctgctaaaaa tgttgccgat gtgaagtaaa catggatgta
    33541 gtacgcctga cgtgccaggc gaggagtgag tgtgaaagcg aagaagcagg aaactgccgc
    33601 gaccatgaaa gacgttgccc tcaaggcaaa agtctctaca gcgactgtct cccgagcatt
    33661 aatgaatccc gataaagtct cccaggccac ccgtaatcgg gttgaaaaag cggcccggga
    33721 agtgggttat ttaccgcagc ctatggggcg caacgtcaag cgtaatgaat cccgcaccat
    33781 tctggtgatt gtcccggata tctgcgatcc cttctttagc gaaattattc gcggtatcga
    33841 agttacggcg gcaaatcacg gatatctggt gctgattggc gactgtgcgc atcaaaatca
    33901 gcaggaaaaa acctttatcg atttgatcat caccaagcaa attgatggca tgttgctgct
    33961 gggttcaagg ctgccgtttg atgccagcat tgaggaacag cgtaatctgc cgccgatggt
    34021 gatggcgaac gaatttgcac cggaactgga gctgcctacc gttcatatcg acaatctgac
    34081 cgccgcattt gatgcagtaa attatttata tgagcaaggg cataaacgga ttggctgtat
    34141 agccggtccc gaagagatgc cactgtgtca ctaccgcctg caaggctatg ttcaggcgct
    34201 gcgtcgctgc ggaattatgg ttgatccaca atacatcgcc cgtggcgact tcaccttcga
    34261 agccggaagc aaagcaatgc aacagctgct tgatcttcca caaccgccta ctgcagtctt
    34321 ctgccatagc gatgtgatgg cgctcggcgc actttctcag gcaaaacgcc aggggctgaa
    34381 agtcccggaa gacctttcca taatcggttt tgataacatc gacctgacgc aattttgtga
    34441 tccgccgctg acaaccatcg cgcagccgcg ttacgaaatc ggtcgggaag ctatgctgtt
    34501 attgcttgat caaatgcagg ggcaacacgt tggcagtggc tctcgtttaa tggactgcga
    34561 acttatcatc cggggatcaa cacgcgcgtt accttaaagt aaacctttaa gactgccgtg
    34621 actggtcaaa ggcccgccgc ttaagtaaca tggcgggctg acgaacgaat aaatacagcg
    34681 aaacgatagt ggcacaacga gattatgtac gccgcagcca accggcacct tcgcggcgaa
    34741 aaaagagcac ctcacggaaa aagcaacgaa atctgcctgc ggtttctccc gctatggtcg
    34801 ctattgctgc cgccgttctt gtgaccttta tcggtggtct gtacttcatt acgcatcaca
    34861 agaaagaaga gtccgagacg ctgcaaagcc agaaagtgac cggaaacgga ctaccaccaa
    34921 aaccagaaga acgctggcgc tacattaaag agctggaaag tcgccagccg ggtgtgcgtg
    34981 cgcccacaga accttctgcc ggtggtgaag tgaaaacgcc ggagcaactg acaccagaac
    35041 aacgtcagct tcttgaacaa atgcaggctg atatgcgcca gcagccaacg cagctggttg
    35101 aggttccgtg gaatgaacag acgccggaac agcgtcagca aacgctacaa cgccagcgtc
    35161 aggcgcagca attagcggag cagcaacgcc tggctcagca gtcgcgtacg actgaacaaa
    35221 gctggcagca gcagacgcgt acgtcgcaag ccgcgccggt acaggcgcaa ccgcgccagt
    35281 ctaaaccagc ttctactcag cagccgtacc aggatctgct gcaaacccct gcgcacacga
    35341 ccgcgcagtc gaagccgcag caagctgcgc cggttgctcg tgccgctgac gcgccaaaac
    35401 cgacggcgga gaaaaaagac gaacgccgct ggatggtgca gtgcggttcg ttcagaggcg
    35461 cggaacaggc agagacggta cgtgctcagc tggcgttcga aggatttgac tcgaaaatca
    35521 ccaccaacaa tggctggaat cgtgtggtca ttggcccggt gaaaggcaaa gagaacgcag
    35581 acagcaccct caatcggttg aagatggcgg gtcatacaaa ctgcattcgg ctcgccgccg
    35641 ggggttgaaa ccctcaaaat cccccccatc tataattgca ttatgccccg tacttttgta
    35701 cggggtttgt actctgtatt cgtaaccaag gggtcagctc gtgacaacta tagtaagcgt
    35761 acgccgtaac ggccatgtgg tcatcgctgg tgatggtcag gccacgttgg gcaataccgt
    35821 aatgaaaggc aacgtgaaaa aggtccgccg tctgtacaac gacaaagtca tcgcgggctt
    35881 tgcgggcggt actgcggatg cttttacgct gttcgaactg tttgaacgta aactggaaat
    35941 gcatcagggc catctggtta aagccgccgt tgagctggca aaagactggc gtaccgatcg
    36001 catgctgcgc aaacttgaag cactgctggc agtcgcggat gaaactgcat cgcttatcat
    36061 caccggtaac ggtgacgtgg tgcagccaga aaacgatctt attgctatcg gctccggcgg
    36121 cccttacgcc caggctgcgg cgcgcgcgct gttagaaaac actgaactta gcgcccgtga
    36181 aattgctgaa aaggcgttgg atattgcagg cgacatttgc atctatacca accatttcca
    36241 caccatcgaa gaattaagct acaaagcgta aggatctccc atgtctgaaa tgaccccacg
    36301 cgaaatcgtc agcgaactgg ataagcacat catcggccag gacaacgcca agcgttctgt
    36361 ggcgattgct ctgcgtaacc gctggcgtcg catgcagctc aacgaagagc tgcgccatga
    36421 agtgaccccg aaaaatatcc tgatgatcgg cccgaccggt gtcggtaaaa ctgaaatcgc
    36481 ccgtcgtctg gctaagctgg cgaatgcgcc gttcatcaaa gttgaagcga ccaaatttac
    36541 cgaagtgggc tacgtcggta aggaagttga ttctattatt cgcgatctga ccgatgccgc
    36601 cgtgaaaatg gtacgcgtcc aggctatcga gaaaaaccgt tatcgcgctg aagaactggc
    36661 agaagaacgt attctcgacg tgctgatccc acctgctaaa aacaactggg gacagactga
    36721 acagcagcag gaaccttccg ctgctcgtca ggcattccgc aaaaaactgc gtgaaggcca
    36781 gcttgatgac aaagaaatcg agatcgatct tgccgcagca ccgatgggcg ttgaaattat
    36841 ggctcctccg ggcatggaag agatgaccag ccagctgcag tccatgttcc agaacctggg
    36901 cggccagaag caaaaagcgc gtaagctgaa aatcaaagac gccatgaagc tgctgattga
    36961 agaagaagcg gcgaaactgg tgaacccgga agagctgaag caagacgcta tcgacgctgt
    37021 tgagcagcac gggatcgtgt ttatcgacga aatcgacaaa atctgtaagc gcggcgagtc
    37081 ttccggtccg gatgtttctc gtgaaggcgt tcagcgtgac ctgctgccgc tggtagaagg
    37141 ttgcaccgtt tccaccaaac acgggatggt caaaactgac cacattctgt ttatcgcttc
    37201 tggcgcgttc cagattgcga aaccgtctga cctgatcccg gaactgcaag gtcggctgcc
    37261 aatccgcgtt gaactgcagg cactgaccac cagcgacttc gagcgtattc tgaccgagcc
    37321 gaatgcctct atcaccgtgc agtacaaagc actgatggcg actgaaggcg taaatatcga
    37381 gtttaccgac tccggtatta aacgcatcgc ggaagcggca tggcaggtga acgaatctac
    37441 cgaaaacatc ggtgctcgtc gtttacacac tgttctggag cgtttaatgg aagagatttc
    37501 ctacgacgcc agcgatttaa gcggtcaaaa tatcactatt gacgcagatt atgtgagcaa
    37561 acatctggat gcgttggtgg cagatgaaga tctgagccgt tttatcctat aatcgcgttc
    37621 aatcattttc atcattgttt gatggggctg aaaggcccca tttttattgg cgcgtattat
    37681 gactgaacaa caaattagcc gaactcaggc gtggctggaa agtttacgac ctaaaaccct
    37741 ccccctcgcc tttgctgcaa ttatcgtcgg gacggcgctg gcatggtggc aaggtcactt
    37801 cgatccgctg gtcgccctgc tggcactaat taccgccggg ctattacaga tcctttctaa
    37861 cctcgccaat gattacggcg atgcggtaaa aggcagcgat aaacctgacc gcattgggcc
    37921 gctacgcggc atgcaaaaag gggtcattac ccagcaaaag atgaaacggg cgctcattat
    37981 taccgtcgtg ctcatctgtc tctccgggct ggcactggtt gcagtggcat gccatacgct
    38041 ggccgatttt gtcggtttcc tgattcttgg cgggttgtcg atcattgccg ctatcaccta
    38101 caccgtgggc aatcgtcctt atggttatat cggtctgggt gatatttccg tactggtttt
    38161 ctttggctgg ttgagtgtca tggggagctg gtatttacag gctcatacat tgattccggc
    38221 actgatcctt ccggcgaccg catgcggcct gctggcaacg gcagtactga atattaataa
    38281 cctgcgtgat atcaatagcg accgcgaaaa tggcaaaaac acgctggtgg tgcgcttagg
    38341 tgaagtgaac gcgcgtcgtt atcatgcctg cctgctgatg ggctcgctgg tgtgtctggc
    38401 gctgtttaat ctcttttcgc tgcatagcct gtggggctgg ctgttcctgc tggcggcacc
    38461 attactggtg aagcaagccc gttatgtgat gcgggaaatg gacccggtgg cgatgcgacc
    38521 aatgctggaa cgtactgtca agggagcgtt actgactaac ctgctgtttg ttttagggat
    38581 attcctaagc cagtgggcag cataactgac aaatatcaat taacaattga tgattttgcc
    38641 aacagcccac atagcgcgat atactgaaaa ttctcgcagc aactgaatgt taagcctatg
    38701 aaatacgata cttccgagct ttgtgacatc tatcaagaag atgttaacgt cgtggaaccg
    38761 ctgttctcca actttggcgg acgggcgtcg tttggcggac aaataatcac ggtaaaatgt
    38821 ttcgaggaca acgggttgct gtacgatctg ctcgaacaga atggccgtgg tcgtgttctt
    38881 gtcgtcgatg gcggtggttc tgttcgtcgc gcactggtcg atgctgaact ggcgcgtctg
    38941 gcagtacaaa atgaatggga aggtctggtc atttacggcg cggtgcgtca ggtagatgac
    39001 ctggaagagt tggatatcgg catccaggcg atggcggcaa ttccggttgg tgccgctggc
    39061 gaaggcattg gcgaaagcga tgtccgcgtc aattttggcg gtgtcacctt cttctccggc
    39121 gaccatcttt atgccgacaa taccgggatt attctttcag aagatccgct ggatattgaa
    39181 tgataagaaa ggcaccgcaa ggtgcctttt ttctgcgtta cctgttggcc tacacagtaa
    39241 agaaattacg cgaaagatga agcgtaatca gacctcttcc atgcgaccca gcagggcctg
    39301 cagacgttcc tgccagccgt tctgctgttc tttcagatgg ttgttctcac gctccagctc
    39361 ttcgcgctga tgctgggcat tttgaacttc ctgcgacagt gagttgtttt tttctttcag
    39421 ctcttcgatt tccatctgca acagagtgat ggtatcaatc gcctgctgta cttttgcttc
    39481 cagtttctca aacacttcta atgacattgt catacctctc ctgaattgca aggcgttgat
    39541 ggataaaaaa tcctcgtccc gattaccggt gacgccttaa taaatacgag cgcactttag
    39601 ttagctccga ttgtatgaag ccgcgccatc gctgtccagc ggcacgcctt gcagattacg
    39661 gtttgccaca cttttcatcc ttctcctagt gacataatcc acatcaatcg aaaatgttaa
    39721 taaatttgtt gcgcgaatga tctaacaaac atgcatcatg tacaatcaga tggaataaat
    39781 ggcgcgataa cgctcatttt atgacgaagc acacacattt taagttcgat atttctcgtt
    39841 tttgctcgtt aacgataagt taacagcatg cctacaagca tcgtggaggt ccgtgacttt
    39901 cacgcataca acaaacatta actcttcagg atccgattat gagtcaaaca tcaaccttga
    39961 aaggccagtg cattgctgaa ttcctcggta ccgggttgtt gatttttttc ggtgtgggtt
    40021 gcgttgcagc actaaaagtc gctggtgcgt cttttggtca gtgggaaatc agtgtcattt
    40081 ggggactggg ggtggcaatg gccatctacc tgaccgcagg ggtttccggc gcgcatctta
    40141 atcccgctgt taccattgca ttgtggctgt ttgcctgttt cgacaagcgc aaagttattc
    40201 cttttatcgt ttcacaagtt gccggcgctt tctgcgctgc ggctttagtt tacgggcttt
    40261 actacaattt atttttcgac ttcgagcaga ctcatcacat tgttcgcggc agcgttgaaa
    40321 gtgttgatct ggctggcact ttctctactt accctaatcc tcatatcaat tttgtgcagg
    40381 ctttcgcagt tgagatggtg attaccgcta ttctgatggg gctgatcctg gcgttaacgg
    40441 acgatggcaa cggtgtacca cgcggccctt tggctccctt gctgattggt ctactgattg
    40501 cggtcattag cgcatctatg ggcccattga caggttttgc catgaaccca gcgcgtgact
    40561 tcggtccgaa agtctttgcc tagctggcgg gctggggcaa tgtcgccttt accggcggca
    40621 gagacattcc ttacttcctg gtgccgcttt tcagccctat cgttggcgcg attgtaggtg
    40681 catttgccta ccgcaaactg attggtcgcc atttgccttg cgatatctgt gttgtggaag
    40741 aaaaggaaac cacaactcct tcagaacaaa aagcttcgct gtaatatgac tacgggacaa
    40801 ttaaacatga ctgaaaaaaa tatatcgttg cgctcgacca gggcaccacc agctcccgcg
    40861 cggtcgtaat ggatcacgat gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa
    40921 tctacccaaa accaggttgg gtagaacacg acccaatgga aatctgggcc acccaaagct
    40981 ccacgctggt agaagtgctg gcgaaagccg atatcagttc cgatcaaatt gcagctatcg
    41041 gtattacgaa ccagcgtgaa accactattg tctgggaaaa agaaaccggc aagcctatct
    41101 ataacgccat tgtctggcag tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg
    41161 acggtttaga agattatatc cgcagcaata ccggtctggt gattgacccg tacttttctg
    41221 gcaccaaagt gaagtggatc ctcgaccatg tggaaggctc tcgcgagcgt gcacgtcgtg
    41281 gtgaattgct gtttggtacg gttgatacgt ggcttatctg gaaaatgact cagggccgtg
    41341 tccatgtgac cgattacacc aacgcctctc gtaccatgtt gttcaacatc cataccctgg
    41401 actgggacga caaaatgctg gaagtgctgg atattccgcg cgagatgctg ccagaagtgc
    41461 gtcgttcttc cgaagtatac ggtcagacta acattggcgg caaaggcggc acgcgtattc
    41521 caatctccgg gatcgccggt gaccagcagg ccgcgctgtt tggtcagttg tgcgtgaaag
    41581 aagggatggc gaagaacacc tatggcactg gctgctttat gctgatgaac actggcgaga
    41641 aagcggtgaa atcagaaaac ggcctgctga ccaccatcgc ctgcggcccg actggcgaag
    41701 tgaactatgc gttggaaggt gcggtgttta tggcaggcgc atccattcag tggctgcgcg
    41761 atgagatgaa gttgattaac gacgcctacg attccgaata tttcgccacc aaagtgcaaa
    41821 acaccaatgg tgtgtatgtg gttccggcat ttaccgggct gggtgcgccg tactgggacc
    41881 cgtatgcgcg cggggcgatt ttcggtctga ctcgtggggt gaacgctaac cacattatac
    41941 gcgcgacgct ggagtctatt gcttatcaga cgcgtgacgt gctggaagcg atgcaggccg
    42001 actctggtat ccgtctgcac gccctgcgcg tggatggtgg cgcagtagca aacaatttcc
    42061 tgatgcagtt ccagtccgat attctcggca cccgcgttga gcgcccggaa gtgcgcgaag
    42121 tcaccgcatt gggtgcggcc tatctcgcag gcctggcggt tggcttctgg cagaacctcg
    42181 acgagctgca agagaaagcg gtgattgagc gcgagttccg tccaggcatc gaaaccactg
    42241 agcgtaatta ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg gcgtgggaag
    42301 aacacgacga gtaatgtcag gcacctgaat cgattgcctg atgctgatcg cctgatgcga
    42361 cgcttacgcg tcttatcagg cctacgagcc aatgccattt tgtgtaggcg ggataaggcg
    42421 ttcacgccgc atccggcatc acgcagactc tgaaaccgtg cgggggcaac cccgcacaca
    42481 tcaataatcc cacccttccc ctgtgctaca cttcgcgcca ttccttactg cttagagttt
    42541 gctatgagac gagaacttgc catcgaattt tcccgcgtca ccgaatctgc ggcgctggct
    42601 ggctacaaat ggttaggacg cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc
    42661 atgcgtatta tgctcaacca ggtcaacatt gacggcacca tcgtcattgg tgaaggtgaa
    42721 atcgacgaag caccgatgct ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg
    42781 gtagatattg ctgttgatcc gattgaaggc acgcgcatga cggcgatggg ccaggctaac
    42841 gcgctggcgg tgctggcagt aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat
    42901 atggagaagc tgattgtcgg gccgggagcc aaaggcacca ttgatctgaa cctgccgctg
    42961 gcggataacc tgcgcaatgt agcggcggcg ctcggcaaac cgttgagcga actgacggta
    43021 acgattctgg ctaaaccacg ccacgatgcc gttatcgctg aaatgcagca actcggcgta
    43081 cgcgtatttg ctattccgga cggcgacgtt gcggcctcaa ttctcacctg tatgccagac
    43141 agcgaagttg acgtgctgta cggtattggt ggcgcgccgg aaggcgtggt ttctgcggcg
    43201 gtgatccgcg cattagatgg cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa
    43261 ggcgacaacg aagagaatcg tcgcattggc gagcaggagc tggcacgctg caaagcgatg
    43321 ggcatcgaag ccggtaaagt attgcgtctg ggcgatatgg cgcgcagcga taacgtcatc
    43381 ttctctgcca ccggtattac caaaggcgat ctgctggaag gtattagccg gaaaggcaat
    43441 atcgcgacta ccgaaacgct gctgatccgc ggcaagtcac gcaccattcg ccgcattcag
    43501 tccatccact atctggatcg caaagacccg gaaatgcagg tgcacattct ctgagtgatt
    43561 tgatcgattg agccttccag tccttcggga ctggaatttt tttgttcgga gaacgaagat
    43621 aaggcaagtc aatcaaaaca ggagaaaaac atggctgatt gggtaacagg caaagtcact
    43681 aaagtgcaga actggaccga cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg
    43741 tttaccgccg ggcaatttac caagcttggc cttgaaatcg acggcgaacg cgtccagcgc
    43801 gcctactcct atgttaactc gcccgataat cccgatctgg agttttacct ggtcaccgtc
    43861 cccgatggca aattaagccc acgattggcg gcactgaaac cgggcgatga agtgcaggtg
    43921 gttagcgaag cagctggctt ctttgttctg gatgaagtac cggactgcga aacgctatgg
    43981 atgctggcaa ccggtacagc gattggccct tatttatcga ttctgcaact aggcaaagat
    44041 ttagatcgct tcaaaaatct ggtcctggtt cacgccgcac gttatgccgc cgacttaagc
    44101 tatttgccac tgatgcaaga actggaaaaa cgctacgaag gcaaactgcg cattcagacg
    44161 gtggtcagtc gggaaacggc agcggggtcg ctcaccggac ggataccggc attaattgaa
    44221 agtggggaac tggaaagcgc gattggcctg ccgatgaata aagaaaccag ccatgtgatg
    44281 ctgtgcggca atccacagat ggtgcgcgat acacaacagt tgctgaaaga gacccggcag
    44341 atgacgaaac atttacgtcg ccgaccgggc catatgacag cggagcatta ctggtaagcg
    44401 gttacttatc gataaacggc acgatgagca aatccgcact catcttattg atcatcccgc
    44461 gatatgccgg catcaaacgg ttgataaatg agtgatgatg accacagaca aggaggtcgc
    44521 actgctcttt ttgcataatt tccagcagtg tttccggcat ttctccgcgt tcaatacgca
    44581 gttttgtctt cggccattga atatttttcg tcagtttata cagcttgtta tccgacttat
    44641 tcttcaacaa ttgaagaata tcttctgttg cagggaagta gatacccgga tacaactcgc
    44701 ttaagccatc atcaatatga attaacgtca ggtgagcgtc attatgtctg gcgagctcca
    44761 gggctttatt caccagtaag gcatcttctt cattcccgga aattgccacg ccaatgtgtt
    44821 tataagccat gtttaactcc ttctaaagca actccatcaa gctaacgaac gcagtgataa
    44881 ctcaaaaata atcatctcgg cctggcatga gaaagtgaag gccgcatcaa gctcaacttg
    44941 cccctccaga tccttaatct tataggccac gctggcccct tcatctgcag aggatgcagc
    45001 ggcggcaagc tctttcagtt tggtcataaa agactctgct tcttcgcggg tggcaaaaaa
    45061 gcgcgaaaac ttactggtac agttgtcgtt atcaatcacc gtaccgatat caatcgcaca
    45121 tcctttagta ctgcatttat ctacgacatc tttcataggg cacctctgta tattcgcctc
    45181 tctgtcgatt acgacttaag aataatgcgc cgttaaggga agaacaaaca agtaatgatc
    45241 ttatggatta ccattttttt tgatattgct taaggtgaag agttcagaag gattttccgg
    45301 gtattgtcat ttgcatcgtg gaaataacaa tcccggaaaa aaatagcagt aagagatata
    45361 ttaaaggtta attacttgat ttattgtcgg ctttatactt cacatcctga gtatctttac
    45421 catatttatt ttcgccttgt gtgccaacaa acgcaccaag atcgataagc atcatcacca
    45481 gaatcaacgt cgggacaaaa cgccccaccg cccattgcca gacacccggt aaaatcgctc
    45541 agttacccgc cagcagcatc cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc
    45601 cgcgatcatg caagcgctta acagttactg ccgctgttgg ccagagcaag cacacaaggc
    45661 aaaacgccgc ggtctgaata tcgagtagat tcttacccgc cagtgagaaa agcaccagca
    45721 tgcctgcgaa ccacaggcct atccaaatcc agaaatcacg gcgtccaata cgccctttaa
    45781 atgagaataa ccattgctgt atggtcatgt aagttccttg atggttgtct tttccaggat
    45841 tctacccttt tgacaagggc gacagatatc gttttaatcg gagccagtca taacaaaagg
    45901 tactgtcaat gaagccaggg tgtacgctgt tttttctctt atgttctgca ttaaccgtta
    45961 caacaacggc gcatgcacaa acaccagata cggcaacgac cgcgccttat ctgctggctg
    46021 gagcccctac tttcgatctc tccatcagcc agtttcgaga agactttaac agccagaatc
    46081 ccagcctgcc actgaacgaa tttcgtgcca tcgacagcag tcccgacaaa gccaatctca
    46141 ctcgtgctgc aagtaaaatt aacgagaact tgtatgcttc tacagcgctg gagcgcggta
    46201 ccttaaaaat caaaagcatt caaatgacct ggctacccat ccaggggcca gagcaaaaag
    46261 cagcgaaagc gaaagctcag gaatacatgg cagcagtgat ccgcacactc accccattaa
    46321 tgaccaaaac acaaagccag aaaaaactgc agtcgctact aacggcgggg aaaaacaaac
    46381 gttattacac cgagacagaa ggtgcactgc gttatgttgt cgcggacaac ggcgaaaagg
    46441 ggctgacctt cgctgttgaa ccgattaagc tggcgctatc tgaatcgctt gaaggtttga
    46501 ataaatgaca aaaagcaaag cctttgtgcc gatgaatctc tatactgttt cacagacctg
    46561 ctgccctgcg gggcggccat cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg
    46621 acatccttta gtgatgggta actggaaact gaacggcagc cgccacatgg ttcacgagct
    46681 ggtttctaac ctgcgtaaag agctggcagg tgttgctggc tgtgcggttg caatcgcacc
    46741 accggaaatg tacatcgata tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg
    46801 tgcgcaaaac gtggacctga acctgtccgg cgcattcacc ggtgaaactt ctgccgctat
    46861 gctgaaagac atcggcgctc agtacatcat catcggccac tctgaacgtc gtacttacca
    46921 caaagagtct gacgaactga ttgcgaaaaa attcgcggtg ctgaaagagc agggtctgac
    46981 tccggttctg tgcatcggtg aaaccgaagc tgaaaacgaa gcgggaaaaa ctgaagaagt
    47041 ttgcgcacgt cagatcgacg cggtactgaa aactcagggt gctgcggcat tcgaaggtgc
    47101 ggttatcgct tacgaacctg tatgggcaat cggtactggc aaatctgcaa ctccggctca
    47161 ggcacaggct gttcacaaat tcatccgtga ccacatcgct aaagttgacg ctaacatcgc
    47221 tgaacaagtg atcattcagt acggcggctc tgtaaacgcg tctaacgctg cagaactgtt
    47281 tgctcagcca gacatcgacg gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt
    47341 cgcagtgatc gttaaagctg cagaagcggc taaacaggct taagtctgac aggtgccgga
    47401 tttcatatcc ggcacttact ttccttaact cctcgcctta acgcaaaatc tcacactcat
    47461 gatcctggat ttcctcggct gaagcacggt tcagcgtcag tagatttcgt tgtgtcgcca
    47521 gcaacacaaa cgaattatca ctctgccgta ccatcgccag cccgtagctt cccatatgtt
    47581 cccgcgcctc aggcacttct tctgcaagca tcataaatgg gctgcgttgt accagttcgc
    47641 tttccgttac ccgacgcgcc aggtattcat gcccgcgcaa accacctggc agtggcaacc
    47701 agcggctgct gatgttcgcc agatttttat ccagttgttc gcgcacatca ggacgaatac
    47761 aagagatatg aatatgaaaa tggttttgcg tacgcccggt gcgggagttg atcgccaaag
    47821 aaaccgcgcg atcgggaacc ggctggccgt attttttgct cataaaatca cgcgcctgcc
    47881 aggccaacca aaagaagttc ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt
    47941 taatacgata cgttggcatc aacagatatt gcagtggacc attaagatct tttaaaacca
    48001 cgtatccggc attgggtttg acttccgcac atggcgaagg attttgattt tgctgctgat
    48061 tgggcaaaca ttcctcaagg acaatcttac gtaatgtatc cgactcttca ccggttaatt
    48121 tccagtaacc aataccggca gccacaacgg cgataactat catcaccaaa aaaagaagac
    48181 ccgctttttt catctttttt tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg
    48241 tgacaaataa aaaacccggt cgcgagaaac caccgggttt gataattatc ctagggaaat
    48301 cagcgtttgc tgatctgatc gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc
    48361 gtccagccgc cgaactcttc atcaatggtg aataacttca gctttggaaa cgcattttcg
    48421 tactttttcg ctacttcagc gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga
    48481 ccttctggcg agtagagata tttcaggtag gcttccgcca cctctttggt gccttttttc
    48541 tcgaccactt tatcgaccac cgacaccgtt ggctctgcga ggatagactc actcggcgtg
    48601 acgatttcga atttatcttt ccccagttca ttcgctgcca gcagagcttc gttttcccag
    48661 gcgatcagta catcgccaat cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca
    48721 gaatccagaa cttcgacgtt tttatacagt gcccgaacaa aatcctgtgc ttttgcctga
    48781 tcgttgttgt tgtgatgcag cgcgtagccc caggcagcca ggtagttcca gcgtgcgcca
    48841 ccagagcttt tcggattagg cgtgatcacc gaaacacccg gtttaatcag atcgttccag
    48901 tcatggatct gcttcggatt gcccttacgc accaggaaaa caatggtgga agtgtacggt
    48961 gcggagttat ccggcagacg tttgatccac tctttatcaa tccgcccgcg ttccgcaatc
    49021 gcgtccacgt cataggccag agccagcgtg acaacatcag cttcaatacc gttgattacc
    49081 gacgtcgctt gtttacctga accaccgtgt gactgacgaa tcaccacgtt atcaccagtt
    49141 tgctgtttcc agtgggcgct gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga
    49201 tcatatgaaa cgttaagaag ctgaatatcc tttgccataa cgctggttgc cgccagcaaa
    49261 aatgttaacc ctacgcccca cttgttcatc gcccgactct cttatgttgt gttgtgatga
    49321 gcaaagcgtg ccagaaggtt aaccaaacat taaagaataa aaaaagattg gctataactt
    49381 gcgggtatat gttgagggat taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag
    49441 tgcgctttgt ccatgccgga tgcggcgcga acgccttatc cggcctacaa aagtttgcaa
    49501 attcaataaa ttgcagaatt catgtaggcc tgataagcga agcgcatcag gcatttttgc
    49561 ttctgtcatc ggtttcaggc taaaggaatc tgcctttttc cgaaatcatt aatacagttt
    49621 tttcgcgcag tccagccagt cacctttgaa cggacgcttc atgttttcga tagcgtcgat
    49681 gatgtcgtgg tgaaccagct gttcgttctg gatacctaca caacgaccgc cgtaacctgc
    49741 cagcagcaga tcgatagcgt aagcgcccat acgggaagcc agaatacggt cgtaaggcac
    49801 cggagaacca ccgcgctgga tgtggcccag cacagttgcg cgggtttcac gaccggtttc
    49861 tttctcgatg aaatgcgcca gttcgtcaac atcacacata tgttcggtaa tcgccacgat
    49921 cgcgtgtttt ttacctttcg cgatacccgc tttgatttcg tttaccaggt cttcacggct
    49981 gaattcaact tccggaacta caacgaattc acagccaccg gcaatggccg cagccagggt
    50041 cagatcgcca caataacggc ccatcacttc caccacggaa atacgctggt gagaagaaga
    50101 ggtgtcacgc agacggtcga tcgcttctac aacggtgctc agcgcagtga agaaaccgat
    50161 agtgtagtca gtgcctttga tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa
    50221 gcccatttcg gtcagacgca ttgcacccat gtaggaaccg tcaccgccga taaccaccag
    50281 cgcgtcgata ccacgttttt tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg
    50341 gaattccggg aaacgcgcag aaccgaggaa cgtaccgcca cggttgatca tgtcagacac
    50401 gctgtaacgg tctagctgta ccatacggtc ttcatacaga cccagatagc cgtcataaat
    50461 acccattact tccagacctt ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt
    50521 catgcctggc gcatcaccgc cgcttgtcaa cacacctatt ttcttaatca tgactacctc
    50581 tgaactttgg aatgcaaaat gaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat
    50641 ccaacgaatg tgcaaatagt ataacaatca cttcctgctg aattgattca ggtcaggcca
    50701 aatggcggta ttttatacac aaaatgcggg tctggctctc ttttatactg attatgaaag
    50761 catagaccgt ttaccctccc tgggtacgac ggaacagggg tcctgatgga taatcacatc
    50821 cgatcccgga aaacgccgta aaatagcctg ctctacctga tccgccacca tatgtgcctg
    50881 aaccaaaggc agagagtctt ccatttccaa atgaatctga ataaagcggg tcggccctga
    50941 ctgccgcgtg cgaagatcgt gagcgccgct aacacccggc caggaagtca cgatatcaat
    51001 aatttcttgc cgttcttcat caggcaatgc gcgatccagt aatgactgta ccgcctcata
    51061 tcccatgcgt aacgcgctat ataaaatata gatgccgatt cccaatgcaa acagagcatc
    51121 ggcgcgatgc cagccgtacc aagacaaccc cagcgccagc agaattgcgc cgttcatcat
    51181 aacatcagac tggtaatgta gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac
    51241 cacccaccgc tgaaacgaga caaggataat cgtacaaatt agcgccacaa ttgtcacgat
    51301 aaccccgacg cctggatctg tcatcggtgt tggagatacc agatgttgaa tacccgtcaa
    51361 aaacaggaat agtgccgaac cggagataaa catactttgc gccagcgccg cgagggactc
    51421 agctttaccg tgaccaaacg agtgattatc gtcggcaggt tgcagggaat atcgcaccac
    51481 cagtaaattc gtcaacgacg cgccgatatc caccagcgaa tccaccagcg cggcgagaat
    51541 actcaccgac ccggtatacc accatgcaaa aattttaatc agcaatagca acgaagccat
    51601 cgccgtcgca gcaatcgccg cccgactgac cagccgtcca taagattgat tcataaatac
    51661 tcccgctatc aactgacgct agtataacgg aagcaaatca tctgcaatgc attaagcagc
    51721 aggcaaattg aggataaaaa aaaccccaca tcatgtgggg gaagacaggg atggtgtcta
    51781 tggcaaggaa aacagggttt actactggga acgtgagttg ctactactca atagcttcaa
    51841 cgatgaactt ttttgccatt gcgtcacgtc acgcaactgc tccattcgtt gttgatgttt
    51901 ctcgtttaaa accgcttgct gctccggcgt taacaggcga tacatttggt tgcggacttt
    51961 tgccatctca acctgacgag caatttgctc attcgccatt ttttctgcct gtgcgcgcac
    52021 agcgttttca tcaaaatttt ctgcggtgac aaggcgatgc attgtctcca gttcgctaac
    52081 attaacagga ggctgttcgt gccgggcctg ttgcataaga tctcgcatct gctgacgctg
    52141 atgttcggtt aaacttatgc cgtcgaacat atggctctgc gtactgcgct gcgtaagttc
    52201 ttcacccgga tgccagttat cgcctgaacc gacttcagca gcgtggctta atgaactgac
    52261 tgccagcgtt gaggccatga cggcagcggt aactatgcgc atcatttgct cccaaaatct
    52321 ttctgtcgcg attcaacgat agagagttta cgattcaggc tgcaaacatg cgtcaggggg
    52381 tgtaaaacaa cgtaaagtca tggattagcg acgtctgatg acgtaatttc tgcctcggag
    52441 gtatttaaac aatgaataaa atcctgttag ttgatgatga ccgagagctg acttccctat
    52501 taaaggagct gctcgagatg gaaggcttca acgtgattgt tgcccacgat ggggaacagg
    52561 cgcttgatct tctggacgac agcattgatt tacttttgct tgacgtaatg atgccgaaga
    52621 aaaatggtat cgacacatta aaagcacttc gccagacaca ccagacgcct gtcattatgt
    52681 tgacggcgcg cggcagtgaa cttgatcgcg ttctcggcct tgagctgggc gcagatgact
    52741 atctcccgaa accgtttaat gatcgtgagc tggtggcacg tattcgcgca atcctgcgcc
    52801 gttcgcactg gagcgagcaa cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg
    52861 atgccttagt gctgaatcca ggccgtcagg aagccagctt cgacgggcaa acgctggagt
    52921 taaccggtac tgagtttacc ctgctctatt tgctggcaca gcatctgggt caggtggttt
    52981 cccgtgaaca tttaagccag gaagtgttgg gcaaacgcct gacgcctttc gaccgcgcta
    53041 ttgatatgca catttccaac ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt
    53101 ggtttaaaac cttgcgtggt cgcggctatc tgatggtttc tgcttcatga taggcagctt
    53161 aaccgcgcgc atcttcgcca tcttctggct gacgctggcg ctggtgttga tgttggtttt
    53221 gatgttaccc aagctcgatt cacgccagat gaccgagctt ctggatagcg aacagcgtca
    53281 ggggctgatg attgagcagc atgtcgaagc ggagctggcg aacgatccgc ccaacgattt
    53341 aatgtggtgg cggcgtctgt tccgggcgat tgataagtgg gcaccgccag gacagcgttt
    53401 gttattggtg accaccgaag gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat
    53461 tcgtaacttt attggtcagg ccgataacgc cgatcatccg cagaagaaaa agtatggccg
    53521 cgtggaactg gtcggtccgt tctccgtgcg tgatggcgaa gataattacc aactttatct
    53581 gattcgtccg gccagtagtt ctcaatccga tttcattaac ttactgtttg accgcccgct
    53641 attactgctg attgtcacca tgttggtcag tacgccgctg ctgttgtggt tggcctggag
    53701 tctggcaaaa ccggcgcgta agctgaaaaa cgctgccgat gaagttgccc agggaaactt
    53761 acgccagcac ccggaactgg aagcggggcc acaggaattc cttgccgcag gtgccagttt
    53821 taaccagatg gtcaccgcgc tggagcgcat gatgacctct cagcagcgtc tgctttctga
    53881 tatctctcac gagctgcgca ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg
    53941 ccgtcgtagc ggtgaaagca aggaactcga gcgtattgaa accgaagcgc aacgtctgga
    54001 cagcatgatc aacgatctgt tggtgatgtc acgtaatcag caaaaaaacg cgctggttag
    54061 cgaaaccatc aaagccaacc agttgtggag tgaagtgctg gataacgcgg cgttcgaagc
    54121 cgagcaaatg ggcaagtcgt tgacagttaa cttcccgcct gggccgtggc cgctgtacgg
    54181 caatccgaac gccctggaaa gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc
    54241 ccatacgaag attgaagtgg gctttgcggt agataaagac ggtatcacca ttacggtgga
    54301 cgacgatggt cctggcgtta gcccggaaga tcgcgaacag attttccgtc cgttctatcg
    54361 gaccgatgaa gcacgcgatc gtgaatctgg cggtacagga ttggggctgg cgattgttga
    54421 aaccgccatt cagcagcatc gtggctgggt gaaggcagaa gacagcccgc tgggcggttt
    54481 acggctggtg atttggttgc cgctgtataa gcggagttaa actccgcatt tgtaggcagg
    54541 ataaggcgtt tacgccgcat ccggcatttg agcaggatgc ctgatgcgac gctgatagcg
    54601 tcttatcagg cctacactcc gggggttatt ttccccacaa ccggcgggaa aaatcttcga
    54661 tcttgccgct cagtcggcgc ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg
    54721 cggagagtaa acggtgatac tgatcgtcat caaacggcat atgccatgcg atggcggcag
    54781 cctcctgcac ggtcacatcg ctgacacggg aaaccaactc cagcggcgcg tccgcagaca
    54841 ctttccctgg cgctatcacg ctatacaacc accccacctt acctgtgttt tgcatcaact
    54901 gcgctatatc gctgatatca aaatggtaat tgagtttata gcaaggcgag cgcggctggc
    54961 tgacctgaat taatgcctct ccccagcgga aaatatcgcc catatgaaca ttactttccg
    55021 tgagaccgtc ggttgagaga ttttcgccaa acgcaggcgc aacaaacaac tccgc
//
LOCUS       DAJTRB010000015        53525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTRB010000015 DAJTRB010000000
VERSION     DAJTRB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.015"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..228)
                     /locus_tag="OO330_000995"
     CDS             complement(<1..228)
                     /locus_tag="OO330_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5881950.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAY"
     gene            complement(225..1151)
                     /locus_tag="OO330_000996"
     CDS             complement(225..1151)
                     /locus_tag="OO330_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5881951.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            1260..1922
                     /gene="can"
                     /locus_tag="OO330_000997"
     CDS             1260..1922
                     /gene="can"
                     /locus_tag="OO330_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5881952.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(1963..2499)
                     /gene="hpt"
                     /locus_tag="OO330_000998"
     CDS             complement(1963..2499)
                     /gene="hpt"
                     /locus_tag="OO330_000998"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5881953.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            2705..5095
                     /gene="gcd"
                     /locus_tag="OO330_000999"
     CDS             2705..5095
                     /gene="gcd"
                     /locus_tag="OO330_000999"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5881954.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(5142..6692)
                     /gene="cueO"
                     /locus_tag="OO330_001000"
     CDS             complement(5142..6692)
                     /gene="cueO"
                     /locus_tag="OO330_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5881955.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKRPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            6858..7205
                     /locus_tag="OO330_001001"
     CDS             6858..7205
                     /locus_tag="OO330_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5881956.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            7311..8177
                     /gene="speE"
                     /locus_tag="OO330_001002"
     CDS             7311..8177
                     /gene="speE"
                     /locus_tag="OO330_001002"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5881957.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            8193..8987
                     /gene="speD"
                     /locus_tag="OO330_001003"
     CDS             8193..8987
                     /gene="speD"
                     /locus_tag="OO330_001003"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5881958.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(9025..9387)
                     /locus_tag="OO330_001004"
     CDS             complement(9025..9387)
                     /locus_tag="OO330_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5881959.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(9562..12159)
                     /gene="acnB"
                     /locus_tag="OO330_001005"
     CDS             complement(9562..12159)
                     /gene="acnB"
                     /locus_tag="OO330_001005"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5881960.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            12514..14367
                     /locus_tag="OO330_001006"
                     /pseudo
     CDS             12514..14367
                     /locus_tag="OO330_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(14438..15862)
                     /gene="lpdA"
                     /locus_tag="OO330_001007"
     CDS             complement(14438..15862)
                     /gene="lpdA"
                     /locus_tag="OO330_001007"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5881961.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(16187..18067)
                     /gene="aceF"
                     /locus_tag="OO330_001008"
     CDS             complement(16187..18067)
                     /gene="aceF"
                     /locus_tag="OO330_001008"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5881962.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(18082..20745)
                     /gene="aceE"
                     /locus_tag="OO330_001009"
     CDS             complement(18082..20745)
                     /gene="aceE"
                     /locus_tag="OO330_001009"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5881963.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(20906..21670)
                     /gene="pdhR"
                     /locus_tag="OO330_001010"
     CDS             complement(20906..21670)
                     /gene="pdhR"
                     /locus_tag="OO330_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5881964.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            22211..23584
                     /gene="aroP"
                     /locus_tag="OO330_001011"
     CDS             22211..23584
                     /gene="aroP"
                     /locus_tag="OO330_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5881965.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(23627..24481)
                     /gene="ampE"
                     /locus_tag="OO330_001012"
     CDS             complement(23627..24481)
                     /gene="ampE"
                     /locus_tag="OO330_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5881966.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(24478..25029)
                     /gene="ampD"
                     /locus_tag="OO330_001013"
     CDS             complement(24478..25029)
                     /gene="ampD"
                     /locus_tag="OO330_001013"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5881967.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            25117..26010
                     /gene="nadC"
                     /locus_tag="OO330_001014"
     CDS             25117..26010
                     /gene="nadC"
                     /locus_tag="OO330_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5881968.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            26213..26653
                     /gene="ppdD"
                     /locus_tag="OO330_001015"
     CDS             26213..26653
                     /gene="ppdD"
                     /locus_tag="OO330_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5881969.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            26663..27810
                     /gene="tadA"
                     /locus_tag="OO330_001016"
                     /pseudo
     CDS             26663..27810
                     /gene="tadA"
                     /locus_tag="OO330_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            27800..29002
                     /gene="hofC"
                     /locus_tag="OO330_001017"
     CDS             27800..29002
                     /gene="hofC"
                     /locus_tag="OO330_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5881970.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIIGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(29037..30080)
                     /gene="guaC"
                     /locus_tag="OO330_001018"
     CDS             complement(29037..30080)
                     /gene="guaC"
                     /locus_tag="OO330_001018"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5881971.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(30242..30280)
                     /locus_tag="OO330_001019"
     CDS             complement(30242..30280)
                     /locus_tag="OO330_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5881972.1"
                     /translation="MIIKTLKMSARK"
     gene            30305..30925
                     /gene="coaE"
                     /locus_tag="OO330_001020"
     CDS             30305..30925
                     /gene="coaE"
                     /locus_tag="OO330_001020"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5881973.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            30925..31668
                     /gene="zapD"
                     /locus_tag="OO330_001021"
     CDS             30925..31668
                     /gene="zapD"
                     /locus_tag="OO330_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5881974.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            31678..31875
                     /gene="yacG"
                     /locus_tag="OO330_001022"
     CDS             31678..31875
                     /gene="yacG"
                     /locus_tag="OO330_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5881975.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(31975..32364)
                     /gene="mutT"
                     /locus_tag="OO330_001023"
     CDS             complement(31975..32364)
                     /gene="mutT"
                     /locus_tag="OO330_001023"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5881976.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRNITLWFWLVESWEGVPWGKEGQPG
                     EWMSLIGLNADDFPPANEPVIAKLKRL"
     gene            complement(32424..35129)
                     /gene="secA"
                     /locus_tag="OO330_001024"
     CDS             complement(32424..35129)
                     /gene="secA"
                     /locus_tag="OO330_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5881977.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(35191..35703)
                     /gene="secM"
                     /locus_tag="OO330_001025"
     CDS             complement(35191..35703)
                     /gene="secM"
                     /locus_tag="OO330_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5881978.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(35934..36851)
                     /gene="lpxC"
                     /locus_tag="OO330_001026"
     CDS             complement(35934..36851)
                     /gene="lpxC"
                     /locus_tag="OO330_001026"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5881979.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(36952..38103)
                     /gene="ftsZ"
                     /locus_tag="OO330_001027"
     CDS             complement(36952..38103)
                     /gene="ftsZ"
                     /locus_tag="OO330_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5881980.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(38164..39426)
                     /gene="ftsA"
                     /locus_tag="OO330_001028"
     CDS             complement(38164..39426)
                     /gene="ftsA"
                     /locus_tag="OO330_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5881981.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(39423..40253)
                     /gene="ftsQ"
                     /locus_tag="OO330_001029"
     CDS             complement(39423..40253)
                     /gene="ftsQ"
                     /locus_tag="OO330_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5881982.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(40255..41175)
                     /gene="ddlB"
                     /locus_tag="OO330_001030"
     CDS             complement(40255..41175)
                     /gene="ddlB"
                     /locus_tag="OO330_001030"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5881983.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRSEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(41168..42643)
                     /gene="murC"
                     /locus_tag="OO330_001031"
     CDS             complement(41168..42643)
                     /gene="murC"
                     /locus_tag="OO330_001031"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5881984.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(42697..43764)
                     /gene="murG"
                     /locus_tag="OO330_001032"
     CDS             complement(42697..43764)
                     /gene="murG"
                     /locus_tag="OO330_001032"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5881985.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(43761..45005)
                     /gene="ftsW"
                     /locus_tag="OO330_001033"
     CDS             complement(43761..45005)
                     /gene="ftsW"
                     /locus_tag="OO330_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5881986.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(45005..46321)
                     /gene="murD"
                     /locus_tag="OO330_001034"
     CDS             complement(45005..46321)
                     /gene="murD"
                     /locus_tag="OO330_001034"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5881987.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(46324..47406)
                     /gene="mraY"
                     /locus_tag="OO330_001035"
     CDS             complement(46324..47406)
                     /gene="mraY"
                     /locus_tag="OO330_001035"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5881988.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(47400..48758)
                     /gene="murF"
                     /locus_tag="OO330_001036"
     CDS             complement(47400..48758)
                     /gene="murF"
                     /locus_tag="OO330_001036"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5881989.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(48755..50242)
                     /gene="murE"
                     /locus_tag="OO330_001037"
     CDS             complement(48755..50242)
                     /gene="murE"
                     /locus_tag="OO330_001037"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5881990.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(50229..51995)
                     /gene="ftsI"
                     /locus_tag="OO330_001038"
     CDS             complement(50229..51995)
                     /gene="ftsI"
                     /locus_tag="OO330_001038"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5881991.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(52011..52376)
                     /gene="ftsL"
                     /locus_tag="OO330_001039"
     CDS             complement(52011..52376)
                     /gene="ftsL"
                     /locus_tag="OO330_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5881992.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(52373..53314)
                     /gene="rsmH"
                     /locus_tag="OO330_001040"
     CDS             complement(52373..53314)
                     /gene="rsmH"
                     /locus_tag="OO330_001040"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5881993.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(53316..>53525)
                     /locus_tag="OO330_001041"
     CDS             complement(53316..>53525)
                     /locus_tag="OO330_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="MraZ; UPF0040; crystal structure shows similarity
                     to AbrB; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5881994.1"
                     /translation="CQMDGAGRLLIAPVLRQHAGLTKEVMLVGQFNKFELWDETTWHQ
                     QVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        12642 a        14959 c        13204 g        12720 t
ORIGIN      
        1 gtaggcgttg gtgatcaccg acatcatgat cagccccggt acgatgaact gcatatagct
       61 gaagccatgc atatcgccaa tacgcgaacc tatcaggtta ccgaagataa taaagtaaag
      121 ggtcatggtg atgactggcg gcaccagcgt ctgcacccag atacgcataa agcgatggat
      181 ctctttcgcc cagatgcttt ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct
      241 tgtttttcat taaccagtga aacaaacagc tcttccagac ggttagcttt gttacgcata
      301 cttaatacct gaatgccctg ctcacttaac tgcgtaaata cgctgttgat cccctgctca
      361 cgtagcactt caacttccag cgtcgcggta tcgaccagtc gatactgata gccatcgagc
      421 ttcggtaacg gacttttcgg tgcgagatcg agaataaagg tttccgattt cagcttcgcc
      481 agcagcgcct tcatcgaggt attttccacc agctcaccat gttgaataat gccgatattg
      541 cggcacagca tttctgcttc ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg
      601 tcgtttaaat ccttcaaaaa gccccacatt gagcggcgaa gttcaatatc cacgcctgcg
      661 gtcggttcgt cgagaatcag tagtttaggt tcatgcatta acgcacgggc aatcattaaa
      721 cggcgcttca tcccgccaga taacatacgc gcacgttcgt tgcgttttcc ccataaatcg
      781 agctgcttaa gatatttttc gctgcggatg tacgcctctt tgcgctccac accgtagtac
      841 cctgcctgat tcaccacaat ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc
      901 ggcaccagtc ccaactgacg tttagcgttc acgacatctt tctcgagatc gtaaccaaat
      961 acgctgaccc gcccggaggt tttatttacc agagagctga taataccgat agtggtcgat
     1021 ttcccggccc cgttcggccc gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct
     1081 attccacgaa gcgcctgaac gccgcctgga tagttttttt taagctgttg aagttccagt
     1141 gcaatggtca taaattttta cttaccttac gttcttacac tttatctgtg gtttaaatcg
     1201 tcccggagtt gccctatatt agccaaacgt aattatttgg ttacaggtcg ttaacctcca
     1261 tgaaagacat agatacactc atcagcaaca atgcactatg gtcaaaaatg ctggtggaag
     1321 aggatcccgg gttttttgag aaactggcac aagcgcaaaa accgcgcttt ctatggattg
     1381 gatgttccga cagtcgcgtt cctgcagaac gtttaaccgg tcttgagccg ggcgaactct
     1441 ttgttcaccg taatgttgct aacctagtca ttcacaccga cctgaactgc ctttccgtgg
     1501 ttcagtatgc agtggatgta ctcgaagttg aacacattat tatctgtggc cactacggtt
     1561 gcggcggcgt acaagccgcc gttgaaaacc cggaactggg gcttatcaac aactggctgc
     1621 tgcatatccg cgatatctgg ttcaaacata gctcattgct cggcgaaatg ccgcaagagc
     1681 gccgtctgga taccttgtgt gaactgaacg tcatggaaca ggtgtataac ctgggccact
     1741 ccaccattat gcaatcagcg tggaaacgcg ggcagaaagt taccattcac ggctgggcct
     1801 acggcattca cgacggcttg ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc
     1861 ttgagcaacg ttaccgtcac gggatttcca acctcaagct gaaacacgcc aaccacaaat
     1921 aaaaatgcca tgccggatgc aacacatccg gcaacttcac acttactcgt ccagcagaat
     1981 cactttgcca atatacggca gatgacggta acgctgtgcg taatcaatgc cgtaacccac
     2041 cacaaactca tccgggatcg agaaaccgat aaattcaacc gggacgttca cttcacgacg
     2101 ggacggttta tccagcagcg tacaaatcgc caacgacttc ggttcgcgca ggcttaagat
     2161 ctcacgcact ttcgacagtg tattccccga gtcgatgata tcttcaacaa tcagcacgtc
     2221 cttgccacgg atatcttcat ccagatcttt gaggattttc acatcacggg tggtggacat
     2281 gccgctaccg tagctggagg cggtcataaa gtcgacttca tgagatacct gaacttcacg
     2341 gcacaggtcc gccataaaca taaatgagcc acgcagcaga cccaccagca ccatatcgct
     2401 gccgctgtct ttgtaacgct cagtaatctg acgacccagt tcggcgatac gcgctttaat
     2461 ctccgcttcg gggatcatta cttctacagt atgtttcata tctctaacca tatgatttaa
     2521 aaacaaatca cttcgcacgg gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta
     2581 taccagtaaa actattcatt cgcggaatct gttaataaag ctcgaattgt gatgacggtc
     2641 acacatgtta aaacctataa ttaattgcta ctaaaatatt aatgaattga aatggtgtct
     2701 ctttatggca attaacaata cagcctcgcg acgattactc gtcacgctaa cagccctttt
     2761 tgcagcgctt tgcgggctgt atctactcat tggcggaggc tggctggtcg cgattggcgg
     2821 ctcctggtac taccctatcg ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg
     2881 cagtaaacgc gccgcgcttt ggctatacgc agccctgctg ctcggcacca tgatttgggg
     2941 cgtctgggaa gttggtttcg acttctgggc gctgactccg cgcagcgata ttctggtctt
     3001 cttcggcatc tggctgatcc tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg
     3061 cgcagttgcc gcactggtgg tcgcactgct gattagcggt ggtatcctga cctgggccgg
     3121 atttaacgat ccgcaggaga tcaacggcac cttaagcgcc gatgccacac ctgccgaagc
     3181 tatctccccc gttgccgatc aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg
     3241 cttttcacca ctgaagcaaa ttcacgccga taacgtccac aagctgaaag aagcctgggt
     3301 gttccgtact ggcgatgtga agcagccgaa cgatccgggt gaaatcacca atgaagtgac
     3361 gccgattaaa gtgggcgata ccctttacct gtgtaccgct caccagcgcc tgtttgcgct
     3421 tgatgccgcc agcggcaaag agaaatggca ttacgatcct gagctgaaaa ccaacgagtc
     3481 tttccagcac gtaacctgcc gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc
     3541 gccggaagtg atggcggatt gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct
     3601 gattgcgatt aacgctgaaa acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct
     3661 caatctgcaa agcaatatgc cagacaccaa accgggtctg tatgagccga cttctccgcc
     3721 aattatcacc gataaaacca tcgtgatggc cggttcagtc accgataact tctcaacccg
     3781 cgaaacgtct ggcgtgatcc gtggttttga tgtcaacacc ggggagctgc tgtgggcttt
     3841 tgatccgggc gcgaaagatc cgaacgcaat cccgtcagac gaacacacct ttacctttaa
     3901 ctcaccaaac tcgtgggcac cagcggccta tgacgcgaag ctggatctgg tctatctgcc
     3961 gatgggcgtg accacgccgg atatctgggg cggtaaccgc acaccggaac aggaacgtta
     4021 tgccagctcg attctggcgc tgaatgccac taccgggaaa ctggcgtgga gctaccagac
     4081 cgttcaccac gacctgtggg acatggatct tccggcacag ccgacgctgg cggacatcac
     4141 cgttaatggt cagaaagtgc cgattattta cgctccggcg aaaaccggta acatttttgt
     4201 gctcgatcgt cgtaatggcg aactggtggt tccggccccg gaaaaaccgg ttccccaagg
     4261 tgctgccaaa ggcgattacg ttacccctac tcaaccattc tcggaactga gcttccgtcc
     4321 gacgaaagat ttgagcggtg cggatatgtg gggagccacc atgtttgacc aactggtgtg
     4381 ccgcgtgatg ttccaccaga tgcgctatga aggcattttc acgccgccat ctgaacaggg
     4441 tacgctggtc ttcccgggta acctggggat gttcgaatgg ggcgggattt ccgttgatcc
     4501 aaatcgtgaa gtggcaattg ccaacccaat ggcactgccg tttgtttcga aactgctccc
     4561 gcgtggtcct ggcaacccga tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc
     4621 cggcattcag ccacagtacg gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc
     4681 atttggtctg ccatgtaaac agccagcatg gggttatatc tcggcgctgg atctgaaaac
     4741 taatgaagtg gtgtggaaga aacgtattgg tacgccgcag gacagtatgc cgttcccgat
     4801 gccggttccg gtgccgttca atatgggtat gccgatgctg ggcgggccaa tctccacggc
     4861 gggtaacgtg ctgtttatcg ccgctacggc agataactac ctgcgcgctt acaacatgag
     4921 caacggtgaa aaactgtggc agggtcgttt accggcgggt ggtcaggcaa caccgatgac
     4981 ctatgaagtg aatggcaagc agtacgtggt gatctccgca ggcggtcacg gttcatttgg
     5041 tacgaagatg ggcgactata ttgtggctta tgccctgccg gatgatgtga aataaccata
     5101 atgcccggag agatccgggc atatttccga atacggtctt tttataccgt aaaccctaac
     5161 atcatccccg tatcttcatg ctccagcaga tggcagtgcg ccatataagc atgttctttc
     5221 ggtgcgttgt gattaaactt caccagcact tcactgacat tgccttctac cttaacggta
     5281 tctttccagc ccgcgcgatg agccgctggc cgtttgccat tttctgacaa gatacggaac
     5341 tgcgtgccgt gaatatggaa cggatgcagc atcatgtcgc ccacgccaga gataacccaa
     5401 cgttcgtact gccctttcgc cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg
     5461 ttgattttat tggcatggtg gaaatcgaac ttcccgccgt ggttcatatg attcatattg
     5521 ccgtgcccca tatggcccat catctggctg tgatccattc cggccatcgc ctgatcgcca
     5581 tatttctcca ttagcatctg catccccatc atatcgagca tcgggtccat agaaagttgc
     5641 agcttgcgta ccgtcagccc ttccagcgaa ggtaacgcag gcaggctact taatgtgtca
     5701 ggcaaagcac cggaggcact aatagcaatc ggctgaatcc gcattaccgg atgcggcttg
     5761 tcaaacggtg caatcgccat ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt
     5821 ttgttgtcgt taacctccac cagcacttca aaacgctcgc ccatcagcac cggcagctcg
     5881 ctcaccttca ccggttcagg tagcagacca ccgtcgctgg caatcacata cagcgggcga
     5941 ttgtcgctgg tggcgaaatt gagcgagcgg gcattacagc cattgagcaa acgcaggcgc
     6001 agccaaccac gcggggcagc gtgttgtggg tagattgcac cgttggtcag cagcgtatcg
     6061 ccaaaccagc ccacggcggc ggtcatcaca tccagttgat aatcaatctg cccgtcggcg
     6121 ctaaatttct tatcctgaac gatcaccgga acatcatcga taccccactg ttttggcagc
     6181 attaatttca ggatctcgtc atcttcaatc accaccagac cagccagccc catcgccacc
     6241 tgtcgcccgg tcttgccatg ttgatgtgga tggaaccagc aggtagcggc aggttgatca
     6301 acgttcaacg tcaccgagcg cttgccacct ggcggaataa ttccctgtgg gccgccgtcg
     6361 acgtcacccg gtacttccag cccgtgccag tgcaacgtcg tctcttccgc cagttggttg
     6421 tagatatcaa ccgttaccgc tttgccgcgc tgtaatttca ccgccggccc cagcagattg
     6481 ccgttatagc cccaggtagt tgcagttttc tcgccaaagg tggactgacc tgcgccaata
     6541 gttaactgaa tgcgattacg ggcatcggtc gtgagcaaat cagggatcgg taacgttggg
     6601 cgttccgccg caaagactgc acggctccac agtggcaagg ctgaagccac acccagtgcg
     6661 acggaatatt tcaagaaatc acgacgttgc atagttattt ccttattctt aagcaggcga
     6721 aacaaaatca aacgttgagc atagtccttc cccttacggg aaggtcaagc cgcagacaca
     6781 atttaatcga caataatggt cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta
     6841 agcccctggt agaagcaatg aagacgtttt tcagaacagt gttattcggc agcctgatcg
     6901 ccgtttgcgc aaacagttac gcgctcagcg agtctgaagc cgaagatatg gccgatttaa
     6961 cggcagtttt tgtctttctg aagaacgatt gtggttacca gaacttatct aacgggcaaa
     7021 ttcgtcgcgc actggtcttt ttcgcccagc aaaaccagtg ggatctcagt aattacgaca
     7081 ccttcgacat gaaagctctc ggtgaagaca gctaccgcga tctcagcggc attggcattc
     7141 ccgtcgctaa aaaatgcaaa gccctggccc gcgattcctt aagcctgctt gcctacgtca
     7201 aataatccct gatacctttt tgtagaaata ttgaccgtgc atccgcggtc aatgttagct
     7261 attatgttgc gccctttttt tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa
     7321 aaaaacagtg gcatgaaacg ctacacgacc agtttgggca gtactttgcg gtagataacg
     7381 ttctgtatca tgaaaagacc gatcaccagg atctgatcat ttttgagaac gctgcatttg
     7441 gtcgcgtaat ggcgctggat ggcgtagtac aaaccaccga gcgcgacgag tttatctatc
     7501 atgagatgat gacccatgtt ccgctactgg cccacggtca cgcgaaacat gtgctgatta
     7561 tcggcggcgg cgacggtgcc atgctgcgtg aagtaacccg acataaaaac gttgagtcaa
     7621 tcacgatggt ggaaatcgat gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc
     7681 ataacgccgg tagctacgac gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt
     7741 tcgttaatca aaccagccag acctttgatg tcattatctc cgactgcacc gatcctatcg
     7801 gtcccggcga aagcctgttc acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc
     7861 ctggcggtat cttcgtcgca caaaacggcg tctgcttttt acagcaggaa gaagccatcg
     7921 acagccatcg caaactcagc cattacttca gcgacgttgg cttttatcag gcggcgatcc
     7981 cgacctatta cggcggtatc atgacttttg catgggcgac agataacgac gccttacgcc
     8041 atctctcaac cgaaattatt caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt
     8101 acaatccggc aatccatacg gcagcttttg ccttacctca gtatctgcaa gacgcactgg
     8161 cttcacagcc gtcctaagga gaagataaga aattgaaaaa actgaaactg catggcttta
     8221 ataatctgac caaaagtctg agtttttgta tttacgatat ctgctacgcc aaaactgccg
     8281 aagagcgcga cggttatatt gcttatatcg atgaactcta taatgccaac cgtctgaccg
     8341 aaatcctgtc agaaacctgt tccattatcg gggccaatat tcttaacatc gcccgccagg
     8401 attacgaacc acagggtgcc agcgtcacta ttctggtgag tgaagaaccg gttgacccga
     8461 aactcatcga caaaacagaa caccccggcc cactgccaga aacggtggtt gctcatctcg
     8521 ataaaagtca tatttgcgta catacctacc cggaaagtca tcctgaaggc ggtttatgta
     8581 ccttccgcgc cgatattgaa gtctctacct gcggcgtgat ttctccgctg aaggcgctga
     8641 attacctgat ccaccagctt gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt
     8701 ttactcgcga cattaacggt atgaagcact ttatcgacca tgagattaat tcgattcaga
     8761 actttatgtc tgacgatatg aaggcgctgt atgacatggt ggatgtaaac gtctatcagg
     8821 aaaatatctt ccataccaag atgttgctta aagagttcga ccttaagcac tacatgttcc
     8881 acaccaaacc ggaagattta accgacagcg agcgccagga aattaccgct gcgctgtgga
     8941 aagaaatgcg cgagatttat tacgggcgca atatgccagc tgtttaacgg ctctggcgga
     9001 gctcccaggc tccgccagat ttatttactt ctgctgcacg aaattgcggt aagccgccac
     9061 gacctgcaga aaatcctcaa cgccgcatag cgacaggctt tcttcgtcgt agtagttcat
     9121 cccctcttcc atttcatcgc cagcgaattc cagttgattg gcgcgaacca tcacctcttc
     9181 agcgtccatc cacagggtgt attcatgccc tgcccgttgc caggaccgtt cgctaccttt
     9241 cagtgcgtgc gctgcttgtt ccacttcatc aagcaaggcc aggttttctt tcacctcttc
     9301 attaaaccag tgcccgacca cttcatgccc catggacata cgcaccttta ccactccggt
     9361 aatatcgcgc agaaattcgt aatccatagt gtgttcctct gctcccggca atgcgccgaa
     9421 atcgcatcgc gccattgatg taattatcgc agccttaccg gagaaaaaca gcggaacggc
     9481 gatagtttag aaataaaaaa tgcccgggaa tctcattcac cgggcattgt gtcgtttatg
     9541 cgcagcgcgt gcgcttactt attacaccgc agtctggaaa atcaccccat cggctttttc
     9601 ggtgtactga gaaagctggt tgaagttcag ataacgataa gtatcaacgg cagttttatc
     9661 tacttgcgca acgtaggtct ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc
     9721 aacagccgcc agttctgcag aagccaggaa gacattcgcg ccagtaccca aacggttcgg
     9781 gaagttacgg gtagaggtgg aaaccaccgt cgcaccgtct gctacacgcg cctggttacc
     9841 catacacagg gaacagcccg ggatctcgat acgagccccg ctcttaccga agacgctgta
     9901 gtagccttct tccgtcagtt gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg
     9961 ggtcggcaac tgacctttgt gcgcatccag cagtttacca gcggcacgga agtgaccgat
    10021 gttagtcatg caagaaccga taaatacttc gtcgatcttc tcgccctgta ccgcagacag
    10081 cggacgtgcg tcatccggat cgttcggagc acacaggatt ggctctttaa tctccgccag
    10141 atcgatgtcg atcactgccg cgtattccgc atctgcatcg gcttccagca gctcaggatt
    10201 cgccagccat ttttccatgc cctgaatacg gcgttccagg gtacgacgat cgccgtaacc
    10261 ttccgcgatc atccacttca gcaggacgat gttggagctc aggtattcga tgatcggttc
    10321 tttgttcagc ttgatagtac aaccagcggc agaacgctcg gcggacgcat cggttagctc
    10381 aaaggcctgc tcaactttca gatccggcag accttcaatt tccagaatgc gaccagagaa
    10441 gatgtttttc ttgcctttct tctcaacggt cagcagacct tgtttgatcg catacagcgg
    10501 aatggcgtgt accagatcgc gcagggtgat gcccggctgc attttgcctt tgaagcgcac
    10561 cagaacggat tccggcatat ccagcggcat tacgccagtt gcggcagcaa acgccaccag
    10621 accagaaccc gccgggaaag agataccgat cgggaaacgg gtatgggagt caccaccggt
    10681 accgacggta tccggcagca gcatacggtt cagccaggag tgaatgacgc cgtcacccgg
    10741 acgcagcgac acaccgccac ggttcataat gaagtccggc agcgtgtggt gcgtgttcac
    10801 gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga
    10861 gaagcccagg cacgccaggt ctttcagttc atcacgggtc atcgggccgg tggtgtcctg
    10921 agagcctaca gaagtcattt tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc
    10981 acaggcacgg cctaccattt tctgcgccag cgagaagccg cgatcgctct cagcgacatc
    11041 tttcgcctga cggaacacat cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt
    11101 caggccacgc ccgataatca gcggaatacg gccgccagca cgcacttcat caatcagcac
    11161 gtcggttttc agttcgaagg tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc
    11221 tttgtacggg taaacgtcaa tcacgtcgcc catgttcagg ttagagacgt tgacttcgat
    11281 tggcagtgca cccgcgtctt ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag
    11341 gcacaaacca ccgccgcgtt tgttcggcac atgtggaata tcatcgccca taaaccacag
    11401 aacggagttc gtggcggatt tacgcgatga acccgtaccc acaacgtcac cgacgtacgc
    11461 cagcgggaaa cctttctgtt gcagagcttc aatctgtttg atcgggccaa caacacctgg
    11521 ctggtctggc tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat
    11581 atccgggcgt gaccacgcat ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt
    11641 gactttaaag acggtaacgg tcagtttttc agccagcgcc gggcgattca ggaaccattc
    11701 ggcatccgcc caggactgca ttacctgctt cgcatattcg ttgcctgctt tcgctttctc
    11761 ttctacgtca tagaagttat cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat
    11821 aggtgccagt ttggcatcat ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg
    11881 catggtgccc agcagttcga tagctttttc cggagtcagc agaggggatt tagcttcgcc
    11941 tttagcgata gcagccagga agcctgcttt tacataggcg gcttcatcga cgcctggggg
    12001 aacacggttg gttaacagat ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag
    12061 cagctctaca agcgcggcca tttggtttgc atccaggggt ttgggcgcaa tcccctcagc
    12121 ggcacgctca gctacgtgct tacggtattc ttctagcacg acggttctcc tcgctctcat
    12181 tgtcatagtg cggcaggcga ttctcttcac gctcctgtga gacagcagtt tgtagggtaa
    12241 atgcccagta ccgcaacggg cagaatagca ggattttgac gacgtgttaa tctgtttaca
    12301 aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt gtttccctcc
    12361 gtaaaaactt cgttaattcg catggtgata atcacctttc tcaacgaatc ccaagattag
    12421 tcaaaattta aactaccgcc tctttatact cggattcaca gcacctgcgg gtggcagttc
    12481 gccgaccatt gcgatttcct tgagatccga attatgaaaa tgactttgcc gtttaaaccc
    12541 catgtgctgg cgctaatttg cagtgccggg ctttgtgctg cctctgccgg gctatatata
    12601 aaaagccgca cagtggaagc gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac
    12661 gctgccgctg ttacgcttcc tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc
    12721 aaatccgcat tcagcactgc acaaatagat caatgggtcg cacccgtcgc gctgtatccc
    12781 gacgccctac tttcgcaggt gctgatggca tcaacctatc cgacaaacgt tgctcaagca
    12841 gtgcaatggt cgcacgataa tccacttaaa caaggcgatg ctgctattca ggcggtatct
    12901 gaccagccgt gggacgccag cgttaaatca ctggtggcct ttccacaatt gatggcattg
    12961 atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag
    13021 gacgtgatgg actcggtaca gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag
    13081 tcatcaaccg aacagaaagt tattaccaca acgaagaaag ctgtaccggt aaaacagaca
    13141 gtcacggcac ccgtcatacc atccaatacc gttttaactg ccaaccccgt cattacagag
    13201 ccggcaacaa ccgtcatttc cattgagccc gccaatcctg atgtggtcta tattcccaac
    13261 tacaacccaa ccgtggttta cgggaactgg gccaatactg cgtatccgcc ggtttatctg
    13321 ccaccaccag ccggagaacc gtttgttgac agctttgtgc gcggattcgg ctatagcatg
    13381 ggcgttgcta ccacgtacgc actattcagc agcatcgact gagatgacga cgatcatgac
    13441 catcatcatc atgacgatga tgattatcat caccacgatg gcggtcatcg tgacagtaat
    13501 ggctggcaac acaacggcga caacatcaat atcgacgtca acaatttcaa ccgtatcacc
    13561 ggtgagcatc ttactgataa gaatatggca tggcggcaca atccaaacta ccgtaatggt
    13621 gtgccctatc atgatcagga tatggcaaag cggtttcatc aaaccgatgt caacggcgga
    13681 atgagtgcca cgcagctacc tgctccaaca cgcgacagcc agtgtcaggc ggcagcaagt
    13741 cagtttcagc aacgaacaca cgccgccccc gtcattacac gagataccca acgtcaggca
    13801 gcggcacagc ggtttaatga agctgaacac tatgggagct atgacgactt ccgcgacttc
    13861 agccgtcgcc aaccactgac ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg
    13921 gcctctcctg agcagcgcca ggcagtccgc gagaaaatgc agactaaccc gcagatccag
    13981 cagcgaaggg aggcggcgcg ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg
    14041 gttcgcgaga aaatgcagac taacccgcag aaccagcagc gaagagaggc agcgcgtgag
    14101 cgtattcagt ctgcaactcc cgaacagcgc caggtgttta aggaaaaagt acagcagcgc
    14161 ccactgaacc aacagcaacg tgataacgcc cgccagcgta tacaatcggc atcacctgaa
    14221 caacgtcagg tttttcggga gaaagttcag gagagccgcc cacaacgtct aaacgacagt
    14281 aaccatactg tcaggctgaa taacgagcaa cggtcagcag tacgcgaacg tctctctgaa
    14341 cgtggagcaa gaagactgga aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc
    14401 gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt
    14461 tcggcaggtc ggtaatgcta ccttcgaaca cttctgccgc caggcccaca gactcgtgca
    14521 gagtcgggtg cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc atttcgattg
    14581 ccaggccgat ttcacccagc agctcgccgc cgttggtacc aacaatcgca ccaccgatca
    14641 cacggtgaga ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag tcagaagcga
    14701 tagcacgacc agaagcagcc cacgggaagg tggcggtttc atagctgatg cctttctctt
    14761 tcgcttcttt ctcagtcaga cctacccatg caacttctgg ttcggtatag gcgatggacg
    14821 ggataacttt cggatcgaag tagtgtttct taccggcgat aacttcagcg gcaacgtgac
    14881 cttcgtgaac acctttgtgt gccagcatcg gctgaccgac gatatcgccg atagcaaaga
    14941 tgtgcggtac gttggtacgc agctgtttgt caacgcggat gaaaccacgg tcgtcaactt
    15001 ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg cacacgacca atcgctacca
    15061 gcacggcgtc gtaacgctgc ggttcagctg gtgctttttt gccttccatc gtcacataaa
    15121 taccgtcttc tttcgcttca acggcggtaa ctttggtttc cagcatcagg ttgaatttct
    15181 tgctgatacg cttggtgaag actttaacga tgtctttgtc agctgccggg ataacctggt
    15241 cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca
    15301 gaccgatgat accgccaccc attaccagca ggcgttctgg tacttctttc agttccagcg
    15361 cgtcagtgga gtcccagata cgcggatctt catgcggaat aaacggcagt tggatcgggc
    15421 gagaacccgc tgcaatgatc gcattgtcga agttgatcac ggttttgccg ttctcacctt
    15481 caacttccag ggtgttagcc ccggtgaatt tacccagacc gttgaccact ttgactttgc
    15541 ggcctttcgc cataccagcc agaccaccgg tcagctgatt gatcactttc tctttccagg
    15601 tacgaatctt gtcaatatcg gttttcggtt cgccgaagac gataccgtgt tcagccagcg
    15661 ctttggcttc ttcgataact tttgctacgt gcagcagtgc tttagaaggg atacagccga
    15721 cgttcaggca aacaccgcca agggtgttgt aacgttctac gattacggtt tccagaccta
    15781 aatcagcgca acggaaggca gcggagtaac ctgcggggcc tgccccaagt accacgacct
    15841 gagttttgat ttcagtactc atcatgacct ctatatattt atctccggcg gtcatacccg
    15901 tcgtctttca ggccgcagcg gcgttagctt cacgccctca ccccagtcac ttacttatgt
    15961 aagctcctgg ggattctggc gcttgctacc ttgctgcacc cagaaatcca tagggtatgt
    16021 gaagtaatac cctaaccacc accgggtcgt tctatccgtc ggtattttac aaaattgtta
    16081 acaattttta aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt
    16141 catgagatta ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac
    16201 ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt
    16261 cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt
    16321 taccattcca caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg
    16381 cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc
    16441 aaccgccctg catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca
    16501 tcagctcgcg agacagctcg atgatgcctt tcttgttgac gtctttgaat accggaacaa
    16561 ccagaccgtt cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac
    16621 gctgaccgtc ttccgacagc gaactattga agcgaggcat ctgctcaaga gctgcagcaa
    16681 cggctttcat gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt
    16741 cttcgttctg ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtggg
    16801 taacatgcgg gatcatcacc cagttacggc tcaggttagc accagaaatt ttctggatac
    16861 ggcccagttc cacttcttcg atttcgccaa acttgctgaa gtccaccttc ggccacggca
    16921 gcatgccagg gataccaccg ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt
    16981 ctttcacgta agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca
    17041 ctttcgccag gttaacgcca aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa
    17101 cgtaagcgtc gttttcagca aattcagatt tgccttccgc tttcgcagcc ggagcttcag
    17161 cttttgctgc cggagccggc gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg
    17221 cgccttcaac ttcgaagatc ataatcagcg aaccagtttt cactttatcg ccaacgttga
    17281 ctttcagttc cttcacgacg cctgcaaacg gagccggaac ttccatagaa gctttatcgc
    17341 cttctacggt aatcagtgac tgttcagcgg caactttgtc gcccactttc accatcactt
    17401 cggtcacttc aacttcgtca ccgccgatat ccggaacgtt aacttctttc acgccagctg
    17461 ctggtgcagg ggtcgctgcc ggagtcgctt cctgtttagc cgccggagct gccgcgcctg
    17521 cttcacccgc gacttcgaag accataatca gcgagccggt agacactttg tcacccacgt
    17581 tcactttgat ctctttcacg gtacctgcaa acggagccgg aacttccata gaagccttgt
    17641 cgccttctac ggtgatcagc gactgttcag cttcaacttt atcgccaact ttcaccagga
    17701 tttcggtcac ttcaacttcg tcgctgccga tatccggaac gttaacgtct tttgccgccg
    17761 cagccgcggg tgctgctgcc ggagctgctt ctttcttctc ttctgcctga gcaggtgcag
    17821 cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag tgcgccggtc tgggttttat
    17881 cgccaacaga gactttgatc tctttaacga tacccgcctg cggagatgga acttccatag
    17941 aggctttgtc gccttctacg gtgatcagcg actgttcggc ttcaactttg tcgcccactt
    18001 tgaccaggat ctcggtgatt tcaacttcat cagccccgat gtccggtact ttgatttcga
    18061 tagccattat tcttttacct cttacgccag acgcgggtta actttatctg catcgatgtt
    18121 gaatttggcg attgcgtcag caaccacttt cttatcgatt tcgccacgtt tagccagttc
    18181 gcccagcgcc gcaaccacga cataagaagc atcaacttcg aagtggtgac gcaggttctc
    18241 acggctgtcg gaacgaccga agccatcagt acccagtacg cgatagtcgt cagccggtac
    18301 gtaagtacgg acctgctcag cgaacagttt catatagtcg gtagatgcca ctgccggcgc
    18361 gtcgttcatc acctgagcga tatacggaac gcgcggagtt tccagcggat gcagcatgtt
    18421 ccagcgttca caatcctgac catcacgcgc cagctcggtg aaggaggtca cgctataaac
    18481 gtcagaacct acgccgtaat ctttcgccag gatctctgcc gcttcacgga cgtgacgcag
    18541 gatggaaccg gagcccagca gctgaacttt acctttgcta ccttcaatgg tttcgagttt
    18601 gtagatacct ttacggatac cttcctcagc accttccggc attgccggca tgtggtagtt
    18661 ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt ttttcaccgt acatacgctc
    18721 cagaccgtca tgcataatga cagcaacttc gtaagcgtaa gccgggtcgt aagagataca
    18781 gttcgggata gtcagcgact gaatgtggct gtgaccatct tcgtgctgca gaccttcacc
    18841 gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc
    18901 agccgcccag cacagatcgc cgatacgctg gaagccgaac atcgagtaat agatgtagaa
    18961 tgggatcatc ggcagattgt tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc
    19021 tgcgcccagc tcgttgatcc cttcctgcag aatctgacct ttctcgtctt ctttatagta
    19081 agcaacctgc tcgcggtcct gcggggtgta ctgctgaccg ttcgggctgt aaataccaat
    19141 ctgacggaac agaccttcca taccgaaagt acgcgcttcg tcggcgatga tcggtaccag
    19201 acgatctttg atcgacttgt tcttcagcat cacgttcaga gcacgaacga aagcgatagt
    19261 ggtagagagc tctttgctct gctcttccag cagcgcgccg aagtcttgca ggctcggcag
    19321 ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga taaccgtgca gtttctgacg
    19381 ctgagcgtgc agataggtat gctcttcaga accttccggg aaggtgatgt acggcagttt
    19441 ttcgatatct gcatcagaca ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc
    19501 catgttcatt ttcttaacct ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc
    19561 gtaaccttta atggtatgag caaggattac tgtcgctttg cctttggttt cctgcgcttt
    19621 cttgaatgca gcgtagattt tcttcggatc gtgaccacca cggttcagtg cccagatctg
    19681 ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga tatttaccga agaagtgttc
    19741 acgaacgtac gcaccatctt tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt
    19801 catcagctgg atcagtttac cgctggtatc tttacgcagc agttcatccc aacggctacc
    19861 ccacatcact ttgatcacgt tccagccagc accttcgaag atgccttcca gttcgttgat
    19921 gatcttgccg ttaccggtga ccgggccgtc aagacgctgc aggttacagt tgataacgaa
    19981 aaccaggtta tccagttttt cacgggtagc gatggtgatc gcacctttgg attccggttc
    20041 gtccatttca ccgtcgccga ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc
    20101 acggtgttcc agatatttca ggaatttagc ctggtaaata gcaccaatcg gacccagacc
    20161 catagatacg gtcgggaact gccagaattc cggcatcagt ttcgggtgcg gataggaaga
    20221 gaggccattg ccgtgaactt cctgacggaa gttatccagc tgctcctgag tcagacgacc
    20281 ttccaggaaa gcacgggcgt aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc
    20341 gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta aagcacacat cataaatggt
    20401 tgcggaagac tggaaggacg ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg
    20461 cagcaccgtc atgatggcgt tccagcggat agctgaacga atacggcgtt ccagttccag
    20521 attacccgga tactccggtt gttcttcaac ggggatggtg ttgatgtagt tgctgatacc
    20581 tgtgcctgcg gctacgttta caccgccttt gcgggcttca gcaagcagtt ggtcgatcag
    20641 atactgagca cgctcaacac cttcttcacg gatgaccgat tcgatcgcct ggagccagtc
    20701 gcgagtttcg atcggatcca cgtcatttgg gaaacgttct gacatgggtt attccttatc
    20761 tatctaataa cgttgagttt tctggaacct gtcccattga actctcgcca gaaagctcaa
    20821 taagacaggt tctacgttta gttaccgcgc ttttatatgc gcttgattta caacatcttc
    20881 tggataattt ttaccagaaa aatcactaat tctttcgttg ctccagacga cgcagagaac
    20941 gctcacggcg gctctcttca cggcttctgt cgagcaaaat ttcttcgata aaggccagat
    21001 ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc cataatcgct tcaaatatgc
    21061 gggtgcggtg actactcacc agcggcagca tctcgcgacg cgaatagagc aattcgaagt
    21121 tctggcggac attctgggcc aacatcggct ccatacacct tagcagatga agcagaacca
    21181 cattgtgggc cgcttcggtg acggcaatct gatactggag tacggcgttt gattccgcgt
    21241 ccagatcgcc agactgctgc gccagctcta tggcgtggtg gagttcacgg atgcgttcct
    21301 tgtcttcatc ggtactacgc agcgcggcgt aataagcggc gataccttcc agggcgtgtc
    21361 gtgtttcgag caagtcatac tgtgattcag gatggtcgga gagcagctcc accagcggat
    21421 cgctgaagct ttgccatagg ctgctctgga caaaagtgcc gccaccctgg cgacgaagca
    21481 acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga
    21541 actgttttgc cagttcgcgt tccggtggga gtttttcgcc cgggcggaga gtgccttcga
    21601 ggatcaaaaa ctccagttgc tgctcaatca catcggagag ttttggttgg cggattttgc
    21661 tgtaggccat gagttcctgt cttaagccac ttgccgaagt caattggtct taccaatttc
    21721 atgtctgtga cgctaaagta acaaagtatt caccttatgt ccatacaggt tttgattgaa
    21781 atcatgaaac tgtgcacatt ttaacaactt gacatatata acgtttcaaa gttgtaacta
    21841 tgcacaaatg tagactttac gtaggaaagg gattttttaa cgattattaa actataaaaa
    21901 tccgaattga accgattcac ttaccaattt tttgattttt aagtcaataa aaacgaattt
    21961 aaattcactc tgcatattga gaggggttga ggctgagctt tacaaacggt ttctttttaa
    22021 gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc tgccacatac cattattctt
    22081 gatctgacgg aagtcttttt gtaacaattc aaacttcctt gatgtaaata aatcaataca
    22141 acaaacggaa ttgcaaactt acacacgcat cactgcgtag atcaaaaaaa caatcaccgc
    22201 acgaggtttc atgatggaag gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa
    22261 ccgccatatt cagcttatcg cgctgggtgg cgcgataggg acagggctat tcctaggtag
    22321 cgcctccgta atacagtccg cagggccagg gattatcctg ggttacgcca ttgctggttt
    22381 tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg
    22441 ctcctttagc cactttgctt ataaatactg gggcagtttt gccggtttcg cctctggctg
    22501 gaactactgg gtactgtacg ttttagttgc catggcagag ctgactgccg tgggtaaata
    22561 cattcagttc tggtatccgg aaatcccaac ctgggtttct gccgccgtat tctttgtggt
    22621 gattaacgcc atcaacctga ccaacgttaa agtgtttggc gagatggagt tctggtttgc
    22681 cattatcaaa gttattgcgg tagtagcgat gatcatcttc ggcggctggc tgctgttcag
    22741 tggcaacggt ggcccgcagg cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc
    22801 gcacggcttc accggactgg tgatgatgat ggcgattatc atgttctcgt tcggtggtct
    22861 ggaactggtg gggatcaccg cagcagaagc tgataacccg gagcaaagta tcccgaaagc
    22921 aactaaccag gttatctacc gcatcctgat tttctatatt ggttcgttag ccgttctgct
    22981 ctcactgatg ccgtggaccc gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca
    23041 cgagttaggc gatacctttg tggcgaatgc gctgaacatc gtggtactga ctgcggcgct
    23101 ctccgtgtac aacagctgcg tatattgcaa cagccgtatg ctgtttggtc tggcacaaca
    23161 ggggaacgcg ccaaaagcgc tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat
    23221 tctggtgtct gcgctggtta cagcattgtg cgtattgatt aactatcttg ctccggaatc
    23281 cgcatttggc ctgttaatgg cactggtggt atccgcactg gtgatcaact gggcgatgat
    23341 cagtctggcg catatgaagt tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt
    23401 ccctgctctg ctttatccgc tgggtaactg gatctgcctg ctgtttatgg cggcggtact
    23461 ggtgattatg ctgatgaccc caggaatggc gatttcggta tacctgatcc cggtatggct
    23521 gatcgtgtta ggtatcggct atctgtttaa agagaaaacc gccaaagccg taaaagcgca
    23581 ttaatctctc tacgccctca cccgtacagg gtgagggcaa taatctctac accaacgccc
    23641 cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa cgaggttttc ttcgccattg
    23701 aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg ttcacgcgcc agagagaact
    23761 gcgccagacg cgttaatacc tgatattgcg aagtatggaa atcacccagc gaagcaaacc
    23821 aggccggtaa cgctttctca ccatgaccga tcaaggcata taccacaccc gcaagacgaa
    23881 ccggcaccca atccagtaca tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg
    23941 gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa gaacgcatac cccatcagcg
    24001 taacgggtcc ccaggttccc cccacaatca gccagaacag cggtgcaaga taaaaacgaa
    24061 agttaatcca cagcaatgca ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg
    24121 ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt ggcacgggca tggctatcat
    24181 tacgtgaagc agctgtcaga taagcatgat aatgaagacg aactttacct gcgccaatac
    24241 acagcaaacc aatcagcagc cacaccagca gcgtgggaac gttgaacaat actccctgca
    24301 atgcgcgtaa cagtaaaaaa gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc
    24361 cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag acgatgatca agctgccagt
    24421 gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag taaggttgta aatagcgtca
    24481 tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc
    24541 ggttttccga tccggcgcaa tatcacaatg gcccgtcatg ttattagcga tatcgggata
    24601 gcgatctatc agtgcccgcg taaccgccgc aagctgttga tactgcgcat cggtatacgc
    24661 cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa tcattgcagc gttcacgccc
    24721 ctgatactga gagactcccg catgccaggc acgtttatcg aaaggaacat actggactat
    24781 ttcaccatca cggcgaatca aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa
    24841 gaaaggatgt gcctgcggat caatagttcc agtgaataat gcgtcgatcc acggaccgcc
    24901 aaactcgcct ggcggcaggc taatattgtg caccaccagc agggtgggtg tttcgtcatc
    24961 caggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc
    25021 taacaacatg caggatctcc ttatatgtgg tgctaatacc cggttcagag tagcatgttt
    25081 ctaccttatg attcgttagc tatctggagt tttaacatgc cgcctcgccg ctacaaccct
    25141 gacacccgac gtgacgagct gctggaacgc attaatctcg atatccccgg cgcggtggcc
    25201 caggcgctgc gggaagattt aggcggaaca gtcgatgcca acaatgacat tacggcaaaa
    25261 cttttaccgg aaaattctcg ctctcatgcc acggtgatca cccgcgagaa tggcgtattt
    25321 tgtggcaaac gctgggttga agaggtgttt attcaactgg caggcgacga tgtcaccata
    25381 atctggcatg tggatgacgg cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa
    25441 ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt
    25501 tcaggagttg ccagtaaggt acgccactat gtcgaattac tggaaggcac caacacgcag
    25561 ttgttggata cgcgcaaaac cttacctggc ctgcgttcag ctctgaaata cgcggtactt
    25621 tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg ccttcctgat caaagaaaac
    25681 catattattg cctccggctc agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg
    25741 gatgcgccag tagaagtcga agtagagaat ctggaagaac ttgatgaggc cctgaaagcc
    25801 ggagccgata tcatcatgct ggataacttc gaaacagaac agatgcgcga agccgtcaaa
    25861 cgcaccaacg gcaaggcgct actggaagtg tctggcaacg tcactgacaa aacactgcgc
    25921 gaatttgccg aaacgggcgt ggactttatc tccgtcggtg cgctaactaa acacgtacaa
    25981 gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag atttatccgc tccatcgcgg
    26041 gcggataatc tccccttccc caactttctt cgtaacgcct cgcaaatttc ttgtctttcg
    26101 ctgcaacgaa ataaaaaacc tcggaatctg ctttcccgtt tcgttttttg cccctcgcct
    26161 gtgctgatgg cgtttgccaa agtagcacca accaaatcaa ggagcgaaac agatggacaa
    26221 gcaacgcggt tttacactta tcgaactgat ggtggttatt ggcatcattg ccattttaag
    26281 ctccattggt attcccgctt atcaaaacta cctgcgcaaa gccgcactca ccgacatgct
    26341 acaaaccttt gtgccttacc gtaccgccgt agagttgtgc gcgctggaac atggtggatt
    26401 agatacctgc gacggtggca gcaatggcat tccctcgcct accaccaccc gctatgtttc
    26461 agccatgagt gtggcaaagg gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct
    26521 aagcgtcgtc atgacgccag gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa
    26581 ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc gaagatgtct tccgctttga
    26641 tgacgccaac taaggagcgg caatgaatat tccacagctc acggccctgt gcctgcgtta
    26701 tcagggagtc ttgctggatg ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc
    26761 ctcacatgag ttgctggacg cattgcattt cgctaccacc aaacgtattg agatcacctg
    26821 ctggacgcgc caacaaatgg aaggtcacgc cagtcgcaca caacagacat tacccgtagc
    26881 tgttcaggag aagcatcagc ccaaagcaga attgctgact cgaacgttac aatctgcgct
    26941 ggaacaacgc gcgtctgata ttcatatcga accagcggac aatgcctacc gcatccgctt
    27001 gcgtatcgac ggcgtattgc atcctttacc ggatgtttca ccggatgccg gagtcgcatt
    27061 aacagccaga ttaaaagtgc tgggaaacct ggatattgcg gaacatcgcc tgccgcagga
    27121 cgggcaattc actgtcgaac tggcaggaaa caccgtctca tttcgtattg cgaccttacc
    27181 atgtcggggt ggtgaaaagg tggtattaag gttgttacag caggtgagcc aggcactgga
    27241 tgttaacacg ctgggaatgc agccgttaca actggcggac tttgctcatg ccttgcaaca
    27301 accacaggga ctggtgctgg taactggccc tacaggcagt ggcaaaacgg tcacctggtg
    27361 ttgtccacgc tacacaccaa ttccaccact gaaacgctgg tacgtttaca gcaaatgggg
    27421 gtcgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgt
    27481 aaactttgcc cacattgtcg ccgacagcaa ggggagccca tccatattcc agacactgta
    27541 tggccgtcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta ccacggtttt
    27601 tatggtcgta ccgccttgtt tgaagtcctg cccattacac cagccatacg ccagcttatt
    27661 tccgctaata ctgaagttga atcgctggaa acgcacgccc gacaggcggg tatgcgtacg
    27721 ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc
    27781 cgcgtactgg ggatgccgca tggcaagtaa gcaactctgg cgctggcatg gcataaccgg
    27841 cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtactttac tgcttatggc
    27901 actacagcaa cagatggtta ccccactaag cctgaagcga atcgccatca attctgcgca
    27961 gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac ttaaagccgg
    28021 gttaacgctt tctgaagggc tggctctgct ggcggaacag catcccagta agcaatggca
    28081 agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt
    28141 attaccctgg tcagaggcat ttccgccgct ctatcaggcg atgatccgca cgggtgaact
    28201 gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaat cccagcgtca
    28261 gttgaccgac aaagtgaaat cagcgttgcg ttatcccatc atcattttag cgatggcaat
    28321 catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac
    28381 cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg
    28441 cgaatggggc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca ataagttgct
    28501 gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca tcccgattat
    28561 aggttcactg atgaggggac aaaaactcac gcagatcttt acgattctgg cgctgacaca
    28621 aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa tgcgctgccc
    28681 gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg
    28741 gctggcgcta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag tgagaacagg
    28801 agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc atcgggataa
    28861 cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc tgatcataac
    28921 gggaggaatt atcggtacgc tggtggtggc aatgtatctg ccaattttcc atttaggcga
    28981 tgcgatgagt gggatgggat aacgctggcg tgttgctcca cgccagcgtt gggagattac
    29041 aggttgttga agatgcggtt ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc
    29101 agctctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata
    29161 tcacgcgcgg tattttcaac ctggcctcgc agcggcagct taacggtttt accttctgct
    29221 gcgcggtatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg
    29281 tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg
    29341 cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca
    29401 tcgcccggcg tggtacagcc accgtcgctg acgatcattc cgcccagacc gtgcgccgca
    29461 tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt gacgcgagtt
    29521 gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga gaggataagc
    29581 tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct
    29641 tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata
    29701 caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca
    29761 tcagacgtgc cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata
    29821 aacgcttgcc actcttcgac agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa
    29881 gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc gataatcggc
    29941 acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg
    30001 gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc
    30061 agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg
    30121 caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct
    30181 ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat
    30241 ttcactttcg cgcgctcatt tttaacgttt tgataatcat gatgaattcc cgggaatata
    30301 aattatgggg tatatagttg ccttaacggg aggcattggc agtggtaaga gtaccgttgc
    30361 caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt
    30421 ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg aaaacatgat
    30481 tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga
    30541 agagaaaaac tggcttaacg acctgctgca tccgctgatt cagcaagaga cgcaacacca
    30601 gatccagcaa gcaacttccc cctatgtact gtgggttgtg ccattgctgg tagaaaactc
    30661 actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa
    30721 gcgcaccatg cagcgcgatg atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca
    30781 ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc
    30841 ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca
    30901 gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat
    30961 gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta
    31021 cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga attactggat
    31081 gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt
    31141 aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt
    31201 cagcagttaa aagcggcggt gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg
    31261 cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc
    31321 agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgacagc
    31381 caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg
    31441 gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag
    31501 gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat
    31561 ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa
    31621 aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg
    31681 tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc
    31741 agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct
    31801 gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa
    31861 gagccaaagc agtgacattt gtctgatgcc tcacgtaggc ctgataagac gcggacagcg
    31921 tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacctacag
    31981 acgcttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagacc
    32041 gatcagcgac atccactcac cgggttgccc ttctttaccc cacggcaccc cttcccaact
    32101 ttcgaccagc cagaaccaca gagttatatt cctgtccggg aattcatatt ccagtttttc
    32161 aaatagcgaa aaatgttgtg gggtaatccc gacctcttcc tgaagttcac gcaccaccgc
    32221 ctgttccggc gtttcgccca tttcaatttt accgccggga aactccagtt tattcgccat
    32281 gtgcgcatct gctgcgcgac gtgttataaa gatttcattg ttctcgttgc gaataatacc
    32341 taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg
    32401 ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt gtatttttta
    32461 ccagaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt ttgcgccgcc
    32521 agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa
    32581 cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg catacgtacc
    32641 tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat cgctgcaaac
    32701 atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata gccacgcagg
    32761 tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag ggagtcaagc
    32821 gtttgcagca tgacgccttt ctcgaagtga cgcatcatct cagcaccaac cacttcttct
    32881 ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc
    32941 agttctggtt ctttatccag ccactcggca attggtaaat cgaggtcgaa atcgttcttc
    33001 agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgtgg cggaatgtag
    33061 gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc gctcacatcg
    33121 ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg
    33181 tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac tttacgctgg
    33241 gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctgg cttcataccc
    33301 agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc
    33361 atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac
    33421 tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct
    33481 tccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg
    33541 gtcggatttt ccagcgcggc aacttctgcc tgccagctac ctccgagcac aatatctgta
    33601 ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca
    33661 atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat accggctttg
    33721 gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc
    33781 tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca
    33841 gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt cggaacaacg
    33901 acggtatcca gtttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc
    33961 atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt
    34021 tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg
    34081 gaccagcgtc ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca
    34141 tctttaacga tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt
    34201 acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg
    34261 ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg
    34321 cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt
    34381 tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct
    34441 tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact
    34501 tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct gaacgccatg
    34561 ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg
    34621 taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccaac agtcaggcca
    34681 aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg
    34741 gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag cgttgcggtc
    34801 agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg
    34861 ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc
    34921 acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag acgcgcacga
    34981 aactctgcgg ttttcccttt cagttcttca tcggagagtt tttccatctc cggttccatg
    35041 gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg
    35101 aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg cgttacggag
    35161 ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc cagcacggat
    35221 gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc
    35281 ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag
    35341 cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag caacgggcag
    35401 tgtttgcggt gccattgcga aagaaagatg acggattacc gtacgaatag catgttgatg
    35461 ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa
    35521 ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc
    35581 gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa
    35641 taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact
    35701 cacgttattg ccatcccgat cgaattcaaa cgtatttagc gcacaaatct tatcattaaa
    35761 gcctgaagtc cacagcacga atgaagataa gttgtgcgta aaagtgctta agaaaacagc
    35821 gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg ttcacctggc
    35881 cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc
    35941 agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca
    36001 tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga
    36061 ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg
    36121 atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc
    36181 agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca
    36241 cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg
    36301 cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc
    36361 ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc
    36421 cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag
    36481 ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc
    36541 atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caggcccgcg
    36601 agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac
    36661 gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc
    36721 aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg
    36781 actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt
    36841 tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc
    36901 ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc
    36961 ttgcttacgt aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc
    37021 attgtcattc actactttag caaccggctt ctgctcctgg gtcagcggag ccatcccatg
    37081 ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc
    37141 aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct cgtcattcat
    37201 atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat
    37261 ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg tgatgttaac
    37321 cagcacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag agatagccat
    37381 ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta
    37441 gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg
    37501 agtaatcagt tcggcgatac cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa
    37561 cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt
    37621 gatcagagag tccacatgct tggacagttc agtgatcccc tgctcggcga atgccatacg
    37681 cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga tacccaaatc
    37741 ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc
    37801 agcaataaag accatgtctg caccttccag cgccgcacgc aatgcatcgc gatcctcatc
    37861 agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg tgataccgct
    37921 accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac
    37981 cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag cattaccgcc
    38041 gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa
    38101 catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa
    38161 aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc
    38221 tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag
    38281 caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt
    38341 cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc
    38401 ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc
    38461 ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc
    38521 ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc gcggcggacg
    38581 accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca
    38641 accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat
    38701 atcactggtc acgacattgc cagcataagg aattaccttt gtgtggcgca atgccccacc
    38761 ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc
    38821 acgttcatct tccgtcaata cagaataact tgatgccagt ccggcaaata tcagttggtc
    38881 aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtgaca
    38941 tgtgatcagg tgcacttttg cctgcatccg cacacccgaa agtcctaccg gattcttgat
    39001 cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgctcatc
    39061 gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt gcgtcacttc
    39121 ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag
    39181 cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcgcg
    39241 ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgtga
    39301 cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa
    39361 agcggcaacc ttcgcggtac caatttccag tcctactacc agttttctgt ccgtcgcctt
    39421 gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag
    39481 ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg
    39541 ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt
    39601 ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag
    39661 gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc
    39721 cccatttcgc gatagccctg caacacttca ttagcgctgc cttccgggcc atacagcatt
    39781 ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct
    39841 accatgtgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat
    39901 tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg ttgttctatt
    39961 tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccagg
    40021 atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa caccagcttt
    40081 gagagcggca ggcgttgcgc atcttccatc cagctcaaca cgacccagcc gctcaccaac
    40141 actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg
    40201 cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga catattagtc
    40261 cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc
    40321 catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa
    40381 ctgtccatca ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt
    40441 ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg
    40501 gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga
    40561 cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg
    40621 cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc
    40681 atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct
    40741 cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct tatcgctcag
    40801 gcctttttca aactctgagc gggttaacgc tacccacggc gcgaccggta aaccggcacc
    40861 ttgccacaga agtttgctgc gtagtttatc tattgaaagc gcagatgcca tcactccgct
    40921 gccggtataa ggcaagccca tcagctcgag catgccctgc agcgtaccat cttcaccgcc
    40981 gcgaccgtgt agcgcgataa acactttctg aaaacccatc gacttcagtt gcgtcacgtc
    41041 gacttctttc gggtcgacag gatacgcatc aataccgcct tcacgcagcc cggctaacac
    41101 cgctgcgcca gaattcagag aaacttcccg ctcagcggaa gtcccgccca acaggaccgc
    41161 gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta
    41221 aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg tcgttaccgg
    41281 ttaataccgg tgccagcatc tcggctacct gcgcagggtc cggcaccaga atgggatcaa
    41341 ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc ggaattggcg
    41401 cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg
    41461 cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac agcattacca
    41521 ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt
    41581 ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg
    41641 ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctctcc agcgcacgca
    41701 aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct gcggcgttca
    41761 gcgcgttatg acggcctggc gcattcaggg tgacgcgcat cggctctttg tcctgacgca
    41821 gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc acgtcggcat
    41881 cttcgctgaa gccgtaagtt gtggtctgac gtcccacacg cggtaacaat tcgcggatca
    41941 ccggatcatc aacacacatc accgcacgac cgtaaaaagg caggttgtgc agaaaattaa
    42001 taaaggtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga
    42061 tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg
    42121 cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca
    42181 gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg
    42241 cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta
    42301 actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa
    42361 tttccgggtt gtcggcagaa atggcgctgg aaacaacgac cacgctggca tcacgtacgt
    42421 tttccgggcg atggttgaaa taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg
    42481 gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa
    42541 taccgcccat accggcacca ccaatgccga caaaatgtat gtgccgaacg cgacgcattt
    42601 cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact
    42661 tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag cccggctcac
    42721 ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat
    42781 ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag
    42841 ctgtggctgc tcaataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta
    42901 ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat
    42961 ttcactcaca gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc
    43021 cgccatatca tcaataaatt cagtcacttt atgctgcggt tgccccgctt cggcatacgc
    43081 ctgttcaacg gattgttgcg aacctttgcc gctctgatgc cagatagtga ctgaatcacc
    43141 cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc
    43201 cactaccaac acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggcag
    43261 cgccagcaca tcggtacgca ccgggttacc caccacttcc gcattaggga aagcgcctgg
    43321 aaacgcctgc atcactttgg tagcaatctt cgccagccat ttattggtta agcccgcaat
    43381 accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccacctgg
    43441 acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt tcataatcgc
    43501 ccgcgcctga cgccaggcgt tgaagatacg cagcggcgca gctatcagtg cttttatacc
    43561 ttttccacgc aaaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa
    43621 gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag
    43681 atagtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat
    43741 taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga
    43801 cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc
    43861 gaaccaccgt aactgattag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc
    43921 gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa
    43981 ccggaaaaac ggtggtcaat ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg
    44041 acgaagaata ccattaaaag tgctagtacc acaccgacat aacctaattc ttcgccgata
    44101 atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg taccgaatta
    44161 cctaaacctt gcccccaaag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga
    44221 tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg
    44281 tacggttcgg cgaggatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg
    44341 aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc
    44401 accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc
    44461 atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg caccagatag
    44521 ttggcgatat agcaaaacag cgacagtttt gtcagctccg caggctggat acgcagcaaa
    44581 ccgagatcga tccaacgcga tgccccttta acagcgctac ccactaccag cacgatcatc
    44641 agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc
    44701 agacgcagcg taatgatcgc caaaataaac gccaaaatca gatagacacc atcacgcttc
    44761 gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc
    44821 atgataaagc caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg
    44881 atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccctttag cgccgtggag
    44941 atccagacca ggatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa
    45001 cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct
    45061 tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc ggctgaacac
    45121 gcggagccag caagcgcata gcctgttcca tagtttcggt ttgttctgcc acttccgggc
    45181 gtagcgccgc cagctgcgcg ccgtcacgac cgaaacaata cagacgtacg ttatcgccat
    45241 tcaggtaacg caccagtggg ctaaagtccg ccgatttacc atcgccgccc agcaacaaat
    45301 gcagcgtgcc gtctacgtgc aggccattca gcgccgcttc cgtactgccg acgttggtcg
    45361 cttttgaatc gttaatccag cgcacaccgt tatgctccag caccacttca aagcgatgcg
    45421 gcagaccggt gaaggtggtt aacgctttca ggctgctggc gcgcggtaac cctgcggcat
    45481 ctgccagcgc cagcgccacc agcgcattgg tgtagttatg ctgcccggaa agtttcatct
    45541 ctttcacatt cagcactttc tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat
    45601 tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa
    45661 tcggcattgt taaggcatca tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc
    45721 gcagttttgc tgcacgatac tgttgtaaac caaacgggta gcgatccata tgatcttcag
    45781 tcacgttcag aatggtcgct gctaccgcct gtaagctgga ggtggtttcc agctggaagc
    45841 tcgacagttc cagcacgtac agttcacact catcatccag tagcatcaac gcaggcaggc
    45901 caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg
    45961 tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc
    46021 gacaaaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg
    46081 atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc atcagccatt
    46141 catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc aggccaggcg
    46201 gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc
    46261 aggaaagccc ggtgaggccc aggccgataa tgacaacatt tttaccctgg taatcagcca
    46321 tgattaacgt accttcagcg ttgccagacc aatcaggacc agcatcagcg aaataatcca
    46381 gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt gatgaatcgg
    46441 tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca ggatgacaga
    46501 aagcgtttct accacgaaca cgccccccat aatcaccagc aggaattcct gacgtagcag
    46561 tacggcgata atgcctaacg caccacctaa cgccagcgaa ccgacatcgc ccataaagac
    46621 ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta tcgcggtaca
    46681 gacaataacc agttccccgg cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt
    46741 catgttgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat
    46801 aattgccaga ccgtcaagac catcggtcag gtttaccgcg ttgccagtac ccacaatgac
    46861 gaagtaagcc agcagaatgt agaacagccc cagttgcggc atcacatctt taaagaatgg
    46921 gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc
    46981 cagcgcaatg accgacatcc agaaatattt ccaacgagcg atcaaccctt tggtgtcttt
    47041 acgcaccact ttgcgataat catcaacaaa gccaataaca ccgtaaccta ccagcaccac
    47101 caacacgcac cagacgtacg gattggacgg gtaagcccac agcagcacgg agatcacaat
    47161 cgctgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga agtgtgattc
    47221 aggaccgtcg ttacgcacta cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg
    47281 cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt
    47341 cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg ccagccaaac
    47401 taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcagc actacgtgaa
    47461 cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag acgcgtaatt
    47521 aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat agcatggctt
    47581 tgtttaccca tgcttaacac gcggtcaata ccagcagctt ttgccgcctc acccacctgt
    47641 acatggcagg cttcgctttc agcgcccagt tccgccatat cgcccaccac cagcacgcgg
    47701 tagcccggca tttcagccag tacctgaaca gctgcggtca ttgaaccgac attggcgttg
    47761 taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag acggccagga
    47821 acagctttca gatttgccag ccccgctttg atagcatcaa gcgttgcgcc cacggacatg
    47881 gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa cggcagcaga
    47941 acatcgacgc taccggttgg ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg
    48001 atattggtgg cggtgaaatc gctgttggcg gcattgggtg agaaacgcca cactttgcgt
    48061 gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat aatggcgata
    48121 ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc cgcaagcgag
    48181 ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc
    48241 agactcacag tccaggcaat ctcgccctga tggttcgcgc caagttcaat aactgcgtaa
    48301 tcgtattccg gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt gttgagattg
    48361 cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg
    48421 gaggttttgc cggaggaccc cgtcagagca accacgcgcg ccggaacttg ctggcgaacc
    48481 catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg cggcaggtcg
    48541 atgtccagcg gacggctaac cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca
    48601 aaatcgtggg catcaaaacg ttcacctttc agggcaacaa acaggcagcc cggcgtcagt
    48661 tttcgggtat cagtggttac agcatcaagg gtgatatctg caccttgcag ttcaccgttg
    48721 agaatatcgg taagttggct aagggttacg ctaatcatgc aatccccccc agcagacgcg
    48781 ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg taatcctcat
    48841 ggcctttgcc cgcgaccagt accacatcat tctctttagc ctgcataacg gcgcaagtca
    48901 ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt cccgccagaa
    48961 tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca
    49021 cgtcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta tcgcgatcgc
    49081 caccacagcc aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg
    49141 ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg
    49201 cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat
    49261 cagccagtgg atagcccagc gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt
    49321 taaaagcgcc catcagatgg ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa
    49381 tggtcgcgcc gctgtcgtga tagttcactt cggtcgcttt caaccagcgt ccgtgacagt
    49441 tcggattgat atgatcttcc attgataccg caaccgcgtc cggcagtttt gccagccagc
    49501 ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa
    49561 gcagccattt cgcagcttcg tagtgttcca tatcaccatg ataatcaagg tgatcgcggc
    49621 ttaagttggt aaagaccgac gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc
    49681 cgtgggagga aacttccatt gcgcaaaaag tcgcgccctg atccaccagc cccgccagct
    49741 catgctgaac atcaactgcc gaaccggtag tattttctgt cgggatcact ttccccagca
    49801 gaccgttacc aacggtgccc attaccgcgc tggtttcgcc aagcagttgg ctccactgcg
    49861 ccagaagctg ggtcgtcgtg gttttgccgt tggtgcccgt tacgcctacg agacgtaaat
    49921 tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg ttgagctggc
    49981 tgagatagat gaccggtacg ccgtgcattt cacggatttc accgtcggtc gcctcatctt
    50041 tcgcctctgc aataatggca gccacacctt gcgctatcgc ctgcgggata tatcgacgcc
    50101 cgtccgcctg atgacctact acagctacaa agagatcgcc cgccgcagcc acacggctgt
    50161 cgagtgtcat ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt
    50221 cgcgcaaatt acgatctgcc acctgtcccc tcgccttgat taatcacaaa ttcattttta
    50281 tcgcccgttg tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc gcccatgatg
    50341 gcaccaaaga ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg cggatcgttg
    50401 ataacaacaa ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc ggtataagca
    50461 atatatttat tgatgtagcg accgtccggc ccgacctttt tcgcggtacc ggttttaatg
    50521 gcgatacgat agcctttaat cgccgccttc acgccgccgc cgcctggtag cgccacgctt
    50581 tccatcatat gcaccacggt gcgaacaatg gattccggga agacacgttc accgggaacc
    50641 ggggggtcaa ctttggtaat cgacagtggg cgataaatgc cgtagctgcc gatagttgcg
    50701 tagactcgcg ctaactgtaa cggtgttacc attagcccgt agccgaaaga gaaggtggcc
    50761 ctctctatgt cagaccaccg ttgtttttga ggatataagc cactgcgttc tccgaccaac
    50821 cccaaattgg tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa cgctgaggac
    50881 ggcatcgtta acgccagctt ggaaacaccg acgttactcg acttctgtaa taccccggtc
    50941 agggttaatt cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat tcgataagga
    51001 atggtattga gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt catcaccacc
    51061 atcggtttaa ccgttgagcc cggttcaaac acgtcggtga tggtacggtt acgcatcgcc
    51121 tctttcggcg tgccgctcag attgttaggg ttgtatgacg ggctgttagc catcgccagc
    51181 acttcacctg tgttgacatc caccagcacg gcgctaccag attcagcctt gttaaaggcc
    51241 accgcgttgt tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat actcagcgcc
    51301 aggttgtgcg ctgcctggct gtcagtagaa gaaatatctt caattacgcg accatagcgg
    51361 tctttacgca caatgcgctc acccggctgc ccggtaagcc atttatcgaa actcttctca
    51421 acgccctcaa tcccttgact atcgacgtta gtaaagccga tgaggtgagc agtcacttcg
    51481 ccggacggat agtaacggcg agactcttca cgcagatgaa tccccggcag tttcagtttt
    51541 ttgatgtagt ccgccatgtc agagtttacc tgacgcgcca gataaataaa gcgccctttc
    51601 gggttggcgt taatgcgggc agaaagctga tccagcggaa tattgagcgc gttagccagc
    51661 gccttccagc ggtcaccgac gctgataccg ccagcgtcat gcacttcttt cgggtcagcc
    51721 caaatcgctt ttaccggcac gctcaccgct aacgggcgac cagaacggtc agtaatcatg
    51781 ccgcgggagg tggaaacttg ctgaacgcga agagaacgca tgtcgccctc tttcaccagc
    51841 atatccgggg agataacttg taaccacgct acgcgtccga gcagaaaagc cagcgccagg
    51901 agaatacagc cgcataacaa cgcaaaacgc caactgataa agttggcatg ttcttcctga
    51961 cgttttggtt tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc
    52021 actacgatat tttcttgtga cggatcaaca tgctgcattt gcagcttttc cgtggcgatc
    52081 ctttccaccc ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt gcgccattca
    52141 atgtctaaag catctcgctc cagcaccagt tgttcgcgct gagcggtcag taaacgggta
    52201 tggtgcgccg tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt
    52261 ggcagcttcc caaatcgcaa aagatcgtca ccgataacac caggcaatgc atggcgctcg
    52321 tggcttccca tcgatccttt aactttgctt agagcttctg tcactctgct gatcatgcgt
    52381 tcgtcctctc tgcaatacgc agaactgaac tacgggcacg agggttctca gccacctctt
    52441 cttcgcccgg cattaacttg cctagtgctc gcagctgacg gccacccagt tttttgagct
    52501 gctcttcagt catcggtaac cctgccggaa cttgcggacc gcggctgttt tcacgcataa
    52561 aacgtttcac aatacggtct tccagcgagt ggaagctgat gatcgaaagc cgcccacccg
    52621 gggccagcac gttgagcgag ctttttagcg cctgctctat ctcctccagt tcactgttta
    52681 cccaaatgcg caccgcctgg aaggtacggg tcgcgggatg tttaaactta tctttcaccg
    52741 gcgttgcagc agccacgact tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt
    52801 tacgctcgac aatggcgcgg gcaatgcgtt tggcaaaatg ctcttcacca taggttttca
    52861 atacccaggc gatatcggct tcttctgcgg tttgtagcca ttcagcggct gactgcccac
    52921 gggttgggtc catacgcatg tccagcggac catcgcgcat aaaggaaaag ccgcgttcag
    52981 catcatcaag ttgcggtgaa gagacgccaa gatcgaggag aatgccgtcg atcttgccaa
    53041 taagatcgcg ctcggcaacg tattcgccca gcgcggagaa aggtccgtgg atgatggaga
    53101 agcgcggatc atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca
    53161 gcaaacgccc ctcttcgcca agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa
    53221 aagtcccatc aatgtagatg ccatcaggac ggatattgag gccattaacg gcttcatcca
    53281 gcagcaccgt agtatgttta tagttttcca tcattttata gagacaagtc ctgcagtcgc
    53341 tccgataagt ctccggtagc caactgctct gcgtcgatat cttccttgac ctgttgatgc
    53401 caggttgttt catcccacag ctcaaacttg ttgaactgtc caaccagcat cacttctttt
    53461 gtcagcccgg catgttgccg cagtactggc gcgattaaca atcgacctgc gccatccatc
    53521 tgaca
//
LOCUS       DAJTRB010000016        52726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTRB010000016 DAJTRB010000000
VERSION     DAJTRB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.016"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(11..322)
                     /locus_tag="OO330_001042"
     CDS             complement(11..322)
                     /locus_tag="OO330_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5881995.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO330_001043"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO330_001043"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO330_001044"
     CDS             701..1054
                     /locus_tag="OO330_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5881996.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO330_001045"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO330_001045"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5881997.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO330_001046"
     CDS             complement(2810..3280)
                     /locus_tag="OO330_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5881998.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO330_001047"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO330_001047"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5881999.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO330_001048"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO330_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5882000.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO330_001049"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO330_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5882001.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OO330_001050"
     CDS             complement(5283..8432)
                     /locus_tag="OO330_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5882002.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO330_001051"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO330_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5882003.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OO330_001052"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OO330_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5882004.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OO330_001053"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OO330_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5882005.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNVMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO330_001054"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO330_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5882006.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO330_001055"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO330_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5882007.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OO330_001056"
     CDS             14911..15288
                     /locus_tag="OO330_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5882008.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OO330_001057"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OO330_001057"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5882009.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OO330_001058"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OO330_001058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5882010.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OO330_001058"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OO330_001059"
     CDS             18099..18428
                     /locus_tag="OO330_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5882011.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OO330_001060"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OO330_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5882012.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OO330_001061"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OO330_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5882013.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OO330_001062"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OO330_001062"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5882014.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OO330_001063"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OO330_001063"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5882015.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO330_001064"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO330_001064"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5882016.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OO330_001065"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OO330_001065"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5882017.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OO330_001066"
     CDS             complement(25481..27157)
                     /locus_tag="OO330_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5882018.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO330_001067"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO330_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5882019.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLMAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OO330_001068"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OO330_001068"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5882020.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO330_001069"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO330_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5882021.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OO330_001070"
     CDS             complement(31205..31999)
                     /locus_tag="OO330_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5882022.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OO330_001071"
     CDS             32137..32478
                     /locus_tag="OO330_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5882023.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO330_001072"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO330_001072"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5882024.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSSSPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OO330_001073"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OO330_001073"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5882025.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OO330_001074"
     CDS             36394..37686
                     /locus_tag="OO330_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5882026.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALGLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="OO330_001075"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="OO330_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5882027.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="OO330_001076"
     CDS             complement(38219..38677)
                     /locus_tag="OO330_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5882028.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OO330_001077"
     CDS             complement(38674..39591)
                     /locus_tag="OO330_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5882029.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OO330_001078"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OO330_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5882030.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OO330_001079"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OO330_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5882031.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO330_001080"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO330_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5882032.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO330_001081"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO330_001081"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5882033.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO330_001082"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO330_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OO330_001083"
     CDS             43550..44236
                     /locus_tag="OO330_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5882034.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46642
                     /locus_tag="OO330_001084"
                     /pseudo
     CDS             44233..46642
                     /locus_tag="OO330_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(46876..47970)
                     /gene="mnmH"
                     /locus_tag="OO330_001085"
     CDS             complement(46876..47970)
                     /gene="mnmH"
                     /locus_tag="OO330_001085"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5882035.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48039..48965)
                     /gene="allS"
                     /locus_tag="OO330_001086"
     CDS             complement(48039..48965)
                     /gene="allS"
                     /locus_tag="OO330_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5882036.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49195..49677
                     /gene="allA"
                     /locus_tag="OO330_001087"
     CDS             49195..49677
                     /gene="allA"
                     /locus_tag="OO330_001087"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5882037.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            49755..50570
                     /gene="allR"
                     /locus_tag="OO330_001088"
     CDS             49755..50570
                     /gene="allR"
                     /locus_tag="OO330_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5882038.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            50660..52441
                     /gene="gcl"
                     /locus_tag="OO330_001089"
     CDS             50660..52441
                     /gene="gcl"
                     /locus_tag="OO330_001089"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5882039.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52454..>52726
                     /locus_tag="OO330_001090"
     CDS             52454..>52726
                     /locus_tag="OO330_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5882040.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12690 a        13575 c        13919 g        12542 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgtccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgtgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggatactttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ttggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaatgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatccct aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccatcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggaactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct gggtttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagta caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtag cgatgaatca ttactgtcgc agtcgctacg atctggtggc gatcctcaaa
    45061 acgctggggg cagggcgagc gcaactgcgt aagctaatcg tcggtcagtg gttgatggtg
    45121 ctgacgcttt cagccgttac cggtggggcc ataggcctgt tgttcgaaaa cgtgttgatg
    45181 gtgctgctca agcccgttct gcctgctgca ctaccgccag ccagcctctg gccgtggctg
    45241 tgggcgcttg gcaccatgac ggtcatctcg ctgctggtgg ggctacgacc ataccgcttg
    45301 ttgctggcaa cgcagccttt acgcgtatta cgtaatgatg tggtcgcgaa cgtctggccg
    45361 ctgaagtttt atctgccgat tgtcagtgtg gtggttgtgc tgctgctcgc cggattaatg
    45421 ggtggcagca tgctgctttg ggcggtgctg gcgggcgcgg tagtactggc tttgctgtgc
    45481 ggtgtgctgg gctggatgct gctgaatgta cttcgccgca tgacgctgaa atcgctgcct
    45541 ctgcgcctgg cggttagccg cctgttacgt cagccgtggt caacgttaag ccagctttcg
    45601 gcattttcgc tctcctttat gctgctggca ctgctgctgg tgctgcgtgg cgatctgctc
    45661 gaccgctggc agcagcagct accgccagaa agcccgaact actttttaat taacatcgcc
    45721 acagaacagg tagcaccgct aaaagcgttc ctcgcggaac atcatatagt cccggaatca
    45781 ttttatccgg tagtgcgggc gcggctgacg gcgattaatg ataagccgac agaaggtaat
    45841 gaagatgagg cgcttaatcg cgaactcaat cttacctggc aaaatacgcg gcccgatcat
    45901 aatccgattg tcgccggtaa ctggccgcca aaagccgatg aagtgtcgat ggaagagggg
    45961 ctggcgaaac gcttaaacgt tgccctcggt gataccgtga cttttatggg cgatacccag
    46021 gagttccgcg ctaaagtgac cagcctgcgc aaagtggact gggaaagtct gcggcctaat
    46081 ttctatttta ttttccctga aggggcatta gacgggcaac cgcagagctg gcttaccagt
    46141 ttccgctggg agaatggcaa cggcatgttg acgcaactca accgccagtt cccgaccatt
    46201 agcctgttag acattggcgc gattttaaaa caggtcggtc aggtgctgga gcaggtaagt
    46261 cgggcgctgg aggtgatggt ggtgctggtc accgcctgcg gtatgttgct gttgctggca
    46321 caggtgcagg tgggaatgcg ccagcgtcat caggagctgg tggtatggcg cacactcggt
    46381 gcggggaaaa aactgctgcg cacgacattg tggtgtgatg ttcgccatgc ttgggtttgt
    46441 ttccggcctg gtggccgcaa ttggtgcgga aacggcactg gcagtgttgc aggcgaaagt
    46501 gtttgatttc ccgtgggagc cagactggcg attgtggatt gttctgccgt gcagcggtgc
    46561 gctgctgctg tcgcttttcg gcggctggct gggtgcgcga ctggttaagg gtaaggcgct
    46621 gttcaggcag tttgtggggt gatgacaggt gttagattgc ctgatgcgct acgcttatca
    46681 ggcctacgaa gagcgtgcaa tatattgatt ttgcaagatt gtgtaggccg gataaggcgt
    46741 ttacgccgca tctggcatga acagtgtgca ttttggcaat aaatatgaaa ccgactgtca
    46801 tgtcggtttt ttatatctgt attctggtgc aaccattttg ttctcacgac aatcgccgtt
    46861 tcagaatacc gcccatcact gcgccttaac ccataccgcc acttccgccc actcaccgcg
    46921 aaagacaact ttttccgctt ttttctcaag ctgatagcga tacattgggt cgtaatattc
    46981 tttaagtaac ggcaccagcc aggccagatg accgtcggtg ctgccggtgg tgagttgcgt
    47041 tgtcagcgcc gtatccagct gggcagcgag ttcgttatag cgctgtaacc ccagccgacg
    47101 cttaatcgcc gaaagtccgt gatgcaggta ttcgcaatat tcctgccagc cctgttcgtc
    47161 gccataagcg tgggtaaaat catgatgcat acgcaagaaa tactcttcat tcaggcgttc
    47221 aagacggatc tcaaacggat cttctaccac cgcaatcgcc gcctgagtca ttcgctcgcg
    47281 cagacattcc ggcaggtgat tcgaaccgat catccggctt tcatcttcca gcacccacag
    47341 gcgcaaatcc tgacgggcgt cggtttttag catttcggca gccagcaggt tttcaaaact
    47401 cgcctggcta agttgtggtt gtaacgtgcg accaaacgcc gaaccgcgat gacgcgccag
    47461 cccttccaga tcaacaccgt ttggctgttg ttgcactaat agcgttttac cgctgccggt
    47521 acaaccgcca atcagcacta tcggtttttg tgccagttca atggtcgcct gaatcgcggt
    47581 ctggcgcagt gccttataac cgccttccac cagcggataa tcaatccccg cttcatgcag
    47641 ccagcgttgc acaatatgtg aacgttgacc gccacgggcg cagcagagaa taccttgcgg
    47701 attttgcagg cacgctgccc gccaggcgta catacgctgc tgacgaattt cacccgccac
    47761 cagtttatgc cccagcgcca gcgctgcgtc tgagccttgc tgtttatagc aggtgccaac
    47821 ggaggcgcgt tcatcgttat ccattaacgg cagattgata gcggcgggca ttgcgccttg
    47881 ctcaaactcg ataggggcgc gaacatcaat aatgggcgta tcagcaatca gcagggcacg
    47941 atagtcctgt tccgtgtgtc tctcttgcat agttaaaagt gaacctcaaa tcagcttgcg
    48001 cgctatttta cgcgccaacg cgcaaggaaa cttgattttt aactgcgtgg ggtgccgaaa
    48061 atttctaaaa atccgctgat ttccggcctg tgttgggtaa acaaggtcac aatatcttct
    48121 accgctttgc agctgccaaa tttgcgccat gccagactca atggcgaagg agggcgcatc
    48181 gttgggatta cccggctgac cagttgttga ttatcgatca ttgactggca aagtgatttt
    48241 ggcaaaaaac caatgccaac acccgccaga tgggcggcga ttttcgtttc catatcggga
    48301 acaataatct ctttttgccc cggcaatcgc caggcgacgc gtttggttaa ggtgccggcg
    48361 ctgtcttcaa tattgaccgc cggaaagcga cgcaactgcg cttctgttag cggctcttca
    48421 acgttcgcca gcggatgatc cgccgccatg acaaatcgcc attgcaccga tcctaaggga
    48481 tcaagactaa aggtatttgc cagcgcctca gttcccgtga ccccgatagc cagcgaaaaa
    48541 ccttcgtaca atagcgagtc ccagacgccc atatagattt gtcgggagat gtgaaactgg
    48601 gtaaaggggt aacgctcatt cagccacgcc agcaactggg cgacggcctg ggggttatag
    48661 agcaggttgt tgatgacaat attcacctgg cgttccacgc catcattcac ctgttgcagc
    48721 tcgctgggca tactttccag ccagctcagc cagtctctgg cctgggaaag tagatgctcg
    48781 ccagccgctg tcaacgttac gctacgagtc gtacggaaaa acagcgctac tccggtattc
    48841 tcttccagaa gtttaatgcg ataactgatc gtcgccgtgg ttttacataa tcgttctgcc
    48901 gcttttgaaa aacttcctgt ttcagcaacc gcaatgaaag tccgcaaggt ttctggatcg
    48961 aacatcttca ggtatcccct tttaaatccg caagttgcgt gattttctta tcctctgatt
    49021 tatcagtatt tttacatgat aatcctgttc aatttgtgga ctaaatctag ttttggaaaa
    49081 acattccaac ttttgtattg atgctgttct cttaaggttt tagattgcct gttattgaaa
    49141 ccaagctgac cggtcggcgg tggttgaacg gaattatgtt acaaggacaa aaagatgaaa
    49201 cttcaggtat taccgttaag tcaggaagcc tttagtgctt atggcgacgt aatcgaaacg
    49261 cagcaacggg attttttcca tattaacaat ggcctggtgg agcgttacca cgatttggcg
    49321 ctggttgaga ttcttgagca agaccgtacg cttatcagca ttaaccgcgc gcaaccggcg
    49381 aatctgccgc tgaccattca cgaactcgaa cgtcatccgc tgggcactca ggcctttatc
    49441 ccaatgaaag gtgaagtgtt tgtggtggtc gtggcgttag gcgacgacaa accagacctg
    49501 tcaacgctgc gggcgtttat caccaacggt gaacaggggg tgaattacca tcgtaacgtc
    49561 tggcatcacc cacttttcgc ctggcagcgc gtcaccgatt ttctgaccat cgatcgcggc
    49621 ggcagtgaca actgtgatgt tgaaagtatt cctgaacagg aactctgttt tgcgtgacgc
    49681 ctgcaaccga cttgcataag ataaactaat tgttcattgt ttatgctcac ttgtaggtcg
    49741 gagttaacgt aggtatgacg gaagttagac ggcgcggcag gccaggacag gcggagcctg
    49801 tggcacagaa gggcgcacag gcgttagagc ggggaattgc gattctgcaa tatctggaaa
    49861 aaagtggggg aagttcgtcg gttagcgata tttctctcaa tctggatttg ccgctctcca
    49921 cgacctttcg cttgctgaag gttttacaag cagcggattt tgtctatcag gacagtcaat
    49981 taggctggtg gcatatagga ttaggtgtct ttaacgtcgg tgcggcgtac atccataacc
    50041 gcgatgtcct ctccgtcgcc gggccgttta tgcgccgcct gatgttactt tccggcgaaa
    50101 cggtcaatgt cgcgatccgt aacggcaatg aagcggtatt aattggtcag ttagagtgta
    50161 aatcgatggt caggatgtgt gcgccactgg gcagtcgtct gccgctgcat gcttccggtg
    50221 cgggcaaagc gctgctttat ccgctggcgg aagaggagtt gatgagcatc attctgcaaa
    50281 ccggtttgca gcagtttacg ccaactacgc ttgtggatat gcccaccttg ctgaaggacc
    50341 tggaacaagc gcgtgaactg ggctataccg tagataaaga agagcatgtt gtaggtctga
    50401 attgcatagc ttcagcaatt tacgatgatg tcggtagagt tgttgccgct atctccatct
    50461 ccgggccttc atcaagactg acagaagatc gttttgtcag tcagggtgag ctggtcagag
    50521 acaccgcccg cgatatcagc acggcgttgg gactgaaagc acatccatag tgtctgtcgc
    50581 atcccgctct gcggagcggg tttttttgac aaaatttgaa agttggaaaa tttttccaat
    50641 taatagaggt aggaataaaa tggcaaaaat gagagccgtt gacgcggcaa tgtatgtgct
    50701 ggagaaagaa ggtatcacca ccgccttcgg cgttccggga gctgcaatta atccgttcta
    50761 ctcagcgatg cgtaagcacg gcggtattcg tcacattctg gcgcgtcatg tggaaggtgt
    50821 ttcgcacatg gcggaaggtt atacccgcgc aacggcaggg aatatcggcg tatgtctggg
    50881 gacttccggt cctgcgggca cggacatgat caccgcgctc tattccgctt ctgctgattc
    50941 cattcctatt ctgtgcatta ccggccaggc accgcgcgcc cgtctgcata aagaagattt
    51001 tcaggccgta gatattgaag caattgctaa accggtcagc aaaatggcgg ttacagttcg
    51061 tgaagcggcg ctggtgcctc gcgtgctgca acaggcattt cacctgatgc gttctggtcg
    51121 tccgggtccg gtactggtgg atttaccgtt cgacgttcag gttgcggaaa tcgagtttga
    51181 tcctgacatg tacgaaccgc tgccggtcta caaacctgct gccagccgta tgcagatcga
    51241 aaaagctgta gaaatgttaa tccaggccga acgtccggtg attgttgccg ggggcggggt
    51301 aatcaatgct gacgcagctg cactgttaca acagtttgct gaactgacca gcgttccggt
    51361 gatcccaacg ctgatgggct ggggctgtat cccggacgat catgaactga tggccgggat
    51421 ggtcggtctg caaaccgcgc atcgttacgg taacgcaacg ttgctggcgt ccgacatggt
    51481 gtttggtatc ggtaaccgtt ttgctaaccg tcataccggt tcggtagaga aatacaccga
    51541 agggcgcaaa atcgttcata ttgatattga gccgacgcaa attggtcgcg tgctgtgtcc
    51601 ggatctcggc attgtctctg atgctaaagc ggcgctgaca ctgctggttg aagtggcgca
    51661 ggagatgcaa aaagcgggtc gtctgccgtg tcgtaaagaa tgggtcgccg actgccagca
    51721 gcgtaaacgc actttgctgc gcaaaaccca cttcgacaac gtgccggtga aaccgcagcg
    51781 cgtgtatgaa gagatgaaca aagcctttgg tcgcgatgtt tgttatgtca ccactattgg
    51841 tctgtcacaa atcgctgcgg cacaaatgct gcatgtcttt aaagaccgcc actggatcaa
    51901 ctgtggtcag gctggtccgt taggctggac gattccggcg gcgctggggg tttgtgccgc
    51961 tgatccgaaa cgcaatgtgg tggcgatttc tggcgacttt gacttccagt tcctgattga
    52021 agagttagcc gttggcgcgc agttcaacat tccgtacatc catgtgctgg tgaataacgc
    52081 ctatctcggc ctgattcgtc agtcacaacg cgcttttgac atggactact gcgtgcaact
    52141 cgctttcgag aatatcaact ccagtgaagt gaatggctac ggtgttgacc acgtaaaagt
    52201 agcggaaggt ttaggttgta aagctattcg ggtcttcaaa ccggaagata ttgcgccagc
    52261 ctttgaacag gcgaaagcct taatggcgca atatcgggta ccggtagtcg tggaagttat
    52321 tctcgagcgt gtgaccaata tttcgatggg cagcgaactg gataacgtca tggaatttga
    52381 agatatcgcc gataacgcag cggatgcacc gactgaaacc tgcttcatgc actatgaata
    52441 agggagataa atgatgttac gtttctctgc taatttatcg atgttatttg gtgaatatga
    52501 ttttctcgcc cgttttgaga aagctgcgca gtgtggtttt cgcggcgttg aatttatgtt
    52561 tccttatgac tacgacattg aagaattaaa acaggtactg gcgagtaata aactcgaaca
    52621 tacgctgcac aatttaccgg caggtgactg ggcggcgggc gagcgcggta ttgcctgtat
    52681 tcctggccgt gaagaagagt ttcgggatgg cgtagcagca gcgatt
//
LOCUS       DAJTRB010000017        52559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTRB010000017 DAJTRB010000000
VERSION     DAJTRB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.017"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            84..1400
                     /gene="fucP"
                     /locus_tag="OO330_001091"
     CDS             84..1400
                     /gene="fucP"
                     /locus_tag="OO330_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5882041.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1433..3208
                     /gene="fucI"
                     /locus_tag="OO330_001092"
     CDS             1433..3208
                     /gene="fucI"
                     /locus_tag="OO330_001092"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5882042.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3317..4133
                     /gene="fucK"
                     /locus_tag="OO330_001093"
                     /pseudo
     CDS             3317..4133
                     /gene="fucK"
                     /locus_tag="OO330_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            4163..4327
                     /gene="fucU"
                     /locus_tag="OO330_001094"
                     /pseudo
     CDS             4163..4327
                     /gene="fucU"
                     /locus_tag="OO330_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="catalyzes the interconversion of alpha-L-fucose to
                     beta-L-fucose; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
     gene            4385..5116
                     /gene="fucR"
                     /locus_tag="OO330_001095"
     CDS             4385..5116
                     /gene="fucR"
                     /locus_tag="OO330_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5882043.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(5160..6260)
                     /gene="rlmM"
                     /locus_tag="OO330_001096"
     CDS             complement(5160..6260)
                     /gene="rlmM"
                     /locus_tag="OO330_001096"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5882044.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(6253..6648)
                     /locus_tag="OO330_001097"
     CDS             complement(6253..6648)
                     /locus_tag="OO330_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5882045.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(6667..7584)
                     /gene="gcvA"
                     /locus_tag="OO330_001098"
     CDS             complement(6667..7584)
                     /gene="gcvA"
                     /locus_tag="OO330_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5882046.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(7935..8162)
                     /locus_tag="OO330_001099"
     CDS             complement(7935..8162)
                     /locus_tag="OO330_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5882047.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            8354..9559
                     /gene="csdA"
                     /locus_tag="OO330_001100"
     CDS             8354..9559
                     /gene="csdA"
                     /locus_tag="OO330_001100"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5882048.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            9559..10002
                     /gene="csdE"
                     /locus_tag="OO330_001101"
     CDS             9559..10002
                     /gene="csdE"
                     /locus_tag="OO330_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5882049.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(10053..10859)
                     /gene="tcdA"
                     /locus_tag="OO330_001102"
     CDS             complement(10053..10859)
                     /gene="tcdA"
                     /locus_tag="OO330_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5882050.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(11098..12195)
                     /gene="mltA"
                     /locus_tag="OO330_001103"
     CDS             complement(11098..12195)
                     /gene="mltA"
                     /locus_tag="OO330_001103"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5882051.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            12404..12480
                     /locus_tag="OO330_001104"
     tRNA            12404..12480
                     /locus_tag="OO330_001104"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12438..12440,aa:Met,seq:cat)
     gene            12514..12590
                     /locus_tag="OO330_001105"
     tRNA            12514..12590
                     /locus_tag="OO330_001105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12548..12550,aa:Met,seq:cat)
     gene            complement(12664..13917)
                     /gene="amiC"
                     /locus_tag="OO330_001106"
     CDS             complement(12664..13917)
                     /gene="amiC"
                     /locus_tag="OO330_001106"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5882052.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            14149..15480
                     /gene="argA"
                     /locus_tag="OO330_001107"
     CDS             14149..15480
                     /gene="argA"
                     /locus_tag="OO330_001107"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5882053.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(15542..17368)
                     /gene="recD"
                     /locus_tag="OO330_001108"
     CDS             complement(15542..17368)
                     /gene="recD"
                     /locus_tag="OO330_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5882054.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(17368..20910)
                     /gene="recB"
                     /locus_tag="OO330_001109"
     CDS             complement(17368..20910)
                     /gene="recB"
                     /locus_tag="OO330_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5882055.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDTVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(20903..23791)
                     /gene="ptrA"
                     /locus_tag="OO330_001110"
     CDS             complement(20903..23791)
                     /gene="ptrA"
                     /locus_tag="OO330_001110"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5882056.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMM
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(23967..27335)
                     /gene="recC"
                     /locus_tag="OO330_001111"
     CDS             complement(23967..27335)
                     /gene="recC"
                     /locus_tag="OO330_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5882057.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(27348..27680)
                     /locus_tag="OO330_001112"
     CDS             complement(27348..27680)
                     /locus_tag="OO330_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5882058.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(27665..28072)
                     /locus_tag="OO330_001113"
     CDS             complement(27665..28072)
                     /locus_tag="OO330_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5882059.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(28069..28632)
                     /locus_tag="OO330_001114"
     CDS             complement(28069..28632)
                     /locus_tag="OO330_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5882060.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNR"
     gene            complement(28623..29093)
                     /locus_tag="OO330_001115"
                     /pseudo
     CDS             complement(28623..29093)
                     /locus_tag="OO330_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(29277..30071)
                     /gene="thyA"
                     /locus_tag="OO330_001116"
     CDS             complement(29277..30071)
                     /gene="thyA"
                     /locus_tag="OO330_001116"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5882061.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(30078..30953)
                     /gene="lgt"
                     /locus_tag="OO330_001117"
     CDS             complement(30078..30953)
                     /gene="lgt"
                     /locus_tag="OO330_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5882062.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(31104..33350)
                     /gene="ptsP"
                     /locus_tag="OO330_001118"
     CDS             complement(31104..33350)
                     /gene="ptsP"
                     /locus_tag="OO330_001118"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5882063.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKTRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(33363..33893)
                     /gene="rppH"
                     /locus_tag="OO330_001119"
     CDS             complement(33363..33893)
                     /gene="rppH"
                     /locus_tag="OO330_001119"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5882064.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            34578..35267
                     /gene="mutH"
                     /locus_tag="OO330_001120"
     CDS             34578..35267
                     /gene="mutH"
                     /locus_tag="OO330_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5882065.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            35336..36049
                     /locus_tag="OO330_001121"
     CDS             35336..36049
                     /locus_tag="OO330_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5882066.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            36187..36405
                     /gene="ygdR"
                     /locus_tag="OO330_001122"
     CDS             36187..36405
                     /gene="ygdR"
                     /locus_tag="OO330_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5882067.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            36513..37553
                     /locus_tag="OO330_001123"
     CDS             36513..37553
                     /locus_tag="OO330_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5882068.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(37586..38776)
                     /gene="lplT"
                     /locus_tag="OO330_001124"
     CDS             complement(37586..38776)
                     /gene="lplT"
                     /locus_tag="OO330_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5882069.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(38769..40928)
                     /gene="aas"
                     /locus_tag="OO330_001125"
     CDS             complement(38769..40928)
                     /gene="aas"
                     /locus_tag="OO330_001125"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5882070.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            41514..42545
                     /gene="galR"
                     /locus_tag="OO330_001126"
     CDS             41514..42545
                     /gene="galR"
                     /locus_tag="OO330_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5882071.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(42552..43814)
                     /gene="lysA"
                     /locus_tag="OO330_001127"
     CDS             complement(42552..43814)
                     /gene="lysA"
                     /locus_tag="OO330_001127"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5882072.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            43936..44871
                     /gene="lysR"
                     /locus_tag="OO330_001128"
     CDS             43936..44871
                     /gene="lysR"
                     /locus_tag="OO330_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5882073.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(44858..45550)
                     /gene="ygeA"
                     /locus_tag="OO330_001129"
     CDS             complement(44858..45550)
                     /gene="ygeA"
                     /locus_tag="OO330_001129"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5882074.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(45679..47097)
                     /gene="araE"
                     /locus_tag="OO330_001130"
     CDS             complement(45679..47097)
                     /gene="araE"
                     /locus_tag="OO330_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5882075.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(47412..48173)
                     /gene="kduD"
                     /locus_tag="OO330_001131"
     CDS             complement(47412..48173)
                     /gene="kduD"
                     /locus_tag="OO330_001131"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5882076.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(48203..49039)
                     /gene="kduI"
                     /locus_tag="OO330_001132"
     CDS             complement(48203..49039)
                     /gene="kduI"
                     /locus_tag="OO330_001132"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5882077.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(49326..50507)
                     /locus_tag="OO330_001133"
     CDS             complement(49326..50507)
                     /locus_tag="OO330_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5882078.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            50762..51991
                     /locus_tag="OO330_001134"
                     /pseudo
     CDS             50762..51991
                     /locus_tag="OO330_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        13024 a        14177 c        12793 g        12565 t
ORIGIN      
        1 atatgaagcc cgaactcgct ggttttgcac ttttgaaaac ataaccgatt acgtgcttaa
       61 gcttctgaac ctaagaggat gctatgggaa acacatcaat acaaacgcag agttaccgtg
      121 cggtagataa agatgcaggg caaagcagaa gttacattat tccattcgcg ctgctgtgct
      181 cactgttttt tctttgggcg gtagccaata accttaacga cattttatta cctcaattcc
      241 agcaggcttt tacgctgaca aatttccagg ctggcctgat ccaatcggcc ttttactttg
      301 gttatttcat tatcccaatc cctgctggga tattgatgaa aaaactcagt tataaagcag
      361 ggattattac cggattattt ttatatgcct tgggcgctgc attattctgg cccgctgcag
      421 aaataatgaa ctacacctta tttttagttg gcctatttat tattgcagcc ggattaggtt
      481 gtctggaaac tgccgcaaac ccttttgtta cggtattagg gccggaaagc agtagtcact
      541 tccgcttaaa tcttgcgcaa acatttaact cgtttggcgc aattatcgcg gttgtctttg
      601 ggcaaagtct tattttgtct aacgtgccac atcaatcgca agacgttctc gataaaatgt
      661 ctccagagca attgagtgcg tataaacaca gcctggtatt atcggtacag acaccttata
      721 tgatcatcgt ggctatcgtg ttactggtcg ccctgctgat catgctgacg aaattcccgg
      781 cattgcagag tgataatcac agtgacgcca aacaaggatc gttctccgca tcgctttctc
      841 gtctggcgcg tattcgtcac tggcgctggg cggtattagc gcaattctgc tatgtcggcg
      901 cacaaacggc ctgctggagc tatttgattc gctacgctgt agaagaaatt ccaggtatga
      961 ctgcaggctt tgccgctaac tatttaaccg gaaccatggt gtgcttcttt attggtcgtt
     1021 tcaccggtac ctggctcatc agtcgcttcg caccacacaa agtcctggcc gcctacgcat
     1081 taatcgctat ggcactgtgc ctgatctcag ccttcgctgg cggtcatgtg ggcttaatag
     1141 ccctgacttt atgcagcgcc tttatgtcga ttcagtaccc aacaatcttc tcgctgggca
     1201 ttaagaatct cggccaggac accaaatacg gttcgtcctt catcgttatg accattattg
     1261 gcggcggtat tgtcactccg gtcatgggtt ttgtcagtga cgcggcgggc aacatcccca
     1321 ctgctgaact gatccccgca ctctgcttcg cggtcatctt tatctttgcc cgtttccgtt
     1381 ctcaaacggc aactaactga acatattttc cgaataaagt gaggaatctg taatgaaaaa
     1441 aatcagctta ccgaaaattg gtatccgccc ggttattgac ggtcgtcgca tgggtgttcg
     1501 tgagtcgctt gaagaacaaa caatgaatat ggcgaaagct acggccgcac tgctgaccga
     1561 gaaactgcgc catgcctgcg gagctgccgt cgagtgtgtc atttccgata cctgtatcgc
     1621 gggtatggct gaagccgctg cttgcgaaga aaaattcagc agccagaatg taggcctcac
     1681 cattacggta acgccttgct ggtgctatgg cagtgaaacc atcgacatgg atccaacccg
     1741 cccgaaagcc atttggggct ttaacggcac tgaacgcccc ggcgctgttt acctggcagc
     1801 ggctctggca gctcacagcc agaaaggcat cccggcattc tccatttacg gtcatgacgt
     1861 tcaggatgcc gatgacacat cgattcctgc cgatgttgaa gaaaaactgc tgcgctttgc
     1921 ccgcgccggt ttggccgtcg ccagcatgaa aggtaaaagc tatctgtcgc tgggcggcgt
     1981 ttcgatgggt atcgccggtt ctattgttga tcacaacttc tttgaatcct ggctgggaat
     2041 gaaagtccag gcggtggata tgaccgaact gcgtcgccgt atcgatcaga agatttacga
     2101 cgaagccgaa ctggaaatgg cactggcctg ggctgataaa aacttccgct atggcgaaga
     2161 tgaaaataac aaacagtatc aacgcaatgc cgagcaaagc cgcgcagttc tgcgcgaaag
     2221 tttactgatg gcgatgtgta tccgcgacat gatgcaaggc aacagcaaac tggccgatat
     2281 tggtcgcgtg gaagaatcac ttggctacaa cgccatcgct gcgggcttcc aggggcaacg
     2341 tcactggacc gatcaatatc ccaatggtga caccgccgaa gcgatcctca acagttcatt
     2401 tgactggaat ggcgtgcgcg aaccctttgt cgtggcgacc gaaaacgaca gtcttaacgg
     2461 cgtggcaatg ctaatgggtc accagctcac cggcaccgct caggtatttg ccgatgtgcg
     2521 tacctactgg tcaccagaag caattgagcg tgtaacgggg cataaactgg acggactggc
     2581 agaacacggc atcatccatt tgatcaactc aggttctgct gcgctggacg gttcctgtaa
     2641 acaacgcgac agcgaaggta acccgacgat gaagccgcac tgggaaatct ctcagcaaga
     2701 ggctgacgct tgcctcgccg ctaccgaatg gtgcccggcg atccacgaat acttccgtgg
     2761 cggcggttac tcttcccgct tccttaccga aggcggcgtc ccgttcacca tgactcgtgt
     2821 caacatcatc aaaggcctgg gaccggtact gcaaatcgcg gaaggctgga gcgtggaatt
     2881 gccgaaggat gtgcatgaca tcctcaacaa acgcaccaac tcaacctggc caaccacctg
     2941 gtttgcaccg cgcctcactg gtaaagggcc gtttacggat gtgtattcgg taatggcgaa
     3001 ctggggtgcc aaccacggag ttctgaccat cggccacgtt ggcgcagact ttatcaccct
     3061 tgcctccatg ctgcgtatcc cggtatgtat gcacaacgtt gaagagacca aagtgtatcg
     3121 tccttctgcc tgggctgcgc acggcatgga tattgaaggc caggattacc gcgcttgcca
     3181 gaactacggt ccgttgtaca agcgttaata cattacaccc tgatgtgatg cccggtcgct
     3241 ccggctaccg ggcctgaaca agcaagagtg gttagccgga taaacaatgt tatccggcta
     3301 tattgcagga gcgattatga aacaagaagt tatcctggta ctcgactgtg gcgcgaccaa
     3361 tgtcagggcc atcgcggtta atcggcaggg aaaaattgtt gcccgcgcct caacgcctaa
     3421 tgccagcgat atcgcgatgg aaaacaacac ctggcaccag tggtctttag actgccattt
     3481 tgcaacgctt tgctgattgc tgtcggcaaa tcaatagtga actgactgaa tgccacatcc
     3541 gcggtatcgc cgtcaccacc tttagtgtgg atggcgctct ggtagataag caaggcaatc
     3601 tgctctatcc gatcattagc tggaaatgtc cgcgaacagc agcggttatg gacaatattg
     3661 aacggttaat ctccgcacag cggttgcagg ctatttctgg cgtcggagcc tttagtttca
     3721 atacgttata taagttggtg tggttgaaag aaaatcatcc acaactgctg gaacgcgcgc
     3781 acgcctggct cttcatttcg tcgctgatta accaccgttt aaccagcgaa ttcactactg
     3841 atatcacgat ggccggaacc agccagatgc tggatatcca gcaacgcgat ttcagtccgc
     3901 aaattttaca agccaccggt attccacgcc gactcttccc tcgtctggtg gaagcgggtg
     3961 aacagattgg tacgctacag aacaacgccg cagcaatgct cggcttaccc gttggcatac
     4021 cggtgatttc cgcaggtcac gatacccagt tcgccctttt tggcgctggt gccgaacaaa
     4081 atgaacccgt gctctcctcc ggtacatggg aaattttaat ggttcgcagc gcctaggttg
     4141 atacttcgct gttaagtcag tacgccgtta ccgtaatgcg ctttcactac aagccccgtg
     4201 tcctgacatc atccgcatca atcgttttgc gttttatgaa cgggcgcaaa aagcctttgc
     4261 gatcgttatc acaggcgaac gagcgaagta cgggaatatt cttttaaaaa aaggggtaac
     4321 accgtaatct cataccggta cgcccgcatg acgcgggcgg ttatcgaatg atggggtgaa
     4381 aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc ataccagcct
     4441 gaccacggaa gctctctctg aacagctaaa ggtcagtaaa gaaaccattc gtcgcgatct
     4501 caatgaatta cagacgcagg gtaaaattct gcgcaatcat ggacgcgcta aatatatcca
     4561 ccgtcaaaat caagacagtg gcgatccctt tcacatcagg ctgaaaagcc attatgcgca
     4621 taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg tgatagcctt
     4681 agacgccagt tcaacttgct ggtatctggc acgccagttg cctgacatca acattcaggt
     4741 cttcaccaat agccatccga tttgccatga actcggtaaa cgcgaacgca ttcaactgat
     4801 cagttccggc ggcacacttg agcgcaaata tggctgttac gtcaatccct cgctgatttc
     4861 ccaacttaaa tcgctggaaa tcgatctgtt tattttttct tgtgaaggga tcgatagcag
     4921 cggcgcactg tgggactcca atgcgatcaa cgctgattac aaatcgatgc tattaaaacg
     4981 tgccgcgcaa tcgttgttat tgattgataa aagtaaattt aatcgttcag gggaagcccg
     5041 catcgggcat ctggatgagg taacgcacat tatttctgat gagcgccagg ttgcaacttc
     5101 tttggtaaca gcctgacgga aagggtagca ggccggagac gacgccccgg ccttgcctgt
     5161 tatcgctcgt cgcgacgacc acccaccgca gcccagatgc ggcggacgtg caccgtcact
     5221 tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc atgttcatca
     5281 agctgtgcct gaatatacgc cagattgtgt gatacttctt cgtagcgttt tttcatcggc
     5341 agtttgagat tgaaaatggt ttcacggcac cagccattaa ccagccactg cgccatcaat
     5401 gccgcaactt tagccggttt ttcaaccata tcgcatacca tccaggagat attgctgcgt
     5461 gtcggacgga atttgaaacc gtcttcccgc agccacgtca cctgcccggt atccatcaga
     5521 ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt caccagttgg
     5581 taggtccagc cgccagggca agcgcctaaa tccaccgccc acatcccgtt cgccaggcgt
     5641 tcatcccact catccgcagg aataaacaca tgaaatgcct cttccagttt gagcgtggaa
     5701 cgactcggcg cctctgccgg aaatttcagg cgcggaatgc ccatatagaa cggcgaatta
     5761 ttgttgctgt atgagtaacc ggtatagcag catcctggtg caataaagaa tacatgcaca
     5821 accggacgct tcggcgtttc atagttcgcc agcaccccag catcgcgcaa ggcagcgcgt
     5881 agcggaacag tgaacttacg gcagaatttc agcaactctt tgctttcgtt ggtatcggca
     5941 acttcaacac gcagttcacc gcccttctct actacaccct gtaacatgcc gacaatgggg
     6001 gtaatacgat cttctggcgg caaatgctgc aggagttccc ccaccacaaa ccactggcgg
     6061 gcaaaaatta atgaactgaa cggcagctca cggattaact tatcgccatc atcaggttga
     6121 taacattcat aaatgacata acccgcattc tctttcacgc gggcaaaacc gaaaatttcc
     6181 cgctggccgg ctttatcggt aatttctgcg gcgcactctt tttcaaagcc cggacggcac
     6241 agcaatacaa ccttattcat gacttacgcc cttgcgcttt aaacggatag caccaactaa
     6301 cattaacgcc cagcccgcaa ggaagctcac gccgccaacc ggtgtgacaa acgcccacaa
     6361 acgcagatgg gacagcgcca ggcaataaag gctgccgctg aacagcaccg tgcctaacgc
     6421 gaggaaaacg ctactccagt aaaaccagat actgatgcga cgctgcattg ccaccgccag
     6481 acctaagatc gccagcgtat gaaacgcctg gtattcgaga ccggtttgga tccagcccat
     6541 ctcaacggcc cccatggttt tacttaacac atgcgcacca aaagcgccca gtgccacaaa
     6601 aatgaagccg ctaatagcgg cgaaaatcag cataaaacgg ctggtcatgg tcgtacccta
     6661 cgtaaattat tgttcataac gaaagcggaa tttttcttgt tcagcagcgg ctttcgccag
     6721 gatccattgg cgaaaggcgg ctattttacc cagttctgcc tggctgtcat gacaaaccag
     6781 ataaaaagca tttttactga ccagaacatc attaaacggg caaacaagac gtccggcctc
     6841 gatttcagat tgcgccatca cgttatttgc cagcgccact ccctgcccgt gaatagccgc
     6901 ttgcagcacc atggcgctat gactaaaaat tggcccttgc tgaacgttga tatgattaaa
     6961 ccccaactgt cgggtatatg tctgccagtc acggcgtgac gcatcatgta ataacgtatg
     7021 tttagccaga tcttccgggg tcttcaaggg tttttcgcct gtcagcagta gcggcgaaca
     7081 caccggcaat aaatattcgg cgtacagttt ttccacccgt agccccggcc agttgccccg
     7141 accataaaat atcgccacat caacatcatc cgccagctta tcttcctgac gatcaaccgc
     7201 ctggattcga acgtcaattc ccggataagc tgaattaaag ctggaaagtc gcggaaccaa
     7261 ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac gcccccttgg cgctacgggc
     7321 ctggagttta cgcgtcgctt cggttaattg cgaaaatatc tctttgatat cgaggaaata
     7381 gctttgccct tcctcggtca gcaggagtga acgattacgg cggcggaaca gttttagccc
     7441 caaaaaatcc tcaagagact tgatttgatg acttactgcg gcctgggtca caaaaagctc
     7501 ttctgctgcg cgagtaaaac ttaaatggcg tgctgcggca tcaaaaactc gtaaggcatt
     7561 tagcggtggt aatcgtttag acatggcttt taaactttga tgttaaatga atttaacaat
     7621 tagatcacac tgtgtaacct attagttttt ttaatctgag ccattataaa ttgtccgttg
     7681 agcttctacc agcaaatacc tatagtggcg gcacttcctg agccggaacg aaaagtttta
     7741 tcggaatgcg tattctggtg aacttttggc ttacggttgt gatgttgtgt tgttgtgttt
     7801 gcaattggtc tgcgattcag accatggtag caaagctacc ttttttcact tcctgtacat
     7861 ttaccctgtc tgtccatagt gattaatgta gcaccgccta attgcggtgc ttttttttac
     7921 cttgcgatcg cgaattactg ttccagttcg accatctctt tcacgtcagt acggttgatc
     7981 tgctgtttgt tgccattagc gtctttatac gaaatcatac cgatatcgtt atcagtctgt
     8041 ggtttgccgt cagagacgat ggtacgtccg tcattggtgt gcatcacata gttcgaaccg
     8101 gaacaggcgc tcagggcaaa agtcagcata caggcagaaa taattgcggc agtctttttc
     8161 atcatcttct ccttaaaacg attaataaat cttaatagta ttgattaaca ggctaaaatt
     8221 aacgcctaac actattcagc atatgttact tacgcggctt cgccaggata tccagataat
     8281 tctgatggtt agcactctcc ttgtatcaaa gtgaattttg cgtcacgatc ggtgcatcaa
     8341 gccgaggagt accatgaacg tttttaatcc cgcgcagttt cgcgcccagt ttcccgcact
     8401 acaggatgcg ggcgtctatc tcgacagcgc cgcgaccgcg cttaaacctg aagccgtggt
     8461 tgaagccact cgacagtttt acagcctgag cgccggaaac gtccatcgca gccagtttgc
     8521 cgaagcccaa cgcctgaccg cgcgttatga agctgcacga gagaaagtgg cgcaattact
     8581 gaatgcaccg gatgataaaa ctatcgtctg gacgcgcggc accactgaat ccatcaacat
     8641 ggtggcacaa tgctatgcgc gtccgcgtct gcaaccgggc gatgagatta ttgtcagcgt
     8701 ggcagaacac cacgctaacc tcgtcccctg gctgatggtc gcccaacaaa ctagagccaa
     8761 agtggtgaaa ttgccgctta atgcgcagcg actgccggat gtcgatttgt tgccagagct
     8821 gattactccc cgtagtcgga ttctggcgtt gggtcagatg tcgaacgtga ctggcggttg
     8881 cccggatctg gcgcgagcga ttacctttgc tcattcagcc gggatggtgg tgatggttga
     8941 tggtgctcag ggggcggtgc atttccccgc ggatgttcag caactggata tcgatttcta
     9001 tgctttctct ggtcacaaac tgtatggccc gacgggtatc ggcgtgctgt atggcaaatc
     9061 agaactgctg gaagcgatgt cgccctggct gggtggcggc aaaatgattc acgaagtgag
     9121 ttttgacggt ttcaccactc aatctgcgcc gtggaaactg gaagcaggaa cgccaaatgt
     9181 cgctggcgtc ataggattaa gcgcggcgct ggaatggctg gcagattacg atatcaacca
     9241 ggccgaaagc tggagccgta gcttagcaac gcttgcagaa gaagcgctgg cgaaacgtcc
     9301 aggctttcgt tcattccgct gccaggattc cagcctgctg gcctttgatt ttgctggcgt
     9361 tcatcacagc gatatggtga cgctactggc ggagtacggt attgccttgc gggccgggca
     9421 acattgcgct cagccgctac tggcagaatt aggcgtaacc ggcacactgc gcgcctcatt
     9481 tgcgccatat aatacaaaga gtgatgtgga tgcgctggtg aatgccgttg accgcgcgct
     9541 ggaattattg gtggattaat gacaaacccg caattcgccg gacatccgtt cggcacaacc
     9601 gtaaccgcag aaacgttacg caataccttc gcaccgttgt cgcaatggga agataaatat
     9661 cgccagttga tcatgctggg gaaacagctt ccggctttgc cagacgagtt aaaagcgcag
     9721 gctaaagaga ttgccggatg cgaaaaccgc gtctggctgg gatataccgt ggctgaaaac
     9781 ggcaaaatgc acttctttgg caacagcgaa gggcgcattg tgcgcggcct gttggcggtg
     9841 ttgttgactg ccgttgaggg gaaaaccgcc gccgaattac aggcacagtc gccgctggca
     9901 ttgtttgatg agctgggatt acgtgcgcag cttagcgcct cacgcagcca ggggttaaat
     9961 gcgttaagcg aggcgattat cgctgcgacg aagcaggttt aagcacagaa ccgggtcgga
    10021 taagacgttc gcgtctcatc cgacctgatt gtttaaccct gacgcgccgc tttcgccatc
    10081 atcttcttca gcgcatgaga aaccgcaaca aacccaaagg tggcggtcac catcgttgcc
    10141 gcgccaaatc cagatgcaca atccatcctc ttcggccctt ctgccgtggc tttcatcgca
    10201 cacaccgtac cgtctgactg cgggtacacc agcgcttcag tagaaaacac gcaatccacg
    10261 ccgagcttac ctttactgtt tttcactacg ccaaaatcgc ttttcaggcg ctcgcgcaac
    10321 ttcgccgcca gcggatcctg aatcgttttc gccagatcgg taacctgaat ctgcgtcggg
    10381 tcaatctgcc cacccgcgcc accggtcgtg accagcggga ttttattgcg ccgacaataa
    10441 gcaatcagcg ccgctttggg ccgtacacta tcaatggcat caatcacgta cgaataaccg
    10501 gcactcatat attgcgctac gttatccggc gtcacgaaat catccaccac cgttacacgg
    10561 cactccgggt taatctggcg aatgcgctcc gccatgactt ccgcttttgc cagcccgacg
    10621 ttatcgcgca gagcgtgaat ttgccgattg gtattggtga cgcacacatc atccatatcg
    10681 ataagcgtga ttgcgccaat ccccgtgcgc gccagcgctt ccgccgccca ggaaccgaca
    10741 ccaccgatac caaccacaca aatatgcgcg tcagcaaaca gttgcaacgc tttttcacca
    10801 tacagacgcg ctgtgccacc aaaacgctga cgccatgcat cactaattac cacagacata
    10861 gcacctcaga attaaaaagg gcgaggttat ccctcaccct aaacgcataa atgcctgatg
    10921 cgctacgctt atcaggccta cataactcct gcaatatttt ggatttgcat gatttgtagg
    10981 ccggataagg cgttcacgcc gcatccggta tgaacaacgc gcacttagtc aataatcagg
    11041 atgtgaggtt acccctcacc ctgtcatatc cgtaaaaacg gcatacagaa tatcacatca
    11101 gccgctaaag acgttacctg cgcccggagc cgttttcagc acccaaaccc gtccatagtg
    11161 gttgtaccaa cctgcgcggt gtccggcttc cggcccgatc ccttgataga tatcgaagtg
    11221 ttggcctttg attgcgcccc cgacatccaa cgccaccatc agacgcaatt cgtactgacc
    11281 gttaaatttg ccgttattat ccagcagcgg cacttctgcc agcaaggtag tacctggcgg
    11341 aataattgaa cgatcggagg caacagaagc acggccaacc agcggcaccg cacttgcccc
    11401 tttcaccggt gcaaaagatt gcggtttaaa gaagacgaaa gacgggttct gttccagcag
    11461 ctcgcgaacc tcggcttcac tgtgtgtttc gccccaatga cgaatcgcct gcatcgacat
    11521 atcttctttt ttcacttcgc cacggtcgat cagcacctta ccaatgctgc gataggcatg
    11581 accgtttttc cctgcatagc tgaaaaagtt aagcggactg ccatcaccaa agtcgatata
    11641 cccactaccc tgcacatcca taatgaagtt atccatcaga gagttgctgt aagcgaggat
    11701 gtatttatcg cccaatgccc cggcgtagat ctccgcacga gacggcagac gaccacgttt
    11761 tggcggcata cggtaaatag gatactggaa ctcgccctgg cgggtatggc gcgcctgaat
    11821 taccggcgtg taatagcctg taaactgcac gttaccatag ttgtcggcac cttccatctg
    11881 ccaggcatca atgccgaact ggcgcatatt gcgggtatca ccgcctgcgc gcagccactc
    11941 ttgcaccgcg ttataaacat tactctggtt gccatacaga cgcggtgacg aattacggat
    12001 atggttaatt tgctcggcaa aatcaccggc gttaatcggc gcgccaacgg catctggctg
    12061 gttcaccaga gagaaaggct gggtaaattt cccgtcttta tattgctgtc cgcgatcggt
    12121 tggtttggaa gagcaggcgg caagcattgc cacaaccgtg cccataagaa ggtactttac
    12181 ccaacgtcct ttcattgttc tcttcttcag gttaaaaaat aaggcgcaac gaagataaca
    12241 aaccgccgcg tttaatgaaa tgagcagcct ctccctgacg caaattttgc acaaaaaata
    12301 ggctttagtg gtttgttttt gttcaaaatc atgctaaatc cgtgatcggg gtaaaaaaaa
    12361 ggttgcatga aaacgcgagc ggagtatagt gcgcatccac ggacgcgggg tggagcagcc
    12421 tggtagctcg tcgggctcat aacccgaagg tcgtcggttc aaatccggcc cccgcaacca
    12481 atcaaatttg atgaagtaaa agcagtacgg tgacgcgggg tggagcagcc tggtagctcg
    12541 tcgggctcat aacccgaagg tcgtcggttc aaatccggcc cccgcaacca attattgaac
    12601 accctaacgg gtgttttttt gtttctgatc tcccataaaa aagcgccatt cagcgccttt
    12661 ttatcatccc cttctcgcca gcgttgcccc atcggcaaaa tacgctttaa tccccgcaag
    12721 aatagactcc gcaacttcct gctggaaagt cgccgttttc agtttacgct cttcctcaac
    12781 gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct ttagtacggc
    12841 aaacccggcc tgttcaactt gatttttatg cagcttgttg atttttccga gcttattcag
    12901 taccgcttta ccaaacttaa ggctgtcagc aatagtcagc gactgcacca tatcgaacat
    12961 ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt ccgaggcgtt
    13021 ctgggtttgt gccagatatt ttgccgcagt actggttgcg cctttggttg agagcgcaaa
    13081 caccgaggaa ccgctcggct gacgactggt aaaggcgtcg gcatggatag agacaaacag
    13141 gtcagcacgc tgtttctggg cttttgctac gcgcacttgc aacggaatga agatgtcttc
    13201 attgcgcgtc atgtacacct tcatattgcc ctcttcctcg atcagagagc gcaaacggcg
    13261 agctatttgc aataccacat ctttttcgcg cgttttgtat ttccccaccg caccggagtc
    13321 ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcgcgcc ctgctttacc
    13381 cggttgtgga ccactttgcg ctggcggcac ctgcttttcg aggtcgcctt tgttgtaatc
    13441 ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat agaggtccat
    13501 caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg gttttacgtt
    13561 ttgctttaat tcaaaaacca tgcgtacggt ttgcgggtca aattgcccga cgcgcgccga
    13621 cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta ccgagttcag
    13681 gtttacatct tcgatatcca ccaccacgcg ttcagggtta ctcaacgcga actgcttata
    13741 tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag gccagacgcg
    13801 caccgccacg acctggctaa ccgcagccag gctgacctga cttacgctca atagccacat
    13861 agcgcccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc ctgacatgcc
    13921 tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt gaccggaaac
    13981 tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt ttatattcac
    14041 ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc tgcgaaaaaa
    14101 cagaataaaa atacaataat ttcaaataat catgcaaagg ggtgtgccgt ggtaaaggaa
    14161 cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa tacccaccgg
    14221 ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa tttctccagt
    14281 atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt ggtctatggc
    14341 gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct gtatcacaag
    14401 aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc gggaacattg
    14461 caactggata ttactgctcg cctgtcaatg agtctcaata acacgccgct gcagggcgcg
    14521 catatcaacg tcgttagtgg caattttatt attgcccagc cgctgggcgt cgatgacggc
    14581 gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgct ccatcgtcaa
    14641 ctggaaagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac tggcgagagc
    14701 tttaatctga cctcggaaga gattgccact caactagcca tcaaactgaa agctgaaaag
    14761 atgattggtt tttgctcttc ccagggcgtt actaatgacg acggtgatat tgtctccgaa
    14821 cttttcccta acgaagcgca agcacgggta gaagcccagg aagagaaagg cgattacaac
    14881 tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg cgtgcgccgc
    14941 tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt ctcacgcgac
    15001 ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc aacaatcaac
    15061 gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat tctggtgcgc
    15121 cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca gcgcgataac
    15181 acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg ggaaatggcc
    15241 tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct gctggaacgc
    15301 attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac cacgcgcagt
    15361 attcactggt tccaggaacg tggatttacc ccggtggata ttgatttact gcccgagagc
    15421 aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga tttagggtaa
    15481 gcgatgaaaa tcaccggatg cggcaggcgt aacactcgtg cgtcgcatcc ggcaattacg
    15541 tttattcccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta cgagtggcga
    15601 ttgccgcact taatatacgc tcatcggcat acagcgacag acgacgacgc gcgcgggtca
    15661 ccgcggtgta taccagctct cgcgttacta ccggcgtgcg ttggctcggt aaaatcaacg
    15721 ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac gtagtttcgt
    15781 gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc gcaaaccaga
    15841 cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac aacccaagcg
    15901 cgctgtcatt acgggcaatc atcaccggtc ggccttcgta ccaacgagag tgcggatgac
    15961 gatgaatttt gcgcttctgt tgcataaact gttcaattcg ctcattcagt ccagccacgc
    16021 caaacggccc ttcccgcagg gcgcacaaaa gctggtactc attgaacgcc tgaatgatta
    16081 aatccggctc ggcacgcgct tgcagcagat ccagataacg tccgtaaccc gcaagagctt
    16141 cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag tcgtttttcg atatcagtaa
    16201 aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc gccgcagcta
    16261 actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg cagagactgt
    16321 cgcgcaaaga tgccgcttct gtgccggttc ctgccggaac gtgagtcccc gtcaggcggc
    16381 ttagctgcct ggcacgctcg gcggtaaagc ccgcgttggc ataagcgcag atatcgccca
    16441 gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag atcactcgcg
    16501 catgatctgg caaggcgtcg atcagtctcg acatcatagg cagatcgatc attgacgctt
    16561 catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt aaacgctggc
    16621 tacccggctg cgcgcccagc aatcggtgca aagtgctggc atcttccgga atgcgtttct
    16681 tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg gttaagcgcg
    16741 cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg tcggccattt
    16801 gaattaacgc tgccagcaac ttcgccacgg tggtcgtttt accggtgcct gggccgccgg
    16861 aaatcaccga gatccgccgc gtcagcgcca ctgccgctgc aactttttgc cagttaattt
    16921 catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca tcaacctcaa
    16981 tggtatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac cacatgcgat
    17041 tcaaataaag acgatcgcca cagaggatca tcggcgtggg ttcatctccc ctgctgaccg
    17101 cttgagaagc cagcaagcat tcttcccaat tttgtagctc accgatttca ctgacacagg
    17161 tcgccaacag cggattcgac gcctcgttat tttccagtcg tgaaagcggc aaacagacgt
    17221 gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca ggatgttcat
    17281 ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg tgctccacag
    17341 cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac cggcaaacat
    17401 ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc cttgttgcgg
    17461 atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt gaagatcata
    17521 gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat aaagctgata
    17581 ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg tgtaagccga
    17641 actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc cttcgtggcg
    17701 gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact ccagcggcgg
    17761 gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc tggcgatgag
    17821 cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt cggaaagctg
    17881 actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga tccactcggt
    17941 caacaccggt tcccactgcg attcaaagcc gccgagttcc agtttttcct gtacccagtt
    18001 cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga atgtccccgg
    18061 tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga cgctgaccac
    18121 gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt gaccacgctg
    18181 ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt gtagtgtcct
    18241 cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctggttat caccagtttg
    18301 tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc gaagccctgc
    18361 cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt ggacgtcggt
    18421 gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac tgcaatgcca
    18481 aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac gttcgacctc
    18541 cgcgaggtcg acgctttctg gcgcagcatt aagatccaaa actgcctcaa acgagtggcg
    18601 atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca accagaccag
    18661 cggatattcc agccctttcg atttatggat cgtgacaatc tgcaccagat gtttatcact
    18721 ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat gttgcgataa
    18781 ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgaa gcagttcgct
    18841 gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca agttttctgc
    18901 aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt tgcgccagat
    18961 ttgccgataa ccatcgaact cttcgactac cgtatcccac gcatgttcgt cattattcag
    19021 cgtttcaata tccagcgcat tcagccccat cattgacgtt gccagcgcac tgcgcagggt
    19081 gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct gcgcttccag
    19141 agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca gcaacgttaa
    19201 ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacgctga tgtccgaagc
    19261 acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct gtccggcttg
    19321 tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat agtcgccaac
    19381 gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt cacctttaaa
    19441 tacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg gtatttcgcg
    19501 aaacatgaac gtgtcatcag tctggctgaa aagcttgttc acactgttca ccattccagg
    19561 tgcggagcgc cagttggtgt ctaaagtgta gtgggcgtga acttcactac gcgccttcat
    19621 ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt cgccaatcag
    19681 caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt actgctgggg
    19741 gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga tcgccgctgc
    19801 caacacctcg ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca tgtcatcaaa
    19861 acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct cagccaatgc
    19921 gcgggtgatc aacagatcgc ggatcgacaa tggttctgca agcagttgat cgatcgcctc
    19981 aaacagtgga tgtcgcgggg tttccccccc ggccttcgtg cgatcttcta agaaacgctg
    20041 ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt ctgcccaggc
    20101 gctgatcttt tcgatccatt tagcctgatt gctacggtta aacttgcgtc gatcaatacc
    20161 agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct gttttaccgt
    20221 atcaatacgc gccacaattt gcgcgtggcg ggaagccagc gtttcatcat cgggcggcgg
    20281 tgctttgata accggcggtt cgccttgcag ataacgatta atatcgcgca gcaacgcctg
    20341 cggccctttc caggtttcaa agacgacctg ggctatttca cgcggcagcg ggtagcagtg
    20401 gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt caatcagctg
    20461 ctgctcaaac agcatgccgg attcaaaggc attcaggttg agcatgcgct ggcaaaagcc
    20521 gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca accactgcgc
    20581 ggcttgcgct ttatcgtcga tctcttccag caggcgttcg tacagtggat tgtcggtggt
    20641 ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac cgcgcaattc
    20701 tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacgatca gcgggcgggg
    20761 aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg caatcgtaaa
    20821 ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg gcaagcgcaa
    20881 aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc attgtttgct
    20941 gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat tcggctttcc
    21001 cgttctggct gccggaaatc tgcgacagaa tagccatgcc ttgcggctcg accaccgcct
    21061 gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc acgattttat
    21121 cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc gatgcttctt
    21181 cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg atttgcgcaa
    21241 actcatctgg cttcatcgct cgcaattttg cctctgcggt tgggaaaaac gccttgtaac
    21301 gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc atgccccact
    21361 gacgccccac gctcattgga aacgcaaaca cggcatagcc caattgttct tcggtacgca
    21421 actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag gctgagctgg
    21481 tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg gtgctgttac
    21541 cggctttttc aaagatgacg gattgttttt tatcgaccac tacatctttg tttcgacacc
    21601 actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt gttgcctggg
    21661 cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt aaggcgtcgc
    21721 gataggccag cacctctttc aacgtaatgg agggcaaaat tttacgccgt tcatctcgcg
    21781 agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca aacgctttac
    21841 ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca agctgctctt
    21901 ccgtagcggt atagctaaag tacccctcga gcaatgcctg gaacagctgc ggcagacgct
    21961 gggtgtaacc attagcatta atcataaggc cgttgttagc gttggtggaa aaacttatgc
    22021 caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga taatcattga
    22081 gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc aaaatcaggc
    22141 tgacatcagc tttgggctcg ctggcaaaat aacggcttgg cgcatacacc acgcgcagat
    22201 tcgactcatc aacaatcagc tctggatggt cgtatttctt ctctgactta atcagcgaga
    22261 aatcatcagg aatataaggg ttaagctctg gcaaagagag cgcaatgtcg gcggcttttt
    22321 tctgccagtc ggcgaaagtt tgtgcgctga ttttatcgac ctgatacggc gcatcgacaa
    22381 agtaagccgt tttgttgtgc ggctctttcg ggctgatata ccagatacgc gcattctgcg
    22441 gcatcatcat cgccagacgt tcctttactg ctttagcatc gtaccgatcg gcaatattga
    22501 ctgcatccag cgtatgctca acaggaacgc gaatcatggt atctgccagc cattcgacgt
    22561 aatccatatc acgggtgatc gacggataac ggaagtcgat atccagcaca ttcgccagtt
    22621 catcgaagta ttgtttatca atgccttttt cacgtaacag attgagatag ctaaaaattg
    22681 ccgccacaac ctgatcgcga ttagccaggc ctttatcagt taaagacgca gagatcgcta
    22741 atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc tcaactaatc
    22801 cctgcttttg cagccagtca gaaagtgtac ctgggctgcg attgccaatc agataggtaa
    22861 tcaattcatc ggttttacta cggaacttcg ctgagttgtt atcgatgcga aactcaacgc
    22921 gcaacacttt acgcggcagc gcagggacgt aatgaatgat aatgcccttt tgcgcgtcgg
    22981 tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg cgaccaaagg
    23041 tgtccgccgc catttttgcc aactccggca gcggtttatt actgtaaata accgccttca
    23101 tcaaattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc accggattac
    23161 caggtttgtc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg gggtgtgccg
    23221 ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta cgcgccatgg
    23281 ttaattcagc gttcactgca ttacgctcac gttcggcgta tttcttgtcg agcaagggtt
    23341 ctgcaatagc atcggccagg cggtctaccg caccgggcaa ggcgtcgttc tcaacttcca
    23401 gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg tgcattttga
    23461 gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga ctcatatgtt
    23521 caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca acgggcacta
    23581 ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc accataccgt
    23641 tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg atggtttcct
    23701 gaatcggctg ccatcccgtt tctgcctgac ttaatggtgc ccagagggca acaaacaaca
    23761 ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa ctttattttc
    23821 tggacagatt caacaggtta acgcaaatca tctgccagaa tttaatcttg tgctgcacga
    23881 gtcagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa agtaagcgga
    23941 tagattgcgc aatttttata caacactcat gactgattaa agcgaaacag cggtaacagg
    24001 aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg ccgccagagc
    24061 cgttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc gtaagcctga
    24121 aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc atcgttttgc
    24181 gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag caatggcgcg
    24241 gacattcctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc ctgttcggct
    24301 gcaagcggcg gaaaacgcca ctcaccgtct ttgcgtagaa aaaggcgact ttcgccatta
    24361 ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg cgccacactt
    24421 aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa ccagccagtt
    24481 atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg gcgacaggca
    24541 atgactctgt cggcaagctg ctgcatctcc tggcactgtg tttcccagaa aatttcacca
    24601 aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg ttcggcatca
    24661 tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct aagtccttcc
    24721 agaataaatg gctcggtgtc tgggatttcg ctgtcttcag tatggaagtt cacctgcaaa
    24781 cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt ttccagcggc
    24841 acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc tttaccagcc
    24901 tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg ctggtagttt
    24961 tgcggatcaa acggcatccg ggtatggagg caagtaagat gcgcttttac ccttgcctcg
    25021 ctttcatcac agttgagcgc ttcatcgccc ggtagataat gactttgccc gatgtagtcg
    25081 atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat ggaacgcccg
    25141 atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa caggtagcgg
    25201 tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag gtcaaagccc
    25261 aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca aaccactttg
    25321 aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc gagaaaacgc
    25381 tggctgatac gttcttgatc cagacgctgt gccagttcat cacgcaatag tgacagcggc
    25441 accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca ttgttgttcg
    25501 atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt gagcatatcg
    25561 cgacaaaccg gcaaccactc ttctagcgga cgctcctgtg ccagcccgcg acgccagatg
    25621 tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa gccgctcgat
    25681 tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc gtagcccagc
    25741 aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag ttccagctcg
    25801 cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca ctggcgtaaa
    25861 taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac atccagcagc
    25921 gccagcacat cctctgacac aaagcgactg tcaggcagtg ataacaggct gataaacgct
    25981 tccagtaccg gatgtgactg ccgcgcacga cggtcggaaa tggcgtaagg caggtaacga
    26041 tccgcaggtg cgctaccaaa cactgcctga ataaacggac tgtagctgtc gatatcagcc
    26101 accatcacga tgatgtcgcg cggagtaagt gtcggggctt cctccagcat cgccagcaag
    26161 cgatcgtgta aaacttcaac ttcacgctgc gggctatggc aaacgtggaa tgtgatactg
    26221 ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc gatgttcaca
    26281 cccgcaacgg cgcggttttc cagttccaga atgtcagact gaatgttatg cagcaggtta
    26341 tctggcgtca catcgacaaa agcgtccagc tcctggctgc tctccaggtc agaaaggaga
    26401 taaatgtagt cgcgcccaag cttcccccat gaggccagca gcgagttgcc gacatcctgt
    26461 tcaccatcgc tgttaaagag ctgtccggca ttttcgctat cgcgaaatag cggcaattcg
    26521 cgatcttcaa aactgtgtcg acgctgacga gtcagcagtt tcgccagata agcaggatct
    26581 ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat ttcaatatgt
    26641 ttacccagcg cctggagcgc ctggagataa acaggcggta acgcggaaat accgcatata
    26701 aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt ttcgataaag
    26761 cgctgataga gattcgcgcg gtgccagcgc ggttgtccga gttcatgggt atattccacc
    26821 agcgccttcc acaacggggc ttgccaggcc tgtgcttctc ccagcccttc aaccaaatgt
    26881 cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc aaacaggtcc
    26941 gccgcttttg aggaaagctg gaacagtttt cgcttgtcgc tatcgtcagt cagataatgc
    27001 cgcaacaggg taaagtcttc gcgctccagc aattgcggca gcagagtcat cagtttccag
    27061 ctcatgctct gtttgttaaa ggcgctctct ttggggattt ccggtaacac ccggacgaac
    27121 atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat accaaacttt
    27181 tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag aatcatctct
    27241 ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa cgcttccagc
    27301 acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta ctgacgattc
    27361 gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag cgtaacgctg
    27421 atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt ggcaggtggc
    27481 gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg gtactggttt
    27541 ctgctggcga gactgttcat tgatgttcgc tggatacccg ataacgcagt gacaatcatc
    27601 accatcagca ccatcagcac catcgcaacc attacctccg gcaggctaaa gccttgttga
    27661 ttcttcaggg aagctgacat aatgccccct ctttcagcgg acaaaaatcg ctccagccgc
    27721 gtggcgaaaa aacaattttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt
    27781 cataaccggc aatcaataag gcttcattat ctgccagtaa acgcaaacaa acctgggcat
    27841 cggttccagc gtactgcgag cactgaactg ctggctgcgt ctgccaggag tgcatttttc
    27901 cccacgccag cgccgactga acgatggcct ggcggcgcaa tgactgactt tccatgctca
    27961 cgcgagaggc aaaactgcga tcctgctgac tcattccttg caatagcaag ctacccaaaa
    28021 ccagcagcat caggaccaga gccagtgacg aaacaccttt ttcgcggttc accggttgaa
    28081 tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg
    28141 catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt
    28201 gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt
    28261 cgcaccgcgt agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcggactc
    28321 ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca
    28381 gtcaccctgt ccgacaattt ccagcccttc gccggtacag ctgccatgac aataacccgc
    28441 cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagtgccag
    28501 cttacgagtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccag
    28561 caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc
    28621 ttttacaggc atccttctgt ttctccttgc tgataaagcc ggagtcttcc ccacggcgaa
    28681 accaccagcc accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta
    28741 ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag
    28801 cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc
    28861 acaaggcacc ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccag
    28921 ttggcatctt cacgtaaata gagcaaatag tcccgcgcct agctggcggt ttgccaaagc
    28981 cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa
    29041 atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca
    29101 gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg
    29161 gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa
    29221 cggagggtaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag
    29281 atagccaccg gcgctttaac gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg
    29341 aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc
    29401 ggttcgcggc ttaattgcag atgagtttga tccatatggt tgctgtacag gtgcgtatcg
    29461 ccaccggtcc agacaaaatc acccacttcc agatcgcact gctgcgccat catatgcacc
    29521 agtaacgcgt agctggcaat gttgaacggc aggccgagga agacgtcaca ggagcgctga
    29581 taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaatgc atggcacggt
    29641 gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa
    29701 tccgggtcgt ttttcagctg gttcagtacc gtagtaatct ggtcaatatg acgaccatct
    29761 ggcgttggcc aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc attttcatcg
    29821 gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtatcgccc
    29881 tgcaggaacc acagcagttc gtggatgatg gaacgcaggt ggcaacgttt ggttgtcacc
    29941 agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg
    30001 gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa
    30061 tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc
    30121 atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac
    30181 tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga
    30241 aacgcgccgt agccaatcag gaacaagcct gagacagctc ccattgggcg cggtttacga
    30301 atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagttc gtaaagctgt
    30361 gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt
    30421 tgcagcagca aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc
    30481 gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga
    30541 ccaaacggaa tgagtggtgc gataaaatca gagacctgga agaaggaacg tttagtacgg
    30601 cgggcgaaga taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg
    30661 ccgtcccaga cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac
    30721 agaacataac caatacgtcc cccgaggaag acgccgagga agcccgcata gagtaagttt
    30781 tcaacttcat ttttggtcca gccactgccc ggacgattcg cccgacgtgt tgccagccac
    30841 attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct
    30901 attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac
    30961 cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc
    31021 aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt
    31081 aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga
    31141 cgctccataa aggctgcaac ctgatgacga acttcggtcg ccagttgcgc ttccagacta
    31201 cgctgcgcaa gattttctgc ttcggcaaaa tcaatgcgcc gcagcaggta ttttacccgc
    31261 gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg
    31321 cacatgggat cgcccgccat ttcaccgcac aaacgaagat cgataccatg tatttccgct
    31381 tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc gtaaatgttc
    31441 gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca
    31501 acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact
    31561 tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca
    31621 cgtccggcac gttcaatcag gcggcgtgct tcgtcaactt catcgaggct ggtgaccatc
    31681 ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg
    31741 atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga
    31801 ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc
    31861 aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct
    31921 tcttccgacg ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg
    31981 ccatcaatac agctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg
    32041 acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga
    32101 cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc
    32161 ggatcgacca gcaattcacc gcgatagcca tcaacgatca gcgtcctacg atgcagcacc
    32221 gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca
    32281 gcatgggagt tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc
    32341 tcagcaagag ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg
    32401 ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa
    32461 tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt
    32521 tcaatgaccg tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat
    32581 tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt
    32641 tctttttgtg ctccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct
    32701 tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac
    32761 acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt
    32821 gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacagggc agtcaactgc
    32881 gactgagaaa gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt
    32941 tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt
    33001 tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat
    33061 ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc
    33121 aggccgacga tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgt ttttttcagc
    33181 ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag
    33241 acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg
    33301 cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg
    33361 acttaacctc ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc
    33421 agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat
    33481 gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt
    33541 gtactgctgg tttgcatatt gatttctgca tcgccgctta ccagctgcaa gagaaaccat
    33601 ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat
    33661 ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct
    33721 acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg
    33781 ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg
    33841 cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta
    33901 cctcaaaata aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca
    33961 ctctctgcag ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt
    34021 ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg
    34081 aagaactacg gaattactac gggaaaaccc gaagcattct gaataagccc gatatagaaa
    34141 ttttaaaata ttcattatta gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta
    34201 attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta
    34261 tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc
    34321 gcacgtcaag gctgtaaatg aaaacagtag tggaggtttt tcacagttat cccagctttc
    34381 tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacctatt
    34441 gcagtgttgc gcaactcgat taccggcagc ctaaaaagca atataaatca gagaattgaa
    34501 caacacatgt ggaaaaagtt acactgcgaa tattcggcac ataattactg tttgtttttt
    34561 aaccaaggta tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag
    34621 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
    34681 ttgccgggct ggttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac
    34741 tggagatctg gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gctgctctgg
    34801 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
    34861 tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg ggaaaccagc catgtgcgcc
    34921 acaagctcaa acgcgtactg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc
    34981 agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga agaggaagac cgacagctac
    35041 gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag cggatcaccg
    35101 ctcgtcacgg ggaatattta cagataagac cgaaagcagc gaatgcgaaa gcgcttaccg
    35161 aagccattgg tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga
    35221 agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac
    35281 ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt
    35341 atttgcatgg ataaccgatc ctaacgcttg gcttgcgctc ggtacgctga cgctgctgga
    35401 gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa agcttcccac
    35461 agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc
    35521 gctgctggca tcaatctctt gggttacgcg cctgacaaat ccgcttttta caatattcag
    35581 tcaggaaatt tctgcccgtg atttgattct gcttctgggt ggcttgttcc ttatctggaa
    35641 agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt
    35701 ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct tcagcctcga
    35761 ctcggtgatt accgctgtgg gtctgtcaga tcacctgttt attatgatgg cagccgtggt
    35821 gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca
    35881 tccttcggta aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct
    35941 ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat
    36001 tgcggttgaa agcctcaact tgattcgcaa caaaaagaat ccgctctgat actccgtacg
    36061 ctctcctgcg ggagggcgta ctcactcctt ttaccctcac agtttaagat tttcctgctt
    36121 tcaagatata taacgtcggt ttataaacag actattatca taggtaggcc aaatgaggat
    36181 aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc
    36241 gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga
    36301 aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg
    36361 atgcaaatta accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca
    36421 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
    36481 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
    36541 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
    36601 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
    36661 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
    36721 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
    36781 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
    36841 tccgcgaagc gctgcatgac agcctcaagc gcctacagac tgattacctc gatctttatc
    36901 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
    36961 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
    37021 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
    37081 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
    37141 tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac
    37201 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
    37261 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
    37321 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggcctt gatcctgctc
    37381 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
    37441 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
    37501 tattagctga aattgaagcg gtacatcagg tttatactta tcctgcacca taaagattaa
    37561 cgccggttaa aaccggcgtt aaatattaat gacggcgctg ccagatccac agcgccgtta
    37621 ttgccagcgc aaatagcgca ccgaagccaa tgccaatggg cacgaccggg atgcctacca
    37681 ttaccgccag cgagtaaatg cccagcatca acaacatggc gctgttttcg ccaaggtttt
    37741 gtactgcaat cgcattcccc gccccgacgc tttttttacc ccgctcctgt agcaacgcat
    37801 tgagcggaac gacaaaaaag ccccccagca cgccaatcag catcaacaag gcataggctg
    37861 gcagcagctc gtgttgcagg gagaaaatcg gtaccaccac gccaatcaaa atcccggctg
    37921 gcatacagcg tgacacggtt tccagcgtaa ccagcttcgc tgccgcacct gcgccaacca
    37981 cgataccaat cgctaccatc gcgttgagat aggtcggcgt agcgttatcg gtaatgccca
    38041 gcgccaccgg tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata
    38101 aactggtgcc caccagcgaa aaacgcgttt caccattgca ccatagcgag gtgcaggcat
    38161 tcaggaaact gcgggtcatg ttgatgagat tccaggactg ccccggacgc gccgccagtt
    38221 tgggaatgta gatattggca acgaccgcac caccgtaggc cagtgcgcat gcggccagag
    38281 cgaccaggac atgccagtca gccagcacac caccggctac ggaaccgagt aaaatcgccg
    38341 ctatggcaga agcttccatt aaaccgttag cttttactaa cttactaccc gtggttaatt
    38401 cgccgagaat accgtatttc gccggtgaat aggctgcagc accaacaccc accagcgtat
    38461 agccgagaaa cggattgata ccaaagcaga tactggctgc gcccagcagc ttcaggccgt
    38521 tggcaaacat catcacccgg cctttggcga agctatccgc cacctgcccg acaaacggcg
    38581 ccaaaagaat gtaagcacct acaaacacca tttgcaggat gggctggctc cactccggat
    38641 agaactgcgc tttcagtaac gccagagtgg caaacagtag cgcgttatcg ccaaacgcag
    38701 agagaaactg cgccacgata accgctttca tccccttcga ccacaacgaa gtgttagtgt
    38761 gcactgactc actcatcgtg ttgttccgct tcgtctaccc agcttttcaa cgtgacaaag
    38821 tcaggtttgc cgctgccaag taatggcatc tgtttcagat agcgaatatc gcgcggtaca
    38881 gcaagctccg gcacgccgtg ctcgcgggca tactgttgca acttatcgcg cgtcagttcg
    38941 ttatctgtgg tgaaaagcac cagtgcctcg cctttgctgg catcgctctt aatcgcagtg
    39001 gcatggactt tatctggcga aacaccaagt gccagttgtt ccaccatttc cagcgacacc
    39061 atttcgcctg caattttggc aaagcgtttt gcgcggccct gaatctgcac aaagccctgc
    39121 tcgtcaaaac gcacaatatc gccagtgtca taccagccgc gctccatttc gccgcgaaca
    39181 ttctcggcgg tgggcacttc cagtacacct ggcttctcca cccgcagata gccgttcatt
    39241 atgttcggcc ctttcagttg caggcgtccg ccctcttcga taccagggac cgacaacagg
    39301 cgtgcatcca ttcctggtag aatacgccct accgtaccgg gtttcgccgc catcggtacg
    39361 ttgatagaaa cgacaggcgc gcattcggtc acaccgtagc cttcaaggat gcgcaggcca
    39421 aatttatcct gccaaagctg tttggtactt tcttgtagtt tttctgcgcc tgccaccaca
    39481 tagcgtagac gatagaagtc atacgggttg gcgaaacgcg cgtagtgacc gaggaaagtc
    39541 gaggtaccga acaacacggt gcaactgcgg tcatacacca agtccggcac aatgcggtaa
    39601 tgcagcgggc ttggataaag gaacacttct gcacctgtaa gcagtggcgt aaacaggcct
    39661 accgtcagcc caaaggagtg aaacagcggt aatgccgaca taaagcgatc gttggtggtg
    39721 aagtcggcaa tcgttttaat ctgctcgacg ttcgccagaa tgcttttatg gctatggacg
    39781 acgccttttg gatggccttc agaaccggag gtaaagagga tcagcgcctc ttcttccggc
    39841 tgctgtttta cctgcgccag gcgcggcatc agcaaatgag cgaagatcca tactttgtcg
    39901 gcagtggtga catctgcttt taaatcttcc agatagaccc agcgcacctg agtaagttgc
    39961 tccggcagat gccagagttt gcctttatcg agaaactggc gggaagtgaa gatggttttg
    40021 atttcagccg ccgtaatagc actggtcagc ccttttaccc cggcagtgta gttcatcatt
    40081 gcgggaatgc ggcgacgcgc gatggcccca aaaatcactg ccgcactgat gcctgcattg
    40141 ggcagcatta agccgatgcg ttcgccttca acactgtatt tttcaaggat gcgtccaaca
    40201 aacagcgttt tcgtaagcaa tttgcgatag gagtctgggg taaagttgac gtcttcgaca
    40261 catttcttcc cggctccgaa gcggtacatt gcactcagta aagattcgta cagagtttca
    40321 cgcgggcgca ccgccatacg cgcttccatc attatttgat gcagcatttc gccagcgatt
    40381 ttgcgacggt cacgggcacg cggcgcatcc ggcatcgcca cctgcgttgg aggcaaaata
    40441 tgcagagtaa tttgcgggaa caagcgacgt ttaaccagac ctttcaggcg gctgaagtgc
    40501 gtaagttccg ccccttcaat acgcacagga ataaccgttg caccagactt cgccgcgaca
    40561 aaacccgcgc catcgtagat tttcatcagc gagcctgtag tggtgatgcg tccttcaggg
    40621 aaaatcacca ctggtcggcc ctgttccacc agacgtacca gatgtttaat agccataggt
    40681 tgcgtcgggt cgagaggaac aaagtcgata aatgatttca gccaacgcat ataccactgt
    40741 tggcttattg aggtgtaaac ggcaaacact ggacgcacag gtaaaaacag tccaagcaaa
    40801 atgccatcaa taaaagagac gtgattaggc gtaattagta cgcgctcgcc cttcagtgcc
    40861 tgggtgtcac ccgtaacgcg aacgcgatac aaaacacggc acaaatttcg aaaaaagcta
    40921 aaaagcattc caactccctt tgctctgatt cagtaaaagc gaatggaggg agattacacg
    40981 agataaagaa cgcgagcgac agtaaattag gtgcgaaaaa aaacctgcgc atccgcgcag
    41041 gttggtgcaa gagacagggt acgaagagcg taccgaataa tctcaccaat caatacctct
    41101 gggatcttga ttgtggtctg cacgacgact cttcgccagc gagaaaacgc aaaggaatga
    41161 agggaaatgc aacgaggtgt gtaaattgtc ggttactgtt acagattgat gaccggcaaa
    41221 aaaaaacctg cgcatctgcg caggctggtg taattcatgt gctcaacacg aagttgactt
    41281 cacctatcaa tacctctggg atcaccactt tagcaacctg aagccaaacg ccaccagcgg
    41341 tcaatcgcaa cagcgtttcg caaagtgtaa ccaaaggttt gcaatctctt tttttgtctt
    41401 acttatttgc gtttggctca cattcccacg atgaaaacac gccacccctt gaaccaacgg
    41461 gcgttttccg taacactgaa acaatgtaag cgtttaccca ctaaggtatt ttcatggcga
    41521 ccataaagga tgtagcccga ctggcaggcg tttcagtcgc caccgtttcc cgcgtcatta
    41581 ataattcacc caaagccagc gaagcttccc ggctggctgt gcatagtgca atggagtctc
    41641 ttagctatca cccgaacgcc aacgcccgtg cgctggcgca gcagaccact gaaacggtcg
    41701 gtctgatcgt tggtgatgtt tccgatccgt ttttcggtgc aatggtgaaa gcggtcgaac
    41761 aggtggctta tcacaccggt aatttcttat tgattggcaa cggttaccac aacgaacaaa
    41821 aagagcgtca ggctattgag caactgatcc gccatcgctg tgctgcgttg gtcgtccatg
    41881 ccaaaatgat cccggatgcc gatttagcct cattaatgaa acaaatgccc ggtatggtgc
    41941 tgatcaaccg tatcctgcct ggctttgaaa accgttgtat tgctcttgac gatcgttacg
    42001 gtgcctggct ggcaacgcgt catttaattc agcaaggtca tacccgcatt ggttatctgt
    42061 gctctaacca ctctatttct gacgccgaag atcgtctgca agggtattac gatgcccttg
    42121 ctgaaagtgg tattccggcc aatgatcggc tggtgacatt tggcgaacca gacgaaagcg
    42181 gcggcgaaca ggcaatgacc gagcttttgg gacgaggaag aaatttcact gcggtagcct
    42241 gttataacga ttcaatggcg gcgggcgcga tgggcgttct caatgataat ggtattgatg
    42301 taccgggtga gatttcgtta attggctttg atgatgtgct ggtgtcacgc tatgtgcgtc
    42361 cgcgcctgac caccgtgcgt tacccaatcg taacgatggc gacccaggct gccgaactgg
    42421 ctttggcact ggcggataat cgccctctcc cggaaattac taatgtattt agtccgacgc
    42481 tggtacgtcg tcattcagtg tcaactccgt cgctggaggc aagtcatcat gcaaccagcg
    42541 actaaccgca gttaaagcaa ttccagcgcc agtaattctt cgatggtctg gcgacggcga
    42601 atcaaccgcg cctgaccatt atcaaacaga acttctggta acagcggacg gctattgtag
    42661 ttggatgaca ttgatgcgcc atatgcccct gtatcatgca gtaccagata atcacctgcc
    42721 ttcacttccg gcaaggcgcg ggtttcaaca tttccccctt cctgctgggt aaagacatcg
    42781 cccgattcac ataacggccc ggcgacgacg gtttccaccg ttggcgcgtg ttccagagaa
    42841 cgaccatcag ctgccagggc actgatatgg tggtaactac cgtacattgc cgggcgcatc
    42901 agatcgttga acccggcatc aaccagcaca aagtggcggc tacccatttg tttgacgctc
    42961 cgcacctgag taattaatac gccagactgc gctaccagga agcgacccgg ttcaatttcc
    43021 agtttcacag ggtggcccaa atggcgagcg atttgctcac gcgcggcatt ccacagacca
    43081 taataatgtt cggtatcaac cgcctcttca ccctgttgat aaggaataga aagcccaccg
    43141 cccgcagaaa tagcctgtaa atcctgaccg aattcgataa cctgacgcac catagcacca
    43201 cacacctgct ccagatgggc ataatcaacg ccagaaccaa tgtgcatgtg aatgccgact
    43261 agctgcagat gatgacgttg tatcacgtcc agtgcggcgg gcagatcggt gtaccagata
    43321 ccgtgcttgc tgttttcgcc accggtattg gttttttggc tatgtccgtg accaaacccc
    43381 ggattaacgc gcagccatac ccgatgccct ggcgaaacct ggcccagttg gtcgagcgta
    43441 tcaacagaac ccgcattcac cggaatttgc aattcactga cgcgttcaag cgtcgcctga
    43501 tcgataacat ctgccgtaaa aacaatatca tcggggtgcg tttgcggatt gtaacccgcc
    43561 gccaacgcac gctctatttc gcctaacgag acggaatcca ctttcacgcc ctgctcacgc
    43621 attaagcgca aaatatgaat attggaacag gctttctgtg caaagcgcac cacttcaaac
    43681 tgtttcagcg ctgcaatctg ccgacgaata atttgcgcat cgtagaccca caccgggcag
    43741 ccaaattcag cgggcaaacg cagcagattt tcggcggtga gatcggtatc ggtgctgaac
    43801 agtgaatgtg gcataacaaa ctccagatga gtgctttttt atgattacgc cacatcataa
    43861 aaagaataaa aaatatcgat ttatgtcgag tctatgcaaa aatgatatgg attaccggat
    43921 tgcgagagag cgctaatggc cgccgttaac ttacgtcata ttgaaatttt tcatgcggta
    43981 atgaccgccg gaagcctgac tgaggcagca cacctgctac acacctcaca gccaaccgtc
    44041 agccgcgaac tggcgcgctt tgagaaggtg atcgggctga aattgtttga gcgcgtacgt
    44101 ggacgattac atcctaccgt gcaaggactg cgtctgtttg aagaagtgca acgatcctgg
    44161 tacggactgg atcgcattgt cagcgccgca gaaagtctgc gcgagtttcg ccagggagaa
    44221 ctgtctattg cctgcctgcc ggtcttttcg caatcttttt taccgcagct cctgcaaccc
    44281 tttctggcac gttatcccga tgtcagctta aatatcgtgc cccaggaatc accgctactt
    44341 gaagagtggc tctcggccca gcgtcatgat ttaggactca ctgagacact ccatacgcct
    44401 acgggaacag aacgtaccga attactctct ttagatgaag tgtgtgtgtt acctccgggt
    44461 catccgctgg cggtaaaaaa ggtattaacg ccggatgatt ttcagggtga gaattacatc
    44521 agcctttccc gtactgacag ctatcgccag ttgctggatc agctatttac tgaacatcag
    44581 gttaaacgac gcatgatcgt agaaacccac agcgccgcgt cagtctgcgc aatggtacgg
    44641 gcgggggtag gtatttcggt ggttaacccg ctcaccgcac tagattatgc ggcaagcggt
    44701 ttagtggtgc ggcggttcag tattgcggtt ccgttcaccg tcagcctgat ccgccccctg
    44761 caccgcccgt catcagcgct ggttcaggcg tttagtgggc atttacaagc ggggttaccg
    44821 aaactggtca cttctcttga cgctattttg tcgtcagcta cgacagcata aaagcgacag
    44881 catcctcggc atggatcgcc gcggtatcaa acacaggcaa aacactgcgc tcttctggca
    44941 ccagtaaacc aatttctgtg cagccaaaaa tgacgccctg tgcgccctgt tctgcaaggc
    45001 gagcaatcac ttgcgcataa taagcgcgtg acgcttcggt aaattgcccc agacacagtt
    45061 cttcaaaaat aatctgatta attttcgccc gttcatccgc ttcaggaata agacaattga
    45121 tggaaaattg ttccgtcagc cgcccgcgat aaaaatcctg ttccatggtg taacgcgtac
    45181 ccagcagcgc cacacgagtc attccggccc cggtaattgc acgtccggtg gcatccgcaa
    45241 tgtgtaagaa aggcagagtg caacgtgact caatggcatc cgccacttta tgcatcgtat
    45301 tggtacataa cacaatacct tctgcgcccg cccgctgtaa gccaagcgcc gcctcagcca
    45361 gaatgtcccc ggttttatcc cattccccgc gacgctggca ctcttctatt tcatgaaaat
    45421 cgacgctatg tagcagcatt tgcgcagagt gaagcccacc aagccgctgt ttaatgcctt
    45481 catttatcag acgatagtaa ggaatggtgg attcccagct cattcctccc agcaaaccaa
    45541 ttgttttcat tcctctctcc tgatgtgtgt taccgcaaca aggctgaata aggaacgaga
    45601 caaacgcctc aacggccagg tgcccaatct ctattaacga aaaaagggcc ggatgtacag
    45661 cacatccggc ccgtgaaatc agacgccgat atttctcaac ttctcgcctg ccatcagttt
    45721 gcgttcgata tgttccagcg tgacattttt ggtttccgga atgagccaga aagtaatacc
    45781 cacaaacgca atgttcagcg cagtgtagag ccagaacgta ccggcagcgc caatgctatc
    45841 aagcagtgtc aggaaggccg cgccgataat catattcgac acccagtttg tcgtcgtcga
    45901 acaggtaata ccgaaatcgc ggcatttcag cggctgaatt tcagagcaca ggatccacac
    45961 cactggcgcg gcgctcatcg cataaccggc aatacacatc atcgtcatgc caacagagag
    46021 ccaggacaag ccactggaag ccgtaccgtt atcaaactgc atcaggcaat agcccagcac
    46081 cagagtgcct aacgccatca cgctgaaacc aattttcaga gccggtttac gacctgcttt
    46141 atctaccgta aagaccgcaa tgaaggtcgc gaacataaag gtcagtccca cgaccagagt
    46201 cgcaatcatc tgttgttctg tggtcgtaaa gcccgccatt ttgaagatac gcggcgcgta
    46261 gtacatgatg atgttcatac cggtaaactg ctgcatcgcc tgcaacaaca taccgagaaa
    46321 cacagcacga cggacgttac ggttgatctt aaacagtgcc caaccgccct gttttaactt
    46381 caggctttca cgaatttcgt tgagttcttc tcgcgctttt tccgacgtat cgcgcagcat
    46441 acgcaacact tcttccgcct caatatgacg ccccttttcc gccagccagc gcgggctatt
    46501 tggcaggaag accaccagaa taatcagcag aactgctggt aaagcaagaa cccccaacat
    46561 tgcgcgccag ttaccgctat aactgaacgc tgtatcggat aaaaacgcca gcacgatgcc
    46621 gagtgtgacc atcaactggt acatactgat catcttaccg cgaacgtttt cacttgccat
    46681 ttcagaaaga tacagaggag cggtgtaaga cgcgatcccg acagcaatgc ccagcaccac
    46741 acgagcggcg attaacatct ctacgctggt cgcaaaagcg gaccctatag aaccgagtac
    46801 aaacaggatg gcccccgcca tcaggctgta tttacgcccc aggcggaacg acagccaacc
    46861 attaaacagc gcaccaattg ctgcgccgag catcatgcta ctaaccaccc attcctgcaa
    46921 acgactggtc agcacaaagt gatcggtaat gaacggcaac gctccggcga ttacgccgat
    46981 atcaagacca aataacaatc ctgcgaccgc agcagctacc gaaacaaaca tattcatacg
    47041 ccgcgtatca cgcaaagaac gtggcgttaa agcagattcc gtattgatag taaccatttt
    47101 ttcctgccag cagagagtaa gacattgtga aaaaatacgt gaacaattca cacaggtgtc
    47161 aggtcggaaa cagcataaat atggattaaa ttgctgcgac atgccgttat gtgatggata
    47221 ttccaatttt aaaattaagt tgaattattg agattattat taacctccta attttaaagc
    47281 agataaaatt cataaagttc attaattgat aattgatatg gattatttca taaccatgat
    47341 atggattatg atgatctaga gatataaaaa accctgccat gcggcagggt cataaaagta
    47401 agaggaatga attaacgcgc cagccaaccg ccatccacag caatggcata accatttaca
    47461 taatctgaag cgctggaggc aagaaacact accggcccca tcaggtcact cggcagtccc
    47521 caacggccag ctggaatgcg atcgagaatt tccgcgctac gttgttcatc tgcccgcagt
    47581 tgttgggtat tgttggtcgc catgtaaccc ggagctatcg cattaacatt aatgttgtgt
    47641 ttagcccatt cgtttgccat caatcgcgtc acacccatca cgccgctttt tgatgcggta
    47701 taagaaggca cacggatccc gccctggaag gagagcattg acgcgatatt gataatcttg
    47761 ccgccattgc cttgcgcgat aaagtgtttc gccgctgcct gagacatgaa gaatacgctc
    47821 ttgatattca ggttcatgac atcgtcccag tctttttcgc tgaactcgag agcgtcttcg
    47881 cggcgaatca atccggcgtt attcaccagg atatcaatat gaccaaattc agctaccgcg
    47941 cgatccagca gtccaggaat gccatcaatc tttcgcagat cggcggtcag gcttaaaaaa
    48001 cgacgcccca gcgccgtgac ctgctcgatg gtttcagtcg gttcaacgat gttaatgcca
    48061 acaatgtcac agcccgcttg cgccagcccc aacgccatcc cctggcccag cccagtatca
    48121 caaccagtga cgaccgcaac tttaccttgg agagaaaatg cacttaaaat cataacaaca
    48181 ccttattcgt ttatgcccac aactagcgca aatctttaac gcccacatgg tccatatcat
    48241 taaagacctg gttttcaccg accatgcccc agataaaggt ataagctttg gttccgacgc
    48301 cggaatggat cgaccagctc ggggagatca ccgcctgctc gttatgcatc acaatgtgac
    48361 gcgtttcttg cggctgcccc atcatgtgga aaacgcaggc gtcatcatcc atattgaaat
    48421 agaaataaac ttccatccgg cgctcgtggg tgtgacacgg catggtgttc cacaagttac
    48481 ctggagccag ctccgtcagc cccatactca attgacaggt ttccagtaca tccgggacaa
    48541 aatatttgtt aatcgtgcga cggttactgg tgaggttatc gcctaacgtg actggagata
    48601 cttcgtccgg tgtgactttt ttggtgggat acgtcgtatg cgcgggtgcg caattgtaat
    48661 aaaacttcgc cggagtggcg gtatcaatac tggcaaagac aacttctttt gcacctttac
    48721 caacatacag ggcgtcgcgg tgaccgattt catagcattg accatcgaca gtaatcgtac
    48781 cggcaccgcc aatattgata acacctaact cgcgacgttc aaggaaatag cttacgccga
    48841 gttgtttacc aacttccccg ccaacggaaa ccgttttagt tatcggcata atgccgccaa
    48901 caataattcg gtcaatgtgg ctgtaaacca tggtgtactc atcggcgaca aatacctttt
    48961 caaccaaaaa ttcattgcgc agcccttggg tatccagcgt ttttgcgtgc gcaccgtgga
    49021 tgctctgtct tacgtccaca tcaacctccg ataatcagtg aagcgtgaaa atagaaaacg
    49081 atagtgaaac actgtttcgt tttctctata aagggatgat atgcccatgg gctagggttt
    49141 tcaattaaaa ataaaacaat gttttatttt taatttaagg attaaaaaaa gtgcagtgtc
    49201 gatcacgaat atgtcatgag aatggaaaaa tgagggagcc cagaaaatgt atgtcgactt
    49261 gccagccatc attattcaac agattaatga cggcagatat ttatcggtaa ggaggaaatg
    49321 cagagctact cgtcacgttc aatggtcaat gccacgccct ggcccccgcc gatacaaagc
    49381 gttgccagcc ctttgcgggc attacgtttc accatttcat gaaccagaga aaccaggatt
    49441 cggcaaccgg aagcgcctat cgggtgaccg agtgcgatcg cgccaccatt gacattgacc
    49501 cgacgctcat cccactcaag catcttgcca accgaaagcg cctgtgcagc aaacgcttca
    49561 ttagcctcga taagatcgac atcagccaac tgccagccta cacgctccag gcaacggcgg
    49621 gtcgcataca ccggcgcaat tcccatcaat gccggatcta caccaacgct ggcaaatgcg
    49681 cggatgcggg ccagcacggg taaattcaac gctcgtgctt tggcttcgct catcatcatt
    49741 accgcagctg cgccatcgtt tatggatgat gcattccccg cggtcacaga accgagacta
    49801 tcaaatgaag gatttaaacg ggctaaacct tctgcactgg cgtcagtgcg tggctgttca
    49861 tcggtatcaa caaccaacgt ctgaccgtta ctttggatca ttaccgggac gatctcatct
    49921 ttaaatcgtc cggcgtcaat cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca
    49981 tcctgcaact gacggctgat gccatattcg cgagccagat tttcggcggt gacaccaata
    50041 tgataatcat tgaaggcatc ccacaaccca tcatgcacca gactgtcaac caactggcta
    50101 ttgccaagct gtgcaccggt gcggctatca gtcagaacat gtggtgcgcg gctcatgttt
    50161 tcctggccac cggcgatgac aatatcagcc tcgccacact gtatcgcctg agtagccaga
    50221 tgcagtgctt taagaccgga accgcaaacg tcattaatcg tgattgcaga aacgctgtta
    50281 ggaagaccac ctttaatagc cgattgcctt gccggattct gccctgcccc tgcagtcaac
    50341 acctgaccaa gaattacttc atccaccgca tatgcaggaa cgccggtacg ttctattaac
    50401 gctttcacga ccagactacc aagttccacg gcggaatgac ccgctaacgc accacgaaag
    50461 cagccgatag gtgtccgtaa cgccccgaca atcacaacgt ctttcatcac aacctcgcac
    50521 caattaacag tgcaatagta aatgattgtt aattacagtc atcttaattg tttaaaaaaa
    50581 gtgattttta tcacaaagga aatatgcctg agcagtagtc agagtcataa ctggcacgta
    50641 aggtttgcaa ccactaaccc accaatagat gggtagatag ggcgttaatc tcccatactt
    50701 aacttggttt atggtaaatt gccctccatt ttgtttaatt tgtagatgat acgttcagat
    50761 aatgtctaat atttggtcta aagaagaaac tctgtgaagt ttcgcgctct atggcacagc
    50821 cgttgatgca ggcacgctat tccttcctat tcagttaggt tcggcagggg ctgtggtcct
    50881 gtttattact gatctggtcg cctggccttt aacatattgg ccacataaag ccttatgcca
    50941 gttcatcctc tcatcaaaaa catcagcagg tgaagggata acgggcgcgg taacacacta
    51001 ctatggcaag aagattggta atctgattac cacgctgtac ttcatcgcct tttttgtcgt
    51061 cgtgttgata tatgcagtgg caattaccaa ctcacttacg gaacagctgg caaagcatat
    51121 ggttattgat cttcgcatcc gtatgttggt gagtctgggt gttgtattaa ttctgaatct
    51181 catttttctg atgggacgtc atgccactat tcgggtaatg ggatttttgg tattcccatt
    51241 gattgcctat ttcttatttc tttccattta ccttgtcggt agttggcaac ctgatctatt
    51301 aacaacccag gtagagttca atcagaatac ccttcaccag atatggatat cgattcccgt
    51361 gatggttttc gcctttagcc atacgcccat tatttctacg tttgccatag acagacgtga
    51421 aaaatatggc gaacacgcta tggataaatg caaaaaaatt atgaaagtcg cttatctcat
    51481 catctgcata agtgtactgt tctttgtctt tagctgcctg ctttctattc catcttcgta
    51541 tattgaagcg gctaaagaag aaggggtcac cattttatcg gcgctttcta tgctgccgaa
    51601 cgccccagca tggttgtcaa tttccgggat tattgtcgca gtagttgcga tgtcgaaatc
    51661 attcctgggt acgtactttg gcgttattga aggtgccaca gaggtcgtca aaacaacact
    51721 acagcaggtt ggtgtaaaga aaagtcgtgc atttaaccac gcactatcaa ttatgttggt
    51781 atcgctgatt accttcattg tttgttgcat taacccgaac gcgatttcga tgatttacgc
    51841 gatcagcggc ccgctcattg ccatgatact tttcatcatg cctacgctgt caacgtatct
    51901 catcccggcg cttaaaccct ggcgttccat cggaaatctg attacgctga tcgtgggtat
    51961 cctgtgcgta tcggtaatgt tctttagcta atatccgttg tggatggttc aaaattgcta
    52021 caattttgaa ccattccatt tttcataata ttcatttgtt attattccgc cctatcactt
    52081 ctctcgtcat atcctagaat tatcagttag ttaaacacac aaagagatct gaacatctta
    52141 aataagacta tttaagatgc ataacttaga ttcgcaagat atcttctggt cattcaaaaa
    52201 caatttgcga tatagatatt ctcataccat gagatagtta tctggaccac gttcgaccat
    52261 gcaagattaa atcagtgaaa cataatcata tttaataacg agaaatgcat ttttaaatat
    52321 cttaatcaga attatagcaa atacagatta atcgcaattt ttcccagcaa aattacaaaa
    52381 tcaaggtgta ttgacatcat caatcaccaa tgaaattatg caattatata cggataggga
    52441 ggttcttaac atggggcttt gtagtcgtta taaaagtctt acatgcaata gttgctcaat
    52501 gcattgccaa ataatgccag aagagtcacc gcgtttgcag tactgcgcaa actcgtgtt
//
LOCUS       DAJTRB010000018        49779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTRB010000018 DAJTRB010000000
VERSION     DAJTRB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.018"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..1173
                     /locus_tag="OO330_001135"
     CDS             <1..1173
                     /locus_tag="OO330_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5882079.1"
                     /translation="DSVCLSTWKAALLGSEHKRVPDDLENHPVITGHECAGVIVEVGK
                     NLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGS
                     YFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYA
                     INGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALT
                     GDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVPFNFYNVHYNSTHV
                     VGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNG
                     VTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1170..2135
                     /gene="yggF"
                     /locus_tag="OO330_001136"
     CDS             1170..2135
                     /gene="yggF"
                     /locus_tag="OO330_001136"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5882080.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2157..2666
                     /gene="fumE"
                     /locus_tag="OO330_001137"
     CDS             2157..2666
                     /gene="fumE"
                     /locus_tag="OO330_001137"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5882081.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2663..3376
                     /locus_tag="OO330_001138"
     CDS             2663..3376
                     /locus_tag="OO330_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5882082.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3348..4025)
                     /locus_tag="OO330_001139"
     CDS             complement(3348..4025)
                     /locus_tag="OO330_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5882083.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4019..4696)
                     /locus_tag="OO330_001140"
     CDS             complement(4019..4696)
                     /locus_tag="OO330_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5882084.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4684..5067)
                     /locus_tag="OO330_001141"
     CDS             complement(4684..5067)
                     /locus_tag="OO330_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5882085.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5083..5391)
                     /locus_tag="OO330_001142"
     CDS             complement(5083..5391)
                     /locus_tag="OO330_001142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882086.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5392..5970)
                     /locus_tag="OO330_001143"
     CDS             complement(5392..5970)
                     /locus_tag="OO330_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5882087.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5993..6424)
                     /locus_tag="OO330_001144"
                     /pseudo
     CDS             complement(5993..6424)
                     /locus_tag="OO330_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6796..7815
                     /gene="epd"
                     /locus_tag="OO330_001145"
     CDS             6796..7815
                     /gene="epd"
                     /locus_tag="OO330_001145"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5882088.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLFLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7865..9028
                     /gene="pgk"
                     /locus_tag="OO330_001146"
     CDS             7865..9028
                     /gene="pgk"
                     /locus_tag="OO330_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5882089.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9243..10322
                     /gene="fbaA"
                     /locus_tag="OO330_001147"
     CDS             9243..10322
                     /gene="fbaA"
                     /locus_tag="OO330_001147"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5882090.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10513..11373
                     /gene="mscS"
                     /locus_tag="OO330_001148"
     CDS             10513..11373
                     /gene="mscS"
                     /locus_tag="OO330_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5882091.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11512..12147
                     /gene="argO"
                     /locus_tag="OO330_001149"
     CDS             11512..12147
                     /gene="argO"
                     /locus_tag="OO330_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5882092.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12240..12980
                     /locus_tag="OO330_001150"
     CDS             12240..12980
                     /locus_tag="OO330_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5882093.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13147..14097
                     /locus_tag="OO330_001151"
     CDS             13147..14097
                     /locus_tag="OO330_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5882094.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14039..15517)
                     /gene="scpC"
                     /locus_tag="OO330_001152"
     CDS             complement(14039..15517)
                     /gene="scpC"
                     /locus_tag="OO330_001152"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR5882095.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15541..16326)
                     /gene="scpB"
                     /locus_tag="OO330_001153"
     CDS             complement(15541..16326)
                     /gene="scpB"
                     /locus_tag="OO330_001153"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5882096.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16337..17332)
                     /gene="meaB"
                     /locus_tag="OO330_001154"
     CDS             complement(16337..17332)
                     /gene="meaB"
                     /locus_tag="OO330_001154"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5882097.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17325..19469)
                     /gene="scpA"
                     /locus_tag="OO330_001155"
     CDS             complement(17325..19469)
                     /gene="scpA"
                     /locus_tag="OO330_001155"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5882098.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19673..20566)
                     /gene="argP"
                     /locus_tag="OO330_001156"
     CDS             complement(19673..20566)
                     /gene="argP"
                     /locus_tag="OO330_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5882099.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20949..21608
                     /gene="rpiA"
                     /locus_tag="OO330_001157"
     CDS             20949..21608
                     /gene="rpiA"
                     /locus_tag="OO330_001157"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5882100.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21864..23096
                     /gene="serA"
                     /locus_tag="OO330_001158"
     CDS             21864..23096
                     /gene="serA"
                     /locus_tag="OO330_001158"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5882101.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23325..23384
                     /gene="ibsC"
                     /locus_tag="OO330_001159"
     CDS             23325..23384
                     /gene="ibsC"
                     /locus_tag="OO330_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5882102.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23717..23776
                     /locus_tag="OO330_001160"
     CDS             23717..23776
                     /locus_tag="OO330_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5882103.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23877..24479)
                     /locus_tag="OO330_001161"
     CDS             complement(23877..24479)
                     /locus_tag="OO330_001161"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5882104.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRASKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24500..24683)
                     /gene="ssrS"
                     /locus_tag="OO330_001162"
     ncRNA           complement(24500..24683)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO330_001162"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24725..25054)
                     /gene="zapA"
                     /locus_tag="OO330_001163"
     CDS             complement(24725..25054)
                     /gene="zapA"
                     /locus_tag="OO330_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5882105.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25222..25800
                     /gene="ygfB"
                     /locus_tag="OO330_001164"
     CDS             25222..25800
                     /gene="ygfB"
                     /locus_tag="OO330_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5882106.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25826..27151
                     /gene="pepP"
                     /locus_tag="OO330_001165"
     CDS             25826..27151
                     /gene="pepP"
                     /locus_tag="OO330_001165"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5882107.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27148..28326
                     /gene="ubiH"
                     /locus_tag="OO330_001166"
                     /gene_synonym="visB"
     CDS             27148..28326
                     /gene="ubiH"
                     /locus_tag="OO330_001166"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5882108.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28349..29551
                     /gene="ubiI"
                     /locus_tag="OO330_001167"
                     /gene_synonym="visC"
     CDS             28349..29551
                     /gene="ubiI"
                     /locus_tag="OO330_001167"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5882109.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29625..29708)
                     /locus_tag="OO330_001168"
     CDS             complement(29625..29708)
                     /locus_tag="OO330_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882110.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29999..31093
                     /gene="gcvT"
                     /locus_tag="OO330_001169"
     CDS             29999..31093
                     /gene="gcvT"
                     /locus_tag="OO330_001169"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5882111.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTWEFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31117..31506
                     /gene="gcvH"
                     /locus_tag="OO330_001170"
     CDS             31117..31506
                     /gene="gcvH"
                     /locus_tag="OO330_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5882112.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31625..34498
                     /gene="gcvP"
                     /locus_tag="OO330_001171"
     CDS             31625..34498
                     /gene="gcvP"
                     /locus_tag="OO330_001171"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5882113.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34765..35508
                     /locus_tag="OO330_001172"
     CDS             34765..35508
                     /locus_tag="OO330_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5882114.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35565..36998)
                     /gene="bglA"
                     /locus_tag="OO330_001173"
     CDS             complement(35565..36998)
                     /gene="bglA"
                     /locus_tag="OO330_001173"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5882115.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37043..37353
                     /locus_tag="OO330_001174"
                     /pseudo
     CDS             37043..37353
                     /locus_tag="OO330_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37517..38176
                     /locus_tag="OO330_001175"
     CDS             37517..38176
                     /locus_tag="OO330_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5882116.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38372..39352)
                     /gene="ygfZ"
                     /locus_tag="OO330_001176"
     CDS             complement(38372..39352)
                     /gene="ygfZ"
                     /locus_tag="OO330_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5882117.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39595..39861
                     /gene="sdhE"
                     /locus_tag="OO330_001177"
     CDS             39595..39861
                     /gene="sdhE"
                     /locus_tag="OO330_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5882118.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39842..40249
                     /locus_tag="OO330_001178"
     CDS             39842..40249
                     /locus_tag="OO330_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5882119.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40289..40810)
                     /gene="fldB"
                     /locus_tag="OO330_001179"
     CDS             complement(40289..40810)
                     /gene="fldB"
                     /locus_tag="OO330_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5882120.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40922..41818
                     /gene="xerD"
                     /locus_tag="OO330_001180"
     CDS             40922..41818
                     /gene="xerD"
                     /locus_tag="OO330_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5882121.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41843..42553
                     /gene="dsbC"
                     /locus_tag="OO330_001181"
     CDS             41843..42553
                     /gene="dsbC"
                     /locus_tag="OO330_001181"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5882122.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42559..44292
                     /gene="recJ"
                     /locus_tag="OO330_001182"
     CDS             42559..44292
                     /gene="recJ"
                     /locus_tag="OO330_001182"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5882123.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLLGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44383..45481
                     /gene="prfB"
                     /locus_tag="OO330_001183"
     CDS             join(44383..44457,44459..45481)
                     /gene="prfB"
                     /locus_tag="OO330_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5882124.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45491..47008
                     /gene="lysS"
                     /locus_tag="OO330_001184"
     CDS             45491..47008
                     /gene="lysS"
                     /locus_tag="OO330_001184"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5882125.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47051..47599)
                     /gene="idi"
                     /locus_tag="OO330_001185"
     CDS             complement(47051..47599)
                     /gene="idi"
                     /locus_tag="OO330_001185"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5882126.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47654..47725
                     /locus_tag="OO330_001186"
     CDS             47654..47725
                     /locus_tag="OO330_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882127.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47722..47847)
                     /locus_tag="OO330_001187"
     CDS             complement(47722..47847)
                     /locus_tag="OO330_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882128.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47849..49297)
                     /gene="uacT"
                     /locus_tag="OO330_001188"
     CDS             complement(47849..49297)
                     /gene="uacT"
                     /locus_tag="OO330_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5882129.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12034 a        12449 c        13392 g        11904 t
ORIGIN      
        1 gacagcgtct gtttatcgac ctggaaagcg gcgttactcg gtagtgaaca taaacgcgta
       61 cccgacgatt tagaaaatca tccggtcatt accgggcatg aatgtgccgg ggttattgtc
      121 gaagtgggta aaaatctcac tggcaaatat aaaaaaggcc agcgttttgt attgcaaccg
      181 gcgatggggt taccaagcgg atattcagcg ggctacagct acgaatattt tggcggcaat
      241 gccacttata tgattattcc cgaaatcgcc attaatttgg gctgcgtatt accgtatcac
      301 ggctcttatt ttgctgcggc gtcgctggca gagcctatgt gctgcattat tggtgcttat
      361 catgccaatt atcacaccac gcaatatgtt tatgagcatc gcatgggcgt caaacctggc
      421 ggcaatattg cactgctggc gtgtgcgggt ccgatgggca ttggcgctat cgattacgcc
      481 attaacggtg gcatacaacc gtcacgggtg gtggtggtcg atatcgacga caaacgcctg
      541 gcgcaggtgc agaagctgct gccggtggat ctggcggcca gtaaaggcat tgagctggtg
      601 tatgtgaata ccaaagggat gagcgatcct atccagatgc tgcgggcgct gacaggagat
      661 gtcgggttcg atgacatttt tgtttatgcg gcggtgcctg ctgtcgttga gatggctgac
      721 gaattactgg cggaagatgg ctgtctgaac ttctttgccg ggctgacgga taaaaacttc
      781 aaagtgccgt ttaatttcta caacgtccat tacaacagca cgcacgtagt cggtacatcc
      841 ggcggttcaa cggacgacat gaaagaggcg attgccctta gcgccacagg acagttacag
      901 ccgtcgttta tggtgaccca tattggtggc ctggatgcgg tgccagaaac cgtgctcaat
      961 ctgccggata tccctggcgg taaaaaactc atttataacg gcgtgaccat gccgctcact
     1021 gccattgccg attttgccga aaaaggcaaa accgatccgc tgtttaaaga gttggcgcgg
     1081 ctggttgagg aaacgcacgg catctggaat gaacaggccg agaaatatct gctggcacaa
     1141 tttggcgttg atatcgggga ggccgcgcaa tgatgtccct ggcgtggcca ttatttcgcg
     1201 ttacggaaca ggcagcgttg gctgcctggc cgcaaaccgg atgtggcgac aaaaataaaa
     1261 ttgatggcct ggcggtcacc gcgatgcgcc aggcattaaa cgacgtcgct tttcgtggac
     1321 gagtggttat cggcgaagga gaaattgacc atgcgccgat gctgtggatt ggcgaagagg
     1381 tgggcaaagg cgatgggcca gaagtcgata tcgcggttga tcccattgaa ggcacgcgga
     1441 tggtggcgat ggggcagagc aatgcgctgg cggtcatggc tttcgctcct cgagatagcc
     1501 tgttacatgc gcccgatatg tatatgaaaa agctggttgt taatcgactg gctgccgggg
     1561 cgattgatct gtctttgcct ctggcggaca acttgcgtaa cgtggcgcgg gcgttaggta
     1621 aaccgctgga caagctgcgc atggttacgc tggataaacc gcgtctgagt gccgcgattg
     1681 aagaagcaac acagctgggc gtgaaggttt ttgccctgcc ggatggcgat gtcgccgcca
     1741 gcgtgctgac ctgctggcag gataatcctt acgatgtgat gtacaccatc ggcggtgcgc
     1801 cagaaggcgt gatttctgcc tgtgccgtta aagcgttagg cggggatatg caggccgagc
     1861 tgattgactt ttgtcaggct aaaggggatt acacggaaaa tcggcagatt gcagagcagg
     1921 agcgcaagcg ttgtaaggca atgggcgttg acgttaaccg tgtgtactcg ctcgatgaac
     1981 tggtgagggg gaacgatatc ctctttagcg ctacgggcgt gacgggcggc gagttggtga
     2041 acggtatcca acagacggcg aatggggtgc ggacgcagac attactgatc ggcggcgcgg
     2101 accaaacgtg taatataata gactccctgc attgatggtg atctatcgga agaccaatgg
     2161 cgacgctgac agaagatgat gtgcttgagc aactggatgc acaggacaat ttattttcat
     2221 ttatgaaaac tgcgcattct attttgctcc aggggatacg ccagtttctg ccgtcgctgt
     2281 ttgtcgataa cgatgaagag atcgtcgaat atgcagtgaa gccgttactc gcccaaagcg
     2341 gcccgcttga cgatattgat gttgcgctgc gtttgattta tgcgctgggg aaaatggata
     2401 aatggctgta tgccgatatc acgcactttt cccagtactg gcattacctg aacgaacagg
     2461 atgaaacgcc cggatttgcc gatgatataa cctgggattt tatcagcaat gtcaacagta
     2521 taactcgcaa tgcgacgctc tacgatgcgt taaaagcgat gaagttcgcc gatttcgccg
     2581 tctggtcaga ggcgcgtttt agcggaatgg tcaaaacggc gctgacgctg gcagtaacga
     2641 ccaccttaaa ggaattaacg ccgtgaaaat tgaattaacg gtgaatgggc tgaacgtgca
     2701 agcgcagtac catgatgaag agatagaacg cgtgcataaa ctgttgctgc gtatgctggc
     2761 ggccttacag acggtaaatc cgcagcggcg tacggtggtt tttctttgcg ctccgccggg
     2821 gacgggtaaa tctaccctaa ccaccttctg ggaatatctc gcgcaacaag acccggagct
     2881 gcccgctatt caggcgctcc cgatggatgg ttttcaccat tacaatagct ggctggacgc
     2941 gcatcaattg cgccccttca aaggcgcacc agagacattt gacgttgcga aactggcgga
     3001 aaatctgcgc caggtggtgg aaggggattg tacgtggccg cagtacgatc gacaaaagca
     3061 tgatcctgtt gaagatgcgt tgcacgttac cgcaccactc gtcatcgtcg aaggaaactg
     3121 gttgttgctg gacgacgaga agtggtgcca gcttgcgcaa ttttgtgatt tctcgatctt
     3181 tatcaacgcg ccagctgctg cgttgcggga gcgtctggtg gggcgcaagc tggcgggagg
     3241 ggtatcgctg gcagatgcag aagcctttta tgaccgtacc gacgggccga atgttcgccg
     3301 ggtgctggaa gagagtctgc ctgcaaattt gaccttaatg atgaccgcta cgggtgaata
     3361 tcgtctggtg gattaacgtt cctgattaca ccatcttcca gccgcaccac acgagagaaa
     3421 tgacgccggg taaatgcggc gtcatgactg attgctacca cgctcgtgcc tcgctggcgg
     3481 cacttctcca gccagctttc aaacacgctc aaccaatttt cgtcaaaatc ccgactcggc
     3541 tcgtccagca gtaatagtgg cggagagagt gcttccaggc aggcgacggc gaccatccgt
     3601 cgctgcgcac tatgcagatc caacgggtgc gagttggcag tgtctgctaa ctgacaacat
     3661 tgcagggcag catgggtacg ttgagtgatt tcatccgccg ggcatttttg cagtttcaaa
     3721 ccaaatgcga tctcgtcagc gacggtgctg tgaaaaagtt ggttttccgc ttcctgaaac
     3781 agtacgccaa cctttgccgc gcgctggcgg tttttcaggt ttttcatcgc ctgttgttgc
     3841 agcataaccg taccggctgt gggcgtaaga agacctgcca ttactcgtaa cagcgtcgat
     3901 ttccctgcgc cattgtcacc ggtcagcgcc aaccattcac cttgtttgag ttgcagagaa
     3961 atgtcgcaaa gacaatccgt tgccgcgcca ggccagcgat aagatatctt atttaacgtt
     4021 aacatagtgg ctggagcgct ccgttttgca attgccacgc ttgctggcag tagttcagga
     4081 aaggtgtgcg atgacgttca aaaagaataa tcagcgaacc acgttcaaac gcccagtgtt
     4141 gtaagcgctg caacagcatt tcgctggcct gcggcgtcag gcggctaaag gcttcatcaa
     4201 gaattaagag ctttggctgc atcgcgatgg cgcaggcgat gatcacgcgc tgtgtctctc
     4261 caccggagag tgttgccggg tgacgatggc gcaacggctg gcactcggtc agagtcaggg
     4321 cggcatcaat ccgcgccata atctctgctt cagcaagaca cagattttct ggcccaaagg
     4381 caacttcttc ttccacgcta aaggtacagc cggagagctg caaatagggc gattgctgga
     4441 cgagctggat ggtggcggat tgttcattaa gcgggagatg cccaatgggc gtaccgagga
     4501 gcgtccccgt gcctgttatc tctcttggca gaaagtccgg ataccagccc gccatcagct
     4561 gtgccagcgt acttttgcca ctgccgttgt caccaaagat ggcgaccatc cccggtttga
     4621 catagtgaaa gtcataacag aacggaggat gcgttgagtg tgtcgggcaa taacggaact
     4681 gttctaacgt aaccatatcc acgctccgaa ttctgtcagc atcagcaaga tcatggtgta
     4741 acgcgctacg cgttgtaggg tgctatcggc tggtgcccac aacgttgttc gtcggttgtg
     4801 gatccgaaat gcccgcatat ccagcgccgc accacggaca gccagatcgt taagcgcatt
     4861 gtgtgttagc ggaataatta acgcgggcat agcacgtaaa cgctgatacc agccctcatc
     4921 cagcggcacg ccgcgggcac gctgcgcttc atgaataatc gctaattgcc gttttaactg
     4981 ctcaacgacc aataacggcc cggcaaacag ataagcgacg cctggcggca ggcgagaagc
     5041 aaacagagcg cgaataaaac attgcacggg gacgtactgc attcacaact gtgaggttga
     5101 aacaatcgcc agaatcagca accagagcgt aatagcatgc gaccaccgtt ctggcgagcg
     5161 tggcgtgccg cttagccatt ctggtagcca gccaccgtgt accagccaca gcccggctcc
     5221 cagcgagaac atcagccaga cgacatattt tgcccgccgc cgcgtggcct ttagcgcaat
     5281 aaggcagaaa aaggtcgccg cgctatagat cggcaaaatg gtctgcgcgg gcaggataag
     5341 cgtggtgcag gccgccaatg cccacagtgt taatgaggta aatggatgca tttagcgcac
     5401 ggcagccatt gccggaaaat ggcgcgtggt gcgaatcggc agctggcgca gtaaaagcca
     5461 gacaatcacc gctgtaagaa ttttatcgac caggttcgcg cctatcaccg taatcgccac
     5521 cgactccacc aggttttgcc ccatcgagtg catccaggca acgaacaaat cggctccgct
     5581 accggtgaca ccgccgaaca gcgcagtacg cagcggtaca gcgacgaccg ttaccgcaag
     5641 cgtaataatc acgccgctga caacgacttt tggcagggtg cgaaaccaac ccgctcgcgc
     5701 cagccaaccg gagaccaggc cgatgaccat cgcgacgggt gcaaatgcgg cggcgatcgg
     5761 gtcggtcagc aatccccaga gtaaattggt cagtaagccg gtcagcattc cgatcaccgg
     5821 accaagcagt accgcgctaa taagtgtgcc gatggagtcg agaaatatag gtaatttgac
     5881 catgctggcc agttgcccgc cgatcatatt gatggcgata gatataacga tcaacaccag
     5941 tgcctgactg gaaaagtggc tacgcgccat aagaaaacct ctttgaaagt agttatttaa
     6001 cgggggaaat atgcaactcg tcgtaaccag aaagcgcgca aggttggcgg ctaaacgcca
     6061 gttcctcaag gctaccgatc accagttcaa gcgtggtccg cacggtacag ccgggcatca
     6121 tatgaccgcc aagcatcgtg ccgtgtggat cggaaacgca gagatgaaga tgctcaccac
     6181 tctgttccag cgtgccgttt agtgagatca cttcgaatgt accgttcagg agcgtggtgc
     6241 cttcttgccc ggcatagcgc aaggcgacgt cagtcaggct accggtacag cctgcaatct
     6301 aggcggcatg aagttgatgt tgctgtgcaa aagcgtgtag ctgggaaaaa acttcctgcc
     6361 cggggaggag gcgcagtgcg taaaagcgtg cggaggaaga atatgagtag ggcaatgtca
     6421 tcattggagt actccgaaac tcggacgtat tgcggataat atcgacgctg catcagtcat
     6481 attctgatat aagtcagggt ttgtgacggc acaacgaaaa gattttgcta cgcttattcg
     6541 tgattctgca aagtggaata aattccacag gggcggggct gaaaacgtga cgtaagtcag
     6601 ataattgctt tctttgactg gacaatcatt ccttttattc cacgtttcgc ttatcctagc
     6661 tgaagcgttt cagtcgatta aatgttcgac aattaaccaa tcagtcgcag tttgcgacag
     6721 gtaaggtttc cccggacgat ttgctggatt actctgtctg gcaaatttgc tcagggaaaa
     6781 ccttgcagga gatctatgac cgtacgcgta gcgataaatg gcttcggtcg catcgggcgt
     6841 aatgtggttc gtgctttgta tgaatccgga cgccgggcgg aaattaccgt ggtggcaatc
     6901 aacgaactgg cggatgctgc gggcatggcg catttgttga aatatgacac cagccatggc
     6961 cgttttgcat gggaagtacg acaggaacgc gatcaacttt ttgttggtga tgacgccatc
     7021 cgcgtattgc atgaacgttc actgcaatcg ctcccctggc gtgaacttgg cgttgatgta
     7081 gtcctcgact gcaccggcgt atatggctcc cgcgagcatg gcgaagcgca tattgccgcc
     7141 ggggccaaaa aagtgctctt ttcacatcct ggcagtaacg atctcgacgc gaccgttgtt
     7201 tacggcgtca atcaggatca acttcgtgcg gaacaccgca tcgtttctaa cgcttcctgt
     7261 accacgaatt gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg tattgagtcc
     7321 ggcactgtga ccacaattca ctccgccatg cacgatcaac aggttattga tgcataccat
     7381 cctgacctgc gtcgcacccg ggcagccagc cagtcgatca ttccggtcga tactaaactg
     7441 gccgctggta tcacacgatt ttttccgcaa tttaacgatc gctttgaagc gattgcggta
     7501 cgtgtgccaa ccataaatgt gacggcaatc gatttaagcg tgacggtgaa gaaacctgta
     7561 aaagccaatg aagtcaacct gtttctgcaa aaagcagcac aaggtgcatt tcatggtata
     7621 gttgactata cggaattgcc gttggtctct gtagatttta accacgatcc gcacagtgcc
     7681 attgtcgatg gcacccaaac ccgggtcagt ggcgcacacc tgatcaaaac gttggtctgg
     7741 tgcgataacg aatggggctt tgctaaccga atgctcgaca cgacgttagc tatggctact
     7801 gttgctttca ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa cgagaggatt
     7861 caccatgtct gtaattaaga tgaccgatct ggatcttgct gggaaacgtg tatttatccg
     7921 tgcggatctg aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc gtatccgtgc
     7981 ttctctgccg accatcgaac tggccctgaa acaaggcgca aaagtgatgg taacttccca
     8041 cctgggtcgt cctaccgaag gcgagtacaa cgaagaattc tctctgctgc cggttgttaa
     8101 ctacctgaaa gacaaactgt ctaacccggt tcgtctggtt aaagattacc tcgacggcgt
     8161 tgacgttgct gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca aaggcgagaa
     8221 gaaagacgac gaaaccctgt ccaaaaaata cgctgcactg tgtgacgtgt tcgtaatgga
     8281 cgcattcggt actgctcacc gcgcgcaggc ttctactcac ggtatcggta aattcgctga
     8341 cgttgcgtgc gcaggcccgc tgctggcagc tgaactggac gcgctgggta aagcactgaa
     8401 agaacctgct cgcccgatgg tggctatcgt tggtggttct aaagtatcta ccaaactgac
     8461 cgttctggac tccctgtcta aaatcgctga ccagctgatt gttggtggtg gtatcgctaa
     8521 cacctttatc gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag ctgacctggt
     8581 tgacgaagct aaacgtctgc tgaccacctg caacatcccg gttccgtctg atgttcgcgt
     8641 agcaaccgag ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg atgtgaaagc
     8701 tgacgagcag atcctggata tcggtgatgc ttccgctcag gaactggctg aaatcctgaa
     8761 gaatgcgaaa accattctgt ggaacggtcc ggttggcgtg ttcgaattcc cgaacttccg
     8821 caaaggtact gaaatcgtgg ctaacgctat cgcagacagc gaagcgttct ccatcgctgg
     8881 cggcggcgac actctggcag caatcgacct gttcggcatt gctgacaaaa tctcctacat
     8941 ctccactggc ggcggcgcat tcctcgaatt cgtggaaggt aaagtactgc ctgcagtagc
     9001 gatgctcgaa gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc tgcacttgtt
     9061 tcagcgcgct tttagagcac gcattttttc atactctaaa taattcgagt tgcaggaagg
     9121 cgacaagaga gtgaatcccc aggagcttac ataagtaagt gactggggtg agcgaacgca
     9181 gacgcagcac atgcaacttg aagtattatg agtataaggc ccgacgatac aggacaagag
     9241 acatgtctaa gatttttgat ttcgtaaaac ctggcgtaat cactggtgat gacgtacaga
     9301 aagttttcca ggtagcaaaa gaaaacaact tcgcactgcc agcagtaaac tgcgtcggta
     9361 ctgactccat caacgccgta ctggaaactg ctgctaaagt taaagcgccg gttatcgttc
     9421 agttctccaa cggtggtgct tcctttatcg ctggtaaagg cgtgaaatct gacgttccgc
     9481 agggtgctgc tatcctgggc gcgatctctg gtgcgcatca cgttcaccag atggctgaac
     9541 attatggtgt tccggttatc ctgcacactg accactgcgc gaagaaactg ctgccgtgga
     9601 tcgacggtct gctggacgcg ggtgaaaaac acttcgcagc taccggtaag ccgctattct
     9661 cttctcacat gatcgacctg tctgaagaat ctctgcaaga gaacatcgaa atctgctcta
     9721 aatacctgga gcgtatgtcc aaaatcggca tgactctgga aatcgaactg ggttgcaccg
     9781 gtggtgaaga agacggcgtg gacaacagcc acatggacgc ttctgcactg tacacccagc
     9841 cggaagacgt tgattacgca tacaccgagc tgagcaaaat cagcccgcgt ttcaccatcg
     9901 ctgcgtcctt cggtaacgta cacggtgttt acaagccggg taacgtggtt ctgactccga
     9961 ccatcctgcg tgattctcag gaatatgttt ctaagaaaca caacctgccg cacaacagcc
    10021 tgaacttcgt attccacggt ggttccggtt ctactgctca ggaaatcaaa gactccgtaa
    10081 gctacggcgt agtgaaaatg aacatcgata ccgataccca atgggcaacc tgggaaggcg
    10141 ttctgaacta ctacaaagcg aacgaagctt atctgcaggg tcagctgggt aacccgaaag
    10201 gcgaagatca gccgaacaag aaatactacg atccgcgcgt atggctgcgt gccggtcaga
    10261 cttcgatgat cgctcgtctg gaaaaagcat tccaggaact gaacgcgatc gacgttctgt
    10321 aagtcattcc tttctgctta tctcaaggcc cgctctgcgg gtcttttttt cgccaaaagc
    10381 aaggtgattc agatgagaaa tctgtgatct atttggcaaa attatgcttt attgtttacc
    10441 cttgtcagac tgcccgtcat aaggcggcgg agtgtatttc tccattttga gtcagttgaa
    10501 aaggaatatt gaatggaaga tttgaatgtt gtcgatagca taaacggcgc gggaagctgg
    10561 ctggtagcta accaggcgct gctgctaagt tatgcagtaa acatcgtggc ggcactcgcg
    10621 atcatcatcg ttggtttgat tatcgcgcgg atgatttcca acgcggtgaa tcgcctgatg
    10681 atctcccgta aaatcgatgc cactgttgct gattttcttt ctgcattagt ccgttacggt
    10741 attatcgcct ttacgctaat cgctgccctg ggacgcgtgg gtgtacaaac agcgtcagtc
    10801 attgctgtac tcggtgccgc aggcttagct gttggtctgg ctttgcaggg gtcactttct
    10861 aacctggccg ctggcgtgtt acttgtcatg ttccgcccgt tccgtgccgg agaatatgtt
    10921 gacctcggcg gcgtagccgg tactgtgctg agtgtgcaga ttttctccac caccatgcgt
    10981 actgcagacg gtaaaattat cgttattccg aacggtaaaa ttattgccgg aaatattatt
    11041 aacttctccc gcgagccagt tcgccgtaac gaatttatta ttggcgtggc gtatgattcc
    11101 gatatcgatc aggttaagca gatcctgacc aatattatcc agtctgaaga tcgcattttg
    11161 aaagatcgcg aaatgactgt gcgcctgaac gaacttggtg catcgtcgat taatttcgtg
    11221 gtccgcgtct ggagcaacag cggcgatctg caaaacgtgt actgggatgt gctggagcgt
    11281 attaaacgtg aatttgatgc cgccggtatc agcttcccgt acccgcaaat ggatgtgaac
    11341 tttaagcggg tgaaagaaga caaagctgcg taatcaacgc tgaggccaga taatactctc
    11401 tggcctctct cttattagtt attctgattg ccaattaata ttatcaattt ccgctaataa
    11461 caatcccgcg atatagtctc tgcatcagat acttaattcg gaatatccaa cgtgttttct
    11521 tattactttc aaggtcttgc tcttggggca gctatgattc tgccgctcgg cccacaaaat
    11581 gcttttgtga tgaatcaggg cattcgtcgt cagtaccaca ttatgattgc cttactttgc
    11641 gctatcagcg atttggtcct gatttgcgcg gggatttttg gtggtagcgc gttattgatg
    11701 cagtcgccgt ggttgctagc gctggtcacc tggggcggcg tagtcttctt gctctggtat
    11761 ggttttggcg cttttaaaac agcgatgagt agtaatattg agttagctag tgccgaagtc
    11821 ctgaagcaag gcagatggaa aattatcgcc accatgttgg cagtgacctg gctgaatccg
    11881 catgtttacc tggatacttt tgttgtactg ggcagccttg gcgggcagct tgacgtcgaa
    11941 ccaaaacgtt ggtttgcgct cgggacaatt agcgcctctt tcctgtggtt ctttggtctg
    12001 gctattctcg cagcctggct ggcaccgcgc ctgcgcacgg caaaatcaca gcgcattatc
    12061 aatctggtag tgggatgtgt catgtggttt atagccttgc agctggcgag agacggtatt
    12121 gctcatgcac aagccttgtt cagttaggcg ctatctgatg gaaaaataaa acagaggcgc
    12181 taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa ttaacacgga gagactaacg
    12241 tgaagttcaa agttatcgcc ctggcggcat taatgggtat tagcgggatg gcagcgcagg
    12301 ctaacgaatt gccggatgga ccgcatattg tcacctccgg tacggcaagt gtggatgcgg
    12361 tgccagacat tgccactctt gcgattgaag ttaacgtggc cgcgaaggat gccgctacag
    12421 ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc cttccttgaa ctcaatcaga
    12481 tcgcgaaaaa agatatcagc tcagcgaatt tacgcaccca gccagattat gactatcagg
    12541 atggtaaaag tatcctgaaa ggctaccgag ctgtgagaac ggtggaagtc acgctccgtc
    12601 agttagacaa actgaattcc ttgctggatg gcgcgctgaa ggcgggtctt aacgaaattc
    12661 gttctgtgtc gctgggcgtg gcgcagccgg atgcctataa agacaaagcg cgtaaggcag
    12721 cgattgataa cgcgattcat caggcgcagg aactggcgaa cggctttcat cgtaaactgg
    12781 ggccggtata tagcgtgcgc taccatgttt ccaactatca gcccagccca atggtgcgga
    12841 tgatgaaagc cgatgccgcg ccggtgtctg cccaggaaac ttacgagcag gccgctattc
    12901 agtttgatga tcaggtcgat gtggtcttcc agttagaacc tgtggatcaa caacccgcta
    12961 aaacacctgc agcacaataa tacttgtagc ccgacgtatt cgtcgggcta atgcctttac
    13021 ccgatattgc ctcctttttc tgtaattttc tgtaaaacca tgacgtaatc acctatcttt
    13081 gctcgcggtt tcatcaagga taggctgact gaaacggtta agcggacaag gatgcgggga
    13141 ggggggatgg acatttttat ttcgaaaaag atgcgcaatt tcattttatt agcgcaaacc
    13201 aataatattg cccgggcggc agaaaaaatc catatgacgg cttcgccatt tggtaaaagc
    13261 attgccgcgc tggaagaaca aattggctat acgctattta cccgcaaaga taataatatc
    13321 agcctcaata aagccggtca ggaactgtat caaaaactgt tcccggttta tcaacgactt
    13381 tctgcgattg ataatgaaat ccataactca gggcgtcgtt cacgagaaat tgtgattggc
    13441 atagataata cttatccgac aattattttc gatcagttga ttagtctcgg agataagtat
    13501 gaaggcgtga ccgcccagcc agttgagttc agcgaaaacg gcgttatcga taatcttttt
    13561 gaccgtcagt tagattttat tatctcccca cagcatgtat ctgcacgagt tcaggaactg
    13621 gaaaacctga ctatcagtga attaccaccg ctgcgattgg gctttctcgt ttctcgccgt
    13681 tatgaagagc gtcaagagca agagctgctt caggagctgc cctggctaca aatgcgcttt
    13741 caaaaccggg ctaacttcga agcaatgatt gatgcgaata tgcgaccttg tgggatcaat
    13801 cccaccatta tttatcgtcc ctacagtttt atggccaaaa tcagcgccgt ggagcgcgga
    13861 cattttctga cggtgatccc gcattttgcc tggcgactgg tgaatccggc aacgctgaaa
    13921 tattttgatg cgccgcacag gccgatgtat atgcaggaat atctttattc aatcagaaat
    13981 catcgttata ccgccacgat cttcagcata ttgctgaaga tcgtgacggg acaaaccatt
    14041 aacccagcat cgagccggtt gcaattaaat tacggtgtaa gtcgaagacg tggctaagat
    14101 cgtggtgaat atgtccgccg ggcgcatttt ccagatagcg atgcagataa tcccgataca
    14161 taggatgtgc acaattatca atgatagtgc gggcgcgttg aagcggggaa agaccgcgca
    14221 gatcggcgat cccttgttcg gtgatgatca ctttgacgct gtgttcgctg tgatcaacat
    14281 ggctgcacat tggcacgacg gttgagatct tgccttcttt agcaatcgac ggagccataa
    14341 agatcgacag atacgcgttg cgttcaaaat caccgctgcc gccgatgccg ttcatcagat
    14401 cgaccccggc tacgtgcgtt gaatttgcat gcccgtaaat atcaaactcc aggccgacgt
    14461 tcagagcgat gacgcccaga cgacggatga tttccgggtt attggaaatc tcctgcggac
    14521 gcaacacaat gcggctggca aagtaatcca tattgtcgta aatcttgcgc agggaatcgg
    14581 ccgagattgt caggctggag gcgctggccc cgctgatttt gccggtttcc agtaaatgca
    14641 ccaccgattc ctgtagcact tccgaataca tcatgaacgg aggaatttcc gggttttccc
    14701 ccagacgtgc cattaccgca ttattgatat tgcccacgcc actttgcagc ggcagaaatt
    14761 ccggcggaat acgcccatgc gccatttcct gcaataagaa cgtgaccacg ttatcggcaa
    14821 tctgctggca catgggattt tgcttatcca gcatattacc ggcgtcgggc aagttggttt
    14881 ccacgacggc gacaatcttt ttcggatcga tttgcacata gcgggtaccg acgcgatcca
    14941 ttgcatggaa gatcgacacg ctattgcgcc gcggtggcgc gccaggaatc acaatatctg
    15001 ccagttctgc aacgcgcgga tcgtgatagt gattaagttc aatgatcact ttcttcgctc
    15061 gcagcagcca ggtcggcgca ttaccgatcc cgctggttaa ccagactcga ccatccggtg
    15121 ccagtgccga tgcttcaatg acggcaacat caatgtcgcc gaagaaaccg taattgacca
    15181 tttgcgccac ttcgctcaaa tgcaggtcaa cgaaactcac cgcgccctga ttgatctttt
    15241 tacgtaaact ggacgatgtt tgatatggcg cacgccagga aacagcatcg gcgtcagaaa
    15301 gtacatcgtc agcggcggcg ctgattgacg cccccgtcag caggcgaatt tgatacggct
    15361 ttttggcctc atgctgttcg ttagctctgc gggcaatcgc ggcgggtagg gctttcggcg
    15421 aacccgctgg ggtaaagccg ctaaatgcca ccatgtcgtt atgctggata atttctgccg
    15481 cttcatcggc ggtcatcctt gtccactgag tttccatttt tactccttcg ttcgcaggga
    15541 ttaatgacca acgaaattag gtttacgttt ttcgaggaaa gcgttcatcc cttcctggta
    15601 atcttcgctg tcataaaccg cgcggcgcat cccctgaata cgttcaaatt catcggagtt
    15661 catggtgtgt gcttcgccca gtacacgcag ctcttctttg ataacggcaa tggctaacgg
    15721 cgctttctca gagatgtggt gcgccatttg taaggtgaaa tcttccagtt cttccacttc
    15781 cacaacatgg ttgaggatgc cgacagccag cgcgcgctgg gcggtgattg gcgaagcggt
    15841 aaaaatcagc tctttgacaa tgtggaagcc cgcgtcgcgg gtcaggttgt gaatgccgac
    15901 caggttatac gggacgccga ggtttacagg cgtcattgag aaggttgagg tactggcggc
    15961 gatgatcaga tcggaactca tgatcatttc aaatgcgcca ccccaaacac taccttccac
    16021 catcgaaatg atcggtttcg ggaatttttg gatcatgcgg gtgatttgac gcaatggatc
    16081 atcataggag agcggatcgc gaccgccaga cggcagttcg tgaatatcgt gacctgcgga
    16141 gaagactttg gatccacttg gcgcccgcaa aatgatacag cgaatttccg gccggttgag
    16201 atcgcttaac gcctgcataa gatcatcaat aaagacttta cttaaggcat taagttttcg
    16261 tccatagtta aactcaatga ccgccacttt gttgatagtg gcaacgttca catactgata
    16321 agacataaaa attcctttaa tcaaaatatt gcgtctggat aaattcactg agctgccgca
    16381 ggccggtgcg cggtgagagc gtattgtttt tgaccgctaa aagcgtctgg cggtaatagc
    16441 gatcgaaatc ttcattcgcg aacaggtgat tcagtacttc ttcttcggtc tgcttacgca
    16501 gccattccac cgattgttgt tgccgcactt gttgtaaacg accactggca gttagcgcgg
    16561 ttttgaagtc gatgatggcg tgccagatct catcgattcc acgtttttcc agtgcgctac
    16621 aagtcagacc ccgtggctgc cattcgtcgt atttacgtcg cagaatatgc agggcactct
    16681 cgtacatatg ccgggcaatg gcgacattgg tatggttatc gccatcgtct ttgttgataa
    16741 cgagcagatc agccacttcc atcagccctt ttttaatgcc ctgcagatca tcgccgccac
    16801 cggcaatttg caacgagata aaacagtcca ccatgcgggc gacttccgtt tccgactgcg
    16861 cgacgccaac cgtttcgaca atcactacgt cataacccgc cgcttcgcat aacagcatta
    16921 attcccgtgc tcgctgactg gcaccgccca gatgaccgga ggagggtacc gggcgaataa
    16981 acgccgcctc ggcacgcgcc aggtcattca tgcgggtttt atccccgaga atgctaccgc
    17041 cagtgaccgg gctgctggga tcgaccgcaa taacagcaac ctttaatccc tcacgaatca
    17101 acaacatgcc aaaggcttca agaaaggtac ttttccccgc gccgggtgtg ccggtaacgc
    17161 ccagtcgcag ggcgttacca cagaacggca taatagcgtc aagcagctgt gtacttagcg
    17221 cctgatgacg tgggtgacgg ctttccacca gcgtcatggc ttgggcgagc gtggcacgct
    17281 caccctgacg taagcggcga atactttctg ccagcgtggc ttcattaatc atgatgctgg
    17341 cttatcagat tcagtacgtc gcgcacactg tcgagcatag gtgtacctgg accataaatc
    17401 gccgccacgc cgcgctcttg caggaaggcg taatcctgcg gcggaatgac gccacccgcg
    17461 accacgcaga tatcttcgcg tccccatttt ttcagcgctt cgaccagttc cgggatcagc
    17521 gttttatgac cggcagccag tgaggatgcg cccactacgt gaacgtcgtt ttctacggcc
    17581 aggcgggcga tctcttcagg tgtagagaac atcgggctta aatctacgtc gaaaccgaga
    17641 tcggaatagg cgctggcgat cactttcgcg ccgcgatcgt gtccatcctg gcccatctta
    17701 gcgatcagaa tgcgcgggcg acgaccattg tcggcaagga actgctccgt ttgcgcaaca
    17761 atggcatcga actcggaggc cgatttctca gactgatgat agctttgcgc aatcacgccg
    17821 gtaacacact ggcttggcac cagataacgg tcgaaagcga cttccagcgc atcggaaatt
    17881 tcacccaggg tggcgcgaac gcgagcggca ttaacagcgg cagccagcag gttttcgtta
    17941 tgctgtgcgg cgtgagtcag ggcgttcaac gcggcggtta cggcggcatc atcacgggtg
    18001 gcgcgaatgc gttccagcga agcaatttgc tcgttacgca ccatcacgtt gtcgatctca
    18061 agtacatcgg tttcgtcttc gtgatccagt ttgtacttgt tgacaccaac gatgacacgc
    18121 ttgccctggt cgatcagcga ctgttcgcgc gctgaggcct cttcgatcat tcgttttggc
    18181 agacctgctt cgatcgcttt cgccatgcca ccggcttcgt cgatctgttg gataatagct
    18241 ctggcttgtt tgacgatttg atcggtcagc gactcaatgt aataggatcc ggccagtgga
    18301 tcgacggtgt ggcagagttc tgattcttcc tggatgatga tctgggtgtt gcgggcaatg
    18361 cgtgctgaga aatcggtagg caaaccaagc gcttcgtcaa aggcgttggt atgcagtgac
    18421 tgagtaccgc ccagcgtcgc agccagtgct tcaatggtgg tgcggataac gttgttatac
    18481 ggatcctgtt cagtcaggct ccagcctgag gtctggcagt gggtacgcag cgccagtgat
    18541 ttcggatcct gtgcgccaaa tccactgacc gcttcgctcc ataaataacg tgccgcacgc
    18601 aacatggcga cgttcataaa cagatccatg ccgataccga agaataacga caggcgagga
    18661 gcgaagtcat caattttcag tccggcagag attgctgctt tgatgtactc aatcccatcg
    18721 gcgagcgtaa atgctacctg ctgcacgcag ttggcacccg cttcccccat gtggtaaccg
    18781 ctgatactga tggtattaaa tcgcggcatg ttgccggaac accaggcgat gatgtcggcg
    18841 ataatgcgca ttgacggttt tggtgggtaa atataggtgt tgcggcagag gtactctttg
    18901 agaatatcgt tttgaatggt gccggtcagt ttatcaggtg taacaccttg ctcttctgcg
    18961 gcgacgatat aaaacgccag tactggtagc actgcgccat tcatggtcat cgaaaccgac
    19021 attttatcca gcgggatctg gtcgaacagg actttcatat cttccacggt gtcgatagcg
    19081 acgcccgctt tgccgacgtc acccgccacg cgtgggttat cggagtcgta gccacggtgg
    19141 gtggcaaggt caaacgcaac ggaaagacct ttttgcccgg cggccaggtt acggcgataa
    19201 aaagcgttgg actcttttgc tgttgaaaaa ccagcatact gacggatggt ccacggttgg
    19261 gcggtataca tagtggcacg cgggccacga acgtagggcg gcaaaccagg aagggtacct
    19321 gtcacctcca gattatcgag atcggcttcg gtatacagcg gcttgatggc gatcccttcc
    19381 gcggtttgct gaaccagcga gtcgacagtt ttctcccgac ggctcaattc cttgttggca
    19441 agctgttgcc actcctgctc gttagacatt atttgctccg taaaacgcaa tcaaaatgag
    19501 ggtatttgcg cttacggtga gctttttacg tgggtttgtc gccagcaaaa aaaatgcatt
    19561 cagacgtcgt tttttgccgc ctgagatttg tctattttgt gatgtgattc aaggaataca
    19621 aaaagaagtg aggacaacgc ctgatatgtg ctatcaggca ttatttgatg gattaatcct
    19681 gacgaaggac tttgtgacca taatcgagta acgcatcagt gactttacgc atcatgcggc
    19741 tttcaggagc aaagcggtgc cagtagagca tccgtcgttg aaatagccca ggcgttaagt
    19801 caatcagttc accactggcc agctctttct cgatttgcag gtgcgggatc atacagcagg
    19861 tggtgccctg gcgagcaagt tgtacgaacg cttctgaaga attaacgata tggcagggta
    19921 cgctgccagg aggaagatcg aagttttgct gcaaaaaggc ctggtgcata tcgtcaagat
    19981 ggtcaaacgc gaccactggc gctttcaata atgccgaacg cgttacgccg ttaggaaaat
    20041 atttttcggc aaacggtttt gagctgacga acagatagtc gagcgcaccg agtttatcga
    20101 caagacaact cggcagcgcc tgatgttgaa tactcaccgc gccgaccact tcgccgcggc
    20161 gcagacgttc ctgagtacgg gtttcatctt ctacctgcaa gttgaggcgg ataggcgaat
    20221 cagccaacac aggagccagt gccggaagca accacgtcgc cagactgtcg gcgttgaccg
    20281 ccagtgaaag cagcagcgga gtcgaaccgg tttgttcatc gcccagccac tcttcttcca
    20341 gcaactccac ctggcgcagc agtgccagca gtttttgccc ttgttccgtc gggcgcggcg
    20401 gtacggtacg caccaacagc ggctgcccga acatattttc cagttgctta atgcgctgtg
    20461 agacggctga ttgtgtaatg cacagctttt gtgccgcgcg ctcaaatcct cgttcacgta
    20521 tcaccgcatc cagtgcctgt aatgttctgt agtccgggcg tttcattgct gttgtctgct
    20581 cctggtaaat gctttttcct gcactatgac acaattttgt gtcaggttgc atataccgaa
    20641 gccgtcggcg ggtaacattt gtatcaatgg ttaaacgcgc agcttaaaag atcagggatt
    20701 tgcgctattg catttcgctt agcgacttcg cgccctggtt atgttgaatg gcgtggcgtt
    20761 attgcctctg ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg
    20821 gcgaaagggg atgcccgcca ttgcgcgata ttttatggat gagttaacca cccggcttgc
    20881 caacggggtc tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg
    20941 aaacgatcat gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg
    21001 ttcagcccgg taccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg
    21061 cgctcggcac gatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg
    21121 aaaaactgaa aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca
    21181 tctacgttga tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg
    21241 cggctctgac ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgccg
    21301 acgcctccaa gcaggttgat attctgggta aattcccgct gccagtagaa gttatcccga
    21361 tggcacgtag tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc
    21421 agggcgtggt gaccgataat ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg
    21481 acccgatagc gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt
    21541 ttgctaaccg tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg
    21601 tgaaatgatc tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg
    21661 acatgtgtca cgcttttacc aggcaattgt cgattgctct aaataaatcc tctaaaccag
    21721 catattcatc caagaattac ctttgcgtga tatttcctca acatcgcgac gcaaacgttc
    21781 atattgccgc aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca
    21841 tttcaaaaga caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt
    21901 ttctgctggt agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca
    21961 ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg
    22021 atgcccactt catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg
    22081 cagaaaaact ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg
    22141 cggcggcaaa gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg
    22201 cggagctggt gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta
    22261 aagcgcaccg tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa
    22321 agctgggtat catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc
    22381 tgggaatgta tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc
    22441 aggtacagca tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag
    22501 agaatccgtc caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aagcccggct
    22561 cgctgctgat taatgcctcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc
    22621 tggcgagcaa acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca
    22681 atagcgatcc atttacctct ccactgtgtg aattcgacaa cgtccttctg acgccacaca
    22741 ttggcggttc gactcaggaa gcgcaggaga atatcggctt ggaagttgcg ggtaaattga
    22801 tcaagtattc tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc
    22861 cactgcatgg tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg
    22921 cgctgaacaa aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt
    22981 ccgcccagat gggttatgta gttattgata ttgaagctga cgaagacgtt gccgaaaaag
    23041 cgctgcaggc aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc
    23101 ccttctctga aaattaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac
    23161 accgttgccg cgttgcgcat tctactttgc gagtctgctc cagaaaataa atcaaagtca
    23221 gatcaatgcg ttgtgtttat ttaatgggga tttacgatgg cgggacagca tggggctgta
    23281 acggtaaagc cctcaccgaa gcgagggctt aaaggagaag ggttatgatg cgacttgtca
    23341 tcatactgat tgtactgtta ctgataagtt tcggagctta ttaacagtca gtctcaaggg
    23401 aggagtcatc ctcccttacc cttactcact aaattaggtc aaagaatcaa cgatgtcaat
    23461 cagggcgatg cggggtgcat cgccctgaac cgaacttttt gatagccact tcatcgccca
    23521 cgtttactgc cgtaatagat cctttttccc aaaaattatc tctgttttgc atggggtttt
    23581 tcgagcacgc tcgaagaaaa gatttaaagg tagatcaatg cgttgtggtt atttaaagag
    23641 agcttacgat agctgggcag catggggctg taacggtaaa gccctcaccg aagcgagggc
    23701 ttgaaggaga agggttatga tgcgacttgc catcatactg attgtactgt tactcataag
    23761 tttcccagct tattaacagt cagtcacagg ggaggagcaa tcctccctta cccttactca
    23821 ctaaattagg ttaaagaatc aacgatgtca atcagggcga tgcaggtgta tcgcccttac
    23881 cactcccaga ctttcgacgg tgtaaccacc gcaggaagag ggatatccca ctcttcaacg
    23941 gggagttttt ccaccaactg acaatcatgc gcataaccca ccggttgcgt tttatagtgc
    24001 tgccagtttt gtaaggtccg atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac
    24061 tcatcaaagg cgaccagcgg tgtgatcagc acgtctaatc gggaaagggg tagcacgtca
    24121 cgcacatcca gttttggctc atggatcttc aacctgttca tcaccagttc gctttgcgga
    24181 tggtaattca ggaacagcaa attaccggca ctaaagggat gcaaaactgg aagatatacg
    24241 cgcttactgg cgcgccagag ttgttctatt agcggctggg tgtcgagttc accatcaaaa
    24301 gagaggaata cagcgaccgt atgcgccatc accaccgggg gataagtcat catccgggta
    24361 gcggcttgtt gacccatttc cagctgttgt tccggcgtta acgcacgacg acgttgccga
    24421 atcatttttc ggatttcttg tcgggataat gtcagtggga gctctggtag ttgcgtcatg
    24481 gctggtgcca gataagaagg gaatctccga gatgccgccg caggctgtaa cccttgaacc
    24541 cttggttcaa ggtgaatgtg tcgtcgcagt tttaaggctt ctcggacgaa ccgagcatgc
    24601 tcaccaaccg cggagcgcca cattcttgtg gtatgaaata tcggctcagg ggactggccc
    24661 gcttgcgaac atctcagaga aattttgtct tcacggttac tctaccacag taaaccgaaa
    24721 agtgtcattc aaagttttgg ttagtttttt cggtgatgcg accttgttca agtaacgctt
    24781 gttctatggt ctgctgcagc atccgaatac gctgttccat acttgccgcg tagtcacgag
    24841 tctttgcttt ttcttgcgct aactcatagc tgatattcaa tgcggcaatg aagaccaact
    24901 gttcagtatt tgtgactcta gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt
    24961 ccgctgcctg attcaacgca tccctttggt caggcgggca gttcacacgc agtgaacggc
    25021 caaaaatttg gatatcgacg ggttgtgcag acatgccacc ttcctgctga ttgactgcgc
    25081 tgccttcgtc tttagaccca tggtctgcga aggggcgaca ctatagctac cctgatgaga
    25141 agagacaagc ccttttctgg tccaccaggg cccaaagtgg tagcatatca tgaatattcc
    25201 tccctttgac gacgaatgct tatgtctata cagaacgaaa tgcctggtta caacgaaatg
    25261 aaccagtatc tgaaccaaca agggacgggt ctgacccctg ccgagatgca tggtttaatc
    25321 agtgggatga tatgtggcgg taacgatgac agctcatggc tgccgctact tcacgacctg
    25381 acgaacgaag gcatggcttt cggtcatgag ctggcacagg cactgcgtaa aatgcactct
    25441 gccaccagcg atgccctgca ggatgacggc ttcctttttc agctttatct gcctgatggc
    25501 gatgatgtca gcgttttcga tcgggctgat gcactggcag gttgggtcaa tcacttcctg
    25561 cttggtcttg gcgttacgca accgaagctg gacaaagtga ccggcgaaac cggtgaagcc
    25621 atcgacgatc tgcgtaacat cgcgcagctg ggctacgacg aagacgaaga tcaggaagag
    25681 cttgaaatgt cgcttgaaga gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac
    25741 gacaccttta ctcatccgca accgaccgcg ccagaagtac aaaaaccgac tctacactaa
    25801 aaacaaaaaa cgtaaggaga gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg
    25861 ccgtcaggcc ctggtggagc aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc
    25921 agaagtaaca cgtagcgccg acagcgaata cccctatcgt cagaacagtg acttctggta
    25981 cttcaccggc tttaacgaac cggaagcggt gctggtgctg attaaaagcg atgacactca
    26041 taaccacagc gttctgttta accgcgttcg cgacctgacg gcggagatct ggtttggccg
    26101 tcgcttaggc caggatgccg cgccagagaa actgggcgtt gaccgcgcac tggcattcag
    26161 cgaaatcaat cagcaacttt atcaactact taacggcctg gatgtggttt accatgccca
    26221 gggcgaatat gcatatgctg atgaaatcgt gaacagtgcg ctggaaaaac tgcgtaaagg
    26281 ttcgcggcaa aatctcaccg caccggcaac gatgatcgac tggcgtcctg ttgttcatga
    26341 aatgcgcctg ttcaaatcgc cagaagagat tgccgtactc cgccgcgcgg gagaaatcac
    26401 cgccatggca catacacggg cgatggaaaa atgccgtccg ggaatgttcg agtaccatct
    26461 ggaaggcgaa attcaccacg aatttaaccg ccacggtgcg cgctatcctt cctataacac
    26521 cattgtaggc agcggtgaaa acggctgcat tctgcactac accgaaaacg agagtgaact
    26581 gcgcgacggt gacctggtgt tgattgacgc gggttgtgaa tacaaaggtt acgcgggcga
    26641 tattacccgc accttcccgg tcaacggcaa attcacccag gcccagcgtg aaatctacga
    26701 cattgtgctg gagtctctcg aaaccagcct gcgcctgtat cgtccgggaa cttccattct
    26761 ggaagtcact ggtgaagtgg tgcgcatcat ggttagcggc ctggtaaaac tcggcatcct
    26821 gaaaggtaat gttgatgaac tgatcgctca gaacgcccat cgtcctttct ttatgcatgg
    26881 ccttagccac tggttaggac tggatgtcca tgacgttggc gtttatggtc aggatcgctc
    26941 gcgcattctg gaaccgggga tggtactgac cgtagagcct ggtctgtata tcgcgccgga
    27001 tgcggatgtg ccagaacaat atcgcggtat cggcattcgc attgaagacg acattgtgat
    27061 taccgaaacc ggcaacgaaa acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga
    27121 agcgttgatg gctgctgcga gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg
    27181 ggcgcgacgc tggcgctggc tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg
    27241 attgaagcga ctgcgccaga gtcacatgct catccgggct ttgatggacg agcgatagcg
    27301 ctggcggcgg gtacctgtca gcaactggcg cgcatcggcg tctggcaatc tctggctgat
    27361 tgcgcaaccg ccatcactac cgtgcatgtc agcgatcgtg gtcatgctgg attcgtcacc
    27421 ctcgccgcag aagattacca actggcggcg ctggggcagg ttgtcgaatt gcataatgtc
    27481 gggcaacggc tgtttgcatt gctgcgaaaa gcacctggcg taatgctgca ttgccccgat
    27541 cgcgtagcta acgttgcccg tactcagagt catgttgaag tgacgctgga gagcggcgag
    27601 atgctgacgg gccgcgtgct gatagccgct gatggcaccc attcagcgtt agccaccgcg
    27661 tgcggcgttg attggcagca ggagccttac gaacaactgg cagtgattgc caacgttgct
    27721 acttctgttg cgcacgaagg ccgcgctttt gaacgcttca cgcaacatgg cccgctggcg
    27781 atgttgccga tgtctgacgg acgctgttcg ctggtctggt gtcatccact ggaacggcgc
    27841 gaagaggtgc tgtcgtggag cgacgagaag ttttgccgtg aactccagtc tgcctttggc
    27901 tggcgactgg ggcaaattac ccacgccggt aaacgcagtg cttatccgct ggcattaacc
    27961 cgcgccgcca gaccgattac ccatcgcact gtgctggtgg gcaatgcggc gcaaactctg
    28021 cacccgattg ccgggcaagg gtttaacctc ggtatgcgag atgtcatgag ccttgcggaa
    28081 accctgactc aggcgcatga gcgcggagaa gacatcggtg attacggcat attgtgccgc
    28141 tatcagcagc gtcgacagag cgatcgcgaa gcaaccatcg gcgtcacgga cagcctggta
    28201 catctttttg ccaaccgttg ggcaccgctg gttgtcgggc gcaacatcgg actgatgacg
    28261 atggaattat tcaccccggc acgcgatgtg ctggcgcagc gcaccctcgg ttgggtggcg
    28321 cgttgaaggc ggttttaagg aataaacaat gcaaagtgtt gatgtagcca ttgttggcgg
    28381 cggcatggtg gggctggcgg ttgcctgcgg cttacagggg agcggcttac gcgttgccgt
    28441 actggagcag cgcgtaccgg aacctctggc ggtagatgca ctaccacaac tgcgcgtttc
    28501 ggctatcaat gccgccagcg aaaaattact cacccgtctt ggcgtctggc aggacattct
    28561 cgctcgtagg gccagttgtt atcacggcat ggaagtgtgg gacaaagaca gttttggtca
    28621 catctcgttt gacgatcaaa gcatgggcta tagccatctt gggcacatcg ttgaaaactc
    28681 agtgattcac tacgcgctgt ggaacaaagc gcagcagtcg tcagatatta ctctgttggc
    28741 ccccacagaa ttacagcagg tcgcctgggg agaaaatgaa accttcctga cgctgaaaga
    28801 tggcagcatg ttaacggcgc gtctggtgat tggcgcggac ggtgctaatt cctggttgcg
    28861 caacaaagca gatattccgc tgactttctg ggattatcag catcacgcgc tggtagcgac
    28921 cattcgcacg gaagaaccgc atgatgcggt ggcgcggcag gttttccatg gcgaaggcat
    28981 tctggccttt ttaccgctta gcgatccgca tctttgctcg attgtctggt cactgtcgcc
    29041 agaggaagcg cagcggatgc agcaggcaag tgaagacgaa tttaatcgcg cgttaaatat
    29101 cgcttttgat aatcgccttg gcttatgcaa ggttgagagc gcgcgtcagg tgttcccgct
    29161 gacggggcgt tatgcgcgcc agtttgccgc acaccgtctg gcgctggtgg gcgacgccgc
    29221 gcataccatt cacccactgg cggggcaggg cgttaatctc ggatttatgg acgccgcaga
    29281 gctgattgct gaactgaaac ggttgcatcg tcagggtaaa gacatcgggc agtacattta
    29341 cctgcgccgc tatgaacgta gccgcaagca cagcgcggcg ctgatgctgg ctggtatgca
    29401 gggttttcgc gatctctttt ccggcgctaa tccggcgaaa aaactgctgc gtgatattgg
    29461 tttgaaactg gccgacacgc ttcctggcgt taagccgcaa cttatccgcc aggcaatggg
    29521 attaaacgat ttgcctgaat ggctgcgtta aaaatttctc ctctgttgtt tatttgatac
    29581 ccatcacact ttcatctccc ggttttttcg ccgggagatt ttcctcattt gaaataaact
    29641 aatttcacct ccgttttcgc attatatttt ctaataccat gattttttga tttagtgttt
    29701 tttgacattt ttttagctct taatattgtc ttattcaaat tgactttctc atcacatcat
    29761 ctttgtatag aaattggtgt attttttggt tttttattct gtcgcgattt ttgcattttt
    29821 taaccataag ctaatgtgat gatcaatttt accttatggt taacagtctg tttcggtggt
    29881 aagttcaggc aaaagagaac gattgcgttg gggaccggga gtggctccga tgctgggttt
    29941 tgtggtgata atttcaccat gaaaaagttg tcagccccgc ttattcaatg aggacaagat
    30001 ggcacaacag actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt
    30061 ccacggctgg atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg
    30121 taccgatgcc ggaatgtttg atgtgtcgca tatgaccatc gtcgatctcc gcggcagccg
    30181 cacctgggag tttctgcgtt atctgctggc gaacgatgtg gcgaagctca ccaaaagcgg
    30241 caaagccctt tactcgggga tgttgaatgc ctctggcggt gtgatagatg atctcatcgt
    30301 ctactacttt actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga
    30361 cctctcctgg attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga
    30421 cctttccatg attgccgtgc aagggccgaa tgctcaggca aaagctgcca cactgtttaa
    30481 tgatgctcag cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc aggtgggcga
    30541 tctgtttatt gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa
    30601 tgaaaaagcg gccgatttct ggcgtgcgct ggtggaagcg ggcgttaagc cctgcggtct
    30661 gggggcgcgt gacacgctgc gtctggaagc gggcatgaat ctttatggtc aggagatgga
    30721 cgaaactatt tctcctttag ccgccaacat gggctggact atcgcctggg aaccgacaga
    30781 tcgtgacttt atcggtcgtg aagccctgga agtgcagcgt gagcatggta cagaaaaact
    30841 ggttggtctg gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgctttac
    30901 cgatgcgcag ggcaaccagc atgaaggcat tatcaccagc ggtactttct ccccgacgct
    30961 gggttacagc attgcgctgg cgcgcgtgcc ggaaggtatt ggcgaaacgg cgattgtgca
    31021 aattcgcaac cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc gtaacggcaa
    31081 agccgtcgcg tgatttactt ttttggagat tgattgatga gcaacgtacc agcagaactg
    31141 aaatacagca aagaacacga atggctgcgt aaagaagccg acggcactta caccgttggt
    31201 attaccgaac atgctcagga gctgttaggc gatatggtgt ttgttgacct gccggaagtg
    31261 ggcgcaacgg ttagcgcggg cgatgactgc gcggttgccg aatcggtaaa agcggcgtca
    31321 gacatttatg cgccagtaag cggtgaaatc gtggcggtaa acgacgcact gagcgattcc
    31381 ccggaactgg tgaacagcga accgtatgca ggcggctgga tctttaaaat caaagccagc
    31441 gatgaaagcg aactggaatc actgctggat gcgaccgcat acgaagcatt gttagaagat
    31501 gagtaacggc tttattcctc ttctgcggga gaggatcagg gtgaggaaaa tttatgcctc
    31561 accctcactc tcttcgtaag gagagaggtt cacaattcac tgcacgtttc aggaaccatc
    31621 gctcatgaca cagacgttaa gccagcttga aaacagcggc gcttttattg aacgccatat
    31681 cggaccggac gccgcgcaac agcaagaaat gctgaatgcc gttggtgcac aatcgttaaa
    31741 cgcgctgacc ggccagattg tgccgaaaga tattcagctt gcgacaccac cgcaggttgg
    31801 cgcaccggcg accgaatacg ccgcactggc agaactcaag gctattgcca gtcgcaataa
    31861 acgcttcacg tcttacatcg gcatgggtta caccgccgtg cagctaccgc cggttatcct
    31921 gcgtaacatg ctggaaaatc cgggctggta taccgcgtac actccgtatc agcctgaagt
    31981 ctcccagggc cgccttgaag cactgctcaa cttccagcag gtaacgctgg atttgactgg
    32041 actggatatg gcctctgctt ctcttctgga cgaggccacc gctgccgccg aagcaatggc
    32101 gatggcgaaa cgcgtcagca aactgaaaaa tgccaaccgc ttcttcgtgg cttccaatgt
    32161 gcatccgcaa acgctggatg tggtccgtac tcgtgctgaa acctttggtt ttgaagtgat
    32221 tgtcgatgac gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc tgttacagca
    32281 ggtaggcact accggtgaaa ttcacgacta cactgcgctt attagcgaac tgaaatcacg
    32341 caaaattgtg gtcagcgttg ccgccgatat tatggcgctg gtgctgttaa ctgcgccggg
    32401 taaacagggc gcggatattg tttttggttc ggcgcaacgc ttcggcgtgc cgatgggcta
    32461 cggtggccca cacgcggcat tctttgcggc gaaagatgaa tacaaacgct caatgccggg
    32521 ccgtattatc ggtgtatcga aagatgcagc tggcaatacc gcgctgcgca tggcgatgca
    32581 gactcgcgag caacatattc gtcgtgagaa agcgaactcc aacatttgta cttcccaggt
    32641 actgctggca aacatcgcca gcctgtatgc cgtttatcac ggcccggttg gcctgaaacg
    32701 tatcgctaac cgcattcacc gtctgaccga tattctggcg gcgggcctgc aacaaaaagg
    32761 tctgaaactg cgccatgcgc actatttcga caccttgtgt gtggaagtgg ccgacaaagc
    32821 gggcgtactg acgcgtgccg aagcggctga aatcaacctg cgtagcgata tcctgaacgc
    32881 cgtggggatc acccttgatg aaaccaccac gcgtgaaaac gtaatgcagc ttttcagtgt
    32941 gctgctgggc gataaccacg ggctggagat cgacacgctg gacaaagacg tggctcacga
    33001 cagccgctct atccagcctg cgatgctgcg cgacgacgaa atcctcaccc atccggtgtt
    33061 taatcgctat cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg agcgtaaaga
    33121 tctggcgctg aatcaggcga tgatcccgct gggttcctgc accatgaaac tgaacgccgc
    33181 cgccgagatg atcccaatca cttggacgga atttgccgaa ctgcacccgt tttgcccgcc
    33241 ggaacaggct gaaggttatc agcagatgat tgcgcagctg gctgactggc tggtgaaact
    33301 gaccggttac gacgccgttt gtatgcagcc gaactccggc gcacagggcg aatacgcggg
    33361 cctgctggcg attcgtcatt atcatgaaag ccgcaacgaa gggcatcgcg atatctgcct
    33421 gatcccggct tctgcgcacg gaactaaccc cgcttctgca catatggcag gaatgcaggt
    33481 ggtggttgtg gcgtgtgata aaaacggcaa catcgatctg actgatctgc gcgcgaaagc
    33541 ggaacaggcg ggcgataacc tctcctgtat catggtgact tatccttcta cccacggcgt
    33601 gtatgaagaa acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg gtcaggttta
    33661 ccttgatggc gcgaacatga acgcccaggt tggcatcacc tcgccgggct ttattggcgc
    33721 ggacgtttcg cacctcaacc tgcataaaac tttctgcatt ccgcacggcg gtggtggtcc
    33781 gggtatggga ccgatcggtg tgaaagcgca tctggcaccg tttgtaccgg gtcatagcgt
    33841 ggtgcaaatc gaaggcatgt taacccgtca gggcgcagtt tctgcggcac cgttcggtag
    33901 cgcctctatc ctgccaatca gctggatgta catccgcatg atgggcgcag aagggctgaa
    33961 aaaagcaagc caggtggcaa tcctcaacgc caactatatt gccagccgcc tgcaggatgc
    34021 cttcccggtg ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta ttctcgatat
    34081 tcgcccgctg aaagaagaaa ccggcatcag cgagctggat attgccaagc gcctgatcga
    34141 ctacggtttc cacgcgccga cgatgtcgtt cccggtggcg ggtacgctga tggttgaacc
    34201 gactgaatct gaaagcaaag tggaactgga tcgctttatc gacgcgatgc tggctatccg
    34261 cgcagaaatt gaccaggtga aagccggtgt ctggccgctg gaagataacc cgctggtgaa
    34321 cgcgccgcac attcagagcg aactggtcgc cgagtgggcg catccgtaca gccgtgaagt
    34381 tgcggtattc ccggcaggcg tggcagacaa atactggccg acggtgaaac gtctggatga
    34441 tgtttacggc gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat accagtaatt
    34501 cactgattcg agtattttct aaaggcgctt cggcgccttt ttagtcagat gacaaagtac
    34561 aaaagtgctc agacagtccc ctcgcccctt tggggagagg gttagggtga ggggaacagg
    34621 ccggcactgg cgcgaatatt taccctcacc ccgaccctct ccctgaaagg gcgaggggga
    34681 aaagcgtgcc aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg
    34741 aattaatttt tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc
    34801 atcgggcggg caactgcatt actgttggcg caagaagggt atacggtggc ggttaattat
    34861 cagcaaaacc tccacgcggc gcaggaagtg atgaacttaa taatgcaagc cggtggcaaa
    34921 gcattcgtgc tccaggcgga tatcagcgac gaaaaccagg tcgttgcgat gtttacagca
    34981 atcgatcagc acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc
    35041 cagtgcaccg ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg
    35101 acgggatatt ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc
    35161 agtggcggcg ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa
    35221 tatgttgatt acgcggcatc gaaaggggcg attgattcgt taaccaccgg actatcgctg
    35281 gaagtcgccg cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa
    35341 atgcacgcca gcggcggcga gcctggacgc gtcgatcgcg ttaagtcgaa catccccatg
    35401 cagcgtggtg gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa
    35461 gcctcttacg tcacgggaag ttttatcgat ttggcgggcg ggaaataaaa cagggaagtt
    35521 gtctgaccgg atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg
    35581 ttgctggcaa tcacctcttt gtaccagtta aagctcttct tacgtgaacg cgacatatcg
    35641 ccagtaccgt cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg
    35701 gtagtgaatg acacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacacca
    35761 tcgtaagtca ctgctttttt catctcttca atatgggcgc gcaggtagtc aatgcggtag
    35821 tcgtcgttga tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca
    35881 ataaacagcg gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact
    35941 ggatcaatct gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttca
    36001 aaaccagaga tcgcatcgcc ggtgccgcct tcggccttca ctgcgttggt catgtaatag
    36061 ctgaaaccaa gataatcgca ggtgccttcg cgcagtacat cgagatcgcc gtcttccatt
    36121 ttgatattaa atccgcgacg ttcccactcg ttcaatacat aggacgggta atagccgcat
    36181 agctgcacat cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg
    36241 tccgggttac atgagtaggg atagagcggc accatcgcca gcatacagcc gactttcatc
    36301 tccgggttaa tgcgacgcgc ggctttcact gccagggcgc tggcgacaaa ctggtgatgc
    36361 agcacctggt acatcgtctc ttccgggttg tcatgctcgg tatacaccac gccggagcag
    36421 cagtaaccaa acagcggcgc acgccagtta cgctggttgt ttatttcgtt gaaggtcatc
    36481 cagtatttga ctttatgctt gtagcgttcg aataccactt ccgcgaaacg gacaaagaaa
    36541 tcaacgaatt tacggttggt ccagctaccg tattgctgca ccagatgcag cggcatttca
    36601 aagtgggaga gggttatcac cggttcgatg ttgtatttca gcagttcatc gaacagagag
    36661 tcgtaaaact tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc
    36721 gtccaggcga tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct
    36781 tccttgtagt gaccataaaa atcaacggct tcatggtttg gatagtattt tcctggcaag
    36841 acttctttgg tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg
    36901 ctcggtcctt tgccgccttt gttccagccg ccttcgacct gatgagcggc aacggcgcca
    36961 ccccataaga aatctttcgg taaggtgagt tttttcacta tcataggctc cagatcagtc
    37021 aattcttaca ggaaatagta ccatgcagcc aaacgacatc acttttttca acgtttccag
    37081 gatgacattc tggctgggcg taaaaccatc accatccgcg acgagtctga atcgcacttc
    37141 aaaacgggtg atgtgcttcg tgtcggacgt tttgaagatg acggttattt ttgcacgatt
    37201 gaagtcaccg caacctcaac cgtaacgctg gatacactga cggaaaaaca tgcagagcag
    37261 gaaaatatga ccctgcctga actgaaaaag gtcattgctg acatctatcc cggtcagaca
    37321 caattttata tgattgaatt taaatgtctt taaattgaac tcacgcctgt tagttgaaat
    37381 ttcgcattta atttcatgat ttttaggaga ttagtctcaa atcagaattt attttagcta
    37441 acaggtgttc actggaacta ttctcagtta cgctggagag gtatacatgt gcgtgtacgt
    37501 ttctctggag taagttatgg ttcagaagcc cctcattaag cagggatatt cgctggcaga
    37561 ggaaattgcc aacagcgtca gtcacggcat tgggctggtg tttggtatcg ttgggctggt
    37621 gttgctactg gttcaggcgg tggatctcaa tgccagcgcc acagcgataa ccagttacag
    37681 cctctatggc ggcagtatga tcctgctgtt cctcgcttcg acgctctatc acgccattcc
    37741 tcatcaacgg gcaaaaatgt ggctgaagaa atttgaccat tgcgctattt acctgttgat
    37801 tgccggaacc tacacgccgt ttttgctggt ggggctggat tctccgttag cgcgcgggtt
    37861 gatgattgtt atctggagcc tagcattgct ggggattctg ttcaaactga ccatcgcgca
    37921 ccgattcaaa attttatctc tggtgactta tctggcgatg ggctggctgt cgctggtggt
    37981 aatttatgaa atggcagtta agctcgcggc gggcagcgtt accttactgg cggtaggcgg
    38041 cgtggtttat tcgctcgggg tgattttcta cgtctgcaaa cgcattccat acaaccatgc
    38101 catctggcac ggcttcgtgc tcggcggtag tgtgtgccac tttctggcga tctatttgta
    38161 tattgggcag gcgtaatggg tatgcctttg taggcctgat aagacgcgtc agcgtcgcat
    38221 caggcatcgg agcgcaaccg ccggatgcgg cgtgaacgcc ttatccgatc tacgggcctc
    38281 gtccctgttg tgataaaggc atcaaatccc agacatgata agacgcgtca gcgtcgcatc
    38341 aggcgataaa tgcctgatgc gatacagaga tttactcttc gagcgaatac ggcagcggct
    38401 cgatatgcag tgtattcgca tcgtcgcgta cgcggaagat gctgtccggt tccatatcgt
    38461 tattcatgac gacctgtacc acgacctgac catcttccag ttttaccgca gccagcaccg
    38521 taccggtacg acgccagttc tcgcccattt tcagctctaa gtcttcacca gcttccggca
    38581 gtcggctggc actacctgcc agcaaccaga gcgcgcgttt gttggcacca cggaattttg
    38641 ctcgcgccac catctcttgt ccggtataac agcctttctt aaagctgata ccgcccagcg
    38701 cctggagatt ggtcgcctgt gggataaact gcccgctgtt ggcggcatca atcaccggga
    38761 aaccagcttc aatgtttaat gccagccact gttggctatt gttcagttcc gcttcaccgc
    38821 gcagtttatc ggtcagcata ttagcagtag cttcatcggt tacgatcagg aaacgttctg
    38881 ccgggtgttc aaaccatagc aaagtggtcg cgccttcttt gactacctgt ttttctttcg
    38941 aaggcagttc gctaaagaga tttgccagcg cggcgcgcgc ctgaaaaccg gcaacaccaa
    39001 gcagcacacg ctcgtcgtct ggcgcgatgg tcactttaga gaataccgca tattttttca
    39061 gttcagtcag ctgcggttca cgcacgctgc gccgttcaat ccatgcaaag ccatcgccgt
    39121 cgcggaacag acgtaaattg ctccacattt tacctttggc gtcgcaatgg gcggcgagca
    39181 ggtgctgatc ttctgccatc tggctgacat ctgctgtcac ctgaccctgc atatattttt
    39241 cgctgtccgc gccagtaatg gtggcaagcg cccagtcatc aagcgtcatc agcgtcagcg
    39301 gtaaacgggc agaagccgta ggctgacgtg gaggaaaagg tgtaaaagcc ataatagagt
    39361 cctgagttgc ttaacggcgt attaatgact aatggtaaaa gagctatgag gcaatgcaag
    39421 tgctttcact acgcgatctg tgccttctgc gaggcgactg ccaaaaaaat gaagcccttg
    39481 aattgttgtg aattaatttc gcgatcgcga tggtgtttac tgatattccc gtaaaagaag
    39541 tcgaaaaaca cgttacaata gccgggtact ggattttcgt gagacaggaa gaagatggac
    39601 attaacaaca aagcccgaat tcattgggca tgccgccgtg gtatgcgcga actcgatatt
    39661 tcaatcatgc cgtttttcga acatgagtac gacagcttaa gcgatgacga aaaacgcatc
    39721 tttattcgtc tgctggaatg tgacgatccg gacctgttta actggctgat gaatcacggt
    39781 aaaccggccg atgcagaact ggaaataatg gtccgactca tccagacacg gaaccgggaa
    39841 cgtggtcctg tggcaatctg atttgcgcgt ctcctggcgc gcacagtggc tttccttgct
    39901 gattcatggg ctggttgccg ctgttatttt actcatgccc tggccgctca gttacacccc
    39961 gttatggatg gtgttacttt cgctggtggt atttgattgc gttcgcagcc agcggtgtat
    40021 taatgctcgc cagggggaaa ttcgcttgtt gatggacggg cgtttgcgtt ggcaagggca
    40081 ggagtggagc atcgtcaaag caccgtggat gattaagagc ggcatgatgc tgcgtttacg
    40141 ttctgatggc ggtaaacggc aacatttatg gctggcagcc gacagcatgg acgaagccga
    40201 atggcgggat ttacggcgga ttttgttgca acaagagacg caaagataaa gatgacggca
    40261 aagcatatgc gctaagccgt cagtgagatc aggcgtaatg ctctgccatt tcgttgagga
    40321 tttgctcgca ccagctctga atacgctcgt cgctaaggtc atactggtta gtttcatcca
    40381 gcgccagacc cacgaacagt tgcccgtcag caatcaccgg tttcgggctg gtaaattcat
    40441 atccttccgt tggccagtag ccgacgaact tcacgccttt ggtcgagagt ttgtcatgca
    40501 gcataccgag cgcatcgagg aaccactcgc cgtatcccag ttgatcgcca agcccataca
    40561 gcgcaataat tttaccttca aggttcaggt cgtcgagctg atcccagacg gcttcccagt
    40621 cttcctggat ttcaccaaaa tcccaggtcg ggatacccag aatgagcaca tcgtactgct
    40681 ccattaattt cggggagtcg tccttgaggt tatgtaaggt caccagttct gggccgataa
    40741 tatcgcggat tttttctgcc gccatttcgg tgtaacaggt gctggaaccg taaaaaagac
    40801 ccatattcat aacgtaagct tctcgattct gctgtggctt ttgcagctag tgtatcagaa
    40861 tcgctttagt cacggcacaa taaggcataa tggatcaaat gagtgaccat aaggggcgca
    40921 agtgaaacag gagctggcac gcatcgagca gtttcttgat gctctgtggc tggaaaaaaa
    40981 tctggctgaa aatacgttga acgcttaccg tcgcgatctg tcaatgatgg tggagtggtt
    41041 gcatcaccgc gggttgacgc tggcgacggc gcaaagtgac gatttgcagg cattactggc
    41101 agaacggctg gagggcgggt ataaagccac cagctcagcg cgtttgctga gtgcagtgcg
    41161 ccgattgttc cagtatcttt atcgcgaaaa gtttcgtgaa gacgatccca gtgcgcatct
    41221 cgcttcaccg aaattgcccc agcgtttgcc aaaagattta agcgaagcgc aggtcgaacg
    41281 tttattacag gcgccattaa ttgatcagcc actggagcta cgcgataaag ccatgcttga
    41341 agtgttgtat gctaccgggc tgcgtgtctc tgaactggtc gggctgacaa tgagtgatat
    41401 cagcctgcgt cagggcgtag tgcgggtcat tggtaaaggc aacaaagagc gcctggtgcc
    41461 gttaggggaa gaggcggttt actggctgga aacctatctg gaacatgggc gtccgtggct
    41521 gctgaatggt gtgtcaattg acgtgttgtt tcccagccag cgtgcgcagc agatgacgcg
    41581 acagaccttc tggcaccgca ttaaacatta tgccgtgctg gcgggtatcg acagcgaaaa
    41641 gctgtcaccg catgtgttgc gtcacgcttt tgccactcat ttattaaatc atggtgcgga
    41701 tttacgcgtg gtgcagatgc tactgggcca cagcgatctt tccaccacgc aaatttatac
    41761 gcatgtcgct accgagcgtc tgcggcaact tcatcaacag catcacccgc gggcgtgatg
    41821 tctgaaaaga acgggaagat ttatgaagaa aggttttatg ttgtttactt tgttagcggc
    41881 gttttcaggc tttgctcagg ctgatgacgc ggcaattcaa caaacgttag ccaaaatggg
    41941 catcaaaagc agcgatattc agcccgcgcc cgtagctggc atgaagacag ttctgactaa
    42001 cagcggtgtg ttgtacatca ccgatgatgg taaacatatc attcaggggc caatgtatga
    42061 cgttagtggc acggctccgg tcaatgtcac caataagatg ctgttaaagc agttgaatgc
    42121 gcttgaaaaa gagatgatcg tttataaagc gccgcaggaa aaacacgtca tcaccgtgtt
    42181 tactgatatt acctgtggtt actgccacaa actgcatgag caaatggcag actataacgc
    42241 gctggggatc accgtgcgtt atcttgcttt cccgcgccag gggctggaca gcgatgcaga
    42301 gaaagaaatg aaagctatct ggtgtgcgaa agataaaaac aaagcgtttg atgatgtgat
    42361 ggcaggtaaa agcgtcgcac cagccagctg cgacgtggat attgccgacc attacgcact
    42421 tggcgtccag cttggcgtta gcggtactcc ggcagttgtg ctgagcaatg gcacacttgt
    42481 tccgggttac cagccgccga aagacatgaa agaattcctc gacgaacacc aaaaaatgac
    42541 cagcggtaaa taattcgcgt gaaacaacag atacaacttc gtcgccgtga agtcgatgaa
    42601 acggcagact tgcccgctga attgcctccc ttgctgcgcc gtttatacgc cagccgggga
    42661 gtacgcagtg cgcaagaact ggaacgcagt gttaaaggta tgttgccctg gcagcaacta
    42721 agcggtgtcg aaaaggccgt tgagatcctt tacaacgctt ttcgcgaagg aacgcggatt
    42781 attgtggtcg gtgatttcga cgccgacggc gcgaccagca cggctctaag cgtgctggcg
    42841 atgcgctcgc ttggttgcag caatatcgac tacctggtac caaaccgttt cgaagacggt
    42901 tacggcttaa gcccggaagt ggtcgatcag gcccatgccc gtggcgcgca gttaattgtc
    42961 acggtggata acggtatttc ctcccatgcg ggagttgagc acgctcgctc gttgggcatc
    43021 ccggttattg ttaccgatca ccatttgccg ggcgacacat tacccgcagc ggaagcgatc
    43081 attaacccta acttgcgcga ctgtaatttc ccgtcgaaat cactggcagg cgtgggtgtg
    43141 gcgttttatc tgatgctggc gctgcgcacc tttttgcgcg atcagggctg gtttgatgag
    43201 cgcggcatcg caattcctaa cctggcagaa ctgctggatc tggtagcact ggggacagta
    43261 gcggacgtcg tgccgctgga cgctaataat cgcattctga cctggcaggg gatgagtcgc
    43321 atccgtgccg gaaagtgccg tccagggatt aaagcgctgc tggaagtggc aaatcgcgat
    43381 gcacaaaaac tagccgccag cgatttaggt tttgcgctgg gaccgcgtct caatgctgcc
    43441 gggcgactgg atgatatgtc cgttggtgtg gcgctgttgc tgtgcgacaa catcggcgaa
    43501 gcgcgcgtgc tggcaaatga actcgatgcg ctaaaccaga cgcgaaaaga gatcgaacaa
    43561 ggaatgcagg ttgaagccct gaccctgtgc gagaaactgg agcgcagccg tgacacgcta
    43621 ctcggcgggc tggcaatgta tcaccccgaa tggcatcagg gcgttgtcgg tattctggct
    43681 tcgcgtatca aagagcgttt tcaccgtccg gttatcgcct ttgcgccagc aggtgacggt
    43741 acgctgaaag gttccggtcg ctccattcag gggctgcata tgcgtgatgc gctggagcga
    43801 ttagacacac tctaccccgg catgatgctc aagtttggcg gtcatgcgat ggcggcgggt
    43861 ttgtcgctgg aagaggataa attcgaactc tttcaacaac ggtttggcga actggttact
    43921 gagtggctgg ccccttcgct attgcaaggc gaagtggtgt cagacggtcc gttaagcccg
    43981 gccgaaatga ccatggaagt ggcgcagctg ctgcgcgatg ctggcccgtg ggggcagatg
    44041 ttcccggagc cgctgtttga cggtcatttc cgtctgctgc aacagcggct ggtgggcgaa
    44101 cgtcatttga aggtgatggt cgaaccggtc ggcggcggtc cactgctgga tggtattgct
    44161 tttaatgtcg ataccgctct ctggccggat aacggcgtgc gcgaagtgca actggcttat
    44221 aagctcgata tcaacgagtt tcgcggcaac cgcagcctgc aaattatcat cgacaatatc
    44281 tggccaattt agcgtcatct tctctataaa aaagagcgtg gattgggtac aatcccgctc
    44341 ttatcaccgc attttgacta gctcaataaa agaaatcaga ccatgtttga aattaatccg
    44401 gtaaataatc gcattcagga cctcacggaa cgctccgacg ttcttagggg gtatctttga
    44461 ctacgacgcc aagaaagagc gtctggaaga agtaaacgcc gagctggaac agccggatgt
    44521 ctggaacgaa cccgaacgcg cacaggcgct gggtaaagag cgttcctccc tcgaagccgt
    44581 tgtcgacacc ctcgaccaaa tgaaacaggg gctggaagat gtttctggtc tgctggaact
    44641 ggctgtagaa gctgacgacg aagaaacctt taacgaagcc gttgctgaac tcgacgccct
    44701 ggaagaaaaa ctggcgcagc ttgagttccg ccgtatgttc tctggcgaat atgacagcgc
    44761 cgactgttac ctcgatattc aggcggggtc tggcggtacg gaagcacagg actgggcgag
    44821 catgcttgag cgtatgtatc tgcgctgggc agaatcgcgt ggtttcaaaa ctgaaatcat
    44881 cgaagagtcg gaaggtgaag tggcgggtat taaatccgtg acgatcaaaa tctccggcga
    44941 ttacgcttac ggctggctgc gtacagaaac tggcgttcac cgcctggtgc gtaaaagccc
    45001 gtttgactcc ggcggtcgtc gccacacgtc gttcagctct gcgtttgttt atccggaagt
    45061 tgatgatgat attgatatcg aaatcaaccc ggcggatctg cgcattgacg tttatcgcgc
    45121 gtccggcgcg ggcggtcagc acgttaaccg taccgaatct gcggtgcgta ttacccacat
    45181 cccgaccggg atcgtgaccc agtgccagaa cgaccgttcc cagcacaaga acaaagatca
    45241 ggccatgaag cagatgaaag cgaagcttta tgaactggag atgcagaaga aaaatgccga
    45301 gaaacaggcg atggaagata acaaatctga catcggctgg ggcagccaga ttcgttctta
    45361 tgtccttgat gactcccgca ttaaagatct gcgcaccggg gtagaaaccc gcaacacgca
    45421 ggccgtgctg gacggcagcc tggatcaatt tatcgaagca agtttgaaag cagggttatg
    45481 aggaaccaac atgtctgaac aacacgcaca gggcgctgac gcggtagtcg atcttaacaa
    45541 tgaactgaaa acgcgtcgtg agaagctggc gaacctgcgt gagcagggaa ttgccttccc
    45601 gaacgatttc cgtcgcgatc atacctctga ccaattgcac gcagaattcg acggtaaaga
    45661 gaacgaagaa ctggaagcgc tgaacatcga agtcgccgtt gctggccgca tgatgacccg
    45721 ccgtattatg ggtaaagcgt ctttcgttac cctgcaggat gtgggtggcc gcattcagct
    45781 gtacgttgcc cgtgacgatc tgccggaagg cgtttacaac gagcagttca aaaaatggga
    45841 cctcggcgac atcctcggcg cgaaaggtaa actgttcaaa accaaaaccg gcgaactgtc
    45901 tatccactgt accgagctgc gtctgctgac caaagcactg cgtccgctgc cggataaatt
    45961 ccacggcttg caggatcagg aagcgcgcta tcgtcagcgt tatctggatc tcatctctaa
    46021 cgatgaatcc cgtaacacct ttaaagtacg ctcgcagatc ctctctggta ttcgccagtt
    46081 catggtgaac cgcggcttta tggaagttga aacgccgatg atgcaggtga tcccaggcgg
    46141 tgccgctgcg cgtccgttta tcacccacca taacgcgctg gatctcgaca tgtacctgcg
    46201 tatcgcgccg gaactgtacc tcaagcgtct ggtggtcggt ggcttcgagc gtgtattcga
    46261 aatcaaccgt aacttccgta acgaaggtat ttccgtgcgt cataacccag agttcaccat
    46321 gatggaactc tacatggctt acgcagatta caaagatctg atcgagctga ccgaatcgct
    46381 gttccgtact ctggcacagg atattctcgg taagacggaa gtgacctacg gcgacgtgac
    46441 gctggacttc ggtaaaccgt tcgaaaaact gaccatgcgt gaagcgatca agaaatatcg
    46501 cccggaaacc gacatggcgg atctggataa cttcgactct gcgaaagcga ttgctgaatc
    46561 tatcggtatc cacgttgaga agagctgggg tctgggccgt atcgttaccg agatcttcga
    46621 agaagtggca gaagcacatc tgattcagcc gaccttcatt actgaatatc cggcagaagt
    46681 ttctccgctg gcgcgtcgta acgacattaa cccggaaatc acagaccgct ttgagttctt
    46741 catcggtggt cgtgaaatcg gtaacggctt tagcgagctg aatgacgcgg aagatcaggc
    46801 gcaacgcttc ctggatcagg ttgccgcgaa agacgcaggt gacgacgaag cgatgttcta
    46861 cgatgaagat tacgttaccg cactggaaca tggcttaccg ccgacagcag gtctggggat
    46921 tggcatcgac cgtatggtaa tgctgttcac caacagccat accatccgcg acgttattct
    46981 gttcccggcg atgcgtccgg tgaaataagc attacgttat gctcacaacc ccggcaaatg
    47041 tcggggtttt ttatttaagc tgggtaaatg cagataatcg tattctggct tcgcgatttg
    47101 tcgcctgcat caccatccac ggactgaacg cccacggcgt ggcatcaata ccgcgtaata
    47161 catcagctaa atcacaccat tgataatcca tcacttcatc atcgttgatc tgtaacgcac
    47221 tggtggtgcg tgcggcaaat accggacaca cttcattttc cacaatgcct ctcggatcgg
    47281 tggcgcggta gcgaaagtca ggatagatag attcaggagg cgtaatttcc acgccaagct
    47341 cataacggca acggcggatc accgcgtctt cgctgctttc tcccagttgt gggtgcccac
    47401 aaaccgagtt agtccacacg ccaggccacg cttttttgct cagtgcgcgg cgggtaacta
    47461 ataattgccc cttggcatta aacagccaac tggagaacgc gagatgtaag agggtgtctg
    47521 ccgtgtgtgc ggcatacttt tccagcgtac ccgtgggaac tccctgtgca ttcaataaaa
    47581 tgacgtgttc cgtttgcata atttctcaca tgtaattctg atcactcatc attatagata
    47641 attgaagagt gaaatgatta accaagagag cgtttatcga caaccgcccg ttttgagcgg
    47701 atgccgacag gtaaaaacca tttaaaaaag tagcatcata tatttgaagc gactttcaat
    47761 ggccattgca acacagtcag aaatgacagg aatagagaga gtaaaaagca acaacagact
    47821 gaatatagcg cgcattaaaa aattcatatt attctccatg ctcatttttc ttcagcttgc
    47881 gcatcagaag ccacatacgt actgtcctga cttctaaatc tttatcagag acggattcct
    47941 tcaccaggtc agcatgatgc tgatagccat taaagaagac gttcagcaca acggcgctaa
    48001 gggttgcgag cataatgccg ctatgtagca gcggttgcag tacggccggt aacttagaaa
    48061 agaaatcgtg agagagcgtc ggagtcatgc caacgccgag actgatcgcc acaatataga
    48121 ggttgtaacg gttggtggtg tagttacagc gcgacagaat tcgaatccct gtcgccagta
    48181 ccatgccgaa catcactaga ccagcgccgc ccagcacaaa ttgcggaatg gaggctacca
    48241 gcaccgccat ttttggcacc atgccaaaca ggattaatat aattcccgaa gaaatacaca
    48301 cccagcggct atgaacgcgc atcacgctaa ccaggccaac gttttgagaa aatgacgtgt
    48361 gggggaagct gttaaacgtg ccgcctatca ttgtccctac gccatcgaca cgcagcccgc
    48421 aaataatatc gtgtgaagag agtttacgac cgactatttc acccagtgcc aggaacatcc
    48481 ccatcgactc gataaacacg atgattaaca cggcagtcat ggtcagaatg gaaacgggat
    48541 cgaaaatcgg cataccaaac gacatcgggg tgacaatcgc aaaccacgag gcatcatgta
    48601 gcccggataa attgacttca ttcatcatcc acgaaagtaa aaagccaaat acaatcccca
    48661 gtaatacggc gacgttggac ataaatcctt tcgcatagcg agtaatgagc aagataaaaa
    48721 ttaagacggc aaaggagata cctaaataaa cgggattacc atattgcgga ttccctttac
    48781 ctccagcggc ccagtcaata cccacctgaa tgatgctaag cccaatagaa gtaataacca
    48841 caccggtaac cagtggcggg aataaaggca tcaagcgacc aataagtggc gctaataatg
    48901 tggtgataaa acccgcggcg atagtggcac caaatatccc cagcaggccg atatccgggt
    48961 tcatcccaat ggctatcatt ggtgttacag cagcaaaggt caccgacata atcaccggca
    49021 ggcggatccc cataaagcgg ccgataccga tacattgcaa taatgtgacg atcccgcagc
    49081 aaaagagatc cgagctaatg agcatcgcaa tagcatcttt tgagaggccc agtcggtcac
    49141 caatcataag aggaacagcg actgcacctg cgtacatgac aaggacgtgt tgcagaccga
    49201 gtatgatcag ctttcctggt gataatatgc ggtcaacctc atcagttggg cggtcttgcc
    49261 cagaagatga gggaagttgg gaatctatgg cgctcatcag tgtttctcct ttcgcccgcc
    49321 agaataaaaa gcaaccgttt acgttaatga aactgtctaa acacatgctc gtggagtgcc
    49381 agtttttgcg aggaagggga gttagagaca ggaattgcgg aggcaatcac ataatcgtgc
    49441 ttaaaccacg aaaatgacaa gagaacctga caatggaatt atctgtaatt ttctcatatt
    49501 gatatttcat taatcattct ggcatgtcgc actctcgcat ttaatcgttt ttatctggat
    49561 agcgctcttt tgatcggcaa tgttgaaata atgaaagcgg catagattga ggggcaataa
    49621 tttgaactca gtcgcaaaat tataattaaa acggtcatgc tacctaatat atgcccgttt
    49681 attgcatcgc gaaatttgag aagacataac tcattaaatg aaagggatgc aaatgaataa
    49741 gtttatcgct gctgaagctg cggaatgtat aggctgtca
//
LOCUS       DAJTRB010000019        49508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTRB010000019 DAJTRB010000000
VERSION     DAJTRB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.019"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..1140)
                     /gene="setB"
                     /locus_tag="OO330_001189"
     CDS             complement(<1..1140)
                     /gene="setB"
                     /locus_tag="OO330_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5882130.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMI"
     gene            1508..2638
                     /gene="fruB"
                     /locus_tag="OO330_001190"
     CDS             1508..2638
                     /gene="fruB"
                     /locus_tag="OO330_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5882131.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2638..3576
                     /gene="fruK"
                     /locus_tag="OO330_001191"
     CDS             2638..3576
                     /gene="fruK"
                     /locus_tag="OO330_001191"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5882132.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3593..5284
                     /gene="fruA"
                     /locus_tag="OO330_001192"
     CDS             3593..5284
                     /gene="fruA"
                     /locus_tag="OO330_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5882133.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5708..6649
                     /locus_tag="OO330_001193"
     CDS             5708..6649
                     /locus_tag="OO330_001193"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5882134.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6637..7575
                     /gene="psuG"
                     /locus_tag="OO330_001194"
     CDS             6637..7575
                     /gene="psuG"
                     /locus_tag="OO330_001194"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5882135.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7669..8919
                     /locus_tag="OO330_001195"
     CDS             7669..8919
                     /locus_tag="OO330_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5882136.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8990..9684)
                     /gene="yeiL"
                     /locus_tag="OO330_001196"
                     /pseudo
     CDS             complement(8990..9684)
                     /gene="yeiL"
                     /locus_tag="OO330_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9815..10756
                     /gene="rihB"
                     /locus_tag="OO330_001197"
     CDS             9815..10756
                     /gene="rihB"
                     /locus_tag="OO330_001197"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5882137.1"
                     /translation="MEKREIILDCEPGHDDAIAMMMAAKHSAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10856..12106
                     /locus_tag="OO330_001198"
     CDS             10856..12106
                     /locus_tag="OO330_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5882138.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTAQVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAILGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIARLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12162..13250)
                     /locus_tag="OO330_001199"
     CDS             complement(12162..13250)
                     /locus_tag="OO330_001199"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5882139.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLSELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13253..14110)
                     /gene="nfo"
                     /locus_tag="OO330_001200"
     CDS             complement(13253..14110)
                     /gene="nfo"
                     /locus_tag="OO330_001200"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5882140.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRIDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14184..15233)
                     /locus_tag="OO330_001201"
     CDS             complement(14184..15233)
                     /locus_tag="OO330_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5882141.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15332..16213
                     /locus_tag="OO330_001202"
     CDS             15332..16213
                     /locus_tag="OO330_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5882142.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16419..17888
                     /gene="lysP"
                     /locus_tag="OO330_001203"
     CDS             16419..17888
                     /gene="lysP"
                     /locus_tag="OO330_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5882143.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18180..20171
                     /gene="cirA"
                     /locus_tag="OO330_001204"
     CDS             18180..20171
                     /gene="cirA"
                     /locus_tag="OO330_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR5882144.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSDTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20203..21038)
                     /gene="yeiG"
                     /locus_tag="OO330_001205"
                     /pseudo
     CDS             complement(20203..21038)
                     /gene="yeiG"
                     /locus_tag="OO330_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21296..21964
                     /gene="folE"
                     /locus_tag="OO330_001206"
     CDS             21296..21964
                     /gene="folE"
                     /locus_tag="OO330_001206"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5882145.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21981..23057
                     /locus_tag="OO330_001207"
     CDS             21981..23057
                     /locus_tag="OO330_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5882146.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23199..24239
                     /gene="galS"
                     /locus_tag="OO330_001208"
     CDS             23199..24239
                     /gene="galS"
                     /locus_tag="OO330_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5882147.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24519..25517
                     /gene="mglB"
                     /locus_tag="OO330_001209"
     CDS             24519..25517
                     /gene="mglB"
                     /locus_tag="OO330_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5882148.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25578..27098
                     /gene="mglA"
                     /locus_tag="OO330_001210"
     CDS             25578..27098
                     /gene="mglA"
                     /locus_tag="OO330_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5882149.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27114..28124
                     /gene="mglC"
                     /locus_tag="OO330_001211"
     CDS             27114..28124
                     /gene="mglC"
                     /locus_tag="OO330_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5882150.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28160..28510)
                     /locus_tag="OO330_001212"
                     /pseudo
     CDS             complement(28160..28510)
                     /locus_tag="OO330_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(28600..29028)
                     /locus_tag="OO330_001213"
     CDS             complement(28600..29028)
                     /locus_tag="OO330_001213"
                     /inference="COORDINATES: protein motif:HMM:NF013355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5882151.1"
                     /translation="MGAAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYCRAVKRG
                     STLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKS
                     SISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIET"
     gene            complement(29004..29372)
                     /locus_tag="OO330_001214"
     CDS             complement(29004..29372)
                     /locus_tag="OO330_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR5882152.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEQLARI"
     gene            complement(29366..30604)
                     /gene="preT"
                     /locus_tag="OO330_001215"
     CDS             complement(29366..30604)
                     /gene="preT"
                     /locus_tag="OO330_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5882153.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGGKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30798..31037)
                     /locus_tag="OO330_001216"
     CDS             complement(30798..31037)
                     /locus_tag="OO330_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5882154.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31040..31759)
                     /gene="sanA"
                     /locus_tag="OO330_001217"
     CDS             complement(31040..31759)
                     /gene="sanA"
                     /locus_tag="OO330_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5882155.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31909..32793)
                     /gene="cdd"
                     /locus_tag="OO330_001218"
     CDS             complement(31909..32793)
                     /gene="cdd"
                     /locus_tag="OO330_001218"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5882156.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32923..33618)
                     /locus_tag="OO330_001219"
     CDS             complement(32923..33618)
                     /locus_tag="OO330_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5882157.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33615..34013)
                     /locus_tag="OO330_001220"
     CDS             complement(33615..34013)
                     /locus_tag="OO330_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5882158.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34252..35199
                     /gene="dusC"
                     /locus_tag="OO330_001221"
     CDS             34252..35199
                     /gene="dusC"
                     /locus_tag="OO330_001221"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5882159.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35570..35653)
                     /gene="yohP"
                     /locus_tag="OO330_001222"
     CDS             complement(35570..35653)
                     /gene="yohP"
                     /locus_tag="OO330_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5882160.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35877..37313
                     /gene="mdtQ"
                     /locus_tag="OO330_001223"
     CDS             35877..37313
                     /gene="mdtQ"
                     /locus_tag="OO330_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR5882161.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37366..38127
                     /locus_tag="OO330_001224"
                     /pseudo
     CDS             37366..38127
                     /locus_tag="OO330_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38244..38822)
                     /locus_tag="OO330_001225"
     CDS             complement(38244..38822)
                     /locus_tag="OO330_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5882162.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            38992..39579
                     /locus_tag="OO330_001226"
     CDS             38992..39579
                     /locus_tag="OO330_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5882163.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39753..40685
                     /gene="pbpG"
                     /locus_tag="OO330_001227"
     CDS             39753..40685
                     /gene="pbpG"
                     /locus_tag="OO330_001227"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5882164.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40723..42438)
                     /gene="dld"
                     /locus_tag="OO330_001228"
     CDS             complement(40723..42438)
                     /gene="dld"
                     /locus_tag="OO330_001228"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5882165.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42634..44931
                     /gene="bglX"
                     /locus_tag="OO330_001229"
     CDS             42634..44931
                     /gene="bglX"
                     /locus_tag="OO330_001229"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5882166.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45183..46100
                     /gene="osmF"
                     /locus_tag="OO330_001230"
     CDS             45183..46100
                     /gene="osmF"
                     /locus_tag="OO330_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5882167.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46107..47264
                     /gene="yehY"
                     /locus_tag="OO330_001231"
     CDS             46107..47264
                     /gene="yehY"
                     /locus_tag="OO330_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5882168.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47257..48183
                     /gene="yehX"
                     /locus_tag="OO330_001232"
     CDS             47257..48183
                     /gene="yehX"
                     /locus_tag="OO330_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5882169.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48188..48919
                     /gene="yehW"
                     /locus_tag="OO330_001233"
     CDS             48188..48919
                     /gene="yehW"
                     /locus_tag="OO330_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5882170.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48900..49007)
                     /locus_tag="OO330_001234"
     CDS             complement(48900..49007)
                     /locus_tag="OO330_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5882171.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49067..49508)
                     /locus_tag="OO330_001235"
                     /pseudo
     CDS             complement(49067..49508)
                     /locus_tag="OO330_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11949 a        12199 c        13301 g        12059 t
ORIGIN      
        1 aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg cgacgatgcc
       61 cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga gcgtggtggc
      121 tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc cgcccagaat
      181 gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg aatgcgccat
      241 cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta agaaacgctt
      301 acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca gcccggcggc
      361 ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga taataaatag
      421 cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa tgacaaacag
      481 cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg ccagcggaag
      541 ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa taaacgctac
      601 cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat aagccagcgg
      661 tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga acatcaccgc
      721 ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg ggttagcggt
      781 cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt tgcgattcca
      841 agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca gcgatttgcg
      901 atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc caatgacagc
      961 gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat cggtaaggaa
     1021 aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg caacgattaa
     1081 aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg agttatgcat
     1141 gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag aaaataaaca
     1201 gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt gctgattggc
     1261 tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca attaggaaaa
     1321 atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc tttccttgcg
     1381 tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg tctttaaccc
     1441 taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc agcaagagag
     1501 gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg ccggagacaa
     1561 agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg tagcagaagg
     1621 ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg gcaatggtat
     1681 tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg gcgttcaggt
     1741 atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg tggcaatcgg
     1801 tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc acgtactgag
     1861 cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac ttcgcgcatt
     1921 actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc tgacgctgga
     1981 tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc tgaaagaggc
     2041 gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc tgaatctcgg
     2101 acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga ttgcggtaag
     2161 ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg tgagtgtggc
     2221 gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt tgctcgacaa
     2281 taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc tgaccagcga
     2341 tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg aacacggcct
     2401 gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca gtgatattac
     2461 cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga tgaaagttgt
     2521 ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg cagatgctga
     2581 acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg gcgcataatg
     2641 agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg tttctgcccg
     2701 gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc ggcgggtaaa
     2761 ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt tggcggcttc
     2821 ctaggtaaag acaatcagga tggttttcag caactgttca gcgagctggg cattgccaac
     2881 cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga aaaagacggc
     2941 gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg ggaacgcttt
     3001 gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag cggaagctta
     3061 ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg tagtcagtgt
     3121 ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa agcggcaccg
     3181 tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa actgcctgaa
     3241 atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc gcatgttgtt
     3301 atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg gatcgctaaa
     3361 ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt tggtggcctg
     3421 atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc gacagctgtt
     3481 gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca gttggccgca
     3541 atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca taatgaaaac
     3601 gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga agaccctgct
     3661 gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg acgcagaaat
     3721 ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta aaaatgtctg
     3781 gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg aagccaaagg
     3841 ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg ccagcggtcc
     3901 gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct ttatggcggc
     3961 tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg aaacccgtgg
     4021 ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg atctggtgat
     4081 tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga tgtatcgtac
     4141 ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg ttgctgaagc
     4201 aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta agaaagagag
     4261 tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga tggtcgttgc
     4321 tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta aagagccggg
     4381 tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc tgatggtgcc
     4441 ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca cgccgggtct
     4501 gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta ttattgcggg
     4561 cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc cgcagagtat
     4621 ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg tcggtctggc
     4681 gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga ctcactggct
     4741 gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg gcatgatgtg
     4801 tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg gtctgctgag
     4861 tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc caccgctggc
     4921 aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc aggaaggtgg
     4981 caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc cgtttgctgc
     5041 tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga ctggcgcaat
     5101 ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg ttctgctgat
     5161 ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg gtacgctggt
     5221 ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag cgaaagcagc
     5281 gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg atgagcgcaa
     5341 agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat ctttcagtag
     5401 aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt attttctgcc
     5461 cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt gtaattcagg
     5521 catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc tggcggtaat
     5581 ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca catgtttaaa
     5641 ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt ttaccagagg
     5701 acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata ttgatgtcgc
     5761 cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa taaaatttac
     5821 gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta acaaagcctg
     5881 gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc aaaccaatca
     5941 atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt cgagttattt
     6001 atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga atattagcaa
     6061 cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg caaaggtcat
     6121 tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata atgccgccaa
     6181 cgtacccgta tttgtcgatc cggtttcagc gcggaaatgt gtcaaagtgc gcgaccgtct
     6241 aaatcagatc cacactctga agccaaaccg ccttgaagcg gaaaccctga gtgggattgc
     6301 gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac atggcctgaa
     6361 ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca gcggtgaaaa
     6421 tagctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg gcgatgccat
     6481 gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat ctgttcgttt
     6541 cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc ccgatttatc
     6601 gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa aatctcccct
     6661 gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa accggttgtg
     6721 gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc ccagaccgca
     6781 attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat cgccattatt
     6841 ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg tcgtgaaggg
     6901 cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc cggaaaaaat
     6961 ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat taaagtattt
     7021 gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga tatttctgcc
     7081 gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc gaaatctatt
     7141 ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt aattggctat
     7201 cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt cagcattcgt
     7261 ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag cgggctgaac
     7321 ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga ggaatctatc
     7381 aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat tggtaaagaa
     7441 agtacaccgt tcctgctggc gcgcgttgct gagctgaccg gcggtgacag cctgaaatcc
     7501 aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa agaataccag
     7561 cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat caagcatacc
     7621 aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat ggatataatg
     7681 agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc agtgaataaa
     7741 aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc tattggtggc
     7801 atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt aggcgttcat
     7861 aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct ggttgggccg
     7921 aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt acttccggcg
     7981 attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat ggggctgctg
     8041 attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat cgaatctttt
     8101 gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt taaaccgttt
     8161 atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat ggcgtccatt
     8221 gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta cctgttagcg
     8281 gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag cccggcaacc
     8341 gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc aaaaagcttt
     8401 atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg tgtagcgacg
     8461 gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg cggaattggc
     8521 ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt gctggcaccg
     8581 ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgcaggtag cctgattggg
     8641 cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata cctgcaaacg
     8701 ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg tggttttgct
     8761 aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc aaaacgtgct
     8821 ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc caacctgatg
     8881 agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg tagaccggat
     8941 aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt tactgcatca
     9001 tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac tgctttctgt
     9061 ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt gcgcgagaac
     9121 atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat gcttttcgtg
     9181 ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca ctaacggaaa
     9241 tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta aggtgacgca
     9301 aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt tcatagggag
     9361 cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt ctttgtcgat
     9421 taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg acacgcgacc
     9481 attggcaagc gtggtgtaaa gcctggcgcg gcctcgcgtc agataaaaca gccatgacgg
     9541 ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc gcgtatcggc
     9601 tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt gctgcttaag
     9661 gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc atatttagca
     9721 tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt gctgtagaaa
     9781 aaaataacgc aactggaaac agaggaaata aacaatggaa aagagagaaa ttattctgga
     9841 ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac attcggcaat
     9901 agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa cattaattaa
     9961 tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg ggatgccgca
    10021 acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg gactggatgg
    10081 cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga aatatatcat
    10141 cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc cgctttcaaa
    10201 tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg aaattgtgct
    10261 aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca atatctttgc
    10321 cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga tgatgggcct
    10381 cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg aaagggtagg
    10441 cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa cgcagttcga
    10501 atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt atctgatcaa
    10561 ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca gcggcccttg
    10621 ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta ataccaaagt
    10681 cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg tgcgcggcta
    10741 catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat gcaggcccgg
    10801 ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa tcactatgga
    10861 tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt tactgtcagt
    10921 aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac aggtcgtgat
    10981 tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag tcgcttttgg
    11041 cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg gttctctggt
    11101 cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt tcagggtatt
    11161 accggcaatt atcttcgtca ctgcacaggt gagtattctc tactacatcg gtgtgatggg
    11221 gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca gcaagatcga
    11281 gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg cgatcgtcaa
    11341 accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta gtggcatggc
    11401 ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg tggaatattt
    11461 gctggcggca tcgttaatgg cgatccttgg cgggatcttg tttgcccgcc tgttaagccc
    11521 ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa caccgccaaa
    11581 aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg ccgccggtgt
    11641 agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta ttatcggcgg
    11701 cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag gttacctgct
    11761 ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg ccgggagttt
    11821 gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct caccctatct
    11881 gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg cgttgtgcgg
    11941 tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg ttgcgccaca
    12001 ccgtgcgccg gaaatcgccc ggcttggttt acgcgcgctg gcggcggcga cactttctaa
    12061 tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg caatttatga
    12121 gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat tggcactaat
    12181 gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc gcgtgaaatt
    12241 gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc cagaaagcta
    12301 tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt cgtatgcgct
    12361 ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga ctcatcgggc
    12421 aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc ggcattacgg
    12481 tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccgataa agtgggtttc
    12541 agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc tgaaacggta
    12601 tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc ctcggctgtc
    12661 aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga cccgttcagt
    12721 agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac ggtttgatcg
    12781 tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg aacgcagccg
    12841 gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata aaagtcatcg
    12901 ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg ggcgatattg
    12961 cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc ttgcgggtaa
    13021 tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac gcagtattcc
    13081 tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa atccataata
    13141 tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt ctgctgaatc
    13201 agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat cttcaggcta
    13261 ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag atatccgggt
    13321 tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata atccagcgga
    13381 acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaatg cggctgccaa
    13441 aggtactttt cgcatcgtta aggtgcatcc cgcgcagata cttaaagccg acaatacggg
    13501 caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac ccggcagcga
    13561 aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg ccgtcgataa
    13621 tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg gtgttttcaa
    13681 tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg gcaatacgcg
    13741 caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag ttgagcaaag
    13801 aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc gatttttcca
    13861 gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga agaatttgcg
    13921 ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt tgcgtcgtga
    13981 gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg gttgcgtcga
    14041 tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg tgcgctccaa
    14101 tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat gatagcgggt
    14161 taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata gttaatcgca
    14221 ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg tttcgcccca
    14281 gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc cattgccagc
    14341 aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag gtggaacgag
    14401 ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat tttgcttttc
    14461 tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat gaggaacgga
    14521 gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc atccgggctg
    14581 atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc aatgtagata
    14641 ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg gtagaggaaa
    14701 atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt actggcttcc
    14761 gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact gctaccggca
    14821 ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag gaagcaagcc
    14881 agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat accgacatcg
    14941 gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg taataaatat
    15001 tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata gatggtgttg
    15061 cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc acccgcaacc
    15121 gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag ggctaacccc
    15181 ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt cataaccctc
    15241 tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg attatatatt
    15301 tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc agttggaagt
    15361 ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc tggcgttgtc
    15421 gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg ttcaactgtt
    15481 tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct atccgcgagc
    15541 gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag acaacggcgc
    15601 gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag ttatcgcccg
    15661 ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca gccaggacgt
    15721 gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac cgtgccacag
    15781 cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg ccgcgccgac
    15841 ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc cgtggatcct
    15901 gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt cacatttacc
    15961 gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg cggtgcgtca
    16021 tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc aggcaggcac
    16081 attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc gtatacatca
    16141 tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg atcccgcaaa
    16201 tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt cgtgacgcag
    16261 cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa tccctgggcg
    16321 atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat cgtctgctac
    16381 aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa actaaaacta
    16441 cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg attgccattg
    16501 gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct caggcaggtc
    16561 cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc ctgatgacca
    16621 gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact tacggtcaga
    16681 actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac aactgggcgg
    16741 tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg ttcccggata
    16801 caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg aactacatct
    16861 ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc acgacagtta
    16921 ttgtctttat catcgttggc gtgctgatga ttatcggtat cttcaaaggc gcgcagcctg
    16981 cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt gcggcgatga
    17041 tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc ggtattgctg
    17101 caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag gtattctggc
    17161 gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt ccgtataccg
    17221 atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc accctggtgt
    17281 tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt ctgacggcgg
    17341 tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac accctggcgt
    17401 gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg ccgcgtaatg
    17461 cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg tttggcaacc
    17521 agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc gcctggctgg
    17581 ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga cacgacatta
    17641 acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca ttcattctgt
    17701 gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc attgactggg
    17761 gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg ttcggctaca
    17821 agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg cagaacgata
    17881 agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct ttttcaattc
    17941 catttccctg acaaatcatg cggatataaa atttaacatt tggattgata attgttatcg
    18001 tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct gttccggctt
    18061 tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac gcaaggggac
    18121 gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg agatatggaa
    18181 tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt tcttgtgcat
    18241 ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca tcttccgtgg
    18301 aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa gacctgcagc
    18361 gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta caactgacga
    18421 acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc tacaccctga
    18481 ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac aatgatttcg
    18541 atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt ggcccgatgt
    18601 cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc aaaaaaatcg
    18661 gtcagaaatg gtcggatacc gttaccgtcg ataccaccat tcaggaacat cgcgatcgcg
    18721 gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt gtgctgggaa
    18781 tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac tcaacgacca
    18841 ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc aatgtcgaat
    18901 ttgcctggac accgaatcaa aatcacgatt ttactgccgg atacggtttc gaccgtcagg
    18961 atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac tgcgtcagcc
    19021 ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag aaagtcgaga
    19081 acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc gacggcaaat
    19141 acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa tggcgtcacg
    19201 acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg tctgccagcc
    19261 agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg ctgacgaccg
    19321 gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt gcctacatgg
    19381 tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca tttaaagcac
    19441 cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc gcatgtaaga
    19501 ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg gggctttact
    19561 acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt ttccgtaacg
    19621 atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca ccgggctacc
    19681 aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta tttagctact
    19741 acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt ccgttcaacg
    19801 atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc agcaacggcg
    19861 aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg gactggaaac
    19921 cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg cagaaacgcg
    19981 ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc ggcgcggcct
    20041 ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc gacaaggatc
    20101 tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg gcagtggatt
    20161 atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca gtaaatactg
    20221 cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac tgtgatcata
    20281 tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt ctgccaatac
    20341 cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga taagcgtcgg
    20401 gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt ccagcatgca
    20461 tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac gctgcacgga
    20521 ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt cagcgccata
    20581 atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc gctgacatta
    20641 aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat ccggtaatgc
    20701 gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc taaatcgtag
    20761 ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac cagtacaatc
    20821 cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc gtcattgcag
    20881 gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg gggtgggaga
    20941 aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca gcgttgctgc
    21001 cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt gttgtgttgt
    21061 ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc acttccgtat
    21121 ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca ataattatcg
    21181 ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt aaatcagctt
    21241 aataactttg cccccacgca gggcggaggc gtcacacctg caggagaaat cataaatgcc
    21301 atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag gactggaaac
    21361 accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc ttattgctgg
    21421 tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca gtttgatgga
    21481 aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc tggattacgc
    21541 caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa tggtcaccgt
    21601 gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg atggcaaagc
    21661 gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta accgtatcgt
    21721 gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa ttcttattgc
    21781 gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg tgcattactg
    21841 cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct ctcttggtgg
    21901 attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc gccatcacaa
    21961 ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt cgcggcgtcg
    22021 ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag gcggcttatc
    22081 tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg gcatttctcg
    22141 atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt gctggcctgc
    22201 aaatgttgct gccccgtggc agactttgga tccagtcgcg gttaacgctg ttagtcttgt
    22261 tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct tacgggctgg
    22321 tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc agccgcgcct
    22381 ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac ggcggcgttg
    22441 aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg ggcgcacagt
    22501 atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc agcggctggc
    22561 tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg gtggcgattg
    22621 gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg gcgtatcgct
    22681 ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag gcgattggct
    22741 atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg gtgcttgcga
    22801 tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg atttgtacca
    22861 cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg ctggcgtttg
    22921 ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt tacttccgcc
    22981 aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga ccggcaatat
    23041 ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg taaccgtttc
    23101 cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata gccgccagca
    23161 agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt cgtgatgtag
    23221 cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac agcacgctgg
    23281 tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat tatcggccaa
    23341 acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg gtggtgatgg
    23401 acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc gcccagcagc
    23461 atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag cgtcacgcca
    23521 ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa gcattgagtg
    23581 acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc aaccgcgttg
    23641 tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc cgaatggcga
    23701 cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc agccacggca
    23761 ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag caggatatta
    23821 ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt gaggcggcga
    23881 tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat aacgacaata
    23941 tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg ttacatctct
    24001 caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa ttaacgaccg
    24061 tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg cagggggcag
    24121 caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg cgtcgccatt
    24181 ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag gcgatgtaac
    24241 cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta tgattgcacc
    24301 gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag gtaatagcgt
    24361 cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa cgaattttaa
    24421 aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc gagcatttat
    24481 ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag gtgttaaccc
    24541 tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct gatactcgca
    24601 ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc aaggctattg
    24661 agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct cagaatgacc
    24721 agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag gcactggcaa
    24781 tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt gggcaaaacg
    24841 tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc tacgacaaag
    24901 cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat ttgattgcta
    24961 aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt cagttcgtac
    25021 tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac gtgattaaag
    25081 aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca atgtgggaca
    25141 ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc aacaaaatcg
    25201 aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca ctgaaagcac
    25261 acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg ctggcgctgg
    25321 tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag gcgaaagcga
    25381 cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc accaactgga
    25441 aaatcgacaa taaagtggtc cgcgtacctt atgttggcgt agataaagac aacttggctg
    25501 aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt attgtatggc
    25561 caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa tacttgttgg
    25621 aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac gttaatttaa
    25681 aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga aaatcgacat
    25741 tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta ttccagggta
    25801 aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg atggtacacc
    25861 aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg gggcgatatc
    25921 ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa gcgatttttg
    25981 atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc gtttcgcaaa
    26041 tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg attatggatg
    26101 aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt attcgtaaat
    26161 taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa atcttccagt
    26221 tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa ccgctggcag
    26281 gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat cagcgttttc
    26341 ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta acgtcactgc
    26401 gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc ctcggtattg
    26461 ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt attcgcgaga
    26521 aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat gccaacgaag
    26581 ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga atttatgcct
    26641 atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa aataaagttg
    26701 gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat tcgatgcggg
    26761 taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat cagcaaaagg
    26821 tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat gaaccgacgc
    26881 gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa ctggcgaaga
    26941 aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg ataacagacc
    27001 gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa acaacaacgc
    27061 aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt cccatgagtg
    27121 cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac gtcgttcttt
    27181 tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg ttgaacttaa
    27241 gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg gcagggttaa
    27301 ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg gcagtggtgg
    27361 ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa atggcgacga
    27421 tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc ggtttgatca
    27481 acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc ggcacgatga
    27541 tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg ccaatttctg
    27601 gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg agtttccgtc
    27661 tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg ttgtggaaca
    27721 aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg gcaaaaatat
    27781 ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc gtgttctatg
    27841 cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac ctcggcttta
    27901 tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc agcggcggtg
    27961 tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc aactatggcc
    28021 tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg attattatct
    28081 tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat tgcggatatt
    28141 gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct ttcttaaact
    28201 tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc agacaaccca
    28261 cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc atcgcctagt
    28321 gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg tcaagattga
    28381 tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata ttgttattcg
    28441 ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa tgcgataacc
    28501 cgctcgccat atcttctaca tgcgatagcc gtactgcata atgccggtgg tgacctgtaa
    28561 cgttgctgcg ccgagcaata aaaactccgc cgcatcttcc taggtttcaa tgccgccgat
    28621 accgctgatt gggaaatcgc gcagttctgg atgggtgcgc atttgctgaa tgaagcgcag
    28681 ggcgatcggt tttaccgctt taccggaata tccggaaata ctcgatttac cgttaacgat
    28741 cggcataccg attttctgat taagatcgat attggtgatg gatttaacgg tgttaattgc
    28801 cgcaatgcca tctgcgccgc cgcgcttcgc cgccagcgcc acttcgcaca tatcaccgat
    28861 attcggcgtc atcttcgcca gcattggcag cgtggaaccc cgtttcactg cccgacaata
    28921 tttttctacc agctccgggc tttgcccgac atcgctaccc atcgcatgag aagtcatttg
    28981 cggacaggag aagttacact cgatcatatc cgcgccagct gctcccattg ctgctcattt
    29041 tcccccatga tcgaagcgat caatactttg tccgggtaat cttccttcag ccgacgcagg
    29101 gcggtcagat tctcttccaa cggatgttca gcaatctgct ccatattttt gaagccgata
    29161 aaaccggtat cttctttcac cagatgatca aaacgcggcg agacttcgtt ggcgataaaa
    29221 aagccgatcg ttttaaacac cacaccgccc caacctgtgt cgtaggcttt ggcacacatc
    29281 tcatagcagt tgcctaccgg cgaagaagag aggcagaacg ggttgggaaa gttcacgccg
    29341 caaaaagtaa tcgaaagatc tttcgttaac atgagcaagc tccctctaaa tagtgatgaa
    29401 tcgcctcggc ggcttctttc ccggttttca cggcatagac cacggttttg ccaccctcaa
    29461 caatatcgcc agcagcaaag acttgcggat cgcgggtctg ataattttgt gttttgatgg
    29521 tgttacgctg ctgctctaac tccgcaaagg catccagtct ggcatgctga ccgacggcga
    29581 gaataatttt atctgccgcc atcgtcagtt cgcctgataa ccgtacatgc ttaaacgtga
    29641 ctttattgcc ttccacggct actggcgtga atccatcgat gatcgaaacg cccagctccc
    29701 gggcgctggc aaactctttt tcgcttgccg gaaactcatc taactcttca cgtgctacgc
    29761 aagttaccgc ctgacagccg agaactttca gcgtgctggc tacgtccatc gcgacatcac
    29821 cgccgccgat aattaatgcg ctttgtggaa tgctgatatc gccttgcgcc tgtcgtacac
    29881 gttgcaggaa gtcgacggca atctcaacgt cgctatgctc aaacagcggt agcccggaac
    29941 cgcttgataa ccccacggtg accagtaccg cgcggttttc cgctttcagt tgctccaggg
    30001 tgagtgtttt accgatttcg ttattgcact taatggtcac gcccattttt tcaatacggg
    30061 cgatctctgc atccagcact gattgcggta accggaattg cggaataccg ttacgcaacc
    30121 agccaccggg gtgcgcttct ttctcataaa tcgtgacgtc ataaccctgg tttgtcagtg
    30181 tcacactggc ctgcaatccg gcaggacctg cgccaataat cgcgactttg ccgagcgttt
    30241 tagtaccggg ctgataaatt tccatcccgg tttgttgttc aaaatcagta acaaaacgct
    30301 gtaagcggcc gatatcaatg ggagcatcaa caccggcacg ggtacaaccg ctttggcata
    30361 atttctccgt cgggcacact ctggcacaaa cggcaccgag ggcattattt tcgcgaattg
    30421 tctcggcagc gcctttaaaa ttacgaaagt agattgagcg aataaatttc cccggatcgg
    30481 tctgtgccgg gcaagcctga ctacagggag cgtcgtgaca taataaacag cgagaggctt
    30541 ctttaatcgc cagtaacggc gtaaaagccg gagtgagttc atccagataa ttttgttgcg
    30601 gcatgtgatc ttccttaatg ctatgagtaa ttatggcgaa cgctaaataa atattgcgtg
    30661 ctgatattaa ggctatgcgc gtggggaaat ttaagctgtg aaaggattca caaatagctg
    30721 cggttcagaa tgttatatcc gcagcttatt gagtgaagta ataattattc tcaataatta
    30781 acagaaaaga gaaaatatta acgatagaaa ataagataaa caatcatgcc gatagataaa
    30841 attccgaagc caatatatgc caccatataa gcgaagaaga gtccgggatt ttttgctcgc
    30901 cagacataca ccggttccgg tgcattttta acccgtttat caatcgcgcc tgcgcgccag
    30961 cctttccatg cttcgcggaa acaaaatatc gggataagga aaaaaacggc aacgacaaaa
    31021 aactgctgta catccataac tactttcctt gtttcttttg taattcaagt aactgttcgg
    31081 gtgtaacggc gggatacccc tgcgcatctt ccggcacctg gtgcatagcc ggaataggga
    31141 ccagcggccc taaaaaacgc ggttcacgtt taaaaatata aaggtcagcc agcgcaccga
    31201 aacgggcggc aaattcgcga atacgtactg acagcatatc tttcggtgac ggtacggcat
    31261 aacactgagc ctgaatcccc atatgcagcg caataaataa tgctcgctca cagtggaaac
    31321 gttgggtgat aataatgaaa tcattagtat cgaaaacttt gcgtgtacgc acgatggaat
    31381 ccagcgtacg aaagcctgcg taatcgagaa caatatctga tgggtcgaca ccagcagcga
    31441 ttaaatcttt gcgcatggtc atcggctcgt tataactttg caatgcgtta tcgccgctca
    31501 gtaatagata atttacctta ccgctgttat aggcattaat cgctccttga atgcggtagc
    31561 gataatactg attaataacg ccggtacgat aatattttgc tgtgccgagc accacgccga
    31621 cctggcggta ggggagatcc tgcaattcgt cgtagatata aggcgcggtt ttccagctca
    31681 tccagcgatc gaggccgagc acagtcaaca gcagcaagcc gatcaggact aacaggctga
    31741 ggaacacgcg ctttaacatg caggagactc gagctaagga aaatttccat caggctactt
    31801 tacccgctgg ttttgcgcaa gaaacagcaa actgtttgcc gggaatttcg acaccaggag
    31861 agggctggca agccggacaa ggcgttcacg ccgcatccgg caccaggctt aagcgagaag
    31921 cacgcggtcg atactgtgac agccgagagc tttcagcgtt gcggaggtgg catcccactg
    31981 aatcaacggc gcatcggctt tttctgccag aaccgcgcgc tggatatccg ggtaatcata
    32041 acccttgaga ttcaacagaa ttaacgcccc ctgcaacggc ggcagagtcg ggttgaatgc
    32101 ggcgttttca gcgtagctgc cgctgaaaat acgaccgtct ttacattcca gcgcgacacc
    32161 gcttggtgac ttactgtaag gcatgtgcga acggtttgcc gctgcaatcg ccgcctgaga
    32221 aagcgcatca ccagtcagcg catagccgtg atcctgctcg tccatcagca gcgttttaat
    32281 ctccagatct ttcggcccaa aggcatccgg cagatagtca cgcagcgcgt gtgcctcgcg
    32341 gcccggcaaa tgaatacgca gatccagacc gctgttcagt tcattcataa actgacggca
    32401 gtgaccacaa ggcgtatagt taacggtgat ggctgcaagc gctttttcac cactcaacca
    32461 agcgtggctg attgcgcttt gttcggcatg aacggtttgc tgcattgttg caccaataaa
    32521 ttccatattg gcaccaaaat accaggttcc gctcacaccg cgcgcaattg cgccaacatt
    32581 aaaattcgac aatggcgtac gcgcacaggc cgccgccagc ggaagtagtg cgaatgccag
    32641 cgcgtcttcg tccagtcccg ttgcgctctt cagcgatgag acttgctccc cggtcaacaa
    32701 agcggggaag tacttgtctg ccagaatagg ttccagtgca gattgcaagt tatccgcaag
    32761 ttgggcaaaa gcggtttgaa aacgtggatg catggcgttg cctcataatc atgtaatggg
    32821 ataacagttt acgaacccgt tatggcttta tatgtgatct gaatctcatt aattatgcaa
    32881 cacgtgaaac ttacatcaaa aatgcgcgac gtatcgcaaa ttttagccca ttactgccag
    32941 aataatcggg aaaaggaacg gtgcgatcag cgaagtaatt atcccgcata acaccagcgc
    33001 tagcgaacta aatgcacctt cctgataatc cagctcggcg cagcgcgccg taccgagggc
    33061 gtgcgaggca gtccccattg ccagaccgcg cgcagctttg gtacgaatac gcatcgcatt
    33121 aagcaatgta tggccaaata ccgcgccgag gatgccgacg aaaatcacgc aaactgcgct
    33181 gattgccgga ataccgccaa tactgccgcc aaccgccatt gcaataggcg tggtgactga
    33241 tttcggcagg attgacgcgg cgatttccgg tgaagcgccc atcaacaatg ccacggaagt
    33301 ccccgtcacc atcgcaacca cgctgccgat aaaacagatg gtgatgatcg atttccagcg
    33361 cgcgcggatc tggtgtagct gctcatataa aggataggcc agcgcgacga ccgccggttg
    33421 cagcagatcg tttagcactt cgctaccttt aaagtaactg tcgtaagaga tgccggtcag
    33481 cattaaaaaa ggaatgatca ccaccattgc taccagcaac gggttaagca ggggaaactt
    33541 ataccgtgcc gccagtttgc gggcggcaaa aaagacaatc aacgtcagcg gtaatgacca
    33601 ccagatattc gccatcattc ttctgatcct ttctgaccta cgactttacg ttcaccgtgt
    33661 accagttgtg aactccagct caccaccaga aaaaccacca gcgtactgac agcacaggaa
    33721 accactaccg ggccaaactg cgcgcggagc aaatcaaaat attgcatgac gcctacgcca
    33781 atcggcacaa acaatagcgc catatagcga atcagtacgt agcaccccgg attgacccat
    33841 ttcgccggaa gaatttgcaa ggccagcagg acaaacagga tctgcatccc gatgatgctg
    33901 cccggaatgg ttaccggtag cagggaagca atgaaaatgc ctgcatacag gcaggcataa
    33961 atcaggatga aagcgcgtaa atattgccag ataatgttca gtgtcttact cataatcaac
    34021 accccagatg agatggattc atcatacaat taattcgtaa aacgtgctac cgatcacatt
    34081 aagaaatcag cgcttatttt ttcgctaccc ggtattacgc aatgagcagc gaactcaggc
    34141 atacatcaag catagagagg gattgcagtt tgcagcaatc cctctttgca gacctttaat
    34201 ccgctgacca taggcgctaa catagcgcct catttttttg cgggtgatga tatgcgtgtg
    34261 ttactggcac cgatggaggg agtgcttgac tctctggtgc gtgaattgct gaccgaagtt
    34321 aacgactacg atctgtgcat caccgagttt gtccgcgtgg tggatcaact gctgccggta
    34381 aaagtctttc atcgcatttg ccctgagcta caaaacgcca gccggacacc atctggtacg
    34441 ctggtgcgcg tgcagttgtt aggtcagttc ccacaatggc tggcagagaa cgccgcccgt
    34501 gcggtggagt taggtttctg gggcgtggat ctcaattgcg gctgcccgtc gaaaacggtt
    34561 aacggtagcg gcggcggggc gacgttactc aaagatcctg aactcatcta ccagggggca
    34621 aaagcgatgc gtgaagctgt accggcgcat ttgcctgtca gcgtgaaagt gcgtctgggc
    34681 tgggacagcg gtgagaagaa atttgaaatc gccgatgcgg ttcaacaggc tggcgctacg
    34741 gagctggtgg tgcatgggcg gacgaaagag cagggttacc gcgcggagca tattgactgg
    34801 caggcgattg gcgagattcg ccagcggctg aatattccgg tgattgccaa cggtgaaatc
    34861 tgggactggc agagcgcgca acaatgcatg gcgatcagcg gctgcgacgc agtgatgatt
    34921 ggtcgcgggg cgctcaatat tcccaacctg agccgggtgg taaaatataa cgaaccgcga
    34981 atgccgtggc cggaggtggt tgctttgctg caaaaatata cccgtctgga aaagcagggc
    35041 gataccgggt tatatcacgt tgcgcggatt aaacagtggt tgagttattt gcgtaaagaa
    35101 tacgatgaag caacggaatt atttcagcat gttcgggtgt tgaataactc ccctgatatt
    35161 gcaagggcta ttcaggcaat tgatatcgag aaactctaaa aaccatagtg atgtttttga
    35221 caatgtgtta gctctttatc aataggccgc acggttgttt ggtggcgtca aaatcgccga
    35281 ggcgttccct gaaggccggg gcagcccaca tggatgtggg ctgagggcgc gttttacagg
    35341 gatgttacct cgcgcccgac ccggtagccg taagggataa gccgagggca ccgcgcagcg
    35401 gcgattttgt tcgccagagc ccgggggggc agggggcggc ggcgattggt cgccccctgc
    35461 gcgctccttg cgccagtgat tatatgaagc aatgcacatg acaggagcgc aacaggatga
    35521 gagactgaaa tattctcgtt attttcggat ctgatgacat taattaattt taaaatatca
    35581 tcttaaatac gccagtcacc accagtagcc caatcaggaa aataatcaat acagcccaga
    35641 gtataatttt cattggcgtt cctttatttg tgagtgctga ataagtatag gaaaaaacga
    35701 agtcctttgc cgttatccat aaacgattaa gataaatcgg aagatatcat ttacttagtc
    35761 tatatttttc tgggtatgtc gttttattgc tactgatgta ttgccacgcg aaactaaaaa
    35821 atatatcgca acacacggta atctccgcgc accgtttctt acctgattat tcccccatga
    35881 atcgtgattc cttttatcca gccatcgcct gttttccgct gttactgatg ctggccgggt
    35941 gtgcgcctat gcatgaaacc cgccaggcgt taagccagca aacgcccgct gcacaagttg
    36001 acaccgcatt acccacggcg ctgaaaaatg gttggccaga cagccaatgg tggctggagt
    36061 atcacgataa tcagctcact tcattaatta acaatgcact acagaacgca ccggatatgc
    36121 aggtggctga acagcgcatt cagctagctg aagcccaggc gaaggcagtt gccactcagg
    36181 atggtccgca gatcgacttt tcggcggata tggagcggca aaaaatgtcg gcagaagggc
    36241 taatggggcc gtttgctctg aacgatccgg ccgcaggtac gaccggtccg tggtacacca
    36301 acggaacttt tggcttaacg gcgggctggc atctcgatat ctggggaaag aatcgggcgg
    36361 aggttactgc ccgcctgggt acggttaaag cacgggcggc ggaacgcgag caaacccgcc
    36421 aattgctggc tggcagcgta gcccgcctgt actgggagtg gcaaacccag gcggcgttaa
    36481 acacggtctt gcagcaaata gaaaaagagc agaacaccat tatcgcgacc gatcgccagc
    36541 tatatcagaa cgggattact tcttcagttg acggtgtgga aaccgatatt aatgccagca
    36601 aaacccggca gcagctcaac gatgtcgcgg ggaaaatgaa aattattgag gcacggttaa
    36661 atgcactcac aaatcaccag actaagtcat tgaagcttaa accggtcgcg ttgccgaaag
    36721 tggcaagcca gcttcctgat gaactggggt actccttact ggcccggcgg gcagatttgc
    36781 aggcggcgca ctggtacgtt gagtcatcgt taagcactat tgatgcggca aaagcggcat
    36841 tttatcctga catcaacctg atggccttcc tgcaacagga tgcgttgcac ttaagcgatc
    36901 tgttccgtca ttccgcgcag caaatgggcg ttacggcagg cctgacgcta cctattttcg
    36961 atagtggtcg tcttaacgcc aatctcgata tcgcaaaagc cgaaagcaac ttgtctatcg
    37021 ccagctacaa caaagcggtg gttgaagcag tgaatgacgt ggcgcgggca gccagtcagg
    37081 ttcagacact ggcggagaaa aaccagcatc aggcgcaaat tgagcgcgat gccttgcgtg
    37141 tggtaggtct tgcgcaggcc cgctttaacg cgggcatcat tgctggttcc cgcgtcagcg
    37201 aagccagaat cccggcgctg cgtgagcggg ccaatggcct gttattgcaa gggcagtggc
    37261 tggatgcctc cattcgactc accggtgcgc tgggcggggg ttacaaacgc tgatgatatg
    37321 cgccttctat acttaacgtt tattcagcgt taagtggaga actcgatggc ataggtcgcg
    37381 ataattaccg cttccgattc ggggatcggc aaagagtgcg cgttattact ggcgcagcag
    37441 gggtttgata ttggcattac ctggcactca gatgaagaag gggcaaaaga taccgcgcgt
    37501 gaggtggtta cccacggcgt acgtgcggag atcgtgcaac tggatctcgg caatctgcca
    37561 gaaggtgcgc aggcgctgga gacactcatc cagcgatttg ggcgtattga tgtgctggtg
    37621 aataatgcgg gtgcaatgac caaagcgccg tttcttgata tggcttttga tgagtggcgc
    37681 aagattttta ccgttgatgt tgatggtgca tttttatgct cgcaaattgc ggctcgtcag
    37741 atggtgaaat aagggcaggg cggtcgcatc atcaacatta cgtcggtaca tgaacatacg
    37801 ccgctgccgg atgccagcgc ctacacagcc gctaaacatg cgctcggcgg gttaaccaaa
    37861 acgatggcgc tggagttggt caggcataag attttggtga acgcagtcgc gcctggggcg
    37921 atagccacgc caatgaacag catggatgat agcgacgtga agcccgacgc ggagccttcg
    37981 attcctctac ggcgttttgg cgcaacgcat gagattgcca gcctggtggt gtggctttgt
    38041 tcggagggcg caaattacac gacagggcag tcgttgatag tggatggcgg ctttatgttg
    38101 gcgaatccgc agttcaaccc agaatagcgc agtgaggtgc gtggatattt caatgggtta
    38161 ggccagataa gacaccagcg tcgcatcagg catggaacac ccggtgccgg atgtggagtg
    38221 aatgcttatc tgtggcaact gctttacgcc tgattatccg gctttttctt cccgcgccgt
    38281 ttcagccacc agcgcacgcc caccaccaga accacgacca gaatcaacca gacccagtgc
    38341 ttcaaatgct ggtcgagatt gtgcaaccac ggcgcaatca cctgaccacc agcgtaacca
    38401 atagtggtaa aaatcaacgc ccaggcaaat gcgccgagaa tattcagcgg cagaaagatt
    38461 ttcggcggca gctggctggc accaatcagc gtcgggccaa tcacccgaaa gccatacata
    38521 aagcgcgtac caatgacaaa cagatacgga tggcgttgga taagtttctg tgcccgctca
    38581 attttatcct gatgtttcga gaaacggcgt aacagcttgc cgccaaaccg ccgcccgcac
    38641 agatagagca cctggtcgcc aatcatgcca ccaagcgcca cagaaagtac caccagtggg
    38701 aactttaata gcccctgatg tgccgcaacg cctcccagca aagtcacggt ttcaccttcc
    38761 gccaggctac cgataaccag cgcggcataa ccatattgtg agatgagtgt attgagatcc
    38821 ataggccagt ttgtctccct ttggttgcct gtaagcatac accctgtgaa tcattcacgc
    38881 tgaaaaagga catacatcag acggtgcaaa ttacatggat gaacaaaaaa taatcacaag
    38941 ctggcttata cttgaactgt caggttcatg aactcacaac aagggggcgg tatgagccat
    39001 gtctggggac tgttttccca tcccgatcgt gaaatgcagg tgattaatcg cgaaaacgaa
    39061 acgatttctc accattacac ccaccacgtt ttgctgatgg cggcgattcc ggtgatttgc
    39121 gccttcattg gcactacaca gattggctgg aattttggcg atggcactat cctgaagtta
    39181 tcctggttta ccggactggc gctggctgtc ttgttttacg gcgttatgct ggctggggtt
    39241 gcggtgatgg ggcgggtcat ctggtggatg gcgcgcaatt acccgcagcg tccgtctctt
    39301 gcgcactgca tggtcttcgc gggctacgtt gcaactccgc tgtttttaag cggtctggtg
    39361 gcgctttatc cactggtctg gttgtgtgcg ctggtcggca cggtggcgct gttttacacc
    39421 ggctacctgc tttaccttgg tattccgagc ttcctgaata tcaacaaaga ggaaggcctt
    39481 agcttctcca gttcgacgct ggcgattggc gtgctggtac tggaagtgct gctcacccta
    39541 accgttatac tatggggcta cggttatcgt ttgttctaga aagttctttg acgttgcatt
    39601 gctggcgtaa atgtcagcaa tgcagcattt actcacgatt ctcctttgac gatcgcactt
    39661 tttgctcgtt atgatggcgt cgctagcctc agtaaatcct tactccggcg gtgcgcaacc
    39721 cgtgcgcgtg aaccactatc tgaatgctca tcatgccgaa atttcgagtt tctttattta
    39781 gcttggccct gatgctggct gtgccttttg caccgcaggc cgttgcgaaa acggcagccg
    39841 ctaccaccgc ttcacaaccg gaaattgcct ccggtagcgc gatgattgtt gatctgaata
    39901 ccaacaaagt gatctattcg aaccacccgg atctggtgcg tccgattgcg tctatcagca
    39961 aattaatgac cgcgatggtt gtgctggatg cacgactgcc gctggatgaa aaactaaaag
    40021 tggatatcag ccagacgccg gagatgaaag gggtctattc gcgcgtacga ctgaatagcg
    40081 aaatcagccg taaagatatg ctgttgctgg cgctgatgtc ttcagaaaac cgcgcggcgg
    40141 caagccttgc gcaccattat cccggtggtt acaaagcctt tattaaggca atgaatgcga
    40201 aagcgaaatc gctcggaatg aacaacacgc gctttgttga acctaccgga ttgtcggtgc
    40261 ataacgtttc aactgcccgt gacttaacca aactgctcat tgccagcaaa caatatccgt
    40321 tgatcgggca gttaagtact acccgggaag atatggcaac cttctctaac ccgacgtata
    40381 cgctgccgtt ccgcaatact aatcatctgg tgtatcgcga taactggaat attcagttaa
    40441 ccaaaaccgg ctttaccaat gcggcgggcc attgtctggt gatgcgtacg gttatcaata
    40501 ataaaccggt ggcgctggta gtgatggacg cgtttggcaa atatacccat tttgccgatg
    40561 ccagccgcct gcgtacctgg attgaaaccg gtaaagtgat gcctgtgccg gcagcagcgt
    40621 tgagctataa aaaacaaaaa gccgcccaaa tggcggcggc ggggcagacg gcacagaacg
    40681 attaagtgaa ttcggatggc gatactctgc catccgtaat ttttactcca cttcctgcca
    40741 gtttttccgt ttactggttt taccgatccc cggattcatg ctattggtcg gatcattctc
    40801 gcgatagaac ttctgcaacg tctccggtgc tttatacaaa tgaccgacgt tatgctcggc
    40861 agggtactgc gcgccgcgct gctgtagcag ttccagcatc tgttctttta acgcatgcac
    40921 atccacgcct tttttcacta tgtaatcctg atggaagaca tagcacataa aatggccgta
    40981 atagagcttg tgcaccagct ggctgtcgat ctccggcggt aaatgctcat accactcggt
    41041 gtcgttacgc cgcagagcga tatccagtgc cagaatgtct tcgacttcat cggaatgcac
    41101 cgcctgataa cgaattgctg cgcccgcagc ggcgaaacgg tgtaaaaacg ctttgctacc
    41161 ttcctccggc gtacagacaa agaaatcgcc ctcggcctgt ttgaaataat ccaccagcca
    41221 cgatttggct tcaccgacgc catcgcctgc catttttaac agcagatgat gctcgtattt
    41281 atcgcgccag tttttcatgc gcggcggtaa atggctgggg aacaggtgac cgaatttttg
    41341 cattgcacgg tcagtaaaat gcggacggaa gaatttcact ttctccagca tcgcatcggt
    41401 gcgtcccttg agattaaaga agaacggcat cttgtcggtg cctaacttat caatcatcag
    41461 gaaggtgtct ttgccgtatt tttccgcaat atcgtagata tcccggtgca tatattcccc
    41521 ggcaaccggc agattttcga agttagccag aatatgacgg cggatttcgg tcagcacttc
    41581 cggctggttg gtgccgatat aaaacacctg ctgatttttt tccgcttcga aggtatcaag
    41641 acgtaccgca aagacggcca gcttcccggc gcaaccagaa gattcaaata accgatccgg
    41701 atcggcgtta taacgtgcgg gcgtgtcggc ttcaatatca cgaacgcggt ggacatagtc
    41761 ataatcgtgg gcgtgacgac catcgtgacg cacatcgtca tctttgatgc gatcgtcatc
    41821 cagcttgcta aggatctgct ccggcgtttc gcccagatca atccccagat ggttcaccag
    41881 cgtcagtttg ccatcttcat ctatacgcgc gaataacgac atttcggtat acgccgggcc
    41941 acgttgcacc agcgagccgc cggagttgtt acaaataccg ccgatgaccg atgcgcctat
    42001 acacgatgat ccaatcaccg aatgcggttc gcgtcccagc ggtttgaggg ctttttccag
    42061 cgaatagagc gtggtgcccg gataggccag cacctgttcg cccttgccga gaacgtgcag
    42121 cttgtcgaga cgcagggtgc tgatgatcac gatatcgcga tcatagtcgt taccgtttgg
    42181 cgtcgatcct tcggtcaggc ctgtattggc agcctgcatc agaataattt tgtcggcggt
    42241 gacgcaggct ttcagcaccc gccacaattc cagtagtgag ccagggaaaa cgacagccag
    42301 cgcgtcgccc tgaccagaac ggaagccctt gcgatagcgg gccgtttttg cgggatcggt
    42361 gagcaggtgt gaatgaccga ccagacgggc aagttcattc aaaaaggctt tattatcagt
    42421 tgttgtcatg gaagacattt tccactcctt gtggtggcga aaaaatatca caaactagaa
    42481 tagcgccttg tatgacaatt cagcgagtga atattttgaa aattcagaca ataaactgag
    42541 cattcttttt ctctctccat catgcttatg gcaaactacg gtttttgcac cattttcgct
    42601 gccacgtcgg gcaacaaagg aagaaaaatc catatgaaat ggctatgttc agtaggaatc
    42661 gcggtgagtc tggtcctgca gccagcactg gcggatgatt tattcggtaa ccatccatta
    42721 acgcctgaag cgcgggatgc gttcgtcacc gatctgctca agaaaatgac ggttgatgag
    42781 aaaattggtc agctgcgctt aatcagcgta ggcccggata atccgaaaga ggcgatccgc
    42841 gagatgatca aagacggtca ggttggggcg attttcaaca ccgtaacccg tcaggatatc
    42901 cgcgccatgc aggatcaggt gatggaatta agccgcctga aaattcctct tttctttgct
    42961 tacgacgtgc tgcacggtca gcgcacggtg ttcccgatta gcctcggtct ggcctcgtct
    43021 tttaacctcg atgcggtgaa aacggtcggg cgtgtctctg cttatgaagc ggcagatgat
    43081 ggcctgaata tgacctgggc accgatggtc gatgtctcgc gcgatccgcg ctggggacgt
    43141 gcttccgaag gttttggcga agatacgtat ctcacctcaa taatgggtaa aaccatggtg
    43201 gaagcgatgc agggtaaaag cccggcagat cgttactcgg tgatgaccag cgtcaaacac
    43261 tttgccgcat acggcgcggt agaaggcggt aaagagtaca acaccgtcga tatgagtccg
    43321 cagcgcctgt ttaatgatta tatgccgccg tacaaagcgg ggctggacgc aggcagcggc
    43381 gcggtgatgg tggcgctgaa ctcgctgaac ggcacgccag ccacctccga ttcctggctg
    43441 ctgaaagatg ttctgcgcga ccagtggggc tttaaaggca tcaccgtttc cgatcacggt
    43501 gcaatcaaag agctgattaa acatggcacg gcggcagacc cggaagatgc ggtgcgcgtg
    43561 gcgctgaaat ccggaatcaa catgagtatg agcgacgagt actactcgaa gtatctgcct
    43621 gggttgatta aatccggcaa agtgaccatg gcagagctgg acgatgctgc ccgccatgta
    43681 ctgaacgtta aatatgatat ggggttgttt aacgacccat acagccattt ggggccgaaa
    43741 gagtctgacc cggtggatac caatgccgaa agccgcctgc accgtaaaga agcgcgtgaa
    43801 gtggcgcgcg aaagcctggt gttgctgaaa aaccgtctcg aaacgttacc gctgaaaaaa
    43861 tcggccacca ttgcggtggt tgggccactg gcggacagta aacgtgacgt gatgggcagc
    43921 tggtccgcag ccggtgttgc cgatcaatcc gtgaccgtac tgaccgggat taaaaattcc
    43981 gtcggtgaaa acggtaaagt gctgtatgcc aaaggggcga acgttaccag tgacaaaggc
    44041 attatcgatt tcctgaatca gtatgaagaa gcggtcaaag tcgatccgcg ttcgccgcaa
    44101 gagatgattg atgaagcggt gcagacggcg aaacaatctg atgtggtggt ggctgtagtc
    44161 ggtgaagcac aggggatggc gcacgaagcc tccagccgga ccgatatcac tattccgcaa
    44221 ggccaacgtg acttgattgc ggcgctgaaa gccaccggta aaccgctggt gctggtgctg
    44281 atgaacgggc gtccgctggc gctggtgaaa gaagatcagc aggctgatgc gattctggaa
    44341 acctggtttg cggggactga aggcggtaat gcaattgccg atgtattgtt tggcgattac
    44401 aacccgtccg gcaagctgcc aatttccttc ccgcgttctg tcgggcagat cccggtgtac
    44461 tacagccatc tgaacaccgg tcgtccgtat aatgccgaca agccgaacaa atacacttcg
    44521 cgttattttg atgaagctaa cggagcgctt tatccgttcg gctatggtct gagctatacc
    44581 actttcaccg tctctgatgt gaaactttct gcgccgacca tgaagcgtga cggcaaagtg
    44641 acggccagcg tgcaggtgac gaacaccggt aagcgcgagg gggccacggt agtgcagatg
    44701 tacttgcagg atgtgacggc ttccatgagt cgccctgtga aacagctgaa aggctttgag
    44761 aaaatcaccc tgaaaccggg cgaaactcag actgtcagct tcccgatcga tattgaggcg
    44821 ctgaagttct ggaatcaaca gatgaaatat gacgccgagc ctggcaagtt caatgtattt
    44881 atcggcactg attccgcacg cgttaagaaa ggcgagtttg agttgctgta atgctttagt
    44941 aagggcgcga cgtttgcgcc ctttgcctga tgttcatatc ttaacttgct gaattttcgc
    45001 cctttgtagg ccggataagg cgctcgcgcc gcatccggca ggaaacgccg gaccgaatat
    45061 gcctcttaag tatttatctg attctgcaac tgaatccttc cgctcaggct aactctgtca
    45121 ttatcaacta tgcttttctc ttaattcgct gaaaacaaca gtaaaagaat gaggaaagca
    45181 tcatgccact cttaaagctc tgggcaggtt cactggttat gttggcagcc gtgagcctgc
    45241 cgctacaggc ggcttccccc gttaaagtcg gttcaaaaat cgataccgaa ggcgcgctgc
    45301 tcggcaacat catcttgcag gtactcgaaa gccacggcgt accgacggtg aataaagtgc
    45361 aactcggaac gactcctgtg gggcgcgggg cgattacttc cggtgaactg gatatctatc
    45421 cggaatatac cggcaatggc gctttcttct ttaaagatga aaacgatgca gcgtggaaaa
    45481 atgccgggca gggctacgag aaagtcaaaa agctcgatgc ggagcacaac aagttaatct
    45541 ggctgacgcc cgcgcctgca aataacacct ggactatcgc cgtgcgtcag gatgtggcag
    45601 agaaaaacaa actcacttcg cttgccgacc tgagtcgtta tctgcaagag ggcggcacct
    45661 tcaaactggc agcctcggca gagtttatcg aacgcgccga tgcgttaccc acgtttgaaa
    45721 aagcctatgg ctttaagctc ggtcaggatc agttgctgtc actggctggt ggcgacacgg
    45781 cggtgacgat taaagccgct gcccagcaaa cctctggcgt taatgccgca atggcttacg
    45841 gcactgacgg tccggtcgcg gcgctggggc tgcaaacctt aagcgatccg caaggcgtgc
    45901 aacctatcta cgcgcctgca ccagtggtgc gtgaatcggt gttgaaggag tatccgcaaa
    45961 tggcacagtg gctacagcca gtcttcgcca gcctcgatgc aaaaacattg cagcaactga
    46021 atgccagcat tgcagtggaa ggactggatg ccaaaaaagt ggctgccgac tacctgaaac
    46081 aaaaagggtg ggcgaagtaa tttcccgtga cttatctacg tattaatcct gttctggcgc
    46141 tgctgttgtt gctgacggca atcgcagcgg cgctgccgtt tatcagttac gcgcctaatc
    46201 gtttagtctc gggtgaaggg cttcatctct ggcaactgtg gccgcaaacg atctggatgc
    46261 tggtgggcgt tggttgcgcc tggctgacag cctgttttgt ccccgctaaa aaaggcagca
    46321 tttttgcact cattctggcg caattcgtct tcgtattgtt ggtgtgggga gctggaaagg
    46381 cggcgaccca actggcgcaa aatggcagtg cgctggcgcg taccagcctc ggcagaggtt
    46441 tctggctggc tgcggcgctg gcattgctgg cctgtagcga tgccatccgc cgaatctcca
    46501 cgcatccgct gtggcgctgg ttgttgcata tgcagattgc cattattccg ctgtggttgc
    46561 tgtactccgg cacgcttaac gatctctcac taatgaaaga atacgccaac cgtcaggatg
    46621 tgtttgacga cgcgctggca caacatctga cgttgctgtt tggtgcggtg ctgcctgcgt
    46681 tagtgattgg tgtgccgttg ggcatctggt gctacttttc cactgcgcgg cagggggcaa
    46741 ttttttctct gctcaatgtc attcagaccg tgccttcggt ggcgctcttt ggcctgttga
    46801 ttgcgccgct tgccgcgctg gttacggcct ttccgtggct ggggaagctc ggcatagcag
    46861 gaaccggaat gacacccgca ctgattgcgc tggtgctcta tgccttgctg ccgctggtgc
    46921 gaggcgtggt agtcggtttg aaccagatcc cgcgcgatgt gctggagagc gccagagcga
    46981 tggggatgag cggggcgcag cgtttcctgc atgttcagtt accgctggcg ttactggtat
    47041 ttctgcgcag cctgcgggtg gtgatggtgc aaactgtagg tatggcggtg attgcggcgt
    47101 taatcggcgc aggcggtttt ggtgcgctgg ttttccaggg gctgctaagc agcgccattg
    47161 atttagtgtt gctgggggtg atcccggtaa ttgttctggc ggtgcttacc gacgcgctgt
    47221 tcgatttgct tatcgcactg ctgaaggtga aacgtaatga ttgaatttag ccatgtcagc
    47281 aaactgttcg gcgcacaaaa agccgttaac gatctcaatc tcaattttca ggaagggagt
    47341 ttttcggtgc tgattggcac atctggctcc ggcaaatcca ccaccctgaa aatgattaac
    47401 cgcctggtgg agcatgacag cggagtgatc cgctttgccg gagaagaaat tcgctcgctg
    47461 ccagtactgg agttgcgccg ccggatgggc tatgccattc aatctattgg cctgttcccc
    47521 cactggagcg tggcgcaaaa catcgctacc gtgccgcaat tacaaaaatg gtcgcgggcg
    47581 cggattgacg atcgtatcga cgaattaatg gcgctactgg ggctggagtc aaatttgcgt
    47641 gagcgttatc cgcatcagct ttccggtggt cagcagcaac gtgtgggagt ggcgcgtgca
    47701 ctggctgccg atccgcaagt cttactgatg gatgaacctt ttggcgcact ggacccggta
    47761 acgcgcggcg cgttgcaaca agagatgacg cgcattcacc gtttgctggg gcgcactatt
    47821 gtgctggtca ctcatgatat tgatgaggcg ctacggctgg cagaacatct ggtattgatg
    47881 gatcacggtg aagtggtgca gcaggggaat ccgctgacga tgctgactcg tccggcgaat
    47941 gattttgtcc gccagttttt tggacgtagt gaactggggg tgcacctgct ttcgttacgt
    48001 agcgtggcgg attacgtgcg tcgcgaagaa cgggcagaag gtgaggcact ggcagaagag
    48061 atgacgctac gcgatgcgct ctccctgttt gtcgcgcggg gatgcgaggt gctgccggtg
    48121 gtgaacacgc agggccagcc ttgcggcacg ctgcattttc aggatctgct ggtggaggcg
    48181 taagcgtatg aagatgttgc gcgatccgct gttctggctc attgctcttt ttgtggcgct
    48241 gattttctgg ctgccttaca gccagccgct gtttgctgcc ttgttcccac aactgccacg
    48301 acccgtttat cagcaagaaa gttttgcagc tctggcactg gctcatttct ggctggtggg
    48361 aatttcgagt ttgtttgcgg tgatcattgg cacaggtgcc ggaattgctg ttactcgccc
    48421 gtggggcgcg gaatttcgcc cactggtgga aactattgcc gccgttggac agacttttcc
    48481 gcctgtcgca gtgctggcga ttgccgttcc ggtgatcggc tttggtctgc aaccagcgat
    48541 tatcgccttg atcctttacg gtgtgctgcc catcctgcag gtgacacttg ccgggctggg
    48601 agcgattgat cccagcgtga cagaagttgc gaaaggtatg ggaatgagtc gaggtcagcg
    48661 actgcgtaag gtcgagctac cgctggcggc tccggtgatt ctggcgggcg tgcgaacttc
    48721 ggtgattatc aacattggta cggcgacgat cgcctcaacg gtaggggcca gtacgctggg
    48781 tacgcccatc atcatcgggc ttagcggatt taataccgcg tatgtgatcc agggggcgtt
    48841 actggtggca ctggcggcga tcatcgcaga ccgcctgttt gaaaggctgg tgcaggcgct
    48901 tagccagcac gcaaaataaa ggtataacct gcgagcatga cgccaccaat tccgcctaac
    48961 gccataaaca ggaacagggc gatgacccca attttagcta tgcgcataat gcactcctta
    49021 tgttaacgaa aggattgtac agtaaagcgc atttgttaac gaatcattaa ataccgagtg
    49081 ggaaaatatc atggccttgt tcttgccaac tggtgagttg ctgctgttgg gcggaggttc
    49141 gattttcacc gcaccacacc agcaatgtac ggccttcgaa tagttcaggg cgtagttgat
    49201 tgagcgagtg ggcgagcgag gacatcaatg cgccatcctt gttgactggc aatccagccc
    49261 tccagccaca gacgggtggt atcctgaata ttccagccaa ccaccagcgc atctttaccc
    49321 tgttttttac gtgccgaagc cagacaaatg gcgatgtagt tgatcagtac gccgtcgagg
    49381 atcgccagca gcgcctggag agtcggttgt tggcactgaa gccgtcggcg cagaggaata
    49441 aacagatgtg tggtgagtgt ctgggcgggg taatcctgac cgcgctcttt gatccacgtt
    49501 cgcaggct
//
LOCUS       DAJTRB010000020        48246 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTRB010000020 DAJTRB010000000
VERSION     DAJTRB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.020"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            177..470
                     /locus_tag="OO330_001236"
     CDS             177..470
                     /locus_tag="OO330_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5882172.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(511..1701)
                     /gene="nepI"
                     /locus_tag="OO330_001237"
     CDS             complement(511..1701)
                     /gene="nepI"
                     /locus_tag="OO330_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5882173.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1912..2364)
                     /locus_tag="OO330_001238"
     CDS             complement(1912..2364)
                     /locus_tag="OO330_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5882174.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="OO330_001239"
                     /gene_synonym="yicO"
     CDS             complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="OO330_001239"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5882175.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3926..5692
                     /gene="adeD"
                     /locus_tag="OO330_001240"
     CDS             3926..5692
                     /gene="adeD"
                     /locus_tag="OO330_001240"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5882176.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAKEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="OO330_001241"
     CDS             complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="OO330_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5882177.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="OO330_001242"
     CDS             complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="OO330_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5882178.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="OO330_001243"
     CDS             complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="OO330_001243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5882179.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="OO330_001244"
     CDS             complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="OO330_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5882180.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="OO330_001245"
     CDS             complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="OO330_001245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5882181.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="OO330_001246"
     CDS             complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="OO330_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5882182.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="OO330_001247"
     CDS             complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="OO330_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5882183.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13514..13603
                     /gene="tisB"
                     /locus_tag="OO330_001248"
     CDS             13514..13603
                     /gene="tisB"
                     /locus_tag="OO330_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5882184.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13883..15067
                     /gene="emrD"
                     /locus_tag="OO330_001249"
     CDS             13883..15067
                     /gene="emrD"
                     /locus_tag="OO330_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5882185.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15075..15572)
                     /locus_tag="OO330_001250"
     CDS             complement(15075..15572)
                     /locus_tag="OO330_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5882186.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15569..15931)
                     /locus_tag="OO330_001251"
     CDS             complement(15569..15931)
                     /locus_tag="OO330_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5882187.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15921..16268)
                     /locus_tag="OO330_001252"
     CDS             complement(15921..16268)
                     /locus_tag="OO330_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5882188.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16377..16790
                     /locus_tag="OO330_001253"
     CDS             16377..16790
                     /locus_tag="OO330_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882189.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16837..18279)
                     /locus_tag="OO330_001254"
                     /pseudo
     CDS             complement(16837..18279)
                     /locus_tag="OO330_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18314..20029)
                     /locus_tag="OO330_001255"
     CDS             complement(18314..20029)
                     /locus_tag="OO330_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5882190.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20196..21089
                     /locus_tag="OO330_001256"
     CDS             20196..21089
                     /locus_tag="OO330_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5882191.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21086..21900)
                     /locus_tag="OO330_001257"
                     /pseudo
     CDS             complement(21086..21900)
                     /locus_tag="OO330_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21900..23516)
                     /locus_tag="OO330_001258"
     CDS             complement(21900..23516)
                     /locus_tag="OO330_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5882192.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23811..24485
                     /locus_tag="OO330_001259"
     CDS             23811..24485
                     /locus_tag="OO330_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5882193.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24524..26185)
                     /locus_tag="OO330_001260"
     CDS             complement(24524..26185)
                     /locus_tag="OO330_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5882194.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLVLRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26381..26809)
                     /gene="ibpB"
                     /locus_tag="OO330_001261"
     CDS             complement(26381..26809)
                     /gene="ibpB"
                     /locus_tag="OO330_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5882195.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26921..27334)
                     /gene="ibpA"
                     /locus_tag="OO330_001262"
     CDS             complement(26921..27334)
                     /gene="ibpA"
                     /locus_tag="OO330_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5882196.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27328..27400)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27639..27971
                     /locus_tag="OO330_001263"
     CDS             27639..27971
                     /locus_tag="OO330_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5882197.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27973..29223)
                     /locus_tag="OO330_001264"
     CDS             complement(27973..29223)
                     /locus_tag="OO330_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5882198.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVM
                     HQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            29324..30424
                     /gene="cbrA"
                     /locus_tag="OO330_001265"
     CDS             29324..30424
                     /gene="cbrA"
                     /locus_tag="OO330_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5882199.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30385..31722)
                     /locus_tag="OO330_001266"
     CDS             complement(30385..31722)
                     /locus_tag="OO330_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5882200.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31797..32945)
                     /gene="dgoD"
                     /locus_tag="OO330_001267"
                     /pseudo
     CDS             complement(31797..32945)
                     /gene="dgoD"
                     /locus_tag="OO330_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32942..33559)
                     /gene="dgoA"
                     /locus_tag="OO330_001268"
     CDS             complement(32942..33559)
                     /gene="dgoA"
                     /locus_tag="OO330_001268"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5882201.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33543..34421)
                     /gene="dgoK"
                     /locus_tag="OO330_001269"
     CDS             complement(33543..34421)
                     /gene="dgoK"
                     /locus_tag="OO330_001269"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5882202.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34418..35107)
                     /gene="dgoR"
                     /locus_tag="OO330_001270"
     CDS             complement(34418..35107)
                     /gene="dgoR"
                     /locus_tag="OO330_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5882203.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35385..36041
                     /locus_tag="OO330_001271"
     CDS             35385..36041
                     /locus_tag="OO330_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882204.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGCSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36087..36899)
                     /gene="yidA"
                     /locus_tag="OO330_001272"
     CDS             complement(36087..36899)
                     /gene="yidA"
                     /locus_tag="OO330_001272"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5882205.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37013..37411)
                     /locus_tag="OO330_001273"
     CDS             complement(37013..37411)
                     /locus_tag="OO330_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5882206.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37651..40065)
                     /gene="gyrB"
                     /locus_tag="OO330_001274"
     CDS             complement(37651..40065)
                     /gene="gyrB"
                     /locus_tag="OO330_001274"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5882207.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40094..41167)
                     /gene="recF"
                     /locus_tag="OO330_001275"
     CDS             complement(40094..41167)
                     /gene="recF"
                     /locus_tag="OO330_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5882208.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41167..42267)
                     /gene="dnaN"
                     /locus_tag="OO330_001276"
     CDS             complement(41167..42267)
                     /gene="dnaN"
                     /locus_tag="OO330_001276"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5882209.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42272..43675)
                     /gene="dnaA"
                     /locus_tag="OO330_001277"
     CDS             complement(42272..43675)
                     /gene="dnaA"
                     /locus_tag="OO330_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5882210.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43972..44052
                     /locus_tag="OO330_001278"
     CDS             43972..44052
                     /locus_tag="OO330_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882211.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44282..44422
                     /gene="rpmH"
                     /locus_tag="OO330_001279"
     CDS             44282..44422
                     /gene="rpmH"
                     /locus_tag="OO330_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5882212.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44439..44798
                     /gene="rnpA"
                     /locus_tag="OO330_001280"
     CDS             44439..44798
                     /gene="rnpA"
                     /locus_tag="OO330_001280"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5882213.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44762..45019
                     /gene="yidD"
                     /locus_tag="OO330_001281"
     CDS             44762..45019
                     /gene="yidD"
                     /locus_tag="OO330_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5882214.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45022..46668
                     /gene="yidC"
                     /locus_tag="OO330_001282"
     CDS             45022..46668
                     /gene="yidC"
                     /locus_tag="OO330_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5882215.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46774..48138
                     /gene="mnmE"
                     /locus_tag="OO330_001283"
     CDS             46774..48138
                     /gene="mnmE"
                     /locus_tag="OO330_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5882216.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11956 a        13044 c        12205 g        11041 t
ORIGIN      
        1 cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa aagtggaata
       61 taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg atgagagcga
      121 tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt gaatccatga
      181 aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc gtatacgccg
      241 agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta caagatttac
      301 gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag ttaatgttgt
      361 cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa cgtaataatc
      421 agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa gaacagcaac
      481 aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca cctttgcagt
      541 aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg aagtcagacc
      601 aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa gctgaataac
      661 cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg tgatccacgt
      721 tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca cgccggtagc
      781 aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca gtattaacgg
      841 cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa gcgacgtacc
      901 aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca cgccgaatcc
      961 cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc cggcgaaaga
     1021 catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc ggaaagtatt
     1081 ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa tccagaaaat
     1141 acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga taagctcgcc
     1201 taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc cgaagatcac
     1261 cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg acgccgacat
     1321 cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa gcaaactgaa
     1381 tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa acaaaataac
     1441 aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg caaacattgc
     1501 cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg
     1561 ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac aggcgacaca
     1621 aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt
     1681 ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa aagccgacca
     1741 ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg tgttgcttga
     1801 gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg tgaacgtctt
     1861 atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt ttcaatgcaa
     1921 ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa aagcattaat
     1981 atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga cctgagcatc
     2041 ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca gcaggttctg
     2101 ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg cgttttgcag
     2161 gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg actttcccat
     2221 ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc tgagacggcg
     2281 aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca ctaccgccag
     2341 cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca tgatatctgg
     2401 aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga gctgcgacca
     2461 ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg atgcagtacg
     2521 acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc atcaccgtgg
     2581 taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc aggctcgaag
     2641 tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga accatcgcca
     2701 ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca gccgttagcc
     2761 ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta acagacgagg
     2821 taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc ttattcatat
     2881 tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca attaatgttc
     2941 ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg agttcaagtg
     3001 ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc ggaatggaat
     3061 agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc accacaatag
     3121 aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata aaaaacccta
     3181 aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc gtgtctttat
     3241 tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat ccgattccac
     3301 tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac cggatacgaa
     3361 acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc ccggtctgcc
     3421 aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc cccattgccg
     3481 gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg ataccggcaa
     3541 tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg aggatttgcg
     3601 ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca atcaattctg
     3661 tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat
     3721 tactcacgta atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc
     3781 cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc
     3841 acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat aataatcaaa
     3901 ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat ttcatcacat
     3961 tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta
     4021 tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt ccggcccaat
     4081 tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata ctccggcttt
     4141 gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
     4201 tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc cgcgcggcct
     4261 gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag ccggattcgc
     4321 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg
     4381 cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
     4441 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
     4501 aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc tgacgctgga
     4561 cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg cgggtattga
     4621 aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc
     4681 gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac cgttgatcaa
     4741 cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt gggagatcgc
     4801 ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
     4861 gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc tgaatcacct
     4921 cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg cgcgtaaggt
     4981 cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct tacaggcgga
     5041 agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg cccgccagcc
     5101 agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc gggtcattga
     5161 cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg aaaatggttt
     5221 tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac ggctggctcc
     5281 ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg cgacagtcag
     5341 ccatgacagc cataatattg tggtgatcgg tcgcagtgcc aaagagatgg cgctggcggt
     5401 caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc aagtacaaag
     5461 tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt cgctggcgga
     5521 acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt
     5581 tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa ccagtcaggg
     5641 gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat aataaaaaaa
     5701 gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc aactgctgaa
     5761 tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc atcaggcaga
     5821 tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg gtaaggccga
     5881 ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca
     5941 ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc
     6001 caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga
     6061 gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc gtggcgataa
     6121 tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg tcagagagcc
     6181 agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg
     6241 ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac tggtcgatac
     6301 caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc actttgtttt
     6361 tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttgt
     6421 cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca taagattccg
     6481 gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata atcgacggga
     6541 agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac agcgccacac
     6601 ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg aatgtcccgc
     6661 gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg ccggtactct
     6721 ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc gagccgctgc
     6781 ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg aacggcagga
     6841 attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc acgccataag
     6901 tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg taggtcgaaa
     6961 tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc aggtagccga
     7021 taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga
     7081 gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg ggttactcct
     7141 gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca ggcctgagat
     7201 agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt gaaaaggtga
     7261 gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga
     7321 aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg tatcgagcac
     7381 tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc cgacaaaccc
     7441 cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac caattaacat
     7501 ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca tcacgtagct
     7561 ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa
     7621 attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg ctaccagcgc
     7681 accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga ccagatcgac
     7741 gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc ggaccacata
     7801 gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca catatttggt
     7861 gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt ccagcgcgtc
     7921 gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc gtagccgcca
     7981 gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc cggcacgcca
     8041 gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc cgacgttatg
     8101 cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc aggccgttaa
     8161 cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca cccagagcac
     8221 ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc ccgtggcgat
     8281 aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa
     8341 cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat cgctacggct
     8401 gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc gcgtgaagta
     8461 aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt agcgataacg
     8521 ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg
     8581 caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac gcgtgccgtg
     8641 cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca ttccggtgag
     8701 gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt caatcaccag
     8761 catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat cggcatgttt
     8821 cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct ggttttcgct
     8881 taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc cttccagctc
     8941 catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca gctggcgcgg
     9001 acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca gttgttcgat
     9061 gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa caatgcccgc
     9121 ctgagtacga atagcagtga tagtctgacc gatatcgtcg tgcagctcac gcgccacgtc
     9181 gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat tgcgcgccag
     9241 ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc ccagtaacaa
     9301 ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat cgcgccaggt
     9361 ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc cttgccagcc
     9421 atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa agcgcgacag
     9481 ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca agtaccagac
     9541 cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga gcggcagcca
     9601 ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg tcagaccgcc
     9661 agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc agggcagcga
     9721 ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc gccagtcacg
     9781 ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac taccgatcat
     9841 caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca gcagccactc
     9901 cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc ccagacgcag
     9961 accaaacgga aataacagca ccgccatatc agggcgctca accagatgca ggctgatact
    10021 ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg taattaagcg
    10081 ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc tacgtcgtta
    10141 ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt cggtgacaag
    10201 cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc cgccacctgg
    10261 cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc aatatccggc
    10321 gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc atccgggcta
    10381 cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac cagcgcagga
    10441 ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct tagcagctcc
    10501 agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc gcgccccggc
    10561 agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa atcaggttcc
    10621 agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat aagggcaacg
    10681 gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac gataagcgcg
    10741 atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta aacatcgtcg
    10801 gatcggactg attacgctgc actttcacga catcttccag cttagcgatt tggcttatca
    10861 tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg tcgctgtcct
    10921 gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa aggccacaaa
    10981 cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg ttgttatgag
    11041 ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct ggcggcacca
    11101 tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg ccagggcgat
    11161 tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca caggtttcga
    11221 ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg gcggcaaaaa
    11281 cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct tcgttgttca
    11341 tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc tgaatattca
    11401 tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc gccagcgccg
    11461 cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag gtcagccact
    11521 ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga cccacatcgg
    11581 tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg ccgtaatggc
    11641 ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa tccgctacca
    11701 actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc gccagcacgt
    11761 catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc tctgcgcgat
    11821 cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg ccaatcgccc
    11881 ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa atatagttgg
    11941 tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct tttggcagca
    12001 tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc agttcacgca
    12061 cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt ttggcagcgt
    12121 taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca gcggcttttt
    12181 ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc ttaggaatgt
    12241 ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct
    12301 gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg atagagatgc
    12361 cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga acctgaccag
    12421 tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc accaggttag
    12481 tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg cgcgccattc
    12541 cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg atttgcgtgc
    12601 tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc gtcacaatct
    12661 taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag cgcttacgcg
    12721 tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg actttccggg
    12781 aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt
    12841 gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg accactgcgg
    12901 cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt ggggaccttg
    12961 ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga caatggaaaa
    13021 tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg cagataacaa
    13081 aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc aaagcacact
    13141 gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa
    13201 ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg tccaaatatt
    13261 actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt tatgcggggg
    13321 ccgcatcgtt acccggcgaa ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta
    13381 accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt gagataccgc
    13441 gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc
    13501 acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc
    13561 agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca
    13621 ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg gagtcggatg
    13681 cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat tgccgctttt
    13741 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
    13801 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
    13861 ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt tattgatgtt
    13921 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
    13981 tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
    14041 gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg tcggtcgccg
    14101 accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac
    14161 ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
    14221 cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt tgcgccatgc
    14281 taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc taatcggcgg
    14341 tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg ttctttgtgc
    14401 tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg atgcaccgcg
    14461 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt
    14521 gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct ccggcgtgct
    14581 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
    14641 tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct tctcaacgtt
    14701 gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga tccccgactg
    14761 gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct ttttcggtgc
    14821 cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct tcctggcggg
    14881 cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
    14941 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga ccttaatggg
    15001 attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc aggggcagcc
    15061 cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
    15121 atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc ctgatagccc
    15181 gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg atgccagtcg
    15241 cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt ttgtaaggtg
    15301 gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca ctcaaacagg
    15361 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
    15421 tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg tttgaaattg
    15481 tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt atgcctgtcc
    15541 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
    15601 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
    15661 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
    15721 gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc
    15781 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
    15841 agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc cctgggtcgg
    15901 caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac gatcaccgcc
    15961 acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag gtcctctttc
    16021 agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc taacccgccg
    16081 gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc gaaatctggc
    16141 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg gatccacgcc
    16201 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc aaggcgggaa
    16261 attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg ttttttttaa
    16321 gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga ggtcagatgg
    16381 gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc atcgctttaa
    16441 tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca acgcttgaaa
    16501 gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga ataaaaggcg
    16561 aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc ctcgatcaaa
    16621 gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt atgcgtaagg
    16681 aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg tgcagcattt
    16741 tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa gcagcctgaa
    16801 taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt ttttctcctc
    16861 caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg gcgaatagcc
    16921 atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt tacgccacgg
    16981 acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa
    17041 ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca ggttatgcat
    17101 ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt tgagcaccag
    17161 tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc
    17221 aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg caaggatatt
    17281 ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg tcggcagtaa
    17341 atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg aacggatgat
    17401 cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca gcttatgtgc
    17461 gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac gttgctctgg
    17521 cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc aggcaaaata
    17581 gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct gcgcccataa
    17641 gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa gttcgtagta
    17701 aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat gcggctcatc
    17761 atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca gaaaatctac
    17821 cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt tcgcctgtaa
    17881 atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg tcagttcgct
    17941 aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac actcgccagt
    18001 gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt gatagccggc
    18061 atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt tgttggtcca
    18121 cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag gcgtacaaac
    18181 cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac tatcaatatt
    18241 ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct gggtatcggt
    18301 caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg gctgggtcgt
    18361 gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg atgaaataac
    18421 tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg acatagacgc
    18481 caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac ggtttcatgt
    18541 ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca
    18601 ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg aaatggaagt
    18661 cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc atcgccactt
    18721 ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg gtaaccagcg
    18781 gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc ggcgcgttgg
    18841 cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat ttgcgcccga
    18901 cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa atacccatac
    18961 tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc gcaccaaata
    19021 acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc agcgtcgggt
    19081 aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac cctggcagca
    19141 ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc ttttgccctt
    19201 ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa
    19261 aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc
    19321 caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg ccctgcataa
    19381 agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg ccgataacca
    19441 gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg atcaccgcat
    19501 acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca ccgtgagaaa
    19561 tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg gagtagagaa
    19621 caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg
    19681 tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg cgcttcagat
    19741 agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc acttcccagc
    19801 ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt tgttccgtgg
    19861 aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa
    19921 gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag gtgatcaccg
    19981 ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc tcatagccct
    20041 tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg tcgggtcagg
    20101 cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc tgttaagttc
    20161 aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg caaagttcag
    20221 atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat gtgatctcca
    20281 gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac cgggtgatct
    20341 ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg tataccgtac
    20401 aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat agcaactgta
    20461 ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta gtctgggaac
    20521 agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt cagcaaagcg
    20581 ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag ttaataactt
    20641 caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt atttatcagt
    20701 ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa attgaaaaat
    20761 taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat atggctgcca
    20821 cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc ggcaagacgc
    20881 cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta aagcaacaag
    20941 gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt catttcagca
    21001 aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc
    21061 acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct
    21121 ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt ttcgacaacg
    21181 taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt
    21241 ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca
    21301 gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg
    21361 cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt tcgcattcgg
    21421 gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt tgagcatcta
    21481 ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc caatggaacg
    21541 catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac cgtggcgcag
    21601 tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt gcgccatgac
    21661 aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg caatgtccgg
    21721 cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc gcgcgccatc
    21781 gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa
    21841 cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga atttggtcat
    21901 tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat taagctatcg
    21961 agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc atcgccacta
    22021 cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt ttgtgacatg
    22081 tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat attgccgacc
    22141 ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg ctctgcagcg
    22201 gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac ttccgcatct
    22261 tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac cacgaagtac
    22321 agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc gtggttgctg
    22381 aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc catgttgccc
    22441 accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc gtgtaccgca
    22501 aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat tcccaccagc
    22561 atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt ttccggtgcg
    22621 gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga gttaccgtgc
    22681 agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag actgaactct
    22741 tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg accaaagata
    22801 aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg
    22861 taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc
    22921 atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat caccaggaag
    22981 gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta
    23041 tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc gccgataatg
    23101 ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc caccgcgtcc
    23161 tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt gatgaaatag
    23221 ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc ttgcttagca
    23281 aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac cgtccaaccg
    23341 ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt cagtgattcc
    23401 ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc ggcgaaggga
    23461 aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact gagcatttcc
    23521 acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg cagacctgga
    23581 gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc gtgatcgctt
    23641 tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga catctgtgag
    23701 cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat cgattaagca
    23761 gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag atgatctaca
    23821 aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg ctaaacagcc
    23881 ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg actatccgta
    23941 aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc agcggcactt
    24001 acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg gtggaggtgt
    24061 taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc
    24121 ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac ttctcccgtc
    24181 gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg ccggtaaaac
    24241 tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat attgaacaag
    24301 agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc gccgatagac
    24361 tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc acctcgttgt
    24421 catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat gccagcgaat
    24481 actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg atactccaga
    24541 agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac ggatagacag
    24601 tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca aacgccagcg
    24661 ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa ttcattttgg
    24721 ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc agggcaccat
    24781 aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc ccaccagatt
    24841 tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcacg aggttggcac
    24901 ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg atcaacgcca
    24961 taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct ggcacaaaga
    25021 cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc agcatctgaa
    25081 cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca attgcggacg
    25141 gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca ctggcgacca
    25201 gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct ttaaagtgca
    25261 ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc tcgacgcgca
    25321 aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc agttgcgcat
    25381 tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg ataatggtat
    25441 ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata attttgtcgc
    25501 cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc tcaacgcgaa
    25561 tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc tgctgagctt
    25621 ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac aaaatcccgc
    25681 aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc atttccattg
    25741 gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta ttggttaccg
    25801 caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta tgcaggatgg
    25861 cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg cgtaatccgg
    25921 agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta taaacgaaca
    25981 ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc atccccgcct
    26041 gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata cctaatccta
    26101 tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa gccagaatac
    26161 tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt ttaattgcta
    26221 cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat cacgggaaag
    26281 aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa
    26341 aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt aacgcgggac
    26401 gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga attaaatcaa
    26461 tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca gccagcgtaa
    26521 aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc tcttcttttg
    26581 gctgctccgg cgtgcctttt acgctcaggc gcgtaccttc cagttgaatc tctaaatctt
    26641 cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa
    26701 tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg gccagtttgt
    26761 caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata gtcatttctc
    26821 cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc cctgacggcg
    26881 agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt tcgatacggc
    26941 gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa ccatttacca
    27001 ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca aagttgcgtt
    27061 cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga gcacctttca
    27121 ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa ccagccacag
    27181 cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga gggtagccgc
    27241 cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat ccaatagcag
    27301 aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga aatcagcgag
    27361 aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca ggcgcgtcct
    27421 catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat cagcggactt
    27481 ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgc
    27541 gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat cgctataact
    27601 catcaggcca acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag
    27661 cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg atgtcccata
    27721 ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg attggcgacg
    27781 atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt accgctgtaa
    27841 tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg cgctcgcgcg
    27901 ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg gccagaatgc
    27961 ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt ccatccacgc
    28021 cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat tggcgtggtt
    28081 ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc gattatccag
    28141 cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa tatccgtttt
    28201 gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca gttgcacaat
    28261 gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca
    28321 gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg ttaaacgtcg
    28381 ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta acggcgcatc
    28441 acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata gctccggcac
    28501 tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca
    28561 tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg gcgtggtttt
    28621 actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct tttgtacgtt
    28681 gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg gatcgagttg
    28741 atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc taaagacatg
    28801 cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat ccattgcatc
    28861 gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga agtcgtaatg
    28921 ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg acttcggatg
    28981 aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct cgacctcgcc
    29041 tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag aaggacgcac
    29101 gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat tggtattggt
    29161 attggtattg gtattggtat tggtgagcag gtgattacgg ggagcaaagg ttatctgttt
    29221 catggcgcgg tcctgataca aaacggttcc cgccatcata cttcacaagg acttcacttt
    29281 cagacaatag tcttttgcca tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt
    29341 ggcgattatt ggcctcggcc cggcagggtc ggcgttggca cgaaagttag ccggcaaaat
    29401 gcaggtgatc gcgctggata aaaagcacca gtgcggtact gaaggtttca gcaaaccttg
    29461 cggcggtctg ctggcaccgg acgcgcagcg atcttttatt cgcgatggac tgacgcttcc
    29521 tgtcgatgtg atcgccaatc cacagatttt cagcgtcaaa actgtcgacg tcgccgcatc
    29581 gctcacgcgt aactaccagc gaagctatat caatattaat cgccatgctt tcgacttgtg
    29641 gatgaaatcg ctgatccccg ccagcgttga ggtttatcac gatagcctgt gccgaaaaat
    29701 ctggcgtgag gatgataaat ggcatgtcat ttttcgtgca gacggctggg agcaacatat
    29761 taccgcccgc tatctggtcg gtgccgatgg cgcaaactcg atggtgcggc gacatctcta
    29821 cccggatcat caaatccgta aatatgtcgc tatccagcag tggttcgcag agaaacatcc
    29881 ggtgccgttc tactcctgca tctttgataa tgcgataact gactgttact catggagtat
    29941 cagcaaagat ggttatttta tctttggcgg tgcctatcca atgaaagacg gtcagacgcg
    30001 tttcacgacg ctgaaagaga aaatgagcgc ctttcagttc cagtttggca aagcggtgaa
    30061 aagtgagaaa tgcacggtgt tgtttccctc acgctggcag gattttgtct gtggtaagga
    30121 caacgccttt ctgattggtg aagcggcggg atttatcagc gccagctcgc tggaggggat
    30181 tagctatgcg ctggatagcg cagagattct gcgtgcggtg ttcctgaaac agccggagaa
    30241 gagcaacgcc gcttactggc gcgccacaca caaactgcgt ttaaaactct tcggcaagat
    30301 agtaaaaagc cgatgcctga ccgcaccggc tttaagaaag tggattatgc gcagtggtgt
    30361 ggcgcatatt ccacagttga aagattatcc aacgcgcttc acatcaccca ccagcaggat
    30421 gtaagagagc gcgccaatca acgcgacggc ggagatataa accagtgcag gcgcgaaacc
    30481 gtaaccctgc gccaggtacc ccaccaccag cggaacggtg atgccgccca gaccaccggc
    30541 gaagttaaac acgccgccgg ttaaaccaat caggcgcatc ggtgccagag aagagaccag
    30601 cgaccaggta atcgaagcaa aaccgttgcc gaagaatgcc agcgccatca ggcacataat
    30661 catcatcgga tcgttggtgt agttagcgcc cataatgcag gtggagatca gcaagccgca
    30721 gataatcggc gttttacgcg caaagcccag tgaaaagccc ttacgtacca gcagatccgc
    30781 gacccagcca gagagcagga cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa
    30841 gcctgctttc agcgccgtga ttcctttttc ctgggttaaa tagttcggga accaggttaa
    30901 gaaaaaccac agtgcagaag ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg
    30961 atggaacacc agtttccagt ctttggctgt taacggctga cgcgcctctt tcttcaccgg
    31021 cgcatcacca tccaccagac cgccgccatc acgaatgtaa tccagttcag ctttgctgat
    31081 acctttggtc aggcgcggcg gctgataaac cttaaaccaa atcagcgacc agataatgcc
    31141 gataccacca gtgacaatga acacccagtg ccagctcaac atctcctgaa tccagatcag
    31201 cagcggagtc aaaaacgcca gaccgacaaa ctgaccagac gtataaaaac caacggcgga
    31261 agcgcgttca tgttccggga accagctggt caccatccgg ttattggtcg ggaaagcggg
    31321 cgcttcgaaa ataccggtta tcgcacgcag accaattaat gacattaatc ccgtggcaaa
    31381 gccttggaat aaagtcacca ctgaccaacc aaatatcgca ataaaataag tcacgcgaga
    31441 acctacgcga tctaaaaacc aaccgccggg gatctggcat agcgtataaa gccaggcgaa
    31501 ggccgaaaat acatagccca tttccgcttt cgtaatgccg aactcttcct gaatatgggc
    31561 ggaagccacg gccaggttag cgcggtcaac ataacaaatg accaccgtaa taaagatcat
    31621 caccagcgtc agataacgcc gacgccccgg ctttgctgca ttaacgggaa tatccatagc
    31681 gagctttctc cagattttgg gcatagcgaa gccgctcacc atgccctgta atttacagag
    31741 ggttatattt ttgtattgct gttttagtgc ccgatgaggg gcttacgtgg caggaattac
    31801 cactctgcta cgctattatc ttcatgacgc cagagcggat tacgccagtc cggggcattt
    31861 ttactgaact caataacttt agcttcgtcg atttccacac ctaagcccgg tttcgttaac
    31921 ggtttaaaga agccgccaac catgctgaag tcttctttgt ttttcacaaa gtcgagtaac
    31981 tccgcgcctt tgttgtaatg aatgcccata ctttgttact gaagtaccgc gttataggaa
    32041 acaaagtcga tatgcaggca agccgccagt gcaatcggtc cgaacggaca gtgcggcgca
    32101 agggtcacat cataggcttc tgccattcca gcaattttgt agcattcggt aataccgcct
    32161 gcatgggaga gatccggttg cagaatcgaa ataccgcctg cctccagcac gcgtttaaaa
    32221 tcgaaacgtg agaacatgcg ctcacccgcc gccagtggaa tatgcgtttg tgccgccaat
    32281 ttcgggtagt attcggcttg ttccgccagc accggctctt caataaacag cgggcgatac
    32341 ggctccagtt ctttaatcag cactttcgcc attggcgcgc tgacgcgacc gtggaaatca
    32401 agaccaaact caatctgatt gccaaaagct tcacgaattt gtgccacggt gttgactgcc
    32461 gcatctaccg cgcgggagtt atcaattagc cccagttctt cacaaccgtt cagtttgaag
    32521 gtatcgaagc cgatttcgcg cagggtttta atgccgtcga taacatccgc cggacgatcg
    32581 ccgccgaccc aactgtaggc tttaattttg tcgcgaacca ggccgcccat cagttgccag
    32641 actggcgcat tcagcacttt gcctttgata tcccataacg cctggtcgat cccggcgatg
    32701 gcgctcatca ggattggacc gccacgataa aatccggcgc gatacatcac ttgccataag
    32761 tcgttgatgc gcgaaggatc ctgaccaatc aaatagtcac ccagctcgtg aactgccgct
    32821 tccaccgtac gggcgcggcc ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg
    32881 gtttcaattt tcaggaacat ccagcgggga ggtaaacgat acgtggtaat tttggtgatt
    32941 ttcattgcac tgcctctcga tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt
    33001 ctacggactg cccggcgcga taaagatcgc tgcctaagcc cgccccaaca caacctgcgt
    33061 ctatccactg cgccaggttt tctggcgtca cgccgccaac ggcaaagact gcgatgtccg
    33121 gtggtaatac cgcttttaac gctttgatgt attgcggacc aaaagccgat gacggaaata
    33181 ttttcagcgc ctgcgcgccc gcttcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc
    33241 cggggcaaac ggtcatgccg tagcccaccg cgcggcggat cacttcacta tggatattgg
    33301 gcgtaacgat gagctgacag cccatcctgg cgagcgcatc gacctgttca ggtttcagta
    33361 ccgtacctgc gccaatcaac gccttgtcgc catacgcatc aacgatggcg ggaatgctct
    33421 gctcccattg tggggaattc agcgggattt caaccgcgtc gaacccggcg tcaatcaccg
    33481 cgccaacatg cgccagcgcc tcgtcgggcg taatgccgcg caaaatggcg atcagcggga
    33541 gtttagtttg ccactgcatg agcgatgctc cttataccag cctgaaatgc cgtgtcgccc
    33601 gccaccgccg ccacgtcgca acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc
    33661 gatgttccgg cgacaagggt gatggcgtgt tgatgggtca catagtcgcg catactggca
    33721 acctctgcgc caatcaacaa accagagaga aattcgctga cctgttcgcg gggaagtgtt
    33781 cccagcacat gcgaggcgcg aacttcaaaa agctgcggca atatggcggg cgcattaagg
    33841 ccgcgctcaa ggccagccgc gaaggcatcg gcagagtttt cctgcggcgg caaacctgcg
    33901 ccaatcaatg agtgatttaa cagtaaatga tgtaattcac cggtcatcac ggtgcgaaaa
    33961 tcgttgattt gctggctatc ggcctgcacc catttgcaat gcgtaccggg cataacataa
    34021 agagaggaag gagccagagc tcgcgcgccg atcaattgtg tttcttcgcc gcgcatcaca
    34081 ttgtggttat cgtcatgaga gacgcataat ccgggaataa tccagatatt gtcgccaaca
    34141 gacgttaatt gttcgccaat agacgaaaaa cgggcaggaa cagataaata cggagcaact
    34201 ttccagccga cattgctgcc gaccattcct gccatcacca ctggcgtatt ttcttcacgc
    34261 cagtcggtcg tgacttctgc taacaccgca gccggagatt ttccgttcag gcgcgtgacg
    34321 cctgcttctg attgcctgct ctccaggcag tggtcgccct gataaagcca ggcgcgcaga
    34381 ttggtcgatc cccagtcaat tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc
    34441 gtgtcgagct ggcaatcatg gtaagcgccg cctgctctgc cgcatcgccg tcctgatgcc
    34501 gtatcgcatc gaacagcgcc ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc
    34561 ccatccaggt tcgttcaaaa accgcccgct gcagcgaact gatcgcaatg ctaagttgct
    34621 gtaacaccgg gttatgcacc gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt
    34681 taaacgcttc gcggtcctga ttgttggcaa tcatctcgtt cagcgccgat tcaatctgcg
    34741 ccagatcgct ggaggtcgcg cgctctgctg cccaacgggc aatcgccggt tccaccagat
    34801 ttcgcacttc gctcatggca ctgataagcc gtgggtcgta gtcgttttcc agcacccatt
    34861 gcagtacgtc agtgtcgagg taattccact ggttacgcgg tgccacaaac gccccgcgat
    34921 aacgtttcat ttcaatcagc cgcttcgcca tcagcgaacg gaacacctca cggatgatgt
    34981 tgcgcgaggt tgcaaactct tcacagagct ccgcctcagc cggaagtggc gagccaggta
    35041 cgtatttgcc gtgaacgatc tgtttaccca gcgtaatgac aatgcgatcg gttttattga
    35101 gagtcatgga gagtccttgt gctctgtatg ttcttctcta ctttaccccg atcgatgcat
    35161 aacgcggcaa ctttgtagta ccagcgtgat gacgttcgcg tttgccgcgc gcgtaatgta
    35221 gtacaaactt atattgttgt gctacaattt agatcaaaaa aagaacaata cataaaaaaa
    35281 tgacatgcgt cgggcagaaa tctgaaaagg gatatcaggc gctaaacagg agggaaagaa
    35341 gagtatgctt tcaacggctt agctactcgt ttaaaggatt aatcatgaag ttgaatttta
    35401 agggattttt taaggctgcc ggtttattcc cactggcgct gatgctttca ggctgtatct
    35461 cgtatgctct ggtttcccat accgcaaagg gttgttcagg aaagtatcaa tcgcagtcag
    35521 acaccatcac tgggctatcg caggcaaaag atagtaatgg aacaaaaggc tatgtttttg
    35581 taggggaatc gctggattac cttatcactg atggtgccga tgacatcgtt aagatgctca
    35641 atgatccagc acttaaccgg cacaatattc aggttgccga tgacgcaaga tttgttttaa
    35701 atgcggggaa aaagaaattt accggcacaa tatcgcttta ctactactgg aataacgaag
    35761 aagaaaaggc actggcaacg cattatggtt ttgcctgtgg tgttcaacac tgtaccaggt
    35821 cactggaaaa cctaaaaggc acaatccatg agaaaaataa aaacatggat tactcaaagg
    35881 tgatggcgtt ctaccatcca tttaaagtgc gattttatga atactattca cccagaggca
    35941 ttccggatgg tgtttccgca gcattactgc cagtgactgt tacactggac atcattactg
    36001 caccgctgca atttctggtt gtatatgcag taaaccaata atcagtaagc gggcaaacgc
    36061 gtttatgctg tttgcccgcc cacagattaa ttcagcacat acttctcaat agcaaacgcc
    36121 acgccatctt caaggttgga ttttgtgaca aagttcgcca cttctttcac cgaaggaata
    36181 gcgttatcca tcgccacacc gacgcctgca tattcaatca ttgcgatgtc gttttcctga
    36241 tcgccaatcg ccatgatttc ttccggttta atacctaaca cgtcggccag tgatttcaca
    36301 cccgtacctt tgttaacgcg tttatcgagg atttcgagga agtacggcgc acttttcagc
    36361 acggtatatt tctctttcac ttcctgcgga atacgcgcga tagcctggtc gaggatggcg
    36421 ggttcatcaa tcatcatcac tttcaggaac tgggtattgg ggtccatttt ctccgcttcg
    36481 cagaacacca gcggaattgt ggcaacgaag gattcatgca ccgtgtagta gctgatatcg
    36541 cggttagcgg tgtacagcgt ggtgcggtcc agggcgtgga aatgagaacc gacttcgcga
    36601 gagagttttt ccaggaaacg atagtcgtca tagctgagag cggtttgcgc cacggtgcta
    36661 ccatcagcgg ccttctgtac cagcgcgccg ttataagtaa tgcagtagtc gcccggctgt
    36721 tccatatgca gctctttcag gtagttgtgc acacctgcat acggacgacc cgtcgttagc
    36781 acgacattca cgccacgggc gcgagctgcg gcaatcgcat ttttaacggc gggtgaaatg
    36841 gtgtgatcgg gcagcagaag ggtgccatcc atatcgatag caatgagttt gatagccatg
    36901 agttcccaag gtagattagt tcctgaccca tgctaacgcg attccgctca aaaatcagta
    36961 caacacccga gggaaaaggg gatgcaacgc gggttgcatg ctccccattt gcttagcgga
    37021 agagtttccc tttcagcagt tccatgcctg cggaaagcag atcgttattg gcttgtgctg
    37081 acacttcacc ttgcggtgag agcgcatcaa taatcttcgg caattgctct gccagtaaac
    37141 tggaagccgt actggtatcc acgccaagtt tttgcccgag atcggacacc gcatttgtgc
    37201 cgagcgccga ctccagttgc tcgccgctaa ctgattgatt tctctgttga ttactcaacc
    37261 aggttgagag aatggcccct aagccgccac tttgcagttt ttccagcagc acctgaatgc
    37321 caccctgctc ctcaacccaa cttaaaatcg cctgatattt ccccgcatcg cctttcagaa
    37381 aggcaccgac aacttcatca aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg
    37441 acccaaaaag tatagatctg cgggatgata attgcggatt gcagaaataa aaagggcgga
    37501 gatgatctcc gcccttttct tatagcttct tgccggatgc ggcgtgaacg ccttatccgg
    37561 cctacaaaat catgcaaaat caatatattg caggagtctc gtaggactaa taagcgtagc
    37621 gcatcaggca cgctcgcatg gttaacgcca ttaaatatca atattcgccg ctttcagggc
    37681 gttctcttca ataaacgcac gacgcggttc tacggcgtcg cccatcagcg tggtgaacaa
    37741 ctggtcagcg gcaatcgcat ccttaacggt aacgcgcagc atacgacggc tttccgggtc
    37801 catggtggtt tcccacagct gttccgggtt catctcgccc agacctttat aacgctggat
    37861 ggagaggcca cgacgggact ctttcaccag ccagtccagc gcctgctcga agctggctac
    37921 cggctgacga cgctcgccac gttcgataaa cgcatcttct tccagcaagc cacgcagttt
    37981 ctcacccagc gtgcagatac gacgatattc gccaccggtg ataaactcgt gatccagcgg
    38041 atagtcagta tccacaccgt gggtacgcac gcgaacaatc ggctcgaaca ggttttgttc
    38101 ggcattggtg tggacatcaa acttccactg gctgccgtgc tgttctttgt cgttcagttc
    38161 gctgaccagc gcgttcaccc agcgggtaac ggtctgctca tcagaaaggt cagcttccgt
    38221 caacgtcggc tgatagataa gctctttcag cattgctttc ggataacgac gctccatgcg
    38281 gttgatcatt ttctgcgtcg cgttgtactc agataccagt ttctctaacg cttcgccagc
    38341 caatgccggt gcactggcgt tggtgtgcag cgttgcgccg tccagcgcga tagagatctg
    38401 gtactgatcc atcgcttcgt cgtctttaat gtactgttcc tgcttgcctt tcttcacttt
    38461 gtacagcggc ggctgagcga tgtagacgtg accgcgttca acgatttccg gcatctgacg
    38521 atagaagaag gtcaacagca gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat
    38581 gatgatgatg ctgtgataac gcagtttgtc cgggttgtac tcgtcacgac cgataccaca
    38641 gccaagcgcg gtgataagcg tcgccacttc ctgagaagag agcatcttat cgaagcgcgc
    38701 tttctcgacg ttaaggattt tacccttcag cggcagaatc gcctggttct tgcggttacg
    38761 cccctgcttc gcagagccgc ccgcggagtc cccttccacc aggtacagtt cggaaagcgc
    38821 cggatcgcgt tcctggcagt ctgccagttt gcccggcagg ccagctaagt cgagcgcacc
    38881 tttacggcgg gtcatttcac gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat
    38941 aattttgcca accacgattt tcgcatcagt tgggttttcc agcaggtatt ccgccagcag
    39001 ttcgttcatc tgctgttcaa ccgccgattt cacctcagaa gaaaccagtt tgtctttggt
    39061 ctgggaggag aatttcgggt ccggcacttt tacggaaacg accgcaatca ggccttcacg
    39121 cgcatcgtca ccggtggcgc tgactttggc ttttttgctg tagccttctt tgtccatgta
    39181 ggcattcagg gtacgggtca tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg
    39241 ctgcggaatg ttgttggtaa agcagtagat gttttcctgg aagccatcgt tccactgcag
    39301 cgccacttca acgccaatac cgtctttttc ggtggagaag tagaagatat ttgggtggat
    39361 cggcgttttg ttcttgttca gatattcaac gaacgctttg atgccgcctt catagtggaa
    39421 gtggtcttct ttgccgtcac gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa
    39481 cgacaactca cgcagacgtt tcgccagaat ttcatattcg aactcggtca cattggtgaa
    39541 ggtttcgagg ctgggccaga aacgcaccat ggtgccggtt ttttcagtct cgccggtaac
    39601 cgccagcgga gcctgcggta caccgtgttc gtagatctga cggtgaattt taccctcgcg
    39661 ctgaataacc agctccagtt tttgcgacag ggcgtttact accgaaacac caacgccgtg
    39721 cagaccgccg gacactttat aggagttatc gtcaaattta ccgcctgcgt gcagaacggt
    39781 catgatcact tccgccgccg atacgccctc ttccgggtga ataccggtcg gaatgccgcg
    39841 cccgtcatcc tgtacagaga cagagttatc ggcgtgaatg gtgacgataa tttctttaca
    39901 gtgacccgcg agcgcttcgt cgatagcgtt atctaccacc tcgaatacca tgtggtgcag
    39961 accggtgccg tcatccgtgt cgccgatata catacccggg cgcttacgca ccgcatccag
    40021 ccctttcagg actttgatac tggaggagtc ataagaattc gacatcaacg tttctcgctc
    40081 atttatactt gggttaatct gttattttac ccttttccac ggtaaacatc ttcgaatttt
    40141 cgtccgacat gtctataacg tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg
    40201 tcgcttttaa gcggctggca agcaacccgc gacgctcatc atcaagctca gaggcaaaat
    40261 catctatcag gtagagacac cgccgcccgc tttcacgagt gaggaactct ccttgcgcca
    40321 gacgtaaggc gcacatcaac agcttaagct gcccacgcga taaggtatct tccaccggcg
    40381 caccgtcggc gcgaatgcgt aagtccgctt tatgcgggcc atgcgcggtg taggttagct
    40441 ggcgatcgcg ttcaaaatta cgttccagca cctcagcgta ctctgtctct ttctcccagc
    40501 cgcgctggaa agagaaagtc agagaaaact cagggagaaa ttgcttacag gtatcatcca
    40561 tatcggccgc gataccggcg ctatactccg cgcgccaggt gctgatttgc tccgccagcg
    40621 ggatcagttc tttatcccac gggcgtagct gttcgtaacg agtcacctgg cgcagcgccg
    40681 cattgcgctg cttgagcaat cgcttgagat tgctccaggc ggtgaaaaat ccaggttcgt
    40741 tgtgaaagca tccccagtcg aggaatgctc ttctgtattt ggggccgccg ttgagtaaag
    40801 taaacccttc tggcgttatc aactgcatcg gcatcaggtg cgccagttcc gcgactttat
    40861 gcccgtccgt accgtcaata cggactttgc tgtcgccctg tttgtctttg gttaagccaa
    40921 tcgccgtctc gcgctcttcg ccctgtaatc gcccgtggag aacaaatgcc tcctgctcat
    40981 ggcgaatgac gcgaccaatc tgcaaactgc gaaacgcccg accatggccg agcgtataga
    41041 tggcttccag cacgctggtt ttgccactgc cgttggcacc taccagaaag ttaaagccgg
    41101 gagataaagc gagatccgcg gtttcaatat tgcggaaatc gcggatcaac aagcgggtga
    41161 gggacattac agtctcattg gcatgacaac ataggcagcc gactgtgatg cggcatcttc
    41221 aatctgcacg ctggaaaccg aatcggtcag catcatgcgg acgttttcgc atttcagcgc
    41281 gttcagaaca tccagcacat agctgacgtt gaagccgatt tccatctccg caccgctata
    41341 ggtaacgtcg aggatctctt ccgcttcttc ctgttccggg ttgttggcag taattttcag
    41401 ctggttttcg ctgacataca ggcgcacacc gcggaatttc tcgttagaga gaatcgccgc
    41461 gcgagcaaac gcctgcttga gcagatcgca gcctgcttcc agatgtttgt ccgggttctt
    41521 cggcagaacg cgacggtaat ccgggaagcg accatccacc agtttcgagg tgaaaataaa
    41581 gtcgccaacg tgggcacgaa tgttattgct gccaatctgc acacgcagca gattgtcgcc
    41641 gccgtcgagc atacgcatca gttcaatcac gcctttacgc ggtacgatca ccgaatggct
    41701 tggcaaagat tgaccaattg gcattgaaca gaccgccaga cggtggccgt cggttgccac
    41761 ggtgcgcagt tcttcacctt cggtttcaaa cagcataccg tttaagtaat agcgaacatc
    41821 ctgatgcgcc atcgaaaact gggttgcttc aatcagacgc ttcatcgtcg cctgcggcag
    41881 ggtaaattcg acttcgctct gccagtcatc gaggttcggg aaatccgccg ctggcagggt
    41941 agacagcgaa aaacggctac gcccggagcg caccagcatc cgttcacctt ccagctgcac
    42001 ggcaatttcc gcgccttcag gcagaccacg gcagatatca aagaatttgc gcgccggaac
    42061 ggtcgtcgct cctggctcgt gtggctgaac cagcgcaacg cgtgccacca tttccatctc
    42121 gagatcagta ccggtcagcg acaacgtacc gtcagcaacc tgtaacagca gattaccgag
    42181 aatcggtagc gtaggacgac cacctaacgg accgctcacc tgttgtagcg gttttaataa
    42241 atgctcacgt tctacggtaa atttcatagg tttacgatga caatgttctg attaaatttg
    42301 aaaaatcttc tttgatatcg tggctctctt cacgcaactg ctcgatctta cggcaggcat
    42361 gaagcaccgt cgtgtggtca cgcccaccaa acgcatcgcc aatctctggc agactgtggt
    42421 tagtcagctc tttcgctagc gccatcgcca tctggcgcgg acgcgccacc gagcgggatc
    42481 gacgcttgga aaggagatcc gcgactttga tcttgtagta ctccgccacc gtcttctgaa
    42541 tattgtcgat ggtgaccagt ttttcctgca atgccagcaa gtcgcgcagc gcctcacgca
    42601 cgaagtcgat ggtgatcgcc cgtccggtaa agttggcgtt agcaataacg cggttcagcg
    42661 ccccttccag ctcacgtacg ttagatcgta gacgcttggc gataaagaac gccacttcac
    42721 ccggcaaacg aatgtcattt tcgtcggcct ttttcatcag gatcgccaca cgggtttcca
    42781 gctctggcgg ttcgatcgcc acagtcagtc cccaaccgaa gcgggatttc aaacgatcct
    42841 caacgccgtt gatctctttc ggatagcgat ccgaggtgag aatgatctgt tgattacctt
    42901 ccagcagggc gttgaaggtg tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa
    42961 actgaatatc gtcgatcagc agtgcatcta cggaacggta gtagcgttta aactcttcga
    43021 tcgcgttgtt ttgcagggct ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa
    43081 ccactttggc attcggcttg cgcgccataa tgccgttacc caccgcatgc agcaggtgag
    43141 ttttacccag acccgtgccg ccataaagga acaacgggtt ataggcaccg ccagggttat
    43201 ccgccacctg gcaagccgcc gcgcgcgcca gttggttaga tttaccttca acgaagttat
    43261 caaacgtgtg tttgacgttt acgttagaac gataggtcgg ttctgccggg gccgggacgt
    43321 tatcccaacc tgagcgcgta gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg
    43381 caggggccgc gacgttgctc gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc
    43441 cgacttcaaa acgcagctgt ggggcatccg ctccgcagaa actggttagc agtccattga
    43501 tattattaag gtacttgtcc cgtacccaat cgaggacaaa acggtttggc gcgtacaggg
    43561 ccagcgtgtt atcgctcagt tccgcctgca atgggcgtat ccacatactg aattctgtgg
    43621 ctggtaactc atcctgcaat cgggcaagac actgctgcca aagcgaaagt gacacggcgg
    43681 actccactcg aacaaaagtc gataatgact aaggctgaaa cattcatgat tgttgacgta
    43741 cgtcgaaaag accctgcttg agggtgacgc acgaaccgct gtctgcggtt atatgcccga
    43801 tcaagatcct gcaaaacgat cgggaccgcg gatcatagcc taaactgcgc aagagatctt
    43861 ctgtttctca cagattttcc ctatttatcc acaggacttt ccagaactcg ctaagtgtaa
    43921 acgatcctgc cgcgaggcgg gcacgattta cgccgcatat tggaaaattt aatgaccata
    43981 gacaaaaatt ggcttaatcg atctaataaa gatccaggac gatccttgcg ctttacccat
    44041 cagcccgtat aatcctccac ccggcgcgcc atgctggttt ccactggtgt gaggtcgtac
    44101 attttccctg cgaaaaggtg cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg
    44161 tgtgtacaat tattacaatc cggcctcttt aatcacccat ggcttcggtg tccatcgttt
    44221 catttttcgg cggatatcca ataaagccat tgaatttatt caagtttagg tagaaatcgc
    44281 catgaaacgc acttttcaac cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc
    44341 tcgtatggct actaaaaatg gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc
    44401 tcgtctgacc gtttctaagt aataaagcta acccctgagt ggttaagctc gcatttccca
    44461 gggagttacg cttgttaact cccagtcaat tcacattcgt cttccagcag ccacaacggg
    44521 ctggcacgcc gcaaattacc attctcggcc gcctgaattc gctggggcat ccccgtatcg
    44581 gtcttacagt cgccaagaaa aacgttcgac gcgcccatga acgcaatcgg attaaacgcc
    44641 tgacgcgtga aagcttccgt ctgcgccaac atgaactccc ggctatggat ttcgtggtgg
    44701 tggcgaaaaa aggggttgcc gacctcgata accgtgctct ctcggaagcg ttggaaaaat
    44761 tatggcgccg ccactgtcgc ctggctcgcg ggtcctgata gccctcattc gggtctatca
    44821 acgcctgatt agtccgctac tcgggccgca ttgtcgtttc actccaacct gttcaagcta
    44881 cggaattgag gcattgcgca ggtttggagt gataaaaggc agttggttga cggtgaaacg
    44941 cgtattaaaa tgccaccctt tacaccctgg tggtgacgat cccgtcccgc ccggaccatt
    45001 tgataccaga gaacactaac gatggattcg caacgcaatc ttttagtcat cgctttgctg
    45061 ttcgtgtctt tcatgatctg gcaagcctgg gagcaggata aaaacccgca acctcaggcc
    45121 caacagacca cgcagacaac gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg
    45181 gccagtggcc aggggaaact gatctcggtt aagaccgacg tgcttgatct gaccatcaac
    45241 acccgtggtg gtgatgttga gcaagctctg ctgcctgctt acccgaaaga gctgaactct
    45301 acccagccgt tccagctgtt ggaaacttca ccgcagttta tttatcaggc acagagcggt
    45361 ctgaccggtc gtgatggccc ggataacccg gctaacggcc cgcgtccgct gtataacgtt
    45421 gaaaaagacg cttatgtgct ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat
    45481 accgacgcgg caggcaacac gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct
    45541 gtcaacgtca actacaacgt gcagaacgct ggcgagaaac cgctggaaat ctcctcgttt
    45601 ggtcagttga agcaatccat cactctgcca ccgcatctcg ataccggaag cagcaacttc
    45661 gcactgcaca ccttccgtgg cgcggcgtac tccacgcctg acgagaagta tgagaaatac
    45721 aagttcgata ccattgccga taacgaaaac ctgaacatct cttcgaaagg tggttgggtg
    45781 gcgatgctgc aacagtattt cgcgacggcg tggatcccgc ataacgacgg taccaacaac
    45841 ttctataccg ctaatctggg taacggcatc gtcgctatcg gctataaatc tcagccggta
    45901 ctggttcagc ctggtcagac tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc
    45961 caggacaaaa tggcagctgt tgctccgcac ctggatctga ccgttgatta cggttggttg
    46021 tggttcatct ctcagccgct gttcaaactg ctgaaatgga tccatagctt tgtgggtaac
    46081 tggggcttct ccattatcat catcaccttt atcgttcgtg gcatcatgta cccgctgacc
    46141 aaagcgcagt acacctccat ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg
    46201 cgtgagcgtc tgggcgatga caaacagcgt atcagtcagg aaatgatggc gctgtacaaa
    46261 gctgagaagg ttaacccgct gggcggctgc ttcccgctgc tgatccagat gccaatcttc
    46321 ctggcgttgt actacatgct gatgggttcc gttgaactgc gtcaggcacc gtttgcactg
    46381 tggatccacg acctgtcggc acaggacccg tactacatcc tgccgatcct gatgggcgta
    46441 acgatgttct tcattcagaa gatgtcgccg accactgtga ccgacccgat gcagcagaag
    46501 atcatgacct ttatgccggt catcttcacc gtgttcttcc tgtggttccc gtcaggtctg
    46561 gtactgtact atatcgtcag caacctggta accattattc agcagcagct gatttaccgt
    46621 ggtctggaaa aacgtggcct gcatagccgc gagaagaaaa aatcctgatt cggtgagttt
    46681 tcgctaaaat aagggcggtc agttgaccgc cttttttctt ttcgtagggc ggataagcac
    46741 cgcgcatccg ccacacaaag caacaggaac atcatgagcg ataatgacac tatcgtagcc
    46801 caggccacgc ctccgggacg tggcggcgtt ggcatcctgc gcatctccgg cttcaaagcc
    46861 cgtgaagttg ccgaaaccgt gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt
    46921 ccgtttaaag acgccgacgg cagcgtgctc gatcagggga ttgcgctatg gttccctggc
    46981 ccgaactcgt tcaccggcga agatgtgctg gaactgcaag gtcatggcgg tccggtgatc
    47041 ctcgacctgc tgttaaaacg cattctgacc attcccggcc tgcggattgc tcgccctggt
    47101 gagttttccg aacgcgcgtt tcttaacgat aaacttgact tagcccaggc cgaggcgatt
    47161 gccgatctta tcgacgccag ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa
    47221 ggcgcattct ccgcacgggt taatcatctg gtagaagccc tcacccactt gcgcatttac
    47281 gtcgaagcgg caattgattt ccctgatgaa gagatcgatt tcctctccga cggcaaaatt
    47341 gaagcccagc ttaatgacgt gatggccgat ctcgatgcag tgcgtgctga agcacgtcag
    47401 ggtagtttgt tgcgcgaagg gatgaaagta gtgattgccg gacgtcctaa tgccggtaaa
    47461 tcgagcctgt taaacgcgct ggcgggccgt gaagcggcaa tcgtaaccga tatcgccgga
    47521 accacgcgtg acgtgctgcg tgagcatatc cacattgacg gaatgccgct gcatatcatc
    47581 gacactgccg gactacgtga ggccagtgac gaagtggaac gtattggtat cgagcgcgcg
    47641 tggcaggaaa ttgaacaggc cgaccgcgtg ctgtttatgg tcgatggcac cacgacagac
    47701 gccgttgatc cggcagagat ctggccggaa tttatcgccc gtctgcccgc gaaactgccg
    47761 atcaccgtgg tacgcaataa agccgatatc accggcgaaa cgctgggaat gagtgaagtg
    47821 aacggtcacg cgttaattcg tctctcggca aggactggtg aaggcgtgga cgtgctgcgt
    47881 aaccatctca aacagagcat gggctttgac accaatatgg aaggcggctt cctggcgcgc
    47941 cgtcgccact tacaggcgct ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa
    48001 ctgttgggag cctgggcagg tgaactgctg gcggaagagt tgcgcctggc acagcagaac
    48061 ttaagcgaaa tcaccgggga atttacttca gacgacctgc tggggcggat tttctccagc
    48121 ttctgtattg gtaagtaacc gcgcttacga agccgtattc tgactgtcag atgcggcttc
    48181 gcttcattgt tagcactcct gttatgcctc aacccttttt taaacattaa aattcttacg
    48241 taattt
//
LOCUS       DAJTRB010000021        47502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTRB010000021 DAJTRB010000000
VERSION     DAJTRB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.021"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            90..572
                     /gene="sseA"
                     /locus_tag="OO330_001284"
                     /pseudo
     CDS             90..572
                     /gene="sseA"
                     /locus_tag="OO330_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1391..2167)
                     /gene="sseB"
                     /locus_tag="OO330_001285"
     CDS             complement(1391..2167)
                     /gene="sseB"
                     /locus_tag="OO330_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5882217.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAAEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2309..3592)
                     /gene="pepB"
                     /locus_tag="OO330_001286"
     CDS             complement(2309..3592)
                     /gene="pepB"
                     /locus_tag="OO330_001286"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5882218.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3651..3851)
                     /gene="iscX"
                     /locus_tag="OO330_001287"
     CDS             complement(3651..3851)
                     /gene="iscX"
                     /locus_tag="OO330_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5882219.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3863..4198)
                     /gene="fdx"
                     /locus_tag="OO330_001288"
     CDS             complement(3863..4198)
                     /gene="fdx"
                     /locus_tag="OO330_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5882220.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4200..6050)
                     /gene="hscA"
                     /locus_tag="OO330_001289"
     CDS             complement(4200..6050)
                     /gene="hscA"
                     /locus_tag="OO330_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5882221.1"
                     /translation="MALLQISEPGLSAVPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6067..6582)
                     /gene="hscB"
                     /locus_tag="OO330_001290"
     CDS             complement(6067..6582)
                     /gene="hscB"
                     /locus_tag="OO330_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5882222.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6678..7001)
                     /gene="iscA"
                     /locus_tag="OO330_001291"
     CDS             complement(6678..7001)
                     /gene="iscA"
                     /locus_tag="OO330_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5882223.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7018..7404)
                     /gene="iscU"
                     /locus_tag="OO330_001292"
     CDS             complement(7018..7404)
                     /gene="iscU"
                     /locus_tag="OO330_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5882224.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7432..8646)
                     /gene="iscS"
                     /locus_tag="OO330_001293"
     CDS             complement(7432..8646)
                     /gene="iscS"
                     /locus_tag="OO330_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5882225.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8758..9246)
                     /gene="iscR"
                     /locus_tag="OO330_001294"
     CDS             complement(8758..9246)
                     /gene="iscR"
                     /locus_tag="OO330_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5882226.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9516..10256)
                     /gene="trmJ"
                     /locus_tag="OO330_001295"
     CDS             complement(9516..10256)
                     /gene="trmJ"
                     /locus_tag="OO330_001295"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5882227.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10375..11178
                     /gene="suhB"
                     /locus_tag="OO330_001296"
     CDS             10375..11178
                     /gene="suhB"
                     /locus_tag="OO330_001296"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5882228.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11323..12177
                     /locus_tag="OO330_001297"
     CDS             11323..12177
                     /locus_tag="OO330_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5882229.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12368..13648
                     /gene="csiE"
                     /locus_tag="OO330_001298"
     CDS             12368..13648
                     /gene="csiE"
                     /locus_tag="OO330_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5882230.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13640..14779)
                     /locus_tag="OO330_001299"
     CDS             complement(13640..14779)
                     /locus_tag="OO330_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5882231.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14939..15829)
                     /gene="hcaR"
                     /locus_tag="OO330_001300"
     CDS             complement(14939..15829)
                     /gene="hcaR"
                     /locus_tag="OO330_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5882232.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQSSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15965..17326
                     /locus_tag="OO330_001301"
     CDS             15965..17326
                     /locus_tag="OO330_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5882233.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17323..17841
                     /locus_tag="OO330_001302"
     CDS             17323..17841
                     /locus_tag="OO330_001302"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5882234.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17841..18161
                     /locus_tag="OO330_001303"
     CDS             17841..18161
                     /locus_tag="OO330_001303"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5882235.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18158..18970
                     /gene="hcaB"
                     /locus_tag="OO330_001304"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18158..18970
                     /gene="hcaB"
                     /locus_tag="OO330_001304"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5882236.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18980..20192
                     /locus_tag="OO330_001305"
                     /pseudo
     CDS             18980..20192
                     /locus_tag="OO330_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20289..20711
                     /locus_tag="OO330_001306"
     CDS             20289..20711
                     /locus_tag="OO330_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5882237.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20759..21631)
                     /locus_tag="OO330_001307"
     CDS             complement(20759..21631)
                     /locus_tag="OO330_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5882238.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21643..22737)
                     /locus_tag="OO330_001308"
     CDS             complement(21643..22737)
                     /locus_tag="OO330_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5882239.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22770..23768)
                     /locus_tag="OO330_001309"
     CDS             complement(22770..23768)
                     /locus_tag="OO330_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5882240.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23793..25304)
                     /locus_tag="OO330_001310"
     CDS             complement(23793..25304)
                     /locus_tag="OO330_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5882241.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25327..26310)
                     /locus_tag="OO330_001311"
     CDS             complement(25327..26310)
                     /locus_tag="OO330_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5882242.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26407..29508)
                     /locus_tag="OO330_001312"
     CDS             complement(26407..29508)
                     /locus_tag="OO330_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5882243.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29515..29688)
                     /locus_tag="OO330_001313"
     CDS             complement(29515..29688)
                     /locus_tag="OO330_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882244.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29806..30999
                     /locus_tag="OO330_001314"
     CDS             29806..30999
                     /locus_tag="OO330_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5882245.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31197..32450)
                     /gene="glyA"
                     /locus_tag="OO330_001315"
     CDS             complement(31197..32450)
                     /gene="glyA"
                     /locus_tag="OO330_001315"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5882246.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32777..33967
                     /gene="hmpA"
                     /locus_tag="OO330_001316"
     CDS             32777..33967
                     /gene="hmpA"
                     /locus_tag="OO330_001316"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5882247.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGLVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34012..34350)
                     /gene="glnB"
                     /locus_tag="OO330_001317"
     CDS             complement(34012..34350)
                     /gene="glnB"
                     /locus_tag="OO330_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5882248.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34411..35745)
                     /gene="glrR"
                     /locus_tag="OO330_001318"
     CDS             complement(34411..35745)
                     /gene="glrR"
                     /locus_tag="OO330_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5882249.1"
                     /translation="MSHKPAHLLLVDDDLGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35735..36448)
                     /gene="qseG"
                     /locus_tag="OO330_001319"
     CDS             complement(35735..36448)
                     /gene="qseG"
                     /locus_tag="OO330_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5882250.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36613..38040)
                     /gene="qseE"
                     /locus_tag="OO330_001320"
     CDS             complement(36613..38040)
                     /gene="qseE"
                     /locus_tag="OO330_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5882251.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWLAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38616..42503)
                     /gene="purL"
                     /locus_tag="OO330_001321"
                     /gene_synonym="purI"
     CDS             complement(38616..42503)
                     /gene="purL"
                     /locus_tag="OO330_001321"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5882252.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42762..44318
                     /gene="mltF"
                     /locus_tag="OO330_001322"
     CDS             42762..44318
                     /gene="mltF"
                     /locus_tag="OO330_001322"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5882253.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44315..44851)
                     /gene="tadA"
                     /locus_tag="OO330_001323"
     CDS             complement(44315..44851)
                     /gene="tadA"
                     /locus_tag="OO330_001323"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5882254.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44876..45511)
                     /gene="yfhb"
                     /locus_tag="OO330_001324"
     CDS             complement(44876..45511)
                     /gene="yfhb"
                     /locus_tag="OO330_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5882255.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45720..46568
                     /locus_tag="OO330_001325"
     CDS             45720..46568
                     /locus_tag="OO330_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5882256.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46907..47461)
                     /gene="pinE"
                     /locus_tag="OO330_001326"
                     /pseudo
     CDS             complement(46907..47461)
                     /gene="pinE"
                     /locus_tag="OO330_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT        11334 a        13006 c        11946 g        11216 t
ORIGIN      
        1 ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc
       61 cattataagg ggtacgaata aagtaacggg gacttgcagg ctggcagcgc gatgacctgc
      121 tattagaaga aggtgcagta gagctgccgg aaggagagtt taacgccgcg tttaatcctg
      181 aagcagtggt gaaagtaacc gatgtgttat tggcaagcca tgaaaatacg gcgcaaatta
      241 ttgatgcccg cccggctgca cgttttaacg cagaagttga tgaacctcgc ccaggtttac
      301 gtcgcggaca tattcccggt gcattgaacg ttccgtggac ggaactggtg cgcgaaggcg
      361 aactaaaaac gaccgatgaa ctggatgcga tattttttgg tcgcggcgtc agctatgaca
      421 aaccgattat cgtcagctgc ggctctggtg taacggcagc cgtggttttg ttagcactcg
      481 cgacgctgga tgtgccaaac gtgaaactgt acgacggcgc atggagtgaa tggggcgcgc
      541 gggcagattt accggttgag ccagtgaaat aagtatttta caggcaataa aaaaccgccg
      601 aatttggcgt ttttttattg ctggtctggt tcgtggcctt tccagcaggt tgacttgtgt
      661 tacatgagca acgcaggtgc ttcacagcaa aacaatactc accagtaact ctctttttgt
      721 caagcaaaag agagtaatta ttgtttattt agcgtattat cgacaccggc cctttccgcc
      781 gtgttcggta ataaaataac ctggcttatt agtccgaatt cagacaaata taaacaaatc
      841 ctgctcaaaa ttaaaaattc taaccggtaa aagatattac ttaaacatgt aaatttactt
      901 tcctttaaaa aacaaaaaac cgccaaaatc aggcggttat ttgttgctgg tccggttcgc
      961 ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac
     1021 tcgccagtaa ctctcttttt gtcaagcaaa agagagttat tattgttctg ttagtgtatt
     1081 atccactgcg gccctttccg ccgtctcgca agcgggcgct ggcttaagga aaggatgttc
     1141 cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg cggcaatatc gccagtggtg
     1201 ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga tacggtgctt ctgtatcgta
     1261 cttattgttt ctggtgcgct gttttcccaa gtaaataata accggagtct ctccggcgac
     1321 aatttactgg tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttagg
     1381 gcggcatatt ttagattatc cggttttgtt taaagtcacg caaaaaacca ccccagcgac
     1441 gttcatagaa tggcgcaata tgttcggtaa taaagtggct aattcctttt tccccttttt
     1501 tcacctgaca aatatcgatt ggttcatcgc caggtaatgt atcggtcgct acacttcccg
     1561 tcgcctgaat aatttcttcg atatcaccat cggcttcaat gccaataagt aaattaggct
     1621 gtgcctcttc gttctcttta attgaacaaa taaaagcacg cttcaccggc ttaatggttt
     1681 taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg ctctgcgact tccgataata
     1741 tcagcgattc accgccttcc aggatttcct ggctgctcag cggatttccc tcttcaccaa
     1801 tcaacaaact gatttcacgc ggcataaatt ctttaccggt cggcagtttg gcattaagga
     1861 agagcgtttc gccaagggtc atctcaaaca atgtgcgaac gggcattacg acaaatgcct
     1921 gttcgtcttc agccgcctgt tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg
     1981 tgccgtcttc tttttcccag tgctgtaaat caagcgcact atcttcaacc acagcctcgc
     2041 cctgcgccgc cgtaccaggc acccagacgg tggattccag tagagtacgg aaaaaggccg
     2101 ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt
     2161 cggacataag aaccacaatt cattcaacgt tcggggcgca aatgcctgat gcgctacgct
     2221 tatcaggcct acaagggatt cgcaatttgt tgaatttgca gaatttgtag gccggataag
     2281 gcgtttacgc cgcatccggc atattagttt acgccgttaa cagattagct atcgtgcgca
     2341 caccaagtcc cgtagcgccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg
     2401 agcagtcgat atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg
     2461 ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt
     2521 tagacggcag ctggctgcgg tggaattccg ccagcggcag acgccagaat ggttcgttct
     2581 cttgtgaggc actcgccagt aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt
     2641 gataatcatt acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca
     2701 tttccggttt ctgcgcactg gcatcaatca gaccatcggc aagcaccaga cgcccttcgg
     2761 catcagtgtt catcacttca acttttttac cgttgcgata ggtgatgata tcgcccagct
     2821 tgaacgcatt gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt
     2881 tcagtccgcg cataatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt
     2941 ccgacttcat cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag
     3001 tgataccttt acctaccagg cacgcgtaca ccggtgcttc tttatcgcca gttgggttgt
     3061 aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg cccgacagtg tgcagcccca
     3121 tataaccttg ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg
     3181 cgacgttgct gatcagatca acagcacgct gtgccagttg cgatggtccc aattcttctg
     3241 ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct
     3301 ggcgctgggc atcgtccaga tccggccact ccactttacg cgtgcctttc gggcctttat
     3361 aaccttgcca gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat
     3421 gcttgatgcc cagaccgtca atcttgcggg ccgcacgctg gatcagcccc agatcgtctg
     3481 ccccgttcaa atgcagggta atgccgtcat tattaatgct gtaagttgct ttttctcccc
     3541 agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat cgcttctgtc attttagtta
     3601 tccttattcg tttaacagca aaacgggcta cccccaggca gcccgttcat tcactcggct
     3661 tcgtccagcc agactaacaa aatcgcttcg aggattttct cgttggatgc ctgcgggtcg
     3721 tcgtcgaaat cttccagatc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc
     3781 ggatcaagat cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcggtccac
     3841 ttaagtccca tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga
     3901 tttcgaccac taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct
     3961 ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagtgagt
     4021 caaaaccttc acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt
     4081 gttcaatctc aataccgtta cgcagcgctg cgtcgagaat ggtttcaccg ctattagctt
     4141 ccagaacagc gccatcaggg cagaggtcct gatgaggcaa aataacaatc tttggcatat
     4201 taaacctcgt ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga
     4261 gcggcgaaat cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca
     4321 tcatcgccct gcgccacttc actcaggtga gcggcagcat tgtcaatgac ctgacgctct
     4381 gcggcgctta acagcgcggc atcagcagcc agcgcgccgt gcagactttc cagcacacgc
     4441 gccgcttcta ctttttgttc tgccagcata cgggctttta cgtcctgctc ggcatagctc
     4501 attgagtctt tgatcatcga agcgatttcg ctatcggtca gaccgtaaga cggtttgacc
     4561 tgaatagacg cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg
     4621 gcatcgacct ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc cggaatacca
     4681 cgcagcgcaa aacgcgccag tgagcggcag tcctgcacca gttcgcgctc accctgcatt
     4741 acatggatag acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg agcgcgggcc
     4801 accggaatag tggtattacg cggaatcact ttctccacca ggccgcccat cgtttcgagg
     4861 cccagcgaca gtgggatcac atcaagcagc agcatttcgc tgtctggctt gttacccacc
     4921 agaatatccg cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatggaagtc
     4981 agcggtggac gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac gcgagtagaa
     5041 ccgcccacca tcaccacttc cagcacttcg tcagcttcta cacccgcgtc tttcagcgcg
     5101 cgacgacaag ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca
     5161 cggctgattt cgccctgcca gcccgcaacg ttaacggtca cggagtccgc atcgctcagc
     5221 gcgattttgg ctgcaatggt ggcatccagc agttcacgct gaacgcggtt atcgctacga
     5281 tcaggaatgt ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg
     5341 ccgagcgcgg aatcaccgcc ggttgccagc acttcaaaca cgccgcgact taagcgcaga
     5401 atggaaatat caaacgtccc gccaccgagg tcataaacag cgatcacgcc ttcctgaccg
     5461 gaatccagcc cgtaggcgat agccgcagca gtcggttcgt taagtaagcg cagaacatgc
     5521 aaccccgcca gacgcgccgc gtctttggtg ccctgacgct gagcatcgtc aaagtacgcc
     5581 ggaacggtga taactacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc
     5641 agtgctttga ggatgtccgc agaaacgcgc accgggttca gtagccccgc cgccgtttca
     5701 atcatcggca ggccgttttc gctggcctgg aattgataag gcagatgcgg atagcgttgc
     5761 tggatatcag ccagcgagcg ccccatcagg cgtttaacag aactaatggt gttggcggtg
     5821 tcaagcgctg cgttagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga
     5881 acaacagatg gcagcaggtg acggccttca tggtcggcta acgtttcggc ctgaccgctg
     5941 cgcactgtcg ccaccagcga gttggttgtg cccaggtcaa taccagccgc cagacgacgc
     6001 tgatgcggca cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc
     6061 cagaaattaa aaatcgagca gtttttcttc gagttgttcg gcactgctcc gcagtttatc
     6121 gagaaaacgc agcttacgca ccgtatccgc cgccgcgtcc cacgcctcgt tgtctaactg
     6181 ttcaaccatc aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc
     6241 cagccgcgct tcatctttcg cctgttcgat ctcgtccagc tcttcgcgca gctccaactg
     6301 ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg
     6361 taaagaaagc aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt
     6421 aatggttgca gattgctgta cggcggcgag ttgttccgcc aggcttccgc tggcgaattt
     6481 atcaggatga tactgacgtt gtagatcctg aaaacgcagg ctcagtgcct gggtatcgag
     6541 ttgataacgg gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg
     6601 atccgccgcg ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg
     6661 ggttatcggt atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttaacg
     6721 ttcgggttgg tgaatttaaa cccttcattc aggccttctt ttacgaagtc cagctgcgta
     6781 ccgtccagaa attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac
     6841 acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacat
     6901 ccggaggttc tcacgcccag acgcaggcca aaccctttgc cgcggttagc cagaaaggta
     6961 tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta
     7021 ttttgcttca cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag
     7081 aatagaacag tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt
     7141 gatcgcctgc gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga
     7201 agcgatagcg gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc
     7261 gttgacttta atctgcaact tcattacgtc gccacaggcc ggtgcaccca ccatgccgct
     7321 gccgacgttc tcgtcgttgt tatcaaagga acccacgtta cgcggattct cgtaatggtc
     7381 gataactttt tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg
     7441 agcccattcg atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag
     7501 gtcacgcaga cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc
     7561 agtagtaaaa cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc
     7621 cagcgcgcgc agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga
     7681 aactgcgagg tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac
     7741 gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc
     7801 tttgatgccg ttccacagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat
     7861 ctcttctttt gcgatgcgat aggcttcgcc cattccgacg atctggtgaa caggcagagt
     7921 gccggaacgc ataccgcgct cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacacg
     7981 cggtttacga cgcacataca gcgcaccgat acctttcgga ccatagattt tgtgaccgga
     8041 gaaagacatc aggtcaactt tcaactggct caggtcgatt ggcagtttgc ccacgctctg
     8101 ggttgcatca acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat
     8161 atcctgcacc acgccgattt cgttatttac gtgcatgatg gaaacaagga tggtgtcgtc
     8221 acgcatcgcc gcttcgagtt ctttcaggtc gataataccg ttacgctgcg gtgccaggta
     8281 ggtgacttcg aaaccttcgc gctccagctg acggcaagta tccagtaccg ctttgtgttc
     8341 ggttttgctg gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat
     8401 cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc
     8461 gaccagatcg gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa
     8521 acggtgagaa cgagaggccg ggttaccaaa ggttccgtcc atcgtcatga actgcatcat
     8581 tttctcggca acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa
     8641 tttcattgct ctataaactc cgtacatcac tcaatgcaag gaatcaggct accggctgga
     8701 tgtacgaccg tgtttacgga gtatttagca ctccggcctg attctgaatt ctttttatta
     8761 agcgcgtaac ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg
     8821 ctgacgacca gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt
     8881 gttgagaaaa ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt
     8941 atcgccgccc tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac
     9001 ggcgctaatc acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc
     9061 gcctggtcca cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc
     9121 cagataagaa agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc
     9181 ttcagagttg agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag
     9241 tctcatgtct tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg
     9301 gggtattgca tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt
     9361 gactgattta gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct
     9421 gttgtcggat gcggtgtgaa caccttatcc gacctacgat tctgtacatt gtaggcctga
     9481 taagacgcgt taagcgtcgc atcaggcaac cactgttatt cggccttgtt acctttattc
     9541 tgctgctcaa tagaagccag aatcccgcgc aggatattca attcctggct ttccgggcgc
     9601 gcacgggtaa acagacggcg caatttattc atcacctgcc ccggatggtt ttcacggata
     9661 aaaccggttg ccagcagcgt ttgttccaga tgaccataaa aacgctccag atcatcgacc
     9721 agcggatacg gcgtctcttc atgctcgacc tgctcgccat tttcctgagt tgccagccag
     9781 gccatacgca cttcataggc aataacctga accgccattg ccaggttcag cgaactgtat
     9841 tccgggtttg ccgcaatcgc gacatgataa tggcatttct gcaactcttc attggtcaag
     9901 ccgacgcgct cacgaccaaa caccagcgcc accggggtat ttgccgcttc agcgacgctt
     9961 ttcagaccgc attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga gcgtgcgctg
    10021 gtgcccacca ccaggctaca accggctaac gcttcgtcga gcgtatcgac gatgtgagca
    10081 ttaccaatca catcgctggc ccctgcagcc agggcaatcg cctgggagtc gggtttcacc
    10141 agtggattaa ccagccacag attggttaat cccattgttt tcatggcacg ggcaacagaa
    10201 cccatattgc cggtgtgtga cgtctccacc agcacaattc gaatattttg cagcattgtc
    10261 tttcttcatc taaagattat tcacgcatct tatcataaaa cgaagacaga tgccgatctc
    10321 gctgctatac tctgcgccgt tttcccgttc tttaacatcc agtgagagag accgatgcat
    10381 ccgatgctga acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat tgccaaaaac
    10441 tatgaaaccc cggacgctgt agaagcgagc cagaaaggca gtaacgattt cgtgaccaac
    10501 gtagataaag ctgccgaagc ggtgattatc gacacgattc gtaaatctta cccacagcac
    10561 accatcatca ccgaagaaag cggtgaactt gaaggtactg atcaggatgt tcaatgggtt
    10621 atcgatccac tggatggtac taccaacttt atcaaacgtc tgccgcactt cgcggtatct
    10681 atcgccgttc gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc tatgcgtaac
    10741 gaactgttca ccgccactcg cggtcaaggc gcacagctga acggctaccg tctgcgcggt
    10801 agcaccgctc gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt caaagcaaaa
    10861 cagtacgcca ctacctacat caacatcgtc ggcaagctgt tcaacgaatg tgcagacttc
    10921 cgtcgtaccg gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg tgttgacggt
    10981 ttctttgaaa tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct ggttcgtgaa
    11041 gcgggcggca tcgtcagcga cttcaccggt ggtcataact atatgctgac cggtaacatc
    11101 gttgctggta acccgcgtgt tgtgaaagcc atactggcga acatgcgtga cgagttaagc
    11161 gacgctctga agcattaatg actcaggcgg gtgatatcac tcacccgccc tcgcctttca
    11221 ggcgctattc cgaaatactt cctcaccgct ttactttctt tcgtcactct cccaccatct
    11281 ttccccgatt aatggatgat aaagaagtcg caaggaaagg ttatggcact gccagtgaac
    11341 aaacgcgttc ccaaaattct gtttattctc tttgttgttg ccttctgcgt ttatttagtg
    11401 ccgcgcgttg ccatcaactt cttctattat cctgacgata aaatttacgg tcccgacccc
    11461 tggtcggcgg aatccgtcga atttacggct aaggacggta ctcgtctgca aggctggttt
    11521 attccttctt cgacgggccc tgctgacaac gccatcgcaa ccatcattca tgctcacggc
    11581 aatgccggaa atatgtccgc ccactggccg ctggtcagtt ggttacctga gcgtaatttc
    11641 aacgttttta tgtttgatta tcgcgggttt ggtaaatcaa aaggcactcc gtcccaggcc
    11701 ggattgctgg acgatacgca aagcgccatc aatgtggtgc gccatcgcag tgatgtaaac
    11761 ccacaacagc tggtgctgtt cgggcagagt attggcgggg cgaatattct tgctgttatt
    11821 ggtcagggtg atcgtgaagg catacgtgcg gtgatcctcg actccacatt tgcctcttat
    11881 gcaaccatcg ccaaccagat gatccccggc agtggctact tacttgatga gagttacagc
    11941 ggcgaaaatt acatcgccag cgtcagcccg atcccgcttt tactcattca cggtaaagcc
    12001 gatcacgtta tcccatggca gcacagcgaa aagttgtata gcctggcaaa agagccaaaa
    12061 cggttgatcc taatcccgga tggcgaacac attgatgctt tttccgatcg tcacggcaat
    12121 gtttatcgcg aacagatggt ggactttatc cttagcgcgt tgaatccgca gaactaaccc
    12181 atgatcgcta gcacgataat cattcacaaa accaccttaa gacatgctaa tccactggtc
    12241 agaacagttt aaaatgagaa aaattctgtg acgcttgcca acatttctga tgattagcat
    12301 tcccttcgcc atttccctga gcaaacttta gctattctta tcaattatgc ttatgggaga
    12361 tctacagatg atgcctacgc ttgctccacc atctgtcctt tcggctcccc agcgccgctg
    12421 ccagatcttg ctgacgctct ttcagccggg gttgaccgcc accatggcaa ccttcagcga
    12481 gcttaatggt gtggatgatg atattgccag tcttgatatc agcgaaacag gacgggagat
    12541 cctgcgctat catcaactca cactgacgac tggttatgac ggtagctacc gggttgaagg
    12601 tacagtgctt aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt tccgtctgtg
    12661 tccgtcattt attaccagcc aattcacccc cgccctgaag agtgaactga agcggcgcgg
    12721 aattgcgcgt aacttttacg acgataccaa tctacaagcg ttagtgaatc tctgctcccg
    12781 acggctgcaa aaacgctttg aatcgcgcga tattcatttc ctgtgtctgt atctgcaata
    12841 ttgtttgctg cagcaccacg ctggaattac gcctcagttt aatccgctcc aacgtcgctg
    12901 ggcggaatcc tgccttgaat ttcaggtagc gcaggaaatt ggacgccact ggcagcgtcg
    12961 ggcgctccag catgtaccgc ctgatgagcc actgtttatg gcattacttt tttccatgtt
    13021 gcgggttccc gatccactgc gggatgcgca tcagcgggac agacaattgc gtcagtctat
    13081 caaacgtctg gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg atgaacaggg
    13141 gttatgtgat cagctttata tccacctcgc ccaggcgtta aatcgcagtt tgtttgccat
    13201 cggtattgat aataccctgc cggaagagtt cgccagactg tatccacgcc tggtacgcac
    13261 cacccgcgcg gcgctggccg gatttgaaag tgaatacggc gtccatcttt ctgatgagga
    13321 aagtggtctg gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg atctgcatga
    13381 aaaacagatt attctactga ccgggaatga tagcgagcga gaagcgcaga ttgagcagca
    13441 gttacgcgaa ctaacgttac tgccgctcaa cattaagcat atgtcggtaa aggcattttt
    13501 gcagacaggc gctccgcgtg gcgcggcact gattattgcg ccttatacca tgccgttacc
    13561 gctcttttca ccaccgctga tctatacgga cctgacgctg acaacacatc aacaggagca
    13621 gatccgcaaa atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa aacattgccg
    13681 gaagcgccac cagcgccatt acccagaaca cgccgtggcc cagatattga tacaggaaac
    13741 cggcgaaaac ggtcatgata gcgatactgc cgcccatcgc gacggcagag taaaccgcct
    13801 gtaaacggat gacttcgcta acctggcgag cagcaatata acgcatggcg gccaggtggc
    13861 agaccgtgaa ggtgccgcaa tgcagaattt gcaccactat caaccacggc aacgccgtag
    13921 ttgctcccat aatgccccag cgcactacgc cgcaaatcgc cgagatcaac agcatatcgc
    13981 gagcactaca acggcggaaa agtttattac tcagcgcaaa gataatgact tccgccacca
    14041 cgcccagcga ccacaaatac cccaccgccg aggccgagta gccagctgcc tgccagtaaa
    14101 tggcgctaaa accgtaatag gccgcatgtg ccccctgcaa taaacaaacg caggccagaa
    14161 agcgccagtt ctggcgaacc agcgccaacc acgcagacca accggtgctc tcctgctggc
    14221 ggcttgcccc ttgtggctga atcgtcggac ggatgagaaa gccgagcagc atggatgcca
    14281 cgcccaacgt caacagcacg aggatcaccc gataatcaaa catagtgacc agtttgcccg
    14341 tcagcgccga gccaatgaca aacgccaccg agccccacag tcgcactttg ccgtaatcaa
    14401 gcgggaactg cttttgccac gtattcgcca gtgcatcggt caacggtacc agcggtgaga
    14461 aaaagaggtt aaagccaatc atcaccagca tcagccacgc tacgtgcgcc cccgcccaga
    14521 aggcgacagc aaagagaagt ggcagcagtg ccagcacgcg caaggcggaa atcaggcggg
    14581 aaggatcgct gacgcggggc gcgatgagca aactcccgag aaaacgggca accagacctg
    14641 cccccaataa caggccgatg gtttctggcg ttaaaccaat ccctttaagc cagacgctcc
    14701 agaaaggtaa aaaaatgccg taactaaaaa agtatgtgaa atagccgagc gccaaccagc
    14761 gcgtggattg caaaaccatg agtccctccc gtcaggaggc gttagtctgg ggttaatcga
    14821 atgttggtgc aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt ctgtaacgcc
    14881 agttcacaag cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata atgcgtaatt
    14941 atgccgttac gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt aatgccagct
    15001 tcatctcccc tttcttccac gccatcagta aagcaatgga aggcacattt ccggcaatgg
    15061 gacgaaaaac aacctgcccg gtattaaaat tattcatata accgggtatc aaagtgacgc
    15121 ccagccccat gcccaccaga ttcatggtca ccagaatatt cgttgccacc tggacgatat
    15181 ttggctggct gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc gaaccggaat
    15241 acgccggatc ggtactgacg aaattcacgc catccagttg cgcggcggtg atctcttttt
    15301 catgtgctaa agggtgatca accggtaaca ccaccactaa tggttcgtca aaaagctcca
    15361 gataatcaat ctccgggcta taaacaggat ggcgcattaa gccgacgtcg agttcaccac
    15421 ggcgaatttt ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc aaggtgtctg
    15481 gctgtctgag acgaaacatc ggtaatactt ttggcaataa attcacttcc gccgatggca
    15541 caaagccaat ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt aatttggcat
    15601 tttccgcttg ttcgaggatt gccagcgcgt cctggagaaa acattctccc gccgccgtca
    15661 acgcgacttt gcgcttatcc ctcaccagta acggaacacc gacacagttt tcaagatcgc
    15721 ggatctggct gcttaacgaa gactgtgagg tatgcagttt ttccgccgca cgggtaaagt
    15781 tcagtgcctg cgccactgcg acgaaatagc gtaaatgccg tagttccatc accttcccct
    15841 tgttatcgaa aaaacgtctc agctggtaga aattaactat ttcacatatt agcaaccaac
    15901 cagcaacatc cttatggcac aaaaatagaa ggtcaataca tcttatcttt caggattaaa
    15961 aaatatgacc acaccctcag atttgaacat ttaccaactg attgacaccc aaaacggtcg
    16021 ggtcactccg cgtatttata ccgacccgga catttaccaa ctggagcttg agcgtatttt
    16081 cggtcgttgc tggctatttc tcgcccacga aagccagatc ccaaaacccg gtgatttctt
    16141 taacacctac atgggagaag atgcagttgt cgtggtgcgc cagaaagacg gcagcatcaa
    16201 ggcgtttctc aaccaatgcc gccaccgggc catgcgtgtg agttatgcag attgtggcaa
    16261 cactcgcgcc tttacctgcc cgtatcacgg ctggtcttat ggcattaacg gcgagttgat
    16321 cgatgtaccg ctggaacctc gcgcctaccc acaagggttg tgtaaatccc actgggggct
    16381 aaacgaagtt ccttgtgtgg agagttataa aggactgatt tttggcaact gggataccag
    16441 cgcaccgggc ctgcatgatt acctgggtga cattgcctgg tatctggatg gcatgctgga
    16501 tcgtcgcgaa ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga tcaactgtaa
    16561 ctggaaattc ccggcagagc agttcgccag tgaccagtat catgctctgt tcagccatgc
    16621 ttctgccgtt caggtattag gggcgaaaga tgatggcagc gataagcgcc tcggtgatgg
    16681 acaaaccgcc cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg gtcaggacgg
    16741 tcacggtagc ggtttcttct ttactgaaaa accggatgct aatgtctggg tcgatggcgc
    16801 agtttcaagc tattaccgcg aaacctatgc cgaagcagaa caacgtttag gtgaagttcg
    16861 cgccctgcgc ctggcgggtc ataacaatat attccccacg ctttcatggc taaacggcac
    16921 tgccacactc cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt gggcgttctg
    16981 tattactgac aaagccgcct ccgatgaagt taaagccgct tttgaaaaca gcgccactcg
    17041 tgcttttggt cctgctggtt ttctcgagca ggatgactcg gagaactggt gtgaaatcca
    17101 gaaattgctt aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa tggggcttgg
    17161 tcaggaaaag cgccgcgacg acggcattcc tggcattact aactatatct tttcagaaac
    17221 tgccgctcgt ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa gctggcagga
    17281 agtgctcgat aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc gcaagtttca
    17341 ctggagttac atcaccgcat tagccagttt ctctttcacg aagccagtct gctggatgac
    17401 tggaaatttc gtgactggtt ggcacagctc gacgaagaga ttcgttacac catgcgcacc
    17461 acggttaacg cgcaaacacg cgaccgtcgc aaaggcgtcc agccgccgac gacctggatt
    17521 ttcaatgaca ccaaagacca gctggagcgg cgaatcgccc gtctggaaac gggcattgcc
    17581 tgggcagaag agccgccgtc acgcactcgt cacttaatca gcaactgcca ggtaagcgaa
    17641 accgacatcc caaacgtatt tgccgtgagg gtgaattatc tactttatcg ggcacaaaaa
    17701 gagcgcgatg aaacattcta tgttggaacg cgtttcgaca aagttcgccg tctggaagat
    17761 gacaactggc gcttgctgga acgggatatc gtcctggatc aagcggtgat cacttcccat
    17821 aacctgagtg tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg gatgtgccgg
    17881 agggtgaggc tctccggata gatacctcgc ccgtcatcgc cttgttcaac gtgggcggcg
    17941 agttttatgc cattaacgat cgttgcagcc atggtaatgc gtcgatgtca gaagggtatc
    18001 tggaagatga cgccacggtg gagtgcccgc tacacgccgc cagtttttgc ctgaaaacgg
    18061 ggaaagcgtt atgcctgccc gccaccgatc cgctcaccac ttatccagta cacgttgaag
    18121 gtggtgacat tttcatcgac ttaccggagg cgcagccatg agcgatctgc ataacgagtc
    18181 catttttatt accggcggcg gatcgggatt agggctggcg ctggtcgagc gatttatcga
    18241 agaaggcgcg caagttgcca cgctggaact gtcggcggca aaagtcgcca gtctgcgtca
    18301 gcgatttggc gaacatattc tggcggtgga aggtaacgtg acctgttatg ccgattatca
    18361 acgcgcggtc gatcagatcc tgactcgttc cggcaagctg gattgtttta tcggcaatgc
    18421 aggcatctgg gatcacaatg cctcactggt taatactccc gcagagacgc tcgaaaccgg
    18481 cttccacgag ctgtttaacg tcaacgtact cggttacctg ctgggcgcaa aagcctgcgc
    18541 tccggcgtta atcgccagtg aaggcagcat gattttcaca ctgtcaaatg ccgcctggta
    18601 tcctggcggc ggtggcccgc tgtacaccac cagtaaacat gccgcaaccg gacttattcg
    18661 ccaactggct tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc cgtgtggtat
    18721 ggccagcgac ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcga taatgcagtc
    18781 tctgacgccg gagaaaattg ccgccatttt accgctgcaa tttttcccgc aaccggcgga
    18841 ttttacgggg ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat taagcggtgt
    18901 gatgatcaac gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag cggctgggct
    18961 ggatctttaa ggaagcacga tgaaagaaaa aacgatcatt attgtcggtg gcgggcaagc
    19021 ggcggcaatg gctgcggcct cgctacgcca gcaagggttc accggtgagc tgcatctgtt
    19081 ttccgatgag cgacatcttc cttatgaacg acctccactc tcgaaatcca tgttgctgga
    19141 agattccccg cagttacagc aggtgttacc cgctaactgg tggcaggaaa acaatgttca
    19201 tctgcattcc ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt tagtgttaac
    19261 caacggcgaa agctggcact gggatcagct ttttatagca accggcgcgg cagctcgacc
    19321 gctgccgttg cttgatgcac tgggagaacg ctgctttacc ctacaccatg ccggtgatgc
    19381 cgccagactg cgagaagttc tgcagcccga acggtcagtc gtgattgtcg gtgccggaac
    19441 tattggtctg gaactggctg ccagcgccac gcagcgcagc gcagcgcaga tgtaaggtga
    19501 cagtgattga actggcggca accgtcatgg gccgtaatgc accaccgccc gtgcaacgct
    19561 atcttttaca gcgccatcag caggctggtg tgcgcattct gctcaataat gccattgaac
    19621 atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag tggggaaacg cttcaggctg
    19681 atgtggtgat ttacggtatt ggtatcagcg ccaacgagca actggctcgc gaggccaacc
    19741 ttgatactgc caatggcatt gtcattgatg aggcttgccg cacctgcgat cccgcgatct
    19801 ttgccggtgg cgatgtggca atcactcgtc ttgataatgg tgcactacac cgctgcgaaa
    19861 gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc cgcaatgtta gggctaccgc
    19921 taccgctact gccgccgccg tggttctgga gcgatcagta cagtgataac ttacagttta
    19981 ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa cccggaaact cagaaggcga
    20041 tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt cacgctgaat cagggccgtg
    20101 agattcgccc aattcgcaaa tggatccaga gcggcaaaac gtttgatgcg aaactgctga
    20161 tagatgagaa catcgcgctt aaatcactgt aatcaggata attagcgaat atctcaatgc
    20221 ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa atcacattat cttgcaaagg
    20281 gattggttat gaacacacta cgttattttg attttggagc tgcccgcccc gttttgttat
    20341 taattgcccg tatcgccgtg gtcttaattt tcattatttt tggttttccc aaaatgatgg
    20401 gctttgacgg tacggtccaa tatatggcct cgttgggcgc gccaatgccg atgctggcag
    20461 cgattattgc ggtagttatg gaagtgcccg ccgcgatatt aatcgtgctt ggctttttca
    20521 cccgtccgct ggcggtgctg tttattttct acacgctggg tacggcggtg attggtcacc
    20581 attactggga tatgaccggc gatgcggttg ggccaaatat gattaatttc tggaagaatg
    20641 tcagtatcgc tggcgcgttc ttgctattgg caattaccgg accgggggca atttctctcg
    20701 atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct tatccggcat tttataaatt
    20761 acagccactc tacccgcaac gacatctctg aggttgttga ttcccctggt gctagcgcaa
    20821 tgagatcacc cccttccggg cgatgatgat catccggcgc atggctcatc ggttccagac
    20881 agaagaaatc aaacgcatat cctttatcaa acgcagggtc ggaaacaaag atgaaataac
    20941 acggtgcagg tggcgtcgtt tccatgatga tggcataacc ttcctgcggc tgctcgatgc
    21001 gggcctgacc attccatccg gcaaaaccat tgttcaccca ctgacgcggc aacggcgcga
    21061 gctggttaaa atccagttcc tgcggtagct gctcgcaaaa ctcacccgcc agccactgct
    21121 cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg cgtttgtggt gacaacggaa
    21181 aataaggatg ccagccggta ccaaatggca gcgtctctgc cccttgattg gtgacagaga
    21241 gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg acttacccga tagtgataga
    21301 caccgctgcg atgttcatac accagacaca aactatcatc gctgtgcgag acacattgcc
    21361 actcgcccag ccagccatcg ccgtgtagat agtgcgcatc ccactcaaca ttcggttgca
    21421 gttgatactc acgcccctgc cagacaaaac ggttgccgct cacccggttt gcaaatggca
    21481 ccagcggaaa acatgaggca tcggttgcca caccgctttt tttaccagga cgtaagagcg
    21541 gcgtcgtatc gcgccagaag ccttcgatta cgccgccctg gtcagaaacg tccagcttta
    21601 gcgacccgtg ggataaggta tagatggtca tccgctaact ccttaatccg ggaagttaat
    21661 cacaactttc ccgcatttgc cgctcgccat cagcacatag gcatcacctg cctgttctag
    21721 cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc cagtcagtca aatcgtgggc
    21781 gcatttttcc atatggaaca gactggtcac ccaggagccg ataatccgcc gttgatggtg
    21841 catcagatca gcgctgacct cgaattccac ttttccggtt tcaccaatgt aaaccacccg
    21901 cccccagtca gcagtggatt gcagtgccag caagcgacct gcggcattac cggaacaatc
    21961 gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt atctgcggca gaccttcggt
    22021 ggttgctaaa tagccgtgat ccatcccccc taactgtttt gccatcgcca gacgttccgg
    22081 cagcatatca acgccgatga tccgttttgc accgcgacct ttcgccagca tcatcgccat
    22141 catgccgacc ggccccagac cgaccaccag cacgttatcg ctgccggaaa cttcgccgcg
    22201 caaaatccct tcataggccg tgccaacgcc gcaactgata aacgcaccat cttcgtagct
    22261 cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc agcagatatt cggcatgacc
    22321 gccgtcacgc tgccagccgt aagccgcttt tcctttgcca gtacaagaga taggaaagcc
    22381 gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga tacaccagca cgcggtcgcc
    22441 ctctttaaaa tggcggcagc cttgccccat cgccacaatc tgcccgcacg gttcatgacc
    22501 gttgataaat ccctggtata acggtttatc gggtgccgcc gccgtggcac ggtgttgatg
    22561 atagatatag tggacatcgc ttccgcaaat cccggaggat ttcattttga tcagtacctg
    22621 gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg acggttgaat ttcctagtaa
    22681 ataagcagcc agcatcgttt tcataaatcc ctcattaaca atacgattaa ttttcatccc
    22741 tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg ctttactgct tcgctgggtc
    22801 agcaagatat tcgccagcac cgccaccacg atgatgacgc cgcgtaccac ctgctggaaa
    22861 aaggagttaa taccgagcag caccagaccg ttaccgatta gcgtaatcac cagcacacca
    22921 agcaatgtac cgaacaggga accgcgaccg ccggaaagtg ccgtaccgcc gaccacgacc
    22981 gcggcgatga cgtcaaactc cagaccgttt gcggcacctg cgttaccgga accgaggcgt
    23041 gccgccaaca aaatgccggt caccgccgct aataatcccg aaagggtaaa gataagaatg
    23101 cgcactcgac gaacgttaat accgcacaac tgcgccgccg tggcattacc gccaacggca
    23161 aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca caaacagcgc aaacaacacc
    23221 atcatgatca gcgcggatac tggcacaccg agaaattgtc cgcccagcca gtccagcacc
    23281 tcgttttcgt caatcggcac tggcagtgcg ttggtcataa acagccccat tccgcgcaga
    23341 gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt taaacacgcc gcgcagcacc
    23401 ccggcgagcg ttcccatcag cgcgcctaac agcaacacca gcagacaagc aatcgccaac
    23461 ggaacttcaa attgcagcaa aaatgccagg cacaccgaga caaaagccac catcggccca
    23521 acgctgacat caatttcacc ggagataata atcagcgtca tcgcccaggc ggcaatccca
    23581 atggtggcgg catcgcgcag cacgttcatc tggttattca atgagataaa gccaggcgcg
    23641 ttcagggaga agaccagata aagaatggca atcaccacca gcaaaccgat ctcattaata
    23701 tggcgactga caaattgttt gagcgagacg ctcttgccct gcggcaatgg taatgacgaa
    23761 gcatacatct tcaggttcct cggtaaaatt catcagtgca cagacagaat ggcggacatc
    23821 agctcatcca cattgaccgg cgagtgaaac tcctgcgaga acgtgccgtg ctgtaataac
    23881 aggatgcggt cacacaccag cggcaattcc tccacttcac tggagataaa caccacgctt
    23941 tttccttcgg cagccagctc acggacaata cggtaaatct gctgtttggc ttcgatatcg
    24001 acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg cggcatagac ccaacgaccg
    24061 atcaccactt tttgctgatt gccaccagaa agcgtgccga tgggtgtttc gctactggca
    24121 gccttgaccg tcatccgctg catcacctct tcggtcaggc ggcggatggt ggaccattgc
    24181 agcacaccgt tggtgctgat tttttgccga ttggtcagca ctgtattttc gtcaacgcct
    24241 agccagggaa tgatccccgc ttctttgcgg ttttctggcg tatagccaat gccgcgtttc
    24301 agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt tgataacaat ttcgccctgt
    24361 tcatacgtct ccagcccaac aatcgccttc agcaattcac tgcgccctgc ccccagcagg
    24421 ccagcaatgc caagcacttc gccacgacgt agcgtaaagc tgatatcctc cagcttgggc
    24481 ttatggcgta acgcacggac ttccagtacg gcctgatcca caatttcctg aggggcaacc
    24541 ggcgcaatat caacgtgatc acgccctagc attagcgaca caatatgatg cgtggaggtg
    24601 ttttcgagca tcacatcgcc cgccacctga ccatcgcgca taacggtggc ataggaggca
    24661 atgcggcgga tttcttccat ccggtggctg acataaatca ccgccacgcc cagtgccgac
    24721 atctttttca ccgcactgat caccagttca acttctgcac tggcaagcga gctggtgggt
    24781 tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc gtgcaatttc caccagttgc
    24841 ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac taacgtcaac gcccagcgcc
    24901 tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat caatcatgcc gttgcggcga
    24961 ggccattgac caaggcagag gttttctgcc accgtcagcc cttccaccag acttaattcc
    25021 tgataaaccg cacgaacccc cagttcagcg gcacggcgag tcaacgtagc ttcgtcacct
    25081 tccagtcgcg tctcgccaat ccagatatca ccgctatccg ggcgttcgct gccggtaagc
    25141 attcgaatga gagtcgattt gcccgcgccg tttttaccta acagcgcacg aacttcgcct
    25201 ttattgagcg tgaagttaac gttatccagc gcaacgacgc cggggtaacg cttatttcct
    25261 gccaccactt ttgctaccgg gactgcctct gttgccgtga acatagggac ctctgcgaat
    25321 cagcgattag ggcagaccat caacgtgcgt tgccagccac tgtttaccat cttccgtttt
    25381 ggtatagaga tcgattggca cctgaatcac tttttcaccg tcggcttgtt tattgataac
    25441 cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta ccggaaatat ccactaccgc
    25501 tttcagcacc tgattgtttt ccagctcctg agcaatttcg gttgtcatat ccgaaccgaa
    25561 aacagcaatt tttccggcct gattttgatt acgtaccgct tttaccgcgc cgagtgtcgc
    25621 accgcccgac tcccccataa tggcgttgag atccggcgtg gagataatca gtttttcacc
    25681 aacggaaatc gctttatcta aaacagtccc ttcctggtta gcgacaattt gcgcgccggg
    25741 aacgcgggat tttaatactt cttcaaatcc tttacgtcgc tgcacacaaa cttcaaaggc
    25801 ttcgcaattg atgacggcaa ttttcggctg gtcaatttta ttggcaataa aataatcggc
    25861 ggcagcgtta cccagttttt taccaaattc cagtggatcg ccgaccagat acgccgagac
    25921 atatttatcg acaccctttt gattaataca ggtgttgtag caaatcaccg gaatacccgc
    25981 ttcactggcg cgacgaacgg tacggctact gccattttca gacactgccg ataaaataat
    26041 ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta ctttctttcg aaatatcccc
    26101 ctgggcgtta gtttcaatta actgtacctg tactgaagaa tcttttgccg catcctgaac
    26161 gccctgacgc actccagcgt aatatccctg ggtatcaagg tatattgcac caatggtcat
    26221 ttctttatcc gcagccctgg caaatagtgc gctacctaat agcgtagcca ttaataatag
    26281 attacgggtt gttctcattt ttttaggcat agtgcctcct gtagggtttt tattaacaac
    26341 ggcttattct aattattttg tgatgagcgg cagcgcgtgc cgctcaaata ttacagttaa
    26401 tagggtttag tgaacgtagt taaaaataaa aggcatcaca gtggtgaata atgccgcttt
    26461 cggccaggcc ggattaatcg ccgtcaattc agcgcgagca gactcatatt gcgcaacctc
    26521 cccgagtccc gcggacgcca gcatcgccac catcaggcat ttttctttat gctgctgttg
    26581 taaatcgcca tacagcgata acaggtcagg ttgtgaaaca gcaaagaaat ccgcctcaat
    26641 actggttttc gccatctctt gcgcccactg tttcatttca ctaaacagtt gctgtgcggt
    26701 ttgctgttca cccagcagtc gcagcgccat tccttgccag aagagataat caaccggctg
    26761 atcgttgtaa taactgtgga tgttaatggt gcgatcgccg gtcgccgcca gacgtaaaca
    26821 acgcatcgct tcagtttcat cgccctgcgc gttggcgcat atcgcctgcc agaaccagat
    26881 gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa ttctccggat aatacagcgc
    26941 ggcatgaagc agttcgctgg cctgctgcgg ctctctggca tcaagatgct gccaggcgcg
    27001 taataactgg ttgaggataa actgactggt gaccttccct tccccgcctt cccacgggtg
    27061 gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc tgacccgtga gatgccacaa
    27121 attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca atttccagat tattttccag
    27181 acgtgccagt cgtttctccg gtgtggctcc acttaactta tccagcaaat cccgttcgaa
    27241 aagcagacgt gcatcctgcg gcgcaagctg ataagcatta tcaagataac gcgcggccag
    27301 ctcataatcg tgtttcttat tccacgcatg gatcgctaac ccgcgccagc cgtcggcaaa
    27361 ctccggcgac atctctacgc aacgttgcca taaggcaatg gctttgttgt agctacgttt
    27421 gttgtagtag aagcaggcca gtaaatggcg agcaaaccag cactcttcaa tactctccag
    27481 cgccgccact tcttccagcg tattcgggaa acggacaaac tgcgggaaga catcaatggc
    27541 ttttgcgacc agttcgccac gttcggcttt cggcagcaag ctggcttgca ggtaaagcgg
    27601 cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc gccagggtgg gcattcccca
    27661 gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg cgtccctgac acagaccgcg
    27721 ccactgcgcg agggctgatt cgctacgacc atcgaaccag ttcagccacc acagagtggc
    27781 gttcagcgga taatcacgca gcagtttctc gcgctgcaaa cgcgcgttgt cctgacgacc
    27841 acttaacacc agcagcagat tatgcaagca aagcacttcc tgattggttg ggcaggcacg
    27901 aagactttgt tggcaaaaat ccagacccgc gtcgaagtta tcattacgcg ccgccagtcg
    27961 tgccagacca taatagccgc ctgctttact attgccgctc cagaccgcac gccagaaatc
    28021 ctcttcggct tgttgatatt gtccctgacg ttcgtaagca ctggcgcgaa tcaaacttgc
    28081 ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt ttcagcgcct gactggcata
    28141 cgccaccgct tgcgggaaat ctgcgcggtt atattccagc atcgccagcg ccaggttaca
    28201 gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag taatcgaacg gtgaacggct
    28261 ggcgtgatga tattgctcca gatgctgacc gataaaccag gcttcatctg tactggtaat
    28321 gtcttgtgct gccagtggcg ctttggcgac gtccggcaac ggcaactcct gcggctgatg
    28381 ttcctgataa ctcagtacaa tattgccgtc ggcatcggag agttcaatgg tcagtctgtc
    28441 aggattgata ccgtgcaaca cgccctggat ggcggtcgct ggtgtcaggg ccacggcatc
    28501 atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc agacggtatc cgttcaacgg
    28561 agagatggca tacagccccc actcaatccc ccgctcacta cgctggagtt ttatcaccgc
    28621 atcgcgggag gcattttgca ccatgcccag agaatgataa ggcaggaaat actgctcgaa
    28681 acgcttctct tcgtaagcat caagccaggt aaaatcaggc tggttatcgg caaaaatacc
    28741 ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga ctcttatccc acgcctggcc
    28801 aaattcactg tgtccccaac tccactgttt tttacctggc gcaatatggt ggttggcaac
    28861 gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg acaaaatcgt actgcgattt
    28921 ttcggccata tatgaggttg gaacaggcac atttttatag cgagaaatgt ccactccggc
    28981 ggagtagtcc actttgtagt aagtgccggt ggcgatgggg aaagcggaga cggcccgttt
    29041 gccgtgatca aacaccgcag tcacatccgg tgggaaaacg ctctgatgcc cttcaccccc
    29101 tttcactgcc gggttggccc accacaagaa atgacgcggc gtggcgttgc cgttatagac
    29161 gcggctggcg atttccagtg ccgcccggtc agggcgcagg gtgaaacctg tcatcacctg
    29221 taaaccatgc atcggctccg tttcgccgac ccacaccgtc tgtgcaccgt cttcatgggc
    29281 ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga tggtgttgcg gccagttaaa
    29341 ctcaatcccg ccggagatcc acggtcccag catccccacc agcgcaggtt taatgacttc
    29401 attgtgatag acaaaatcgc gctgtttcac tttatcccat gcgcgatgca cccgaccgcc
    29461 cagttccggc aggatcatca ctttgatgta gtcgttttcc agccacaccg cctgctagga
    29521 tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg tagggataaa ccgcgccgga
    29581 cgatccctga taaacgcgat tttccaggaa catgggatgt atatcctgcg gcccggtttc
    29641 ataggtcggg atctcaacgc gctcttgcca cacttttact ggagtcatgg tgccctcaat
    29701 attaacaaga catactgaat taaaagattt gtggcagtgt attgaacaat ctggcaatgt
    29761 tttcgcggaa taatcacgca attaactaaa caaggtttag tgaagatgag agcctgcatt
    29821 aacaatcaac agattcgcca ccataacaaa tgcgtgattc tggaactgct gtaccggcaa
    29881 aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa tttcgattcc ggcagtcagt
    29941 aatattttgc aggaactgga aagcgaaaaa cgggtggtga atattgacga tgaaagccag
    30001 acgcgcgggc atagtagcgg tacatggctg attgcgccgg aaggtgactg gacgctgtgc
    30061 ctgaacgtga cgcccaccag tattgagtgt caggttgcta atgcttgttt aagtccgaaa
    30121 ggcgaatttg agtatttaca gattgatgta ccgacgccgc aggcgctgct gtccgaaatc
    30181 gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc gcaccatcaa tctggcgctg
    30241 gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc aaaccatgcc gcaagcgccg
    30301 tggacaacgc cagttgaggt gaagtatctg ctggaagaga agctgggcat tcgggtgctg
    30361 gtcgataatg actgcgtgat gctggcgctg gcggagaaat ggcaaaataa ttcgcaggaa
    30421 cgggatttct gcgtgatcaa cgttgattac ggcattggct cgtcgttcgt gattaacgag
    30481 caaatttatc gcggcagttt gtatggtagc ggacagattg gtcacaccat cgttaatccg
    30541 gatggcgtcg tctgcgactg tggacgttac ggcagcctgg aaaccgtcgc ctcgttaagt
    30601 gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac cggttaatac tcagcttgag
    30661 cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc agagtggaga accgtggatc
    30721 accagctggg ttgatcgctc tgccaatgcc attggtttga gtctgtataa cttcctcaac
    30781 atcctcaata ttaatcagat ttggttgtac ggtcgcagtt gtgcctttgg tgagaactgg
    30841 cttaatacta ttattcgcca gacaggattt aacccgttcg accgcgacga aggaccgagc
    30901 gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg cacaacaggt gctgggaatt
    30961 ggctatttgt atgttgaggc gcagttacga cagatttgat ggcgcgataa cgtagaaagg
    31021 cttcccgaag gaagccttga tgatcataaa cgaaaaattg cctgatgcgc tacgcttatc
    31081 aggcctacac ggagattgca atatattgaa tttgcaagat tttgtaggcc tgagaaggtg
    31141 ttcatgccgc atccaacatg aacaacgagc acattgtcag caaatcaccg tttcgcttat
    31201 gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac ctttgatgcg ctcgataacg
    31261 gcttcatcat tgatgctgtc cagcacgtca cacatccagc cagccagttc tttcgcttcg
    31321 gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta cacgaatacc ggaggtcaca
    31381 aacgggctct tcggatcgtt cggtacgctg tttttgttga cggtgatgtt agcacggccc
    31441 agagcggcgt ctgcttcttt accggtcagg tttttatcaa ccagatcaac caggaacagg
    31501 tggttatcag tgccgccgga aaccactttg tagccgcgct cgaggaacac ttctaccatc
    31561 gctttagcgt ttttcgcgac ctgctgctgg taagttttga actcaggctc catcgcttct
    31621 ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg gaccgccctg accaccaggg
    31681 aaaacggcag agttcagttt tttgtacagc tcttcgctac cacctttcgc caagatcagg
    31741 ccgccgcgcg gacccgccag ggttttgtga gtggtggtag taacaacgtg agcatgagga
    31801 accgggttcg ggtagacgcc agcagcaacc aggcccgcaa cgtgcgccat atcaacgaac
    31861 agataagcac cgatgctgtc agcgatttca cgcattttcg cccagtcaac cacgccggaa
    31921 tatgcagaga aaccaccgat aatcattttc ggcttgtgtt ctttggcttg tttttccaga
    31981 tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag gaacgatgtt gtacagttta
    32041 ccggagaagt taaccggaga accgtgagtc aggtgaccgc catgcgccag gttcataccc
    32101 agaacggtat cacctggttc cagcagcgcg gtgtagaccg caaagttagc ctgggagccg
    32161 gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt ctttcgcacg atcgatcgcc
    32221 agttgttcaa cgatatcaac atactcgcaa ccgccgtagt agcgtttgcc cggataacct
    32281 tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca ttacgcgcgg gctggtgtag
    32341 ttttcggagg cgatcagttc gatgtgctct tcctgacgta ctttttcctg ctccatagcc
    32401 tgccacagtt cggcatcata atcggcaatg ttcatttcac gctttaacat ccgcatctcc
    32461 tgactcagct aacaataaaa tttttggcct ttataggcgg tcctgttgga caacggcgaa
    32521 cagtataacc gaatcattgt gcgataacag gtcttgacaa aggaatttac gcaaacgatt
    32581 accttcaggc tacgcaaggc tttggagaat aaagagcttg caaccggaaa cggatttctt
    32641 ttcaggtttg tgatgcaaat ttttcacttc atcacattct ttctgaaaaa caccaaagaa
    32701 ccatttacat tgcagggcta ttttttataa gatgcatttg agatacatca attaagatgc
    32761 aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct acagtaaaag ccaccatccc
    32821 tttactggtg gaaacggggc caaagttaac cgcccatttc tacgaccgta tgtttactca
    32881 taacccagaa ctcaaagaaa tttttaacat gagtaaccag cgtaatggcg atcaacgtga
    32941 agccctgttt aacgctattg ccgcctacgc cagtaatatt gaaaacctgc ctgcgctgct
    33001 gccagcggta gaaaaaatcg cgcagaagca caccagcttc cagatcaaac cggaacagta
    33061 caacatcgtc ggtgaacacc tgttggcaac gctggacgaa atgttcagcc cggggcagga
    33121 agtgctggac gcgtggggta aagcctatgg tgtactggct aatgtattta tcaatcgcga
    33181 ggcggaaatc tataacgaaa acgccagcaa agccggtggt tgggaaggta ctcgcgattt
    33241 ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc agcttcgaac tggagccggt
    33301 cgacggtggc gcagtggcag aataccgtcc ggggcaatat ctcggcgtct ggctgaagcc
    33361 ggaaggtttc ccgcatcagg aaattcgtca gtactctttg actcgcaaac cggatggcaa
    33421 aggctatcgt atcgcggtga aacgcgaaga gggtgggctg gtatccaact ggttgcacaa
    33481 tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg gcaggtgatt tctttatggc
    33541 tgtcgcagat gacacaccag tgacgttaat ctctaccggt gttggtcaaa cgccaatgct
    33601 ggcaatgctc gacacgctgg caaaagcagg ccacacagca caagtgaact ggttccatgc
    33661 ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt aaggaactgg ggcagtcact
    33721 gccgcgcttt accgcgcaca cctggtatcg tcagccgagc gaagccgatc gcgctaaagg
    33781 tcagtttgat agcgaaggtc tgatggattt gagcaaactg gaaggtgcgt tcagcgatcc
    33841 gacaatgcag ttctatctct gcggcccggt tggcttcatg cagtttaccg cgaaacagtt
    33901 agtggatctg ggcgtgaagc aggaaaacat tcattacgaa tgctttggcc cgcataaggt
    33961 gctgtaattt gatgttgccg gatggaaaca tccggcaacc cttgacgcgg tttaaattgc
    34021 cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga gccacgtcaa agacgaagat
    34081 tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata atggtatcga cacaggtatc
    34141 gacaatgtcg tccggtacga caatctcaat tttcactttc ggcagaaaat ccaccatata
    34201 ctccgcgccg cggtacagct cggtatggcc tttctggcga ccaaagcctt tcacttcggt
    34261 caccgtcatg ccggtaatac cgacttcggc cagtgcttcg cggacatcgt ccagcttgaa
    34321 gggttttata atcgcatcaa tctttttcat gctattcctt gaaaaggttg cctgtctttt
    34381 gatctgctaa acgtaacaca taacgccaat tcattccttg aaatcgtttg catccagctc
    34441 gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc cccgccattc tcgccgcgtg
    34501 ggtgacgttg cctttggtga tttgcagcag cttacgcaaa tagttgagtt caaaatgatt
    34561 acgtgcctca acaaaggttg gcagcgccgt attttcaccc tccagcgcct gctccaccag
    34621 cgcatcacta atcaccggag atgaggtcag cgccacgcac tgttcaatca cgttgaccaa
    34681 ctggcgcaca ttacccggcc agctcgcggt catcaggcgt ttcatcgcat cggtagagaa
    34741 cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc aacaggtgat ttgccagtag
    34801 cggaatgtct tctgtgcgct ccgccagtgc cggaattttc aggctgacaa cgttgaggcg
    34861 gtaatagaga tcttcgcgga attccccgcg cgccatcgct tttgacagat cacggtgagt
    34921 ggcagaaata atccgcacat tgatatcaat atcgcggtta ctgcccagcg ggcgcacttt
    34981 acgctcctgc aacacgcgca gcagtttgac ctgtaacggc gcaggcatat cgccaatttc
    35041 atcgagaaat agcgttccgc cttccgccgc ctggaataaa ccttcgcgat tgctgacagc
    35101 gccagtaaac gcgccacgcg catgaccaaa cagctccgac tccagcaatt gctcgggcaa
    35161 tgcgccacag ttaatagcaa taaatggttt gctgttgcgc gggctggcgt tgtggatagc
    35221 ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta atcaaaacgc tgacgtctga
    35281 ttgcgccacc agccgcgcct gttccagcaa acgcagcatc agcgggctgc gggtgacaat
    35341 tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc tccagcgcat cgtcaattgc
    35401 ctgatatagc gcatctttat cgacaggctt ggtgaggaaa ctaaaaacgc cctgctgtgt
    35461 tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga ataattactg gcattcccgg
    35521 ctgcactttc tggatttcag caaatagctg cataccgtcc atttcatcca tccgcaggtc
    35581 gctgataact aaatctactt tttcgcgatt cagtacccgt aatccttcag cgccactttc
    35641 cgccgtgacc acactgtagc cttcgctggt caggcgcagg ccaagcagtt tcagcaatcc
    35701 cagatcgtca tcgaccaata ataaatgcgc aggtttatgg ctcatcagga gtgacctcat
    35761 gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc cggcgagaaa tttccagccg
    35821 gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt ttccagcttg cgggtggtga
    35881 gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa tgtatccagc tcgctgtcgc
    35941 tggactgctg gagtttactg taacgttggc gctcttccgc cagttgcagt tgcagcgcct
    36001 gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc cgggatctcg gtacttaacg
    36061 cttcaatacg cgcgaccagc tggcggcgct catacggcgt aattttggcg tcggcgagca
    36121 aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc atattggcga gcttgctgac
    36181 gcgactgcgc aggcattaaa cgatcagcac aatccatcgc ccgcagccag taaagcggat
    36241 tggtttcggt tgatttacct tgcagcgccc agatgtcgct acattcagta gaaagatagt
    36301 cagccagttg ataaaccgga attttttctt ctgctggcgt atcaatggct ggcttattgt
    36361 gattctgcac gcaacccagc aatgccagac atggcagccc tgccagccac agccgtcggg
    36421 gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact taaagcctat cccagtgggc
    36481 gtaattgttg cagacagtct ggacatggac agcgcggaga aaccggggcg tacatatcgt
    36541 acgtgaagat ttcgagcact acccggggcc gaactgacaa ataaaatagc ctgataggat
    36601 aggctgttag cattatttcg tgtttttcga cgacggtaat tcaatgcgga aacaaacgtc
    36661 ttgcccgctc tcgtcgacca gatacagttc cccttgcata cggcgaatac aatccctagc
    36721 aatgcttaat cccagaccgc tgcccttcac cgcccctttt cgctggtggc ttccctgaaa
    36781 aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg ggcgtgcctg tattgatgac
    36841 atcaatataa acccgcgcac catgtaaact gctgcgaagg caaatgttac cggattcagc
    36901 cccgtagtgc accgcattgg agtaaagatt atccagtacg ctcatcagca gcattggctc
    36961 cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc atcattttag cccgtgcggg
    37021 caggctatga gcagaaacca ctgtctccac cagcggtgct aattcaacat tctccagttc
    37081 caccgcactg tccgcctgtt tacggttgta atcaagcagt tgttcgatca gtttttgcaa
    37141 attgcagctg ctgctatcaa gaatgctcac cacctctttt tgctctggcg taagcggccc
    37201 gacaacctga tcagccagta attcagtgcc ctcgcgcata ctcgccagtg gcgtttttaa
    37261 ttcatgagat aaatgtctta aaaattgatg gcgttgggat tccagccatg acaggcgctc
    37321 acttaaccaa agaatacgtt gcccaaccga gcgtaactcg ctcggtccac agaacgaaac
    37381 gctattgccc agagaacgcc cttcccccag acggttgatc atgcgctcga tatttttcac
    37441 cggcccgata atcatccgcg tgaaaagtag caccatcacc agactcacca gaaatagcac
    37501 cagcgattgc caaccaaaat attgcccacg ttcggcgatt tcacgctgaa gttgctgccc
    37561 acgagagaac accactgtgc gcgtggcctg caccatttcg gtattggcac tggcaaaggc
    37621 ttcaagacgc gcggcggcgg cagcatcggg accactgttg ttacactgaa gttgggccag
    37681 attgttcaaa tcctgacgca atgcctggta aagtttatcg tctggcagta cgcctgcgtg
    37741 ggcatcgagc atttcgctgt aacgcttgcg ctggctttga taaaccttcg caagcgtagg
    37801 gtcgtccagc acgcaatact gacggtagct acgctccatc tccagcgccg cgttggtcat
    37861 cgcttcacta cgccgggcat caataagcgt agtgcggtta accagcgccg cctgatcgct
    37921 aagcgcattc aggctttgcc aggctagcca tgccagcacc aacaggggta gtagaattag
    37981 caaaaatgcc agcattacca gttgtcgtaa tgagcgggga aaaacgggcc agcgtttcaa
    38041 ggtgttactc tcgtcagacg cgaatagcct gatgctaacc gaggggaagt tcagatacaa
    38101 caaagccggg aattacccgg ctttgttatg gaataaggcg gtgcctaact cgacgtttcg
    38161 cccgatggtt gatatagcta cgctgatatc agaagttgga cggcaggcac cttgttgtgc
    38221 gtcattcgaa ttttatgtag cacgtcccga aggggctgac ataagtcggt gaatgagcca
    38281 ctgcttacta ttatgcagta actgtgccaa taaagaaaat agtttggtaa cgtactgatt
    38341 atacgttgct ttgagggatt tatggcttct tcgctatttg aatgtaaatc agccactgtg
    38401 tcgctaaaaa gagacaactt aagataaact tattagatta caataaaatc aatcatttaa
    38461 gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt gatttacaga cacaaaaaag
    38521 ctcccggagt tgggagctta tgatagtggt tggtgcttaa tgccggatgc ggcgtaaatg
    38581 ccgcatccgg gctgcaaaac caatgaactg atgacttacc ccaactgctt acgcgcattg
    38641 cggaaaatgc gcatccatgg gccatcctcg ccccagtttt ccggatgcca ggagttgctg
    38701 acagtacgga aaacacgttc cgggtgcggc atcataatgg tgactcgacc actttcagtc
    38761 gtaaccgccg taataccgtt cggtgaaccg ttcgggttag ccgggtaggt ttcagtgact
    38821 ttgccgaagt tatcgacata gcgcagtgcc accagccctt tgctttccag tgccgccaga
    38881 tgcgccgcat cacgcacttc cacgcgccct tcaccatgag agacagcaat cggcatttgc
    38941 gagcccacca tcccctgcaa cagcagagac gggctttggg ttacttcaac caggctgaaa
    39001 cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg gccacaactc actccccggg
    39061 atcagttcac gcaggttaga catcatctgg caaccgttac atacccccag ggccagcgtt
    39121 tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac ggtcattgaa caggatagac
    39181 ttcgcccaac cttcaccggc acccagcaca tcaccgtagg agaaacctcc gcacgcgacc
    39241 agggcgtgaa aatcctccag tcccgtacgt ccagtcagca ggtcgctcat atgcacgtcg
    39301 atagcatcaa agcccccacg ttggaaagct gccgccattt caacatgcga gttcacgccc
    39361 tgctcacgca gtacggcaac tttcggacgt gcgccagtgg caatatacgg tgctgccaca
    39421 tcttcgttga tatcgaacga cagttttaca ttcaggcccg gatcggcgtc gttagatttc
    39481 gcctgatgct cctgatcggc acactccggg ttgtcacgca ggcgctgcat ctgccaggta
    39541 gtttctgccc accagacacg caacgtagtg cggctttcgc tgaatacagt ctgcccgttg
    39601 gcggtaatca caaaacggtc accggaaacc gcctgcccaa cataatggac gcaatccgca
    39661 agcccatgct gtgccagtac ggactcgacc gcttcacggt cagcggcacg aacctgaatc
    39721 accgcaccca gttcttcgtt aaacaacgcc gccaggcgat cgtcacccag agaggcgata
    39781 tccgcgtcaa tgccacaatg acctgcaaag gccatttccg ccagcgttac cagcaggcct
    39841 ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg caaccagcgc ctgaatcgcg
    39901 tcatagaagc ctttcagttg gacaacatcg cgtacatctg ccggtttgtc gccaagctga
    39961 cgataaacct gcgccagcgc cgttgcgccc agcgcgttat tgcctttgcc caaatcaatc
    40021 agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga tggtgtgacg tacatcttcc
    40081 acgcgggcaa atgcagaaat caccagcgac agcggcgacg tcatttcgcg ctcttcgtta
    40141 ccttcctgcc agcgggtttt catcgacata gagtctttac ccaccgggat cgtcaggccc
    40201 agcgccggac aaagttcttc acccacggct ttaacggctt catacaggcc cgcatcttca
    40261 ccagggtggc ctgccgccgc catccagttg gcggaaagtt taatgcgttt gatatcgcca
    40321 atctgtgttg cggcaatatt ggttaacgct tcaccgaccg ccagacgggc agaggcggcg
    40381 aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca tcgcttcacc gtagtagctg
    40441 tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca cctgccacgg ccccaccatc
    40501 tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga tggtcaccag gaaagttttc
    40561 tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt ctgcaatggt gatcccttca
    40621 cggaccagcg catcgccctt cgctttcagc gtttgtacat cgcgggtcat cttcggcgtt
    40681 ttaccaagca ggacgtccag cggcagatcg atcggctgat tatcaaaatg acgatcgtgc
    40741 agagaaagat gcagttcttc ggtcgcttca ccaatcaccg cgtagggtgc gcgctcacgc
    40801 ttacacagtt cgtcaaacag cggtaattga tcggcagcaa ccgccagcac gtagcgttcc
    40861 tgggattcgt tacaccagat ttccagcggg ctcatgcccg gttcgtcgct tagaatatcg
    40921 cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca gctccggcat cgcgttagaa
    40981 agaccgcccg caccgacgtc gtggataaac agaattgggt tagcatcacc aagctgccag
    41041 caacggtcga tcacttcctg gcaacgacgc tccatctccg ggttgtcgcg ctgtacggaa
    41101 gcaaagtcga ggtcggcatc agactgacca gacgccatgg aagacgccgc gccgccgcca
    41161 agaccgatgt tcattgccgg gccgccgaga acgaccagct tcgcaccgac gttgatctcg
    41221 cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc ccgccagcat gatcggtttg
    41281 tgataaccgc gcagctcttc gccattgtgg ctgttcactt tttcttcata agtacggaag
    41341 tagccgttca gtgccggacg accaaattcg ttgttaaacg ccgcaccgcc cagcgggcct
    41401 tcggtcatga tgtccagcgc ggtaacaatg cgctcaggct taccgaaatc ttcttcccac
    41461 ggctgttcga agccaggaat tcgcaggttg gatacggaga aaccaaccaa accagctttc
    41521 ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt caccgccgga accggtcgcc
    41581 gcgcccggcc acggagaaat cgccgtcggg tggttgtgag tttcgacttt catcaggatg
    41641 tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt ggtcagcaaa gtagcggccc
    41701 acttcagaac cttccattac ggcggcgtta tctttataag cagagagaac gtgatctggc
    41761 gtggtttcga aagtattttt gatcatcctg aacagcgatt tcggctgttg ttcaccgtcg
    41821 ataatccagt cggcgttaaa aattttgtgg cggcagtgct cagagttcgc ctgagcgaac
    41881 atatacagct cgatgtcgtt cgggttacga ccaagctttg tgaaagcatc ctgcagatag
    41941 tcaatttcat cttccgccag agccaagcca agacgcaggt tagcgtcgat cagcgcctga
    42001 cggccctgcc ccagcaaatc aacgcttgtg accggagtcg gttgatggtg ggcaaacaac
    42061 tgctctgcat catctaaatc aaaaaagacc gtttccatca tgcggtcgtg cagttcagcg
    42121 gtaacctgct gccattgttc attggtcagc gtaccggctt ctatatagta agcaacgccg
    42181 cgctcaaggc ggtttacctg ttgtagcccg cagttatggg caatatcggt cgctttcgaa
    42241 gaccagggag agatggtgcc aggacgcggg gtcaccagca ggagtttgcc ttgcggggcg
    42301 tggctggcga gtgccggacc atatttcagc aggcgttcaa gttgtgcgtg ctcatcatcg
    42361 tttaacgacg cattgaggtc agcaaaatgg acatactcgg cgtaaatatt gtgaaccggg
    42421 agcctggcag cctgaaaacg tgccagcagt ttgttgattc ggaatgccga cagtgcaggc
    42481 gaaccacgca gaatttccat cataagtctc tcgtcttcta agctttcggt gtacccaagg
    42541 ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg acgaaaccgt ttgcgtggaa
    42601 ataaaatcac catcgtgaat tagcaacgcg tgccgccaat ggctgtaata agttgccatc
    42661 tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa acctttcact attattacgt
    42721 tttttcaagc tgggacgcgc acgacacaga gaattaacta attgaaaaaa ttaaagatta
    42781 attatctgtt catcggcatt ctggcactgc tgctcgcggt cgctctctgg ccatccattc
    42841 cctggtttgg taaagccgac aaccgtatcg ccgccattca agcgcgggga gagttgcgtg
    42901 tgagcaccat tcatactccc ctgacttata acgaaatcaa cgggaaacct tttggcctag
    42961 attacgaact ggcgaaacag tttgccgatt acctcggcgt aaaactgaaa gtgaccgtgc
    43021 ggcagaatat cagccagctg tttgacgacc ttgataatgg taacgccgac ctgctggcgg
    43081 caggactggt ctataacagt gagcgggtaa aaaattatca gcctggccct acctattatt
    43141 ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg cccacgtacg ctgggcaacc
    43201 tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt ggttaacgat ctccagaccc
    43261 tgaaagaaac aaaattcccg gaattaagct ggaaggtaga cgacaaaaaa ggctctgcgg
    43321 aattaatgga agatgtcatc gaaggaaaac tcgattacac cattgctgat tctgtcgcca
    43381 tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc gctcgatatc accgatgaac
    43441 aaccggtgac ttggtttagc ccgttagatg gcgataatac cctttccgcc gccctgctcg
    43501 acttcttcaa cgaaatgaat gaagacggta cgctggcacg cattgaagag aaatacctgg
    43561 ggcatggcga tgattttgat tacgtcgata cgcgcacatt tttacgcgcc gtcgatgcgg
    43621 tactgccgca gttaaagccc ctgtttgaga aatacgccga agaaattgac tggcgtttgc
    43681 tggccgctat tgcttatcag gaatcgcact gggatgcaca ggccacttca ccgacgggtg
    43741 tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag cctcggcatt acggatcgta
    43801 ccgatgccga acagagcatc agcggtggcg tgcgttattt gcaggatatg atgagtaaag
    43861 tgccggaaag tgtgccggag aacgaacgga tctggtttgc cctcgctgcg tacaatatgg
    43921 gctatgcgca tatgctggat gcccgcgccc tgacggcaaa aaccaaaggg aatcctgaca
    43981 gttgggctga cgtaaaacag cgtctgcctt tacttagcca gaaaccctat tacagcaagc
    44041 tgacttacgg ctacgctcgt gggcatgaag cctacgctta tgtcgaaaat attcgtaagt
    44101 atcagattag cctggtgggt tatctgcaag agaaagagaa gcaggctaca gaagcggcga
    44161 tgcaactggc gcaggattat ccggcggtat cgcctacgga gttgggcaaa gagaaatttc
    44221 cttttctctc gtttctttcc cagtcgtcat caaactattt gacccattct ccctctctgc
    44281 tgttttccag gaaagggagt gaagagaaac aaaattaatc cgtcgaggat tgcgcttttt
    44341 tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc actgagcaac gccgcgcact
    44401 catccgccag tattccttcc gtaatttcca ctcggtgatt catacccgga tgatgcagca
    44461 catccattaa agatcccgca gcgccagttt tcgcgtcacg cgcaccaaag accacgcgac
    44521 caatgcgact gtggatcatc gctccggcac acattacaca tggttcaagc gtgacataca
    44581 acgtggcgtc gatcagacga taattttgca tcaccagacc accctgccgc agggccatga
    44641 tttctgcatg tgcggtggga tcatggcgac caatcgggcg gttccagcct tcgccgatta
    44701 cccgattgtt atgcactaat accgcgccga ccggcacttc ccgctcatcc caggcacgtt
    44761 tcgccagcgt cagcgcgtga cgcatccaat attcgtggct aaattcgact tcagacaaaa
    44821 agaaaactcc ggttataaaa gcgcggcgca ttatacacgg acgctatgct ttactctatt
    44881 ccagttgctg gagttcaccg cgcggggtta ctcgccaacg atgctgacag aaataaagca
    44941 gcggattgtc ctgattactg tcgctatagc cactgtacag ccgcagcggc gtgccgattt
    45001 tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg catcgtcaat acccaaccac
    45061 catagccacg ctgaatttgg ctggcgataa gattaacccg cggcagccag ggcgtatcga
    45121 aataaaccgc ttcaaccagc ggctgcggag agccggtaat caaccagata tcagcatcgg
    45181 aacttaacag gtaggtggtt aatcgctcct gaaccagcgg aaaggcggta acattgtcgc
    45241 gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg tgcttcgctg tgaccaaaag
    45301 tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc acgacctttt atcaataacg
    45361 caatggctat aatcggtaac aacggcagga caagtaacgc attcagcggt tggcgacgca
    45421 gtaaatagcg cagaaaactg ccgaacatat cctgctgatg caatgttcca tctaagtcaa
    45481 aaaacaccac acgacgctcg tgagttgcca aaccatactc ctctggactg aaacatcctg
    45541 attaattttc gctactacat agcctaacag atagatcatc acttttccgg caacaatcga
    45601 ccgcatcatg gctaactgga atttttaatt catggcaatt agcggcaatg gaatataaaa
    45661 ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta tttcatcgcg aggaaagata
    45721 tgaacggctt acttcgtatc cgtcagcgtt accaagggct tgcccagagt gataaaaaac
    45781 tggcggatta tctgctgcta caacctgata cggcgcgcca tttaagctct cagcaactgg
    45841 ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt cgcgcaaaaa ctcggctata
    45901 aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct ggcaagccag ccggaatcgc
    45961 cctccgtgcc cattcataac caaatccgcg gtgatgatcc gttacggctg gtcggcgaaa
    46021 aactgattaa agaaaatact gccgctatgt acgcaacgct aaacgttaat agtgaagaga
    46081 aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg gataattctg accggtattg
    46141 gcgcttcggg tctggtggcg caaaactttg cctggaagct gatgaagatt ggcttcaatg
    46201 ctgccgcact gcgcgatatg catgcgctac tcgcaacagt acaggcgtcg tcccctgacg
    46261 atctgttatt agccatttcc tacaccggtg tacgacgcga gttaaacctg gcggcagatg
    46321 agatgctgcg agtgggcgga aaagtgctgg cgattaccgg ctttactccg aatgccctgc
    46381 aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga acaagcgaca aacagtgctt
    46441 caatctctgc ttgtcacgct cagggaatgt taacggattt gctgttcatt gcgctgattc
    46501 agcaggatct ggaactggca ccagaacgta ttcgtcatag tgaagcgctg gtgaaaaaac
    46561 tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt tgtgttgttt tgagagtttc
    46621 cttatggcgc tgttaatcac taaaaaatgc atcaattgtg atatgtgtga aatcaaataa
    46681 aaacacattt attttcatag tgatacagca atgtgttatt gaatgtgttg ttggatttat
    46741 ggtgcaaaaa aattttgcat aagttcagca acacaaagaa caccactttc tttaacgtcc
    46801 tccgcgccct aaaggacggg gctttacggc gcaaaaggcc tgtgacatac aacattaact
    46861 tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct gcggttttat ataaccctgg
    46921 ctggaaacct cttatacaaa gtcgattcgc caacatcata gatgatcgcc accttctggc
    46981 gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca ttattctggt gaaagtttgg
    47041 gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc cagtcctgct tttgttcgtt
    47101 caacaatcag ttcacgttcc atttcagcca gggcacccat cacatggaag aaaaagcgtc
    47161 ccattggtgt gctggtatca attgaatcag tcagactacg aaagttgatg cctcgttcgc
    47221 gcaactcctc caccagcacg acaagatgcc gcatactgcg ccccagccga tccagcttcc
    47281 agacaaccag tgtgtcgcct gccgataatg tcttgagcag ttttttcagc cccggtcttt
    47341 cggactttgt accacttatc ttgtcttcaa aaatcagctc gcatcctgca cagttcaacg
    47401 cattacgttg tagatcggta ttctggtcat ttgttgatac gcgtacatag ccaataagca
    47461 tggtaaatcc ccctgttaac aagacactgc gtcaaaagtc gg
//
LOCUS       DAJTRB010000022        47226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTRB010000022 DAJTRB010000000
VERSION     DAJTRB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.022"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            28..594
                     /gene="satP"
                     /locus_tag="OO330_001327"
     CDS             28..594
                     /gene="satP"
                     /locus_tag="OO330_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5882257.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(629..1216)
                     /gene="mog"
                     /locus_tag="OO330_001328"
     CDS             complement(629..1216)
                     /gene="mog"
                     /locus_tag="OO330_001328"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5882258.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(1331..2284)
                     /gene="tal"
                     /locus_tag="OO330_001329"
     CDS             complement(1331..2284)
                     /gene="tal"
                     /locus_tag="OO330_001329"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5882259.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2563..3993
                     /locus_tag="OO330_001330"
                     /pseudo
     CDS             2563..3993
                     /locus_tag="OO330_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            4063..4839
                     /gene="yaaA"
                     /locus_tag="OO330_001331"
     CDS             4063..4839
                     /gene="yaaA"
                     /locus_tag="OO330_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5882260.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4992..5288)
                     /locus_tag="OO330_001332"
     CDS             complement(4992..5288)
                     /locus_tag="OO330_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5882261.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(5502..6788)
                     /gene="thrC"
                     /locus_tag="OO330_001333"
     CDS             complement(5502..6788)
                     /gene="thrC"
                     /locus_tag="OO330_001333"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5882262.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6789..7721)
                     /gene="thrB"
                     /locus_tag="OO330_001334"
     CDS             complement(6789..7721)
                     /gene="thrB"
                     /locus_tag="OO330_001334"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5882263.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7723..10185)
                     /gene="thrA"
                     /locus_tag="OO330_001335"
     CDS             complement(7723..10185)
                     /gene="thrA"
                     /locus_tag="OO330_001335"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5882264.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(10211..10330)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(10266..10331)
                     /gene="thrL"
                     /locus_tag="OO330_001336"
     CDS             complement(10266..10331)
                     /gene="thrL"
                     /locus_tag="OO330_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5882265.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(10545..11231)
                     /locus_tag="OO330_001337"
     CDS             complement(10545..11231)
                     /locus_tag="OO330_001337"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5882266.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11631..11771)
                     /locus_tag="OO330_001338"
     CDS             complement(11631..11771)
                     /locus_tag="OO330_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882267.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDSTVSKTAVILTILT
                     AT"
     gene            11867..12583
                     /gene="arcA"
                     /locus_tag="OO330_001339"
     CDS             11867..12583
                     /gene="arcA"
                     /locus_tag="OO330_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5882268.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12642..13994)
                     /gene="creD"
                     /locus_tag="OO330_001340"
     CDS             complement(12642..13994)
                     /gene="creD"
                     /locus_tag="OO330_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5882269.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(14052..15476)
                     /gene="creC"
                     /locus_tag="OO330_001341"
     CDS             complement(14052..15476)
                     /gene="creC"
                     /locus_tag="OO330_001341"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5882270.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVSLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(15476..16165)
                     /gene="creB"
                     /locus_tag="OO330_001342"
     CDS             complement(15476..16165)
                     /gene="creB"
                     /locus_tag="OO330_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5882271.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(16178..16651)
                     /gene="creA"
                     /locus_tag="OO330_001343"
     CDS             complement(16178..16651)
                     /gene="creA"
                     /locus_tag="OO330_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5882272.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16862..17731
                     /gene="robA"
                     /locus_tag="OO330_001344"
     CDS             16862..17731
                     /gene="robA"
                     /locus_tag="OO330_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5882273.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17728..18375)
                     /gene="gpmB"
                     /locus_tag="OO330_001345"
     CDS             complement(17728..18375)
                     /gene="gpmB"
                     /locus_tag="OO330_001345"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5882274.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            18427..18947
                     /gene="yjjX"
                     /locus_tag="OO330_001346"
                     /pseudo
     CDS             18427..18947
                     /gene="yjjX"
                     /locus_tag="OO330_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(19032..19358)
                     /gene="trpR"
                     /locus_tag="OO330_001347"
     CDS             complement(19032..19358)
                     /gene="trpR"
                     /locus_tag="OO330_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5882275.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(19448..21385)
                     /gene="sltY"
                     /locus_tag="OO330_001348"
     CDS             complement(19448..21385)
                     /gene="sltY"
                     /locus_tag="OO330_001348"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5882276.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGHSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            21596..23263
                     /gene="ettA"
                     /locus_tag="OO330_001349"
     CDS             21596..23263
                     /gene="ettA"
                     /locus_tag="OO330_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5882277.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(23370..23537)
                     /locus_tag="OO330_001350"
     CDS             complement(23370..23537)
                     /locus_tag="OO330_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882278.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(23571..24803)
                     /gene="nadR"
                     /locus_tag="OO330_001351"
     CDS             complement(23571..24803)
                     /gene="nadR"
                     /locus_tag="OO330_001351"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5882279.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24824..26206)
                     /gene="radA"
                     /locus_tag="OO330_001352"
     CDS             complement(24824..26206)
                     /gene="radA"
                     /locus_tag="OO330_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5882280.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(26255..27223)
                     /gene="serB"
                     /locus_tag="OO330_001353"
     CDS             complement(26255..27223)
                     /gene="serB"
                     /locus_tag="OO330_001353"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5882281.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            27329..27973
                     /locus_tag="OO330_001354"
     CDS             27329..27973
                     /locus_tag="OO330_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5882282.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            28001..29016
                     /gene="lplA"
                     /locus_tag="OO330_001355"
                     /pseudo
     CDS             28001..29016
                     /gene="lplA"
                     /locus_tag="OO330_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            29048..29311
                     /locus_tag="OO330_001356"
     CDS             29048..29311
                     /locus_tag="OO330_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5882283.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(29472..30191)
                     /gene="deoD"
                     /locus_tag="OO330_001357"
     CDS             complement(29472..30191)
                     /gene="deoD"
                     /locus_tag="OO330_001357"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5882284.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(30248..31471)
                     /gene="deoB"
                     /locus_tag="OO330_001358"
     CDS             complement(30248..31471)
                     /gene="deoB"
                     /locus_tag="OO330_001358"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5882285.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(31523..32845)
                     /gene="deoA"
                     /locus_tag="OO330_001359"
     CDS             complement(31523..32845)
                     /gene="deoA"
                     /locus_tag="OO330_001359"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5882286.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32972..33751)
                     /gene="deoC"
                     /locus_tag="OO330_001360"
     CDS             complement(32972..33751)
                     /gene="deoC"
                     /locus_tag="OO330_001360"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5882287.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            34009..35550
                     /locus_tag="OO330_001361"
     CDS             34009..35550
                     /locus_tag="OO330_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5882288.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            35522..36385
                     /locus_tag="OO330_001362"
     CDS             35522..36385
                     /locus_tag="OO330_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5882289.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(36599..37381)
                     /locus_tag="OO330_001363"
     CDS             complement(36599..37381)
                     /locus_tag="OO330_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5882290.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAIHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(37378..38451)
                     /locus_tag="OO330_001364"
     CDS             complement(37378..38451)
                     /locus_tag="OO330_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5882291.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(38573..38734)
                     /locus_tag="OO330_001365"
     CDS             complement(38573..38734)
                     /locus_tag="OO330_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5882292.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38851..39456)
                     /gene="osmY"
                     /locus_tag="OO330_001366"
     CDS             complement(38851..39456)
                     /gene="osmY"
                     /locus_tag="OO330_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5882293.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39849..41438)
                     /gene="prfC"
                     /locus_tag="OO330_001367"
     CDS             complement(39849..41438)
                     /gene="prfC"
                     /locus_tag="OO330_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5882294.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(41529..42206)
                     /gene="yjjG"
                     /locus_tag="OO330_001368"
     CDS             complement(41529..42206)
                     /gene="yjjG"
                     /locus_tag="OO330_001368"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5882295.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(42221..42667)
                     /gene="rimI"
                     /locus_tag="OO330_001369"
     CDS             complement(42221..42667)
                     /gene="rimI"
                     /locus_tag="OO330_001369"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5882296.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42636..43049)
                     /gene="holD"
                     /locus_tag="OO330_001370"
     CDS             complement(42636..43049)
                     /gene="holD"
                     /locus_tag="OO330_001370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5882297.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            43152..44183
                     /gene="rsmC"
                     /locus_tag="OO330_001371"
     CDS             43152..44183
                     /gene="rsmC"
                     /locus_tag="OO330_001371"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5882298.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            44460..44546
                     /locus_tag="OO330_001372"
     tRNA            44460..44546
                     /locus_tag="OO330_001372"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44494..44496,aa:Leu,seq:cag)
     gene            44575..44661
                     /locus_tag="OO330_001373"
     tRNA            44575..44661
                     /locus_tag="OO330_001373"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44609..44611,aa:Leu,seq:cag)
     gene            44696..44782
                     /locus_tag="OO330_001374"
     tRNA            44696..44782
                     /locus_tag="OO330_001374"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44730..44732,aa:Leu,seq:cag)
     gene            complement(44821..45057)
                     /locus_tag="OO330_001375"
     CDS             complement(44821..45057)
                     /locus_tag="OO330_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5882299.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            45198..45986
                     /gene="fhuF"
                     /locus_tag="OO330_001376"
     CDS             45198..45986
                     /gene="fhuF"
                     /locus_tag="OO330_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5882300.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(46024..46701)
                     /gene="bglJ"
                     /locus_tag="OO330_001377"
     CDS             complement(46024..46701)
                     /gene="bglJ"
                     /locus_tag="OO330_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5882301.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(46659..>47226)
                     /locus_tag="OO330_001378"
     CDS             complement(46659..>47226)
                     /locus_tag="OO330_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5882302.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNIAELRREMSHS"
BASE COUNT        11155 a        12852 c        11895 g        11324 t
ORIGIN      
        1 tcattcgatg atgatttttg aggaattatg ggcaacacta agttggctaa tccggcaccg
       61 ctgggcctga tgggcttcgg catgaccacc attctgctta acctgcacaa cgtgggttat
      121 ttcgctctgg acggtattat tcttgccatg ggcattttct acggcggcat cgcgcaaatt
      181 tttgctggtc tgctggagta caaaaaaggc aacactttcg gtttaaccgc attcacctct
      241 tacggttctt tctggctgac gctggttgcg attctgctga tgccgaaact gggtctgacc
      301 gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc tgtggggcgt atttacgctg
      361 tttatgttct tcggcactct gaaaggcgcg cgcgtcctgc aattcgtttt ctttagcctg
      421 accgtgctgt ttgccctgct ggcgatcggt aacattgccg gtaatgccgc gatcatccac
      481 tttgccggct ggattggtct gatctgcggt gccagcgcaa tctatctggc gatgggtgaa
      541 gtgctgaacg agcagtttgg tcgcaccgtt ctgccgattg gtgaatccca ctaattgtct
      601 tgagatcccc ccgctcgggg ggattttttt attcgctaac gtcgcgtctt gcactcttcg
      661 gtctgaatgc agcaaccact tctggtgccg tttcaacgta tggcccttcc agcaactgaa
      721 tgcagtacgg tacgctggca aaaataccat gcaccacaac gttaccctca gcgtccttca
      781 caccttccag cgtctcttta atagacttcg gctgaccggg taagttaagg atcagcgcct
      841 gtttgcgaat cacgcccacc tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctga
      901 tctggcgcat ctgttcacca aagccaggca tctcgcggtc cgctactgcc agcgtcgcat
      961 cgggcgttac gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc agatggcaac
     1021 tcatttcatc caccagctca cacaacgttt gctcgatgat cgcctgctca tcggggatta
     1081 agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt cagccattct tccagcgctg
     1141 gaatgccttt atcctgataa acgccgctgg atgcgcgatc agagatggaa actaagccaa
     1201 tacgtaaagt attcatcaag atttttccgt ttaaacaatc tcgttaaaca gaatgataca
     1261 ctgcgaaggg agtgacagac aggcttcaga agaggtagcg tgaccgactt cccggtcacg
     1321 ctaagaatga ttacagcaga tcgccgatca ttttttccag tttttcctgg tcaatagcaa
     1381 acttacggat accttccgcc agtttatcta ctgccattgg atcctggttg tgctgccaca
     1441 ggaactcgga ctcagtgata cgcgccggac gcgctttcac ttcgccggtg taagacagtt
     1501 tacgttcgat agccccttcg ctctccgcca gctctttcag cagtgccggt gcgatggtca
     1561 gacggtcaca gcctgccagt tccagaattt cgccgatgtt acggaagctt gcgcccataa
     1621 ccacggtttc ataaccgtgc tctttgtagt actggtagat ttcagataca gaaaccacgc
     1681 ccggatcttc tgccggagcg tactctttct tatcggtatt cgctttgtac cagtcaagaa
     1741 tacggccaac aaacggcgag atcaggaaca cgcccgcctc cgcacaagca cgagcctgag
     1801 cgaaggagaa cagcagggtc aggttacagt tgataccttc tttttccagc tgttctgcag
     1861 cacggatacc ctgccaggta gaagccagtt tgatcagaat acgatcgttg ctaatacctg
     1921 catcgttgta gagtttgatc aggcgttttg ctttcgcaat tgacgcttcg gtgtcatagg
     1981 aaagacgcgc atcaacttca gttgagatac ggcccggaac cagtttcagg atttccagac
     2041 caatatttac tgccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct
     2101 gctgtttcgc ccaggcaaca gcatcatcaa tcaacttacg gtattccgga atctgcgctg
     2161 cgttaagaat gagagaaggg ttggttgtgg catcctgcgg ttgatacagc ttcattgccg
     2221 cgatgtcccc agtgtcggcc actacggtgg tgtactgacg aagggaggtc aatttgtccg
     2281 tcatgatagt atttctcttt aaacagcttg ttagggggat gtaaccggtc tgccctgatg
     2341 atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt
     2401 ttcggatttc tgacactgcg ttgatatcgc ccaccatttt tatacaaaac ctcatgtatg
     2461 ctacgcagaa gttatcaagt acctcgtagc gtatatactt cttaaacaat tggtaacgtt
     2521 tacacaggaa agtcatcgcg accggcaata agagggatat gcatgccaga ttttttctcc
     2581 tttattaaca gcgtcctttg gggatcggta atgatttacc tgctcttcgg cgcaggttgt
     2641 tggttcactt ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt tggcaaaagt
     2701 cttaaaaata gcattcatcc acagccaggc ggtttaacct catttcagtc attgtgtacc
     2761 agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc aattaccgcc
     2821 ggtggaccag gtgccgtctt ttggatgtgg gttgctgcgt ttatcggcat ggcgacctcg
     2881 tttgccgaat gttcccttgc acaactttat aaagaacgtg acgtcaatgg gcagtttcgt
     2941 ggcggaccgg catggtatat ggcgcgcggg ctggggatgc gctggatggg cgttctgttc
     3001 gccgtctttt tgctcatcgc ctacggcata attttcagcg gagttcaggc gaacgccgtt
     3061 gcccgagccc tgagtttttc ttttgatttt cctccgctgg tgacgggcat tattctcgct
     3121 gtctttgctc tgctggcaat cactcgcggt cttcatggcg tcgcccggct catgcagggg
     3181 tttgtcccgt tgatggcgat aatctgggta ctgaccagcc tggtgatttg cgtaataaat
     3241 atcgggcaac ttccccacgt catttggtct atttttgaga gtgcttttgg ctggcaggaa
     3301 gcggcaggcg gcgcggcggg atatacctta agccaggcga ttactaacgg ttttcagcgc
     3361 agtatgtttt ccaatgaggt gggaatggga tcgacgccaa acgcggcagc ggcagctgca
     3421 tcctggcctc cgcatccggc agcacaaggg attgtccaga tgattggcat ttttatcgac
     3481 accctggtca tctgtacggc aagcgccatg ctgatattac tggcgggtaa cggcacaacc
     3541 tacatgccgc tggaaggtat tcagcttatc cagaaggcga tgcgggtgct aatgggttcc
     3601 tagggtgctg aatttgttac cctcgtggtt attctgtttg ccttcagctc catcgttgcc
     3661 aactacattt atgccgaaaa caatctcttc tttttacgcc tgaacaaccc taaagcgatc
     3721 tggtgtttgc ggatctgcac cttcgcaacg gtcatcggcg gcaccttgct aagccttccg
     3781 ctgatgtggc aactggcaga tatcataatg gcctgcatgg ctattaccaa tttgaccgcc
     3841 attttactgc tctcgcctgt ggttcatacc attgccagtg attatctacg ccagcgtaaa
     3901 ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg atatcggtcg ccagctttct
     3961 ccggacgcgt gggatgatgt ttcgcaggag taatcacaac tatcgatcaa ctcattctct
     4021 ttttttgcta aagtcggcat aaatttcctg caaggactgg atatgctgat tcttatttca
     4081 cctgcgaaaa cgcttgatta ccaaagcccg ttgaccacca cgcgctatac gctgccggag
     4141 ctgttagaca attcccagca gttgatccat gaagcgcgga aactgacgcc tccgcagatt
     4201 agcacgctga tgcgcatcag cgacaaactg gcgggtatca acgccgctcg ctttcatgac
     4261 tggcagccag atttcacgcc ggcgaatgcc cgccaggcga ttctggcgtt taaaggtgat
     4321 gtctacaccg gcttgcaggc cgaaaccttc agcgaagacg atttcgattt tgcccaacag
     4381 catttgcgaa tgctttccgg cttgtatggc gtactgcgcc cgctcgattt aatgcagcct
     4441 tatcgtctgg aaatggggat ccgtcttgag aatgcccgag ggaaagatct gtatcaattc
     4501 tggggagata tcatcaccaa caagctgaac gaggcgctcg cagcacaagg cgataatgtg
     4561 gtgattaatc tggcgtcaga tgagtatttt aaatcggtga agccgaagaa attgaatgcc
     4621 gagattatca agccggtgtt cctcgatgag aagaacggca aatttaagat catcagcttc
     4681 tacgctaaga aagcacgcgg tctgatgagt cgtttcatta ttgaaaatcg gctgaccaaa
     4741 ccagagcaac tgactggttt taatagcgaa ggttacttct ttgatgaaga ttcctccagc
     4801 aatggcgaac tggtgtttaa acgctacgag cagcgttaag tttgtagtca atgccggatg
     4861 cggcgtgaac gccttatccg gcctacaaaa gcatgcagat tcaatatatt gcagaattaa
     4921 cgtaggcctg ataagacgcg ccagcgtcgc atcaggcgct gaatgccgga ttgttacccg
     4981 gcatttgtca tttagcggtg atgtttgcct ggaccatgac cgccgtgatg atcatgagga
     5041 gctttcttat ggtggcgcgg cggtggcggc ggtccgtgtg ggtgccagcg attgcctcgc
     5101 cattcataat gttgtttcca ccagccgtgg tcgcgccagt gacctccatc ccagtaatag
     5161 ccacgattat cacgatcgcc tatctgtaat tttactgacg ggactaacgt aatttccgca
     5221 gcctgtgctg ccatgggagc gaccagaacc agggaaagtg cgagtacgat agattgcatc
     5281 tttttcacag gttactcctt ttatcattgc ccgttatggg tcgatatccg gagattacgc
     5341 gatgagaacg acggttgtta ttctccgcaa tcctaatctc taagttaccg catttattga
     5401 taatttctcc tttttccctg tcgtttttct ccataatttc ttcataaaaa agccgggctg
     5461 cataaaagca aacccggcct gattgagata atgaatagat tttactgatg attcatcatc
     5521 aatttacgca acgcagaaaa atcggcgggc agattatgtg aaagcaaggg taaatcagca
     5581 cgttctgcca gctcttttgg cagatccaac gtttcaccga gaatcgcttc cacgctctct
     5641 ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat attcgcctgg attcaactgg
     5701 tcacgcagcg cacgatacgc tacggcagcg tgcggctccg aggtgtagcc cagttctttt
     5761 aactcacgca ttgtctgttg cgtggtttca tcatccacgg ctgcataacc cagctctttc
     5821 agttgccaga ttttgcggcg gaacaactct tccacacgcg gccagttgtt cggctgactc
     5881 acgtccatcg cgttggataa cgtcgcctga gtcgctttgg gtgaccactg accgtcgtgc
     5941 aggaaacgtg gcacggtatc gttcacgttg gtcgcagcaa taaaacgttt caccggcaga
     6001 ccgaatgact tcgccagcag acccgccgtc aaatcgccga agtttccgct tggcaccgag
     6061 acaaccagct ggttgcgcgc ctcctgcggc agctgcgcaa cagcttcaaa gtagtagcaa
     6121 atctgcgcca gcaaacggct gatgttaatc gagttagccg agtttaaccc cagcgccact
     6181 ttcagttctt catcatcaaa cgcctgcttc accagcgcct gacaggcatc gaaatcgccg
     6241 tcgatggcaa cagtttcgat attgccgccc aacgtacaga acagtttttc ttgcagtgga
     6301 ctgattttgc ctcgtggata gaggataacc actttcacat tcggcaaacc gtagaaagca
     6361 tgagccactg cagctccggt atcaccggag gtcgcggtca gaatggtcac tggcttatca
     6421 tccgcaatat gggtcagcat ttgtgccata aagcgaccgc cgaaatcttt aaatgccagc
     6481 gttggcccgt ggaacaattc cagacaaccg acatcgcttt caacattggc gaccggagcc
     6541 gggaaggcaa acgccgcgcg cacgcgctct tccaggattt cctgtgggat ttcatcacca
     6601 ataaacgccg agaggatctt cgcactgcgg gtgacaaaat ccagcttcag catctcatca
     6661 atttcagtca ggctgaattc cggcaggtcg tgcggaaaaa acagcccctg atttttgccc
     6721 aacccctggg ttacggcttg cgcaaagctg acctgctcgt tgtgatcttt cagattgtag
     6781 agtttcattt agttttccag tactcgtgcg cccgccgtat ccagccggca aatatgaaca
     6841 aaaccttcct gattttgcag gtagttctta cccaaccagt cggcaacgcg ctgggcggta
     6901 tccggcttgt cacatagagc gaacaaggtc gggccggagc cggagatacc gctcactacc
     6961 gcgccgattt ccgcgaccgc ctgccgcgcc tgccggaagc caggcagtaa ccgttcacgg
     7021 tagggttcag cgataacatc tttcatcagc ttcgcggcaa gctcaggctg acgggaatag
     7081 caggcgtgaa tgaagccagc cagatgtcgc ccgtgcgcaa tgcaatcctg gcggcgatac
     7141 tgcgccggta aaatagcccg ggcttctgcc gtcgagactt taattcccgg atacgccagc
     7201 acccacagcc actcatcaaa ccctggcact tgctggctga tgatgtcgtt ttcttcgatc
     7261 atcaactgca taccaccaag aaaacacggt gccacgttgt cgtaatgaat gctgccggag
     7321 atacgtcctt ccagctcgcc catcaaagcc agcaaacgag tgtcattaag cggcttgccg
     7381 cagtgttcat tcatcgccat cagcgccgcg accaccgaac aggcgctgga gcctaagccc
     7441 gaaccgatcg gcatattctt ttccagagtc atcgccactg gaatttgctt gccaagctcc
     7501 tggcaaaaac gctcccagca ctgataaacg atattttccc gtggttctga cggcagctta
     7561 tcggcaaagc gtccgaggtt gttgagactg aatgtctctg ccgcctcaac cgtgactaca
     7621 tctccgagca atgcaccatc aacgggtgcc accgccgccc cgagcacatc aaacccgacg
     7681 ctcatattgg cactggaagc cggggcataa actttaacca tgtcagactc ctaacttcca
     7741 tgagagggta cgtagcagat cggcaaagac accggcagcg gtaacgtcat tgcccgcacc
     7801 atatccgcgc agcaccaacg gcagcggctg ataatagtgg ctataaaagg ccagggcgtt
     7861 ttcgccattt ttcactttga acagcggatc attcccatcc acttcggcaa tcttcacgcg
     7921 gcagacacca tcttcatcaa tattgccaac atagcgcaaa acttttcctt catcacgggc
     7981 cttcgccacg cgcgcggcaa agagatcgtc gagctgtgac agattcgcca taaaagcggc
     8041 aacatcaccc tcggcgttaa actctgcggg cagcacaggt tcaatttcaa tatccgccag
     8101 ctccagttca cgtcccgttt cacgagcgag aatcaatagt ttacgcgcca catccatacc
     8161 agaaagatca tctcgcgggt ccggttcggt ataacccatt tcccgcgcca gcgtggtcgc
     8221 ctcggagaaa ctcatgcctt cgtctaactt gccgaagata taagaaagcg aacctgaaag
     8281 aatgccggag aacttcacca attcatcacc tgcattgagc agattttgca ggttctcaat
     8341 aaccggtaat ccagccccaa cgttggtgtc atagaggaat ttacgccgcg atttttccgc
     8401 cgcataacgc aactgatgat agtaatccat cgacgaggtg ttggcctttt tgttcggcgt
     8461 gacaacgtgg aaaccttcgc gcaggaagtc ggcatattga tccgccactg cctggctgga
     8521 ggtgcagtca acaatgaccg ggttcagcag atgatattct ttcacgaggc gaattaagcg
     8581 cccgagatta aacggctctt tggcttgtgc aagttcttcc tgccagtttt ccagattaag
     8641 gccatgcaca ctagtgagca gagccttcga gttggcaacg ccgcagacac gtaagtcgat
     8701 atgtttattc ttcagccagc tttgctgacg cttcagttgc tccagcagcg caccgccaac
     8761 gccaccgacg ccaatcacaa acacttcgat aacctgatcg gtattgaaca gcatctgatg
     8821 agtaacgcgc acgccagtgg tcgcatcatc gttatttacc acgacagaga ttgagcgttc
     8881 agaagatccc tgagcaatgg cgacaatgtt gatattggcg cgggccagcg cggcaaagaa
     8941 tttcgccgag atcccacgca aggtgcgcat accatcacct accaccgaga taatggccag
     9001 ccgttccgtc accgccagcg gctccagtaa gccttctttc agttccaggt agaactcttc
     9061 ctgcattgcc cgttcagctc gcacacagtc gctttgtgga acgcagaaac tgatactgta
     9121 ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg gcgcgtgaca ttgcagcaaa
     9181 gacgcgcgcc gccatgccaa ccatcccttt catccccggg ccggaaacgc tgaacattgc
     9241 catgttattc agattggaaa tgcccttgac cggtaattcg tcttcatcac ggctggcacc
     9301 aatgagcgta cctggtgctt gaggatttcc ggtattttta atcaggcaag ggatctggaa
     9361 ctgggcgatg ggggtaatgg tgcgggggtg aagaacttta gcgccgaagt aggaaagctc
     9421 catcgcttcc tggtaggaca tcgacttcaa caacctcgca tcgggcacct gacgcgggtc
     9481 gcaggtatag accccgtcaa cgtccgtcca aatctcgcaa caatcggcgc gtaaacaggc
     9541 agccagcacc gccgcggagt agtcggaacc gttacgtcca agcaccacca gttcgccttt
     9601 ttcattaccg gcggtgaaac ctgccatcaa caccatgtga tcagccggaa tgcggcttgc
     9661 cgcaatacgg cgggtggact cagcaatatc gacggtagat tcgaggtaat gccccactgc
     9721 cagcagtttt tcgaccggat cgataacggt aacgttgtga ccacgcgctt ctaacacgcc
     9781 ggccataatg gcgatcgaca ttttctcgcc acggcaaatc agcgcagcgt tgatgctatc
     9841 cgggcactgc cccaacaaac taatgccatg caggacatgt tttatttggg caaattcctg
     9901 atcgacgaaa gttttcaatt gcgccagcgg gaaccccggc tgggcggcgg cgagtcccgt
     9961 caaaagttcg gcaaaaatac gttcggcatc gctgatattg ggtaaagcat cctggccgct
    10021 aatggttttt tcaatcatcg ccaccaggtg gttggtgatt ttggcggggg cagagaggac
    10081 ggtggccacc tgcccctgcc tggcattgct ttccagaata tcggcaacac gcagaaaacg
    10141 ttctgcattt gccactgatg taccgccgaa cttcaacact cgcatggttg ttacctcgtt
    10201 acctttggtc gaaaaaaaag cccgcactgt caggtgcggg cttttttctg tgtttcctgt
    10261 acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg tggtggtaat ggtggtgcta
    10321 atgcgtttca tggatgttgt gtactctgta atttttatct gtctgtgcgc tatgcctata
    10381 ttggttaaag tatttagtga cctaagtcaa taaaatttta atttactcac ggcaggtaac
    10441 cagttcagaa gctgctatca gacactcttt ttttaatcca cacagagaca tattgcccgt
    10501 tgcagtcaga atgaaaagct gaaaaatact tactaaggcg ttttttattt ggtgatattt
    10561 ttttcaatat catgcagcaa acggtgcaac attgccgtgt ctcgttgctc taaaagcccc
    10621 aggcgttgct gtaaccagtc gaccagtttt atgtcatctg ccactgccag agtcgtcagc
    10681 aatgccatga ctcgttcgcg taaagcttgc agttgatgtt ggtctgccgt tgtgtcactt
    10741 ttcgccggtt gttgtattaa tgttgctaat tgatagcaat agaccatcac cgcctgcccc
    10801 agattgagcg aaggataatc cgccaccatc ggcacaccag taagcacgtc agccaacgct
    10861 aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc
    10921 catgaagatt tttcctctaa cagcggcacc agttctactg gcgtggcgta gtaatgatat
    10981 ttcgcccgac tacgcgcagt ggtggcgaca gtgaaatcga catcgtgtaa cgattcagcc
    11041 agtgtcggga aaactttaat attatcaata atatcaccag atccatgtgc gacccagcgg
    11101 gtggctggct ccaggtgtgc ctgactatcg acaatccgca gttcgctaaa acccatcgtt
    11161 ttcattgccc gcgccgctgc cccaatattt tctgctctgg cgggtgcgac caggataatc
    11221 gttatacgca tattgccact cttcttgacc aaataaccgc gaacagggtg atcactgtca
    11281 acttattacg cggtgcgaat ttacaaattc ttaacgtaag ccgcagaaaa agccctttac
    11341 ttagcttaaa aaaggctaaa ctattttctg actgcactaa cggtttaatt gttaaaaaat
    11401 gctacatatc cttctgttta cttaggataa ttttataaaa aataaatctc gacaattgga
    11461 ttcaccacgt ttattagttg tatgatgcaa ctagttggat tattaaaata atgtgacgaa
    11521 agctagcatt tagatacgat gatttcatca aactgttaac gtgctacaat tgaacttgat
    11581 atatgtcaac gaagcgtagt tttattgggt gtccggcccc tcttagcctg ttatgttgct
    11641 gttaaaatgg ttaggatgac agccgttttt gacactgtcg agtcctgagg gaaagtaccc
    11701 acgaccaagc taatgatgtt gttgacgttg atggaaagtg catcaagaac gcaattacgt
    11761 actttagtca tgttacgccg atcatgttaa tttgcagcat gcatcaggca ggtcagggac
    11821 ttttgtactt cctgtttcga tttagttggc aatttaggta gcaaacatgc agaccccgca
    11881 cattcttatc gttgaagacg agttggtaac acgcaacacg ttgaaaagta ttttcgaagc
    11941 ggaaggctat gatgttttcg aagcgacaga tggcgcggaa atgcatcaga tcctctctga
    12001 atatgacatc aacctggtga tcatggatat caatctgccg ggtaagaacg gtcttctgtt
    12061 agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa
    12121 cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat gactacatca ccaaaccgtt
    12181 caacccgcgt gaactgacga ttcgtgcacg caacctgctg tcccgtacca tgaatctggg
    12241 tactgtcagc gaagaacgtc gtagcgttga aagctacaag ttcaatggtt gggaactgga
    12301 catcaacagc cgttcgttga tcggccctga tggcgagcag tacaagctgc cgcgcagcga
    12361 gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa attcagtctc gtgctgaact
    12421 gctgaagaaa atgaccggcc gtgagctgaa accgcacgac cgtactgtag acgtgacgat
    12481 ccgccgtatt cgtaaacatt tcgaatctac gccggatacg ccggaaatca tcgccaccat
    12541 tcacggtgaa ggttatcgct tctgcggtga tctggaagat taatcggctt taccaccgtc
    12601 aaaaaaacgg cgctttttag cgccgttttt atttttcaac cttatttcca gatacgtaat
    12661 tcatcgtccg ttgtaacttc tttactggct ttcattttcg gcagtgaaaa cgcataccag
    12721 tctatattac gagtcacaaa catcatgccg gccagcgcca ccaccagcac actggttccc
    12781 aacaacagcg cgctatcggc agagttgagc agtccccaca tcactccatc cagcaacaac
    12841 agcgcgaggg taaacaacat gctattgcgc cagcctttca ataccgcctg caaataaata
    12901 ccgttcatta acgccccaat cagactggcg attatccatg ccacggtaaa accgatatgt
    12961 tcagaaagcg ccagcaagag caaataaaac atcaccaatg aaagccccac cagcaaatat
    13021 tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa caaagaacgc cataaaagtc
    13081 agtgcaatca gcagaatggc gtacttagtc gcccggtccg ttaattggta ttgatcggct
    13141 ggcgtcgtca ctgcgacgct aaacgcgggg aagttttccc agccggtatc attgcctgaa
    13201 gcaaaacgct caccgagatt attagcaaac cagctgcttt gccattgcgc ctgaaaacct
    13261 gactcgctag cttcccgttt ggctggtaga aaatcaccta aaaaactggg atgcggccag
    13321 ttgctggtta aggtcatttc gctattacgc ccggcaggca ccacagaaag atcgccggta
    13381 ccgcttaaat tcagtgccat attcagctgc aggttctgct tacgccagtc cccttcgggt
    13441 aaagggatat gcacgccctg cccgccttgc tctaacccgg taccgggttc aatggtcagt
    13501 gccgttccgt taacttcagg cgctttcacc acaccaatac cacgcgcatc cccgacgcta
    13561 atcacaataa agggcttgcc taagatgata tttggcgcgt tgagctcgct aagacgcgaa
    13621 acatcgaaat cggcttttag cgttaaatca ctgtgccaga cctgaccggt ataaatccct
    13681 atcttgcgtt cttccacgtt ctgattgcca tcaaccatca atgactcagg taaccaaaaa
    13741 tggataaaac ttcgtttccg ctccacggtt ttatcctctt cctgcaccgt ataaagctcg
    13801 gtcacaggaa tagcgataag cggcccaacg agtttttgcg gcccgctggt actttggcga
    13861 atcgcatctt ccacatcgct acggtaatcg gcacgttcga caatcacttg ccgaatcagc
    13921 attatcggaa tcaacaacag caatactgca ccaaacaagg tggtcatttt ccagaacagg
    13981 ggggatttca acatagtctt ctcctttgca atggcagcag gatacgaagg ctatgtggga
    14041 agaatttgaa gctatgtgaa gtgacggtga agtcgtagcg aggccagcac gccaccttcc
    14101 tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg cgacctcact gacgaacgcc
    14161 aaccccagac cgctgctttt ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa
    14221 atacgtgaca gcgcgtagtc aggaatacca ctaccggtat ccagcacctt aagcgtgacg
    14281 tgttcctgat ccacttcggc gcttagcgtt atgcaaccgc tctcgggagt aaaatcgatg
    14341 gcgttatcca gtaaattccc cagcgcctga tccagtaacg ccggttcagc agcaacatta
    14401 acctcggtgg gcataacatg caaagtgatg tttttttctg ccaactgcac ggtgcgcgct
    14461 tcgctgacgc ggcgaaataa tgccgccaca tcaacagcag tcagaacgac ttcctgacga
    14521 ttctccagtc ttgcctggcg tagtaacgtt tccaccagcg cctgcattcg cgcattttgt
    14581 gtcagaatgt tgtcagtaaa acgagccact acttcaggcg gcggaccttc gcgtaaaatt
    14641 tccgccgcgc cacgaatcgc cgccagtggg ctttttagct catgggttaa cgcataaaca
    14701 tactgctcaa tatagttttt cccttccagc ttcacgcgca tactttccag cgcctgcgcg
    14761 agtttacgca gctcgctact accgagatcg ggaagagaaa cgggcttatt gtcagtgacg
    14821 gaatcagcat agcgagtgag ccgggcaatg gagcggttga tccaccaaac catgcctgcg
    14881 ccaatcacca gtgcaatccc caacaaaatg gcgctggccc ataaaattcg ccgctcgctg
    14941 cgtttaatga ccggagccat cgccgcgttc ggtttgccta cgctcaaaac gccaataagc
    15001 cgcgagccgc ccataatcgg cgcggcaaca tacatcaccg aactttcggg atcggcagga
    15061 ttttgcaacg tgctgcgcgc accatactga ccacgcaacg ttagccagac gtcattccag
    15121 cgcgaataat cctgtccaac ggctttattt gccgaatcga acaatacttt gccatgcgca
    15181 tcggtcatat agacatgata ttcattgcgc actttgttaa tgccaccaat attggcgcga
    15241 aacgggcgat gttgtagctg attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca
    15301 gagagcaaat cgggacgcgc cagctccgca agtaacgttg ccgtgtcgat caacgtcccc
    15361 tccgttgctc ttcgcacgcc cggtttaact tctttgacaa aaatagccag tacgaaccag
    15421 gccgccaccg ccaccagtaa aaaatagccc agcagcaacc gcatgccgat acgcattaca
    15481 agcccctcag gctatatccc atgccgcgat gagtattaat cggtgaaaga tcggggttga
    15541 tggcgcgcag cttggcacgc agcgttttaa tgtggatatc gacggtgcga tcgtaggtgt
    15601 cctgcgcatc ttcccacacg ctatccatta gttgctggcg ggaccagacg cggcctggcg
    15661 acttgagtaa cgtcttcagc aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa
    15721 accagctgat ctgcgccgcg ggttcattca attcaaaatg accaatacgg atgacgggag
    15781 acggcgtcga gaacttcttc acccgacgca gtaaggtgcg caccctggcg cacacttcgc
    15841 ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat ttccagccca agcaggcgat
    15901 cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa cgccggatgg agcgccagta
    15961 actggcggca caattcaaag ccactgatat caggcaaacc aacgtcgaga atcatgacgt
    16021 cgggaacctg ctggcgagct ttatccagca ccggcaaacc tcgctcaaag acctcgacgg
    16081 caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc tatcccttgc tcatcttcca
    16141 ctaaccagac cgtttcccgt tgcataaatc gcctctgtta ttgccgccac ggcatgacag
    16201 gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac tttgtcggag taagccagat
    16261 aagcgagcgc gttgcgtttg gcgtcataaa agcgcacgac ctgtaacgac ttaaagacca
    16321 gggacgtgcg ttttttgaat actacctcgc cctgagcttt gccgttttta atacgatccg
    16381 acagttcaat cggcccgact tgctgacaag aaatggccgc atcggaggta tcttctgcca
    16441 ggcccaatcc ccctttaata ccaccggttt tcgcccggct cacataacag gtgacatttt
    16501 tcacatcggg atcgtcaaag gcttccacaa caattttgtg atccgggccg atcattttaa
    16561 atacggtgtc gaccgaacca atctcttctg catgagccaa tggccccagc attatcaggc
    16621 ttaaagacag gatcaaatgc ttgtatttca tattgttacc atttttatct attacacgag
    16681 caattagttc gtcacggtaa ataacatatt gattaagaac aaaatctcaa ttcttttatt
    16741 tccgtcaagc cctaaaacat actctactaa gaaaaaaaca ctgaatgcta aaacagcaaa
    16801 aaatgctatt atccaattac ctgatgtcag gtgctcgttg ttgaaaggat gaggatattt
    16861 tatggatcag gccggcatta ttcgcgacct tttaatctgg ctggaaggtc atctggatca
    16921 gcccctgtcg ctcgacaatg tagcggcgaa agcaggttat tccaagtggc acttacagag
    16981 aatgtttaaa gatgtcactg gccatgctat tggcgcgtat attcgtgctc gtcgtttgtc
    17041 gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt ctggacatcg cgctgcaata
    17101 ccgcttcgac tctcaacaga catttacccg cgcattcaag aagcagtttg cccagactcc
    17161 tgcactttac cgccgttctc ctgaatggag cgcctttggt attcgcccgc cgctacgtct
    17221 gggtgaattc acaatgccag agcacaaatt tgtcaccctg gaagatacgc cgctgattgg
    17281 tgttacccag agctactcct gttcgctgga gcaaatctct gatttccgcc atgaaatgcg
    17341 ttatcagttc tggcacgatt ttctcggcaa cgcgccgacc attccgccgg tgctctacgg
    17401 cctgaatgaa acgcgtccga gtcaggataa agacgacgaa caagaggtat tctataccac
    17461 cgcgttagcc caggatcagg cagatggcta tgtactgacg gggcatccgg tgatgctgca
    17521 gggcggcgaa tatgtgatgt ttacctatga aggtctggga accggcgtgc aggagtttat
    17581 cctgacggta tacggaacgt gcatgccaat gctcaatctg acgcgccgta aaggtcagga
    17641 tattgagcga tactacccgg cagaagacgc caaagcggga gatcgcccaa ttaatctgcg
    17701 ctgtgaactg ctgattccta tccgccgtta acgctgcagc tcatctaatg caggggcgtc
    17761 cagatgcgag atgtcccctg cggtttcaac gacccagcca gacgccagcc acaggctttc
    17821 ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt aagcggcgct ctgcccatgc
    17881 tggtaatccg agaatcgtac tcaccagaca tcccagtgca ataccatggc tcaccagcag
    17941 cggtcggctt ccctgcggta agtcccggca agattccagt gccgcattga cgcgatcgct
    18001 gagctcctgc attgactcgc cttcaggaat acgcccgtca acggtgccat tgaccagctg
    18061 ccgacgccag ttctcttctt cttcggtcag agaatcgata tgtctttttt ccagcacacc
    18121 catgtttaat tcacgcaggc gagaatcaaa gatgatgtca cagccgcagg cctgggcgat
    18181 gatttccgcc gtacgccggg tgcgtcctaa atcgctactg ataatatgcg taatgccaag
    18241 ctctttggca cgggttgcca cctgcatcgc ctgttgctca cctttggcgg tcagcgggct
    18301 gtcagactgg ccctgaatac gtcgctcggc gttccactgc gtttcaccgt ggcggactag
    18361 gtatacctgt aacatgcttt ttttccgtat actgctgtaa cgttaatttt ctgagtaatg
    18421 ctgattatgc accaagttgt ctgtgcgacc accaatcccg ctaaaattca ggccattctg
    18481 caggcatttc acgagatctt cggcgaagga tcctgccata ttgcatccgt tgccgtcgag
    18541 agcggtgtac cggaacaacc ctttggcagt gaggaaacgc gcgctggcgc acgaaatcgg
    18601 gtagcaaatg cccgccgttt acttccagag gctgattttt gggtggcgat tgaagctggc
    18661 attgatggcg acagcacttt cagttgggtg gtgattgaaa acgccagcca gcgcggcgag
    18721 gcgcgttcgg caactttgcc gttacctgcg gtgattctgg aaaaagttcg tgaaggcgag
    18781 gcgcttggtc ccgtgagtcg cgttataccg gtattgatga gattggtcgt aaggaagggg
    18841 cgatcggcgt gtttaccgcc gggaaactca ctcgcgccag cgtctatcac caggcggtga
    18901 tcctggcgct gagtccgttt cacaatgccg tgtatcaggc attgtaggac ggataaggcg
    18961 ttcacgccgc atccgacatt cggtgcacga tgcctgatgc gacgctgccg cgtcttatca
    19021 ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactgacg cagctccacg
    19081 ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcccgc gccgagttca
    19141 ttttttaact cacgctgact catttcgcca cgcaacagct cttcgacaat acgcacgcga
    19201 gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga
    19261 tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt
    19321 tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg
    19381 gggaagcaaa atgactcccc cggttgtact cgctaaagag tacgatagca tatcataaac
    19441 gtgcggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca
    19501 tgaaatagcg gtagtaagca tcataagcca gcacgttctt cacataaccg cgcgtctcgg
    19561 agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg tggccaagcc
    19621 aggttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata cggttattgc
    19681 caaactgctg ataaacatat tgcaggtaac tggtgccaat gttgatgttc gtttccggat
    19741 ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc gtatgggtcg
    19801 ctgtaccagg cataatctgc atcaggccgc tggcccctac cggtgatttc actttcggat
    19861 tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tctttcccac
    19921 tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc
    19981 aaagcttccc ggcgatcgtt gcctgaacgc taagatccca ccattggtta ttgaaagcat
    20041 accgagccag ttgagcctgc tctgtttttg acttgctctt caccagattg gcccactcgc
    20101 tacgcgcggt attatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc
    20161 cctgagtcag ggtgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt
    20221 caccgatgcg ttgtgctgca accatcgggt agaaaccacg ctgttgtatg agttgatgca
    20281 aaatctcttt cgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac
    20341 gccattcatc tttctctttt gcttccatcg gcagacgcgc cagccaggta ttcaggccgc
    20401 gacgatcgcc ggtgccaagc gccattcgca cacggcgttc aatcaacgac gtggattgcg
    20461 agcgcataat ggcgtcatcg cgccatttcg cctgctcatc ggtgacatca ttgcccatca
    20521 aacgccaggc gacgatatcg cgcagctcct gaatctgatc ttcattaagc tgctgcgcct
    20581 gggcaagcga cgggatcatc agccgtgcat tttcggcatc ctgccgcgcc acactggcaa
    20641 acgccaccgc cgccatttga cgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca
    20701 gtaccgtatt agggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg
    20761 caggcatctg ccctgccagc actgttacca ggcctgtgtt acccgctttc atcgccagac
    20821 ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata
    20881 acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc
    20941 aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat tgcgcttcgg
    21001 tagttccggg cttttccggg ctaaaggcta acaagccacg ccagtcttca cgccgcgcca
    21061 gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa
    21121 caaagttagt gaccgtcacc gccggttgat tcatcaggtc atcggtgatc tggcggtatt
    21181 ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca
    21241 tctgtcgatt atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg
    21301 agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc
    21361 aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag
    21421 cgtcatgccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga
    21481 cactttttta cttttactgc gaaggtgatc gaccaactgg gattaggcgg ggaaacaggc
    21541 tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt ccaacgtggc
    21601 tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt
    21661 gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg
    21721 cgcgggtaag tccaccctgc tgcgcattat ggcgggcatt gataaagaca tcgaaggtga
    21781 agcgcgtccg cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc
    21841 ggaacacacc gtgcgtgagt ccattgaaga agcggtttca gaagtggtta acgccctgaa
    21901 acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc
    21961 cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata acctgaacgt
    22021 acagctggag cgtgcggcgg atgcgctgcg tctgccagat tgggacgcga aaatcgctaa
    22081 cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga
    22141 catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cgtggctgga
    22201 acgcttcctg cacgacttcg aaggcaccgt ggtggcgatt actcacgacc gttacttcct
    22261 cgataacgtt gcaggctgga tcctcgaact ggaccgcggt gaaggtattc cgtgggaagg
    22321 taactactcc tcctggctgg agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga
    22381 agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg
    22441 ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca gcaccgaata
    22501 ccagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa
    22561 agtgctggaa gtcagcaacc tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct
    22621 gagcttctcg atcccgaaag gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa
    22681 atcgaccctg ttccgtatga tctccggtca ggaacagccg gacagcggca ccatcacttt
    22741 gggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg ataacagcaa
    22801 aaccgtttgg gaagaagttt ccggcgggct ggatattatg aagatcggca acaccgagat
    22861 gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgtgt
    22921 tggcgaactt tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg
    22981 cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc
    23041 gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc acgaccgttg
    23101 gttcctcgac cgtatcgcca cgcacatcct ggactaccag gatgaaggta aagttgagtt
    23161 cttcgaaggt aactttactg agtacgaaga gtacaaaaaa cgcacgctgg gcgcagacgc
    23221 gctggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat
    23281 gcggcgcgaa cgccttatcc ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt
    23341 aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatgcgg
    23401 cgtgaacacc ttttccggtc tacaacagcc ttagcatcct caatttcgtg gcgggtcacc
    23461 cgtgcaggtt ttttcaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac
    23521 tcatcaaaaa aactcatttt atacctcgcc tttgagcagt ttcgtcacgg ttatccttgc
    23581 tcccccatca tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcgtcc
    23641 tcttcaaccc gcacgaattc gatattgttc tcttccagca tctccaccag caagttctgg
    23701 aactctttgc gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg
    23761 ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcagcgc ctgcacgaac
    23821 ggatgctcac gcccttcgta ctttttgcag aacgcctggg tagtgacaaa atcggtgtca
    23881 ataaacgcca ctttattggc gtatttcact gcaaaatcaa tgtattgtgc gtggcccaac
    23941 gcgattttat cgtagtcgga atactgcaat gcgatctcat caccgccgag gtgtgaaaag
    24001 acataatcgc ggccatattc ccacgcactg gtggtgttga agatattggc aagtttgttt
    24061 accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac
    24121 ggcttcactt cggtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca
    24181 ccgctgatac tcataaaggt acgtttcgga tcgaccagca ccgtctcgat ccccagatgt
    24241 tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg ctggatcccc
    24301 ttttcagcca taaacttttt gatgccgttg ctccacacat cccagccatg cggatacggc
    24361 tccatgccct cttcgttgaa agcatgaata cggatatttt tctgatattt aaaggtttgc
    24421 aatagccagc gcagacgatc cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac
    24481 aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta
    24541 caggcgcgct ggataaggta gatatgtccg gtatgcagcg ggtagaactt accgaatacg
    24601 acaccaatgg ttttcttctg ccggggaaat tcaagcccca aaaaacggtg caatgcctcc
    24661 agcttttgcg cgctggggct tttaattttg gcattcagta actggcttaa ataccctttg
    24721 gtcataccgc tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt
    24781 ttcaggtaat caaatgacga catatctccc tccgtatctc tcattataag tcgtcgaaca
    24841 cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt
    24901 tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaaa
    24961 tccgctcctg gccactgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca
    25021 ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg
    25081 ctaagtcggc gctggtttcg gttaccttca cgccgccgac cacgttcaca aacacatcct
    25141 gatcggccat ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt
    25201 tttgttccag ccccactgcc acgcggcgcg gattcgccat tatcgagtga tcgaccagcg
    25261 cctgaatctc caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg
    25321 aggtcacttc atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc
    25381 cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc
    25441 tgcgtaaggt gcgaaaacgg gagtcggcat cgccatccag aagcaccgaa cagtcgatac
    25501 agtgttccag cactttcggg ccagccagcg aaccatcttt ggttacgtgt ccaaccatga
    25561 caatcgccac accgcgcgtt ttggcgaaac gcgtcagata agccgccgtt tcacgcacct
    25621 gcgccacgct accaggcgat gattgtacat ccgccatatg catcacctgg atcgagtcaa
    25681 ttaccatcag cttcggttgc tcttcttcgg caatcaggca gatctgctcg atgctggttt
    25741 ccgacaacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct
    25801 gttgcagcga ctcttcgccg gtgacataca gcgttttcat ctgctgggcc agtttgcaca
    25861 gcgtttgcag caacagcgtg gatttccccg caccagggtt accgccaatc agaatggcac
    25921 ttcctggcac cacgccgcct cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac
    25981 gcggcagctc ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac
    26041 cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg cgacgcagca agacgcacct
    26101 cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg
    26161 ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta
    26221 atgctgtgcc gcccgcagga tgacgggcag gcaattactt ctgattcagg ctgccagaga
    26281 ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca
    26341 cttttggctt ggcatggtag gcaatcccca gccctgctgc tttgatcatc ggcaggtcat
    26401 tagctccatc gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc
    26461 gagtcagagt tttcgctttg tactgcgcgt ctacgatgtc gccgatcaca ttgccggtaa
    26521 atttaccgtc catgatctcc agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc
    26581 gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg
    26641 tttccagctt gagcaccagc tgcgttaagc ctggcatcag cggcagattt tcacgcacct
    26701 gttgcagaat attggcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg
    26761 taaaatcgag ttcgccgcgc atcgcccgtt cggttacctc cgccaccatc tcgcccgttc
    26821 cggccagctt ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca
    26881 tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc cagcggggcg acatccagct
    26941 gcgcttcgtg ggccaggcgt gtagcccgtg cggtgagtga acctgccaga cgaatcacct
    27001 gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt
    27061 attgggtcag acgctgttta tccagcccac gaccatacag cagccagccg ctacgacctg
    27121 cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata
    27181 aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat
    27241 cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat
    27301 taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctaca
    27361 tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc agggggcgtc
    27421 atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac
    27481 cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact caccggatga
    27541 aaaacgcatt caggcgatcc tcgatcagtt aacagatgaa agccgtatcc tcgacgcggg
    27601 tgtgtatgac gaacaaggcg atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga
    27661 tcggctggcg ctcgacggta aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc
    27721 aattgcgggt aaaaacggac cgctcggcta tctgcgcctg acactcgaca cccatacgct
    27781 cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct
    27841 ctcactggca atcggcgtag tgctgacccg cacgctgcta cagggtaaac gcacccgctg
    27901 gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga
    27961 gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct
    28021 catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta tttttcgcca
    28081 aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtaattgg
    28141 tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg
    28201 cctggcgcgg cgcagtggcg gtggcggcgc ggtgttccac gatctcggca atacctgctt
    28261 tacctttatg gctggcaagc cggagtacga taaaactatc tccacgtcga ttgtgctcaa
    28321 tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa
    28381 aaccgtcgaa ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca aagatcgcgg
    28441 cttccaccac ggcaccttgc tactcaatgc cgacctcagc cgcctggcaa actatctcaa
    28501 tccggataaa aagaaactgg cggcgaaagg cattacttca gtgcgttccc gcgtgaccaa
    28561 cctcaccgag ctgctgccgg ggctcaccat gagcaggttt gcgaggccat aaccgaggcc
    28621 tttttcgccc attatggcga gcacgtggaa gcggaaatca tctccccgga caaaacgcca
    28681 gacttgccaa acttcgccga aacctttgcc cgtcagagta gctgagagtg gaacttcggt
    28741 caggctccgg cattctcgca tctgctggat gaacgcttta gctggggcgg cgtggaactg
    28801 catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tatttaccga cagcctcaac
    28861 cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gcctgtaccg cgcggatatg
    28921 ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg accaggaaaa agagctacgg
    28981 gagttgtcga cgtggatagc gggggcggta aggtaactcg ctgattaaaa tatccaagga
    29041 aggaaatatg atcgatctgg aaaaccagga acgggagatt atcaatttaa tgttcagcca
    29101 gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg ctgaagtaag
    29161 taaaatgtta ggtatctcga ttaattctct aaaacagatc gaaaagacgg aacggcttag
    29221 ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt tgctgatatg
    29281 cccttcctgg atgacggctg aacataaatg aatagattcg tgaatagata cttgaatgtt
    29341 ttttattttc tatccatatg aaaaataata gtaaatctat aaatcagttg ctattgatga
    29401 atagatacta aaaagccgga gcagtccccc actccggctt aacacgacaa tcgcctttca
    29461 gcgaaacaca attactcttt atcgcccagc agaacggatt ccagtgcgat tttgatcatg
    29521 tcgttgaagg tggtctgacg ctcagcggca gtggtctgct cgtgagtgcg gatgtggtca
    29581 gacacggtgc agatggtcag ggctttcgcg ccaaactctg cagccacgcc gtagatacca
    29641 gccgcttcca tttccacgcc gaggatgccg tatttttcca tcacgtcgaa catttcgccg
    29701 tccggagagt agaacaggtc agcggagaac aggttaccca cgcgagcatc aacgcccagt
    29761 gctttagctg catctactgc gttacgcacc atgtcaaagt cagcgatagc ggcaaagtca
    29821 tggtctttaa aacggatgcg gttaactttg gaatcggtgc aggcccccat accgataacg
    29881 acgtcgcgca gttttacgtg cggcagaact gcgccacagg aaccaacgcg gataattttc
    29941 ttcacgccga aatcggtgat cagttctttg gtgtagatgg agcaggacgg gatacccata
    30001 ccgtgaccca ttacggaaat tttgcggcct ttgtaagtac cagtgaagcc cagcataccg
    30061 cgaacgttgt tcacttcacg ggcatcttca aggaaagttt cagcaatata cttcgcacgc
    30121 agcgggtcgc ctggcatcaa aactacgtca gcgaaatcgc ccatttctgc attaatgtgt
    30181 ggggtagcca ttgttttatc ctttttgtga cataacaaag gcggagtcaa cgctccgccc
    30241 aaatccatca gaacatggct ttgccatatt ccatatcaga agtaccaaaa tattttgcca
    30301 gagtctggcc gatatccgcg aaggtttcac ggtgacccag tgagcccggt tttactttcg
    30361 ggccatatac cagtaccgga atgtgttcac gcgtgtggtc agtaccggtc caggtcggat
    30421 cgcaaccgtg gtcagcggtg aggatcagga tgtcgtcatc gcgcagcaga gacatcagct
    30481 ccggcagacg gcggtcgaac agttccagac ccgcagcata accggcgacg tcgcggcggt
    30541 ggccccagga agagtcgaag tcaacgaagt tggtgaagac gatggtgtta tcacccgctt
    30601 ctttcatctc tttgatggtg gcgtcaaaca gcgcgtccaa gccagtcgct ttcacttttt
    30661 tggtgatacc gcagttggcg tagatgtctg caattttacc gacagaaacc acctggccgt
    30721 gtttttcatc aaccagtttc tgcagcacgg tcggtgctgg cggctcaaca gccaagtcgt
    30781 gacggttacc ggtacgctgg aagttaccag ctttgtcacc gataaacgga cgagcgataa
    30841 cacgaccgat attgtagccg ccgttggtca gctcttcacg ggcgatttcg cacagttcgt
    30901 agagtttatc cagaccgaaa gtttcttcat ggcaggcaat ctggaacacg gagtcagcgg
    30961 aggtatagaa aatcggcttg ccggttttca tgtgctcttc gcccagttga tccagaatga
    31021 ccgtaccgga agagtggcag ttaccgaggt aacccggcag attagcgcgt tcgaccagtt
    31081 tatccagcag ctcttgcggg aagctgtttt cgtgatcgga gaaatatccc cactcaaaca
    31141 gaaccgggac accggcgatt tcccagtgac cagacggggt atctttaccg gatgacattt
    31201 cgtgcgccca tgcgtacgcg ccgataactt cagcgttgcc gtccattccc gccggaatga
    31261 aaccggtaga accttcgtgt gctttcgcca gccccagacg ggtcagattt ggcagattga
    31321 gcggcccttt acgaccatta tcagcttcgc ctttggcaca agcttctgcg atatgaccca
    31381 gggtgtcagc cccgacgtca ccaaagcgtt ctgcatcttc tgtagcgccg atgccgaatg
    31441 agtccagcac cataataaat gcacgtttca tatgttctcc gtactcagtg cttcaaattt
    31501 aaatgatcag atcagtatac cgttattcgc tgatacggcg atagacagtt ggtgtgcttt
    31561 ccggtgcgtt atcggcaagt ttaattgccg ctttcaccgc tttcgccgct tcctgccagc
    31621 tgttttcgtc tttcgcgagg ataaccgcca gcggacgctg accgtctacc tggtcgccca
    31681 gacgcgccat atcagtaaag ccgacgctgt aatcgatggt gtcagatgcc tgacgacgac
    31741 cgccgcccat tgcaaccact gccatcccca gcgcgcgggt atccatttca ctgacaaaac
    31801 cttcggtatc agcatagact gctttcgtca gcatcgctgt cggcagatac ttcgcgtagt
    31861 tctcaacgaa gtcggtcggg cctttttgtg ccgctaccat acgaccaaag acttctgccg
    31921 ctttaccgtt gtccagcacc gcctgcaatt tcgcgcgcgc ttcggcgtca tctttcgcca
    31981 gtttgccgga gatcagcatc tccacgcaca gcgccatcgt gacatcaaac agacgcgggt
    32041 tacgatactc acccgtcagg aactgcaccg cttcacgaac ttcaaccgcg ttacctgcac
    32101 tggaggccag tacctgattc atgtcggtga gcagcgcggt ggtgcgcacg ccagcgccgt
    32161 tagccacgcc aacaatcgct tcggcaaggg cttcagagag ttcgtaggtc ggcataaacg
    32221 cgccgctacc cactttcacg tccatcacca gcgcgtccag accttccgcc agtttcttcg
    32281 ccaggatcga ggcagtgatc agcgggatgg agtccacggt tgcggtaata tcacgggtcg
    32341 cgtagaaacg tttatcagcc ggagccagtg aactggtctg accgataatc gccacgccga
    32401 cgtctttaat aatttcgcgg aaacggttgt catccgggaa aatgtcgaag ccagggatgg
    32461 attccagttt gtcgagcgta ccgccagtat gaccgaggcc gcgaccggag atcatcggaa
    32521 tatagccgcc gcaggctgcg accatcggcc ccaacatcag cgaagtgaca tcgccgacgc
    32581 cgccggtcga gtgtttatca acaatcgggc cattcagatg caggcttttc cagtcgagaa
    32641 ctgttcctga atctcgcatc gccatggtca gcgagacacg ctcaggcatt gtcatatcgt
    32701 ggaagaaaat ggtcatcgcg agggcggcaa tctgcccttc ggaaatagtg ttgtcgcgaa
    32761 taccgttgat aaagaaacga atttcttcat cgctcagcgc atgaccatca cgttttttac
    32821 gaataatttc ttgtgcgaga aacaaggtaa cctcccgagg aaaagagtat gaggcggctt
    32881 ttgccggatg cgtctacgcc ttatccggcc tacgggtaga tacgtctcgt aggcctgata
    32941 agcgcagcgc atcaggcgta aagcatctta cttagtagct gctggcgctc ttaccgtcgc
    33001 cgtggcccag cgctttcagc aggcttgcca gcaggctgga agcaccaaag cggtagtgac
    33061 gcgcatcagc ccagtcagca ccgaacagtt catctgcgat ggcgagatat ttctgcgcat
    33121 cttccgcagt acgcacgccg cccgccggtt tgaaaccaac ggttttttct acgcccatat
    33181 cacggatcac ttccatcatg atgcgagcgc tttccggcgt cgcgttcaca gccactttac
    33241 cggtagaggt tttgatgaag tccgcacccg ctttgatgga gatttcagac gctttacgga
    33301 tcagcgcttc gtctttcagt tcgccggttt cgatgatcac tttcagcagt acattcgctg
    33361 ccgcgcaagc ctctttacag gctttcacca ggtcaaaacc aacctgctcg tcacccgcca
    33421 tcagcgcgcg gtacgggaac accacgtcaa cttcatcagc accgtaggcg attgccgcac
    33481 gggtttctgc cagcgcgatt tcgatgtcgt cgttaccgtg tgggaagttg gttaccgtag
    33541 cgatacggat ttccggggtg ccctgctctt tcagagtttt gcgagcaatc gggataaaac
    33601 gaggatagat acagatagcg gcggtattgc cgaccggagt tttggcctga tgacacagag
    33661 caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt caggtccatc aatttcagtg
    33721 cacgcaggct gcttgctttc agatcagtca tttcattctc ctggcttgtc gccaataaaa
    33781 ttcaccttgc gagtttgtta gtattctaac atctactccg caacacactt cgatacacat
    33841 cacaattaag gaaatctact tacaagtttg catcactgta atgcgatctg gttcaaataa
    33901 ttcactttca aatgaatgcg tcagtggtgg caaacgcatc aggatcaaag tgaacatcac
    33961 gaaacttctt acaatggcgc atcaccttcg gcataaaagg aaaagaagat gcccacttct
    34021 catgaaaatg cactgcaaca acgttgccag caaattgtca ccagcccagt gcttagcccg
    34081 gagcagaagc gccattttct ggcactggaa gcagaaaaca atctgcctta cccacagctt
    34141 cctgccgaag cccgccgcgc gctggatgaa ggtgtaatct gcgatatgtt tgaaggtcat
    34201 gcgccgtaca aaccgcgcta tgtcttaccc gattacgctc gttttctggc gaacggttcc
    34261 gaatggctgg agctggaagg cgcgaaagat cttgatgacg cactctctct gctgaccatt
    34321 ctttaccacc acgtaccgtc ggtcacatcg atgccggtct acctggggca actggatgcg
    34381 ttgttgcaac cgtatgttag aattctaaca caaaacgaga tcgatgttcg aataaaacgt
    34441 ttctggcgtt acctcgacag aaccctgcca gatgccttta tgcacgccaa tatcggcccg
    34501 tctgattcgc ccattacccg cgcgatctta cgtgcagacg cgcaggtttc accgaacctg
    34561 acctttatct acgatcctga aatcacccct gatgacctgc tgctggaagt ggcgaagaac
    34621 atctgtgaat gtagcaaacc gcacatcgcc aacggtccgg tgcatgataa aattttcaca
    34681 aaagggggct acgggattgt gagctgttac aactcactgc cgctggcggg tggtggcagc
    34741 acgctggtac gccttaacct gaaagccatt gccgagcgca gcgaatcgct ggatgacttc
    34801 tttacgcgca ctctaccgca ctactgccag cagcagatcg ccatcatcga tgcgcggtgt
    34861 gaattcctct atcaacaatc acacttcttt gagaatagct tcctggtgaa agaagggctg
    34921 attaaccctg aacgttttgt gccaatgttt ggcatgtatg ggctggcgga agcggttaac
    34981 ttgctgtgtg agaaagaagg gattgccgcg cgctacggta aagaagccgc cgcaaatgaa
    35041 gtgggttatc gcatcagcgc gcaactggcg gagtttgtcg ccaatacccc cgtgaaatat
    35101 ggctggcaaa aacgcgccat gttacacgca cagtcgggga tcagttccga tatcggcacc
    35161 acgccgggcg cgcgtttgcc gtatggcgat gagccagatc cgatcaccca tctgcaaact
    35221 gtcgccccgc atcatgctta ttattattcc ggcatcagcg acattctgac gctcgacgaa
    35281 accatcaaac gtaacccgca ggcactggta cagctttgcc tcggtgcctt taaagccgga
    35341 atgcgtgaat ttaccgccaa tgtcagcggt aacgatctgg ttcgcgttac cggttatatg
    35401 gtgcgtttgt cggatttaga aaaatatcgc gccgaaggtt cacgcaccaa caccacctgg
    35461 ctgggcgaag aagccgcacg caacactcgt attctggaac gccagccgcg cgtgataagc
    35521 catgaacagc agatgcgctt tagtcagtaa gattatcccc ttctcctgcg ttgacgggcc
    35581 aggcagtcgt ctggctctgt ttttgcaggg ctgcaatctg cgctgcaaaa actgtcacaa
    35641 tccgtggacg atgggacgtt gcaatgactg tggggagtgc gtgccacagt gtccgcatca
    35701 ggcgttgcag attgttgacg gcaaagtggt gtggaacgct gtggtctgcg agcaatgtga
    35761 tacctgcctg aagatgtgtc cgcaacatgc cacaccaatg gcgcaatcca tgagcgtgga
    35821 cgaagtgctt agccatgtgc gcaaagcggt gctgtttatt gaagggataa cggtaagcgg
    35881 cggcgaagcc acgacccaac tgccgtttgt agtggcgctg tttactgcta tcaaaaacga
    35941 tccgcaactg cgccatctca cctgtctggt ggacagtaac ggtatgttga gcgaaaccgg
    36001 ctgggaaaaa ttactcccgg tgtgtgacgg cgcaatgctc gatctcaaag catggggtag
    36061 cgaatgtcat caacaattaa ccggacgcga taatcaacag attaagcgca gcatctattt
    36121 gctggcagag cgcggcaagc tggcggaact gcgtttgctg atgatcccag gccaggtgga
    36181 ttatttgcaa cacatcgaag aactggcggc gtttatcaag ggacttggtg atgttccggt
    36241 tcgcctgaac gcgtttcatg cccacggcgt gtatggcgag gcgcaaagct gggcgagcgc
    36301 cacgccggaa gacgttgagc cgttggctga tgcattaaag gtgcgcgggg tgagccggtt
    36361 gatatttccg gcgctctatt tgtgacatgc cggatacggc ctacggttcg gcacagactt
    36421 gtaggcctga taagacgcgt caagcgtcgc atcaggcatt gtgcgccaac tgccggatgc
    36481 ggcgtgaacg ccttatccgg cctacggttc ggcacaaacc tgtaggcatg ataagacgcg
    36541 ttaagcgtcg catcaggcat tatgcgccga ctgccggatg ctgcctacga agggcaaact
    36601 acccacgaac gttaaacacc gcatacgtgt tattaagcaa cacttcggca atctcatccg
    36661 cgggttccgg gcgtaactcg caaagcacgg caaacacacg ggcagcctgt tccgggcggt
    36721 taggctgccc ctgaaaaccg ttgagcggca tatccggcgc atcggtttcc agcaacaacg
    36781 atgccagcgg taattttgcg atgacatcgc gggttttact ggcgcgtgga taggtgatag
    36841 taccgcctac gccgattttg tagcccagct gtacaaaccg ttcagcctgt tgctggctgc
    36901 cagaaaatcc gtggacaaca ccagcacgcg gcaaatcgtg gtgtttaaga tgtatcgcca
    36961 gtttgtcgtg cgtgcgccgt gaatgcagga tcaccggcag atcgtagcgt ttcgccagtt
    37021 tcagttgttc gtcgagtaac cactgctgcc tctcaaattg cggatcgtcg ccgaagagat
    37081 ccagaccgat ctcccccacc gccaccacct tcgccggacg cctttccagc gcctgctgta
    37141 gctgctcaag agacacatcg ctatgttttt ccaacatacc aggatgcaag cccaatgcgg
    37201 catacagcgg ttgataattt tccgctaacg cctgcacgcg ggcaaaattt tccgcctctg
    37261 tcgccggaac aatgatcttg cctacgcccg cttgtgccgc gcgttgcagg ctggcctctt
    37321 catcgccaat aaaaggcggg aaatcaaaat ggcagtgagt gtcgataaaa cggcaaatca
    37381 agccagatcc tcattgttaa atgtcgggtc gttcgcctgc ggagcgtcgc tcacttcggc
    37441 aaccagcgta tcgttggcga ctggcgcagg cggaatgaca atcgattccg gcgtcaccac
    37501 tggcaccaga tggcgggtaa gcggcgcttt ttcagttaat agcttgccaa ccgtggcgag
    37561 gaaataacga ccgcataaac gcccaagttt atagtcttca cgcagcgccg gaatccgact
    37621 gccaagcgcg atactatgta atggcttcgg cggataaatt tcggatatcc gcagcttgcc
    37681 cggtggtttc tcaataaatt gctgaatgtc acgatagctg gtttcatgat gctgcaccag
    37741 attgaccagc ggctgcaggc tactgtcacc cagccagcgt tccatgcgtt tgaaccactg
    37801 cggcgtgtag tacatttgtg acggcacagt gcggatgacg accaacgttt tagcgccctg
    37861 ccttgccgcc tctttaaccg gaatcgcatc actgatcccg ccatccaggt agttaatgcc
    37921 ttccagcgac actccgctac gataaaagcc aggtatcgca ctggaggcgc gaatcacatc
    37981 cagccagttt tgtttggttg gtaaaaagta attcggcgcg tagtcatcct gacgacaggc
    38041 gcacatataa aacgatttgc cgctgtcaaa caaccgcgcg gcggtatcca tttgcaacgg
    38101 catctggctt gcggtggcct ccaccagcca gtcgagatcg ataagatttc ctccacggac
    38161 aaagcgcaat ggatcgaaaa attcgcgttt tgtggtatag cgcatgatga ctttgcgcgc
    38221 gtaaccgggc tgattgcaga taaacgccga gaggttctgc gccccggcag atgtgccgag
    38281 ataaagatcg aaaggattaa actgcgcgcg cataaactca tccagcacgc cagccgtgaa
    38341 aattccacgc tgtccgccgc cttcgcacac cagagctatt cgtccaggct ggaacggcct
    38401 tagcgacaac ggcgcaatat taccaagcgt tacaggtatt cgctgcccca cttctgcctt
    38461 cctgtttttg ttgtgaaaga aagacaaagt aacgcaattt attattcgct aaaaccgcaa
    38521 aagccagtcc gctggactgg ctttggataa aattttctta atggtctgaa atctagggtc
    38581 gttttcggcc catgaacaaa ctcactagga acagaataat cccgacgaca aagacaattt
    38641 tagctgcgcc tgcagcggta ccggccagac caccaaaccc aagtgcggcg gcgattaacg
    38701 cgataaccag aaatatgatg ccccaacgaa acataagcgt ctcctttacc atagttaatg
    38761 tcaccgcttg atatgagcgt gaaaatcact caatgcgata tttttagtgt ggtgcacatc
    38821 gcgcctcccg acaaagttcg ggaggacgaa ttacttagtt ttcagatcat ttttaacgct
    38881 tttcacacca tctaccgctt tggcgatact ttcagcacgg tcactttgtg cctgagaatc
    38941 tacggtaccg gagagctgaa ccacgccatc ggtggtttca actttcactt tacgtgaagg
    39001 gacgatatcg tccgccagca gtttggcttt gatttcactg gtggtggcgg tgtcacccgc
    39061 gtagcccttc accgagcctt ctttagcgtc gcgaacgtgc agtttgtcgc tgacagaggt
    39121 cacgccttca acgcctttcg ccactttcac tgcctcttca gcctgggcct gactttcaac
    39181 gaaaccgctc agggtcacga ctttttgatc ggttttgaca gagatatcgg tgctcttgat
    39241 gttgtcatga tccaccagcg ccgccttcac tttcgcggtg atggcgctgt catccatgaa
    39301 attaccgact ttattcatag agctatcgac tttttgccct gcgctttcat tggtagtctg
    39361 cgcgttgttt tccgcgtagg cagagccggt cgcgacggca gaggtcaaca ttacagccag
    39421 cagagttttc gaaatcttca gtcttgtcat agtcatcgat ttattcctgt atgtttgctc
    39481 gtaatttgag cctagcaaca cgaggttgca ttgctgaata gggagagact tcaccctcta
    39541 cagaagtcaa tggtcgccat cacaaaagcg atgggtgatg aataacgacc attacagcct
    39601 ctgaatcagt tattaatatc ggtagaatga caatcgacgg ctttagatac tgatatctac
    39661 gcattgaacg gtatttaacg ccatcagaaa tgtcatcact ttgttaaata tagatcacaa
    39721 ttttgaaacc gctcgggata tcagcgaaaa cataagcaaa agtgaatgtt ttaagaacat
    39781 tccgtaagcg gctaataagg aagggaaatt gacagggcgc agcggctacc gcgccctgga
    39841 ggcaagaatt aatgctcgcg ggtctggtgg aactgaacgt ccggataacg ttcctgtgcc
    39901 aggcgcaggt tgaccatgct ggtagcgatg taagcgaggt tatcgccgcc atcaagcgcc
    39961 agttggcttt cgttcttacg cttgaactct tcgaatttct tcgcgtctgc acattctacc
    40021 cagcgggcag tggcaacgtt gactgactca tacactgctt caacgttgta ttcgctcttc
    40081 aggcgcgcta ccaccacatc aaactgcagc acaccaaccg caccaacgat cagatcgttg
    40141 ttggagattg gacggaacac ctgcaccgcg ccctcttcgg aaagctgtac cagccctttg
    40201 agcagctgtt tttgcttcag cggatctttc aggcggatac gacggaacag ttctggtgcg
    40261 aagttcggaa taccggtgaa cttcatcatc tcaccctggg taaaggtgtc gccgatctga
    40321 atggtgccgt ggttgtgcag gccgaggata tcgcccggat acgcttcttc aacgtgcgaa
    40381 cggtcacccg ccataaaggt cagcgcgtcg gagatcacca catctttcgc agtgcgcacc
    40441 tggcgcagtt tcatgccttt ttcatattta ccggacacca cacgcataaa cgccacgcgg
    40501 tcgcggtgtt tcggatccat gttggcctga attttaaata cgaagccggt aaatttatct
    40561 tcgctcgctt ctacggtacg ggtatcggtc tgacgcggca tcggcgcagg cgcccactcc
    40621 accaggccat tcagcatatg atcaacgccg aagttaccca gcgcagtacc gaagaatacc
    40681 ggagtgattt cgcccgcaag gaacagctct ttgtcgaact cgttagacgc gcctttaacc
    40741 agttccagtt cgtcacgcag ctgctgtgcc agatcttcac caaccgcagc atcgagatcc
    40801 gggttattca gccctttaac aatgcggact tcctgaatgg tgtgcccttt accgctctga
    40861 tagagatagg tttcatcttt ataaaggtgg taaacgcctt taaacagctt gccgcagcca
    40921 attggccaag tgatcggtgc gcagccaatt ttcagctcgt tctcaacttc atcgagcagt
    40981 tccatcgggt cgcggatatc acggtcaagt ttgttcataa aggtgaggat cggcgtgtcg
    41041 cgcagacggg taacttccat cagcttacgg gtacgatctt caacaccttt tgcggcgtcg
    41101 ataaccatca ggcagcagtc caccgccgtc agggtacgat aggtatcttc cgagaagtct
    41161 tcgtgccccg gggtgtcgag caggttaaca aggcaatcgt gatacggaaa ctgcatcaca
    41221 gacgtagtaa tggagatccc acgctgcttt tccatctcca tccagtccga cttagcgtgc
    41281 tggttggaac cacggccttt tactgtaccg gcggtctgaa tggcctgtcc gaacagcagc
    41341 accttctcgg tgatggtagt tttaccggcg tccgggtgag aaataatggc aaaagtgcgg
    41401 cgcttcgcca cctctttcaa ataaggagac aacgtcataa ttctttcttc ttgagtaagc
    41461 ggcatcgcgc cgcgcttgtt gaaaacgaaa aattgcggct attttaccca tcaacggggg
    41521 ggaggcaatc agtgtttaca caggagctgc tccagttcgt gcaacgaaga aacggtccag
    41581 gtgggcgcga tgccttctgg ttgctcgcga ttgtgcgcat tcagccagca ggtcgcaagc
    41641 ccggcgttga tgccaccgag aatatcggac tcggcagtgt cgccaaccat cagcacgcgt
    41701 gaacggtcag gattgcccgc ctgttccagc gcataatcga aaattttctt attcggtttg
    41761 gcaacgccaa cttcttcgga aatcaccagc aaatcgaagt aatcacgcag gcccgtccgt
    41821 tccagacgca cttgctgcaa ggcgctaaaa ccgttggtga tgatgccgat tttagcgttg
    41881 ctacgaatgg cgttaagcag agaaaccgcg cccggcaacg gcgtacagat ttccgccatc
    41941 gcattaataa aggcatcgtt aagcaagcct gcttcaactt tcagccgttc ggcccagctc
    42001 tcgaaacgcc cgtgctgaag ctgtaatgaa gtgatcgcgc cgttttgata atccacccac
    42061 agtggcttgt taacggcctg atagtcctga aaatcttcag cggtaaaggt gacgctgtaa
    42121 tcaagaaaca tccgctgcag gccggtgaat gagtcaaagg taaacagcgt ttcatcggca
    42181 tcaaagaaaa tccagtccca cttcattatt ccaccttgtc ttacatactg attggcaacg
    42241 ccatgatgat ggcgtcttcg cgaccgtccg tggtggggta gtaattgcgg cgaatcgtcg
    42301 cctcgttaaa gcctaaactt tcgtacaggg caatggcggc agcatttgag gcacggactt
    42361 ccagccatag tgtcgccacg ccgcgttttt ccagttcgtc gatcagatgt tccagcagcg
    42421 ccctacccag cccctgacgc tgatagtcgg gatcgaccgc aatattgaac aatgtagctt
    42481 catccagcac cacttgcgta atcgcaaacg ccgccatttt gccgttttgc gttaactgaa
    42541 agttgagata acgctcgccc tggttgctgg caaaggtttt ttcactccac ggaaaggcgt
    42601 gggcgcgttg ttcaatgtgg taagccgctg gtaaatcagt cgtttcgagg gaagaaatcg
    42661 tgttcatatg tgcaaatttg ttgccataac gcggcgcgtg ccgttgggtt tgcccgtaat
    42721 tcggtgagcg ccggtgatgc cacctgagcg ccttccaatg atagcggttc gtcagtaccc
    42781 aaccgccagc tgttgcagcg actgccttgc ggcagcatcg cgattttttc tggcgtcagt
    42841 tgcagcactt gatcggggct gacggttaat gcgcgcagaa catcgctcac taaaggatca
    42901 gtcagggcgg gaagatcgtt tgccaccatc accagacgga cgtgtgccgg gctcgcaatg
    42961 gcaatctccc cctgcaacgc gccagggcga cgcagcgacc actgggtaat gcccagttgc
    43021 tgtaactgcc agtctcgtcg ggatgtcata gcgaatcgct cctgttgtca ggggcgcaaa
    43081 tatagcaaat tcgtcgatac cgcgccaaca tatggctata atcgccgcca gtatcaattg
    43141 aggagcattc catgtctgca tttaccccgg caagtgaagt cttgctgcgt cacagtgatg
    43201 atttcgaaca aagccgtatt ctgtttgccg gagacttaca ggatgacctg cccgcgcgtt
    43261 tagataccgc ggccagccgt gctcataccc agcaattcca ccattggcag gtgttaagct
    43321 gccagatggg ggataacgcc cgttttagtc tggtcgccac ggcgaatgat gtcgcagatt
    43381 gcgatacgct gatttactac tggccgaaga acaaaccgga agcccagttc cagttgatga
    43441 atttactttc tctgctgcca gtggggacag atatttttgt cgttggcgag aaccgcagcg
    43501 gcgtgcgcag cgctgagcag atgctggcag attatgcgcc gttgaataaa gtcgacagcg
    43561 ctcgtcgctg tggcctctat tttggtcgtc tggaaaaaca gccagtattt gatgcggata
    43621 aattctgggg cgaatacagc gtcgatggcc tgacggtcaa aacgctgcct ggcgtgttta
    43681 gccgcgacgg tctggatgtc ggtagccagt tgctgctctc gacgttaact ccgcacacga
    43741 aaggtaaagt gctggatgtc ggctgtggcg cgggtgtact ttcagttgcc tttgcgcgcc
    43801 attcgccgaa aattcgtctc accttgtgcg atgtctctgc cccggcggta gaagccagcc
    43861 gcgcaacact tgcagccaac ggtgttgaag gtgaagtctt tgccagcaac gtcttttccg
    43921 aggtgaaagg ttgttttgat atgatcatct ccaacccgcc gttccacgat gggatgcaaa
    43981 ccagcctgga tgcggcgcaa acgctgattc gtggcgcggt gcgtcatctt aatagcggcg
    44041 gcgagctgcg aattgtagcg aacgccttcc tgccttaccc ggacgtgctg gatgagacat
    44101 ttggcttcca tgaagttatc gcgcaaaccg ggcgcttcaa ggtatatcgc gccattatga
    44161 cccgccaggc gaagaaaggt tgagtatctg atatctgtcg ccgcataccg tgtcggatgc
    44221 ggcgtgaacg ccttatccga cctacaaaac ctgcacgtta gcccttcgta ggccagataa
    44281 gacgcgccag cgtcgcatct ggcatctgcc cccgcatgcc taccaaacct gcacgctagt
    44341 ttcctgatgg acatttttcc agcaattaca catcaggcga taattaacta ttgacgaaaa
    44401 gctgaaaacc actagaatgc gcctccgtgg tagcaattct ttttaagaat tgatggtatg
    44461 cgaaggtggc ggaattggta gacgcgctag cttcaggtgt tagtgtcctt acggacgtgg
    44521 gggttcaagt cccccccctc gcaccaaaaa ccacgttgat attgctcgca ctgggcgaag
    44581 gtggcggaat tggtagacgc gctagcttca ggtgttagtg ttcttacgga cgtgggggtt
    44641 caagtccccc ccctcgcacc aaacgaggcg atatcaaaaa aagtaagatg actgtgcgaa
    44701 ggtggcggaa ttggtagacg cgctagcttc aggtgttagt gtccttacgg acgtgggggt
    44761 tcaagtcccc cccctcgcac caattatctt tacttccttt cttgtttctt ccctgttttg
    44821 tcatccctga ttcaaattca ttatcatcag catgatgcca gcaataagcg ccaggcacga
    44881 tggtagcaaa atgtaatgcc gcaattgttt gtgatacagc atcaccggcg tcaatactaa
    44941 tcccatcaga atgacaatcc gccactgatc cggcgataaa tccgcataca tcagccctgc
    45001 aatcagcaat cccggcagca acactcccca gccactgccc agcgtacgtt gcaacatggt
    45061 ttcatctctt tcattgataa tgaaaaccaa tatcatatga taatttttat catttgcaag
    45121 ccagagaaat cccttgctat cgagtaaacc tatcgctagg attagcaatc attatcattt
    45181 agattactat cacgattatg gcctatcgtt ccgcaccgct ctatgaagat gtcatctggc
    45241 gaacgcatct ccagccgcag gatgctgggc ttgcacaagc cgtacgggcg atgatcgcca
    45301 aacatcgtga acatttgctg gagtttatcc gcctggatga acccgctccg cttaacgcca
    45361 tgacgctggc gcaatggtca tcaccgaatg cgctaagttc tctactggcg gtctattccg
    45421 atcatatcta tcgcaaccaa ccaacgatga tccgcgagaa caaaccgctg atctcactat
    45481 gggcacaatg gtatatcggc ctgatggtgc caccattaat gctggcgcta ctgacgcagg
    45541 aaaaagcatt agatgtgtcg ccggaacatt tccatgctga gtttcacgaa accggacgcg
    45601 tcgcctgttt ctgggtcgat gtgtgtgaag ataaaaacgc aacaccacat tcgccgcagc
    45661 agcgaatgga aacgttaatc agccaggcgc tggttccggt tgtgcaagca ctagaagcga
    45721 ccggagagat caacggcaaa cttatctgga gtaataccgg ttatttgatc aactggtatc
    45781 tcactgagat gaagcaactg ctcggcgagg ctactgttga atcgctgcgc catgccctct
    45841 tttttgagaa aacgctcacg aacggtgaag ataatccact ctggcgtacc gtggtactgc
    45901 gcgacggcct gctggtgcgc cgcacttgct gccagcgtta tcgcttaccg gatgtgcagc
    45961 aatgtggcta ttgtacgctg aaatgaaagc actgccgggg aagtaaaccc ggcatcatgc
    46021 ggattaatag ggatgcaaca cattacttgc ttcgcaatgc cgcatacata acagaatatc
    46081 tgcggcctcc aacaaccctg catcagaact gacgcccagt ttcgacatca cattaaattt
    46141 atgcgctcgg atcgttttga tattacgttt aagctgctcg gcaatttgtg tcattgagta
    46201 gccacgcgac ataaagcgca atatttctct ctccgtcggg cttaacgtcc ggctttggtt
    46261 aatgtaccac tgattgttca gtcggtcggt cgcctgacgt acaccattta atgacaaaaa
    46321 gagttcctga tgaaaaatct ccagcgttga cgctttactt aatacaccgt ccagcggtga
    46381 tggcgacaat gaaccaatca gccgagcttc aatatcatca tccacaataa ctaaacgccg
    46441 ggtacgcgga aacttaatcg ccagctcagt caggcaagat aatccctctc tgcgctcact
    46501 tctcatggca gaaaaagaaa aaatgaccgc cgcaaacgag atccgcgaca tggcatcctg
    46561 aaatgactcc tgactggtat aggtatggag cttataaggg ttgcccgcaa actttctgaa
    46621 taaactctca ataccgatac tactcatgac gcatttttct atgagtgcga catttctctt
    46681 cttaattcgg ctatgttcca ttctgcggca tctccataaa cagaaggcaa gttaagttct
    46741 cttgcaccct gcgcgctatt tatccatgca tacatttcag cgttgctgtg aattgccagt
    46801 cgacgcatcg cactgttttt ttgggcgctg atagttttat tacttttctt aagcaatgaa
    46861 gctatctggt tgatgcccca tcctttacct aatagccgca gtaccttgcg ctctgaaaga
    46921 gtgagaatga cggagcgata gctaaaatca tgagtttcag tcattgccgg agaggtcagc
    46981 atggcgctga tacgctctgc gtgcgtattg ccggaacgta cggtcttgac cagattctca
    47041 atgggttcaa catcagacaa taacgtcgcc gtagggcaca tgagcagttc tacagcctgg
    47101 gaataccagg agcgtgacac catgaaaacc cagtgaattt cccgatattg tgagattaaa
    47161 gaataataat gttcacaaac agaacgtgga tcttcacttt caccggctaa atcagcaatg
    47221 actaat
//
LOCUS       DAJTRB010000023        46320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTRB010000023 DAJTRB010000000
VERSION     DAJTRB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.023"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            40..1770
                     /locus_tag="OO330_001379"
     CDS             40..1770
                     /locus_tag="OO330_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5882303.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMSPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            1767..2681
                     /locus_tag="OO330_001380"
     CDS             1767..2681
                     /locus_tag="OO330_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5882304.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2714..2962
                     /locus_tag="OO330_001381"
     CDS             2714..2962
                     /locus_tag="OO330_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5882305.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2962..4133
                     /locus_tag="OO330_001382"
                     /pseudo
     CDS             2962..4133
                     /locus_tag="OO330_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(4169..5248)
                     /gene="lptG"
                     /locus_tag="OO330_001383"
     CDS             complement(4169..5248)
                     /gene="lptG"
                     /locus_tag="OO330_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5882306.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5245..6315)
                     /gene="lptF"
                     /locus_tag="OO330_001384"
     CDS             complement(5245..6315)
                     /gene="lptF"
                     /locus_tag="OO330_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5882307.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(6346..6906)
                     /locus_tag="OO330_001385"
     CDS             complement(6346..6906)
                     /locus_tag="OO330_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5882308.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6918..7742)
                     /locus_tag="OO330_001386"
     CDS             complement(6918..7742)
                     /locus_tag="OO330_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882309.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(7742..9091)
                     /locus_tag="OO330_001387"
     CDS             complement(7742..9091)
                     /locus_tag="OO330_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5882310.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9137..9895)
                     /locus_tag="OO330_001388"
     CDS             complement(9137..9895)
                     /locus_tag="OO330_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882311.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(9927..10640)
                     /locus_tag="OO330_001389"
     CDS             complement(9927..10640)
                     /locus_tag="OO330_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5882312.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            10814..11506
                     /locus_tag="OO330_001390"
     CDS             10814..11506
                     /locus_tag="OO330_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5882313.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11555..13054)
                     /gene="pitB"
                     /locus_tag="OO330_001391"
     CDS             complement(11555..13054)
                     /gene="pitB"
                     /locus_tag="OO330_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5882314.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13346..15205)
                     /gene="gss"
                     /locus_tag="OO330_001392"
     CDS             complement(13346..15205)
                     /gene="gss"
                     /locus_tag="OO330_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5882315.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15410..16276
                     /gene="yghU"
                     /locus_tag="OO330_001393"
     CDS             15410..16276
                     /gene="yghU"
                     /locus_tag="OO330_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5882316.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16399..16647)
                     /gene="hybG"
                     /locus_tag="OO330_001394"
     CDS             complement(16399..16647)
                     /gene="hybG"
                     /locus_tag="OO330_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5882317.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16781..17122)
                     /gene="hypA"
                     /locus_tag="OO330_001395"
     CDS             complement(16781..17122)
                     /gene="hypA"
                     /locus_tag="OO330_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5882318.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17115..17603)
                     /gene="hybE"
                     /locus_tag="OO330_001396"
     CDS             complement(17115..17603)
                     /gene="hybE"
                     /locus_tag="OO330_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5882319.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(17596..18090)
                     /locus_tag="OO330_001397"
     CDS             complement(17596..18090)
                     /locus_tag="OO330_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5882320.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18090..19793)
                     /gene="hybC"
                     /locus_tag="OO330_001398"
     CDS             complement(18090..19793)
                     /gene="hybC"
                     /locus_tag="OO330_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5882321.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19790..20968)
                     /gene="hybB"
                     /locus_tag="OO330_001399"
     CDS             complement(19790..20968)
                     /gene="hybB"
                     /locus_tag="OO330_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5882322.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWSLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20958..21944)
                     /gene="hybA"
                     /locus_tag="OO330_001400"
     CDS             complement(20958..21944)
                     /gene="hybA"
                     /locus_tag="OO330_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5882323.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21947..23065)
                     /gene="hybO"
                     /locus_tag="OO330_001401"
     CDS             complement(21947..23065)
                     /gene="hybO"
                     /locus_tag="OO330_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5882324.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23254..23541)
                     /locus_tag="OO330_001402"
     CDS             complement(23254..23541)
                     /locus_tag="OO330_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5882325.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(23660..24547)
                     /locus_tag="OO330_001403"
     CDS             complement(23660..24547)
                     /locus_tag="OO330_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5882326.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24704..25744
                     /gene="gpr"
                     /locus_tag="OO330_001404"
     CDS             24704..25744
                     /gene="gpr"
                     /locus_tag="OO330_001404"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5882327.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25784..26278)
                     /locus_tag="OO330_001405"
     CDS             complement(25784..26278)
                     /locus_tag="OO330_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5882328.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26469..27353
                     /gene="yghA"
                     /locus_tag="OO330_001406"
     CDS             26469..27353
                     /gene="yghA"
                     /locus_tag="OO330_001406"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5882329.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISSGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27625..28050)
                     /gene="exbD"
                     /locus_tag="OO330_001407"
     CDS             complement(27625..28050)
                     /gene="exbD"
                     /locus_tag="OO330_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5882330.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28057..28791)
                     /gene="exbB"
                     /locus_tag="OO330_001408"
     CDS             complement(28057..28791)
                     /gene="exbB"
                     /locus_tag="OO330_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5882331.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29043..30230
                     /gene="metC"
                     /locus_tag="OO330_001409"
     CDS             29043..30230
                     /gene="metC"
                     /locus_tag="OO330_001409"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5882332.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30370..31029
                     /locus_tag="OO330_001410"
     CDS             30370..31029
                     /locus_tag="OO330_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5882333.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31069..31968)
                     /gene="yqhC"
                     /locus_tag="OO330_001411"
     CDS             complement(31069..31968)
                     /gene="yqhC"
                     /locus_tag="OO330_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5882334.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32162..33325
                     /gene="yqhD"
                     /locus_tag="OO330_001412"
     CDS             32162..33325
                     /gene="yqhD"
                     /locus_tag="OO330_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5882335.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33430..34257
                     /gene="dkgA"
                     /locus_tag="OO330_001413"
     CDS             33430..34257
                     /gene="dkgA"
                     /locus_tag="OO330_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5882336.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            34457..35383
                     /locus_tag="OO330_001414"
     CDS             34457..35383
                     /locus_tag="OO330_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5882337.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35434..35691
                     /gene="yqhH"
                     /locus_tag="OO330_001415"
     CDS             35434..35691
                     /gene="yqhH"
                     /locus_tag="OO330_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5882338.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35734..37953)
                     /locus_tag="OO330_001416"
     CDS             complement(35734..37953)
                     /locus_tag="OO330_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5882339.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(38064..39476)
                     /gene="ftsP"
                     /locus_tag="OO330_001417"
     CDS             complement(38064..39476)
                     /gene="ftsP"
                     /locus_tag="OO330_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5882340.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKDDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39551..40288)
                     /gene="plsC"
                     /locus_tag="OO330_001418"
                     /gene_synonym="parF"
     CDS             complement(39551..40288)
                     /gene="plsC"
                     /locus_tag="OO330_001418"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5882341.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40522..42780)
                     /gene="parC"
                     /locus_tag="OO330_001419"
     CDS             complement(40522..42780)
                     /gene="parC"
                     /locus_tag="OO330_001419"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5882342.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(43326..43808)
                     /locus_tag="OO330_001420"
     CDS             complement(43326..43808)
                     /locus_tag="OO330_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5882343.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(43861..44253)
                     /gene="ygiW"
                     /locus_tag="OO330_001421"
     CDS             complement(43861..44253)
                     /gene="ygiW"
                     /locus_tag="OO330_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5882344.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            44405..45064
                     /gene="qseB"
                     /locus_tag="OO330_001422"
     CDS             44405..45064
                     /gene="qseB"
                     /locus_tag="OO330_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5882345.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            45061..>46320
                     /gene="qseC"
                     /locus_tag="OO330_001423"
     CDS             45061..>46320
                     /gene="qseC"
                     /locus_tag="OO330_001423"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5882346.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMIRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEA"
BASE COUNT        11486 a        12291 c        11605 g        10938 t
ORIGIN      
        1 cgcattattg tcattttatt tttgatatcc aattttttcg tggtgtatat gtctaataaa
       61 atctttacgc attccctacc tatgcgctat gccgattttc caacgctggt tgatgctttg
      121 gactacgccg ctctgagtag agccggaatg aatttttatg acagacgttg ccaacttgaa
      181 gatcaactgg aatatcagac attaaaaacg cgtgccgaag ctggtgcgaa gcggttgtta
      241 tcgctgaacc tgaaaaaagg cgaacgcgtg gcgctgattg ccgaaacgag tagcgggttt
      301 gtagaggctt tttttgcctg tcagtatgct ggcttagtcg ccgtcccgtt ggcgattcca
      361 atgggcgtcg gtcagcggga ttcctggagc gccaaactcc agggtttact ggcaagttgc
      421 cagcccgcag ccattatcac tggtgatgag tggttgccac tggtcaatgc cgcgacgcat
      481 aacaaccccg aattacatgt tttaagccac gcttggttta aggcattacc ggaagccgat
      541 gttgcgctcc agcgtccagt tccgaacgat atcgcctacc tccagtacac ctccggcagc
      601 acccgttttc cccgtggcgt cattatcacc catcgcgaag tgatggctaa tctacgtgct
      661 ataagccacg atggcattaa attacgccct agcgaccgct gcgtctcctg gcagcctttc
      721 taccatgata tgggactggt cggctttctc ctgccccccg tcgccacgca actttcagta
      781 gattatttgc gcactcagga ttttgccatg agtcctttgc aatggcttaa attgatcagt
      841 aaaaatcgcg gcaccgtttc cgttgcgccg ccgtttggct atgaattgtg ccaacgccgc
      901 gtgaatgaaa aagatctcgc tgaactggat ctttcctgct ggcgcgtcgc tggtattggt
      961 gctgagccga tctccgcaga acaactccat caattcgctg aatgtttccg tcaggttaac
     1021 tttgacaata aaactttcat gccgtgctac ggactggcag aaaatgcgct ggctgtcagc
     1081 ttctctgatg aagcctccgg ggttgtggtt aacgaagtgg atcgcgacat cctcgaatat
     1141 cagggtaaag ccgtcgcgcc gggtgcagag acacgcgccg tatcgacttt cgtcaactgc
     1201 ggcaaagcgt tgccggaaca tggtattgaa atccgcaatg aagcaggtat gccggtcgcg
     1261 gaacgtgtgg taggccatat ttgcatctcc ggtcccagtc tgatgagcgg ttactttggc
     1321 gaccaggttt cgcaagacga gattgccgcg acgggctggt tagacaccgg cgacctcggt
     1381 tatctgctgg acggttatct gtatgtcacc ggacgcatta aagatctgat tattattcgt
     1441 ggccgtaata tctggccgca ggatattgaa tatatagcgg aacaggaacc ggaaattcat
     1501 tctggcgatg cgattgcttt tgttaccgcc caggaaaaaa tcattttgca gatccagtgt
     1561 cggatcagcg acgaagaacg tcgcgggcag cttatccacg cgctggcggc acggatccaa
     1621 agcgaatttg gcgtgaccgc ggctatcgat ctgttaccgc cccacagtat tccccgaacg
     1681 tcctccggca agcctgcccg tgcggaagcg aaaaaacgtt atcagaaggc ttatgctgcc
     1741 agtcttaatg tgcaggaatc cctggcataa atcaaactgt cgcggtgacg ggcgctaccg
     1801 ggtttatcgg taaatatatt atcgataacc tgctcgcccg cggctttcac gttcgcgcat
     1861 tgacgcgtac tgcccgcgct cacgtcaatg ataatctcac ctgggtgcgc ggttcgctgg
     1921 aagatactca ttcacttagc gagctggttg ccggagccag cgtagtggtc cattgcgccg
     1981 ggcaagtgcg cgggcacaaa gaagagattt tcacccgctg taacgttgac ggcagcctgc
     2041 gcctgatgca agcagcaaaa gagagcggct tttgccaacg ttttctgttt atctcttcgc
     2101 tggcggcgcg ccatcccgag ctctcctggt atgcaaaatc caaatacgtc gccgagcaac
     2161 gactggcagc aatggcggac gaaattacgc tgggcgtttt tcgcccgaca gccgtgtatg
     2221 gtcccggcga taaagagtta aaaccgctgt ttgactggat gctacgcggc ctgctgccac
     2281 gacttggtgc gccagataca caactctctt ttcttcacgt caccgatttt gcgcaagcag
     2341 tgggtcagtg gttaagcgcc gaaactgtac agacgcaaac ctatgaatta tgcgatggcg
     2401 tcgccggtgg ctatgactgg caacgcgtac gacagcttgc cgccaaagcc cgttgtggtt
     2461 ccgtgcgaat ggttgacatt cctctgccgg tactcacagg ccttgcggat atcagtaccg
     2521 cgttgagtcg cctggcaggt aaagaaccta tgctgacccg ctcgaaaatt cgtgaattaa
     2581 cccacgccga ctggtcggca agtaataacc gtatttctga agatatcaat tggtttcccg
     2641 ggattagcct ggaacacgca ttacgcaacg ggctattttg attgtcttgt tttaaaggtg
     2701 tgttaacgtt tctatggtaa atcgtgaaat agtaatggat tacatccttt cctgtttaca
     2761 ggatttggtc gaaaacggtg tggaaattaa gcccgacagc gatctcgtca acgatcttgg
     2821 cctggagtcg atcaaagtga tggatctctt aatgatgctg gaagacagat tcgatatttc
     2881 cattcctatt aatattctgc tggatgtcaa aacccctgcg caattaatgg aaactctact
     2941 tccatggctg gagaataaat aatggggcta tacgataaat atgcgcgcct tgctggcgag
     3001 cgtctgcaat ttagtgacaa cgggttaaca ccgtttggca cctgtattga tgaagtgtat
     3061 tccgccaccg aaggccggat cggcaataaa aaagtgattc tggccgggac caataattat
     3121 ctcggtttaa cctttaatca tgatgccatc agcgaaggtc aggccgcgtt agcggcccaa
     3181 ggcaccggca ccaccggttc tcgtatggca aacggcagtt atgcgccgca cctggcgtta
     3241 gagaaagaga ttgccgagtt tttcaatctc cctaccgcta tcgtttttca accggttata
     3301 ccgctaattt aggcgtcatc agcgcacttg ccgaccataa cgccgtggtg ttactggatg
     3361 ccgacagcca cgccagtatt tatgacgcct gttctctcgg tggcgcggaa attattcgtt
     3421 tccgccataa cgatgctaaa gatctggaac ggcgcatggt tcgcctcggt gaacgggcga
     3481 aagaggcgat tatcatcgtt gagggcatct acagcatgct cggtgacgtt gcgccgctgg
     3541 cggaaattgt tgatatcaaa cgtcgtctcg gtggttattt gattgtcgat gaagcgcatt
     3601 cgtttggcgt gttaggcgca acggggcgcg gactggctga agctgtcggc gttgaagatg
     3661 atgtggatat tatcgttggt actttcagta aaagtctggc ctcgattggc ggttttgctg
     3721 tcggttcgga agcgatggaa gtgctgcgct acggcagccg cccgtatatt tttaccgcct
     3781 ctccttctcc gtcctgcatt gcgaccgtgc gctcgtcgtt aagaactatt gccacacaac
     3841 cagagctgcg gcaaaagctg atggataacg ccaaccatct gtatgacggt ctgcaaaaac
     3901 tgggttatga gttaagttca catattagcc cggtagtgcc agtaataatc ggttcgaaag
     3961 aggaaggttt acgcatctgg cgcgagctta tctcgccggg cgtgtatgtg aatcttatcc
     4021 tgccgcctgc cgcgcctgca ggcatcacgc tgctgcgctg tagcgtaaat gctgcccaca
     4081 gtcgtgagca aatcgatgcc atcattcagg catttgctac tctgaagcag taatgtgagc
     4141 ccgccgtggt gtttgcgcca cggcgggttt aatgttgtct gtcataccaa tacaccaggc
     4201 tcaacgccac caacacaaac gctaccggta aaccgagcgc ggtaacaggt gcgcttaacg
     4261 caaatagcaa tcccagatta accatgattt gatagctgat ccaggtgagt aagcccacga
     4321 tgacgcctac agcgagacgg ctacccatcc caggcgaacg cggggcacta aaggtaaacg
     4381 gcacagccag caaaatcatc gccagggtca ggatgggttg ccccagtttt tcccacagtg
     4441 caaggcggta ttcgctgctg ggttgcccgg tttcctgcaa ataatggatg taatgattaa
     4501 gctgacggac agaaaaagta ttccccggca ttgataactc ttcaagattc atactggtga
     4561 aggccgattg ccaggcaaga ttatctaatg tttccagcgt ttctttgcca ttcagccatt
     4621 ttttatgatt cacaccatgc aggacccacg ttttgtcgtc ttcaatggtg gcactgcgtg
     4681 cataaatgta ggattcaagc gagagatcgt cgcgataatg aaatatctcc acgcccaccg
     4741 gctggccctg ctcgttcagc gatttcaccg tcacaaattc attaccacgc ctggcccaca
     4801 gcatattgcc ggtaatgtca tcgtcctccc ccaacgcggt ggcggtcgat ttgatttgca
     4861 acgcctgttg ctgcaatggc gacgccaccc actcatctat cgcgcctaac gaaacagtcc
     4921 acaatatccc ggcaaccagc gccaccagtg caatacggaa gatagaaaac cccgtgctgc
     4981 gaatggcggt aagctcactg tttttcgata actgccccag tccaacaata ccacccaata
     5041 acgcgataaa cggcgaaagt tcgaccagtg tgcgcggtaa ggtcattagc accaccagca
     5101 ccgcctgagt ccagcgataa ccgcccgggc tgacaccatc cagttcgtta atcaggttga
     5161 aggtggtaaa aagcggcagc aatagccctg cagcggcggc aaaaccgaga aagagatgac
     5221 ggattaaata gcgactgaaa acattcatcg ggaaaatttc tgcagcaaag agaagtcacg
     5281 tgcgaccagt atcagcaggc ccattagcat gagtccgggc actaaccaca aaccaggaat
     5341 gaggggaatc gcgccgttag ccaccagtgt acggcagacg tcgccgccat aaaaaatggc
     5401 aataaacagc aacgtcagtg gcaataacgt tgaaaatcgc ccttgccgcg gttttacccg
     5461 acttaatgaa atggccagca gcgccatcaa caatgcggtc aggccacggc tttgtcgcca
     5521 ttgcagttcg gcatgatcgg caggaaaggc ggagcgcgcc agctccgtga ccgatttcgc
     5581 tttgcgtttg acgttcgggc tttgatccag cggattcaga tgtaattgca gattacggta
     5641 gatctgttcg ttgtcgtcac gcccctgatg atcgagaaga taggcagtgc cgttgtgcaa
     5701 cataacggtc ggtttctcgg gtgatgggtc aaccacatca accgaaccgg cgcggaaaat
     5761 acgggttcga ttggcagtag aagtataaat cagcgcgtca gtcaggcgat tattatcctg
     5821 atcaaccgtc tgcgaaagga tcattcgtcc gttatcgtta gtgttaaatt tctttgcccg
     5881 caactggcga acatccagct ccgactgcga ctgctgctcc agttgataaa tctgcgcata
     5941 agcccacgga cgaccataca tcgacagaag ggtgacaaaa atacttagcg gtatcgccag
     6001 ataaagaacg gctttgtaca agcgtcccgg actgcccccc gccgcagaga tagcggtaat
     6061 ttccgagtcg gtatacatct gccctagcgt cacgccaact gacacataca gaccaacagg
     6121 taacaacatc tccagtgcaa tcagtacttt gtagaaaacg atatccagca caacatcaag
     6181 agctaatgtc ccatttgccg cttcggtcag ataacgctgt gcggagtagc tggcgaaaat
     6241 aaagatcaga aaaccgacga taatcagcac cagacggcgc gtaccacgca tgatgtagtg
     6301 ttcaaccagc ttcatctcac taacgccctc aatcctttga gttttttatt cattgatcag
     6361 tttctgcgcc aacgcaatga catgatctac ctcttccggc gtcaggctgc cgtcctttgc
     6421 ccactgaatt tgcccggctt tattcagcac cagaatggtt gaactctgct ccggcaagcg
     6481 ccaggcaacg cgtccctgtc cattgccgtc aataacgaat tgcgcccacg gatagcggcg
     6541 tttatttttt tcgattttgc caacaacaaa atagccagta ccaaagatgg catcatcggt
     6601 gttcacaatg gtggtgggct gaaaacgatc ctgcggaaag tttgccgctt caaccgcttt
     6661 aattaacagt gagttctttt tcttcgccga cttacgcccg gcgatatact gaataatgcg
     6721 gacttttccc gctaattgtg tgctgttcca cttttggtag tgaatttcgt cgtcgctatt
     6781 cagaagcagc tcgccgcctt cctggatata aacaggggta accgttttgc cagtgacgaa
     6841 attgtgtgcc tgcacacccg gggctaagag catgataatc aacgcaataa tcaaacgaga
     6901 tgacatcatt gctccgccta tagcggaaaa cgcgaaatca gcagctctaa gcgctgacaa
     6961 atatcgctat ccagcgccaa cggtgcataa tacgactggc ggttttccat atcatccgcc
     7021 accatgtcgt cgtgtttatc tcccatcagg ccattaatgg cggcgaggaa acgggaaatc
     7081 tgtaacgcct ctttattttc aggatcaagc gatcttaagg tgtcgtaata agaaacctgc
     7141 tggctgatga tttttcgcac atcatgaaat tccctgccgg ttagtcgcgg tttggcaata
     7201 atctctttca gggtatgcat ttgcttttgc cgataggcaa taaaactgct tacgctttct
     7261 aactggctat ggcgtaatga atagttcacc aacgaccaga ccggagagct taaataaatc
     7321 cgtaacaggt tgccgtaata cgacacaata tttttcttac cgttacgaaa gccgtcctga
     7381 aaacgcccca gaaaaacggt cgttttgccg aataaaaatc cggcctgatg cttctttaaa
     7441 tacagccgtt gcgcaaaacg caggtgcttg tagcgcgctc gcatgtattt aaattgtttg
     7501 cgctcatcag cagtcagctc atcgcctttg gcttgtttgc gaactaaacg cagtaattcc
     7561 tgacgatcaa cgccattgag ccagaattgc aggcataacg cgtaattatc atgaatgatt
     7621 tcggtggtac aacttagtgc aatagtcggc ggcaaactaa cctgtgcatc aacgacatca
     7681 tcttcttcaa tcgcaacaaa tagacgctcg ctcgcctcaa gcgggaaata ttttctcatc
     7741 atcaggattt ctctccggct gcgttataaa gcaaggaatg gcgctgaaaa aatgccttta
     7801 aggttggaat taacgagagc gccacgtcca gacattgccc accgaagtaa gcaaacgctg
     7861 ctcccagcac accaaacaga tgacagaaca tgaacagcaa tacgatgtag ccgattgagg
     7921 cgatggtttg cgccacgaga aaagcgcgct gttttcccgc catcaatagc aatgactcct
     7981 gcgggaagcc cagcatcgaa atcacaattg cgcccagcat cacctgaatc aggtcatacg
     8041 cttcgagata cttaacgcca aacaccagcg aaatgagcgg cttgccgacg atcaacaccg
     8101 cgagcgccac cagaataccg attccgcccg ccagtaaacc ggacttcaca cctaacaacc
     8161 acggtctggt ggtgcgcgga tctaaacgca tcacctctgg gtaaaaactt ttacccagca
     8221 aacccgctgg cgttccggcg gcgtcgaaga atgtcatggc gattttaaat aacccggcgg
     8281 cagcgggtcc taacacgatc cccaccaaca ctgtgctgca cgagttacgc gccgaccaga
     8341 tggagtgggc aatgttggtt gaccagacaa aactccacgc gcctttaatg tgtcgggcag
     8401 actcaaacag attcaatttg aaggcgttat ggatatttct acggcgtaat tcgcgcgcgg
     8461 caaaccacca gtacatggtg ccgccaacca gattcgacac gtaccaggca ataacaaaac
     8521 ccgcaaaacc aaagtcaaaa taccaggctg cgacgctccc cgctgcgcgc agaaaaggtt
     8581 tcgtcgcctg ctgtacagca atcaaatcaa agcgatctac cgcacgcaga atgccggtcg
     8641 gcgtggagga agccattgaa ggaatgagcg tgcaatacag cgccgccagc caaaagcttt
     8701 ggtcatccag acctaatgaa tgggaaagga atggcagtaa ggcaatgccg ccgacaatcg
     8761 ccaccgcgcc gctgacgata tccagcgaga aggaaaatga gacgacattg cgaaattgct
     8821 gcggattatt gttggtcaag gctggcgttc cgtactgaac caccagctgc catgtctgaa
     8881 acttaataaa atcgctgatc gacttggcgt acgattgcac aatcaccagt acaccaaaca
     8941 tggcgggcgt catcccttta ccggcacacg agagcgccaa cagccccagc aaggcgctct
     9001 cgacattact ggagcctaac caggcgctat tgcgaataat ggtgcgaaac gcgccatctg
     9061 caaaccaatg tttgatgtta aaacccgcca attcagcctg accttatcgt tgatagtaaa
     9121 aaatatcccg ccagccttaa gttaaacttc ggcggtcaga aacgatggca accagagaaa
     9181 ccgccttctg tacctgttcc agcacttcgc tgtagggcgc tctggaatca atctcaagaa
     9241 tttttgtgcc gttatagcca atcgtcgaca tgacgccgat tttgtcctgc agctcggcat
     9301 actcatggtc aggcttgcgg gagatggcag tatcaatatc aatccccaaa cgaatgatta
     9361 attcaggacg gtattgcgcc attttttggt ataaactccg ttcgcgctgc gccagaaaca
     9421 tgctgatttt cccggtcgca cgttcgacgc cgatccccgg tccgtcataa taaaagcccg
     9481 aaatttcagc ctgcgggaag cgatcgctga ccaccagaac gccactttgc gccagtcgct
     9541 gaaccttgcg tagattcgcc attcttcgca gcgagaagca gtacataatc accgccgccc
     9601 atagcgccgg agatttggtt ttcatgcttt gggttttcga ggatttggcc gccaatcgcc
     9661 gttccagcca gacgccaacc aacggcaatc gtttgatttt gtcgccgtct tcgccggaga
     9721 gcagccccag atagcggcgc tcggtttgcc agtgttgttg cagcgatttc accaggtcgg
     9781 tggtcagcgt ggatttaccg gtgccatcac acccgaccac cgcaatcagc cccggaatgt
     9841 agttgggctg cggcgcggtt gtactattaa cagtttgtgt ttgtagtgca tccattcgtt
     9901 aaatcatccc ctatccattt tggtgatcag gaaagcgcgg cgtgaattgc gcgtagtgac
     9961 gcttgcataa tttcatcggc tggttgccga ccatccagtt caaggatctt ggcgccattg
    10021 aatgtcagtt gcggcgtaac ggcgattttt tcctgtaacg ctgccagttg gtggtcaggt
    10081 ttacgcgcaa acgcggtttg ttcatcaatg ccaagacgaa tcaacaatac gggtaaataa
    10141 gacgccatcc attggtacag cttcaactcg cgctgcctta acatttttat ccaaccgtta
    10201 ccgcccgtgg tttttgccaa ttgcgggcca tcaaagcgaa aacccggcac ttcaacctgc
    10261 gggtagcggt cggtgatgag cagatagcct tgctggcttt tacacaacat tttgcgaaac
    10321 ttgtacgccc gccagcagga aagcagatag ataaccagcg cagtaatatt gccaggcgga
    10381 gttaagggct tttcgtgtac atgtgccgct ttacttcgca gataacaccc acaaggtgcg
    10441 ccaataacag ggagctgtga aatccattcg ccaattcgcc cggacgattg cccgagataa
    10501 atgtgttctg ttggcattct tgctgccagt tcatttacca ggcttgccgt gagggtcgat
    10561 ttacctgaac catcacatcc tacaatggca ataacacgca ctggcgtgga attaattatt
    10621 gacatattac gttgattcac gaaaaacccg gcagtaaata atgtattgaa tattacgtgg
    10681 tccgtgctca gaatatccgt tcaaccttat tgtgacaatg gctaattatg acattgacct
    10741 gaattacgct ttcataaaaa catattaacc aaataaatat ttttaataga tatttaaatt
    10801 aaaggatata ttcatgcagt caataacacc tccattaatt gccgttattg gtagcgatgg
    10861 ttcaggcaag tcaacggtgt gtgaacatct tattaccgtt gtcgaaaaat atggtgctgc
    10921 cgaaagagtt catttaggaa aacaggccgg aaatgtcggt cgtgcagtga caaaattacc
    10981 tttgatggga aaatccttac ataaaacaat tgaacgaaat caggtgaaaa cagcaaaaaa
    11041 attgcctgga ccagttccgg cgctggtaat tacagcgttt gtcgcccgtc gcttactgcg
    11101 ctttcgtcat atgcttgcct gtcgtcgtcg cgggttaatt gttctaaccg accgttatcc
    11161 tcaggaccaa attcctggcg cttacgatgg tacggtgttc ccacctaacg ttgaaggtgg
    11221 tcgttttgtc tcatggctgg caagccagga acgtaaagcg tttcactgga tggcgagcca
    11281 taagcctgat ctggtcatca aactcaatgt tgaccttgaa gttgcctgtg cacgtaaacc
    11341 cgaccataaa cgggaatcgc tggcgaggaa gattgccata acgccacagt taacctttgg
    11401 tggtgcacaa ctggttgata tcgatgccaa tcagccactg gaacaggtgt tggttgatgc
    11461 agaaaaagcg attacggatt ttatgaccgc gcgtggttat cactagtcaa aaatggaaat
    11521 gcccgatcgc caggaccggg cattttcagg aaggttaaat caactgcaat gctatccagt
    11581 acagcccacc agaaagaaaa attgccgccg gtaaagtaaa cacccacgcc atcaggatgc
    11641 tggttaccgt tttacgctgt aacccaccgc cgtccaccac catcgtccct gctaccgcag
    11701 acgagaggac gtgtgttgtg gagacaggca tcccaatata actggcaagg ccgatagaca
    11761 ctgccgccgt catttgtgcc gccatgcctt gcgcatacgt catgccgcgc ttaccaatct
    11821 tctcaccgat ggtcatcgct acacgacgcc agccaatcat ggtgccaatg ccgagcgcca
    11881 gtgctaccgc catgatgatc cacaccggag cgtactcaat ggtgcttaac atatcgctgc
    11941 gcagtttttt cagcaggttc tggtcttctt tactgacgcc tggcagtttc gccagcttcg
    12001 cggaggtatc ggagatgcac agcataatgc ggcgcagctg gctgcgctga ctcacgctta
    12061 acggctcgta actttccata ttgcctggca gcatcgtttt aacgcgcgca atcgcatcaa
    12121 aggtatttgc cggatgacag tgaaactctg ttacttgcgt gccatcagtc gatgctgcag
    12181 gcaatggagg ttccatcgca atcaacttct gcggcagttc aggatgctgt tgcaggtagt
    12241 gttcgaagtt ggtaacggca tcgcgggtac gggtaatttc atagccggac gcattcatat
    12301 tgacgacgaa gccagcaggg gcaatcccca ccagtaccag cattaccagg ccgatccctt
    12361 tctgcccgtc gttcgcgccg tgcgaaaacg ccacgcccgc agcggaaaca atcagcgcaa
    12421 tacgcgtcca gaatggcggt ttacgtttgc cctttttctt tttgcgatct tccggaatgc
    12481 ggtgaatacg gtcacgcttt ttcgtcccac tccagaaacg gcgcagcagg aaaatcagac
    12541 cacccgcaat gaccaggccg acgatagggg aaacaatcag cgaggagaaa attttggtta
    12601 cttcacgcag gtttaacgca tccatcaccg atgagccggt taacagcgcg ttggttaaac
    12661 cgatgccgat aatcgcacca atcaaggtgt gcgaactgga ggccggtaaa ccaaagaacc
    12721 acgttcccag gttccagata atcgccgcca gcagcatgga aaagaccatc gccaaaccgt
    12781 gagttgaccc catattcagc aacaaatcga ttggtaacat atggacaatg gcataggcaa
    12841 cgctaagtcc gcccaataac acgccaaaaa agttaaaaaa tgccgccatc accacagcaa
    12901 gttgtggctg catggcacga gtataaataa cggttgccac cgcattcgcc gtgtcatgaa
    12961 aaccattgat tgcttcgtag aacaacacaa atgccagagc aagtaataac aaaagccccg
    13021 tatatatatc aaggccaaca aataaattta gcatatatta gattacgcca ttttgaattt
    13081 acgaacggac gcattatcag tgactttaac ggcatgggca aagtgaaata tcatttttta
    13141 acgataaaaa gagtgttatt tcttgtgtat cagacaataa gcattcattc aaaaccttta
    13201 gaaataatca aaacacaggt attccactgg ttaatatatt cacatatgaa atgaataaat
    13261 attggaatat ataaatattg aatattttga ttaatccccg tactaattat tcttcataat
    13321 cagtacgggg ttgctacaac atgtattact ttttcaccac aattaacggt tcaatatcac
    13381 tctctttttt gatgaccagt gattcatcac cgcgcaaaca cgtcccaccg tagttgccgc
    13441 caacggtgaa ggtacatacc tgaatgtatt taccgtccac tttcggcaaa caccacagtt
    13501 gctgatagat gtttttctgc tcggcaaatt taccgctggt tttgtccagc acctcttcat
    13561 gatggctgac caggtcgata ttgctaccac agcgaccggc gatcggtttc actgcgtaac
    13621 ctgttttcac caattcatca ttaacagtga aatcggtatc cagcaggtaa cgatggtgcg
    13681 ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc ggggatcacc gtccacagcg
    13741 gctcaaagac cagcacttcc gggcgcagca atacgtcgat aagacgcact tcgttttgcg
    13801 gatgaccggt acggattggc accgcagcaa actcacggtc gctaacttca cgaatctgat
    13861 caaacgcggt ttcccacgcc caggttttcc acacgcagtt aaccagtcgc ccttccccat
    13921 caatcagttg cccggcagca tcccagccca gttcatccag cccgcgcaag atacgcgttt
    13981 caaagcccgc ctggtgcagc gcctgctcca taaactgcgc gtgatagttt tcctcgatat
    14041 ctttgtcctg catgatatgg acaaacggac gtgcacgact gtgtttccag gcaccagcca
    14101 gttcgttaat cagcccttcc gccggattga agccgttgcc tttatagccc tgctccgccc
    14161 aacgttcgag gatcaagccc gcttcggtat gacaggaggc ggagtcggcg ttgtattcgt
    14221 aaaccttcag gccacgctca tccatgcaga aatccatacg accagtgatc atatggtgac
    14281 ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat tttcggaatg tcgaacagcg
    14341 ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg aagatacatc agatgcagct
    14401 cgttggtggc tttaattagc tcctgctcgg cactctcggt aatggtgtag taatgatacg
    14461 gatcctgatt gatcacctga ccgttggcct gcacataggc gttttgtagc ggatcttttt
    14521 catccagcca tttaccgtca aactggcctt tgttttccag acgcgctcca ctgattttca
    14581 gcagctcgcc tgcaatttcc ggctgcggta agctgtattc agtatcttcc gtctggatca
    14641 tccagcccag aatggtggtg tcatcaaaag tgtctttaag ggtatagcag ccgttttcga
    14701 ccaccatctc cagctcgcgc gtccactgtt gcccttgcgg caacggggaa tgaatcacgt
    14761 tctgttccgc aatacggact ttgttgccat gcaattgggt aatgatggcg acatggccag
    14821 tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc acccgcgacc ggcgcacgcg
    14881 gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc attaaccact tctcgcagga
    14941 agcgcagcga gaaaatctcc cacgccatac ccacgtcagt aaagaccaca ccgtaattca
    15001 gaaagagaaa acggcgagca aattcaacgc attgccactt gtggcccata tattcgtcgt
    15061 cgatatagct acggaatacg gcgtcatctt cgtattcctg cggatcgaga gaactgtaat
    15121 ctgaagagta gattgctacc ccacctgggg cgtagcccaa taatgtcccg aacggggcat
    15181 cctggctggt cgttcctttg ctcatcactt tacctttaac aatacaacct aagcagttgg
    15241 cgtaattgtt gtgctctgat tacctgctca gcgacattaa ccggacagag gtcagactaa
    15301 tcatacacct catcgcagcg gctggcgcaa agggttaaaa aattcacatt ctgtacagca
    15361 agtgacctgc tacactgctt caacactacc actcagaagg caactcacta tgacagacaa
    15421 tacttatcag cccgcgaaag tctggacgtg ggataaatcc gctggcggcg cgttcgccaa
    15481 tatcaatcgc ccgttttctg gtccgacgca tgaaaaaacg ctgcccgttg gcaaacatcc
    15541 attgcaactt tattcgctgg gaacgccgaa cggtcagaaa gtaacgatta tgcttgagga
    15601 gctactggcg ctgggcgtta ctggtgcaga gtacgacgcc tggctgattc gtattggcga
    15661 tggcgatcaa ttctccagcg gctttgtcga agtgaaccca aactcgaaga tcccggcgct
    15721 gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa tctggttcga tcctgcttta
    15781 tctggcggag aaatttggct acttcctgcc gcaggatctg gcaaaacgga ctgaaacgtt
    15841 gaactggctg ttctggttac agggtgccgc gccgttcctc ggcggtggtt ttggtcactt
    15901 ttaccattac gcgccggtga aaattgagta cgccatcaac cgctttacca tggaagccaa
    15961 gcgcctgctc gacgtgctgg ataagcaact ggcgcagcat aagtttgttg cgggcgatga
    16021 gtacaccatt gcggatatgg cgatttggcc gtggtttggc aacgtggtgc tcggcggcgt
    16081 gtatgatgcc gcagagtttc tggatgcggg aagttataaa catgtgcaac gctgggcgaa
    16141 agaagtgggc gaacgtccgg cagtgaaacg tgggcgtatt gttaaccgca ccaacgggcc
    16201 gctgaacgag cagttgcatg agcgccatga cgccagtgat tttgagacaa atacagaaga
    16261 taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc catccaggcc ggataaggcg
    16321 ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg acgctgccgc gtcttatcag
    16381 gcctacaaaa gctaatcatc acgcgctggt aatgtcgtaa tccatttggc gcagtgcgtc
    16441 taatgtggct ttggcttcat cttcgtcgat gatgctcatg gcaaatccga cgtgtaccag
    16501 cacccactgg cccagcagat cggcagggtt accttcgcaa atcagggcga tattcacgtc
    16561 gcgcttgata ccacatactt caacctgcgc aagctggtga atatcttcac cgacagccag
    16621 cacctggcct ggaacgccaa tacacattgt taactccggt tatttcgatt gccggacaaa
    16681 cgcgaaaaag aacctttagc gggtgttccc tctcacccta accctctccc cggaggggcg
    16741 aggggaccgt ccgtgcgctt tttaggcgtt gtcctcagtc ttattcaact tcaatacttt
    16801 tgacgatcag cgaatcgccg gtatcgacac gcaaccgctc gccgtgacag agcggacact
    16861 gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc ccagcaccag gcctgggcgg
    16921 gtttatagac gatatgtaaa tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa
    16981 aactaaaacg gacggcgctc tcctcaacgc aggagagcgc gccaatttcc agccacacgg
    17041 cggtgacgcg cttaacatcg tgctgctccg cctgccgttg gataatttca acggcgctct
    17101 ggcaaagcga caactcatgc attttcgccc ctcctgcggc caaacagcag ggcgcgacgc
    17161 cctgcgtgtg gtacatccgg attggtgact ggcaacgaaa ggatcattcg tgcgcagtca
    17221 tccgtcaggc gttggccttc ttcaatcgac atgctgtgcg aaagcggcga catcagcgaa
    17281 caggagaggt attgcgaaac accgtccagt tcgccaacgg taaaggtcat ggtgccatac
    17341 ggcagttgca gaccaatttt ttcactcact ttgcgcagcg gccagagttg atccgggccg
    17401 gggaagataa ctgcactcag catccacggg gtgatcacac accccgtcca ctgaccttcg
    17461 aacagcgtaa aatcagaaac atacaccggc attgaaggat gcagaaaaga gagatcgtgc
    17521 atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg
    17581 aaacctgcta tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgcctgattc
    17641 gcgcagcgca gccagaacct gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt
    17701 taagccgatg tgtggctcca gcgattccgg gatcacgccg accagggtca gctttttcgg
    17761 aaactcaccg gtgaagcgca gggccgacaa tacgtcggcc aggccaagct gatgcggaga
    17821 gattttgttg gtaaacaacg ccggaacttc ttcatcccgc aggatcatca tcgttcccgg
    17881 agtgttcttt ttcgacacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc
    17941 gccaagcagc tccattcccg ccgtgccgcc atcgaggatc tcaacataat ccggcagaat
    18001 gtatcgttgc tctaacgctt cgacaatccg cacgccgatg gcttcatcgg tcagcaaaat
    18061 attgccgacc cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg
    18121 ccgtcggcat ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta
    18181 cgcaccactt ccagcggttt attcggatcg gcaaccggtg tacccaccag cgactgctcg
    18241 taaggaccga cgtcatcatt gaagttacgc ggaccagagt tccaggttga tggaacaacc
    18301 gcctggtagt tgctgatgat accgtcttta ataaccatcc agtgagagag cataccgcgc
    18361 ggcgcttcga ggaagccaac acctttgaac tcacccgttg ccggaatgtt cggcttcaca
    18421 aaggtggtgt gatcgccttt gccgatattg gtgatcagtg cactgtattg gttttgcagg
    18481 atatcctgca attcacagca gtgaacggta cgaccaataa tacggcccag cgtggagtgc
    18541 agctgtgcca cttccagcgt gttgccagtc agtttctgat aaatcgcaac gatttcattc
    18601 agtttgttct gggtagattc gcgacctgcc gccagtttca ccagcatatt agccagcgga
    18661 cccacttcta ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttcccgtcg
    18721 tcagaccaac catcataagc cggaatggtg gtaccttccc acggcgcctg tggcgcttcg
    18781 tctttatacc aggagtgctt cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa
    18841 tgagaagtga tcggacgata cgaggacaga tccgcattct caatgtagcc tcccgggaac
    18901 aggaagctgc cgtttttgct gtcggtcggg aattccggca cgctcaggta gttcaccgca
    18961 cctttaccgc gcgtcagcca ttccgggtag aacgcggcga taaccgcggt atcaacctta
    19021 taaacctgct caacaaagtc gctcagtttg tcgatgaaag acttgatgta catcaggcgc
    19081 tcaaggttca acacgcccaa cccgtcgagg ttgattgggt tcgcgacacc acccactgcc
    19141 aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc
    19201 tggcactcca gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt
    19261 ttcatcgccg ggtgacccca gtagccattg gcgaaaatac ccaactgacc gctggcaacc
    19321 agatctttga tcttgttctg aactttagtg aactcttccg gactgttcag atgccaggtc
    19381 gaaacgcctt tcagcatttc ggaggctttg gttgggtcag cttgcagtgc agaagtgatg
    19441 tccacccagt ccagcgccga aagctgatag aaatgaacga tatggtcatg cgtggtgtgc
    19501 gcagccagaa tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagcgca
    19561 ctttctgccg cacgaacgga agacagcgcg tgagtggtag tacatacgcc acagatacgt
    19621 tgcacaatca tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc
    19681 cacatggtac cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg
    19741 cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc
    19801 tcgcctcatg acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa
    19861 tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt
    19921 cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa tgccaccaac gaataggtca
    19981 gacgccaggt tgcacaacct aacagtgcgc tcagtgctga caggaacagc atgcgggaat
    20041 cattacgcag cttcgccaca cgcagaacga ccagcgggaa gagcatcagc aggacttcaa
    20101 tccagaacat cacggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga
    20161 taagctcgcc aaagcgcagc ccgatgaaaa tcgccagcaa cacactgatg gtgttggtca
    20221 gcttgacgaa caggctcttt tcatccggac cgttgccacg cagacccgcc tgcaccagtg
    20281 aaccttcaaa gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg
    20341 gcaacatttc atagctctgc cagagcggat gcaccttgta gcccgccgag atcatcagcg
    20401 accccattga agactggtgc atggtcggca gcagcgcacc gagcgcgatg atgaagaaca
    20461 tcaccttgtt cagacgttgt agcgacacct tccagcccag acgttcaaac agtgccggag
    20521 caaactccag agccatcacg ccgatgtaga tggtcataca gaccgccgtc tcgaacagta
    20581 ccgagttcac gttgaagtga cccggaatgt agaagtacgg caggctccag tagcgaccca
    20641 cgtcgatagt gatcgacaag ccacccagtg agtaaccaaa cagactcgcc aacagcgccg
    20701 gacgcaccag cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc
    20761 agccgccaca ggcaaagccg gtgccaatca gcaggtcaaa cgcgatccac acgccccacg
    20821 ggaagccgcc gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca
    20881 ggagcataca gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc
    20941 cgcccagcgg ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg
    21001 gtgttgcgac gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct
    21061 ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc
    21121 ggcaggtcga gattttcata aggcacaccc gtcagtacca gaacctgagt accgccgccc
    21181 tctttctcgc cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca
    21241 gatttcagcg tctgacgcgg atagtggtat tcgctgccag gcttcagcgc cagacgtttt
    21301 ttcgcctccg ccatcagctc ttcacgcgta ccgaaaatca ccgcgcccgc cgggcacact
    21361 tctacgcagc caggcagacc gcctttatcg agacgttcca cacctttctg gttgcacagc
    21421 tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt acttcggcac gttgtacgga
    21481 caggcgacca tgcagtaacg gcaaccggtg cacacgtctt tgtcgtaatg gacaatgccg
    21541 gttttcggat cttttttcag tgcagagacc gggcacacag agacacagtt cggatcgacg
    21601 cagtgcatac actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc
    21661 cctgtgccgc tggtccacac ctgaatgatg ttattggtat acggcgacag tttgtcgttg
    21721 ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga agttgatatc ctgacacttg
    21781 gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt acaacatccc cagcgatccc
    21841 ggaattggcg ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc
    21901 aatgccccgc aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg
    21961 agtcagcgtt atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac
    22021 cggcaaccag cccaaccaca ccgccgagca aaccaatagc gcctgcagag acgttgccgc
    22081 cctctttagc gttaacatcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa
    22141 gctgatggat gcctttatgg aagccgatac ctttttcgtt acagccatag cacgggtgac
    22201 caatcgccac cggccacaca ccgccaacat cgcagaattg cagcgttgag cagttgccgt
    22261 aagtttctgg ccctttacag ccgaggtggt acaggcacca gccttcgcgg tggccttcat
    22321 caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt
    22381 gaatcagacg gccataggcg aaggtcggac ggtttttgtc atccagtttc ggcggtttgc
    22441 cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg
    22501 gaatattgat aacggttttg cctggcagaa cttcttgcaa gctgactgcg ccagttgggt
    22561 taactccagc tgcggcaaca ccgccccacg cggagcagga accaatagcg ataatggctg
    22621 ctgagccttc cgccgctttg cggatgtgat ccacaatcgg ctcaccggca accatgcaat
    22681 aaataccgtt atcttttaat gggatggaac catccaccac taacacatac tgccctttgt
    22741 acttctcgag agcgttatgt ttgttctctt cgacctgatg accgaaggcg gcggaaagca
    22801 cttcgtgata ttccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg
    22861 cacgaagcag agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg
    22921 gacgctgcgg gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca
    22981 tggtggcggc taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat
    23041 ggatgagggt gttatctcca gtcatttata gttattccgt tgcgaggacc tggcatatat
    23101 tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat
    23161 ggttatcgat atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt
    23221 aattaattaa agcaagataa tacgtatgtt tgatcaattt tcgaggcaat aaccggccat
    23281 aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt
    23341 atccagtcca tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc
    23401 ggtcttttcg tacatccggt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca
    23461 gaattttgtc acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc
    23521 tttaccaaaa tggttattca tcgttcacct cattcgttat atatcgccat atatagcgat
    23581 attttaggca aagaacgact tttgcgcacg ctattttttc atacttgttt aagacgtaat
    23641 cccgtaagag ataaacctat caggagagat atttatcgaa ccatttcagt gtcctttgcc
    23701 aggcaagatc ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat
    23761 taacccccgg atagatataa gcctcataaa ccttattatt gtctttcaac gccgcctcgt
    23821 aaggaggcca gccctcgttg attcgggtgt ccagttccgc gaagtggagt agtaaaggcg
    23881 cttcaatctt cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg
    23941 ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc accgccatag caaaatccgg
    24001 taatacccac tttacctgtc gcttgcggat agcgttgcat aaactcaatt gcggcaaaga
    24061 aatcattcat cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat
    24121 ttcccggata acctccaacg gaacttaatc cgtcaggtgc cagggcgata taccccgcct
    24181 tcgccactcg ccgtgccaca tcttcgatat acggattcag tccacgattc tcatgcacca
    24241 cccccacggc tggcgttttg ccgctcattt ttgcgggttt caccagataa ccccgtacct
    24301 cgccgtgacc atttggcgaa ggatacgtga tgtactcagc aacaatctcc gggtcggtaa
    24361 actctacctg agtcgccagc gcataatttg gcttgagcaa atcaaacaac gctaatgccg
    24421 tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gaaattttcc
    24481 cgtgaacgta atagtcgtag taatccaaca actcttgtgg gaaatctttg gcggttaaac
    24541 gcggcatcgt tgcattcctc aactggtgtt tttttaagca aagcataagc gcgtgttttt
    24601 gcccagtttt tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa
    24661 ctagaaacat tgtttcgaaa ttgaacggag gaaaggagag gtcatggtct ggttagcgaa
    24721 tcccgaacgt tacgggcaga tgcagtaccg ctattgcgga aaaagtggtt tacgcctgcc
    24781 cgcattatcg ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg
    24841 tgcaatcctg cgtaaagcgt ttgatttagg cattacgcac tttgatttag ccaacaatta
    24901 cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc ctgctgcgcg aggattttgc
    24961 tgcttatcgc gatgaactga ttatctctac caaggctggc tacgatatgt ggcccggccc
    25021 gtacggctct ggcggttcac gtaaatacct gctcgccagc ctcgaccaaa gcctgaagcg
    25081 tatggggctt gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat
    25141 ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg
    25201 gatctcctct tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa
    25261 aattccgctt ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag
    25321 cggcctgctg gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc
    25381 tcagggattg ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgcatcg
    25441 tgaagggaat aaagttcgtg gtctgacgcc gaaaatgctt accgaagcca acctcaacag
    25501 cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt
    25561 aagctggttg ctgaaagatg atcgcgtgac gtcggtattg attggtgcca gccgcgcgga
    25621 gcaactagag gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc
    25681 gcagattgat cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa
    25741 atgacctgtt aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag
    25801 tcagtcggtc aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga
    25861 cgtaccacat cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaagt
    25921 gaatggaaga aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca
    25981 ttttccccag ccacttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat
    26041 tctcataacc ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca
    26101 gcgacagcaa cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt
    26161 gaataatctc ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt
    26221 acacgggggc aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac
    26281 attcctgtcg tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa
    26341 cagcaaatca acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc
    26401 tggttccctt atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga
    26461 gaaaaacgat gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca
    26521 aacagaaaca accgacgcca ggcatccagg cgaagatgac accgataccg gattgcggcg
    26581 agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg
    26641 attccggaat aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga
    26701 tcagttatct tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat
    26761 gcggacgcaa agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc
    26821 tggttcacga agcgcacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga
    26881 aacaggttgc cattccggat attgcagacc tcaccagcga acagtttcaa aagacctttg
    26941 ccattaacgt tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag
    27001 gtgcaagtgt catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg
    27061 actatgcggc tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg
    27121 cggagaaagg tattcgggtg aatattgtcg cgccaggccc tatctggacg gcactgcaaa
    27181 tttccagcgg acaaacgcag gataagatcc cgcagtttgg tcagaaaacg ccgatgaagc
    27241 gtgcggggca accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga
    27301 gctacgtcac cgcagaagtg cacggcgtgt gcggcggcga gcatttaggt taaaaaaatg
    27361 cccggttgtg aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa
    27421 acgccttatc cgcctacaaa cgcatgaaga ttcaacatgt tgcaatagat tttgtaggcc
    27481 tgataagcgt agcgcatcag gcattgagca cctaatgccg gatgcgtcgt gaacgcctta
    27541 tccggcctac aaacgcacga agattcaaca tgttgcaata gattttgtag gcctgatgag
    27601 cgtagcgcat caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc
    27661 tatcttcagg taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc
    27721 gacggttttg tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt
    27781 caacgccgta atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc
    27841 tgccttcacc gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc
    27901 aggcaagttc accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag
    27961 cagaaccaac atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc
    28021 gtcgaggttt tcgttaagat gcattgccat cggacattat cctgcgcgta atttttgtgc
    28081 gacacgaacc ggatgcgcag cggcgctggc ttccagatcc aggtcacggc tttgcagcaa
    28141 caatacctgc gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa
    28201 tacgttatag ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag
    28261 agcttctgcg atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc
    28321 aataaagctg ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc
    28381 gccgatggtt gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacccg
    28441 acgctccaga cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga
    28501 cagttccagc tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa
    28561 atcagcggcg atatcgttgg cctggtttaa ggaacgtgct tcagccagca gttgctgctc
    28621 gcgcttaagg cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca
    28681 ggtgactaca gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgta
    28741 ctgatacata ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct
    28801 ccaggacgca agtcacaaaa tctgcgcata ataatatcaa aacgacgtcg aattgatagt
    28861 cgttctcatt actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca
    28921 gtaaaaaagt caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg
    28981 atgcgcccgc gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg
    29041 acatggcgga caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca
    29101 ctcttggcgc ggtaaatagc gtgattcagc gcgcttcttc gctggtattt gagagtatgg
    29161 aagccaaaaa gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg
    29221 gaacgttaac ccatttttcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct
    29281 gcgcgctatt tccctgcggg gcggcagcgg ttgctaattc gattcttgct tttgtcgaac
    29341 agggcgatca tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca
    29401 aaatcctcag caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata
    29461 tcgttaagca tctgcagcca aacactaaaa tcgtgtttct ggaatcgccg ggttccatca
    29521 ctatggaagt ccacgacgtt ccggcgattg ttgccgccgt gcgcagtgtg gtgccggatg
    29581 ccatcattat gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg
    29641 gcatcgatgt ttctattcag gccgccacca aatatctggt tgggcattca gatgcgatga
    29701 ttggcactgc cgtgtgcaat gcccgttgct gggagcagct acgggaaaat gcctatctga
    29761 tgggccagat ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag
    29821 gtgtgcgttt gcgtcaacat catgaaagca gtctgaaagt ggccgaatgg ctggcagaac
    29881 atccgcaagt tgcgcgagtt aaccaccctg ccctgcctgg cagtaaagga cacgaattct
    29941 ggaaacgaga ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca
    30001 atgatgaaga gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt
    30061 ggggcgggta tgaatcgttg atcctggcaa atcaaccaga acatatcgcc gccattcgcc
    30121 cacaaggcga gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg
    30181 tcgacgatct gattgccgat ctggacgccg gttttgcacg gattgtataa cattgccact
    30241 tttggacaat tttgcagaca ttttattgtg aaaagtctta aattgttgcg tccgggatca
    30301 aggcgtcccg gacgattcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca
    30361 ggaaagtcca tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc
    30421 gcgctggcgg atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt
    30481 ttagaaaacg gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca
    30541 ggcgcattga ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc
    30601 gcagcaagtc tgggctgctg gctaagttat attcagggga gctggttagg gaataccaaa
    30661 acggtgaaag gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg
    30721 tttgaccgcc acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg
    30781 ctgctgccaa ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac
    30841 tggttaagtg gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg
    30901 attccgttcg ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt
    30961 gccttgttaa ccgctggctt gttaggcacg ctgtttgtgg tgattaaaaa aaaatactgt
    31021 aacgcctgac gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg
    31081 catcgcccgc attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc
    31141 acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc gctgctgcgc tggccttcat
    31201 gccgtcatgg atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa
    31261 cggcgaggtg ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc
    31321 ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat ttattctcaa ttcgtttcag
    31381 cacgcggcta atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc
    31441 gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg
    31501 cgcatccagt ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc
    31561 tgataacgtg gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc
    31621 gccaatgtcc atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc
    31681 cagcggcacc tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag
    31741 caggtattca ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc
    31801 ggaaaagaga attatgatgc caggctcgta catcaccggt gtacgtgcga aaggcgtctc
    31861 gccatacaac aaacgcacat cgggcaacag ttctgacaaa ctattttctt tatttttcag
    31921 tttattaact ttatccgcca gcaagcgaca aatctcttca cgtttcatat cgcgtaattt
    31981 cttaggaata atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg
    32041 gagaaatagg caagacattg gcagaaatga gcattgagag ccagggcgct ggcgatcaca
    32101 atgaaaaaca tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt
    32161 aatgaacaac tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc
    32221 tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg attacctacg gtggcggcag
    32281 cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct
    32341 ggaatttggc ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact
    32401 ggttcgcgaa cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg
    32461 caccaaattt atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct
    32521 gcaaacgggc ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc
    32581 agcaaccggt tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa
    32641 gcaggcgttc cattctgcac atgttcagcc ggtatttgcc gtgctcgatc cggtttatac
    32701 ctacaccctg ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt
    32761 ggaacagtat gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat
    32821 tttgctgacg ctaatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt
    32881 gcgcgccaac gtcatgtggg cggcaactca ggcgctgaac ggtttgattg gcactggcgt
    32941 accgcaggac tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga
    33001 tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa
    33061 gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgacga
    33121 tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc
    33181 gacccacctc tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact
    33241 ggaagagcac ggcatgaccc aactgggcga aaatcatgac attacgttgg atgtcagccg
    33301 ccgtatatac gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt
    33361 tcgtccagac ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag
    33421 gaggaacgta tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag
    33481 ctgggactgg gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg
    33541 ttagaagtgg gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc
    33601 ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg
    33661 tggaacgacg accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag
    33721 cttgattata tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc
    33781 gaagcatgga aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg
    33841 tgcaacttcc agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg
    33901 ataaaccaga tcgaacttca tccgctgatg caacaacgcc agctacacgc ctggaacgcg
    33961 acacacaaaa tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc
    34021 gatcagaaag tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt
    34081 atccgctggc atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt
    34141 attgccgaaa actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcaaaatt
    34201 gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacgt
    34261 gcaaattctc ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc
    34321 cctcgcgtcc cgcccgttgt tactcttcct tgttcaggaa tgccaaatat aaggacatca
    34381 tcatgcagag ccggaagctc ttaaaagaac aactcatcta tatccgggat aaacgcaacg
    34441 gagaggtgaa aaacagatga aaataatact tctgttttta gcagccctgg caagttttac
    34501 cgtacacgcg cagcctcctt cacagaccgt agaacaaaca gtccggcaga tttatcagaa
    34561 ctataaatca gatgccagta cgccttattt cggtgaaacc ggagagcggg cgataacttc
    34621 agcgcgtatt caacaggcgc ttaccctgaa cgacaatctt acactaccgg gcaatattgg
    34681 ctggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgttagaaag
    34741 cgtagcgata acccaaactg acgtcgatca tgccgatgct gttgtgcgct ttcgtatctt
    34801 taaagatgat aaagaaaaga ccacacagac actgaaaatg gtggcggaaa atggtcgttg
    34861 ggtcattgat gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    34921 tgaaaaaacg ctggccgctt tagcttcgtt gcagaaagaa cagccagaat cctttgttgc
    34981 tgaactcttt gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc
    35041 ttaccgccag gcggttaatg ccttctataa aaccaccttc aagacggcca ataatcccga
    35101 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    35161 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    35221 cattcatgtt cgttttacgc tgaccaatgg caacaatgaa gagcaagagc tggttttaca
    35281 gcggcgcgaa ggtaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    35341 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa
    35401 ttagccggaa caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct
    35461 gttgttctgg caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcaactg
    35521 gcgagcaatg tacaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca
    35581 ctacgcccac aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat
    35641 gcacaggact attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat
    35701 ccccggcagc atgtcagttg ccggggattt tttttaacgt ccaaccgccg ctttagggcg
    35761 tttcttcgca ccagcattca ccggacgaga ttgcgtagac gacgcttttt ttgccgtagc
    35821 aggcgtctga cgctgagtcg ccatcggcgt atgtttagtc aacgccggac gggtattgcg
    35881 gttctggcga cgagcttcac gcatctcttc aatggttggc gcaggcacta agcaatcgcg
    35941 acggctgcca atcagatgct ttttgcccat cgcttccagc gcctggcgga ttaacggcca
    36001 gtttgccgga tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgtt tgtcgccctt
    36061 cggtacgaag acgccttcgc tcttataacc aatcttcgcc agcggttttt tcccggtgta
    36121 atacatggtg gttgagttcg ccagcggcga tgggtagaag ttctgtacct ggtcgagacg
    36181 gaaacgatgc tttttcagcc acagcgccaa attcaccata tcttcatcac gcgtaccggg
    36241 gtgcgcagag atgaaatacg ggatcagata ctgctcttta cctgcctgtt tcgagtaggt
    36301 atcgaacagc tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgacaa
    36361 cggcccttct tcggtatgtt ccggagcaat cttcagataa ccgccgacgt gatgggtcgc
    36421 caactctttg atatagcgcg gatcttccac ggcgatgtca taacgtacgc ctgaggcgat
    36481 aaggattttt ttaatgcctt tcagatcacg cgcacggcga tagaggttga tcgtcggttc
    36541 gtggttagtg tccatgtgcg gacaaatatc cggataaacg cacgacaaac ggcgacaagt
    36601 ttgttcagcg cgtggcgatt tgcagcgcaa catatacatg ttggcagttg gcccaccgag
    36661 atcggaaatc acgcccgtaa aacctggaac ggtgtcgcgg atcgcttcga tctcattaat
    36721 gatcgaatct tccgaacggc tctgaataat gcgcccttcg tgctcggtaa tagaacagaa
    36781 agaacagccg ccaaagcagc cgcgcataat gttgaccgaa aaacggatca tttcgtaagc
    36841 cggaatacgg gcattgccat aggccggatg tggcacgcgc ttgtacggca gcgcaaaaac
    36901 gctgtccatc tcttcggtag aaagcgggat agcaggcggg ttgatccaca catagcggtc
    36961 accgtgtttt tgcatcaatg cgcgggcgca gcctgggttg gtttcgtggt gcagaatgcg
    37021 cgaagcatgg gcgtacagca ctttatcgcc cttcactttc tcgaaagaag gcagcaatac
    37081 gtaggttttt tcccacggtt tctggcgcgg tggctgcacg attacagctt tcgcttcctg
    37141 ctttttcggc gcaacaggtt tgttatccgc acacggcaaa tcttcgccat acggatgcgg
    37201 gattgggtcg attttccctg gggtatcaag acgggtggaa tccacaccgc tccagccagg
    37261 cagcgcctct tttacgataa ttgcggtatt acgcacatcg cggatttcac taatcggctc
    37321 gcccatcgcc agacgatgcg ctacttccac cagcggacgt tcaccgttac caaacatcag
    37381 catgtcggct ttcgaatcta ccagaacgga acggcgcacg gtatcggacc agtaatcata
    37441 atgcgcggta cggcgcaggc tagcctcaat accgccgagg atcaccggca catctttcca
    37501 cgcctcttta caacgctggg tataaaccag cgtggcgcga tccggtcgct tacccgcgac
    37561 attatctggc gtgtaggcat catcatgacg taaacggcga tcggcggtat agcggttgat
    37621 catcgaatcc atgttgccag cagtaacacc aaaaaacaga ttcggtttac ccagacgcat
    37681 aaagtcgtct ttgctgctcc agtccggctg ggcgatgatc ccgacgcgaa agccctgcgc
    37741 ttccagcata cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc
    37801 agtaaccaaa atgatgtcgc agctatccca gccaagttga tccatctctt cacgagacat
    37861 cggcaaaaac ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc
    37921 gcgatccggt tggatcaggg agatagagct cattttgctt ccagaaatga taaaaaaata
    37981 atcaaaggcc ggggattata agccggaacg aaagagaaat cgaaaggtat tccatactcg
    38041 ccctcctcgg gcgagtatga agattacggt accggattga ccaacagttg cccaatcgag
    38101 ccacggtccg ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa
    38161 ggctgaccga aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag
    38221 cctctgtctt ccgggaacgg catcgcgcca ttcacgttac ggatctggaa cattacgcct
    38281 tcaatatgga acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca cgttccttgc
    38341 tgcgcggtga catcaatacg gttgacgtcc cacagctgtc cattaatacc cgggtcatca
    38401 cccagactga tatcgcgact gcgaattggc gaaccagcca tgatttcagt tggcagcaag
    38461 cgcatcggaa gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc
    38521 aaggtagaaa ccagaatact ggatggctca aagaagccgc gaatacgatc aacaatgctc
    38581 gccgcttcac cacaggtgat cgacacttca tcgccgttgc tcatatccac cagaatctcg
    38641 cggcgctcgc ccggtgccag cgaaagttgc ttcaccgata caggagcagg caggaatccc
    38701 tgatcgccag aaatcacatg taacgggcga ccatcgctca tctgtagttg atagcgacga
    38761 gagttcgacg cgttcagcag tcgcaagcgc acccagccac gcgagacttc aacgtacggg
    38821 ctttgtacac cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgttg
    38881 tattctggcg taccaaagtt atccagccgt ttatcctgga taatgaccgg aaaatcatcc
    38941 acaccataat ggttggggat aggcagcgac ttgctgactt catcttccac cagccacatt
    39001 ccggcaaggc cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac
    39061 agagtagctg cgttctggcg aataggcagt acgggtgccc agtcagcgtt tggcgacatc
    39121 atccgtgccg gaccgcccat cagcgggcct ggtacctgta gcccggccac cgtcattgag
    39181 acattttctg tcaggcggtt gctgtaaata agcttaacat cgtcgtcctt ccagacgcgg
    39241 atagtcggcc ccaggtaacg accattgatt ccccagaccg acgcgcgtgt ccctggcgta
    39301 aatgaccagt gcgcacgttg tacagtcata aacagcggtt gcccacggcg agattcaagt
    39361 agcggcggaa cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg
    39421 cctgcacaaa gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt
    39481 gctccatgta aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca
    39541 gattgccgac ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg
    39601 cgattttttg ttccattatc gaacgacaat gggcagccag ctcacgaacc tgatctttgc
    39661 catactgact gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgcagtc
    39721 gattaagatt aatcttattc gaagttgtag agacgcacac gggaataatc gggacgcccg
    39781 ccgcaattgc cgcgtgaaat gctccagtct tgaacggtag caggccgcga ccacggctgc
    39841 gggttccttc cgggaacatc cagatggaaa tacggcgttt tttgaaatga ttcacgactt
    39901 ccgcaatggt gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta
    39961 accagtacaa ctgcccgaag aaggggatcc acagcaagct ctttttacct accgtcaccg
    40021 tcggcggttg cacaatgttc gatgctgtca ccatgtcata gttgttctgg tggttagcga
    40081 tatagatagc attgccgtag ctttcagcat ctgcaggttt gcggcactca actttcaggc
    40141 cgaacagtgg cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac
    40201 gcgggctgaa aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg
    40261 taataataag acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt
    40321 gtacgtcacc tgaaatcagg ttagggcatt gttaaaagcc gctctctgga aaagcgtatt
    40381 gttaatcgca acgcatgaat aaacaacggt ttcaagggaa tttttatcgt ttccgggggt
    40441 tgaatacgct gccggatgac gacttaacgt ttcatccggc gttccttgct agcgggagga
    40501 aacagcgccc tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga
    40561 gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg
    40621 ttcgccagtg actttctgta gctcttccgg acgcagttta attttgcgtt tcccaacatg
    40681 aatggtcagc gtgctttgcg gcggcagaac gtacagttgc gccagaccat cctcgccacg
    40741 cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg
    40801 cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc
    40861 ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa
    40921 agccttaccc gcacggttac gcgctaccag atcgttaaag gtgcagacga aaccgtaacc
    40981 cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt
    41041 cgccccaggc ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg
    41101 cagcgtaatc gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg
    41161 gttactctta cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg
    41221 cgcgtcgata tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt
    41281 gacaggttca gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg
    41341 caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag
    41401 caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc
    41461 cagctcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt
    41521 cagttcgagg atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg
    41581 tttcggttca tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac
    41641 cagcaaacct tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg
    41701 gcggcgcacg gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt
    41761 caccgccgga cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag
    41821 atcggtggta gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg
    41881 cacaatcacc agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat
    41941 cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac
    42001 ctgatgcggt aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat
    42061 acgcactgaa ccacgtccgt tctcgtagat tttacgaatc tcggcgcgcg aagtgataat
    42121 ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtagt
    42181 tttcggctgg tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg
    42241 aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag
    42301 acgggcaggt agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc
    42361 cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt
    42421 gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc
    42481 aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc
    42541 gctatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt
    42601 aaatttggcg ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac
    42661 aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta
    42721 gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat
    42781 taattctgat tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact
    42841 acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc
    42901 cgacggcgat aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg
    42961 gcatagaggg cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc
    43021 gggcgattcg agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc
    43081 gactgtaagt atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc
    43141 tgccataagt gcggcttgct catggatatc cccgagacgg cttacggcgc accggataat
    43201 aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc
    43261 ctgtgacatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt
    43321 ccccatcacg ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg
    43381 gcaagattgg tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc
    43441 agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg
    43501 cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg
    43561 ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg
    43621 gcaggggcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc
    43681 tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg
    43741 tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg
    43801 tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa
    43861 ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc
    43921 gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt
    43981 gtgatcgata tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga
    44041 gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc
    44101 gctttctaca gtcgttacgc tgccgttcgg cccctgaaat cctccggcct gactttgcgt
    44161 taccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcac tgcacagggc
    44221 cattactgcg attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt
    44281 ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttaatg
    44341 ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga
    44401 aaaaatgcga attttactga tagaagatga catgctgatt ggtgacggca tcaaaactgg
    44461 ccttagtaaa atgggtttta gtgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc
    44521 gctttatagc gcaccttatg atgcggtgat cctggattta accttaccag gcatggatgg
    44581 tcgcgatatt ttgcgcgaat ggcgagagaa aggtcagcgt gagccggtac tgaacctgac
    44641 cgcgcgtgat gcgttagcgg aacgtgtaga agggctgcgt ctgggagctg acgattatct
    44701 gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaggctctga tgcgccgaac
    44761 caacggccag gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg
    44821 tatcgccacg ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga
    44881 attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta
    44941 tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg
    45001 caaactcggt agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa
    45061 atgaaattta cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg
    45121 gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc
    45181 gatgaattgt tcgacaccca actgatgctg tttgccaaac ggttaagtac gctcgatctc
    45241 aacgaaatca acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat
    45301 gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaat
    45361 gatggcgata acggagaaga tattccctat agctatcaac gggaaggttt tgctgacggg
    45421 caactggtcg gtgaagacga tccttggcgt tttgtctgga tgacctcacc tgatggcaaa
    45481 tatcgcatcg ttgttggcca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt
    45541 gccgggcaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta
    45601 ctactgggtc gtgaactcgc gccgctgaac aaactggcgc tggcactacg tatgcgtgac
    45661 cctgactcgg aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg gccactggtt
    45721 gagtcgctaa atcaactgtt cgcccgcaca catgcgatga tgattcgaga acgacgcttt
    45781 acctccgatg cagctcacga actacgtagc ccgttaacag cgctgaaagt gcaaaccgaa
    45841 gttgcgcagc tctctgacga tgatccgcag gcgcggaaaa aagcactgct ccaattacat
    45901 tccgggatcg atcgcgctac tcgtctggtt gatcaactgc tcacgctatc gcggctggac
    45961 tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct acaatcgtcg
    46021 gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat
    46081 gcccacggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg
    46141 ctggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    46201 cgcccacccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    46261 gccaagctgc atgacatgaa tgttgagttt gggaatgcgg aacaaggtgg atttgaggcg
//
LOCUS       DAJTRB010000024        45804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTRB010000024 DAJTRB010000000
VERSION     DAJTRB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.024"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            114..2267
                     /locus_tag="OO330_001424"
     CDS             114..2267
                     /locus_tag="OO330_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5882347.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OO330_001425"
     CDS             2320..4050
                     /locus_tag="OO330_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882348.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO330_001426"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OO330_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5882349.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OO330_001427"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OO330_001427"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5882350.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OO330_001428"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OO330_001428"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5882351.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO330_001429"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OO330_001429"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5882352.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OO330_001430"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OO330_001430"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5882353.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OO330_001431"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OO330_001431"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5882354.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OO330_001432"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OO330_001432"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5882355.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OO330_001433"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OO330_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5882356.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO330_001434"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OO330_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5882357.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO330_001435"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OO330_001435"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5882358.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO330_001436"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OO330_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5882359.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO330_001437"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OO330_001437"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5882360.1"
                     /translation="MNIIEANIATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO330_001438"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OO330_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5882361.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO330_001439"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OO330_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5882362.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OO330_001440"
     CDS             16308..16847
                     /locus_tag="OO330_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5882363.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OO330_001441"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OO330_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5882364.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OO330_001442"
     CDS             complement(18206..18553)
                     /locus_tag="OO330_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5882365.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO330_001443"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OO330_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5882366.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEEPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO330_001444"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OO330_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5882367.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO330_001445"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OO330_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5882368.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO330_001446"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OO330_001446"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5882369.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO330_001447"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OO330_001447"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5882370.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OO330_001448"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OO330_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO330_001449"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OO330_001449"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5882371.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO330_001450"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OO330_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5882372.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO330_001451"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OO330_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5882373.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO330_001452"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OO330_001452"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5882374.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO330_001453"
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OO330_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5882375.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OO330_001454"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OO330_001454"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5882376.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSCEQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OO330_001455"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OO330_001455"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5882377.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEKLTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OO330_001456"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OO330_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5882378.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO330_001457"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OO330_001457"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5882379.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OO330_001458"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OO330_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5882380.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OO330_001459"
     CDS             complement(40011..40466)
                     /locus_tag="OO330_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882381.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OO330_001460"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OO330_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO330_001461"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OO330_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5882382.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OO330_001462"
     CDS             complement(41309..41986)
                     /locus_tag="OO330_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5882383.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDYQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OO330_001463"
     CDS             complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OO330_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5882384.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO330_001464"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OO330_001464"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5882385.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OO330_001465"
     CDS             complement(43192..43650)
                     /locus_tag="OO330_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5882386.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OO330_001466"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OO330_001466"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5882387.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OO330_001467"
                     /gene_synonym="dgcC"
     CDS             complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OO330_001467"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5882388.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11908 a        11944 c        11205 g        10747 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgtc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttg cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa tgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gttcttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc cagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat caggtggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtt gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgagaag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgcccta gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atggtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtgccg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccaa gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat aatcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttgtt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgataa tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactat ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gcgatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca
    45721 attttcccaa attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac
    45781 tttcgtagct cacccgttag cact
//
LOCUS       DAJTRB010000025        42838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTRB010000025 DAJTRB010000000
VERSION     DAJTRB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.025"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(137..673)
                     /gene="ssb1"
                     /locus_tag="OO330_001468"
     CDS             complement(137..673)
                     /gene="ssb1"
                     /locus_tag="OO330_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5882389.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            927..3749
                     /gene="uvrA"
                     /locus_tag="OO330_001469"
     CDS             927..3749
                     /gene="uvrA"
                     /locus_tag="OO330_001469"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5882390.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3784..4140)
                     /locus_tag="OO330_001470"
     CDS             complement(3784..4140)
                     /locus_tag="OO330_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5882391.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4144..4560)
                     /locus_tag="OO330_001471"
     CDS             complement(4144..4560)
                     /locus_tag="OO330_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5882392.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4671..5384)
                     /gene="aphA"
                     /locus_tag="OO330_001472"
     CDS             complement(4671..5384)
                     /gene="aphA"
                     /locus_tag="OO330_001472"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5882393.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5786..5990
                     /locus_tag="OO330_001473"
                     /pseudo
     CDS             5786..5990
                     /locus_tag="OO330_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6493..7686)
                     /gene="tyrB"
                     /locus_tag="OO330_001474"
     CDS             complement(6493..7686)
                     /gene="tyrB"
                     /locus_tag="OO330_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5882394.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7939..9018)
                     /gene="alr"
                     /locus_tag="OO330_001475"
     CDS             complement(7939..9018)
                     /gene="alr"
                     /locus_tag="OO330_001475"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5882395.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9071..10486)
                     /gene="dnaB"
                     /locus_tag="OO330_001476"
     CDS             complement(9071..10486)
                     /gene="dnaB"
                     /locus_tag="OO330_001476"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5882396.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10569..11552
                     /locus_tag="OO330_001477"
     CDS             10569..11552
                     /locus_tag="OO330_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5882397.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQCAHEILESRATQGSSLLIP"
     gene            complement(11718..11960)
                     /gene="pspG"
                     /locus_tag="OO330_001478"
     CDS             complement(11718..11960)
                     /gene="pspG"
                     /locus_tag="OO330_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5882398.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12094..13131)
                     /gene="dusA"
                     /locus_tag="OO330_001479"
     CDS             complement(12094..13131)
                     /gene="dusA"
                     /locus_tag="OO330_001479"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5882399.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13494..14479)
                     /locus_tag="OO330_001480"
                     /pseudo
     CDS             complement(13494..14479)
                     /locus_tag="OO330_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14797..15312
                     /gene="zur"
                     /locus_tag="OO330_001481"
     CDS             14797..15312
                     /gene="zur"
                     /locus_tag="OO330_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5882400.1"
                     /translation="MGKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15354..15563)
                     /locus_tag="OO330_001482"
     CDS             complement(15354..15563)
                     /locus_tag="OO330_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5882401.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15679..17004)
                     /gene="dinF"
                     /locus_tag="OO330_001483"
     CDS             complement(15679..17004)
                     /gene="dinF"
                     /locus_tag="OO330_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5882402.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSIAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17077..17685)
                     /gene="lexA"
                     /locus_tag="OO330_001484"
     CDS             complement(17077..17685)
                     /gene="lexA"
                     /locus_tag="OO330_001484"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5882403.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17795..18163)
                     /gene="dgkA"
                     /locus_tag="OO330_001485"
     CDS             complement(17795..18163)
                     /gene="dgkA"
                     /locus_tag="OO330_001485"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5882404.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18334..20757
                     /gene="plsB"
                     /locus_tag="OO330_001486"
     CDS             18334..20757
                     /gene="plsB"
                     /locus_tag="OO330_001486"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5882405.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20912..21784)
                     /gene="ubiA"
                     /locus_tag="OO330_001487"
     CDS             complement(20912..21784)
                     /gene="ubiA"
                     /locus_tag="OO330_001487"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5882406.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21797..22294)
                     /gene="ubiC"
                     /locus_tag="OO330_001488"
     CDS             complement(21797..22294)
                     /gene="ubiC"
                     /locus_tag="OO330_001488"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5882407.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22517..24097)
                     /locus_tag="OO330_001489"
                     /pseudo
     CDS             complement(22517..24097)
                     /locus_tag="OO330_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24326..25246)
                     /gene="malM"
                     /locus_tag="OO330_001490"
     CDS             complement(24326..25246)
                     /gene="malM"
                     /locus_tag="OO330_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5882408.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSRTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25398..26738)
                     /gene="lamB"
                     /locus_tag="OO330_001491"
     CDS             complement(25398..26738)
                     /gene="lamB"
                     /locus_tag="OO330_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5882409.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26810..27925)
                     /gene="malK"
                     /locus_tag="OO330_001492"
     CDS             complement(26810..27925)
                     /gene="malK"
                     /locus_tag="OO330_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5882410.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28290..29480
                     /gene="malE"
                     /locus_tag="OO330_001493"
     CDS             28290..29480
                     /gene="malE"
                     /locus_tag="OO330_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5882411.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29634..31178
                     /gene="malF"
                     /locus_tag="OO330_001494"
     CDS             29634..31178
                     /gene="malF"
                     /locus_tag="OO330_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5882412.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31193..32083
                     /gene="malG"
                     /locus_tag="OO330_001495"
     CDS             31193..32083
                     /gene="malG"
                     /locus_tag="OO330_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5882413.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32455..33930
                     /gene="xylE"
                     /locus_tag="OO330_001496"
     CDS             32455..33930
                     /gene="xylE"
                     /locus_tag="OO330_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5882414.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33974..34384)
                     /gene="psiE"
                     /locus_tag="OO330_001497"
     CDS             complement(33974..34384)
                     /gene="psiE"
                     /locus_tag="OO330_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5882415.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34510..34653)
                     /locus_tag="OO330_001498"
     CDS             complement(34510..34653)
                     /locus_tag="OO330_001498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882416.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34654..34932
                     /locus_tag="OO330_001499"
     CDS             34654..34932
                     /locus_tag="OO330_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882417.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34979..37075)
                     /locus_tag="OO330_001500"
     CDS             complement(34979..37075)
                     /locus_tag="OO330_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5882418.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37075..37812)
                     /locus_tag="OO330_001501"
     CDS             complement(37075..37812)
                     /locus_tag="OO330_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5882419.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37809..38447)
                     /locus_tag="OO330_001502"
     CDS             complement(37809..38447)
                     /locus_tag="OO330_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5882420.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38561..38803)
                     /locus_tag="OO330_001503"
     CDS             complement(38561..38803)
                     /locus_tag="OO330_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882421.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            complement(39265..40914)
                     /gene="pgi"
                     /locus_tag="OO330_001504"
     CDS             complement(39265..40914)
                     /gene="pgi"
                     /locus_tag="OO330_001504"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5882422.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKIFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41439..42788
                     /gene="lysC"
                     /locus_tag="OO330_001505"
     CDS             41439..42788
                     /gene="lysC"
                     /locus_tag="OO330_001505"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5882423.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10661 a        11022 c        10512 g        10643 t
ORIGIN      
        1 attatctgaa cataaaacac tatcagtaag ttggagtcat taccgaagcc tccataacgg
       61 aggcttcttt tttatggctg gcagatgctt taatcatcca ccttaaaaca atataaccta
      121 ttgttttaat gacaaatcag aacggaatgt catcatcaaa gtccatcggc ggctcgttag
      181 acggcgctgc cggagcggac tgctgcgggc gagactgcgc gccgccgctg aactgattgc
      241 caccctgcgg ctgctgaggc tgaccccaac cgccctgcgg ctgaccacca ccgatattgc
      301 cacctgccgg agcgccacca ccctgacgac cacccagcat ctgcatggtg ccgccaacgt
      361 tcaccacgac ttctgtggtg tagcgatcct gaccggattg atcggtccat ttacgggtac
      421 gcagctgacc ttcgatataa acctgagaac ctttacgcag atattcgctc gccacttctg
      481 ccagtttgcc gaacagcaca acgcggtgcc attcagtctg ttctttcatc tcgccggtcg
      541 ctttatcacg ccaggattcg gaagtagcca gcgtaatgtt ggcaactgcg ccaccatttg
      601 gcatgtagcg tacttccggg tcctgaccca gattaccaac gagaataacc ttgtttacgc
      661 ctctgctggc catgttcgtg tctcctgaaa aaaatcgttc tgaataagtg taaacgcgcg
      721 attgtaccat taccaatagc gcttttacta tgttgtgacc tcggttccgg gaaacaaacc
      781 tggccagaca ttgttacaca acactccgga taatgcattc caatactgta tattcattca
      841 ggtcaatttg tgtcataatt aaccgtttgt gatcgccggt agcaccatgc caccgggcaa
      901 aaaagcgttt aatccgggaa aggtgaatgg ataagatcga agttcggggc gcccgcaccc
      961 ataatctcaa aaacatcaac ctcgttatcc cccgcgacaa gctcattgtc gtgaccgggc
     1021 tttcgggttc tggcaaatcc tcgctcgctt tcgacacctt atatgccgaa gggcagcgcc
     1081 gttacgttga atccctttcc gcctacgcgc ggcagtttct gtcactgatg gaaaagccgg
     1141 acgtcgatca tattgagggg ctttctcctg ccatctcaat tgagcagaaa tcgacgtctc
     1201 ataacccgcg ttctacggtg gggacaatca ccgaaatcca cgactatttg cgtttgttgt
     1261 tcgcccgcgt cggcgaaccg cgctgtccgg accacgacgt cccgctggcg gcgcaaaccg
     1321 tcagccagat ggtggataac gtgctgtcgc agccggaagg caagcgtctg atgctgctcg
     1381 cgccaatcat taaagagcgc aaaggcgaac acaccaaaac gctggagaac ctggcaagcc
     1441 agggttacat ccgtgctcgt attgatggcg aagtctgcga tctttccgat ccgccgaaac
     1501 tggaactgca aaagaaacat accattgaag tggtggttga tcgcttcaag gtgcgtgacg
     1561 atcttaccca acgtcttgcg gagtcgtttg aaaccgcgct ggaactttcc ggtggtacag
     1621 cggtagtggc ggatatggac gacccgaaag cggaagagct gctgttctcc gccaacttcg
     1681 cctgcccaat ttgcggctac agtatgcgtg aactggagcc gcgactgttt tcgtttaaca
     1741 acccggcagg tgcctgcccg acctgtgacg gccttggcgt acagcaatat ttcgatcctg
     1801 accgcgtgat ccaaaacccc gagctgtcac tggctggcgg tgcgatccgt ggctgggatc
     1861 gccgcaactt ctattacttc cagatgctga aatcgctggc agatcactat aagttcgacg
     1921 tcgaagcgcc gtggggcagc ctgagcgcga acgtgcataa agtggtgttg tacggttctg
     1981 gcaaagaaaa cattgaattc aaatacatga acgatcgtgg cgatacctcc atccgtcgtc
     2041 atccgttcga aggcgtgctg cacaatatgg agcgccgtta taaagagaca gaatccagtg
     2101 cggtacgtga agaattagcc aagtttatca gcaatcgccc atgcgccagc tgcgaaggga
     2161 cgcgtctgcg tcgggaagcg cgccacgttt atgtcgagaa tacgccgctg cctgctatct
     2221 ccgacatgag catcggtcat gcgatggaat tcttcaacaa tctcaaactc gctggtcagc
     2281 gggcgaagat tgcggaaaaa attcttaaag agatcggcga tcgcctgaaa ttcctcgtaa
     2341 acgtcggcct gaattacctg acactttccc gctcggcaga aacactttcc ggcggtgaag
     2401 cccagcgtat ccgtctggcg agccagattg gtgcgggcct ggttggcgtt atgtacgtgc
     2461 tggacgagcc gtctatcggc ctgcaccagc gcgataacga gcgcctgttg ggtacgctta
     2521 tccatctgcg cgatctcggt aataccgtga ttgtggtgga gcatgacgaa gacgcaattc
     2581 gcgccgctga ccatgtgatc gatatcggtc ctggtgcggg tgttcacggc ggtgaagtgg
     2641 tcgcagaagg tccgctggaa gcgattatgg cggtgcctga atcgttgacc gggcagtaca
     2701 tgagcggtaa acgcaagatt gaagtgccga agaaacgcgt tccggcgaat ccagaaaaag
     2761 tgctgaagct gacaggcgca cgcggcaaca acctgaagga cgtgacgctc acgctgccag
     2821 tcggtctgtt tacctgcatc acaggggttt caggttccgg taaatcgacg ctgattaacg
     2881 acacactgtt cccgattgcc caacgccagt tgaatggtgc gaccatcgcc gaaccggcac
     2941 cgtatcgcga tattcaggga ctggagcatt tcgataaagt gatcgatatc gaccaaagcc
     3001 caattggtcg tactccacgt tctaacccgg cgacctatac cggcgtgttt acgcctgtgc
     3061 gcgaactgtt tgcgggcgta ccggaatccc gtgcgcgtgg ttatacgcca ggacgtttca
     3121 gctttaacgt ccgtggcggg cgctgcgaag cctgtcaggg cgacggtgtg atcaaagtgg
     3181 agatgcactt cctgccggat atctacgtgc cgtgcgatca gtgcaaaggt aaacgctata
     3241 accgtgaaac gctggaaatt aagtacaaag gcaaaaccat ccacgaagtg ctggatatga
     3301 ccatcgaaga ggcgcgtgag ttctttgatg cggtgccagc tctggcgcgt aagctgcaaa
     3361 cgttgatgga cgttggtctg acgtacattc gcctggggca gtccgcaacc acactttctg
     3421 gtggtgaagc ccagcgcgtg aagctggcgc gtgagctgtc aaaacgcggc accgggcaga
     3481 cgctgtatat tctcgacgag ccgaccaccg gtctgcactt cgccgatatt cagcaactcc
     3541 tcgacgtgct gcataaactg cgcgatcagg gcaataccat tgtggtgatt gagcacaatc
     3601 tcgacgtgat caaaaccgct gactggattg tcgacctggg accagaaggc ggcagtggcg
     3661 gcggcgaaat cctcgtctcc ggtacgccag aaaccgtcgc ggagtgcgaa gcttcgcata
     3721 cggcacgctt cctcaagccg atgctgtaat cgttaaggcc gctttctgag cggccttttc
     3781 ctttcagagt tgtaccagca atttacgctt ttcttccggt agtaaattca ctgcctgctg
     3841 ataagacgca tccaccagat aatagatttg cgaatccggc agcgaaccgt cgagatacac
     3901 ggtgctccag tgcgctttat tcagatggcg gctaggacgc acatcgctgt gctgctgacg
     3961 tagcagctcc gccagctccg ggctggtttt cagcgaaaca gctgggcgat tttctacttc
     4021 tttcaccatc gcaaacagta catcttccac tttgatctgc gtcgctttcc agtcgttatg
     4081 cacgctctgt tctgcgcttg gttttgccat gcaatattgt agcaactccg aaatggtcat
     4141 tttttactcc ccttgtagtg tcgcgatgat gcgacgcgat ccgccgtgga tgcgatgttc
     4201 ccccagccag atgccttgcc aagtgccggt ctgaatacgc cctttatgta ccggcaatac
     4261 aagcgatgtt cccagcattg aggatttgat atgagaaggc atatcgtctg ctccctcata
     4321 gtcatgctca taatttccgt tgtcgggaac ggtgcggagg aaaaaacact ccatgtcgtg
     4381 gcgtacggtg ggatcgcagt tctcattaag tgtcagagag gcggaggtat gttgcagcaa
     4441 cagatgcagt aagccgatgt taacgcgcgg catatcagcc agctgattca gaatttcatc
     4501 cgttaccaga tgaaacccac gagatttggc gctaagcgtg agcgtctttt gataccacat
     4561 gtgctgctcc ttgataaaac tctcttaatc agtttgcagc aagacagcga aaggataaag
     4621 gtgtgattaa aaaaacagca ttcgggagag catcactctc tcccgctctg tcagtattct
     4681 gaattgacga tcacctcttc accaaacgca cccgcttgtg gcaagggttt gtaggtagag
     4741 ttggaggcgc gcagaatgcg gataccacga gcgccgacat cgcgtgcggc ggtaatatcg
     4801 ttatcggaat cgccataaaa aattcggata tttttatcct gcagccattg cgattttgta
     4861 ttttgccctg gtttatcgcc cgcaaagatt accggattca tgttggtggc aggaatatga
     4921 aaattatccg ccagcgtttt tgaaaccgtt tctgttttcg tcgggctacg accagtcaca
     4981 aagaagatcg cgtcaccgcg gcgtacatgc atatcaatca gctggcgagc gacctctttt
     5041 ggaatgctga attcatccca gccattattc attttttccc agaacacagg atttttcaga
     5101 taatcttcgc tttctggcga gaaggttttt ttgccgcgcc agaaccccgg actggaaaaa
     5161 agtaccgtgt catcgatatc aaaccccacc gccattggcg gacgccctgc gaggctattt
     5221 tcaatttgtg cgaccgaaac ccaatgaatt ggtgcctgtt cagcaagcct ggcaacgtta
     5281 gtcccagggt taagcggtga aggagatgag gccagggcaa cagcggaact gtttagagcg
     5341 aacaataagc aaacggcact gattgcctgt gtgatcttgc gcatattttt ccctaaatag
     5401 tcagtatgtt gaaacttttt attgtgagat ttgttgcaaa aaccatctga ccataacgcc
     5461 agctgccggg gaaaggaagg attatctgcg gtttttgtga tgaaaagcag gatgattata
     5521 tttaacccag taaatacgtt cctgtctttt tttgtataaa aaatgtttat tactcgatgt
     5581 aaggatacat taattttacg tttattatga tgaatcctcg gcaaatgagc aaaatttgat
     5641 agaacattta tctgctgtac gatttaatga acttaatgat acagcattac aaaaacaatc
     5701 gaagtttata aagatgattt ctgattgacc agccccctac cctacaatgg tgattaattt
     5761 tattactcgc gatggagatg atgatatgaa tatatcatat gtgaatagca acaaaacgac
     5821 atctttacct gttgagctag atgcactaaa taataaagat atctcttatg ccaaagattt
     5881 ctttttatac atagaaacac agttaaaaat tgcaaaagat ttttgtagac ctggagaaga
     5941 agtatcaagt tctattgcaa gtaaagtttt tcacgcattt attgatttag ttaacaaaat
     6001 caggggtaag aaagacttta tgtatatttg cacgctttgc tgttttgccg aggaggttaa
     6061 aggtgattat tctcattaca ggaccttttt atttgatatt ggtaatcaat ataaggttaa
     6121 acttacacaa agcggaaaaa agagttctct ttaacattag aatttaacga tactataatt
     6181 gaatctcaga aagtcacagg caataaggca aagcatattc ttgaggatat agaaatattc
     6241 tatcgtaaca aacccgatac ttattattaa atttaataat ggttagatgt gcagaaatta
     6301 ttactccaca cgaaaaaata gggaaaggaa ttccagataa ttgggataaa cttaacaata
     6361 aaagaactga caacgtttta ttatctctca cgtagaacaa cggcatcaaa aaaatcatct
     6421 acaaagaaat agtgaaagcg acgacagttt aatttcactg caggctgggt aactccagcc
     6481 tgctttcctg cattacatca ccgcagcaaa cgcctttgcc acacgttgta catttgccgt
     6541 atttaacccg gcgacacaca tgcgaccgct ggcgatgaga tagacaccaa attcttcacg
     6601 tagtcggtca acctgagcgg cacttaaacc ggtataactg aacatgccgc gctgattaag
     6661 cagataatcg aaattgcgtt ctggcatctc tgtgcttaat accttcacca attcctgacg
     6721 cattgccaga atgcgagtac gcatctcttc tacttccgcc agccagctgg ctttcaatgc
     6781 ctcgtcattc agcactgcag ccaccacctg cgcaccaaaa ttcggcgggc tggagtagtt
     6841 gcggcgaact gttgctttca attgccccag tacgcggcca gcggcttcgg catcttcaca
     6901 cagaacagaa agtccgccga cgcgctcgcc gtaaagggag aaaattttcg agaacgaatt
     6961 gctcaccaga gcgggtaatc cagcgctggc aatggcgcga atagcgtagg catcctcttc
     7021 cataccggca ctaaatcctt gataggcaat atcgaggaat gggataagct cgcgggcttt
     7081 gagaatttca atcaccgcat cccactgatc attagtgaga tcggcacccg ttgggttgtg
     7141 gcaacatgga tgcagcaaca caatactgcg ggcaggtaat gttttcagcg tcgccaacag
     7201 gtcattaaag cgcacgctgt tagtcacttc gtcataccag gggtaagtac tcacttcgaa
     7261 tccagccccg gcgaatattg ctacgtggtt ttcccaagta ggatcgctga cccagacgcc
     7321 tgattccggg aagtagcgtt tcaggaaatc cgcgcccact ttcaatgccc ctgagccgcc
     7381 aagggtttga atggttgcta cgcgctgttg ttgcagtacc ggatggtccg caccaaacag
     7441 cagcggcgca atggcatggc gatagctgtt aagcccttcc atcggtaaat aaagcgaagc
     7501 gccatggggc tgcgcattca ggcgcgcttc cgcctccgcc acggctttca attgtggaat
     7561 aattccgtct tcgttgtagt acagaccgat acttaaattc actttgtcgc tgcgagggtc
     7621 ttctttaaaa cgctccataa gcgtaagaat cgggtcgcca gcgtaggcgt caactttttg
     7681 aaacacgcga tggttctcca ggtttacggg caggtggtta aaacacaata aaccggaaga
     7741 aggcgaagat cgagtggatg ttcaggagcg aacggcaatt agcaacagag tgagactaat
     7801 gataaacgta catccgccag aggcacgacc tttataagag catgaaagaa catcaactta
     7861 ttgaattttt aggattttat tggccggata aggcattcac gccgcatccg gcacagacaa
     7921 tcaaatatta cagaacgatt aatccacgta tttcatcgcg acccttgaag tcaggcgcgt
     7981 aataagttcg taagcgctta ctttcgtcat ttcagcgata cgttctacgg gcaaaccttc
     8041 gccccataaa atgaccggat ccccggcttt gtcctgcgcc tgtggaccta agtctacgca
     8101 gatcatatcc atcgccacgc gcccgacaat cggtacttcg cgaccgttca ccagcactgg
     8161 cgtaccggac ggcgcggcgc gcggataacc atcgccatag cccatcgcga ctacgccaag
     8221 acgggtatca cgttcgctta cccaggttcc accataacca acaggctctc cggctttatg
     8281 ctcacgcacg gcaatcaggc tggaggttag tgacatcact ggctgacagc caaaatcggc
     8341 accggtggag cgatcttcca gcggcgagac gccataaaga atgatgcccg ggcgcaccca
     8401 gtcaaaatgc gactgtggcc acagcagaat gccacccgac gcggcaatgg aacgttgacc
     8461 aggtttgcct tcgcaaaagg tattaaagat agcgagttgt ttctcggttg cgccacattt
     8521 tggttcatcc gcgcgcgcaa aatggctgac gatattcacc ggctgacgaa cgtttttgca
     8581 ctgggtcagg cgatgataaa acgcctcagc ctgttccggc cttacgccca gacggtgcat
     8641 accggtatcg agtttcatcc agacggtaac cggctcgtcc aagctagcct cttccagcgc
     8701 agccagctgt tcttcgttat gcacggcggt atgaaaatgt tgcgcagaaa tcgtcggcag
     8761 atctctggca tcaaaaaagc cttcgagtaa cagtacaggt ttggtgattc cccccgcacg
     8821 cagtcgcaga gcttcttcga gacgggctac gccaaaggcg tcagcatcgg ggagcgttcg
     8881 cgcggtctca agaagaccgt gaccataagc gttcgctttc accaccgcaa ccattttact
     8941 ggcaggtgcc agttcacgaa gacgttgcag gttgtgtcgc agagcgcggc ggttaatcac
     9001 aacagttgcc gcttgcattt gtgttccttg ataattgttt gctttaatta cctaattcat
     9061 aaaataatta ttattcgtcg tcgtactgcg gccccgcata gttgtcgaag cgcgaccatt
     9121 gaccgttaaa ggtcaggcgt accgtcccga ttgggccgtt acgttgttta ccgataataa
     9181 tttccgcgat gccttttaaa tcactgtttt cgtgatacac ctcatcacga tagataaaca
     9241 tgatcaagtc cgcatcctgc tcgatagagc cagattcacg caggtcggag ttgaccggac
     9301 gtttgtcggc acgttgttcc agagaacggt tcaactggga cagcgccacc accggcacgt
     9361 tcagttcttt cgccagtgct ttcagcgagc gggagatttc tgcaatttcc agcgtacggt
     9421 tatcggaaag cgccggtacg cgcatcagtt gcaggtagtc gatcatgata agcccgatgc
     9481 cgccgtgttc acgggcaata cggcgtgcgc gggaacgcac ttccgttggc gtcaggccgg
     9541 aagagtcatc gatatagata ttgcgttttt cgagcaaaat acccatggtg ccggaaatgc
     9601 gcgcccagtc ttcatcatcg agctgcccgg tacggatttt agtctggtca acgcgcgaca
     9661 gcgacgccag agaacgcatc atgatctgtt ctgatggcat ctccagcgag aagataagta
     9721 ctggtttatc ctgcaacatt gccgcgtttt cgacgaggtt catcgcaaat gttgttttac
     9781 ccatcgacgg acgcgcggcg acgatgatca aatccgacgg ctgcaagcca gcggtttttt
     9841 tgttgagatc gtcataaccg gtgtttaccc cggtaacgcc atcgtgtggc tgctgaaaca
     9901 actgctcaat acgcgccacg gttgcgtcga gcacatcggc gatgttcttc ggcccttcgt
     9961 ctttgtttgc acgactttcg gcaattttaa agacgcggga ttcagcaagg tccagcagat
    10021 cttcgctggt acgcccctgc ggatcaaaac cggcttcagc aatctcattc gcaaccgaga
    10081 tcatctcacg gacaacggca cgttcacgca cgatgtccgc ataagcactg atgttcgccg
    10141 cacttggcgt attttttgac agctctgcca gataagcaaa accaccgacg ctatcgagtt
    10201 gcccctggcg ttccaacgat tccgcaaggg taatcagatc gataggacta ccgctttcct
    10261 gcaaacgcgc catttcagta aagatatgac ggtgtgggcg ggtgtaaaaa tcgtctgcta
    10321 ccacacgctc ggctacatca tcccagcgtt cgttatccag cattaaaccg cccaacaccg
    10381 actgctccgc ttcgatcgag tgcggaggca ctttcagccc ggcaacttgt ggatcgcgtt
    10441 cgcggggttc agcctgctgt ttgttgaagg gtttatttcc tgccatagtg aatggagtta
    10501 ccgaaataaa gaatgggtcg aaactttacc atatgaagca gaccctgacg atacgttctg
    10561 gaggacacat ggcaacacga attgaatttc acaagcacgg tggcccggaa gtacttcaag
    10621 ccgtagagtt cactcctgcc gatccggcgg agaatgaaat ccaggtcgaa aataaagcca
    10681 ttggcatcaa ttttatcgac acgtatatcc gcagcggcct ttacccgccg ccatcgctac
    10741 ccagcggatt aggtaccgaa gcggcaggca tcgtgagtaa agtcggcagt ggtgtaaagc
    10801 atattaaggc tggcgatcat gtagtctatg cgcagtcggc gttaggcgct tacagctctg
    10861 tgcataacat taatgcggat aaagcggcga ttctgcctgc ggcaatttct tttgagcaag
    10921 ctgcggcatc cttcctgaaa ggcttaacgg tttattatct gctgcgcaaa acctatgaaa
    10981 ttaaacccga tgaacagttc ctgttccacg cagcggctgg cggcattggc ttaattgcct
    11041 gccagtgggc aaaagccctg ggcgcgaaac tcatcggcac cgtaggaacc gcgcaaaaag
    11101 cgcagagcgc gctaaaagcc ggcgcgtggc aggttattaa ctatcgtgaa gagaatctgg
    11161 tcgagcgatt gaaagagatc accggcggta agaaagtgcg cgtagtgtac gattccgtgg
    11221 gcagagacac ctgggaacgg tcgctggatt gcctgcaacg ccgcggctta atggtcagtt
    11281 ttggcaactc atcaggtgcg gttaccggtg tgaacttagg cattctcaat caaaaaggct
    11341 cgttgtatgt gacacgccct tccctgcaag gctatatcac cacgcgggag gaattaaccg
    11401 aggccagtaa tgaactgttc tctttgattg ccagcggtgt gattaaggtc gatgtcgccg
    11461 agcagcagaa atatccgctg aaggatgcgc agtgtgcgca tgagattctg gaaagccggg
    11521 cgacgcaagg ttccagcctg ctgattccat aaaagaaata gggcttccac ctgggaagcc
    11581 ctttcttttt atagttcggc tgtatgtagg gtacagcacg atgaatctgt tagaggcgca
    11641 atagtgacag atttgattat caattcctat tttgttctaa ggataaaacc ttaggttgtg
    11701 atcatccgca caatccctta gtaacgccag cggtcataac gctgatattt cggcattttt
    11761 ggtgctttaa tcgccttaat aacccacacc accgcaatcg ccagtagtaa ccacggcagc
    11821 aacttaatca tcaatgccag cataccgccg aggaacataa tggccgtcgc cacaaccagc
    11881 gcggcgataa tgcccagcaa cgaaacgccg gtgaccatca gcatgacaaa aaagccaatc
    11941 acaaaaagta gttccagcat gatgctctcc caaatatgaa atctcttgct ggcattacaa
    12001 gaatcatgcc aaaaataatc tattgattta acagcaaaac gccccgcgac ggtgcgcagg
    12061 gcgtggtgaa tttgactact ttttggtgaa aagttaacgc ttatccgcca ccagtttgag
    12121 cgcgtgttcc agcacgttaa tgtcagcacc cgctttatgg gcattttcac ttaaataacg
    12181 ccgccactgc cgcgcgccag gaataccctg gaacaaaccc agcatatgcc ggataatatg
    12241 gccgagatac gtcccctggc tgagttcatg ctcaatgtac ggatacatgg cacgcactac
    12301 tgccaccgga tcggcatcgg tatccgagga accaaagatc tcccggtcta ccgccgccag
    12361 aatacccgga ttctgatacg cctcgcgccc gaccatcacg ccatccatat gttgcaaatg
    12421 cgctttagct tcttccagcg acttgatacc accgttaatc gacattgtca gatgcgggaa
    12481 gtcacgcttc agttgataca cacgcggata atcgagcggc gggatttcac ggttttcttt
    12541 cgggcttaac cccgaaagcc aggctttacg tgcgtggatg atgaacatct cacactcgcc
    12601 tttgccggaa acggtattga tgaaatcgca gagaaattca tagctgtcct ggtcgtcgat
    12661 gccaatacgc gttttcaccg tcaccggaat cgacaccaca tcgcgcatcg ctttcacgca
    12721 gtcggcaacc agctgcgcat tacccatcag acacgcacca aacatgccgt tctgcacccg
    12781 gtcagacggg cagccgacat tcaggttgat ctcatcatat ccgcgcgctt ctgccagctt
    12841 tgcacactgc gccagcgccg ccggatcgct accgcccagt tgcaacgcta ccggatgttc
    12901 ttcttcactg tacgccagat aatcaccttt accgtgaata atcgcccctg tggtcaccat
    12961 ttcggtataa agcaacgtat tgcgggaaag cagacgcaag aaatagcggc aatgtctgtc
    13021 cgtccagtcg agcattggtg caacactaaa ccgaccactc cagtgaacgt cagttttttc
    13081 aggcattgcg ctggtttggt tgattttttg catttcagaa ttaccgtgca ttttcaaatg
    13141 tagagattat tttatcgata tatcatgggg ttatgttatt cagcttcact gttcaggagg
    13201 ctcaatagcg gggtactata ccataacaac aggaagcgcc tgtctcattg caaaagaaaa
    13261 ttgagatcct ctcaaggcat gaagctctca cgaagtgatg gaaataatct tattagccgt
    13321 taactttgtt aaggccaatg ataaacaatc caggttcgac gataaataaa aaatcacaca
    13381 ttaaactctg gtgatatatc tccctgctaa tagcattgat agagaaaaaa gaacccaata
    13441 aatattgggt ccttttatat aatgccttcc atactatcga agaacttcac atattatttc
    13501 tccgatttaa ccccgaacaa gtcataaatt aatttagaag tgtctgtaag tattttaatc
    13561 tcttcctttg aggttgggtc aaacgacttc gcgaagctaa ttaattgtgg tgcagcatct
    13621 cgcattttgc ttaaaatatc aggatcgagc gttccttcat tcaccagatg tgacatctta
    13681 tccagatagc catcaaaatt catttctccc ccatctgcca gacgcttcat ctctcccagg
    13741 tacttcttca tatcacgttg atataatttt tcaaactgag ctttcaggta gctctcatta
    13801 ttttcattaa tatacactgt ttctgataaa tctgcgtagg cactagaata tgaaagtgaa
    13861 attaacagcg aagcaaataa ttttaagctg ttgtcatcat gttcctggca ggcattaaca
    13921 aacgttaaaa aaccagaacc tattgcttga aaatgtatat tcgctaaagg ttcattatcc
    13981 ttagattcct cataaaatag ctgaaccgtg gagggaaggc cattcttagt ttctacatca
    14041 aaagtacatt gttcaatggg cattgcatct ttattatccc caggattaca tatactatta
    14101 atcgcgaaat gaaaaatagc ccggtcaaca actgatgcaa taaagtcacg atcgttcaaa
    14161 tcctcatcag tgcaatcgtc agtatactta ttgacccaca ttttgagatc atttatgtgc
    14221 tcaattaaaa acacattttt ttacgtgtat gccataaatt atgcgtgatt cgttgaataa
    14281 acaaatctat ttttgcaata attatatccc gatgacgcag cgctgcataa tcaatagatt
    14341 taccatatat attatttaat ttacctttga ttgaacctct attatccaat ttatctttgg
    14401 ttgcatcttt attttcgaat tgatttgaat tgagtgtttt attaaagtat ccagttgcac
    14461 cgggtattat cttcaacatg attcatctac cttttttgtc gtaccgtgtc ctccttataa
    14521 ttcttttgcc atctggtcac tatcaaaaat cgctcataat ttctgaacta caggtatgcg
    14581 tttaaaccca gttaaataaa tagcgagtta acttgaagca atttatttaa aaatgaatta
    14641 ttaattatta tcgtgttgat aatggtacga caaggcaaca caacacctta cctacaaaac
    14701 agaaaatcaa cattacggca acaataaggg ttctcggtgt ttttcctgga acatggtaaa
    14761 gtaaggacat tcttaacccc cactttgagg tgcccgatgg gaaagaccac aacgcaggag
    14821 ttattagcgc aggctgaaaa aatctgcgcg cagcgtaatg tgcgcctgac cccacagcgc
    14881 ctggaagtgt tgcgcctgat gagtctgcag gatggcgcta tcagcgctta tgatctgctt
    14941 gatttactgc gcgaagctga accgcaagcc aagccgccaa cggtttatcg cgcgctggat
    15001 tttctgcttg agcaaggttt tgtgcataag gtggaatcca ccaacagtta tgtgctctgt
    15061 catctgttcg accagcccac gcatacgtca gccatgttta tttgcgatcg ctgcggcgcg
    15121 gtgaaagaag agtgtgcaga aggcgtggaa gacattatgc atacgctggc ggcaaaaatg
    15181 gggtttgccc tgcggcataa tgtgattgaa gcacatgggc tctgtgcggc atgcgtggaa
    15241 gtggaagcgt gtcgtcatcc tgaacagtgc cagcatgatc actctgtgca ggtgaaaaag
    15301 aaaccgcgtt aagagggcgt acatccttgt acacgtcggg caggagggat taattaccag
    15361 cgatattcat tgcgggtttc ccaatccacg acctcttttt ctgcctgatc tttctgataa
    15421 ccataacgtt cctggatttt accgaccagt tgatcacgtt taccttcaat gatcgtcata
    15481 tcatcatcgg tcagtttgcc ccattgctct ttcactttac ctttaaactg tttccagtta
    15541 ccgccggctt catctttatt cataatcaag acctcatcgt taggttgtga atgagagtac
    15601 gttcactttt cttctgaacg tgagattaag tgtagtcagc aatttggctt aggataattt
    15661 attcagaatt tttaaccgtc acgttgcggc aaaccaggta ccgttgcgcc agtgacgccg
    15721 ccagatagcc gccagagaaa gcccgcgcaa cgccagaaag acggttaatg ccagccacaa
    15781 accatgatta cccagccacg gcagcgtaag gagcgtcagc gcaaaacctg cggcggccac
    15841 cgctatactg ttacgcattt cggcggcacg cgttgcgcct ataaacatgc cgtccagcag
    15901 ataacaccag acgccaacca acggcaaaat cacctgccag ataagatagc ggtcagccag
    15961 ctgctgaatc tgggttaacg acgtcagcaa cgcaatgatg tgttcccctg ccagcaaata
    16021 aaccaccgaa aacagtaacg ctacaatccc cgactggcgg cacgctgccc gccatacatc
    16081 cagcaactgg ctaccgtcgc gcgcaccgta tgcctgaccg gagtgcgctt caaccgcgta
    16141 ggcaaaacca tcaagcgcat aggcggtaaa ggtgagtagc gtcatcagaa ccgcgttaac
    16201 agcgataatg tcactcccca gtcgcgcacc aagtacggtg atcgcgccga aacagagttg
    16261 caacaacagc gaacgcagca tgatatcgcg gttaagcgcc agcaagcgac ggaagtttcc
    16321 tcgccaggca gttttcagca tttcgccgga aattccgcgt agtttgagga ttttacgcac
    16381 cattagcaga ccaatcagca atgttgcata ttccgcaata accgtcgcca gcgccgcgcc
    16441 ctgcacgttc atatgcagcc ccatcaccag ccagacatcc agcacaatgt tgaggatatt
    16501 gccgaccact aacaaaatta ctggcgcacg ggcatattgc acgccgagta accaaccgag
    16561 taataccaga ttcgccagcg acgccggagc gcttaaccag cggatttcaa gaaagcgccg
    16621 cgcctgttcc aggaccgctt cactaccgcc aacaatatgc agcgccagat cgataatcgg
    16681 cgtacgcaac agcgcaatta acgccccggc ccccaacgcc aacagcaacg gttgcaccag
    16741 cgcacgggct aatgcctgag gatttttggc accataagcc tgcgcagtca gcccggtggt
    16801 gctcatgcgt aaaaacagca acagcataaa gagaaagctg gtcgccgttg caccaaccgc
    16861 cacgccgccc aaataaaccg gactatcaag atgaccaatt accgccgtat cgaccagtcc
    16921 cagcaacgga acggtgatat tggagaaaat catgggtaag gcgagatgcc agagtgcttt
    16981 atcagatgaa gtgaggaatg ccatgcagac aagcctgatg aagagagatg aaaaacaaac
    17041 cgcgacgcca agcggcatcg cggtctcaga gatatgttac agccagtcgc cgttgcgaat
    17101 aaccccaacc gccagccctt caatggtgaa gctctgctga cgaaggtcaa cgacaattgg
    17161 tttaaactcg ctattttctg gcaacagttc gactttattg ccctgttttt tcaggcgctt
    17221 aacggtaact tcgtcatcaa tacgtgcgac aacgacctga ccgttacgta catcctgagt
    17281 tttatgcact gccagcaagt cgccatccat aatgccgata tctttcatcg acatcccgct
    17341 gacgcgcagc aggaaatcag cattcggctt gaacaaggaa ggatcgacct gataatgacc
    17401 ttcaatatgc tgttgcgcca gaagcggttc accggcagcc acacgaccta ccagcggcaa
    17461 cccttcttcc tcttcctgca acagacgaat cccgcgtgat gcgccggaaa caatttcaat
    17521 aacgcctttg cgtgccagcg ccttcagatg ttcttcagcc gcgtttgggg aacggaaccc
    17581 caaacgctgc gcgatttccg cacgcgtcgg cggcatacct gtctggctga tgtgatcacg
    17641 gatgagatca aacacctctt gttgcctggc cgttaacgct ttcattccgc cccctgggtg
    17701 tatatacagt tatactgtga gtatatacag caaaaggcga ttttggaacc ataaactgca
    17761 caataaacca gagatttatc gaaatctgga agggttatcc aaaatgcgac cataacagga
    17821 tgcaccaggt aatcacggcg acgataatgg caatcagcac cgcggcggat cccatatctt
    17881 ttgcgcgtcc ggaaagctca tggtattcag agccaattcg gtcaaccact gcttcgatgg
    17941 cgctattgag gatttccaca atcatcacca gcatcacgga gctgataagc agcacgcggg
    18001 taattgcgtc cacatccagc cagcaggcga tgaccaccgc caacaatacc gctacgcctt
    18061 cctgacggaa tgccgcttcg ttgatccatg cagcgcgtaa acctttccag gaatagccag
    18121 cagctttgat aattcgggtg aatccagttg tattattggc cattgaaaga acctttttac
    18181 attatgagcg tcaatatcag tgtaccgtta aatccgatga ttcgggtttg cgtaacgccg
    18241 cgcgaaacat gagcggatac cacagaattt cccatgactt tctgctatcc ttgccgcgca
    18301 tttgcattat taaccagagg ctttacatcg tttatgtccg gctggccacg aatttactac
    18361 aaattactga atttaccatt aagcatcctg gtaaaaagca agtctattcc ggcagatcct
    18421 gccccggaac tggggctgga tacctctcgt ccaattatgt acgttttacc gtacaactcg
    18481 aaagcagatt tgctgacgtt gcgcgcccag tgtctggcac atgatttgcc tgacccgtta
    18541 gagccgctgg aaatcgacgg cacgctactg ccgcgctatg tgttcattca cggcgggccg
    18601 cgtgtattca cctattacac gccgaaagaa gagtctatta agcttttcca cgactatctc
    18661 gatttgcacc gtagcaaccc aaatctggat gtgcagatgg tgccagtatc ggtgatgttt
    18721 ggtcgcgcgc cggggcgtga aaaaggcgaa gtgaacccgc cgctgcgtat gcttaacggc
    18781 gtacagaaat ttttcgctgt actgtggctc ggtcgcgaca gttttgtgcg tttctcgccg
    18841 tcagtgtcgc tgcgccgtat ggcggatgaa cacggcacgg ataaaactat cgctcagaaa
    18901 ctggcgcgcg tggcgcgtat gcactttgcc cgtcaacgtc tggctgccgt aggcccacgt
    18961 ctgcctgctc gtcaggatct gtttaataag ctgctcgcct cccgcgccat tgccaaagcg
    19021 gtagaagatg aagcgcgcag caaaaaaatc tcccatgaaa aagcgcagca gaacgcgatt
    19081 gcgctgatgg aagagattgc ggcgaatttc tcttacgaga tgatccgcct gactgaccgc
    19141 attctgggct tcacctggaa ccgattttat cagggcatca acgtccataa cgccgagcgc
    19201 gttcgccagc tggcccacga cggtcatgag ctggtgtatg tgccttgcca ccgcagtcac
    19261 atggactacc tgctactttc ttacgtgctg taccaccagg ggctggtgcc gccgcatatc
    19321 gccgccggga tcaacctgaa cttctggccg gccgggccga ttttccgccg tctgggggcg
    19381 ttctttattc gccgtacttt taaaggcaat aaactttatt ccaccgtttt ccgtgagtat
    19441 ctcggcgaac tgttcagccg tggttattcc gtcgagtact tcgtggaagg cggtcgttcc
    19501 cgcacggggc gtttgctgga tccgaaaacc ggtacgctgt cgatgaccat tcaggcgatg
    19561 ctgcgtggcg gcacgcgtcc gattacgctg attccgatct atatcggtta tgagcacgtc
    19621 atggaagtgg gtacttacgc caaagaactg cgcggcgcga cgaaagagaa agagagcctg
    19681 ccgcagatgc tgcgcggttt aagcaagctg cgtaatctcg gtcagggtta cgtcaacttc
    19741 ggtgaaccaa tgccgttgat gacctacctt aaccagcacg taccagactg gcgtgaatct
    19801 atcgatccca tcgaagcggt gcgtccggcc tggttaacgc cgacggtcaa taatattgct
    19861 gccgatctga tggtacgcat taacaacgca ggcgcggcaa acgccatgaa cctgtgctgt
    19921 actgcgctac tggcatcacg tcagcgctca ctcacccgcg agcagttaac cgagcaactc
    19981 aactgctacc tggatctgat gcgcaacgta ccttactcca cggactctac cgttccttca
    20041 gccagcgcca gcgagcttat cgatcacgcg ctgcaaatga acaagtttga agtcgagaaa
    20101 gacactatcg gcgacatcat cattctgcca cgcgagcaag cggtgctgat gacctactat
    20161 cgcaacaaca ttgcgcatat gttggtgctg ccttcgctga tggcggcaat tgtcacccag
    20221 catcgccaca tctcccgcga cgtattgatg gagcacgtca atgtgcttta cccaatgctg
    20281 aaagcggagc tgttcctgcg ctgggatcgc gacgagttgc cggacgttat tgatgcgctg
    20341 gcaaatgaga tgcaacgtca ggggctgatt accctgcaag atgatgagtt gcatatcaac
    20401 ccggcgcatt ctcgcaccct acagctgctg gccgcaggcg cgcgcgaaac gctgcaacgt
    20461 tatgccatca ccttctggtt gttgagtgcc aacccgtcga tcaaccgcgg tacgctggag
    20521 aaagagagcc gcaccgttgc gcaacgtctc tccgtgctgc acggcatcaa cgcgccggag
    20581 ttcttcgaca aggcggtgtt cagttctctg gtgctgacgc tgcgtgatga agggtatatc
    20641 agcgatagcg gcgatgccga accagcagaa acgatgaagg tttatcagtt gctggcggag
    20701 ttgattacat cagacgtgcg tttgacgatt gagagtgcga cgcagggcga agggtaatca
    20761 gagagaattg ccggatgcgg cgaaaacgcc ttatccggcc taccatgacc tgcaaattca
    20821 ataaattgcg attcaccggg taggcctgag aagcgcaacg catcaggcaa cccagaagaa
    20881 agccggatga tcatccggct tttttacatc atcagaaatg ccagtaactc attgccagtc
    20941 ctaaaaacag taccagacca acatagttat tattcataaa tgctttaaag caggcttcac
    21001 gctcgcggtt ggcaatcagt ttttgttgat aaacaaacag cgcgccagcc accacaattg
    21061 accagtaata tccccagcct aagccattta actcaccgat gatcgccatc agtgccagta
    21121 cgccaatctg caaaatacca ataatcaatt tatcgtattg gccgaacagg attgccgtgg
    21181 atttaatgcc aatcttcaca tcatcatcgc ggtcaaccat cgcatactgc gtgtcgtaag
    21241 ccaccgccca gagaatattg gcgaggaaca ttaaccagca actcaatggc accgactcac
    21301 tcacagcggc aaaagccatt ggaatcgacc agccaaacgc cgcgcccagc accacttgcg
    21361 ggagatgggt ataccgcttc ataaacgggt acacccacgc caatgccaga gcggcaatcg
    21421 acaacagaat ggtcatcgta ttcagcgtca gaaccagtaa aaacgaaatc agtaccagca
    21481 ctacaaacag cgcgcgcgcc tctttctctg ttaccgcgcc gctgggaagt ggtcggttcg
    21541 ccgtgcgctt aacatgacca tcaaacttgc gatcagcata atcattcacc acgcaaccag
    21601 cagcgcgcat cagccagaca cccgcgacaa acaccgccat gatccagagc tgaggaacgc
    21661 ccggtgtcgc cacccacaac gcccataatg ttggccagag cagcagtaac gcgccaattg
    21721 gcttatccgt acgcattaag cgatgaaacg ccagcagctt attctgcgtc agactccact
    21781 ccatattttt ttcctcttag tacaacggtg acgccggtaa aaacagttct gttagcaaca
    21841 gcggtttacc gcttaatcgc aggcgggaac gtcgccccca cagtccggca tcacggccta
    21901 tctcgataaa atcccgggtt agtgtcgatg atgtgaacag atagcgtcct aacggcgttt
    21961 tacccaattt ttgtaacgct agttccggcc cgcttaatgt tgatacagga acgacggtac
    22021 gaccggcaag ccacggttca ccatcggcac ataacaaaat ttcacgtaac cagtaacgag
    22081 actctttcgg cagcagcggc agttcttcgg ggatttcatt ctgctcgaca aacccctcgc
    22141 ggatcatcgt cacactcacc gtttttccct gctgttcaaa acgttttgtc atggaatcct
    22201 ccagcaacag ccagtcgagc agttgcggat ccagggcagg gatctcttta aaatagcgca
    22261 gcgcacgcag ttgcgttaac gcggggtgtg acatgccgaa ctctccgtta cataacgtaa
    22321 aggcattgta tcgcagaaaa gtgtttacgg gagatgtaaa gcaaattaag tgatctaatg
    22381 cgcaacaaaa acgcaacaat cgcatgaagt ggtaaaacgc tgttgaacct tacgctttgc
    22441 cgatgcccgt gctggcgagg gcaaccatag gggcatcacc gcccatgatg ctaggaacgg
    22501 tgatgttttc ctttacttaa atacctttat tctttatacg agtagctcgc caactcgcag
    22561 taaaaattgg gaaaagcgca tatttaccat gagatttgga gctgaaaagt gtttcctgag
    22621 taagattaat aggcaacatc ttatgtctgg atttatagat acacgtggaa ttccaattgg
    22681 tatcttttgt aggattcaac atatcatgta agcgctggga gcttaccacc ataacaggcc
    22741 catcatcgac agaggagaac aggataaata tattatcgtt tcgtgttaat ttattagtgt
    22801 taaaggttct taacttttct tcagtttcta taaatatagg tgaaacatca tcactacgaa
    22861 tccactcttt ataaagatac caggcatttg ctttgatcat atcatccgtt gatcgtaagg
    22921 cctgatcgat aagttggata aaaaaattgt tatttttatc aaccagccct ttattcatat
    22981 taaatctgtt aataaaatgg atcatctgtt gggtatcgaa attcacctta ctgaatcgat
    23041 gttgacgcat aaaattatcg aacttgtcat cttccccaga atgtatataa ctacgtataa
    23101 ttggatcttt tatataagga caatggctaa atgttttctt catattctta ataaagtttt
    23161 tattaacaat attgagaatt tttaattgca tcaccagttt tactgccaga acggatttca
    23221 atatcattgc attatctggc aatgtatcaa tcatttttat aatgttattg atataaccat
    23281 cgagatcttc attcccaaat atatctgaat aatcaatagt taattccaat tgttctgtaa
    23341 tgcaatctta aaaattccct gtcgacattc gcacatgatc aaacataaca gctgtcaaat
    23401 tcgatttaat aaatgatatt tctttggcag acagaaactc tgctctggtg aagtgcgaag
    23461 cgtaaagata acaattatcg aagtttgaat ttctaatctg acattcgcga aaattactat
    23521 gaccaagttg actctgcgag aagtcaactg tatcaagaat attttggcta aaatcaacac
    23581 attcgccact cgttttgaga aaaatcacat tgtgtaaacg tcgacattta aatatgacat
    23641 tatcaactgt tgccttagta aaattgcaat tctcaatttc tttacattcg aaagttacat
    23701 gttgtagatt tgatgataaa aaagacacct tagaaataac gtcaccttta aaatgacaat
    23761 ttgttaaatc aaaatcagac aggtcgatat acccctgcgc atctttatcc atcagaaaat
    23821 tacaagcgca ttctattttt tcatttctgt agaattttac acttatagta tcattactaa
    23881 ttgagacttc acgtaacata tttattgcat gatttttttc gttccgttgt tttatattta
    23941 aaaatatatc actgatataa ataaagtaat ctctgtttaa acctgtcacc ccattgaaat
    24001 tacgtagtaa atcaagatct actatattat ttccttgcac cacatcatgt ttcatcaaag
    24061 agttattgcc gccagaattt gtgatatgac ctatcataaa acctctgtaa tacagaaata
    24121 aaataacctg gaaaagtagc ctgagcgtcc agacactacc ggcgaaagga aaagaatctc
    24181 tgataatgca ttgaaataat ggacattctt agtgaagaag aagtgaagta ctataaaaaa
    24241 tcgctcatat cccaggaagg aagcataaaa aaaggtgcgc caggagacgc accagttgtt
    24301 gcaaaatcag cactgtgggg cgtaattacc ccttgccttt tacactgctg ataaaggtgg
    24361 aacgggcaga tgtcgatccc aggcgttcag cttcatcaag cagttttaac gccttatcaa
    24421 catcaccttt cgcgacagcg tttttgatcg cggtattaaa ataactttcc gtgtcgttga
    24481 gcatcggctc gcttttcttc accggcaccg gagcaggtgc agccacagct ggtgccgccg
    24541 tgttgcctac cgtaaccgga gctggagcgg aagaaccaaa tagtggtcct accaacacgc
    24601 tggagctgga gttcgttttg actttcagtt tcagtaagcc atcgctggta tgacgagcaa
    24661 ccggatcggg aatatccggg atcgagttac cgacgccttt ggcataggct ttagccgggt
    24721 cgagcagttg ggtcgtctgc tggagatctt tttccgtggt aaagaccaga acataaagtt
    24781 tttgctgccc caacgccggt gtcaggcgca taacgccttc cagccgctct gcactcatca
    24841 cgcctagttc ctggtaggtg aaataactgc tggggaagaa ggctgatggg gtcatgttct
    24901 gatcaagaat cagcacgttc ggcgcaaaaa cgctggtttg tttgttcact tcgctggtca
    24961 gcgtcagggg cagttcgcca atgtttgccg ggacgctgta cgcagcaacc ggaccactga
    25021 tgccgggaac gttcagttgt tggccgccgg tcgccagttg ggtggtctgg gttctagatt
    25081 gatcgaccgg tgtccaggtg agttgttgca gcgcggcaga tggaatggct ggcgcgtcgc
    25141 tggtgttttg cggtacgtag ttaacatcgg caaggctaat tccaggcgcg cttgccagta
    25201 accctgctga taaacagagg gcgatgagac ttttattcat tttcattgtt ttcacctcaa
    25261 aatctggagc tcagcggtag ccaggcaata gcgcgctaaa cccgataatc agaggggctt
    25321 tcgccccttt cagataatga caacctgttt ttatgccgga tgcggcgtaa acgccttatc
    25381 cggcccaggt tttgctatta ccaccagatt tccatctggg caccgaaggt ccactcgtcg
    25441 ctgtcgccac gaccgaagct gccgccgttg aaatcagcag gaacggcttt gccgtagttc
    25501 gggttaacct tgctatcgcc gttgtagtcg taaccccatt tctcatccca cttggcgtag
    25561 gttgcgaaga cacgaatagc cgggcgtgac cagatgctgt cgccagcctg ccattgttgt
    25621 gcgagggtaa ttttgtactg attgttcttg tcgccggtgc gctgggattc gacgttgtcg
    25681 tagccgattt ccatcacggt gctcatgatt ggcgtccact tgtacatcgg gcgaatacca
    25741 acggtccacc acttggtgcc gttgtcgtta tcccagttga tatcctggta catacccacg
    25801 tacatcatgt cccagttgtc gcccatggag atcgcaccgt ggtcgaggat acgcagcatg
    25861 tgaccgttgt tgttgatatt gtaggcaaat ttttcgttat caaacgcgac gccagaaccc
    25921 tgcgacagac ctttaccctg cgaggtcatc gagtcagtag cgtactgaac aacaaacttg
    25981 ttaaagccct tcaggacact ctgagtatgt tcagcagtga ataaccagcc gtctttcgat
    26041 gcgccatcaa ccagacgata gttatcacgc aggttggcac gaccgtagtc gacacccagt
    26101 tctaatgtgc cgcccgggtt gatttccatc tgcgctaaac gcacatcgaa aacgtcgttc
    26161 gcggtttcgt tggtatagtc ataaatattg ttgctggcaa aagaggaaga accaccagct
    26221 tcagaggagc gggttgctgc cagagagagt ttaccgaagc caacatcgat gttttccaga
    26281 ccggcaccag gaccagaaat atcccagtag tagaagtcga tcatatgaac gtcatgacgt
    26341 tggtagaagc gcttacctgc ccagatggtg gaacctggca gccattcgat caggtttttt
    26401 ccctgcacgt ttgcttcacg gaaggccgga tcggtagctt cccagtcatt ctgttgcgcg
    26461 acggaatagg ccacgttagt gtcgaaatag aagctcttat cgccctcttt ccacacttcc
    26521 tgacccaatt ttaattcagc ataagtttca cattcgttgc caagacggta tttactttga
    26581 gcaccggtag tctggaaaca ctgttgttca ccgccgctac ctgtccagcc aataccggaa
    26641 cgtgcatagc cgtggaaatc aacagccatt gcctgagcag acattacgcc cgctgcgacg
    26701 gcaaccgcca gaggaagttt gcgcagagta atcatcattc tatctcctga gtcattgctt
    26761 ttcttttttc acatcacctg tgacaggctt tgtgtgtttt gtggggtgct taaacgcccg
    26821 gctccttatg cagtcgacga catgcagtgc catcctcacg gaacagatgg caacgctctg
    26881 gcggcaggcc gatagcgaat gtggcacctt cttctaccaa caccacgtcg ttctggcggt
    26941 acaccaggtt ttgacgaatg gaagggatct ggatatagat ttgggtttcg ttgccgagtt
    27001 gctcgacgac ctgaacttca ccctcaagga tgacgtcagc gatatcactc ggcagtagat
    27061 gttccgggcg aatacccagc gacatattgg ctccaacctg gacatcacgg ctttcaacag
    27121 gcagccagac ttgttgacga tttggcatcg gcagctccac ctgcacttga tcgattgcgg
    27181 tggcggtcac ttttaccggc aggaagttca tctttggcga accgataaat ccggcgacaa
    27241 aacggtctgc cggatagtgg tacagttcca gcggtttccc aacctgtgcc acgcgaccgg
    27301 cgtccagcac cacgattttg tcggccagcg tcatcgcttc gacctgatcg tgggtgacgt
    27361 aaatcattgt gcggcccagg cgtttatgca gacgggagat ttcgatacgc atttgcacac
    27421 gcagtgcagc atcgaggttg gagagcggtt catcgagcaa aaatacgctt ggctcggcca
    27481 ccagcgtacg gccaatcgcc acgcgctgac gctgaccacc ggagagcgct ttcggtttgc
    27541 gatccagcaa atgcgccagt tgcagcactt ccgccacctg gttgacgcgt tggttaatca
    27601 cctctttttt tgcgccagcc agtttcaggc caaatgacat gttttctgct actgacaggt
    27661 ggggatagag cgcgtaagac tgaaacacca taccaacgcc gcgttctgct ggcggagtgt
    27721 cattcatccg tttctcaccg atgaacaggt cgccgctggt gatcgtctca agcccggcaa
    27781 tcatgcgcag taaagtcgat ttaccgcagc cagacggtcc gacaaacacc acgaattcac
    27841 cttcatggat atcgagattg atatctttcg ataccacgac ctcgccccag gctttcgtta
    27901 cattttgcag ctgtacgctc gccatgccct tctccctttg taacaacctg tcatcgacag
    27961 caacattcat gatgggctga ctatgcgtca tcaggagatg gcttaaatcc tccaccccct
    28021 ggctttttta tgggggagga ggcgggagga tgagaacgcg gcttctgtga actaaaccga
    28081 ggtcatgtaa ggaatttcgt gatgttgctt gcaaaaatcg tggcgatttt atgtgcgcat
    28141 ccccacatta ccgccaattc tgtaacagag atcacacaaa gcaacggtgg ggcgtagggg
    28201 caaggaggat ggaaagaggt tgccgtataa agaaactaga gtccgtttag gtgttttcac
    28261 gagcacttca ccaacaagga ccatagatta tgaaaataaa aacaggtgca cgcatcctcg
    28321 cattatccgc attaacgacg atgatgtttt ccgcctcggc tctcgccaaa atcgaagaag
    28381 gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta
    28441 agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag
    28501 agaaattccc tcaggttgcg gcaactggcg atggccctga cattatcttc tgggcgcacg
    28561 accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt
    28621 tccaggacaa gctgtatccg tttacatggg atgccgtacg ttacaacggc aagctgattg
    28681 cttacccaat cgctgttgaa gcgttatcgc tgatttataa caaagacctg ctgccgaacc
    28741 cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga
    28801 gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg
    28861 ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata
    28921 acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga
    28981 atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga
    29041 ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa
    29101 cggtactgcc gaccttcaag ggtcaaccat ctaaaccgtt cgttggcgtg ctgagcaccg
    29161 gtattaacgc cgccagtccg aacaaagagc tggcgaaaga gttcctcgaa aactatctgc
    29221 tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga
    29281 agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg gaaaacgccc
    29341 agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtacgta
    29401 ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc
    29461 agactcgtat caccaagtaa tgctgtgaaa tgccggatgc ggcgtgaacg ccttgtccgg
    29521 cctacaaaac tgaaacgcat gtaggcctga taagacgcat cagtgtcgca tcaggcagtt
    29581 gttgtcggat aaggcgtgaa agccttatcc gtcctggaat gaggaagaac cccatggatg
    29641 tcattaaaaa gaaacattgg tggcaaagcg acgcgctgaa atggtcagtg ctaggtctgc
    29701 tcggcctgct ggtgggttac cttgttgttt taatgtacgc acaaggggaa tacctgttcg
    29761 ccattaccac gctgatattg agttcagcgg ggctgtatat tttcgccaat cgtaaagcct
    29821 acgcctggcg ctatgtttac ccgggaatgg ctggaatggg attattcgtc ctcttccctc
    29881 tggtctgcac catcgccatt gccttcacca actacagcag cactaaccag ctgacttttg
    29941 aacgtgcgca ggaagtgttg ttagatcgct cctggcaagc aggcaaaacc tataactttg
    30001 gtctttaccc ggcgggcgat gagtggcaac tggcgctcag cgacggcgaa accggcaaaa
    30061 attacctctc cgacgcgttt aaatttggcg gcgagcaaaa actgcaactg aaagaaacga
    30121 ccgcccagcc cgaaggcgaa cgcgcgaatc tgcgcgtgat tacccagaat cgtcaggcgc
    30181 tgagtgacat taccgccatt ctgccggatg gcaacaaagt gatgatgagc tccctgcgcc
    30241 agttttctgg cacgcagccg ctctacacac tcgacggtga cggcacgttg acgaataatc
    30301 agagtggcgt gaaatatcgt ccgaataacc aaattggctt ttaccagtcc attaccgccg
    30361 acggcaactg gggggatgaa aagctaagcc ccggttacac cgtgaccacc ggctggaaaa
    30421 actttacccg cgtctttacc gacgaaggca ttcagaaacc gttcctcgcc attttcgtct
    30481 ggaccgtggt gttctcgctg atcactgtct ttttaacggt ggcggtcggc atggttctgg
    30541 catgtctggt gcagtgggaa gcgttgcgcg gcaaagcggt ctatcgcgtc ctgctgattc
    30601 tgccctacgc ggtgccatcg ttcatttcaa tcttgatttt caaaggattg tttaaccaga
    30661 gcttcggtga aatcaacatg atgttgagcg cgctgtttgg cgtgaagccc gcctggttca
    30721 gcgatccgac caccgctcgc acgatgctga ttatcgtcaa tacctggctg ggttatccgt
    30781 acatgatgat cctctgcatg ggcttgctga aagcgattcc ggacgatttg tatgaagcct
    30841 cagcaatgga tggcgcaggt ccgttccaga acttctttaa gattacgctg ccgctgctga
    30901 ttaaaccgct gacgccgctg atgatcgcca gcttcgcctt taactttaac aacttcgtgc
    30961 tgattcagct gttaaccaac ggcggcccgg atcgtcttgg cacgaccacg ccagccggtt
    31021 ataccgacct gcttgttaac tacacctacc gtatcgcttt tgaaggcggc gggggtcagg
    31081 acttcggtct ggcggcagca attgccacgc tgatcttcct gctggtgggt gcgctggcga
    31141 tagtgaacct gaaagccacg cgaatgaagt ttgattaagg gagataacaa aaatggcaat
    31201 ggtccaaccg aaatcgcaaa aagcacgttt gtttattact cacctgctac tgctactttt
    31261 tatcgcggcg attatgttcc cgctgctgat ggtcgtcgct atctcgctgc gtcagggaaa
    31321 cttcgcgacc ggtagcctga tcccggagca aatctcctgg gatcactgga aactggcgtt
    31381 aggttttagc gttgaacagg ctgatggtcg cattacgcca ccgccattcc cggtactgct
    31441 gtggctgtgg aactcggtaa aggtcgctgg gatttccgcg attggcattg tggcgctctc
    31501 caccacctgc gcctacgctt tcgcccgtat gcgctttcca ggcaaagcga cgctgctgaa
    31561 aggaatgctg attttccaga tgttcccggc agtactttca ctggtcgcgt tgtatgcgtt
    31621 gtttgatcgt ctgggtgagt acattccatt cattggcctg aatactcacg gcggcgtaat
    31681 tttcgcgtat ctgggtggga ttgcgctgca tgtctggacc atcaaaggct atttcgaaac
    31741 catcgacagt tcgctggaag aagcggctgc gctggatggt gcgacaccgt ggcaggcctt
    31801 ccgccttgtc ctgttgccgc tgtcagtacc gattctggcg gtggtattca tcctgtcgtt
    31861 tatcgctgcc attactgaag ttccggtcgc gtcgctgtta ctgcgtgacg taaacagcta
    31921 caccctggcc gtggggatgc agcaatacct caacccgcaa aactacctgt ggggtgactt
    31981 tgccgccgct gccgtgatgt ctgcattacc gatcaccatc gtcttcttgc tggctcaacg
    32041 ctggctggtc aacggcctga cggcaggtgg tgtgaaaggt taaagatgtt gttctgccaa
    32101 tgttatgccg ctgcaccctc aacttacgtt atcccaactt gtgactatta ttcggcgctc
    32161 cacggagcgc ctttttttct ttcgtctgca atctgaatcg ttcgccggtt aatattccca
    32221 tcatagaact tattattttt acgttatttg ttttcccact tacgataatt ctctttcgtg
    32281 ctctgagtca cggcaatagt attgttttca tcaatttttg ataattatca caattaagat
    32341 cacagaaaag acattacgta aacgcattgt aaaaaatgat aattgcctta actgcctgac
    32401 aattccaaca tcaatgcact gataaaagat cagaatggtc taaggcaggt ctgaatgaat
    32461 acccagtata attccagtta tatattttcg attaccttag tcgctacatt aggtggttta
    32521 ttatttggct acgacaccgc cgttatttcc ggtactgttg agtcactcaa taccgtcttt
    32581 gttgctccac aaaacttaag tgaatccgct gccaactccc tgttaggatt ttgcgtagcc
    32641 agcgctctga ttggttgcat catcggcggt gccctcggtg gttattgcag taaccgcttc
    32701 ggccgtcgtg attcacttaa gatttctgct gtcctgtttt ttatttctgg tgtaggttct
    32761 gcctggccag aacttggttt tacctctata aacccggaca acacagtacc tatttatctg
    32821 gcaggttatg tcccggaatt tgttatttat cgtattattg gcggtattgg cgttggttta
    32881 gcctcaatgc tttcaccaat gtatattgcg gaactggctc cagctcatat tcgcggaaaa
    32941 ctggtttcat ttaaccagtt tgcgattatt ttcgggcaac ttttagttta ctgcgtaaac
    33001 tattttattg cccgttccgg tgatgccagc tggctgaata ctgacggcag gcgttatatg
    33061 tttgcctcgg aatgtatccc tgcactgctg ttcttaatgc tgctgtatac cgtgccagaa
    33121 agtcctcgct ggctgatgtc acgcggcaag caagaacaga cggaaagtat cctgcgcaaa
    33181 attatgggca acacgcttgc aactcaggca gtacaggaaa ttaaacactc cctggatcat
    33241 ggccgcaaaa ccggtggtcg tctgctgatg tttggcgtgg gcgtgattgt aatcggcgtt
    33301 atgctctcca tcttccagca atttgtcggc atcaatgtgg tgctgtacta cgcgccggaa
    33361 gtgttcaaaa cgctgggggc cagcacggat atcgcgctgt tgcagaccat tattgtcgga
    33421 gttatcaacc tcacctttac cgtactggca attatgactg tggataaatt tggtcgtaag
    33481 ccactgcaaa ttatcggcgc actcggaatg gcaatcggta tgtttagcct cggtaccgcg
    33541 ttttacactc aggcatcggg tattgtggcg ctactgtcga tgctgttcta tgttgccgcc
    33601 tttgccatgt cctggggtcc ggtatgctgg gtactgctgt cggaaatctt cccgaatgct
    33661 attcgtggta aagcgctggc aatcgcggtg gcggcccagt ggctggcgaa ctacttcgtc
    33721 tcctggacct tcccgatgat ggacaaaaac tcctggctgg tggcccattt ccacaacggt
    33781 ttctcctact ggatttacgg ttgtatgggc gttctggcag cactgtttat gtggaaattt
    33841 gtcccggaaa ccaaaggtaa aacccttgag gagctggaag cgctctggga accggaaacg
    33901 aagaaaacac aacaaactgc tacgctgtaa tcttcctgtc cagcacgccg cgccatttcg
    33961 gcgtgctgtc tttttactcc cgcttcagcc gtttcgaatt acacagccac agggtgatca
    34021 ccagtaacag gatcgcagcc gagtagatca acacatccag tggcgattta tgatcgacga
    34081 tgatcaagcg cacaatggcg gtgatcccaa tatagacaaa gtaacgtagc gggaagtgaa
    34141 aaccggactg aaagtacttc acaatcagcg cgataaattc gaaatagaga aagtaaacca
    34201 ccagtccttc taccagctca tatttgctgg tttgttctgg cgcgaacagc acatcagcca
    34261 gatgcaccgt ttctttgccg aggaagacaa ccaaaatcag gccgaggcac agcaggccaa
    34321 gattgagtac ggtctgcaaa atggtggaga taaactccac gcgcggacga gagagtgacg
    34381 tcatagcagt acctccttca cagtggcgag cttcctgtat aacgcaaaaa tgtgacggag
    34441 atctatattt tttgtgtatg ttttgttcaa cctggatttg gagcgcgcct ccaccaaagc
    34501 cggatatacc tacccgtagg ccggataagc cgcgcctggc ggcgcatccg gcaagacaaa
    34561 ccgcaccaaa gccggtaatt caaaaatcac ccattcaggt gattcccagc caccagcaat
    34621 gcgcgaaaaa tcgacaatgt tcctctggaa caaatgaaac gtaacctgat aaaggtggta
    34681 aaaatgaagc cttattttgc tgctttgatg ttatcagtct ctgttcttcc tgcttatgct
    34741 ggtccgctcg gtactgctga taaagccgac ctaccgcaaa gcaatgtctc cagcccaatg
    34801 atggcccagt cgctcaggca accagacctg caaccgatat cgacggacag gaagacagaa
    34861 tgttttcgcc tttacacacc agatcgtaaa ccgggggtga actgcgttcc tgatggctca
    34921 acaggtcatt aagaatgagg gtgccagtga gtgcgaaaac tggcaccttt cccatgactt
    34981 agcggaaatt tacgctacgg tcgctggtca tgtcatacaa ctggaactta cgcccaagtt
    35041 gctgaccacc gtcacgcgtc agcggcgtcc agccaatcgc cgcacggctg cgtgtcgggc
    35101 cagacgagaa gagatccaac ggtaccgaga catacacgcc tttggtgaag tccccttcgc
    35161 cgtactcctc tttcgaaacg ttggtgatcg tggcatagcc acccaccacc acgccgctgt
    35221 caaagcgttt agcgatctcc agcgtgccgc ctttatcccc tgccagatac tgcccgacgc
    35281 tggctttcac caacacgtcc tgggcgaaag atggcgtcca gtaggcggtc agatgtccgg
    35341 ttttcacgct gtagtcggtg aatttcatca tatcttttgc actacgccag tcgcgctgtt
    35401 taacgtagtt ggcatccaga ccaaacgccc agttactgtc cagcggacga tacaacactt
    35461 cggcccccgc accgccaaac atggtttcga gataacctcc atagacctga ccgtagaagc
    35521 cgttgcccag atgctggaag tagttggctt gcaggttatt cacatagaca tcgttctgca
    35581 catactcgcg cacatgggta cgaacgcgcg gtaagtgcga gtcctgcggt ggattggtgt
    35641 agttaaactt gtcgtagttg ttggcaagat ttgcaaacag gctgccggtg gtcagtagat
    35701 ggtccgtcag ccacaaatct gccgttccca tcacgcccag ctgatacatg taaaagtttt
    35761 ccgggccacc gaccgactgg ttcagcaccg gatcgatatg gaaatcaaag cgtgatttgt
    35821 cgatatacca gccctgctcg gtggacttcg gaaccactgg ctcgacgcgt ttgtgcgcca
    35881 gcgtcgtttc gtggcccaac ggctctccgg cgagatgatt tttcaggctg gcgacatcgg
    35941 tttccgtcgt cacttgcggc atgttaaggc gattttccgt aatgcggatc gttttgatcc
    36001 catccggcag atcgttcatc acgatccgat tagcacggat gatcccttcg cgcgaatcac
    36061 gatatttcac ctgctcgccg gtaacataca gcgtatcgcc tttcgcctgg atctgcggat
    36121 cggcaagtcc ggcgttgtat ttcagcaacg ttaactgatt cgccaccacc gaatgctgca
    36181 aaatggcatc ctgcggctgc ggttgatatt gcgggcgggc gttatcgatg taggacgggc
    36241 gcagatcgtt aaagttggtg cgcagcgtga cgccaaacat aaaggtgttg ccacgttcat
    36301 agctaaggtt aacgtcggcc caatcggtaa cgcgataaat cgccccgacg ttaaacttac
    36361 ttttttgctc cagcttcccg gcaaaatcct gctgataatt attaccttca tactccagtt
    36421 tcagacgcag cggttgccag ggcgtctggt attccacgcc gccaaacagt gaggcaggac
    36481 cgtggaacat ctggctaccg tcgatagatc ccgcctgttt gtagctgtta tcgcgatagc
    36541 aatatttatc actggctgaa cagagcggat tttttacatt accgctggtg cccagatatc
    36601 cccagcccag gccgagcgta aaatcgaacg gcccccaggc tttgctggca acaagatatt
    36661 ccgcatcaaa cagccccgta ccgccgatat cccgcgcacc aaccgccact tgcggcagcc
    36721 agtaactctc ttcccacaaa cgcagtttga gatcgaaggc tttatcttta tacgtttgat
    36781 cgccagagaa cgcttcgacg ctgctgtact gccgggtgcg cacgtcggtg tagcgcagcg
    36841 ttgtttccag ccacgggaag agttgcactg aagctgagta ataacggtac tgatcgttat
    36901 cgcgatagtt cagactcaac tccccttccc gtgccatacg cgcggtggga gtttgtaata
    36961 atcctacgcc accgaaatcc gactgcgacg gaccaatggg cgcaggatat gtttcgccgt
    37021 agcaagctgt gctaatgccc agcgccagta agctaagcag atgtcttttt ttcattattg
    37081 gggtatccgc tgcgtcagag tctgaagaat gtcggcgtta agggcatcag gcgtctcact
    37141 ccagacggaa tctgcgaggc caacataaat aatgctgccg ggcatcggct ctacgtgacg
    37201 cttgttccag taagccaccg gcgctttttg cgtgcgtccg tccgggtaaa cgacccacgc
    37261 gtagctgcga tccgcaccgc tgagcaggct ctggtcagag agatagctcg ccacgtcgcg
    37321 acctggagtg aatggctgat tgccaggatg gctgataagc ccgaacaacg tgaccgtgga
    37381 cggcggtggt ccgacccaca gcgtgtagtt gccctgcaac ggcgggttac cgcgttcggc
    37441 aacgcggacg atatcgggat caagattgat tttttgcctg cctgtcactt tcaatgcctg
    37501 gatttgctgg cgtaaggcgt taatggcggc ggcatcgtcg gtgctggaat ctgcgccctg
    37561 ttctgccagc cgtgtcagca acgcctgctg ctgacgcaat gctgccgccg ttgccagctc
    37621 ttcgctaatc accgcaccag gccaccagct gtttgccagt cgaggttgcc ctaccagatc
    37681 gattaaatgc tctgcgcccg ttagcgtttt ggcctcactg ctgccattgc tgaacacctt
    37741 gacggttcct gccgcgaaga ccgatgacgc tcccacactc aaaagcaacg cgacaatagt
    37801 ttgtttaatc attgttttgc cgccttgatg agagtggttt tcaccgggaa ataatccgcg
    37861 ccgagatact gttccgactg gcgaatttgc ccttcgctgt cgatccagta gcggttatgc
    37921 cagcgcgcct ggtcggtgga gacttcttcg tccagcacgc gaaccagggt ttcatcgctg
    37981 ccgaccttga cggtatcggt gccatcccat ttgaagactg agcgtgcggt ggcgtagcgt
    38041 acctgctggt actcggtcca gcccatcgtg cgcgtccatg ttgcaccatc aacaatttgt
    38101 gcgggtttaa tcagcgggtc ggcggcaagg ttattcactt caatcaggtt gtcgccgcca
    38161 agcaaagtct tcaccagacg accatgttgt gtgacaagag tggcttgatc ctgagtgacc
    38221 catttttgtt gtccgtcttc agcgaaggcc agcaccacaa acaattgcgg cccgccatta
    38281 agctgcatgt actggctggc gtagggcata ttttgaatat catcgtccgt cagctgtacg
    38341 cctggcgtgc cgaacagact gtcccacagt gaattgccca gctctttagt cgtggctgaa
    38401 caggcctgta atagcaggca gataagaatg agtgcaggtc gcttcacgac tcttctccag
    38461 aggggcagaa taaccacacc ggagtgtggt tatggtaaat cccttcatac ttcaagccac
    38521 agatgcaatg actcaaatta ttttggggat acatacttta ttactgggta ctggtggtcg
    38581 tggttgtgat ggtcccggta ttagaaccat caccgccacc ggttgccgcg agcgcgacac
    38641 ccaccgccga gcttacggtg ctgacgctgg tcgcggagga actccccgcc gaaaccgacg
    38701 ttgctgccga ctctgccgct tcgcccacct gtacaggtgc agcccacgca gaagtcgccg
    38761 caagcgcaga tatggcaaaa atgccataca gaactttttt cataacaatt tcccttcatt
    38821 gaatgaatgg agatttaccc aaaaacattt cgggcgggat cgagtataca caactaaagc
    38881 atgcgaatta cggcgcgggg aattagcgat ggagttttag gataattgga atgaatatca
    38941 aatataagtt tcaacttata tttaaataac aataaaaatc ataagcttaa aataaaaaca
    39001 aaagtaatta atctgcgtat tctccgaaat aaacaacgat taaaaatcta ttttaggatt
    39061 aacctcacgg tatgatttcc gttaaatcac agacaagggg atttatctga taaaacaatg
    39121 ccgacttatt gtcggcattg tttttaaggt tatttaactc ctcatactat gcattaatgt
    39181 cgatattggc atcaggctcc ggttgccgga tgcggcgtaa acgccttatc cggcctacat
    39241 atcggcatca ggcacgcatg atgattaacc gcgccacgct ttatagcggt taatcagacc
    39301 attggtcgag ctatcgtggc tgctgatttc tttatcatct ttcagctctg gcagaatacg
    39361 gtttgccagt tgtttaccca gctccacgcc ccactggtcg aaggtgaaga tgttcaggat
    39421 cacgccctga gtaaagattt tgtgctcata cagcgcaatc aacgcaccca ggctgaacgg
    39481 agtgatttcg cgcagcagga tggagttggt cgggcggtta ccttcgaaca ctttgaacgg
    39541 caccacgtag tcaagcgttg ccggatcttt accctgatcg cgatattcct gctcaaccac
    39601 ttcgcgggat ttaccaaacg ccagcgcttc ggtctgggcg aagaagttag acagcagttt
    39661 ctggtggtga tcagagagcg ggttatgggt gatagccggg gcgatgaaat cgcacggtac
    39721 cattttggtt ccctggtgga tcagctggta gaacgcgtgc tgaccgttag tgcctggttc
    39781 accccagata atcgggccag tctggtaatc cacaacgtta ccgttacggt caacatactt
    39841 accgttggac tccatattgc cctgctggaa gtacgccgcg aaacggtgca tatactggtc
    39901 atacggcaga atcgcttcag tttccgcacc aaagaaattg ttgtaccaga tgccaatcag
    39961 cgccagcagg acaggcaggt ttttctcggc aggcgtggtg gagaaatgct tgtccattgc
    40021 gtgtgcgcca gaaagcagtt caacgaagtt atcaaagccg atggagagaa caatcgacag
    40081 gccaatcgct gaccacaaag agtaacggcc gccgacccag tcccagaact cgaacatgtt
    40141 ggcagtatca ataccaaact cgccaacggc tttggcattg gttgagagcg ccgcaaaatg
    40201 tttcgcaacg tgtttttcat cacctgccgc tttcaggaac cagtcacgcg cgctatgggc
    40261 gttggtcatg gtttcctgag tggtgaaggt tttagatgct accaggaaca gcgtggtttc
    40321 cgggtttact tttttcagca cttccgagat gtgagtccca tcgacgttag aaacaaagtg
    40381 catgttcagg tggtttttgt acggacgcag tgcttcggtc accatgtacg ggccgaggtc
    40441 agaaccgccg atcccgatgt tcactacgtc agtgattgct ttgccggtat aacccttcca
    40501 ctcaccggag ataatcgctt ccgagaagat tttcatcttc tccagcaccg cgttgacttc
    40561 tggcataaca tctttgccat caaccagaat cggggtattg ctacgattac gcagcgctac
    40621 gtgcagcacg gcgcggtttt cagtgcggtt gatcttctcg ccagagaaca tcgacttaat
    40681 cgcgcccgcc agatcgcact ctttcgccag atcctgtaat ttcgccagcg tctcttcagt
    40741 gatgcggttt ttggagtaat ccaccagcat ctgatcgtcg aaggttgcgg agaacttaga
    40801 aaaacgatcg ccgtctttag caaaaagatc ggcgatcgta acgtctttca tttcatcgaa
    40861 gtgtttctgt agtgcctgcc aggcagcggt ctgcgttgga ttgatgtttt tcattagcaa
    40921 tactcttctg attttgagaa ttgtgacttt ggaagattgt agcgccagtc acagaaaaat
    40981 gtgatggttt tagtgccgtt agtgtaatgt tgagtgtaaa cccttagcgc agtgaagcat
    41041 ttattagctg aactactgac cgccaggagt ggatgaaaaa tccgcatgac cccgtcgttg
    41101 acaaccgccc cgctcaccct ttatttataa atgtactacc tgcgctagcg caggccagaa
    41161 gaggcgcgtt gcccaagtaa cggtgttgga ggagccagtc ctgtgataac acctgagggg
    41221 gtgcatcgcc gaggtgattg aacggctggc cacgttcatc atcggctaca ggggctgaat
    41281 cccctgggtt gtcaccagaa gcgttcgcag tcgggcgttt cgcaagtggt ggagcacttc
    41341 tgggtgaaaa tagtagcgaa gtatcgctct gcgcccaccc gtcttccgct cttcccttgt
    41401 gccaaggctg aaaatggatc ccctgacacg aggtagttat gtctgaaatt gttgtctcca
    41461 aatttggcgg taccagcgta gctgattttg acgccatgaa ccgcagcgct gatattgtgc
    41521 tttctgatgc caacgtgcgt ttagtagtcc tctcggcttc tgctggtatc actaatctgc
    41581 tggtcgcttt agcggaaggg ctggaacctg gcgagcgatt cgaaaaactc gacgctattc
    41641 gcaacatcca gtttgccatt ctggaacgtc tgcgttaccc gaacgttatc cgtgaagaga
    41701 ttgaacgtct gctggagaac attactgttc tggcagaagc ggcggcgctg gcaacgtctc
    41761 cggcgctgac agatgagctg gtcagccacg gcgagctgat gtcgaccctg ctgtttgtcg
    41821 aaatcctgcg cgaacgcgat gttcaggcac agtggtttga tgtacgtaaa gtgatgcgta
    41881 ccaacgaccg atttggtcgt gcagagccag atgtagccgc gctggcggaa ctggccgcgc
    41941 tgcagctgct cccacgcctc aatgaaggct tagtgatcac ccagggattt atcggtagcg
    42001 aaaataaagg tcgtacaacg acgcttggcc gtggaggcag cgattatacg gcagccttgc
    42061 tggcggaggc tttacacgca tctcgtgttg atatctggac cgacgtcccg ggcatctaca
    42121 ccaccgatcc acgcgtagtt tccgcagcaa aacgcattga tgaaatcgcg tttgccgaag
    42181 cggcagagat ggcaactttt ggtgcaaaag tactgcatcc ggcaacgttg ctacccgcag
    42241 tacgcagcga tatcccggtc tttgtcggct ccagcaaaga cccacgcgca ggtggtacgc
    42301 tggtgtgcaa taaaactgaa aatccgccgc tgttccgcgc gctggcgctt cgtcgcaatc
    42361 agactctgct cactttgcac agcctgaata tgctgcattc tcgcggcttc ctcgcggaag
    42421 ttttcggcat cctcgcgcgg cataatattt cggtagactt aatcaccacg tcagaagtga
    42481 gcgtggcatt aacccttgat accaccggtt caacctccac tggcgatacg ttgctgacgc
    42541 aatctctgct gatggagctt tccgcactgt gccgggtgga ggtggaagaa ggtctggcgc
    42601 tggtcgcgtt gattggcaat gacctgtcaa aagcctgcgg cgttggcaaa gaggtattcg
    42661 gcgtactgga accgttcaac attcgcatga tttgttacgg cgcatccagc cataacctgt
    42721 gcttcctggt gcccggcgaa gatgccgagc aggtggtgca aaaactgcat agtaatttgt
    42781 ttgagtaaat actgtatggc ctggaagcta tatttcgggc cgtattgatt ttcttgtc
//
LOCUS       DAJTRB010000026        40552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTRB010000026 DAJTRB010000000
VERSION     DAJTRB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.026"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..956)
                     /locus_tag="OO330_001506"
     CDS             complement(<1..956)
                     /locus_tag="OO330_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5882424.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OO330_001507"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OO330_001507"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5882425.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO330_001508"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO330_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO330_001509"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO330_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5882426.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OO330_001510"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OO330_001510"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5882427.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OO330_001511"
     CDS             complement(5018..6346)
                     /locus_tag="OO330_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5882428.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="OO330_001512"
     CDS             complement(6354..8702)
                     /locus_tag="OO330_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5882429.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPSVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="OO330_001513"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="OO330_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5882430.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="OO330_001514"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="OO330_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5882431.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="OO330_001515"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="OO330_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5882432.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="OO330_001516"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="OO330_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5882433.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="OO330_001517"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="OO330_001517"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5882434.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="OO330_001518"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="OO330_001518"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5882435.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="OO330_001519"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="OO330_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5882436.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="OO330_001520"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="OO330_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5882437.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="OO330_001521"
     CDS             18273..19172
                     /locus_tag="OO330_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5882438.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="OO330_001522"
     CDS             19178..21610
                     /locus_tag="OO330_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5882439.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="OO330_001523"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="OO330_001523"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5882440.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="OO330_001524"
     CDS             22723..23988
                     /locus_tag="OO330_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5882441.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="OO330_001525"
     CDS             complement(24229..25821)
                     /locus_tag="OO330_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5882442.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="OO330_001526"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="OO330_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5882443.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="OO330_001527"
     CDS             complement(27018..28136)
                     /locus_tag="OO330_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5882444.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="OO330_001528"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="OO330_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5882445.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="OO330_001529"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="OO330_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5882446.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /gene="opgE"
                     /locus_tag="OO330_001530"
     CDS             29186..30769
                     /gene="opgE"
                     /locus_tag="OO330_001530"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5882447.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="OO330_001531"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="OO330_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5882448.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="OO330_001532"
     CDS             complement(31385..31450)
                     /locus_tag="OO330_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882449.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..32572
                     /gene="rhtA"
                     /locus_tag="OO330_001533"
     CDS             31685..32572
                     /gene="rhtA"
                     /locus_tag="OO330_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5882450.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32871..33374
                     /gene="dps"
                     /locus_tag="OO330_001534"
                     /gene_synonym="pexB"
     CDS             32871..33374
                     /gene="dps"
                     /locus_tag="OO330_001534"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5882451.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33778..34524
                     /gene="glnH"
                     /locus_tag="OO330_001535"
     CDS             33778..34524
                     /gene="glnH"
                     /locus_tag="OO330_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5882452.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34663..35322
                     /gene="glnP"
                     /locus_tag="OO330_001536"
     CDS             34663..35322
                     /gene="glnP"
                     /locus_tag="OO330_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5882453.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35319..36041
                     /gene="glnQ"
                     /locus_tag="OO330_001537"
     CDS             35319..36041
                     /gene="glnQ"
                     /locus_tag="OO330_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5882454.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36302..38526
                     /gene="ybiO"
                     /locus_tag="OO330_001538"
                     /pseudo
     CDS             36302..38526
                     /gene="ybiO"
                     /locus_tag="OO330_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38523..39449)
                     /gene="rlmF"
                     /locus_tag="OO330_001539"
     CDS             complement(38523..39449)
                     /gene="rlmF"
                     /locus_tag="OO330_001539"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5882455.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39725..39985
                     /locus_tag="OO330_001540"
     CDS             39725..39985
                     /locus_tag="OO330_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5882456.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40250..>40552
                     /locus_tag="OO330_001541"
     CDS             40250..>40552
                     /locus_tag="OO330_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5882457.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10102 a        10726 c         9862 g         9862 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accagattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctag tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgctcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgaatctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc gcccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctaactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtactgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc cgtggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca acgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaggtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccacctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    32581 ttctgtatgg ttatgcataa ccatgcagaa tttctcgcta cttttcctct acaccgtatt
    32641 tatatatcga attatgcaaa agcatattta ttccgaaaat tcctggcgag cagataaata
    32701 agaattgttc ttatcaatat atctaactca ttgaatcttt attagttttg tttttcacgc
    32761 ttgttaccac tattagtgtg ataggaacag ccagaatagc ggaacacata gccggtgcta
    32821 tacttagtct cgttaattac tgggacataa catcaagagg atatgaaatt atgagtaccg
    32881 ctaaattagt taaatcaaaa gcgaccaatc tgctttatac ccgcaacgat gtctccgaca
    32941 gcgagaaaaa agcaacagta gagttgctga atcgccaggt tatccagttt attgatcttt
    33001 ctttgattac caaacaagcg cactggaaca tgcgcggcgc taacttcatt gccgtacatg
    33061 aaatgctgga tggcttccgc accgcactga tcgatcatct ggataccatg gcagaacgtg
    33121 ccgtgcagct gggcggtgta gctctgggga ccactcaagt tatcaacagc aaaaccccgc
    33181 tgaaaagtta cccgctggac atccacaacg ttcaggatca cctgaaagaa ctggctgacc
    33241 gttacgcaat cgtcgctaat gacgtacgca aagcgattgg cgaagcgaaa gatgacgaca
    33301 ccgcagatat cctgaccgcc gcgtctcgcg acctggataa attcctgtgg tttatcgagt
    33361 ctaacatcga ataaatccat cgctgatggt gcagaacttt agtacccgat aaaagcggct
    33421 tcctgacagg aggccgtttt gttttgcagc ccacctcaac gcacttattt agtgcatcca
    33481 tctgctatcc ccagctgatt aagtaaattt tttgtatcca catcatcaca caatcgttac
    33541 ataaagattg ttttttcatc aggttttacg ctaaataatc actgtgttga gtgcacaatt
    33601 ttagcgcacc agattggtgc cccagaatgg tgcatcttca gggtattgcc ctataaatcg
    33661 tgcatcacgt ttttgccgca tctcgaaaaa tcaatgagtt gcaaaattgg cacgattttt
    33721 tcatatatgt gaatgtcacg caggggatcg tcccgtggat agaaaaaagg aaatgctatg
    33781 aagtctgtat taaaagtttc actggctgca ctgaccctgg cttttgcggt ttcttctcat
    33841 gccgcggata aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt tgaatttaaa
    33901 cagggcgata aatatgtggg ctttgacgtt gatctgtggg ctgccatcgc taaagagctg
    33961 aagctggatt acgaactgaa gccgatggat ttcagtggga tcattccggc actgcaaacc
    34021 aaaaacgtcg atctggcgct ggcgggcatt accatcaccg acgagcgtaa aaaagcgatc
    34081 gatttctctg acggctacta caaaagcggc ctgttagtga tggtgaaagc taacaataac
    34141 gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg ctgtgaagag cggtactggc
    34201 tccgttgatt acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt cccgaacatc
    34261 gataacgcct atatggaact gggcaccaac cgcgcagacg ccgttctgca cgatacgcca
    34321 aacattctgt acttcatcaa aaccgccggt aacggtcagt tcaaagcggt aggtgactct
    34381 ctggaagcgc agcaatacgg tattgcgttc ccgaaaggta gcgacgagct gcgtgacaaa
    34441 gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt acaacgaaat ctacaaaaaa
    34501 tggttcagta ctgaaccgaa ataataacgc tacacctgta aagcgcactg gcagttccct
    34561 ctcccccctg gggagaggat tagggtgagg ggcgcaaacc cgctccgggg ccattaatta
    34621 ccctgaattt gattatttac accacggtaa caggaacaac atatgcagtt tgactggagt
    34681 gccatctggc ctgccattcc gcttctgatt gaaggtgcca aaatgaccct gtggatttcg
    34741 gtcctcggtc tggctggcgg tctggtaatc ggattgctgg caggttttgc acgcaccttc
    34801 ggtggttgga tagccaacca cgtcgcgctg gtctttattg aagtgatccg cggcacacct
    34861 atcgtcgtcc aggtgatgtt tatctatttc gccctgccga tggcgtttaa cgacttacgc
    34921 atcgacccat ttactgcggc ggtggtcacc atcatgatca actccggcgc gtacattgcg
    34981 gaaatcacgc gtggtgcggt gctgtctatc cacaaaggtt ttcgtgaagc aggactggcg
    35041 ctcggtcttt cacgttggga aaccattcgc tacgtcattt taccgctggc actgcgtcgt
    35101 atgctgccgc cgctgggtaa ccagtggatc atcagcatta aagacacctc gctgtttatt
    35161 gttatcggcg tggcggaact gacccgtcag gggcaagaaa ttattgccgg taacttccgc
    35221 gcccttgaga tctggagcgc cgtggcggtg ttctatctga ttattaccct ggtgctgagc
    35281 tttattctgc gtcgtctgga aagaaggatg aaaatcctgt gattgaattt aaaaacgtct
    35341 ccaagcactt tggcccaacc caggtgctgc acaatatcga tttgaacatt gcccagggcg
    35401 aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc gaccctgctg cgctgcatca
    35461 acaaactgga agaaatcacc tccggcgatc tgattgtcga tggcctgaag gtcaacgatc
    35521 cgaaagttga cgagcgcctg attcgccagg aagcaggtat ggtgttccag cagttttacc
    35581 tcttcccgca tctgacggcg ctgggaaacg tcatgtttgg tccgctacgc gtgcgtggcg
    35641 cgaacaaaga agaggcggaa aaactggcgc gtgagctgct ggcgaaagtc ggtctggcag
    35701 aacgtgcaca tcactaccct tccgaacttt ctggtggtca acagcagcgt gtggcgattg
    35761 cccgcgcgct ggcggtgaag ccgaaaatga tgctgtttga tgaaccgact tccgctcttg
    35821 acccggaact gcgccatgaa gtgctgaagg ttatgcagga tctggctgaa gaagggatga
    35881 cgatggtgat cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg cggctgatct
    35941 ttatcgacaa aggccggatt gcggaagatg gcaatccgca ggtgttgatc aagaacccgc
    36001 cgagccagcg cttgcaggaa tttttgcagc acgtctctta ataagacaca ttgcctgatg
    36061 cgctacgctt atcaggccta caggatatct ggcaacttat taaaattgca tgaacttgta
    36121 ggacggataa ggcgttcacg ccgcatccgg caaaaaagcc cgcacgttgt cagcaacctg
    36181 cttaatatcc cttcctccct ttcacccgaa agggaggcac accagattcc tctcatttaa
    36241 aatcgcccct cccccagcat ctatacttat ctttttgctc tgttttctca ctggaggagt
    36301 catgcggtgg atcctgttca tcctcttctg cctgctgggc gcacctgccc acgcggtatc
    36361 catacccggc gttacaacca caacgacaac ggactcaacg actgaaccgg ccccggaacc
    36421 ggatatcgaa caaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc
    36481 tcgcgtaaag agttgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg
    36541 gtcccgaaga tcgtgccgcc gacactggtt gaagagcaaa ccgtgctgca aaaggtcacc
    36601 gaagtcagcc gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat
    36661 atcaccggct ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat
    36721 ttttcaatgt tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg
    36781 ccgctatatc gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg
    36841 ttgcagcttc cggcgatgat tattggggcg tttattatcg acctgctgtt actggccctg
    36901 acattatttg tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct
    36961 ttccaacaaa gtttgtttct caacgccttt gccctgattg aatttttcaa agccgtacta
    37021 cgcctgattt tttgcccaaa cgtggcggag ctgcgcccgt tcacgattca ggacgagagc
    37081 gcccgttact ggggtcgtcg cctgagctgg ttaagcagcc tgattggcta tggcctgatt
    37141 gtggccgtgc cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc
    37201 atcattatgc tgtgcatgac cgtctgggcg ttgtacctga tcttccgaaa taaaaaagag
    37261 attacccagc atatgctcaa ctttgcggag cattcgctgg cttttttcag cctgtttatc
    37321 cgcgcctttg cgctggtgtg gcactggctg gcaagcgcct attttatcat gctgtttttc
    37381 ttttcgttgt tcgatccggg caacagtctg aaatttatga tgggtgcaac ggtgcgcagc
    37441 ctggcgatta ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa
    37501 accatcaccc tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat
    37561 ggctggcttt cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg
    37621 ttgctgttga gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg
    37681 cagaaaaccg tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc
    37741 tggacggtgc tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg
    37801 ctacccagcg cccgtacgcg taccctgctg acgcggttcc gtaacgcgct ggcggtgatt
    37861 atcagtacca tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg
    37921 ctggcaggtg ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtaaaa
    37981 gatattatca ccggggtatt tattcagttt gaaaacggca tgaacactgg agatttggtg
    38041 actatcgggc cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc
    38101 caggataccg gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc
    38161 gtccgcggta ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat
    38221 aaagccaatc aggcactgaa agatgcggta acggaattga tggaaaacga agaaattcgc
    38281 gggctgatta ttggtgaacc gaactttgcc gggattgtcg gcttaagcaa taccgcgttt
    38341 acactgcatg tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc
    38401 gacagccagg tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat
    38461 caggtgctgc ctgctccggg cgcgaccccg gctgaaccgc tgccgccggg ggagccaacg
    38521 ctttaacgtt ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca
    38581 ataaagcgac tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc
    38641 acgtcggtca gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta
    38701 aaccacatca cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaagttg
    38761 acttcaccgc cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct
    38821 ttgttcagcc ccaggttacg gcgtttacgc tcactacctg cccgcgccgc agcggcggaa
    38881 tcgtggaatg gcgggttaca caaggtcgcg tcgtattgct cgtttttgtg gatgatgccg
    38941 ttaaaaatcg ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga
    39001 ttagcactga taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta
    39061 aaacgccagc catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg
    39121 atatccagaa tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca
    39181 aggtgatgaa tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata
    39241 tcccagttcg ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc
    39301 agcggattgg caaagtctac gctttgctcc cctgcgggtg taagcgtgag gaattgcctg
    39361 agttcaggat tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc
    39421 ggatgcaacc ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata
    39481 cccgcataac gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca
    39541 tggctatggt ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc
    39601 ttattttgta aaaatatatc gttcaaaaaa taagcatatc tctgtcaatt ttttgcgtca
    39661 tttgcatcgc gcaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt
    39721 cattatgaaa aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac
    39781 ggcttatgcc gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac
    39841 cgtttcggca actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc
    39901 gcgcgaacaa ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt
    39961 aggtactgca accatctaca aataacccca ccaacgtaac tgcattcgcc tctggatgca
    40021 gttttcgcct gttttgtaat caaaaaacat caaatatgaa cgcaatgtaa ataaatgtat
    40081 ttctttttcg cgcaatgggt gatagaaaat cgcatcaagt gataatgctt atcaaaatta
    40141 ttatcacttg cacgaacact atcacgggat taacagtggc atcgcatccg cagagatgct
    40201 ttctcgtggc agtgaaaatt tcaacatata agaaaaagtc acctgcaaaa tggaaaacaa
    40261 tcgcaatttc cctgccagac aatttcattc gctcacgttc tttgccggtc tttgtattgg
    40321 tattacgcct gtggcacagg cactcgccgc cgaagggcaa gctaacgcgg atgacacgct
    40381 ggttgtcgaa gcatcaacgc cttcgcttta tgcgccacaa caatctgccg atccgaaatt
    40441 ctcgcgtccg gtagcggata ctacccgcac gatgacggtg atttctgaac aagtgattaa
    40501 agatcagggc gcaaccaacc ttaccgacgc gctcaaaaac gtccccggcg tg
//
LOCUS       DAJTRB010000027        40514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTRB010000027 DAJTRB010000000
VERSION     DAJTRB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.027"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..176)
                     /locus_tag="OO330_001542"
     CDS             complement(<1..176)
                     /locus_tag="OO330_001542"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5882458.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAE"
     gene            complement(317..2350)
                     /gene="metG"
                     /locus_tag="OO330_001543"
     CDS             complement(317..2350)
                     /gene="metG"
                     /locus_tag="OO330_001543"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5882459.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2482..3591
                     /gene="apbC"
                     /locus_tag="OO330_001544"
     CDS             2482..3591
                     /gene="apbC"
                     /locus_tag="OO330_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5882460.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3853..4134
                     /locus_tag="OO330_001545"
     CDS             3853..4134
                     /locus_tag="OO330_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5882461.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4427..4951
                     /locus_tag="OO330_001546"
     CDS             4427..4951
                     /locus_tag="OO330_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5882462.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKVIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5032..5706
                     /locus_tag="OO330_001547"
     CDS             5032..5706
                     /locus_tag="OO330_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5882463.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5722..8202
                     /locus_tag="OO330_001548"
     CDS             5722..8202
                     /locus_tag="OO330_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5882464.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGSIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8218..9251
                     /locus_tag="OO330_001549"
                     /pseudo
     CDS             8218..9251
                     /locus_tag="OO330_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9333..9671)
                     /gene="rcnB"
                     /locus_tag="OO330_001550"
     CDS             complement(9333..9671)
                     /gene="rcnB"
                     /locus_tag="OO330_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5882465.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9890..10729)
                     /gene="rcnA"
                     /locus_tag="OO330_001551"
                     /pseudo
     CDS             complement(9890..10729)
                     /gene="rcnA"
                     /locus_tag="OO330_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10851..11123
                     /gene="rcnR"
                     /locus_tag="OO330_001552"
                     /pseudo
     CDS             10851..11123
                     /gene="rcnR"
                     /locus_tag="OO330_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      11215..11311
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11346..12134
                     /gene="thiM"
                     /locus_tag="OO330_001553"
     CDS             11346..12134
                     /gene="thiM"
                     /locus_tag="OO330_001553"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5882466.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12131..12931
                     /gene="thiD"
                     /locus_tag="OO330_001554"
     CDS             12131..12931
                     /gene="thiD"
                     /locus_tag="OO330_001554"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5882467.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            12996..13817
                     /locus_tag="OO330_001555"
     CDS             12996..13817
                     /locus_tag="OO330_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5882468.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13865..14611
                     /locus_tag="OO330_001556"
     CDS             13865..14611
                     /locus_tag="OO330_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5882469.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14585..15550)
                     /locus_tag="OO330_001557"
     CDS             complement(14585..15550)
                     /locus_tag="OO330_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5882470.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15547..16550)
                     /locus_tag="OO330_001558"
                     /pseudo
     CDS             complement(15547..16550)
                     /locus_tag="OO330_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16547..17824)
                     /locus_tag="OO330_001559"
     CDS             complement(16547..17824)
                     /locus_tag="OO330_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5882471.1"
                     /translation="MKTTAKLSLMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18081..19133
                     /gene="fbaB"
                     /locus_tag="OO330_001560"
     CDS             18081..19133
                     /gene="fbaB"
                     /locus_tag="OO330_001560"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5882472.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19441..20295
                     /gene="gatY"
                     /locus_tag="OO330_001561"
     CDS             19441..20295
                     /gene="gatY"
                     /locus_tag="OO330_001561"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5882473.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20324..21586
                     /gene="gatZ"
                     /locus_tag="OO330_001562"
     CDS             20324..21586
                     /gene="gatZ"
                     /locus_tag="OO330_001562"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5882474.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21596..22048
                     /gene="gatA"
                     /locus_tag="OO330_001563"
     CDS             21596..22048
                     /gene="gatA"
                     /locus_tag="OO330_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5882475.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22079..22363
                     /gene="gatB"
                     /locus_tag="OO330_001564"
     CDS             22079..22363
                     /gene="gatB"
                     /locus_tag="OO330_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5882476.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22367..23722
                     /locus_tag="OO330_001565"
     CDS             22367..23722
                     /locus_tag="OO330_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5882477.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23770..24810
                     /gene="gatD"
                     /locus_tag="OO330_001566"
     CDS             23770..24810
                     /gene="gatD"
                     /locus_tag="OO330_001566"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5882478.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24916..25689
                     /locus_tag="OO330_001567"
     CDS             24916..25689
                     /locus_tag="OO330_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5882479.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25771..26670)
                     /gene="yegS"
                     /locus_tag="OO330_001568"
     CDS             complement(25771..26670)
                     /gene="yegS"
                     /locus_tag="OO330_001568"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5882480.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27076..27393
                     /locus_tag="OO330_001569"
     CDS             27076..27393
                     /locus_tag="OO330_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882481.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27735..29096)
                     /gene="yegQ"
                     /locus_tag="OO330_001570"
     CDS             complement(27735..29096)
                     /gene="yegQ"
                     /locus_tag="OO330_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5882482.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29243..29574)
                     /locus_tag="OO330_001571"
                     /pseudo
     CDS             complement(29243..29574)
                     /locus_tag="OO330_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(29765..30487)
                     /gene="baeR"
                     /locus_tag="OO330_001572"
     CDS             complement(29765..30487)
                     /gene="baeR"
                     /locus_tag="OO330_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5882483.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(30484..31887)
                     /gene="baeS"
                     /locus_tag="OO330_001573"
     CDS             complement(30484..31887)
                     /gene="baeS"
                     /locus_tag="OO330_001573"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5882484.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(31884..33299)
                     /locus_tag="OO330_001574"
     CDS             complement(31884..33299)
                     /locus_tag="OO330_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5882485.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(33300..36365)
                     /gene="mdtC"
                     /locus_tag="OO330_001575"
     CDS             complement(33300..36365)
                     /gene="mdtC"
                     /locus_tag="OO330_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5882486.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(36366..39488)
                     /gene="mdtB"
                     /locus_tag="OO330_001576"
                     /pseudo
     CDS             complement(36366..39488)
                     /gene="mdtB"
                     /locus_tag="OO330_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(39488..>40514)
                     /gene="mdtA"
                     /locus_tag="OO330_001577"
     CDS             complement(39488..>40514)
                     /gene="mdtA"
                     /locus_tag="OO330_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5882487.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
BASE COUNT        10210 a         9940 c        10475 g         9889 t
ORIGIN      
        1 ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga aaaacttttt
       61 atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc gtttccctgt
      121 tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg tctcattctg
      181 catccctgta attttatcca gttgaatgca gatgctacca gtatttatac gggttagaga
      241 gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc tgtcgatgca
      301 gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca tccgggctta
      361 gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca gagataccga
      421 agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca atcagtgcct
      481 gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca ccgccgagat
      541 ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt tcgatcagcg
      601 ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga atcggatcat
      661 ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta gaggcttcca
      721 ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac ggattcactt
      781 tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg aggaaggctt
      841 ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta agtcatcagc acgcggaaca
      901 ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc tgttttgcga
      961 ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt gccatgattt
     1021 cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact tcagcggcat
     1081 cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca ccgtcaaaac
     1141 gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc acgatatcgg
     1201 cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg cgcgaagaga
     1261 gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc caggtgctgg
     1321 ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc acatagccat
     1381 gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca ggccagaaca
     1441 ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg gtggagtctt
     1501 tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga ttcttgaaag
     1561 aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc ggtgcgttcg
     1621 gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc aggccggatt
     1681 caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg cgggtccatg
     1741 cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa tcacgcatca
     1801 ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg ctgtaggtcg
     1861 cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc gggcaggtgc
     1921 ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc tgagagatgg
     1981 tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct gacaactggc
     2041 ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag cctgcgaaat
     2101 cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata ccgagctgct
     2161 gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag ttgacctcgt
     2221 ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc agcatatggc
     2281 cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt ttcttcgcga
     2341 cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag taccaaatgg
     2401 gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct gatggtttat
     2461 ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc agaagccttg
     2521 cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa gcataacctc
     2581 accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca tgtggaactg
     2641 gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg tagcgccgaa
     2701 ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa catagccact
     2761 ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat cgcggtgagt
     2821 tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct ggcactggcg
     2881 gcggaagggg cgaaagtcgg cattttggac gccgatatct acggcccatc aattcccacc
     2941 atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat ggcaccgatt
     3001 atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga caatgcgatg
     3061 gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga tgttgcagga aaccttgtgg
     3121 ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat tcaattgacg
     3181 ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca ggacatcgcg
     3241 ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc ggtgctcggt
     3301 attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga accgattttc
     3361 ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct ggggcagatg
     3421 ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt tatcagccgt
     3481 ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc agctcagctc
     3541 tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta acgcctatta
     3601 accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt gtatttgttt
     3661 ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct tcgtccgaaa
     3721 ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg tgctgtgtag
     3781 tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc tttactacag
     3841 ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc ctatggattg
     3901 gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag gatcagtccc
     3961 ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg tcagttaacg
     4021 tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac gacagaagtg
     4081 gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga ttaatttggc
     4141 aacctgattt taattgcatt tagaaagacg aaatcattta tataaatgat ttcgtctttt
     4201 ttattgatat caacaatacc atttacataa atctacatta attaatagat gtgataaata
     4261 tttaatattc ttaagtattt gttgtattta tattgcattg gttaatttgt tttaaatcaa
     4321 taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt ttccaagaga
     4381 tacagactct taacaaccaa aaataaattt tattaaggaa catattatga aacgttcaat
     4441 tattgctgcc gctgtctttt cttctttttt tatgagcgct ggagtatttg ctgcagacgt
     4501 tgataccgga acattaacca ttaaaggaaa tattgcagaa tctccgtgta aattcgaagc
     4561 gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct ttgaaggtaa
     4621 agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt taaaaattag
     4681 ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca aaataaccgg
     4741 taacgataag gtgatagcca gtagcaacga taccgtaggt gataacagcg atgtcctaga
     4801 tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat atgctattcc
     4861 gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg atgtgttatc
     4921 ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt ctccaggtaa
     4981 taaacatggc cgctatcccc atggcggctt tttaatttaa gaggcattaa gatgaaagga
     5041 ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat tgttatctac
     5101 gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt gatgaaccag
     5161 ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc gttaccacca
     5221 gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg ggcaaattcc
     5281 gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa agaaagcatt
     5341 ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg caagaatgca
     5401 ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg tattgctccg
     5461 gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg attggtgata
     5521 aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa tgttaaagtc
     5581 aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat caaaagtaat
     5641 aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat tagtgacaaa
     5701 atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc aatcgtagcg
     5761 ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca ttttatgata
     5821 ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca acccttaccg
     5881 gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata tgagattatt
     5941 gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg gttaggcatt
     6001 aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact tgttcagggt
     6061 gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc gcaggcttgg
     6121 gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg tattaatgcg
     6181 ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc gggtaacaac
     6241 aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca actgcattct
     6301 gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa taccctgtat
     6361 ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat gtacacatca
     6421 agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga tatgcagatg
     6481 ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca gagtaacgcg
     6541 ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc tcctggcccg
     6601 tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt tagcgtgaaa
     6661 gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc aaatatgctg
     6721 caacccggcg tgtcgaaata tgattttgcg gcgggccgta gccatattga aggggcgagc
     6781 aagcaaagtg attttgtcca ggcaggttat cagtatggtt ttaataactt attgacgctg
     6841 tatggcggca cgatggttgc taataattac tatgcgttta ccctcggaac gggttggaac
     6901 acgcgcattg gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca agacaacggc
     6961 gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag ccaaacgtcg
     7021 acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg attaccggac atttaacgat
     7081 cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatat ctatgacatt
     7141 gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa tatgagccag
     7201 tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga ttactggggg
     7261 cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg gcggataagc
     7321 tataccctcg cggcaagcca ggcgtatggc gagaatcatc atgaagagaa acgttttaat
     7381 atttttatat caattccctg tgattggggt gatgacgtta cgacgcctcg tcggcaaata
     7441 tatatgtcta actcaacgac gtttgatgat cagggttttg cctcaaataa tacgggatta
     7501 tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag tcatcaacat
     7561 cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt tgcgacagtg
     7621 aatggcagtt atagtcagtc gagtacttat cgacagactg gagccagtgt ttcagggagc
     7681 attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac gtttgctgtg
     7741 atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg cacaacaaac
     7801 cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca cctgatgctg
     7861 gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat tgccgcccct
     7921 tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc ctggtttata
     7981 aaagcgttaa gagcggatgg tcaaccatta acgtttggtt atgaagtcaa tgatatccat
     8041 ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac caatgaaata
     8101 ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac aatcaccttc
     8161 ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg tggttctatg
     8221 gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag ctatgcggca
     8281 tgttatagtg agttatctgt tcagcacaat ttggttgttc aggggaattt tgcacttact
     8341 caaacacaaa tagcgacata tgagcataat tttaatgatt cgtcatgcgt gagtacaaat
     8401 actatcaccc ctatgagccc atcggatatt attgttggac tttataacga taccattaaa
     8461 ttaaatttac attttgaatg gaccaataaa aacaacatca cgttgtcaaa taatcagagc
     8521 agtttcacca gtggttattc ggtaacggtg acacctgcgg ccagtaatgc aaaagtgaat
     8581 atttctgcgg ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt atccagtgct
     8641 tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt aggtggcaag
     8701 tcatggggtg catgtgtaaa tagctacaga aatgcattgg cacaaaatgc aggtgtctat
     8761 tccttcaatc tgacattgtc atacaacccg ataaccacaa cctgcaaaca ggacgattta
     8821 ttaattactt tagacagtat tcccgtttca caattaccag ccacaggtaa caaagcaaca
     8881 ataaatagta aaaaaaggga tattattctg cgttgtaaaa atttattagg tcaacaaaat
     8941 caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac caacagcaac
     9001 acaatactga aaggtgcgga agataatgcg taggatttat tcttgaaagt aatggttcgc
     9061 cagtcacact tttaaatatc actaacagca gtaaaggata cacaaattta aaagaaattg
     9121 cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc agttactacg
     9181 tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta atcaacgtga
     9241 aatacgacta ataacaagca aaacgagcaa gtggctaata ataaaaaata acaaggtgtc
     9301 tgcatttccc cctcatgatg agggggcttt ttttagcgat gataaaaaat ctcaccgtcg
     9361 taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc acccatatag
     9421 gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg cttaatgttg
     9481 tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg cttaaagtca
     9541 gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga acccgcggtg
     9601 gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa catcttattt
     9661 ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata tctttcctgt
     9721 catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg taaaaagtgt
     9781 aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga aatagctgat
     9841 ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt tatcgcatta
     9901 tgcccatgaa gccatgtacg cccatataca caccgactaa gccaatcaac agactggaaa
     9961 aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg acctgctgaa
    10021 cgctgattgc tgcgccaacg cctacggtga caagcgttaa cgccaggcca atgctgaaac
    10081 tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc aacagcacgg
    10141 taattgctgc cgggcagggg ataaagccac cggttaagcc aaataacaaa atttgccagt
    10201 tgatgacctc tctaccatca aagcgtcgtt taatgtcatt ggcatgggct cgtgcatggg
    10261 catcctgata ctcgccatgt tcgtgatggt gatgatgtcc ttggtcgtgg tgatgttcgt
    10321 ggtcttgatg atgttcgtga tcgtgatgat gctcataatc atgcccgtgc atattctcca
    10381 gccagttgcg ttcgccgcgc taggtacgcc agaacatcca gaacgcggtg ccaataatga
    10441 tcactgcgga aatcagctgg agccacggtt ctgctgattg agcagtaaag cgcttactga
    10501 tcaccatccc gccaaaggca atcaaccaga ccaccgcggt atgcgaaatg gttgctgcca
    10561 gtccgagcat caccgcctgt ttaatggtgc ctttgatggc gatgataaac gccgccatca
    10621 tcgtttttga gtgccctggt tccaggccat gaagcgcacc aagtaagatg gcgctgggga
    10681 tgaagaacca ggcgtttcct tgctgaagaa gagttgtaaa ttcggtcatg ataataattc
    10741 ttagtattaa ttcggcaatc tgattctact cccccccagt acctgatact acccccccag
    10801 tagattaata gtgctatgat ttttcatatt cttgttaacc aggtgttgcc atgtctcata
    10861 caatccgtga taaacagaaa ctgaaagcgc gtgccagtaa gattcagggc caggtcgtgg
    10921 cgctcaagaa aatgctcgac gagccgcacg aatgcgctgc agttttacaa cagattgctg
    10981 ctatccgtgg agcggtaaac ggtctgatgc gggaagtgat taaaggtcat ctgacggaac
    11041 acatcgttca ctagggggat gagctaaaac gtgaagaaga tctggatgtc gttctgaagg
    11101 tgctggattc atatatcaaa taatttatta acgcgattgt aaaactgccg tttttcctcg
    11161 tttacaacgg gtgcgctgga cattaccatc ctcctctgcg atttatcatc gcaaccaaac
    11221 gactcggggt gcccttctgc gtgaaggctg agaaataccc gtatcacctg atctggataa
    11281 tgccagcgta gggaagtcac ggaccaccag gtcattgctt cttcacgtta tggcaggagc
    11341 aaactatgca agtcgacctg ctgagttcag cgcaatctgc gcacgcgtta cacctttttc
    11401 accaacattc ccctcttgtg cactgcatga ccaatgatgt ggtgcaaacc tttaccgcca
    11461 ataccttgct ggcgctcggt gcatcgccag cgatggttat cgaaaccgaa gaggccagtc
    11521 agtttgcggc tatcgccagt gccttgttga ttaacgttgg cacactgacg cagccacgcg
    11581 cacaggcgat gcgtgctgcc gttgagcaag caaaaagctc tcaaacacgc tggacgcttg
    11641 atccagtagc ggttggtgcg ctcgattatc gccgccattt ttgtcatgaa cttttatctt
    11701 ttaaaccggc agcgatacgt ggtaatgctt cggaaatcat ggcattagct ggcattgcta
    11761 atggcggacg gggagtggat accactgacg ccgcagctaa cgcgataccc gctgcacaaa
    11821 cactggcacg ggaaactggc gcaatcgtcg tggtcactgg cgaggtggat tatgttaccg
    11881 atggacatcg tatcattggt attcacggtg gcgatccgtt aatgaccaaa gtggtaggaa
    11941 ctggctgtgc attatcggcg gttgtcgctg cctgctgtgc gttaccaggc gatacgctgg
    12001 aaaatgtcgc atctgcctgt cactggatga aacaagccgg agaacgcgca gtcgccagaa
    12061 gcgaggggcc aggcagtttt gttccacatt tccttgatgc gctctggcaa ttgacgcagg
    12121 aggtgcaggc atgaaacgaa ttaacgctct gacgattgcc ggtactgatc cgagtggtgg
    12181 tgcggggatt caggccgatc ttaaaacctt ctcggcactt ggcgcttatg gttgctcagt
    12241 tattactgca ctggtggcgc aaaatacccg tggcgtacag tcggtgtatc gcattgagcc
    12301 tgattttgtc gccgcccagc tcgattcggt gttcagcgat gtgcgaatcg ataccactaa
    12361 aatcggtatg ttggcggaaa ccgatattgt tgaagcggtg gcagaacggt tgcaacgtta
    12421 tcagatccaa aacgtggtac tcgacaccgt tatgctggca aaaagcggcg acccgctgct
    12481 ttcaccttcg gcggttgcta cgctgcgcag tcgattattg ccacaggttt cattaataac
    12541 gccaaacttg cccgaagctg ccgccttgct cgacgcgcca cacgcgcgca ccgaacagga
    12601 aatgctggaa caagggcgat cgctgttggc gatgggctgt ggcgcagtgc taatgaaagg
    12661 tggtcatctg gatgatgagc aaagcccgga ctggctgttt acccgcgagg gtgaacaacg
    12721 gtttaccgca ccgcgcatta tgaccaaaaa cacccacggc actggttgta cactctctgc
    12781 ggcgttggct gcactacgcc cgcgccatac aaactgggct gacaccgtac aggaggcaaa
    12841 aatctggctt tcatcggcgt tagcccaggc cgacacgctg gaagttggtc acggtattgg
    12901 tccggttcac cacttccacg cctggtggtg agcaccgtac tatagctgct ttggttattg
    12961 tcagcctttg gcactgacat tcagggatga aaccaatgca gctaagaatt accagtcgaa
    13021 aaaaactcac cgctctatta tgcgctcttg ggctaatttc catcgttgca atttatccgc
    13081 gtcagacggt gaattttttc tattcgacag cagttcagat tacagactac atccacttct
    13141 acggttatcg cccggttaaa tctttcgcta ttcgcattcc tgccagttac accattcacg
    13201 gattagatgt ttcgcgctgg caggagcgta tcgactggca gcgggtggca aaaatgcgcg
    13261 acaacgatat ccgcttacag tttgctttta ttaaggctac ggaaggcgaa aagctggtgg
    13321 acccctattt ttcgcgtaac tggcaattaa gccgcgaaaa tggcctgctg cgcggggcgt
    13381 atcattattt ctccccgtcg gtatccgctt cagttcaggc gcgattattt ctgcaaacgg
    13441 tggatttctc acaaggcgat ttccctgccg tgctggacgt agaagaacgg ggaaaattat
    13501 cggcaaaaga attacgcaag cgggtaagtc agtggctaaa aatggtcgaa aaacgcacgg
    13561 gaaaaaagcc gattatttac tcaggagccg ttttttatca caccaatctg gcgggctatt
    13621 tcaatgaata tccgtggtgg gtggctcact attatcaacg tcgtccggac aatgacggca
    13681 tggcctggcg cttctggcag cattccgacc gtggacaggt agatggcatc aatggtccgg
    13741 tggattttaa tgtgtttaat ggcacgggga tgagttgcag gcattcgttg atgggattaa
    13801 agaaacgcct taaataagaa gcagtgatat actcccacca gactccctgg caggaaaaag
    13861 acaaatggaa caagcgcata cccagctgat tgcccaactg aacgagcgta ttttagcggc
    13921 ggataacacg ccgctttata ttaagtttgc cgaaacggta aaaaatgccg tgcgcagcgg
    13981 ggtgctggag catggcaata ttttgcccgg tgagcgtgat ttaagtcagt taaccggcgt
    14041 ttcgcgcatt acggtgcgca aggcgatgca ggcgctggaa gaagagggcg tggtgacgcg
    14101 ttcgcgcggt tacggcacgc agatcaacaa catcttcgaa tactcgttga aagaagcgag
    14161 aggtttttct cagcaggtgg tattgcgcgg gaaaaagccc gatacgctat gggttaataa
    14221 gcgtgtcgtg aaatgtcccg aagaagttgc gcagcagctg gcggtcgaag cgggaagtga
    14281 tgtctttttg cttaagcgta ttcgctatgt cgatgaagaa gcggtatcga ttgaggaatc
    14341 gtgggttccg gcgcatttaa ttcatgatgt tgatgccatc ggaatttctc tttatgacta
    14401 tttccgcagc cagcacattt acccacaacg tacgcgttcc cgcgttagcg cccggatgcc
    14461 ggatgccgag tttcagtcac atattcagtt agatagcaaa ataccggtgc tggtgatcaa
    14521 gcaagtggcg cttgaccaac agcaacggcc tattgagtac agcattagcc actgtcgcag
    14581 cgatctatac gtttttgtgt gcgaggagta gttcctcgcg cgttggcgca caatcaccgc
    14641 cccgatgccc gacaacccac gacgccactg cattgcccag taatacggca tccgccagtg
    14701 gcagaccaga ggccagcccg gcaagtacgc cacctgcatg actgtcgccc gccccaatgg
    14761 tgtctacaac ttgcgttgga aatgccggaa tgcagccaga agcgtcgttg ctgaaatacc
    14821 atgcgccttc tttatcgaga cgaacgatca acggcgcggc aaatttctcc tgccattgct
    14881 tgccaagtgt tgttatctct gcggataaag caaaacgttc ggcggcaatc tcagcctctt
    14941 gacgattgag tgacactaaa ggtcgacagg ccatgatccg cgccagtaat gcgtccggga
    15001 tatcgccaat acgtgggcaa aaatcgataa acggcgtcac gtcttgcagc ttttccagcc
    15061 attccactaa caattcgccg cagggcgagg ccagttgata accggaaaaa tagagcaggt
    15121 tgccaggcgc aacggttaat cgtgccaacc attggcgatt ccattgattt tccacaccgc
    15181 tgaatgacat aaaagtgcgt tcgccatccg gctcaaccag tgccagacac cagccgttat
    15241 cgctttcggc gttatcgatc aggctgatta acccctcttt cgccatccgg ttgcgaataa
    15301 tctccgccca cacgccctga ccgagcggca aggcattacc cgcttcgatg ccgaggcgct
    15361 ttaacgccac ggcaatattc agtgcgcagc cgccaacgtt aacgctctgc tgtttcagtt
    15421 cgatatcgca cccacgccag gggagggcat aagcgtcggc gatcacgtca atgaccgccg
    15481 cgccgacgac catcaccggc tgacgcgtgg ttaattcagg cagcagtgtg tgtaatcgag
    15541 cgccgctcat accgcctccc gttgttgacg atatttcgcc agcgctgtgg catagcggtt
    15601 gaaatcaagc tgatttaccg catccagctc cgcctttaac gcaggatcga cagcgttaat
    15661 gccatgcaac gcgccgcaaa tcgccgtcgc catagcacct atggtgtctg tgtcgccgcc
    15721 aagattggcg cacaggacgg cgcagcggtt tgggtcggtt tgtgccagtt caactagcgc
    15781 aatggcgcac ggaacggact caatagtgct ggtgcctgcg ccaacgacct ggtaaagctg
    15841 ttcgctggcg gattcggtgc cgtcggcatt acgcacaatt ttcagcgcaa tctccagacg
    15901 tgccgccagt gaggcgctga acgtggtggt gcgtttttgc tgtgcatgtc gcgcaattga
    15961 aggcaatgaa tcaacaatcg ctgaccagcg ttctccgtca atggcacgag aaatcgccca
    16021 tgcgatgact accgcgcctg caaccgccag atcggattta tgggtcgggc tggacgccag
    16081 cgccacatca tcaataaagg aatcaacatc acgcgctgga agcaaacaac ctaatggcga
    16141 gacgcgcatc gctgcgccgt tggttacgcc gttgttttcc agttcagcaa cgggtttacc
    16201 gtcgcgaatg gcgttaagcg caatcttcga ggtcggacct agtacgtttt tgttaaaggc
    16261 gtcgaaacgc aacgcccagt cgagaatatt acgcccaatc agattcggat cgatcttgcc
    16321 ttcacattcc agtaacgcat ccgccagaca caggccatcg aggtatcgtc ggtgaattcg
    16381 gcgcggttaa aataacaggc cgcgttattc tcctttggtc caggcagaaa acggtcaatc
    16441 cagccaaagt gtgctttaac gcggctgcgt gtccaaagct cggagggcat ccccatcgca
    16501 tcccctaacg cctgcccata aagagcaccg agaatacgtt ctgttttcat ttaacttccc
    16561 cttgtgtcaa cgcaatatct cgatcatcga ccttgatagc cgtaatttcg ttgtcggatt
    16621 cgcggaaaaa aatcatgaac agcacggcga taatggcaat catcaccgcg ccgaaagtcc
    16681 acatcccgga ccagttgaaa gtcagtccgt ttaccggttc ctgataagcg aacatctttt
    16741 ccatcatcac accgccaaga cgatagccga gcaaactgcc gaagccctgg cagcagagcg
    16801 tgatcagccc ctgcgcagcg gtacgcatat gcacgggggc ttttttatcg acatagatgt
    16861 aagcggtaac gtagtaaaaa tcgtaactta cgccgtgaag caaaataccg aggaacagta
    16921 acgcgtaggt gaaatattca tccgcactac cgtaaataaa gaagccatag cagatcgcag
    16981 cggtgaccag accaagcaat aatacctttt tgataccaaa gcgtttagtg aaaaacggca
    17041 atgccagcat aaagaagatt tcagagaact ggccgagcgt catccagccg gtggcgtttt
    17101 tcatgccaac ttcggtcaga taaccgttgg caaagatgta atagaacgcc agtggcatcg
    17161 caaacaggaa tgaacagaag aaaaagacga ggaagttttt atcgcgcagc aggatcagcg
    17221 catccaggcc gagcatgact ttaatgtcca ttttgccggt gctttttggc ggcgtgtcgg
    17281 gcaggaaaaa cgcaaacaca ccgagcagag cagaacttcc ggcggtaatc agcagcggga
    17341 tgttagtcgg tgagatatcg gcatacccca gcatttgcgg caagaaacca catgccagac
    17401 cggaggcaat ccagccgata gtgcccatca cacgaatgcg tgggaaatca cgctccacat
    17461 ccggcacgtt ggcaaaagcg atgctgttag tcagcgcaat ggtcggcata taggttagcg
    17521 agtaggccag cagtaacgga aagaacccgg caaaagtggt ctgttgcgca gcgaaataca
    17581 tcagcacagc accagcgaac atcaataccg ccagcacttt ctgcgccgag aaaaagcggt
    17641 cagtgatgga gccaaccaga attggcgaca ggatcgccgc aatggctgtg caggcatacg
    17701 accagccaat ttctccggca ctaaaaccgc ttttacttaa ccacaaccac aatggcacaa
    17761 accacgcgcc ccagataaac cattcaacaa acatcatgag cgacagcttt gctgttgttt
    17821 tcatctttta attccttcat gacaggtaat gtaaggtacg cgatgacaat accacataat
    17881 aatgcctttt gaataccttt gcggtgaaaa cttgaccgcg ctaacatttt ttctgatgaa
    17941 tcgagccaac agaaaacgct gaaaaaacat ccaaaagatg gaaaaactcg cccgtaacga
    18001 ctaggattat gattgcccgc aaaaggcggg ccaggacaat ccatagccga tatccaatcg
    18061 gaatttacgg gagcatagta atgacagata ttgcgcagtt gcttggcaaa gacgccgaca
    18121 accttttaca gcaccgttgt atgactattc cttctgacca gctttatctc cccggacatg
    18181 actacgtaga ccgcgtgatg attgacaata atcgcccgcc agcggtgtta cgtaatatgc
    18241 agacgttgta caacaccggg cgtctggctg gcacaggata tctttctatt ctgccggttg
    18301 accagggcgt tgagcactct gccggagctt catttgctgc taacccgctc tactttgacc
    18361 cgaaaaacat tgttgaactg gcgatcgaag cgggctgtaa ctgtgtggca tcaacttacg
    18421 gcgtgttggt gtcggtatcg cggcgctatg cgcatcgcat tccatttctc gtcaaactta
    18481 atcacaacga gacgctaagt tacccgaata cctacgatca aacgctgtat gccagcgtgg
    18541 agcaggcctt caacatgggc gcggtggcgg ttggtgcgac tatctatttt ggctcggaag
    18601 agtcacgtcg ccagattgaa gaaatttctg cggcctttga acgcgcgcac gagctgggca
    18661 tggtgacagt gctgtgggcc tatttgcgaa actccgcctt taagaaagat ggcgttgatt
    18721 accatgtttc cgccgacctg actggtcagg cgaaccatct ggcggcaacc ataggtgcag
    18781 atatcgtcaa acaaaaaatg gcggaaaata acggcggcta taaagcgatt aattacggtt
    18841 ataccgacga tcgcgtgtac agcaagttaa ccagcgaaaa cccgattgat ctggtgcgtt
    18901 atcagttagc taactgctat atgggccggg ctgggttgat aaactccggc ggtgctgcag
    18961 gcggtgaaac tgacctcagc gatgcagtgc gtactgcggt tatcaacaaa cgcgcaggcg
    19021 gaatggggct gattcttgga cgtaaggcgt tcaagaaatc gatggctgac ggcgtgaaac
    19081 tgattaatgc cgtgcaggac gtttatctcg atagcaaaat tactattgcc tgatacgcct
    19141 catacgaaca gcaccacccc gtaggacgga taagacgttt acactgcatc cggcaaccta
    19201 tgcctgatgc gacgcggaag cgtcttatca tgcctacata gcactgccac gtatgtttac
    19261 accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg gaggccgttt
    19321 ttttgccttt cactcctcga acaattttca tattgttgtt tttgtgatca ttatctcgac
    19381 atttaaaagc aaataatttc attatatttt gaaatcgaaa gcaaacgaca agatatgaaa
    19441 atgtacgtgg tatcgacaaa gcagatgctg aacaacgcac agcgcggcgg ttatgcggtt
    19501 ccggcattca atattcacaa tctcgaaacg atgcaagtgg tggtggaaac cgctgccaac
    19561 ctgcatgcgc cggtcatcat cgccggaacg cctggcacat ttactcatgc tggtacagaa
    19621 aatctgttgg cgctggtcag cgcgatggcg aagcaatatc accatccact ggcaattcat
    19681 ctcgaccatc acacgaaatt tgacgatatc gctcagaagg ttcgttctgg cgtgcgctca
    19741 gtcatgattg acgcctcgca tttgcctttt gcgcaaaata tttcacgggt caaagaggtg
    19801 gtggattttt gccatcgctt tgatgtcagc gtcgaggcgg agctggggca acttggcggc
    19861 caggaagatg atgtgcaagt caatgaagtc gatgcgttgt acaccaaccc cgcacaggcg
    19921 cgtgaatttg ccgaggcaac cgggattgat tccctggcgg tcgccatcgg cacggctcac
    19981 ggtatgtatg ccagcgcacc ggtgcttgat ttttctcgac tggagaacat tcgccagtgg
    20041 gtgaacttac cgctggtgct gcatggcgcg tcaggattat cgactaagga tattcagcaa
    20101 accatcaaac tggggatatg caaaatcaac gttgcaacgg agctgaaaaa tgccttcttg
    20161 cagtcgttaa aaaattacct gaccgagcac cccgaagcga ccgatccccg ggattatttg
    20221 cagtcggcta aatccgcaat gcgcgatgtg gtgagcaaag tgattgccga ttgtggctgc
    20281 gagggcaggg cataacgcac ctgccattta acaaggaaaa aacatgaaaa cgttaattgc
    20341 ccggcataaa gctggtgaac atatcggcat atgttcagtc tgttctgccc atccgttggt
    20401 tatcgaagcg gcgctggcat ttgatcgcaa cagcacgcgc aaagtgctga ttgaagcaac
    20461 gtcaaaccag gtcaatcaat ttggcggtta taccggaatg acaccggcag actttcgcga
    20521 atttgttttt acgattgccg ataaagttgg gtttgcacgc gaacgcatta ttctcggcgg
    20581 cgatcatctg ggaccaaact gctggcagca agaaaatgcg gatgcggcga tggaaaaatc
    20641 cgtcgagctg gtaaaagcat atgttcgtgc tggcttcagt aaaattcatc ttgatgcgtc
    20701 aatgtcctgc gcgggggata ccataccgtt agcaccagaa acggttgcgg aacgagctgc
    20761 tgtgctttgc tttgctgcgg aaagtgtggc gacagattgc cagcgtgagc aactgagcta
    20821 tgtcattggc actgaagttc cggttccggg cggtgaggcc agcgccattc agtcagtaca
    20881 catcacccat gttgaagatg ccgccaatac tttacgtacg catcaaaagg cctttattgc
    20941 ccgtgggctg acagaggcgt taacacgtgt gattgccatc gtggtgcagc cgggtgtgga
    21001 atttgatcac agcaatatta tccattatca gccgcaggaa gcgcaggcgc tggcgcaatg
    21061 gatagaaaac acccgaatgg tttatgaagc acattctacc gattaccaga cccggacggc
    21121 ttattgggaa ttagtccgcg atcactttgc aatattgaaa gtcggtcccg cattaacctt
    21181 tgctttacgc gaggcgatat ttgcactggc acaaattgag caggaactta tcgcccccga
    21241 aaatcgcagc ggttgcctgg cggtaattga agaagtgatg ctcgacgaac cgcaatactg
    21301 gaaaaaatat tatcgcacgg gttttaacga ttcattactg gatattcgtt acagcctgtc
    21361 ggatcgtatt cgttattact ggccgcatag tcggattaaa aatagcgttg aaacgatgat
    21421 ggtgaatctg caaggcgtgg acatcccact gggcatgatt agtcagtatc ttcccaaaca
    21481 atttgaacgc attcagtccg gggaattatc agcaatgccg catcagctta ttatgaataa
    21541 aatttatgat gttttgcgcg cctatcgcta cggctgtgcg gaataaggac ggtatatgac
    21601 taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt agcgaagttt taacctatat
    21661 cggtaatgag atgctcgcga aaggtgtggt tcatgatacc tggccacagg cattaattgc
    21721 cagagaagca gaattcccta ccgggataat gcttgagcag cacgccattg caataccgca
    21781 ttgtgaggcg attcatgcta agtcgtcagc catttatctg ttaaggccaa caaataaagt
    21841 tcattttcag caagcggatg atgataacga cgtggcggta tcgttggtta ttgcgttgat
    21901 tgtggaaaat ccgcagcagc aattgaaact tttacgctgt ttatttggca agttacaaca
    21961 gcccgatatc gtcgagacac taattactct tcctgaaact cagttaaagg aatacttcac
    22021 aaagtatgtt ttagattcag acgaataaat ccctctgtaa caataaaaag gactatttat
    22081 gaaacgcaag attattgtcg cttgcggagg cgcggttgcg acctctacga tggcggcgga
    22141 agaaattaaa gagttgtgtc agagtcataa tattcctgtt gaattaatcc agtgtcgggt
    22201 taatgaaata gaaacctata tggatggtgt gcatttgata tgtaccactg ccagggtgga
    22261 tcgtagtttt ggcgatattc cgttagttca cggcatgcct tttgtttctg gtgtcggtat
    22321 cgaagcatta caaaataaaa ttctgactat cttacagggg tgacctatgt tttcagaagt
    22381 catgcgttat attctcgacc tcggccctac agtgatgctg ccggttgtca tcattatttt
    22441 ttctaaaata ttaggcatga aggcaggcga ttgctttaaa gcgggtctgc atatcgggat
    22501 tggctttgtt ggcattggcc ttgtgattgg cttaatgctg gattccattg gtccggcggc
    22561 gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg
    22621 ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg gcgattccga ttgcgatatt
    22681 ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg gtaaatgttg atatctggaa
    22741 tatctggcat atgaccttca ccggcgcgtt gctgcacctg gcaaccggtt catggatgat
    22801 agggatggcg ggtgtggtaa ttcacgcggc gtttgtttat aagctctgcg actggtttgc
    22861 ccgcgatacc cgaaatttct ttgagctgga aggtattgct attccgcacg gtacgtcggc
    22921 gtatatgggg ccgattgcgg tgctggtcga tgctatcatc gagaaaatcc caggcgttaa
    22981 ccgaattaaa tttagcgccg acgatattca gcgcaaattt ggcccttttg gcgaacctgt
    23041 caccgtgggt tttgtgatgg ggctgattat cggcatcctc gcgggttacg atgtcaaagg
    23101 tgtattgcag ctggcggtaa aaacggcggc agttatgctg ctgatgccac ggatgattaa
    23161 acccatcatg gatggtttaa cgcccatcgc taagcaggcc cgtagtcgtt tacaggcgaa
    23221 gttcggcggt caggagttcc tgattggcct ggatcccgca ttactgctgg ggcatacggc
    23281 ggtggtatcg gcaagcctga tttttattcc gctcaccatt ttaattgctg tttgtgttcc
    23341 gggtaatcag gtactgccgt ttggcgatct tgccactatc ggcttcttcg tggcgatggc
    23401 ggtcgcggtg catcgtggaa atctgttccg caccttaatc tcgggtgtca tcattatgag
    23461 catcaccctg tggatcgcga cgcaaactat tggtttgcac acccaaccgg cggctaatgc
    23521 tggggcgtta aaagccgggg gtatggtggc ttcaatggat cagggcggtt ctcccattac
    23581 ctggttactg attcaggttt tctccccgca aaatattccc ggtttcatta ttatcggcgc
    23641 aatttatctg accggtattt tcatgacctg gcgtagagcg cgtggtttta ctaaacaaga
    23701 gaaagccgtt ctcgcagaat aatttttacc tgagggggag gttatccccc ttaacaatca
    23761 ggagttttta tgaaatcagt agtgaatgat actgatggta tcgtgcgcgt tgcaaaaagc
    23821 gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa aaattgccag ctcgggctta
    23881 tgtggttccg atttacccag gatatttaaa aatggtgcac attattatcc aataacgtta
    23941 ggccatgaat ttagcggcta tattgatgcg gtgggatccg gtgttgatga tttacaccct
    24001 ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta cttgtccaga gtgtttgaaa
    24061 gggttttatt cccagtgcgc aaaatatgat tttattggct cgcggcgtga tggtggattt
    24121 gctgaatata ttgtcgttaa gcgaaaaaat gtctttgctc tacccacgga tatgcctatt
    24181 gaggatgggg cttttattga gccgattacc gttggcctgc atgcttttca tttagcgcaa
    24241 ggttgtgaga ataaaaacgt tattattatt ggtgccggaa ccattggcct gctggctatt
    24301 cagtgcgctg tcgcgctggg agcaaagagt gtgacggcga tcgacattag ctcagaaaaa
    24361 ctggcactgg caaaatcttt cggtgcgatg caaacattta acagtagcga aatgagcgcg
    24421 ccgcaaatgc agagcgtttt acgcgaactg cgctttaatc agcttatcct cgagacggct
    24481 ggcgtaccgc aaactgtcga actggcggta gagattgccg gtcctcatgc ccaactggcg
    24541 ctggtgggca cgttgcatca ggatctgcat ttaacatcgg caacgtttgg caaaatattg
    24601 cgtaaagagc tgacggttat cggcagttgg atgaactatt ccagcccttg gccggggcag
    24661 gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt taagcctgga gccattaatc
    24721 gctcaccgtg gaagctttga aagcttcacc caggcggtgc gtgacatcgc tcgtaatgcc
    24781 atgccgggca aagtgttgct cattccatga aaccgcgggc cagcgtgatg ctggcccggt
    24841 attgtgcaaa acagatcatt caccaatggt cccccttcgt ttacactagc cacaattgaa
    24901 tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata agatcatcca attagtgaat
    24961 gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat ttgctgcctc ggaagcgaca
    25021 atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg ttacgcgctt tcatggcggt
    25081 gcggcgaaaa taatgtccgg taatagtgaa accgagaccc aggaagtcgg gtttaaagag
    25141 cgatttcagc tcgccagcgc gccaaaaaac agaatagcgc aggcggcagt caaaatgatc
    25201 catgaaggga tgaccgttat tctcgacagc ggaagtacca caatgcttat cgccgaagga
    25261 ttaatgaccg ctaaaaatat cacggtgatt accaacagtc tcccggcagc gtttgccctt
    25321 tccgaaaata aagacattac tctggtcgtc tgtggtggca cggtccgcca taaaacgcgc
    25381 tcgatgcatg gttccattgc cgagcgttca ttgcaggata ttaatgccga tttaatgttt
    25441 gtcggtgctg atggtattga tgcggttaat ggtattacga cctttaatga aggttattcg
    25501 attagcgggg cgatggtcac cgccgcaaat aaagtgattg ccgttctcga ttcatcgaaa
    25561 tttaatcgtc gcggttttaa tcaggtactg ccaatcgaaa aaattgacat tattgttacc
    25621 gatgatgctg tgtcagaggt ggataaattg gcattgcaga agacacgggt aaaattaatt
    25681 acggtgtagt attgttctgt atgccggata aggtgtttac accgcatccg gcattacgga
    25741 ttctccctgg ttcaagagag aatagtttga ttaacgcaat aatggacaat ctggtggtaa
    25801 tcgacaacgc aacgccgccg gaagtatttc aatatggaag ttttgcccac tcaacggttc
    25861 gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata tcaaaccacg acgaagcgcc
    25921 ttcgataata ttcgggttat cttcgtcaga ttttaaggtt gatacgagag ccggaagaat
    25981 ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg ttaattaacg cgttcgggca
    26041 caattgttga ccgccaccgg cctgacgccc gttaccaata ccaatgacca gggcgtcacc
    26101 ttgccagtga aagttttcac cgcggatttc acaacggtcc ggttgcagag tatccatgcg
    26161 cattaagcca tgaatgatgt aagagacgcc acccagcgcg gcttttaatt tttccggtgt
    26221 ttctgtggta atacgcgtcc caaatccgcc tgtcgccata ttaataaaac aggtttgttt
    26281 gttgacctgc gccatatcta tcgcaatggc attaccggca attgccagtt tcagcgcctt
    26341 atccagtgcc tcaggaatcc ctacactggt ggcaaaatca ttggcagttc ctaatggcaa
    26401 aattcccagc gcgggtatgt catccccctc acactgaatc aacgccgtag aaacttcatt
    26461 aatggtgcca tcgccaccac cggcaatcac cgttgcgacg cccaacttcc gggcctcctc
    26521 tacaaatcgt gcggcatcac ctttctccca ggtgacccgc acatggatcg tcattccttc
    26581 ctcacgcaac agcataattg cttcgcgcaa gggtagattg tcagtacttt taccattaag
    26641 aatcagtaag ctggcgggaa attctgccat gatcgtatgt gcctttatga ttggtctgta
    26701 gatagtgtag agcagaaaac aaaaggtgga gtcagaacag gatgaaagtc ggaggataaa
    26761 aagtcagttc aagtatggag gattcagtgg ttaccaccag gagcttgcaa tggggacagc
    26821 gaatactaac ggctgccacc gataaatttc aaaaaagagc atatacctaa tattcaacta
    26881 aacagtggca tcttcaatat aatatattaa agcccccatg gagttaccct gaagggcctc
    26941 aatgtccgta attcctactt atgtaggaaa tgttgtacag aacatttatt ataatcctat
    27001 tcaattataa taatcatgcc attattatat ttaaacacta gagagtgtcg ttggtattta
    27061 atgggggaag gtgagatgaa aaagatagct gctatatcat taattagtat ttttcttata
    27121 tctggttgtg ctgtgcataa tgatgaaaca agtatcggta aatttggtct tgcatataaa
    27181 agtaatattc agcgtaaact cgataaccaa tactacaccg aagccgaagc ttctttagcc
    27241 aggggcagaa tatctggtgc agaaaatata gtaaaaaatg atgcagccca tttctgtgtt
    27301 actcagggca aaaaaatgca gatagttgac ctgaagacag aaggtgcagg attacatggc
    27361 gtcgctcgtc tgacattcaa atgtggagag tgagaatatt tttttgtaag cgtcaaacat
    27421 gcgcgttctg gctgtgcgta gccggaacct gggcgagcac gataccgtta cgtgaaaggc
    27481 attgtgctat gaagggggat tctatcgatg tggtcaatgg aagacggtta tcagggatca
    27541 atccttcttt acagcttatg tacaaaaaat aagcccgtgt aagggagatt acacaggcta
    27601 aggaggtggt tcctggtaca gctagcattt tatggattat gtttttcagc gaaacggatg
    27661 ataaccttaa taaatgcagc tgtatgtgat cggtttctaa gaattttcca tccgggaaaa
    27721 ataatcgaaa ttaatcactt accgtggggg ttacgcgtgg tttccccgga gaaattacgc
    27781 attagcagcg cgtaatttag ctcaagatcc tgcgggaccg ggagccacac agtataacca
    27841 tcgcctggtg cgaccggcat agcttcgcct ttggcgtttt ccatgtgctc aagggtaaag
    27901 ttaatgttgc cttgcggcgt catcagctca agactgtcgc caacggagaa tttatttttc
    27961 accgctaccg ctgcgaggtc ccccttgcgc tcaccggtaa actcaccaac aaactgctgg
    28021 cggtcagaaa ctgaataacc gtattcgtag ttctgataat cgtcgtgagt atgacgacgc
    28081 aggaaacctt cggtatagcc acgatgcgcc agaccttcca gagtttccag caggctggta
    28141 tcgaacggtt ttcccgcagc ggcgtcatcg atagctttgc ggtaaacctg tgcggtgcgt
    28201 gcgcaatagt agaaagattt ggtacgacct tcgattttca gcgaatgcac gcccattttg
    28261 gtcaggcgtt ctacatgggc gatggcgcgc agatctttcg agttcatgat gtaagtgccg
    28321 tgctcatctt caaacgcggt catatactcg cccggacgct gggcttcttc gatcataaac
    28381 actttgtcgg ttggcgcgcc gatacccagc gtcggctcaa cattttgcac cggaatcggc
    28441 tcgtacttgt gtacgatgtt gccaacgtca tcttctttcc cttcctggac gttgtactcc
    28501 cagcggcagg cgttggtgca ggtgccctgg ttcgggtcgc gcttgttgat atagccagag
    28561 agcaggcagc gaccggagta ggccatgcac agtgcgccgt gaacgaagat ctcgatctcc
    28621 atatccggca cctgattatg gatctcttca atctcttcca gcgacagctc gcgagagagg
    28681 atcacgcggg tcagtcccat ttgctgccag aatttcaccg tcgcccagtt cacggcgtta
    28741 gcctgcaccg agaggtggat cggcatttca gggaagtgct cacgcaccag cataatcagc
    28801 cctggatcgg acataatcag cgcatccggc cccatttcca ccaccggttt caggtcacgg
    28861 ataaaggttt tcagcttggc gttgtgcggt gcaatgttga ccacgacata aaactttttc
    28921 cccagcgcgt gggcttcatt gatgccgagc tgaagatttt cgtggttgaa ttcgttgttg
    28981 cgcacacgta gggagtaacg cggctggccc gcataaacag catctgcgcc ataagcgaaa
    29041 gcgtaacgca tatttttcag cgttcccgcc ggggaaagga gttccggttt aaacataatt
    29101 ctctcgttct gatgacaggt cagatccgct tcacctgatg aagcggttag gggaggcccc
    29161 cactttaagg gcgggcattg tagcgctgta ggggggatct ggtaaagcgc cggacgccaa
    29221 caacccacgc ccggcatcgt aattacgtat tgtctttcac cgtctggctt gtgccattgg
    29281 ctttaacgga ggcaattccg gtttcacgag attgcgcggt ggcgtacatc tggctggagc
    29341 cgataatttg atgattagcg gctttcagat tgaaatagaa tttgccgttg cttgccgttt
    29401 tttctcatag cgctcttctt gcgggctgtt gctacgcacc gacgcgatgc ccttttccgc
    29461 agaggctttt gaggtataca gctcgctggt gaggatagtc tcaccattgc ccgcttttag
    29521 cacaaaccgg aactgattat cactgctctt actgagttca aaccaaccag ccataatgac
    29581 tccttgattg aaattgctta tcgaaaacag catctaaagg tgctgtttac tgattaaagt
    29641 atgagcgatt ttctgatgcc tgccagggcg gagagataat gtcagtttgc tgaccatgcg
    29701 tggaataatg ttgccggatc gggtctgaac gccatctccg gctaacaaag taatgtcgct
    29761 aaaactaaac gatgcggcag gcgtcggctt cccagcggta accaacgccg taaacggcgc
    29821 ggataaatga ctgttcggcg tcgagagatt ccagcttgcg gcgcagattt ttaatgtggc
    29881 tgtcaatggt gcggtcggtt actacgcggt agtcgtcata aagatgattg agcaattgct
    29941 cgcgggagaa cacttttcct ggttcgtgag agagcgtttt aagcagacga aattccgcag
    30001 gcgtcaggtc aagcatttta ccgcgccatg aagcctgaaa acgaccttcg tcgataatca
    30061 acgggctttc ggcatcctgt tgctgaagtt cgcgttgcgg tttacaacgg cgcaaaatgg
    30121 ttttgacacg cgctaccact tcccgtgggc tgtacggctt gcagatgtag tcatcggcgc
    30181 caatctccag ccccagcagg cggtcgatct cttcgatttt tgccgtcacc atcacgatcg
    30241 gaacatcgga aaaacgacga atttcccggc ataatgtcag gccatcggtg ccagggagca
    30301 tcagatccaa caggatcaga tccggtggtg tctggcgcac atacgccagt acctgatcgc
    30361 cgtggctgat aagcgtcggc gcatagctcg cagcacgcag ataatcaatg agcaactgcc
    30421 ccagcttcgg ttcatcttcc acgatcaaaa tacgcggtgt gttttcgtcg attggtaact
    30481 cggtcatact tctctctgta aatcccgttc cagcggtaac tctactgtaa tgcttacccc
    30541 gccaaaaggc gaatgggcgg caataatgcg gccattatgg gcttcaacaa tgttcaggca
    30601 aatcgccagc cccagcccgg aaccgccgct ggcgcggttg cgggaacctt cggtgcgata
    30661 aaaacgttca aacaattttt gtagctgatc gtcactgaca cctggcgcac tgtcggcaaa
    30721 ggtcaggcgc accgttttgt cgtgctgctc ggcagagatt ttcaggctgc cgccgctgtc
    30781 agtgtagcgc aggctgtttt ccagtaagtt attgaataac tgcattaaac ggtcgcgatc
    30841 gccaaatacg gtgatactgt ctggcaggga aaattgcagt ttcaggccac ggctggcgaa
    30901 tcgttcgcga aatgcgccgc ccgccacttc cagcagtggg atcaaatcta ccggtgcttt
    30961 ttgataggcg agagcgcctt catcagacat cgacaactga tgaagatcgt caaccagttt
    31021 ggtcagtgta ccgacctccg cctgtaaaga cgccaccgtc tccggcgtga atttgcgcac
    31081 gccatcctga atggcttcca gttcaccgcg cagcaccgct aatggcgtac gcagttcgtg
    31141 agaaatatcg gccataaaat cgcgccgcat ttgctggttt ttctccagcg tgctggcgag
    31201 ctggttgaag tcttgcgcca gtttgcccag ttcatcttca ctggtgggcg ttacgcgggt
    31261 agtgaaatcg cccgccgcca gtttgtgcgt gccatcgaca agtcgtttta ccggtgccag
    31321 caaaccgcgc gccagcagaa aggtggcgag cgccgcgagt aacgttgcca gggcgacgat
    31381 cagccagctg gtttgccgct gttgtttatc gaaattgata tcagtattgc gggtcagccg
    31441 ctcaacaggg gaggcaatca ccgcgcccac ttccgcaccg ttgaccagaa tgggtcgccg
    31501 tgtaccgtca ggtggaatcg gcgctcgcgg accaaccagt actttgttgt tttgatcgac
    31561 cacccagaac tgggtacgcc agccgtgcgg cggcataccc gggccgggtt tatcttccga
    31621 attatcgtgt tcaaatgaac gcaggatctg aaagacaaag cgatcgttgt tgcgcaggaa
    31681 gcgccagtta ccatgctgcg catactgctc gccaagcgca tcacttaaca gctgtaatcg
    31741 ctgttcattg ccgtgtttga tgtaatcaat aaagccacgc tcaaaactga tacgcaccgc
    31801 ccagtgcata ctgatcagca agacaatgca ggtggcgaaa atcgccagaa acagtttgcc
    31861 ggtaataccg ggtcgccaga acttcattgc gcgctccttt ttcgccgcga aatagctaca
    31921 ttttgatgcg tatcgttcgg cactctggca aagatgaacg ccggaagggc gatgataaac
    31981 gccatgctaa gccaggtgta cataaagacg gtttgtgtgg tgccgctgtc gacgctgata
    32041 tgctgcgaac caaaaagccc cagcaacagc ccggcgatgg tgacgccgat actcatcgac
    32101 aattgcataa tcatcgacag caggctgttt ccgctgctcg ccagattgtc cgggagatct
    32161 ttcagcgtca gggtgttcat ggaggagaaa cgggtcgagt tgaccatccc ttgtaaaaac
    32221 aggacgaacg gcaaaacgta gtaccagccc aacagagcgg tagtcataaa caacagggtg
    32281 accagcgaca gacccagcgt ggtcgctacc agtacccgac gataaccaaa gcgattcacc
    32341 acctgtacca caattcgctt cattcccatg ctgccaagca ccatcgggat catcatcagt
    32401 ccggcatgaa acggcgagaa accgaggcca atttgcagga aaaccggtgt cataaagggc
    32461 aacatgccac tgccaatacg tccggcaaag ctccccgcca ggcccagcga aaaggtacga
    32521 gtgcggaaca gtttcagact gaacagggcg cggttgttat ttctggcgtg cagcagataa
    32581 agcaccagtg ccaccacgcc aactgcgacc aggccagcaa tcgccagcgg cgataaacct
    32641 gtacctttac tgccgtccag cgccagggtt aataccgcca tgccaaccgc cagcaataaa
    32701 aagccggaaa gatcaaagcg ccgcgtctgc atggtgtagt tcggcattaa catcaatgtg
    32761 gcgatcgcac cgataatccc caccggaatg ttgatcaaaa agatccagtg ccacgacgcg
    32821 tattctacca gcagaccgcc gagcgccgga ccgagcagcg gaccgacctg accgggtaac
    32881 gtgacaaagg tcatcgccgc catatattgc tcgcgcggta cgattttcat caccgtcaac
    32941 ctgccgaccg gcaccatcat cgcaccgcca acgccctgta acgcgcgtgc cagcaacagt
    33001 tcgttcagcg tgccggaaag cgcgcaaaac agtgaaccga gagtaaacag cacgatggcg
    33061 gtaaagaaaa tattgcgcac gccgactttg tccgccagcc agccgctggc gggcagcatc
    33121 accgccacgg tcagcacata agagacaatg accatgtgca tatgcaacgg actttccccg
    33181 aggctttgcg ccattgaggg aagggcggtg tttacgatgg tggtgtccag cgactgcata
    33241 aagaagccga aagccacaat ccataattgc cagcgggtgc tgtcgggaag atctgtcatt
    33301 tactcggtta ccgtttgttt aggtttacgc gaaaaacgca gccgcagacg gtcgaaaaag
    33361 agatacacca ccggcgtggt atacagcgtc aggagctggc tcatcaccag accgccgaca
    33421 atggtgatct ccaggggttg ccgcagctcc gagccgtcac cgcccgacaa taccagcggc
    33481 agcgcgccaa acagcgccgc cagggtggtc atcataatcg ggcgaaaacg cagcagacag
    33541 gcctggaaaa tagcttcctg cggcgtcagg ttaccgtgcc gttgggcttc aagcgcaaaa
    33601 tcgaccatca taatggcgtt tttcttcacg atgccgatta atagcatgat ccctatcagg
    33661 gcgattaggc tgaacggggc attgaacagc tccagcgcca acagcgctcc aacgcccgcc
    33721 gagggcaggg tggagagaat cgtcagcgga tgtacgtaac tctcgtaaag gatacccagc
    33781 acgatataca ccgtggcgat ggcggcgata atcaggatca cctgcgagtt catcgtctcc
    33841 tggaaaacct gcgccgtgcc ggcaaaactg ccgcgcaccg tcgaaggcac accaagctgg
    33901 gtcattgcgc gatcgatcgc cgcactggcg tccgagagcg attttccggt cggcaggttg
    33961 aacgagatgg tcgaggccgc cgataatccc tgatgattca ccgatagtgg ggcattcgcc
    34021 ggttgccatt tagcgaaata cgacagcggg atcgctttgc cttcgttatt gataacgaac
    34081 attttttcca gcgcactgat gtcctgggta tagcgcggat ccacttccat caccacttta
    34141 tactggttca tcggctggta aatggtcgag atttgccgct gaccgaaggc gttatttaac
    34201 agactgttgg cggcctgtac gtcgattccc agccgtgcca tggtgtcgcg gtcgtaaacc
    34261 agattcatct ccgcgccgtt atcctgctga tcggagttca cgtccgccag ttccggcaac
    34321 gtcgccagtt ttttgcggat tttcggctcc cattcgcgca gtgccgccag gtcgtcggat
    34381 aacaacgtgt actggtagct ggcgttcgac tgacgcccac caacgcgaat atcttgtacc
    34441 gccatcagga acagattcgc ccccggttct ttcgccagct tcacgcgcag acggtcgata
    34501 atttgctgcg ccgtttcgct gcgttcgtcg cgtggcttga gggtgataaa catcatccca
    34561 ctgttaactc gtgaaccgcc ggtaaagccg gtgacattat ccactgccgg atcgtcacgg
    34621 ataattttca tgaaatcctg caatttgccg cgcatcgcct gaaacgaaat actctgatcc
    34681 gcctgtatcc cacccatcag tacgccggtg tcctgctccg ggaagaaggt tttcgggatc
    34741 gagatattca gcgcaatggt accaagcagc accacgccca ccagacgggt atgattgagc
    34801 acccatttca gcgacttgcc gtagccttgt tgcagggcta ccaacatgcg gccaaaacca
    34861 cgcagtcgct tttgctcacg cggcttgctg gcttttagca tccagccgca catcattggc
    34921 gttagcgtca gggaaaccag caacgaaata ccaatggcga cagaaagcgt cacggcaaac
    34981 tcgcgtaaca gtcggcccgg caatccgccc atcaacagca gcggcaggaa caccgccacc
    35041 agtgacagac tcatcgacag caccgtaaag ccgacttcgc gagtaccttg cagcgcggcc
    35101 tgtagtggtt tcatccccgc ttccagatga cgggcaatgt tttccagcac cacaatggcg
    35161 tcatccacca cgaaaccagt agcaatggtg agcgccatca gcgaaagatt attgagactg
    35221 aatccgcaca ggtacatcgc cgcaaacgta ccaatcagcg aaaccggcac cgcaacggcg
    35281 ggaataatag tggcgcgacc cgagcgcagg aataaaaaga ccaccagaat caccagcgcc
    35341 accgagataa tcagcgtttg ctcgacttct tccagcgagg cgcgaatggt gggggagcga
    35401 tcctgggcaa tttgcagatc aatcgccgcc ggaatggtct cctgcaactc tggtaatttt
    35461 gcccggatgc tgtctaccgt ctggataata ttggcttccg gcagtttgcg gatcatcagt
    35521 aaaatagccg gtttggcgtt ggtcatcccg gcgttgcgca catcctgcac tgagtcagtc
    35581 accgtcgcca catcgcccag acgaaccgcg ccgccgttgt tgtagtgaat aatcagcggc
    35641 tgatattcag cggcggtttt cagttcatcg ttggtctgga tctgccagcg gtgagtgcca
    35701 tcttccagcg ccccctgcgg tttacgcacg ttggcattgc tgatggcagt gcgtacgtcg
    35761 tccagcgaca cgccctgatt aaacagcgcc tgcggggtca gcccgacgcg cacggcgggc
    35821 agcgagctgc caccgacatc gacatcaccg acaccgtcga tttgcgaaat cgtcggagcc
    35881 agctgcgtcg aggcgaaatc gtacagttca ccctgcgaat aagtatcgga tgtcagcgtg
    35941 aggatcataa ttggcgcatc cgacgggttc gctttgcgat aggtcgggcg gctgggcatc
    36001 ccgctgggca gcaaactttg tgcagcgttg atcgccgcct gcacatcacg cgctgcgccg
    36061 ttgatatccc ggtcaaaatc aaactgcaaa ataatgcgcg tgctgccgag cgaactgctg
    36121 gaggtcattt cactaacccc ggcaatgcgc ccaagtgagc gctccagcgg cgtggcaacg
    36181 gaagacgcca ttgtctccgg cgacgcaccg ggcagcgagg cgctgaccat aatcaccgga
    36241 aaatcgacct gcggcagcgg ggcgaccggc agcatacgga aacccagtat gccgcacagg
    36301 gtaatggcga ccgacagtaa aatcgtcgcc accgggcggt aaatgaagag ggcaaaaaac
    36361 ttcacttacg cctcctcttc atggcgagca aagcgacttt tggtccacaa tgccaggcgg
    36421 tcgaacagca gataaatcac cggcgtggtg aacagcgtca gcacctggct gacaatcaga
    36481 ccgccgacca tgccgatacc taacggacga cgcagttccg cgccgacccc ggtactcaac
    36541 atcagcggca gcgcgccaag cagagccgcc agggtggtca tcaggatcgg acgaaaacgc
    36601 aacagacaag cctggtagat tgcatcgcgc ggcgacatgc cttgctcgcg ctcagcagcc
    36661 agcgcgaagt cgatcatcat gatggcgttc ttcttcacaa taccgatcag caaaataatg
    36721 ccgataatcg caatcacatc cagttcgcta ccggcaatca ttaatgccag cagcgcgccg
    36781 acccctgcgg tgggtagcgt cgagagaatg gtgatcgggt gaataaagct ctcgtacaga
    36841 atgccgagca cgatatacat cgccaccacc gccgcgacaa tcagccagac agtgctgccc
    36901 agcgccgact ggaaggcgag ggtgctgccc tagaattgcg tggtgatatc caccggcaga
    36961 ttcagcgtct tttcggtgtc cataatcgcc tgcaccgcat cgcccagcga atagttatcc
    37021 ggcacgttaa aggagatggt tgttaccggg aactgatcca gatggttgat ggaaagcggc
    37081 gcaaaacgct gctcaatttt ggcaattgag cttaacggca ccacgccacc gtcgctgctg
    37141 gtcaggcgaa tggtatccag cgccgcgagg cctggggtat tttcggtatt gtgctccagc
    37201 accacgcgat actggttagc ctgagtataa atagtggaaa tcagtcgctg accaaacgcg
    37261 ttgtacaggg cgttatcgac gtccgccatg ctgatcccca gacggctggc gctgtcgcga
    37321 tcaacattga catacgccac cagccctttg tcctgccagt cgctggagac atcagaaagc
    37381 tgtggcagtt gctggagttt ttccatcaac tgtggcaccc aggtactgag cgcatccagt
    37441 gacgtggcct gcaaggtaaa ctggtactgg gtgcggctga cctgagtatc gatggtcagg
    37501 tcctgcgttg gttgcaggaa gagatcgacg cccggcactt tatctaccgc cgtttgcaga
    37561 cgggcgatga ctttttgcac ccgatcatca cgttcatcca acggtttgag gttgatttgt
    37621 aaacgtgcac tattcagcga cgggttagtg ccatcaacgc caacaaatga ggtcaggctt
    37681 tgcactgccg gatcctgcaa aatcacgtcc gcgacctggc gttgtcgctg ggccatattg
    37741 gcaaaggagc tggattgcgg tgcctgcaaa gtgccctgaa taatgccgtt gtcctgtacc
    37801 gggaagaaac ctttcggaat gaacacccac agcagcacgc taagcagcag cgtgctgagt
    37861 gccacgctta aggtcagcca cggatgattc agcactttcg ccagtccacg accataggcg
    37921 gcgattatcc tgtcgaacat tttttccgag gcacgggaga agcggttctg tttacgcaac
    37981 gactcctggc tgagcatccg cgcgcacatc atcggtgtca gggtcagcga caccaccgct
    38041 gagatcaaaa tcgctaccgc cagggtaata gcaaattcgc ggaacagtcg cccgacgata
    38101 tcgcccataa acagcagtgg gatcaacacc gcaatcagtg agaaggtcag cgagataatg
    38161 gtaaagccga tttcacctgc gcccttgagc gccgccgcca acggtttttc gcctttttcg
    38221 atatagcggg aaatgttttc gatcaccacg atggcgtcat cgaccacgaa tccggtggcg
    38281 atagttaacg ccatcagtgt caggttattg attgaaaaat cgagaaacac cataaccgcg
    38341 aaagtgccga ttaacgacag cggtacagca acaccgggaa tgatggtcgc cggaatattg
    38401 cgcaaaaaca ggtagataat catgactacc agcgcgatag ccatcatcaa ttcaaactga
    38461 gtatcatcga cggatgcgcg gatattggtg gtgcgatcgg aaagcactgt caccttcacc
    38521 gatttcggca gactctcagt gagctgtggc agcatctgcc gaatgctgtc ggcggtggag
    38581 ataatgttag caccgggctg gcgctgaaca ttcatcacaa tggcctgttc tttgttcgcc
    38641 cacgcgccga gccagctgtt ttctgcacct tgctctacag ttgcgacatc gcccagacga
    38701 attggcgcgc cgttctggta ggcgatgatt agctggcgat actcttcggc ggattgcatc
    38761 tggtcgttcg cggaaagcgt gaccgcacgg gaagggccgt cgaggctacc ttttgccgag
    38821 ttaacgttag cgccggtaat ggcggtgcgc acggtttcgc tggtcaggcc gagggcggca
    38881 atcgcctgag cgttaagttt gacgcgaaca gccggacgct gaccgccgga aagcgtcacc
    38941 aggccgacgc cggaaatctg cgagattttc tgcgcgacgc gggtttccac catatcttcc
    39001 acttgcgtca tcggcatggc ggttgaggtg acggcgagcg tcatgatcgg cggatctgcc
    39061 gggttcactt tgctgtaaac cggcgggtta ggcagatcgc tcggcaacaa gttggtcgca
    39121 gcgttaatcg cggcctgcac ttcctgctcg gcgacattga gcggtaatgt tagctggaac
    39181 tgcaaagtga taactgacgc accgccggaa ctttgcgacg acatctgttt caggccagac
    39241 atctgcccga actggcgttc gagcggcgcg gtaacggcag aggtcatgac atccgggctg
    39301 gcacctgggt agagcgtgac cacctgaatg gtcggatagt ccacttccgg cagcgccgaa
    39361 acgggcaggg cgcgataacc gataatcccg gcgagtaaga tcgccaccat cagcagcgtg
    39421 gtggcgacag gacgcataat aaacaggcgc gacgggccgc ctgtgctgct cgggggtaac
    39481 acctgcatca ggagcgtgct ccttttttcg cgtattcgcg gctggtggct ttctcttccg
    39541 gagtggtggc gctctgggct tccaccactt ccactttcgc cccttcggtc aggcgatcaa
    39601 tgccgtctgt caccacgcga tcgcccgcag aaatacctgc acggatcacc actttctgac
    39661 tgtcctgaat gcccggcgtc accagatgtt tgctgacctt gttttcgcta ttcagcaccc
    39721 agacaaaatg gccttcattg cccatttgca gggcggctgt tgggatcact acggcgtttt
    39781 gttcggtgtc gactaacatg cgcgcgttaa caaactgatt gggaaacagc gcatcatcct
    39841 gattattaaa gcgtgctttc actttaatcg taccggttgt ggcatcgatt tggttatcga
    39901 ggcttaacag cgtgccttca cttaatttct tcgaattggt gcgatcccag gcttctacca
    39961 tcagcggttt tccggctttt tgcgcctgca ctacagtagc gatatcgctt tccggcaggg
    40021 taaagactaa atcgatagga tgcgtctggg tgatcaccac aattccggtg gtatcaccac
    40081 tggagatttg gttaccaaca tcaacttgct tgagaccaac gcgaccatcg actggtgcgg
    40141 tgatgcggct ccagtcgagt tgcaactgcg cgctggcgac gcttgcttca tcagccttaa
    40201 tggtgccttc ggtttcactg accagcgcct gttgggcatc cagctcctga cgggagacga
    40261 gattggtttt tgccagttgc tgataacgcg ccagatcacg gcgggcgtta gcaagcgtgg
    40321 ctttatcttt tgccagttgg ccctgggttt gtgctaatgc aactttgaac tggctggggt
    40381 caatttctgc cagtaaatcg cctgctttga cctgctggcc ttcctggaaa tgtaacgcca
    40441 tcagttgacc gtccacgcgg ctgcgcaccg taacagtatt agcggcggta atggtgccaa
    40501 gcccggtgag gtaa
//
LOCUS       DAJTRB010000028        39746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTRB010000028 DAJTRB010000000
VERSION     DAJTRB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.028"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..157
                     /locus_tag="OO330_001578"
     CDS             <1..157
                     /locus_tag="OO330_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5882488.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            386..976
                     /gene="uidR"
                     /locus_tag="OO330_001579"
     CDS             386..976
                     /gene="uidR"
                     /locus_tag="OO330_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5882489.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1364..3067
                     /gene="uidA"
                     /locus_tag="OO330_001580"
     CDS             1364..3067
                     /gene="uidA"
                     /locus_tag="OO330_001580"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5882490.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3073..4098
                     /gene="uidB"
                     /locus_tag="OO330_001581"
                     /pseudo
     CDS             3073..4098
                     /gene="uidB"
                     /locus_tag="OO330_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4137..5402
                     /gene="uidC"
                     /locus_tag="OO330_001582"
     CDS             4137..5402
                     /gene="uidC"
                     /locus_tag="OO330_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5882491.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5628..7136)
                     /locus_tag="OO330_001583"
     CDS             complement(5628..7136)
                     /locus_tag="OO330_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5882492.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7237..8412)
                     /gene="manA"
                     /locus_tag="OO330_001584"
     CDS             complement(7237..8412)
                     /gene="manA"
                     /locus_tag="OO330_001584"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5882493.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8611..10257
                     /gene="fumB"
                     /locus_tag="OO330_001585"
     CDS             8611..10257
                     /gene="fumB"
                     /locus_tag="OO330_001585"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5882494.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10400..11803
                     /gene="fumC"
                     /locus_tag="OO330_001586"
     CDS             10400..11803
                     /gene="fumC"
                     /locus_tag="OO330_001586"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5882495.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11800..12729)
                     /gene="tus"
                     /locus_tag="OO330_001587"
     CDS             complement(11800..12729)
                     /gene="tus"
                     /locus_tag="OO330_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5882496.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="OO330_001588"
     CDS             complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="OO330_001588"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5882497.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVSPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14285..15604)
                     /locus_tag="OO330_001589"
     CDS             complement(14285..15604)
                     /locus_tag="OO330_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5882498.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15829..16572)
                     /gene="rstA"
                     /locus_tag="OO330_001590"
     CDS             complement(15829..16572)
                     /gene="rstA"
                     /locus_tag="OO330_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5882499.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16701..17036
                     /locus_tag="OO330_001591"
     CDS             16701..17036
                     /locus_tag="OO330_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5882500.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPTAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17033..17755)
                     /gene="folM"
                     /locus_tag="OO330_001592"
     CDS             complement(17033..17755)
                     /gene="folM"
                     /locus_tag="OO330_001592"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5882501.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17792..19174)
                     /locus_tag="OO330_001593"
     CDS             complement(17792..19174)
                     /locus_tag="OO330_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5882502.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19360..20304)
                     /locus_tag="OO330_001594"
     CDS             complement(19360..20304)
                     /locus_tag="OO330_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5882503.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20828..22360
                     /gene="pntA"
                     /locus_tag="OO330_001595"
     CDS             20828..22360
                     /gene="pntA"
                     /locus_tag="OO330_001595"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5882504.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22371..23759
                     /gene="pntB"
                     /locus_tag="OO330_001596"
     CDS             22371..23759
                     /gene="pntB"
                     /locus_tag="OO330_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5882505.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23784..24818)
                     /gene="tqsA"
                     /locus_tag="OO330_001597"
     CDS             complement(23784..24818)
                     /gene="tqsA"
                     /locus_tag="OO330_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5882506.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25229..25594
                     /gene="mdtJ"
                     /locus_tag="OO330_001598"
     CDS             25229..25594
                     /gene="mdtJ"
                     /locus_tag="OO330_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5882507.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25581..25910
                     /gene="mdtI"
                     /locus_tag="OO330_001599"
     CDS             25581..25910
                     /gene="mdtI"
                     /locus_tag="OO330_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5882508.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25949..26770)
                     /locus_tag="OO330_001600"
     CDS             complement(25949..26770)
                     /locus_tag="OO330_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5882509.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26870..26953)
                     /locus_tag="OO330_001601"
     CDS             complement(26870..26953)
                     /locus_tag="OO330_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882510.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27046..27381)
                     /locus_tag="OO330_001602"
     CDS             complement(27046..27381)
                     /locus_tag="OO330_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5882511.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27778..29031)
                     /locus_tag="OO330_001603"
                     /pseudo
     CDS             complement(27778..29031)
                     /locus_tag="OO330_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29138..30040
                     /locus_tag="OO330_001604"
     CDS             29138..30040
                     /locus_tag="OO330_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5882512.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30175..31395
                     /gene="mlc"
                     /locus_tag="OO330_001605"
                     /gene_synonym="dgsA"
     CDS             30175..31395
                     /gene="mlc"
                     /locus_tag="OO330_001605"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5882513.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31520..32215
                     /gene="bioD"
                     /locus_tag="OO330_001606"
     CDS             31520..32215
                     /gene="bioD"
                     /locus_tag="OO330_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5882514.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32168..33460)
                     /gene="clcB"
                     /locus_tag="OO330_001607"
     CDS             complement(32168..33460)
                     /gene="clcB"
                     /locus_tag="OO330_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5882515.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33619..34233)
                     /gene="dmsD"
                     /locus_tag="OO330_001608"
     CDS             complement(33619..34233)
                     /gene="dmsD"
                     /locus_tag="OO330_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5882516.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34276..35130)
                     /locus_tag="OO330_001609"
     CDS             complement(34276..35130)
                     /locus_tag="OO330_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5882517.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35132..35749)
                     /gene="dmsB"
                     /locus_tag="OO330_001610"
     CDS             complement(35132..35749)
                     /gene="dmsB"
                     /locus_tag="OO330_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5882518.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35760..37798)
                     /locus_tag="OO330_001611"
                     /pseudo
     CDS             complement(35760..37798)
                     /locus_tag="OO330_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37859..38968)
                     /locus_tag="OO330_001612"
     CDS             complement(37859..38968)
                     /locus_tag="OO330_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5882519.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38951..>39746
                     /locus_tag="OO330_001613"
     CDS             38951..>39746
                     /locus_tag="OO330_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5882520.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSAS"
BASE COUNT        10118 a         9635 c        10173 g         9820 t
ORIGIN      
        1 aattgaacaa aaaatgttac agcacacgcc gatcagacgt ctgggccaac cgcaagatat
       61 tgctaacgcg gcgctgttcc tttgctcgcc tgcagccagc tgggtaagcg gacaaattct
      121 caccgtctcc ggtggtgggg tacaggagct caattaatac actaacgaac cggtaaacgg
      181 tcgtatgtgt tgtttaccgg gatagcgtgt gcaattcatc aacgtctctg ctaaacaact
      241 ggcaggcaaa tcacggctat tggttaacca attgctgggt gaaaagtata cgaatagagt
      301 gtaccttcgc actattcaac agcaatgata ggcgctcacc tgacaacgcg gtaaactagt
      361 tattcacgct aactataatg gtttaatgat ggataacatg cagactgaag cacaaccgac
      421 acggacccgg atcctcaatg ctgccagaga gattttttca gaaaatggat ttcacagtgc
      481 ctcgatgaaa gccatctgta aatcttgcgc cattagtcct gggacgctct atcaccattt
      541 catctccaaa gaagccttga ttcaggcgat tatcttacag gaccaggaga gggcgctggc
      601 tcgtttccgg gaaccgattg aagggattca ttttgttgac tatatggtcg agtctattgt
      661 ctctctcacc catgaagcct ttgggcaacg ggcgctggtg gtcgaaatta tggcggaagg
      721 gatgcgtaac ccacaggtcg ccgccatgct taaaaataag catatgacga tcactgaatt
      781 tgttgcccag cggatgcgtg atgcccagca aaaaggcgag ataagcccag acatcaacac
      841 ggcaatgact tcacgtttac tgctggatct gacctacggt gtactggccg atatcgaagc
      901 ggaagacctg gcgcgtgaag cgtcgtttgc tcagggatta cgcgcgatga ttggcggtat
      961 attaaccgca tcctgatcct ctcatttcgc gggctggtga taactgtgcc cgcgtttcat
     1021 atcgtaattt ctctgtgcaa aaattatcct ccccgtgttc ggagaattcc cccccaaaat
     1081 attcaccgta gccatatgtc atgagagttt atcgttccca acactcttga acgaacgttc
     1141 gattgcttat tttatggctt ctgtcaacgc tgttttaaag attaatgcga tccatatcac
     1201 gctgtgggta ttgcagtttt tggttttttg atcgcggtgt cagttctttt tatttccatt
     1261 tctcttccat gggtttctca cagataactg tgtgcaacac agaattggtt aactaatcag
     1321 attaaaggtt gaccagtatt tttatcttaa tgaggagtcc cttatgttac gtcctgtaga
     1381 aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga
     1441 aaactgtgga attgatcagc gttggtgcga aagcgcgtta caagaaagcc gggcaattgc
     1501 agtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt atgtgggcaa
     1561 cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct
     1621 gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga
     1681 gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa
     1741 aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg
     1801 aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa
     1861 ctatgccggg atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga
     1921 tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt
     1981 ggtggccaat ggtgatatca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac
     2041 tggacaaggc accagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga
     2101 aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc
     2161 gctgcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa
     2221 accgttctac tttactggct ttggtcgtca tgaagatgcg gatttgcgag gcaaaggatt
     2281 cgataacgtg ctgatggtgc acgatcacgc attaatggac tggattgggg ccaactccta
     2341 ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat
     2401 cgtggtgatt gatgaaactg cagctgtcgg ctttaacctc tctttaggca ttggtttcga
     2461 agcgggcaac aagccgaaag aactgtacag cgaagaggct gtcaacgggg aaactcagca
     2521 ggcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt
     2581 gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc
     2641 gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt
     2701 aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa
     2761 ccgttattac ggttggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga
     2821 aaaagaactg ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg
     2881 cgtggatacg ttagccggcc tgcactcaat gtacaccgac atgtggagtg aagagtatca
     2941 gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga
     3001 acaggtatgg aatttcgccg attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa
     3061 caattgacct acatgggcct cgggctttgc tacagcctgg tgaatattcc ttatggttca
     3121 cttgctaccg cgatgaccca acaaccacaa tctcgcgccc gtctgggcgc ggctcgtggg
     3181 attgccgctt cattgacctt tgtatgcctg gcatttctga taggaccgag cattaagaac
     3241 tccagcccgg aagagatggt gtcggtatac catttctgga cgattgtgct ggtgattgcc
     3301 ggaatggtgc tttacttcat ctgcttcaaa tcgacgcgtg aaaatgtggt acgtatcgtg
     3361 gcgcagccgt cattgaagat cagtttgcaa accctgaaac ggaatcgccc gctgtttatg
     3421 ttgtgcatcg gtgcgctgtg tgtgctgatt tcgaccttcg cggtcagcgc ctcgtcgttg
     3481 ttctacgtgc gctatgtgtt aaatgatacc gggctgttca ctgtgctggt actggtgcaa
     3541 aacctggttg gtactgtggc atcggcaccg cttgtgccgg ggatggtcgc gaggatcggt
     3601 aaaaagaata ccttcctgat tggcgctttg ctgggaacct gcggttatct gctattcttc
     3661 tgggtttccg tctggtcact gccggtggcg ttggttgcgt tggccatcgc ttcaattggt
     3721 cagggcgtta ctatgactgt gatgtgggca ctggaagctg ataccgtaga atacggtgaa
     3781 tacctgaccg gcgtgcgaat tgaagggctc acctattcac tattctcatt tacccgtaaa
     3841 tgcggtcagg caatcggagg ttcaattcct gcctttattt tgggattaag cggatatatc
     3901 gccaatcagg tgtaaacgcc ggaagttatt atgggcatcc gcacatcaat tgccttagta
     3961 ccttgcggat ttatgctact ggcattcgtt attatctggt tttatccgct cacggataaa
     4021 aaattcaaag aaatcgtggt tgaaattgat aatcgtaaaa aagtgcagca gcaattaatc
     4081 agcgatatca ctaattaata ttcaataaaa ataatcagaa catcaaaggt gcaactatga
     4141 gaaaaatagt ggccatggcc gttatttgcc tgacggctgc ctctggcctt acctctgctt
     4201 acgcggcgca actggctgac gatgaagcgg gactacgcat cagactgaaa aacgaattgc
     4261 gcagggctga taagcccagt gctggcgcgg gaagagatat ttacgcatgg gtacagggag
     4321 gattgctcga tttcaatagt ggttattatt ccaatattgt tggcgttgaa ggcggagcgt
     4381 attatgttta taaattaggt gctcgtgctg atatgagtac ccggtggtat cttgatggtg
     4441 ataaaagttt tggctttgcc ctgggggcag taaaaataaa acccagtgaa aatagcctgc
     4501 ttaaattagg tcgcttcggg acggattata gttatggtag cttaccttat cgtattccgt
     4561 taatggttgg cagttcgcaa cgtacattac cgacagtttc tgaaggagca ttaggttatt
     4621 gggctttaac gccaaatatt gatctgtggg gaatgtggcg ttcacgagta tttttatgga
     4681 ctgattcaac aaccggtatt cgtgatgaag gggtgtataa cagccagacg ggaaaatacg
     4741 ataaacatcg cgcacgttct tttttagccg ccagttggca tgatgatacc agtcgctatt
     4801 ctctgggggc atcggtacag aaagatgttt ccaatcagat acaaagtatt ctcgagaaaa
     4861 gcataccgct cgacccgaat tatacgttga aaggggagtt gctcggcttt tacgcgcagc
     4921 tcgaaggttt aagtcgtaat accagccagc ccaatgaaac ggcgttggtt agtggacaat
     4981 tgacctggaa tgcgccgtgg ggaagcgtat ttggcagtgg tggttatttg cgccatgcaa
     5041 tgaatggtgc cgtggtggat accgacattg gctatccctt ttcattaagt cttgatcgta
     5101 accgtgaagg aatgcagtcc tggcaattag gtgccaacta ccgtgtaact ccgcaattta
     5161 cgctgacatt tgcaccgatt gtgactcgcg gctatgaatc cagtaaacga gatgtgcgga
     5221 ttgaaggcgc gggtatctta ggtggtatga actatcgggt cagcgaaggg ccgttacaag
     5281 ggatgaattt ctttcttgct gccgataaag ggcgggaaaa gcgcgatggc agtacgctgg
     5341 gcgatcgcct gaattactgg gatgtgaaaa tgagtattca gtatgacttt atgctgaagt
     5401 aagaaataac gccggagaga aaaacctccg gcgtttcaga ttgttgacaa agtgcgcgtt
     5461 ttttatgccg gatgcggcgt aaacgcctta tccagcctac aaaaactcat aaattcaaag
     5521 tgttgcagga aaaggtaggc ctgataagcg tagcgcatca ggcaatctct ggtttgtttt
     5581 cagatgaaaa cgccggagtg aaaattctcc ggcgttttgg ccgtgaatta ctgctgcgga
     5641 attgccggta cagccggaac gttaagagct ggcatcgcaa acatgccaac aaaatcttct
     5701 aacgacattt tctgcccatt taacgttatc tgaccgttag catattgcag gctggtggtg
     5761 atggtattgt cctgcaaggt ggtcagacgg aacatctgcc ccattgctga tgcaccttca
     5821 acttgctgtt tcgccagttt tttcgcttga tcttcctgat aaccttccag cttcgctacc
     5881 tgagtcatca actcagttgc catatccacc ggaatggtca gtttcgcatc cagagattta
     5941 accgaacgat ctacttcctg cgccagcgtt tgcggcgctt ctttagtcgt tgccggatct
     6001 ttcaggaaca gcgacagatt cagcgcactt tcaccctgac tgtttttcca gcttagcggc
     6061 gcgatagtaa tcaccggatc gcctttcaac atcagcggca gggcgctaaa gaaggcttcc
     6121 gtcactttct cctgataaag ttcgggattg ttggcaattt ctggctgtgc cagcagcgcc
     6181 tgagtttgcg cgttatattg ctggctaaac tgatgccagg cttcaccatc aatctggccg
     6241 acttttaaag tcagcttgcc gctgcccaga tcctgattct gtaccttcag gctgtttagc
     6301 gagtaatcca gttggctatt gatcgtttta ccgtcattga ccagatccga tttaccgctg
     6361 atctccatgc cttccagcag tgccagttct ttgccttcca ctgaaatggt cattttttcc
     6421 agtgacagtt tttggtttcc tacgcgctca ccaaaacttc ccagcgtgct ggaaccgtcg
     6481 gttttcagat tattaaaggt caactgcact ttctggttgt attcgttaac tgcgtctatc
     6541 cgaccacttt gcgcctctcc ggaaagggag atggctttgc cgtctctgtc agcatttaac
     6601 tggaactcgc cgccgctaaa ggcgactttt tcatcctttt gctcgtaatt cagtggattg
     6661 agcgaaatat cggaactgga atcaccgctg taaccaatgc gcgagttaat ctcaaaaggc
     6721 gtttcacctt ttgccatatc aaacagtggt ttgcttactt cgttattaac cagcgtggtt
     6781 tgaattgatg ccatcgacgg gatcaggttc agttttttaa gctgggcaag cgggaaggga
     6841 ccatgatcaa ccgattcgtt gaagatgacg ctctgaccgc ttttaatcca cggattttct
     6901 ttcccggcaa tgggtttcac caacagttgc aactggctgc tgaatacgcc gcgatgatag
     6961 ttttgataac tcacttccag gttggattca ggagctgtca gtttgagttg cgcgttcgcc
     7021 tgcgcgacca tgtcttcgag atgggtttca atcttcttgc ctgtatacca tgcgccgcct
     7081 gtccagacta cgcctagcgc aacaatgacg cctaccgcta ccagcgattt attcataatg
     7141 attatccata aaatgaaatc aggcggactg gccgcctgaa ggtgttataa gcctttaata
     7201 agcttagcaa gagatgttaa ttttttcagt aagctcttac agcttgttgt aaacacgcgc
     7261 taaacggccg tggcctttga cagtcaccgg tgattcgttg gcggcaataa acgctgattc
     7321 acccggttta agctgtaact gctgagaacc tttccacaac gttgcatcgc cttcgacgca
     7381 gaacaaaatg gcggcactct gctggctgat ggtggtttct ttatcactaa ggtcatgcag
     7441 cgagaaggca aaatcatcca caggaatcgg gaagtccagt tctgcacctt gtttcaccgg
     7501 ctgggtcaac aactggttag ccggtttggc ttcgaatttc acattggcaa ccagttccgg
     7561 aatatcaatg tatttaggcg tcagacctgc acgcagcacg ttatcggagt tcgccatcac
     7621 ttccagcgcc acgccttgca ggtaagcgtg cggtgtttca gcgaacagga acatcgcttc
     7681 gccaggattc aatttcacca cattcagtaa cagcggggag aacaggccgc tgtcttccgg
     7741 gtaaaattca gaaattaaac gaatcgtttg ccacggttca ccctgctgac tgtcgagcgc
     7801 cgattttaaa atcgccagcg cgcgggattt ttcttcaccc tgcatattca acaggttggc
     7861 gaacagttcg cttaaacgtt cggcatgtgg ctgttgtaaa aagtgagcaa tcgccggatg
     7921 tgcaccggcg accggctgga gtagggagac aatctcggaa aattcacgaa acgcgttcat
     7981 cgcaaggaaa ggcgtcagcg caaaaaccag ctccggcttg tggttaggat ctttatagtt
     8041 acgctcggcg gcatccatcg ggatacctgc ggcattttct ttggcaaaac cgatttcaga
     8101 attgtgtttg tttggatgaa cctgaatgga gagtggctgt gctgcgcata atactttgaa
     8161 caggaaaggc agttcgccaa agcgtttggc aacggcctct ccgagcagag tcgatttatc
     8221 actctcaatc acatcacgca gtgaaacgat atctccggcg gcattctgca ctcgtgaact
     8281 gcttttcgga tgtgcgccca tccacagctc ggccatcggc tggctggacg gattttccat
     8341 accataaagt tcagtcaacg ccgttttgct accccaggca tagttttgca ctgagttaat
     8401 gagtttttgc atgatcaatc cctgttttaa tgtggaaatt aatcccgcta ttaaagcaag
     8461 aatcctacgg gaagtaacct ggagccgcaa aaagtcgtac tagtctcagt ttttgttaaa
     8521 aaactgtgta ggatattgtt actcgctttt aacagggcaa cggaacaccc gcccagagca
     8581 taaccaaacc aggcagtaag tgagagaaca atgtcaaaca aaccctttca ttatcagtct
     8641 ccttttccac tcaaaaaaaa tgatactgag tattacctgc taaccagtga acacgttagc
     8701 gtatctgaat ttgaagggca ggagattttg aaagtcgcac ccgaagcgtt aactctgttg
     8761 gcgcgtcagg cgtttcatga tgcgtcgttt atgctgcgtc cggctcacca acaacaggtg
     8821 gccgacattc tgcgtgaccc ggaggccagc gaaaatgata aatatgtggc gctgcaattc
     8881 ctgcgtaact ccgacatcgc ggcgaaaggc gttctgccaa cctgtcagga taccggcacc
     8941 gcgattattg ttggtaaaaa agggcagcgt gtatggaccg gtggtggtga tgaagcggcg
     9001 ctggcgcgcg gtgtctataa cacttatatc gaagataatc tgcgctactc gcaaaacgcg
     9061 ccgctggata tgtataaaga agtgaatacc ggcaccaatc tgccagcgca gatcgatctt
     9121 tatgccgttg atggcgacga atacaaattc ctctgtatcg caaaaggtgg tggttcggca
     9181 aacaagacgt atctctatca ggaaaccaaa gcgttactga cgccggggaa actgaaaaat
     9241 tacctggttg agaagatgcg cacgctgggt accgcggcct gtcctccgta tcatattgcg
     9301 ttcgttattg gtggaacttc tgcagaaacg aaccttaaaa cggtgaaact ggcttccgcg
     9361 aaatactatg atgaactgcc aacggaaggg aatgagcacg gtcaggcgtt ccgcgatgtg
     9421 gaactggaaa aagaattact gatcgaagcg caaaatctcg gtctgggtgc gcagtttggt
     9481 ggtaaatact tcgctcacga catccgcgtg attcgcctgc cacgtcacgg cgcatcctgt
     9541 ccggttggta tgggtgtttc ctgctctgct gaccgtaata tcaaagcgaa gatcaaccgt
     9601 caggggatct ggatcgaaaa actggaacat aatccaggta aatatatccc tgaagagctg
     9661 cgtaaagcgg gagaaggcga agcggtgcgc gttgacctta accgtccgat gaaagagatc
     9721 ctcgcacagt tgtcgcagta tcccgtttct acacgcttat cgcttaacgg cacgattatc
     9781 gttggtcgtg atattgctca cgccaaactg aaagagcgga tggataacgg tgaagggctg
     9841 ccgcaataca ttaaagatca tccgatttac tacgcgggtc cggccaaaac gccggaaggt
     9901 tatgcctccg gttctcttgg cccaacgacc gccggacgga tggattctta tgtcgatcaa
     9961 ctgcaagcgc agggcggaag tatgatcatg ctggcgaaag gcaaccgcag ccagcaggtg
    10021 acggatgcct gtaaaaaaca cggcggcttc taccttggca gtatcggtgg tccggccgct
    10081 gtattggcgc agggaagtat taagagcctg gaatgtgttg aatatccgga actgggaatg
    10141 gaagccatct ggaaaattga agtggaagat ttcccggcgt ttatccttgt ggatgataaa
    10201 ggaaatgact tcttccagca gatacaactc acacagtgca ctcgctgtgt gaaataaaca
    10261 gagccgccct tcggggcggt ttttttacat ggcacgaaag accaaacatt tgttatcaaa
    10321 tagtaaataa tgagtgagct aaaagttgct taacgaaagc aaaacagaaa gaaaaaatta
    10381 atcaggtgag gagcaggcca tgaatacagt acgcagcgaa aaagattcga tgggggcgat
    10441 tgatgtcccg gcagataagc tgtggggcgc acaaactcaa cgctcgctgg agcatttccg
    10501 catttcgacg gagaaaatgc ccacctcact gattcatgcg ctggcgctaa ccaagcgcgc
    10561 agcggcaaaa gttaatgaag atttagactt gttgtctgaa gagaaagcga gcgccattcg
    10621 gcaggcggcg gatgaagtac tggcaggaca gcatgacgac gaattcccgc tggctatctg
    10681 gcagaccggc tccggcacgc aaagtaacat gaacatgaac gaagtgctgg ctaaccgggc
    10741 cagtgaatta ctcggcggtg tgcgcgggat ggaacgtaaa gttcacccta acgacgacgt
    10801 gaacaaaagc caaagttcca acgatgtctt tccgacggcg atgcacgttg cggcgctgct
    10861 ggcactgcgc aagcaactca ttccgcagct taaaaccctg acacagacgc tgagtgaaaa
    10921 atcgcgtgca tttgccgata tcgtcaaaat cggtcgaacc cacttgcagg acgccacgcc
    10981 gctaacacta gggcaggaga tttccggctg ggtagcgatg ctcgagcata atctcaaaca
    11041 tatcgaatac agcctgcctc acgtagcgga actggctctt ggcggtacag cggtgggtac
    11101 tggactaaat acccatccgg agtatgcgcg tcgcgtagca gatgaactgg cagtcattac
    11161 ctgtgcaccg tttgttaccg cgccgaacaa atttgaagcg cttgcgacct gtgatgccct
    11221 ggttcaggcg cacggcgcgt tgaaagggtt ggctgcgtca ctgatgaaaa tcgccaatga
    11281 tgtccgctgg ctggcctctg gcccgcgctg cggaattggt gaaatctcaa tcccggaaaa
    11341 tgagccgggc agctcaatca tgccggggaa agtgaaccca acccagtgtg aagcattaac
    11401 catgctctgc tgtcaggtaa tggggaacga cgtggcgatc aacatgggtg gcgcttccgg
    11461 taactttgaa ctgaacgtct tccgtccgat ggtgatccat aatttcctgc aatcggtgcg
    11521 cttgctggca gatggcatgg aaagttttaa caaacactgc gcagtgggta ttgaaccgaa
    11581 tcgtgagcga atcaatcaat tactcaatga atcgctgatg ctggtgactg cgcttaacac
    11641 ccacattggt tatgacaaag ccgcggagat cgccaaaaaa gcgcataaag aagggctgac
    11701 cttaaaagct gcggcccttg cgctggggta tcttagcgaa gccgagtttg acagctgggt
    11761 acggccagaa cagatggtcg gcagtatgaa agccgggcgt taatctgcaa catacaggtg
    11821 cagccgtgga atgatcaaac gaagcggctg cgcctgaggt ttataacggt gctgcacatt
    11881 gtcggcatcg taatttaaca actcaccaac gtccggcacg cccgcgccat tatcccgatt
    11941 aatcagtgca atcagtggtg tagggcaggc gtgttggact tgtttttgat ctcctttgta
    12001 ccagacgcgg gcaatcggct gcaccttcac tggacgtttg atttttaact tcgcgttctg
    12061 tggcagggca gcgatatcct gatactctcg ctccagtttt ctttgccact cctcgcgcgt
    12121 ccacggtgcg acactgcgtg gtgatttcag gcttttttcc agctgtgcca ggacttcatc
    12181 acgatgcaaa ttcttaatga tatgtttatt agcccaacca aagcgtaaag tggcggggtc
    12241 gtgcagaacg gtgagcgtgc ggtaagcatt aagggtgatc agccccggca aatgacgatg
    12301 cacccattca aaacgtgccg cggtggggag ttctgattca accgtgacga tatgctcgaa
    12361 cgtggtcttg agtttattga tgtgctgaat atgactgacc aacgctgctt gcgaaaggtt
    12421 atcgacctgg taacacaaca cgccaggcag acggacagcg gccttgctgc tgcgattttc
    12481 ggactgttgt tgaataaata aatggcggaa atgacgcaac gccagcgact gcgcgtcgtt
    12541 gccgagatgt tgttttacct caatacgatt aagcggatta tgctcatcct ctttttttac
    12601 ctccggcaaa gagaacacgc gggcaaccaa tagcttgtgt tgctcaagat gagcggcaaa
    12661 tgtagccagc tcttgttcca tctggcgaaa ggtagtgttg agtcggtcta cgagatcgta
    12721 acgcgccata atattaacca ctttagttac aacatactta ttttatagca cagtcgtggt
    12781 gacttgacca tcgtgcgcgt agtgtcaggc agaggtaaat tgcgggatgt tatgccataa
    12841 cggccagcta aacgagaagc gagcaccacc cagttcgctg atgtcacaat taaccgtacc
    12901 gcccattgcc agtgctattg agtggacaat tgccagcccc agcccgcagc cgccggttga
    12961 gcgatcccgg ctgggatcga ggcgaacaaa aggttcaaag atatgttcgc ggttttctgg
    13021 ggcaatccct gggccatcat cctcaacaat taatgtcgct ctattccccg acagtagcag
    13081 gctggtttca accgttgaat ggcagtagcg cagggcgtta ttgagcaaat tatccagcac
    13141 gcgctccatt aagcgcatat ccaacgccgc ataatggcct tgcatgagcg tttttatccg
    13201 taccgtttta tcgggcgtta ctgcctgaat atctgccaga tgcgttgaca gccacaacgg
    13261 caggtctggt tcgctaagat gaagctcgtt ttgtgggcga tcgagtcggg cataagtcag
    13321 cagctcttca attaaagctt caagttgact gatatcacga ttcaacgcct gggattcggc
    13381 ggcgctcagg ttatcgctca tctccagtcg ataacgcagg cgcactaacg gtgttcgcag
    13441 ttcgtgagcg ataccgtcaa taagctgttt tttgctggca attaaggcgt tgatattgtc
    13501 cgccatctgg ttaaatgcga taccaagtcg ttcaaagctc gaaccctcat caaagtggat
    13561 acgttcattg agatgcccat cgccaaatcg ttgcgccgct gcttccagtt ttaacatatc
    13621 ctgccagtgc ggacgcatcc agataaacac cggaaaggcg agggaaatag caataaaagc
    13681 gatcagggcg atatccagca atcgcatttg atggaggtaa taaagataag gaacaggact
    13741 aactgccagc acgtagtggc tgcgcgggat acgctgcaaa aacgtgtact gatcgtccag
    13801 ggcgacaatt tcgccgccac gcagtcggtg catggaaata tcatcaagat ggtatttact
    13861 cagtggctcg acacgcagat cgaaagagag atttaaatcc atctctttca gcgtcttacc
    13921 ccagtcgtgt ggggggatct ctcgcaattc gctgcgcatc agatacagcg aactgttcat
    13981 caaatcatcc agcgactgtt tgcccgcgcg ttcggcggta aatttgtaca ccagcccaac
    14041 cagcagagac atcacaagga agcagacaaa caataacagg taaaactgga taaacaattt
    14101 tttcattggc atcgcccgca acagaatact attcgtatta attcacgact gtggagcaat
    14161 gtaaaccgcc aactggataa gaccttgttt ggaagataat gtccggaaca gacggatgta
    14221 tccccggtgt attcatattc agtaaattca attttccacc ggggcttgtt tattatgcta
    14281 aggtttaatt taacgtgacg ctttgttgtt ctttagtctc tctatcctta agaagacggg
    14341 ctgatattac accggacatt attgaaccat tcacattaag cgctgttctt gccatatcaa
    14401 cgatcggctc tatggcaata aaaataacta ctaatgcgac agggaaatta agtgttgaaa
    14461 ggacaatcag cgccgcgaat gtcccaccac tacctacgcc tgctacccct attgagccaa
    14521 gcgcgatggc aggtaaaaga gttgcgagga attgcaatga gaagggatcg atacccattg
    14581 ttggagcaat catcgccacc atcacggcag gataaatccc agcgcagcca ttttgtccca
    14641 tgctggaacc aaaagacgct gcaatattag caatcgtatt tggaaccccc aatttttcca
    14701 atgcagaaat agccagagga attgaagccg cactactgcg agaaacaaac gcaaaggtaa
    14761 gtacttgcca gacggtcttg taatagttaa cggggttttt acctattaac agcaaaatgc
    14821 ccgagtgaac aataaacatc acaaaaacgg ccagataaca ggcgacgatg aacccgagca
    14881 aattggtaaa ctgtgaaagc tgatacgttg agaagacttt cgccattagt gccattaccc
    14941 cataaggcgt cagcgcaatg acaatacgga ccattttcat tacccatatt tgaatcgcgt
    15001 tgatccccgc gctcaatttt tcgccctctg ctggtgcatc tttctttact tttaacagcg
    15061 caataccagc aagaattgta aagatgacga tgccaataac cgaaacactt cttgccccgg
    15121 tcagatcaag gaagaggttg gttgggatca atgacgtaat gagtcgcggt aaagataccg
    15181 cagctgtttt cgagacgtca tcgctggtta aagaggattg catatgacca aaggcgctgg
    15241 catcaaggcc aattgcactg gcaatagcca ggccaactaa acctgcaatc atgactaaac
    15301 acaacataca ggcaacaata gttaaagaca tcttgccaat accagcgcta ttttcgagct
    15361 tattaatcgc tgaaagaatc gaaatgaata tgagtggaac ggcaacaagt tttaataaat
    15421 taacataacc gttaccagca atattatacc attcgataac ggtttcactg cttgtctcag
    15481 gaatgaaatg cagggagata ccaatgatag cccccaggaa aatcgcgata aaaacacttt
    15541 tggaaaggga ttcgctcttt tgatccgttt tgaaaatccc ccatgagcag atcaaaaaaa
    15601 gcacaatata tgtgaaagtt gtaaccgttt tttaagcctc ttgcctcatt cagcacctga
    15661 aatgccggaa agagtttgtt gtaaaaaatc tgcgccataa gacgcaggga cgaggcgatt
    15721 atagagaaat tttttctcat tttcatatca aatgtagggg gtttggaatt tgaataagct
    15781 cttattaatt gaattcgtta attgttctct ttttcagatg aacaaatatt agtcatctga
    15841 ttgtttattg tcactatccc atgcatgagg cgcaaaaaga tagcctttgt tacgcacagt
    15901 tttaatgcga taaggttctg cggcgttatc gagcagtttt tttcttaacc gcgaaatagc
    15961 cacgtccacg ctacgatcca gtccgtcata actgacgccg cgtaaatttt tcagcaatgc
    16021 atcgcggtcc atgatttgcc cggcatgcgt agctagttcc cacaataatt cgaaatcagc
    16081 tgtcgagagc gagatttcag tgttagccag ggtgactacg cggttgatgg ggtcgatagt
    16141 taacgtgccg aaatgcaggg ctttgtaggg agtaagagac gtttcctgaa gacctttggt
    16201 cagtgtggct tgctcattct gacgcaaatg caaacgtaaa cgcgctagca aaacagcagg
    16261 gggcgtcgtt ttgagaatat agtcgcaggc acccatttcc agtgccagga tgtggttcat
    16321 atcgctatcg agagaggtta gaagaacaat cggtccagac cactttgcgc gtaaatcacg
    16381 acaaatggtc atgccgtcct tgcctggtag catgatgtcg agtaacacca aatccggatt
    16441 ttctcgcaaa atggtttctt cggcctggtc gccgcgcggt tctacggtaa cctgcatatc
    16501 atgttttgcc aggtacgcgg caatcagtga accgacttcc gcatcatctt ccacaaatac
    16561 gatagtgttc ataacattca cggtagatat aaaaacgtca caatacaccg cgctgttttt
    16621 acttactatc aatcgtttct aaacaagttt tcatcaggta ttctactgat gacttgttat
    16681 ggaagtgtta aggtaaaaag atggggctgg taatcaaagc cgcgctcggt gcgctggtag
    16741 tgctgttgat tggtgtttta gcaaaaacga aaaattatta tatcgccggg ctgattccac
    16801 ttttcccgac ctttgcgctt atcgcgcatt atattgttgc cagcgaacgc ggcattgaag
    16861 ccttacgcgc aaccatcatt tttagtatgt ggtcgattat tccctatttt gtttacctgg
    16921 tgtcgctgtg gtatttcacc ggaatgatgc gcttgcccac cgcttttgtt ggttctgttg
    16981 cgtgttgggg gataagcgca tgggtgttga ttatatgctg gataaagctg cattaacgca
    17041 gatgacgacc gccatcaagt gggaaactgc gtccggtgac aaagcaactg gtaagtaagt
    17101 aatcgaccag gtcgatcact tctttctcgc caggcgcggt tttcatcagt gatttattca
    17161 gcgcctgttg tcgatattcg gcatcatcat gttcattaaa caggatcagc gatggcgcaa
    17221 tagaattcac tttcacttcc ggtgccagct tgcgggcaaa cgagcgggtc atattatcca
    17281 gtgccgcttt gcttgcagca tacgcaacat gtttgtcgct accgcgctcc accacataat
    17341 cggtaaagtg aatgatatcg ctggcggcgt gtccgtgccc acgcagtaat ctttccagcg
    17401 catggttgag caggtatggg gtattaacgt ggatctgcat catgcaagcc agtacgtcgg
    17461 ccagtggcgc acccggtttt tccgcaatcc acgcactggc gttatgcaaa atagcacgca
    17521 gaccatgggt ggtttttagt acttcatcgg caaacgccat cacaccgtcg ttggtcgaaa
    17581 aatcagcctg aatacactgc gcacctgcat taatcagtcc atcaatggct ggatagtgtg
    17641 tccgatagct gacaatcacc ggttgctttt gattaatgaa atgccatgcg agggcgaggc
    17701 cgatgcgacg acctccgcca gtaattaata ttggcaaggg ctgggtttta cccatcgtta
    17761 tctcctttgc tatccaacga tgggatgaac gttatcccac cagcatccag gtcgccggaa
    17821 ctgaggcaat taacagcatg ccaatcagca ccatttcctg acggttcagt acattatcat
    17881 gtgtatgcgt tttgcgcgca tacaggaaaa ccagcagccc cggtgcatac agaacaacgg
    17941 ataacagcaa gtgcattgga ccagaggcgt acaataacca taagccataa atgcaggcac
    18001 cgacacctac cgctttatgc agtggacggg ttgcgatttt cagcaagaac gcgccgacca
    18061 ggaaataggg caccagaatc atttctgagg cgatggtcag caacgtgttg taatcggaac
    18121 cggtcagcca gatgagtacc agacaaattt gcacgcagat gttggtcaac cacagtgagg
    18181 cggatggcgc agcttgtgcg ttctggcgcg caaaaatgcg tgggaatgct ttatgagtgg
    18241 cagccaggaa cggaacttct gccgccatga tggtccagct taagtacgcg ccgcaaacgg
    18301 aaacgatcaa accggcagcg atgatgattt cgccccatgg tcccatcatt tccaccatca
    18361 gaccggccat tgacgggtta cgaatttcag ccagttcagg acgtgccacc acacccagcg
    18421 aaagcagcgt taccagcaag taaacgccca gagcggagag aaccgccagc agtgtcgctt
    18481 tgccaacatc acgtttatta cgcgcacgcg cagaaacaac cacggcacct tccacaccaa
    18541 tgaaaaccca cagggtgatc agcatggtgt ttttcacctg ttcccaaaca ggtacgccaa
    18601 gtgcaagtcc ggtgaagtcg agcttgaagg tatccagttt gaacatcatc atcgccagca
    18661 caacaaacag acccagcggc aacaattttg ccagtgtcgc caccaggtta atgctggcag
    18721 cggtttgcac cccgcgcagg atcaaaaagt gaacaatcca caataaagcc gatgcaccga
    18781 ctatcgattg ccaggtatta ccatcgccaa acaggcgcaa ttccggcgtg tccgtaaaaa
    18841 agcttaacgc ggaaaaaacg atcaccagat aggagacgtt ggcgatgacc gcgcacagcc
    18901 agtatcccca tgcggaacaa aagccgatta gctcgccaaa cccttcgcgg gcataggtaa
    18961 agataccgcc atcaagttcg ggacgaatgc gcgtgaggat cagcatggca aaggccagca
    19021 ataaaatgcc agcgccagta ataccccagc cgatgagcag tgctgccggg ctggcaactg
    19081 ccgccatatt ttgcggcaga ctgaaaacac ccgcgcccag cattgagctt aataccagcg
    19141 cggtgagtgc gctcagtccc agtttctttt ccatcgatat ccagtgatta aaacgtgata
    19201 agaagaataa atattagggc aaaacgcata aaaatggagt ttgccactga agcgcgggat
    19261 tttacgaaga gatgtgtctg catgcaatgg tcgcggggaa atttgtgact aaaaatgcga
    19321 aaggcggcat caagccgcct tataggagta actgaatagt tatttataca gatctgcgct
    19381 gacggtcagg ttattaccac gtttctgcca ctggcgggtg atgtggtaat acttagcacc
    19441 tttcttcgcg gcacgtttcg caacctgata ggagacttcg gtcatgttgc cgtagttgcc
    19501 agagaatttg atgctgtcga acgggaccat catcgctgct gtcgctttgt tcagttcttc
    19561 gactttagtg ccatccggga gcgtgacggt gtaacgcccg ccttttgatg actgggtttc
    19621 aaagaagcga ccgacttcag aacttggtga tgcggtagtc gcaacacccg ggatctcaac
    19681 tttcttcgcg gcttcgccac cggcagccag agctgcacgt cctgcttcgg aatctgccgg
    19741 gatcacatcc gggctctgga cgatacgttt cttagcatct tttttataga tgaatgcagt
    19801 aatacgctgg ttgccgccct ggttggcatc gatttgacga acgatgtaga aagagtaggc
    19861 acctttcgct tttgccgctt tggtgatggc atcattgact tctggctggc tacgatagaa
    19921 gccctggacg gtgaccgtgt caaacggttc aatcagaaca gcctgatctt tcggcagttc
    19981 gacaacaccg ttaattacgc gattacttgt ttcttctgct ttttcagcat cggctttata
    20041 gaggtcagcg accacacgcc agttaccgct gttaccaaaa tcagaagtgt cgacaacata
    20101 aaaagaggcg gcaccttctt tatctgcgcg acgagaaacg gctttcaccg cttcgccaat
    20161 agcattaaaa cgaccggtaa ccactacacg gtcaaaaggt ttaaccgctg ccgcttgctc
    20221 cggtgtcagt tctgttgctg cgttaacgga gaatgccgta gcagaaagca gtgccgacgc
    20281 caggagggtg ttcttaagct tcataaaaat aatccttcgc cttgcgcaaa ccaggtactg
    20341 gtattgttat taacgagaaa cgtggctgat tattgcattt aaacggtgta actgtctgcg
    20401 tcatttttca tatcacattc cttaaaccaa ttttaatcct gctcaaatga ccgtctatgc
    20461 ttaaaaaaca gccgtatcag catcattact actgaagcaa ctgaattgta taagttaatt
    20521 taatgttaag tagtgattcg tgccggggcg atgtctcgtt ttacccgacc gtcgaagaca
    20581 attatcagtc tttatccggc gttctaaggt gtttatccca ctatcacggc tgaatcgtta
    20641 atattttgcg agttcacgcc gaaatactga tttttggcgc tagatcacag gcataatttt
    20701 cagtacgtta tagggcgttt gttactaatt tattttaacg gagtaacatt tagctcgtac
    20761 atgagcagct tgtgtggctc ctgacacagg caaaccatca tcaataaaac cgatggaagg
    20821 gaatatcatg cgaattggca taccaagaga acggttaacc aatgaaaccc gtgttgcagc
    20881 aacgccaaaa acagtggaac agctgctgaa actgggtttt accgtcgcgg tagagagcgg
    20941 cgcgggtcaa ctggcaagtt ttgacgataa agcgtttgtg caagcgggcg ctgaaattgt
    21001 agaagggaat agcgtctggc agtcagagat cattctgaag gtcaatgcgc cgttagatga
    21061 tgaaattgcg ttactgaatc ccgggacaac gctggtgagt tttatctggc ctgcgcagaa
    21121 tccggaatta atgcaaaaac ttgcggaacg taacgtgacc gtgatggcga tggactctgt
    21181 gccgcgtatc tcacgcgcac aatcgctgga cgcactaagc tcgatggcga atatcgccgg
    21241 ttatcgcgcc attgttgaag cggcacatga atttgggcgc ttctttaccg ggcaaatcac
    21301 tgcggccggg aaagtgccac cggcaaaagt gatggtgatt ggtgccggtg ttgcaggttt
    21361 ggccgccatt ggcgcagcaa acagtctcgg cgcgattgtg cgtgcattcg acacccgccc
    21421 ggaagtgaaa gaacaagttc aaagtatggg cgcggaattc ctcgagctgg attttaaaga
    21481 ggaagcgggc agcggcgatg gctatgccaa agtgatgtcg gacgcgttca tcaaagcgga
    21541 aatggaactc tttgccgccc aggcaaaaga ggtcgatatc attgtcacca ccgcgcttat
    21601 tccaggcaaa ccagcgccga agctaattac ccgtgaaatg gttgactcca tgaaggcggg
    21661 cagtgtgatt gtcgacctgg cagcccaaaa cggtggcaac tgtgaataca tcgtgccggg
    21721 tgaaatcttc agtacggaaa atggtgtcaa agtgattggt tataccgatc ttccgggccg
    21781 tctgccgacg caatcctcac agctttacgg tactaacctc gttaatctgc tgaaactgtt
    21841 gtgcaaagag aaagacggca atatcactgt tgattttgat gatgtggtga ttcgcggcgt
    21901 gaccgtgatc cgtgcgggcg aaattacctg gccggcaccg ccgattcagg tatcagctca
    21961 gccgcaggcg gcacaaaaag cggcaccgga agtgaaaact gaggaaaaat gtgcctgctc
    22021 accgtggcgt aaatacgcgt tgatggcgct ggcaatcatc ctgttcggtt ggctgtcaag
    22081 cgttgcgccg aaagagtttc ttgggcactt caccgttttc gcgctggcct gcgttgtcgg
    22141 ttattacgtg gtgtggaatg tatcgcacgc gctgcataca ccgttgatgt cggttaccaa
    22201 cgcgatttca gggattattg ttgtcggagc actgttgcag attggccagg gaggctgggt
    22261 tagcttcctt agttttatcg cggtgcttat agccagcatt aatattttcg gtggcttcac
    22321 cgtgactcag cgcatgctga aaatgttccg caaaaattaa ggggtaacat atgtctggag
    22381 gattagttac agctgcatac attgttgccg cgatcctgtt tatcttcagt ctggccggtc
    22441 tttcgaaaca tgaaacgtct cgccagggta acaacttcgg tatcgccggg atggcgattg
    22501 cgttaatcgc aaccattttt ggaccggata cgggtaatgt tggctggatc ttgctggcga
    22561 tggtcattgg tggggcaatt ggtatccggc tggcgaagaa agttgaaatg accgaaatgc
    22621 cagaactggt ggcgatcctg cacagcttcg tgggtctggc ggcagtgctg gttggcttta
    22681 acagttatct gcatcatgac gcgggaatgg caccgattct ggtcaatatt cacctgacgg
    22741 aagtgttcct cggtatcttc atcggggcgg taacgttcac gggctcggtg gtggcgttcg
    22801 gcaaactgtg tggcaagatt tcgtctaaac cgttgatgct gccaaaccgt cacaaaatga
    22861 acctggcggc tctggtcgtt tccttcttgc tgctgattgt atttgttcgc acggacagcg
    22921 tcggcctgca agtgctggca ttgctgataa tgaccgccat tgcgctggta ttcggctggc
    22981 atttagtcgc ctccatcggt ggtgcagata tgccggtggt ggtgtcgatg ctgaactcgt
    23041 actccggctg ggcggctgcg gctgcgggct ttatgctcag caacgacctg ctgattgtga
    23101 ccggtgcgct ggtcggttct tcgggggcta tcctttctta cattatgtgt aaggcgatga
    23161 accgttcctt tatcagcgtt attgcgggtg gtttcggcac cgacggctct tctactggcg
    23221 atgatcagga agtgggtgag caccgcgaaa tcaccgcaga agagacagcg gaactgctga
    23281 aaaactccca ttcagtgatc attactccgg ggtacggcat ggcagtcgcg caggcgcaat
    23341 atcctgtcgc tgaaattacc gagaaactcc gcgctcgtgg tattaatgtg cgtttcggta
    23401 tccacccggt tgcggggcgt ttgcctggac atatgaacgt attgctggct gaagcaaaag
    23461 taccgtatga catcgtgctg gaaatggacg agatcaacga tgactttgct gataccgata
    23521 ccgtactggt gattggtgct aacgatacgg ttaacccggc ggcgcaggat gatccgaaga
    23581 gtccgattgc tggtatgcct gtgctggaag tgtggaaagc gcagaacgtg attgtcttta
    23641 aacgttcgat gaacactggc tatgctggtg tgcaaaaccc gctgttcttc aaggaaaaca
    23701 cccacatgct gtttggtgac gccaaagcca gcgtggatgc aatcctgaaa gctctgtaac
    23761 cctgacggcc tctgcggagg ccgtcactct ttattgagat cgcttaacag aacggcgatg
    23821 ctttgacctc ccgctgtttg ttcaagcgca attttgacaa taattgtcaa cggcacggaa
    23881 agcagcatac ccaccggtcc taacaaccat ccccaaaaaa tcaacgacaa aaataccacc
    23941 aatgtcgaaa gccccagccc acgtcccatg atgcgcggct ccagaatatt gccgaagacc
    24001 agattaatca gcagatatcc cgccagcacc agcaacgctt cgtagaagcc attaaacacc
    24061 agtacctgag cgataggggg gattgccgcg agaactgaac caatattagg gatgtaatta
    24121 agcgcaaagg ccagcaatcc ccagacaaaa gcgaagcgaa catcgagtgc ggcgagcatc
    24181 gcccaggcga ccaggccggt gatgatgctg atggctgttt tcagcaccag ataatgagaa
    24241 acactgtcaa tcgcacgttg aatcgccgcc atcccttcaa ccggacgcgc catcatttgc
    24301 tgaaattttc cgggcaattg tggcacttcg agcagcataa acagcaccgt cagcagcaat
    24361 aaaaatattg atgacatggc attagataac tgcgtcaata agttggtgag caacgtcatc
    24421 gccgcgttcg gatcaatgta atgcgccagt tggtcaactg agacgtcaat ccctacgcgt
    24481 tgcaacaacg gctcaagagc ttgcagcggc gtcataatag agttgcgata ttgcggtaac
    24541 gtccacgtca actcgttgag cgcggaaccc agataagcta atagcaacac catcgccatc
    24601 acgatgattg tcatcaaaat cgacactgcc agtacacgtg gcacacgcca gcggaccata
    24661 tgttgcacca gcgggttaag aataacagca ataaataatg cgagaataaa cggcacgatg
    24721 atctcggcgg caaaacggat accgcagaga ataatgacca gcattcccaa cataatgacg
    24781 atttttaggc cattgagcgt gatgatcggc tttgccatgc ttgtccccgt ttttcttcct
    24841 ttgctaataa taatgtcttt ttcgctccgt catcataacg aaaatcaaac cgctcaggta
    24901 aagaagtgaa aatattttga gttaattctt aagctatgat acaaatcagg cgtgttcaac
    24961 taccgaggac aattatcatc tgcgatgacg agaagcaaca ctgcggataa ttgtaatatt
    25021 atggacaatt tgttcaggac gttattttct acatttaccc atcttcgtac ctcatccact
    25081 atcttgctcg taggtgagca gcactggcgc aacgcgcttt agtcctttct tctgcctaag
    25141 cttgccctat gcgcagggct tgaccacttt actttggttt cgctgaatta agcgaaaatt
    25201 aaaatagttc tcttgcagga gaaggacaat gtatatttat tggattttat taggtctggc
    25261 tattgctaca gaaattaccg gtacgctgtc aatgaaatgg gcgagcgtca gtgagggaaa
    25321 tggcggcttt attttaatgc tggtgatgat ttctctgtcg tatatatttc tctctttcgc
    25381 cgttaaaaaa atcgccttag gcgtagctta tgcgctgtgg gaaggtatcg gtattttatt
    25441 tattaccttg tttagcgttt tgttattcga cgaaagttta tcgctgatga aaattgccgg
    25501 gttaaccacc ctggtcgccg ggatagtgtt gatcaaatca ggtacccgta aagcgcgtaa
    25561 acctgaactg gaggtgaacc atggcgcagt ttgaatgggt tcacgccgcc tggctggcat
    25621 tggcaatcgt gctggaaatc gttgctaacg tctttttgaa attttctgac ggctttcgtc
    25681 gcaaaatatt tggcttgctc tccctggcgg cggtgctggc ggcctttagt gcgctttctc
    25741 aggccgttaa agggatcgac ttgtctgtcg tttatgcatt gtggggcggg tttggtattg
    25801 ccgccacgtt agccgcaggt tggatcttgt ttggtcaacg gttaaatcgt aaaggctgga
    25861 ttggcctggt cttgctgttg gctggaatga tcatggtgaa acttgcctga tgaagacgct
    25921 gcccgcgctg tcgggcagcg tttgaacatt atttttgcga cagttgatcc agcttgtcgc
    25981 gaaaaccggt aacagaaatg gcccggttat cggcgcgcca gcgatctttc gcggcaggag
    26041 ccgaactttg caccccaatt aattgccagc cgtcatcggt atgcaacatc agaggcgaac
    26101 cgctgtcacc cggcaaggta tcgcactgat gtgacatcac cgacgtttgc gcccagccag
    26161 tcacttcaca gttttgatga ctgtataacg tatcgagatg atcttcaggg tatcccgcct
    26221 gagtcacttt acgaccagcc gattttaatg cggcagtaag cgcggcttta tctccctcaa
    26281 ataacggcaa cggtgtaatg ccagaagggg gattacgtag cacaatcaat ccgaagtccc
    26341 acggtgcagc tgcgggagga acaatccaac catccccatc tgcttttaac cgctttccca
    26401 gtgtcggatc aacgcggcct tctatgtcgt ggatctcata gcgccaaaga cctttatttg
    26461 acacaaaacg cagcgctacc gccttatccg ctttaccctt aggcggcgtc aataaacagt
    26521 gtcctgccgt taatgccaga ttgggtgcaa tcagcgtcgc cgtacataaa ttgccgctgg
    26581 ccgtttccag ttgcccaacc gcatcccacg gtgattgggt cgtgtcattc actggcacac
    26641 gatcatcatg accaaaaaac agggtgctga cctcatcgtt tgccgatttg gcaacgtctg
    26701 gtttatctgc aaacacaaaa gcagacgtca aactaattgc acccaacact acagcaatgg
    26761 ttgtacgcat atcacactct ggtgggtaat tatgattatt aaaagcaaac cctcataaat
    26821 actatagacg ggacgggatg aaagtgggag taaaatcaga taactacagt caggaaagat
    26881 aaaaacgggc ggcaatcagt gcgcataaaa taaggatgat aagaatgaac tcaaagcgat
    26941 aacggcccac catactaccc tccgctaaag gcggtgctaa acgttacccg cttagcaccg
    27001 taattttcag gcgcgagggg gcgcgccagc attactgttg aaaacttacg ctgcgggttg
    27061 tgcagcaggt tttgctggtt gctgatggcc gtgtttcccg gcgtgtttct tcgctgcctg
    27121 cgctttttgt tcaggggctt tctgttcagc tttcgtattt ttatgatgct ttttagccgc
    27181 ctgcgctttc tgggcaggtg ctgctttatg ctgtttttta tgatgtgtag ttttcgctgg
    27241 cgctgctttg gtggtcgtcg cagtcggagc aggtgtggtc gcagtctctg cagcaaaggc
    27301 ggcagaagac agacccatag cagcggcaac aaccagagct aatacttttt tcattgtcat
    27361 accctcaatt tgttttttca tttaacccca ctgcggggcc gttgaaataa ctatatccct
    27421 gggaatggca gacttccgtg agtggttggt ttcagcgtgt aacgatatgt acaaacgctg
    27481 aataaattac agcgttgata ataacgtatt gtgacttaag gaaaatttag ctaccaataa
    27541 tagtagtctt gatcgggtta actattatac tacttacaaa gcggtagaat aactgcgcat
    27601 cagtaataaa tgacacacag caaaatgaat ccgtttattt gggtacttat aatcctgatg
    27661 acgctagatg cgctgcggga attggctggc gcttcgtcta ttttaggatg gctattaacg
    27721 ttggtttgag caggcaataa gtccggacgg gtatttaccg cagtccggac ttatttttca
    27781 ggcgtgcaga cgacgatgca aacgcgtccc gaccagcaga gcaatgacca gcatcagagc
    27841 aataaatgcg ccgacgccgt tccagccata gttatgccag aaaacaccac ccagcgtccc
    27901 ggcaatactc gaccccagat agtaactgaa cagatacagc gaggaggcct ggcctttagc
    27961 gcgttttgcg cgggggccga tccagctgct ggctactgag tgggctgcga agaatccggc
    28021 tgagaagagt aacattccgg caaagatcag ccacagcgag ctaaataagg tcatcagtaa
    28081 accaaacggc ataactcccg tcgaaaacaa catcaccgga ccgcgcccat agcgggtggt
    28141 catggttccg gctttgggtg agctccatgt accggtcaaa taagccagcg ataataagcc
    28201 aaccacggcc tgactgacat gctaggggga aagcatcaac cgatagccga tgtaattaaa
    28261 cagcgtgacg aacgacccca tcagcaaaaa gccttctgcg aacaataacg gtaatccccg
    28321 gtcacgccag tgcagacgaa agttgataaa taacgtctta gggcgcagcg aagtcgggca
    28381 aaaatggcgt gattcaggga ggattttcca gaacatcaac gccgaggcca gcgcgaaaca
    28441 accgattgcc gccagagcaa ttcgccagtt gaaaaagtcc gtgaagacac cactaattaa
    28501 gcgtccgctc atgccgccaa ttgagttgcc gctgatatac aaccccatcg aaaaggccac
    28561 gaaactgggg tggatttcct cgctaagata agtcatgcca acagctgcca cgccacttaa
    28621 cgaaagccca atcaaggcgc gcataatcaa aatgccgtgc cagctggtca tcattgtcga
    28681 aagtaacgta caaatggagg ccaacagtag cgccgtgacc atcactggtt tgcgaccaat
    28741 ggcatcggat agcgggccag taaatagcaa accgatagcc aacatcgccg tggaaatgga
    28801 cagtgaaata ctactgttcg cgggcgttaa gccaaactcc tgcgaaagca ccggaaggat
    28861 aggctgcaca caatagagaa gtgcaaaggt tgccagtccg gcagagaaca gcgccagggt
    28921 gacgcgcata aattgcggcg taccgcgttt aataaattga tttggctgag aaatgctttg
    28981 cttgtcagtg tcgcttgccg gagcgccatc aacagttgta gtacggctca cttgaaatcc
    29041 ttgctaaata tgcctgtgga tcaggcttat acatagggta ggaaaatcga attgttctgt
    29101 ctaatatatt aataatctca aataagatgt tttaaatatg aatattgaac ttcgtcatct
    29161 gcgttacttt gttgctgttg cggaagagct gcattttggg cgcgcggctg cccgcctgaa
    29221 tatttcgcaa ccgccgctaa gtcagcagat tcaggcactg gagcaacaaa tcggtgcccg
    29281 actgctggca cgaactaatc gcagtgtatt gctgacggca gcaggaaaac agtttcttgc
    29341 agatagtcgg caaatcctgt ctatggtgga tgacgctgcc gctcgcgctg aaagactgca
    29401 tcagggtgaa gcgggggagt tgcgtattgg ttttacttcg tccgctcctt ttattcgggc
    29461 ggtgtccgat acgttatcgc tgtttcgccg tgattatcct gatgtccatt tacaaacccg
    29521 cgaaatgaac actcgcgagc aaatcgctcc gctcattgaa ggaacgctgg atatgggatt
    29581 gctgcgtaac acagcgttac cggagacgct tgaacacgca gtcatcgtcc atgaaccgct
    29641 tatggcgatg atcccgtacg atcatcccct ggcaaataat ccgagtgtaa cactggctga
    29701 actggctgaa ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg ggactgggct
    29761 gtatgacgat attctcggac tgatgcgacg ttaccatttg acgcccgtca tcactcagga
    29821 ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg tttcaatttt
    29881 gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga ttgctgaaga
    29941 ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat catgaacaaa gtccagctgc
    30001 gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga gggaaatttc agcgaaaaag
    30061 cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac gtattgaagt
    30121 gcttcaccat agcctacaga ttatttcgga gcgcgaaaat atagggagta tgcggtggtt
    30181 gctgaaaacc agcctgggca cattgatcaa ataaagcaga ccaacgcggg cgcggtttat
    30241 cgcctgattg atcagcttgg tccagtctcg cgtatcgatc tttcccgtct ggcgcagctg
    30301 gctcctgcca gtatcactaa aattgtccgt gagatgctcg aagcacacct ggtgcgagag
    30361 ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg tggggctggt ggttgaaact
    30421 gaagcctggc actatctttc tctgcgcatt agtcgcgggg agattttcct tgctctgcgc
    30481 gatctgagca gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa agatgacttg
    30541 ccattgctgg atcgtatcat ttcccatatc gatcagtttt ttatccgcca ccagaaaaaa
    30601 cttgagcgtc taacttcgat tgccataacc ctgccgggaa ttattgatac ggaaaatggt
    30661 attgtacatc gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg cgaggcgctg
    30721 gagcagcata ccggcgtacc ggtttatatt cagcatgata tcagcgcatg gacgatggca
    30781 gaggccttgt ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt tatcgatcac
    30841 aacgtggggg tgggcgtcat taccgatggt catctgctac acgcaggcag cagtagtctc
    30901 gtggaaatag gccacacaca ggtcgaccca tatggtaaac gctgttattg cgggaaccac
    30961 ggctgcctcg aaaccatcgc cagtgtggac agtattcttg agctggcaca gctgcgtctc
    31021 aatcaatcca tgagctcgat gttacatggg caaccgttaa ccgtggactc attgtgtcag
    31081 gcggcattgc gcggcgatct actggcaaaa gacatcatta tcggggtggg cgcgcatgta
    31141 gggcgcattc ttgccatcat ggtgaattta tttaacccac aaaaaatact gattggctca
    31201 ccgttaagta aagcggcaga tatcctcttc ccggtcatct cagacagcat ccgtcagcag
    31261 gcccttcctg cgtatagtca gcacattagc gttgagagta ctcagttttc taaccagggc
    31321 acgatggcag gcgctgcact ggtaaaagac gcgatgtata acggttcttt gttgattcgt
    31381 ctgttgcagg gttaacattt tttaactgtt ctaccaaaat ttgcgctatc tcaatttggg
    31441 ccaggaaagc ataacttaga ctttcaaggt taattatttt cctagtttat atttgtgaag
    31501 cataacggtg gagttagtga tgctgaagcg tttctttatt accggtacag acacttctgt
    31561 agggaaaacg gtggtttccc gcgcattgct acaagcgtta gcctcccagg gaaaaagggt
    31621 tgcgggatat aaacccgtag cgaaggggag caaagagaca cccgaagggc tgcgtaataa
    31681 agatgccttg gtgttgcaga gtgtttcaac catcgaactg ccttatgaag cagttaatcc
    31741 tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca attacaccct
    31801 catttcaaac ggcctggcaa acctgaccga taaagtcgat catgtcgtgg tagaagggac
    31861 tggcggctgg cgcagtctga tgaatgattt gcgtccactc tctgaatggg tagtgcagga
    31921 acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc atgcactgct
    31981 aacagctcag gcgatcgcca acgacgggct gccgctcatt ggctgggtgg ctaaccgaat
    32041 caacccagga ctggcgcatt atgcggaaat cattgatgtg ctgggtaaaa aacttccggc
    32101 accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac tggggcaata
    32161 catccgctta gctatgctgc gcagtgtgct ggcggtagat agagtcacgg tgtaacgtcc
    32221 gcgaaattac cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc tgatactccc
    32281 cggtcatttc acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt gccgccaaca
    32341 gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca gggaaccata
    32401 atcccaggct acgaccatac aacatgccaa tggcaagacc gataaatagc gtcggtgtaa
    32461 agacaccacc gggtgcgccg gaaccgctac tcgccagcac ggcacacagt ttacaaagga
    32521 agatcccggc aatgatcatt aacagtggtg gggcggttaa aaaggattgt acggtgctat
    32581 agccgttgcc ccacactgca ggtgtgaaca gggaaagcag acccacgatc aacccgccta
    32641 gtgccagttg ccagggcggc gcaagtttga gactcacaaa tccacgatga caggcgttca
    32701 ttaatgttaa caacagtggt ccgcacagac ctgccagcac acctgtactg ataatcagcg
    32761 catagtcacg agcctgaacc gtcactgaga gttgtacgtt gtagagtaac gcgtcgctat
    32821 gattaatcag attgctaacc agccatgcca cgatggcgga aataatcacc gggccgagag
    32881 aggccaacat catagtgcca aacagcactt cggctataaa taaactgcca gcaagcgggg
    32941 cacgataggc cgcagccatt cccgccgcgg ccccacaggc gatccataat ttccattcct
    33001 ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa agaatcatcg
    33061 caccttcgcg accaattgca ctgccgctgg ttactaccag cagcgaggca agcgatttaa
    33121 ccaggcttgc tgcgtaatcg aactgtccat cggtttgcaa cgcttccatg taatcggtcg
    33181 gcgcatgagg gcgttgttgg gtaaatttct gccagcccat cagcaacaaa cccgccgcca
    33241 gtccgccgag cgccggagtt agcagccgtc gccaggagga aaggtttgtc gctgcattga
    33301 ccagactgcc ggagtcatta ttgaggaaca accactccag tagcagcatc gcatgacgaa
    33361 acccggcaac ggcaaagaca gcgagaatac cgacgactgt tgcgataagc agacggcgga
    33421 acatagtgcg cagatccgga tagatgtgta gatgatgcat gagcagaaat gggcaatgaa
    33481 acacgggcga ctattttgcg gcaaaagagg ccaaccgcaa aagcagtgcg tgctttaaga
    33541 gataaaaaaa cgtgacattg taacgtatta ttgccgaatc gcgcgtaatc gtcaccatcc
    33601 ggcaatatta cggtgatcct atcgaaagag tggtttaacc gcgacaggaa tcaacagttg
    33661 tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt agaaagggtg
    33721 ttctgctttt tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa ggtgccatgc
    33781 cagcaattct tcgcattctg tctggcgacc attctccgcc aaccacgcag ccatcaacag
    33841 cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa tgcctttctc
    33901 gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt cgcgatccag
    33961 ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca aacgctgcca
    34021 ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg gcgctaatga
    34081 cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg caaccagagg
    34141 tgcggcttcc gcactctctg gagcgtaata aaacagcgcg cccaacacgc gcgccgcgac
    34201 agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat gtgcgggttg
    34261 ccccgcacca atttgttaac ctgcaatagc cattcctact gtcatgtgca ggccataaaa
    34321 gagcacacgc ccgatcatct caccaacaag tatgaggatt agccccagaa caagtccggc
    34381 aacgtgaggt tcacggcgac gaatgagcgg acaaagccag catccaagac ctgcgcataa
    34441 caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc tggcttgttg
    34501 cacggatgag tgaatagacg ccagagacaa accttgcagc acaatcacct cggcacaagc
    34561 gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa cgcgggctgc
    34621 ccgcagaatt gcggcagcaa gtattggacc gctcaacaat accgtcagga agaaagccag
    34681 cgtagtgtaa ccgttatgcc aggttggcac ggtgtcgatt tgatacacgc aggtcatcat
    34741 ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc ccaacgcttg
    34801 cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa aaaatatgga
    34861 accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg agttaaacgc
    34921 tcgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca tcaacagcca
    34981 gaggaaaaac atgccacgaa caatgtgctg acggtctgcg tcatttttgg cggcgaacca
    35041 gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg taaagataac
    35101 cagtggccat tcatgccatc catttcccat tttacacttc ctccggatta gccagataac
    35161 cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata ttgggttttg
    35221 tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc tggcgcagct
    35281 cttcaattgg accgaactca agtgcacgca gcgggcagga ttcgacgcat atgggttgtt
    35341 tcccctcggc gacgcgcgaa taacagccat cgcacttcgt catgtgccct ttttcagcat
    35401 tgtactgtgg tgcgccgtac ggacaagcca tgtggcagta gcggcagccg atacaaacat
    35461 cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgccgctg gggcagactt
    35521 ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa gcgaacacgt
    35581 tctggtgcca gatgccatta tcttcctgcc agtcgccgcc agcatattca taaatacggc
    35641 ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg caggttttac
    35701 agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg ggctactcct
    35761 taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc cagcggtgaa
    35821 gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc accaaacata
    35881 tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat acgcggagtc
    35941 acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt atcgccatga
    36001 cggataccgc gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg gcaggcctgc
    36061 tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttggtttg gcgacttcac
    36121 tcagcatcca gtagatgggt ttgcgttcaa attttgctga ggttgcgggt tgggcgagga
    36181 tcacatagcc catattccct gcagattcat gagagataag gtcttcttgc tctgttggca
    36241 tcaggtcagg caacaggata tcgcaatact tagccgaggc cgtcatgaag tggtcaatgc
    36301 caacaatcat ctcgcacttg ctgtcatcct gaagcacctc atgggtgtga ttgatgtcgc
    36361 catgttgatt gatcaatgtg ttactggcgt agcaccataa aaacttgatg gggacatcca
    36421 gtttttcttt tccacgaaca ccatcacggg tcgcggtcat tttcgtacca tggtcgatgg
    36481 catctgtcca tgtaaaaacg gaaatctgcg ttttaacagg attctcgagc atcgggaacc
    36541 attctacccc cagatcccag ctaccttcgc gtacacctga gttgccgccg tttatgccga
    36601 cgttaccggt aagaacggaa agcatggcaa tagcgcggga cgtttgttcg ccgttggagt
    36661 gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt tgctgaaccg atctctcgtg
    36721 ccaactggat aattttttct gccgggatgc tggtgatttt tgctgcccat tccggcgttt
    36781 tagctatgcc gtcaggccct tcgcccagaa tataggcttt ataatgcgcg ttacgtggtg
    36841 cgttggcggg cagcgttttt tcatcgtaac caacacaata tttgtcgaga aatggctgat
    36901 cgaccatgtt ttcagtaatc agtacccagg caatcgcaca ggccagtgcg ccatcggtgc
    36961 cagggcgaat gggcagccat tcatcttcac gcccggcagc tgtgtcgtta tagcgtggat
    37021 cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg ctcgacgtag taagtgacac
    37081 caccgccgct catccgcgtt tctgccgggt tatttccgaa cataacgacc agtttcgtat
    37141 tggcgatatc atccgggctg ttgccatcat tggcaccgaa catataactc attgcggcac
    37201 tgatctgtgc ggtactgtag ctgccatagc gactgagaaa acccccgcaa gagttcatca
    37261 gacggtacgg gacgttcgag ttggtgatat ttccgccatc tacgcctgtt ccgtacagga
    37321 catgtacagc ctcattgccg taatctttca ggatccgccg aagattatca ctgatggtat
    37381 ccagggcttc gtcccaactt atccgttcaa atttaccttc accgcgctcg ccgacgcgct
    37441 tcatgggata tttcaacctg tcaggatgat tcatccgtcg gcggatagag cgcccgcgta
    37501 aacatgcccg aacctgatga ttaccgtaaa cgtcgtcacc tgtcgtatca gactccaccc
    37561 agtacacggt gtcatctttc acatgcaaac gtagcagaca gcggctcccg cagttaacgg
    37621 tgcaggaact ccagaccgtt ttctcttcta ccggagcctc tgccgcccgg accatttggg
    37681 aaaatggcag agtgaaagca ctgcttgcca gcgcgagact gccaagtgcg gaggttttca
    37741 tcagacttct atggctgatt tcagccttca tgagcgcctc tgtggtatgg attttcatca
    37801 ttactcactt attgcttttc aaacaaaatg tcatgccaga atttatggtt gtagtgggtt
    37861 atattttttc gatctcgacc agattagtgt gctgcgggtt tcccttcgcc agtggtgaag
    37921 ggcgcagagt ggttagcgta ttcacacatc cgccatggtc gattttatcg ccagacatat
    37981 tgggctcgtg ccaggctccc tggcccatag cgctaactcc agggagaatg cgtggtgtta
    38041 ctttggctgg tagccgaact tcgccacgat ggttaaacac ccgcaccata tcgccgttgg
    38101 caatcccacg tttctgtgca tctatagggt tgatccacac ctcctgacgg caggctgcct
    38161 tcaggacatc aatgttgccg taggtcgagt gagtacggga tttgtaatgg aaaccaaaca
    38221 gttgcagtgg gaaggttcta cgttcagggg agtcccagcc ttcaaaggtt gaggcataaa
    38281 ctggcagtgg gcttatcact tcatcttttt ccagttccca ggtacgggca atttccgcca
    38341 gcttgctgga ataaatttca atcttaccgg aaggcgtttt aagtggattt gcctcggggt
    38401 cgtcacgaaa tgctttgtag gcgacaaaat ggccattggg atctttacgc ttatagatac
    38461 ccattttttt cagttcgtcg taagacggta acgccggatc tttggcaagc attttggcgt
    38521 acagatgttg taaccattgt tcctgcgtgc gaccttctgt gaacttttga tagacgtcag
    38581 gtccaagacg tttcgcgact tcactcagga tccagtaaat cggtttacgt tcgaattttt
    38641 cgctggtgac tggctggagg aaaatgagat atcccatgtt accggcgtag tcgttaggaa
    38701 taatatcttc ctgctcaacg gtcatcaggt ctgccagcag aatgtcggca tattttgccg
    38761 atgaggtcat aaagttttcg atgaccacaa tcatttcgca tttcgattcg tcctgcagaa
    38821 tttcatgcgt tttgttgatg tcagaatgct gattaacgag ggtatttccc gcgtagttcc
    38881 agatggactc cactgtcaga cggatggacg gacttgaagt agttgactca acgctactta
    38941 aggtcctgat atgactgaca ttaaccatga cgatactgca tatccatcca gttctttctc
    39001 tgggtctccc tatctgtatc agcaagtcta cacctcaagt cctgacacaa ggctgacaca
    39061 aatcaaggac cttgctacgc cacagctagt ccggagaacc aacgggcgtt acactgtccg
    39121 cttcagactt aaaggagcta ccagcccgtt tctatcagtc tcaacccgca gcacagaccg
    39181 gaggaccgct acaatgcgtc agagagagct cgcagccaca gctaaggcat tcctactgga
    39241 caatcctgaa gtcacactac aggagcttac agcgcacata cggtccatgg ctgagcagtt
    39301 cctgactgat gccagtgatg actactggaa tggcgtagag gtggcgacac tggttgacga
    39361 gaagtccaac ctcagggaac ttgcagcgac aagagcgttg agtgtggacc agcagagggg
    39421 cattaagtta gcgctggaag tcctgaccgc agcacagcag agagtcgaca ctggtgatac
    39481 ctctggtctg attaagctga ttgatggtgg cgtaagccag cttactgatg ataataacca
    39541 tactgattac tcaactatag gcgactctac gtcgattctg aagaatgagc agggggatag
    39601 accagcagtt ttcacacagg aacgtcagtt actcactcca gagtcacgag aatcatctgt
    39661 actttttcag gaattggtga catcactgct ggctgagaag gtccagacac tgaaacaatc
    39721 cagctttaag gacttgtcag cgtccc
//
LOCUS       DAJTRB010000029        37627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTRB010000029 DAJTRB010000000
VERSION     DAJTRB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.029"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..175)
                     /locus_tag="OO330_001614"
     CDS             complement(<1..175)
                     /locus_tag="OO330_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5882521.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO330_001615"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO330_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5882522.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO330_001616"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO330_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5882523.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO330_001617"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO330_001617"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5882524.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO330_001618"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO330_001618"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5882525.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO330_001619"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO330_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5882526.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OO330_001620"
     CDS             complement(5179..5421)
                     /locus_tag="OO330_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5882527.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO330_001621"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO330_001621"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5882528.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO330_001622"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO330_001622"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5882529.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OO330_001623"
     CDS             complement(8338..9312)
                     /locus_tag="OO330_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5882530.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OO330_001624"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OO330_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5882531.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OO330_001625"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OO330_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5882532.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OO330_001626"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OO330_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5882533.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OO330_001627"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OO330_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5882534.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OO330_001628"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OO330_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5882535.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OO330_001629"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OO330_001629"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5882536.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OO330_001630"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OO330_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5882537.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKELVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OO330_001631"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OO330_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5882538.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO330_001632"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO330_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5882539.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OO330_001633"
     CDS             23179..23382
                     /locus_tag="OO330_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5882540.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OO330_001634"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OO330_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5882541.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVHPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OO330_001635"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OO330_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5882542.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OO330_001636"
     CDS             26387..26659
                     /locus_tag="OO330_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5882543.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO330_001637"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO330_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5882544.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO330_001638"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO330_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5882545.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO330_001639"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO330_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5882546.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO330_001640"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO330_001640"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5882547.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO330_001641"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OO330_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="OO330_001642"
     CDS             complement(31906..32304)
                     /locus_tag="OO330_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5882548.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="OO330_001643"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="OO330_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5882549.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQNDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33844..34272
                     /gene="rplM"
                     /locus_tag="OO330_001644"
     CDS             33844..34272
                     /gene="rplM"
                     /locus_tag="OO330_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5882550.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34288..34680
                     /gene="rpsI"
                     /locus_tag="OO330_001645"
     CDS             34288..34680
                     /gene="rpsI"
                     /locus_tag="OO330_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5882551.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35075..35713
                     /gene="sspA"
                     /locus_tag="OO330_001646"
     CDS             35075..35713
                     /gene="sspA"
                     /locus_tag="OO330_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5882552.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35719..36216
                     /gene="sspB"
                     /locus_tag="OO330_001647"
     CDS             35719..36216
                     /gene="sspB"
                     /locus_tag="OO330_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5882553.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="OO330_001648"
     CDS             complement(36259..37626)
                     /gene="dcuC"
                     /locus_tag="OO330_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5882554.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9113 a         9416 c         9900 g         9198 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaagcaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gagttggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcatccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaatt ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acggcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agaatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttcccaaaac
    33721 tttttgtgtg ctggcatagg ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa
    33781 cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta actatttatt gggtaagctt
    33841 ttaatgaaaa cttttacagc taaaccagaa accgtaaaac gcgactggta tgttgttgac
    33901 gcgaccggta aaactctggg ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag
    33961 cacaaagcgg aatatactcc gcacgtagat accggtgatt acatcatcgt tctgaacgct
    34021 gacaaagttg ctgtaaccgg caacaagcgt actgacaaag tgtactatca ccacaccggc
    34081 cacatcggtg gtatcaaaca agcgaccttt gaagagatga ttgctcgccg tcctgagcgt
    34141 gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc
    34201 cgtaaactga aagtttacgc gggtaacgag cacaaccacg cggcacagca accgcaagtt
    34261 cttgacatct aatcgggatt ataggcaatg gctgaaaatc aatactacgg cactggtcgc
    34321 cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca acggtaaaat cgtaatcaac
    34381 caacgttctc tggaacagta cttcggtcgt gaaactgccc gcatggtagt tcgtcagccg
    34441 ctggaactgg tcgacatggt tgagaaactg gacctgtaca tcaccgttaa aggtggtggt
    34501 atctctggtc aggctggtgc gatccgtcac ggtatcaccc gcgctctgat ggaatacgac
    34561 gagtccctgc gttctgaact gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt
    34621 gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa
    34681 ttggcttctg ctacggcaga aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta
    34741 tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct ttttgctgcc gtctgaaaga
    34801 caggtaaaac aaaaaccaca tcgccaataa gggtctaagt caactatttc agactaaagc
    34861 gcatctcttt ttccccattt ccggcatcca ctcaccacaa aggtcgcaaa atctggtaaa
    34921 ctatcatcca attttctgcc caaatgtcgg gtattgctca ttttttgttt gattttcgaa
    34981 caaagagagt cgttccttat tgggtaacac aacttctgac tggccacctg gtggctggta
    35041 gcagtaaaaa ttctgactat acctggaggt tttcatggct gtcgctgcca acaaacgttc
    35101 ggtaatgacg ctgttttccg gtcctactga catctatagc catcaggtcc gcattgtgct
    35161 ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa aaggacaatc cgcctcagga
    35221 tctgattgac ctcaacccga atcagagcgt tccgaccctg gtggatcgtg agctgaccct
    35281 gtgggaatct cgcatcatta tggaatatct ggatgagcgt ttcccgcatc cgccactgat
    35341 gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga
    35401 ctggtacacg ctgatgaaca ctatcatcaa cggttcagct tctgaagcag atgccgcacg
    35461 taagcaactg cgcgaagaac tgctggcgat tgcgccggtc ttcggtcaga agccgtactt
    35521 cctgagcgat gagttcagcc tggtcgattg ctaccttgct ccgctgctgt ggcgtctgcc
    35581 gcaactgggc atcgagttca gcggcccggg tgcgaaagag ctgaaaggct atatgacccg
    35641 cgtctttgag cgtgactctt tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct
    35701 gggccggagt taatctgtat ggatttgtca cagctaacac cacgtcgtcc ctatctgctg
    35761 cgtgcattct atgagtggtt gctggataac cagctcacgc cgcacctggt ggtggatgtg
    35821 acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac
    35881 attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg
    35941 cgctttggtg gcattccgcg tcaggtttct gtgccgctgg ctgccgtgct ggctatctac
    36001 gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag ctgcctacga tgaagatacc
    36061 agcatcatga atgatgaaga ggcatcggca gacaacgaaa ccgttatgtc ggttattgat
    36121 ggcgacaagc cagatcacga tgatgacact catcctgacg atgaacctcc gcagccacca
    36181 cgcggtggtc gaccggcatt acgcgttgtg aagtaataca aaacaggccc aggcggcctg
    36241 ttttgtcttt ttaatgactt aataaaatag cgtgattgtg gcaatcatat tcaccacgaa
    36301 accgactgcc atggggatcg ctgtccgctt caccaccgca aaaggcgaaa cgcctgcaat
    36361 tcctgacgta acgaccacca ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc
    36421 aaaatgcatc ggcataatca ttacaaccgc tggtacatgt agtccggctg cgatattcgg
    36481 aataagactg gcaaatgaca taaacggcgc attgccagag cccatcacaa tggcacaaat
    36541 ggcaatgact agcgccataa taatcatcac gccaataccg cccagaccag aatgctccgc
    36601 acccctgata accgcatcaa cagtgccaat cgtcgttaag cctttcgcaa atatttcacc
    36661 cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc atgccgtcaa aaaacgcctg
    36721 cacatcgtcc attgtttcgc gcaagttatg cttgcggaag aactcaacaa acatcgtcac
    36781 cgtcaaactt agtaacatca ccaccaccag atgcagttct gacttcatca gcccgacgtg
    36841 ggcgaggaac agcgagccga gcatcaggat taacggcatc acaggtaaaa tggcgtaata
    36901 gagcggcggg acattatcga gagttttttg ctctgcctgt tcgtgattga tatttttatc
    36961 ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc gccaccgaga taatgacgca
    37021 agaggcgacc ggaagctggt agtggaagaa gtatgtggca attttcattc ccgcgacctg
    37081 ggctgcaaaa atggagttcg tttccagaat cccccactca atggacatcg tggttgcgat
    37141 aaccgccacc gcagagaggc gacttactcc cagactcacc agcgtcggaa ataaggtgac
    37201 catcagcaac atacccagac cggaggcgct ggtaataaac tgcgccatga tttggccgat
    37261 gacgtaagtt gccgacagaa taatatacgg cgagcgaatg agttttaacg ggcggcttag
    37321 caagctcacc atcgcgcaac tggctcctat gcgctccatg tagcgggcat aaccgcccac
    37381 cgccataatc gacagcccca atccggcaat gcggttggac agcatgcgca ggatttcatt
    37441 ataaatgtcg acgatcaagt agccgctgct tttggcggga tcgagtacac caccgaaccc
    37501 taaccagact ccgcacatca tcaggaagac acctgcggca gccagcacca cctgaggttt
    37561 gtagtttttc aggatcaaat agcccatcgt aattaatacg atgacagata taattatgcc
    37621 gaacatt
//
LOCUS       DAJTRB010000030        36638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTRB010000030 DAJTRB010000000
VERSION     DAJTRB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.030"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(156..1391)
                     /locus_tag="OO330_001649"
     CDS             complement(156..1391)
                     /locus_tag="OO330_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5882555.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO330_001650"
     tRNA            complement(1538..1614)
                     /locus_tag="OO330_001650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OO330_001651"
     tRNA            complement(1657..1743)
                     /locus_tag="OO330_001651"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OO330_001652"
     tRNA            complement(1764..1839)
                     /locus_tag="OO330_001652"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OO330_001653"
     tRNA            complement(1898..1974)
                     /locus_tag="OO330_001653"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OO330_001654"
     CDS             complement(2077..3462)
                     /locus_tag="OO330_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5882556.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO330_001655"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO330_001655"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5882557.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO330_001656"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO330_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5882558.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO330_001657"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO330_001657"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5882559.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO330_001658"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO330_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5882560.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO330_001659"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO330_001659"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5882561.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO330_001660"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO330_001660"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5882562.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO330_001661"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO330_001661"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5882563.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO330_001662"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO330_001662"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5882564.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO330_001663"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO330_001663"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5882565.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO330_001664"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO330_001664"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5882566.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO330_001665"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO330_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5882567.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO330_001666"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO330_001666"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5882568.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO330_001667"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO330_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5882569.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO330_001668"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO330_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5882570.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO330_001669"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO330_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5882571.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OO330_001670"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OO330_001670"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5882572.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OO330_001671"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OO330_001671"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5882573.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO330_001672"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO330_001672"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5882574.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OO330_001673"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OO330_001673"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5882575.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO330_001674"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO330_001674"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5882576.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OO330_001675"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OO330_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5882577.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO330_001676"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO330_001676"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5882578.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO330_001677"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO330_001677"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5882579.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO330_001678"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO330_001678"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5882580.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO330_001679"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO330_001679"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5882581.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OO330_001680"
     CDS             complement(31267..32913)
                     /locus_tag="OO330_001680"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5882582.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OO330_001681"
     CDS             complement(32916..32966)
                     /locus_tag="OO330_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882583.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO330_001682"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO330_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5882584.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="OO330_001683"
     CDS             33502..35022
                     /locus_tag="OO330_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR5882585.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OO330_001684"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OO330_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5882586.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="OO330_001685"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="OO330_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5882587.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="OO330_001686"
     tRNA            complement(36439..36514)
                     /locus_tag="OO330_001686"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
     gene            complement(36523..36599)
                     /locus_tag="OO330_001687"
     tRNA            complement(36523..36599)
                     /locus_tag="OO330_001687"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36563..36565),aa:Asp,seq:gtc)
BASE COUNT         8944 a        10035 c         9232 g         8427 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcataacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg gaatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag ggtggcggaa cggacgggac
    36541 tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac taccgctcca
    36601 ccgaattctt ttacaaccac cggttttatg accggctt
//
LOCUS       DAJTRB010000031        36604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTRB010000031 DAJTRB010000000
VERSION     DAJTRB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.031"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(64..822)
                     /gene="deoR"
                     /locus_tag="OO330_001688"
     CDS             complement(64..822)
                     /gene="deoR"
                     /locus_tag="OO330_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5882588.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(880..1476)
                     /gene="ybjG"
                     /locus_tag="OO330_001689"
     CDS             complement(880..1476)
                     /gene="ybjG"
                     /locus_tag="OO330_001689"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5882589.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSMFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1761..2987
                     /gene="mdfA"
                     /locus_tag="OO330_001690"
     CDS             1761..2987
                     /gene="mdfA"
                     /locus_tag="OO330_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5882590.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3028..3312)
                     /locus_tag="OO330_001691"
     CDS             complement(3028..3312)
                     /locus_tag="OO330_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882591.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3398..4213)
                     /gene="ybjI"
                     /locus_tag="OO330_001692"
     CDS             complement(3398..4213)
                     /gene="ybjI"
                     /locus_tag="OO330_001692"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5882592.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4213..5421)
                     /locus_tag="OO330_001693"
     CDS             complement(4213..5421)
                     /locus_tag="OO330_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5882593.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5505..6041
                     /gene="rcdA"
                     /locus_tag="OO330_001694"
     CDS             5505..6041
                     /gene="rcdA"
                     /locus_tag="OO330_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5882594.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6216..7901)
                     /locus_tag="OO330_001695"
     CDS             complement(6216..7901)
                     /locus_tag="OO330_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5882595.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8171..8548
                     /locus_tag="OO330_001696"
     CDS             8171..8548
                     /locus_tag="OO330_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5882596.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8578..8835)
                     /gene="grxA"
                     /locus_tag="OO330_001697"
     CDS             complement(8578..8835)
                     /gene="grxA"
                     /locus_tag="OO330_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5882597.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8995..9282
                     /locus_tag="OO330_001698"
     CDS             8995..9282
                     /locus_tag="OO330_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5882598.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9266..9988
                     /gene="nfsA"
                     /locus_tag="OO330_001699"
     CDS             9266..9988
                     /gene="nfsA"
                     /locus_tag="OO330_001699"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5882599.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPGLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDFLHKQGWATR"
     gene            10049..10951
                     /gene="rimK"
                     /locus_tag="OO330_001700"
     CDS             10049..10951
                     /gene="rimK"
                     /locus_tag="OO330_001700"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5882600.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11039..11515
                     /locus_tag="OO330_001701"
     CDS             11039..11515
                     /locus_tag="OO330_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5882601.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11866..12978
                     /gene="potF"
                     /locus_tag="OO330_001702"
     CDS             11866..12978
                     /gene="potF"
                     /locus_tag="OO330_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5882602.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13073..14206
                     /gene="potG"
                     /locus_tag="OO330_001703"
     CDS             13073..14206
                     /gene="potG"
                     /locus_tag="OO330_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5882603.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14216..15169
                     /gene="potH"
                     /locus_tag="OO330_001704"
     CDS             14216..15169
                     /gene="potH"
                     /locus_tag="OO330_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5882604.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15166..16011
                     /gene="potI"
                     /locus_tag="OO330_001705"
     CDS             15166..16011
                     /gene="potI"
                     /locus_tag="OO330_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5882605.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16071..16559
                     /locus_tag="OO330_001706"
     CDS             16071..16559
                     /locus_tag="OO330_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5882606.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16600..17727
                     /gene="rlmC"
                     /locus_tag="OO330_001707"
     CDS             16600..17727
                     /gene="rlmC"
                     /locus_tag="OO330_001707"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5882607.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17902..18633)
                     /gene="artJ"
                     /locus_tag="OO330_001708"
     CDS             complement(17902..18633)
                     /gene="artJ"
                     /locus_tag="OO330_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5882608.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18925..19593)
                     /gene="artM"
                     /locus_tag="OO330_001709"
     CDS             complement(18925..19593)
                     /gene="artM"
                     /locus_tag="OO330_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5882609.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19593..20309)
                     /gene="artQ"
                     /locus_tag="OO330_001710"
     CDS             complement(19593..20309)
                     /gene="artQ"
                     /locus_tag="OO330_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5882610.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20316..21047)
                     /gene="artJ"
                     /locus_tag="OO330_001711"
     CDS             complement(20316..21047)
                     /gene="artJ"
                     /locus_tag="OO330_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5882611.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21065..21793)
                     /gene="artP"
                     /locus_tag="OO330_001712"
     CDS             complement(21065..21793)
                     /gene="artP"
                     /locus_tag="OO330_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5882612.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22011..22526)
                     /locus_tag="OO330_001713"
     CDS             complement(22011..22526)
                     /locus_tag="OO330_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5882613.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23175..24944
                     /locus_tag="OO330_001714"
     CDS             23175..24944
                     /locus_tag="OO330_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5882614.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25155..25478
                     /locus_tag="OO330_001715"
     CDS             25155..25478
                     /locus_tag="OO330_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5882615.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25475..26305
                     /gene="amiD"
                     /locus_tag="OO330_001716"
     CDS             25475..26305
                     /gene="amiD"
                     /locus_tag="OO330_001716"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5882616.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26302..27315)
                     /locus_tag="OO330_001717"
     CDS             complement(26302..27315)
                     /locus_tag="OO330_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5882617.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27414..28844)
                     /locus_tag="OO330_001718"
     CDS             complement(27414..28844)
                     /locus_tag="OO330_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5882618.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28855..29856)
                     /gene="ltaE"
                     /locus_tag="OO330_001719"
     CDS             complement(28855..29856)
                     /gene="ltaE"
                     /locus_tag="OO330_001719"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5882619.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29893..31611)
                     /gene="poxB"
                     /locus_tag="OO330_001720"
     CDS             complement(29893..31611)
                     /gene="poxB"
                     /locus_tag="OO330_001720"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5882620.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31744..32712)
                     /gene="hcr"
                     /locus_tag="OO330_001721"
     CDS             complement(31744..32712)
                     /gene="hcr"
                     /locus_tag="OO330_001721"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5882621.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32724..34376)
                     /gene="hcp"
                     /locus_tag="OO330_001722"
                     /gene_synonym="priS"
     CDS             complement(32724..34376)
                     /gene="hcp"
                     /locus_tag="OO330_001722"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5882622.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34520..35419)
                     /gene="lysO"
                     /locus_tag="OO330_001723"
     CDS             complement(34520..35419)
                     /gene="lysO"
                     /locus_tag="OO330_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5882623.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(35876..36571)
                     /gene="aqpZ"
                     /locus_tag="OO330_001724"
     CDS             complement(35876..36571)
                     /gene="aqpZ"
                     /locus_tag="OO330_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5882624.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9155 a         9108 c         9372 g         8969 t
ORIGIN      
        1 gcagctggtt ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg
       61 tcattaatac atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg
      121 gcaacaatca ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac
      181 cttgccaaat ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca
      241 gtgttttacc ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc
      301 cgccgcggaa taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat
      361 gggtttgaaa atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg
      421 gggtttctct ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat
      481 ttcattatca atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt
      541 ctgatcgggt tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccaa
      601 gcgggatttt tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata
      661 gccgccgagc aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc
      721 cgaaacccca agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc
      781 ttgcagcagc tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta
      841 ttgaagcgag ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt
      901 gcggatcggt aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg
      961 ccccatcgct tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc
     1021 accgagcata tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac
     1081 ggccagcacc attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc
     1141 aaaggtgaaa atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag
     1201 gaagttatag ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt
     1261 ccaggacaca aacatgctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg
     1321 tgctgtaagc ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc
     1381 tttagcaata aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat
     1441 aagagagaat agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa
     1501 aagggagcca atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg
     1561 catcgtctta tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt
     1621 aaataaaact taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt
     1681 ggtttcagct acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt
     1741 attattggcg aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc
     1801 aggcgttact tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg
     1861 atatgatcca acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg
     1921 ttcctacttc gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc
     1981 cgctgtcgga tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg
     2041 tcacctgtct ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct
     2101 tgcagggcat aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct
     2161 tcgaagaggc ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc
     2221 cgctacttgg tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt
     2281 ttgttttgtt tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg
     2341 aaaccgccat gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc
     2401 tggtgctgaa gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc
     2461 tggcgtggat cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg
     2521 aatatggctt gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag
     2581 cgcgtctgac ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta
     2641 tgattggtct gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga
     2701 tgaccgccgg gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc
     2761 gattaaccct gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc
     2821 tgcaaatgct gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg
     2881 gcaacggact gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg
     2941 ttatcttttt aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat
     3001 tgatttgtgc gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat
     3061 aaccatccct tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg
     3121 agctggcgca ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg
     3181 aacgctgact gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac
     3241 actttgcgcc atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt
     3301 tttcataatc atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct
     3361 tcattcaggc attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt
     3421 gtttaagaac tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt
     3481 attttgccgc tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct
     3541 gacgcagcat ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta
     3601 ttccccataa tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca
     3661 gatcgatgct gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta
     3721 atgctttttg tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa
     3781 tatcttctaa gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca
     3841 ccgttttcat ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa
     3901 taatttccac ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg
     3961 acagctcgcc attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca
     4021 caaaggcaat ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc
     4081 cgctggcgac cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc
     4141 gctcacggtt ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta
     4201 atttaatgct catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac
     4261 ggctttcgcg acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg
     4321 taatccataa tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata
     4381 accggtcgtt gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc
     4441 agaaatggtc agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc
     4501 tgcgacccag gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc
     4561 actggcccga accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg
     4621 tccaacggtc atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa
     4681 accgtgacca tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc
     4741 catcgccagc actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc
     4801 tttttcgcca tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg
     4861 aatggcgata taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc
     4921 cggaacgcca aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct
     4981 ataaaaaccg tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc
     5041 ttcaacgttt atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac
     5101 ggcaaagagc aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa
     5161 tgcgcaggac atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc
     5221 cgagcagaga ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc
     5281 agcgatcgag acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat
     5341 taacaggcct ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt
     5401 acgtgaagaa tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc
     5461 aaggttgtgt acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg
     5521 atccgcaacg gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa
     5581 tacatgctgt tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga
     5641 cctactattt ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga
     5701 ttatgtcccg gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc
     5761 aggctatcac cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga
     5821 tgtaccagct ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact
     5881 ggatgcagcg cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc
     5941 ttgatgcgtt tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc
     6001 gcgaggagat tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac
     6061 aaaaaaccca tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac
     6121 atcaaagaaa agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg
     6181 tgcaaaaaaa tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg
     6241 acgatgattg tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag
     6301 cccagcgcag ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag
     6361 gtgcgtgcgc ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca
     6421 ccgaacaaga aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc
     6481 tgaccgccaa tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct
     6541 gccataaaca ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa
     6601 ccgaaggtcg ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg
     6661 gcagcgttcc ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc
     6721 agcccaataa caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag
     6781 ataaagccga tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa
     6841 acgtcacctt tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg
     6901 acgcggttaa ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca
     6961 gcgttatggt ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga
     7021 tcgaaaactt ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag
     7081 cctaccaacg ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga
     7141 attccgttac gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca
     7201 cgcagatttt tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc
     7261 accggtaaat aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc
     7321 tgggcgctgg tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc
     7381 aaactcacca gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc
     7441 agtgagagct gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga
     7501 accggtgtcg acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca
     7561 aacagcttac ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac
     7621 attaggtaat ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc
     7681 ccgacgcaga aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa
     7741 tgttgttggc ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc
     7801 gaaccaagtc gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga
     7861 atgtaattcc catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt
     7921 actagtaagc tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg
     7981 cgcagattca ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa
     8041 tcacattagg tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa
     8101 gcggctttat cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca
     8161 ggaggaacga gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt
     8221 cattgtggtg tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca
     8281 tcctgaaatc ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca
     8341 gcgtagagaa gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct
     8401 cattatgcgg ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact
     8461 aagcgcggtg ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt
     8521 taaaccacag cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca
     8581 ggcgtccaga ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg
     8641 atcgacaaaa atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc
     8701 tttagtgatc ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt
     8761 gctcaatttc tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa
     8821 aataacggtt tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa
     8881 tgtattcgct gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa
     8941 acacaataca gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc
     9001 gcgatcggta aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg
     9061 gcggtggcgc tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca
     9121 gaagtgcaga ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg
     9181 gttattcttc aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat
     9241 tcacgttcag aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg
     9301 ctccattcgc catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa
     9361 cagcgcccgt gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac
     9421 cgacaaagca ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca
     9481 agcggcggag ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga
     9541 tgctcagctc ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc
     9601 gcagaatgca ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct
     9661 gcgcaataat attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct
     9721 gtttgggctg tgccttggct ggcctgcgga taatccggat ctcaagccgg gtttaccggc
     9781 ctccattttg gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta
     9841 tgacgagcaa ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg
     9901 gagcgatcat atccgccgaa caatcattaa agaaagccgc ccatttattc tggatttttt
     9961 gcacaaacag ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt
    10021 ttgaccaggt ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac
    10081 gctctattcg tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat
    10141 tcttgatccg ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa
    10201 aggccgcaag ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt
    10261 ttatgggacg gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc
    10321 ggtcgccatt gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg
    10381 catcgacctg cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga
    10441 catggtcggt ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt
    10501 cgtgctggcg gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa
    10561 cgcgcatatt ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct
    10621 ggttgttggt gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg
    10681 ttccaatttg catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga
    10741 aatcgcgata aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg
    10801 tgctaatcgc gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga
    10861 aaaaaccacc ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac
    10921 gacagaatat tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg
    10981 cctgcgcttt ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat
    11041 gacatcgctg gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat
    11101 gagttttttt gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga
    11161 cggtgtcttt gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc
    11221 ggaagtccga ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag
    11281 ttcgctgacc gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt
    11341 ttgccagtct ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg
    11401 tcagagcgaa gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt
    11461 accgactgac gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt
    11521 cctttcgagc acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt
    11581 taaccttttc ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag
    11641 caaatacgca taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc
    11701 tggctaaagt tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt
    11761 ctcttttctt acaccgcgcc gataaaaaat atgcacgttt attgcatatc tttcagtgtg
    11821 acaacttttg ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat
    11881 aaaaaatggc tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc
    11941 gcggctgaac aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg
    12001 gtggccaatt ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac
    12061 gaagtactgg aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct
    12121 gccagctttc tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa
    12181 ttgccggagt ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc
    12241 gacaataaat ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat
    12301 aaagttaaag ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa
    12361 cctgaaaatc tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa
    12421 gtttttgcta ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat
    12481 tacaccggac cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat
    12541 tcatctcaat acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca
    12601 ggtgatgtct ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg
    12661 ttctcgattc caaaagaagg ggcgatggcg ttctttgatg tattcgccat gcctgcggat
    12721 gccaaaaaca aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta
    12781 gcgcatattt ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg
    12841 agtgcggaag tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg
    12901 ttcactctga aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa
    12961 gtgaagagcg gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg
    13021 caattcgcac catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga
    13081 cgctatccct cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg
    13141 caacctcacc aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta
    13201 caaaggtgaa atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg
    13261 tatgctggca ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt
    13321 gtcacaggtt ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt
    13381 tccgcatatg accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa
    13441 agcggaaatt tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc
    13501 caaacgtaaa ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag
    13561 ccttgccaag cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa
    13621 gctgcgtgac cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg
    13681 tgtgatggtc acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat
    13741 gaatcgtggg aaatttgtcc agattggcga accggaagag atctacgagc atccgactac
    13801 ccgctatagc gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg
    13861 tcaggaagat ggcctggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc
    13921 ggatgcctcg gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat
    13981 gctttgcgaa gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat
    14041 tgcctatctc ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag
    14101 tgcccagcta caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt
    14161 gcgtttgtgc tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa
    14221 tacacttgaa cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca
    14281 gctgcaaatg aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt
    14341 gctgtttctg ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc
    14401 tattccacct tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa
    14461 tctcggtaat tttctgcaac tgaccgacga ttcgctctat ttcgatgctt atctccagtc
    14521 gctacaggtg gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg
    14581 ggcggtggcg cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc
    14641 gtcgtggacc tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg
    14701 tgtgctgaat aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca
    14761 taccaatctg gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc
    14821 gatttatacc gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg
    14881 tgcacgaccg ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat
    14941 tgccggatcg atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct
    15001 cggtggcccg gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg
    15061 cgactggccg gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat
    15121 aatgtggttc cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg
    15181 gtagttcgtt cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg
    15241 ccaatgctga tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtgggcc
    15301 ggctggtcaa cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt
    15361 ggttcaagcc tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt
    15421 gcggcggtgg tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg
    15481 atcaccgcgc cgctggtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc
    15541 gtcgcgcttg ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg
    15601 gcgcatgtca ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa
    15661 ctggatcgct cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt
    15721 ttcgtcatta cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt
    15781 actttgtcgc ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg
    15841 ttaccgatgc tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg
    15901 gcaacattga tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg
    15961 cgcgcagaaa aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa
    16021 aatttgccaa cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg
    16081 aaacgttggg attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc
    16141 tggtgcaggt ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt
    16201 tcaatacgct gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga
    16261 gtttaacgct ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt
    16321 tttcactggt gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg
    16381 ccgcaagtta cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc
    16441 ccggtgaatc aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc
    16501 tcatcctgat gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat
    16561 gtgtataatc gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac
    16621 gcgggtcgct gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct
    16681 aaaaccgccg atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg
    16741 gtgtcaggcc cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt
    16801 gaaaaaacac tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc
    16861 ccgctttatc cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga
    16921 gcggggttaa cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg
    16981 actgaaagcc agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag
    17041 ctggcgcaac tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt
    17101 attaccgtca atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac
    17161 ctgaccgaac aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg
    17221 caaagtttct tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac
    17281 tgggtacgac agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt
    17341 ggtttacact gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc
    17401 attgcctgtg caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg
    17461 ctggactcca ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac
    17521 ccgccgcgcc gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt
    17581 tttattatct actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca
    17641 ggttaccgca tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa
    17701 gtgttgacgc tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc
    17761 aggtgcgctt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg
    17821 ctaaatcaat acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct
    17881 tacttttgtc atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt
    17941 accgtcagct ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc
    18001 cggacgtaca gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt
    18061 cgcaacgccc agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa
    18121 taccccatca atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga
    18181 gacagttttc acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc
    18241 catcccaatg cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat
    18301 cacgacggct gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg
    18361 ggtgatatcc ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag
    18421 gctgtcgaac gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt
    18481 tgccagatcg atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg
    18541 ataggtggct gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga
    18601 agcaagtaaa gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat
    18661 gatttttggc cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat
    18721 aaacatgatt aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg
    18781 tcttcattat aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg
    18841 cacaggcacc gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc
    18901 ccccggcgaa tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc
    18961 gatcagacgc atcatcagcg tcagcaggcc gttgacgacc aggtaaataa tccctgccgc
    19021 accgaacacc attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc
    19081 catcagcgta atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga
    19141 ataagaagag agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt
    19201 gctcattccc agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc
    19261 gtaaaacagc tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa
    19321 ccacggttct gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc
    19381 cggcccgtaa taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat
    19441 ataaccccgc accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat
    19501 caatgccaga atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag
    19561 ccctttcatc agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc
    19621 gcgcgcaggt caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc
    19681 gccgccgcca caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt
    19741 tgtaacatta aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc
    19801 agccactggt taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg
    19861 aaaaagatag ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt
    19921 acagctttca acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga
    19981 gcgatgacac cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg
    20041 atctgcacga acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag
    20101 gagccaaaat agataaacag cactaccaga atttctggca ggccacgcag aatggttacc
    20161 agcgctgaac ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac
    20221 atcgccagcg ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg
    20281 gcgcttgcta aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag
    20341 atggtttcgt aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc
    20401 agttcagtgt tgccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg
    20461 gtcactttat cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact
    20521 gcggtgtcac caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg
    20581 tagctgtcat acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt
    20641 gtcgtcccgt tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg
    20701 ccttgctgac ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc
    20761 ttttcacgct ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc
    20821 aggcttggga tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct
    20881 ttacacagtg cctgtgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc
    20941 gattcaaacg gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg
    21001 gaaagactaa aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg
    21061 aatcttagtg agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg
    21121 cgtcgccttg ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac
    21181 gcgccacttc aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac
    21241 gaatgatgct gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga
    21301 acagcagtac ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac
    21361 caccagaaag atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca
    21421 gtttttctgc acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt
    21481 caatcaggtt ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc
    21541 caacgttacg acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat
    21601 ggttgcctgc aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca
    21661 gcagcgagct tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc
    21721 aatccagcgt gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta
    21781 attgaatact cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag
    21841 tacctttgac agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt
    21901 attcaataaa catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat
    21961 tcctcacgaa atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc
    22021 tcgatggact gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca
    22081 tcgtcgatga cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg
    22141 tcaccctgtt tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc
    22201 gatgcactgg caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg
    22261 cttaacaatt cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg
    22321 tcactcaaat aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg
    22381 tagaattgtt gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg
    22441 ttatctttat aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata
    22501 agtagagata gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac
    22561 actacgacca aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa
    22621 tctgaacacc tgattgggtt tgagtccatt gctggctacc cactcaaggt tgctcgtagc
    22681 tgacttactt aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt
    22741 tatatggctg aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc
    22801 ctcctgtaga ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa
    22861 atgaatgcgg ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg
    22921 attaagatga ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc
    22981 agctttgact ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat
    23041 gaatttgagt gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa
    23101 aaggcgttgt gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt
    23161 gggtgatttt taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac
    23221 aactaaatca aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatt
    23281 ccagtcgtaa gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa
    23341 taaatgctga cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag
    23401 cgaattacca atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc
    23461 tgggtaaatc agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg
    23521 gaatacgtaa tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact
    23581 atgagaataa attacaagaa caattagata agtattttac gattgaatcg cacccattat
    23641 tatacaggta taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc
    23701 gtattgactt taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt
    23761 atgattatag caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca
    23821 atatcgcatt gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata
    23881 atgattttac agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc
    23941 ttggatttgc tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac
    24001 gatttagtgc ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt
    24061 accacgatat ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact
    24121 tgatggtgaa tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg
    24181 aagatgcaag taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat
    24241 atagtcattt taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac
    24301 acactgttgt gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg
    24361 atgttaaaat aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgtttagat
    24421 ctgttaaaat taataacgta aatactcatt ctttaaaatt tattaactgc aattttttca
    24481 atgttgatat gataagagtt aatctcagca aatgtctttt tcatgaatgt agtatgcatg
    24541 gtgtcaagat aaaaccctgg ttgcctgtaa aatggacaaa agaattaata agtgattatt
    24601 tgtatggctg tttactatct ctgtactcta tctgtgcaag ggatatatat aatatgaacg
    24661 ccgggaataa cgtgaaggtt gctgcggatg ctttccttga aattattttc tctcttaaaa
    24721 ataaatattg tattaaacta ttaagcgctc aggatcgggc gtttatatac gagtttgcaa
    24781 gaatgatctt cgcttatatt aatgacaaaa gcattgagat cttattactt tcttgttttg
    24841 cggcggctga ccaaaaagca atccagcggt atagacctca gtcacaagat ggggaagact
    24901 ttaggagtca tttacaatat aagttgcctc tttccgctca ttaatgttgt tggtgcaaca
    24961 taataagaca ggtaagcggt accctataat gaaaccaata tcgggttatg caacggataa
    25021 tcgtaaagac ttaagtgggt gtttttgaca caatgagatg atttcctaaa ctgatcaaaa
    25081 ccttcttccc aacatggcat atcagtccga taatgcttta gagatcgaag gcttaacaaa
    25141 caaggagatc gatcatgcaa ttttctacaa ccccaactct ggaaggcctg accatcgttg
    25201 aatattgcgg tgtggtgacc ggcgaagcga ttttaggtgc caatattttc cgtgatttct
    25261 ttgccggtat ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa gaactgcgta
    25321 aagcacggga gatcgccttt gaggaattag gctcccaggc gcgggcgctg ggggccgatg
    25381 ccgtagttgg tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg
    25441 ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc
    25501 tgccgctctg ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata
    25561 tcagcttgat acccgacacc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat
    25621 ccactacacc gcagatgatt ttgatagctc gctggcgaca ctgactgata agcaggtcag
    25681 ctcgcattat ctgatccctg cggtgccacc gcgatacaac ggtaaaccgc gcatctggca
    25741 actggtgccg gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac
    25801 gcgccttaac gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcatc
    25861 cggagtgaaa tattttgccc cgtttgaaca ggcacagatt caggcgctta ttccgctggc
    25921 gaaagatatt attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat
    25981 cgcaccgcag cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca
    26041 ggggattggt gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc
    26101 gcacacaccg gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt
    26161 aaaacctgat atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc agatgcattt
    26221 ccgcccgacg ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcatt
    26281 gctggagaaa tacgggcagg attagcgcgg cagttttccg tggtcgcgta gccaggcggc
    26341 agttttctcg atgccttcat ccagggtgat gaccggctga taacctaact cttcctgcgc
    26401 acgcgtaata tccagcgtaa agtcaaaatt caacttggag acgccgtagt gggtcagcgg
    26461 cggctctttt gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat
    26521 cgggtaaggg acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac
    26581 gatgctgcgc agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag
    26641 cttatcgcag gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt
    26701 catatcgacc agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat
    26761 atgcgccaga cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag
    26821 aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc
    26881 tgctttgctg cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt
    26941 gcgatggtgg tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac
    27001 accccaggcg acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa
    27061 agcctgttgt gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc
    27121 cgcgagcatc actttagctt gtgatgaaac cagctcggtc agatccgtcg gaacaaactc
    27181 tgcgcccatt ttttccagca atttacccat tgcttcgttg cgaccgctcg cacgcacgct
    27241 gatgcctttc tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt
    27301 aaccagtacc ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg
    27361 atttccccca tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct
    27421 gtgctttgtt ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc
    27481 gcggggatca tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg
    27541 cggacatcga tagtacgata gtcaccttta tcttccaggg taaaacacag tcgtcccagc
    27601 cccggcgctt tcatgccaaa taacaacgta agttgctttt tcggttcaac gacaatcact
    27661 ttccagctat ccaccgcatc gccggtctgt aaatattcgc gctccgggcg gcctttcgcc
    27721 agattatgac cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag
    27781 aaataacgct ctttaccgcc gatttggttc accaccagcc ataaagccgc aaggctggcg
    27841 gacgttttaa cggtaaaccc cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg
    27901 gcaaaggcct gagcgtcgta gccccagtcg ctggagttga ccagtttttc ttcctctttc
    27961 aacgtgctac gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc
    28021 gcggtatcat ctgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg
    28081 ggcggtacgg aagtaatcac attgagaaac cacaccgaaa tccagcgggt ggggaggggg
    28141 atggggatca accagcggcg cttaccgctc accgccataa aatgttcaaa ctgttgctga
    28201 taactgagca cctctggtcc ggcggcttcg aagatgcggt gttcgttggc tggatggtct
    28261 aacagcgcca ccagatagtg cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt
    28321 acccagcgtg gcggcgttaa cactggcagg ttgtagacca tatcgcgcat gacttcgaac
    28381 gccgctgaac ctgcgccaac gataattccg gcccgaagtt cggtcactgg tacattcgct
    28441 tcacgaagaa tgtccgccgt agcctgacga gctcgcagat gatccgactg ctcatgtggc
    28501 ggggcctgca acgaactgag aaagattaat tgcttaactg gtacttcacg tagcgcatcg
    28561 cggacgttga gagccagctg gcgctcctga gcgataaaat cgccgccttc gcccatgctg
    28621 tgcaccagaa aatagacggt atcgatatcc tgcaacaggg ccggaaggtt atccggccag
    28681 ctgagatcga ctttatggca actgacgttt gccagttgca gctttgcaag cctgtcgaca
    28741 tgacgtgccg ccgctaggat ctgatgccct tgctggctga gtgtgcgcac cagatgctga
    28801 ccaatgtagc cactggcacc gagaactaaa atgcgttgcg gcacgtatct ctccttaacg
    28861 cgccaggaat gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag
    28921 atgcgtcacc aggcggacaa tcggcgaggc gttaatcagc acgtttctcg ctttcatgta
    28981 ttcgcctaac gcggcagcat tttcttcccc gacgcgaaca aacagcatat tagtgtcctg
    29041 acgcatcaca tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg
    29101 atcttcctgc aaccgtgcga cgttattttt cagggcatac atcccggcgg cagccagaat
    29161 cccggactga cgcatcccgc caccggccat tttccgccag cggatggcac gtttaatgta
    29221 atcacgatta ccgacgagta atgaaccgac tggcgtccca agacctttcg acaggcaaat
    29281 ggtgaacgaa tcacaatatt gcgtgatctc tttcagttcg cagccgtaag ccaccacggc
    29341 attaaagatg cgcgcaccgt caacatgcag cgccagattg cgctcgcggg taaattccca
    29401 tgcttctttc aggtattcac gcggcagcac tttgccgttg tgggtgtttt ccagactgag
    29461 taatttggtg cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag
    29521 cggtagcgtg ccgtcggcag ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc
    29581 tgcgccaccg gcttcaaaca gatagttatg cgcggcctga ccgacaatat actcttcgcc
    29641 acgttcgcag tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat
    29701 ggcggcttct ttaccggaaa gctctgctgc atagtcctgc agagcattaa cggtagggtc
    29761 gtctccgtaa acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct
    29821 cggtcgggta acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa
    29881 atgccaccct ttttacctta gccagttcgt tttcgccagt tcgatcactt catcgccgcg
    29941 tccgctgata attgcgcgca acatatacag gctaaaacct ttggcctgtt cgagtttgat
    30001 ctgcggtgga atggctaatt cttctttggc gaccaccaca tccaccaaca ccggaccgtc
    30061 gatagagaag gcgcgttgca gggcttcatc aacttcagag gctttttcta cacggatacc
    30121 cgtaatgccg cacgcttcgg caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc
    30181 tgtcaggtaa cctccggcct tcatctccat cgccacaaag cccagcacgc tgttattaaa
    30241 gacgataatt ttcaccggca gtttcatctg cactactgag aggaaatcgc ccatcaacat
    30301 gctaaaaccg ccatcgccgc acatggcgac cacctgacgt tccggctcgg ttgcctgcgc
    30361 acccaacgcc tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg
    30421 acgcttgccg ttcattttca gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca
    30481 ggtaaagatg gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg
    30541 aatggctttc tcgctcggtt tagctaaatc gtccagccct ttgcgggcat cgcggtaatc
    30601 ttccagcgct ttatccagaa acttgcgatc ggttttttct tccaccagtg gcagcaatgc
    30661 gcgcagggtt gatttgatat cgccgaccag cgccatatct accttgctat gcgcgccgat
    30721 gctggctgga ttaatatcaa tctgaataat tttggcatcg gtcgggtaga acgcacggta
    30781 ggggaactga gtaccgagta gcactaacgt gtcggcgttc atcatggtat ggaaacctga
    30841 cgagaatccg attaacccgg tcattccaac atcatacgga ttatcgtatt cgacatgctc
    30901 tttaccgcgt agggcatgaa caataggtgc tttaattttc ccggcaaact caactaactc
    30961 tttatgcgcc cccgcgcagc cgctgccaca catcagggcg atattgctgg aataacgcag
    31021 cagttgcgcc agtttgcgta actcttcttc ttccggcatc acgactggct gtggcgcatg
    31081 ataccagtgc gtggttgccc cttctggcgc aggttttaac gccacgtcgc ccggtaatac
    31141 aacaacggaa acgccacggt taagcaccgc tttgcgcata gcaattgcca gcacttgtgg
    31201 gatctgctcc gggctggaaa ccagctcgca atagtgacta cattcgcgga atagctcttg
    31261 tgggtgggtt tcctggaaat agccgctgcc aatttcgctg gagggaatat gagcggcaat
    31321 cgccagtacc ggaacgttat tgcggtggca atcgaatagg ccgttgatca agtgcaggtt
    31381 gcctgggccg cacgatccgg cacagaccgc cagttctcct gaaagttgtg cttcagcgcc
    31441 agcggcaaac gccgccactt cttcatggcg ggtggacatc cactcgatgg tgcccatgcg
    31501 attaagactg tcgctaagac cgttcagaga gtcgcccgtg actccccaga tgcgtttcac
    31561 gcctgccgat tcgagtgttt tggcgatata agctgcaacc gtttgtttca tggttctcca
    31621 tctcctgaat gtgataacgg taacaagttt agttcatctg acggaggggg aagggatggg
    31681 agagaaagga ggcactaacg gttaaatagc ccgatgaaag gaatatcatc gggcataagg
    31741 cgattatgcg agaaccaaat ccccctgcgg atggcaggag caggccagta cgtaaccttc
    31801 agcgatttcg gcgtcggtca gcgtcattgt actgctcacc gtatattcac cggaaaccac
    31861 ttttgtctta cagcagccgc aaacacccgc acggcaggca gcgacaaccg gaacgttatt
    31921 gctttccagc gcctccagta gcgtggtgcc aaccggggcg taaaattctc gtgccggttg
    31981 cagtttggtg aatttcagac cgctggtcgc cgcttctgct actggggtga agaatttctc
    32041 tttaaagaaa cgcgtcacgc cgagcgcttt cacttcctgc tctacccaat ccatatacgg
    32101 agccgggccg caggtcatca cggtacgtga agctaagtca ggtacatctg ccagcagttc
    32161 gcgagtgaga cgaccagcga taaagccttc ggtaacgtta ttttctgcca ccagcgttac
    32221 cggatagtta cgccactcat cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat
    32281 cacccgcaca tcggcctgtg gacggttctt cgcaagccag cgacgcatcg acataatcgg
    32341 cgtgacgccg cagcctgccg ccagcaacag gaatttatct tctgctttat cgtcgcaggt
    32401 aaattccccc atcgcgtccg aaagccagag ataatcaccg cgttttacat cgcgcgtcag
    32461 ccactgggag ccgacaccgt catcaatccg ccgcacggtc agggtgatat attcactcac
    32521 gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac
    32581 cagtgcatat tgcccggcgc gatatgggta gtgatcgtgg caaatcaggg aaatcgtcca
    32641 cacatccggc gtttcttgcg taatgtgatg aacctgtatc cgccacgggc attgattcgt
    32701 tggcatcgtc atcgacaaac tccttacgcg ttcaacagtt gcttcatgtc ttcttcaaca
    32761 gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg
    32821 aaaccaggtg cagtcgggcc ggtgacgata tttttcacgc ccagagaaag cagcgtcagc
    32881 agaatgacaa tcgctttctg ttcaaaccag gagagcacca gcgacagcgg cagatcgttc
    32941 acaccacagc ccagtttctc tgccagagtg acagccagaa taattgctga gtaagcatcg
    33001 ttacattgac ctgcatctac cagacgcggc agaccttcga tatcgccaaa ctcaagtttg
    33061 ttaaagcgat atttaccaca ggcgagggtc aggatcaggc agtcatccgg cacgctggtg
    33121 gcgaaatcgg tgaagtagtg gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag
    33181 atatgacgca gtttttcacg gctaaccaga tcaatcagcg tatcggctgc accaagcagc
    33241 gtctggcgac caaagcccac ggtgatcagg tgaggaattt cgctgtacgg gaagcccgcc
    33301 atttgttgcg cctgggcgat gaccgcagag aaatcttcgc catccagatg acgcacgcca
    33361 ggccagccaa cgatgctgcg ggtccagata cgatcgtcat aagcgcctac ggttgggtcg
    33421 atgatgcagt tcgaggtcat cacgatgggg ccagggaaac gagcgaactc cacttgctga
    33481 ttctgccagc cgctgccgta gttaccgacc agatgcttga atttacgcag ctccgggtag
    33541 ccatgcgcag gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt
    33601 tccagcaggt tgtagagatc tttgagatcg tgaccggaaa tcagaatgca tttacccgcc
    33661 gtcgctttga cgttgacctg ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca
    33721 tccagaatgc tcatcacttt gaagttcatc tggccgattt ccattgaaca ctcaagaagc
    33781 gcgttcatat cggcaggcca ggtccccagc cacgccatga ttttatggta ctgggcataa
    33841 atatcgttgt cgtattgacc gagaacatgc gcgtgttcca tataggccgc cgcacctttc
    33901 aggccataca ggcacagcag acgcaggccg agaatgtttt cgccaatcgc cgctttatct
    33961 ttgttagggg taaattctgc tgcctgacgt tgcagctcgc cgagatcatc gctcaccagt
    34021 tgcaggtcag ccatcgggtt atcgacgcgc gcgttggcat ctacagccag gcattgtgct
    34081 ttcagcgcct cgcgcaaggc aatcgcttca cgagcgtagc cgacaatacg cggagaatcg
    34141 aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg
    34201 ttgatgatgc cgtattcacg cgcttttacc gcccaggcag aaagcccttg cagcgccgcg
    34261 atgagtaaat cctgaaggtc agaagtttcc gccgttttac cacacatccc ctgcgcgtat
    34321 gagcagccgt tttctgccgg agtacggata gtttgttcac attgcacaca aaacatgatc
    34381 acacctttta aagttatatt taatatacat gtttaagggt aagacgctta acgcggggat
    34441 aaaagggatt tttcatgcaa ctttaaggga gattgattta gcgcaatttt ggcggcaggg
    34501 aactaccgcc agagaggtat tacgcagaga aaaaggcgat gaggatcggc actaacaggc
    34561 taagaataaa accgtgaaca attgccgccg ggaccatatc cagcccgcca gtacgttgaa
    34621 gaacgggcag ggtgaaatcc attgatgtgg caccgcataa gcccagtgca gtagagcggc
    34681 tgcggcgaat cagcccaggg atcaacataa tagcaatcag ttcacgggcc agatcattaa
    34741 aaaacgccgc gctcccgatt accggaccaa aagattcggt caataaaata ccggaaagag
    34801 aataccagcc gaaaccggag gccattgcca gcgcggtatt gatggggaga tcaagaataa
    34861 aggcgttaat taaaccacca attaatgaac tgacaaccac caccacggcg acaatcattc
    34921 cccggcgatt aaggacaatc tgctttaagg tcatgccatt attgcgcaac tgaataccaa
    34981 cgaggaaaag tagcaaaatt aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag
    35041 ccagtccact cagaccaatg gcaaaaccaa tcactactac gccgcacagt tttagcgact
    35101 ccagcgccat cgcaatacgc gacgggagtt tttcttgctg atggtggttg cgccacggca
    35161 ggcctcgctc cagccacatc agggcggcaa tattacacag taaaataacg gtaatactga
    35221 cggcagaata atgcagaatc gccaacaggt tactggcgag gttatcgaga aacgccagac
    35281 tgatacccat aaaaaagaga ataaggtaaa ccatccagct taatagctga ttaatagctt
    35341 ttaacgcagc ttgttggcga agcggaatga ggtaacccac aatcagggga accagaatga
    35401 ttaacagccc agaaaacatg aaaatccagt ccttgcaaag aagaagtcga aatgcgcgat
    35461 gacacactac tgaaagcgga agggcgagta aagttgcaat taaaaggaaa tgttatgcat
    35521 cagaggggga tttttaatcc tatctgagtg tttagcattg ttgcgctcct ttcatgtgca
    35581 atgccatata aagccactgg cggagggagc gcgcaggggg cagccagtcg ccgccgcccc
    35641 ctgcaccccc gggctctggc gaacaaaatc gccgctgcgc ggtaccttca gcttatcccc
    35701 ttcggcaacc gggtcgggcg cgaggtaacg tcctgtaaga cgcgccctca gcccacatcc
    35761 atgtgggctg ccccggcctt cggggaacgc ttcagcgatt ttgacgccac caatacccgt
    35821 gcggtttgta atgtcgaaga taaaacaact agggcagata aaatgccaat gctaattaat
    35881 cacgcttttc cagcagagtc cggtaaatca gaccaccgat aatgccgccg acaattggca
    35941 ccacccagaa gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg
    36001 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat actgattaag tgaatcaggg
    36061 ttaaggccag accaatagcg atcggcgcaa aacctgccgg agcgaatttg tcggttgcac
    36121 cgtggatcac taacaggaag cccgcactca ataccagttc aactaccagc gcggaaagca
    36181 tggaataacc gcctggtgaa tgctcgccat aaccgttaga agcaaaaccg ctggctgccg
    36241 cgtcaaaacc cgttttacca ctggcaatta aatacagcag cgccgctgca acaataccgc
    36301 cgacaacctg ggcaattacg tagccaacga cttcttttgc cggaaaacgt ccgccagccc
    36361 ataaaccaat agtgaccgcc gggttaaaat gaccaccaga aatatgacca acagcgaagg
    36421 ccatcgtcag aacggtcaaa ccaaatgcca gcgccacgcc ggcaaaacca atgcctaatt
    36481 ccgggaagcc tgcggccagt acagcactac cacagccacc aaaaacaagc cagaaagtac
    36541 caaaacattc agctgctaat tttctgaaca tatccacctc aattaaaaat tgaccctgtg
    36601 aaaa
//
LOCUS       DAJTRB010000032        36330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTRB010000032 DAJTRB010000000
VERSION     DAJTRB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.032"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO330_001725"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO330_001725"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5882625.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO330_001726"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO330_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5882626.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO330_001727"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO330_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5882627.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO330_001728"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO330_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5882628.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO330_001729"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO330_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5882629.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO330_001730"
     CDS             5377..5835
                     /locus_tag="OO330_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5882630.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO330_001731"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO330_001731"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5882631.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO330_001732"
     tRNA            6887..6971
                     /locus_tag="OO330_001732"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO330_001733"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO330_001733"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO330_001734"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO330_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5882632.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO330_001735"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO330_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5882633.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO330_001736"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO330_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5882634.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO330_001737"
     CDS             complement(10252..13995)
                     /locus_tag="OO330_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5882635.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO330_001738"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO330_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5882636.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO330_001739"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO330_001739"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5882637.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO330_001740"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO330_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5882638.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO330_001741"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO330_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5882639.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO330_001742"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO330_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5882640.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO330_001743"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO330_001743"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5882641.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO330_001744"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO330_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5882642.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO330_001745"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO330_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5882643.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO330_001746"
     CDS             23392..23499
                     /locus_tag="OO330_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5882644.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO330_001747"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO330_001747"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5882645.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO330_001748"
                     /pseudo
     CDS             complement(24538..25347)
                     /locus_tag="OO330_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            complement(25351..25743)
                     /locus_tag="OO330_001749"
     CDS             complement(25351..25743)
                     /locus_tag="OO330_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5882646.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO330_001750"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO330_001750"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5882647.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO330_001751"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO330_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5882648.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO330_001752"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO330_001752"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5882649.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO330_001753"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO330_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5882650.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO330_001754"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO330_001754"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5882651.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO330_001755"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO330_001755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5882652.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO330_001756"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO330_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5882653.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO330_001757"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO330_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5882654.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO330_001758"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO330_001758"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5882655.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO330_001759"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO330_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5882656.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         8955 a         9464 c         8903 g         9008 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttatattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcagcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggca cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 tcccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccaa gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagaggcagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcattcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctacg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca
//
LOCUS       DAJTRB010000033        35233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTRB010000033 DAJTRB010000000
VERSION     DAJTRB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.033"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..76
                     /locus_tag="OO330_001760"
     CDS             <1..76
                     /locus_tag="OO330_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5882657.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            83..2680
                     /locus_tag="OO330_001761"
                     /pseudo
     CDS             83..2680
                     /locus_tag="OO330_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
     gene            2677..3765
                     /locus_tag="OO330_001762"
     CDS             2677..3765
                     /locus_tag="OO330_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5882658.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3853..5151)
                     /gene="exuT"
                     /locus_tag="OO330_001763"
     CDS             complement(3853..5151)
                     /gene="exuT"
                     /locus_tag="OO330_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5882659.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5634..7046
                     /gene="uxaC"
                     /locus_tag="OO330_001764"
     CDS             5634..7046
                     /gene="uxaC"
                     /locus_tag="OO330_001764"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5882660.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7061..8548
                     /gene="uxaA"
                     /locus_tag="OO330_001765"
     CDS             7061..8548
                     /gene="uxaA"
                     /locus_tag="OO330_001765"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5882661.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8631..9182
                     /locus_tag="OO330_001766"
     CDS             8631..9182
                     /locus_tag="OO330_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5882662.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(9187..10431)
                     /gene="sstT"
                     /locus_tag="OO330_001767"
     CDS             complement(9187..10431)
                     /gene="sstT"
                     /locus_tag="OO330_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5882663.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(11027..11992)
                     /gene="alx"
                     /locus_tag="OO330_001768"
     CDS             complement(11027..11992)
                     /gene="alx"
                     /locus_tag="OO330_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5882664.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(12263..13261)
                     /locus_tag="OO330_001769"
     CDS             complement(12263..13261)
                     /locus_tag="OO330_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5882665.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSHSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(13340..14032)
                     /locus_tag="OO330_001770"
     CDS             complement(13340..14032)
                     /locus_tag="OO330_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5882666.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(14109..14612)
                     /locus_tag="OO330_001771"
     CDS             complement(14109..14612)
                     /locus_tag="OO330_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5882667.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14697..15833
                     /gene="rlmG"
                     /locus_tag="OO330_001772"
     CDS             14697..15833
                     /gene="rlmG"
                     /locus_tag="OO330_001772"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5882668.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            16118..16432
                     /gene="higB"
                     /locus_tag="OO330_001773"
     CDS             16118..16432
                     /gene="higB"
                     /locus_tag="OO330_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5882669.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16429..16845
                     /gene="higA"
                     /locus_tag="OO330_001774"
     CDS             16429..16845
                     /gene="higA"
                     /locus_tag="OO330_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5882670.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16890..18908)
                     /gene="fadH"
                     /locus_tag="OO330_001775"
     CDS             complement(16890..18908)
                     /gene="fadH"
                     /locus_tag="OO330_001775"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5882671.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGSAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19134..21485)
                     /gene="ygjK"
                     /locus_tag="OO330_001776"
     CDS             complement(19134..21485)
                     /gene="ygjK"
                     /locus_tag="OO330_001776"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5882672.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21502..21921)
                     /locus_tag="OO330_001777"
     CDS             complement(21502..21921)
                     /locus_tag="OO330_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882673.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21899..22252)
                     /locus_tag="OO330_001778"
     CDS             complement(21899..22252)
                     /locus_tag="OO330_001778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882674.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(22253..22573)
                     /locus_tag="OO330_001779"
     CDS             complement(22253..22573)
                     /locus_tag="OO330_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882675.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22707..24140)
                     /locus_tag="OO330_001780"
     CDS             complement(22707..24140)
                     /locus_tag="OO330_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5882676.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGHWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(24203..24652)
                     /locus_tag="OO330_001781"
     CDS             complement(24203..24652)
                     /locus_tag="OO330_001781"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5882677.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24649..27735)
                     /gene="ebgA"
                     /locus_tag="OO330_001782"
     CDS             complement(24649..27735)
                     /gene="ebgA"
                     /locus_tag="OO330_001782"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5882678.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRMMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27918..28901)
                     /gene="ebgR"
                     /locus_tag="OO330_001783"
     CDS             complement(27918..28901)
                     /gene="ebgR"
                     /locus_tag="OO330_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5882679.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29119..29450
                     /locus_tag="OO330_001784"
                     /pseudo
     CDS             29119..29450
                     /locus_tag="OO330_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29492..30871)
                     /gene="ygjG"
                     /locus_tag="OO330_001785"
     CDS             complement(29492..30871)
                     /gene="ygjG"
                     /locus_tag="OO330_001785"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5882680.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            31289..32809
                     /gene="aer"
                     /locus_tag="OO330_001786"
                     /pseudo
     CDS             31289..32809
                     /gene="aer"
                     /locus_tag="OO330_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32963..33586)
                     /gene="nfeR"
                     /locus_tag="OO330_001787"
                     /gene_synonym="yqjI"
     CDS             complement(32963..33586)
                     /gene="nfeR"
                     /locus_tag="OO330_001787"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5882681.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33863..34608
                     /gene="nfeF"
                     /locus_tag="OO330_001788"
                     /pseudo
     CDS             33863..34608
                     /gene="nfeF"
                     /locus_tag="OO330_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34662..34737)
                     /locus_tag="OO330_001789"
     tRNA            complement(34662..34737)
                     /locus_tag="OO330_001789"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34702..34704),aa:Ile,seq:cat)
     gene            34862..35212
                     /gene="mug"
                     /locus_tag="OO330_001790"
                     /pseudo
     CDS             34862..35212
                     /gene="mug"
                     /locus_tag="OO330_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8633 a         9017 c         9238 g         8345 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac ccagggacag caggagtata tcgaggttta
      121 tttttcaggg aaaagtctcg gtaaatatta tgcaatggtt aatcttgata ccgtaacatt
      181 tcttgatcca gcaagtctat ataacaagct ggaactggat gtagacgatc agaaaatcgc
      241 gcatatagtg aaagaaaaat tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg
      301 ttatgtacgt actgactcag ggtgtggttt tctgaatacc gatacgctgg aaataatcta
      361 taatgatgaa gaaagttcgg caacgttgtt tattaatccg caatggaatt cagctttcga
      421 tgcgaagtca ttatatttaa atccagacaa aaatactgtt aacgctttta tacatcagca
      481 agacatcaat gttctggcac aggatgatta ccaatcgttg tctattcagg gaaacggtgc
      541 gctgggaata acagaaaata gctatattgg tgcacactgg aatttcaacg gttatgatgc
      601 agatgatgtc agtgacagta atactgacgt cagcgatctc tattatcgtt atgatttttt
      661 acgtcgttat tatgtgcagg cgggtcgcat ggacaaccgc acactattta atgcacaagg
      721 cgggaacttt acctttaact ttctgccact cggtgcaatc gatgggatgc gtatcgggtc
      781 gacactgagc tatttaaacc aggcgcaaag ccagcaagga accccagtaa tggttctgct
      841 ttcgcgcaat tctcgtgttg acgcttatcg taatgagcaa cttttgggat cgttttatct
      901 caatagtggt tcgcaattta ttgataccag ttcctttccg ccaggcagct acagcgtggc
      961 attaaaagtc tacgaaaata accaactcac ccgcaccgag cttgtgccgt ttaccaaaac
     1021 cggcggtctg actgacggaa atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca
     1081 ggtttctgat gatgaaagtt cagcttatca gctaggagta cgcctgccat tacatccgca
     1141 atatgagctc tacgcagggc tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa
     1201 taactggacg gcagatttag gcagggtggg gaatcttgca atcagcgcca gcgtgttccg
     1261 taacgatgac ggcggcaaag gtgatatgca acaggccaac tggagtaatc cgggatggcc
     1321 gacgttgggc ttttatcgga ccaactctga cggcgatgct tgtacaaccg acagcagaga
     1381 gagctataac gccttaagct gttatgaaag tatttccgcg acagtttcac tgaattttgt
     1441 cggctggaat atgatgctgg gttatacctg cacacaaaat aacactgatg atagtttgcg
     1501 ttgggataaa cagcagagct ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat
     1561 ctctgaaact gtacaactta gcgcttcccg cgctattgtg atgcgtgact ggattttgag
     1621 tacgtcagtc ggtgttttcc atcgtaatga caacggtggc gataacgacg acaacggctt
     1681 gtacttatcg ttttcgttat ctgacacgcc aacgatggat agcaataaca acagccattc
     1741 aaccaatgtg cctacggatt atcgttatag cgaacaggat ggcgatcaaa cgtcatggca
     1801 gttatcgcat accttttata acgattcatt cagccataaa gaacttggcg taaccgtcgg
     1861 aggcctgaac accgatacca taaacagcgc ggttaacggg cgttgggatg ggcaatacgg
     1921 aaatgtctac gctaccgtat ctgatagtta tgaccgtaag aatcatgatc atctttcggc
     1981 ctttacgggc acttatagct ctacgctggc tgtcagtcgc tatggcgtta atttgggagc
     2041 cagtggtaca gacgatttgc tgggtgcggt actggtggat gtgaaaggct tctctgaaca
     2101 ggatgaagag agtcaggatc tgcaactcga agcgcgggtg gcaggcagcc gaactttgca
     2161 gcttggtcaa agtgacagtg tgttgttccc ttatcctgga tttcagtctg gttttgttga
     2221 ggttaacgac agtagccagg gcaatcagca ggggacaaca aacatcatta acggtgcagg
     2281 gaatcgtgaa ttaatgttgt tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt
     2341 taactacaac tatatcggtc gtttattact gccggcagcg gtgaaaaaat tcccgattgt
     2401 ggggctgaac agcgccatgt tactggtagc tgaagatggc ggatttacac ttgaaattaa
     2461 cggcagcgaa aaagagctct atctgctttc cgggcagcaa ttccttaagt gtccactgag
     2521 tgttgtaaag aaacgcgcca gcattcgtta cagcggagat gttacttgta gtgtggtgac
     2581 ttattcacaa ttaccggagt cgattcaggt tcaggcacag ttgaaacagc ctaaattacg
     2641 tggaaacgtt cagacggcgc aaagggaggt tgcaccatga gaaatcgatt gattgcggcg
     2701 atattgggct tgtttggcac gctcactggc gttcaggcag ctcctgacgt gaccagtgaa
     2761 attacgtatg atttggcatc tggtagagcg gattattact tctggaagga tgaagcgtcg
     2821 gcaggaaata atggatatat gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt
     2881 acagctaatg gaaatatatc gacagtacaa atctatttaa ctgaacaacg cagtgggatg
     2941 cgttggccgg taaaactcaa aggatttaaa acagccattg taagtagcga tgaagcgccg
     3001 ctaggatgca aggggggcaa agggcttcag acgaatctta aggattctaa tagatcttca
     3061 tgtacagaag atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt
     3121 gagcagacag agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta
     3181 cattcgaaca gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc
     3241 aaccatattg acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac
     3301 ttgcatccaa caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac
     3361 atgtgcctgt acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat
     3421 gaagggcgac cagctgcagg gcgcagagac ggttacttct ctatctatcg tcagggaggg
     3481 accaccaccg acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc
     3541 ggtgggcaaa ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa
     3601 cgtgtgcgtc cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc
     3661 ttgactctgg cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc
     3721 aaattgtcag taatctttac gccttccttg ccaaccatca attaaccctg caaaccggac
     3781 aaacgctttt ttctatataa acgacgaagc cgcctgataa ggcggcttcg tcgttataac
     3841 ttacgttaaa cgttaatgtt gcggtgcggg atcgttatgt gtttcctgcg ccacctcaat
     3901 cgccggtttg ttctgcaata cggtccagat aaccagcgca cccagcaggt caaatactgc
     3961 cagaactgcg aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc
     4021 aaacagcgta cttgccagcc atgcggacat cccggttaaa ccgttcgccg tcgccacttc
     4081 gttacgaccg aacacgtcag aagagagcgt aatcagcgcg ccagacagtg cctggtgggc
     4141 aaaaccaccg atacacagca gcataattgc gacatacggg ttggtgaaca gaccgatcat
     4201 acccgggcca atcatcagca cagcacccag cgttacgacc atcttacggg aaacgatcag
     4261 gttcacacca aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag
     4321 gtcagcaaac agcatcggca tccaggcgaa catcgcgatc tctttcaggt taaagccgta
     4381 aactttaaac atgaacagcg ggatccacgc gttaaaagta ccccaggccg gttctgcaag
     4441 gaaacgcggc agtgcgatac cccagaactg acggttacgc aggatctgac caacggacat
     4501 tttcttcgcc gtgctcacct ggtgctgggc ttcctgacca ttaataatat agtcgcgttc
     4561 ttcatcggtc agatgcttct ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc
     4621 ccagataaag ctcaatgcac cggagatgat aaatgccatc tgccagctgt gcatcacgat
     4681 tgcccatacc accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta
     4741 gcctactgcg atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc
     4801 cgggatcatc gctgcttccg cggcaccgac cgcaccacga gcaacagcta ggccacccca
     4861 gctacctgcc agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata
     4921 accgattttc gtacccagca catccagtac ataacctgct accggttgca tgaccgtata
     4981 agcagcagaa taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc
     5041 catcagagtt ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc
     5101 gagcgtcacc agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct
     5161 catcgttttg ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt
     5221 agttacgacc gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc
     5281 gtgctaattc ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca
     5341 atttgcattc cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacctt
     5401 aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg
     5461 aggccagtac tggcgcggcc tgcagcgaga tttaccactt tgagagtaat ttttttaact
     5521 acgtttattg atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt
     5581 tgtcagcatc gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc
     5641 cgtttatgac tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact
     5701 acgcaaaaga ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg
     5761 aagactatcg ttttaaaaac ctgtatgaca tctggttgaa gggcgatcac tacaaatggc
     5821 gcgctatgcg taccaacggt gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg
     5881 aaaaatttga cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact
     5941 ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg caaattgctt tctccgtcaa
     6001 ctgccgatga aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc
     6061 gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt
     6121 ctctggagca tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga
     6181 gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac ctttaacgac tacatggcga
     6241 agctgggcga agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga
     6301 ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg
     6361 tggtgatgtt tgctgaagcg aacgaagcgg aactggacag cattctggcg cgccgtctgg
     6421 ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc
     6481 ttggtgccga atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta
     6541 ataacaacct gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg
     6601 accgtccgat ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc
     6661 tgccgaaaac cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga
     6721 tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt
     6781 ttaacgatca gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc
     6841 tgagccgctt tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg
     6901 aatacttccg ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac
     6961 cggcggacat caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg
     7021 actacttcgc cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca
     7081 tgcgctggat aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt
     7141 cgataaccag actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac
     7201 ggatatcgca aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc
     7261 ggatattgcg gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct
     7321 ggatcagtat cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcctga
     7381 agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct
     7441 gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga
     7501 gaccaacaac gccgaaggta ccgacggcgt gttcctcttc agccacacct atggctgctc
     7561 acaactgggc gacgatcaca tcaacacccg caccatgctg caaaacatgg tgcgccaccc
     7621 gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt
     7681 ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca
     7741 ggatgatgag atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa
     7801 cgataaacgc gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc
     7861 tgacggtctt tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat
     7921 tgctaacggc ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt
     7981 gctgatggac cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga
     8041 cttcaaacag tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa
     8101 caaagcgggc ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg
     8161 ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa
     8221 cttgttaagt gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg
     8281 ccatatggtg ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt
     8341 gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc
     8401 gggtcagctg atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat
     8461 cgttgagttt gccaacggta agcaaacctg caacgagcgt aacgacttcc gcgaactggc
     8521 gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt
     8581 ttcataatgt gaagcgccgt ttgcttttcc atacctgcta aggatttatc atgaccgcgt
     8641 attggctggc ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca
     8701 atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac
     8761 atttttttct tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta
     8821 cattgattac cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg
     8881 gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga
     8941 ttgtcagtac ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt
     9001 tttcaacgtg ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacgatga
     9061 ttgagggtag ttttctgctt atgaatggcc tgaatatcat tcgtttctgg cggatgcaga
     9121 aaaggggaat tgatccgttt aaagtagaga aaacccctcc cgccatagac gaaaggggtt
     9181 aaacaattaa ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc
     9241 cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac
     9301 gccgataata aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat
     9361 attacaggcc agtgggatca gcagcaaaga accccccgcc acgccggatg cgccacaggc
     9421 acacagagaa gccactacgc tcaacagcag cgctgtgggc agatcgaccg gaatacccag
     9481 cgtattaacc gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat
     9541 ggtggctccc agcggaatag aaactgaata ggtatcgcga tccagattca gcttttcaca
     9601 cagcgccata ttcaccggaa tgttcgctgc agagctgcgg gtgaagaagg catacacacc
     9661 gctttcgcgc aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac
     9721 cagcaatggg ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag
     9781 ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagccc
     9841 aaaaataccg aacggtgcga agcgaatgac cagtttcacc ataaaggtaa cggcattcga
     9901 catatcattg accaggtttt tggtggtctc gttaccgtga cgcagtgcga agccgaggcc
     9961 gatcgcccac accagaatcc cgatgtagtt acctttcagc agggcgtcga tggggttgga
    10021 aaccatgctc attaccagtc cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc
    10081 acccgcgcta ctggacaagt gcagggtaga agggaaggca aagctgaaga ctactgcggc
    10141 cagagctgca gagaaggtgc ccagcagata gaggaacaaa ataggacgga tattggtttt
    10201 ctgcccgtgc tggtggttag caatagatgc cattaccagc atcaacacca ggatgggggc
    10261 aacagctttc agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg
    10321 ttttgagatc catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag
    10381 gctgccatga gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc
    10441 ctttcattgt gttgtcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa
    10501 caaagtgttt gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg
    10561 gattttaaag agaggtcatg ttttgcaact taatggttgc atttatttaa cataaacaga
    10621 gggaaggact ggctactaag gctgattaga taacatatgg gcaaaactat ttggaatatg
    10681 agaacggttg cgctccttta acgtgttatg ccatataaag tcactggcgc agggagcgcg
    10741 cagggggcgg ccaatcgccg ccgccccctg cacccccagg ctctggcgaa caaaatcgcc
    10801 gctgcgcggt tccctcagcc tatcccttgc ggctaccggg tcgggcgcaa ggtaacgtcc
    10861 ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt cggcgatttt gacgccacca
    10921 acacccgtgc ggtctttttg taaagagcta taagccgatt acataattaa tagctcacct
    10981 ttgtctggaa tgagttatct ctaggaatgg cttaatatac agcgaatcat tctccccgct
    11041 gcttatcatg ccgataattc acccaggcgt tgataataaa cgtcatcacc agaatgccaa
    11101 acaccacgcc cagcgagacg gcgattggaa tatggtagaa gtcgacaatc agcatcttga
    11161 taccgataaa caccagaatc accgccaggc catatttgag catcgagaaa cgctctgcta
    11221 cgcccgccag caggaaatac atcgcacgca ggccgaggat tgcaaacagg tttgaggtca
    11281 gcacaatgaa cgggtcagtg gtcacggcga agatagccgg aatgctatcc acggcaaaaa
    11341 tcacgtcgct caattccacc agaatcagta ccagcatcag cggtgtggcg tacagcaatc
    11401 cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc
    11461 gtagccagcg caccagcggc ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca
    11521 tcttcacgcc ggtaaacagc aggaaggcac cgaagatata caggatccag tcgaactgcg
    11581 aaatcagcca gctgcctgtg aagatcatga tggtacgcag aacaatcgcc ccgagcacgc
    11641 catacaccag cacgcgacgt tgtaatgccg ccggaacgga gaaatagctg aacaacatca
    11701 gccagacaaa gacgttatcg accgccagcg atttctcaat cagataaccc gtgagaaagg
    11761 ccagggcctg tggatcggca acggcgcgac cttcggtttg caccagatac caccagaaag
    11821 cggcgttaaa cagtaacgac agcgtcaccc agaccagcga ccaggccgca gcctgtttca
    11881 tggtcatggc atgtgcccca cgacgcccct gcaacaacag gtcgatagcc agcataatgg
    11941 cgacaacaac agcgaatccg ccccatagca acggcgtgcc gacagtattc atagaagttc
    12001 cttacacata aaaaaacggc caacgtcgga agacgtcagc cgctgctttt tatgcataga
    12061 cctcgccttc cggcaaggtc tcacttacaa caaaaaagga atacgtctgc gtattccttt
    12121 cgggttgccc ggtgaccgga tgtggttttt cacacatcgt aatgacgatc gaccggcaat
    12181 gaagttactc ccctttgcag gtaacaaagt atgacagacc attcagtcag tcaatggtct
    12241 gttacatacc ttttacactg ttttactttg gcaagtatag ttttacgcta tctgccggaa
    12301 aaatcacccc agtttggcgg cggatctcgg tcagcagttt ggctgtgatg cgactgaccg
    12361 ccagccctgg atgatccacc agatgctcat ccaccagggt agcgaacagc tctgcttcgt
    12421 agagcatggt attaatatgc tgcggctggg tgagatcctg catctggctg ccacgcggca
    12481 caaagcagac tttctggcat tcagacagtt tttcaatcac cagcgatccc gcttcgccct
    12541 gaatctcgct tcccaggaca gaatcactga cctttgagtg ctgcaaggtg acgctgaaat
    12601 caccgtaatc catcaccacc acgccgtggg cgtcaacgcc gcttgccagc aaactcgccg
    12661 ttgcctgcac gcttttcggc tcaccaaata acgccaccgc cgacgccagg cagtaaaagc
    12721 cgatatccat aattgaaccg ttagagaatg ccggattaaa ggtgttggga ttctcaccgt
    12781 ccaggtaacg ttgataccgc gaggaatact ggcaatagtt gaaaaagact ttacgcagtt
    12841 tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt cggcaggcag gcggttttaa
    12901 atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc aatggcggca tccacttccg
    12961 ccagattcga cgccagcggt ttttcgcaaa tcacatgaat tttatggcta aggaaaagtt
    13021 gtgtctggga aaagtgcagg gaattcgggc tggcaatata caccgcgtca atggcatcgc
    13081 tttccgccat cgcttccagc gacgtaaaca gatgctcgac agaaaaatca ttggcgaagt
    13141 gctgagcctg ctcaaggctg tgggaatata cggcggttaa cttgtattta ccgctctcat
    13201 gggcggcctc gacgaactgg cgagtgatcc agttcgtacc aatcacagcg aaacgtatca
    13261 taacgcgtgg atactccata aagggattct gtcgtcaatg tagcacgcct ttttcatctt
    13321 cgacgtgcgc agatacgcat tattctttcg ccggacagcc atgctcacta aacggcccaa
    13381 tcatgaccga tggcccgaga tatttcggct ggcgatggag gatttttgcg tcaatcaccg
    13441 cgctgacacg agcgagtttc tcccgcagtt gagtataaaa accgtgcttc atattgaggt
    13501 ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt tgccagccag atggcgcgtt
    13561 ggttatgaaa ttcttgcgag ataatggtga tatggttttc cccaaacacc tttttggcac
    13621 gcactaccga atccagcgtt gagaatccgg catagtcgca aaagatcact ttcgccggta
    13681 cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc atcgtaattt ttacgtccgt
    13741 tatcgccact caccagtagc catttcactt ttccggcgtg gtacaacgct gctgcggtat
    13801 caatacgtcg ggtaaagtag cgattacccg gcctcgcccc cagcaataaa cccacgttac
    13861 gcgccgggac ggcgttgaca tcgccccagg tcagctgctt gctggcgttg accataacgc
    13921 gatcggcgat aaaaatggtc gctcctgcca caagtaacag taagcaggca atctttagag
    13981 tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta
    14041 tccttaacga aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga
    14101 catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg
    14161 aactcaagct ggtggtactg cggctccata tggcagcaca attgatagaa cgctttgtta
    14221 tgctctttct ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc
    14281 ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgccgccc
    14341 tgcacacgag aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg
    14401 tcatacatca ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga
    14461 gtatattgcc agagagcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc
    14521 agcacatcgc ccagacgctg ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg
    14581 ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt
    14641 atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga
    14701 gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg
    14761 ttaacccgct acaggcgtgg gaagcggcag atgaatattt gctgcaacag ttggacgaca
    14821 cagaaatccc cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc
    14881 tggcggaaca taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg
    14941 agaatttacg cctaaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg
    15001 actacccgca acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg
    15061 aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg
    15121 ccaaagcccg tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccga
    15181 ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc
    15241 cgctggccga tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc
    15301 ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc
    15361 aacatctgcc agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta
    15421 ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc
    15481 cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc
    15541 gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg
    15601 tgctctgcaa ccctccgttt caccaacaac atgcgctgac cgataacgtc gcctgggaga
    15661 tgttccacta cgcccgccgc tgcctgaaaa tcaacggtga gctgtatatc gttgccaacc
    15721 gccatctgga ttacttccat aaactgaaga aaattttcgg caactgcacc actattgcga
    15781 cgaataataa atttgtggtg ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg
    15841 agttacctgc cggatgcggc gtaaacgcct tatccggcct acgttcagca cgttaccccc
    15901 aggccggata agacgcggca agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc
    15961 gcttccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt
    16021 ctacgtttat tcttccgtca cacagataaa ttcatccgtt gccaatctgt caacggatgt
    16081 tatcatattg ccaatttgtc aacggacgtg atgaagaatg cacctgataa ctcaaaaagc
    16141 attgaaagat gctgcggaaa aatacccgca acataaaacg gagttggtgg ctctggggaa
    16201 cacgattgct aagggatatt tcaaaaaacc tgagtcatta aaagcagtat tcccatctct
    16261 ggataacttc aaatatctgg ataagcatta tgttttcaat gttgggggca atgaattacg
    16321 tgttgtagca atggtctttt ttgaatcgca aaagtgctac atacgtgaag ttatgacgca
    16381 taaagaatac gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt
    16441 gccgacatct tgcaagcagg agaaaagcta actgctgtgg caccttttct ggcgggtatt
    16501 cagaacgagg aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat
    16561 cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg
    16621 cccgaatttg cggaatttaa tgccatggct caagccatgc ctggcggtat agccgtgatt
    16681 cgtaccctta tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa
    16741 tctatggtgt cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa
    16801 ttggcaacgc gattcggcat ttctcccgcc ttgtttattg attaacgcga cgtgccggat
    16861 gcgttgcatc cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg
    16921 gcaattgccc gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca
    16981 gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag
    17041 atcaccacat tgtccactgc taatacctgc gtttcgccgt tgatcaccac atgcagcccg
    17101 tcatcgtcaa tcttctgata acttacgcct gggatcattt tcacaccccg cgagagcagg
    17161 atggtgcgat ggatccagcc ggtggttttg cctaacccct gtcccggttt gctggctttg
    17221 cgctggagca tcacaatttg ccgtgggcta cgcgggatct gcattccctg cgggcttaag
    17281 ccaccagcct gttgtaggct gctgtcgatc ccccactcat tacagaacct ggcgatattc
    17341 tggctggtgg attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca
    17401 caaccgatga tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac atcgagataa
    17461 ctcaatacct tcggatgatc gatcccgtcg ataggcggaa cacgcggcac gatcccactg
    17521 gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg attgagtttc
    17581 agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc
    17641 tctttgccgg ggatctgttt ggcgatatta aactgcccgc cgatctcgct gtgcgcatca
    17701 aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt
    17761 ccagcgccga ccaccgccag attttttttc tgcacggcgg gaaggattgg cattttggtt
    17821 tcgtggcagg cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg
    17881 aggcagccct ggttgcagcc aatacaagtg ttgatctcat cggctcgccc tgattgcgct
    17941 tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg
    18001 ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa tccggttggt ggttaccagc
    18061 ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg
    18121 ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta ttccggtgtt gataatggtc
    18181 gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct
    18241 tcgaccaggt cgagcatcga caaccggtaa ataataatga agtcgttgcc gacgcgttcg
    18301 cgcaccgtac gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac
    18361 tggtcactac gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcagag
    18421 cccatcactt cgacgccatc gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa
    18481 tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg
    18541 ggggcctgca atgcggacgg tgcgaccaga tgcggttggt agctgtagcg cccggtatgc
    18601 aaaatttgca gggcgatttt gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg
    18661 tgtgggatct ggctggcgtc gttgagcatt gcgctacctt ccatgccaac gcctgttaaa
    18721 tctggtgcaa tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa
    18781 aacgcagcca gccgttcagc accgtccggg tattcctcca gcccggtgtg cattgaaccc
    18841 atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg
    18901 tagctcataa aattgtccag tatgtaaaat aattgttatg tagtcggatg agttctaatt
    18961 tagccgtcga tggagaaaag ggaaaagggg agtgcggggt ttgtgatggg attcaaaaag
    19021 aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg ttttatccgg cttgggggat
    19081 tgcacatgta gggcggataa ggcgttcacg ccgcatccgc cagccgttgc aaattactgc
    19141 ttacggaaaa aatcgttata caacatatac aaatgagcgg cactccagga gaaatttggt
    19201 gcgccttgct gcgcgcctgt cagcgggttg taattctcct gaattgggcc atcggcggtt
    19261 aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca gggcatcatc acgataaccg
    19321 taacgctcca tccctttcag accaaaccag aactgatcca cccatacgcg cccgcgccag
    19381 tagatatcag cgccaaaagc cggattggtt aacgccgccg ttcccagcgg aacaaaggtg
    19441 ttgaactctt taggatcgag catcaccttc accaccgcgt cggcattggc ctgcgttgcc
    19501 gcaccgttaa acagcggcga ccagccttcc ggccctttac cgcgctcaac aatcggtttg
    19561 cccgcgcagc cgttcgccag tggtttatct tcaatacgta cgtcatagta gaactgggta
    19621 gtcgggtcga acatacaggt gttgatgtag tccgcgagct gctgtgccaa ctggcgatag
    19681 cgtttggcct cttctggctt accgaggatg gttgccatct ccgccagata atggttatcg
    19741 ctgtacatat agctggcctg atccaccgac tcctgcaata gcgagtagcc cagcaacgtt
    19801 ccgtcctgac tgcggttttc ggcgaatttc accgtccagt cgctacgttt gccgccatta
    19861 gcgacatatt tatccaactg ttctttgtcg ataaaaccaa agacggcggc atcatcacgg
    19921 cccgattccc acgacgcagc gacctgtgcc ggaatttcca gactgtcgta ctggcctttc
    19981 tccaccacgc gggcgtagtt gttcagccca gactgcgtct cttctttatc gccttttttc
    20041 accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt tgtcgcgggt cgcgccatat
    20101 tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc accagtcgtg ataggccacc
    20161 agtttcgggt acatctctgc caaccaggct ttatcctggg tgacgttgta tacttccatc
    20221 accgaccagg cggcaaggct gggtttggta ttacgttcgt tccagttgcc gccatcgcca
    20281 ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga caaagcccac atcctgcgga
    20341 cgcacgctgt cgccaggctg gatctgccag gagaagaccg cgcggatatt ctctttggcg
    20401 atatccggat tgaaatgcgc catcgcaaac gcctgcttcc aggtatccca cggccaggtc
    20461 tgattgccgg agaaccagcg tccggtcacc gacggcgtga cggtgttata attcaccgca
    20521 ccgcccggtg agcgccagtt accgttgagc gtttcgatcg ctttcaccgc gacgcgcgtc
    20581 tgttccggcg tcgcatccgg attggttaac cctttcttca ggtattcttc ccagcgttgc
    20641 tgcgaggcgg tgagataaaa cgccggacgc gccagaatat cgcggatctg catttgctct
    20701 ttgctaactt cctgagcggt cagcagatgg gaataggtgg tgtagagcgt ggtcgaaccg
    20761 ttgatatgcg ccttactggt aaagcgatta ccgttgattt cagtctgcac cggcagggat
    20821 ttatgcacct gatattccga ttcgccggag gtcagcagat cccaggtggc gcgcactttg
    20881 ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt tgcgctgata gtcagggtat
    20941 tcgccggcaa tggttttatc ggaaagcggt ttcccttctt tcgcttccag tttttccagc
    21001 agttcgccat cccacaccag atccagcggt ttattgctga tgattttagt ttccagtagt
    21061 gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct gcacatcttt tgctgtcagt
    21121 ttttgcacca gcgcgccggg aatactgtat gcctccagcg tgaagttgac tttcttgccg
    21181 tcttgccaga cagtcaggcg gtcgaaattg ctggccataa agttgatgta ttcttccgtc
    21241 agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc cgtctggcaa cagatgacca
    21301 tgccaggctc cgagatcgaa aaacggatta aaacgctggt gatcgtcgta atcgtaatcc
    21361 ttcatgtact gcggcgcacc agtacggtta atcacgtttt tataattgtc ggcgttagcg
    21421 gcatgagcgg aaaaacttat cagcagagcg caggttactg gcgttaaaat agttttgatt
    21481 ttcatgagat tacagtcctt attaccagta gaaatattgc atcaggaaga tttggtcgct
    21541 gggatcgtta gaggcatttt tcatgtagaa gcggctatca aaagccgtgg cgaaacgtcc
    21601 gccgccatat tcgtaccaga taccaaactg catgaaagag gtggtttcat cgtcagcgtg
    21661 gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga taaacgcctt ttaacgcttc
    21721 atacatggcg ctcaccatca tcccgctttc acttcccggt acgtccagcc cgtcggcacg
    21781 tccggtatgg tgccaggcgc gagcggcaaa gttaggggta aacaggcccg tgagatagag
    21841 ttgtcctgtg ccgtcggtaa tttccagacc attcatccag gtgatgcctt cggcaaggtc
    21901 atactggatg taaccgttca tccattgccg gataggtgta tttgtcgtcg tagcgatcgt
    21961 acttgccaaa atgtagccag gctttgcttt cgtcatgatg tccggcaggt gtggcggtga
    22021 cgctataacg aaagttgccg cttaagttct gtaccttcag cgcggtcata atgtcgcggg
    22081 tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc ccaccattgc aggtcatcca
    22141 gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc atcattggta cgataaccgc
    22201 catagaaacg gttaatccca ccttcgaaac caccccagct atggtcaggc acctaggcat
    22261 taccgtcgtg gtcggcctgc acggtccagc cttcgccgta aatcaggccg aaccaggagc
    22321 cgtacttcat ctccaggcca ccttcaatat aggtgccgtc gctgtattta tgtttattct
    22381 caccctctaa atccatatat ccaccaagcc ctaattcgcc gtaaaaacga aatgaaggag
    22441 aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg ttctgccgga cgtgcttctt
    22501 ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag taatgctctg catggtgcag
    22561 tgattaattt cattgtgtta atccatgagc taaagaaaaa gtacagcctt ttatcgttgt
    22621 aattataaaa atataaaggc agaaactatt ttccctctcc tcatccagga agagggaaaa
    22681 tgcctgctca ttttttatta gtctgcttaa tcaacattgc tggctggcgt cgcttcaata
    22741 tggcgctctt cagccgttaa tccctttata tatttgctgt atttccacca cgcaaagccg
    22801 aggaagataa caataccgcc gacgttataa aaaatgatgg tcagaatatt cgcgccagtc
    22861 gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa tcagcattga aacaacgata
    22921 ataccggtgc ggcgggagcc catacggaaa tcgcgtggca ggtgatctaa tttggcgcgt
    22981 aaattcaggt aagccagcat gatgaataac ggcggaagca tggacgctgc ggcggtcata
    23041 ttaataatgg tattcatcag atcctgcacg gtattggaac cgagcatcgg gataatcatc
    23101 agcgggatga cgatcagaaa ctggatccat gctgcgcgcg ccggaacgcc gttttcgtta
    23161 agttcgacgg ttttcttacc aaagatgcct tccgggattt cggagaagaa aattttcacc
    23221 ggcgttgcgg tccacatcag cagggaaccg aacattgcgg taaaggacac cagaccgaca
    23281 aagcgattca tcaacgcttc cggcagacca aaatacgccg ccatgccgtg gaatacctgc
    23341 accgatccgc cggtaaattt caactctttg ctgctgacga agacgttaat cagcaccgag
    23401 gagacggaat acagtacgcc gataaaaatc ccggcgagga tgatcacttt aacgaacgat
    23461 ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg actccgcgcc acctgcggcc
    23521 ataaagatcc aggtggtaac gccgaggaac gcccagttga agttcgggat catcgcgtca
    23581 acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg ccgtacctgc cagtaaaatg
    23641 taggagagcg ttaacagcag catcagcgtt gaagtgacgg aggtaattgg ccccagcatt
    23701 ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca gcaccatact gataacggtg
    23761 gtggcaaccg gcgtcataat atattcgtag ccgaggaagg catacgaagc ataagcaata
    23821 acgcgcggca acagcgaggt gaaaaagaac agatttacga accagtaagt gtaggcagta
    23881 ataaatgccc aatgtccgcc cagcgaactt tttacccacg cgtagacacc ggcttctgag
    23941 tttttattta acgaaacaaa ttctgcgatg atcagacaga agggaataaa ataaaaaatc
    24001 gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct caatattatt attgataacg
    24061 ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca agccgaattt gccgattgta
    24121 ttacgtttgg tatcagacat aacacatctc cgaaaatgtg tagggtagcg gtggcggcaa
    24181 actccttttg ccgccgtagt tgttatttgt tatggaaata accatcttcg atggtgactt
    24241 tgagaaccac ttttttgacc gcgttattgc agataaaccg atacgcttca tggatatcgc
    24301 aaataacgat ttgcccttcg tggacctcta cggtttctcc gcagcctttt aaatattcac
    24361 ggtcagtttc atcacgataa tattccacta cctgtaatgt ctctttcggc gcatattcaa
    24421 ttttttgctg cccttgcagg tagtaatgca cttcaaaata gcgtcgatgc ccggtaaata
    24481 gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa tgaatcaccg atggagtggg
    24541 cgacgccagg ctgaatgttg tcgatatttt caatcgcttc aacgcagcgt tgccacttct
    24601 tgccagaggc gtaaatctgg cggaactgtt ctaagttatc gatgatcctc attgcttatt
    24661 ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac tcatacgacg ccaggctttg
    24721 cgcggtagct tctccgccag aaaccggcag caacgtaaag ccgtagctga agtcacggaa
    24781 ccagacgcgc caggagtcca gcacctcgct gccccaggag ttggagccga ggccaagtag
    24841 ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc agctcgttac agtgctgggc
    24901 agcgtggatg ttttcctggg tatagtgcca ggcgctgaag ttaattgggc gctgcggaac
    24961 caccagcaga ccgttgccgt ggcggttagt cagtgccgtc cagcggacat gctgacggtt
    25021 gccgttgttc tgcgggaagg gatagttctc gaacatggca tcgacggtgc tgcgccagat
    25081 atcgatgatg ttagcctgct ggctgtcggc gtagttttcg cccggtccac gaccgtaata
    25141 cgccacctga tcgtattcgc cgttaatccc catggtgaaa ccgatgcacg gaatgatgtg
    25201 cggatagtcg ccgtaacgct cgccggaaag cgccacgtta acctggccat cggcagcgat
    25261 gcgccagatg taggtacagc gcatcccgaa gtcaaacacc ggcggtgcaa taactgtgcg
    25321 gctgatgatc aacacttcac catcgctctg ttctacggca aagtcgcgca gatgttcctg
    25381 catgatctgc aaatgattcg gctgccacaa cccttcgtac tcctgcttgt ggttgtcgat
    25441 catcggcttg aagaagttga tctttggctc gcgggtcagc agcgactcgc catttacctg
    25501 gcaggatgtc ggtttgccac tcatttttga gaaggtgatc gcgaagttgt agccgcgaac
    25561 ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc gcattatttg gtgcgaaagg
    25621 cactggctgc gcggtgtttt ccttcagcgg gaactgataa gtggcgatag aatgcccggc
    25681 ttcgctgtag cgggtgcggg aatctttggt caccgtaatg ttgaggaacg cttcgcgggc
    25741 gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg ttcggcgcaa cgtcgcgcag
    25801 tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc acctctgcgt gcagggtgta
    25861 gtcatcaagc gtggtaaacc acagtttatt ttcgactttc aactcgccgc gagtcagcgc
    25921 gtggattttt accggcgcga taacctgttt gtactctttc aggcccgatc ccggcgtctg
    25981 atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt cgccgtagtc
    26041 gccgccgaat ttataccaga cattgccgtg gtcgtcctgt gcctggatcc cgtggtcgca
    26101 ccactcccag acataatgac cctgaatgca atcgtgctta tagaagacgt tctggtactc
    26161 cgtcagcccg cccggtccgt tccccatcgc atgagcatat tcacagatga tgcgcggctt
    26221 cggatgcggg tattcaccaa actcattcat cagcggcacg cgggtgtaca tggtggaaat
    26281 aatatcgacc acttcagcat cgcgatcttc ttcgtaatga accagtcgcg tgtcatccag
    26341 cgctttcgcc gcatggtaca tcgcgcggat gttacagcca tagccggatt cattgcccag
    26401 cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg atatggcgaa caatgcgctc
    26461 gacgtagact ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc cgacattagc
    26521 aaagccgtgc gattcgacgt cggtttccgc catcacaaac aggccgtaga tatcacacag
    26581 ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc acggagttga tattgtgctg
    26641 cttcatcaac tggagatctt tctcgacgcg atccattcca acggcgcggc ctttgcgatg
    26701 atcgttgtcg tgacggttga cgccgtgcag catcacataa cggttattga tccagaacag
    26761 accgtcgcgc actttgatat cacggaagcc aacgcgttgt ggcaccactt ccagaacgtt
    26821 gccgttggcg tctttcagcg tcatgaccag atggtaaaga taaggggatt ctgctgacca
    26881 ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg gtcagttttt caattgccaa
    26941 atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca aacagggtat attccagcgt
    27001 cgtgacgaca ggggaggcgg cgagattttc cagcaccact tcgcaggaaa gcgtggcatc
    27061 gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg ttaggtgttt
    27121 tccgaccaga taaacatcgc ggaagatccc cgccgaccac cacatatcct ggtcttccac
    27181 gtaggtagag tccgcccact gcatcatgcg cacacacaac aggttgtcgc cggttttaac
    27241 catcgcgctg atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac ccacatactg
    27301 accgttaacg tagacttcaa aataggtttc gacgccgtca aatttaatca gcgtctgttt
    27361 accctgccag ccgtcgctga gggtgaaaat acgttgatag gcaccggttg ggttatcgct
    27421 ggggacaaac ggcacatcga tggggaacgg aaaaccttcg tcggtatatt gcagtttgcc
    27481 gtgaccttcc atttgccaca tggcggggac ggtaatatgc ccccagtcag ccattaactc
    27541 agaggtgaag gcttctggta cttgcagcgg atggtcaaaa aagtggaaat tccactgact
    27601 gcttaagggc agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt gcgcaacaga
    27661 atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga gctgaatgtt
    27721 ttcccagcga ttcatagggc ctcccggtag agagaaatcg gaaccattca tggtgatggt
    27781 agtttgtgta attgctgggg ggttagtaaa atttttacta aaaagcagca ggtaaatgca
    27841 tttactttaa ctggatcacg aaaataaacg gcagcactgt ccggcaaaaa tgattgccgg
    27901 acagtgtgta aggggattta acgggtcgtg ccgcgcagtt ttaatttgct gggaacgaag
    27961 actagcagcg gcagcgcgcg accatcgcgg gctttttcat acaccaggtt aacgccctga
    28021 cttcccatca tctcggaatg gatgcgcacg gtggagagcg gcggaaaggt aaatcgcgcg
    28081 gtggggatat cgttaacgct gataagcgaa atatcttgtg ggatgttcag gccacgttca
    28141 tgaattgccc gcagtacgcc gatagcaatg gaatcggaag caacaaacag tgccttcgga
    28201 tagtcttccc gcgccagcat ttgttttgcc agttcataac ccgacgaact ggaaaaaccg
    28261 ccgcgccaaa tatcctcttc gcgtaccact tgtttcagtc ggccatattc cgcaaaggcg
    28321 acctcacgaa tatccgcctt gccaggctca tcttcaccgc caataaaacc aatacgatta
    28381 acgccctggt tgatatagaa gtcgatgatt tctttactga tgcgtgccag atcgatatcc
    28441 accgcatcgt aaccgctgcc gggttcgtga aagtcgataa aacagatatt gtcggtcaac
    28501 gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga caattaaaat accggtgacg
    28561 tttttaatgt ctggtaatcc gttgtgttca taacagttgg tcagttcgat cgccagcttt
    28621 tcgcactgag tttcaatgcc gtggcggatc gccagatagt aaggatcgtt gatctccagc
    28681 tcctgctggt agctgtagat agccagaata tggtgttggt tgactgcgcc tgtctgtagt
    28741 ttacgggcgc tactggtctt gtactccagc ttttcggcga tctcgagaat gcgatgtttc
    28801 gtctcttctt tcacattcaa tgtcggatcg tcatttaaga ccctggatac tgtcgccagg
    28861 gatacgccag cttcgattgc gatgtctttt aatgttgcca tgtttacctt ttttactttc
    28921 cctgatgtgc gcccccattg tagaggaagt gcttatcggg tcacctgttt tcagtaaata
    28981 aagcgcgttt ctttgacgct gtaacggtaa cacagcggct taggaatgtg atcacgcctg
    29041 cattctccat ttttagccta caggattgag aaaaactttt ctatagttta gtaaaatttt
    29101 acccgaaggg aggaagcaat ggaaaccgtg gcttacgctg attttgcacg tctggaaatg
    29161 cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg ccgacaagct gtacatcgta
    29221 caggttgatg tggggaaaaa acactgcaaa ccgtgaccag cctggtgccg tactacagcg
    29281 aagaggagct gatggggaaa acggtggtgg tcttatgcaa tctgcaaaaa gcgaaaatgc
    29341 gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac cgatgacggc agtgaaagcg
    29401 tgttgttaac gccggagcgg atgatgcctg cgggcgtacg cgtcgtataa aaagatcgga
    29461 tggcgacgtc gtatcgccat ccgatttgat attacgcttc ttcgacactt actcgcatgg
    29521 ccgccagcgc cttacgcgcc gctttgatca ccagttcaca ctgttcaatg gtcagtgtca
    29581 gtggcggttc aatgcggatc gttttggcgt tattgagcgt tccggccacc agtacgcgct
    29641 ggcggaacat ctcgctggca aagttatagc cgatttcgtt atcaacaaac tcaatcgcca
    29701 tcaacatccc tttaccacgc gcttcctgta ccagatcggg atattcccgc gccagttgac
    29761 ggaaaccgtc cagcaacata tcgccttttt gctcagcctg agccggtaag ttctgctcca
    29821 gcaacacatt gatggtcgcc agcgccgccg cacaggccag cgggttgccg ccaaaggtgg
    29881 tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac ctcttcagtg gcgatggtcg
    29941 cgccaatcgg catcacgccg ccgccgaggg cttttgccag acacagaatg tccggctgaa
    30001 cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc catgcccgtt tgtacttcat
    30061 cgaggatcat cagtgcgccg aactcatcgc acagcttacg cacggcggtg agatagcccg
    30121 gcggcggcag aattacgcca ccttcgccct gaatcggttc gaggatcacc gcagccacat
    30181 catcaccggt ttttttgcac tcattaagag ccgtgcgcat ggcttcgata ttgccaaacg
    30241 gcacatgacg gaaccctggc agcagtggca taaacggttt gcggaaggtc gatttcgccg
    30301 tggccgacag cgcgccaagt gatttaccgt ggaacgcgcc gctggtggca ataaaagtaa
    30361 atttgccgcg cggcgactga taagccttcg ccagcttcag cgccgcttca acggactcgg
    30421 tgccgctatt acagaagaag ctgtatttca gtttaccggg cgttagcgca gcaagggttt
    30481 tcgccagcat agcccgtaac ggatcgagta gctcctggct gtgcagtggt tgtttcgcaa
    30541 gttgattctg tacggcggaa accacaactg gattacggtg ccccacgttg aaaattccaa
    30601 aacctcccag gcagtcgata aactcctgtc cctgggtgtc gacaagcgta tttaaacctc
    30661 ccgcttgcca ctctacggct ccgtaatccc cgccggcggt aacagatttg cgatactcta
    30721 aaaaccccgg attgacatgc tctttgaagt attcaatcac ctctcggtta agtgctttca
    30781 tctcctcatg atccagcgtt cgcttctcaa tgagattcag ggcgtgggcg ctgcacgcta
    30841 aagccgatgc gctcgaaggt aacctgttca aaatatgctc cggaggctcg cgtatcacat
    30901 gataccgatt taagtattgc agggattgcg ccactccggc tgcgatcgcg aaaatcggga
    30961 taaacacaaa tgcggttatc atttgtgcaa catttaatgc tatccgttaa catttgttgt
    31021 tattttgcac ggtttttgtc gggtatggat ttgatgattg ctgcgcaaat gcacgttttc
    31081 agggcaataa ttgcctgatt tttgggcaag ataattaacg ttttcgtata gtaacagagt
    31141 gaaagtattt taaatcaaag aattagattt gtgctttaat cgcaaattgc gatctaaatc
    31201 aaattaatcg gttaaagata accgcagcgg ggccgacata aactctgaca agaagttaac
    31261 aaccatataa cctgcacagg acgcgaacat gtcttctcat ccgtatgtca cccagcaaaa
    31321 taccccgctg gcggacgata ccactctgat gtccactacc gatctgcaaa gctatatcac
    31381 tcatgctaat gacacttttg tgcaggtgag cggctttacc ttgcaagagt tacaagggca
    31441 gccgcacaac atggtgcgtc acccggatat gccaaaagcg gcgtttgcgg atatgtggtt
    31501 caccctgaaa aaaggggagc cctggagcgg catcgtgaaa aatcgccgca aaaatggcga
    31561 ccattattgg gtgcgggcca atgcggtacc gatggtgcgc gagggaaaaa tcagtggcta
    31621 tatgtcgatt cgtacccggg cgacggatga agagattgcg gcggtggagc cgctgtacaa
    31681 agcgttgaac tccggacgta ccagtaagcg tattcataaa ggcctggtgg tgcgtaaagg
    31741 ctggctgggt aaactgcctt cattaccgct tcgctggcgg acgcgtggag tgatgaccct
    31801 gatgtttatc ttgctggcgg ccatgctttg gtttgttgct gccccggtgg tgacgtattt
    31861 cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt gaatggcaga ttgtgcgccc
    31921 gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc ggagagcgta atagtgttga
    31981 gcacctgaat cgcagcgatg agctggggct gacattacgc gcggtagggc agcttggcct
    32041 gatgtgccgt tggctaatta acgatgtctc aagccaggtg tccagtgtca gaaatggcag
    32101 tgagacgctg gcgaaaggca ccgatgaact gaacgaacat acccagcaga cagttgataa
    32161 cgttcagcaa acggtggcga ccatgaacca aatggcggcg tcggtgaaac agaactctgc
    32221 cacggcgtcg gctgccgata aactttctat caccgccagt aatgcggcag tgcagggtgg
    32281 ggaggcgatg accacggtga tcaagacaat ggacgatatc gccgacagta cctagcgcat
    32341 tggcaccatt acttcgctga ttaacgatat tgcgtttcag accaatattc tggccctgaa
    32401 tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa ggttttgcag tggtggcagg
    32461 ggaagtgcgt catttagcca gccgcagcgc taatgctgcc aacgatattc gcaagctgat
    32521 tgatgccagt gctgataagg tgcaatccgg ttcgcagcag gtacacgccg ccggacggac
    32581 gatggaagat attgtggcac aggtgaaaaa cgtcacccag ttgatcgcct agattagcca
    32641 ttcaacgctg gaacaggccg atgggctttc cagcctgacc cgtgcagtgg atgagcttaa
    32701 cctgatcacc cagaaaaatg ccgagctggt ggaagagagt gcgcaggtgt cggcgatggt
    32761 gaaacaccgc gccagccgac tggaagacgc ggtgacggtg ctgcattaat cggtatgccg
    32821 gatccggcgg ttggagcaca atgcctgatg cgatgctggc gcatcttatc aggcctgcgg
    32881 ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg tcgcatccgg caattgcacc
    32941 gcgccactgg cggatgcggc gattaatcca gttgcgtaat atcaaaagcg gcgcggtcga
    33001 tcaccgcaat gatctttttt atttgtgcat cactgatatc gctctggttg acgcgtaaat
    33061 ccagcaccgc tttaaaatta tccagcgccc gcttcatttg cgggttctgg cgcagcgccg
    33121 cgccaacgca acgcgctttg atgcgttctt caatcatctc cacttgttcg cggttttctt
    33181 ccagccactg cgcgccttgt tcggtcagcg caatctgctt cttaccacct tcctcttcgc
    33241 ggatggtaat cagcgactgc tcctgcagaa aatccagcgt cgggtagatg acgcccgggc
    33301 ttggggtgta attcccctgg gttagattct caatcgcttt aatcaattcg taaccgtggc
    33361 tgtcatcgcg cgagagaata tccagaatca ccagacgcaa ttcaccgtga ccaaagaaac
    33421 gttgccgacg accgccgccg cgaccgtggc gaccaccgca acattgaccg tgttcatgct
    33481 ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc tttgcagcag ccctcatggc
    33541 gtggctggcc ttcatgttta caacaccctt cgtgatgatg gctcatatca gcctcctttt
    33601 gcttgattag atatatctaa tttatatctg atgtgagcat tactgcaagt acaaaaatga
    33661 tttttaaacg tggaagcacc tcacatctag gccggataag acgttcacgc cgcatccggc
    33721 agcattgcta tggcactgaa tgccttcaca tacttcagac atatcagttc tacaaatcgc
    33781 ttgcatttat catgattaac aatcattatc atttgcgaat tttatttaga tatatctgat
    33841 taccatcacg aaggcgataa caatgaataa caccccccgc tacccgcagc gcgttcgcaa
    33901 tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag cgcatcagcg ccggttttca
    33961 gcgcattgtc cttggcggcg aggcgttgga cggttttaca tcgcgcggct ttgacgatca
    34021 cagcaaactc ttctttcctc aacctgacgc tcactttgtg ccgccaacgg taacggaaga
    34081 gagcatcgtc tggccggaag gaccacgccc accgtcgcgt gactatacgc cactgtatga
    34141 cgaactacgc catgaactgg cgatcgattt ctttattcac gacggtgggg tcgccagcgg
    34201 ctgggcgata aacttacggt ggcaggtccg cgcggttcgc tggtggtgcc ggaagattac
    34261 gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg cactgcgccg ccgcctggaa
    34321 acgttgagca aacttgccgt taaaccgcaa gttagtgcgc tggttagcgt gcgggataac
    34381 gcctgtcagg attatctcgc gcaccttgat ggttttaata tcgaatggct ggcacatgat
    34441 gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc ctgccgatga ttacttcatc
    34501 tggataaccg gcgaaggtaa agtcgttaag aatttaagcc gccgctttga agcggaacag
    34561 tatgacccac agcgggtacg tgcagcggct tactggcacg caaagtaact gatatataaa
    34621 aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat ctggtggccc ctgctggact
    34681 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aggggccatg
    34741 gtagcggatt ataaagtaac tccgtgtcgc aatccagcca ttaccgcgcg cctgctgttt
    34801 ttataaacaa tgtattttca atccgttata cttttcctgg tgatgttaga aaggagtaaa
    34861 tatggttgag gatattttgg ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg
    34921 gctttcatcc gccgggactg gttttccctt tgctcatccg gcaaatcgct tctggaaggt
    34981 gatatatcag gccgggttta ccgaccgtca gttgaagccg caggaggcac agcatctgct
    35041 ggattatcgt tgtggcatca ccaaactggt agaccgtcca acggtgcaag ccaatgaagt
    35101 ttcaaagcag gagctgcacg caggtgggcg taagctgatt gaaaaaattg aagattatca
    35161 gccgcaggcg ttggcgattc tgggcaaaca agcatatgaa cagggattca gctagcgcgg
    35221 tgcacagtgg ggg
//
LOCUS       DAJTRB010000034        34985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTRB010000034 DAJTRB010000000
VERSION     DAJTRB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.034"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..805)
                     /locus_tag="OO330_001791"
     CDS             complement(<1..805)
                     /locus_tag="OO330_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaH family type III secretion system E3
                     ubiquitin--protein ligase"
                     /protein_id="HCR5882682.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
     gene            897..983
                     /locus_tag="OO330_001792"
                     /pseudo
     CDS             897..983
                     /locus_tag="OO330_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1388..1864)
                     /locus_tag="OO330_001793"
     CDS             complement(1388..1864)
                     /locus_tag="OO330_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5882683.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1923..3212)
                     /gene="bioA"
                     /locus_tag="OO330_001794"
     CDS             complement(1923..3212)
                     /gene="bioA"
                     /locus_tag="OO330_001794"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5882684.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QMAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3299..4339
                     /gene="bioB"
                     /locus_tag="OO330_001795"
     CDS             3299..4339
                     /gene="bioB"
                     /locus_tag="OO330_001795"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5882685.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4336..5490
                     /gene="bioF"
                     /locus_tag="OO330_001796"
     CDS             4336..5490
                     /gene="bioF"
                     /locus_tag="OO330_001796"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5882686.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5477..6232
                     /gene="bioC"
                     /locus_tag="OO330_001797"
     CDS             5477..6232
                     /gene="bioC"
                     /locus_tag="OO330_001797"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5882687.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6225..6902
                     /gene="bioD"
                     /locus_tag="OO330_001798"
     CDS             6225..6902
                     /gene="bioD"
                     /locus_tag="OO330_001798"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5882688.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="OO330_001799"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="OO330_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5882689.1"
                     /translation="MSKPFKLNSAFKPSGDQSEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="OO330_001800"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="OO330_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5882690.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="OO330_001801"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="OO330_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5882691.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="OO330_001802"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="OO330_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5882692.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="OO330_001803"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="OO330_001803"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5882693.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="OO330_001804"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="OO330_001804"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5882694.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="OO330_001805"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="OO330_001805"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5882695.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13839..14543
                     /locus_tag="OO330_001806"
     CDS             13839..14543
                     /locus_tag="OO330_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5882696.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14748..15461
                     /locus_tag="OO330_001807"
     CDS             14748..15461
                     /locus_tag="OO330_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5882697.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15497..16453)
                     /locus_tag="OO330_001808"
     CDS             complement(15497..16453)
                     /locus_tag="OO330_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5882698.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16453..17694)
                     /gene="clsB"
                     /locus_tag="OO330_001809"
     CDS             complement(16453..17694)
                     /gene="clsB"
                     /locus_tag="OO330_001809"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5882699.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17691..18452)
                     /locus_tag="OO330_001810"
     CDS             complement(17691..18452)
                     /locus_tag="OO330_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5882700.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18585..18995
                     /locus_tag="OO330_001811"
     CDS             18585..18995
                     /locus_tag="OO330_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5882701.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18957..20063)
                     /locus_tag="OO330_001812"
     CDS             complement(18957..20063)
                     /locus_tag="OO330_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5882702.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20074..21207)
                     /locus_tag="OO330_001813"
     CDS             complement(20074..21207)
                     /locus_tag="OO330_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5882703.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21200..22936)
                     /locus_tag="OO330_001814"
     CDS             complement(21200..22936)
                     /locus_tag="OO330_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5882704.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22929..23924)
                     /gene="hlyD"
                     /locus_tag="OO330_001815"
     CDS             complement(22929..23924)
                     /gene="hlyD"
                     /locus_tag="OO330_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5882705.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23927..24598)
                     /gene="cecR"
                     /locus_tag="OO330_001816"
     CDS             complement(23927..24598)
                     /gene="cecR"
                     /locus_tag="OO330_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5882706.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24827..26014
                     /gene="rhlE"
                     /locus_tag="OO330_001817"
                     /pseudo
     CDS             24827..26014
                     /gene="rhlE"
                     /locus_tag="OO330_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26221..26703)
                     /gene="ybiA"
                     /locus_tag="OO330_001818"
     CDS             complement(26221..26703)
                     /gene="ybiA"
                     /locus_tag="OO330_001818"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5882707.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26823..28973
                     /gene="dinG"
                     /locus_tag="OO330_001819"
     CDS             26823..28973
                     /gene="dinG"
                     /locus_tag="OO330_001819"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5882708.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29001..29963
                     /gene="ybiB"
                     /locus_tag="OO330_001820"
     CDS             29001..29963
                     /gene="ybiB"
                     /locus_tag="OO330_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5882709.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30104..31189
                     /gene="hcxB"
                     /locus_tag="OO330_001821"
     CDS             30104..31189
                     /gene="hcxB"
                     /locus_tag="OO330_001821"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5882710.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31418..31678)
                     /locus_tag="OO330_001822"
     CDS             complement(31418..31678)
                     /locus_tag="OO330_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5882711.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31942..32208)
                     /gene="ybiI"
                     /locus_tag="OO330_001823"
     CDS             complement(31942..32208)
                     /gene="ybiI"
                     /locus_tag="OO330_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5882712.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32282..32959)
                     /gene="ybiX"
                     /locus_tag="OO330_001824"
     CDS             complement(32282..32959)
                     /gene="ybiX"
                     /locus_tag="OO330_001824"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5882713.1"
                     /translation="MMYHIPDVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33001..34985)
                     /locus_tag="OO330_001825"
                     /pseudo
     CDS             complement(33001..34985)
                     /locus_tag="OO330_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8289 a         8972 c         9452 g         8272 t
ORIGIN      
        1 atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata ttaacatctg
       61 cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg ttagggatat
      121 atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt tccccaacgt
      181 ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta tcaaaagcac
      241 ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg ttgccaacag
      301 aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg agaagggctg
      361 agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa
      421 catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg ttatcgctgg
      481 ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca ttgctagaga
      541 tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg agtaaactta
      601 aattgagggc tggctcttga aaagccagac atattttcag taatcgaact gcctgtgttc
      661 ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga taatccgtga
      721 tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag gagcggaaaa
      781 aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata tattaatata
      841 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
      901 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      961 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
     1021 caccacagca tcatttctct gcgcttatcg agatactgag catggttgta aattcagcta
     1081 acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg cgctaatgct
     1141 ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga tatgttgtgt
     1201 tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat tgatattaat
     1261 gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg ggaaggtaaa
     1321 aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc gccatctggc
     1381 agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg aaatgaacgt
     1441 taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct acatccagcg
     1501 cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg ccatcgtatc
     1561 cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct accagaccag
     1621 agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact acccagtgcc
     1681 accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc
     1741 ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg taacccatgc
     1801 cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta ctgatgagtt
     1861 tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac gctgacttcc
     1921 cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc ggtcagacgc
     1981 tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt gccaaaaggc
     2041 cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt caccggacga
     2101 gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat ttcggcatca
     2161 cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccatctg ctgctgccat
     2221 tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag cggattgccc
     2281 ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt ttctgcaacc
     2341 tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc tttaccgagg
     2401 cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc ggtacgacca
     2461 aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca cattttgcgg
     2521 attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg gacaatcggc
     2581 tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc aaaaccaacc
     2641 atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc aaacaagttt
     2701 tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca caccgacatc
     2761 gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg gcgcgcttcg
     2821 cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc tacggaaccg
     2881 gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag tttgcggcat
     2941 agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat ggcatcaatt
     3001 tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc cgcccaccag
     3061 gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca accttcggcg
     3121 ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg ccagatatgg
     3181 cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa attgaaaaga
     3241 tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga gaagccccat
     3301 ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa aaccgttgct
     3361 ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc gtcaggtgca
     3421 ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca aatactgccc
     3481 gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag ttgaacaggt
     3541 gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta tgggcgcggc
     3601 atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc agggggtaaa
     3661 agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc aggcgcagcg
     3721 cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc cggagtttta
     3781 cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg aaaaagtgcg
     3841 cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa cggtaaaaga
     3901 tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa gcgtaccaat
     3961 caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg ttgatgcctt
     4021 tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt acgtgcgcct
     4081 ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta tggcaggcgc
     4141 aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag aagataaaga
     4201 cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc tggcagggga
     4261 taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg acgaatatta
     4321 caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga tgcgcggcgt
     4381 gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg ctggctggtg
     4441 gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt aagccatcat
     4501 ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg tagcggcggc
     4561 tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga actggccgag
     4621 tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa tcaggcagtc
     4681 attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag ccatgcctca
     4741 ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca taacgatgtc
     4801 gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt ggtgacagaa
     4861 ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca ggtaacgcaa
     4921 cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat cggggagcag
     4981 gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt gacttttggc
     5041 aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc ggattatctg
     5101 ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca ggcgcaggca
     5161 ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg cgaaaaactg
     5221 gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac gctggctgat
     5281 tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt acaactggca
     5341 gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac cgtacccgct
     5401 ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga tatcgaccgt
     5461 ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg catttggtcg
     5521 ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg acgccttact
     5581 ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg gacctggctg
     5641 gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc tctcgccgcc
     5701 aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag gagatatcga
     5761 atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg cggtgcagtg
     5821 gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc ccggaggcgt
     5881 ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg cgcggcaggc
     5941 ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg aagagtcgct
     6001 gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg atgatgcgct
     6061 cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag ggcgcgaccc
     6121 gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc aacaacaggg
     6181 gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt aaacgttatt
     6241 ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca cttttacaag
     6301 ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct ggcagcgaaa
     6361 agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac agcagcctgc
     6421 agctggatta cgcaacggta aatccttaca ccttcgcaga acccacctcg ccgcacatca
     6481 tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga ttacgcgcgc
     6541 ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt acgccgcttt
     6601 ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg atactggtag
     6661 ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca atacaacacg
     6721 ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga aaacgtcacg
     6781 ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga gagatcccct
     6841 ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gtcagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tgtccgccac
     7861 caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg aatatctcga
     8881 agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg agcgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca acttactgcg
     9001 tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agttgcgtga
     9481 gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa cggaaaacag
     9541 tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa tttgcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcaacagg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgtt
    10381 cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc atttgaggcc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt gattacgggt
    12241 ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt gttcgaccgt
    12301 gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt attcaggcgg
    12721 caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggcgga
    13321 gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc ataacctggg
    13381 cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatct
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg cagatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc ttcgcttggc
    15541 tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa tacaggacgc
    15601 gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca gccagtagtg
    15661 cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg acgccagcaa
    15721 tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga ccaagtaaca
    15781 gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc tgagcgaggg
    15841 cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg cggtgcttcg
    15901 cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg ccgagaatac
    15961 gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct gcggtaaaga
    16021 taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc gaggaaatcc
    16081 gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc ataccaatgc
    16141 cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac accagcatca
    16201 cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca tagcagccgt
    16261 aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg cgattgtagt
    16321 cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc accagcaaca
    16381 caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc cagcgcgggt
    16441 gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat cctgcgtttc
    16501 cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg ccggaagcca
    16561 gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt tggtcaggtt
    16621 ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg cggcaataat
    16681 gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga tgacatttgc
    16741 ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg tcgcccagtg
    16801 atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa aaacctgaac
    16861 gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa tcggcatatc
    16921 cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt tacgcaaggc
    16981 gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt cccgctgcgc
    17041 ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt cgttatcgcg
    17101 ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt tgtgatgacg
    17161 tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa actggagaat
    17221 atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct ctggaccgta
    17281 gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac gcgcgtcgat
    17341 caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa aaaggcgagg
    17401 gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga caaactcatc
    17461 gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc cgcgttgcgc
    17521 tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc agataaacgt
    17581 ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg gataatattg
    17641 ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt tcataaatga
    17701 atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag cggcaacgcg
    17761 gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag tagaggaaat
    17821 tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc atccagtccg
    17881 gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc acccgccacc
    17941 aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc aagctgcgcc
    18001 tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc ttttccggtc
    18061 atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc gaccgaaaca
    18121 tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc gtgatgccct
    18181 tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc ggcgagaaac
    18241 tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc gtgcgcgccc
    18301 atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc gcgaagttcc
    18361 ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat ggtgagcacc
    18421 ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc tcttatctca
    18481 ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt ccgtaaagtt
    18541 cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag tggcaacaac
    18601 gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta ctggtagtgg
    18661 cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg ggattatgcg
    18721 cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc gagcaacgct
    18781 ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt ggcgcagcgc
    18841 tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg gcaattgcag
    18901 ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat aagaagttac
    18961 atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt tatcaccagt
    19021 agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata aatctgcttg
    19081 gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag ccacactggc
    19141 atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat aaaaacaaac
    19201 acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag accgaatccc
    19261 accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga tccggcgaag
    19321 gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa ggtggcgaca
    19381 attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg cgaaaccagt
    19441 agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt gacgatcatt
    19501 acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt gtagtcgaga
    19561 ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg ttttccttcc
    19621 agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc aatttgcgca
    19681 ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa ggtatccagt
    19741 ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt gtcgatggtc
    19801 gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct ggcgcgggca
    19861 aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat ggcgatggtg
    19921 gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat tagcacgggt
    19981 aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc tttgcggatt
    20041 aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt tggttttcag
    20101 ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt ttaccaccag
    20161 caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga aataacgagc
    20221 gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct gaaaaataaa
    20281 gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag cggcattgaa
    20341 ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga gtaaaaacag
    20401 gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga gaataaacac
    20461 tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag ggatcagctt
    20521 acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca tggtgccgcg
    20581 ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg tcatgataat
    20641 ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa accagtagcg
    20701 ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc gctgcatttg
    20761 ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat tcggctcact
    20821 gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct gttcggcgaa
    20881 atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg cgatcagttc
    20941 ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg tgtgggtgaa
    21001 atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct tgctggagtc
    21061 gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta ccgcaatcag
    21121 ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg cccgcacgcg
    21181 atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg tggatcaact
    21241 gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac tgtgctttca
    21301 aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg atgcggtcgc
    21361 aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct ttctctacca
    21421 tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg gaagtcggtt
    21481 cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc agccgctgtt
    21541 taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg ccaaatgcct
    21601 cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac acaccagaga
    21661 aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt tgcgccatat
    21721 agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc agcaccagcg
    21781 cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc gatttacccg
    21841 cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag ttgacgtgat
    21901 cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc accgtctcgc
    21961 cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag gttccggcac
    22021 cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg atgttgattt
    22081 ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg aggatcagac
    22141 gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag gcgcgttgca
    22201 acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg gccatggttt
    22261 gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc agtaacacgt
    22321 cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac atcccttcgc
    22381 ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca acgccgacgc
    22441 cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc aggccgagtt
    22501 tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc ccaagagacg
    22561 taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg ctgcgcaaat
    22621 ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca aatttctgcg
    22681 gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca aagccaatca
    22741 ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc agtgtggttt
    22801 tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg gtacaatcga
    22861 gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg ttcagcgtga
    22921 taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg cattccctgg
    22981 cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag gtcggtacgc
    23041 agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac gaaaccaatc
    23101 tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt gcgccccggc
    23161 tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg acgcgttagt
    23221 gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac cgcgcgcgtt
    23281 aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag ctccgcctgc
    23341 gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc ctgttcacgg
    23401 ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc ctgtgcctgg
    23461 tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct tttccacagt
    23521 ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc ctgtttcacc
    23581 gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag atcatactgc
    23641 gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata cggcttgtga
    23701 tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac cgccagcgat
    23761 tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac gttgccatac
    23821 agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc aaccacggcg
    23881 gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac aaactccttt
    23941 gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg atcagttcgg
    24001 tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct ttgccaagac
    24061 ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg gtgtcattag
    24121 cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc ggactaatca
    24181 cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg gagataaact
    24241 tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg caggcgcgaa
    24301 ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag cgttcggctt
    24361 cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg gcgcaggcga
    24421 ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc tgcccggcct
    24481 gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc gccagtgcgg
    24541 cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga ttattcatcg
    24601 cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg gagttgtcca
    24661 gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct gcgtcacaaa
    24721 aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct tttcagtatc
    24781 tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt ctttcgattc
    24841 tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc gtgaacccac
    24901 ccctattcag cagcaggcga tccctgcggt gctggaaggc cgcgacctga tggctagcgc
    24961 ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac acctgatcac
    25021 tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta ccccgacccg
    25081 tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc tgaacattcg
    25141 ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt tgcgcggcgg
    25201 cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc aaaatgcagt
    25261 gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc tcgacatggg
    25321 ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc agaacctgct
    25381 gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt tgcacaaccc
    25441 gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc agcacgttca
    25501 ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta aagggaactg
    25561 gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg ctgagcagct
    25621 caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag gtgcgcgtac
    25681 ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga ccgatatcgc
    25741 tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac tgccaaacgt
    25801 accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta ccggtgaagc
    25861 gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa aactgctgga
    25921 aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa tcaaagccga
    25981 accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca aacgcaaaac
    26041 tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg aatttgcatg
    26101 cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca agcacttgtc
    26161 agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc caccgagacg
    26221 ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca gtcgattctt
    26281 gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct caaccagttt
    26341 tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga atttagcgcg
    26401 aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa gtggcttgga
    26461 acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga tctcttcgcg
    26521 gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg gccaggtatt
    26581 cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt catcgctggt
    26641 gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg ctcgaacggg
    26701 cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca gaaaaaccat
    26761 tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt tcaggttttc
    26821 tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag gcgcttcagg
    26881 aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg gacgtcgcca
    26941 aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc ggcgttggga
    27001 aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg
    27061 tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat ttaccgctgc
    27121 tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg cgctacgttt
    27181 gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat ctgctggcgt
    27241 ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt gcgaagctga
    27301 agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat atcgctatag
    27361 atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac cgtaactgtt
    27421 actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa gcggaagtgg
    27481 tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta ctgcctgacc
    27541 cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg gcccgggatg
    27601 cgctggagat gagcgctgaa atcaccgcgc cctggtatcg actacagctg gacttgttca
    27661 cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca ccgctggcga
    27721 tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc tcgttaaaca
    27781 acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt gcgatgggcg
    27841 aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc gagatgctgc
    27901 gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc cacgatattg
    27961 tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt gaggtgcaaa
    28021 gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg accaaatggg
    28081 cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg ggaatacgtg
    28141 tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt gtcacctccg
    28201 caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt ctgaaagaga
    28261 aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa
    28321 ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa cacattgccg
    28381 aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt atgttggtac
    28441 tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat ttacgcctga
    28501 tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac cgcaaacgcg
    28561 ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa gggcttgatt
    28621 tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg cccatcgaca
    28681 gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat ccgtttgagg
    28741 tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga ctgattcgaa
    28801 gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca aaaaattatg
    28861 gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa gtccctgaag
    28921 gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg
    28981 agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc gggcgcggga
    29041 aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct catatgctca
    29101 atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt atcaaagggg
    29161 aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc atcaagctga
    29221 cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc gcgcgtaaac
    29281 aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt cctgtggtgg
    29341 ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc gaactgatgg
    29401 gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg
    29461 tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg atgcgctggc
    29521 ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca tttgccgaag
    29581 gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc gtcgcgaagt
    29641 tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc gaagtgtatg
    29701 ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg cgggtgctgt
    29761 atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat ccggaaacca
    29821 cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa
    29881 tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc gatggcctgg
    29941 cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt catccggctt
    30001 ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc ccggcgtgca
    30061 gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa gtggtcatcg
    30121 ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga tgggtagcga
    30181 ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg cagggcatga
    30241 ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca
    30301 aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga
    30361 tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg agaaagcgca
    30421 tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc gtatcggtta
    30481 ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta gcgtggtcgg
    30541 tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca atcccttctg
    30601 tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca ccagcgccat
    30661 tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc caggttgcct
    30721 gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt cgccgttggg
    30781 gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt gtgaaattct
    30841 tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga
    30901 tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg gcgcgccgga
    30961 ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc atgatgacga
    31021 taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca
    31081 ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc ggcagattgg
    31141 tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa aaaaagcccg
    31201 tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag agcatatgcg
    31261 tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg cctgatccgt
    31321 ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt
    31381 gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt accgcagtac
    31441 cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca ccggctgccg
    31501 ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca tcggcagaaa
    31561 ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc gccgcgaaca
    31621 cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata gttttcataa
    31681 ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc
    31741 acgtttttta cgagagataa aatcgaagag aattgaccgc cttgttcaaa taaattgatt
    31801 gatatctaac atactgataa accatttatt tgttgtactt atggacttgt tttatgccgc
    31861 gtggcagtca cagcagcgtt aaaggcacgg aatgccacgc aaaatacaaa ttatactcgc
    31921 tatgcatctc gccagttgta gttaacgtaa ctgtctgtct ttcgaacctc tgcgattata
    31981 tcctgtatac gctggttttt gtaaatcttt ctcctgctga caatgaatac ataagcgcac
    32041 gccaggaatg gcttcccgac gggcttgcgg gatgggggca ccgcactctt cacattcata
    32101 caggctttcg ccacgcggaa tttcaccccg agcgcgggca atcgcatctt caattgtact
    32161 gttgatctgt tcgttgacgg cgtcatcgtt agcccaagcg gatgccatat cgtcctcccc
    32221 atatcaatgc ttgtacagtt aagtgtagct aatataggga cgaacctggg cagttcaagc
    32281 gtcagatctc cgaccattcc cgcagcagat tatgataaag attgagcagc gacaggatct
    32341 cttcattttc accatagcgg cttttcagcg actgaatatt tttgtccagt tcaaacagca
    32401 tggcgcgctt tttatcatcg cggatcatcg actggatcca cataaatgat gccactcgta
    32461 cgccgcgggt tacgggtgtc acgcaatgca ggctgctgga gggatacaac accagatcac
    32521 ctgccgggag ttttacccga tgttgtccaa aggtgtcatt aacgaccagt tcgccgccgt
    32581 cgtagctttg tggatcgctt aaaaacagcg tggcagaaag gtcagtgcgc atccagccgt
    32641 tttgtggatg gctgcgtact gcgccatcca catggaaacc ataggtttca ttgttctgat
    32701 agcgattaaa cagcggcgtg gaaagggtac gcggcaaggc agcggcaaag aacaatgcat
    32761 gttggttaac cgcgttcagc acctcatttt gcagtgcggc gtataacgcg ctgcgggtgt
    32821 ctacttgctg attgttctta acttgtgcac cttgtgctcc ggtggtgacg cgtccatcca
    32881 cccattcggc ttgttccagt tgttcgcgaa aatgggcgac gtcctgtggc gataacacgt
    32941 cgggaatgtg gtacatcata caagttctcc aaaaagtggg gcctgcgccc cacatctgaa
    33001 tcagaaatgc atattggctg tgagcaagaa ggttcttggc tcgcccgggt gataacggta
    33061 gccgctcttg ttgattgagg cgacgtaatc ggtatcaaac aggttgtaaa cgtttagctg
    33121 gaagtcgaga ttgcgattaa cccgataccc cagtttggca tcggcgaccc agtaaccttc
    33181 tgtaaatgct ggcgttccca ccgcgccgtc tgaaccttta tgtatactgc cgatatagcg
    33241 tgcgcccgcg ccaacagaga tatcatcggt tgcctgatat tggctccata aggtgaaggc
    33301 gtgctccggg gtatacggca gcgatgagga accatcctgg gcaacatctt tgccgttttt
    33361 gatggttgct ttttgctggg tatagccgcc aatcacctgc cacgcgggag tgatattccc
    33421 tgccacggat atctcatagc cttcgacgcg tttcttaccg tattgcgagt aagtcccgtc
    33481 atcattttgc tcaacttcat tttcgatatc agtacggaac agcgcggcgg tgagcaacag
    33541 gcgtttatcc agaacctgcc atttggtgcc aatctcgctg gtgttggctt tttgcggttt
    33601 aaaatcggtg tggttggcac tgttaccact gccagactgc gcaagggcga agttgttgcc
    33661 gcccggaggc tgctgggaaa cggcatagtt aatatagaca ttgccgtttt ccgtcaggtg
    33721 gtacagcgcc ccggctttcc agttcaccag attgcccgac ttggcggtgt cgacggtggt
    33781 gactggagaa ccttttgcca caccagctgg gcaggtgatg gcaccgcgtc cgctgccgcc
    33841 gcaggcggtg gcactgtcat attcagtatg ataattatcc agacggatcc cgccgttcag
    33901 ctcaaaatca cgggtgattt gcagcgtatc gaaggcgtaa attgcgaagg tatccgtctg
    33961 accattggcg tttgcgccgt tgcgcgtcag gccgccggga tgaattctgc tgtcaggatg
    34021 ataaatattt accgccggta acgtcaccgg attaacgcca tagttagtct gcgtttcacg
    34081 ggtaaattcc acgccggtac tgacatcatg accgatagaa ccggtataga acgtcgaggt
    34141 caggttggtc tggttggtga gaattttatt actcacatct ttggtattcg ccgtgcgtga
    34201 ctaggtccag ctattcacat cgctggtggg ctgagtaata ttcgacgccc cgcccataat
    34261 cgccgtcatc aggtaatcct gctttacgcg cgaccaacgg gtagtattgc gaatggtggt
    34321 gttatcgttg atgtcgtgct caaaacgcat ggtggcggtg tcggtggtcg aatcgtcgta
    34381 atcggaatcc gtgccgtaaa agttatgagt atcaactttt ccggaatgat tcagggccgc
    34441 cgctcccgca gatggggcag aatagcccgg caaaccgatg gtcggaatgc cgccgtctgg
    34501 cgtgttgtgc tgagtgacat gcagataatt aagatacaaa cgatttgctg taccaaggcc
    34561 aaaagcgaca gaaggggcga cgccgtaacg ctcatttttg actttgtcgc gtccggcatc
    34621 gtgggttttt tcgcccatca cattcaggcg caccgcggta gtatcaccaa tgacctgatt
    34681 gacgtccagc gtgccgcggc ggaaccaggc gctgccaata ctggcggagg cgtcaatgcc
    34741 ggaatcattg cgcggctgct tgctgatcat attgatcgag cctgtcggtg cgctgcgccc
    34801 gtagtcggtg ccggacggcc ctttaatcac ttcgacctgc tcggtattga aggtgtcgcg
    34861 cgagacgctg ccgatatcgc gaataccatc aatataaata ctgttagagg tatcggcacc
    34921 acgcatataa atggcgtcgc cagtggtgga gttaccgttc tcacccgcaa aaaacgcacc
    34981 cacgc
//
LOCUS       DAJTRB010000035        34914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTRB010000035 DAJTRB010000000
VERSION     DAJTRB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.035"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            868..3153
                     /gene="nrdA"
                     /locus_tag="OO330_001826"
     CDS             868..3153
                     /gene="nrdA"
                     /locus_tag="OO330_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5882714.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3387..4517
                     /gene="nrdB"
                     /locus_tag="OO330_001827"
     CDS             3387..4517
                     /gene="nrdB"
                     /locus_tag="OO330_001827"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5882715.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4517..4771
                     /gene="yfaE"
                     /locus_tag="OO330_001828"
     CDS             4517..4771
                     /gene="yfaE"
                     /locus_tag="OO330_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5882716.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4825..5475)
                     /gene="inaA"
                     /locus_tag="OO330_001829"
     CDS             complement(4825..5475)
                     /gene="inaA"
                     /locus_tag="OO330_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5882717.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5654..5896
                     /locus_tag="OO330_001830"
                     /pseudo
     CDS             5654..5896
                     /locus_tag="OO330_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5938..7014)
                     /gene="glpQ"
                     /locus_tag="OO330_001831"
     CDS             complement(5938..7014)
                     /gene="glpQ"
                     /locus_tag="OO330_001831"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5882718.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7019..8377)
                     /gene="glpT"
                     /locus_tag="OO330_001832"
     CDS             complement(7019..8377)
                     /gene="glpT"
                     /locus_tag="OO330_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5882719.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8650..10278
                     /gene="glpA"
                     /locus_tag="OO330_001833"
     CDS             8650..10278
                     /gene="glpA"
                     /locus_tag="OO330_001833"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5882720.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10268..11527
                     /gene="glpB"
                     /locus_tag="OO330_001834"
     CDS             10268..11527
                     /gene="glpB"
                     /locus_tag="OO330_001834"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5882721.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLIDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11524..12714
                     /gene="glpC"
                     /locus_tag="OO330_001835"
     CDS             11524..12714
                     /gene="glpC"
                     /locus_tag="OO330_001835"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5882722.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12907..13857
                     /locus_tag="OO330_001836"
     CDS             12907..13857
                     /locus_tag="OO330_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5882723.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13898..14368)
                     /locus_tag="OO330_001837"
     CDS             complement(13898..14368)
                     /locus_tag="OO330_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5882724.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14423..15625)
                     /locus_tag="OO330_001838"
     CDS             complement(14423..15625)
                     /locus_tag="OO330_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5882725.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15712..16254)
                     /locus_tag="OO330_001839"
     CDS             complement(15712..16254)
                     /locus_tag="OO330_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882726.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16533..16958
                     /gene="nudI"
                     /locus_tag="OO330_001840"
     CDS             16533..16958
                     /gene="nudI"
                     /locus_tag="OO330_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5882727.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16997..17599)
                     /locus_tag="OO330_001841"
     CDS             complement(16997..17599)
                     /locus_tag="OO330_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5882728.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17907..19046
                     /gene="arnB"
                     /locus_tag="OO330_001842"
     CDS             17907..19046
                     /gene="arnB"
                     /locus_tag="OO330_001842"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5882729.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19050..20018
                     /gene="arnC"
                     /locus_tag="OO330_001843"
     CDS             19050..20018
                     /gene="arnC"
                     /locus_tag="OO330_001843"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5882730.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20018..22000
                     /gene="arnA"
                     /locus_tag="OO330_001844"
     CDS             20018..22000
                     /gene="arnA"
                     /locus_tag="OO330_001844"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5882731.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21997..22887
                     /locus_tag="OO330_001845"
     CDS             21997..22887
                     /locus_tag="OO330_001845"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5882732.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22887..24539
                     /gene="arnT"
                     /locus_tag="OO330_001846"
     CDS             22887..24539
                     /gene="arnT"
                     /locus_tag="OO330_001846"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5882733.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24536..24871
                     /gene="arnE"
                     /locus_tag="OO330_001847"
     CDS             24536..24871
                     /gene="arnE"
                     /locus_tag="OO330_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5882734.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24871..25257
                     /gene="arnF"
                     /locus_tag="OO330_001848"
     CDS             24871..25257
                     /gene="arnF"
                     /locus_tag="OO330_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5882735.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25251..25523)
                     /gene="pmrD"
                     /locus_tag="OO330_001849"
     CDS             complement(25251..25523)
                     /gene="pmrD"
                     /locus_tag="OO330_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5882736.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25628..26983)
                     /gene="menE"
                     /locus_tag="OO330_001850"
     CDS             complement(25628..26983)
                     /gene="menE"
                     /locus_tag="OO330_001850"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5882737.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26980..27942)
                     /gene="menC"
                     /locus_tag="OO330_001851"
     CDS             complement(26980..27942)
                     /gene="menC"
                     /locus_tag="OO330_001851"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5882738.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27942..28799)
                     /gene="menB"
                     /locus_tag="OO330_001852"
     CDS             complement(27942..28799)
                     /gene="menB"
                     /locus_tag="OO330_001852"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5882739.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28814..29572)
                     /gene="menH"
                     /locus_tag="OO330_001853"
     CDS             complement(28814..29572)
                     /gene="menH"
                     /locus_tag="OO330_001853"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5882740.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29569..31239)
                     /gene="menD"
                     /locus_tag="OO330_001854"
     CDS             complement(29569..31239)
                     /gene="menD"
                     /locus_tag="OO330_001854"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5882741.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31328..32623)
                     /gene="menF"
                     /locus_tag="OO330_001855"
     CDS             complement(31328..32623)
                     /gene="menF"
                     /locus_tag="OO330_001855"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5882742.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32702..33007)
                     /gene="elaB"
                     /locus_tag="OO330_001856"
     CDS             complement(32702..33007)
                     /gene="elaB"
                     /locus_tag="OO330_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5882743.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33062..33523)
                     /locus_tag="OO330_001857"
     CDS             complement(33062..33523)
                     /locus_tag="OO330_001857"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5882744.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33588..34505
                     /gene="rbn"
                     /locus_tag="OO330_001858"
     CDS             33588..34505
                     /gene="rbn"
                     /locus_tag="OO330_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5882745.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34696..34884
                     /locus_tag="OO330_001859"
                     /pseudo
     CDS             34696..34884
                     /locus_tag="OO330_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8538 a         9344 c         8650 g         8382 t
ORIGIN      
        1 tccagtcctg cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat
       61 catatccctg gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa
      121 tcattgacgg gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct
      181 gaccatcatt ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc
      241 ttttgtaaat tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc
      301 agtgcgtcat aattcaagtt aataacctta agggatacca gttatattta aactaaatta
      361 aagtaatgaa taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac
      421 aataaccgaa tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat
      481 tgaccacaac tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc
      541 attagcaaaa agattaataa accatctata tcaatttatc caacctattc tgccgttcaa
      601 gaaatcgccg aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac
      661 cttgtctgac ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta
      721 tgcacttgca agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta
      781 tatatagtgt tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa
      841 agaattctaa aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac
      901 ggtagcacag agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa
      961 ggactgcata acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac
     1021 ggtatcaaga cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc
     1081 cgtgatgcgc cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa
     1141 aaagcctacg gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag
     1201 atgggcaaat acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg
     1261 gacacgttta tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg
     1321 gaaggcaaat atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc
     1381 ctttatattc ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa
     1441 tacgtgaagc gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc
     1501 atgtccggcg tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt
     1561 gacagcctgg attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt
     1621 gccgggatcg gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc
     1681 gaagcgttcc ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc
     1741 tgctctcagg gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg
     1801 gaagtggaaa gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt
     1861 catatggact acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa
     1921 gatatcaccc tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat
     1981 caggaagagt ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag
     2041 cgtgtgaaag ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt
     2101 atctatattc agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg
     2161 ccagttcgcc agtctaacct gtgcctggaa attgccctgc cgaccaaacc gctgaacgac
     2221 gtcaacgacg agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca
     2281 attaatagcc tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg
     2341 ctactggatt atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt
     2401 acgctgggta ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac
     2461 tccgacggca gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg
     2521 ctgaaagcgt ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc
     2581 acttacgcga aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct
     2641 aatgagccgc tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg
     2701 cgtaactcca cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc
     2761 actaacggta ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt
     2821 ttgcgccagg tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa
     2881 atgccgggta acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag
     2941 tcgatctctg ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg
     3001 cagcagttgc tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat
     3061 cagaacaccc gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac
     3121 gatggctgcg aaagcggcgc atgtaagatc tgatattgag atgccggatg cgccgtaaac
     3181 gccttatccg gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag
     3241 gctccgggtg ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc
     3301 ctgataagac gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg
     3361 cattaaactc ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg
     3421 atcagctcaa agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc
     3481 aaaaatatga catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg
     3541 aagaagttga cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac
     3601 acatctttat cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc
     3661 cgaacgtggc gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct
     3721 gggcgttctc agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta
     3781 acgatccgtc tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg
     3841 aagggatctc cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg
     3901 gcgaaggcac ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga
     3961 aaaaactgta tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca
     4021 gctttgcttg ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta
     4081 ttcgcctgat tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc
     4141 tgctgcgcag cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg
     4201 agtgctatga cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt
     4261 tccgtgacgg ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca
     4321 tcaccaatat ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc
     4381 cgatcccgtg gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag
     4441 tggaagtcag ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga
     4501 gtaacttcca gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg
     4561 ccaggatgaa cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta
     4621 ccagtgccgc gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga
     4681 ctggattgcc gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg
     4741 ggcaaaaggc gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta
     4801 cgcgtattat ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca
     4861 gtccgcttta gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc
     4921 tttatcgcga cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt
     4981 accttctgtt ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct
     5041 atgcattttc ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga
     5101 ataaggcgat actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc
     5161 ttcagtcacc agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat
     5221 cttcggcacg atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat
     5281 cgttggtcgg ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac
     5341 atacagcttt ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata
     5401 gttaggctct tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt
     5461 tgctgaaact gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg
     5521 ttacctgagg aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag
     5581 gtgtgacacc gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta
     5641 atagtgcgcc cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat
     5701 tcgacaggga ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga
     5761 attgtgttcc gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga
     5821 aaagccggta gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc
     5881 gattggaaaa atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta
     5941 ctctttatta agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc
     6001 cgctttgtta tacagagcat catataactg attcacatca ggagtgtatt caggcagttt
     6061 atctgaccgc acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat
     6121 gccagtgagt ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg
     6181 accaatacca tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc
     6241 gtagttgtaa ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt
     6301 ataggcaatc agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt
     6361 aatacgcttc agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt
     6421 gtaaccatat ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg
     6481 atggaaccac ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt
     6541 taacccctga acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc
     6601 cattgggaaa cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc
     6661 ggtaaatttc agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc
     6721 tttgcgcgcc cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc
     6781 atgcagaaca accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc
     6841 tccctgcgca tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc
     6901 accgcgatgg gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat
     6961 gactatagtg ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt
     7021 agcctccgtt gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa
     7081 caatcaacaa gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc
     7141 agccgaagaa gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt
     7201 aaccaaacag cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca
     7261 gcgcatgcag accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac
     7321 aaatcatatc gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg
     7381 tcaccagtgt cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg
     7441 acatccagcc gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc
     7501 aggaggattt atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga
     7561 ggatgccgta acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt
     7621 tgttcggcag cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt
     7681 cgttatagtc gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt
     7741 gcggggtatc gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag
     7801 gcatatagag cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg
     7861 gcggaatacc accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt
     7921 ctttctgcga ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga
     7981 accaaccgca gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca
     8041 caaagcccat aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc
     8101 gcggattcga gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg
     8161 agatccccga aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag
     8221 gcatagcaag cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc
     8281 ccaggaaaat ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc
     8341 gcgctttgtg tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt
     8401 cttatttatg attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg
     8461 atgttaagaa aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt
     8521 tacaaaatgt tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat
     8581 gcgcgaaatc aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga
     8641 gggataacaa tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc
     8701 gcaacgggag ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt
     8761 gagcgccacg acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt
     8821 gcgcgctatg cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc
     8881 ctgaaacgca ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg
     8941 gaagatgacc tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc
     9001 gcagaagcta tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg
     9061 attggcgcgg tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac
     9121 atgctggatg ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg
     9181 attcgtgaag gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact
     9241 caggcccttc atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc
     9301 gaatatgccg atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat
     9361 caccgcatta accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg
     9421 gtgcctggcg ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag
     9481 attgacgata atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa
     9541 ctggcccccg tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg
     9601 gttgccagcg atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac
     9661 catgctgaac gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc
     9721 tatcggctga tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc
     9781 ccctgtacga ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg
     9841 cgtaaagtca tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat
     9901 cgcacgcctg cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa
     9961 gcggtaactg cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg
    10021 gatttacgcc gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc
    10081 cgcgctgccg gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa
    10141 ctttccacct tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca
    10201 ctgcgcgaaa gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag
    10261 cagaaagatg cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg
    10321 cctgcaactg caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt
    10381 gcatttctca tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc
    10441 agacattcac agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct
    10501 tctcgggcca caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag
    10561 cggtgcgcaa ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac
    10621 tttgcgtgca acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa
    10681 gatatgtgta gtggggataa gcgggctgat agatttccag gcgcatcttg cggcggcttc
    10741 gttgcgcgaa ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt
    10801 cctgcgcaat aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga
    10861 agaaaactgg ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat
    10921 cctgatgccc gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa
    10981 actaccttgt tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct
    11041 gcaaaaccag ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga
    11101 agtgaaaaaa gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc
    11161 cgatattccg ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg
    11221 actggtagcg gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac
    11281 cgccacgcgg ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt
    11341 cggtgtaacc actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct
    11401 gtttgccatc ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg
    11461 tgtttgtgcc gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca
    11521 acaatgaacg acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc
    11581 ccggtgagcc gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag
    11641 cgtctgcgtt tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc
    11701 aaacgttgtg aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg
    11761 cgggcgaaat atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac
    11821 ctgatgggta gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa
    11881 ccggtgcggc agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa
    11941 tactccttcg gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat
    12001 aaagaccagg tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt
    12061 aaagatttaa ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa
    12121 aaatgctgcg gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca
    12181 attacgaatg tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc
    12241 acctcctcaa cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac
    12301 aacaaaggct tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac
    12361 gaaggcaaaa cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc
    12421 catatggaaa aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg
    12481 cttgagttaa cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa
    12541 aaagagaact accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa
    12601 agcggcgcag atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc
    12661 acaagtcttc gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc
    12721 ttctgatgcc cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca
    12781 atgtatgcgt tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg
    12841 gcagatgtca aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg
    12901 acaaccatga ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata
    12961 ttcacacccg aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag
    13021 ctttgcaacc tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc
    13081 gcttactact cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac
    13141 tgcgtgattg aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat
    13201 gccactgccg ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg
    13261 ccattgctgt tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat
    13321 gagtttgacg atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat
    13381 atcaccatcg tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg
    13441 attcaaaagc atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt
    13501 ttggttagag gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa
    13561 tgcggcgata cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta
    13621 caaaaggaga gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc
    13681 tggcaagaag gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt
    13741 atgcatgaac aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac
    13801 caagtgctcg cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta
    13861 acacaggccc acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg
    13921 gcccattttt gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca
    13981 tcgtgtcaac gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat
    14041 caggagccac agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg
    14101 tttcaataat tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag
    14161 aaagatccgc tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca
    14221 ggttatccag tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt
    14281 gcgccatgta attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac
    14341 cacgctcacc gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt
    14401 aaaccggcac agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc
    14461 agccatgctc actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca
    14521 tcattgcgca cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac
    14581 gcagagcgaa agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac
    14641 ccgaaacagc cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg
    14701 cagtttgcgc cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac
    14761 ctgcgcgaga aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc
    14821 tgaactggcg gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga
    14881 aaatcacgct ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt
    14941 gctcgcttgc cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca
    15001 ttgttacgcc cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac
    15061 gaccaaaggt agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca
    15121 ggcgcggcag gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa
    15181 agaacatcag gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat
    15241 tgataaattc agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc
    15301 gtccacgttc gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc
    15361 cttcaccttt tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc
    15421 cgccgttaac gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt
    15481 catcaaggtt atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa
    15541 aatcggccag ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg
    15601 tggatagcat ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa
    15661 cgcaaaggga gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact
    15721 ggcgttaacc ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc
    15781 cgacagattc aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg
    15841 cgcgccagca ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta
    15901 gtaatactcg ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac
    15961 cgctgcggca taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac
    16021 ggtcgccatt aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc
    16081 gtcgtcgtcg ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa
    16141 accgacacca atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac
    16201 cgatgcagaa accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc
    16261 ctttcccaca agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag
    16321 aggattcaat gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat
    16381 aattaaacgc cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc
    16441 agttatcatt gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc
    16501 atggtttctt tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt
    16561 tgattcaaaa tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc
    16621 ccggtcaatg ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac
    16681 tacgccgcga aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt
    16741 ggaccttcag cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt
    16801 atatgattta cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag
    16861 agtttcagga ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg
    16921 ccacccgaaa aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg
    16981 ctgtcagatt gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt
    17041 ccacatgcat gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt
    17101 tagcaatata tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt
    17161 tgctttgtaa gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat
    17221 ctaccgtcag ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat
    17281 tactggaata aagatcgaaa tcagggatgt cagcgctaaa agcgttgccc agttcacggg
    17341 catcctgggt acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac
    17401 gatcgcaacg ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg
    17461 ccaatgtttt attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca
    17521 gtccggcaat tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg
    17581 agcggcaaaa agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg
    17641 tgaaattgag cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg
    17701 atgtcggcaa aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa
    17761 aaatttcaat atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta
    17821 aaatttcgct ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca
    17881 ggtattcaat ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa
    17941 tgggcgtgga ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg
    18001 gtccgaagaa tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca
    18061 tcgcggtcag ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca
    18121 agggcgatga agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct
    18181 tgctgggtgc aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg
    18241 aagcgatcga atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg
    18301 gtgcgccagc agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta
    18361 tcgaagatgc tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag
    18421 gtaccgctat tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga
    18481 ttgtaactga taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg
    18541 gtgtcgatgc ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac
    18601 cgggctataa gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca
    18661 aattagagca cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg
    18721 cagctctccc ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc
    18781 tgtttattat tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag
    18841 cgttaaaaga aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat
    18901 attatcgcga gcgttttccc acactgtcgt taccgaatac cgaatggaat agcgaacgca
    18961 tctgttcgtt gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag
    19021 cccttcagca actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct
    19081 cggtggttat tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca
    19141 ccacagcctg tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta
    19201 gcgataattc cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt
    19261 ctattttgat taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg
    19321 ttactggcga cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc
    19381 cccgcctggt ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc
    19441 gccaggacag ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca
    19501 ccactggcaa agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg
    19561 tcgatgcgat gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct
    19621 tcgcccgccg tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca
    19681 aatacagttt tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa
    19741 cgccgctacg tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg
    19801 cggtgctgct ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct
    19861 ttatgctatt tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac
    19921 tcggtgaata tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc
    19981 agcaagttat ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta
    20041 ccacgatatg ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc
    20101 catttttacc cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct
    20161 ggcggcggaa agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt
    20221 ggaacgcatt gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat
    20281 ttgcgacgag atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt
    20341 accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac
    20401 gggcgttaca ttgcaccgaa tggtaaaacg tgccgatgct ggggccattg tggcgcaact
    20461 gcgcgttgcc attgcgccag acgatatcgc tattacgctg catcataaat tgtgccatgc
    20521 cgcgcgccag ctactggaac agacattacc cgccattaaa cacggtaata ttctggaaat
    20581 cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga acgccggatg acagtttcct
    20641 tgaatggcat aaaccggcat ccgtactgca caacatggta cgtgccgttg ccgatccgtg
    20701 gccgggtgcc ttcagctatg ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca
    20761 tcctcatgcc agcaaagcac agccggggag cgtgatttct gttgcgccac tgctgattgc
    20821 ctgtggcgat ggcgcgctgg aaatcgtcac cggacaggcg ggcgacggca ttactatgca
    20881 gggctcgcaa ttagcgcaga tgctgggcct ggtgcaaggt tcacgcttga atagccagcc
    20941 tgcctgcacc gcccgacgcc gtacccgggt actcatcctc ggggtgaatg gctttattgg
    21001 caaccatctg acagaacgcc tgctgcgcga agatcattat gaagtttacg gtctggatat
    21061 tggcagcgat gcgataagcc gttttctgaa tcatccacat tttcactttg tcgaaggcga
    21121 tatcagtatt cattccgaat ggattgagta tcacgtcaaa aaatgtgatg tcgtcttgcc
    21181 gctggtggcg atagccacgc cgattgaata tacccgcaac ccgctgcgcg tatttgaact
    21241 cgattttgaa gagaatctgc gcattatccg ctactgcgtt aagtaccgta agcgaatcat
    21301 cttcccgtca acttcagaag tttatgggat gtgtagcgat aaatacttcg atgaggacca
    21361 ttctaattta atcgtcggcc cggtgaataa accacgctgg atttattcgg tgtcaaaaca
    21421 attacttgat cgggtgatct gggcctatgg cgaaaaagag ggtttacagt tcaccctctt
    21481 cctcccgttt aactggatgg gaccacgact ggataacctt aatgcagcgc gaattggcag
    21541 ctcccgcgct attacgcaac tcattctcaa tctggtagaa ggttcaccaa ttaagctaat
    21601 tgatggcgga aaacaaaaac gctgctttac tgatatccgg gacggtatcg aggcgttgta
    21661 ccgcattatc gaaaatgcgg gaaatcgctg cgacggcgaa attatcaaca ttggcaatcc
    21721 tgagaacgaa gcgagcattg aggaactggg cgagatgctg ctggcgagct tcgaaaaaca
    21781 tccgctgcgc catcatttcc caccgtttgc gggctttcgc gttgtcgaaa gtagctgcta
    21841 ctacggcaaa ggatatcagg acgtagagca tcgtaaaccg agcatccgca atgcccaccg
    21901 ctgcctggac tgggagccga aaattgatat gcaggaaacc atcgacgaaa cgctggattt
    21961 cttcctgcgc accgttgatc ttacggataa accatcatga ccaaagtagg cttacgcatt
    22021 gatgtcgata cctttcgtgg cacccgtgaa ggcgtgccgc gtctgctgga aatcttgagt
    22081 aagcataata ttcaggccag catttttttc agcgtcggcc cggacaatat gggccgccat
    22141 ctctggcgac tggtgaagcc acagtttttg tggaagatgc tgcgctcaaa cgcggcatcg
    22201 ctttatggct gggatatttt actggcaggt acggcctggc caggtaaaga gattggtcat
    22261 gccaatgccg atatcattcg tgaagcggct aaacatcacg aagtcggtct gcacgcctgg
    22321 gatcaccatg cctggcaagc ccgtagcggt aactgggatc ggcaaacaat gatcgacgat
    22381 attgcacgcg gtcttcgcac tctggaagag attatcggtc aaccggtaac ctgttctgcc
    22441 gctgcgggct ggcgtgccga ccagaaggtg atcgaagcaa aagaagcgtt ccatttgcgc
    22501 tacaacagcg attgtcgtgg ggccataccg ttccgtccac tgcttgaatc aggaaaccct
    22561 ggcactgcgc aaattccggt gaccttaccc acctgggatg aagtgattgg tcgggatgtg
    22621 aaagcagaag attttaacgg ttggttactc aaccgcatcc tgcgagataa aggcacgccg
    22681 gtttatacca ttcatgcaga agttgaaggc tgcgcttatc agcataattt tgtggatctc
    22741 ctcaaacgcg cagctcagga aggcgtgaca ttttgccctt taagcgaact gttatcagag
    22801 acgttgccgc tcggacaagt tgttcgcgga aatattgccg gacgtgaagg ctggctgggt
    22861 tgccaacaaa ttgcgggtag tcgctgatga aatcggtacg ttaccttatc ggcatcttcg
    22921 cgtttattgc ctgctattac ctgttaccga tcagcacgcg tctgctctgg caacccgatg
    22981 aaacgcgtta tgcggaaatc agtcgagaaa tgctggcatc cggcgactgg attgtgcccc
    23041 atctgttagg gctacgttat ttcgaaaaac ccattgccgg atactggatt aacagcattg
    23101 ggcaatggct atttggcgcg aataactttg gtgtgcgggc aggcgttatc tttgcgaccc
    23161 tgttaactgc cgcgctggtg acctggttta ctctgcgctt atggcgcgat aaacgtctgg
    23221 ctctactcgc cacagtaatt tatctctcat tgtttattgt ctatgccatc ggcacttatg
    23281 ccgtgctcga tccgtttatt gcattctggc tggtggcggg aatgtgcagc ttctggctgg
    23341 caatgcaggc acagacgtgg aaaggcaaaa gcgcaggatt tttactgctg ggaatcacct
    23401 gcggcatggg ggtgatgacc aaaggttttc tcgcccttgc cgtgccggta ttaagcgtgc
    23461 tgccatgggt agcgacgcaa aaacgctgga aagatctctt tatttacggc tggctggcgg
    23521 ttatcagttg cgtactgacg gttctcccct ggggactggc gatagcgcag cgggagcctg
    23581 acttctggca ctattttttc tgggttgagc atattcaacg ctttgcacta gatgatgccc
    23641 aacatagagc tccgttctgg tactacgtgc cggtcatcat tgccggtagc ctgccgtggc
    23701 tgggattact ccccggtgca ctgtacacag gctggaaaaa ccgcaagcat tccgcaaccg
    23761 tctatttgtt gagctggacg ataatgccgc tgctgttttt ctccgtcgct aaaggcaaat
    23821 tgcccaccta tattctttct tgctttgcac ctctggcaat gctgttggcg cattacgcgt
    23881 tgctggcagc aaaaaataat cctctggcgc tgcggattaa tggctggatt aacatcgctt
    23941 ttggcgtcac tggcattatt gccacatttg tgatctcccc ctggggacca atgaacacgc
    24001 cggtgtggca aaccttcgag agctataaag tcttttgtgc ctggtcgatt ttttcgctat
    24061 gggcattttt cggctggtac accttaacaa acgtcgaaaa gacctggtct ttcgccgcgc
    24121 tttgcccgct ggggctggcg ttgctggtag gattttcaat tcctgacaga gtcatggaag
    24181 gaaaacatcc gcaatttttt gtcgagatga cacaagaatc acttcagcca agccgctata
    24241 ttcttactga tagcgtcggt gttgccgcag gtctggcatg gagcctgcaa cgcgatgaca
    24301 tcatcatgta tcgccagaca ggtgagttga aatacggcct taattatccg gatgcgaaag
    24361 ggagatttgt cagcggtgat gagttcgcaa actggcttaa tcaacatcgt caggagggga
    24421 ttattacact cgtgctttcg gttgaccgcg atgaagatat caacagtctc gccattccgc
    24481 ccgcagatgc catcgttcgt caggagcgtc tggtgctgat tcagtatcgt cccaaatgat
    24541 ctggctaaca ttagtctttg ccagcttgct tagcgttgcc gggcagttgt gtcagaaaca
    24601 ggcaacctgc tttgtggcga taaacaaacg gcgcaaacat atcgtgctgt ggctgggact
    24661 ggcgctggct tgtcttggtc ttgccatgat gctctggctg ctggtcttgc agaacgtacc
    24721 ggtaggcatt gcctacccga tgttaagtct gaattttgtc tgggtgacgc tggctgcagt
    24781 aaaactgtgg cacgaaccgg tatcgccgcg tcactggtgt ggggtggcgt tcattattgg
    24841 tggcattgtg atcctcggga gtacggtgta atgggcctga tatggggatt attcagcgtc
    24901 attattgcct cagttgcgca attaagcctg ggttttgcgg caagtcatct gccgccgatg
    24961 acgcacttat gggattttat cgcggctctg ttagcttttg gcctggatgc ccggatactg
    25021 ttactcgggc tgctgggtta tctgctttcc gttttttgtt ggtataaaac gctacataaa
    25081 cttgcgctaa gcaaagccta tgccttgctc agtatgagtt atgtgctggt atggattgct
    25141 tcaatggtac ttcccggctg ggaagggact ttttcgctga aagcactact gggagtagcc
    25201 tgtattatga gcgggttgat gctgattttc ctgcccacga caaaacaacg ttactgagtt
    25261 ttccctgccg ctgtacactg ccgttcccac tcatcggggc tataactgct tgccgagagg
    25321 actttcaccg tatgcagctt ttcacgatta atacaataaa gcgcattctg caaaggcgag
    25381 agtaaatccc ccactttcac ggcgaagtca ctcttcactt cggcgatcat ttttatcgcg
    25441 ccgccagcat cgcagagcat aagtacatgt ctgttatctt gtttattgca gcacgatttt
    25501 ttgtgccagc cattccattg cattatcctg tttgctaaga gttttcacat caatttacag
    25561 attaacagat aaactcttta tcaacggcaa tggaatggca gaaatgagaa gtgaaacgga
    25621 gggttaatta ttgctgacgt tgcacccact cttttagcgc ctgacgtgaa attttaatac
    25681 cgccgttttt cagctccggc ggtaaagtca gccagcgcac cggttgttga aaacgagcca
    25741 gcttatcttt cacccattcg ccaagatcaa cggtttgctg gtcatactcc accaccgcta
    25801 ccggccgatg accaaactcc ttgtcggcaa cggaaacgat aaacacctgc aaaaccgcag
    25861 gatgtgcagc aataacacgc tcgacttctt ccggctgaat accctcaccg ccactgaaga
    25921 acagattgtc taaacgtccg acaatggtca gcttgccatt atgcatctca ccgcgatcgc
    25981 gcgtagcgta ccagccttcg tcattaacca gtgaaaccag ttgcccgtta cgccagtaac
    26041 cttctgccat actggcagcc cgcagccaca cttcattatt aacgattttc acttcccgac
    26101 ccggcagcgg caaaccaacg tctgccaggc cgtcggcttc tttcgcacat accgtggagg
    26161 caaactcggt cagaccatag ccgcaaaagc aacgaatccc ctgctcgcgc gcctgttccg
    26221 tcaactcgac cgggatagcc gcgccgccaa gtaacaccgc tttcagggaa acggaactgc
    26281 ggttaaccag caaacgccag agttgtgttg gcaccagtga agcgtgagta cagcctgcca
    26341 gcatttgctc caatggctgt ttatcacgca ccgtcatccg cgcaccagcg tataaccagc
    26401 gccacataat tccctgaccg gagacgtgaa acagcggtaa agagagcaac cagtcatcgt
    26461 gatcgccaaa cggaatcagc gataacacac cttgcgcact ggcaagatgg gcctgataag
    26521 tatggacagc ggctttcggc aaaccggtag aacccgaggt caacgtcatt gagcacagac
    26581 gcgtcggctg ccacgcagcg gcatgtacgc cttcaaccag ctgaatgtgc agcgacgtta
    26641 atgccggaaa cgtgttttcc ccatccggca ccagcgcaaa ttgcagcgtc agattgggca
    26701 gcaattcttc aagcaacggt tgcggcagct gagggttcac gggcaacacc cgcgccccgc
    26761 attgcagtaa cgccagccag gcgagcagcg tttgcggcgt attccacgcc cgcaacatca
    26821 cgccgctgcc ctcaaccacc ccctgcgccg caaagcccgt agctaactca tcgacgcgag
    26881 cacaaagctc gcgccagttg agttgctcgt cattaagacg taaggcgatg gcttctcccc
    26941 gcacttgccg ccagtgacgc cacggccagt cagagaagat cataacaact gctcctgtac
    27001 atcagcgtcc actaacggca gtgggctccc cggccagcga cgaacctgct gcgcctgcat
    27061 cagatccagc gtgtccagtc ccggaatggt gtctggcgtt aaccaggcag caatccgcgc
    27121 cagttgcgtt agacctaagc tcgactcaat ggaagaactg atcaccgccg tcagccccag
    27181 cgcatgcgcc gcctgtacct gctcgcgtac tttttccaga ctgcccgtga gcgtgggttt
    27241 gataactacc gcgcgcacgc cctcttcagc cacaaaggca aaatccggct cgcgcaggct
    27301 ttcatcccag gcaatggcaa tgccggtttc acgggcaaac gctcgcgaat catcgcgggt
    27361 tttgcacggc tcttcgagaa acgcgatgcg gtggcgataa tccgggttaa catatttggc
    27421 aaactgctga cctttcagcg gtgtccaggc gcgatttgcg tcaagacgca aatgcagatc
    27481 cggaattgcc tccagcaaca gattcaccac catgccgtcg cgcaccgctt cgtacaatcc
    27541 cactttgacc ttcgccactt tctcgcccgg catatcggca agtttgagga tcagatcgtc
    27601 cggatcgcca ttacacagcg gtgccgcacg gtagttggct gcttgcggca acgtatctgt
    27661 cagttctgcc aatgcacagc ttacgccaaa ggccacggaa ggcatctgcg gtagctcgca
    27721 atcgcctgcc aaccagttat ttacccaggc aagcagcacg ctttgcgcct cttcccagtt
    27781 ttcctggctg aagcccggca gtggggagat ctccccccac ccttcgcgct cgccctcacg
    27841 caggcaaacg tacagcccgt cgcgggtttt taaccgcctg tcgcgcagaa ccacccccgc
    27901 gtccatgggg atctgccagc ggtatacctg cgcgctacgc attacggatt ccgtttaaat
    27961 ttgctgaaat caggctgacg tttctggttg aaagcgttgc gaccttcctg accttcttcc
    28021 gtcatgtaga acagcatggt ggcgttgccc gccagctcct gcagccccgc ctgcccgtca
    28081 cagtcggcgt tcagcgcagc tttcaggcag cgcagcgcca tcgggctgtt ttgcagcatt
    28141 tcgcggcacc aacggacggt ttctttttcc agatccgcca gcggtaccac ggtgttgaca
    28201 aggcccatat ccagcgcctg ttttgcgtcg tactgacggc acaggaacca gatttctcgc
    28261 gcttttttct gcccgacgat acgagccatg taggaagcgc cccagccgcc gtcgaaggaa
    28321 ccgactttcg ggccagtctg accgaagatg gcattatctg ctgcgatagt caggtcgcac
    28381 atcatgtgca gaacgtgacc accgccgatg gagtagccag ccaccatcgc gacaaccggt
    28441 ttcggacagg tacggatctg acgctggaag tccagcacgt tcaggtgatg tacgccggaa
    28501 tcatctttat agccgccgta atcaccacgc actttctggt caccaccgga gcagaacgct
    28561 ttatcgcctg cgccagtcag aatgatcacg ccgatattgt cgtcataacg cgcatctgcc
    28621 agtgcctgga tcatctcttt taccgtcaga ggacggaagg cattgcgcac ctgcggacga
    28681 ttaatggtga tttttgcgat accgtcggtg gatttttcat aacgaatgtc ctcgaaacct
    28741 tcggagcagt cgtgccattc aaccggtgcg taaggcattg cttcatcagg ataaatcata
    28801 ttgtgtcctt tagtcagaaa cgcaagatct gcgccagact tgcgattacg ccagcgggat
    28861 tttcccgatg cgcgttatgt ccggcgcgag gaatgacatg gcagtcggca gccagttccg
    28921 ccgccagggc gcggaatttg ctgtcacgtt caccacataa ataataaaac gcaaatgtgc
    28981 gggcgctaag gttagcacgt aaatcaggct ggacggcgag agaagtcgcc tccagcatgg
    29041 cggcaagggt tgcgccatta ttgttgctgc gcagcgccac cagctcccgg cgttgatcgt
    29101 cattgagcga ggcaaaaaca ggctgttgat accagtcggc aaataccgcc gttaacggtt
    29161 ctgtgcgaaa acgctgcgcc cattggcgat cggaacgctg acgttccgca cgttgttcag
    29221 cattttgcag ccccggatgc ccgccttcga caataacccc acaaagcccc gccagcccct
    29281 ggcaagccgc catcatcgcc acccgtccgc caagcgagta ccccaccagc cagaagtcaa
    29341 ggatgttgta actaaccaag gttttacgca gtaagtcggt gacatcatca aatccatcga
    29401 cgctaatcgc cgccgaacca ccatgaccta ggagatcaac atacaaccgt gagtagtcgg
    29461 caaacgcctc gcccacttct tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca
    29521 ccagccaggg taaacctagt tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct
    29581 tacctgcgcc agaagttgct ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc
    29641 aatcaccgtg gtggttggcg tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca
    29701 gttttgcgga tgatgatatt tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt
    29761 ttgcggcatc agatagaaac gctcacgctc gctttgcggc gttggcaaca gcgagaaaat
    29821 ttgcccgccg ttgttgttca ccacaattaa taccagcggc gcagaaacct gacgcaataa
    29881 cgccagcgcg ttgagatcgt aaagcgcgga gagatcgccc acaatcgcca gcgtcggttt
    29941 gccgcttgcc cgctgaacgc ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc
    30001 tcggttgctg tacaccgggt aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac
    30061 caccaggctg ttaccaacaa acaattgccc ctgttcaggc agatagtcgc tgatgcgatg
    30121 cgccagttgc gcttcgccaa acgcatcacg gcgggcaata accgcctgca ttgcctgttc
    30181 cgccaggcgc gggatttcaa cgcaccaggg ctggcgtttt tctgccggat gcagctccag
    30241 ccagtcggca atattggcaa ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc
    30301 ttcaatgtca tcaacaatcc agtactcttc tggttcacag cttgcctgcc attgcaggag
    30361 ccgtttcccc gtcaggctac ttcccagttg caccacaatt tgcgcctgct gcagctcgct
    30421 ggtcgctttg gcattgccta accaaagatc ggcacacggc agcggctgcc cggtttgtga
    30481 cagcacatcg ccaatcagcg gccagccaag agtttgcgcc cacagggcaa cttttttgcc
    30541 ctcttccgca ctcatgcgcc cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca
    30601 gtcgcgctgt ttttcacttt ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg
    30661 ccaccagtca cccagacgct gttgccagct aagcccggta tcgtccattt cgccatacag
    30721 cggttcagca aacgggcagt tgatatggac tccccccgca tgaagcgtac cgagagcgtg
    30781 gtcgatggta gaaaccagcc aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat
    30841 actgtgcgtg gggtgagagg cgaacattcc cggctggcga attgcctgat tcgcgccgca
    30901 gtcaattagc tccggcgggc gatcggcggt taagagaatc agtttttctc cggttaaccc
    30961 ggcttcaatc agtgccggat agagatttgc caccgccgtg ccggaggtca caatcaccgc
    31021 caccggctgc ttgctgactt tcgccagccc cagcgccaga tgccccaacc cacgctcatc
    31081 gaaatgggtg tggtgaatga atgcggaatt ctccgccgcc gctaacgtta acggtgtaga
    31141 acgcgagcct ggggcgatac agatgtgtct gacgccgtga cgcgttaatg cttccagaat
    31201 gaccgccgcc cagcgtcggt taaatgcgct tactgacatg agtttgtccg gtatcaatat
    31261 tgcggctaag tataaggagc tacaaaaata gattattgat atgaatcggt aatgatgcga
    31321 ctcattacta ttccatttgt aataaagtac gcagccctgc cgctttgttg tcgatttcct
    31381 gccactcttg ctcggggtcg gaaccacgga caatgcccgc gccagcatat aatcgcacga
    31441 cattgccgct aatttttgct gagcgcaggg aaacgcagaa ttcgctttgt tgtaatgaga
    31501 gatagcccgc agaaccggcg taccattctc gggtgaacgg ttcgtgacgg gcgataaact
    31561 gtcgcgccag atcgcgcggt aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac
    31621 agatcacatc atccgctttg ttgagtgaag tccagataca gcggcgaaga tgctgcactt
    31681 tacgcagacg cagtacctgc ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc
    31741 gttggcagat atcttccacc accagcatgt tctcgcgctg gtttttatca tccgccatca
    31801 gccactctcc taactgctgc gcctgcttat catcaggatg atttgctact gttcccgcca
    31861 gcgcttcagt acgcagcgct ttgtcacgcc gccgccataa ccgttccggt gaagagccaa
    31921 gaaaagcatt ttcgccatca aaggccatgt aaaaatggta gcaattcaga ttcagtcgac
    31981 gactggcagc catcatcgcc gccgcgttga ccggacttgc gaaatgcagg tcagttgccc
    32041 gagcgagcac cactttgtcg agctcacctt cggcgatggt tttcgttgcc agttcgatta
    32101 attgcgtcca gcccgttttg tccggccagt gttgttctcg cgtggtggtt aaatgtaacc
    32161 caggcaaggg cttgatactc accagtgtgg cgataaattc ttttgcctga atcgcatcgt
    32221 gctgaaggga gctttcgctg aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc
    32281 gccattccag gcggggtaaa agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc
    32341 aaatgcgtaa gtcagcgtgt tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg
    32401 acgtaaaacg ggtaatcgcg cccaggacgg cagcttcttc atcaccatta cgttgttgcc
    32461 agtagaattg cgggtacgtt tgctgactgg ccagccagct caaggcatca aaagcgtctt
    32521 tgagagggaa aggaatatcg ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca
    32581 aatggcgcag tagattttcc agcgccgtag taagtgattg cacgcggacc tctccctgtt
    32641 aaaagcctca cattatacgg ggtactacaa aaaaaatgca gtaccccggt gtagggaggt
    32701 tttaacggcg tgccagcaac agtcctagta ccagcccgac ggccgcgccc acaccaattc
    32761 cttgccaggg tttttcgtgg acgtagtcat cagcacgata aacagcctgc ttcgcccgat
    32821 agtaataact gtctgaagcc tggctaaccc gttttttcac atcatccagc gctttttctg
    32881 catgcgcttt cagctcaaca tatttctgat cggcgggatc gccagaggag cggagcacct
    32941 cttccagtgt ttcactaagc agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat
    33001 tagacattct cgttctccat ttgcgtaaaa cctgtcacta actatagaca agagttgttg
    33061 attacgcctg aattacctcg cgtgccatcc caatgtgtgg gataccatct tcttcataga
    33121 cctccgtcac agggataaaa ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc
    33181 cgaggtaaac aggcttatca ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca
    33241 gctgctgccc cactttttca ccacgcaacg cttcactgac aatcacccga cctataacga
    33301 ccggctcaag atcatcatca cttttcagaa tcctcgcata cgccaccagt tcatcatttt
    33361 tccaaccgag aatatggcga ttatcccccg tcaaatcatc accatcgata tcctgataag
    33421 ggcaattctg ctcgacaaca aacaccgcgc aacgcagttg taataaggca taaagctggg
    33481 aaaccgataa ttcagagtga tgcagatctt gccattcaat cataatgctc tccttttaga
    33541 tgtatgcctt atactagccg gaatattttt tgaaacgtga tgaactcatg gaattaattt
    33601 ttttaggtac ttcagccggt gtgccaaccc gcacgcgcaa tgtcacggca atattgctta
    33661 acctgcaaca tccgacccag agcggacttt ggttgtttga ctgcggtgaa ggcacccagc
    33721 atcagctact gcataccgcc tttaaccctg gaaaactgga caagattttt atcagtcacc
    33781 ttcatggcga tcatcttttt ggtttacccg gcttgctgtg cagtcgttct atgtcaggca
    33841 ttatccaacc cttaacgatt tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc
    33901 tgcggattag cggctcatgg accgattatc cgctggaaat tgtcgaaatt ggcgctggcg
    33961 aaattctcga tgatggcctg cgcaaagtaa ccgcttatcc gctggaacac ccactggaat
    34021 gttatggcta tcgtattgaa gaacatgata aaccgggtgc attaaatgcc caggcattaa
    34081 aagctgctgg cgtgccgcct ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca
    34141 cgctggaaga tggaaggcag attaacggcg cagattactt agctgctcca gtgccaggta
    34201 aagcgctcgc tattttcggc gataccggcc cctgcgatgc cgcacttgac ctggctaaag
    34261 gtgtcgatgt catggtgcac gaagcgacgc tggatataac catggaagcc aaagccaata
    34321 gtcgcggcca tagctctaca cgccaggctg cgacactagc ccgtgaggct ggagtcggca
    34381 agctaatcat tacccacgtc agctcgcgct atgatgacaa aggttgtcaa cacctgttac
    34441 gtgaatgcag gtcaattttc ccggcgactg aactggcgaa tgatttcacc gtgtttaacg
    34501 tttaactctg cttattatta aaaacagggt gaaacttgcc ctgttatagc aacccgcgct
    34561 acatatatcc acccacacag gcatattgag cgatttttga tatttattca tctccttacg
    34621 cactatattc agagcaataa gttgcctctt ttcatcttga ttcagcacaa cctttgcaac
    34681 taaaaggagc ccatgatggt tacagttgtc agcaattatt gtcaattatc tcaaacccaa
    34741 ctcagtcaga catttgcaga aaaatttact gtgaccgagg aattactgca gtctttaaaa
    34801 aaaacagcgt tatccggaga tgaagaaagt attgagttac tgcataatat tgcgttaggt
    34861 tatgatgaat ttggaaaaaa agctgggtaa tgctgccaac ttactgattt agtg
//
LOCUS       DAJTRB010000036        34367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTRB010000036 DAJTRB010000000
VERSION     DAJTRB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.036"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..332)
                     /locus_tag="OO330_001860"
     CDS             complement(<1..332)
                     /locus_tag="OO330_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882746.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OO330_001861"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OO330_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5882747.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OO330_001862"
     CDS             complement(1693..2073)
                     /locus_tag="OO330_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5882748.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OO330_001863"
     CDS             complement(2087..2503)
                     /locus_tag="OO330_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882749.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO330_001864"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO330_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5882750.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OO330_001865"
     CDS             complement(3504..3971)
                     /locus_tag="OO330_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5882751.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OO330_001866"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OO330_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5882752.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDASAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OO330_001867"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OO330_001867"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5882753.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OO330_001868"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OO330_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5882754.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO330_001869"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO330_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5882755.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OO330_001870"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OO330_001870"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5882756.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OO330_001871"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OO330_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5882757.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OO330_001872"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OO330_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5882758.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO330_001873"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO330_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5882759.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OO330_001874"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OO330_001874"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5882760.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OO330_001875"
     CDS             complement(14626..15441)
                     /locus_tag="OO330_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5882761.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OO330_001876"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OO330_001876"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5882762.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OO330_001877"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OO330_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5882763.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OO330_001878"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OO330_001878"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5882764.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO330_001879"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO330_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5882765.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OO330_001880"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OO330_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5882766.1"
                     /translation="MTLGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEARFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OO330_001881"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OO330_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5882767.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OO330_001882"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OO330_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5882768.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OO330_001883"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OO330_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5882769.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OO330_001884"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OO330_001884"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5882770.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO330_001885"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO330_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5882771.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OO330_001886"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OO330_001886"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5882772.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OO330_001887"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OO330_001887"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5882773.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO330_001888"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO330_001888"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5882774.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO330_001889"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO330_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5882775.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERSKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OO330_001890"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OO330_001890"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5882776.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OO330_001891"
     CDS             31575..32189
                     /locus_tag="OO330_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5882777.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OO330_001892"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OO330_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5882778.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OO330_001893"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OO330_001893"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5882779.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8081 a         8683 c         9284 g         8319 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctagctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgtc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgca gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgctaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgagg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtacca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggctaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgctgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcctag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttc
//
LOCUS       DAJTRB010000037        34243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTRB010000037 DAJTRB010000000
VERSION     DAJTRB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.037"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..568
                     /locus_tag="OO330_001894"
     CDS             <1..568
                     /locus_tag="OO330_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5882780.1"
                     /translation="AFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYASQRY
                     LEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSA
                     LELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRVQYV
                     LDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(565..1701)
                     /locus_tag="OO330_001895"
     CDS             complement(565..1701)
                     /locus_tag="OO330_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5882781.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1707..2030)
                     /locus_tag="OO330_001896"
     CDS             complement(1707..2030)
                     /locus_tag="OO330_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5882782.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2213..2356
                     /locus_tag="OO330_001897"
     CDS             2213..2356
                     /locus_tag="OO330_001897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882783.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2575..4113)
                     /gene="aldB"
                     /locus_tag="OO330_001898"
     CDS             complement(2575..4113)
                     /gene="aldB"
                     /locus_tag="OO330_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5882784.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4278..5327)
                     /gene="yiaY"
                     /locus_tag="OO330_001899"
     CDS             complement(4278..5327)
                     /gene="yiaY"
                     /locus_tag="OO330_001899"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5882785.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVKEEDFAVLATNALKDACGFTNPI
                     QATHEEIVVIYRAAM"
     gene            complement(5517..7361)
                     /gene="selB"
                     /locus_tag="OO330_001900"
     CDS             complement(5517..7361)
                     /gene="selB"
                     /locus_tag="OO330_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5882786.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7358..8749)
                     /gene="selA"
                     /locus_tag="OO330_001901"
     CDS             complement(7358..8749)
                     /gene="selA"
                     /locus_tag="OO330_001901"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5882787.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEAIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8847..9455)
                     /locus_tag="OO330_001902"
     CDS             complement(8847..9455)
                     /locus_tag="OO330_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5882788.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9526..10611)
                     /locus_tag="OO330_001903"
     CDS             complement(9526..10611)
                     /locus_tag="OO330_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5882789.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10614..10976)
                     /locus_tag="OO330_001904"
     CDS             complement(10614..10976)
                     /locus_tag="OO330_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5882790.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11513..13426
                     /gene="mtlA"
                     /locus_tag="OO330_001905"
     CDS             11513..13426
                     /gene="mtlA"
                     /locus_tag="OO330_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5882791.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHIRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13521..14669
                     /gene="mtlD"
                     /locus_tag="OO330_001906"
     CDS             13521..14669
                     /gene="mtlD"
                     /locus_tag="OO330_001906"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5882792.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14669..15256
                     /gene="mtlR"
                     /locus_tag="OO330_001907"
     CDS             14669..15256
                     /gene="mtlR"
                     /locus_tag="OO330_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5882793.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15268..15477)
                     /locus_tag="OO330_001908"
     CDS             complement(15268..15477)
                     /locus_tag="OO330_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882794.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15761..16123
                     /locus_tag="OO330_001909"
     CDS             15761..16123
                     /locus_tag="OO330_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5882795.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16667..17350
                     /locus_tag="OO330_001910"
     CDS             16667..17350
                     /locus_tag="OO330_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5882796.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17394..22056
                     /locus_tag="OO330_001911"
                     /pseudo
     CDS             17394..22056
                     /locus_tag="OO330_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22424..24079
                     /gene="lldP"
                     /locus_tag="OO330_001912"
     CDS             22424..24079
                     /gene="lldP"
                     /locus_tag="OO330_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5882797.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            24079..24855
                     /gene="lldR"
                     /locus_tag="OO330_001913"
     CDS             24079..24855
                     /gene="lldR"
                     /locus_tag="OO330_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5882798.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24852..26042
                     /gene="lldD"
                     /locus_tag="OO330_001914"
     CDS             24852..26042
                     /gene="lldD"
                     /locus_tag="OO330_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5882799.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26238..26711
                     /gene="trmL"
                     /locus_tag="OO330_001915"
     CDS             26238..26711
                     /gene="trmL"
                     /locus_tag="OO330_001915"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5882800.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26764..27585)
                     /gene="cysE"
                     /locus_tag="OO330_001916"
     CDS             complement(26764..27585)
                     /gene="cysE"
                     /locus_tag="OO330_001916"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5882801.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27665..28684)
                     /gene="gpsA"
                     /locus_tag="OO330_001917"
     CDS             complement(27665..28684)
                     /gene="gpsA"
                     /locus_tag="OO330_001917"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5882802.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28684..29151)
                     /gene="secB"
                     /locus_tag="OO330_001918"
     CDS             complement(28684..29151)
                     /gene="secB"
                     /locus_tag="OO330_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5882803.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29214..29465)
                     /gene="grxC"
                     /locus_tag="OO330_001919"
     CDS             complement(29214..29465)
                     /gene="grxC"
                     /locus_tag="OO330_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5882804.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29607..30038)
                     /locus_tag="OO330_001920"
     CDS             complement(29607..30038)
                     /locus_tag="OO330_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5882805.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30283..31827
                     /gene="gpmM"
                     /locus_tag="OO330_001921"
                     /gene_synonym="pgmI"
     CDS             30283..31827
                     /gene="gpmM"
                     /locus_tag="OO330_001921"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5882806.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31837..33120
                     /gene="envC"
                     /locus_tag="OO330_001922"
     CDS             31837..33120
                     /gene="envC"
                     /locus_tag="OO330_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5882807.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            33124..34083
                     /locus_tag="OO330_001923"
     CDS             33124..34083
                     /locus_tag="OO330_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5882808.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(34070..34243)
                     /locus_tag="OO330_001924"
                     /pseudo
     CDS             complement(34070..34243)
                     /locus_tag="OO330_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8520 a         8974 c         8730 g         8019 t
ORIGIN      
        1 tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca gctacgctcc
       61 accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa ccgcttatgc
      121 cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg aacatcactc
      181 ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct tcaacgtcgc
      241 cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg ataacacgca
      301 atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag acaaaagtgt
      361 gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt atgaatggtg
      421 gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg tccagtatgt
      481 gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc aactgcggcc
      541 tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca cccagctggt
      601 catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag agtagaccgc
      661 cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat ccagttcaat
      721 ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg tgccggtcga
      781 ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc tgaatacctt
      841 acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc gcagcaagga
      901 gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca ccatcaccgg
      961 acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg tcacataacc
     1021 atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg cgagctgcgc
     1081 tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct ggctctggat
     1141 ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat aattttgccg
     1201 cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc cgcgtgcgta
     1261 acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag tattcgccgc
     1321 catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa cgatatccgc
     1381 catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata gcacctcacc
     1441 ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg tcacctgtgg
     1501 gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat ggttatagtt
     1561 cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg tggaaatggt
     1621 ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag ccacataggt
     1681 caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat gtcggcgagc
     1741 tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc atggatttgc
     1801 attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa tggccagata
     1861 acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc atgaggatga
     1921 ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc atagaatatc
     1981 accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat atttccctca
     2041 aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca gtgcaatttc
     2101 cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct gtctggtttt
     2161 tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac taatgaataa
     2221 ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta tctaccgttt
     2281 attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc cgctcaccat
     2341 cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc tgttggcgta
     2401 gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc gctttgttta
     2461 tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta ggcatgataa
     2521 gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct tatatcagaa
     2581 cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct ggtaatgctc
     2641 cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac caaacgcagc
     2701 atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta tgccgcgccc
     2761 catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc catattgcgt
     2821 atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg ccagcaccgg
     2881 gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa tcgtcggttc
     2941 gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc ctgtgagcac
     3001 gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca gttgcccgtg
     3061 agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc gaatgctttc
     3121 gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca ctaaggcacg
     3181 actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc cttccagcgc
     3241 tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat ttggcgactt
     3301 accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca taatttgttg
     3361 gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg tcgccagata
     3421 ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg gcagtaaatc
     3481 accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg gtttcagcac
     3541 cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca gcagcgggaa
     3601 gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat aggccacggt
     3661 ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg cgaaatagcg
     3721 gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg gtttgccgtt
     3781 atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat cggcaatctt
     3841 aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt tcactttgtg
     3901 cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt cgcacagcag
     3961 ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg ctacccattc
     4021 accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat actcgccggg
     4081 cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt gtgaggtata
     4141 acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta cgacagctat
     4201 cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca atttgccagc
     4261 cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc ttcgtgagtt
     4321 gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc cagaaccgcg
     4381 aaatcttctt ctttcacgtt cacgcccatt gcagcggcac agtcacgcag acgtgcagcg
     4441 gcgactttgc tgttgaatac ctgaacatgc ggcagcagaa cggcgttaca tacaccgtgt
     4501 ggcaggttgt agaaaccgcc cagctggtgc gccatcgcat gaacataacc cagagaagca
     4561 ttattgaacg ccataccggc aaggaactgg gcataagcca ttgcttcacg cgctttcgca
     4621 ttactgccat cttcaacggc taacggcagg ttttcggcaa tcatggtcac ggctttcagt
     4681 gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat agcgtgcgtt
     4741 aaggcatcca tacccgttgc ggcggtcagt gacttcggca taccaatcat cagagaggag
     4801 tcattgacag aaagcagcgg agtcacgtgc ttatcaacaa tcgccatttt gttatgacgc
     4861 gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc cgtggtattg
     4921 atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcgta atcgcgaata
     4981 tcgccgccat tggctgccac cagcgcaata ccttttgcgc agtcgtgtgg agaaccaccg
     5041 cctaaggaga tcacgctatc gcaattattt tctttaagta atttcaaacc tgcggcgacg
     5101 ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat attgcgttct
     5161 tccagtgctt tttgcacatc acccgccata cctaatttcg ttaacatatt gtcagtgaca
     5221 attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcagt caatgaatca
     5281 gcgccgatga cattcacaga aggaataaag aaagttgaag atgccataac acactcctga
     5341 aagtgtaaaa aagggtgggc agcttactga ggattttcat tcgaaaacat gatcattatt
     5401 attaattatc aaaattaatc taataaaaag cacattgtat tcattaaaca atgtgcttta
     5461 tttaaaatca taagaaattt gtattaattt attttaaaaa ttaattaatc atttccttat
     5521 ttttccggaa ataataatgc gtcgcgcaat aaatgatcat ttccacgacg acgcgtaaag
     5581 ccaatgcggt caaaatattc cagaatctga attgccagct ttcggcctac gcctaagcga
     5641 tcgcggaaat ccgccgcgca ggttgaacca cactcctgat cgagatcgcg gatcatattg
     5701 gcaaattcga caatccgatc gttacggtaa taacgatctt tgacgatcgc ggtaattatt
     5761 ccttgctgcg ccgcctggcg tagcgtcagg cgcattgcct gctcgtcggt acccgtctct
     5821 tttgccagat cacgcaccca ccacggctcg tcaccaaaca gcggctctgc tttttgccag
     5881 atggcctgct gctcttcgct gaagcccgct ttgtgatccg gcagatgcag ccaaccgtga
     5941 tgactgagga tgtcgccgct ttcgcgcatc ttttcaatca gcaacagtac tagcgcttca
     6001 tcttccattg gcaacgccat acgtcgcaga cgttcgcgcc caggcccagg ttcatcgcga
     6061 tgttgctcat gataagtcgc taatgtgtcg agaattttcc gttgccagcg agcggcaacc
     6121 ggcgcattca acaagctata accagcctga atatagccag gctgttgcag cagttcgcgc
     6181 atcccttcgc cgttgagctg gcgcgcccag gcgaaatcag gaagattaac cgcgccgcgt
     6241 tgcaggtgaa cggctaacgc ttcgctatca ttctgtgccc tggcgaggga agccagccat
     6301 tgcagatact ctggtttacg tttaccgcga cgcggcgggt taagcatcac gacgcgcgct
     6361 ccggccagcg tgttgcgggc agagatatcg cgcaatacca ggcggtcgtt atctgccagc
     6421 cataacgggg tgtcgaagac cagttcagca aggttatctt ccagcagtga aacgcgtccc
     6481 gtgacgtggc tggcggcgtg gtgaatatgc agcggctgcc actgggtcag cggtgcatgg
     6541 gtttgaagct cgacaatcac ccgtgtgaac ggctctggcg gcacatcggc aagcagccag
     6601 tcgccacggt taatctgctc tttttccgca tcacccgcga tgtcaagcgc gatacgctgc
     6661 ccggcattgg cggtttctgt tggctggttt tgcgcatgca gcgcacgtac acgcatcggt
     6721 ttatttacac cagtcagcca gagtgaatcg cctaccttca cttccccgct taacgccgta
     6781 ccggtgacga ccagcccggc accttttacg gtaaatgcgc ggtcaatcgc gaggcggaaa
     6841 ctatgttggc tggcgtgctc gcgttccggc aactgaagca gatgctcgcg cagggcatca
     6901 attccccgac cttcggttgc tgcggtgata aacagttttg cctcagcaaa accgtattcc
     6961 cgcagaacct cctttacctg gcgttcaacc tcatcaacac gcgcttcgtc cacgcgatcg
     7021 gctttggtca gcgccactgt cagcatcggg ttaccggtca gctgcaaaat cgccagatgc
     7081 tcacgggtct gtgccatcac gccgtcatcg cacgccacca ccaacagcgc gtgatcgata
     7141 ccaccaacgc ccgccagcat gttggaaaga aacttttcat gaccgggaac gtcgataaaa
     7201 ccaggcacgc gaccatccgg ctgcggccag taggcatagc cgagatcgat ggtcatgccg
     7261 cgcttttttt cttccggcag acggtcagca tttacgccag taatcgcctg caataaggtt
     7321 gttttgccgt ggtcaacgtg tccggcagtc gcaataatca tttcaacaac atctccaaaa
     7381 accgttgctc atcttcaagg cagcgtaaat ccagccacaa tcgtccgtca taaatacgac
     7441 caatcaccgg cactggcaat tcacgccagc gggcggctaa tgactcaagg tggctaccgc
     7501 gtccatcatg gggtgtaaac gttaatgccg cgctcggcag gcgatcaacc ggcagcgaac
     7561 cactgccaat ctgcgaaaga catggcataa cctgtaccgc aaactccgcg ccgtaatgtg
     7621 cgacaagggg agcctgtaaa cgttgtgcct ggatttgaat ggcctctgcg ctgcgggtaa
     7681 gcaggcgcag ggtcggtaat ttttcactca gagcttcagg gtgtaaataa agacgcaacg
     7741 tggcttccag cgccgcgagg gtcattttat ccgcgcgtaa tgcacgcttc agcgggtggc
     7801 tttgcaggcg ggcgatcatc tcttttttac caacgataat cccggcctgc ggcccgccta
     7861 acaacttgtc gccggagaag ctcaccagac tgacgcccgc cgcaatcaac tcctgcggca
     7921 ttggctcttt cggcaaaccg tactggctaa gatcgaccag cgagccactg cctaaatcag
     7981 tcaccactgg aacatccagc tctttgccga gcgccaccag ttcagcttcg tctatcgctt
     8041 tggtgaaccc ctgaatgctg tagttactgg tatgtacttt catcaacagt gcggtatttt
     8101 cattcaccgc ctgacgataa tcattcgcgt gcgtgcggtt ggtggtccct acttcgtgta
     8161 gggtgcagcc tgcctgacgc ataacatcgg gaatacgaaa cgcgccgcca atctccacca
     8221 gttcgccgcg agataccacc acctctttcc cgctggcagt ggccgccaac atcaataaca
     8281 ccgccgccgc attgttattg acgatacagg catcttccgc ccccgtaata cggcacagca
     8341 gctgcgccag cgcccgatcg cgatgtccgc gtccggcgtc gtccagatcg tactcgaggg
     8401 tcactggcga acgcatagcc tgcgcaacgg cttccaccgc ggcttccgcc tgtaaagctc
     8461 gcccaaggtt ggtatgcagc acggttcccg tcaggttgat caccggacgc agcgcgctct
     8521 gcgcctcttt cgtcaaccgg gcatcgactt cttgcgccca gttttcacac cacgcaggca
     8581 gcgtctggct gccacgaatc acttctcgcg cttcgtcgag catctgacgc aacaattcca
     8641 ccacgcgggt gtgaccataa gtatcacgca aagaaaggaa ggagctatcg cgcaataagc
     8701 gatcaatagc cggaagttga ctatagaggg aacgcgtttc ggttgtcata ggaaacctgg
     8761 ctgatcaagg ccctctcaca cggagaaggg cgtttaacat aaccacggat tgtaacgtga
     8821 gatgggtcag gaggacatat cgcgcatcaa gcctttggcg gttcggtgcg agcaaaactt
     8881 tcgcggctaa acaggttttc gaccagtttg actaagtgtg ggcggtcgac acaccaacct
     8941 ggcgcaacgc ggcggaaatt gagatatccg acagcacagg caatggcgat agtcgccaga
     9001 ttgaccgtat cggttttgag tgtgccatcg accagatatc cttccagcac atccagactg
     9061 cggttgattt tctcccgctg gcgtaacaat tcatcttcag actgctgcgc cgccggacgc
     9121 gcctgttcac gcaccgatac cagcccggca tccataatgc catccgccag cgcctcaatt
     9181 ttgcgcaccc gcaacgactc cagcggatcg cgcggcaaca tcgccggagc gacattcatt
     9241 aactcaatat attcagcgat gatcggcgaa tcaaaccagc attcgccctc ttcggtcacc
     9301 agcaccggca ctttccctaa cgggttaaat tgcgccacgc cgttatccgc gttatagggc
     9361 agttcattaa taaattcgaa agttatgccc ttttctaaca acagaataga aagtttgcgt
     9421 acaaacgggc tggtgtagct accgacgagt ttcatgccga gtcctttgtg cgaggaaaaa
     9481 tatcagtatg gctcaggctc acgcaaaaca caggtgatat aagtctcaat gatccaaata
     9541 aagataatgc atccaactgg tcattcttag cagcactttc cgcatcaccg aaacatggct
     9601 gaaatggtcg gagtaaaccg ccacctgggc gtagatgccg tcgggtaagg catcgatatc
     9661 atcgttaggg tccagttcaa tggttcccag cacaccgtcc gtgccgggaa cgaccgttaa
     9721 tgattgcaat accccctgcg cctgataaga accgcctggc acgacaggta aaatactagt
     9781 caatttgccg tggaacacct gcccaggtag cgcgttaaac accacttccg catcatcgcc
     9841 aggtttcaga cgtaacagcg agttttgccg aaattgggcg acaatttgcc gtttttgctc
     9901 ggggatgaag accatcaccg gacgcagcgg caaggcagct gcgtatgtac ctgggcggat
     9961 cagtacctga gtgacgtagc cattgcttgg tgcgcgaatg acagtctgct caagattata
    10021 ttttgcttca gtaagttgcg ctcttaagct cacaatctgc gattgctcgc cgttgaccat
    10081 actgtcgagc tgactctgga tctgcgcctg ctccgccacc gagcctttca ctaacgcatc
    10141 ctgcgcgagg aaattttgcc gcgcatcgtc gatgtcacgt tccgagaacg gattcaccgc
    10201 cgcctggctg cctttcaggt aacgttgata atttttaaac agacggtcgc gctccgctga
    10261 aacctgggtg gtgttggcct gcgcttcagt gagctgcgca cgcagcgtct ttatattatg
    10321 cgtcgccgtc atcaggtcag cctgaagtct gtcaactcgc gcctggtaac gaaccgggtc
    10381 gagcttaaaa agcacctcgc ccttttgaat aagctgatta ttcttgtcag tgacttcagt
    10441 aacaattccc gtcacctgtg gcgtgatagg gatcgctatc actgcctttt gcgcggtaaa
    10501 agtgtaaggg tggttgtagt tcatcaacaa aatcaaacca ctcaccagaa acacgcctcc
    10561 cagcgccgcc gtcgccagcg ccacgtaagt taaaacaatc aatagatcca taattagatc
    10621 tccgggaacg tggaatagtc agtctttttc tcggcggcga gttgatgctc cagccgggaa
    10681 attcggtcag aaagtgcggc gatttccggt tcagtcgctt tctcctgcga cgcaacatgt
    10741 gactgcatac cccagccacg ctccggttga tagagcgtcg cccagatcca cagaaacggc
    10801 cagataacat gcagagtaaa caggctcacc cagcccgccg tatgaatagc gtcggcatgg
    10861 gggtgattgc gctttttggc aatcagataa gggatgtcat gtatcgcgat gattccataa
    10921 aaaatcacca gaaagacgaa gatcagcact cccaacgcga aatagtttag aaacatatct
    10981 gcctcggatt cacgtttatc agtgttgttt tttggctggc agccagaagg gagtcaggct
    11041 gatattttga caataatccg ggttcgcgat tctcgccata acaccaaaga ataattttta
    11101 gaggtgatga gttgcttagt tacataacga ttgtatgacg aaggcataac atgctgtaga
    11161 tcacatcagg tgaacgccgt aagaaaatat cttgtgattc agatcacaaa gattcaacaa
    11221 accatcaaaa caaaaatgtg acattactca catttaaatg ccatttttag cgaaaatcac
    11281 cgccttgttt cttttttata caggcgtttt gtgatgagcg tcacgtcaat tacctctcta
    11341 ccccctatat ttatgtgact gatatcacac aaaagcccgt cgactggaca gttaaccgat
    11401 tcagtgccag atttcgcagt atctacaagg tccggctacc tctgccgcca cattaacaaa
    11461 aaacctcggg cttccagcct gcgcgacagc aaacataaga aggggtgttt ttatgtcatc
    11521 cgatattaag atcaaagtgc aaagctttgg tcgtttcctc agcaacatgg tgatgccaaa
    11581 tatcggcgcg tttatcgcgt ggggtatcat caccgcgtta tttattccaa cagggtggtt
    11641 accgaacgag acgctggcga agctggtcgg gccgatgatc acttatctcc taccgctgct
    11701 gatcggttat accggtggta agctggtagg cggcaaacgt ggtggcgtag tcggtgccat
    11761 taccaccatg ggcgttatcg tcggcgcaga catgccgatg ttcctcggtt ctatgattgc
    11821 aggtccgctg ggcggctggt gcattaagca cttcgaccgc tgggtagacg gtaagatcaa
    11881 atccggtttt gagatgctgg tgaataactt ctccgcaggc atcatcggga tgatcctcgc
    11941 tattctggca ttcctcggca ttggcccgat tgttgaagcc ctgtccaaaa tgctggctgc
    12001 gggcgttaac ttcatggttg tccatgacat gctgccgctg gcgtctatct ttgttgaacc
    12061 ggcgaaaatc ctgttcctca acaacgccat taaccacggt atcttctcgc cgctgggtat
    12121 tcagcagtcc catgaactgg gtaaatcaat cttcttcctg attgaagcta acccaggtcc
    12181 aggtatgggc gtgctgctgg cgtacatgtt ctttggtcgt ggtagcgcta aacagtctgc
    12241 gggcggtgcg gcaatcatcc acttcctggg tggtatccac gaaatctact tcccgtatgt
    12301 gctgatgaat ccgcgtctga tcctcgcagt catcctcggc ggtatgactg gcgtgttcac
    12361 gctgactatc ctgggcggtg gtctggtttc tccggcatct ccgggttcta tccttgctgt
    12421 actggcgatg acaccaaaag gtgcttattt cgctaacatc gcgggtgtgt gtgcggcgat
    12481 ggctgtctcc ttcgttgtct ctgctatttt gctgaaaacc agcaaagtga aagaagaaga
    12541 tgatattgaa gcagcaactc gtcgtatgca ggacatgaaa gctgagtcta aaggcacatc
    12601 tccgctatct gcgggcgatg tgactaacga cctgagccac atacgtaaaa tcatcgttgc
    12661 ctgtgacgcc ggtatgggtt caagtgcgat gggcgcaggc gtgctgcgta agaaaattca
    12721 ggatgcaggt ctgtcgcaga tttcggtcac taacagcgcg atcaacaacc tgccgccaga
    12781 tgtggacctc gtcatcactc accgtgacct gaccgaacgc gctatgcgcc aggttccgca
    12841 ggcacagcat atttcgctga ccaacttcct cgacagcggc ctgtacacca gcctaaccga
    12901 acgtctcgtt gctgcccagc gccatactga aaacgaagtg aaagtgaaag acagtctgaa
    12961 agacagcttt gacgattcca gtgctaacct gttcaaactg ggcgcggaga acatcttcct
    13021 cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt gctggcgagc agctggtgaa
    13081 aggcggttac gttgagccgg aatacgttca ggcgatgctg gatcgtgaaa aactgacccc
    13141 gacttatctg ggtgagtcta tcgcggtgcc acacggtacg gttgaagcga aagatcgcgt
    13201 actgaaaacg ggcgtggtgt tctgccagta cccggaaggc gtgcgcttcg gtgaagaaga
    13261 agatgacatt gcccgtctgg tgattggtat tgcagcccgt aacaacgagc acattcaggt
    13321 tatcaccagc ctgaccaatg cactggatga tgagtccgtc atcgagcgtc tggcacacac
    13381 caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt aagtaatcca atcctaccct
    13441 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
    13501 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
    13561 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
    13621 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gtcggtgaaa
    13681 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
    13741 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
    13801 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
    13861 cgctgaacat catcgcctgt gaaaacatgg tacgcggcac cacgcagctg aaaggtcatg
    13921 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agagcacgtt ggctttgtcg
    13981 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
    14041 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
    14101 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
    14161 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
    14221 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
    14281 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
    14341 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
    14401 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ctgctcggta
    14461 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
    14521 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
    14581 cgcaggcggc gctggcacag atttccggtc ttgatgccaa cagcgaggtt gtatccgagg
    14641 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
    14701 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggccg ttgaaaaccg
    14761 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
    14821 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
    14881 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
    14941 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
    15001 actgctgatg gcattgcgtg aagagctaaa tcacgacggc aacgagtacg cctttaccga
    15061 cgacgaaatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
    15121 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
    15181 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccaaaatca gcttaaaaaa
    15241 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
    15301 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
    15361 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
    15421 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
    15481 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
    15541 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
    15601 tatctttttc tatagatttc ttaattaatc aacgaattgc gtagtttttg agcatgttaa
    15661 tgattacgtt gcagaactat tacaatgtca tcagttgtga acaaagcacc tggtcgcgca
    15721 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
    15781 cgaaatcaag cgtctcagcg atcgcctgga cgccatccgc caccagcagg ccgatctgtc
    15841 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
    15901 agaaattgct cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag cacagaagct
    15961 gatgaagatg ccgttccagc gcgcaattac caaaaaagag caggctgata tgggcaagct
    16021 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg ggcgcgaaat
    16081 gggcctggaa gagatgactg gattttctaa gactacgttt taagcccctc agacgactgg
    16141 ttcgatcgta tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
    16201 ctactcgcct aattaccaaa caattcactc cctttacttc gatttaccgc tggttgatat
    16261 tctgaaaatg catttttacc gaaatagcca tctataaaca ataaaaatat atagaagata
    16321 tcatttgtta attaacaagc atttacgtct ctttttttcg gatttaattg tgaaatttta
    16381 atcatcatat tcacaaaatc aattatctgt gaaatttttc ccagacatgg tttctttcat
    16441 atttgacatt tattaataat catcattaga atccacgaaa aataatatat tcatacaaac
    16501 acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa
    16561 tcgacacaca tcactgtcat taatgtaaag caacatagct caccaataat acaatggaca
    16621 actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga acttgaagaa
    16681 gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat
    16741 tgaagaaggc aagccagaaa gtagtttact gaaacaacaa gaagaacata ataataaaat
    16801 tgtgctactt gaaaaacaac aagcacagct aaagagtcaa ctcgaaacta ttcaaaaaca
    16861 acaaactggc ataataaaca gtacaaagac tttaactcac gtaattaaat ccgttaaaga
    16921 ccaacaaaat acttttattt ttaccgagtt taacccagca aaaacgaagt atttcatcct
    16981 gaacaacggt tcggtggctt tagcgggtcg agtgttatcc attgacgcca cagagaatgg
    17041 tagtgttatt catatctcac tggtcaactt attaagtaca cctatctcaa acattggttt
    17101 taatgcgaca tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc ggtggcaaca
    17161 attacttttc aacacatcaa tgaaatccac attgaaatta ttaccaggtc aatggcaaga
    17221 cattaatttg accctgaagg gtgtatcgcc caataacctg ggatatctga agttagccat
    17281 caacatggaa aatattcagt tcgacaatct tccctctgct gaaaatcggc agaaaagaag
    17341 caaaaaataa atgcagttag tacgcttata acaaggaata gcgagcatca ttaatgaaca
    17401 aaatatttaa agttatctgg aaccctgcga cagggaatta tactgttacc agcgaaacgg
    17461 caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg ctggttgcag
    17521 gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac ggtcagggtg
    17581 ttgattacgg tagtggatca gctggcgacg gctgggttgc tataggcaaa ggggcgaaag
    17641 caaatacttt tatgaacacc agtggttcca gtactgctgt gggttatgac gctatagctg
    17701 aaggccaata tagctctgcc atcgggtcaa aaacccatgc gattggtggt gcatcaatgg
    17761 cctttggggt tagtgcaata tcagaaggcg atagaagtat agcactgggt gcctcttcgt
    17821 attcattggg ccaatactca atggccctcg gccgttattc aaaagcattg ggtaaattgt
    17881 ctattgctat gggggactct tccaaagcgg aaggagcaaa cgccattgcc ctggggcgta
    17941 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
    18001 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
    18061 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
    18121 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
    18181 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
    18241 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
    18301 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
    18361 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
    18421 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
    18481 tttatgccat tagcgactcg gtagcaaaaa aggcttggag gaggggctgc agtagatgtt
    18541 gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta
    18601 ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa
    18661 tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat
    18721 ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat
    18781 gacgatgtcg aagccaacac caccaatatc accaacctga cggattccgt tggtgacctt
    18841 caggctgatg ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag
    18901 gataccacca gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat
    18961 gctgttaacg gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat
    19021 atcgccaata acacttccaa tattgccact aacaccacca acatctctaa cctgactgag
    19081 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg
    19141 gcagctcatg gcaccgagac caccagcaaa ataaccaacg ttaaagatgg cgacctgacg
    19201 actggcagca ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg
    19261 acgaatacca ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg
    19321 actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct
    19381 catggcaaca ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc
    19441 agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac
    19501 ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc
    19561 ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc
    19621 acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac
    19681 ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc
    19741 agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg
    19801 ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt
    19861 gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcctgac
    19921 gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga
    19981 aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc
    20041 agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg
    20101 ggtggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg
    20161 aacgccgagt acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa cgccctgctg
    20221 tgggataaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc
    20281 agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac
    20341 ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag
    20401 ctcgctggta acaccgacgc aacctatatc gaagaaaatg gcgctggtat caactacgtt
    20461 cgtactaacg acaacgattt agcctttaac gatgcaagcg cctctggtgt cggcgctaca
    20521 gctgtaggtt ataacgctgt cgcgtctggt gccagcagcg tagccattgg tcagaacagc
    20581 agcagcaccg ttgataccgg tattgcgctg ggtagcagct ccgtttccag ccgtgtgatt
    20641 gccaaaggtt ctcgtgacac tagcgtaacg gaaaatggcg tggtgattgg ttatgacacc
    20701 actgacggcg aactgctagg tgcattgtca attggtgatg acggtaaata ccgccaaatc
    20761 atcaacgtag ctgatggttc agaagcccat gacgccgtta cggttcgcca gttgcagaac
    20821 gctattggag cggtcgccac tacgccaacc aagtactttc acgccaactc aacggcagaa
    20881 gactcactgg ccgttggtga agactcgctg gcaatgggtg cgaaaactgt cgttaatggt
    20941 aatgcaggta ttggtatcgg tttgaacact ctggttctgg ctgatgcgat caacggcatt
    21001 gctatcggca gtaacgcacg cgcaaaccat gcaaacagta tcgcaatggg taacggttct
    21061 cagaccaccc gtggtgcaca gactggctac accgcctaca acatggacgc accgcagaac
    21121 tctgtaggtg agttttctgt cggtagtgaa gacggtcaac gtcagatcac aaacgtcgca
    21181 gctggttcag cggataccga tgcggttaac gtgggtcagt tgaaagtcac tgatgagcgc
    21241 gtagcgcaaa atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact
    21301 cgcgttacta atatcgaaaa cggtattggc gacattgtca ccaccggtag caccaagtac
    21361 ttcaagacca acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt
    21421 ggttctggtt ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca
    21481 gaggaagaaa atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta
    21541 gctgccagtg ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca
    21601 ggcggagtgc gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc
    21661 ggtggtggca acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac
    21721 gacgcggtga actacgcgca gttgaagcaa agcgcgcagg aaacgaagca atacaccgat
    21781 cagcggatgg ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt
    21841 atcgcttctg caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg
    21901 gcttctattg gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg
    21961 gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa
    22021 tactccgccg cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta
    22081 agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgctccggg
    22141 tcaacaatct aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc
    22201 cccacaagaa ttggccctac caattcttcg cttatctgac ctctggttca caattcccca
    22261 attaaaactc acatcaatat tgccaataca taacatttag ttaaccattc attgtcatta
    22321 tccctacaca acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca
    22381 acagactcat tacacgatgt gcgtggactc taggagacct gcaatgaatc tctggcaaca
    22441 aaactacgat cccgccggga atatctggct ttccagtctg atagcatcgc ttcccatcct
    22501 gtttttcttc tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac
    22561 ggtagcaatc gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct
    22621 ggcctcggtg gtttatggct tcttctatgg tttgtggccc atcgcgtgga tcattattgc
    22681 agcagtgttc gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc
    22741 tattctttcg ataacccctg accagcgtct gcaaatgctg atcgtcggtt tctgtttcgg
    22801 cgctttcctt gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct
    22861 ggtcggcctg ggttttaaac cgctgtacgc cgccggactg tgcctgattg ttaacaccgc
    22921 gccagtggca tttggcgcga tgggcattcc aatcctggtt gccggacaag taacaggcat
    22981 cgacagcttt gagattggcc agatggtggg gcgtcagcta ccgtttatga ccattatcgt
    23041 gctgttctgg atcatggcga ttatggacgg ctggcgcggt atcaaagaga cgtggcctgc
    23101 ggtcgtggtt gcaggcggct cgtttgccat cgctcagtac cttagctcta acttcattgg
    23161 gccggagctg ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct
    23221 caaacgctgg caaccagttc gcgtattccg cttcggtgac ttgggggcgt cacaggttga
    23281 tatgacgctg gcccacaccg gttacaccgc gggtcaggtg ttacgtgcct ggacaccgtt
    23341 cctgttcctg accgctaccg taacactgtg gagcatcccg ccgtttaaag ccctgttcgc
    23401 atcgggtggt gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcaataaact
    23461 ggttgcccgt atgccaccag tggtcagcga ggctacagcc tatgccgccg tgtttaagtt
    23521 tgactggttc tctgccaccg gcaccgccat tctgtttgct gcactgctct cgattgtctg
    23581 gctgaagatg aaaccgtctg acgctatcag caccttcggc agcacgctga aagaactggc
    23641 tctacccatc tactccatcg gcatggtgct ggcgttcgcc tttatctcga actattccgg
    23701 actgtcatca acactggcac tggcactggc gcacaccggt catgcattca ccttcttctc
    23761 gccgttcctc ggctggctgg gggtattcct gaccgggtcg gatacctcat ctaacgccct
    23821 gttcgccgcg ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt
    23881 tgccgccaat accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat
    23941 cgcctgtgcg gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa
    24001 acacagcctg atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt
    24061 aacgtggatg attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat
    24121 cgtgtgcggg cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgag
    24181 cgccaactgg cgatgcaact cggcgtatca cgtaattcac tacgcgaggc gctggcaaaa
    24241 ctggtgagcg aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt
    24301 catgacacat ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat
    24361 ccggattaca gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg
    24421 catgcggcaa tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca
    24481 acgctaagtg aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt
    24541 gccgaagcct cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg
    24601 caatcctcag tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa
    24661 ctgaccgaac aacatcaggc tgtcattgac gccatttttg ctggtgatgc cgacggggcg
    24721 cgtaaagcaa tgatggcgca ccttagtttt gttcacacca ccatgaaacg attcgatgaa
    24781 gatcaggctc gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg
    24841 gagaaaaacg catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc
    24901 tgccgccgtt cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc
    24961 gcaacgtgga agatttgtca gaagtggcac tgcgccagcg tattctgaaa aacatgtccg
    25021 acttaagcct ggaaacgacg ctgtttaatg agaaattgtc gatgccggta gcactggcac
    25081 cggtgggttt gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg
    25141 acgcgcatgg cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg
    25201 cgccagccat caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta
    25261 tgcgtaacgc gctggagcga gcaaaagcag cgggttgttc gacgctggtt ttcaccgtgg
    25321 atatgccgac accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccaaatg
    25381 cggcaatgcg ccgctacttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc
    25441 tgaacggtcg tccacatgat ttaggtaata tctcagctta tctcggcaaa ccgaccggac
    25501 tggaagatta catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc
    25561 ttgagtggat ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg
    25621 aagatgcgcg cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg
    25681 gccgccagct ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt gcagatgcgg
    25741 tgaaaggtga tatcgccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc
    25801 gtatgattgc gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg
    25861 caacagcggg ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag
    25921 tggcgatgac gctgactggc gcgaaatcga tcagtgaaat tacgcaagat tcgctggtgc
    25981 aggggctggg taaagagttg cctacggcac tggctccgat ggcgaaaggg aatgcggcat
    26041 agtcgtttgc gccccctcac cctaaccctc tccctcaggg agaggggacc gttcggcgct
    26101 gtatgtactc cctcactctg aaacgacacc gcactctttt tttctccctc gcccctccgg
    26161 ggagggccgg ggtgagggga aaaggccgca cggttcacca attttctgct atcctgcccc
    26221 cgcactaagg gggcagtatg ctaaacatcg tactttacga accagaaatt ccgccaaata
    26281 ctggcaacat catccgtctt tgcgctaata ccggctttcg tctacatatc atcgaaccga
    26341 tgggatttgc ctgggacgac aagcgcctgc gccgcgcggg gctggactat cacgagttta
    26401 ccgccgttac gcgtcatcat gactatcgcg cgttcctcga agcagaaaat ccccagcgcc
    26461 tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc cgtaagctat caggatggcg
    26521 attatctgat gttcggcccg gaaacacgcg gcctgccagc gagcattctt gatgccctgc
    26581 ccgctgaaca aaaaattcgc attccgatgg tgccggacag ccgcagcatg aatctgtcca
    26641 atgcggtgtc ggtagtggtg tatgaagcct ggcggcagct gggatatccg ggagcgttgt
    26701 tgagagatta gttactgtat gccggataga tgattacatc gcatccggca cgatcacagg
    26761 acattagatc ccatccccat actcaaatgt atggttaata ccgttgaaat gctggtccat
    26821 atccattgat ggcttatcgc tgtctggttt accgacaata cgaaccggaa cgccagcggc
    26881 ggttgtatgc ggcggcaccg gttgcagcac cacggaacct gcgccaatct tcgcgccgcg
    26941 accaacttca atattgccga ggattttcgc gcccgcgcca atcatcacac cttcacgaat
    27001 tttcgggtga cggtcaccac cagatttacc cgtaccgcca agcgtcacag attgcagaat
    27061 cgatacgtcg ttttcaatta ccgccgtttc acccacaacg atgcccgtgg cgtggtcgag
    27121 cataatgcca cggccaattt ttgctgccgg gtgaatatcg acctggaacg tcacagaaac
    27181 ctggttttgc agaaagattg ccaatgcgcg acgcccctga ttccacaacc agtgaccgat
    27241 gcgataggcc tgcaaggcat gaaaaccctt caggtataac aacggggttg agtatttatc
    27301 gactgccggg tcgcgggtac gcaccgcctg aatatcacag gccgcagagg cgatcatttc
    27361 cgggtcagcg gcgtaggctt cttcaaccac ttcacggata gcaatagcag gcataattgg
    27421 cgatgacagc ttgttcgcca gcatgtagct cagtgcactg ccaaggtttt cgtgcttgag
    27481 tagcgtcgcg tggtaaaaac tggccagcat tggctcacag tccgccagcg ttctggcttc
    27541 ggctttaata ttgttccaga caatttccag ttcttcacac gacattgctt actccacacg
    27601 atgagataat gaccggcccg ttctgcgcgg gccgggtcat agcggtaaca aaggttccct
    27661 ggggttagtg actgctgcgc tcgtccttgc gtgcacgacc taataatgtc aatgctgcct
    27721 cgcgcgcgtt ttttccgcaa tataatactt gataaatttc ctcggttatt ggcatttcaa
    27781 cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt gcggtagcct tccaccacct
    27841 gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg accgagcatc ataccaaaac
    27901 ggcggttacg cgactggttg tcggtacagg taagcaccag atcgcccagc cccgccatgc
    27961 ccataaaggt ggcaggatcg gcacccagcg ccgcaccaag acgcgacatt tcagccagcc
    28021 cacgggtgat cagcgccgta cgcgcattcg caccaaaacc gataccgtca gacatccccg
    28081 cgccaatggc aatgacgttt ttcaccgcgc cgccaagctg cacgccaatg aaatccgggt
    28141 tgctgtaaac gcggaaactt ttgccgcagt gcagcagctg ctggagatca tcggcaaagg
    28201 tctgatcggt cgaggccagc gaaatagctg tcggtaaacc tgccgccagt tctttcgcaa
    28261 acgttgggcc agagataacc gccagcggaa tttgatcgcc taaggcctca cgcgccacgt
    28321 cctgtaacag acgtccggtt tccgcttcca gccctttggt cgcccacacc agacgcgcat
    28381 caggacgcat cagcggttta atctggcgca gcacttcacc aaagacatgg ctgggtacga
    28441 cgacgagaat attacgactg gctgccagcg cagtggcgag atcgctttca agatggagcg
    28501 tatcgggaaa aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt tcaagcgttg
    28561 cgatatgttc agggtcatgg ccccagagga caacctcgtg gccatttctt gccagggtaa
    28621 tggcaagagc ggtgccgtac gagccggcac cgatcacagt cattgaagca ttacgttggt
    28681 tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca aatagttcat
    28741 gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc gggaatgtac cgcgggatac
    28801 catgctggtg atgcactcac gagcatacgg gaacagaatg ttcgggcagt atgcgcccag
    28861 gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag aaaataccgc cctgctgaac
    28921 ttcacacagg aacgcggttt cttcgcccaa ggaggccgtt acggtaacac gcagtaccac
    28981 ttcgtatacg tcatctgcca gttgggaaga tgccgtatcc agatcaagtt taacttctgg
    29041 ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaga tatccttggt
    29101 ataaatacgt tggatctgga aagtcatttc agtgttgttt tgttctgaca tgtgtagaaa
    29161 acccttaagt gttgtcctta aatactgcgc aatgtaatac caacgttcac acgttatttc
    29221 agcaggggat ccagtccacc acgtgcatcc aatgcataca agtcatcaca gccgccaatg
    29281 tgctgtgcgt caataaaaat ctggggaacc gtggtgcaac cgctgcgttt gatcatctct
    29341 tcacgcttgg cggcattgcc atcgatcggc aactcctgga aactcacgcc cttgctgctc
    29401 agcagtgctt ttgcacgatg gcaatacggg caggtttctt tggtatagat ttcaacattg
    29461 gccataattt agctcctgtt tttttaccct gaaatttcat gttgcagggc ggctgcaaaa
    29521 tggagtgtcc ccagaagctt ataacaataa gtcgctgggg gcctaattgc agctaacggc
    29581 ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca ccagaggcaa gttttcgcca
    29641 gcccagccag caacgccctc tttcagcacg aatacttgcg caaaaccagc tttcgtcagc
    29701 gcgtttgcag gctcctggca ctgcatgcca gaaccgtcta ccacgataac cgatttgtct
    29761 ttgtgcttct caagctcacc aacattgttg gctttgattt cgctcggcaa cagattgata
    29821 gaacctgcga tatggccttt acggaagtca tcacgctgac gtaaatccac aaccacagcg
    29881 tcttctttgt tgatcagacg cgtagcttca ccacgagtaa tcaccttcac tttcgaggtc
    29941 aggctcttaa acgtagtcac aagaaccgcc accagtaacg cgatccaggc gatactcagt
    30001 atgggatgac ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa ctcccgacgt
    30061 agtgattaaa aaaaccagga aaggagtata cctgcgcggt gcggcaaata cagccagcgc
    30121 gttaactgga atgcaatttt gcggggcacg acgaaaaaaa agcgcaaatc tggcatcggc
    30181 ttgccgcgag cggtcgtata ttttgatctt cagaggctat tttatcgatt cagctgtagt
    30241 aaaattacgc aaattttgac tcttgagtat gaggttgtcg caatgtcggt ttctaaaaaa
    30301 cctatggtac tggtgattct ggatggctat ggctatcgtg aagaacagca ggataacgcc
    30361 atttttagtg ctaaaacccc ggtaatggat gcactgtggg ccaatcgtcc gcataccctg
    30421 atcgacgctt ccggtctgga agtcggtctg cctgaccgtc agatgggtaa ctccgaagta
    30481 ggccacgtta acctgggtgc cggccgcatc gtgtatcagg acctgactcg tctggacgtt
    30541 gaaatcaaag atcgcgcttt ctttgctaat ccagtgctgg ctggcgcggt acataaagcg
    30601 aaaaacgcag gtaaagcagt acacattatg ggtctgctct ccgcaggcgg cgtacacagc
    30661 cacgaagatc acatcatggc gatggtagaa ctggcagctg aacgcggcgc agaaaaaatc
    30721 tacctgcacg cattccttga cggtcgcgac accccgccgc gcagtgctga atcctcgctg
    30781 aaaaaatttg aagagaaatt tgctgcgctg ggtaaaggcc gcgtagcgtc catcattggt
    30841 cgttactacg cgatggaccg cgataaccgt tgggatcgcg ttgaaaaagc ctatgacctg
    30901 ctgactctgg cgcagggtga gttccaggcc gatactgccg ttgctggttt gcaggcagct
    30961 tatgctcgcg acgaaaacga cgagttcgtg aaagcgaccg ttattcgtgc agaaggtcag
    31021 ccagatgcgg caatggaaga cggcgatgcg ctgattttca tgaacttccg tgctgaccgc
    31081 gcgcgtgaaa tcactcgtgc attcgtgaac gctgatttcg atggcttcgc gcgtaagaaa
    31141 gtggttaacg tcgatttcgt gatgctgacc gaatacgccg ctgacatcaa aactgcggtt
    31201 gcttacccac ccgcttccct ggttaacacc ttcggcgagt ggatggcgaa aaacgacaaa
    31261 actcagttgc gtatttccga aaccgaaaaa tatgcccacg ttactttctt cttcaacggt
    31321 ggcgtagaag agtcgttcaa aggcgaagat cgcattctga tcaactcacc gaaagtggct
    31381 acctacgatc tgcaaccgga aatgagctcc gcagagctga ccgaaaaact ggttgcggcc
    31441 atcaagagcg gcaaatacga caccattatc tgtaactatc cgaacggcga catggtaggt
    31501 cacaccgggg tgatggaagc ggcggttaaa gcggttgaag cactggatca ctgcgtggaa
    31561 gaagtcgcga aagcggttga gtccgtgggt ggacaactgc tgatcaccgc tgaccacggt
    31621 aacgctgaac agatgcgcga tccggcaacg ggtcaggcac acacggcaca caccaacctg
    31681 ccagttccgc tgatttacgt tggtgataag aacgtgaaag cggttgcagg cggcaaactt
    31741 tctgacatcg cgccgaccat gttgtcgctg atgggtatgg aaatcccgca agagatgact
    31801 ggtaagccgc tgttcatcgt ggaataatcc ctccccatga ggggaaaggc gattaatacc
    31861 atgacacggg ccgtgaaacc gcgcaggttt gcaatcaggc ccatcatcta cgccagcgtt
    31921 cttagcgctg gcgtattgtt gtgcgccttt tccgcccacg cggatgagcg tgaccaactc
    31981 aaatctattc aggccgatat cgccgcaaaa gagcgcgcgg tacgccaaaa gcaacaacaa
    32041 cgcgcaagcc tgctcgcaca attgaaaaag caggaagaag cgatctctga agccacccgt
    32101 aagctgcgcg aaacgcaaaa cacgctcaat caactcaata aacagattga tgagatgaac
    32161 gcgtcgattg ccaaactgga gcagcaaaaa gccgcccagg agcgcagcct cgccgcacaa
    32221 ctggatgccg cattccgtca gggcgagcat accggtattc agctaattct cagcggtgaa
    32281 gaaagccagc gtggacagcg tttacaggct tatttcggct atctcaacca ggcgcgacaa
    32341 gaaaccattg cccagttgaa gcaaacgcgt gaagaagtca ccatgcagcg tgctgaactg
    32401 gaagagaaac aaagcgagca acaaacgctg ttatatgaac agcgcgccca acaggcgaaa
    32461 ctgactcagg ctctgaacga gcgtaaaaag acgctggcgg ggctggaatc ttccatccag
    32521 caaggtcagc agcagctaag tgaactgcgt gccaacgaat cccgtctgcg taacagcatt
    32581 gcccgtgcgg aagccgcggc gaaagcgcgt gctgaacgag aagcgcgtga ggcccaggcg
    32641 gttcgcgagc gccagaaaga agcgacgcgc aaaggcacca cctacaaacc gaccgaaagc
    32701 gaaaaatcgc tgatgtcccg aactggtggc ctgggtgcgc cgcgtggtca ggcattctgg
    32761 ccggttcgcg ggccgacgct gcatcgctat ggcgagcagc tacagggtga attacgctgg
    32821 aaagggatgg ttatcggtgc ctctgaaggt actgaagtta aagcaattgc cgacggtcga
    32881 gtgattctgg ctgactggct gcaaggttac ggtctggtgg tggtggttga gcacggtaaa
    32941 ggcgacatga gtctttacgg ctataatcag agcgcactgg tgagcgttgg ttcgcaggtt
    33001 cgcgcgggcc agccaattgc actggtgggc agcagtggcg gtcagggtcg gccttcactc
    33061 tatttcgaaa ttcgccgcca gggtcaggcg gtcaatccac agccgtggtt gggaagataa
    33121 gttttgtttc catttcgtcg taacgttctt gcatttgccg ctctgttggc gctctcctcc
    33181 cccgtacttg ctggcaaact tgccatcgtc attgatgatt ttgggtatcg cccgcacaac
    33241 gaaaaccagg tgctggcgat gccttccgct atctccgtcg ctgtattacc cgattcaccg
    33301 cacgccagag agatggcgac caaagcgcat aacagcggtc acgaagtgtt gattcatctc
    33361 ccgatggcac cgttgagtaa acagccgctg gagaaaaata cgctacgccc ggagatgagc
    33421 agcgacgaaa ttgagcgcat tattcgtagt gcggtcaata acgtgcccta tgccgtgggg
    33481 atcaacaacc acatgggcag caagatgacc tctaacctgt ttggtatgca gaaagtgatg
    33541 caggcgctgg agcgttacaa tctttacttc ctcgacagcg taaccatcgg taatacccag
    33601 gcgatgcgcg ccgcgcaagg tactggcgtg aaggtgatca aacggaaggt gttcctcgac
    33661 gattcgcaga acgaagcgga catccgtgtg caatttaatc gcgcaattga tctggcgcgt
    33721 cgcaacggtt caaccattgc cattgggcat cctcaccctt caacggtacg cgtgctacaa
    33781 cagatggttt ataacctgcc gccagacatt acgctggtga aagccagcag cttgctgaat
    33841 gaaccgcagg ttgatacttc tacaccgccg aaaaacgctg tgcctgatgc accgcgtaat
    33901 ccgttccgtg gcgtgaagct gtgcaaaccg aagaaaccga tagaacctgt ttatgctaat
    33961 cgcttctttg aagtattaag cgaaagcatc agccagagca cgctgatcgt ttacttccag
    34021 catcagtggc aaggctgggg caaacagccc gaagcggcga agtttaacgc tagcgcaaat
    34081 taaaggcgct acgcgtaatg cgatggtgcg accgcgtttt gtcgcgccat tgccataagc
    34141 ggaaagacca cagtaacgcc tggtaaccga ccttcacgct gcgcacattg gtaatcaggc
    34201 gcttatacat acccggaagt aaatatctcg gcaatcatcc gtt
//
LOCUS       DAJTRB010000038        33528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTRB010000038 DAJTRB010000000
VERSION     DAJTRB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.038"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO330_001925"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO330_001925"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5882809.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO330_001926"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO330_001926"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5882810.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO330_001927"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO330_001927"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5882811.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO330_001928"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO330_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5882812.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO330_001929"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO330_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5882813.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO330_001930"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO330_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5882814.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO330_001931"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO330_001931"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5882815.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO330_001932"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO330_001932"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5882816.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO330_001933"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO330_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5882817.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO330_001934"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO330_001934"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5882818.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO330_001935"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO330_001935"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5882819.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO330_001936"
     CDS             8212..9075
                     /locus_tag="OO330_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5882820.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO330_001937"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO330_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5882821.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSITQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO330_001938"
     CDS             10411..11028
                     /locus_tag="OO330_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5882822.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO330_001939"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO330_001939"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5882823.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO330_001940"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO330_001940"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5882824.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO330_001941"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO330_001941"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5882825.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO330_001942"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO330_001942"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5882826.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO330_001943"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO330_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5882827.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO330_001944"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO330_001944"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5882828.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO330_001945"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO330_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5882829.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO330_001946"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO330_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5882830.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO330_001947"
     CDS             21859..23568
                     /locus_tag="OO330_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5882831.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO330_001948"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO330_001948"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5882832.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO330_001949"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO330_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO330_001950"
     tRNA            27625..27719
                     /locus_tag="OO330_001950"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28019..29203
                     /locus_tag="OO330_001951"
     CDS             28019..29203
                     /locus_tag="OO330_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5882833.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29615..30658
                     /locus_tag="OO330_001952"
     CDS             29615..30658
                     /locus_tag="OO330_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5882834.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31173..31601
                     /locus_tag="OO330_001953"
                     /pseudo
     CDS             31173..31601
                     /locus_tag="OO330_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31631..32782)
                     /locus_tag="OO330_001954"
                     /pseudo
     CDS             complement(31631..32782)
                     /locus_tag="OO330_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32847..33501
                     /locus_tag="OO330_001955"
                     /pseudo
     CDS             32847..33501
                     /locus_tag="OO330_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8399 a         8814 c         8261 g         8054 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tagtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct ctggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcac
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacgatatcg aacatcatta cccactggat agatgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacc gatatcaaag tgaaaaccgc aaaacccaaa gacaaaccct ataagcttgc
    28081 tgatggcggc ggtatgtatc tactgattaa cactaatggt tctaaatact ggcgcatgaa
    28141 gtaccgcttt gctggtaaag aaaagatgct ttctattggc gtataccccg atgtaacgct
    28201 agccgatgct cgcgagaaac gcagtgaagc caggaaactc ctcgctgctg gaggcgatcc
    28261 aggggaggca aaaaaggaag aaaaaatcgc gcagcaaatg agtctgaaaa atacgttcga
    28321 ggctattgct cgcgaatggc accagttaaa agctgatcgg tggtcattac gctatcgtga
    28381 tgaaatcatt gatacctttg aaaaagacat ttttccctac attggcaagc gccctattgc
    28441 cgaaatcaaa ccaatgaagt tactggaagc actgcgtaaa atggaaaaac gtggagctct
    28501 ggagaaaatg cgcaaggtcc gccagcgctg tggtgaggtg ttccgctatg caattgttac
    28561 tggccgggca gactataacc cagcccccga tcttgccagt gctttagcta caccgaaaaa
    28621 agtacatttt ccctttctta ctgctaatga acttccccac ttcctcaacg atctggcggg
    28681 ttataccgga agtataatca ctaagacagc tactcagatc attatgttaa ccggcgtacg
    28741 aacacaagaa ctgcgttttg cacgctggga agatatcgat tttgagacaa aattgtggga
    28801 gatcccggca gaagtaatga agatgaagcg acctcatatt gtgccgctct ctgagcaggt
    28861 cattatgcta tttaagcagc ttgaaccaat ctcaaaacat catcccctag tctttatcgg
    28921 tagaaatgat ccccgcaaac caattagtaa ggaaagcatt aaccaagtaa ttgaattact
    28981 tggatataag ggaagactca cagggcacgg tttcagacac accatgagta caattctgca
    29041 tgaacaaggc tttaattccg cctggattga aatgcagtta gctcatgtgg ataaaaattc
    29101 catcaggggt acctataatc atgccctgta tctcgatggc cgccgtgaaa tgatgcaatg
    29161 gtacgctgat tacattgatt cactctccag ccgggaaagt taagtcagaa atacttttcc
    29221 ctgatgtttt cctcaattaa gtattttatc ctctggccta caaggctgga ggatttttgt
    29281 tattatatta tttttttgcg tcttcaaact tattacaaaa acataattaa aaacaaattg
    29341 acatttttaa attggcaatt acaaaataca gccttgcagt tatttcgata cctttagcaa
    29401 aggaatagtt gattcagata taatttagca tttcaatcat ctgacatcat tataaaaaat
    29461 gagattcaca taaagataaa attattgttc tcaaggaaag catgaagggg cttcgtaagc
    29521 aatattcatt cggtgccaag acaggacttg atacacagaa tctcaacttt tatgctctca
    29581 gactatatca ccacatgttt tacaggctca gaaaatgaac gatagattat gctttgaagt
    29641 gcacgataac aagggttatt tcgtatttcc ggatacctgg tttggcccat tgctggggga
    29701 gtttgaagaa gtgcttgatg catacgacgc tgatgaaatc agcgagacga gttacatcaa
    29761 caaactccga cgccttgctc aacgggagcc ggactttatt gatatacacg ctcatctggc
    29821 atatgcattt ctggaacaga atgccccgcg taaggcactg aacgccgctc ttaaaggact
    29881 ggcggcaggt aatcgtatca tcccggaaag tttctgtggt gaaataatct ggatgcatcc
    29941 cgaaaaccgg ccttatctgc gcgcgcttta tgcagcaatt ctcgcaaatg tgcacttaca
    30001 gcgccatcag gatgcggtta tgctcactga taaaatactg gcatacaatc ctgaagataa
    30061 tcagggggct cgctggttgt tgggttcaga gctgttacgc accggcgatc atgagcgagc
    30121 cttcagtgta ctgaaagagc atgctgatga attttcacct tactggtatg aactcggtct
    30181 cctgcatttt ctgaacgggg aacatgttaa ggccgccaca gcttttcgcc atggctttgc
    30241 cacaaacact tacatcgcag aaatgctgtg cggaaatctg catccatttc cacttgctgt
    30301 gtggcatgac ttctccggct ctctggatac ggcagaagat tactatgcaa catactcacc
    30361 attatgggga caatatccgg aagcattgct gtttgtaaac tggctctata accattcctc
    30421 cgtgctgcat gaacgatcag aaatcattaa atgcgccgaa atgctgattc aggaagatga
    30481 tttcgaaata tgcgaaagta ttttaagaca gcaggagcac ctgtggaaaa gaattgacaa
    30541 gacactttct gaggaaattg tacagaaatg cagaaatatg aacggtgaat atatctggcc
    30601 ctggatattg cccttttccg cagcagggat gaaacattca agcatccagt atcagtagat
    30661 attgcattag tgtaacttta aaacaagtaa taatttatta tccaataata acactgtgtt
    30721 ccttccccag gtgattaccc tggggttatt ttctgaaagt ttcctttgca aaaggatagc
    30781 gttgtaaatt actttaacga attactccat gaactcatta aagttctttc gtaatacttt
    30841 atcgtcaaac acgttatagc tggcatcaaa aaccacagat ttaccctgcc agcgggtaat
    30901 cccctcctct ggcgttttcg gttgaactgg tatccccgtc caacacaggc agccattata
    30961 aacagtaaaa aattgtattc gctgttatga aatcaatagt cctgacgtac tgttgaatgt
    31021 gcttgtgttc tgaacctcgt acaaccagta catttgatat cgaattataa acaaaacatt
    31081 gcatccaact tttttaatat ggacaatagt tgtccatacg gtggttttat attcgataaa
    31141 tcaatggatt tcattgagtc acaggactgg taatggatga aaatgcttta aggtttgcct
    31201 catactggcg caactcgctt gcagatgctg agtcaggaaa gggctgtttt gaacggaaag
    31261 acgcccaaaa tttcactcac tggcatggga tagcggcggg acgtcttgac gaagcgattg
    31321 tcaataaatt ttttaaggga gaaaaagacg atgtcgaaac tgtcaatgtc atcttacgcc
    31381 caaaagttta tttccggtta ctgcagcatg gtaaggaccg ttccgcaggc acgcctgata
    31441 ttgttacacc gatagtgacg ccagccttgc taagccgtga gggtttttta tatccgacgc
    31501 cagcgacctc cattcccaga gacctgcttg aacctttgcc aaaaggagca ttttcgattg
    31561 gtgagattgg gcagtatgac aaatacaaga cgacccatac tgatcctacc cacgtaatgt
    31621 ggacacggcc ctaagcgaga ttatggtttt caaattgttc cgggctgaga ccgccacagg
    31681 cactgtgacg acgccagcga ttgtaatcac actcgatata attaaacacc gttgcccgca
    31741 ttatttcccg gctgctaaag cgttccccgt ggatacattc caccttcagc gaatgaaaga
    31801 agctttccac acaggcattg tcataacagt agcctttcgc actcatactg ccacgcaggt
    31861 tgtgtcgctt cagcattgcc tgataatccc ctgaacagta ttgaccacca cggtccgtat
    31921 gaacaatgac gctttccggg cgtcttctcc gccacaacgc catttgcagt gcatcacagg
    31981 ccagttgtgc tgtcattcgc ggtgacatcg accagccaat gacggcgcgt gaccacaggt
    32041 cgatgactac tgcgagatac aaccagccct catcggtacg caagtacgtg atgtcacccg
    32101 cccacttctg gtttcgggcc actggcgctg aagtcctgct ccagcagatt ctccaatacg
    32161 ggcaggccat gtgcacggta gctgaccggg ctgaacttcc ggctggcttt cgcccgcagc
    32221 ccctgacgac gcaggctggc ggcaatggtt ttaatattga actccggcag ttcgtcagca
    32281 aggcggggag caccgtatcg ctgctttgcc tcaaagaatg ccttatggac agcggcatcg
    32341 caggtgagcc gaaactgttg gcgcaggctc atctggtgac gacgcctgag ccagacatac
    32401 cagccgctgc gggcaacccg aagtacacga cacatcgctt tgatgctgaa ctctgcccga
    32461 tgattttcga taaagacata cttcatttca ggcgcttcgc gaagtatgtc gcggcctttt
    32521 ggaggatgga cagttcctca gcctgctccg ccagttgtcg tttaaggcgg acattttcag
    32581 cggccagttc gctttcgcgc tctgacgaac tcatttgttg ctgctgttta ctgcgccagg
    32641 cataaagctg agattcatac aggctgagtt cacgggctgc ggcggccaca ccgatgcgtt
    32701 cagcgagttt cagggcttcg ttacgaaatt caggcgtatg ttgtttacgg ggcttcttgc
    32761 tgattgatac tggttttgtc atgagtcacc tctggttgag agtttactca cttagtcctg
    32821 tgtccactat tggtgggtaa gatcaccctc cattaaaact aaaacgggcg ctttctgata
    32881 tatcatctgt aagcgcagta ataccattag tagcacctct cggcggcgca ttgattattg
    32941 aatattataa ctggaatgtt atatttcttg ttatgatgct gttcagtatt ataataatgt
    33001 tattatcacc aaagataact catgcaaatg agaaagtaat atgtcaaaac agtgcatcct
    33061 ttgtgaccaa agagtttatc tgcggcacta tacttgtctc attaatactc agttctctat
    33121 tttgctacat atcaatatca ccatcaattt tcatgcatac ttatggcctg agtactttta
    33181 gttactcaat tattttctcc ttctcagttt tatttttcat aatcggaaat caattatcaa
    33241 agattgaaaa attatccaac ctatggtttt tattacccaa tagccaattt tatcgcatta
    33301 caaataaatg gttcaaaaac aggatttgcc gcatctatta caggttttac tcaaacaata
    33361 agcgctggta caatcagttt cctgtcagta accgtaagcg acaacggtgt tggtcccgga
    33421 actactttag gtcttggttc actaataact tcattactgt caatagccgt aatcgccatt
    33481 gcaggaaata ataaaaagta agtgtactct gactgcgtat gtatctac
//
LOCUS       DAJTRB010000039        33447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTRB010000039 DAJTRB010000000
VERSION     DAJTRB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.039"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO330_001956"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO330_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5882835.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO330_001957"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO330_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5882836.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO330_001958"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO330_001958"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5882837.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO330_001959"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO330_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5882838.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO330_001960"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO330_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5882839.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO330_001961"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO330_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5882840.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO330_001962"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO330_001962"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5882841.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO330_001963"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO330_001963"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5882842.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO330_001964"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO330_001964"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5882843.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO330_001965"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO330_001965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5882844.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO330_001966"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO330_001966"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5882845.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO330_001967"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO330_001967"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5882846.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO330_001968"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO330_001968"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5882847.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO330_001969"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO330_001969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5882848.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO330_001970"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO330_001970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5882849.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO330_001971"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO330_001971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5882850.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO330_001972"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO330_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5882851.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO330_001973"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO330_001973"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5882852.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19506..20327
                     /locus_tag="OO330_001974"
     CDS             19506..20327
                     /locus_tag="OO330_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5882853.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20314..22422
                     /locus_tag="OO330_001975"
     CDS             20314..22422
                     /locus_tag="OO330_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5882854.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22419..24086
                     /locus_tag="OO330_001976"
     CDS             22419..24086
                     /locus_tag="OO330_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5882855.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24089..25615
                     /locus_tag="OO330_001977"
     CDS             24089..25615
                     /locus_tag="OO330_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5882856.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25616..27232
                     /locus_tag="OO330_001978"
     CDS             25616..27232
                     /locus_tag="OO330_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5882857.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27288..27440)
                     /locus_tag="OO330_001979"
                     /pseudo
     CDS             complement(27288..27440)
                     /locus_tag="OO330_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27463..27840)
                     /locus_tag="OO330_001980"
     CDS             complement(27463..27840)
                     /locus_tag="OO330_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882858.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28250..28621)
                     /locus_tag="OO330_001981"
     CDS             complement(28250..28621)
                     /locus_tag="OO330_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882859.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28682..29179)
                     /locus_tag="OO330_001982"
     CDS             complement(28682..29179)
                     /locus_tag="OO330_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882860.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29255..30043)
                     /gene="aadA1"
                     /locus_tag="OO330_001983"
     CDS             complement(29255..30043)
                     /gene="aadA1"
                     /locus_tag="OO330_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5882861.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(30101..30625)
                     /gene="sat2"
                     /locus_tag="OO330_001984"
     CDS             complement(30101..30625)
                     /gene="sat2"
                     /locus_tag="OO330_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5882862.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(30720..31193)
                     /locus_tag="OO330_001985"
     CDS             complement(30720..31193)
                     /locus_tag="OO330_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5882863.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            31525..32061
                     /locus_tag="OO330_001986"
     CDS             31525..32061
                     /locus_tag="OO330_001986"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5882864.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            32176..32502
                     /locus_tag="OO330_001987"
     CDS             32176..32502
                     /locus_tag="OO330_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5882865.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(32690..32929)
                     /locus_tag="OO330_001988"
     CDS             complement(32690..32929)
                     /locus_tag="OO330_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5882866.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         8674 a         7804 c         8452 g         8517 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cataagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaactatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acactatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgta tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc ttgtgggcgg
    19381 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
    19441 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
    19501 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
    19561 aggggcgtgg ccaagggcat ggtaaagact atattccatg gctaacagta caagaagttc
    19621 cttcttcagg tcgttcccac cgtatttatt ctcataagac gggacgagtc catcatttgc
    19681 tatctgactt agagcttgct gtttttctca gtcttgagtg ggagagcagc gtgctagata
    19741 tacgcgagca gttcccctta ttacctagtg ataccaggca gattgcaata gatagtggta
    19801 ttaagcatcc tgttattcgt ggtgtagatc aggttatgtc tactgatttt ttagtggact
    19861 gcaaagatgg tccttttgag cagtttgcta ttcaagtcaa acctgcagca gccttacaag
    19921 acgagcgtac cttagaaaaa ctagaactag agcgtcgcta ttggcagcaa aagcaaattc
    19981 cttggttcat ttttactgat aaagaaataa atcccgtagt aaaagaaaat attgaatggc
    20041 tttattcagt gaaaacagaa gaagtttctg cggagctttt agcacaacta tccccattgg
    20101 cccatatcct gcaagaaaaa ggagatgaaa acattatcaa tgtctgtaag caggttgata
    20161 ttgcttatga tttggagtta ggcaaaacat tgagtgagat acgagcctta accgcaaatg
    20221 gttttattaa gttcaatatt tataagtctt tcagggcaaa taagtgtgca gatctctgta
    20281 ttagccaagt agtgaatatg gaggagttgc gctatgtggc aaattaatga ggttgtgcta
    20341 tttgataatg atccgtatcg cattttggct atagaggatg gccaagttgt ctggatgcaa
    20401 ataagcgctg ataaaggagt tccacaagct agggctgagt tgttgctaat gcagtattta
    20461 gatgaaggcc gcttagttag aactgatgac ccttatgtac atcttgattt agaagagccg
    20521 tctgtagatt ctgtcagctt ccagaagcgc gaggaggatt atcgaaaaat tcttcctatt
    20581 attaatagta aggatcgttt cgaccctaaa gtcagaagcg aactcgttga gcatgtggtc
    20641 caagaacata aggttactaa ggctacagtt tataagttgt tacgccgtta ctggcagcgt
    20701 ggtcaaacgc ctaatgcatt aattcctgac tacaaaaaca gcggtgcacc aggggaaaga
    20761 cgttcagcga caggaacagc aaagattggc cgagccagag aatatggtaa gggtgaagga
    20821 accaaggtaa cgcccgagat tgaacgcctt tttaggttga ccatagaaaa gcacctgtta
    20881 aatcaaaaag gtacaaagac caccgttgcc tatagacgat ttgtggactt gtttgctcag
    20941 tattttcctc gcattcccca agaggattac ccaacactac gtcagtttcg ttatttttat
    21001 gatcgagaat accctaaagc tcagcgctta aagtctagag ttaaagcagg ggtatataaa
    21061 aaagacgtac gacccttaag tagtacagcc acttctcagg cgttaggccc tgggagtcgt
    21121 tatgagattg atgccacgat tgctgatatt tatttagtgg atcatcatga tcgccaaaaa
    21181 atcataggaa gaccaacgct ttacattgtg attgatgtgt ttagtcggat gatcacgggc
    21241 ttttatatcg gctttgaaaa tccgtcttat gtggtggcga tgcaggcttt tgtaaatgct
    21301 tgctctgaca aaacggccat ttgtgcccag catgatattg agattagtag ctcagactgg
    21361 ccgtgtgtag gtttgccaga tgtgttgcta gcggaccgtg gcgaattaat gagtcatcag
    21421 gtcgaagcct tagtttctag ttttaatgtg cgagtggaaa gtgctccacc tagacgtggc
    21481 gatgctaaag gcatagtgga aagcactttt agaacactac aagccgagtt taagtccttt
    21541 gcacctggca ttgtagaggg cagtcggatc aaaagccatg gtgaaacaga ctataggtta
    21601 gatgcatctc tgtcggtatt tgagttcaca caaattattt tgcgtacgat cttattcaga
    21661 aataaccatc tggtgatgga taaatacgat cgagatgctg attttcctac agatttaccg
    21721 tctattcctg tccagctatg gcaatggggt atgcagcatc gtacaggtag tttaagggct
    21781 gtggagcaag agcagttgcg agtagcgtta ctgcctcgcc gaaaggtctc tatttcttca
    21841 tttggcgtta atttgtgggg tttgtattac tcggggtcag agattctgcg tgagggttgg
    21901 ttgcagcgga gcactgatat agctagacct caacatttag aagcggctta tgacccagtg
    21961 ctggttgata cgatttattt gtttccgcaa gttggcagcc gtgtattttg gcgctgtaat
    22021 ctgacggaac gtagtcggca gtttaaaggt ctctcatttt gggaggtttg ggatatacaa
    22081 gcacaagaaa aacacaataa agccaatgcg aagcaggatg agttaactaa acgcagggag
    22141 cttgaggcgt ttattcagca aaccattcag aaagcgaata agttaacgcc cagtactact
    22201 gagcccaaat caacacgcat taagcagatt aaaactaata aaaaagaagc cgtgacctcg
    22261 gagcgtaaaa aacgtgcgga gcatttgaag ccaagctctt caggtgatga ggctaaagtt
    22321 attcctttca acgcagtgga agcggatgat caagaagatt acagcctacc cacatacgtg
    22381 cctgaattat ttcaggatcc accagaaaag gatgagtcat gagtgctacc cggattcaag
    22441 cagtttatcg tgatacgggg gtagaggctt atcgtgataa tccttttatc gaggccttac
    22501 caccattaca agagtcagtg aatagtgctg catcactgaa atcctcttta cagcttactt
    22561 cctctgactt gcaaaagtcc cgtgttatca gagctcatac catttgtcgt attccagatg
    22621 actattttca gccattaggt acgcatttgc tactaagtga gcgtatttcg gtcatgattc
    22681 gaggtggcta cgtaggcaga aatcctaaaa caggagattt acaaaagcat ttacaaaatg
    22741 gttatgagcg tgttcaaacg ggagagttgg agacatttcg ctttgaggag gcacgatcta
    22801 cggcacaaag cttattgtta attggttgtt ctggtagtgg gaagacgacc tctcttcatc
    22861 gtattctagc cacgtatcct caggtgattt accatcgtga actcaatgta gagcaggtgg
    22921 tgtatttgaa aatagactgc tcgcataatg gttcgctaaa agaaatctgc ttgaattttt
    22981 tcagagcgtt ggatcgagcc ttgggctcga actatgagcg tcgttatggc ttaaaacgtc
    23041 atggtataga aaccatgttg gctttgatgt cgcaaatagc caatgcacat gctttagggt
    23101 tgttggttat tgatgaaatt cagcatttaa gccgctctcg ttcgggtgga tctcaagaga
    23161 tgctgaactt ttttgtgacg atggtgaata ttattggcgt accagtgatg ttgattggta
    23221 cccctaaagc acgagagatt tttgaggctg atttgcggtc tgcacgtaga ggggcagggt
    23281 ttggagctat attctgggat cctatacaac aaacgcaacg tggaaagccc aatcaagagt
    23341 ggatcgcttt tacggataat ctttggcaat tacagctttt acaacgcaaa gatgcgctgt
    23401 tatcggatga ggtccgtgat gtgtggtatg agctaagcca aggagtgatg gacattgtag
    23461 taaaactttt tgtactcgct cagctccgtg cgctagcttt aggcaatgag cgtattaccg
    23521 ctggtttatt gcggcaagtg tatcaagatg agttaaagcc tgtgcacccc atgctagagg
    23581 cattacgctc gggtatccca gaacgcattg ctcgttattc tgatctagtc gttcccgaga
    23641 ttgataaacg gttaatccaa cttcagctag atatcgcagc gatacaagaa caaacaccag
    23701 aagaaaaagc ccttcaagag ttagataccg aagatcagcg tcatttatat ctgatgctga
    23761 aagaggatta cgattcaagc ctgttaattc ccactattaa aaaagcgttt agccagaatc
    23821 caacgatgac aagacaaaag ttactgcctc ttgttttgca gtggttgatg gaaggcgaaa
    23881 cggtagtgtc agaactagaa aagccctcca agagtaaaaa ggtttcggct ataaaggtag
    23941 tcaagcccag cgactgggat agcttgcctg atacggattt acgttatatc tattcacaac
    24001 gccaacctga aaaaaccatg catgaacggt taaaagggaa aggggtaata gtggatatgg
    24061 cgagcttatt taaacaagca ggttagccat gagaaacttt cctgttccgt actcgaatga
    24121 gctgatttat agcactattg cacgggcagg cgtttatcaa gggattgtta gtcctaagca
    24181 gctgttggat gaggtgtatg gcaaccgcaa ggtggtcgct accttaggtc tgccctcgca
    24241 tttaggtgtg atagcaagac atctacatca aacaggacgt tacgctgttc agcagcttat
    24301 ttatgagcat accttattcc ctttatatgc tccgtttgta ggcaaggagc gccgagacga
    24361 agctattcgg ttaatggagt accaagcgca aggtgcggtg catttaatgc taggagtcgc
    24421 tgcttctaga gttaagagcg ataaccgctt tagatactgc cctgattgcg ttgctcttca
    24481 gctaaatagg tatggggaag ccttttggca acgagattgg tatttgcccg ctttgccata
    24541 ttgtccaaaa cacggtgctt tagtcttctt tgatagagct gtagatgatc accgacatca
    24601 attttgggct ttgggtcata ctgagctgct ttcagactac cccaaagact ccctatctca
    24661 attaacagca ctagctgctt atatagcccc tctgttagat gctccacgag cgcaagagct
    24721 ttccccaagc cttgagcagt ggacgctgtt ttatcagcgc ttagcgcagg atctagggct
    24781 aaccaaaagc aagcacattc gtcatgactt ggtggcggag agagtgaggc agacttttag
    24841 tgatgaggca ctagagaaac tggatttaaa gttggcagag aacaaggaca cgtgttggct
    24901 gaaaagtata ttccgtaagc atagaaaagc ctttagttat ttacagcata gtattgtgtg
    24961 gcaagcctta ttgccaaaac taacggttat agaagcgcta cagcaggcaa gtgctcttac
    25021 tgagcactct ataacgacaa gacctgttag ccagtctgtg caacctaact ctgaagattt
    25081 atctgttaag cataaagact ggcagcaact agtgcataaa taccaaggaa ttaaggcggc
    25141 aagacagtct ttagagggtg gggtgctata cgcttggctt taccgacatg acagggattg
    25201 gctagttcac tggaatcaac agcatcaaca agagcgtctg gcacccgccc ctagagttga
    25261 ttggaaccaa agagatcgaa ttgctgtacg acaactatta agaatcataa agcgtctaga
    25321 tagtagcctt gatcacccaa gagcgacatc gagctggctg ttaaagcaaa ctcctaacgg
    25381 aacctctctt gcaaaaaatc tacagaaact gcctttggta gcgctttgct taaagcgtta
    25441 ctcagagagt gtggaagatt atcaaattag acggattagc caagctttta ttaagcttaa
    25501 acaggaagat gttgagctta ggcgctggcg attattaaga agtgcaacgt tatctaaaga
    25561 gcggataact gaggaagcac aaagattctt ggaaatggtt tatggggaag agtgagtggt
    25621 taggctagct acatttaatg acaatgtgca ggttgtacat attggtcatt tattccgtaa
    25681 ctcgggtcat aaggagtggc gtatttttgt ttggtttaat ccaatgcaag aacggaaatg
    25741 gactcgattt actcatttgc ctttattaag tcgagctaag gtggttaaca gtacaacaaa
    25801 gcaaataaat aaggcggatc gtgtgattga gtttgaagca tcggatcttc aacgagccaa
    25861 aataatcgat tttcctaatc tctcgtcctt tgcttccgta cgcaacaagg atggagcgca
    25921 gagttcattt atttacgaag ctgaaacacc atatagcaag actcgttatc acatcccaca
    25981 gttagagcta gctcggtcat tatttttaat taactcctat ttctgtcgaa gctgtttgag
    26041 cagtaccgct ttacagcaag agttcgacgt tcagtatgag gttgagcgag atcatttaga
    26101 gataaggatc ttacccagtt catcgtttcc taaaggggcg ttagagcagt cggccgtagt
    26161 gcagcttttg gtttggttgt tttcggatca agatgttatg gattcgtatg aaagtatttt
    26221 taggcactat caacaaaata gagaaattaa gaacggcgtt gaaagctggt gctttagctt
    26281 tgaccctccg cccatgcagg gttggaaatt acatgtaaaa ggacgttctt ctaacgagga
    26341 taaggattat ttagttgagg aaatagtagg tttagaaatc aacgctatgc ttcctagcac
    26401 aacagctatt agccatgcct cttttcagga aaaggaggca ggtgatggta gtacgcagca
    26461 catagcggtt tcaacagagt cagttgttga tgatgagcat ctacagttgg acgatgagga
    26521 aacagccaat atagacacag acacacgagt catagaggct gagccgacat ggataagttt
    26581 tagtagacct agtcgaattg aaaaatctcg cagggcaaga aaaagtagcc aaactatttt
    26641 agaaaaagaa gaagcaacaa caagtgaaaa tagtaatttg gttagtactg atgagccaca
    26701 cttaggtggt gtcctagcag cggcagatgt gggtgggaag caggatgcaa ccaattacaa
    26761 ctctattttt gctaatcgat ttgctgcttt tgatgagcta ctttcaattc taaaaactaa
    26821 atttgcatgt cgggtgcttt ttgaagaaac cttggttttg ccaaaagttg ggcgtagccg
    26881 attacatctg tgtaaagatg gctcaccaag agtgattaaa gccgttgggg tgcaacgtaa
    26941 tggcagtgaa tttgtattgc tagaggtgga tgcatcggat ggggtgaaaa tgctttctac
    27001 caaagtgttg agtggcgttg atagcgaaac atggcggaat gattttgaaa agatacggcg
    27061 tggagtggtg aagagctcat tgaattggcc aaatagtttg tttgatcaat tatatggaca
    27121 agacgggcat agaggggtga atcatccaaa ggggttgggg gagctgcaag tatcgagaga
    27181 ggatatggaa gggtgggctg agagagtggt tagagagcaa tttacgcatt aaaggaatga
    27241 ctgaaagagc ctgtaaaccc ttttgtgtaa gtgcttttgc cggtcagtta aaggtggcca
    27301 tttaaacggt caccaaattc gatcataaaa cggttcatgg ccggcttcca gttgcggatc
    27361 ggcatcgtcc atttcttggt cgccgcctgg atagccaggt acaccacctt catcgctgat
    27421 tcgtccgtag ggaacacctt gagttcatat taatggaatt ttctacaaat agcctccgtg
    27481 gttttgaggg gggattacag acgatccata gtagtaatcc aatgagttct tgagcgcggc
    27541 gacatgtttg gacgccttgg caaaaattag agcctgcttg aagtgcaggc gagcacgtgc
    27601 ttggacgatt gatccattcg cggtcaaaaa ctcaatcttg gatgacagcg tgtcaggaaa
    27661 tccaatatcg aagtcccgcc ccgtgtcaaa gtagggcatt tcatgatcaa aggacgaagc
    27721 ttcccaggcc acttccctaa tattccagcc gctagaaaca agatttcgta cgacttcatc
    27781 aacttttttg ctttccaact tgtcacaaag tgcataggca agagctttgg cggcagacac
    27841 gtgcgtttcc tcttaaacat aggtcgaacg gatacccgag gcaaagcgga gcattagtcc
    27901 caaagcatct ccggtagatc cggaggctcc atactgaagg catggcatct tgggcattga
    27961 tatttgaaac ttctctctag atagcaggga tttcttggcc ttgcctcatg ctgatagctt
    28021 gtaaatatca cacgatcctt agccccacaa agtgagcata ctccgtagct ttcatttata
    28081 gaaatcgtcg ttagattgtc gcagcatgtg cacgttccga ctatttgctc aggaagcatc
    28141 gccatacctt ggcctaggta aagaagatca gactcgtagc cacatcggtg gcaactgagc
    28201 ttgtacgcaa tgcccatctg gttgtctcac ctgaatcgta acgtcagagc tattcatcca
    28261 tccattcggc atcaatgtat gccactaagc cgaccagctg accgtttgga ctgaagtatg
    28321 ttgcctgcac cccacttgac atctcatcaa taccaccgca ctgcgctagg cgccggtccc
    28381 acttgcccac agaagcgtca ggatcggctg agttccccaa gacccttgtg agccatgcct
    28441 cctttaactc aacctctcga atgattgcgt cggccagctc ctccgcgtta tctagttcga
    28501 catagtctgg aattcggacg tacgttctga acacagctgg atacttactt gggcgattgt
    28561 catacatgac atcaacaatg tacccgtttg tgtaaccgtc tcttggaggt tcgtatgaca
    28621 ctagtggttc ccctcagctt gcgactagat gttgaggcct aacattttat tagagagcag
    28681 gctagttgct tagatacatg atcttcaggc cgttatctgt cagggcaagc gaaaattggc
    28741 catttatgac gaccaatgcc ccgcagaagc tcccatcttt gccgccatag acgccgcgcc
    28801 ccccttttgg ggtgtagaac atccttttgc cagatgtgga aaagaagttc gttgtcccat
    28861 tgttggcaat gacgtagtag ccggcgaaag tgcgagaccc atttgcgcta tatataagcc
    28921 tacgatttcc gttgcgacta ttgtcgtaat tggatgaact attatcgtag ttgctctcag
    28981 agttgtcgta atttgatgga ctattgtcgt aattgcttat ggagttgtcg tagttgcttg
    29041 gagaaatgtc gtagttggat ggggagtagt catagggaag acgagcttca tccactaaaa
    29101 caattggcag gtcagcaagt gcctgccccg atgccatcgc aagtacgagg cttagaacca
    29161 ccttcaacag atcgcgcata gtcttcccca gctctctaac gcttgagtta agccgcgccg
    29221 cgaagcggcg tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct
    29281 cgcctttcac gtagtgaaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
    29341 cttgtccaag ataagcctgc ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
    29401 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
    29461 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
    29521 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
    29581 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
    29641 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
    29701 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
    29761 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
    29821 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
    29881 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
    29941 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
    30001 ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt aactcctgaa
    30061 ttaagccgcg ccgcgaagcg gtgtcggctt gaatgaattg ttaggcgtca tcctgtgctc
    30121 ccgagaacca gtaccagtac atcgctgttt cgttcgagac ttgaggtcta gttttatacg
    30181 tgaacaggtc aatgccgccg agagtaaagc cacattttgc gtacaaattg caggcaggta
    30241 cattgttcgt ttgtgtctct aatcgtatgc caaggagctg tctgcttagt gcccactttt
    30301 tcgcaaattc gatgagactg tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa
    30361 tgtgttcgat agaggctaga tcgttccatg ttgagttgag ttcaatcttc ccgacaagct
    30421 cttggtcgat gaatgcgcca tagcaagcag agtcttcatc agagtcatca tccgagatgt
    30481 aatccttccg gtaggggctc acacttctgg tagatagttc aaagccttgg tcggataggt
    30541 gcacatcgaa cacttcacga acaatgaaat ggttctcagc atccaatgtt tccgccacct
    30601 gctcagggat caccgaaatc ttcatatgac gcctaacgcc tggcacagcg gatcgcaaac
    30661 ctggcgcggc ttttggtaca aaaggcgtga caggtttgcg aatccgttgc tgccacttgt
    30721 taaccctttt gccagatttg gtaactataa tttatgttag aggcgaagtc ttgggtaaaa
    30781 actggcctaa aattgctggg gatttcagga aagtaaacat caccttccgg ctcgatgtct
    30841 attgtagata tatgtagtgt atctacttga tcgatcaggc ttttgtatat ctccccacca
    30901 cctgaaacaa tgacatgatc cgttattttc tttaggttgg ttaaagcatc tttaattgat
    30961 ggaaagatca atacgttctc attgtcagat gtaaaacttg aacgtgttac gaccgcatac
    31021 tttcggttgg gtaatgctcc cattgattca aaagtcttgc gtccaaccaa cagccattgg
    31081 ttataggtaa tagctttaaa caggagctgt tcacctttgg cactccatgg aatatcaggg
    31141 ccattcccga taactccatt cttcgatata gctaccatta gtgatagttt cacaattctt
    31201 cctcagaggt taacatttta ttagtaagca tgctcgtttt gtcacccgct gatgcttacc
    31261 gttaattaat ataatcactt gtattaaata gactttcttt aaaaatacaa gacactctgt
    31321 tattacaaat cgtgcatgcc gtctatcctg cttgcacgat gcacattatc tcacccaaag
    31381 aactcataac attttaattt atttagcatt aaattaaaac tctaccggta aacaagcatc
    31441 tctaggcgta aaaaacaggc acttttttaa atcccagcta gaataggctg tataggcaga
    31501 cagttgcaag acaaggagcg ttttatgtct aacagtccat ttttaaattc tatacgcacg
    31561 gatatgcgac aaaaaggtta tgcgctgaaa actgaaaaaa cttacctgca ctggattaag
    31621 cgttttattc tgtttcacaa aaaacgtcat cctcagacca tgggcagtga agaggtcagg
    31681 ctgtttttat ccagcttagc aaacagcaga catgtagcca taaacacgca gaaaatcgct
    31741 ttaaatgccc tagctttttt gtacaacagg tttttacaac agccgttggg cgatattgat
    31801 tatatccctg caagcaagcc tagacggcta ccctctgtta tctctgcaaa tgaagtgcaa
    31861 cgcattttgc aggttatgga tactcgcaac caagttattt ttacgctgct gtatggtgca
    31921 ggtttgcgca ttaatgaatg cttgcgtttg cgggttaaag attttgattt tgataatggc
    31981 tgcatcactg tgcatgacgg taagggtggg aaaagcagaa acagcctact gcccacgcgc
    32041 ctaatcccag caataaaata actcattgag caagcgcggc ttattcagca agacgacaac
    32101 ttacaaggcg tagggccatc gctgcctttt gctttagatc acaaataccc ttctgcttat
    32161 cgacaagcgg cgtggatgtt tgtctttccc tccagcacgc tctgcaacca cccgtataac
    32221 ggcaaattat gccgccatca tctgcatgac tccgttgcgc gaaaggcatt gaaggcagcc
    32281 gtacaaaaag caggcatcgt tagcaagcgt gtcacttgtc atacatttcg tcactcgttt
    32341 gctacgcatc tattacaagc ggggcgtgat attcgcactg tgcaagaact cttagggcat
    32401 aacgatgtta agaccacgca aatctatacg catgtgttgg gtcagcattt tgccggcacc
    32461 accagtcctg cggatggact gatgctactt atcaatcagt aaaagtagat aaatatcttt
    32521 atgtagccct gagtagcccc tgatactttt tatggtgaaa aagataaaca atcatagagg
    32581 ggataaatat gagccagttc taccattgtt cagataagtt taagcaagat actgtcaatc
    32641 acagtgacca ttcatggtca ttacacttcc tccagttgcc agacaatgcc taacataaaa
    32701 acgcaaaagc ccaaaagtca gagcttaggt gttctaggcg ttttctaagc gctgaagaaa
    32761 tacgtcttac cgtagattgc gtgcgtttct gaagcacatt ttccttatac atggctttat
    32821 cccaggcagc ttcatcaagc tcttggagta acaagccagt aataatgcga cactctcggg
    32881 tttgtagcga gccgctggtg agatcggagt tatagcggaa aggggtcatt tgtaattatt
    32941 tgtgggtgga ggatagttaa cttattattg tagttggaag aggctgcttt ccaaacatta
    33001 gcacctcgta atgcttgtta ctgtatatta atttatgctc ttagcgcatg ttaatgggct
    33061 attacaatgc ataatgtcca catcaagata actgtggctt aacacctgta caagcgaaaa
    33121 aaatcaatca cctgtccgga aatacgggat cactgtaaag tgttggtgag gcagtctagg
    33181 agggggattt aatttgggat actgatgaaa ggattatgat cagttggata taggatcgta
    33241 ctaagctgag cggcaggtta gctagattag ttaaccagat aagtgaaatc tagttccaaa
    33301 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
    33361 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
    33421 ctattttgtc cgcccacagg gctgccg
//
LOCUS       DAJTRB010000040        33154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTRB010000040 DAJTRB010000000
VERSION     DAJTRB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.040"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(2..94)
                     /locus_tag="OO330_001989"
     tRNA            complement(2..94)
                     /locus_tag="OO330_001989"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO330_001990"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO330_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5882867.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO330_001991"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO330_001991"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5882868.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO330_001992"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO330_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5882869.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO330_001993"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO330_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5882870.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO330_001994"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO330_001994"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5882871.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO330_001995"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO330_001995"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5882872.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO330_001996"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO330_001996"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO330_001997"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO330_001997"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5882873.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO330_001998"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO330_001998"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5882874.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO330_001999"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO330_001999"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5882875.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO330_002000"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO330_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO330_002001"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO330_002001"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5882876.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMAPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO330_002002"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO330_002002"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5882877.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO330_002003"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO330_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5882878.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO330_002004"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO330_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5882879.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO330_002005"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO330_002005"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5882880.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO330_002006"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO330_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5882881.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO330_002007"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO330_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5882882.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO330_002008"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO330_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5882883.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO330_002009"
     CDS             complement(20900..21019)
                     /locus_tag="OO330_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5882884.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO330_002010"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO330_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5882885.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO330_002011"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO330_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5882886.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25505)
                     /gene="hycC"
                     /locus_tag="OO330_002012"
     CDS             complement(23682..25505)
                     /gene="hycC"
                     /locus_tag="OO330_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5882887.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLF
                     KSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWV
                     IYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENA
                     TCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIA
                     CPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLR
                     KWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25542..26153)
                     /gene="hycB"
                     /locus_tag="OO330_002013"
     CDS             complement(25542..26153)
                     /gene="hycB"
                     /locus_tag="OO330_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5882888.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26278..26739)
                     /gene="hycA"
                     /locus_tag="OO330_002014"
     CDS             complement(26278..26739)
                     /gene="hycA"
                     /locus_tag="OO330_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5882889.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26951..27301
                     /gene="hypA"
                     /locus_tag="OO330_002015"
     CDS             26951..27301
                     /gene="hypA"
                     /locus_tag="OO330_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5882890.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27305..28177
                     /gene="hypB"
                     /locus_tag="OO330_002016"
     CDS             27305..28177
                     /gene="hypB"
                     /locus_tag="OO330_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5882891.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28168..28440
                     /gene="hypC"
                     /locus_tag="OO330_002017"
     CDS             28168..28440
                     /gene="hypC"
                     /locus_tag="OO330_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5882892.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28440..29561
                     /gene="hypD"
                     /locus_tag="OO330_002018"
     CDS             28440..29561
                     /gene="hypD"
                     /locus_tag="OO330_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5882893.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29558..30568
                     /gene="hypE"
                     /locus_tag="OO330_002019"
     CDS             29558..30568
                     /gene="hypE"
                     /locus_tag="OO330_002019"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5882894.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30642..32720
                     /gene="flhA"
                     /locus_tag="OO330_002020"
     CDS             30642..32720
                     /gene="flhA"
                     /locus_tag="OO330_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5882895.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32757..33110)
                     /locus_tag="OO330_002021"
     CDS             complement(32757..33110)
                     /locus_tag="OO330_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5882896.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7808 a         9249 c         8629 g         7468 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 agatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgacatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggctc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttataccca gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagccccag caggataatg
    24661 ccgatatttt ccagggtgtg gtaagccagc aggcgctgga tgttgtgttc catcaacgca
    24721 tacagaccac cgacgaacgc ggtgatcatg ccgagcacca gcagcgcgat cccccaccac
    24781 agcggtgcat taccgcccag cagtgacagg gttaacatgc ccagcaggcc aattttcatg
    24841 actaccgtag aaaacaacgc ggcagctggc gcagaggcgt tagcatgtgc ctgcggcacc
    24901 cagccgtgca gcggaataat cccagccagc aggccaaagc caatcacacc gagcagccag
    24961 atatcggaac cgagcggcag ctgttgcata cgcatatcca gcaggcgcag atccagcgtg
    25021 ccataacgct gccacagcag ccagcaagca atcaccagca gcagagtgcc aagacgcccc
    25081 agcgcaaacc acagtttgcc ctctttgctg ttgctggtga ggaacaccgc acacaaggcc
    25141 atgacttcgg ccattaccac gaacatgccg aggttgctgg cgatgacggc gcagacggcg
    25201 gcagccatca acatattgat ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag
    25261 tcaatgttat agaggctgac aaacagaccg caaaggccga gggtaatcag ccaaatcgcg
    25321 ttaagcgggg agatttgcac atcgtagctt accagcgaca gcgcaccgct cacgccaacc
    25381 gcgccagtca gtacagtgaa gcccgcggct gccgtataca gactaccaac cgcgccgcca
    25441 attccggcta tccagccact tagcgctttt tgaaaagaga aatgccagaa cagcggcggc
    25501 gacaaaccat gccacgccgc tattgatcag ggaaattgcg ctcatttagc ctctccactt
    25561 tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt taaaggtcag ctcacgctta
    25621 cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt agtcgggcac
    25681 atccgcacac aggccggacc ttgttcatca aagctacaaa ggtcacattt gacggcgatc
    25741 gcgcgaatac ctggcaccca gtcaagcaat gtgctgacac gcgccggagc aggcggtgcc
    25801 ggtggcgctt tcggggtatt ggcgtttgcc ggaatatcca gcggacggct gccggaaaat
    25861 tcaattgcgc caaacgggca ggcgatgccg cacagtttgc agcttacgca cagactttca
    25921 ttcaactgca cggccccatc aacgcgggtg atggcgttaa ccgggcagac caccgcgcag
    25981 ggtgcatctt cacagtggtg acagagctgc ggcgcagatt ctttttcatt gagcatcact
    26041 ctcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca ggcggcctca
    26101 caggtgtggc agccgataca gagcgtggag tcggcaatta caaaacgatt caccaggcat
    26161 tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt ttcgacactc
    26221 atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag cctcagctca
    26281 tgctgccggg ctttgtccct ttaccagttg gcttaaattc accggcacat tgttttcaat
    26341 ggcggtgtat aagctgtcgt aaacgccagc gattttttcg agatagtgtt ccagaacctg
    26401 ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca gttgcgcctg
    26461 cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg taatactcga tggtgtggct
    26521 gttaaagcca tccgccaggg tgacgtaaat acgaaaatgt tcaaaaagga cgaaacagag
    26581 ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata acgtgatccc
    26641 ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat gccgctgtgc
    26701 gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac tcgtttaaca
    26761 gagatgccag ctttaagcat tttttgtgcc aacttttaat ttattgttat taaagagatt
    26821 tttaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat catcgacatt
    26881 attcaccgca gggataatca acactggcac aattattgct tgtagctggc aatagttaat
    26941 gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga tcgaacagca
    27001 ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg gcgcattttc
    27061 ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg gcagcgtggc
    27121 ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg aaacatgcca
    27181 acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc atggtgacat
    27241 gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag accaggagtg
    27301 agcgatgtgt acaacatgcg gttgcggtga aggcaacctg tatatcgagg gtgatgaaca
    27361 taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc cgcagatgaa
    27421 aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag gcgacctgca
    27481 ttacggtcat ggcgaagcgg gcactcacac accgggcatg agccagcgtc ggatgctgga
    27541 agtcgaaatt gacgtgctgg acaaaaacaa ccgtctggct gaacgcaacc gcgcgcgctt
    27601 tgctgcccgc aagcaactgg tgctcaacct ggtttccagc cctggttccg gtaaaaccac
    27661 cctgctgacg gaaaccttaa tgcgcctgaa agacagcgtt ccgtgcgcgg ttattgaagg
    27721 cgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggtacac cagcgattca
    27781 ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg ccgcaccgcg
    27841 tctgccactg gacgataacg gtattctgtt tatcgaaaac gttggcaacc tcgtatgccc
    27901 ggccagcttc gatctcggtg aaaaacacaa agtagcggtg ctttccgtta ccgaaggtga
    27961 agacaaaccg ctgaaatatc cgcatatgtt tgccgccgcc tcgctgatgc tgctcaacaa
    28021 agttgacctg ttgccgtatc tcaactttga cgttgagaag tgcatcgcct gcgcccgcga
    28081 agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggcgaag ggatggacca
    28141 gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca gatccgcacc
    28201 attgacggca accaggcgaa agtcgacgtc tgcggcattc agcgcgatgt cgatttaacg
    28261 ttagtcggca gctgcgatga aaacggtcag ccgcgcgtgg gccagtgggt actggttcac
    28321 gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct cgacgcctta
    28381 caaaacatgt ttgacgttga gccggatgtc ggcgcgctgt tgtatggcga ggaaaaataa
    28441 tgcgttttgt tgatgaatat cgcgcgccgg aacaggtgat gcagttaatt gagcatctgc
    28501 gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt atggaagtgt
    28561 gtggcggtca tacccacgcc atttttaaat tcggcctcga ccagttactg cctgaaaacg
    28621 ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccgatgggt agaatcgaca
    28681 cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc gacgccatgc
    28741 gcgtaccagg gaaacaggga tcgctgttgc aggcaaaagc acgcggtgcc gatgtgcgca
    28801 tcgtttactc gccgatggat gcgttgaaac tggcgcagga gaatccaacc cgcaaagtgg
    28861 tgttcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact ctgcaacagg
    28921 cgaaagcgcg tgatgtgcag aatttttact tcttctgcca gcacattacg cttatcccga
    28981 cgctgcgcag tttgctggaa cagccggata acggtatcga cgcgttcctc gcgccgggcc
    29041 acgttagtat ggttatcggc actgatgcct ataattttat cgccagcgat tttcatcgtc
    29101 cgctggtggt cgctggtttc gaaccccttg atctactgca aggcgtggtc atgctggtgg
    29161 agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg gtaccggatg
    29221 ccggtaacct gctggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac ggcgacagcg
    29281 aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg gattatcaac
    29341 gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat gacccgcgcg
    29401 cgcgttgtgg tgaggtatta acgggcaaat gtaagccgca tcaatgcccg ctgtttggta
    29461 acacctgtaa tcctcaaacc gcatttggtg cactgatggt ctcctccgaa ggagcgtgcg
    29521 ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc aactcgccca
    29581 cggcagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg aagcctttgc
    29641 caacccgtgg ctggcagagc aggaagatca ggcacgtctt gatctggcgc agctggtagc
    29701 ggaaggcgac cgtctggcgt tctccaccga cagttacgtc attgacccgc tgttctttcc
    29761 tggcggtaat atcggcaagc tggcgatttg cggcaccgcg aatgacgttg cgggcagtgg
    29821 cgctattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc cgatggagac
    29881 actgaaagcc gtagtgacca gcatggcaga aaccgcccgc acggcaggca ttgccatcgt
    29941 tactggcgat actaaagtgg tgcagcgcgg cgcggcagat aaactgttta tcaacaccgc
    30001 tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa ccgcaggcga
    30061 tgtattgctg gttagcggta cactcggcga ccacggggcg actatcctta acctgcgtga
    30121 gcagctgggg ctggatggcg aactggtcag cgactgcgcg gtgctgacgc cgcttattca
    30181 gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg gtggtgtaaa
    30241 cgcggtggtt catgagttcg cggcagcctg cggttgtggt attgaaattt ctgaatcagc
    30301 gctgccggtt aaacctgccg tgcgcggcgt ttgcgaattg ctgggactgg acgccctgaa
    30361 ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc aacgcggcag agcaagtgct
    30421 ggcagcgtta cattcccatc cactggggaa agacgcggcg ctgattggtg aagtggtgga
    30481 acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg atttaccaca
    30541 cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag ttagtcaatg
    30601 accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat acaccgatga
    30661 gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag cagcccgatc
    30721 tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg
    30781 cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgcg tcgcgtgaaa
    30841 aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg gtacgcagca
    30901 ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa acctggccgc
    30961 agctggccgc aggtgggcta taccccaaat ttggtcacta ttgcctgatg ccactggcgg
    31021 cgggagggca tatttttggt ggctgtgaat ttattcgtta tgacgagcgt ccctggagcg
    31081 aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc accgaacaaa
    31141 tccagagccg cgtcgtcaac aatgtcgact atgagttgtt atgccgggag cgcgataact
    31201 tccgcatcct ggttgccatt accaacgcgg tgctttcccg cctggatatg gacgaactgg
    31261 tcagcgaagt cgccaaagaa atccattact atttcgacat tgacgatatc agcattgtct
    31321 tacgcagcca ccgtaaaaac aaactcaaca tctactccac tcactatctt gataaacaac
    31381 atcccgccca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa cgcgtgttca
    31441 aaagtaaaga gatgctgctg atcaatctct acgagcggga cgatttagcc ccctatgaac
    31501 gcatgttgtt cgacacctgg ggcaaccaga ttcaaacctt gtgcctgtta ccgctgatgt
    31561 ctggcgacac catgctgggc gtgctgaaac tggcgcaatg cgaagagaaa gtgtttacca
    31621 ctaccaatct gaatttgctg cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg
    31681 ccctcgccta tcaggaaatc catcgtctga aagaacggct ggttgatgaa aacctcgccc
    31741 tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc cgcagcgaag
    31801 ccatgtacag cgtgcttaaa caagttgaaa tggtggcgca aagtgacagt accgtgctga
    31861 tcctcggtga aactggctcg ggtaaagagc tgattgcccg tgcgatccat aatctcagtg
    31921 ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg
    31981 aaagcgatct gtttggtcat gagcgtgggg cttttaccgg tgccagcgcc cagcgcattg
    32041 gtcgttttga actggcggat aaaagctctc tgttcctcga cgaagtgggc gatatgccac
    32101 tggagttaca gccgaagttg ctgcgtgtat tgcaggaaca ggagtttgaa cgtctcggca
    32161 gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc gatctgaaaa
    32221 aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgcctgaac gtattcccga
    32281 ttcacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg aaagccttta
    32341 ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc gagacgctgc
    32401 gcaccttgag caacatggag tggccgggta acgtacgcga actggaaaac gtcattgagc
    32461 gcgcagtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat attgctttac
    32521 cggagcctga aacgccgcct gccgcaacgg ttgtcgccca ggagggcgaa gatgaatatc
    32581 agttgattgt gcgcgtgctg aaagaaacca acagcgtggt tgccgggcct aaaggcgctg
    32641 cgcaacgtct ggggctgaaa cgcacgaccc tgctgtcacg gatgaagcgg ctgggaattg
    32701 ataaatcggc attgatttaa ctgcaaattg ccggacagat ctgcctgtcc ggcatactat
    32761 tcacgaggtt ttttcggacg atatttttcc ggcagttctg gcaccggacg cttgtcatcg
    32821 atgagatgac gcacggttaa gatcggatga cgccacagca ttctcggccc ggcccaacgc
    32881 ataatctgtt tcatctcttc acgctttgca ggctggtaac agtgcaccgg acactgctta
    32941 caggctggtt tctcttcgcc gaacacacat ttatccagcc gcttttgcgc gtaaacaaac
    33001 aacgcctcgt aatgctccgg ctccgctgac gcctgcgggc atttcgcttg ataaagatcg
    33061 atcatttttt taatcgtcag tttttcacga gagatacgct tgccggacat gctgcctcca
    33121 cctcattaag atgcatttat attacatctt aatc
//
LOCUS       DAJTRB010000041        32142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTRB010000041 DAJTRB010000000
VERSION     DAJTRB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.041"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..954
                     /gene="espL1"
                     /locus_tag="OO330_002022"
                     /pseudo
     CDS             1..954
                     /gene="espL1"
                     /locus_tag="OO330_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1126..1233
                     /locus_tag="OO330_002023"
     CDS             1126..1233
                     /locus_tag="OO330_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882897.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1286..2044)
                     /locus_tag="OO330_002024"
     CDS             complement(1286..2044)
                     /locus_tag="OO330_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5882898.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2331..3260
                     /gene="pfkB"
                     /locus_tag="OO330_002025"
     CDS             2331..3260
                     /gene="pfkB"
                     /locus_tag="OO330_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5882899.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3361..3651
                     /gene="ghoS"
                     /locus_tag="OO330_002026"
     CDS             3361..3651
                     /gene="ghoS"
                     /locus_tag="OO330_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5882900.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3757..4617
                     /locus_tag="OO330_002027"
     CDS             3757..4617
                     /locus_tag="OO330_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5882901.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4658..5194)
                     /locus_tag="OO330_002028"
     CDS             complement(4658..5194)
                     /locus_tag="OO330_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5882902.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5341..6009
                     /gene="hxpB"
                     /locus_tag="OO330_002029"
     CDS             5341..6009
                     /gene="hxpB"
                     /locus_tag="OO330_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5882903.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6172..6762
                     /locus_tag="OO330_002030"
     CDS             6172..6762
                     /locus_tag="OO330_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5882904.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6895..8285
                     /gene="tcyP"
                     /locus_tag="OO330_002031"
                     /pseudo
     CDS             6895..8285
                     /gene="tcyP"
                     /locus_tag="OO330_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8289..9105)
                     /locus_tag="OO330_002032"
                     /pseudo
     CDS             complement(8289..9105)
                     /locus_tag="OO330_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9382..9645)
                     /gene="cedA"
                     /locus_tag="OO330_002033"
     CDS             complement(9382..9645)
                     /gene="cedA"
                     /locus_tag="OO330_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5882905.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9828..12089
                     /gene="katE"
                     /locus_tag="OO330_002034"
     CDS             9828..12089
                     /gene="katE"
                     /locus_tag="OO330_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5882906.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12136..12894)
                     /gene="chbG"
                     /locus_tag="OO330_002035"
     CDS             complement(12136..12894)
                     /gene="chbG"
                     /locus_tag="OO330_002035"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5882907.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12907..14259)
                     /gene="celF"
                     /locus_tag="OO330_002036"
                     /gene_synonym="chbF"
     CDS             complement(12907..14259)
                     /gene="celF"
                     /locus_tag="OO330_002036"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5882908.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKKPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14364..15206)
                     /gene="chbR"
                     /locus_tag="OO330_002037"
                     /pseudo
     CDS             complement(14364..15206)
                     /gene="chbR"
                     /locus_tag="OO330_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15214..15564)
                     /gene="chbA"
                     /locus_tag="OO330_002038"
     CDS             complement(15214..15564)
                     /gene="chbA"
                     /locus_tag="OO330_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5882909.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15623..16972)
                     /gene="chbC"
                     /locus_tag="OO330_002039"
     CDS             complement(15623..16972)
                     /gene="chbC"
                     /locus_tag="OO330_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR5882910.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17057..17377)
                     /gene="chbB"
                     /locus_tag="OO330_002040"
     CDS             complement(17057..17377)
                     /gene="chbB"
                     /locus_tag="OO330_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5882911.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17676..18014)
                     /gene="osmE"
                     /locus_tag="OO330_002041"
     CDS             complement(17676..18014)
                     /gene="osmE"
                     /locus_tag="OO330_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5882912.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18216..19043
                     /gene="nadE"
                     /locus_tag="OO330_002042"
     CDS             18216..19043
                     /gene="nadE"
                     /locus_tag="OO330_002042"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5882913.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19273..20160
                     /gene="cho"
                     /locus_tag="OO330_002043"
     CDS             19273..20160
                     /gene="cho"
                     /locus_tag="OO330_002043"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5882914.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20120..20695)
                     /gene="ves"
                     /locus_tag="OO330_002044"
     CDS             complement(20120..20695)
                     /gene="ves"
                     /locus_tag="OO330_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5882915.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20898..21383)
                     /gene="spy"
                     /locus_tag="OO330_002045"
     CDS             complement(20898..21383)
                     /gene="spy"
                     /locus_tag="OO330_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5882916.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21713..22681)
                     /gene="astE"
                     /locus_tag="OO330_002046"
     CDS             complement(21713..22681)
                     /gene="astE"
                     /locus_tag="OO330_002046"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5882917.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22674..24007)
                     /gene="astB"
                     /locus_tag="OO330_002047"
                     /pseudo
     CDS             complement(22674..24007)
                     /gene="astB"
                     /locus_tag="OO330_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24004..25482)
                     /gene="astD"
                     /locus_tag="OO330_002048"
     CDS             complement(24004..25482)
                     /gene="astD"
                     /locus_tag="OO330_002048"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5882918.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25479..26513)
                     /gene="astA"
                     /locus_tag="OO330_002049"
     CDS             complement(25479..26513)
                     /gene="astA"
                     /locus_tag="OO330_002049"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5882919.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26510..27730)
                     /gene="astC"
                     /locus_tag="OO330_002050"
                     /pseudo
     CDS             complement(26510..27730)
                     /gene="astC"
                     /locus_tag="OO330_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28176..28982
                     /gene="xthA"
                     /locus_tag="OO330_002051"
     CDS             28176..28982
                     /gene="xthA"
                     /locus_tag="OO330_002051"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5882920.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29149..29858
                     /locus_tag="OO330_002052"
                     /pseudo
     CDS             29149..29858
                     /locus_tag="OO330_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29863..30540
                     /gene="ydjY"
                     /locus_tag="OO330_002053"
     CDS             29863..30540
                     /gene="ydjY"
                     /locus_tag="OO330_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5882921.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30555..31262
                     /locus_tag="OO330_002054"
     CDS             30555..31262
                     /locus_tag="OO330_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5882922.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31262..31810
                     /locus_tag="OO330_002055"
     CDS             31262..31810
                     /locus_tag="OO330_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5882923.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31820..>32142
                     /locus_tag="OO330_002056"
     CDS             31820..>32142
                     /locus_tag="OO330_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5882924.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8241 a         8241 c         7616 g         8044 t
ORIGIN      
        1 ctcagacctg ggctgatagg tatgttcgca actgtgcaat tgctattctg cccctattac
       61 ctgtgggagt tccaggggct attttcgcgg gtattgcatc acgaatgcca tttgccccta
      121 tacatccatc ggcaatgttg ttaataatgg ctacaggcca gactcaacag cttattacat
      181 tattcaaaca gttgcccata ctccctgaaa aagaaatcat tgaaataata actgcgcaga
      241 atagcgttgg tacacctgct ttatttttgg ctatgatgaa cggacatact gacaacgtga
      301 aaatatttat gcaagaaatt cagtcactgg tagataatca catcattcat gaagataatc
      361 tggttaaatt actgcaaact aaaagtgcta acgaaacacc tggactttat atctccatgt
      421 tgtatggatt cgatgaaata atcgatatct ttctgaatac attaaccact ccaatagcac
      481 taagagcttt taaacaaaaa actggtgatg agtattttag ccatgaaaat acatgatggt
      541 gagccaggat tatacgccgc aatggaaaat aatcaccctt tgtgtgtcac acggttcctc
      601 tctaaaatta atggcatcgc ctttaaatac aagttgagca aagctaacat catggattta
      661 ttaaaaggag ctacagcaca gggaacccct gcattataca tcgccatgag caagggtaat
      721 gaagacgtcg tgttatctta tatatcgacg ctgggtgctt ttgcaaaaaa acattctttt
      781 agtcaacatc agttatttac actattggcc gctaaaaatc atgacaacat gtcagctgtt
      841 catatagcca ttcatcataa gcattataaa actgtagaaa catattatgc tgctataaat
      901 gtcatcagcc aaagcctgag ttttagtgct gatgaaataa agacgtattt ataaccagct
      961 atttacgata tatatcgata cataatttta tttttttcat aaaacgcaga aacagtgacc
     1021 atgtgcacaa cgccacccga cggctgtaat aacaaaaaat cccttgatgc ctgtcccttt
     1081 tgttacactc cgttatcacg cacaagagat atgcaggaca ctggtatgcc gactaaacgc
     1141 tttgataaaa aacactggaa gatggtggtg gtgctactgg caatctgtgg cgctatgttg
     1201 ttgctacgtt gggcagcaat gatttggggc tgagcagtga caaacgaaga cagccagacc
     1261 gcataacacg gtccggcaga taattttaca tgctgtagcc cagcgataag gttgtccgac
     1321 ggtcggtatg ttccggtgca gactctggag gttcagagtt ccatgtgacg ttgtaagcca
     1381 ccttcagtcc aaaatgttca ttaatagcaa catttaatgc gctttcggag ttcagcgttg
     1441 tgtcttccgc gccaaagacc gaaacaccct gcgtaaattt agcgttgtca gtcaactgcc
     1501 atgcataagc cccggaggca tagcccagcg gctgggtttc actggcattg tcggtatatt
     1561 tgtcgtaacg cacacctgga ccgaattcaa agcggaaact gtgtaccggg ccattcaaaa
     1621 actgacgacc ataacccgcg gtcaacacat cgcgctctcg atagccgtta taacggtctg
     1681 tcagccagct tgcctgacca aataaatagt cataatcagt taaattaaag cggctacgcc
     1741 cgcccgccgc atatttttct gaagaacgct catcattaga agaagtatta ctggcgttcc
     1801 cccacagcga ccaggcagtg gtttgtccat accaggtcat ggtggtgtca gccgtaagtg
     1861 aggagctttt cgtattgcct gattgtgcaa gatatcctgc gttcagatta ccttcgaaag
     1921 gttttttagc gctggcagga tcgtccatga cagtaaaaac ggaatcatcg gcagctgcat
     1981 tcagtgacgc aaacatgccc cccgccaaca taacgatggc aggaactgtc ttcaaaagct
     2041 tcatttatca agagtccgta caacaaaaaa agagaccatc gcggtcccgg aaactttctt
     2101 aaggatcaaa gattagcgtc cctggaaagg taacgaatta taaaaaggcg cgaataactt
     2161 agcaatgtat tcttatttca ttttttgaat aagcatgtgg cgaaaacaga tttttattta
     2221 tatatattta tctgcaaaat tttaaataaa gctctaataa atcatattgt taatttcttc
     2281 actttccgct gattcggtgc cagactgaaa tcagcctata ggaggaaatg atggtacgta
     2341 tctatacgtt gacacttgcg ccctctctcg atagcgcaac aattaccccg caaatttatc
     2401 ccgaaggaaa actgcgctgt accgcaccgg tgttcgaacc cgggggcggc ggcatcaacg
     2461 tcgcccgcgc cattgcccat cttggaggca gtgccacagc gatcttcccg gcgggtggcg
     2521 cgaccggcga acacctggtt tcactgttgg cggatgaaaa tgtccccgtc gctactgtag
     2581 aagccaaaga ctggacccgg cagaatttac acgtacatgt ggaagcaagc ggtgaacagt
     2641 atcgttttgt tatgccaggc gcggcattaa atgaagatga gtttcgccag cttgaagagc
     2701 aagttctgga aattgaatcc ggggccatcc tggtcataag cggaagcctg ccgccaggtg
     2761 tgaagctgga aaaattaacc caactgattt ccgctgcgca aaaccaaggg atccgctgca
     2821 tcatcgacag ttctggcgaa gcgttaagtg cagcactggc gattggtaac atcgagttgg
     2881 ttaagcctaa ccaaaaagaa ctcagtgcgc tggtgaatcg cgaactcacc cagccagacg
     2941 atgtccgcaa agccgcgcag gaaatcgtta atagcggcaa ggccaaacgg gttgtcgttt
     3001 cccttggtcc acaaggagcg ctgggtgtcg atagtgaaaa ctgcattcag gtggtgccac
     3061 caccggtgaa aagccagagt accgttggcg ctggtgacag catggtcggc gcgatgacac
     3121 tgaaactggc agaaaatgcc tctcttgaag agatggtccg ttttggcgta gctgcgggga
     3181 gtgcagccac actcaatcag ggaacacgtc tgtgctccca tgacgatacg caaaaaattt
     3241 acgcttacct ttcccgctaa caaaaacatt cccccagcat tgggggaatc accaccaacc
     3301 tgtcggcaac gcgtttctcc gactatgctc aaaagtcatg tgataacaaa ggggtgaact
     3361 atggccagtg gcgatcttgt ccgttatgtc ataaccgtca tgttgcatga ggatacattg
     3421 actgaaatta acgagttgaa taattacctg actcgcgacg gttttttgct caccatgacg
     3481 gatgatgagg gaaatatcca tgagctgggg actaacactt ttggacttat cagtacccaa
     3541 agtgaagaag aaattagaga actggtttcg gggcttaccc aaagtgcaac cggcaaagat
     3601 cctgaaatca ccatcacgac ctgggaggaa tggaatagca acagaaaata aatggttttt
     3661 gggcaataat cagtctgtgg tgtgcgttag ctcgtgtttt tacaccgcat tcttgcgcta
     3721 accttatgat ctggcagaca acatgggaga gacatcatgt ggcaggcaat cagtcgtctt
     3781 ttgagcgagc agttaggtga aggcgaaatc gaactgcgta atgaactgcc tggcggagaa
     3841 gtccatgccg catggcattt gcgctatgca ggacatgact ttttcgtcaa atgtgatgaa
     3901 agggaactgc ttcccggttt taccgccgaa gccgaccaac tggagttact gtcgcgtagt
     3961 aaaaccgtca ccgtgcctaa ggtttgggca gtaggcgctg accgtgacta cagttttctg
     4021 gtgatggatt atctcccacc tcgtccgctg gatgcgcata gcgcatttat tcttggtcag
     4081 caaattgcgc gtttacatca atggagtgac caaccacaat ttggcctcga ttttgataac
     4141 tcgctctcca caactccaca gcccaacacc tggcaacgtc gctggtcaac gttttttgct
     4201 gaacaacgga ttggctggca gttggaactg gcagcagaga aagggatcgc tttcggcaat
     4261 atcgacgcca tcgtcgagca tattcagcag cgtctggcct cgcatcaacc gcagccttct
     4321 ctgttgcacg gcgatttatg gtccggcaac tgtgcgctgg gtccggatgg cccgtacatt
     4381 ttcgaccctg cctgcgactg gggtgaccga gagtgcgacc tagcgatgtt accgctgcat
     4441 actgaacagc cgccacaaat ctatgacggc tatcagtcag tatccccgct acctgccgat
     4501 ttccttgaac gtcaaccggt ttaccaactc tacacgctgt taaatcgtgc aagattattt
     4561 ggcggtcagc atttggttat tgctcagcag tcattggata gattattagc agcatgatat
     4621 gggttgagga taatggccgc tccgtgcggc cttttgatta aataaaccca agcagagaaa
     4681 agaacacata acctgcagca atacaaataa ccggcaggat atatagtgag aaaaactgca
     4741 ggaaaatagt atggtgcgga acaacaatac gggactcaat ttgttcacgc gttagcccct
     4801 cttccccttt ggctttttcc agaatgagtt gatcttcaac accttcccgc aagaagcgcg
     4861 cctggcgact catccgggca ccggaatcct gcaacgccag tccgacaaaa atcaggatga
     4921 aaatcaccca gaacataacg ttcacaccac cattaaaatt aggggtcggg gagttatacc
     4981 agaacagatt caaaaaaggc gtattcgcct gcatcatatc gatcatgaca tgagcaaagt
     5041 cgagcatgac cgcattaatg ccttcctggt tttcctgccg ggtattcata aatttcagca
     5101 aagaaatcaa tgtagagatc aatgcaggta taaaaatcac ccaacccaaa atccttttca
     5161 aaacagcaat gcgtccagct tgttgatacg tcatgagttc tccttgatta agacgcgtcg
     5221 tttcacttag tttacctgta gatatctgtt ttcgcccatt ctttaaaggc gatatgatag
     5281 gcgcttaatc ataagcacgg cttaatacct tacacataat gctctaaagg agaggttgta
     5341 atgtcaaccc cgcgtcagat tcttgctgca atttttgata tggatggatt acttatcgac
     5401 tcagaacctt tatgggatcg agccgaactg gatgtgatgg caagcctggg ggtagatatc
     5461 tcccgtcgta acgagctgcc ggacacctta ggtttacgca tcgatatggt ggtcgatctt
     5521 tggtacgccc ggcaaccgtg gaatgggcca agccgtcagg aagtagtaga acgggttatt
     5581 gcccgtgcca tttcactggt tgaagagaca cgtccattat taccaggcgt gcgcgaagct
     5641 gttgcgttat gcaaagaaca aggtttattg gtgggactgg cctccgcgtc accactacat
     5701 atgctggaaa aagtgttgac catgtttgac ttacgcgaca gtttcgatgc cctcgcctcg
     5761 gctgaaaaac tgccttacag caagccgcat ccgcaagtat atctcgactg cgcagcaaaa
     5821 ctgggcgttg acccactgac ctgcgtggcg ctggaagact cggtaaatgg catgatcgct
     5881 tctaaagcag cccgcatgcg ttccatcgtc gttcctgcgc cagaagcgca aaatgatcca
     5941 cgttttgtat tagcaaacgt caaactctca tcgctgacag aactcaccgc aaaagacctt
     6001 ctcggttaat gaccaggggc agtgatcacg ctgcccctgg ttctttatct gaatttgcat
     6061 tcaactgacg gattaatcgt caatttaaga gaaagagtta caccgtcacc acttccgtgc
     6121 actgtataaa aatcctatac tgtacgtatc gacagtttag tgagttttat catgacggcg
     6181 gaaggtcacc ttctcttttc tattgcttgt gcggtatttg ccaaaaatgc cgagctgacg
     6241 cccgtgctgg cacagggtga ctggtggcat attgtccctt ccgcaatcct gacgtgtttg
     6301 ttaccggaca tcgatcaccc aaagtcgttt cttgggcagc gattaaaatg gatatcaaaa
     6361 ccgatcgccc gcgcttttgg gcatcgtggt tttacccaca gtctgctggc ggtatttgcg
     6421 ctgctggcaa ccttttacct taaggttccg gaaggctggt tcattccggc tgatgcgcta
     6481 caaggaatgg tgctgggtta tttgagccac atacttgccg atatgctgac acccgccggt
     6541 gttcccctgc tctggccatg ccgctggcgt ttccgcttgc ctatcctggt tccccaaaag
     6601 ggcaaccaac tggaacgttt tatctgcatg gcattatttg tctggtcggt atggatgccc
     6661 cattcattac ccgagaacag cgctgttcgt tggtcatcgc aaatgatcaa taccttgcag
     6721 atccagtttc atcggcttat taagcatcag gttgaatact aaaaaggcaa aaatcacctt
     6781 tctggaataa gcaattccat ttgaatataa gagccagctc acagttctgt taatcttgcg
     6841 ccaacactat gactgctacg cagtgataga aataataaga tcaggagaac ggggatgaac
     6901 tttccattaa ttgcgaacat cgtggtgttc gttgtactgc tgtttgcgct ggctcagacc
     6961 cgccataaac agtggagtct ggcgaaaaaa gtgctggtgg gtctggtgat gggtgtggtt
     7021 tttggccttg ccctgcatac catttatggt tctgacagcc aggtacttaa agattctgta
     7081 cagtggttta acatcgttgg taacggctat gttcaactgc tgcaaatgat cgttatgccg
     7141 ttagtcttcg cctctattct gagcgcggtt gcccgtctgc ataacgcatc tcagttaggc
     7201 aaaatcagtt ttctgaccat cggtacgctt ttgtttacca cgctgattgc ggcgctggtc
     7261 ggtgtgctgg tcaccaacct gtttggtttg acggctgaag gtctggttca gggtggtgca
     7321 gaaactgcac gtctgaacgc cattgaaagt aactatgttg gtaaagtctc tgatcagagc
     7381 gttccgcagc tggtcttgtc ctttatcccg aaaaacccgt ttgccgatct taccggagcc
     7441 aatccgacgt caattatcag cgtggtaatt tttgccgcat tcctcggcgt agctgcattg
     7501 aaactgctga aggatgatgc gccgaaaggg gaacgcgtct taaccgctat cgatacccta
     7561 caaagctggg tgatgaaact ggttcgcctt gtcatgcagt tgacccctta cggcgttctg
     7621 gctctaatga ccaaagtggt tgcaggttct aacttgcaag acatcatcaa actgggaagt
     7681 ttcgttgtcg cgtcctacct cggtctgctg attatgtttg ctgtgcatgg cattctgctg
     7741 ggcattaatg gcgtgagtcc gctgaagtac ttccgtaagg tatggcctgt gctgacgttt
     7801 gcctttacca gccgttccag tgctgcgtct atcccactga atgtggaagc acaaacgcgt
     7861 cgtctgggcg ttcctgaatc catcgccagt ttcgccgcct ctttcggtgc aaccattggt
     7921 cagaacggct gcgccggttt gtatccggca atgctgcggt gatggttgcg cctacggttg
     7981 gcattaaccc gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagtt
     8041 ccgcaggcgt tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtgctgcctg
     8101 cgatgggcct gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca
     8161 tgggccgtac ggcgctaaac gttagtggct cgatgacagc tggcacgctg accagccagt
     8221 ggctgaagca aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc
     8281 gttaattctt atgctggcaa cggtccgttt tgtatagggg ccgttgcttt actttttaaa
     8341 tgttccatcg cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg
     8401 tccaacttat caatacattc ctggctcaat gaatgtgcct gtagtgagaa gcttaaaacc
     8461 tcatacccga gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagcaca
     8521 ccacttccca ttcccggaag atttagtgga ttaaaaagaa gattgatatt aagcattgat
     8581 attatcgaag gaaactttag gagactctca ttttttttga atcgcagcaa aaatgctccc
     8641 ggcaggatgt atataatttt tttcatagta tatagtcctt aaataatgaa taaccaacct
     8701 attttttata ttgatcaaat tcgtcatttt ttcgggtcgg aattcacagg taaaatccat
     8761 cccaacaaaa ttctcattaa tatctcttat ccgaaactca ctgccataaa gattattaat
     8821 tatcactcta tatttaacat tgtcctgcag atacgtatca aaactaagtg ctggcgctcc
     8881 tagctttgag gcatcatagt taatgttaga catataggca atcatacgat tgaagttcga
     8941 ctctgctatt tcatcgcagt cacattgacc cgttgatata aagaaagtga gtgccgccat
     9001 ggtagactca agcctttctt taaaagagtc gaaagccagg cttttaatat ttaaatcacc
     9061 ataattactc tgtattaagt ttgtagaaaa catctcccgc ctcatattgt taacaaaatt
     9121 attatcttat ttaaatctaa gtcatttaca atataagttt aagagcgacg ccacaggatg
     9181 aactatcaaa aataactcat catgattagc aaaacttaac cattttaaaa taaataaata
     9241 attaaagaaa aaagatcact tatttatagc aatagctcgt caaaggcagc tttttgttac
     9301 aggtggtttg aatgaatgta gcaacgaaat gcagaatttc aggtcctgta actcccggca
     9361 aaaccgggag gtatgtaatc cttactcagt cacttcccct tcctggcgga tctgatttgc
     9421 ccaacgttgg gcagattcag gcacagtaaa cgccggtgag cgcagaaatg actctcccat
     9481 cagtacaaac gcaacatatt tgccacgcag catccagaca tcacgaaacg aatccatctt
     9541 tatcgcatgt tctggcggcg cgggttccgt gcgtgggaca tagctaataa tctggcggtt
     9601 ttgctggcgg agcggtttct tcattactgg cttcactaaa cgcatattaa aaattagaaa
     9661 aactgtagtt tagccgattt agcccctgta cgtcccgctt tgcgtgtatt tcataacacc
     9721 gtttccagaa tagtctccga agcgggatct ggctggtggt ctatagttag agagtttttt
     9781 gaccaaaaca gcggcccttt cagtaataaa ttaaggagac gagttcaatg tcgcaacata
     9841 acgaaaagaa cccacatcag caccagtcac cactacacga ttccagcgaa gcgaaaccgg
     9901 ggatggactc actggcacct gaggacggat ctcatcgtcc agcggctgaa ccaacaccgc
     9961 caggtgcaca acctaccgcc cctgggagcc tgaaagcccc tgatacacgt aacgaaaaac
    10021 ttaattctct ggaagacgta cgcaaaggca gtgaaaatta tgcgctgacc actaatcagg
    10081 gcgtgcgcat cgccgatgat caaaactcac tgcgtgccgg taaccgtggt ccaacgctgc
    10141 tggaagattt tattctgcgc gagaaaatca cccactttga ccatgagcgc atcccggaac
    10201 gtattgttca tgcacgcggc tcagcagctc acggttattt ccagccatat aaaagcttaa
    10261 gcgatatcac caaagcggat ttcctctcag atccgaacaa aatcacccca gtatttgtta
    10321 ggttctctac cgttcagggt ggcgctggct ctgccgatac cgtgcgtgat atccgtggtt
    10381 ttgccaccaa gttctatact gaagagggta tttttgacct cgttggcaat aacacgccaa
    10441 tcttctttat ccaggatgcg cataaattcc ccgattttgt tcatgcggta aaaccagaac
    10501 cgcactgggc aattccacaa gggcaaagtg cccacgatac tttctgggat tatgtttctc
    10561 tgcaacctga aactctgcac aacgtgatgt gggcgatgtc ggatcgcggc atcccccgca
    10621 gttaccgcac catggaaggc ttcggtattc acaccttccg cctgattaat gccgaaggga
    10681 aggcaacgtt tgtacgtttc cactggaaac cactggcagg taaagcctca ctcgtttggg
    10741 atgaagcaca aaaactcacc ggacgtgacc cggacttcca ccgccgcgag ttgtgggaag
    10801 ccattgaagc aggcgatttt ccggaatacg aactgggctt ccagttgatt cctgaagaag
    10861 atgaattcaa gttcgacttc gatcttctcg atccaaccaa actgatcccg gaagaactgg
    10921 tgcccgttca gcgtgtcggc aaaatggtgc tcaatcgcaa tccggataac ttctttgccg
    10981 aaaacgaaca agcagcattc catccaggtc atattgttcc tggtctggac ttcaccaacg
    11041 atcccctgtt gcagggacgt ttgttctcct ataccgatac acaaatcagt cgtcttggtg
    11101 ggccgaattt ccatgagatt ccgattaacc gcccgacctg cccttaccat aatttccagc
    11161 gtgacggcat gcatcgtatg gggatcgaca ctaacccggc gaattatgaa ccgaactcga
    11221 tcaacgataa ctggccgcgc gaaacaccac cggggccgaa acgcggcggt tttgaatcat
    11281 accaagagcg cgtggaaggc aataaagttc gcgagcgcag cccatcgttt ggcgaatatt
    11341 attcccatcc gcgtctgttc tggctaagtc agacgccatt cgagcagcgc catattgtcg
    11401 atggtttcag ttttgagtta agcaaagtcg ttcgtccgta tattcgtgag cgcgttgttg
    11461 accagctggc gcatattgat ctcactctgg cccaggcggt ggcgaaaaat ctcggtatcg
    11521 aactgactga cgaccagctg aatatcaccc cacctccgga cgtcaacggt ctgaaaaagg
    11581 atccatcctt aagtttgtac gccattcctg acggtgatgt gaaaggtcgc gtggtagcga
    11641 ttttacttaa tgatgaagtg agatcggcag accttctggc cattctcaag gccatgaagg
    11701 ccaaaggcgt tcatgccaaa ctgctctact cccgaatggg tgaagtgact gcggatgacg
    11761 gtacggtgtt gcctatagcc gctacctttg ccggtgcacc ttcgctgacg gtcgatgcgg
    11821 tcattgtccc ttgcggcaat atcgcggata tcgctgacaa cggcgatgcc aactactatc
    11881 tgatggaagc ctacaaacac cttaaaccga ttgcgctggc gggagacgcg cgcaagttta
    11941 aagcaacaat caaggtagct gaccagggtg aagaagggat tgtggaagct gacagcaccg
    12001 acggtagttt tatggatgaa ctgttaacgc tgatggcaga acaccgcgtg tggtcacgca
    12061 ttcctaagat tgacaaaatt ccggcgtaaa tatggataag gtcacgtccc ctggggagtg
    12121 accttaccac cttacttaca catcaagata actacccaac cgataaccac gctcagcaat
    12181 tgcatatttc aacgacgctg atgtcagcac ttccagctcc gttaaccgtg ggaaacagta
    12241 agcactctga cggatagtat tatcgataaa cgccggatga cacatcacct ccagcgaagg
    12301 atcaccccga tggcttgcgt catccagcac ttgcaggaac agtgcctcgc taatctcttc
    12361 accataaaac gcgctactga atccctgcga actgcgcaga ttagccggta aatcaccagc
    12421 gttagatagc ggctgacggt cgatgcgcaa cgcaatcccc tcttcagccg caaacctggc
    12481 aacaatcggg aaaatctgcg ggaacatatg cacatgatga tggctatcaa gatgcgtagg
    12541 tttgcgtcca aatagctcaa tgaaacgcaa atactgactg gcaagctcct gagtaatttc
    12601 ttccagcggt aaagcatctt cttctgccaa ctgccagatc cattttccca gcacaccatc
    12661 gcgggttaac cccggcatag ctgtcagtgg cttgcccata gtaaggacaa agtgcatccc
    12721 tatggccaga ctcggttcat cacgactcaa ctgcaccgca tggtcaatag cctgcccatt
    12781 caccagcgcc gtcgtcgatg tgacaatccc attgcgacag gcctcgataa tgccgtagtt
    12841 ctgacctttg cttaagccaa aatcatcggc attaacaatc agtaagcgtt ccataatcag
    12901 cctcggttaa tgtgcttttt taagctctgc gatgcagtcg gcaaaatttg gcagccattt
    12961 ctcgtgcgcc agaatcatct cgcgtgccag cagctcagca tcgcgatcgg aatgcaccaa
    13021 cggactaagg tttagcgcca gtaacacatc gttaaattct ccgctaagtg cggcgttgct
    13081 ggcagcaatc tcgaagcctt taatggtgtg aatcagcccc atcactttat catcgaaatg
    13141 cgtaatgcgc ggatgtggcg tcgcgccatc gcgccccagc ttacaggtca tttctaccgc
    13201 ccagtctgcc ggaatattat caatatgccc atgatgcggg atattaacgt aatgttcagc
    13261 ttgcttgtcg ttgtagatag cgttgatcac ttcgcacgct gcatcagagt aataagcccc
    13321 accgcgctgt tccagttctt tcggcttaac tttcaactcc ggttttttat acagctcaaa
    13381 aagttgtttc tcgactttct gtactacctg cgctcgtgcg ccgcctttgt agtattcgcc
    13441 catttcaata gccagcatct ctttttgctt gaagtaataa agcaaataag aacacggcaa
    13501 cagattcaga gaacgaatta agccttcgct aaatggcaga tcgaaaatat ttttaacgcc
    13561 agatactttt aactgcccgg atgccacacc atcaagcaat tcggcaaagc gcgacttgcc
    13621 atttaccagc acatccttaa tgaacaccat atggttgagg ccaaacaaat cgatagataa
    13681 atcatcgctg tctttcagca tcagaacatc gtgaataaac atcttcatgc cgatcggaat
    13741 attacacacg ccgataaaac gtttaaatcc ggtatgacga taaacggctt cagtaaccat
    13801 tcccgccggg ttagtgaagt taatcaccca tgcattcgga caaagttctt cgacatcttt
    13861 tacgatgtca aaaatcaccg gaatggtacg cagaccttta aacaaaccgc ccgcgccgtt
    13921 ggtttcctga ccaagataac catgacttaa tggaatacgt tcatccagtt cacgcgccgg
    13981 taattggcca acgcgcagtt gggtagtaac gaaatcagca tctttcaatg cttcgcggca
    14041 atccagcgtt ttataaagct tcatcgggac gccagcgtta tcaatcatcc gttggcagag
    14101 atcgaaaata atatccagtt tagctttgcc accctcgaca tccaccagcc ataattcgct
    14161 gaccggcaat tcgtgataac gcttaataaa tccttccagt aactccgggg tatagctgct
    14221 cccgccacca atagtgacga cttttaattt ctggctcata atttctccct tcagtacaga
    14281 atactgatat ctggcatatc tgcccccccg gacataaata atccagcaac aggacagata
    14341 tgtgaattgt caggtataac gacttactga ttaaattcag tcaatttctt acgatatctc
    14401 ttaggcgtaa aggacgttaa tttcttaaac gttttaataa acaaactcgg gctactataa
    14461 ccggcctcaa acgcaatatc cgtcacggaa tagttggtca tttccagttg tttttttgca
    14521 aaattaatac ggatctcatt aataatctgc attggtgttt tgccataata tcgttgagtc
    14581 gctcgcgtca aatattcctg tgatttggct gacaacgtca ccatattctc cagcgccgat
    14641 tcactaaact gctctttatc atgcatcttt tctaccgtac ttttcagcca ctacggtaca
    14701 tcatcaatca cctgttcttc gcggtaatga cgtaaacggt taatgacata aaaagtaacc
    14761 atctcaacaa actcttccag ccctgtttcg cggaaattta atgaagaaat cactgtttcc
    14821 acataggtga gaaaagcgtt attggtccgg tatacctgcg aagcaacaaa gcaataaggc
    14881 aacaacggca ggtaatgctg ctcaaaaaag cgtttactga tcccaacgtt caatatgcgc
    14941 gtggcaccaa actcataaaa actttgatgg tgcgaaccta acggaataaa aacaaaatcg
    15001 ccccgttcca gtaacacgcg cttaccgtta atctcctaga aataacgccc ggttaatact
    15061 agagtaaatt cataatagtc gtgctgatgc agtccgctga tactctcagt tttgttatag
    15121 ataaacacat ggaagttttt gccattaaac aactgctgtt ctcgggcagt ggcaatttcc
    15181 ggcgcgttaa tcactggctg catcatcgac tccttatgcc ttcagttttt catgaagctc
    15241 aattaattca gtaatcagtt cacgcgcaag catggacgtc attaaatgat cctgagcgtg
    15301 gaccagcacc agactcactt tcatcttacc ttcgcccgca tcgccttcaa tcagtttcgt
    15361 ctgtaccaga tgcgcttcat tcaatgccat ccgtgactga tccatcatgg ctttcgctgc
    15421 ggcaaaatcg ccctgcttcg cctgtttcaa tgcggcatac gccaggctac gcgcttgtcc
    15481 agagttgatg ataagcccca tcaccacttc ttccagctct tcagcttctg tttgcgtatc
    15541 aggaatatta tcgagatcca tcatacatcg ttcctctctc ttcttaccgg caagattacc
    15601 cgtaccggca tcgattaaaa tttcaggggt tagcgatatc ttcttcgctc tcttctttat
    15661 caatcgcatt ttgtgctttg ttagccacca caacaaaggg cagataaatt aacgttgcga
    15721 tgccaaggtt gaagagtgcg accagcaatg cggcgacgct accgttggtg ttaaagaagg
    15781 ctcccagacc ggttggcatg gtccacggtg caatattggt caccggagga atgatgccca
    15841 tgtagtacgc tgcgagggtg attgccgcca gaatcggttg taccagtaca aacgggataa
    15901 acatcaccgg gttcatgata attggcagac caaacagaat cggttcgtta atctggaaga
    15961 tgccggacgg cagcgccagt tttgccacct gacgataatc agcacggcga gaggcgataa
    16021 agatagccag gatcaggcct aaagtcgcac cactgccccc aaggaaaata aaggagtcca
    16081 gcatcggctt ggcccagata tggaaggtct taccggctgc cagcgccgct tcgacggaac
    16141 catattgctg ataggtcgcg atattttcca gcgcccacgg cgtcataatg ccgttgtcca
    16201 gtgcggtcag cgccagcgcg ccatgaatac cgaagaacca gagcagtgga acaaagatca
    16261 cataggccca gcccaccacg ctacccaacg atgccagtgg ggttgagatg gtatccataa
    16321 tgatctgatg gaagttggtg ccccaggtat tcaacgccca ggcaataatc cccatcacgg
    16381 aaagaataat aaagccggga attaatgccg agaaagaacg cgatactgaa gcaggtacgc
    16441 tgtcgggcag tttaatgacc caattgcggc ggacaataaa ggtaaacatt tctgccacca
    16501 ccaagccaat aataatcccg gagatgatat tcgccccacc taaccagttt gcgccaaccg
    16561 cataggcctc accgacacta tatggggtga cggtcataaa tgctgcaacg gataacaacc
    16621 cagccgccag cgcatcgact ttacgctctt ctgccagcgc catgccaata aagaacggtg
    16681 ccattaaaga cattattcct aatgttccgt tatatacgtt gccgccaata cctttcagac
    16741 cattaagtgt ttcaatggtt gaggcgtcga ggcgaatacc taaggaataa aaaaacgacc
    16801 cctccccaaa gcttagaaaa acgttgttaa ttaatacaaa catggccccc gcaagggtta
    16861 acggcattaa gcgaataaag ccatttttga ttgcattaac gtgtggctgc tttcctattt
    16921 taactgcaaa agggaggagt accttttcaa gcgatgcaat aacattactc atagaaaaat
    16981 acccttaaaa accgcaattt aaatattacg gtattgattt atgaaataac tctttgacgg
    17041 gaaaatttaa aataaattaa tttgctgcgg cttttttaat cgctgcaacc gcagccttaa
    17101 gcacgcctaa accatcgact ttgccataaa gcagcgagtc aattacttca accggtttgt
    17161 tgggtaacaa acgctggatt tcgggcaaca tataagcaat ctgcggccct aataacacga
    17221 catcggcatt ctgacctttt tcaccagcca gtgtttccgg aaatgcttca ataatgaccg
    17281 gaacttcata tttttctgcc tgtgcgcgca tttttgatac cagtaaagag atagacatgc
    17341 ccgcagaaca aaacagataa atgtgtttct tttccataaa actgccctca tcgacgatta
    17401 tctgtcagcc agacactccg caagccttga cctgcttcca tgctctgggt aacttgcgaa
    17461 accaacatga ctttttttgt ggctgaaagg agtatacggt atagacctga gcaatagtat
    17521 ttctttgacc ttataaattg tttctccttg acctgtggtt atgaccctct tcacatttcg
    17581 ggcaaataat tcgcgaagat aattaagaac tcaggccaaa aaaaaccggc gcaatggccg
    17641 gtttccgttg ttactcaagc tttcagacga attgattact tcgcagcctg tggatcagtg
    17701 tcgtattcag cacaggtctg ataaccggag ttgatgacat gtccggtatc atctaacgcg
    17761 acaaagtagg tttctgcttt accatcacgt tgacccagga tgtaggtctg gcaagtaccg
    17821 cgagcatgga tcatgctcac ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc
    17881 cggctcatgc cttttttgac gtctttcacc acaggctgta caaactggtc tttggtacga
    17941 tcataagctg tacaacccgc cagcatggtt aataccgccg ctgcactcag aattcctgcc
    18001 atattcttgt tcatattccg tcctcttgtt tatcagcgtg ttagataagc ctggaataca
    18061 ttgggcgctt tttcaagccc gtgaacgaaa cggctccgct ttcagaggat tcctgtatga
    18121 cgttttaacc accattcagc ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt
    18181 aaggaagttt tgtcttttct gtctggaggg gttcaatgac attgcaacaa caaataataa
    18241 aggcgctggg cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt agtgtcgatt
    18301 ttctgaaaag ctacctgcaa acttatccgt tcattaaatc actggtgctc gggatcagcg
    18361 gcggtcagga ctccacgctt gccggaaagc tgtgccagat ggcgattaat gagctgcgcc
    18421 aggaaactcg caacgaatca ctgcaattta ttgccgtacg cctgccctat ggtgttcagg
    18481 ctgacgaaca agattgccag gatgccattg cctttattca accggatcgc gtattaaccg
    18541 ttaatatcaa gggcgcggta ttggctagcg agcaggcatt gcgggaagca ggcattgaac
    18601 tgagcgattt tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca caatatagca
    18661 ttgcgggtat gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg
    18721 gattcttcac taaatatggt gacggcggta cggatattaa tccgctgtat cgtctcaaca
    18781 aacgccaggg taaacagtta ctgacggcat taggttgccc ggaacacctt tataagaaag
    18841 ccccaactgc cgatcttgaa gatgatcgcc cttctctgcc agatgaagtg gcactcggcg
    18901 tgacctatga caatatcgac gactatctgg aagggaaaaa cctacctgaa caggtcgcca
    18961 gaacaataga gaactggtat ctgaaaaccg aacataaacg ccgtccgcca attaacgttt
    19021 tcgatgattt ctggaaaaag taataatttg cacctattgg attgtgcgaa aaagagtaat
    19081 ttgttcacgc cggatgcggc gtgaacgcct tattcgacct acaaaactat gcaaattcaa
    19141 tacattgcag gagtcgaata ggcctgacag gcgtagcacg tcagacggtg taacctttgt
    19201 catcaacccg cctctttttt aatcgcctcc cgcctgttac actggataga taaccagcat
    19261 tcggagtcaa cagtggtacg gcgtttaact tctccgcggc tcgaatttga agctgcggca
    19321 atttatgaat atcccgaaca tttacattca ttccttaatg acttacccac ccgaccaggg
    19381 gtgtatctgt ttcatggcga aagtgacacc atgccgctct atatcggcaa aagcgttaat
    19441 atccgcagtc gggtgctttc tcatttacgc accccggatg aagccgccat gctacggcaa
    19501 tcccggcgaa ttagctggat ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg
    19561 cgattaatca aagaacaaca gccgctgttt aataaacggt tgcgccgcaa tcgccagctc
    19621 tgtgccctgc aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt
    19681 tcacgagccc ccaacctgtt tggcctgttt gccaataggc gcgcagcttt gcaagcattg
    19741 cagagcatcg ctgatgaaca aaaactttgt tatggcctgt tgggactgga accgttaagt
    19801 cgcggtcgtg catgttttcg ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa
    19861 gagagccatg aggaacatgc gctacgcttg cgccagtctc tggagcgttt gcgagtggtg
    19921 tgttggcctt ggcaaggtgc ggtggcactg aaagaacagc acccggaaat gactcaatat
    19981 catattattc aaaactggct gtggctgggg gcggttaatt cgctggaaga agcgacaacg
    20041 ttaattcgga cacccgccgg gtttgatcac gacggttata aaattctttg taagccgctg
    20101 ctttccggta actatgaaat tactgaactt gatccggcga atgaccagcg agccagttga
    20161 tttcactgaa caacagttta ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga
    20221 accagcacgc gccctggtcg gtagtgagca gtttgtcgcc cagttgccac gccccgttaa
    20281 tcacaaacac caccccgccg cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga
    20341 ctttcgcctt acagacatcc aggcgcgtca taatattgaa atccatcgac atctgccctg
    20401 ccgtcagttt cgcttttacc acctggtccg ccgtaaaggc aaaaggctgt aacggtttta
    20461 aggtatggtt aaagcggtct gcgctttcaa ggagcatctc gccgccttcc agcaacgtca
    20521 ctatcctttc catgccggga aacaaagaaa actccccatt cgccgcgatg gatgcaatgc
    20581 tggcacgcca gtaaaaatca cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac
    20641 cggcagcgtt tcgccacaga ttcaccgaca ttttacgcat atcaaagtat tccatactcg
    20701 ctcccggccc gttcttttca ttgttatcgt tcaggtaacc gtggatggtt atcggcaact
    20761 cgggtgattt acgttagtgg tgatcaggct ttttttacaa ctgtttgaga agagaaaaga
    20821 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa
    20881 gttttttggc tgaaagatta ttcagcagtt gcaggcattt taccttttgc cgctggacgt
    20941 tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg cgtcaggatg
    21001 ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc
    21061 atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc aatgatgtca
    21121 tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg gcctttcatg
    21181 atttcgtgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc
    21241 tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc cggtgctgcg
    21301 gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt agaggcaaca
    21361 aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt attacggctt
    21421 tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt caattaatca
    21481 gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa
    21541 agaaaatttg atggagagtg atgagagaat attacaacac gatgattttg cagagattat
    21601 gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt aatctgtatt
    21661 aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag tattagctta
    21721 ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta gggaataaca
    21781 catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc
    21841 ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg aaggacggcg
    21901 agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc acgataccga
    21961 cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac tggcgaagat
    22021 cgttttgccc aaagggcaac gctttgccaa gttccaacgt acaggccagc gcgccaaaat
    22081 gtctgacgct gaaatgggta aacgtaccac caggctcctg atggaacacc agcgcctcca
    22141 gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta
    22201 atacaccgaa ctgcggatgc aaggagccac gaattgcggt atgtagatca aggtgccaac
    22261 gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt tcgcgcgccc
    22321 gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt cggttcatat
    22381 cgctatggca ataacgtttc ccttgcttca gcgcaggagg attcccgagg atcaccagca
    22441 accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg tccagcattt
    22501 ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg ccttgcggtg
    22561 gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga acgccgttga
    22621 tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga aaattatcca
    22681 ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa ttggctcaat
    22741 acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc ggcagcagta
    22801 agacgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacaa ggtatcgttc
    22861 atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaataccac ccgcaaccgc
    22921 aggcacgccg ggccgccgcc attcgccatg ctttcacgta agtcaaagac ttttagttcg
    22981 ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc ggcgtgttcc
    23041 cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa cagttggctg
    23101 ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg aacttctatc
    23161 gccataaaac cgttcacccg cgtgccagta actgtgactg gcgagcgaac gcctgttggt
    23221 ggcaaaacag cacctggcgg ttactcacgg caatcacgtc attatgaaaa acgctctggt
    23281 cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg gggattcacc tgattaagcc
    23341 ttgccaccac ctcgctggct tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat
    23401 cattgccttc ttctcgcccg tagacaaaaa gtagcatacc cggttcaccg taatgaccgc
    23461 cgagacgatt gtggtttgcc gccccctcat caccgagcaa cgctacctgc ggcaacgccg
    23521 aatggacgct aaatttctct tcgtccttaa aaatcgcttt taacagcgat tcagtgacgg
    23581 gcgcttccag cgaacggtga aatttattgt tcaggttggc aacggtgaga tgcactttgc
    23641 catccagcgt atcggcagat ggcgcgatcg ttgccgcatt ggctacccac attggcgaag
    23701 cggaactgac gctggaaagc cagtgcggtg cctggcgtgc aactttttcc agtacctgct
    23761 catcgctacc actgaatccc aactgacgca gcaccggaat aaacggacgc tcgtgcggcg
    23821 ggatcacggc ctgggggaat cccgcatcgg caagggcttt cattttcagt aagccctgtt
    23881 ttgccgccag tcgcgggtta gacacctgaa aacggtgacg ggtagaggct tcattaccaa
    23941 acgacaggcc cgcgtaatga tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg
    24001 cgttcatcgc accacctcat cggaaaaatc cagcccgggg ttaagcgtgg cgggcaatgt
    24061 taacgagtcc gactccaggc tcgccatagg ccatgcgcag taatctgcgg cataccaggc
    24121 gctggggcga tggttaccgg aagcaccaat gccgccgaat ggggcggtac tggcagcacc
    24181 agtaagcggt ttgttccagt taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa
    24241 tttttcccgc tcgggggaaa ccagaccgca aaagagtccg aagcgagtgt tgttcgccat
    24301 ccgaatcgcc tcatcaaaag tatcataacg ccagacgcgc aaaagcggcc cgaacacctc
    24361 ttcatccggc acaccgccaa cgcccgtcat ttcaatgatc cctggtgtca gcaacgatgt
    24421 ccctgcttgt aataagcgcg gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg
    24481 ccaggcagta accacctgct gtgcggcctg ttcagaaatc agcccgccaa taaacggctg
    24541 cggttcgtca tcccagttgc ccggcgttaa tcgctggctg acggcaacca gactagcaag
    24601 aaacgcatcg ccctgcgctc cgcttttgag caataaacgg cgggcgcagg tgcagcgttg
    24661 cccggctgtg acaaacgccg actgaatggt cagatggaca gccgcgtcga tatccgccac
    24721 ctcatcgata attagcggat tattaccgcc catctcaagg gcgagaattt tctccggctg
    24781 accggagagc tggcgatgca gctggtagcc ggtattggcg ctaccggtaa acagcaaacc
    24841 gtcgagatcc tccagcgcac tcagcgcctg acccgtttca cgcccgccct gcaccaggtt
    24901 cagcacgccc ggcggcaagc cagcctgctg ccataaacgc attaccgctt cgccactcca
    24961 cggtgtcagt tcgctgggtt taaagataat ggtgttacct gccagcaatg ccggaacgat
    25021 atgtccgttc ggcaaatgac cagggaaatt atacggccca aacaccgcca gcacgccgtg
    25081 cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca ctacgctgct cgccggtacg
    25141 aacgtgatac gccttaattg atatcgcgat tttattgatc atcgccgtca cttcggttgc
    25201 cgcttcccag cgcggcttac ccgtttctct ggcaataatc gcggttaatt cggctttatt
    25261 gctttccagc aatccggcaa agcgttcgac aacgacctga cgttcagcca atgagagccg
    25321 cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc tgttcgacct gagcggcatc
    25381 ggcatcattg ccttgccata acacctcgcc cgataccgga ttacgcttca cacgcgatgc
    25441 gccctggccc gttatccagt caccgttaat ccataaagtc atgctgtttt ctcctctgcg
    25501 cacaggcgca ccagacgaac gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg
    25561 gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg
    25621 tgataatttt cattggcgac caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct
    25681 gccacttcca ccagccgact tttacggatg gcgcgcacgc ggtcgatgtc acactcaagc
    25741 gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac gaaaaccttc tttctccagc
    25801 accgcgcggg caggcgcggt ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg
    25861 gataaaaagt gggtatagat cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc
    25921 ccggtgccgc agagaaaatc ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg
    25981 ctttgccaga acggtgaata gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact
    26041 ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa agcgcgattt cgacagcaaa
    26101 tagccgttgc cctctttgcg ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg
    26161 ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt tatagacatt cagctctttt
    26221 gaggcgtgaa ccaacgtgcc gacgcgatag ttgtaccagg gatcgttcag cccaaccgcc
    26281 acctcaatag cgcaaatccc cgccacggtg cctgtctcgc tatcttccag cacgaacaca
    26341 tagccctgct cgcttttggg cagttcacct tgccaggttt tgattgccct ttcgatacgc
    26401 gccgaaagcg tggcttcatt ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca
    26461 agctgcatca gcgccgagac atctgagcgc tcaacgggac ggatgaccat catgatgaac
    26521 ctcggctaac aaagtgttcg caagcagctg caaagcgatc cagtccggtc gtcacctctt
    26581 cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt gccacccgca atcagtacca
    26641 tcacgcctgc tttcgccgct tccagagaga tctgtttcgc ttgcccggcg taatcggcat
    26701 tcagtacaca gccaatcaac aaacctaagc cgcgaacttc actgaacaaa ccatagcggt
    26761 gattaatagt attaagacgc tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca
    26821 tctctggtgt gttgatgagc tccagcactt tgcctgctac cgccgaggcc agcggattac
    26881 cgccataggt ggtgccatga gtgccaacgg tcatcacgcg ggcgcactct tcggttgcca
    26941 acaacgcacc gaccgggaaa ccgccgccca gcgctttggc ggtggttaac agatcgggcg
    27001 tcacgccgta gtgcatatag gcatacagct caccagtgcg cccgacgccg gtttgtacct
    27061 catcaaaaat cagcaaggca ttgtggcggt cacacaattc acgcagacct tgtaaaaacg
    27121 cgttgctggc tggcaccaca ccgccttcgc cctagatggg ctcgacaatc accgcacagg
    27181 tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt atatgcagca tgacgaatat
    27241 ccgccggcag tggcgcaaaa tcctgtgaat aggctggctg cccacccgca ctgacagtaa
    27301 acagcgtgcg accatgaaac gcatttttga acgccacgat gccactctta tggctgccgt
    27361 agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg
    27421 agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat caattttttc gccagtcgca
    27481 gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa cttactcgcc tgttcgttca
    27541 gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga
    27601 agtcgatata ctctttcccc tgctgatccc acaagcgcga accttcgcca cgtaccggta
    27661 taaagggtgc cggagcgtaa acaggtatca tccattcatc aaagttttca cgcgtaattg
    27721 gctgagacat agcgacctct acagtaaata attcgttatt tgtatgttaa caataagtaa
    27781 tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc cagtgataaa agtgcataaa
    27841 cggcggaggc taactggaaa tcaaggagtt ataaccaaac catatgcatt taaagtgcat
    27901 ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc
    27961 agaagtaaaa tataattcta gaattgtgat cattgacgaa atttactgga aattactgcg
    28021 ccattctgac gcagcgcgca ccaaaagcgg gcattttttg cgccatcgtt gacatcatta
    28081 acaaccatcg atcaaatcac ttaacaacag gcggtaagca acgcgaaatt ctgctaccat
    28141 ccacgcactc tttatctgaa taaatggcag cgactatgaa atttgtctct tttaatatca
    28201 acggcctgcg cgccagacct caccagcttg aagccatcgt cgaaaagcac caaccggatg
    28261 tgattggcct gcaggagaca aaagttcatg acgatatgtt tccgctcgaa gaggtggcga
    28321 agctcggcta caacgtgttt tatcacgggc agaaaggcca ttatggcgtg gcgctgctga
    28381 ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg tgacgacgaa gaggcgcagc
    28441 ggcggattat tatggcggaa atcccctcac tgctgggtaa tgtcaccgtg atcaacggtt
    28501 acttcccgca gggtgaaagc cgcgaccatc cgataaaatt cccggcaaaa gcgcagtttt
    28561 atcagaatct gcaaaactac ctggaaaccg aactcaaacg tgataatccg gtactgatta
    28621 tgggcgatat gaatatcagc cctacagatc tggatatcgg cattggcgaa gaaaaccgta
    28681 agcgctggct gcgtaccggt aaatgctctt tcctgccgga agagcgcgaa tggatggaca
    28741 ggctgatgag ctggggggtg gtcgatacct tccgccatgc taatccgcaa acagcagatc
    28801 gtttctcatg gtttgattac cgctcaaaag gttttgacga taaccgtggt ctgcgcatcg
    28861 acctgctgct cgccagccaa ccgctggcag aatgttgcgt agaaaccggc atcgactatg
    28921 aaattcgcag catggaaaaa ccgtccgatc acgcccccgt ctgggcgacc ttccgccgct
    28981 aatttagcag ctctcctggc tcaaactggg tcaggagaat taaccttgag aaaaatcaac
    29041 aaactgtcag taatgatttg ttgcctgccg tcctttgtta taccgtcact gcgtttttag
    29101 ttgtctgacc acttctctat tatcaagttt gatataggaa actccacgat gaacgctgag
    29161 cgtaactttc tgtttgcctg tcttattttt gcgctggtca tttacgctat ccacgctttc
    29221 ggtttattcg atctgctcac cgatttaccc cacttacaga cactcattcg ccagagcgga
    29281 ttgttcggtt atagcctcta tattctgtta ttcatcaatg ccccctcttt ctgttaccag
    29341 gaagcatact ggtgatcgct ggaggaatag tttttggccc gctcttaggg acacaactct
    29401 cattaattgc cgcaacgctg gcctcctcgt gctcattcct gctggcgcgc tggctgggac
    29461 gtgatttact gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca
    29521 ttgcgcgtaa cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca
    29581 atattcaaaa ttacgcttac ggattcacca ccatcgcctt ctggccttat acccttattt
    29641 cggcactcac gaccctgccc ggtattgtta tttataccgt gatggcaagc gatctcgcca
    29701 atgaaggcat tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta
    29761 ttctcatcca gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc
    29821 gccgcagccc acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca
    29881 gtgtcatgga aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc
    29941 tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa
    30001 ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt
    30061 ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa
    30121 tcgctgttta tggcttatgc gcccccgaaa gcattttatg aagccctgaa agaggcaggt
    30181 ggtacgccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc
    30241 aaactggata tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg
    30301 attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca
    30361 gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc
    30421 aatgcaacat acacttatgg tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat
    30481 gcctcagttc tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa
    30541 agccaagata aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc
    30601 tcatcatcct gttgttcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt
    30661 ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta
    30721 ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca
    30781 ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt
    30841 tctggggcgg cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta
    30901 tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca
    30961 gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc
    31021 cttttgtccc tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct
    31081 cgttttttat cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcag
    31141 gcagcatgtt aacaggcggt actttctggt ttgtcaccga actgtttatt ctgtttgccc
    31201 tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact
    31261 gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca
    31321 tcgtcgccat tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat
    31381 cttttatttg gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc
    31441 ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt
    31501 ggatatcacc agtatgaaag tcgccgagcg taccggcagc accgataaac tgctggcagt
    31561 ggctgactgg cggcaaagcc cgctctttag cgatgaagaa cggctggcgc tggagtacgc
    31621 cgaagccgca agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc
    31681 gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc
    31741 ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa
    31801 acgttcttaa ggagggaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt
    31861 ttctttggca acacatgcca gtgactggca ggaaattaaa aatgaggcca aagggcaaac
    31921 cgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg actgggttag
    31981 cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctcg cggatgccgc
    32041 agacgcggtg aagcgcattc agaccgaagc tgctgccggt cgtaaaacgg gcggctcggt
    32101 ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta aa
//
LOCUS       DAJTRB010000042        31776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTRB010000042 DAJTRB010000000
VERSION     DAJTRB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.042"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..1370)
                     /gene="yeaG"
                     /locus_tag="OO330_002057"
     CDS             complement(<1..1370)
                     /gene="yeaG"
                     /locus_tag="OO330_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5882925.1"
                     /translation="MNIFDHYRQRYEAAKDEKFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYDRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHL
                     FYVLEQ"
     gene            1806..2552
                     /gene="mipA"
                     /locus_tag="OO330_002058"
     CDS             1806..2552
                     /gene="mipA"
                     /locus_tag="OO330_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5882926.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2642..3403
                     /locus_tag="OO330_002059"
     CDS             2642..3403
                     /locus_tag="OO330_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5882927.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3454..4338)
                     /locus_tag="OO330_002060"
     CDS             complement(3454..4338)
                     /locus_tag="OO330_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5882928.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4422..5417)
                     /gene="gapA"
                     /locus_tag="OO330_002061"
     CDS             complement(4422..5417)
                     /gene="gapA"
                     /locus_tag="OO330_002061"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5882929.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5759..6172
                     /gene="msrB"
                     /locus_tag="OO330_002062"
     CDS             5759..6172
                     /gene="msrB"
                     /locus_tag="OO330_002062"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5882930.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6214..6486
                     /locus_tag="OO330_002063"
     CDS             6214..6486
                     /locus_tag="OO330_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5882931.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6856..7932
                     /locus_tag="OO330_002064"
     CDS             6856..7932
                     /locus_tag="OO330_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5882932.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7959..9338
                     /locus_tag="OO330_002065"
     CDS             7959..9338
                     /locus_tag="OO330_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5882933.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSTNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9355..10400
                     /locus_tag="OO330_002066"
                     /pseudo
     CDS             9355..10400
                     /locus_tag="OO330_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10421..11257
                     /locus_tag="OO330_002067"
     CDS             10421..11257
                     /locus_tag="OO330_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5882934.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11262..12209
                     /locus_tag="OO330_002068"
     CDS             11262..12209
                     /locus_tag="OO330_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5882935.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12219..13199
                     /gene="ydjG"
                     /locus_tag="OO330_002069"
     CDS             12219..13199
                     /gene="ydjG"
                     /locus_tag="OO330_002069"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5882936.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13336..14094
                     /locus_tag="OO330_002070"
     CDS             13336..14094
                     /locus_tag="OO330_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5882937.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14212..15570
                     /locus_tag="OO330_002071"
     CDS             14212..15570
                     /locus_tag="OO330_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5882938.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15663..16304)
                     /gene="pncA"
                     /locus_tag="OO330_002072"
     CDS             complement(15663..16304)
                     /gene="pncA"
                     /locus_tag="OO330_002072"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5882939.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16315..17331)
                     /gene="ansA"
                     /locus_tag="OO330_002073"
     CDS             complement(16315..17331)
                     /gene="ansA"
                     /locus_tag="OO330_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5882940.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17498..19354)
                     /gene="sppA"
                     /locus_tag="OO330_002074"
     CDS             complement(17498..19354)
                     /gene="sppA"
                     /locus_tag="OO330_002074"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5882941.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19515..20066
                     /locus_tag="OO330_002075"
     CDS             19515..20066
                     /locus_tag="OO330_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5882942.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20183..21226
                     /gene="selD"
                     /locus_tag="OO330_002076"
     CDS             20183..21226
                     /gene="selD"
                     /locus_tag="OO330_002076"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5882943.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21231..23192
                     /gene="topB"
                     /locus_tag="OO330_002077"
     CDS             21231..23192
                     /gene="topB"
                     /locus_tag="OO330_002077"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5882944.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23319..24359
                     /locus_tag="OO330_002078"
     CDS             23319..24359
                     /locus_tag="OO330_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5882945.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24476..25815)
                     /gene="gdhA"
                     /locus_tag="OO330_002079"
                     /pseudo
     CDS             complement(24476..25815)
                     /gene="gdhA"
                     /locus_tag="OO330_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
     gene            26051..26323
                     /locus_tag="OO330_002080"
     CDS             26051..26323
                     /locus_tag="OO330_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5882946.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26289..26696)
                     /gene="nudG"
                     /locus_tag="OO330_002081"
     CDS             complement(26289..26696)
                     /gene="nudG"
                     /locus_tag="OO330_002081"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5882947.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26783..27403
                     /locus_tag="OO330_002082"
     CDS             26783..27403
                     /locus_tag="OO330_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5882948.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLTFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27412..28719)
                     /gene="ynjE"
                     /locus_tag="OO330_002083"
                     /pseudo
     CDS             complement(27412..28719)
                     /gene="ynjE"
                     /locus_tag="OO330_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28786..29439)
                     /locus_tag="OO330_002084"
     CDS             complement(28786..29439)
                     /locus_tag="OO330_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5882949.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29439..30956)
                     /locus_tag="OO330_002085"
                     /pseudo
     CDS             complement(29439..30956)
                     /locus_tag="OO330_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30929..31776)
                     /locus_tag="OO330_002086"
                     /pseudo
     CDS             complement(30929..31776)
                     /locus_tag="OO330_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7963 a         7606 c         8241 g         7966 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atcgtagtca cgatattcct gatacgactt ggctttcgga
      181 tcggtgtctt tcagactttc gccatcataa acccgcatct ttgaataaat gctggagttt
      241 tctggctctt tcaggcgcga aagaatggaa aaacgtgaca gtgtttcgag cgtgccaggg
      301 gcgcatgggg cgtgagtcaa ttcactgtga ttaagcaatt tctcgtagat tttgatctct
      361 tcggaaatgc gcaagcaata cggcaccttc acgatgtaaa cacggtcgag gaaggcttcg
      421 ttgtttttgt tattacggaa agtgacccat tcggacttgt tcgagtgggc gagaataatc
      481 ccgttgaacg gcagggcgga gataccttcc gtcccgttgt agttaccttc ctgagtggcg
      541 gttaacaagg gatgcagcac ttttatcggt gctttaaaca tctcaacgaa ttccatgatc
      601 ccctgattgg cgcggcacag cgcaccggaa tagccgtagg cgtccgggtc attctgcgcg
      661 tagtgttcga gtttacgaat atcgactttc ccaaccagcg cggagatgtc ctggttgttc
      721 tcatcaccgg gttccgtttt ggcgatagca atttgttgca gaattgacgg ccagaccttc
      781 actacccgga acttagtgat atcgccacca aattcatgca ggcgttttgc cgcccatggc
      841 gacatgatgg tgccgagata acggcgagga atgccatact ctttttccag aatctgagca
      901 tcttcctgcg gattgaaaag acagaacgga tgatcgttga ccgggctacg ctcaccgttg
      961 gcgctcaata cataaatcgg tacgagctgc attaatgatt tcagtcgctc agcaagcgat
     1021 gatttacccc cacccacagg ccccagcaga tacaggattt gtttcttctc ttccagcccc
     1081 tgagccgcgt gtttcagata agagacaatc tgttcaatcg cgtcttccat gccgtaaaac
     1141 tcttcaaatg ccggataacg tgcaatgacc cggttagaaa agagtcgaga aagtctgggg
     1201 tcctgggctg tatcgaccat gacaggctca ccgatagcca tcaatagccg ctcagccgcg
     1261 ttggcataag cactgcgatc ttgccgacaa gtggtaagaa actcctgcag tgtgaacttt
     1321 tcgtccttgg cagcttcata tcgctggcga tagtgatcga atatattcat ggtatgccgt
     1381 cctttcgttt tttagcacag gttaagagcc gttcatatga atagtagagg ctcccggaag
     1441 aggtaaactg aacgacgtct cttgctaaaa cacagcaact ctcatgccat tttatcgacg
     1501 atgagaacaa cgtgcccggt gatacacctt caaaattaag cgtagatggc aaatggaaaa
     1561 cttgcctgca cggctttctt aatttcaatg gcatatcaac aactcatcca taaattttgc
     1621 ataattaatg taaagaccag gctcgccagt aacgctaaat ccatttggct gtaagcgcgg
     1681 tgtcatccgc ggcaggaaaa ttaaacagtt actttaaaaa atgaaaacgt aaaaaggttg
     1741 ggtttcgatg tattgacggg taaactttgt cgcccgctaa acatttgttt aaggaatgat
     1801 taattgtgac caaactcaaa cttctggcac ttggagtgct tatcgcaacg tctgcaggcg
     1861 tagcgcacgc tgaaggtaaa ttttccctgg gcgcaggcgt aggtgtcgtt gagcacccat
     1921 ataaagatta cgataccgat gtttacccag taccggtaat caactatgaa ggcgataact
     1981 tctggttccg tggcttaggt ggtggttact acctgtggaa tgacgcaacg gataaacttt
     2041 caattaccgc ttactggtcg ccgctttact tcaaagcgaa agacagtggc gatcaccaaa
     2101 tgcgtcacct ggatgaccgt aagagcacca tgatggctgg tctgtcttat gctcacttta
     2161 cccagtacgg ttacctgcgt accaccctgg ctggcgatac cctggataac agcaacggca
     2221 ttgtctggga tatggcctgg ttgtatcgtt acaccaacgg tggcctgacc gtgactccgg
     2281 gtattggtgt gcagtggaac agcgaaaacc agaacgaata ctattatggc gtatcgcgca
     2341 aagagtccgc tcgcagcggt ctgcgtggct ataacccgaa tgacagctgg agcccttacc
     2401 ttgagctgag cgccagctac aacttcctcg gcgactggag tgtttacggt accgcgcgct
     2461 acacccgtct gtctgatgaa gttactgaca gcccgatggt ggataaatcc tggactggcc
     2521 tgatttctac cgggatcacc tacaaattct gataatgcat ctttccaggg cattttgtgc
     2581 ataaaaacag ggcgtcacgc cctgttttgc attacggtgg tgcaatacct gggaggaaac
     2641 aatgcaacaa aaaatgattc aatttagtgg cgatgtctca ctgccagcca tagggcaggg
     2701 aacatggtat atgggcgaag atgccagtca gcgcaaaaca gaagttgctg cactacgcgc
     2761 aggcattgaa ctcggtttaa ccctcattga taccgccgaa atgtatgccg atggcggtgc
     2821 cgaaaaggtg gttggggaag cattaaccgg tctgcgagag aaggtctttc tcgtctctaa
     2881 agtctatccg tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg
     2941 ccgtctcaat actgactatc tcgatctcta cttattacac tggtctggca gtttcgcttt
     3001 tgaagagact gttgctgcga tggaaaaatt gatcgcccag ggaaaaatcc gtcgctgggg
     3061 cgtttctaac cttggttatg ccgatatgca ggaactctgg cagctgccgg ggggaaatca
     3121 gtgtgccact aatcaggtgc tttaccatct cggttcacga gggattgagt acgatctact
     3181 cccctggtgc cagcaacagc agatgccggt gatggcttac agtccattag cccaggccgg
     3241 gcggttgcgc aatggactgt taaaaaacgc ggtagtcaac gaaattgcac atgtccaaca
     3301 acatgcagct gcgcttgagg tcgaactttc gtcagcggaa ttagctatgc tggataaggc
     3361 atatccggca ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat acgtgccgga
     3421 tgcatcgcac ccggcacaca acattaacgc aaattaacgt ttggcaacgc gaatggattg
     3481 cgccagatgt gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc
     3541 tacacaaaca aaggtcttgt agccatcgtc cggcatgtcg cccatgctaa ctgaaagcgc
     3601 cggtcccggg ttccagccga caacgttcag atgatgctgg tggcctacgg cgataatacg
     3661 attcagcgct tcatcattaa tcacgctgca atcttgcgga ttcagataca cgcgatcggt
     3721 acggtcaggg aaggtctgaa taccgtcggt cagtacatct tctttcgcgt cattcacttt
     3781 atcaatgaag cgatcgccca gcccactgac gcttacctta gcgatatcac ccacgttaaa
     3841 gtaggtatgc agggcagagg tggtttcaaa ttcgccatgt gattcaagat cgatttcaca
     3901 ggttttaccc acgcggaaat gcgctaacag cgtaaagtcg tgcggccaga attttttcgt
     3961 ctcttcgctt tgtgtcaatt caaaagtcag cgctacgcca tcagcatctt catgatgtga
     4021 tttcagcgtc cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt gttgtgccgc
     4081 cggaccaaac cacggccagc aaaccggtac gccaccgcga atagcgacgc catttttgaa
     4141 cggtgtgttg ttgctcaacc acagaacttc ttcttcaccc gcaggtttcc acgagagaag
     4201 gtgtgcgccc tgtaatgcaa aagaggcttt tacctgggga tgatcgacca caatgaggtc
     4261 cagttcatcc agtttacgac gggagaggac aggggagatt tgttcgatga ccggaagggc
     4321 aaaaattttc ttaatcatga cgcagtcctt taacttcatt ttatcaggta aaaaaaagag
     4381 cgaccgaagt cgctcttttt agatcacagt gtcatctcaa cttatttgga gatgtgagcg
     4441 atcaggtcca gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc
     4501 acgaagttgt cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg
     4561 ccgttgaaat cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg
     4621 ccttcagcag cagctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga
     4681 cgaacggtca ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc
     4741 agtttgccat tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac
     4801 gggatgatgt tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca
     4861 acggttttct gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg
     4921 aagttatcgt tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg
     4981 gaaacgatgt cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg
     5041 ttgtctttag acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga
     5101 gcagtttcgt cagtcaggaa cagaccagtt gcttcagcga caacgtcaac acccacttcg
     5161 tcccatttca ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg
     5221 atcagatgac cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat
     5281 ttcagcatgt atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg
     5341 tcagaacgtt tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata
     5401 cctactttga tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta
     5461 aaattacaaa aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa
     5521 tcctgtgcct aagcattacg cgactgactc gcctcacttt tcctttgggc ttgagaccac
     5581 atggggtcgg cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg
     5641 atttaggtgc agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt
     5701 aatgttttgt tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagtaaat
     5761 ggcgaataaa ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt
     5821 gacgcagaat catgggacag aaccgccatt tacgggtcgt ttactgcata acaagcgtga
     5881 cggcgtatat cactgtttga tctgcgatgc cccgctgttt cattccgaaa ccaagtatga
     5941 ttccggctgt ggctggccca gtttctacga accggtgagt gaagaatcca ttcgttatat
     6001 caaagacttg tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca
     6061 tctggggcat gtctttcccg acgggccgca accaacaggc gaacgttatt gtgttaactc
     6121 tgcttcttta cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga
     6181 ttcagcaact tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc
     6241 atgacgcctg aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcctgat
     6301 ggcgttgcgt tgaccgagga acaaaaagag aattgcctgc aactggtgat gctgtggcaa
     6361 gcccgccata ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg
     6421 aagagcaaac agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt
     6481 aagtaagtgg ttcatcccct ttttgccgcg gctatgaagg ggatagccaa caatgacccc
     6541 acgactcgtc tcaattatct cgacccgacc tggctaagtc ccagtccgat ctcttgcagt
     6601 cgcgttcacg cctgaatgac ttctcctgaa cttaagtctc agacctattt ggccggtaat
     6661 ccctctcgaa tttgctcttt ttgcgtcccg ccttccacca acctgtaaca gacccagcca
     6721 ttttctgcgt aattgtgatc tgagttgtaa aatgcaggtc attatctgtg ttgtgcagat
     6781 ttaatattga cctcaaaaat catgtgggta agtatgatgg ccatctaagc aaaacaaaac
     6841 acagaggatg acgcaatgaa agcactggct cggtttggca aggcctttgg cggctacaag
     6901 atgattcatg tcccacaacc catttgtggc ccggaagacg tagtgattga aattaaagcc
     6961 gcggcaatct gcggcgcaga catgaagcac tacaatgtcg atagcggttc tgatgagttt
     7021 aactctatcc gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa
     7081 gactggaaag tggggcaacg cgtcgtgtcg gataacagcg gtcacgtttg cggcgtttgt
     7141 ccggcctgtg aacagggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat
     7201 aacaatacct ggggcggtgg tttttccaaa tattgtctgg ttcctggtga aattctcaaa
     7261 attcatcgtc atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta
     7321 cttgacccta tctgtaatgc ctataaatcc atcgcgcagc aatcgaaatt ccttcctggt
     7381 caggatgttg tcgtcattgg cactggccca ctcgggctgt tttccgtaca aatggcgcgg
     7441 attatggggg cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc
     7501 ccggttgcaa aagaactggg ggcgacggca gtagtaaatg gttctaccga agatgtggtg
     7561 gcgcgctgcc agcaaatttg tggcaaagat aatctgggac tggtgattga atgctccggt
     7621 gccaatatcg cactgaaaca agccatcgaa atgctccgcc cgaatgggga agtggtacgc
     7681 gttggaatgg gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaataaa
     7741 agcatcattg ggcatatggc ctatgactcc acctcatggc gtaacgctat ccggctatta
     7801 gccagcggcg ctatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg
     7861 cgcgaagggt ttgatgcgat ggtcgataaa accgcaatca aagtgatcat gacttacgac
     7921 tttgatgaat aactactctc ctgaaggaac gttatcgcat ggaacagata acaaaaccgc
     7981 attgtggtgc acggctggat cgcttaccgg attgccgctg gcattcgtca atgtttgcca
     8041 tcgtcgcgtt tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc
     8101 agctgaaagc gttgggctgg acagataatt ccactacagc cacattctca gcaatcacga
     8161 ccgccggaat gtttctcggt gcgctggtgg gcggcatcat tggcgacaaa accggtcgca
     8221 gaaatgcgtt catcctctat gaggccattc atattgcctc gatggttgtc ggtgctttct
     8281 caacgaatat ggattttctt attgcctgcc gttttgtgat ggacgttggg ctgggagctt
     8341 tactggttac actgtttgct ggtttcaccg aatatatgcc cggtagaaat cgcggaacgt
     8401 ggtccagtcg ggtttccttt attggcaact ggtcatatcc gctctgctca ctgattgcga
     8461 tgggactcac gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttattcctg
     8521 caatattgtc gcttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct
     8581 ggctggaatc gcgcggacgg tatcaggaag ccgagaaagt catgcggagt atagaagaag
     8641 gcgtcatacg ccagacgggt aaacctttgc cgcctgtggt tattgctgat gacggtaaag
     8701 cgccacaagc ggtgccgtat tcagccttac tgacaggagt attactgaaa cgcgtgatat
     8761 taggctcttg tgtgctgatt accatgaacg ttgtgcagta cacactaatt aactggttgc
     8821 caacaatatt catgacccag gggattaatt taaaagactc gattgtttta aataccatga
     8881 gtatgtttgg cgcgccattt ggtattttta ttgccatgct ggtgatggat aaaattccgc
     8941 gtaaaacaat gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctattcac
     9001 tgcaaaccag tatgttgctc ataacgctga ttggtttctt cctgattact ttcgtctata
     9061 tgtacgtttg ctatgcctcg gcagtgtatg tccctgaaat ctggccgaca gaggccaaac
     9121 tccgtggctc cggtctggcg aatgcggtag ggcgaatcag tggtattgcc gcaccttatg
     9181 ccgttgcagt gctgctcagt agttatggtg taacgggagt ctttattctt ctgggggctg
     9241 tttcaaatat tgtcgcaatt gctatcgcca ccattggaat tgaaaccaaa ggtgtctccg
     9301 ttgaaagttt aagtattgat gcagtagcca ataaataata cgggtggatt caaaatgaaa
     9361 aattcaaaag caatattgca ggtgccgggc acaatgaaaa ttatttcagc agaaatacca
     9421 gtgcctaaag aagatgaagt tttgattaaa gtagaatatg tcggtatttg tggttcagat
     9481 gtacatggtt ttgaatcagg cccgtttatt ccgcctaaag acccaaatca agaaattggc
     9541 ctgggtcatg aatgcgccgg gacggttgtg gctgtgggaa gccgcgtgcg caaatttaaa
     9601 ccgggggatc gggtaaatat cgaacctggc gttccttgcg gtcactgtcg ttactgtctg
     9661 gaaggcaaat ataacatctg cccggacgtt gattttatgg cgacacaacc caactaccgc
     9721 ggcgcattaa cgcactatct gtgtcatccg gagagcttta cttacaaact gcccgacaat
     9781 atggacacga tggaaggggc gctggtggag cctgccgcag tcgggatgca tgccgcgatg
     9841 ctggcagatg ttaaaccggg taagaagata attattctgg gagcaggttg tattggtttg
     9901 atgacgttgc aagcgtgcaa atgcctgggc gcaacggaaa ttgccgtcgt tgatgtactg
     9961 gaaaaacgtc tggcaatggg ggaacagcct ggtgcgacag tggttattaa tggcgcaaaa
    10021 gaggacacta ttgcacgctg tcagcaattt accgaagaca tgggcgcaga tattgttttc
    10081 gaaacagcgg gttctgcggt caccgttaaa caggcacctt taatctggta atgcgcggcg
    10141 gtaaaattat gattgttggt actgtacccg gcgattcggc aatcaatttc ctcaaaatca
    10201 atcgcgaagt cactatccag acggtattcc gctatgccaa tcgttatccg gtcacgattg
    10261 aagctatttc ttcagggcga ttcgatgtga aatcgatggt gacgcatatt tacgattatc
    10321 gggatgtaca acaggcattt gaagagtcag ttaacaacaa acgcgacatt attaaaggcg
    10381 ttattaaaat tagcgattaa ttaatacggg agaacagaac atgctcgcag atatcagata
    10441 ttgggaaaac gatgccacaa ataaatatta tgcaattgcc cattttaacg tatggaatgc
    10501 agaaatgttg atgggcgtta aagacgcagc cgaagaagcg aaatccccgg ttattatttc
    10561 ttttagtaca ggttttgtcg gtaacacctc atttgaagat ttctctcaca tgatggtatc
    10621 aatggcacaa aaagcaacgg tgccggtaat aactcattgg gatcatggtc ggagtattga
    10681 gattattcat aacgcctgga ctcatggtat gaattcatta atgcgtgatg cttctgcatt
    10741 tgatttcgaa gaaaatattc gtttaaccaa agaggctgtt gatttctttc atccgctggg
    10801 tattccggta gaggcggaat tagggcatgt cggtaatgaa accgtttatg aggaagcgct
    10861 ggcgggctat cactataccg atcctgacca ggcggctgaa tttgttgaac gtacgggctg
    10921 tgactcattg gctgtcgcca tcggcaacca gcatggggtt tatacgtcag agccgcaatt
    10981 gaactttgag gtcgtcaaac gcgtacgcga tgctgtttct gttccgctgg ttttgcatgg
    11041 cgcatcgggg atcagtgatg ccgacattaa aactgcaatt tcgttgggta ttgcgaaaat
    11101 caacattcat acggagctct gtcaggccgc aatggtagca gtgaaagaaa accaggatca
    11161 gcctttcctg catttagaac gtgaagtacg taaagctgta aaagaacggg cattggaaaa
    11221 aattgaactg ttcggttctg atggcaaagc ggaataatga tatggataat ctcgacgtta
    11281 tttgtatagg tgccgctatt gttgatattc cattacaacc ggtcagtaaa aatatctttg
    11341 atgtagattc ttatcctctt gaaagaatcg caatgagcac cgggggcgat gcaataaatg
    11401 aagcaacaat tatttctcgt ctggggcatc gtaccgcatt aatgagccgc attggtaaag
    11461 atgcctcagg gcaatttatt ctcgaccatt gtcgcaaaga gaatattgat attcaaagcc
    11521 tgaaacagga cgtcagcata gatacatcta ttaacgttgg actggtgacg gaggatggcg
    11581 agcggacgtt tgtcacgaac cgtaacggca gtttgtggaa attaaatatt gacgatgttg
    11641 attttgcgcg gtttcctcag gcgaaattat tatcactggc cagtattttc aacagtcctc
    11701 tattggatgg taaagcgcta acagaaattt ttacgcaagc gaaagcccgg cagatgatca
    11761 tttgtgccga tatgatcaaa ccgcggttga atgaaacgct ggatgatatt tgcgaagcat
    11821 tgagttatgt cgattatctg tttccaaatt ttgccgagga aaaattactc acagggaaag
    11881 agacactgga tgaaattgct gactgctttc ttgcgtgcgg cgtaaaaacg gtggtgatta
    11941 aaacgggtaa agacggctgc tttatcaagc gtggtgacat gacgatgaag gtgccggcgg
    12001 tcgcaggaat aaccgccatc gacaccattg gcgcgggcga taactttgct tcaggtttta
    12061 ttgcggcact gttagaaggc aaaaatctgc gtgaatgcgc acgctttgcc aatgcaacgg
    12121 cggctatctc ggttctaagc gtcggtgcca ccaccggcgt aaaaaacaga aagctggtcg
    12181 aacaattgct ggaagaatac gaaggataat gaaggcaaat gaaaaagata cctttaggca
    12241 cgacggatat aacgctttcg cgaatggggt tggggacatg ggccattggc ggcggtcctg
    12301 catggaatgg cgatctcgat cggcaaatat gtattgatac gattcttgaa gcccatcgtt
    12361 gtggcattaa tctgattgat actgcgctag gatataactt tggcaatagt gaagttatcg
    12421 tcggtcaggc gttaaaaaaa ctgccccgtg aacaggttgt agtagaaacc aaatgcggca
    12481 ttgtctggga acgaaaagga agtttattca acaaagttgg cgatcggcag ttgtataaaa
    12541 acctttcccc ggaatctatc cgcgaagagg tagaagccag cttgcaacgt ctgggtattg
    12601 attacatcga tatctatatg acgcactggc agtcggtgcc gccatttttt acgccgatag
    12661 ctgaaactgt cgcagtgctt aatgagttaa aggctgaagg gaaaattcgc gctataggcg
    12721 ctgctaacgt cgatgctgac catatccgcg agtatctgca atatggtgaa ctggatatta
    12781 ttcaggcgaa atacagtatt ctcgaccggg caatggaaaa cgaactgctg ccgctatgtc
    12841 gtgataatgg cattgtggtt caggtttatt ccccgctaga gcagggattg ttgaccggca
    12901 ccatcactcg tgattacgtt ccgggcggcg ctcgggcaaa taaagtctgg ttccagcgtg
    12961 aaaacatgct gaaagtgatt gatatgcttg aacagtggca gccactttgt gctcgttatc
    13021 agtgcacaat tcccactctg gcactggcgt ggatattaaa acagagtgat ttaatctcca
    13081 ttcttagtgg ggctactgca ccggaacagg tacgcgaaaa tgtcgcggca ctgaatatca
    13141 acttatcgga tgcagacgca acattgatga gggaaatggc agaggccctg gagcgttaaa
    13201 tactcaacac gattcacctc tgtttctgtg aaaactgtga tgaaaatctg tgattcaaat
    13261 aggttatttt gaaagtaaac atcggttatg cgataatcgc gctaatgtga tgtaaaaaga
    13321 tacaggggtg taatcgtggc ggcaaaagac aggattcagg caattaagca aatggttgcc
    13381 aacgataaaa aggtgacagt ctcaaatttg agtgggattt ttcaggtaac cgaagaaacc
    13441 attcgccgcg atcttgagaa gctggaagat gaaggctttt tgaccagaac ctatggtggt
    13501 gctgttttaa atacagcggt gttgacggag aatatccatt tttataagcg cgcttcatcg
    13561 ttttatgaag agaagctgct tattgcacgc aaggcactac cctttatcga caataaaacc
    13621 accatggcag ccgattccag tagtaccgta atggaattgc tcaaattgtt acaggaccgt
    13681 agtgacctga cgttgctaac caactcggcg gaagcgattc atgttctggc tcagtcagaa
    13741 attaaagtcg tttcaacggg tggggaacta aacaaaaata cactttccct gcaaggaaga
    13801 attactaaag agatcatcag ccgctaccat gtcgatatca tggtaatgag ttgtaaaggt
    13861 cttgatatta acagcggcgc gctggactct aatgaagcgg aagctgaaat caaaaagaca
    13921 atgatccgtc aggcgacaga agttgcgtta ttggttgatc actctaagtt tgatcgtaaa
    13981 gcttttgtcc agttagctga ctttagtcat attaattata taataactga taaatcaccg
    14041 ggtgcagagt ggatagcatt ttgcaaagac aataatatcc agctggtgtg gtaatttaag
    14101 cacacgttgt caatattatt tgttcctgcg actggaaata aaatgatgat aaagatgcgg
    14161 caagtgaatt tgccgcaatt gctacctttt atagcaaatc cgaggcaccc aatggaacaa
    14221 tatgatcaaa ttggcgcaag actggaccgc ttgcctttgg cccggtttca ttatcgtata
    14281 tttggtataa taagctttag tctgttatta acggggtttt tgagttactc aggtaatgtc
    14341 gtcttagcaa agctggtaag caatggatgg tcaaataatt tcctctatgc cgcctttacc
    14401 tcggcattaa tgtttggtta tttcatcggc tcacttactg gtgggtttat tggtgactac
    14461 tttgggcggc gcagggcgtt tcgcataaat cttctcatcg tcggtattgc tgcaacaggg
    14521 gccgcttttg tccctgatat gtactggctc attttctttc gcttcctgat gggaacagga
    14581 atgggggcgc tgattatggt tggctatgcc tcatttacgg agtttatccc cgcgacggtg
    14641 cgtggaaaat ggtccgcgcg gctctcattt gttggtaact ggtcgcccat gctgtctgcg
    14701 gcgataggcg tggtggttat cgcttttttt agttggcgaa taatgtttct tctgggtggt
    14761 attggcatac tgttagcctg gtttctctca ggtaaatact ttatcgagtc gccacgatgg
    14821 ctggaaggga aagggcaaat cgccggtgca gaaagccaac ttcgtgaagt agagcagcaa
    14881 attgaaaggg agaagagtat tcgtttaccc cagcttactt tgaaccagag caacagcaag
    14941 gttaaggtaa tcaagggtac cttcaggctc ctgtttaaag gggaaatgtt acgacgtaca
    15001 ttagttgcga taactgtttt aattgcaatg aacatttcgc tttataccat taccgtatgg
    15061 ataccgacca tatttgttaa ctccggcatt gatgtcgata aatcaatatt aatgaccgct
    15121 gttattatga ttggtgctcc ggtaggaata tttattgcgg cattaattat tgatcatttt
    15181 cctcgtcgat tatttggctc cgccttactt attattattg ccgtgttagg ctatatctat
    15241 tcaagtcaga ctacagagtg ggcgatttta atctatggtc tggtgatgat cttcttttta
    15301 tacatgtatg tttgcttcgc gtcggcggtt tatatcccgg agctttggcc aacgcattta
    15361 cgcctgcgcg gttcgggttt cgttaatgcc gtcggacgga tcgtcgcagt tttcacgccc
    15421 tatggcgttg cggcattatt aacacattat gggtcgatca cggtgtttat ggtgcttggt
    15481 gtcatgttag tgctctgtgc gctggttctc tccatttttg gcatcgaaac gcggaaggtg
    15541 tcgctggaag agatttctga ggtgaattaa atctttcgtt gatggcatca attgccagat
    15601 gtaccactga cgtgttttat ttggcctaca atgccagtac agaattgtag gccagcgtaa
    15661 aattacccct gcgtctcttc ccagtctgcc agcgtatata gcgttgcccc agctgctgac
    15721 atctccataa acgcgtgtgc actgtcctgg ggctggatat tcacgccacg acagccatcg
    15781 gtaatcacgt ttaccttata acctaactgt aacgcgtcca gcacggtaaa cttcacgcaa
    15841 tagtcagtag ccaggcccat aacgatcaat tcatcgattt catgatcgcg caaccagtca
    15901 tcaagcgcgg ttttctgccg acgtccgtta tcaaaaaagg cactgtaact gtcaactaaa
    15961 ggattttcgc ctttatggaa caccgctgcg atctcttttt gattcagtaa cggatgtaat
    16021 tgtgcgcctt cactgttctg cacacagtgt tcaggccaga aagtttgccg caggccgccg
    16081 agttggcctg gcgtataagg ctctacgccg tgctggctgg caaagctgcc gtgattcgcc
    16141 ggatgccagt cctggctggc gataaccgct tctccgcgcg actggcacca gtcaataagg
    16201 cggttagcaa cctccaccgt actgtcacct tccggtacgg cgagcgcgcc accagcacag
    16261 aaatcatttt gtaaatcgac cagtaacagg gcgcgatggg gcattgcagt ctccttaatc
    16321 atccggcgtc agttcgccgc gcaggttttg gctcatggcc ttgcgaatgg tttcagtatc
    16381 cagttcctgg ctcagcaggt aatgcagttt ggttagcgtg gcttctacag tcatatctgc
    16441 accgtcaata acgccggcat gggcgagggc gttaccggtg gcataaccac tcatattcac
    16501 tttaccggac atacattgtg tcaggttgac caccacaata ccgcgatcgc tggcttcttg
    16561 taattcctgc aggaaggctt tgttttgtgg cgcattaccc acgccatagg agcgcagaat
    16621 caatgctttc accggttggc gcagaaaatt gcgcacgacg tcagcagaaa tccctggata
    16681 aatcgtcacc acgccaattg gttgtggcgt gattggatga acgattaatt ccccttcacc
    16741 gtgcggggcg ggtggcgtat tcaaacgacg aatatggata ccagcttcca gtaacggagg
    16801 aaggtttgga gaggcaaacg catcaaaacc atcggcatgg gctttggtag tgcggttgcc
    16861 gcgatacaat cggttattga aaaagagcgt tacttcgttg atcggataat tcgccgcaac
    16921 gtacaacgca ttcagcagat taatttgtcc gtcagagcgt aactcagcca gcgggatttg
    16981 tgaccctgtc acaatgaccg gtttaccgag attctcgagc atgaacgaca gcgcagaggc
    17041 ggtatacgcc atcgtgtcgg taccgtgtag aatgacaaaa ccatcatagt cgtcatagtg
    17101 cgctttaata tcttcagcaa tatgctgcca gtcttccggc gtcatatcag aagaatccat
    17161 caacggcgta tattcatgaa tggtgaaatc tggcatctcc gggcgatgga attccggcat
    17221 cagcgccagt tggcgttgta gatgacctga caccggaata taaccctgct cggaacgctg
    17281 catcccgatg gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca tgatattgat
    17341 atgaatgata tcgaaagagc aaaagtatat acgtgaggca ggaggagcgc acctgatact
    17401 atgcggataa ggaaatatgg ggcgagacag cgggaagagg attcgtcgag gtaaaaaata
    17461 atgtggccga cgatctgtcg gccactcagt acaagactta acgcacgttg gcgcaggtca
    17521 ggcaaaacgc ataacggttt tgtgggtcgt taaacgcggc cagcttgtcg ctttcacttt
    17581 taacggtaga ggctaccgag gcaagcggtg caggtaacat ggcctggaac gcatctggca
    17641 acattgcccg gacagaacca gacatgttgt ccatcacttt gtcgaagaag gtcggttcat
    17701 caacgtagta ttccagatgc cactgtttca cttttgcgag ctctgctgct ttggcaaccg
    17761 catcatcgaa atccccgaga ctatcgacca gcccgttagc ttttgcatcc tgaccggtcc
    17821 agacgtggcc ctgggcgatt ttatcaattt gctccggcgt cgaatgacgc gcatcagcaa
    17881 ccagcgtgat aaagcgttta tagccattct caatgcttaa ttgcatcatc tgctgcgctt
    17941 ccggcggcag tgccctggtg atagaaacat ccgccagcgg tgaagttgag acaccatcag
    18001 tatgaacacc aatcgaatcc agactatttt ctacggtggt gatcacgccg aagataccga
    18061 tagaaccggt cagggtgctg gggttagcca caatgtaatt agctggcgtg gaaatccagt
    18121 aaccaccaga tgccgccatg ccgcccatcg atacaaccac agacttaccc gctgcccggg
    18181 ctgctgccag ttcagcgcga atcacttcag acgcggtaac gctgccgcct gggctattaa
    18241 cacgcaggac aatcgctttc actttcgggt caaggcgagc atcgcggatt tgtgccgcag
    18301 tggtatcacc gccaacattc ccctgagttt cctcgccatc cataattgcg ccattagcga
    18361 agacgacacc gatgctgtca ccggtatctg ccggcgtttt caatgcgtaa tcgtaataac
    18421 tgatggcgcg ataattttta tcagtcttac tccagccgaa ctctttggtc agagcttttt
    18481 cgatttcggc actcgatgcc agtgcatcga ccagcttgtt ttccagtgca tatttcgcgg
    18541 tatcgccacc ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg aatacctgct
    18601 gagcagggat ctgccggtta gcggcaacag tattcagata gttttgccac agctcaccaa
    18661 tccagcggct gtcagcttcg cgggctgccg gtgacatatc atcacgaata aacggttcaa
    18721 cggcagattt atacgtaccc acgcggaaca catgggtgga aactttcagc ttatccagca
    18781 acgatttgta gtacagaccg ttggtagcaa aaccgtgcag atcaaccacg ccttgcggtg
    18841 acagccaaat tttattggcg aaactggcga gataatattg cccctggctg tagttctcgc
    18901 caacggcata aaccggtttc ccgctgtcac gaaactcttt cagggctttg ccgatgtact
    18961 gcatagacgg ctggtcgccg cctgcgaagt ttttcagatc catcacgata ccggtgatat
    19021 tgcggtcgtc cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag ttttcctgca
    19081 gacgatcgga actggcacca agcagctggc ggcttaattt actaaaccgc tgagaactgt
    19141 ctggtttatc gacgatcaca ccagaaatgt ccagcagcag tgcgccacga ctggccgttt
    19201 ctttcgaatc accaccactg acctgcatcc agatccccac accaaccagt acgaggaaaa
    19261 taaagaacag gttaagtacc atttcacgga cgaaattcag cagacgccac gtccatttaa
    19321 aaaatccggc aataaatcgc caaagggttc gcatgtattc tcccaactta aggtcacacc
    19381 tgtcacaggc gcaatagatg tggttatcgt aatgacccaa ccggcacttg tcagcaggaa
    19441 ttgcggactg tgctgtaaca aaatccactc tcgtgttaat tttgtgaata aatatcacga
    19501 caggagttaa tcaaatggat gcactcgaac tattgatcaa tcgccgtagc gcctcccgcc
    19561 tggctgaacc cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg ggtatgcgtg
    19621 cgccggacca taagtccatg caaccgtggc atttttttgt gattgaaggg gaagggtgcg
    19681 agcgtttcag cgccgtactg gaacaggggg cgattgctgc cggtagtgat gataaagcta
    19741 tcgacaaagc ccgtaatgcg ccgttccgcg caccgctcat catcacggtg gtggcgaaat
    19801 gcaaagaaaa tcataaagtc ccgcgatggg aacaggaaat gtctgccgga tgcgcggtca
    19861 tggcgatgca aatggcagcc gttgcccagg gatttggcgg catctggcgc agtggcgcat
    19921 taactgaaag tccggtagtg cgtgaagcat tcggttgccg tgagcaggat aaaattgtcg
    19981 gttttctcta cctcggcacg ccgcagttga aagcatctac gtcgattaac gtcccggacc
    20041 cgacgccgtt tgtaacttat ttctgataat tgtcgcgaaa ctgtctggat tgtgagcaaa
    20101 ggcgcaggaa ttcagacact ctcacttatc acttcacgga atgagggtta ccatacccgg
    20161 attgcattga caggagatgt ccatgagcga gaactcgatt cgtttgaccc aatacagcca
    20221 cggagctggt tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc tgcacagtga
    20281 gcaggcgaag tttgttgatc cgaatttgct tgtgggtaat gaaacccgcg acgatgcggc
    20341 ggtgtacgat ctgggcaatg gcaccagcgt tatcagtacc accgacttct ttatgccgat
    20401 cgttgataat cctttcgatt ttggccgcat tgcggcgact aacgccatca gcgatatctt
    20461 cgcgatgggg ggcaaaccga ttatggcgat tgcgatcctc ggctggccga ttaacaaact
    20521 ttccccggaa attgcccgcg aagtaaccga aggtggacgc tatgcatgcc gtcaggcggg
    20581 tattgcgctg gctggcggtc actccatcga tgcgccggag ccgatttttg gtctggcggt
    20641 aacggggatc gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag ccggatgcaa
    20701 actgttcctg acgaaaccgc tggggatcgg cgttcttacc acggctgaga aaaaatcact
    20761 gttgaaacca gaacatcagg gactggcgac ggaagtgatg tgccggatga acatcgcagg
    20821 cgcgtccttt gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg gctttggtct
    20881 gctgggccac ttgagcgaaa tgtgtcaggg ggctggtgtg caggcacgcg tcgactatga
    20941 agcgatcccg aaactccccg gtgttgaaga gtacattaag ttgggcgcag tacctggcgg
    21001 cactgaacgt aactttgcca gctacggtca tctgatgggt gaaatgccgc gtgaagtgcg
    21061 cgatctgctg tgcgatccgc aaacttctgg cggtttgctg ctggcggtca tgccggaagc
    21121 agaaaatgag gttaaaacta cagccgccga gtttggcatt gaactgacgg cgattggcga
    21181 actggtgcca gcgcgcggcg gtcgtgccat ggttgagatc cgttaattta atgcggttgt
    21241 ttattgccga aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc aaaccgcacc
    21301 ggaaaggcga tggctttatc gagtgcggta atggtcaggt ggtgacttgg tgtatcggtc
    21361 acctgcttga gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc tggaatcttg
    21421 cggatttgcc gattgtcccg gaaaagtggc aattacagcc acgaccctcc gtgaccaaac
    21481 aacttaacgt catcaaacgc ttcctgcatg aagccagcga aatcgttcac gccggggacc
    21541 cggatcgtga agggcaattg ctggtggatg aagtgctgga ctatctgcaa ctggcaccgg
    21601 aaaagcgcca gcaggtgcag cgttgcttga taaacgacct gaacccgcag gcggttgagc
    21661 gggcgatcga ccgtcttcgt tccaacagtg agtttgtgcc attgtgcgtt tctgcgctgg
    21721 cgcgagcgcg tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat accattctcg
    21781 gtcgcaatgc cggttatcag ggcgtacttt ccgtgggacg cgtgcagaca cccgtgctcg
    21841 ggctggtggt gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac ttctttgaag
    21901 tcaaagcaca tatcgtgaca cctgccgatg agcggtttac cgctatctgg caaccgagcg
    21961 aagcgtgtga accgtaccag gatgaagaag ggcgcttgtt acatcgtcca ctggcggagc
    22021 atgtggttaa ccgcattagt ggtcaaccgg ctattgtcac cagctataac gataaacggg
    22081 aatcagaatc cgcgccgctg ccgttttcgc tttcggcgtt gcagattgaa gcggcaaaac
    22141 gcttcggtct gagcgcgcag aacgtgcttg atatctgcca gaagctgtac gaaacgcaca
    22201 agctaatcac ttatccgcgt tccgattgtc gctatttgcc agaagaacat tttgccggac
    22261 gccacgcggt gatgaatgcc atcagcgttc atgcaccgga tctgttgccg cagccagtgg
    22321 tagatccaga tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg caccacgcca
    22381 tcatcccgac cgcacggagt tctgcgatca acctgacgga gaacgaagcg aaggtctata
    22441 acctgattgc ccgtcaatac ctgatgcagt tctgcccgga tgcggtgttc cgcaagtgtg
    22501 ttatcgaact ggacattgcc aaaggcaaat ttgtcgctaa agcccgtttt cttgctgaag
    22561 caggctggcg cacgctgtta ggcagcaaag agcgcgatga agaaaacgac ggcacgccat
    22621 tgcctgtggt ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg gtagagcggc
    22681 aaacccagcc gccgcgccat tttaccgatg caacactgct ttcggcgatg accgggatcg
    22741 cgcgctttgt gcaggacaaa gatctgaaaa aggtccttcg tgcgaccgat ggtctgggga
    22801 cagaagcaac ccgtgccggg attattgaac tgctgttcaa gcgtggtttt ctgaccaaaa
    22861 aagggcgcta tatccactcc accgacgccg gaaaagcgtt attccattcg ctgccagaaa
    22921 tggcgacgcg accggacatg accgcgcact gggaatcggt gctgacgcaa atcagcgaaa
    22981 agcagtgtcg ctatcaggac tttatgcagc cgctggtggg gacgctatat cagcttattg
    23041 atcaagccaa acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg ggcagtggtg
    23101 gcagtgctga taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa agtccgccag
    23161 cagatgaagc cggaagcggg gcgatagcgt aagcgagcga atctttcgtg ctattcgagt
    23221 catattctga aatatccagc ggatcaagaa aattcgttgg atattttttt gcatggataa
    23281 aattatcgcc tctaaagtat gtaataacag ggaatgtgat gaaaaaagtt ttattgcaaa
    23341 accatcctgg gagcgagaag tattctttta atggctgggg aatatttaat agtaattttg
    23401 aacgggtgat taaagaaaat aaggccatgc tgctttgtaa gtgggggttt tatttaacat
    23461 gtgttgtcgc tgtaatgttt gtattcgcag cgataacatc caacggtttg aatgaaagag
    23521 gcctgattac cgcgggatgc tcttttcttt atctattaat tatgatgggg cttattgttc
    23581 gggccggttt taaagcaaaa aaagaacaac tgcattatta tcaggctaaa ggtattgagc
    23641 cgctcagtat cgagaagtta caggcgctac aattgatcgc accttatcga ttctatcata
    23701 agcaatggtc tgaaacgcta gagttctggc cgcgaaagcc tgaacctggc aaagatacct
    23761 tccaatatca tgtgcttcct tttgactcga tcgatatcat cagtaaaaga cgcgagtctt
    23821 tagagaatca atggggtatc gaagatagcg aaagttattg tgccttaatg gagcattttc
    23881 tttctggcga ccatggagcc aataccttta aagcaaacat ggaggaagcc ccagagcagg
    23941 ttatcgcctt gttgaataaa tttgctgttt ttccctcaga ctatatctct gattgcgcta
    24001 ataatcgctc cggtaaatcc tcggcgaagc taatatgggc ggcggaatta tcatggatga
    24061 tctcgatatc aagcacagct tttcaaaacg ggacaattga agaagaactg gcctggcatt
    24121 atataatgct tgcttctcga aaggcgcacg agttgttcga aagcgaagaa gattatcaaa
    24181 aaaatagtct aatgggattt ctttactggc atatctgctg ctatcgcaga aagttaacgg
    24241 atgctgaact tgaagcgtgt tatcgttacg acaagcagtt ttgggagcac tacagtaaga
    24301 aatgccgttg gcccataaga aatgttccct ggggagcatc atccgttaaa tactcataaa
    24361 cgcctgaaat tttgccgggg gcgctgcgct tgaccggcct acattagagc gtaactgcaa
    24421 tgaattgtgc ccatctgtag gcctgataag cgtagcgcca tcaggcattt acaacttaaa
    24481 tcacaccctg cgccagcatc gcatcggcaa ccttcacaaa accggcaatg ttcgcgccct
    24541 gcacgtagtt ggtttgctca ccttcaccac catgctcaac acaggcatgg tggatatcca
    24601 gcatgatgtg atgcaaacgt gcgtcaactt tctcggcttt ccagcccagg cgcgcagcgt
    24661 tttgtgccat ttccaggccc gacgttgcta caccgccagc attagccgct ttacccggtg
    24721 caaacagtac gcctgcctgc tggaacagtt cagtcgcttc gatggtggtc ggcatatttg
    24781 ccccttcagc gacggcttta acgccattgg cgataagctg atgcgcggcg tcaacatcca
    24841 gttcgttctg ggtggcgcaa ggcagggcga tatcaaccgg tacagaccac ggctgttggc
    24901 cttcgagata gaccagacca aattctttgg cgtaatctgc cactcgacca tcgcggctgg
    24961 ctttgatttc gataagacgt gccagtttct ctttcgtgaa tccgctttca tcaactacag
    25021 tgccgctgga gtctgacgca gtgatcacac gagcaccaaa ttccatcgct ttttcgatag
    25081 cgtactgggc gacgttgccg gagccagaaa cggaaacgct catcccttca aaacccatac
    25141 cgtggcgttt taacattgct tctgtgaaat aaaccagacc gtagccggta gcttgcgaat
    25201 aagactaccg ccaaatgaaa ggcccttacc ggtgaagacg caggcggtat tgttggagag
    25261 ctttttcatc atccccgcca taaagccgac ttcacgacca ccaacgccga tatcacctgc
    25321 cggaacgtcg gtatccgcgc ccaggtggcg atacagttca gtcatcagcg tctggcaaaa
    25381 acgcatcact ttaccttcgc tttttccttt cggatcgaaa tcgctgccgc ctttaccacc
    25441 gcccatcggc agggtagtca gggcattttt gaaggtttgt tcaaagccga ggaatttgag
    25501 aatggaaagg ttaactgacg gatggaagcg cataccgcct ttgtacgggc cgatggcaga
    25561 gctgaactgc acacgccatg cacggttgac ctgtatctgg ttgcgatcat caacccatac
    25621 cacgcgaaac tggatcacgc gctccggttc aaccagacgc tccagtaatg acatctggcg
    25681 atattttgga ttttgttcaa gaaaaggcca gagtgtggtc attacttcac gaacggcttg
    25741 cgcgaactcg gtttgattcg ggtcgcgctt ttggacatgg ttgaggaatg actccagaga
    25801 atatgtctga tccatagata taaaaacctt atatattaat acgattgtgc ttatgttgtt
    25861 tatgtcatgt gcttttgcag ttttcgacta taccatcaag aatgtgacgg taagcaagaa
    25921 aaaaatgacc caggaaagca aattaataac gagagtaatc tcataaccaa atgcgataac
    25981 tcagacgcta aagttttgcc acccggctac cgttatcagt agcagagaca ataacgttaa
    26041 ggagttccct gtgagtcgag cattgttcgc cgtagtatta gcatttccat taattgcact
    26101 ggctaacccg cattatcgtc cggacgtgga ggtaaacgta ccgccagaag tgttcagttc
    26161 aggcgggcag tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc aaaattattc
    26221 tgaaggggcg gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg atgacaaaac
    26281 ccttagcact aatccgctgc tctggcggcg cgtaaagcca taaacgcctc taataatgga
    26341 atgtcagcag gggccagcgg atattgcagc gcctcttcag gtgagcacca gaccagcgcc
    26401 tgatgttcat gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc atgaagatgg
    26461 ataatccggc ccgaaacttc tcgctgatgg ctggcaacat actcacccac agttgcttcg
    26521 atgtccaatt cttcgtttaa ctcacgcacc agcacctgct gctggctttc atccagctcg
    26581 actttacctc cggcaaactc ccataatccc gcctgatcgc tctgggcggg gcgttgcgcg
    26641 agtaaaattt tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat tttcataaat
    26701 tacacacggg cagataagcg aaaaatgcca taatatccgc actgttgctc aatcagaaag
    26761 gctttatatt taaggagtgc ctgtgctaga ccgccatctt catccccgga ttaaaccgtt
    26821 gttgcatcag tgcgtgcggg ttcttgataa accgggcatt acgccagatg gtttaacatt
    26881 agtgggattt gccatcggcg tgctggctct gccgtttctg gcgctgggct ggtatctggc
    26941 ggcgctagtc gtcattttgt tgaacaggct gcttgatggt ctggacggtg cgctggcgcg
    27001 gcgcagaggg cttaccgacg cgggcggctt tcttgatatt tctctcgatt ttctctttta
    27061 cgcgctggtg ccgtttggct ttattcttgc tgcaccggag caaaatgcgc tggcaggcgg
    27121 ctggttgttg tttgcgttta tcggcacggg cagcagtttt ctcacctttg ctgcactggc
    27181 agcaaaacat cagattgata accccggtta tgcacataag tcgttttact atctgggggg
    27241 attaaccgaa ggcacagaaa cgatcttact gtttgtgctg ggatgtttat tccctgcatg
    27301 gtttgcgtgg tttgcgtgga tcttcggtgc gttgtgctgg atgacgacct ttacgcgggt
    27361 gtggagtggg tatctgacgc tgaagtcgct ccagcgtcag taattgcaat gttatttgct
    27421 actgcccggg ccgcgttcac cggttgcaac cggatttttt ggatcgctgc tccattcgta
    27481 ccagccgccg tcatacacgg aaacattctt ccaacccatg gcgcgtgcgt acataaaggt
    27541 ttcggacgcg cgccagccgg tgccgcagta gaatgaaact tgctgatctg gtttgatatt
    27601 ccatgctttc cacatagcgg taatatcatc ggcgctacgc atggtgccat ccgggttata
    27661 gaaatcttcc atatgcgtcg agtcgctacc agcgtgtccc caacgtgctc cggctatttc
    27721 accttttggt ttaatatagc tgtaaccgct ggtcgtaccg ataaattctg gccacgaacg
    27781 aatgctcacc agcgatgcat cctggcgatg cagcagtcca cgggcctgtt ccatatcgag
    27841 catcaactgc ggttgtgccg ggatcttcac gccgaaatcc ggttccgctt tcactttcgg
    27901 tggcgttcct cgctcaacag gcagtcccgc gtcggaccag gtttgccagc cgccatccag
    27961 cagacgcaca tctttcacgc ctgcgtagag cataatttgc gccacacgcg ctccagcgta
    28021 tacgtcacgc ccgtacagaa tgaccgtggt gtcatggcga atgccgtgtt ttgccagcat
    28081 cgcttttagt tgttcatcag aaactttgtt ccacagcggt tcgctttcca cttcgttggt
    28141 atcgatgtag tcagcgccag gaatatggct gataaggtaa agcttaggag cgccctaggc
    28201 cgcttcaatg actttccagt caccgacagg tttcgccgta acctctttac cttgttgcag
    28261 gtcatgcagc cattgcggat aaaccagctg ctcaaagtgc ggcagttttt gtagacggga
    28321 aggttcgctt agcgcgtcac tcagaatgga gatatgcgta aaacctgctt tttgcagtcg
    28381 cgttttgacg gcgtcgacat ctttgccatt accgtacagc gccaccgggg catcggcttt
    28441 caggttatgt tgcttgatcc acgcgttgag ctgttcggtg ctcattttgt cgagccagct
    28501 agcagagagg tttaaggcgg caggttcatg accagaaggg ccatttaagg tttgtggcca
    28561 gccgttataa aacgcgctgg ggcgagtatc tatcgctttg ccattttgtt gctgaagttg
    28621 gtcaagtgta agaggcttcg ccagttcagc ggcccacgaa gaagcgcaag ccagccctaa
    28681 agccattgcc agcgcggtca tttgagaaac acgtttcatc gaaaatcccg acattgtagt
    28741 tttgagtgcg ccattctgaa ctgacgcatt gagaaaaact ttgcgttatc gcagtttgtt
    28801 gtaattttct gaccactgcg ccatatccag aacagaacta tcagcaggaa catcctggag
    28861 atcgtgcgtt acctgaacga cggggatcgc cagggcgcga acttcgctga acacccactg
    28921 gcgaaaatta tcgcgcagag ccacatcaag acggctgaat ggctcatcca ggagtaacgc
    28981 ttttggctgg gcgagaaggg cgcgtagtag agcaacgcgc gctcgctgac cgccagacaa
    29041 agtggcagga tcctgatgga aagctccgtc aaggcctgaa cgctcaagtg catcattcac
    29101 ggcattacgt cgggcattcc ccttaagtgt cgccggtagc gccagcagta aattttgccc
    29161 gacgctgaac tggtcaaata acagtgcatc ctgaaaaaga atgccaatct gacgctgtgc
    29221 ggtgggtagc atgtcaatcc gttgctcatt gagccatagc tcacctgtac aagaaaactg
    29281 tccggccagt gcaccaatca tccatgaaaa cagagtggat tttccacagc cagacggccc
    29341 cattaacgtg acgatgtcac ctttatcaac cgtaaagtta acgtttgtca gcaagcggct
    29401 ttctggtaaa cgtagcgaaa catttttcac gcagagcatt agcggattcc ttgtctgaca
    29461 taacctaccc attttgcgac taacgcggtc agggcaaaaa taataagcgg taacaacagt
    29521 tgccataacg cctgggcggc gagaataccg ttgctgccgc cgctgcttaa tgccaccgcc
    29581 tcagtggtga gcgtcggaaa acgccccgcg cccagccaca gcgtcggcat atactgcgca
    29641 atactgactg aaaatcccac cgcgaaggca gtcagcacag ggcgcaacat gagtgggcat
    29701 ttcacgtaaa agaagatttt ggcccgcgac cagcccagtg tttgcgcaat caaaattaac
    29761 cgtgaatcaa tgcgctgcca ggcgggttgc aggataaaca gcatccacgg cattacccac
    29821 agcagatgcc cccagaccac cgccgtccag cttccatcca ggttcagcca tagcgccagc
    29881 gtgtactggc ccgccaccag tggcagagcg ggcaatagaa tgggcaacca tagccacaac
    29941 tggcgacgct gtgcccccca ttccagccac agcagtaaca ggagcaaagc gatgaatgtt
    30001 gctaccagcc ccattgtcag actgttgatg agtgcttcac tattgatcgt cgactgatcc
    30061 gcgaggatcg ccagcagaac cacacacagc acaccggtta agggtaaaaa actcgccagc
    30121 gtagtgcctg gtaataaagg tgtggcaggc ttgcgaatgc catctacgcg gggaatagtg
    30181 cgccgccagc tgcgccaaaa cgtaggcggc gagtaacagc atcagcagca gactggcgag
    30241 tgcgcctttc gtttgttgat ccgcgtcgcc ctgagttaac cactgccagc taattactgc
    30301 cagcgtcggt ggattacctg gcccaagaat aatcgccaca tccacgaccg aaagcgacca
    30361 ggcaacaatc gccagcatcg ccattgccag cgaaggcgcg acggagggca acagcagcca
    30421 gttcaggcat tgccagcggc tgtagcccag cgaatccagc acaatgacct gctgcaacag
    30481 ccgtttttcg ctcaatactg ccactaagat ccacagcaga aatgcgcttt ctttcactgc
    30541 aagggtgagg cccagaccga tccaaatcgg tccattggcg gagtgaaata cgggaaatag
    30601 tcatatagca gccctccgtc agcaaagagc agcagggcgc tggtggcaaa agccacatgg
    30661 ggaatggcga gcagccacgg cagacgggcg cacatacgct gccatttcgg cccaggccat
    30721 agcgccacaa tcaccagcag ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac
    30781 accagcgttg ccagtaatgc ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt
    30841 gtcaacgaca acgcaggcga tatcagcgtg agagctgccg ggatcagcgg tgcataaatc
    30901 accgccacca tcgcccacag caaaaagatt aatgcgtacc gtaacggcgt aaccattctt
    30961 gttccagtgc attcacccat cctgcgtgcg gttcagccag taccggcggc agatcctgcg
    31021 gcattcttga ttgcaatatt tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag
    31081 aaggatcgcc ccagacaacg ggatctgctt tacgcagttg cgcatcgggt gagagcagga
    31141 aattggcaac taccttcgcc gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat
    31201 gcacgttgcc aatcatcccc tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc
    31261 cgctggcgat tttttgctgc gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc
    31321 cggctttcag cagagcatcc atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc
    31381 acaaatacgg atgcagtgca tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag
    31441 tcgcatcgtc cggcgcttct tttaatgcgg cgggatctgg cgtcagcata atcagcaact
    31501 gttcaagaaa cgccgtgccg gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat
    31561 tggctttagc aaactccagt aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc
    31621 ggcgggcgat aaacgtcagt tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca
    31681 ctgaaaaatc tccccgcacc ggcagctgtg tgtcgacata gcgccagttg ggcagagtct
    31741 ccgcccagcc cgtttgtaat aaattggcct ctttta
//
LOCUS       DAJTRB010000043        31131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTRB010000043 DAJTRB010000000
VERSION     DAJTRB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.043"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            394..2052
                     /locus_tag="OO330_002087"
     CDS             394..2052
                     /locus_tag="OO330_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5882950.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2049..3005)
                     /locus_tag="OO330_002088"
     CDS             complement(2049..3005)
                     /locus_tag="OO330_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5882951.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3156..4271
                     /gene="macA"
                     /locus_tag="OO330_002089"
     CDS             3156..4271
                     /gene="macA"
                     /locus_tag="OO330_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5882952.1"
                     /translation="MKKRKTVKKRYVIALVIVIAELITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4268..6214
                     /gene="macB"
                     /locus_tag="OO330_002090"
     CDS             4268..6214
                     /gene="macB"
                     /locus_tag="OO330_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5882953.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6287..6511)
                     /gene="cspD"
                     /locus_tag="OO330_002091"
     CDS             complement(6287..6511)
                     /gene="cspD"
                     /locus_tag="OO330_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5882954.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6834..7154
                     /gene="clpS"
                     /locus_tag="OO330_002092"
     CDS             6834..7154
                     /gene="clpS"
                     /locus_tag="OO330_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5882955.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7185..9461
                     /gene="clpA"
                     /locus_tag="OO330_002093"
     CDS             7185..9461
                     /gene="clpA"
                     /locus_tag="OO330_002093"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5882956.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9804..9891)
                     /locus_tag="OO330_002094"
     tRNA            complement(9804..9891)
                     /locus_tag="OO330_002094"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9855..9857),aa:Ser,seq:gga)
     gene            complement(10145..10363)
                     /gene="infA"
                     /locus_tag="OO330_002095"
     CDS             complement(10145..10363)
                     /gene="infA"
                     /locus_tag="OO330_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5882957.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10648..11352)
                     /gene="aat"
                     /locus_tag="OO330_002096"
     CDS             complement(10648..11352)
                     /gene="aat"
                     /locus_tag="OO330_002096"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5882958.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11394..13115)
                     /gene="cydC"
                     /locus_tag="OO330_002097"
     CDS             complement(11394..13115)
                     /gene="cydC"
                     /locus_tag="OO330_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5882959.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13116..14882)
                     /gene="cydD"
                     /locus_tag="OO330_002098"
     CDS             complement(13116..14882)
                     /gene="cydD"
                     /locus_tag="OO330_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5882960.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15005..15970)
                     /gene="trxB"
                     /locus_tag="OO330_002099"
     CDS             complement(15005..15970)
                     /gene="trxB"
                     /locus_tag="OO330_002099"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5882961.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16514..17008
                     /gene="lrp"
                     /locus_tag="OO330_002100"
     CDS             16514..17008
                     /gene="lrp"
                     /locus_tag="OO330_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5882962.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17143..21171
                     /gene="ftsK"
                     /locus_tag="OO330_002101"
     CDS             17143..21171
                     /gene="ftsK"
                     /locus_tag="OO330_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5882963.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21326..21937
                     /gene="lolA"
                     /locus_tag="OO330_002102"
     CDS             21326..21937
                     /gene="lolA"
                     /locus_tag="OO330_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5882964.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21948..23291
                     /gene="rarA"
                     /locus_tag="OO330_002103"
                     /gene_synonym="mgsA"
     CDS             21948..23291
                     /gene="rarA"
                     /locus_tag="OO330_002103"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5882965.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23382..24674
                     /gene="serS"
                     /locus_tag="OO330_002104"
     CDS             23382..24674
                     /gene="serS"
                     /locus_tag="OO330_002104"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5882966.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24914..27358
                     /gene="dmsA"
                     /locus_tag="OO330_002105"
                     /pseudo
     CDS             24914..27358
                     /gene="dmsA"
                     /locus_tag="OO330_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27369..27986
                     /gene="dmsB"
                     /locus_tag="OO330_002106"
     CDS             27369..27986
                     /gene="dmsB"
                     /locus_tag="OO330_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5882967.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27988..28779
                     /locus_tag="OO330_002107"
     CDS             27988..28779
                     /locus_tag="OO330_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5882968.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28815..29441)
                     /locus_tag="OO330_002108"
     CDS             complement(28815..29441)
                     /locus_tag="OO330_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5882969.1"
                     /translation="MTKPYVRLDKNDAAVLLVNHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29756..30904
                     /locus_tag="OO330_002109"
     CDS             29756..30904
                     /locus_tag="OO330_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5882970.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7675 a         7634 c         8358 g         7464 t
ORIGIN      
        1 aaatatggtc gttttatagg gtcgtcgtaa aaaatgacga cggaaataat gcgcggctat
       61 tttaaaaacg aaggcgagtc attcaccaga taaataaatc cagtaaattt gatttagggc
      121 aacagcgggt tgccccatat agtcatttgt ctgattgaca gtgtagtgca agcaaaagat
      181 ttaatccttt aggcgtaata aaaaataatt tatcatgcta attatttgat tttgttgttt
      241 ttgcgggctt atcagcaaga gggagtataa cgcgattatt cgctcatttt tcagacaatt
      301 gccatgctta aatgtgatgt catcacgtat tagcaaggcc tttcccgtta tactgcgagc
      361 gtaaaggata agtcacatat ttctggaggg gatatgattc ttgagcgcgt tgaaattgtg
      421 ggttttcgcg gtatcaaccg tttgtcgttg atgctcgaac aaaacaacgt cctgattggg
      481 gagaacgcgt ggggtaaatc cagcttgctg gacgccttaa ccctgctgct atcgccagaa
      541 tcagatctct accattttga gcgcgacgat ttctggttcc cgccgggaga tatcaacggg
      601 cgagaacatc atctgcatat tattttgacc ttccgcgaat cgctgccagg ccgacatcgg
      661 gttcgccgtt atcggccgct ggaagcgtgc tggacgccat gcaccgatgg ctatcaccgt
      721 attttttatc gtctggaagg ggagagtgcg gaagacggca gcgtgatgac actgcgcagt
      781 tttctcgata aagacggaca tccgattgat gttgaggata ttaacgatca ggcacgccat
      841 ctggtgcgtt taatgccggt gctgcgcttg cgtgatgccc gttttatgcg ccgtattcgt
      901 aacggcacgg tgccaaatgt ccccaatgtg gaagtcaccg cgcgccagct cgatttcctc
      961 gcccgtgagt tgtcctcaca tccgcaaaat ctctctgatg gacagattcg tcagggactt
     1021 tccgcaatgg tgcagttgct tgagcattat ttctctgagc agggggctgg acaggcgcga
     1081 tatcgtttaa tgcggcggcg ggccagcaat gagcaacgaa gctggcgcta tctggacatc
     1141 atcaaccgga tgattgaccg acctggaggg cgctcgtatc gggttatttt gctcggcctg
     1201 tttgctactt tattgcaggc aaaaggcaca ttgcgactgg ataaagacgc gcgtccgtta
     1261 ttgctaattg aagatccaga aacccgcctg catccgatta tgctttccgt tgcctggcat
     1321 ctgttgaatc ttttgccttt acagcgtatt gctaccacca actctggcga gctgctttcg
     1381 ttaactccag tggaacatgt ttgccgcctg gtgcgtgaat cttctcgtgt tgccgcctgg
     1441 cgtctggggc caagtggctt gagcaccgaa gacagccgcc gcatctcttt tcacattcgt
     1501 tttaatcgtc cgtcatcgct gtttgctcgc tgctggttgc tggtggaagg ggaaacggaa
     1561 acctgggtta tcaatgaact ggcgcgtcag tgcggacatc attttgatgc cgaagggatc
     1621 aaggtcattg agtttgccca gtccgggcta aagccactgg ttaaatttgc ccgccgaatg
     1681 gggattgaat ggcatgtact ggtcgatggc gatgaagcag ggaagaaata tgccgctacg
     1741 gtacgcagcc tgttgaataa cgatcgggaa gccgaacgag aacatttaac ggcgttaccg
     1801 gcgctggata tggaacattt tatgtatcgc cagggatttt ccgatgtgtt ccaccgcgtg
     1861 gcgcaaatcc cggaaaatgt accgatgaat ctacgcaaaa ttatctcgaa agcgatccat
     1921 cgctcttcca aacccgatct tgccattgaa gtggcaatgg aggcaggacg tcgtggtgtg
     1981 gactccgtac caacgctgct gaaaaaaatg ttctcacgcg tgctgtggct ggcgcgcggt
     2041 cgcgcggatt aaccgcgaaa catcgtggcc atttgtggct gaatagcgtc gagcatctca
     2101 tagcgccgac ggtattcagc ccgtttttta ctggcgattt cggcaatctc ttttcgtgct
     2161 atctgtgctg gaaggcggta atggcgttca gcatcacata cgccgccaac cgactcccag
     2221 aacgcgttgt aatcagcatg gatcttgcct tctttatcgc gataacgcag gctgcggtaa
     2281 atatgcgttt cattgctgac ggcaataatc tgctctacct gcaaacgttg ggcaaacaga
     2341 caggccgctt ccatcacgag gcgtttggga aatagcccgt ggcaggcttt cgtcgcattc
     2401 tggatttcct gatgtggaat ttcccatttt gcgccttgca gtccgccaat aaacatcgtt
     2461 cttttcccct gatattcaca cagggtaaac gtgatctctg ccagaggaat accttcgctg
     2521 ttgcggaaca ggattgtgct gtcaccttct ttatccattg agatcatcat ggtcagctca
     2581 agcgtgaact gctcaccgtt tttgccttcc agtttagcca gttgcagccc gggggtattc
     2641 aaatataagc tgaattcttc cgccgacata cacccgcgga gtaacgcata atggtaacgt
     2701 aacgcctcca gcaattgctt acggctaaga ttcgcggcaa ggtaagggcg atgcagacgc
     2761 acaggcagtc gcggctggcg cgttaacaac acattgagat taggccagtg ggaaagttcg
     2821 ttcatccact caacgcttaa acgcggcata atcaacgagc gcagcaaaaa tttctggcga
     2881 aaactacagc gatgccagaa tttacccggc cgacactgtc cacgtgccag actaagaaaa
     2941 agtgacaggc tgctgagaga ttcagatggc gtaaaggtcc gttcagttag ctgcgacata
     3001 ttcatgaaat caatggttat acatgacgtc gatttcacca ttgcgtatct taaccaaaca
     3061 tcaatagtgt gattactaac gtaaatttta gggttttgtt gatatttcgt tgaagttaat
     3121 gacccggatt ggcatatgga gtattcagaa tatttatgaa aaagcggaaa accgtgaaga
     3181 agcgttacgt tattgcgctg gtgatagtca tcgccgaact gattacgtta tggagaattc
     3241 ttaacgcacc cgtgccgact tatcagacac tgattgtgcg ccccggtgat ttacagcaaa
     3301 gcgtgctggc gaccggaaag ctggacgcgc tgcgtaaggt tgacgtgggc gcgcaggtca
     3361 gcggtcagtt gaaaacgctg tcggtggcga ttggcgataa agtaaaaaaa gaccagcttt
     3421 taggggtcat tgatcctgaa caggctgaaa accagatcaa ggaggtcgaa gcaacgctga
     3481 tggagctacg cgcgcagcgg cagcaggcgg aagcggagct gaaactggcg cgggtgacgt
     3541 attcccgtca gcaacgtctg gcacaaacgc aagcggtttc actgcaggat ctcgataccg
     3601 ccgcgacgga gatggctgtg aaacaggcgc aaattggcac cattgacgcg caaatcaagc
     3661 gcaatcaggc ctctctcgat acggctaaaa ccaatctcga ttacacccgt atcgttgccc
     3721 cgatggctgg ggaagtgacg caaatcacca ctctgcaagg ccagacggtg attgccgcac
     3781 agcaagcacc gaacattctg acgctggcag atatgagcac tatgctggta aaagcgcagg
     3841 tttctgaagc ggatgtaatc cacctgaagc cggggcaaaa agcctggttt acggtgcttg
     3901 gcgatccact gacgcgctac gaggggcaaa tcaaggatgt gctaccgacg ccggaaaagg
     3961 taaacgacgc tattttctat tacgcccgtt ttgaagtacc caaccccaat ggtttgctgc
     4021 ggctggatat gaccgcgcaa gtgcatattc agctcaccga tgtgaaaaat gtgttgacga
     4081 tccctctgtc ggcgttaggc gatccggttg gcgataatcg ttataaagtc aaattgttgc
     4141 gtaatggtga aacacgcgag cgtgaagtga cgattggcgc acgtaacgat accgatgttg
     4201 agattgtcaa agggctggaa gcgggcgatg aagtggtgat tggtgaggcc aaaccaggag
     4261 ctgcacaatg acgcctttgc tcgaattaaa ggatattcgt cgcagctatc ctgccggtga
     4321 tgagcaggtt gaggtgctga agggcatcag cctcaatatt tatgcgggtg agatggtcgc
     4381 gattgttggc gcttcgggtt ccggtaaatc gaccctgatg aatattctcg gctgcctgga
     4441 taaggccacc agcggcacct atcgcgtagc cggtcaggat attgccacgc tggacgccga
     4501 tgcgctggcg caactgcgcc gcgagcattt cggctttatt ttccagcgtt accatttgct
     4561 tccgcattta accgccgagc agaacgttga agtacccgcc gtctatgccg gtcttgagcg
     4621 gaaacagcga ctgcttcgtg cccaggagtt gctgcaacgg ctggggctgg aagaccgtac
     4681 agagtattat ccggcacagc tttcgggtgg tcagcaacag cgcgtcagca tcgcgcgggc
     4741 attgatgaac ggcggtcagg taattcttgc cgatgaaccg accggcgcac tggacagcca
     4801 ttctggcgaa gaggtgatgg cgatcctgca tcagctgcgc gatcgcgggc atacggtgat
     4861 tatcgtcacc cacgatccgc aggtcgctgc tcaggccgag cgggtgatcg aaattcgcga
     4921 cggcgaaatt gtgcgcaatc ctcccgccat tgaaaaagtg aatgttgctg gcgggacgga
     4981 gcctgttgtc aatacagtgt ctggctggcg gcagtttgtc agcggtttta acgaggcgct
     5041 gacgatggca tggcgggcgc tggcagcgaa taaaatgcgt actttactga ccatgctggg
     5101 gattattatc ggtattgcgt cggtggtttc cattgtcgtg gtgggtgacg ccgccaaaca
     5161 aatggtgctg gcggatattc gttctattgg tacgaatact attgatgtct atcccgggaa
     5221 agattttggc gatgacgatc cgcaatatca gcaggcgctg aagtacgacg acttaatcgc
     5281 catccaaaaa caaccgtggg tcgcctcagc cacacctgcc gtctcgcaaa acctgcgcct
     5341 gcgttataac aatgttgatg ttgctgccag tgccaatggc gtgagcggcg attattttaa
     5401 tgtctatggc atgaccttca gtgaaggaaa cacctttaat caggagcagc tgaacggtcg
     5461 tgcgcaggtc gtggttctcg acagtaatac tcgccgccag cttttccccc ataaagcaga
     5521 tgtggttggc gaggtgattc tggtcggcaa tatgcccgcc agagtcattg gtgtggcgga
     5581 agaaaaacag tcgatgtttg gtagcagtaa agtgctgcgt gtctggctac cttacagcac
     5641 gatgtccggg cgagttatgg gccagtcgtg gcttaactcc attactgtca gggtgaaaga
     5701 aggatttgac agcgccgagg cggaacagca actcacgcgt ttactttcac tgcgccacgg
     5761 taagaaggat ttctttacct ggaacatgga cggcgtcttg aaaactgttg aaaagaccac
     5821 acgtacttta caactgtttc tgacgctggt ggcggtgatt tcgctggtgg tgggcggtat
     5881 tggtgtaatg aatattatgc tggtgtcagt gaccgagcgg acgcgggaaa ttggcattcg
     5941 catggctgta ggtgcgcgag caagcgatgt tttgcaacag ttcctgatcg aagccgtact
     6001 ggtttgcctg gtcggtggcg cgttgggaat aacactgtca ctgttaattg ctttcacctt
     6061 gcagcttttc ttacccggct gggagattgg tttttcaccg ttggcgctgc tgctggcgtt
     6121 tctctgctcg acggccaccg ggattttatt tggctggtta cccgcacgaa atgcggcacg
     6181 actggatcca gtagatgctc tggcacggga gtaatttttg agataaaaat gccagccgat
     6241 cgggctggca ttttgccttt aggatgtaca caatgagaca gaagagctat gcgactgccg
     6301 cttctacttc gacgggcaca ataacactgg cgtgattgcc ttttggcccc tggtggacat
     6361 caaactgaac ggattgtcca gcttttagcg ttctgtaacc atccatctga atggtggaat
     6421 aatgagcgaa aatatcttcg ccgccgcctt cagggcagat gaaaccaaac cctttggcat
     6481 tgttgaacca cttaacagta cccttttcca tgcttcgaca tccttcgcaa atcttataca
     6541 agtaagatgg aataaaccgg ggtcagagag ggggctgttc aaaacctcgc caactctaga
     6601 aatacaattt agagaattag ggcgagccgt caagcatttg acaggggcca gggggcaggt
     6661 atgaatcaaa aatttgaagc agttaacgct attgacagga atgtgacaga tgtcgctgat
     6721 gccaacgata gatgatagtt atctatcatg tggagtagat tggtcaggca aataagctct
     6781 tgtcagcggc agggcgttct gccgataacc gtaaccgaag atgataactg acaatgggta
     6841 aaacgaacga ctggctggac tttgatcaac tggcggaaga aaaagttcgc gacgcgctaa
     6901 aaccgccatc tatgtataaa gtgatattag tcaatgatga ttacactccg atggagtttg
     6961 ttattgacgt gttacaaaaa ttcttttctt atgatgtaga acgtgcaacg caattgatgc
     7021 tcgctgttca ctaccagggg aaggccattt gcggagtctt taccgccgag gttgcagaaa
     7081 ccaaagtggc gatggtgaac aagtacgcga gggagaatga gcatccattg ctgtgtacgc
     7141 tagaaaaagc ctgaatgcag gcataaaaat tgggggaggt gcctatgctc aatcaagaac
     7201 tggaactcag tttaaatatg gctttcgcca gagcgcgcga gcaccgtcat gagtttatga
     7261 ccgtcgagca cttgttactg gcgctgctca gtaacccatc tgcccgggag gcgctggaag
     7321 cgtgttctgt ggatttggtt gcgctccgtc aggaactgga agcctttatt gaacaaacca
     7381 cacccgttct gcctgccagt gaagaggagc gcgacacaca gccgacgctg agttttcagc
     7441 gtgtactgca acgtgcggtc ttccatgtcc agtcctccgg tcgcaatgag gttaccggtg
     7501 caaacgttct ggtcgctatc ttcagcgaac aggagtcgca ggcggcatat ctgttgcgta
     7561 aacatgaagt cagccgtctc gatgtggtga actttatctc tcatggcacg cgtaaagacg
     7621 agccgacaca gtcttctgat cctggcagcc agccaaacag cgaagaacaa gctggtgggg
     7681 aggaacgtat ggagaatttc acgacgaacc tgaaccagct tgcgcgtgtg ggcggaatcg
     7741 acccactaat tggtcgtgag aaagagctgg agcgtgctat tcaggttctc tgccgtcgcc
     7801 gtaaaaacaa cccgctgctg gtgggggaat ctggtgtcgg taaaaccgcg attgcagaag
     7861 gtcttgcctg gcgaattgtt cagggtgatg tgccggaagt gatggctgac tgtacgattt
     7921 actctctcga tatcggttct ctgttagcgg gcacaaaata tcgcggcgac tttgaaaaac
     7981 gttttaaagc gttgctcaag cagctggagc aggacactaa cagcatcctg tttattgatg
     8041 agatccacac cattatcggt gcgggtgcag cgtctggtgg tcaggtcgat gcggctaacc
     8101 taatcaaacc gttgctctcc agcggtaaaa ttcgcgtaat tggttcgaca acctatcagg
     8161 agttcagcaa cattttcgag aaagaccgtg ctctggcgcg tcgcttccag aaaattgata
     8221 ttactgaacc gtcgatcgaa gaaactgttc aaatcatcaa tggcctgaaa ccgaagtatg
     8281 aagcgcacca cgacgtgcgt tataccgcaa aagcggtgcg tgcggcggta gagctggcgg
     8341 tgaaatacat taacgatcgt catctgccgg ataaagccat tgatgttatc gacgaagcgg
     8401 gcgctcgcgc acgtctgatg ccggtaagca aacgcaagaa aaccgttaat gtggcggata
     8461 ttgagtccgt ggtggcccgt attgcacgca ttccagagaa gagtgtttct cagagtgacc
     8521 gcgataccct gaaaaaactc agcgatcgcc tgaaaatgct ggtcttcggc caggataaag
     8581 ccattgaggc gctgactgaa gccattaaga tggcgcgtgc aggtttaggt cacgaacata
     8641 aaccggtcgg ttcgttcctg tttgccggtc ctaccggggt cgggaaaaca gaggtgacgg
     8701 tacagctttc gaaagcgttg ggcattgagc ttctgcgctt tgatatgtcc gagtatatgg
     8761 aacgccatac cgtcagccgt ctgattggtg cgcctccggg atacgttggt tttgatcagg
     8821 gcggtttgct gaccgatgcg gtaatcaagc atccacatgc ggtgctgctg ctggacgaaa
     8881 tcgagaaagc gcatccggac gtgttcaata ttctgttgca ggtgatggac aacggtacgc
     8941 tgaccgataa caacggacgc aaagcggact tccgtaacgt ggtgctggtg atgaccacta
     9001 acgccggggt acgggaaact gagcgcaaat ccattggtct tatccaccag gataacagca
     9061 ccgatgcgat ggaagagatc aagaagatct ttacaccgga attccgtaac cgtctcgaca
     9121 acattatctg gttcgatcat ctgtcaactg acgtgatcca tcaggtggtg gataaattca
     9181 tcgtcgagtt gcaggttcag ctggatcaga aaggtgtttc tctggaagtg agccaggaag
     9241 cgcgtaactg gctggccgag aaaggttacg accgggcaat gggcgcacgt ccgatggcgc
     9301 gtgtcatcca ggacaacctg aaaaaaccgc tcgccaacga actgctgttt ggttcgctgg
     9361 tggacggcgg tcaggtgacg gttgcgctgg ataaagagaa aaatgagctg acttacggat
     9421 tccagagtgc acaaaagcac aaggcggaag cagcgcatta atctgattgt caggtaggtt
     9481 ggtgaagtcc gtaatctcgt caggggttac ggacttttta tttatggggt ggaacggtga
     9541 aaaccctatt tttggaggtg aaggtaagtt gttgataatt agtgctgctg gaaggtaagg
     9601 ataaaaaagg gtgctgcagg agaatgggat ggttttgctt tattaacaac gggctaaacg
     9661 tgtagtattt gagttcactg ccgtacaggc agcttagaaa ttcacaggta acatactcca
     9721 cccgcccacc atgttcactg ccgtacagac agataaaatg cgaaaaaaaa gctcgcactt
     9781 tcgtacgagc tcttctttaa atatggcggt gaggggggga ttcgaacccc cgatacgttg
     9841 ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac caaattgttt
     9901 tgctaccaaa cctcatgggt ggcaacgggg cgctactata gggagttgga gtaaaacggt
     9961 caagaagaat tttaatgata attattgttt gctcatactg taaacaactt gtgcagtata
    10021 tctacatcga gacaagttat ggacttatac ttccaaagta cttcatacat atcacaaaat
    10081 aaaaaggccg gttaaaccga ccttttactc gttctttctc ttcgcccatc aggcggtaaa
    10141 acaatcagcg actacggaag acaatgcggc ctttgctcag gtcgtacggg gtcagttcaa
    10201 cagtcacttt gtcgcccgtc aggatgcgga tgtagttttt gcgcatttta ccggagatgt
    10261 gtgcagtaac cacgtgaccg ttttctaact ctacgcggaa catggtatta ggcaacgttt
    10321 caagaacggt accttgcatt tcaatattgt cttctttggc catctaatcc tctggggtat
    10381 cactaccgta atttgaaccg gcaagataat gccgaagttc tgtaaataag taaagatttg
    10441 cgcgctaaat cgcaacaaac aggttcggca cattactccg aaaacacacg gctaagccgc
    10501 accaaaagcg caacgtataa gggagcggtg agataaacga tgggcgttac ctgacgcgaa
    10561 aaattcctta tcggcagcgg ggtaatgagc gtaaccaact ctgcgaccgc aattataaca
    10621 ctctggggag aaatgtgccg aaaacattca ttcttgtggt gaaaacaagc accgtggtac
    10681 ccagaaatta ttcggcaatc gtccgaggcg catttgattg agataattaa ggtaatcccg
    10741 gcggggaatt tcgcaggcac caagcgatgc tgtgtgatcg ttaaggacct ggcagtcgat
    10801 aagcttaccg ccatgaccga taaattcctc acagaatacc agaagcgccg ttttagacgc
    10861 attttccatc cggctgaaca tggactcgcc acaaaatagc gttccctggg ccacgccgta
    10921 cataccgccg acaagctcat cttcacgcca gacttcaatg gagtgggcat gaccgagttc
    10981 gtgaaggcga tggtaggctt cgaccacgcc acgcgtgatc caggttcctt cttcgcgatc
    11041 gctggcacag ccttcaatga cctgaccaaa agcgtaattc atcgtgacac gatagggcga
    11101 gcgtttatga aatcgcttca tactacggct gatatgcagt gattctggcc atagcaccgc
    11161 gcggggatcg ggcgaccacc agaggatggg gtcgcctgga gaaaaccacg gaaaaatacc
    11221 acgctggtaa gccattaaca ggcgcgcagg gctaagatcg cccccaagtg ccagcaggcc
    11281 gttaggctca cgtaatgcgc cttccgggga agggaaggct attgaatggc gagaaagctg
    11341 aaccaagcgc atgaccgcaa aactccacgc aagtcggatc gttcaataat agcttacaaa
    11401 ccctgcttga actggtaata acgcccctgt ctggcaagca gttctgcgtg agtaccttgc
    11461 tcaataattt gcccgttgtc catcactatt atttgttgga aacgagagag tccgcgaagt
    11521 cgatgggtga ccattaacac cgttttctca cgcatcattt ttgcaagcaa ttcaaggatc
    11581 tggctttcgg ttgtggcatc taagccttcg gtaggttcat ccagcaacac cagtggcgca
    11641 tcatgtaaca gcgtacgggc gatagccaga cggcgcagtt caccaccgga gagctggcgt
    11701 ccgccttcac ctaaccaact gttgagacct gcatcctcga gcagcttctc cagaccaacg
    11761 cgacgcaaga tctccgacag agcctcatca ctactgccag gcgaggcgag taacagatta
    11821 tcacgcagcg tggcgctaaa cagatgcact cgctgaggaa caacgctgat ggtctgtcgt
    11881 agagccgctt cattcaggct ggctatgggg ctatcgttaa gcaaaatctc gccctgttgc
    11941 gggtcccatg cgcgggtcag cagttgtaac agtgttgatt tgccgcatcc ggttcgcccg
    12001 agaatcgcta tatgttcccc ggcgtttacc tgaagagaaa tccctttaag tgcctgttgg
    12061 gattgctccg gataagtgaa ctgaacatcc cgtaatgtca gcgaaacgcg atcggcaaca
    12121 cgagtttggg tatcagggaa ggtgacctcc ggtttttgat ccgttaagtc agagatacgt
    12181 acggcagagg caatgacttg ccccagatgc tgaaatgcac ccgttactgg tgccagtgct
    12241 tcaaacgcgg ctaacgcaca aaagacaaac agggcaatta atgcgccggg ttgagcattg
    12301 ccgccaacgc cgccagacgc catccacagc atcaggatca ccgctaacgc gccaatgagc
    12361 agcattatcg cttgcgacaa tgcggtcagt tcagattgac ggcgttgcgc ttccagccat
    12421 tgaatttctg tattctctag ttgcgtgcga taacgatcgc tggcaccaaa aatggtcagc
    12481 tcagcttgcc cttgcagcca ggccgtcagt tgttggcgat actgtccgcg aagatgagtc
    12541 agattttgcc cggtgctttt tcccgcacga taaaacagcg gtggcatcag gaaaagcgtc
    12601 agtaacataa cgctgcccag cgtaaaggcg agggtgaaat caaggaaact taacccgatt
    12661 gtcaccacca taatcaccac aaaagcgccc accagcggcg agataacgcg caggtaaaga
    12721 tgatcgagcg tatcaacatc cgccaccacg cggttgagca attcgccctg acgatagcgc
    12781 gccagtccgg caggggagag gggcaacaat ttgctgaagg tgtaaatgcg cagatgctgc
    12841 aacacgcgga aagtcgcgtc gtgacttacc agacgttcaa aataacgccc ggcagtacgg
    12901 gtgattgcgg caccacgcac gcccgcagcg ggtagcatat agttgaagct gtacagtccg
    12961 gcaacccccg caaccgctga ggccgagagg aaccagccgg aaagtgtcaa cagaccgata
    13021 ctggcgagca gcgtcacaat tgccagcaca ataccaagac ttaacatcca tttatgacgt
    13081 ttatacagtg ccagataggg tagcaaagcg cgcatttaaa tctcctcctg acgatgggcc
    13141 agtaatgtgg cgaatgggcc accagccaca cttaattccg cgtaacgtcc ttgctcaata
    13201 atccggccat cctgcataac ccaaatgaca tcccagtcag caagatcttc taactggtgg
    13261 gtgaccatta acgttgtctg gcgcagagag gcggcattca gcgcctccat tacgcgctgt
    13321 tcactgtgag catcaaggct ggcagcgggt tcatccaaca gtaatagcga acagggattt
    13381 agtaacgcac gggccaccgc cacgcgctgc gcctgcccca cggaaaggcg ggcagcctgg
    13441 tcgccaacag gtgtatcaac gccttgtggc aggagcggta gaaactcgct gacccaggcg
    13501 ttatccagcg ctgcttgtaa ttcttgttcg ctggcatcag gtcgcgccag tagtacgtta
    13561 tcccgcaatt ttgctgccgg taattgtggg ttttgcccaa cccaggagag atgtttacgc
    13621 catgattctg gtgataaatc acgtaattct atcccgttga ttcgtaatga tccctgatat
    13681 gagagaaaac cagaaagcgc gttcagcagt gagcttttac ctgaaccgct gcggccaacc
    13741 aacactgcac gttggcctgc tggcaaagta aagttcagcg gtccggccag cgttttacct
    13801 tccggcgacg tgataaacag atcctcggcc tcaatggtca gcggatcggt caatgctaat
    13861 tccgcctcac cgcgctgcgg atgggcgagc ggggtttcca taaacgtttt cagactgtca
    13921 gctgcaccaa cagcctgggc tttagcatga taaaacgtac cgagatcgcg taatggctgg
    13981 aaaaactctg gcgcaaggat cagggccaga aaacctgcag ccagcgtcac cccagtatcg
    14041 tagtgaccaa aatccagctc gccgagatag gaaaaaccaa agtagaccgc caccagagca
    14101 attgacagcg aggtaaaaaa ttcgagaatg ccggaggata aaaacgccag tcgtagcact
    14161 tccattgtcc gttggcggaa atcttccgaa gcagaacgaa tactttcaat ttcagcttca
    14221 ccacgaccaa aaatacgcaa tgtttccatg ccgcgcaggc gatcgaggaa atgcccactt
    14281 aagcgagcaa gagcgagaaa gttacgccgg ttagcatcgg cagcccccat tccaaccagc
    14341 gccataaaca acggaattag cggtgctgtg cccagcagaa tgagcgccgc agcccagtta
    14401 gaggggaaga tcgccaccac aatcagcaac ggcaccgaca ctgccagcgc catttgcggc
    14461 agatagcgtg catagtaatc atgcatatcg tcaatttgct cgagtaccag cgtcgcccag
    14521 ctccccgcag gtttaccctg aatccacgct ggccctgctt gttgcagacg gtcgagaacc
    14581 tgacggcgga tggcaaagcg gatatgctgc ccggcgtgat aacccacccg ttcgcgtaac
    14641 cagaccaccc atgcgcgcaa tacaaaggtc agaaccagta acgtaaaggg aagcagcagg
    14701 gcttcacggg gaatattctc cataatcata tgttgcagaa ttcgcgccat gaaccaggcc
    14761 tgggcaatga tcaatatgcc gcttacaaag cccagcagac gagaaatatt cagccaacgt
    14821 tgggagatga cgctttgctg ttttaaccag cgggttaact ctttttgacg agatttattc
    14881 attgcacgct tagcaggtga gttatcagaa ttatttgcag agcaatgtta caacggggaa
    14941 aaaataaagg cgacccatag tcgcaaggtg tcgccttctt tacttttgtt actgatttgt
    15001 aaaattattt tgcgtcagct aaaccatcga ggtagcgttc cgcatcaagt gctgccatgc
    15061 agccagtacc ggccgaagta atggcctggc gataaatgtg atccatcacg tcgcctgcgg
    15121 caaagacgcc aggaatgctg gtctgggtgg cattaccatg aatacccgac tgtactttga
    15181 tgtagccgtt ttccagttcc agctgccctt cgaaaatcgc agtattcggg ctgtgaccga
    15241 tagcaacaaa cagaccggca acgtcgagtg actcgatgtt atcgctgttt tgcgtatcgc
    15301 gcagacgaac gccagtgaca cccatttgat cgccggtcac ttcttccagc gtacggttgg
    15361 tgtgcagaat gatgttgccg ttctccactt tatccatcag acgcttaata aggatttttt
    15421 ccgcgcggaa accgtcacgg cggtgaatca gatgcacttc cgaagcgatg ttagacagat
    15481 acagcgcctc ttcaaccgcg gtattgccgc cgccgatgac cgcaactttc tggttgcgat
    15541 agaagaaacc gtcgcaggtt gcacaagcag aaaccccacg gcctttaaag gcttcttcag
    15601 agggcaggcc gagatagcgt gcagaagctc cggtggcaat aatcagcgcg tcgcaagtgt
    15661 attcgccgtt atcgccattc agacggaacg gacggttttg cagatccacc ttgttgatat
    15721 gatcaaaaat gatctcagtt tcaaacttgg tggcatgttc gtgcatgcgc gccattaata
    15781 acggaccggt cagatcgttt ggatcaccag gccagttttc cacttccgtg gtggtggtca
    15841 gttggccgcc tttttccatg ccggtaatca gcacaggttg caggttggcg cgcgccgcgt
    15901 agacagcagc ggtgtatccc gccgggcctg aacccaggat aagcagttta ctgtgtttgg
    15961 tcgtgcccat gagatcccca tagttgttgg cagacaatgg gcaggattgt agggaattta
    16021 cagacgtaaa aaaagagtat gacgattttg ttaacaattt gtgcaatcgg cagcatcgat
    16081 aagcaggtca aattctcccg tcattatcac ctctgctact taaatttccc gctttataag
    16141 ccgattaaat gatgaataaa cgcccctgtt aatgaatatc tggcatgttg tactaaaaat
    16201 cgatgttttg ctttgacaat cccctggtgt tttgcgaaaa cattcgagga agaaaaaaac
    16261 agtattctta tatgcgcata accatgtatg taaataccat gtttaccgtg ctagtgaaat
    16321 ctacgtatgg cgtggacaga cgccattcgt gatgtcgata gctgccacaa ggcaacggtc
    16381 ttctcaccgt agacccaggc attgcgcgcc gtgaatcttc atgatttcgg tctatcgtga
    16441 cgggtagcga ctctgaacag tgatgtttca gggtcagaca ggagtaggga aggaatacag
    16501 agagacaata ataatggtag atagcaagaa gcgccctggc aaagatctcg accgtatcga
    16561 tcgtaacatt cttaatgagt tgcaaaagga tgggcgtatt tctaacgtcg agctttctaa
    16621 acgtgtggga ctttccccaa cgccgtgcct tgagcgtgtg cgtcggctgg aaagacaagg
    16681 gtttattcag ggctatacgg cgctgcttaa cccccattat ctggatgcat cacttctggt
    16741 attcgttgag attactctga atcgtggcgc accggatgtg tttgaacaat tcaataccgc
    16801 tgtacaaaaa cttgaagaaa ttcaggagtg tcatttagta tctggtgatt tcgactacct
    16861 gttgaaaaca cgcgtgccgg atatgtcagc ttaccgtaag ttgctggggg aaaccctgct
    16921 gcgtctgcct ggcgtcaatg acacacggac atacgttgtt atggaagaag tcaagcagag
    16981 taatcgtctg gttattaaga cgcgctaaca cggaacaggt gcaaaatcgg cgtattttga
    17041 ttacactcct gttaatccat acagcaacag tactggggta acctggtact gttgtccgtt
    17101 ttagcatcgg gcaggaaaag cctgtaacct ggagagcctt tcttgagcca ggaatacact
    17161 gaagacaaag aagtcacatt gacaaagtta agtagcggcc gccgccttct ggaggcattg
    17221 ctgatcctta ttgtcctgtt tgccgtctgg ttgatggctg ccttactaag ctttaaccct
    17281 tcggacccca gctggtcgca aacggcctgg catgaaccta tccataattt aggtgggatg
    17341 cccggtgcgt ggttggcaga tacgctgttc tttatttttg gcgtgatggc ttacaccatt
    17401 cccgtcatta ttgtcggcgg ttgttggttt gcctggcgtc atcagtccag cgacgaatac
    17461 attgattatt ttgccgtttc gctacgcatc attggcgttt tggcgctcat ccttacctcc
    17521 tgtggtctgg cggcaatcaa cgctgacgat atctggtatt ttgcctccgg tggcgtcatt
    17581 ggcagcttac taagcactac gctacaacca ctgctacaca gtagcggggg aactattgcg
    17641 ctgctctgcg tttgggcagc gggcctgacg ttgttcaccg gttggtcatg ggtgaccatt
    17701 gctgaaaaac tcggcggctg gattttaaac attctcacct tcgccagtaa tcgtacccgt
    17761 cgcgatgata cctgggtcga tgaagatgag tatgaagacg acgaagagta tgaagatgaa
    17821 aatcacggca aacagcatga atcacgccgt gcccgtattc ttcgcggcgc gctagcgcgt
    17881 cgtaaacggt tggcggaaaa attcattaat ccgatggggc ggcaaacaga cgctgcgttg
    17941 ttctccggta agcggatgga tgatgacgaa gagattacct acactgcacg cggtgtggct
    18001 gccgacccgg acgacgtcct attttcgggc aatcgtgcaa cgcagccaga atatgacgaa
    18061 tacgatccat tattaaacgg tgcgccaatt accgaacctg tcgctgtagc agctgctgct
    18121 accacggcga cacaaagctg ggctgcgccg gttgaacctg tgactcagac gccgcctgtt
    18181 gcctctgttg atgttccacc ttcgcaacct acagtagcct ggcagcctgt accgggtcca
    18241 caaacgggag agccggttat tgctcctgca ccggaaggtt acccacagca gtcacaatat
    18301 gcgcagcctg cagtgcaata taatgagccg ctgcaacaac cagtacagcc gcagcagccg
    18361 tattatgcac ctgcagctga acaacctgcg caacagccgt attatgcccc tgcgccagaa
    18421 caaccggtgg caggtaacgc ctggcaagcc gaagagcagc aatccacttt tgctccacag
    18481 tctacatacc agactgagca aacttatcag cagccagccg ctcaggagcc gttgtaccaa
    18541 cagccgcaac ccgttgaaca gcagcctgtt gtggagcctg aacccgttgt agaagagaca
    18601 aaacccgcgc gtccgccgct ttactatttt gaagaagtgg aagagaagcg agcccgtgaa
    18661 cgtgaacaac ttgcggcctg gtatcaaccg attccagaac cggttaaaga accagaaccg
    18721 atcaaatctt cgctgaaagc accttctgtt gcagcagtac ctccagtaga agccgctgcc
    18781 gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg ggctgccgca
    18841 accgttgccg cgccagtctt cagtctggca aatagcggtg gaccgcgtcc gcaggtcaaa
    18901 gaggggattg gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac tcgtcgtgaa
    18961 ttggcgtctt acggtattaa gctgccctca cagcgtgcgg cggaagaaaa agcccgtgaa
    19021 gcccagcgca atcagtatga ttctggcgat cagtataacg atgatgaaat cgatgcgatg
    19081 cagcaggatg aactggcacg tcagttcgcc cagacacagc agcaacgcta tggcgaacag
    19141 tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga ggctgaactg
    19201 gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg gcgaacaacc ggctggggcg
    19261 aatccgttct cgctggatga ttttgaattt tcgccaatga aagcgttgct ggatgatggt
    19321 ccacacgaac cgctgtttac gccaattgtt gaacctgtac agcagccgca acaaccggtt
    19381 gcaccacagc agcagtatca gcagccacaa cagccggttg cgccacagcc gcagtatcag
    19441 cagccacaac agcaggttgc gccgcagcca caatatcagc agccgcaaca accggttgcg
    19501 ccacagcagc aatatcagca gccgcaacaa ccagttgcgc cgcagccgca gtatcaacag
    19561 ccgcaacaac cggttgcacc gcagccgcag tatcagcagc cacaacagcc agttgcgcca
    19621 cagccgcagg ataccctgct tcatccgctg ttgatgcgta atggcgacag ccgtccgttg
    19681 cacaaaccga cgacgccgct gccttctctg gatttgctga caccgccgcc gagcgaagtg
    19741 gagccggtag atacctttgc gcttgaacaa atggcgcgtc tggtggaagc gcgtctggct
    19801 gatttccgta ttaaagccga tgtcgttaat tactctccgg ggccagttat cactcgcttt
    19861 gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt ctaacttgtc acgggacctt
    19921 gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg caaaccctat
    19981 gtaggtctgg agttaccaaa taaaaaacga caaaccgttt atctgcgcga agttttggat
    20041 aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa agatatcgcc
    20101 ggtgagccgg tggttgccga tctggcgaaa atgccgcact tgttggttgc ggggactacc
    20161 ggttccggta aatctgtcgg tgtgaacgcg atgatcctga gcatgcttta taaagcacag
    20221 ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc tggagctttc ggtttatgaa
    20281 ggcattccgc acctgttaac ggaagtcgtt actgatatga aagatgccgc caacgcgctg
    20341 cgctggtgtg ttaacgagat ggagcgtcgg tataaactga tgtctgcgct gggtgtgcgt
    20401 aatctggcgg gttataacga aaaaattgct gaagccgatc gcatgatgcg tccgattcca
    20461 gacccgtact ggaagccggg tgacagtatg gatgcccagc atccggtgct gaaaaaagaa
    20521 ccatacattg tcgtgttggt tgacgaattt gccgacctga tgatgacggt aggtaaaaaa
    20581 gtggaagagc tgatagctcg tctggcgcaa aaagcccgtg ccgcgggtat ccacctcgta
    20641 ctggcaactc agcgtccatc ggttgatgtt attactggtc tgattaaagc gaatattccg
    20701 acccgtatcg cctttaccgt atccagtaag attgactcac gtaccattct tgatcaggct
    20761 ggcgcggaat cactgctggg tatgggggat atgctctact ctgggccgaa ctccacgttg
    20821 ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag ttcatgccgt ggtgcaggac
    20881 tggaaagcgc gtggtcgccc acagtatgtt gatggcatca cctccgacag cgaaagcgaa
    20941 ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc cgttgttcga tcaggcggtg
    21001 cagtttgtca ctgaaaaacg caaagcgtca atttctggcg tacagcgtca gttccgcatt
    21061 ggttataacc gtgcagcgcg tattatcgaa cagatggaag cgcaggggat tgtcagcgaa
    21121 caggggcaca acggtaatcg tgaagtgctg gccccaccgc cgtttgacta attaatgcat
    21181 tgccggataa ggcgcggtag cgtcgcatcc ggcactctat caactgaaaa ttcagtattt
    21241 tcttctttcc tcaagctgat tattagcctg gaatagagag tagagggaac tcccgatcgg
    21301 gagtgacgta atttgaggaa taatgatgaa aaaaattgct atcacctgtg cattactctc
    21361 aagcttagta gcaagcagcg tttgggctga tgccgcaagc gatctgaaaa gccgcctgga
    21421 taaagtcagc agcttccacg ccagcttcac acaaaaagtg actgacggta gcggcgcggc
    21481 ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca aacttattca actggcatat
    21541 gacacaacct gatgaaagca ttctggtttc tgacggtaaa acactgtggt tctataaccc
    21601 gttcgttgag caagctacgg caacctggct gaaagatgcc accggtaata cgccgtttat
    21661 gctgattgcc cgcaaccagt ccagcgactg gcagcagtac aatatcaaac agaatggcga
    21721 tgactttgtc ctgacgccga aagccagcaa tggcaatctg aagcagttca ccattaacgt
    21781 gggacgtgat ggcacaatcc atcagtttag cgcggtggag caggacgatc agcgcagcag
    21841 ttatcagctg aaatcccagc aaaatggggc tgtggatgca gcgaaattta ccttcacccc
    21901 gccgcaaggc gtcacggtag atgatcaacg taagtagagg cacctgagtg agcaatctgt
    21961 cgctcgattt ttcggataat acttttcaac ctctggccgc gcgtatgcgg ccagaaaatt
    22021 tagcacagta tatcggtcag caacatttgc tggctgcggg gaagccgttg ccgcgcgcta
    22081 tcgaagccgg gcatttgcat tctatgatcc tctgggggcc accgggtacc ggcaaaacaa
    22141 ccctcgctga agtgattgcc cgctatgcga acgctgatgt ggaacgtatt tctgccgtca
    22201 cctctggcgt gaaagagatt cgcgaggcga tcgagcgcgc ccggcaaaac cgcaatgcag
    22261 gtcgccgcac tattcttttt gttgacgaag ttcaccgttt caacaaaagc cagcaggatg
    22321 catttctgcc acatattgaa gacggcacca tcacttttat tggcgcaacc actgaaaacc
    22381 cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg tgtttatctg ttgaaatccc
    22441 tgagtacaga ggatattgag caagtactaa ctcaggcgat ggaagacaaa acccgtggct
    22501 atggtggtca ggatattgtt ctgccagatg atacacgacg tgccattgca gaactggtga
    22561 atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat ggcggatatg gccgaagtct
    22621 atgatagcgg taagcgggtc ctgaagcctg aattactgac cgaaatcgcc ggtgaacgta
    22681 gcgcccgctt tgataacaaa ggcgatcgct tttacgatct aatttccgca ctgcataagt
    22741 cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta tgcgcgaatt attaccgctg
    22801 gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat tgcgtctgaa gacgtcggta
    22861 atgccgatcc acgggcgatg caggtggcaa ttgcggcctg ggattgcttt actcgcgttg
    22921 gcccggcgga aggtgaacgc gccattgctc aggcgattgt ttacctggcc tgcgcgccaa
    22981 aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc cgatgctcgc gaacgcccgg
    23041 attatgacgt gccggttcat ttgcgtaatg cgccgacgaa attaatgaag gaaatgggct
    23101 acgggcagga atatcgttac gctcatgatg aagcaaacgc ttatgctgcc ggtgaggttt
    23161 acttcccgcc ggaaatagca caaacacgct attatttccc gacaaacagg ggccttgaag
    23221 gcaagattgg tgaaaagctc gcctggctgg ctgaacagga tcaaaatagc cccataaaac
    23281 gctaccgtta atgttatcgt tgcggtaatg ttgttactgt atccctgtgg tcgcaggctg
    23341 tggccacatc tcccatttaa ttcgataagc acaggataag catgctcgat cccaatctgc
    23401 tgcgtaatga gccagacgca gtcgctgaaa aactggcacg ccggggcttt aagctggatg
    23461 tagataagct gggcgctctt gaagagcgtc gtaaagtatt gcaggtcaaa acggaaaacc
    23521 tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca ggcgaaagcg cgcggggaag
    23581 atatcgagcc tttacgtctg gaagtgaaca aactgggcga agagctggat gcagcaaaag
    23641 ccgagctgga tgctttacag gctgaaattc gcgatatcgc gctgaccatc cctaacctgc
    23701 ctgcagatga agtgccggta ggtaaagacg aaaatgacaa cgttgaagtc agccgctggg
    23761 gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt gacgctgggt gaaatgcact
    23821 ctggcctcga ctttgcagct gcagttaagc tgactggttc ccgctttgtg gtaatgaaag
    23881 ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat gctggatctg cataccgaac
    23941 agcatggcta cagtgagaac tatgttccgt acctggttaa ccaggacacg ctgtacggta
    24001 cgggtcagct gccgaaattt gctggcgatc tgttccatac tcgtccgctg gaagaagaag
    24061 cagacaccag taactatgcg ctgatcccaa cggcagaagt tccgctgact aacctggtgc
    24121 gcggtgaaat catcgatgaa gatgatctgc caattaagat gaccgcccac accccatgct
    24181 tccgttctga agccggttca tatggtcgtg acacccgtgg tctgatccgt atgcaccagt
    24241 tcgacaaagt tgaaatggtg cagatcgtgc gcccagaaga ctcaatggcg gcgctggaag
    24301 agatgactgg tcatgcagaa aaagtcctgc agttgctggg cctgccgtac cgtaaaatca
    24361 tcctttgcac tggcgacatg ggctttggcg cttgcaaaac ttacgacctg gaagtatgga
    24421 tcccggcaca gaacacctac cgtgagatct cttcctgctc caacgtttgg gatttccagg
    24481 cacgtcgtat gcaggcacgt tgccgcagca agtcggacaa gaaaacccgt ctggttcata
    24541 ccctgaacgg ttctggtctg gctgttggtc gtacgctggt tgcagtaatg gaaaactatc
    24601 agcaggctga tggtcgtatt gaagtaccag aagttctgcg tccgtatatg aacggactgg
    24661 aatatattgg ctaataccca atttttctga atctaaaaag cgcctttagg gcgctttttt
    24721 tgtctccctt tgataccgaa caataattac tcctcactta cacgtaatac tactttcgag
    24781 tgaaaatcta cctatctctt tgattttcaa attattctat gtatacaagc ctatatagcg
    24841 agctgctata gaaataatta cacaatacgg tttgttactg gaatcaatcg tgatcaagct
    24901 tgagtgagcc attatgaaaa cgaaaatccc tgatgcggta ttggctgctg aggagagtcg
    24961 ccgtggtttg gtaaaaacga cagcgatcgg cggcctggca atggccagca gcgcattaac
    25021 attacctttt agtcggattg cgcacgctgt cgatagcgcc attccaacaa aatcagacga
    25081 aaaggttatc tggagcgcct gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca
    25141 cgtcgtggac ggtgaaatca aatatgtcga aacggacaat accggcgatg acaattacga
    25201 cggcctacac taggttcgcg cctgcctgcg tgggcgttcc atgcgtcacc gtgtctacaa
    25261 tccggaccgc ctgaaatatc cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga
    25321 gcgcattagc tgggaagaag cctacgacat catcgcgacc aatatgcagc gcctgatcaa
    25381 agagtacggc aacgagtcta tctatctgaa ctatggcacc ggtacgctgg gcggcaccat
    25441 gacccgctcc tggccgccgg gaaataccct ggtcgcgcgc ctgatgaact gctgcggcgg
    25501 ttatcttaac cattacggtg actactcttc cgcgcaaatt gcggaaggtt tgaaccatac
    25561 ctacggcggc tgggcagatg gtaacagccc gtcggatatc gaaaacagta aattggtagt
    25621 gctgtttggt aataacccag gcgaaacgcg aatgagtggc ggtggggtga cttactatct
    25681 tgaacaggca cgccagaaat ctaatgctcg catgatcatc atcgatccgc gctataccga
    25741 caccggtgcc gggcgcgaag atgagtggat ccctattcgt ccaggaacag atgccgcact
    25801 ggttaacggt ctggcgtacg tcatgatcac taaaaacctg gtggatcagg cattcctcga
    25861 taaatattgc gttggctatg acgagaaaac cctgcccgcc agtgcgccga aaaatggtca
    25921 ttataaagct tatattctgg gtgaagggcc agatggcgtg gctaaaacac cggaatgggc
    25981 ctcgcaaatc accggtgttc cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag
    26041 taccaaaccg gcgtttatca gccagggatg gggcccgcag cgtcacgcta acggtgaaat
    26101 cgcaacccgt gctatctcga tgctggcgat tctgaccggt aacgttggta ttaatggagg
    26161 gaacagcggc gcgcgtgaag gttcatacag cttaccgttt gtccgtatgc cgaccttgga
    26221 aaacccgatc cagaccagca tttcgatgtt tatgtggacc gatgccattg aacgtggccc
    26281 ggaaatgacg gcgctgcgtg atggtgtgcg cgggaaagat aagctggatg tgccgatcaa
    26341 aatgatctgg aactatgccg gtaactgcct gattaaccag cattctgaaa tcaaccgtac
    26401 ccatgaaatc ctccaggatg ataagaagtg cgagctgatt gtggttatcg actgccacat
    26461 gacctcatcg gcgaaatatg ctgacatcct gctgcctgac tgcaccgctt ccgaacagat
    26521 ggactttgca ctggatgcat cctgcgggaa tatgtcttac gtgattttca acgatcaggt
    26581 gattaaaccg cgctttgaat gtaagaccat ctatgaaatg accagcgaac tggcaaaacg
    26641 tcttggcgtt gagcaacagt ttactgaagg ccgtacccag gaagagtgga tgcggcatct
    26701 gtatgcccag tcgcgggaag cgattcctga actgccaacg tttgaagagt tccgcaagca
    26761 ggggatcttt aaaaagcgcg acccacaagg gcatcacgtt gcttataaag ccttccgtga
    26821 agatccgcag gcaaacccac tgactacgcc atcgggcaaa attgagattt attcgcaggc
    26881 gctggctgac attgccgcta cctgggaatt gccagaaggc gatgtgatcg atccactgcc
    26941 gatctacacg ccgggctttg aaagttatca ggatccgctg aacaaacagt atccgctgca
    27001 gcttacaggt ttccactata aatctcgcgt tcactcaact tacggcaacg ttgatgtgct
    27061 gaaagcggct tgccgtcagg aaatgtggat caacccgctt gatgcccaaa aacgcggtat
    27121 ccacaacggc gataaagtca ggatctttaa cgatcgtggt gaggttcata ttgaggcgaa
    27181 agtgacgcca cgaatgatgc cgggcgtggt cgcactgggt gaaggcgcct ggtatgaccc
    27241 ggatgcaaaa cgtgtcgata agggcggttg tatcaacgta ctgaccactc aacgtccgtc
    27301 tcctctcgct aaggggaatc cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg
    27361 agtaaccgat gacaacccag tatggatttt ttattgattc cagccgttgc accggttgca
    27421 aaacctgcga gctggcttgt aaagactaca aagatttgac gccagaagtc agtttccgcc
    27481 gcatttatga atatgctggc ggcgactggc aggaagataa cggtgtctgg caccagaacg
    27541 tgtttgccta ctatctgtcg atttcgtgca accactgcga agatccggct tgtactaaag
    27601 tctgcccgag cggtgcgatg cataaacgcg aagatggttt tgttgtggtc gatgaagatg
    27661 tatgcattgg ctgccgttac tgtcatatgg cttgcccgta tggcgcaccg caatataacg
    27721 aaacgaaagg ccatatgacc aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa
    27781 agccgatctg tgttgaatct tgcccgctgc gggcgctgga tttcggccct atcgacgagc
    27841 tgcgtaaaaa acatggcgat ctggcggccg ttgcgccgtt gccgcgagct cactttacca
    27901 aaccgaatat tgtgatcaaa cccaatgcca atagccgccc gaccggggat accactggct
    27961 atctggcaaa cccgaaggag gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg
    28021 atcttcacgg tcttcgggca atgtgtggcg ggtggtttta tcgttctggc tttggcgctg
    28081 ctcaaaggcg acctgcgagc agaagcccag cagcgtgtta tcgcctgcat gtttggctta
    28141 tgggtactga tgggcattgg ctttatcgcc tctatgctcc atcttggttc accgatgcgc
    28201 gcctttaact cgctcaaccg ggtaggggct tcagcactca gtaacgaaat cgccagcggt
    28261 tcgatctttt ttgctgtagg cggcatcggc tggttgctgg caatgctgaa aaagctgtca
    28321 ccggcattgc gtacgctatg gctggtggtg acgatggttc ttggcgtcgt ctttgtctgg
    28381 atgatggtgc gtgtgtataa cagcattgat accgttccga cctggtacag catctggacg
    28441 ccgatgggct gggcgatgcg tctgctgcca gccatttctg tactggcact ggtggtgagt
    28501 ggcgtgatgt cagtgatgca gggcacagag ctggcaacta ttcatagttc tgtgcagcag
    28561 gccgcagcgc tggtgccgga ctatggtgcg ctgatgtcct ggcggatcgt gcttttggcc
    28621 gttgccctgt gcttgtggat tgcaccacag ctaaaaggtt atcagcctgc ggttccgcta
    28681 ctatccgtct cattcattct gcttctggca ggggaattaa tcggtcgcgg cgtattctat
    28741 ggtttgcata tgaccgtggg gatggccgtc gcaagctaat cagtaacaac cggggtttcg
    28801 gccccggttc tcttttattt ctgcttcgtt aacgtgtcat aactggtcat caaattacga
    28861 taatcaggaa tatggttaga gaacaacgtc gccagtcctt caatatcatt acgccagtca
    28921 cggtgcagct cgcaagccac gccgaaccat gtcatcaatt gcgcaccagc ttgcgacatg
    28981 cgatcccacg ccgaatgccg ggtaatttca ttaaaggtcc ctgatgcatc agtcacgaca
    29041 aagacatcaa acccttcttc aatggctgaa agcgccggga atgccacgca aacttcggtt
    29101 accacaccgg caataattaa ctgtttttta cctgtcgctt tgactgcttt tacaaaatct
    29161 tcgttatccc aggcgttaat attgcccggg cgagcaatgt aaggtgcatc ggggaattgt
    29221 gctttcagtt ctagaactaa tgggccgtta ggaccggttt caaaactggt ggtaagaata
    29281 gttggtaagt tgaaatactt agccaggtca cccagcgcca gcacgttatt tttaaactta
    29341 tcgggttcga tatcccgtac aagggaaagt aaaccagcct ggtgattaac aagcaaaacg
    29401 gcagcatcat ttttatcaag acgaacatac ggtttggtca tcctcttctc ctttcgagag
    29461 tcagtggtac gtctgaaatc ggtcagacgc gatgcattgc tctgaaagca tagacgggaa
    29521 atatgagttt gctgtgacca tgaaattttt cgactgaacg aagcgtatga aatttgtgtt
    29581 agatcaataa aaacaatcag atgagattaa tcatctcttc agcattagcg ttatgaatga
    29641 gtaatataaa tcgataatac cgccgctgcg atatgcgcgt attttcaccc attgacaatg
    29701 tttttggcgg tggcatgatg cgcatgaaat ttgaacttcc tcacggtttt aattcatgtc
    29761 cacgtatacc cggcctgtca tgcttttgct gtctggcctg cttttgttga ctctggcgat
    29821 cgcggtgtta aatacactcg tgccgctttg gctcgcccag gaacacatgt ccacatggca
    29881 ggtaggcgtt gtcagctcat cctattttac cggcaacctt gtcggtacat tgctgacagg
    29941 gtatgtcatt aaacgcattg gctttaaccg cagctattat ctgtcctcct tcatttttgc
    30001 tgctggctgt gccggccttg gcctgatgat tggtttctgg agctggttgg cgtggcgttt
    30061 tgtcgcgggc gtcggctgtg ccatgatttg ggtggttgtt gagagcgcgc tgatgtgcag
    30121 tgggacgtca cgtaaccgtg ggcgtttgct tgctgcgtat atgatggttt attacgtggg
    30181 aacgttttta ggccagttac tggtcagcaa agtctcaacc gagctgatga gcgtattgcc
    30241 gtgggttaca ggtttgacat tggcagggat cttaccgctg ttgtttacgc gtgtcctgaa
    30301 tcagcaggct gaaaaccatg attcgacgtc aattacggca atgctaaaac tccgtcaggc
    30361 gcggcttggc gtgaatggct gcattatctc aggaatcgtt ctgggatctc tatatggcct
    30421 gatgcctctg tacctcaatc acaaaggggt gagcaatgcc agtattggtt tctggatggc
    30481 ggtactggtc agtgcgggta tcctcggaca atggccgatt ggacgtctgg cggataagtt
    30541 tggtcgactg ctggttttgc gtgttcaggt ctttgtcgtc attctcggca gtattgcgat
    30601 gcttagccag gcggcgatgg ccccagcgtt attcatcctc ggtgccgctg gctttacgct
    30661 atatccggtg gcgatggcct gggcttgtga gaaagttgaa catcatcaac tggtggcgat
    30721 gaaccaagcc ttactgttga gctataccgt gggaagtctg cttggcccgt catttagcgc
    30781 tatgctaatg cagaatttct ccgataatct attgtttatc atgatcgcca gcgtatcgtt
    30841 tatctatttg ctgatgctgc ttcgcaacgc cggtcatacg ccgaaacccg ttgctcacgt
    30901 gtaaatgaat tcaagcaaag tgtgaactta ctgtttcaca ctctgctttt ttgtttcttc
    30961 tatctgactt gcttttttcc aaattttatt cgtttaaaaa taaaatgtgc agcaggttat
    31021 aattttgcat ttcgctattt ccgcacttct tatttgccgc gcataatccc tcgttttaca
    31081 gatgcccctt taattttggc gaaggatttg tctatggctg ggaatgttca g
//
LOCUS       DAJTRB010000044        30073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTRB010000044 DAJTRB010000000
VERSION     DAJTRB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.044"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..79
                     /locus_tag="OO330_002110"
     CDS             <1..79
                     /locus_tag="OO330_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5882971.1"
                     /translation="DDGLRFAIREGGRTVGAGVVAKVLS"
     gene            309..692
                     /gene="secE"
                     /locus_tag="OO330_002111"
     CDS             309..692
                     /gene="secE"
                     /locus_tag="OO330_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5882972.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            694..1239
                     /gene="nusG"
                     /locus_tag="OO330_002112"
     CDS             694..1239
                     /gene="nusG"
                     /locus_tag="OO330_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5882973.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1398..1826
                     /gene="rplK"
                     /locus_tag="OO330_002113"
     CDS             1398..1826
                     /gene="rplK"
                     /locus_tag="OO330_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5882974.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1830..2534
                     /gene="rplA"
                     /locus_tag="OO330_002114"
     CDS             1830..2534
                     /gene="rplA"
                     /locus_tag="OO330_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5882975.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2947..3444
                     /gene="rplJ"
                     /locus_tag="OO330_002115"
     CDS             2947..3444
                     /gene="rplJ"
                     /locus_tag="OO330_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5882976.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3511..3876
                     /gene="rplL"
                     /locus_tag="OO330_002116"
     CDS             3511..3876
                     /gene="rplL"
                     /locus_tag="OO330_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5882977.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4196..8224
                     /gene="rpoB"
                     /locus_tag="OO330_002117"
     CDS             4196..8224
                     /gene="rpoB"
                     /locus_tag="OO330_002117"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5882978.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8301..12524
                     /gene="rpoC"
                     /locus_tag="OO330_002118"
     CDS             8301..12524
                     /gene="rpoC"
                     /locus_tag="OO330_002118"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5882979.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADHI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12766..13899)
                     /gene="thiH"
                     /locus_tag="OO330_002119"
     CDS             complement(12766..13899)
                     /gene="thiH"
                     /locus_tag="OO330_002119"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5882980.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13896..14666)
                     /gene="thiG"
                     /locus_tag="OO330_002120"
     CDS             complement(13896..14666)
                     /gene="thiG"
                     /locus_tag="OO330_002120"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5882981.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14668..14868)
                     /gene="thiS"
                     /locus_tag="OO330_002121"
     CDS             complement(14668..14868)
                     /gene="thiS"
                     /locus_tag="OO330_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5882982.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14852..15607)
                     /gene="thiF"
                     /locus_tag="OO330_002122"
     CDS             complement(14852..15607)
                     /gene="thiF"
                     /locus_tag="OO330_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5882983.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15600..16235)
                     /gene="thiE"
                     /locus_tag="OO330_002123"
     CDS             complement(15600..16235)
                     /gene="thiE"
                     /locus_tag="OO330_002123"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5882984.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16235..18130)
                     /gene="thiC"
                     /locus_tag="OO330_002124"
     CDS             complement(16235..18130)
                     /gene="thiC"
                     /locus_tag="OO330_002124"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5882985.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18211..18311)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18364..18840)
                     /gene="rsd"
                     /locus_tag="OO330_002125"
     CDS             complement(18364..18840)
                     /gene="rsd"
                     /locus_tag="OO330_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5882986.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18935..19708
                     /gene="nudC"
                     /locus_tag="OO330_002126"
     CDS             18935..19708
                     /gene="nudC"
                     /locus_tag="OO330_002126"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5882987.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19748..20812
                     /gene="hemE"
                     /locus_tag="OO330_002127"
     CDS             19748..20812
                     /gene="hemE"
                     /locus_tag="OO330_002127"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5882988.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20969..21640
                     /gene="nfi"
                     /locus_tag="OO330_002128"
     CDS             20969..21640
                     /gene="nfi"
                     /locus_tag="OO330_002128"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5882989.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21683..22273
                     /locus_tag="OO330_002129"
     CDS             21683..22273
                     /locus_tag="OO330_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5882990.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22460..22732
                     /gene="hupA"
                     /locus_tag="OO330_002130"
     CDS             22460..22732
                     /gene="hupA"
                     /locus_tag="OO330_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5882991.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22745..23440
                     /locus_tag="OO330_002131"
     CDS             22745..23440
                     /locus_tag="OO330_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5882992.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23442..23867)
                     /gene="zraP"
                     /locus_tag="OO330_002132"
     CDS             complement(23442..23867)
                     /gene="zraP"
                     /locus_tag="OO330_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5882993.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24105..25481
                     /gene="zraS"
                     /locus_tag="OO330_002133"
     CDS             24105..25481
                     /gene="zraS"
                     /locus_tag="OO330_002133"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5882994.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25478..26803
                     /gene="zraR"
                     /locus_tag="OO330_002134"
     CDS             25478..26803
                     /gene="zraR"
                     /locus_tag="OO330_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5882995.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26800..28089)
                     /gene="purD"
                     /locus_tag="OO330_002135"
     CDS             complement(26800..28089)
                     /gene="purD"
                     /locus_tag="OO330_002135"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5882996.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28101..29690)
                     /gene="purH"
                     /locus_tag="OO330_002136"
     CDS             complement(28101..29690)
                     /gene="purH"
                     /locus_tag="OO330_002136"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5882997.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7038 a         7840 c         8277 g         6918 t
ORIGIN      
        1 ggacgacggt ctgcgtttcg caatccgtga aggcggccgt accgttggcg cgggcgttgt
       61 agcaaaagtt ctgagctaat tgccgataac atttgacgca atgcgcacta aaagggcatc
      121 atttgatgcc ctttttgcac gctttcatac cagaacctgg ctcatcagtg attttctttg
      181 tcataatcat tgctgagaca ggctctgttg agggcgtata atccgaaaag ctaatacgcg
      241 tttcgatttg gtttgcctcg cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag
      301 gttggtttat gagtgcgaat accgaagctc aaggaagcgg gcgcggcctg gaagcgatga
      361 agtgggtcgt tgtggtggca ttgctcctgg cggcgattgt cggcaactac ctttatcgcg
      421 acattatgct gccgctgcgt gcgctggccg tagtaattct gattgctgca gcgggtggtg
      481 tcgcgctgtt aacgacaaaa ggtaaagcta ccattgcttt tgcccgtgaa gcgcgtaccg
      541 aagtccgtaa ggtcatttgg ccgactcgcc aggaaacatt gcacaccacg ctgattgtgg
      601 ctgcggttac cgcagtaatg tcactgatcc tgtggggact ggatggtatt ctggttcgcc
      661 tggtatcctt tatcactggc ctgaggttct gagatgtctg aagctcctaa aaagcgctgg
      721 tacgtcgttc aggcgttttc cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat
      781 atcaaattac acaacatgga agatttgttt ggtgaagtca tggtaccaac cgaagaagtg
      841 gttgaaatcc gtggcggtca gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc
      901 ctcgttcaga tggtgatgaa cgacgcgagc tggcacttgg tgcgcagcgt accgcgtgtg
      961 atgggcttca tcggcggtac ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat
     1021 gcgattatga accgcctgca gcaggttggt gataagccgc gtccgaaaac gctgtttgaa
     1081 ccgggtgaaa tggtccgtgt taatgatggt ccgttcgctg acttcaacgg tgttgttgaa
     1141 gaagtggatt acgagaaatc tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc
     1201 ccggtagagc tggacttcag ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc
     1261 gatttaatcg ttgcacaagg cgtgagattg gaatacaatt tcgcgccttt tgtttttatg
     1321 ggccttgccc gtaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1381 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1441 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1501 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1561 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1621 cggcagcagt tctgctgaaa aaagcggcag gtatcaagtc tggttccggt aagccgaaca
     1681 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1741 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1801 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1861 tccgtgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1921 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1981 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     2041 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2101 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2161 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2221 tgggtccgcg tggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2281 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2341 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2401 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2461 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2521 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2581 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2641 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2701 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2761 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2821 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2881 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2941 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     3001 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3061 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3121 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3181 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3241 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3301 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3361 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3421 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3481 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3541 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3601 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3661 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3721 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3781 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3841 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3901 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3961 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     4021 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4081 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4141 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4201 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4261 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4321 tcctgaaggg cagtatggtc tggaagctgc tttccgttcc gtattcccga ttcagagcta
     4381 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4441 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4501 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4561 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4621 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4681 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4741 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4801 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4861 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4921 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4981 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     5041 caaactgatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5101 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5161 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5221 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5281 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5341 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5401 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta ttctgagcaa
     5461 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5521 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5581 ccagttccgc gttggcctgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5641 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5701 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5761 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5821 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5881 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5941 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     6001 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6061 cctggatgaa gaaggccact tcgtagaaga cttggtaact tgccgtagca aaggcgaatc
     6121 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6181 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6241 tgcgaatatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6301 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6361 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6421 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6481 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6541 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6601 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6661 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6721 caccaagctg ggtccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6781 ctccaaactg gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6841 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6901 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6961 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     7021 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7081 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7141 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7201 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7261 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7321 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7381 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7441 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7501 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7561 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7621 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7681 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7741 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7801 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7861 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7921 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7981 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     8041 cgcagcatac accctgcagg aaatgctcac tgttaagtct gatgacgtga acggtcgtac
     8101 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8161 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8221 gtaattctcg ctcaaacagg tcactgctgt cggggtaaaa cccggcagcg gattgtgcta
     8281 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8341 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8401 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8461 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8521 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8581 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8641 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8701 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8761 tggaacgtca gcagatcctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8821 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8881 tggagcaaga gtgcgaacag ctgcgtgaag agctgaacga aaccaactcc gaaaccaagc
     8941 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     9001 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9061 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9121 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9181 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9241 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9301 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9361 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9421 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9481 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9541 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9601 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9661 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9721 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9781 cgaacggcga accaatcatc gttccgtctc aggacgttgt actgggtctg tactacatga
     9841 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9901 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9961 agtatgaaaa agatgctaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
    10021 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10081 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10141 tgaaaccgac cgttattttt gcggaccaca tcatgtacac cggcttcgcc tatgcagcgc
    10201 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10261 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10321 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10381 ccaaagcgat gatggataac ctgcaaactg aaaccgttat taaccgtgac ggtcaggaag
    10441 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10501 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcaaagccg gatggctcca
    10561 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10621 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcgaactccg
    10681 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10741 gtggtaccca tgaaggtatc atgatgactc cggttatcga ggggggtgac gttaaagagc
    10801 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10861 ctgatattct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10921 agaactctgt cgacgcggta aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10981 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aaaggtgaag
    11041 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11101 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11161 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11221 ttatcacttc ccgtaacacc gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11281 gctacaaagt gccttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggcg
    11341 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11401 ttgtacgctt tactgacatg atcgacggcc agaccattac tcgtcagacc gacgaactga
    11461 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11521 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatc ccaggtaccg
    11581 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11641 agatcagctc tggtgacacc ttggcgcgta ttccgcagga atccggcggt accaaggaca
    11701 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc tcgtcgtccg aaagagccgg
    11761 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11821 gtctggttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11881 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11941 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgtgact cgttacatcg
    12001 ttaacgaagt acaggacgta taccgtctgc agggcgtaaa gattaacgat aaacacatcg
    12061 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12121 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12181 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12241 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12301 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12361 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12421 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12481 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12541 aacgtaaaaa cccgcttcgg cgggtttttt tatgggtgtt attcagaggg caaattcttc
    12601 acggaaaata ttaagaaagg cagggcgaaa cgaggaagaa gcttttcgcc ctggacaaca
    12661 ttcctgctga cggcactacc ataaaaaaac acaacgcttt caacaacctt gcgtttttca
    12721 tcttccgtag cggctcggaa taaatttacg ttcttacatt ccgtctcata gtctttgcga
    12781 ggcgcgtccc agatagctgt cccagtcttt ccataccggc tgcaaaccct gagcggttaa
    12841 cacggcagca accgcttccg gtctgcgatc gtcgtgcggt gagaactgtt ccaactcggg
    12901 gtgattatcg gcatagccac ctggctgcgt tttcgagaag gcgctgacgt tattgatcgc
    12961 cagcggaata acgcgatcgc gaaaccacgg tgattcccgc gtggagagtg acagttcaat
    13021 ctccggtgca agcagtcgga aggcgcagat ggtttgcact aactggcgtt catccataat
    13081 cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc ggaaaggaga cagagtaacg
    13141 gctttgccag taatgctgtt gcagccatag caaatgttcg gcaaccatat agcagtcaac
    13201 gcgccagttg tcggaaaggc caattagcgc gccgaggcct atcttatcaa tccccgcacg
    13261 ccccagccga tccggcgttt ccagccgcca gaagaagtcc tgttttttgc ctttcaaatg
    13321 atggcgggta taagtcgcct cgtgatatgt ctcctgataa accatcaccc catccagacc
    13381 aagttgcttt aactcggcgt attccgtctc cgccagcggt tgcacttcca tttgtagtga
    13441 agagaactgt tcacgcaggg cggggagatg acgacgaaag taatccatcc ccactttcgc
    13501 ctgatgttca ccagtgacta atagcagatg ttcaaagccc atctcccgta tagcggcgct
    13561 ttccctggca atatccgctt catccagcgt tttgcgcttg atgcgattac tcatggaaaa
    13621 tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga taaagcggga cgtagaaact
    13681 aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc tgttgggcca gttgttccag
    13741 atagccactg gcggcaggcg ataacagcgc catcatgtcg tcgcgggtga gttgcgaggc
    13801 atttagcgcc cgctctacgt cagcagccgt tttgccgttg atacgcaggc ggatgtcgtc
    13861 ccagtccagt tgtcgccagc gattgctgaa ggttttcatg ccgatgcctc cagaaatccg
    13921 gtcagcgggc tggtggcatg agcaaaataa ctgcggctgc ccggtccgga ctgacgtgcc
    13981 agcaggccta cttctaccgc cagacgaaat gccttcgcca tgttgacggg atcgtccgcg
    14041 acggcaatcg ccgtattcac taacaccgcg tcggccccca tttccagcgc ctgcgtggca
    14101 tggctgggaa cgccgatgcc agcatcgaca accaccggaa ctgtggcctg ctggataata
    14161 atctccagca tggcacgggt ttccagtccc tgattcgagc caatcggcgc gccgagcggc
    14221 atcaccgctg cacagccgac ttcttccaga cgtttacaca ataccggatc ggccccgcag
    14281 taaggcagca cgacaaatcc ctgttgtacc agcgtttcgg cggctttcag ggtttcgatg
    14341 ggatcgggca acagccagcg ggcgtcaggg tgaatctcca attttagcca gtttgtgcct
    14401 aacgcttcac gtgccagatg ggcggcgaaa atggcttctt ccgctgtttt cgccccggaa
    14461 gtattcggca gcagggccac acccgccgcg ataagcggtt cgaggatagc gtcgttgtgc
    14521 tggcgcaagt cgacacgttt catcgccagt gtcaccagct ggctgccgga agcgcggatc
    14581 gcctccacca tcagttgtga agaagcgaat ttccctgtgc cggtaaacag atgtgaatca
    14641 aacgttttgt ccgcaatacg taacatctca accccctgca ataacctgaa aaagcaggat
    14701 ctggtcgcca tcctgcacga tatgttgcgt ccactgctca cgcgggacga tttgctgatt
    14761 aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc tccagtagtt cgtgaacagt
    14821 ttgcccggcg gcgcactgca tcggttgatc gttaaacagg atctgcattg cttcctccgc
    14881 ataccgggca accactggcg cggcgcaacg ccaggctgcg ccactggctc gacttaccgt
    14941 cgaacagtcg gagttctccc gcaggcgtct ctataccgct taataactta atggcttcca
    15001 gtgcctgcaa agtgcccatc accccgacca ccgggccaac cacgcccgcc gtgcggcagt
    15061 tgcgttctgg ctgctggtta tctggccaca ggcagcggta acacccctgc tcccagggcg
    15121 gtgtcagtac catcaactga ccgccaaatc cgaccgcgct ggcggtgata agcggcgtgt
    15181 tgagtgccac gcaggcggca ttaatctcct ggcgagtcgc catattgtcg gtacagtcga
    15241 gcaccacatc ggcttgtgca accgcatctt tcaacgcctc acccgttaac cgttgttgta
    15301 atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg ctgttggctg acctgcgatt
    15361 tcgggctgtt gatatcttca gtggtaaaga ggatttgtcg ttgcagattg cttaaatgca
    15421 catcgtcgtc atctgccagt accagcgtcc cgacgccagc ccctgccagg tatagcgcag
    15481 caggtgtacc cagcccgcca agaccaataa tcagcacctg gctgtcgagc agtttttgct
    15541 gcccgtcaag agcgatatcg tcgagcagaa tttggcggct ataacgcata aagtcacggt
    15601 cattcatcgc caactcctgc aatttccagc aactgtgccg ttgccaaacg ccagtctgcg
    15661 gcttgagtaa tggcgctgac gacggcgata ctgccgacac ccgttgctat caccgcaggc
    15721 gcgcgtgcca gactgatacc gccaatcgcc acggtgggat aatccgccag gcgctcaaca
    15781 tgccgtgcca actgttccag cccctgcggt gcagaaggca tctgtttggt ttgcgtcggg
    15841 aacacatgtc ccagcgcgat ataagagggg cgtgctgcca gcgcgacgtc gatttccata
    15901 tcgtcatgtg tcgaaacgcc cagccgcagg cctgccgcgc ggatggcatt gagatcggtg
    15961 gcttgcaaat cttcctgccc caaatggacg ccatacgcct gatgcttgat cgcgagccgc
    16021 cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc ccagcgcaat tgccgccacg
    16081 acatcggctt ccacctcttc atcgcgccga tctttgatgc gtagctggag agtacgtacg
    16141 cctgcatcca acagacgttc gatccactgt acgctgtcca ccaccgggta cagtcctaaa
    16201 cgaaaaggta caggaggaaa atcaggctga tacatcacgc ttcctcctta tgcaggtaga
    16261 tttctccgcc tctggcacgg aagttctccg acatatccgc cattcccact tcaatggttt
    16321 gcgcggcggc gtaatcgcgt acttcctggc tgattttcat cgagcagaat tttggcccac
    16381 acatggagca aaaatgggcg actttgccgg actcttgggg cagggtttca tcgtgataag
    16441 cgcgggcggt aaacgggtcg agggccagat taaactggtc ttcccagcga aattcgaagc
    16501 gggctttcga catggcgtta tcgcgaattt gcgcgcccgg atgccctttt gccaggtcag
    16561 cagcgtgggc ggcaatctta taagtgataa gcccctgctt aacatcttct ttattgggca
    16621 gacccagatg ctcttttggc gttacgtaac agagcatcgc gcagccaaac cagccaatca
    16681 tcgccgcacc aatccccgac gtgaagtggt catagcccgg tgcaatatcg gtagttagcg
    16741 gccccagagt gtaaaacggc gcttcgtggc agtgctctaa ctcctcggtc atattgcggc
    16801 ggatcatctg catcggcaca tggcctgggc cttcaatcat cacctgtacg tcatattccc
    16861 aggcgatttt ggtcagttcg cccagcgtat gcaattcggc aaactgcgct tcatcgttgg
    16921 cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga cagcgaaacg tcataagcgg
    16981 cacagatttc acaaatttcg cggaagtgtt gatagaggaa attttcctga tgatgggaga
    17041 ggcaccattt cgccataatc gaaccgccgc gagagacgat accggtcagg cgtttcgcgg
    17101 tcatcggcac atagcgcagc agtacgcccg catggatagt gaagtaatcc acaccttgct
    17161 cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt aagatcttcg gcgatcccgt
    17221 taaccttctc cagcgcctgg tagatcggca ctgtaccgat cggcaccgga ctgttacgca
    17281 aaatccactc gcgggtttcg tgaatatagc gaccggtgga gagatccatc accgtatccg
    17341 ctccccagcg cgtggaccat accagctttt ccacttcttc ttcgatggaa gaggtgaccg
    17401 ccgagttgcc gatattggcg ttaactttta ccaggaagtt acggccaata atcatcggct
    17461 ccgattccgg atgattaatg ttggccggga taatcgcacg tccggcagca acttcatcac
    17521 ggacaaattc cgcagtgata ttttccggca gatgtgcgcc aaagctcatt cccggatgct
    17581 ggtggcgtaa aacctcgcta cggatgcgct cgcggcccat attctcgcgg atggcgatga
    17641 attccatgtc cggcgtgatg atgccctggc gggcgtagtg cagttgggtg acacggcgtc
    17701 ctgttttggc gcgttttggc gttaataagc cgctaaaacg tagctcgtcg agaccatcat
    17761 ctgccagccg cgttctggtg taatcggaac tgcgcacggt aagttcttcg gtatcggcgc
    17821 gcgcatcgat ccacggctgg cgtagttttg ccagcccttg ctgcacgtta atggcgatct
    17881 gcggatcacc atacgggccg gaggtgtcgt agaccggaat cgcttcgttt tcttcgtact
    17941 gcggctgttc tttgctaccg ccaattagcg tcgggctaag ctggatctca cgcatcggca
    18001 cgcgcacgcc ggggtgtgtg ccagtgatat aaatgcgttt tgagttggga aaggcggtgc
    18061 cttccagggt gtcgataaaa tgttgggccc gggcgcgttg ttcgcggcgg gtcagttttg
    18121 ttgcagacat agctcattcc aaaaagttaa ggacgtggct tgtcagacga cggatgaagc
    18181 aagagacgat cgccgcaaag gcgatgcgat agcagattaa ctcttgttcc cttcgcaggt
    18241 attagcctga tcaggttccg cggatcccga ataaacggtc tcagccagtt aaggcactcc
    18301 gacaagaaat tagcccccac aaacggggcg ttgaatgtaa attacgcgtt aacagcgcag
    18361 aactcaagca ggatgtttga cgcgggcggc gtcaagcacc agcagaatca gcttatcttc
    18421 cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat aaaacctgtt gaaactcaag
    18481 gtaattatca tgatcgatgg cggtttccag gctggaatcg tagtaatcca taatctgttg
    18541 ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc gcgagttgcc cgttgccttc
    18601 cagcttatga agaatgcgct cataaatact gaaatgtccg gcagacaagt aatcgaccag
    18661 gctctgacaa aaatcatcaa gggctttttc gtttagcctc atgtacgatt ctttgccagg
    18721 cttaatgcca accagattgt agtaagccac gagcagatgc ttacgtacat gtagccagcg
    18781 atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg ttatcgagct ggttaagcat
    18841 gattgactcc gcaagtttgt attcaaaaac tgctcagtga gaaatgtaaa aaccatgtta
    18901 aacatgccag tgatgcaaag gtagtgcaag agctatggat cgtataattg aaaaattaga
    18961 tcacggctgg tgggtcgtca gccatgaaca aaaattatgg ttgccgaagg gagaattgcc
    19021 atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc gcactacaaa tcggcgaatg
    19081 gcagggggaa cctgtttggt taatacaaca gcagcggcgt cacgatatgg ggtcggtacg
    19141 tcaggtcatt gatctcgatg ttgggctgtt tcaactggcc ggacgtggcg tgcaactggc
    19201 ggagttttac cgatcgcata aatactgtgg ttactgcggg catgaaatgt atccgagcaa
    19261 aaccgaatgg gcgatgctgt gtagccattg ccgtgagcgt tactacccgc aaatcgcccc
    19321 ctgcattatt gttgccatcc gtcgcgatga ttcgctcctc ctcgcccagc atacccgcca
    19381 tcgtaacggt gtccatacag tacttgccgg attcgtcgaa gtgggcgaaa ccctcgagca
    19441 ggcagtcgcg cgggaagtga tggaagagag cggaattaaa gttaaaaact tgcgttacgt
    19501 gacttctcag ccgtggccgt ttcctcagtc tttaatgacc gcgtttatgg cggaatatga
    19561 cagcggcgac atcgtgatcg acccgaaaga attgctcgag gcgaactggt atcgctatga
    19621 cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc cgtctgatag aagatactgt
    19681 ggcgatgtgt cgggcagagt atgagtgatg atacactgac cgcctgacgc actaaggaac
    19741 agccaaaatg accgaactta aaaacgatcg ttatctgcgg gcgctgctgc gccagcccgt
    19801 tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc tatctaccgg aatataaagc
    19861 cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa aacgccgagc tggcgtgcga
    19921 agtgactttg caaccgctgc gtcgctaccc gctggatgcg gcgatcctct tttccgatat
    19981 cctcaccgtg ccggacgcga tggggttagg gctctatttt gaagccggag aaggtccgcg
    20041 ttttacctcg ccagtcacct gcaaagccga cgtcgataaa ctgccaattc cggacccgga
    20101 agatgagctg ggttacgtga tgaacgcggt gcgtaccatt cgtcacgaac tgaaaggcga
    20161 agtgccgctg attggttttt ccggcagccc gtggacgctg gcaacctaca tggtggaagg
    20221 cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg tatgccgatc cgcaggcgct
    20281 gcacgctctg ctcgataaac tggcgaaaag cgtcactttg tatctgaatg cgcagattaa
    20341 agccggtgct caggcagtga tgattttcga cacctggggc ggcgtgctta ccgggcgcga
    20401 ttatcaacag ttctcgctct attacatgca taaaattgtt gatggtttac tgcgtgaaaa
    20461 cgacggtcgc cgcgtgccag tcacgctgtt taccaaaggc ggcggacagt ggctggaagc
    20521 catggctgaa accggttgcg atgcgctggg cctcgactgg acaacggaca tcgccgatgc
    20581 gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat atggatccgt cgatgctgta
    20641 cgcgccgcct gcccgcattg aagaagaagt agcgactata cttgcaggtt tcggtcacgg
    20701 cgaaggtcat gtctttaacc ttggtcacgg cattcatcag gatgtgccgc cagaacatgc
    20761 tggcgtgttc gtggaggcag tgcatcgact gtctgaacag tatcaccgct aagagcctga
    20821 tccatcaggc tattttattt gccattttga ggccgggcag tgctcaaaat cctcacgtac
    20881 tacgtgtacg ctccggtttt tccgcgctgt ccggcctcaa actgccttca acaattacgc
    20941 ctgttgaacc aggctctaag gagcgattat ggatctcgcg tcattacgcg ctcaacaaat
    21001 cgaactggct tcttctgtga tctgcgagga tcgactcgat aaagatccac cggatctgat
    21061 cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct
    21121 gctgaaatat ccctcgcttg agctggtgga gtataaagtt gcccgcatcg ccaccaccat
    21181 gccttacatt ccaggttttc tttccttccg cgaatatcct gcgctgctgg cagcgtggga
    21241 gatgctgtcg caaaagccgg atttagtgtt tgtcgatggt catgggatct cgcatcctcg
    21301 ccgtcttggc gtcgccagcc attttggctt attggtggat gtgccgacca ttggcgtggc
    21361 gaaaaaacgg ctctgcggta aattcgaacc gctctccagc aaaccgggcg cgctggcccc
    21421 actgatggat aaaggcgagc agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc
    21481 gttgtttatc gctaccggcc atcgggtcag cgtggacagc gcgctggcgt gggtacaacg
    21541 ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga
    21601 acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa
    21661 tttccgtatt tgagaactca acatgttaca aaacccaatt catctgcgtc tggagcgcct
    21721 ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc gaacgcatgt accccaatta
    21781 cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa atttaccgtc gtattctcga
    21841 tctcatctgg gaaacgctga ccgttaaaga cgcaaaagta aatttcgaca gccaactgga
    21901 gaaatttgaa gaagcgattc cttcagccga cgatttcgat ctgtacggcg tttatccggc
    21961 aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg cgtttgagtg gtgaaacgct
    22021 cgaacacgcg gtggaagtga gtaagacctc catcacgacc gttgcgatgc tggaaatgac
    22081 tcaggctggt cgcgaaatgg gcgatgaaga gcttaaagaa aacccagctg tagagcaaga
    22141 atgggacatt cagtgggaaa tattccgact tttagctgag tgcgaagaac gcgatatcga
    22201 gctgataaaa ggccttaggg cagacctgcg tgagtcgggt gagagcaata ttggtataat
    22261 ttttcagcaa taagaccaga aaacgtgatt taacgcctga tttgtcgtac ctggagtctt
    22321 ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa aaaagtggct atcggtgcgt
    22381 gtatgcagga gagtgctatt ctggcatttc cgtcgcactc gatgcttagc aagcgataaa
    22441 cacattgtaa ggataactta tgaacaagac tcaactgatt gatgtaattg cagagaaagc
    22501 agaactgtcc aaaacccagg ctaaagctgc tctggagtcc actctggctg caattactga
    22561 gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc ggtaccttca aagtgaacca
    22621 tcgcgctgag cgtactggcc gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc
    22681 taacgtaccg gcatttgttt ctggcaaggc actgaaagac gcagttaagt aagattgcgt
    22741 ggcagtgaac agttttaacg aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc
    22801 attgatgctg gcaggtgcgc tgtttctcac tgcctgtagc cacaactctt cacttcctcc
    22861 ctttaccgcc agtggatttg ctgaagacca gggcgcggta cgcatctggc gaaaagacag
    22921 cggcgataat gtgcatctgc ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac
    22981 gcgagagtat cgctggcagg gcgataacct cacgctcatc aatatcaatg tttacagcaa
    23041 accgccggtg aacattcgcg cgcgttttga cgatcgcggc gatctgagct ttatgcaacg
    23101 tgaatccgat ggggaaaagc agcagctttc taacgaccaa atcgatttat accgttatcg
    23161 tgctgatcag atccgccaga ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca
    23221 ggggcgctgg catgcgatgg aacagaccgt gaccacctgc gaagggcaaa ccattaaacc
    23281 tgatttagat tcgcaggcga tagcgcatat cgagcgccgc cagagccgct cttctgttga
    23341 tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa ttactgttag tggcaaactc
    23401 tgatttctgt cgctggcaac ccaacgagaa aacgttctga tttaccagtg gcccataccc
    23461 atatgaccgc caccaccgca gccgccgtag cccatgccca ttccggctcc gcgcggaata
    23521 cccgcttcag ccatcgcgat atctcgtttc acccgtaact catctaacga ctgacgcaaa
    23581 ttctccatct ctttggcgac cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac
    23641 agggcattgt attcataacg cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg
    23701 taaaagtcat tatggatttt ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca
    23761 ttttgctgcc acataccgtg tccgccgtga gcaaatgcag atgtcgatcc catcgccatt
    23821 gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc gtttcatggt taatcctcca
    23881 gtggttgtct tacttcgggt attgcatctt ccgtgccaac gatgaaacgc tgatatgacg
    23941 ggtaatctgg catgataaac gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt
    24001 ttactcattg aaacttaagc ctttgtgtta cagcgcaggg taagcgctga taaaagatgg
    24061 catgatttct gctgtcagaa agggatgagc aggcaaagaa gaagatgcgt tttatgcaac
    24121 gttctaaaga ctccttagct aaatggttaa gcgcgatcct ccccgtggtc attgttgggc
    24181 tggtagggtt gtttgcggtg acggtgattc gcgattatgg gcgcgagact gccgccgcca
    24241 gacaaacgct gctggaaaaa ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg
    24301 tcggcatggg gatgcgcatg catcatgcgc agcagcaggc cttactggaa gaaatggccg
    24361 ggcagcctgg agtacgttgg tttgcggtca cggatgaaca aggaacaatc gtgatgcata
    24421 gcaactccgg catggtggga aaacagcttt attcccctca ggaaatgcag cagttacatc
    24481 cgggagatga agaagcgtgg cggcggatcg atagcgcaga cggcgagcct gttctggaaa
    24541 tttatcgcca gtttcaaccg atgtttgctg ctggaatgta ccggatgcgc catatgcagc
    24601 agtatgccgc gacaccacaa gcaattttca tcgctttcga cgccagtaat attgtgagtg
    24661 ccgaagatcg tgagcagaga aacaccctga ttattctctt cgccctggcg acggtcttgc
    24721 tggcaagcgt attgtcattc ttctggtatc gccgctatct gcgctcgcgc cagcttctac
    24781 aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg gcatcttgcg gcaggcgttg
    24841 cccacgaaat ccgtaaccca ctttcctcga ttaaaggact ggcgaaatac tttgccgagc
    24901 gcgcgcctgc agggggagaa gcgcatcaac tggcgcaggt gatggcgaaa gaggccgacc
    24961 gtttaaaccg cgtggtaagc gagttgctgg aactggttaa gccaacgcat ctggctttgc
    25021 aggcggtgga tctcaacacg ctgattaacc actcattaca gctggtaagc caggatgcaa
    25081 acagccggga gatccagtta cgctttaccg ccaacgacac attaccggaa attcaggccg
    25141 atccggacag gctgactcag gtcctgttga atctctatct caatgctatt caggcgattg
    25201 gtcagcatgg cgtgattagc gtgacggcca gcgaaagcgg cgcgggcgtg aaaatcagcg
    25261 ttaccgacag cggtaaggga attgcggcag atcagcttga agccatcttc actccgtact
    25321 tcaccaccaa agccgaaggc accggattgg ggctggcggt cgtgcataat attgttgaac
    25381 aacacggtgg tacaattcag gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct
    25441 ggcttccggt caatattacg cgtaaggacc cacaaggatg acgcacgata atatcgatat
    25501 tctggtggtg gatgatgaca ttagccactg cactattttg caggctttac tgcgcggctg
    25561 gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg ctggagcagg tgcgggaaca
    25621 ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag atggacggca tcgccacgct
    25681 gaaagagatc aaagcgttaa acccggcaat tccggtgctg attatgactg cgtactccag
    25741 cgtcgagacg gcggtagagg cactgaaaac tggggcgcag gattatctca tcaagccgct
    25801 ggatttcgat aacctacagg cgacgctgga aaaggcgctc gcgcatacgc acagtattga
    25861 tgctgaaaca cctgcggtga ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat
    25921 gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca
    25981 cggcgattcc ggcaccggta aagagctggt cgccagggcg attcacgcca gtagcgcacg
    26041 tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc aacgaatcct tgctggaatc
    26101 tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc gataaacgcc gggaagggcg
    26161 ctttgttgag gcggacggcg gcacgctgtt tctcgatgaa attggcgata tctcgccgat
    26221 gatgcaggtg cgtctgctgc gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa
    26281 ccagactatc tcggttgatg tccggctgat tgcggcgacc catcgcgatc ttgccgcaga
    26341 ggtgaatgcc gggcgttttc gccaggatct ctactatcgc ctgaatgtgg tggcgattga
    26401 agtaccatcg ctgcggcaac ggcgggaaga tatccctctg ctggctggcc attttcttca
    26461 gcgctttgcc gagcgtaatc gcaaggcggt aaaaggcttt acgccacagg cgatggattt
    26521 gctgatccac tacgactggc cgggaaatat tcgtgaactg gaaaacgcgg tggaacgggc
    26581 ggtggtgctg ctgaccgggg aatatatttc cgaacgcgag ctgccgctgg cgattgccag
    26641 tacgccgatc ccgctggggc aaagtcagga tattcagccg ctggtggaag tggaaaaaga
    26701 ggtgattctg gcggcgctgg agaaaacggg cggcaacaaa accgaagccg cccgtcagtt
    26761 agggatcacg cgcaaaacgc tattggcaaa actgtcgcgt tagttctgct cgcgttcgat
    26821 ggcgcgccaa ccgatatctt tccggcagaa gcaatcatcc cagtggatat cggtcattaa
    26881 ggcgtaggcg cgtttctgcg cttctgccac ggtatgaccc agcgcggtga cgcacagtac
    26941 gcgcccgccg ctggttacca cctgctcgtc atccgccagt tttgtgcccg cgtggaacac
    27001 tttgccgtca gccacttctt ccagcggcag gccgtggatc acatcgccgg tgcggtaatc
    27061 gcccggatat ccacccgcag ccatcaccac gctgagagaa gcacgttcat cccactctga
    27121 cgttttctcg tccagtttgc cttcacaggc cgccaggcag agctcaacca gatcggactt
    27181 catgcgcagc ataatcggct gggtttccgg atcgccaaag cggcagttaa attcaataac
    27241 cttcggattg ccctgtttgt cgatcatcag gcccgcatag agaaaaccgg tgtaggtgtt
    27301 gccttccgcc gccatgcctt tcacggttgg ccagatgata cgttccatgg tgcgctgatg
    27361 aacttcatcg gttactaccg gcgcggggga gtaagcgccc atcccgccgg tgtttggtcc
    27421 gttatcttta tcgcctacgc gtttgtgatc ctggctggta gccatcggca gcacatgctc
    27481 gccgtccacc atcacgataa agctcgcttc ttcgccatcg aggaactctt cgataacgat
    27541 gcgatgaccc gcgtcgccaa aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc
    27601 ttcttccagc gtcatcgcca cgataacgcc tttcccggca gccagaccgt ccgctttaat
    27661 gacgattggc gcgcctttct cacgcagata caccagcgca ggttctacct cggtgaagtt
    27721 ctggtattcc gccgtaggga tcttatggcg ggccaggaaa tctttggtaa acgcttttga
    27781 gccttccagt tgggccgcac ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa
    27841 ggtatcgacc acgcctttca ccagcggcgc ttccgggccg acgatggtca gatcaatctt
    27901 ttcgttttgt gcgaaatcca acagcgccgg gatatcggtc acgccgatag caacgttttg
    27961 cagcgcgggt tccagcgcag tgcctgcatt acccggagca acaaaaacag tctcaaccag
    28021 cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc ccgccgttac caatcactaa
    28081 tactttcatc tattgctcca ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca
    28141 ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac ggatggaacc gccaggctgg
    28201 attacgcagg tcacgcccgc agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac
    28261 gcgtcagaag ccatcgagga acctttcact tccaggcctt catcggcagc tttaataccg
    28321 gcgattttcg cggagtacac gcggctcatc tggcccgcgc caataccgat ggtcatattg
    28381 tttttggcat agacgatagc attggatttc acgaatttcg ccactttcca gcagaacagc
    28441 gcatcacgca gttcctgttc ggtcggctga cgttgggtga cgacgcgcag ttcttccgca
    28501 ccgaccatcc ccaggtcacg atcctgaacc agcagaccgc cgttcacgcg tttgaaatca
    28561 agacccggaa cacgctcgcc ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg
    28621 gcggtgattt tcagggcttc ttcgctggcg gaaggcgcaa taatcacttc gacaaactga
    28681 cgagaaatga tggcctgtgc ggtttccgca tccagctcgc ggttaaaggc aatgatgccg
    28741 ccgaatgcgg aggttgggtc ggttttgtac gcgcgatcgt aagcatcaag aatggaattg
    28801 ccgatagcta caccgcaagg gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac
    28861 tctttcacgc actccagcgc cgcatcggta tcggcgatgt tgttataaga gagggcttta
    28921 ccctgaacct gggttgcggt agcaacggag gcttctttca cattctcttc tatatagaag
    28981 gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg
    29041 ttcagcgtgc gtgggaagcg accggcggct tctttgcttt caccgtggta cgccggaacc
    29101 atgctgccga agtagttggc aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg
    29161 atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt cgttgtcatc catctcttta
    29221 ataatggcgt cgtagtcgct gctcttcacc acgattgcga catctttatg gttcttagcg
    29281 gcggagcgca ccatggtcgg gccgccgata tcgatgttct caaccgcatc ttccagcgag
    29341 cagccttcac gagcaacagt ctgggcgaac ggatacaggt taacaaccac catatcgata
    29401 ggctggatct gatgttcttc cataatggca tcgtcctggc cgcgacggcc cagaatgcca
    29461 ccatgtactt tcggatgcag ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg
    29521 taatcggaaa cttcggttac cggcagacct ttttctgcta acagacgggc agtgccccct
    29581 gtagacagca gctccacacc gcgtgcggaa agtgcctggg cgaattcgac gataccggct
    29641 ttgtcagaaa cactgagcag agcgcggcgg actggacgac gttgttgcat ggtaaatccc
    29701 ctgggtttga ctattacaga gagcgttagc tgaatttttc gcgaaaaact cagctaacgc
    29761 ccctaacggg gcatccttat ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc
    29821 gcgaattttt ctctttcaat ggtgatcaca attttgacag tggttaccgt gggaaaaata
    29881 cagaaattac attgatgatt gtggataact ctgtgcgtaa aaaggtataa agcggggttt
    29941 tgctggggaa tgcagcagtc agtcattttt ctgcaatttt tctattgcgg cctgcggaga
    30001 actccctata atgcgccttc atcgacacgg cggatgtgaa tcacttcaca caaacagccg
    30061 gttcggttga aga
//
LOCUS       DAJTRB010000045        29798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTRB010000045 DAJTRB010000000
VERSION     DAJTRB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.045"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            57..851
                     /locus_tag="OO330_002137"
     CDS             57..851
                     /locus_tag="OO330_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5882998.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            873..2282
                     /locus_tag="OO330_002138"
     CDS             873..2282
                     /locus_tag="OO330_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5882999.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVVAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="OO330_002139"
     CDS             2260..3039
                     /locus_tag="OO330_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5883000.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="OO330_002140"
     CDS             3036..3896
                     /locus_tag="OO330_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5883001.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="OO330_002141"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="OO330_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="OO330_002142"
     CDS             complement(4636..4896)
                     /locus_tag="OO330_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5883002.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="OO330_002143"
     CDS             complement(4887..6158)
                     /locus_tag="OO330_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5883003.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="OO330_002144"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="OO330_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5883004.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="OO330_002145"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="OO330_002145"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5883005.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTQAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="OO330_002146"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="OO330_002146"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5883006.1"
                     /translation="MSEKHPEPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="OO330_002147"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="OO330_002147"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5883007.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="OO330_002148"
     CDS             11500..11652
                     /locus_tag="OO330_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5883008.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11846..12937
                     /locus_tag="OO330_002149"
                     /pseudo
     CDS             11846..12937
                     /locus_tag="OO330_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13083..14120)
                     /gene="iap"
                     /locus_tag="OO330_002150"
     CDS             complement(13083..14120)
                     /gene="iap"
                     /locus_tag="OO330_002150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5883009.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14372..15280
                     /gene="cysD"
                     /locus_tag="OO330_002151"
     CDS             14372..15280
                     /gene="cysD"
                     /locus_tag="OO330_002151"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5883010.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15282..16709
                     /gene="cysN"
                     /locus_tag="OO330_002152"
     CDS             15282..16709
                     /gene="cysN"
                     /locus_tag="OO330_002152"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5883011.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16709..17314
                     /gene="cysC"
                     /locus_tag="OO330_002153"
     CDS             16709..17314
                     /gene="cysC"
                     /locus_tag="OO330_002153"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5883012.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17364..17687
                     /locus_tag="OO330_002154"
     CDS             17364..17687
                     /locus_tag="OO330_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5883013.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17881..18192
                     /gene="ftsB"
                     /locus_tag="OO330_002155"
     CDS             17881..18192
                     /gene="ftsB"
                     /locus_tag="OO330_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5883014.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18211..18921
                     /gene="ispD"
                     /locus_tag="OO330_002156"
     CDS             18211..18921
                     /gene="ispD"
                     /locus_tag="OO330_002156"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5883015.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18921..19400
                     /gene="ispF"
                     /locus_tag="OO330_002157"
     CDS             18921..19400
                     /gene="ispF"
                     /locus_tag="OO330_002157"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5883016.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19397..20446
                     /gene="truD"
                     /locus_tag="OO330_002158"
     CDS             19397..20446
                     /gene="truD"
                     /locus_tag="OO330_002158"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5883017.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20427..21188
                     /gene="surE"
                     /locus_tag="OO330_002159"
     CDS             20427..21188
                     /gene="surE"
                     /locus_tag="OO330_002159"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5883018.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21182..21808
                     /gene="pcm"
                     /locus_tag="OO330_002160"
     CDS             21182..21808
                     /gene="pcm"
                     /locus_tag="OO330_002160"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5883019.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21948..23087
                     /gene="nlpD"
                     /locus_tag="OO330_002161"
     CDS             21948..23087
                     /gene="nlpD"
                     /locus_tag="OO330_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5883020.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23150..24142
                     /gene="rpoS"
                     /locus_tag="OO330_002162"
     CDS             23150..24142
                     /gene="rpoS"
                     /locus_tag="OO330_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5883021.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24262..24669)
                     /locus_tag="OO330_002163"
     CDS             complement(24262..24669)
                     /locus_tag="OO330_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5883022.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24816..25409
                     /locus_tag="OO330_002164"
     CDS             24816..25409
                     /locus_tag="OO330_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5883023.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25409..26356
                     /locus_tag="OO330_002165"
                     /pseudo
     CDS             25409..26356
                     /locus_tag="OO330_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26629..27192)
                     /locus_tag="OO330_002166"
     CDS             complement(26629..27192)
                     /locus_tag="OO330_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883024.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27357..27608
                     /locus_tag="OO330_002167"
     CDS             27357..27608
                     /locus_tag="OO330_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5883025.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27610..29706
                     /locus_tag="OO330_002168"
     CDS             27610..29706
                     /locus_tag="OO330_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5883026.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7301 a         7383 c         8058 g         7056 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggcgtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggtggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggctg ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc agccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacactcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggggaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc caagcggcgg atgaattttt aagtgagctg cgtgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagagcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cttggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaattt attcataatg taaatgtttt attggctgat
    11821 atatatcata gatagttttt aaaatatgcg taaatatcct ttaagtttac tgaaggataa
    11881 aaatattgtg actttctttg atttctgggg aaaaaaccga cgtggcgaga aagagggtat
    11941 cgacgactat caccttcttt gctggcattt gctggatgtg gccgcaatgg gctatttaat
    12001 ggttaaaaga aattgcttcg ggctggctga ttattttcgt caattaggga tttctgacaa
    12061 ggaacaggca gctcaatttt tcgcctggtt gctgtgctgg cacgatattg gaaaatttgc
    12121 ccgctctttt caacaacttt acctgccccc tgaattcaag attcaggaag gtgccagaaa
    12181 aaattacgaa aaaatctctc attcaacgct gggctactgg ttgtggaatc attatttaag
    12241 tgaatgtcag gagttacttc cttcatcttc gctctctccg cgcaaactta ggcgagtgat
    12301 agaaatgtgg atgcccgtga cgacagggca tcatggtcgg ccccctgatc gtatggatga
    12361 attggataat tttctgcctg aagacaaagc tgcagcacga gattttctcc ttgaaatcaa
    12421 gccactgttt ccgcgaatag agatccccac attctgggat gatgacgagg gcgttgaact
    12481 tataaaacaa ctttcctggt atatctctgc aaccgtcgta cttgcagact ggacagggtc
    12541 gtcaacgcga ttttttccac gcgtcgcaca ggcaatggat attgaagatt actggcaaaa
    12601 ggctttagtt caggctcaaa acgcattaac cgtctttccg ccacaagcag aaaccgcgcc
    12661 tttcaccgga attaatacgc tgtttccttt tattgagaat acgacaccgt tacagcaaaa
    12721 ggtgctggat ctggatatca atcagccagg accacagttg tttattctgg aagatgtgac
    12781 cggcgcaggt aaaacagaag cggcgcttat cctggcgcac aggttgatgg ctgcgggtaa
    12841 cgctcagggg ctgttttttg gcctgccaac aatggcaacg gccaatgcca tgtacgatcg
    12901 actggtcaaa acctggcttg ctttctattc gccagaggtg ttccccgctc cagcggagat
    12961 aaaccggcgc aatacggtgc ggttttcccg gaagtaaacc ggacgcactg gatgcgatga
    13021 tgggtatcac ttagaattcc ccgcccctgc ggtagaactc ccagctccca ttttcaaacc
    13081 catcaagacg ccttcgccag ctccttcacc aacggtagca ttatccgcat aacgtcacgg
    13141 cagcgacgtt ctattcttcc aggaagtgcc ttatcaatat gttgttgatt atccagtctt
    13201 acgtcatgcc agctatttcc cgccgggaag gcaggtgttt ttgcgcgttg ctgataacca
    13261 tccttatttc caagattcca gttagtcgct tccaccgaaa gtacagcaat gcccgctttg
    13321 tcgaatattt ctgcgtcatt acaacaccca gtgcctttcg gataattttt attcaaaccc
    13381 ggattggtcg ttgcggctat tccgtgactg cgtgcaattg ccagcgccct gtcgcgcgtt
    13441 aatttcctta ctgcttcagg ggtttttaca ccgctgttga aatacaattt atcgccaaca
    13501 attaagttat cgagattaat caccagcagc gtatttttct tttcggtgtc actcatccgc
    13561 ttgagtaaat tctcagcgcc taattttcct tcctcttcgc cgctggtcgc cacaaatcga
    13621 ataccatact cggtaggcgt atttttcagg cgttctgcca gttccagcat gacacctaaa
    13681 cctgcggcgt tatcatctat tccttgtaac gtcagtccgc cgagattggc atctgcgtca
    13741 gcatcgctca gcggggcgta agtatccaga tgcgccataa tgatgatctg ctgcggcgct
    13801 ttgccttcat gagcggcaat caccgtactt cctgtcacgt tatgccagtt cttgcgatta
    13861 tcgcgggcgg tataaatata ccgactatta aatgtccgaa tatcactgcg ataacccatt
    13921 tgctgaaact gttggcgaat ataatcggca gataacattt ctgctggagt tccggtcatg
    13981 cgtcccggaa agaaagtagc aatatgtcgt gcctgggtat tagcaaaatc gccaggttta
    14041 ggtgacgagg cgtgtacggg gagaataaag catacgccga gcgccagggc agcggtacgg
    14101 tggcgcaatg cggaaaacat agtgagtcct taaataccat gcaaattttt ttaccgccat
    14161 agtatgaaac tgccgctgcg ctaaaacaat ttcaaatctt cctaaacgcc cgaaatccgg
    14221 tgccttaagc actttttgat attagctttg ccaaatcgtt attccgttaa ggaactactc
    14281 attctaattg gtaatttcat tcgttctctt acgctcccta tagtcgaaac atctgatggc
    14341 aagaaaatag cggtattgca aaggaacggt tatggatcaa aaacgactta ctcacctgcg
    14401 gcaactggag gcggaaagca tccacattat tcgcgaggtg gcggcagaat tctcaaatcc
    14461 ggtgatgctc tactctatcg gtaaagattc cagcgtcatg ctgcatctgg cgcgcaaggc
    14521 gttttatcca ggtacgctgc ctttcccgtt gctgcatgtc gataccggct ggaaattccg
    14581 cgagatgtat gagttccgcg atcgtacggc gaaagcctac ggctgcgagc tgctggtgca
    14641 taaaaacccg gaaggcgtgg cgatggggat taatccattc gtgcacggca gcgcgaaaca
    14701 taccgatatt atgaaaactg aaggcctgaa acaggcgctg aacaaatacg gttttgatgc
    14761 cgccttcggt ggtgcgcgcc gtgacgaaga gaaatcccgc gctaaagagc gaatctactc
    14821 tttccgtgac cgcttccatc gctgggatcc gaaaaatcag cgcccggagc tgtggcacaa
    14881 ctacaacggg caaattaaca aaggcgaaag catccgcgtc ttcccgctct ctaactggac
    14941 cgagcaggat atctggcaat acatctggct ggaaaatatc gacattgtgc cgctgtatct
    15001 cgctgcggaa cgtccggttc tggaacgcga cggtatgttg atgatgattg atgacaaccg
    15061 tatcgacctg caaccgggcg aagtgatcaa aaaacggatg gtgcgtttcc gtacgctggg
    15121 ctgctggccg ctgaccggtg cggtggagtc aaatgcacaa acactgccgg aaatcatcga
    15181 agagatgctg gtttccacca ccagtgaacg tcagggtcgc gtgattgacc gcgaccaggc
    15241 gggatccatg gagctgaaaa aacgtcaggg gtatttttaa gatgaacacc gtacttgcac
    15301 aacaaatcgc caatgaaggc ggcgtcgaag cctggatgat tgcgcaacaa cataaaagcc
    15361 tgctgcgttt tctgacctgt ggtagcgtcg atgacggcaa aagtaccctg attggtcgtc
    15421 tgctgcacga tacccgccaa atctacgaag atcagctctc atcgctgcat aacgacagta
    15481 agcgtcacgg cacccagggc gaaaagctgg atctggctct gctggtggac ggcctgcaag
    15541 ctgagcgcga acagggcatc actattgatg tggcctaccg ctatttctct accgagaagc
    15601 gtaaatttat tatcgccgac accccagggc acgagcagta cactcgcaat atggcgactg
    15661 gcgcatcgac atgtgaactg gcgatcttac tgattgatgc ccgtaaaggc gtgctcgatc
    15721 aaacccgtcg tcacagtttt atctccacac tgttggggat caaacatctg gtcgtggcga
    15781 tcaacaaaat ggatctggtg gattacagtg aagagacgtt cacccgtatt cgtgaagatt
    15841 atctgacctt tgccgggcag ctgccggata atctggatat ccgctttgtg ccgctctccg
    15901 cactggaagg tgacaacgtg gcttcgcaaa gtgaaagtat gccgtggtac agcggtctga
    15961 cactgctcga agtgctggaa accgtggaga tccagcgagt ggtggatgcc cagccaatgc
    16021 gcttcccggt gcagtacgtt aaccgcccaa atctcgattt tcgtggctac gccggaatgc
    16081 tggcatccgg tcgcgtggaa gtcgggcaac gtgtaaaagt gctgccctct ggtgtggaat
    16141 caaacgtcgc gcggatcgtg acttttgatg gtgatcgcga agaagtcttt gccggagaag
    16201 cgatcaccct ggtgctgacg gatgagatcg atatcagccg tggtgatctg ctgctggcgg
    16261 cagacgaagc gttaccggcg gtgcagagtg cgtcggtgga tgtggtatgg atggcggaac
    16321 agccgctttc cccgggccag agttatgaca tcaaaattgc cggtaagaag acgcgtgctc
    16381 gtgttgatgg cattcgttat caggttgata ttaataacct tacccaacgc gaagttgaaa
    16441 acctgccgct gaacggcatc ggcctggtgg atctcacttt tgacgagcca ctggtgttag
    16501 atcgctatca gcaaaatccg gttaccggtg ggctgatttt tatcgatcgc ctgagcaatg
    16561 tgaccgtggg tgccggtatg gtgcacgagc cagttagcca ggcaacagct gcgtcatctg
    16621 aattcagtgc attcgaactg gaattgaatg cactggttcg tcgccacttt ccgcactggg
    16681 gcgcgcgcga tttgctgggg gataaataat ggcgctgcat gacgaaaacg tcgtctggca
    16741 tagtcatccg gtcactgtgc aacaacgcga actacaccac ggtcatcgtg gtgtagtgct
    16801 gtggtttacc ggcctctccg ggtccggtaa atcaacggtc gccggggcgc tggaggaggc
    16861 gttacataaa ctcagcgtca gtacgtatct gctggatggc gacaatgttc gccacggatt
    16921 atgcagcgat ctcggtttta gcgatgccga tcgtaaagag aatatccgtc gcgtcggtga
    16981 agtggcgaat ttgatggttg aagccggact ggtggtgctg accgcattta tctcgccaca
    17041 ccgcgccgaa cgccagatgg ttcgcgaacg cgtaggagaa gggcgcttta ttgaagtgtt
    17101 tgtcgatacg ccgctggcga tttgcgaagc ccgcgatcca aaaggcttat ataagaaagc
    17161 gcgtgccggt gaactgcgca actttacggg aatagattcc gtttacgaag cgcctgaatc
    17221 ggcagaaatt catctcaatg gtgaacaatt agtaacaaat ttggtacagc aattattaga
    17281 tctgttgaga cagaacgata ttatcagatc ctgagacacc accgggtttc catgcccggt
    17341 tcgtcaaagt aacaggatca aatatgcgta atagccataa cattacacta acaaataacg
    17401 acagccttac tgaggatgaa gaaactacat ggtcactgcc tggtgccgtg gtcggtttta
    17461 tctcctggtt atttgcgctg gcgatgccga tgttgattta tggctctaac acgctgttct
    17521 tctttctcta cacctggcct ttctttctgg cactgatgcc tgtcgcggta gtggtgggga
    17581 ttgcgctgca ttcattgatg gacggaaagc tacgctacag tattgttttc accctggtga
    17641 cagtaggcat tatgtttggc gcactgttta tgtggctact gggctaattt gtactttctt
    17701 gtctcctctg ttcataattc aaaccgtaac taataatgag attatgttct gcacgccctg
    17761 ggtatacgta acaatggaca aatgtggtac atttgcccgc gttgtcgcgg tatccccaac
    17821 agaggatgta gagtcgtctt cggatgcatg ggatgatgat gcagtttttc agggggcagg
    17881 atgggtaaac taacgctgct gttgctggct attctggtct ggctacagta ttcgctgtgg
    17941 ttcggtaaga acggtataca tgactatacc cgcgtcaatg atgatgtggc ggcactgcaa
    18001 gctacaaacg cgaaacttaa agcgcgaaac gatcaacttt ttgccgaaat tgacgatctc
    18061 aatggcggcc aggaggcgct cgaagagcgt gcgcgtaatg aactcagcat gaccaggccg
    18121 ggcgaaactt tttatcgtct ggtgcctgac gcgtcgaagc gcgcacagtc tgcggggcaa
    18181 aacaatcgat aaatcagccc gggaattaac atggcaacca ctcatttgga tgtttgcgcc
    18241 gtggttccgg cggccggatt tggccgtcga atgcaaacgg aatgtcctaa gcaatatctc
    18301 tcaatcggta atcaaaccat tcttgaacac tcggtgcatg cgctgctggc gcatccccgg
    18361 gtgacacgtg tcgtcattgc cataagtcct ggcgatagcc gttttgcaca acttcctctg
    18421 gcgaatcatc cgcaaatcac cgttgtagat ggcggtgatg agcgtgccga ttccgtgctg
    18481 gcaggtctga aagccgctgg cgatgtgcag tgggtattgg tgcatgacgc cgctcgtcct
    18541 tgtttgcatc aggatgacct cgcgcgattg ttggcgttga gcgaaaccag ccgcacggga
    18601 gggatcctcg ccgcaccagt gcgcgatact atgaaacgtg ccgaaccggg caaaaatgcc
    18661 attgctcata ccgttgatcg caacggctta tggcacgcgc tgacgccgca atttttccct
    18721 cgtgagctgt tacatgactg tctgacgcgc gctctaaatg aaggcgctac cattaccgac
    18781 gaagcctcgg cgctggaata ttgcggattt catccgcagt tggtcgaagg ccgcgcggat
    18841 aacattaaag tcacgcgccc ggaagatttg gcactggccg agttttacct cacccgaacc
    18901 atccatcagg agaatacata atgcgaattg gacacggttt tgacgtacac gcctttggcg
    18961 gtgaaggccc aattatcatt ggtggcgtac gcattcctta cgaaaaagga ttgctggcgc
    19021 attctgatgg cgacgtggcg cttcatgcgt tgaccgatgc attgcttggc gcggcggcgc
    19081 tgggggatat cggcaaactg ttcccggata ccgatccggc atttaaaggt gccgacagcc
    19141 gcgagctgct acgcgaagcc tggcgtcgta ttcaggcgaa gggttatacc cttggcaacg
    19201 tcgatgtcac tatcatcgct caggcaccga agatgttgcc gcacattcca caaatgcgcg
    19261 tgtttattgc cgaagatctc ggctgccata tggatgatgt taacgtgaaa gccactacta
    19321 cggaaaaact tggatttacc ggacgtgggg aagggattgc ctgtgaagcg gtggcgctac
    19381 tcattaaggc aacaaaatga ttgagtttga taatctcact tacctccacg gtaaaccgca
    19441 aggcaccggg ctgctgaaag ccaatccgga agactttgtg gtggtggaag atttgggctt
    19501 tgagcctgat ggtgaaggtg agcatattct ggttagaatc ctcaaaaacg gctgcaatac
    19561 ccgttttgtg gcggatgcac tggcgaaatt cctgaaaatt cattcccgtg aagtcagctt
    19621 cgctgggcaa aaagacaaac atgctgtaac ggaacagtgg ttatgtgctc gcgtgccggg
    19681 caaggaaatg cccgatctga gcgcctttca actggaaggc tgccaggtgc tggagtatgc
    19741 gcggcacaag cgcaagctgc gtttaggggc gctgaaaggt aacgccttta ccctggttct
    19801 gcgcgaagtg agcaatcgcg atgacgttga acaacgtctg atcgatattt gcgtaaaagg
    19861 tgtaccgaac tacttcggtg cccaacgttt tgggattggc ggtagcaact tgcagggcgc
    19921 gctgcgctgg gcgcaaacca atactccggt gcgcgatcgc aataaacgga gtttttggtt
    19981 gtcggcagcc cgcagtgcgt tgtttaatca gattgttgct gagcgcctca aaaaagcaga
    20041 cgttaatcaa gttgttgacg gcgatgcgct acaattagcc ggacgtggta gctggtttgt
    20101 agcaaccacc gaagaactgg cggaattaca gcgtcgcgtc aacgataaag agctgatgat
    20161 aaccgccgca ttgccgggca gtggcgaatg gggaactcag cgtgaagcgc tggcattcga
    20221 acaagcagct gtcgccgcag aaactgaatt acaagcttta ctggtgcgcg aaaaagttga
    20281 agccgcgcgc agagcgatgc tgctgtatcc gcaacaatta agctggaact ggtgggatga
    20341 cgtcaccgta gagatccgtt tctggcttcc ggcgggtagt tttgcaacca gcgttgtcag
    20401 ggaacttatc aacacaacag gtgattatgc gcatattgct gagtaatgat gacggggtac
    20461 atgcacccgg tatacaaacg ctggcgaaag ccttgcgtga gtttgctgac gttcaggtgg
    20521 tcgcccccga tcgtaaccgc agcggcgctt caaattctct gacactggaa tcctccctgc
    20581 gcacgtttac ctttgaaaat ggtgatattg ctgtgcaaat gggaaccccg accgattgtg
    20641 tctatcttgg cgtgaatgct ctgatgcgtc cgcgcccgga cattgttgtg tccggaatta
    20701 acgccgggcc gaatctgggg gatgatgtta tttattccgg tacggtagcc gccgcgatgg
    20761 aaggccgtca tttaggtttt ccggcgctgg ccgtctcgct tgacgggcat aaacattacg
    20821 acactgccgc ggcggtaacc tgttcaattt tgcgcgcact gtgtaaagag ccgctgcgca
    20881 ccggacgtat tcttaatatt aacgttcagg atttaccctt ggatcaaatc aaaggtattc
    20941 gcgtgacgcg ctgcggtaca cgacatccgg cagatcaggt gatcccgcag caagatccgc
    21001 gcggcaatac gctgtactgg attggcccgc cgggcggtaa atgtgatgcc ggtccgggga
    21061 ccgattttgc tgcggtagat gagggctatg tctccatcac gccgctgcat gtggatttaa
    21121 ctgcgcatag cgcgcaagat gtggtttcag actggttaaa cagcgtggga gttggcacgc
    21181 aatggtaagc agacgcgtac aagcacttct ggatcaatta cgtgcgcaag gtattcagga
    21241 tgagcaggtg ctgaatgcac ttgccgccgt gccgcgtgaa aaattcgttg atgaagcgtt
    21301 tgaacaaaaa gcctgggaca atatcgcctt gccgataggt caggggcaga caatttcgca
    21361 gccgtatatg gtggcgcgaa tgaccgaatt actcgagctg acgccgcagt cgcgggtgct
    21421 ggaaattggc actggttcgg gatatcaaac ggcaatcctg gcgcatcttg tccagcatgt
    21481 ttgctcggtt gaacggatta aaggcttgca gtggcaggca cgtcgccgcc tgaaaaatct
    21541 tgatttacat aatgtttcaa cccgtcatgg tgatggatgg caaggttggc aggcacgtgc
    21601 gccgtttgac gctatcattg ttacggcggc accgccggaa attccaactg cgctaatgac
    21661 gcagctggac gaaggcggga ttctcgtctt acccgtaggg gaggagcacc agtatttgaa
    21721 acgggtgcgt cgtcggggag gcgaatttat tatcgatacc gtggaggccg tgcgctttgt
    21781 ccctttagtg aagggtgagc tggcttaaaa cgtgaggaaa tacctggatt tttcctggtt
    21841 attttgccgt aggtcagcgt atcgtgaaca tcttttccag tgttcagtag ggtgccttgc
    21901 acggtaatta tgtcactggt tattaaccaa tttttcctgg gggataaatg agcgcgggaa
    21961 gcccaaaatt caccgttcgc cgcattgcgg ctttgtcact ggtttcgcta tggctggcag
    22021 gctgttctga cacttcaaat ccaccggcac cggtcagctc cgttaatggc aatgcgcctg
    22081 caaatactaa ttctggtatg ttgattacgc cgccgccgaa aatggggacg acgtctacag
    22141 cgcagcaacc gcaaattcag ccggtacagc agccacaaat tcaggctact caacaaccgc
    22201 aaatccagcc agtgcagcca gtagctcagc agccggtaca gatggaaaac ggacgcatcg
    22261 tctataaccg tcagtatggg aacattccga aaggcagtta tagcggcagt acctataccg
    22321 tgaaaaaagg cgacacactt ttctatatcg cctggattac tggcaacgat ttccgtgacc
    22381 ttgctcagcg caacaatatt caggcaccat acgcgctgaa cgttggtcag accttgcagg
    22441 tgggtaatgc ttccggtacg ccaatcactg gcggaaatgc cattacccag gccgacgcag
    22501 cagagcaagg agttgtgatc aagcctgcac aaaattccac cgttgctgtt gcgtcgcaac
    22561 cgacaattac gtattctgag tcttcgggtg aacagagtgc taacaaaatg ttgccgaaca
    22621 acaagccaac tgcgaccacg gtcacagcgc ctgtaacggt accaacagca agcacaaccg
    22681 agccgactgt cagcagtaca tcaaccagta cgcctatctc cacctggcgc tggccgactg
    22741 agggcaaagt gatcgaaacc tttggcgctt ctgagggggg caacaagggg attgatatcg
    22801 caggcagcaa aggacaggca attatcgcga ccgcagatgg ccgcgttgtt tatgctggta
    22861 acgcgctgcg cggctacggt aatctgatta tcatcaaaca taatgatgat tacctgagtg
    22921 cctacgccca taacgacaca atgctggtcc gggaacaaca agaagtgaag gcggggcaaa
    22981 aaatagcaac catgggtagc accggaacca gttcaacacg cttgcatttt gaaattcgtt
    23041 acaaggggaa atccgtaaac ccgctgcgtt atttgccgca gcgataaatc ggcggaacca
    23101 ggcttttgct tgaatgttcc gtcaagggat cacgggtagg agccacctta tgagtcagaa
    23161 tacgctgaaa gttcatgatt taaatgaaga tgcggaattt gatgagaacg gagttgaggt
    23221 ttttgacgaa aaggccttag tagaagagga acccagtgat aacgatttgg ccgaagagga
    23281 actgttatcg cagggagcca cacagcgtgt gttggacgcg actcagcttt accttggtga
    23341 gattggttat tcaccactgt taacggccga agaagaagtt tattttgcgc gtcgcgcact
    23401 gcgtggagat gtcgcctctc gccgccggat gatcgagagt aacttgcgtc tggtggtaaa
    23461 aattgcccgc cgttatggca atcgtggtct ggcgttgctg gaccttatcg aagagggcaa
    23521 cttggggctg atccgcgcgg tagagaagtt tgacccggaa cgtggtttcc gcttctcaac
    23581 atacgcaacc tggtggattc gccagacgat tgaacgggcg attatgaacc aaacccgtac
    23641 tattcgtttg ccgattcaca tcgtaaagga gctgaacgtt tacctgcgaa ccgcacgtga
    23701 gttgtcccat aagctggacc atgaaccaag tgcggaagag atcgcagagc aactggataa
    23761 gccagttgat gacgtcagcc gtatgcttcg tcttaacgag cgcattacct cggtagacac
    23821 cccgctgggt ggtgattccg aaaaagcgtt gctggacatc ctggccgatg aaaaagagaa
    23881 cggtccggaa gataccacgc aagatgacga tatgaagcag agcatcgtca aatggctgtt
    23941 cgagctgaac gccaaacagc gtgaagtact ggcacgtcga ttcggtttgc tggggtatga
    24001 agcggcaaca ctggaagatg taggtcgtga aattggcctc acccgtgaac gtgttcgcca
    24061 gattcaggtt gaaggcctgc gccgtttgcg cgaaatcctg caaacgcagg ggctgaatat
    24121 cgaagcgctg ttccgcaagt aagtaagcat ctgtcagaaa ggccagtctc aagcgaggct
    24181 ggcctttttc ttttgtttgg gaagcttgtg tttttgtcgc ctggataaga cacgtcagcg
    24241 tcctacccgg caatcagcat tctatgtgct catcatcttg cgcaccaact gcataaacag
    24301 ctcctgctct tctccactca aacgccccaa aaattcctca tccacgctgt caccaatagg
    24361 tatcgccgca gcaagtagct tttctccttc ggcagtcagc cagacaaagc gtcgccgctt
    24421 gtcagcgacg tcatgttcac gtctgactaa gccacgattt tccattcttg ccaacatttc
    24481 tgctagcgtt gctttggtgc tgactgctgc ttctatcagt gccacctgtt caataccagg
    24541 tttatcagca atggcgcgca tcaccgcgta ctgtggtttg gtcaggtcag gtaactcgtg
    24601 ctgccagcga gccgtgtgct gctgaaaaag ctgtcgtaac tgatggaacg ctttatttcg
    24661 taacgccatg taaactccgg gctaattctc tgtcgctatc atagcggttt tttagtgacg
    24721 aagaagatgt tgttactttt caatccttgc cgctgtggaa atgaaggagt atgttaataa
    24781 taacgttcgc atacgaataa ttaagagaat aagcaatgaa actgatcgtc gggatgacag
    24841 gggctaccgg tgcgcctctt ggtgtggcat tactgcaagc gctgcgtgag atgccgaatg
    24901 tcgagactca tctggtgatg tcgaagtggg cgaaaaccac cattgaactg gaaacgcctt
    24961 acagcgctcg cgatgttgct gccctcgcag acttcagcca taacccggcg gatcaggcgg
    25021 cgatcatctc atccggttct tttcgtaccg acggcatgat cgttattccg tgcagtatga
    25081 aaacgctcgc cggtatccgc gctggttacg ccgatggcct ggtagggcgc gcggcggacg
    25141 tcgtgctcaa agaaggccgc aaactggtgc tggtgccgcg tgaaatgccg cttagcacca
    25201 tccatctcga aaatatgctc gcactttcac gcatgggcgt ggcgatggtg ccgccgatgc
    25261 ctgcctttta taaccatccc gaaacggtag atgacattgt ccaccatgtg gtagcccgcg
    25321 tgctggatca atttggcctc gaacatcccc acgccaggcg ctggcaagga ttaccgcagg
    25381 cccggaattt ttctcaggag aatgaataat ggcatttgat gatttacgca gctttttaca
    25441 ggcgcttgat gaccacggcc agttactgaa aatcagcgaa gaagtgaacg ctgagccgga
    25501 tctggcagca gcagctaacg ccaccgggcg tatcggcgac ggcgcgcccg cgctgtggtt
    25561 tgataatatt cgcggcttta ccgatgcccg cgtggcgatg aataccatcg gttcctggca
    25621 gaaccacgcg atttcccttg gcctgccacc aaacaccccg gtcaaaaagc agattgatga
    25681 gtttatccgc cgctgggata acttcccgat tgccccggag cgccgcgcca atccagcctg
    25741 ggcgcagaac accgtcgatg gcgaagagat caacctgttc gatattctgc cgctgtttcg
    25801 cctgaatgat ggcgatggcg gtttctatct cgacaaagcg tgcgtggttt cccgcgatcc
    25861 gctcgacccg gataacttcg gcaagcagaa cgtcggcatc taccgcatgg aagtgaaggg
    25921 caagcgtaag ctcggcctgc aaccggtgcc gatgcacgat atcgccctgc atctgcataa
    25981 agcagaagag cgcggtgaag atctgccgat tgcgatcacg ctcggtaacg atccgatcat
    26041 cacgctgatg ggggccacgc cgctgaaata cgatcagtct gagtatgaaa tggcaggcgc
    26101 gctgcgtgaa agcccgtacc cgatcgccac cgctccgctg accggttttg atgtgccgtg
    26161 gggttcagaa gtgatcctcg aaggggttat cgaaagccgt aaacgcgaaa tcgaagggcc
    26221 gttcggtgag tttaccgggc actactccgg tgggcgcaac atgaccgtgg tgcgcatcga
    26281 taaagtctct tatcgcagca aaccgatttt cgaatcactc tatctcggta tgccgtggac
    26341 cgaaatcgac tacctatgac taggtaattt attcaagata accgggaaga gattaaaaaa
    26401 gacgggaata gattggaatc aacaagttag gaactcaagg ttttcaagat aaagcgagtc
    26461 caaaaatgtt aaacggttcg atatcaataa aacattcaag ataactcaca ggttcttaca
    26521 atagccttta aagaccgttc aaaaacgatt caggtgttta ttatagcgct gatgggtgca
    26581 agcgccaaaa ccaatgtaat tttagcacag ctaggcactt gcactgggtt atgcgctggc
    26641 tgctttctga ggtagattta agtgatcgcc cttagcttcg gacaacgcgt aaatctctct
    26701 aaccttttcg tctggcaaat taccaaggct taacgctatg gctctacgaa ctccaactag
    26761 agcgtgcaac tcttggatgt gctcatccag aagaatgagt aattgctccg ctccttttct
    26821 cccaagaaca tcagctactt gttgtttttc ttgtggttta agaaatgaca agaacattag
    26881 aatttgagca tcaacattat ccgcattctc ttttttgggt tctttggaat cttttagttc
    26941 aacatcaccc tctcccgtca tgagccattc caaggtaaca ttttcgcttt ctgctatctt
    27001 ttttaactga tgcgggcgtg gtatcggtgc gtaatcccgc ctgttgtaat aattttttaa
    27061 agtgccttca ttgatccccc atgcccgagc agccgcattt cttgaagggt atcgttctac
    27121 taacctaaag attctttcga taatcggttc ttttccatcg gcaggaaaag aaccttgggg
    27181 tgcttttctc attcttggtt cctttctatc taattcattg ataaataata aaattaaaaa
    27241 ataaaccata aaaacaccaa agaacccttt acagggaatc tttggtatct taatctttat
    27301 ccatacggat atccgaccgg atatccgcgc gggtaatttt gtaggttaaa ggaaagatga
    27361 gacaaagaga agatagtgac cgcgattggc cttcgctgaa gattcagctg gcgctcaaag
    27421 ataacgggtg gtcttccttc cgggagcttt cactccatgc tggttatgct caggattcac
    27481 tgaaagcggt agttcgcaca ccaaacaaaa aatacgaacg cgtaatttct gacgctatag
    27541 gtgtaccgcc agaaatcatc tggcccagcc ggtatcgtaa cgatggattt atcaggaagg
    27601 cgtcgtgata tgcagtcgta cgttaccgta aatgacctgc tcggcgtacc aggaatgcca
    27661 gccaccacaa aaggcatccg acaggcgtta cagcgttttt ctcgtgatct tggtgacgtt
    27721 agtcgccgtc gggaaggcac aaaagccatt gagtaccaca ttgattgtct gcctgaaata
    27781 acacgaaagg cactgcgtga acgctatgtg gaacagctgg tggctacaga gaacaatgtt
    27841 tctgaagtga aagctgttac cagaaaaaca cgcaatcctg acgctgtcca ggcaatcgaa
    27901 gcatatcgcg gttctccaca actgatggaa gaacgcctga atgcgctgac cgaaaaccag
    27961 cgctgggtat ctgaagcaag agctgcgctg gtggtggaag tgctgaagct ggaaagcgcc
    28021 ggtaaccccg ggcgactgaa agccattaac tttcttgttg aaaaagcccg taaaggtgag
    28081 ctgccggagc gcctgcaaca ggccgcagtt aacgccaatg caaaacgtgg cgctaatcgt
    28141 actatcagcc gcgatccact ttaccagtgg gttctgaaat acaaccagtc gcagaacgcc
    28201 gccgaacggc tgctgttact ggcaccggga aaacgcgacg aaataaaacc ggaagaaatc
    28261 agctggctgc ctgaatttct ggcgcagtat cgccaggtga acggcagacc catgtcagaa
    28321 gcctacgagg attttgtcgc agaatggcag cgtcgccatg cggacgaacc gtacatgctg
    28381 gaggtgatgc cttcctatga tgttgtccgg tatgccatga aaaaactgcc ggaagtggtg
    28441 aaacaaaaag ggcgcgttac cggcagtgaa taccgtcagc ttgaaggatt cacccgccgc
    28501 gactggacag ctatgccggt gaattatgtc tggattggtg acggtcacgg tatgaagctg
    28561 aaatgtgcgc atccgatcca cgggcgtcca ttctcaccgg aagtcacgtt tgtgatcgat
    28621 ggtggaacac ggtttgttgt tggctggagt cttgaccttg ccgaaaacgt gttcgccgtt
    28681 gccggtgcga tacagcacgg cattcgcaac cacggaaaac cgttcctgta ttactcggat
    28741 aacggttccg gtgaaacggc tgacatgctg gacaaagagg tcgtgggtat tctgccacgc
    28801 ctggggatta aacaccctac cggtattgcc ggtaatccac agggacgcgg cattattgaa
    28861 cgcctgaacc ggacactgcc gatgcgtata gccaggaggt atcgcaccta ttttggcaag
    28921 ggtgctgacc gggaatcgtt gcgtgtactt aaccgtgatc tgcgttcagc gtttaacgcc
    28981 ctgcaacagg acaagcctct gaacgaccgc cagaaagcgg caatgcgcga gctgccatcg
    29041 tgggcggagc ttattgaggc tattcgtgaa ggcgtggaat ggtacaacaa ccgcccgcat
    29101 tctgagctgc cgatgaaacc gaacggacgg cattacagcc cgacagagtt cagaaaaaaa
    29161 cgccaggcag aagaagacac cgaaattgag tggctgagtg atcttgagct gcgcgacatg
    29221 ttccgcccaa tggtggaacg accggtaaga cgctgcgaaa ttcagtggct gaacaatatt
    29281 tactacgcgc cggagttgcg tgacgaacac ggtcgcaaag tgcttatcag ttatgacatt
    29341 catgacgccg agcgaattac ggtacgtcgc aaggacggca gctttatctg tgaggcgata
    29401 tggaacggca ataaacgcgc agcctttgct gtcagtgctg aatatcacaa acaacagcag
    29461 cgtattaaag gaatgcgcaa acgcgctgag gaaaaaattc gtgatgctga agatgaaggt
    29521 attcagattc tggaacacaa acaggctgaa ccctggctga gtaatgttta tcgcccggtt
    29581 ggtaatgtgg tggcagttca gcaaccggaa tatgaagaag agcatgacga agaatttgaa
    29641 cgcgatttcc ggctggggat gcaaaaactt tttgccatgc aggaagaaga tgatccgctg
    29701 gcctgaataa aaaaacagtc cgcgggaacg gactgttaaa taaaggaaac aaagctgatt
    29761 tgaaataacc gaggaaatac tatgactgat attgatga
//
LOCUS       DAJTRB010000046        29115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTRB010000046 DAJTRB010000000
VERSION     DAJTRB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.046"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..211
                     /locus_tag="OO330_002169"
     CDS             <1..211
                     /locus_tag="OO330_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5883027.1"
                     /translation="RRWARNGNIYPAPELHGRSYRVVPEAFYINPNKVDTDITHHQPN
                     GRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            177..1253
                     /locus_tag="OO330_002170"
                     /pseudo
     CDS             177..1253
                     /locus_tag="OO330_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1646..1987)
                     /locus_tag="OO330_002171"
     CDS             complement(1646..1987)
                     /locus_tag="OO330_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5883028.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2000..2872)
                     /gene="copD"
                     /locus_tag="OO330_002172"
                     /pseudo
     CDS             complement(2000..2872)
                     /gene="copD"
                     /locus_tag="OO330_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(2876..3250)
                     /gene="yobA"
                     /locus_tag="OO330_002173"
     CDS             complement(2876..3250)
                     /gene="yobA"
                     /locus_tag="OO330_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5883029.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3389..3619
                     /gene="holE"
                     /locus_tag="OO330_002174"
     CDS             3389..3619
                     /gene="holE"
                     /locus_tag="OO330_002174"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5883030.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3721..4377
                     /locus_tag="OO330_002175"
                     /pseudo
     CDS             3721..4377
                     /locus_tag="OO330_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4401..5063
                     /gene="exoX"
                     /locus_tag="OO330_002176"
     CDS             4401..5063
                     /gene="exoX"
                     /locus_tag="OO330_002176"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5883031.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5060..7120)
                     /gene="ptrB"
                     /locus_tag="OO330_002177"
     CDS             complement(5060..7120)
                     /gene="ptrB"
                     /locus_tag="OO330_002177"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5883032.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7330..7989)
                     /locus_tag="OO330_002178"
     CDS             complement(7330..7989)
                     /locus_tag="OO330_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5883033.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8316..8672)
                     /locus_tag="OO330_002179"
     CDS             complement(8316..8672)
                     /locus_tag="OO330_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5883034.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8739..9029)
                     /gene="yebG"
                     /locus_tag="OO330_002180"
                     /pseudo
     CDS             complement(8739..9029)
                     /gene="yebG"
                     /locus_tag="OO330_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9163..10341
                     /gene="purT"
                     /locus_tag="OO330_002181"
     CDS             9163..10341
                     /gene="purT"
                     /locus_tag="OO330_002181"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5883035.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10397..11038)
                     /gene="kdgA"
                     /locus_tag="OO330_002182"
                     /gene_synonym="eda"
     CDS             complement(10397..11038)
                     /gene="kdgA"
                     /locus_tag="OO330_002182"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5883036.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11075..12886)
                     /gene="edd"
                     /locus_tag="OO330_002183"
     CDS             complement(11075..12886)
                     /gene="edd"
                     /locus_tag="OO330_002183"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5883037.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALAFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13121..14596)
                     /gene="zwf"
                     /locus_tag="OO330_002184"
     CDS             complement(13121..14596)
                     /gene="zwf"
                     /locus_tag="OO330_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5883038.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14934..15803
                     /gene="hexR"
                     /locus_tag="OO330_002185"
                     /gene_synonym="yebK"
     CDS             14934..15803
                     /gene="hexR"
                     /locus_tag="OO330_002185"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5883039.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15931..17373
                     /gene="pyk"
                     /locus_tag="OO330_002186"
     CDS             15931..17373
                     /gene="pyk"
                     /locus_tag="OO330_002186"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5883040.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17504..18475)
                     /gene="lpxM"
                     /locus_tag="OO330_002187"
                     /gene_synonym="msbB"
     CDS             complement(17504..18475)
                     /gene="lpxM"
                     /locus_tag="OO330_002187"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5883041.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18595..19917)
                     /gene="mepM"
                     /locus_tag="OO330_002188"
     CDS             complement(18595..19917)
                     /gene="mepM"
                     /locus_tag="OO330_002188"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5883042.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(19933..20865)
                     /gene="znuA"
                     /locus_tag="OO330_002189"
     CDS             complement(19933..20865)
                     /gene="znuA"
                     /locus_tag="OO330_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5883043.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            20944..21699
                     /gene="znuC"
                     /locus_tag="OO330_002190"
     CDS             20944..21699
                     /gene="znuC"
                     /locus_tag="OO330_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5883044.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21696..22481
                     /gene="znuB"
                     /locus_tag="OO330_002191"
     CDS             21696..22481
                     /gene="znuB"
                     /locus_tag="OO330_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5883045.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGMVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22628..23638)
                     /gene="ruvB"
                     /locus_tag="OO330_002192"
     CDS             complement(22628..23638)
                     /gene="ruvB"
                     /locus_tag="OO330_002192"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5883046.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23647..24258)
                     /gene="ruvA"
                     /locus_tag="OO330_002193"
     CDS             complement(23647..24258)
                     /gene="ruvA"
                     /locus_tag="OO330_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5883047.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24397..24462)
                     /gene="yobI"
                     /locus_tag="OO330_002194"
     CDS             complement(24397..24462)
                     /gene="yobI"
                     /locus_tag="OO330_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5883048.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24532..25134
                     /locus_tag="OO330_002195"
     CDS             24532..25134
                     /locus_tag="OO330_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5883049.1"
                     /translation="MNINYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25136..25657)
                     /gene="ruvC"
                     /locus_tag="OO330_002196"
     CDS             complement(25136..25657)
                     /gene="ruvC"
                     /locus_tag="OO330_002196"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5883050.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25692..26432)
                     /locus_tag="OO330_002197"
     CDS             complement(25692..26432)
                     /locus_tag="OO330_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5883051.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26461..26913)
                     /gene="nudB"
                     /locus_tag="OO330_002198"
     CDS             complement(26461..26913)
                     /gene="nudB"
                     /locus_tag="OO330_002198"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5883052.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPVAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27031..28803)
                     /gene="aspS"
                     /locus_tag="OO330_002199"
     CDS             complement(27031..28803)
                     /gene="aspS"
                     /locus_tag="OO330_002199"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5883053.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
BASE COUNT         7133 a         7487 c         7149 g         7346 t
ORIGIN      
        1 gagacgatgg gcaaggaatg gaaacattta tcctgcacct gaactacacg ggaggagtta
       61 cagggtggtt ccggaggctt tctatatcaa cccaaataag gttgataccg atataacaca
      121 ccatcagcct aatgggcgac aagggagaga cagtccgtta atggagaagt taaaacatgc
      181 agcggaaaaa atacgatccc aatttgccta gaaacttaac atatcgaagg agagacaaag
      241 catattactg gcgcaaccct ctgacgaaag aagaatttac actaggtaaa atttcaagaa
      301 gagatgcagt agcgcaggca attgaagcaa atcattatat atacaaaaac tactctcctg
      361 ctgccctaat tgaaaagctt aaaggggtcg actcatttac tatggcaggc tggattgaac
      421 gttacaaaac gattcttata aggagaaaag cgtccagaaa tacttataaa actcgggtaa
      481 atcaactgga gacaataaaa gaaaaattag gaggggtttt actgacagaa ataaccactc
      541 gccatatcgc cgagtttctt gatttgtaga ttgaaggagg gaaaaacacg atggcaggat
      601 caatgcgttc tgtgttatct gatatgttcc gcgaagccat tgttgaagga cgtatatctc
      661 aaaatccagt aacgccaaca agagcaccga aaatagtagt tacaagagaa cggctgaaac
      721 taaagacata caactgcatc agggaggcag cagatcaact tccggcatgg ttcccattag
      781 ctatggactt agcccttgta acaggacaac gtcgcgaaga cataacgaat atgcggttca
      841 gtgatattta tgatgatcgt ctccacatca ggcaaattaa gacaggaatg atgattgcta
      901 tccccctgtc actcagcctt cctgtcgctg gtttacgact tggtgcagta gttgaacagt
      961 gccgcatggt aagtaggggg gattatctaa tcagtgccgg gattagaaaa aacagccctg
     1021 acggcagcat tcacccggac ggcctgacaa agaaatttgt cgcagccaga aaattaacag
     1081 gtatcctgtt cagtgaaaac ccaccaactt ttcacgaaat cagaagcctg gctggacgat
     1141 tgtacaaaga aatatgtgga gaagaatttg ctcagcgtct acttggccac acatcggaga
     1201 agacaacaaa aatgtatctt gatgagagag aaaaaacgta cttactgctc tgattttaac
     1261 gtaaatggat tgttaaatgt attttggttg tgatataacc aaaaaagacc ggaatacaga
     1321 aattcgagta aatttcgggg aatttcgggg agacgtttgc aactaattga ttttaaatac
     1381 aattaaaaaa agaccgaata cgattcctgt attcggtcca gggaaatggc tcttgggaga
     1441 gagccgtgcg ctaaaagttg gcattaatgc aggcttagtt gccttgccct ttaagaatag
     1501 atgacgacgc caggttttcc agtttgcgtg caaaatggtc aataaaaagc gcggtgctca
     1561 tcagcttaaa tgttaaaaac cgcccgttct ggtgaaagaa ctgaggcggt ttttttattg
     1621 gaaatcaaaa ggctatttta ggtaattaac agagtttttc agctcgttct ataaacggtg
     1681 ccagactcat tttttcgccg ggattgttag gatcatcaat ctgaatcacc gaaatgggtt
     1741 gggcattggt cttcccactg gcaacttcct tttgtgcgat atcgtttaaa ggatactgca
     1801 cgagggtact tgggttgatg acatacaaag cattacccgg tcggcaagtc agcattacct
     1861 cttcgcgatt aaacgcccat ttgtctttac ccacttcaaa acgactcacg gttatcacct
     1921 gtggagccgc cagcgccgct gcagaactgg tgagtaacag aaacgccaga atactttttt
     1981 tcatcatttt cgtaatccat caaaaaggtt cgagagtcgc aagcaggctg atgatcacca
     2041 gcactaccgc acctatcgcc cattcgagtt tagtcatcca cacaaaccag ggtgccgcac
     2101 gatcttcatc ctgcctcatg cgcggtacaa gaacataacg attagccagc gcaatgacca
     2161 ccataatcat caccaggatg gctttgagca ataagagttg gccccagtaa gtcgtaagcg
     2221 tggggggaaa tccggtaatc aacaatgcat taagcacgcc gcttgccagt acgccgatca
     2281 ccgcaaaatg cccgcatcag gaaaaacgca tcagcgcctg aatagcctag tgtcgccaac
     2341 ggcctttgat gagttgcata caccagagca ccggcagtaa tcccccaaac cacgccgccg
     2401 cacaaatcag gtgaatcgcg tgattagtct gatggatttt cgctgttacc ccttcattca
     2461 gcgtcgcatg ccccactccc gccagcagaa taaattgcgc ggtagtgagc ataaacagca
     2521 aacgtggcat attccgcggt tgcataaggg cgacgatcaa cgttacgagg gcgagaacaa
     2581 tttgccataa ccagacgcca ccaaactgcg tttgcaaaac agcctgccaa atgtttggcg
     2641 aaaatacatc tgtccatccc gttcccatca acccgccctg aacggcgagc attgcagtgg
     2701 cactgatcaa actccagacg gcagcgtgct gttgtaaacg cagagaacgc ttcgtcagca
     2761 agcgacgaat cattaagggg gccagccagg caccatacat tgcaaagcca aagaccagca
     2821 tcaacgaggt gaaatggata aatcgcagcg cgatccaggt aaacgccagc atgatttatt
     2881 tcacgctaaa ggtgtagtgt cctttcgttt tatgcccgtc caccgaaaca acatgccagt
     2941 caacggtgta ggttccgggt ttcagcgaat cggccagtgg aacaatcagt tgcttttggt
     3001 cctgctcatt ccgcttcgct ggtaatgttt taatattttc gttttttggc cccgtgattt
     3061 ttgcaccact gaatcctgtt tcaacgcctt ccgagaagtt taaagtgatt gcctgcgggg
     3121 cagctgtcac ttgcgcgttt gccgcaggat actgatgcgt taaatgcgca tgtgcccaga
     3181 cggaaggtgt caccaacgaa gtggtcagga ttgccagcgc gtagcgaagg gagcgtgcag
     3241 ttgaagccat attatctatt cctttttgta ataacttttt tacagagcat aaccttgtct
     3301 aatgtctgag tcgaggatca tcaattccgg cttgccatcc aggctcactc ttagtaactt
     3361 ttgcccgcga atgatgagga gattaagaat gctgaagaat ctggctaaac tggatcaaac
     3421 agaaatggat aaagtgaatg tcgatttggc ggcggccggg gtggctttta aagaacgcta
     3481 caatatgccg gtgatcgctg aagcggttga acgtgaacag ccagaacatt tgcgcagctg
     3541 gtttcgcgag cggcttattg cccaccgttt ggcttcggtc aatctgtcac gtttacctta
     3601 cgagcccaaa cttaaataaa acttatacag agttacactt tcttacataa cgcctgctaa
     3661 attatgagta ttttctaaac cgcccccata atttgcagtc attttgaaaa ggaagtcatt
     3721 atgtcgttct ggaaagttgc agcagcgcaa tatgagcctc gtaaaacctc actcaccgaa
     3781 caggtagctc atcatctgga gtttgtgagg gccgccgcca ggcaacaatg tcagctgtta
     3841 gtttttccat cgctttcttt actggggtgt gattattcgc gacgtgccct tcctgcccca
     3901 cccgatcttt cactgttaga cccgctttgt tatgccgcaa cgacctagcg aatgaccatc
     3961 attgccggcc ttcccgttga atataacgat cgctttattc gtggaattgc ggtgttcgca
     4021 ccgtggcgaa aaacgcctgg gaactaccat cagagtcatg gtgcatgtct gggccgtcgt
     4081 tccagaacca tcacggtggt cgatgaacaa ccgcaaggta tggatatgga cccgacctgt
     4141 tcactgttta cgacagggca atgtctggga gaacccgacc tgctggcgtc ggcccgtcgc
     4201 ttacaatttt tttcacatca gtactcgatt gccgtgctga tggcaaatgc ccgtggtaac
     4261 agtgcactat gggatgaata tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg
     4321 ttagtcggtc agcgttcatc acagggttgg caaggcgata tcattccatt acgctaggct
     4381 ttttcggcct ggagcatgcc atgttgcgca ttatcgatac agaaacctgc ggtttgcagg
     4441 gagggatcgt tgagattgcc tctgttgatg tcattgacgg aaaaatcgtc aaccccatga
     4501 gcctcctggt gcgccccgat cgtcctatta gtccacaagc gatggcgatt catcgcatca
     4561 ccgaagccat ggtcgccgat aaaccgtgga ttgaagatgt gatcccacac tattacggta
     4621 gtgaatggta tgtcgcgcac aacgccagct ttgaccgccg cgtactgcct gagatgcccg
     4681 gtgagtggat ttgcactatg aaactggccc gtcgtttgtg gccagggctc aagtacagca
     4741 atatggcgtt atataaaaca cgcaagctca atgtacagac accgccgggc ctgcatcatc
     4801 accgcgcgtt gtatgactgt tatatcaccg ccgcgttgct tatcgatatt atgaacacct
     4861 ccggctggac ggcagaacag atggccgata tcaccggacg tccgtcgttg atgacgacct
     4921 tcacctttgg caaataccgt ggcaaagcgg tttccgacgt tgccgaacgc tatccgggct
     4981 atctgcgctg gttatttaat aacctggaca gcatgagccc ggagctgcgt ttaacactga
     5041 aacattatct ggaaaatact taatccgcag gcgtagcggg taatgttccc tgcgccagcg
     5101 cgaccagaaa agcatattcc atcgctacgc cttcgtacga tttaaagcgg ccagatttac
     5161 cgccatggcc tgagtccatg tcggtacaga gcaataaaag atggtcatcg gttttcagct
     5221 cgcgcaattt agcgacccat tttgccggtt cccaatattg cacctgagaa tcgtgtaaac
     5281 cggtcgttac cagtaaatgc ggataagcct gtgcggtgac gttgtcatac gggctgtagc
     5341 ttttcatgta ctcgtaatat tgcggatcct gcggatttcc ccactcttca aactcaccag
     5401 tggtaagagg aattgattca tcaagcatcg ttgttacaac atcaacaaac ggtacctggg
     5461 cgataacgcc gtggaataat tccgggcgtt gattaattgc aacgcccatc aacatgcccc
     5521 ccgcacttcc gcccatcgca taacagagcg aaggagagcc atagcccagt tttaacaatg
     5581 catcgcaggc atcaagataa tcattaaacg tatttttctt cttcagaaat ttaccgtctt
     5641 cgtaccattg ttgccccagc tcaccaccgc cgcgaacatg gacaatggcg tagacaaagc
     5701 cacgatctaa caaactcaag cggctaaaac tgaaatcggc atcaatactt gcgccgtaag
     5761 aaccatagcc atacaccagc aacgggttgt gtcctttgcg aaaatgtttg cgatgataga
     5821 ccagcgaaac cggaacttcg acgccatcac gggcgactat ccacaggtgt tcactgcggt
     5881 aattcgccgc atcaaaacca ggcacttctg tttgttttaa tacacgacgc tcaccggtat
     5941 ccatatccag ttcaaacaaa gtgtctggtg tggtcatgga agaataacca taacgcaatc
     6001 gcgcggtttc aggttctgga ttgtaggcaa tccaggtcac ataggccgga tcatcaaagg
     6061 caatgccaat gacttcccgg gtcttgcggt taatttggcg caaactggtt aacccgcgct
     6121 gacgctcttc aaccaccagc cagtcggtaa acagcgtaaa cccttccagc atgatgtttt
     6181 cgcgtggcgg aattaactct tcccactgtt gctcatcacg catacgggta cggtataagc
     6241 caaagttttt gccgtggcga ttggaacgca gataaaaacg atgctggtag tgatcaaggc
     6301 tgtattcgtg atctttgcgg cgcggcagaa aaacaaacgg ctcggcatcg gccatttccg
     6361 cgtccagcag gcgaacttca ctggtggtgg cgctggccag atgaatgacg acatagtgct
     6421 tcgacgtcgt tttatgcagg ctgacataat aggtatcgtc tttttcttcg tagatcagtg
     6481 tatcttgcga tgctggcgta ccgatggcgt gacgccagac ctgataaggc agcagcgtca
     6541 ccggatgctt gcgaacatag tagaaagtcc aggagtcatt tgcccagaca aagctgggtt
     6601 caacgttatc cagcagttcc gggtaccagt tacctgtttc cagattacga aaacgaatgc
     6661 cgtactggcg tcgggaaaga aaatcttctg ccagcgccat aatggtgtta tcgggcgtaa
     6721 tcgccattcc gcccatcgaa taaaactcac tatgagctgc gcgcttgttg gcatcgagca
     6781 atgtttccca ctcatcccac tcttcgctga atgccgattg acgctggtag atagcatatt
     6841 cacagcctgg ttcataaata tgccgatagc ggtagccatt ttttatgtag ggcgcagaaa
     6901 cttctctttg cggaatgcgg tcgatgattt cctttaagat gcgatcctgc aaggcttgtt
     6961 gtgaggccat cacccgatga ccgtaactat tttcttgttg caggtagtcc aggacttttg
     7021 gctgagaacg cgtatcgtcc cgcagccagt agtaattatc gatgcgcgta tcgccatgaa
     7081 gcgtcatggc gtggggaatg cgggcggctt ttggtagcat gttattgttc tttctggttg
     7141 gaaacatctt ataagggtgg caaaactcac cggggatgca agcgaaacag ggtcgtcatt
     7201 gcttagatga tgacaggtaa tggcgcggat atcgaatgtt atgcagacag agaaaatcag
     7261 cctgttcaaa tggctgtgcg gttctggata gcccgaaata gtcaacttca ggctatccag
     7321 agagcggaat tattccgcca aagtgcgttt ttgctgttcg agatcgcgtt caatgccttc
     7381 acgaacatcc tgggggattt tcagcgcgtc acccagtgca ttcaggtaac tgcgttccat
     7441 aaaatggtca atatcaatag ccgcgcagct caggaaatag atttccagcg cctcttcttc
     7501 attgcggacc ccggtagcca ggcgttgtgg atccagcggt tgttcgattg cctgctcaat
     7561 gagtacacgc ccctgctctt ccacgccggc ttcacgcaat tgctggtcga ttgccgcacg
     7621 ttcattggca tcaatatgac catcactttt agcggcaaag accagcgcaa ggatcaaacg
     7681 ttccgtacgc tcatccagcg gcgtactttg cgcgccaaac tgcggttcgt cctgatgcgc
     7741 cgcgcgaatt ttatctttgt atttattcca cagcaccgta cccgctaccg ctccgccgcc
     7801 aaccagtaac gcgttggtgc catatttcgt aagaagttta cgtgctgatt tatttgcgac
     7861 cagcagccca gccagtccgc ctaatgcgcc tgacactaac agtttgccca atccctgatc
     7921 cgcagacgag gaggtagaag aactgctttg cccaagcagg gattgcagtt gatttaacca
     7981 gttagccata tttgctcctc aataaccatt tttagtgcct gtagcatagc ggatgaggat
     8041 gtcagaaaat gtatatcgag acaaaagatg cgcaaataag cggggcggta ggccgttatt
     8101 caaagaaatt cgcgccaggt aaggtattta ctgtatatca agtgataagg gtattttagg
     8161 tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct gattgcccga tgcgacgctg
     8221 ttgcgtcttc tcaggcctac gattgtggtg cgttctggga cggacaaggc actcgcgccg
     8281 catccgccaa cggtcaatcg aattatcaga agccattaac gccgctgata ttccgccatt
     8341 cccgctttgc agtccacgct gacctggtaa tgaatatcgg cacttttacc acgcacggtt
     8401 agcggtactg accatttatc atctttaccc tgaatgtcct gcaaactgac ccaggccacg
     8461 ggatcggcct gaccgacaat tttttgatca tctgcccagc gcgccacgcg attttgttga
     8521 taatcacgtt ttacgctcgc ggcaattccg gcagcatcca gaccttcaca ctttgggaaa
     8581 gtgaccgact tgctggtttc attattggca gcgaaaactg atgcgcaggc agaaaccaac
     8641 aaccgcccta aaaacgcccc tctttttttc atgtttttct ccatagcaca atgattcagg
     8701 agaaagcatg gtacaaattg tcaggagcgc aagttgcttc aggccgcgtg tgaggcatct
     8761 tcctcttcgg attcagcacc gacgacctgt ggggacggta atttgccggt tttcagaatg
     8821 gtgctcagca cctctttttg ttctgccagc catagcgaaa gggcttcacg ttgctcgtct
     8881 tccatttgca ctggagaatt tgtcagctag gtgtcaagca gatccgccgt atcaagcatt
     8941 ttgtcataag catcggcttc ctttttgctg gtaaacgaca ttttctcttc gccctcacga
     9001 atgactacgt atttaacttc aaccgccatt tgcagcctct cataataact gagattttat
     9061 acagtatatt tcttttcggt tgagaaatca acatcagcaa taaagacaca cgcaaacgtt
     9121 ttcgtttata ctgcgcgcgg aattaatcag gggatattcg ttatgacgtt attaggcact
     9181 gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa
     9241 gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg ccgtcgatcg ctatgccgac
     9301 gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta atatgcttga tggtgatgca
     9361 ttacgccgtg tggttgaact ggaaaaacca cattatatcg tgccggagat cgaagctatt
     9421 gccaccgata tgctgatcca acttgaagag gaaggactga atgttgtccc ctgcgctcgc
     9481 gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc tggcggcaga agagctgcag
     9541 ctgcccactt ccacttatcg ttttgccgat agcaaaagcc ttttccgcga ggcggttgcc
     9601 gccattggct atccctgcat tgtaaaaccg gtgatgagct cttccggcaa ggggcagacg
     9661 tttattcgct ctgcagagca acttgctcag gcatgggaat acgctcagca aggcggtcgc
     9721 gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt ttgacttcga aattaccctg
     9781 ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac cagtaggtca tcgccaggaa
     9841 gatggcgact accgtgaatc ctggcaacca cagcaaatga gcccgcttgc ccttgaacgt
     9901 gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg gttatggtct gtttggtgtc
     9961 gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg tctcccctcg tccacatgat
    10021 accgggatgg tgacgttaat ttctcaagat ctctcagagt ttgccctgca tgtacgtgcc
    10081 ttcctcggac ttcctgttgg cgggatccgt cagtatggtc ctgcagcttc tgccgttatt
    10141 ctgccacaac tgaccagtca gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca
    10201 gatttgcaga ttcgtttatt tggtaagccg gaaattgatg gcagccgtcg tctgggggtg
    10261 gtactggcta cttcagagag tgttgttgac gccattgaac gcgcgaagca cgccgccgga
    10321 caggtaaaag tacagggtta aacccggcaa aaaaacgcta caaaaatgcc cgatcctaga
    10381 tcgggcattt tgacttttac agcttagcgc cttctacagc ttcacgcgcc agcttagtaa
    10441 tgcggtcgta atcgcccgct tccagcgcat ctgccggaac cagccaggaa ccaccgatgc
    10501 acagcacgct tttcagtgcc aggtagtcac ggtagttagc cggagaaata ccacccgtcg
    10561 ggcagaaacg gacctgggag aacggacccg cgatcgcctg cagggctttc acgccgccgt
    10621 tagcttcagc cgggaagaat ttgaactctt tcaaaccgta gtccataccc agcatcagtt
    10681 cggaaacagt gctgatcccc ggaatcagag gaatagtccc ttcggtagca gctttcagca
    10741 gcggctcggt cagacccggg ctaattgcga actgtgcacc cgcttcagtg acttctgcca
    10801 gctgctgtgg attcagcacc gtaccggcac ccacaatcgc ttcaggcact tctttggcga
    10861 tagcacggat agcgtcaact gcacactcgg tacgcagagt cacttccaga acgcgcaccc
    10921 caccagcaac caacgctttt gccatcggca ccgcgtgttc cagttttttt accacgataa
    10981 ccggtacaac cgggccggtg gtcaggattg attctgcact tgttttccag tttttcatca
    11041 gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa agtgatacag gttgcgccct
    11101 gttcggcacc ggacagtttt tcacgcaagg cgctgaataa ttcacgtcct gttcccacgc
    11161 gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc cagttccgct tcgtctacca
    11221 gcagtgtcag ttcgcctgtc tgtccattca cacgaatgat gtccccgtcg cgcacttttg
    11281 ccagcagccc gccatcgtag gcttctggtg ttacgtggat agctgacggc actttacctg
    11341 aagcgccgga gagtcgtcca tcggtaacta acgcaatttt gaaacaccgg tccaataata
    11401 caccaagtgg cggcatgagt ttatgtaatt ctggcattcc gttcgctttt ggcccctgat
    11461 gacggacaac aacgacacaa tcgcggtcca gcaaacccgc ttcaaaggcc ggcataacgt
    11521 catgctggct ttcaaaaaca accgctggcg cttcaatcac ctggttctca accggcacgg
    11581 cagaggtttt cataaccgca cggcccaggt taccgcttaa cacttttgtc ccaccatgat
    11641 gagagaaagg ttgttcgaag gaagcgatca cattgctgtc gagtgatttt tccgcccctt
    11701 cccgccagtc cagttcacca ttattcagcc atggttcaag ggtataacga gacagaccaa
    11761 agcctgccac cgtattgaca tcttcatgca gcaggcctgc tttgagcagt tcacgcacca
    11821 gaaccggtac gccacctgcc gcctggaagt ggttaatatc ggccggaccg ttcgggtaga
    11881 gacgtgccat cagcggtaca acatcagaaa ggtcagagaa gtcatcccag ttaatctgaa
    11941 taccggccgc gcgcgccatc gccaccaggt gcatggtgtg gttagtggaa ccaccggtcg
    12001 ccagcagtgc aacgataccg ttcaccacca ctttctcatc gatcatctta ccgatcggca
    12061 tccattcatt accattaccg gtcatgcgtg taacctgacg cgcagcggca gcggtcaaag
    12121 catcgcgcag cggagaatcc ggatgaacaa aagaagagcc tggcaactgc atccccataa
    12181 attccaccac catctggttg gtgttggcag taccgtagaa agtacatgtt cccggcgcat
    12241 ggtaagacgc ggcttctgac tccagtaagg ccatgcggtc cactttacct tcggcataaa
    12301 gctggcgaat acgcactttt tctttatttg gcaaaccgct tgccatcggt ccagacggca
    12361 caaacaccgc aggcaaatga ccaaacgcca gggctgccat cgtcagaccc gggacaatct
    12421 tgtcgcacac accgaggaac agagcaccat caaacatgtt atgggacagc cccaccgccg
    12481 cagacatcgc tatcacttcg cggcttagca gcgacaattc cattccatcc tgcccctggg
    12541 tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg accaaccgca ttcgcttcat
    12601 gcagggcttt acgaatgatt tctggatagt gttcataagg ctggtgcgcg gagagcatgt
    12661 cgttatagga ggtgatgatg gcgatattgt tacgcaacat gcttttcaaa gaggctttgt
    12721 cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc gcatgccaac tgcgaacgat
    12781 gaacggtcga cgttttcgct tgttctatcc gggcgagata agcagagcga gtctcgcgcg
    12841 aacgttcaat gattcgattt gttacgcgta acaattgtgg attcataaag gctcctgaaa
    12901 ttgagttgtc agagcaggat gattcacaac gcgtttcatt cagaggattt atgagtgaaa
    12961 cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc agtgtaataa aaaaagcctc
    13021 gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt tctacaatct gtgcaagatc
    13081 atgttaccgg taaaataacc ataaaggata agcgctgata ttactcaaac tcattccagg
    13141 aacgaccatc acgggtaatc atcgccaccg aggcaacggg tccccaggtt ccggcctgat
    13201 acggtttcgg cgcatcattg tccatcgccc acgcctcagt aatggagtct acccatttcc
    13261 aggcttcttc cacttcgtcg cgacgtacaa acagtgcctg aataccacgc atggtttcca
    13321 gcagcaaacg ttcataggca tccgccagat gcgtctgatt aaaggtttct gaatagctca
    13381 gatccagctt ggtgatttgc aggttatgtt tgtggtcaag gccaggaact ttattcagta
    13441 cctggatatc cacgccttca tcaggttgca gacggatagt cagtttattc tgcggcagat
    13501 cctgccacga ctctttaaac agattcagtt caggtgtttt gaaatagacc acgacttcag
    13561 aacatttggt cggcagacgt ttaccagtac gcaggtagaa tggcacaccg gcccagcgcc
    13621 agttatcaat gtcgacgcgg atcgccacga aggtttctgt attgctgctt ttgttcgcgc
    13681 cctcttcttc cagatatccc ggcacttttt tgccctgggc gaagcccgca gtatattgcc
    13741 cgcgtacggt tttttcgcgt acgttggagc ggtcgatgcg gcgcagggac ttcagtactt
    13801 tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga tggcggagac atcgcaatca
    13861 tgcagagaat ttgtagcagg tggttctgaa tcatgtcacg catctgaccg gctttatcaa
    13921 aatagcccca gcgcccttcg atccccactt cttctgccac ggtaatctca acatgatcaa
    13981 tggtgcgatt gtcccagtta ttcacaaaca gggagttagc aaaacgcagc gccaacaggt
    14041 tcagcaccgt ttctttacca agatagtggt cgatacggta aacctggcac tcctcgaagt
    14101 attcgccaac ctgatcattg atttcctgcg aggtcgccag cgacgtcccc agcggtttct
    14161 ccatgactac gcgtgccggt ttagcattca gttttgcctc gccaagccct ttgcaaattg
    14221 cgccaaaagt gctgggcggc atggcaaagt agttaatagt gatacgattt ttttgatcca
    14281 gcatcgcgcc gagacggttg aatgcagcag tgtcattgac atcgagatta caaaaatcca
    14341 gacgtgcact cagggtgtcc cataaacctt catcaatggt ttctttcatg aaagtttcga
    14401 gcgcctcgcg gacaactttg gtatatgccg ctttatccca gtcagcacgc cctacgccga
    14461 taatccgggt gtccgggttg agctgaccga ctttttccag ttgatacagg gaaggcagca
    14521 atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac caggtcacag gcctgggctg
    14581 tttgcgttac cgccatgtca ttctccttaa gttaactaac ccggtactta agccagggta
    14641 tacttgtaat tttcttacgg tgcactgtac tgcttttacg agcttgcgaa aactgtaaac
    14701 gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagt gcggcaaaac tgataaaaaa
    14761 atcatcgttt tccattcggt aaaaatctga cactgatcat gttatgaaaa aaaataacaa
    14821 cttttttatc tgcttttgtc attaacacgg cacacaggcg taatatctga caaaactgca
    14881 tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga gtctctttac atcatgaata
    14941 tgctggaaaa aatccagtct cagctggaac atttgagcaa atcagagcgc aaagttgccg
    15001 aggtcattct ggcttcgccc gataacgcga tccattcgag tattgctgct ctggcgctgg
    15061 aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg cagcatggac acgcgcggtt
    15121 ttcctgattt taaacttcat ctggcacaaa gtctggcgaa tggcactccc tatgttaatc
    15181 gcaacgtcaa tgaagatgac agcgttgaat catacacggg gaaaatattt gagtccgcaa
    15241 tggcaacgct cgatcatgtc cgtcattcac tggataaatc tgccatcaac cgcgccgtcg
    15301 acttgctcac tcaggcaaaa aaaatcgcct ttttcggatt aggctcttct gccgccgttg
    15361 cccacgatgc gatgaataag ttctttcgtt ttaatgttcc ggtggtgtac tccgatgata
    15421 tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg agacgtggtg gtgctgattt
    15481 ctcacacagg aagaacaaaa aatctggtcg agctggcgca gctggcacgc gaaaacgacg
    15541 ccatggtgat tgccctcacc tctgcgggta ccccgctcgc ccgggaagca acgctggcaa
    15601 ttaccctcga cgtaccggaa gatactgaca tttatatgcc catggtttct cgacttgcac
    15661 agctgaccgt gatagatgtg ctggcgacag gatttacttt gcgacgcggt gcaaaattca
    15721 gagataactt gaagcgggtc aaggaagcgc tgaaggaatc gcgttttgat aagcagttac
    15781 ttaatttaag tgacgatcgc taaaagcgac tgtcactgtc ctaatcttat acgacatccg
    15841 aatgagatta atttatcgcc atcgcggcgt tatttcattc ggatttcatg ttcaagcaac
    15901 acctggttgt ttcagtcaac ggagtattac atgtccaaaa ggcttcgcag aacaaaaatc
    15961 gttaccacgt taggcccagc aacagatcgc gataataatc ttgaaaaagt tatcgcggcg
    16021 ggtgccaacg ttgtacgtat gaacttttct cacggctcgc ctgaagatca caaaatgcgc
    16081 gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc atgtggctat tctgggtgac
    16141 ctccaggggc ccaaaatccg tgtatccacc tttaaagaag gcaaagtttt cctcaatatt
    16201 ggggataaat tcctgctcga cgccaacctg ggtaaaggtg aaggcgacaa agaaaaagtc
    16261 ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg gtgacatcct gctgctggac
    16321 gatggtcgcg tccagttaaa agtactggaa gttcagggca tgaaagtgtt caccgaagtc
    16381 accgtcggtg gtcccctctc caacaataaa ggtatcaaca aacttggcgg cggtttgtcg
    16441 gctgaagcgc tgaccgaaaa agacaaagca gacattaaga ctgcggcgtt gattggcgta
    16501 gattacctgg ctgtctcctt cccacgctgt ggcgaagatc tgaactatgc ccgtcgcctg
    16561 gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg ttgaacgtgc ggaagccgtt
    16621 tgcagccagg atgcaatgga tgacatcatc ctcgcctctg acgtggtaat ggttgcacgt
    16681 ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg gcattcagaa agcgttgatc
    16741 cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga cccagatgat ggagtcaatg
    16801 attactaacc cgatgccgac gcgtgcagaa gtcatggacg tagcaaacgc cgttctggat
    16861 ggtactgacg ctgtgatgct gtctgcagaa actgccgctg ggcagtatcc gtcagaaact
    16921 gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa tcccgagcat caacgtttct
    16981 aaacaccgtc tggacgttca gttcgacaat gtggaagaag ctattgccat gtcggcaatg
    17041 tacgcagcta accacctgaa gggcgttacg gcgatcatta ccatgaccga atcgggtcgt
    17101 accgcgctga tgacctcccg tatcagctct ggtctaccaa ttttcgccat gtcgcgccat
    17161 gaacgtacgc tgaacctgac tgctctctat cgtggcgtta cgccggtgca ctttgatagc
    17221 gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc tgctgcgcga taaaggttac
    17281 ttgatgtctg gtgacctggt gattgtcacc caaggcgacg tgatgagtac cgtgggttct
    17341 actaatacca cgcgtatttt aacggtagag taagtacgtt gccggatgcg gcgaaaacgc
    17401 cacatccggc ctatagttca atgatagttc aacagatttc gaatattctg aagcaaactt
    17461 gaacttatca tcaggcgaag gcctctcctc gcgagaggct tttttatttg atgggataaa
    17521 gatctttgcg cttatacggc tggatttcgc ccggtttgcg agttttcagc aattttagta
    17581 tccaggtgta ttgttctggt cgcggaccaa caaaaatctc gacttcttca ttcatccgcc
    17641 gctcaatcgt atgatcatcc gcctctaaca gatcatccat cggtgggcgc acctgaatcg
    17701 tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg tacaacgcgc gcacggcata
    17761 ctttcatcaa acgaccaatc gcgggcaacg tcgctttata ggtggcaaag aaatccacaa
    17821 attcgctgtg ctctgggcca tgatcctgat cgggtaaata atatccccag tacccctgac
    17881 gtaccgactg gatgaatggt ttaataccgt catttctcgc atgcagacga ccgccaaagc
    17941 gacgacgcac cgtgttccag acataatcaa aaaccgggtt gccctgatta tggaacatcg
    18001 ctgccatttt ctgcccttgc gaggccatca gcatggcagg aatatcgacg gcccaaccgt
    18061 gcggcaccag aaagataact ttctcgttat tacgccgcat ctcttcgatg atctccagcc
    18121 cttgccagcc aacgcgcggc tgaattttct ccggcccgcg tattgccaac tcagccatca
    18181 ttgccatcgc ttgcggcgcg gtggcaaaca tctcatcaac aatcgcttcg cgttcagctt
    18241 cactacgttc tggaaagcag agcgacagat tgattaacgc acgacggcgt gagctttttc
    18301 ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg atcacggaac tttggcggcg
    18361 ttaaagcgat acccgccatc gctgctacac ccagccatgc tccccagtag cgcgggtggc
    18421 gaaaggattt atcaaactca ggaatgtatt cgctattatt ttttttcgtt tccatgcttt
    18481 tccagtttcg gataaggcaa aaatcaatct ggtgatagtg tagcggcaca acttgccccg
    18541 caccaaataa aaaaagccgg tactgactgc gtaccggctg cgaatggatg ttaattaatc
    18601 aaaccgtagc tgcggcacaa tctctttggc ctgcgccagg aattcgcgac gatcggagcc
    18661 ggtcagccct tcggtacgcg gcagttttgc cgtcagcggg tttacggcct gctggtttat
    18721 ccatacttca tagtgcagat gcggcccagt tgaacgtccg gtattaccgg aaagcgcgat
    18781 acggtcgcca cgtttcacct tctgtcccgg tttcaccaga atcttgcgca agtgcatata
    18841 acgcgtggtg tagctgcgac catgacgaat agccacataa taacctgctg cgccactgcg
    18901 tttggcaacc accacttcac cgtcacccac tgaaagcact ggcgtacctt gtggcatggc
    18961 gaaatcaaca cctctgtgtg gtgcaacgcg acctgtcacc ggattagttc gacgcgggtt
    19021 aaagttagac gagatacgga actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag
    19081 accagtaccg ttacgatcgt agaatttgcc atcctcagcg cggattgcgt aataatcttt
    19141 accttctgaa cgcaaacgta cgcccagcag ctggctttgc tcacgtttac catcaagcat
    19201 ttctcgagac attaataccg caaattcatc gccttttttc agtttgcgga aatccatttg
    19261 ccactgcatg gctttaatca ctgcgctcac ttcggcgctg gttaaaccgg cgtttctggc
    19321 gctggcaaca aagctccctc cgacggtacc tttcagcaga ttgttaaccc actctccttg
    19381 ctgcatttcg ctggtcattt taaaaccgtt agcggcagta cggtcatagg ttcgggtttc
    19441 acgacgagac acttcccagg tgaggcgctg cagttcgccg tccgcggtta atgtccagga
    19501 gagttgttga ccgattttca ggttacgcaa ttctttgtcg gcagcagcaa gttgggtgat
    19561 atcacccata tcaataccat actgattgag aatgctgctt agcgtatcgc cagtggaaac
    19621 aacatattca tgcacgcccg cttcaccggc gattttgtca tccagttcgt cctggggaat
    19681 ggcttcatct tcttgtgcag cttgatcaat cggctcactg gcttcaggta agagcgaacg
    19741 aatttcgttc tgttccagct caatggtttt gacaattggc gtggcatcac ggtgataaac
    19801 atagggccgc cagacagcga cggccagagt aagaacggtg agcgacccca acataacgcg
    19861 gtgtggtcgc ggtaaattat taaacgccag ggcgacagag cgggctatct gttgcacgta
    19921 atcacttcct cattaatctc ctttcaggca gctcgcatac tggttggcta attgactcag
    19981 gaattctgaa tagcttgttt tacccagttt gatattcgtc cccaggggat ccaacgttcc
    20041 catacgaacg gatgtccctc gtgcgacgct ctcaacgacc gctggcctga actgtggctc
    20101 agcaaaaacg caggttgctt tttgctcaac caactgtgtt cttatttcat gtaaacgctg
    20161 cgcgccaggt tgaatctcag ggttaacggt aaaatgacca agcggtgtca gtccgaactg
    20221 tttttcgaaa tagccgtaag catcgtgaaa aacgaaataa cctttcccct tgagcggcgc
    20281 gagctcgtta ccaacctgcg tttcggttga ggccaattgt gcctcaaaat ccttcaggtt
    20341 ggcgtcaagt ttggctcgac tttgcggcat aagttccact aattttccat ggattgcaac
    20401 cgctgtagcc cgcgctatct ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg
    20461 gtgatcttcg tcactttttt ccgcgtggtc gtgatcatca tcatcgccgt gaatactttt
    20521 catcagtagc ggtttcacat cttcaagctg cgcaatcgtt acctgcttcg cttctggtaa
    20581 tttgcttacc ggtttttgca taaacgcttc catctccggg ccaacccaaa cgactaagtc
    20641 cgcgttctgt aagcgtttta catccgatgg acgcagtgaa taatcatgtt ctgaagcgcc
    20701 gtcaggcagt aaaacctccg tttctgttac accatcagca atggcagaag cgatgaaccc
    20761 aacgggttta agcgaagcga caacggcggc atctgcggcc tgtgttgcac ctccccagag
    20821 agcggcggat aatgctgcga aaagaagcgt ttttttatgt aacataatgc gaccaatcat
    20881 cgtaatgaat atgagaagtg tgatattata acatttcatg actactgcaa gactaaaatt
    20941 aacatgacaa gtctggtttc cctggaaaat gtctcggttt cttttggcca acgccgcgtc
    21001 ctctctgatg tgtcgctgga acttaaacct ggaaaaattt tgactttact tgggccaaat
    21061 ggcgcaggta agtcgacact ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg
    21121 gttatcaagc gcaacggaaa actgcgcatc ggctatgtac cgcagaagct gtatctcgac
    21181 accacgttgc cactgaccgt aaaccgtttt ttacgcttac gccctggtac acataaagaa
    21241 gatattttgc ctgcactgaa acgtgtccag gccgggcacc tgattaacgc accgatgcaa
    21301 aagctctctg gtggcgaaac gcagcgtgta ctattagcgc gagcattgtt aaattgcccg
    21361 caattattag tgctggatga acccactcaa ggcgtggatg taaatggcca agtggcgtta
    21421 tatgacctta ttgaccagtt gcgtcgtgaa ctggattgtg gcgttttaat ggtttctcac
    21481 gatctgcatc tggtgatggc aaaaaccgat gaagtgctgt gcctgaatca ccacatttgt
    21541 tgttccggca caccggaagt tgtttccctg catccggagt ttatttcaat gtttggtcct
    21601 cgtggtgctg aacaactggg tatctatcgc catcatcata atcatcgtca cgatttacag
    21661 ggacgaattg ttttgcgtcg gggaaatgat cgctcatgat tgaattatta tttcccggtt
    21721 ggttagccgg gatcatgctc gcctgtgccg cgggtccgct gggttcgttt gtagtctggc
    21781 gtcgtatgtc ttatttcggt gatacgctgg ctcatgcctc attacttggt gtcgcgtttg
    21841 gtttgttgct ggacgtgaat ccattctatg cggtgattgc cgttacgctg ctgctggcgg
    21901 gcggtctggt atggctggag aagcgtccac agctggcgat cgacacgtta ttagggatta
    21961 tggcgcacag tgccctgtcg ctgggcctag tggtcgttag tctgatgtct aacattcgtg
    22021 ttgatttgat ggcttacctg ttcggtgatt tactggcagt gacgccagaa gatctcatct
    22081 ctattgcgat tggcatggtc atcgtggtgg ctattttgtt ctggcaatgg cgcaatttgc
    22141 tgtcgatgac gattagcccg gatctggcgt ttgttgatgg tgtgaaatta cagcaagtga
    22201 aattgttgtt gatgctggtg acggcattga cgattggtgt agcgatgaaa tttgtcggtg
    22261 cgttgattat tacttcgttg ctgattattc ctgcagctac tgcgcgtcgc tttgcccgta
    22321 cgccggaaca gatggctggt gtcgctgttt tggtggggat ggtggcagta actggcggtt
    22381 taaccttttc cgcgttttac gatacgccgg cgggtccgtc ggtggtgcta tgtgcggcac
    22441 tgttatttat tatcagtatg atgaaaaagc aggccagcta atctatcgct gaacacattt
    22501 gtcggatgcg gcgcgagcgc cttatccgac ctacggttcg ctatctctgg ctggcctgat
    22561 aagacgcgaa cagtgtcgca tcagacacac tgccagtgtc ggatgcggcg cgagcgacca
    22621 atccgactta cggcatttct ggcggcgtta tgccaaagtg attccacgcc cgcgtcgtcg
    22681 ccatacgccc gcgcggtgta cgctgcaaaa agccttgctg aatcaaataa ggttccagta
    22741 catcttcaat ggtttcacgt tcttcgccaa tggctgccgc caggttatcc agacctaccg
    22801 gcccaccaaa gaacttatcg attaccgcca gcaacaattt gcggtccata taatcgaaac
    22861 cttcagcatc gacattcaac atatccagcg cctgagcagc gatatctgcc gagatggtgc
    22921 catcgtgctt cacttcggcg aaatcacgca ctcgacgcag cagacggttg gcaatacgtg
    22981 gcgtaccgcg agcgcgacga gctacttcct gcgcaccgtc atcactcatc tcaagcccca
    23041 taaagcgtgc actgcgactg acgatatatt gcagatccgg cacctgataa aactccagac
    23101 gttgcacaat accaaaacgg tcgcgcaacg gtgatgtcag cgaacctgcg cgcgtggttg
    23161 cgccaatcag ggtaaacggc ggcaaatcaa ttttaatgga gcgtgccgcc ggaccttcac
    23221 caatcatgat atccagttgg tagtcttcca ttgccgggta cagcacttct tcaacaactg
    23281 gcgataaacg gtggatctca tcaataaaca gcacgtcatg cggttcaagg ttagtgagca
    23341 tcgcagccaa atcgcccgcc ttttccagca ccggaccaga agtcgtgcgt aaattaacgc
    23401 ccatttcatt ggcgacaatg ttggcaagcg tagttttacc caaccccgga ggaccaaaaa
    23461 tcaacaaatg atcgagggca tcgccgcgca gtttcgctgc tttgatgaaa atctccatct
    23521 gtgaacgaac ctgcggctga ccaacatact cttccagtaa tttagggcga atggcgcgat
    23581 ctgctacatc ttccggcaaa gtggtaccgg cagaaatcag acggtctgct tcaatcatcc
    23641 tttacctcat aacgcggcgc gtagggcttc gcgaattaat gtttcactgc tggtgtcagg
    23701 gcgagcgatt ttgctcacca tgcggcttgc ttcttgtggt ttatagccta gcgccaccag
    23761 cgcggcaact gcttcttgtt cagcatcgtc ggtcgccggg ctagcaggag acgtgagtac
    23821 caggtcggcg gctggcgtaa agagatcgcc atgcaaacct ttaaatcggt ctttcatttc
    23881 aacaatcaag cgttcggcgg tttttttgcc aatacccggc agtttcacca gtgcccccac
    23941 ttcttcacgc tcaacggcat taacgaactg ctgcgctgac attccggaga ggatcgccag
    24001 ggccaacttc gggccgacgc cgttggtttt gatcaactct ttgaacaacg tgcgttcttg
    24061 tttattgtta aaaccgtaca gcagttgcgc gtcttcacgc accacaaagt gggtgaaaac
    24121 gatcgcttcc tgacccgctt cagggagttc ataaaaacag gtcatcggca tatgcacttc
    24181 atagcctacg ccgcccactt caattaacac cagcgggggt tgtttttcaa tgatgatgcc
    24241 tctgagtctg cctatcacat gacgctcctg cgtaatgaat caaagataat gctgtatgat
    24301 aaaaaaatgc tggatagata tccagcgaag gatgaagaaa acttgcgagg tgtcccgatg
    24361 atctgaaaaa tggcacagta taatttattc aacaggttat attggaagca aatatttgaa
    24421 tattacatat tcagtgaaga aatgcgtaat aaaaatatac attgcgactc ctgaaaaata
    24481 atttttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
    24541 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctacctta
    24601 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
    24661 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
    24721 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
    24781 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
    24841 ctttaccaca cgggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgtcttt
    24901 gatatttata aagaggcgct atgtattccg gtgggtgaaa tagagacgtt tggacaactg
    24961 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
    25021 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tttggtgttg
    25081 attcacgcgg agggagttga aacgcctcag ccggaactga ccgaggcggt ataacttaac
    25141 gcagtcgccc tctcgtcagg ttcagccgcg attcgctcat ctgcatcgca ttctgactaa
    25201 cgtggcagtg ggtgatggca atcgccagcg catcggcggc atccgcctgt ggattagcgg
    25261 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctacccatac
    25321 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
    25381 ccgccgcaac aatcgccacg ccgcgcgcct ggcccagttt cagggctgag tcagcgttct
    25441 tcgccataaa gacttgttca atggcgaaat aatcaggctg gaactgggtg atgatttccg
    25501 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
    25561 atccgctacc taggtaggac agttgcctac ctacctggcg gatgacgccg tagccggtca
    25621 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
    25681 agcaggcatc atcagagagt cgctgcaacc tcatcagaga tttcaccgtt atggtaaact
    25741 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggcgcggtt
    25801 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
    25861 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagaca
    25921 tcaatcgcgc cgtcatcata tgtcacaacg tcttcagcac ctgcttccag agctgcttcc
    25981 atgatggtgt cttcgtcgcc tttctcgaag gagatcacgc cttttttgct gaacagataa
    26041 gctacggaac cgtcagtgcc gaggttaccg ccacatttgc tgaatgcgtg acgcacttca
    26101 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    26161 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    26221 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    26281 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    26341 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    26401 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    26461 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    26521 cgccgccgca acaggcgcat caagccactt gtaagccaga tgttcagtga aaacgatctg
    26581 ccgctcgtgc ggaagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    26641 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    26701 ggtcagttgt tcagcgacaa catcaatggt aacctcttcc tttacttcgc gcatggcagc
    26761 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    26821 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    26881 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    26941 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    27001 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    27061 aatgctcagc tcagccagtg cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    27121 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    27181 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    27241 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    27301 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    27361 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    27421 ttctgcagca gtcatatctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    27481 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    27541 gtcggtcaga ccaaggtctt tacccacttt caggagcagt gcgcccatcc cgtcggcaac
    27601 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    27661 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    27721 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    27781 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    27841 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    27901 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    27961 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    28021 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    28081 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    28141 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    28201 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    28261 acgagaaggc accaggtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    28321 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    28381 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    28441 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    28501 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    28561 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    28621 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    28681 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    28741 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    28801 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    28861 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    28921 tgccgcaccg taagagcctg gcccgcgctg gaacgcctcg ttaccacttt atatcgggcc
    28981 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    29041 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    29101 tcaggagtca gcatg
//
LOCUS       DAJTRB010000047        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTRB010000047 DAJTRB010000000
VERSION     DAJTRB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.047"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO330_002200"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO330_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5883054.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO330_002201"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO330_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5883055.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO330_002202"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO330_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5883056.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO330_002203"
     CDS             4843..5082
                     /locus_tag="OO330_002203"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883057.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO330_002204"
     CDS             5092..5262
                     /locus_tag="OO330_002204"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5883058.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO330_002205"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO330_002205"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5883059.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO330_002206"
     CDS             6724..6990
                     /locus_tag="OO330_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5883060.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO330_002207"
     CDS             7084..7773
                     /locus_tag="OO330_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5883061.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO330_002208"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO330_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5883062.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO330_002209"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO330_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5883063.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO330_002210"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO330_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5883064.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO330_002211"
     CDS             complement(10475..12193)
                     /locus_tag="OO330_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5883065.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO330_002212"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO330_002212"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5883066.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO330_002213"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO330_002213"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5883067.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO330_002214"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO330_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5883068.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO330_002215"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO330_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5883069.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO330_002216"
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO330_002216"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR5883070.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18432..18746
                     /locus_tag="OO330_002217"
     CDS             18432..18746
                     /locus_tag="OO330_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5883071.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO330_002218"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO330_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5883072.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO330_002219"
     CDS             20570..21862
                     /locus_tag="OO330_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5883073.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO330_002220"
     CDS             complement(22016..23665)
                     /locus_tag="OO330_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5883074.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO330_002221"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO330_002221"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5883075.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO330_002222"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO330_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5883076.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO330_002223"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO330_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5883077.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO330_002224"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO330_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO330_002225"
     CDS             28753..29034
                     /locus_tag="OO330_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5883078.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7285 a         7776 c         7351 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ttcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggatcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttggcc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttattat gggaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccaacaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaaaaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcaccag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcgac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagaccta
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcgtcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTRB010000048        28928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTRB010000048 DAJTRB010000000
VERSION     DAJTRB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.048"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO330_002226"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO330_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5883079.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO330_002227"
     CDS             complement(2138..2584)
                     /locus_tag="OO330_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5883080.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO330_002228"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO330_002228"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5883081.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO330_002229"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO330_002229"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5883082.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO330_002230"
     CDS             complement(5347..6009)
                     /locus_tag="OO330_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5883083.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO330_002231"
     CDS             6182..6595
                     /locus_tag="OO330_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5883084.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO330_002232"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO330_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5883085.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO330_002233"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO330_002233"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5883086.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO330_002234"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO330_002234"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5883087.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO330_002235"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO330_002235"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5883088.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO330_002236"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO330_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5883089.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO330_002237"
     tRNA            complement(9861..9948)
                     /locus_tag="OO330_002237"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO330_002238"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO330_002238"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5883090.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLIALDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQSTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO330_002239"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO330_002239"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5883091.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO330_002240"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO330_002240"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5883092.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO330_002241"
     CDS             14005..14592
                     /locus_tag="OO330_002241"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5883093.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO330_002242"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO330_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5883094.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO330_002243"
     CDS             14984..15841
                     /locus_tag="OO330_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5883095.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO330_002244"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO330_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5883096.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMVLWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO330_002245"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO330_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5883097.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO330_002246"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO330_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5883098.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO330_002247"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO330_002247"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5883099.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OO330_002248"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OO330_002248"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5883100.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OO330_002249"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OO330_002249"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5883101.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="OO330_002250"
     CDS             complement(22898..24037)
                     /locus_tag="OO330_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5883102.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..26179)
                     /locus_tag="OO330_002251"
     CDS             complement(24083..26179)
                     /locus_tag="OO330_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5883103.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QPYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26179..26925)
                     /locus_tag="OO330_002252"
     CDS             complement(26179..26925)
                     /locus_tag="OO330_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5883104.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQF
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26922..27566)
                     /locus_tag="OO330_002253"
     CDS             complement(26922..27566)
                     /locus_tag="OO330_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5883105.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27673..27978)
                     /locus_tag="OO330_002254"
     CDS             complement(27673..27978)
                     /locus_tag="OO330_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883106.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28420..28632)
                     /gene="cspH"
                     /locus_tag="OO330_002255"
     CDS             complement(28420..28632)
                     /gene="cspH"
                     /locus_tag="OO330_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5883107.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7007 a         7329 c         7605 g         6987 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgattgc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaatctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg tgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtctagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtaagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt cagggcggta agttggtttg
    25261 ctaccgtggt gtactgcaaa ccttcagact ctggcgcagg ctgccaggcc ggttttggcg
    25321 tatcgcgtag ggcagggcgc agatcgttga aattggtccg taaagtgaaa ccaaacatca
    25381 acgtgtttcc gcgttcataa ctgaggttga ggtctgccca gctggcagcg cgataaactg
    25441 caccgacgtt gaaatggctt gcctgaggca gtttgccagc gaaatcattc tggtaattgt
    25501 tgccgtcgta ttcgagtttc agacgcaggg gattccacgg cgtttgatac tcaatgccgc
    25561 caaagatgga agccgggcca cgaaagatat cgctaaagct gatatcgccc gcatcgtgag
    25621 actctgcgcg atgacaatat ttatcgctca cccggcaaaa cgggttggta atattgcccg
    25681 cattaccggc gtagccccat gccatcccga gggtgaaatc aaatggcccc gcttgcttgc
    25741 tggccaccag atattcaccg tcaaacaaac ccgtaccagc aatatcacgt ttaccaaacg
    25801 ccacttgcgg tagccagtaa ccttcttccc acaggcgaag tttaaaatcg aatgatttgt
    25861 ctttgtatgg ctgatcgccg ctgaaatctt cccactggct atatttgcgt gtgcgcacat
    25921 ccgtataacg aatggtgcct tccagccatg ggaacagcgc cacggaggtg gagtagaacc
    25981 ggtattgatc gttatcccgg tagttgacgc tgaattcacc ttccggtgcg atgcgcgcat
    26041 ttggcatctg caacaatcct gtgccgccaa agtctgattg cgacgaaccc agcggatccg
    26101 ggtaggttaa tacttcagca tgacaggcac tggagacggc aatggccagg cagcttaaaa
    26161 gataagaatt cttcttcatc agtcaggaac acgttgcgtt aaaacagaca caatctggtc
    26221 atttaaatca gcatattttt cgggcagtac atgcgcagaa aagcccaacc aaaactggct
    26281 gccaggcggt ggttccacat gtcttttatt ccacagggca acaggggcga cgacagtctc
    26341 accctcaggc gtaatcacca taacgttatt cttgtctgcg cctgcaaggc gggggtgatc
    26401 ttgcagataa tcggtaacgc tacggcctgc ctgccagggc aattgcccag ccccagaaac
    26461 cgcacccagc agagtaatag tcaccggacg ctgtacggta tagagcgtat agtccccgac
    26521 caacggggga ttgctgttct catccactcg tacaaaatca gggtccagtt tgaccggtaa
    26581 gcgaccagtg atattgagat tgagtaactg ctggcgcacg gatttaatcg ttgccgccac
    26641 atcgtcgtca gcttctgctt cccaggaagc aagctgtgcc atgacatgtt ggtagtcttt
    26701 aagcgcttta gctttcgccg cagagtcggt cagcaaggca ccaggccacc agaggcgatc
    26761 gcgcagttgc ggttgtgtca ccaactggac aacattttcc acggggccaa cggaaagtgt
    26821 ctgttgttcg ctaggcagat aaatagtcac cgttccttgc gcaaaggcat ggggtgtcat
    26881 tacgtaaagt acgctggcaa tgaaatacga ctgtaattta ttcatggtgc gggtttaaga
    26941 aacgtcattt ctacgggaat aacccctgcg cctaacattt gccgactttg acgaacctgg
    27001 ccagtagccg aatcgaccca gaatgtgttt tgccactgtt tttctggcag cgtggaagtc
    27061 acctcttcct gccagacatt acacagcaca ttgtgacccg caatattcag cgtctctaat
    27121 ccagcaaagg aaaaagtaga acttagggtc gcagagcgaa aatggttgtc ttcactccag
    27181 agaatatcgc gcgtccagcg gctgccatct tttattgcca gcgcgttacg cagaggggcc
    27241 cgcccgggat ttgtcacttc cagcagatta ttattaagtt tgacggtctt taaaagacgt
    27301 ccattgtggg taaccagcat ggcgttatcc tgggataacc atttgctttg ttcttgttct
    27361 atatatccca gtaccacaaa gattcgttgc ccttcattaa ggcgtaaata catcgtggaa
    27421 taaggcaacg cctggatctg ctgagccgct acagtgatat cctgattatc gaaaaggctg
    27481 gcgcgaaatg tatcgaccat actttgctgg ctgtgcgtac atcccgcaag aaatagtgcg
    27541 aaaatcgata aaataagagg gcgcaccttt aatccttgaa aggttaatta aaaaaatgca
    27601 aatctctaaa gcccggtacg aatatcgcaa ccgggtatta tttaacttga atttcgacgc
    27661 gtttaagcag aattagcgcg tagtggacgt ggtggttgag gtgttatgag aaccgccatc
    27721 gccactgtca tttgctgcaa ctactgccaa cgtcgccaca ccaacagccg ttgccacacc
    27781 gacaactgtc gccgtgccga cagcgccagc agccacgcca ctagtggtag ccgcagtcgt
    27841 ggttgcacct gtagtggtcg ttgtggtgcc agtagtaccc gtcgttgcct cggtcccgtt
    27901 tgctgcttca ggggcagcaa acgcagctgg cgtcgtcaac atgaaggcca taaggattgc
    27961 agaaagcttg tgtttcataa cttttccttt attcatcgca tggacaatac gtacgcttga
    28021 gatcaggatt gtctactttg agtataacat ccttgtgaaa tctccctttc ctcataccgc
    28081 tagcactata tactgcgggt gggaaaggcg cgcagaggga aatataagat tgttaacgat
    28141 ttccctctgg aaatacgact tgtgagcata agataaatct tagaaaaata cgaattaaag
    28201 ataagaaaat tgttacgaaa gctattaatt attgttggta atatagtttc aagtggtact
    28261 ataactggct gttaaatggc atttataact attaggtgca gagatattcg cttaaatgga
    28321 ttagtttctt agtggaaaag taactgatat tataatgtat ggtgttgact attgaactat
    28381 tccaataaaa ccagtcagct ttaaacaagc agcgtcatat taagagagat aaacatttgc
    28441 cgctgttggt cctcgcaggc catttacgcg gcaaaattcc acgcgtaatc ctggtataag
    28501 cacttctgcg tcgcggggag tgaatgcgga aatatggacc tggacttctt tacgaccgtc
    28561 ggaggggata atgaatcctt tgccgctttt gcgatcaaag gttttgacaa ttcctgtcat
    28621 tttacgggac aaacaaattc cttactgaaa atactgcgct gcactatacg gggttaataa
    28681 aataaagcca gcgatattta agaccgccgg acggctaaaa taaaatttgc ttaatctcaa
    28741 ttatcatgcc ttaatagctg cgtcggtttg aaagacagac agcatacaaa gtagtttact
    28801 aaagcagttc tcattatcag gcattatccc cttcttttga gtctctctcc tgaacactaa
    28861 gtggtttctg tattaaagcc ctgtttgccg aaaggcccaa aatgaaggaa gtaaaatatg
    28921 tctaataa
//
LOCUS       DAJTRB010000049        28822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTRB010000049 DAJTRB010000000
VERSION     DAJTRB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.049"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..215)
                     /locus_tag="OO330_002256"
     CDS             complement(<1..215)
                     /locus_tag="OO330_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5883108.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYR"
     gene            complement(465..1871)
                     /gene="gndA"
                     /locus_tag="OO330_002257"
     CDS             complement(465..1871)
                     /gene="gndA"
                     /locus_tag="OO330_002257"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5883109.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1980..2339)
                     /locus_tag="OO330_002258"
     CDS             complement(1980..2339)
                     /locus_tag="OO330_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883110.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRIYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(2336..2842)
                     /locus_tag="OO330_002259"
     CDS             complement(2336..2842)
                     /locus_tag="OO330_002259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883111.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(2988..3551)
                     /locus_tag="OO330_002260"
     CDS             complement(2988..3551)
                     /locus_tag="OO330_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883112.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(3758..4696)
                     /locus_tag="OO330_002261"
                     /pseudo
     CDS             complement(3758..4696)
                     /locus_tag="OO330_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(4759..5133)
                     /locus_tag="OO330_002262"
     CDS             complement(4759..5133)
                     /locus_tag="OO330_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5883113.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(5137..6285)
                     /gene="wzy"
                     /locus_tag="OO330_002263"
     CDS             complement(5137..6285)
                     /gene="wzy"
                     /locus_tag="OO330_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5883114.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(6305..7210)
                     /gene="rfbG"
                     /locus_tag="OO330_002264"
     CDS             complement(6305..7210)
                     /gene="rfbG"
                     /locus_tag="OO330_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5883115.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(7203..8093)
                     /locus_tag="OO330_002265"
     CDS             complement(7203..8093)
                     /locus_tag="OO330_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5883116.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(8123..9379)
                     /gene="wzx"
                     /locus_tag="OO330_002266"
     CDS             complement(8123..9379)
                     /gene="wzx"
                     /locus_tag="OO330_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5883117.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(9395..9940)
                     /gene="rfbC"
                     /locus_tag="OO330_002267"
     CDS             complement(9395..9940)
                     /gene="rfbC"
                     /locus_tag="OO330_002267"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5883118.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(9945..10823)
                     /gene="rfbA"
                     /locus_tag="OO330_002268"
     CDS             complement(9945..10823)
                     /gene="rfbA"
                     /locus_tag="OO330_002268"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5883119.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(10882..11781)
                     /gene="rfbD"
                     /locus_tag="OO330_002269"
     CDS             complement(10882..11781)
                     /gene="rfbD"
                     /locus_tag="OO330_002269"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5883120.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(11781..12866)
                     /gene="rfbB"
                     /locus_tag="OO330_002270"
     CDS             complement(11781..12866)
                     /gene="rfbB"
                     /locus_tag="OO330_002270"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5883121.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(13239..14132)
                     /gene="galF"
                     /locus_tag="OO330_002271"
     CDS             complement(13239..14132)
                     /gene="galF"
                     /locus_tag="OO330_002271"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5883122.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(14307..15701)
                     /gene="wcaM"
                     /locus_tag="OO330_002272"
     CDS             complement(14307..15701)
                     /gene="wcaM"
                     /locus_tag="OO330_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5883123.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(15712..16932)
                     /gene="wcaL"
                     /locus_tag="OO330_002273"
     CDS             complement(15712..16932)
                     /gene="wcaL"
                     /locus_tag="OO330_002273"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5883124.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(16929..18209)
                     /gene="wcaK"
                     /locus_tag="OO330_002274"
     CDS             complement(16929..18209)
                     /gene="wcaK"
                     /locus_tag="OO330_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5883125.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(18389..19867)
                     /gene="wzxC"
                     /locus_tag="OO330_002275"
     CDS             complement(18389..19867)
                     /gene="wzxC"
                     /locus_tag="OO330_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5883126.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(19869..21263)
                     /gene="wcaJ"
                     /locus_tag="OO330_002276"
     CDS             complement(19869..21263)
                     /gene="wcaJ"
                     /locus_tag="OO330_002276"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5883127.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(21318..22688)
                     /gene="cpsG"
                     /locus_tag="OO330_002277"
                     /gene_synonym="manB"
     CDS             complement(21318..22688)
                     /gene="cpsG"
                     /locus_tag="OO330_002277"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5883128.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(22881..24317)
                     /gene="cpsB"
                     /locus_tag="OO330_002278"
     CDS             complement(22881..24317)
                     /gene="cpsB"
                     /locus_tag="OO330_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5883129.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(24320..25543)
                     /gene="wcaI"
                     /locus_tag="OO330_002279"
     CDS             complement(24320..25543)
                     /gene="wcaI"
                     /locus_tag="OO330_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5883130.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLVTAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(25540..26019)
                     /gene="gmm"
                     /locus_tag="OO330_002280"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(25540..26019)
                     /gene="gmm"
                     /locus_tag="OO330_002280"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5883131.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(26019..26987)
                     /gene="fcl"
                     /locus_tag="OO330_002281"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(26019..26987)
                     /gene="fcl"
                     /locus_tag="OO330_002281"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(26990..28111)
                     /gene="gmd"
                     /locus_tag="OO330_002282"
     CDS             complement(26990..28111)
                     /gene="gmd"
                     /locus_tag="OO330_002282"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5883132.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(28137..28685)
                     /gene="wcaF"
                     /locus_tag="OO330_002283"
     CDS             complement(28137..28685)
                     /gene="wcaF"
                     /locus_tag="OO330_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5883133.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(28701..>28822)
                     /locus_tag="OO330_002284"
     CDS             complement(28701..>28822)
                     /locus_tag="OO330_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5883134.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         7896 a         7131 c         6138 g         7657 t
ORIGIN      
        1 cggtaggctt catttttatc taatgtggca ttaaagtgta ttttatctga ttgcaaaaac
       61 tgctgaattt ccttatcaac aataggagat atccgatcat tcagcatagc aacgcgtgac
      121 ggtaaaatat ctaatgccac aacctcatga ttttgtgcga ttagaagccc gtttgacaag
      181 cctacatagc cagtaccgga aatggtgatt ttcatttcat gctctcagaa ttaacttaac
      241 tgtgaatcat gatgttttta gcatcgtgat aagatgctaa aagttttaac gctacgttgt
      301 cagatggtca acgcaacgaa aaacaattca gattttcttt ataagaatag ctgatgttta
      361 ttaagttaat attaagcagt gaaatttagt ctataagata tttggcaaaa aaaagcccgg
      421 tgcaatatac gccgggcctc aattttattg ttggttaaat cagattaatc cagccattca
      481 gtatggaaca caccttcttt atcaatgcgc ttataagtat gcgccccgaa atagtcacgc
      541 tgtgcctgga tcaggttcgc aggcagaacc gcggcacggt agctatcgta ataggcaacc
      601 gcagcggcga aggtcggaac cgggataccg ttctgtactg cataagcgac gacatcgcgc
      661 agcgcctgct ggtagtcatc ggcaatttgc ttgaagtacg gggccagcag caggttagcg
      721 atctgcggat tttcggcata agcatcggtg attttctgca ggaactgcgc acggatgatg
      781 cagccagcac ggaaaatctt cgcgatttcg ccgtagttca gatcccaatt gtattcttca
      841 gacgcagcac gcagctgaga gaagccctga gcgtaagaaa cgattttgcc cagatacagc
      901 gcactgcgaa ctttttcgat gaactcagcc ttgtcgcctg ctgactgagc ttgcggacca
      961 gagagaactt tagatgcggc aacgcgctga tctttcagag aagagatata acgtgcaaac
     1021 acagactcgg taatcagcga cagcggttcg ccgagatcca gcgcgctctg gctggtccat
     1081 ttaccggtgc ctttgtttgc tgcttcatcc agaatcacat caaccaggta gttaccgtct
     1141 tcatcttttt tggtgaagat atctttagtg atgtcgatca ggtagctgct aagttcaccg
     1201 ttattccact cggtgaaagt ctgggccagt tcttcattag atagattcag gccacctttg
     1261 agcagagaat acgcttcagc aatgagctgc atatctccgt attcaatacc attgtgaacc
     1321 atcttcacat aatggcctgc gccgtcggca ccaatataag taacgcatgg ctcgccgtct
     1381 tctgccacgg cagcgatttt agtcaggata ggcgcaacca gctcataagc ctctttctgc
     1441 ccgccaggca taatagaagg acctttcagc gcgccctctt caccgccgga aacaccggta
     1501 ccgatgaagt taaagccctc tgctgaaagc tcacgattac gacgaatggt gtcctggaag
     1561 aaggtattac caccatcaat gatgatgtca cctttatcga ggtatggttt cagggaatca
     1621 atcgcagcat ccgtgcctgc acctgctttc accattaaca ggatgcgacg aggcgtttcc
     1681 agagattcaa caaactcttt caccgtatag taaggagcca gtttcttgcc tggattttca
     1741 gcaatcactt cttccgtctt ttcgcgggag cggttgaaaa tagagacggt ataaccacga
     1801 ctttcgatgt tgagcgcaag gttgcgcccc atcactgcca taccgactac gccgatctgt
     1861 tgctttgaca ttgtttactc ctgtcttcta tattattaac cgtctattaa ccgttatatt
     1921 attaaccgtc gtgattttca tcttctaatc aaggaataca tatggttaca gcatgcttct
     1981 tactttatag cgataagtat gccattgggt gtatccataa gaactttgaa ttttggatac
     2041 ttttctggat taaaattagc atagattcgt ttagatataa caataaacga actattaaaa
     2101 gatttcatct tctctattaa atcatcctgt gggattacct cacactcggg attactaata
     2161 gtccaatcac tatatcttgc taatccaaag acgtcaccaa ttgccatccc tttatagaag
     2221 taaataccat cctccaagcc taattggata agattatctc catataactc actatattca
     2281 cttgctttct tcatcagttc atagctatag cgactttcta agcgtgaatt aaaattcaag
     2341 tattcagaat aaaaatattt gaattcaaaa tacaacgatc gccccgaaaa aaaataatac
     2401 acacaaggat aaaatatcgt cccaacttgt aatttgtaat gttgatatct tttattaaac
     2461 ttgcaattga gatcgcaacc tgcaatactg acaatgggta aatgggtgat aaatacctgt
     2521 caccttgcgt aacagaaaac caaaataaga catatgttaa aacaatcgag aaaaataccc
     2581 aaagtatttt attattccta agtgtaaaga cagacagtat agcataaaaa acaacatcac
     2641 tgccaacttt attaattgct tgttgcaaat ctaatgtggc aggacttata ccatgtcgag
     2701 cttgttctgc tgtttgaact tgtagatcaa tttcattcca taggtagtga ccaaatatat
     2761 ttcctccagc aggactaaaa ggatcaccag agatccaaaa actacggaca taccaataaa
     2821 tgccaccaag aattgttgcc aagctaaaaa taagaaatgt ctgtatgttt ctctttttcg
     2881 ctatcaatat aacaaatatg atggctccat atatggcacc aaatagtttt acgccacaag
     2941 caattccaat aaaaaaacca cataaaaaaa ttaattcatt ttgtgtttta ttgttttctg
     3001 agcgaatata tataagaata ctggctgcag caaaacaaag atagcgttaa atcaatatat
     3061 gcataaccaa gatgatcttt aagtggattg atagtaaata gtaggattgt agcaataatg
     3121 cctggataat aagttttcgt aatttcccat gaacagccaa caagtccaca ggctattaca
     3181 aaaaaacaga tatttgctaa aaactgagct aaccttacat ctcctaactg taaacctaaa
     3241 gccattaaaa gatctgcatt ttgcgggaac agaggaaaac gcaaatattg ttcaacaaca
     3301 attgcatggt gctcaactaa tgaacgagcc aaaggtaagt gatacatagt atcatcccaa
     3361 tcaccgggag ctcgcaggga cgataacaat gtaaccacaa aaatcaaaac aagaaataaa
     3421 ataacaaaca gcgtactgaa ctcaatatta tttattgcca gccatctaaa ttgctgacgt
     3481 ttaataatgc ttaaaaccca acacaccgta aaaattaaaa agatagcaat cttattaaat
     3541 tgcccgacca tgcccagaaa gtaaattatc aaaatattta aagacatccc tatgcatagg
     3601 caataaaaaa taaagaaaat ttgttaagtc ctgccacccc ctccttaaaa aaagatgaca
     3661 atacaatatt accaatgaca aaaaatgtca ttccaaaagc aaaacaatac acaacataca
     3721 aatacaacgg caattcaatc atagtaaaat actctattta cattatcagt ttatagtttt
     3781 tttcagaaag ataaaataat gccttcattt cacgtctagt tgttgtattt gaatgcaaaa
     3841 taatgccaca tacaaatgcc attattgacg ataacatgat ggaggcagtt aaaaaggcag
     3901 tcggaaatct tctcaccaaa cctgtatcaa tataatcaac aatgatgggc aatcctaaaa
     3961 ttatagacaa agcagcaagt aaaacgccga taatactaaa aaagtatagt ggccgttcaa
     4021 gcgagtaaag tttaattatt gttttcagaa tcttgaaccc atcactccaa gttgatagtt
     4081 tgctcacact cccttcagat cgctcgccat attttgtatt aacctcacca tacttcatac
     4141 gtaattctaa agcatgaatt gtaagctcag tttcgatttc aaatcccctt gatagacatg
     4201 gaaaagattt aacatagcgt ctggaaaata cacgataccc cgacaacata tcagtaaaat
     4261 tccctttaaa gatagtctga acagtaccag tcaacagttt atttcctcag cgatgccctt
     4321 ttctgtatgt attttgatct ccattctctt gacgacatcc tacaaccata tccaattgat
     4381 tttttatgag atggttaatc atttctggag cagaacttgc atcataagtg tcatctccat
     4441 caaccattag ataaatatcc gcatctacat ctgagaacat acgtcttacg acattcccct
     4501 tgcctttcag aggaacagag tgaactgttg ctccagcatc ctctgccact tgtgctgtac
     4561 tgtcagtcga attattatca aatacatata tagaagcagt tggaatattc gttttgaaat
     4621 catttatgac ttttgctatt gccttctgct cattataaca aggaataatt acagcgatat
     4681 taaaagagtg atacataagc ccctcatata aactatttag ctaatgtgca agtaattgtg
     4741 tttttaacaa atcgtttact aacgaaaaat gaaatatttt gatgcgcaat atcctaaaac
     4801 caaactaaaa agagaaaagg tgaacaatgt aaatattgga gataaggcta agagatcaaa
     4861 taaaacgccg aataaataac tcatcgcccc cataaaaaat ataaatataa aatatctagc
     4921 cttggttgca gatactttaa atgagcatct tgcatttgca aaaaaagaaa aggttgctgc
     4981 acatataaaa ccaacaatgt tacttcggcc ttgaccaaaa gctaaattat tataacacgc
     5041 ataaaatacc ccccaatgta aagcagtatt gacaattcca attaaaaaat aactaaaaaa
     5101 agaactggtc aataacttcc ctatttttaa catcctttat tttgctccag aagtgaggtt
     5161 attactaatt tggatatttt ctatagaaaa tacccataaa actacaacgc ctataataaa
     5221 aattcccatg ggtgtaaaaa gaaatggatt agttatagaa acggtaatac tgaaaaaaag
     5281 tattaacgac atcattaatg atgtttttgt actatttaaa aaatagcctt tcaaaaagat
     5341 aagcaacata ggaactaatg atataaatac accaataacg cctgttttct gaagtatctc
     5401 aagaggtact atttcaatcc ttaatcgatc taatataaat gaaccaaatc ctcttccaaa
     5461 caagaacgtc gctaaatcaa catttttata ataaaaatat aaatcattaa atctaacaga
     5521 gtcagaatct tcactgcgtg atccgaccat gtaaagcata attataaata gaacgctgcc
     5581 caatattatt ataatagtat ttaaatatag cttatgtaat ttaaaactgg ctaataacag
     5641 tgataatgtt gtaaaaagta atacacctct tgattctgtc aataatatcg caagcactat
     5701 tatgagacat tttaaatatt ttttgtcgac aaaactgata atcgcaccga caccaaaaaa
     5761 gtagaaggat ttggaaaaaa aagccccatc gggacgaaaa aagaactcgc tagtcaatga
     5821 taagtgttca taaattaaat taaaatttaa caaaccgaaa ttcaacaaaa ttatgtatga
     5881 aatatataac agtgatccat ataatgcaaa tgcattaact attttaataa aaatctcaca
     5941 tgaaaatact tttaaagtat atcttagtgt aaatagatat ataagtgaca gcataaaaag
     6001 gtatggttgc acatcaaaca gtatatcatt tattttatta ccagctaata agccgacaaa
     6061 gaagccataa aaaggaaata aaagcaacca tataaaaaga ctgacacatt tttgggtgat
     6121 tgaaaaattg aatgtaacta aattaaaaat taaaagtata aaactaaata caaataataa
     6181 atatcgcaaa gggaatattc ccaatggctc cagtaatctt ccaccaccac caataatcag
     6241 ttcaatacat aaaaatgtta taaaaatttt atttatatta ttcatattcg taaggtgatg
     6301 ttttttaaaa tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc
     6361 gccttaactt ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt
     6421 gattattcac aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat
     6481 accacttgtt tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc
     6541 ccacttggtt gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg
     6601 aataatttct cgtccgcgca aatgaatata aaaaccaatc atgcagacag atatcttgtg
     6661 cagcattttt attatttatg atgaacttct taaattcaat aagcgtttct ttttttataa
     6721 catatgtaca ccccggacct gctgcttcaa aaaaataatc gaattgagtt tgcgactgag
     6781 ccttatcaac taaatttttt cgccctgatg gataatatgc aattacatta ctggaataac
     6841 aattcgcatt attaaatacc aagcagtcaa ttgcattttt tattttatct ttataccaaa
     6901 tatcgtcctg atcactaaaa cagatataat catagttttc aagatcgaca tctttaatta
     6961 atctataaaa atttttacct gcccccccaa acttattgac gcttggaagt atatttataa
     7021 ttgagggata tgataattga aattcttcac aaatatttaa tgtattatca gttgataaat
     7081 catctgatat aaaaattgag atgtctacat ctttttgttc aataatactt tttaattgtt
     7141 cctcaatcca gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag
     7201 aactatgcat tatagcgacc gcccccagta attcctctta ttccatccag cgtagccaaa
     7261 atatgttttt tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga
     7321 attaaagaaa aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc
     7381 aaaagaaatg catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta
     7441 atcccacaga tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg
     7501 atatgaacta atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca
     7561 ataaaaagtt cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatagtt
     7621 ccggatgaga ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc
     7681 tctcgaagac catttttttt gatattacaa ataggataat aatgtgactt atctctatca
     7741 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
     7801 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
     7861 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctaccaaga
     7921 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
     7981 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag tgcattcaga
     8041 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
     8101 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
     8161 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
     8221 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
     8281 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
     8341 ccagcgcatt gaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
     8401 gataattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
     8461 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
     8521 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
     8581 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
     8641 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
     8701 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
     8761 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
     8821 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
     8881 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
     8941 tttcatcccc ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa
     9001 tagccaacag tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat
     9061 aattaacaat tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca
     9121 ccaaaaaaga tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa
     9181 ttgcaatttc tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa
     9241 caagtgcaat tgcaattgta taaataccga aaccttcagg ccccaaacct cgagcgagaa
     9301 atcctagcga ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac
     9361 tattttttat tatgctcatt attacatttc tttatcatat aaaataatca gcatctacaa
     9421 atttttgacc attaatatct ttatcagaaa gcaatggatt ttgaaccggc cattctatat
     9481 ttatactttt gtcgctccat aaaatcccac gctcttgcat ttttgaatag aaattatttg
     9541 ttttgtagac aaattctgct tcatcactaa gaacgcaaaa cccatgagcg aatccttccg
     9601 gtatccataa ttgacgctta ttctccgccg acaaatttac accaacccac ttgccaaatg
     9661 ttggtgatga tttccgaata tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta
     9721 ccagttttcc ttgagcataa ggttccagct gatagtgcag tccgcgtaac acacccttag
     9781 ttgatttaga atggttatcc tgaacaaact caacctttcg tcctacagcc tcttcgaaaa
     9841 ctttctgatt gaagctttcc ataaaaaagc cccgctcatc accaaatact tttggttcaa
     9901 aaattagcac atcaggaatt tcagttttaa ttacgttcat tttattaata acctttaatc
     9961 attttcagca gatactgacc ataagcattt ttcttcagcg gctcggctaa taccttcact
    10021 tgctcagcat caataaagcc tttacggtaa gcaatttctt ccgggcagga aactttcagc
    10081 ccctgacgct cttcaattgt tgcaatgaag ttgcttgctt caataagact ttgatgtgtc
    10141 cccgtgtcca gccatgcata accacgcccc atcatagcga cagacaaacg tccttggtcc
    10201 atataaatac ggttaatatc ggtaatttcc agttcgccac gtgcagatgg tttaaggttt
    10261 ttcgccattt ctacaacgtc attgtcatag aaataaagcc cagtaaccgc atagttactt
    10321 tttggttcca gcggtttttc ttccagacta attgccgtac cgttatcatc aaattccacc
    10381 acaccgtagc gctcaggatc atttacgtga taggcaaaaa ccgttgcgcc actctctctg
    10441 ttaacagcgg tatccattaa tttcggcagg tcgtgaccat agaagatatt atcaccaagt
    10501 accagcgcac aatcatcacc acctataaat tcttcaccaa tgataaatgc ttgcgccaga
    10561 ccatccggac tcggttgtac tttatattgc agattaagcc cccactggct tccatcacca
    10621 agcaattgtt gaaaacgtgg tgtatcctgc ggcgtactga taatcagaat atctcgaata
    10681 cccgccaaca taagcgttga aagcggataa taaatcatcg gtttatcata aatcggtagc
    10741 agttgtttgc tcacagccat tgtcacagga taaagacgag tgccggaacc accagccaga
    10801 ataatacctt tacgcgtttt catttcacca ttccttttaa ttcatctcgc tctagcatca
    10861 tgagcgagat gcaataattt gttaaattgc cgtggtcgta aataattcgt tgagcatacg
    10921 tttcacgccc acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttcggt
    10981 attgaggcga gaattatggg gacgacgtgc tggtgtggga taggccgttg tcggcacggc
    11041 attaagtttg ttaagagcaa ggttgatccc cgctctgcgc gcttcttcaa ataccagagc
    11101 ggcataatcg tgccaggttg ttgtaccacc agcaaccaga tggtacaaac cagcaacttc
    11161 tggtttattt gcggccacgc gaatggcatg agcggtgcaa tcagccagta attcagcccc
    11221 cgttggtgcg ccgaattgat cgtttattac agccaactct tcgcgttctt ttgccaggcg
    11281 caacatcgtt ttggcaaagt tatttccttt accagcgtat acccagctgg tacggaaaat
    11341 aagatgcttt gcgcaatgtt cttgtaacgc tttttcccca gctagcttgg tttcaccgta
    11401 gacatttagc ggtgctgttg catccgtttc cagccatggc gtgtcaccgt tgcctgggaa
    11461 aacataatca gtggagtagt gtataaccca ggctccgact tcattagctg cttttgcaat
    11521 cgcttcgacg catgtcgcgt taagtaattg cgcgaaattc ggttctgatt ccgctttgtc
    11581 tactgcagtg tgagcagcag cattaacaat aacatcgggg cgaatttttt tgacggtttc
    11641 agccacacct tcggggttgc tgaaatctcc acaataatca gtggagtgaa catcgagcgc
    11701 aatcaagtta cccagaggag ccagagcacg ctgcagttcc caacccacct gccctgtttt
    11761 gccgaaaagc aggatattca ttactgacgg ccctcatagt tttgttcaat ccacgactga
    11821 taagcaccgc ttttcacatt ctcaacccaa tttgtattag ccagatacca ttccaccgtt
    11881 ttacgaatcc cgctctcaaa cgtttcttgc ggtttccagc ccaattcgcg gctaatttta
    11941 tcggcatcaa tggcgtaacg gcggtcatgc cccgggcgat cggcaacata agtaatttgc
    12001 tcacgataag atttctcttt cggtacaatc tcatccaaca aatcacaaat agtgagcact
    12061 acgtcgatgt ttttcttttc gttatgtcca ccaatgttat aagtttcacc cgctttacct
    12121 tcggttacga cggtatataa cgcacgagca tgatcctcta catacaacca gtcgcgaatt
    12181 tgattccctt taccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat
    12241 ggaataagtt tttccgggaa gtgataagga ccataattgt tcgagcaatt agtcacaatg
    12301 gttggtaaac cataagtccg cttccaggca cgtaccaaat gatcgctgga agctttagac
    12361 gcagaatacg ggctacttgg cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg
    12421 ttattatttg cttcatccgg atggggtaag tcaccataaa cttcatcagt agaaatatga
    12481 tgaaagcgga aacttttttt cttttcatca ttcagagcag accagtaatt gcgcgccgct
    12541 tctaaaagaa cataagtccc cacaatattg gtttcaataa atgccgcagg gcccgttatt
    12601 gaacggtcaa cgtggctctc tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg
    12661 aaaatacgcg acatggctac agcatcgcag atatctgcat gctcaaaagc ataacgttca
    12721 gaatcagaaa catcagcgag tgattccagg ttcccggcgt atgttaattt atcgacatta
    12781 acaacactat cttgcgtatt atttattatg tgacgaacaa cagcagaacc aataaaccct
    12841 gcgccaccag taacaagtat cttcactttt ttattccata tagctataga gcatgctgtg
    12901 aaatagactg ctccagattt gattaataga ggtattaata cacgctaccg cccctggctt
    12961 aacagctacc agtgcactgc atgcatctca acgatgtggc gagcgttagc cactcgcgcc
    13021 aaatccgaaa aattcaaacg ttaattgtct taccaaacag ccgtagaaac aaggaaaatc
    13081 ctggaaaaat ttgaataata tgagctatgt aacttcttgt ttttataggc tttataaaaa
    13141 cggcaacggc aggaaaattc aatgagcaaa acttatgcca cgaatcttca ctgccgttat
    13201 aattttctta tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca
    13261 atacctttac ggaacttcgc cccttctttc aggttgcgta ggccatactt cacaaacgcc
    13321 tgcatatagc ccattttttt gccgcagtcg taactgtcgc cggtcatcag cattgcatca
    13381 acggattgtt ttttcgccag ctcggcaata gcatcagtca gctgaatacg tccccatgca
    13441 ccaggctgag tacgttccag ttccggccaa atatcggcag aaagcacata gcgacctacg
    13501 gccatgatgt ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaatg
    13561 cggctgactt taccctcacg gtccagcggc tctttagtct ggatgacgga gtattcagag
    13621 aggtcacccg gcatacgttt tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca
    13681 atcatggcag caaggttgta acgtagcggg tcggcgctgg catcgtcgat cacaacgtct
    13741 ggcagtacca cgacaaatgg gttgtcacca atggcaggtc gcgcacacaa aatggagtgg
    13801 cctaaaccta aaggttcgcc ctgacgcacg ttcataatga tcacgcccgg cggacagatg
    13861 gactgtactt ccgccagcag ttgacgcttc acgcgctgct caaggagtga ttctaactca
    13921 taagaggtgt cgaagtggtt ttcgaccgcg ttcttggacg cgtgagttac caggaggatt
    13981 tctttgatcc ctgcagccac aatctcgtca acaatgtact gaatcattgg cttgtcgacg
    14041 attggtagca tctctttggg tatcgcctta gtggcaggca acatatgcat cccgagaccc
    14101 gctacaggaa taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct
    14161 gaattatagc ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat
    14221 tacttgtgat gttacgccgc ttcgttgtgg attgcagtag cattgttcct aagtatggct
    14281 ccatttttcc aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc
    14341 ttttccacat taacaatatg gtgattaatc ctgtcgatat cgacggagct ttgccctctt
    14401 tcattcaccg catgaacatt tgcaagagac agcagtgttt cttttttcgc cataaagacg
    14461 ccgcgaacgt ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc
    14521 tgcatcacat tgatatttcg cataaaaaga tgttgcggct tgttgtgtaa ctccagcgag
    14581 gcacgcttca tctcaatgtt agttagcgac acaaaggaga cggcattccc ggcggagatt
    14641 tggatgccgc gcaatttata agcaagatgg gtattatcca gttgaatatt attcactcgg
    14701 aaattttgcg gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg
    14761 gcgctattaa tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgact
    14821 gtcgcgttat caatgcccgc tttcttactg aaatccggcg tgatattgcg ggcattgata
    14881 ttacgaataa caaaatgttt accattttca acatggatca actgccgaca atccgatccc
    14941 gtgatattcg ccacgacaaa gtttttcact gcctggtctt ccgggtagtt gttatcataa
    15001 gtgcttcccg caaggcctat gccgatgccc cagttgattt tgccgttggt acagttgata
    15061 cgctcgatga catggtcaga tatcaaaata tcactgtcgt taattgccac gttccattca
    15121 atggcgtcgc cctgtaagtc gctgaactta cagttggtaa tgttggcacc gataatctgg
    15181 ttatgaaatc cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg
    15241 gtcaggttgc gcatgacccg tttgtttttg ccgccaatat aaatctgcat taccgggcca
    15301 aagccgctca tcgccagccc tttgatggtg cagtcagaac cacgcacatc cagggtaatg
    15361 ttatgcatac cgccgccctc tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc
    15421 cctctgccgt tgccgcgcag gcttccaagg atgtgtaacg ttttgccggg agggatgaag
    15481 atgccggtgt tgatattgtc acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg
    15541 ctgaaggctt gtttaaatga ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg
    15601 ctttgtcggg cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt
    15661 gccgtcagga aggtgcgtcg ggagagtgtt ttaaatggca tacgttctcc tctataaagc
    15721 ctgcagcaag ctggcgagtt ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt
    15781 ttcgcgcgca cgtttgacga ccggagccag ttcgtcggta tccagttggc taaacgccgc
    15841 cagacgttgt gccagtgcgc gagcatcgtt ctcaggcagc agccagccgg atttatcggc
    15901 ctccaccagt tcaggaattc cgctatgcag agtagaaacc accggaatac cgaccgccat
    15961 cgcttccatt agcgccaccg gaatgccttc catatcgcca tccgcgcccg ttaccgatgg
    16021 caacaggaag acatccgcgt cgtcgagcat ctccttcact tcatggctcg gtttaaagcc
    16081 cggcatctct accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc
    16141 ccacaggcca atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg
    16201 gcaggcttca atcgccacat gcaggccttt tttctcggtt aagcgtgcga cagatatgat
    16261 ttccagcggc gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc
    16321 catacgcgat acggcgattt tttctctggg gcagcccatt ttttgcagcc ttccggccca
    16381 cagatcgctt atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt
    16441 gtagtggttg agcacttccc gactggagat atcaataccg tggaagatgg tggcaatttt
    16501 gccgcgaatg atacccagtt cgcgtagttt tgctgcggtt acccccgccg ggccaaaatg
    16561 agcgatgaac acatcggcgc gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat
    16621 caggttccgc gactcggcac catagcgttt gaggttgagc gcatgccagg tatttttacg
    16681 atgaatgcca cgtaaggtct ggctggcgcg gtggcgcagt ttcgctactt tgcccgcagg
    16741 ttcgtcctgt aaccagcggg ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt
    16801 gttctctgta tcgccttttt gcaaagcgac aatctccact tcaaagccca tatcaataaa
    16861 cgcggtaatt tgattgagga cgaaggtttc cgacgacagc gggaatttca ataagaagaa
    16921 gccgaccttc atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattccc
    16981 gtctgacgtt cgcgactgac ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct
    17041 aaggtatccg caaccatcgc ttgcaggctg ccgtccagta aatgacgaat atcaatcgcc
    17101 atctctggca gtcccagctg ctgcataatc ccggcggatt tatgttcata gttgatggca
    17161 atcgccggag tagcaaagtt cattgagata atggcggagt gcaggcgcgt accgacggtg
    17221 agttcacagg ctccgagaat tttgcccatt tccagatcgt tgagttcatc catcactacg
    17281 tggtaacggg caggatcgct gatgtgctgg cgcagattga gcgccaccat gcggtcgtct
    17341 ttgttatagc tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcatcg
    17401 agaatgcgat tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga
    17461 cgtttgtcga acggtgccag ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca
    17521 acgtccagcc agtgttgaac ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc
    17581 agccacgcgg tatcgacgcc atgttccact tttgcggtgg tgatatttct gcgtttcatc
    17641 aaatcaaggc tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc
    17701 gccagttggt taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc
    17761 ggctttttcg ccataaatgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg
    17821 acaaaaaacg atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag
    17881 tcggtgaatc cctgggcgat ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag
    17941 agcaatacct gatgctggta gcggcgacgg aggacttttt taatgcgccc gacaacgccc
    18001 gccgcgctat tgtgttgtgt catctgcagg aacagcggat cgcccattac cgggcggttg
    18061 agcagccagg aagaactgac cggatagcgg ctcatcacgt ccacttcggt atgtggattg
    18121 aggatgttga tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa
    18181 gtgtggttgc ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg
    18241 ctgtgtttgt tgtgttcgtt gccgtttttt gcctgatgcg acgctggcgc gtcttatcag
    18301 gcctacaggt cccgagcaca gaaccgtagg acggataagg cgttttacgc cgcatccggc
    18361 aaccgttgtc ggaaccgaaa acagcaattc accccgcccg taaaagcatt ttcatttttt
    18421 cgctgcgaca aaactgacgc ttcacttcca ccaccagcgg atggcgtgac agcacaatca
    18481 tcaccacaaa cgccagcacg cccgcggcta tttgcaccgc cagcagcatc cccagcgcca
    18541 gttgcccttt cagcacaatg cccagcgcat aactgaccac cagcgtcggc aacgagagat
    18601 aaaacggcag ccacaaactc aggatgtact ggcgataact ggaaccaaga accggtttaa
    18661 tcatcacgaa gtaactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga
    18721 cgccgattgc gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt
    18781 tgaatacgtt gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa
    18841 tcgggttacc cacggagcgc agcagaccca caatacacag caattgcagc accggaataa
    18901 tgctgttcca cttttcgccg aataccagcg gtacgaagtt attcgacacc accatcagcc
    18961 ccagcagcgc cggaaagttg ataatcccca ctatcgacag cagcttgtag aagttaacac
    19021 gcagcttttc ggtgtcgtcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga
    19081 ttgggttcag cttcatcggc ggtacgacgg ccacgttgta cgccaggttg tatccccctg
    19141 ccacgcccgc gccaagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt
    19201 tgatgatgct gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca
    19261 gcgagaagtg cagaccgggg caataaattt tgcgtccaaa gtagccaaac agcagtgttc
    19321 tcaccgcact attgaccaga taaccgagga tcgcggtcat cgccagcggc cagaaatggg
    19381 cgctaactac cgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt
    19441 tgttgaactc cagctccttc tgcatcaacg cgcggaactg ttgcccgtgg gggatcacca
    19501 caaacgccag cgacaatgtt ttaatcaacg gtgccaggtc cgggttattc agcacgtcgc
    19561 cgatgacatc actcaacaaa aacaccgcca cacataccac aagccccagc ccgacgttca
    19621 gccagtacag cgtggtgagt tcaaggtgac tgatttcttt tcgctgaata atcgagttag
    19681 cgataccgaa gtcagaaagc gtatctgcca gcgcgataat caccagcgac acagtcagca
    19741 ggccgaactg gtggttgtcg ataatccgcg ccagcacggt catctgcacc agcccgaggc
    19801 cgatgatgat caccgtggca atagccgacc acttcgcgcc gctgatggtt ttttcacgta
    19861 agctcatatc aatatgccgc tttgttaacg aagcccttga ataccgtcag gaaaacgatt
    19921 ttgatatcga accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt
    19981 tccattttct ccagcgtgtc ggtttcgccg cgccagccat taatctgcgc ccagccggta
    20041 atgcccggtt tcactttatg gcgcagcatg tagccttcaa tgagctggcg atactgttcg
    20101 ttatgcgcta ctgcgtgcgg acgtggaccg acaatcgaca tccccccggt cagcacattg
    20161 ataaactgcg gcaattcgtc cagcgaggtg cggcgcagaa agttccccac tttagtgacg
    20221 cgcggatcgt tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatggaa
    20281 cggaacttcc acactttgat cggcttgcca tccataccat agcgagtctg gcggaaaata
    20341 accggccctg gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc
    20401 agcagcagaa taagcgtcgc cagcacaatg tcttccgcac gttttagcag gcggttaacc
    20461 cccgaaagcg gcgtgtcata cagcggcacc accggaacgc cgttcatctc ttcgagacgt
    20521 gaatggagaa tgttgaaggt aaagacgtcg gggatcagca gcaccgaaca ggtggtatcc
    20581 gccagttgat ggaccagttt tttcactcgt gcgccgtcgc acatttgcat cgcgatatag
    20641 acgttatgaa tcttgcccgc ttttgcatcc tcgaccagct gttgcaggtt acccgcccag
    20701 tcgttagaaa cgccgcccgg tttcgggtcg tggtaaacgc ccaccacttc aaaccctaac
    20761 cacggctgat tacggaaact ctccatcagc atttgtccgg cggctaaatc ccccgctacg
    20821 gcgaccatcc gcttgttgta gccatgatta cgcagccagc ccgccccaat gcgaatacat
    20881 gaacggcaaa ccaccagtcc gatgctggtc agcccatacc acgccagcca gattttcagt
    20941 tgcgtgtcga aatcattgtt gaacgccacc agtccggcgc tgaaaatcac gcttaaggtc
    21001 cagttttgta gcaacagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa
    21061 aaatcggtga tgccgcccag catctggaac accaccagcg taatcagcgc caccaacagg
    21121 tgcatgtaga ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg
    21181 atggtgatat ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc
    21241 tcgcgctttt ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg
    21301 taagggaaga tccgacatta ctcgttcagc aacgtcagca gagttcgcgt tcgcgcttcc
    21361 atcagcggca catcaccgcg cgattccaca ttcaggcgca ccaccggttc ggtattggag
    21421 gtgcgcaggt taaagcacca gtcggcaaag gtcatgctga tgccatcgct gcgatccacc
    21481 gccagcgcct cacggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    21541 agtttgctgt tgatctcacc gcttgccgga aacgccgcca tccggtcgcg caccagttcg
    21601 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    21661 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg
    21721 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga cattaccggc
    21781 gtgccgcctg cagcagtcac cacatcaata gtgttccaag agagacgcgg atcatggatg
    21841 atcttcgcgc cgggattttt ttcgaggaac gcttctgcca gcaggccgac aatgtagtag
    21901 ccctcgataa actgcccttt ttcgtcaaac agaaaacagc ggtcaaaatc gccatcaaag
    21961 gcaatgccca tatccgcgcc gtgtttgatg actgcattgc gggtgtcgtc gcgacattcc
    22021 ggcagcagcg ggttaggaat accgttgggg aaattgccgt ccggtgtgtt gtgtactttg
    22081 attaattcca cgggcgcgcc gagggcttta aagcgggctt caatggcgtc caccaccgga
    22141 cccgctgcgc cattcccgga gttgatcacc agcttgagcg gcgtgagatt tttgacattg
    22201 atataaccga acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg
    22261 cgtttggttt catcgacggg aggaaagtcg ttggcttcag ccagacgctg gacgtcgcgc
    22321 agtccggtat caccgctgat cgtgcgagcc ccctcgcgca ccagcttcat gccgttgtaa
    22381 tccatcggat tatggctggc agtaacttca atgccgccat ccacgccgag atggaacgtg
    22441 gcgaaataga tctcttcggt gccggacata ccgatatcca gcacatcgac gcccgcatcc
    22501 tgtaaacctt ttgccagcgc cagttttaag gtttcgctgg tgagacggac atcaccgcct
    22561 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    22621 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    22681 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    22741 atttttgtcg ttatttacac cgcggtttcg cattcattgc ctgatgcgac gctgccgcgt
    22801 cttatcaggc ctacaaaacc gaaccgtagg tcggataagg cgttcacgcc gcatccgaca
    22861 aacagcgcct gatgcgacgt ttacacccgt ccgtagcgat ccgcgaagcg caccacatca
    22921 tcctcttcga gataagagcc ggagcgcact tcaattaaat cgagcggaat tttccccggg
    22981 ttttccaggc agtgcgtcgc ccccagcgga atataaatgg actcgttttc accaagcagt
    23041 ttgatatcac catcaatggt gacttttgcc gttcccgcga ccactaccca gtgttccgcg
    23101 cggtgatggt gcatctgtac cgacaaaccc tcgcccggtt tcacggtgat gcgtttcacc
    23161 tggtagcggt cgcccgcgtc gatagagtca tatttgcccc acggacgata cacttcgcga
    23221 tgcacccgat gctcatggcg accatcggct ttgatctgct cgaccacttt tttcacatcc
    23281 tgtaccgcgt tacggtcggc aatcagcacc gcatctttgg tctgcaccac taccagatct
    23341 ttcaccccga cggtggtgac caggcctgat tcggcataca catagctgtt ttcagttttg
    23401 tgattaatca catcgccgtg gcaaacgttg ccctcggcgg tgtgggcgct gatctcccat
    23461 aatgaagacc aggaaccaac atcgctccag cccgcatcca tcggcaccac aacagcatct
    23521 gccgtaggtt ccatgaccgc gtaatccacc gactcttccg ggcaggcgag aaacgcttct
    23581 tcatccacac gaataaaatc gagatccgga tcgacggcgc tcatcgcttt ttcgcaggca
    23641 tcgaggatat ccgggcgata ttttttcagt tcttcgagat agcgtccggc gcggaacagg
    23701 aacatgccgc tgttccagta atattcgccg cttgctacat aggcctgggc ggtttccaga
    23761 ttcggttttt cgacaaactg cgccacttca aaggccaccg tatcctgctc acccacagac
    23821 acttcaccgc gacgaatata gccataaccg gtttctggta gatccggcac aatgccaaag
    23881 gtcaccagct tgcccgcttc ggcataaggc atggcattac gcacggcggc acggaacgcg
    23941 tcttcatcgg caatcacatg atccgccgcc aataccagca ttaacgggtc gctctccggg
    24001 ctatgacgtt ttgccgccag cgccgccagt gcaatggcag gtgcagtgtt acgccctgcc
    24061 ggttcgagaa taatgttctc ggtgagtttg ttcagttgac gcagctgttc cgcgacaata
    24121 aagcggtgct gctcattgca aatcaccacc gggctttcgc actccacgcc gttcaggcgg
    24181 cagatggtgg tttgcagcat ggtgagatcg cctttcaggc ataaaaactg cttggggtaa
    24241 agtacgcggg aaagcggcca taaacggcta ccggagccac ctgccatcac aactggatag
    24301 agtttcgact gcgccataat tatccccgaa tatcatttat aaattgacgt aacacgttct
    24361 ctttatcgag cgtgcgttcg gcatattcac gtgccaccgt gttatgtttg ggcagcagga
    24421 gcgcctgacg aatccccgcc accagcgcct cgaccgattc cggttctacg caaaccgcaa
    24481 tacccggaaa ggtttcgcaa agctgcccca gttctgtgtg ggcctctgcg gtaatcaccg
    24541 cgttaccgcc taccgccaga atattggtca gtttcgacgg caatacggca tctgccgcgc
    24601 cgcgtttttg caccaccaga tggcaatcgc ccatcttcag cagtgcaggt aaagcgtcat
    24661 acgattgcag cgggaaaaat tgcatgttgc gcagtccacg ctgctgcgcc attttttcca
    24721 gccgcgcttt gccgccgccc tgcccgacaa tggcaaaaat cagcggttca tcgcgcagga
    24781 gatcggcagc ttcaataacg ttttccagcc cctgcttttc accaatattg ccggagtaaa
    24841 gaatgatttt tttgttatcc ggcaggccaa gctggttacg aagggcatca acatcggcgt
    24901 ctgcaacatg ctgaaaacgg gcaatttccg accagttggg gaagaagatg acgttatccg
    24961 ccgccacgcc tttttcgatg gctttattca tcatcgaacg cgaaatcgtg gaaacgttat
    25021 cgacgttatg cagtccgcta cgttcgaacg ccgttaccag ctgtgccact ttgccgcctt
    25081 tgccttttcc ggcaaggccc agccccagca tggcgtccac ttcgtaatcc tgaatatgca
    25141 gcacggtacg cgcaccagag agcttcgcca gcaggcgcat tcccggcgtg caaaacagcg
    25201 ttggcactac gccaataatg cgatccggct tccagcgacg ttgcgccatc agtggaaaga
    25261 aactgctgac ggcaaaactg ccgagatgca acaagcgttt cagggtgctc ggctgttttg
    25321 gcacgtacag cgggcagcgc cacaccgtgg cagccccttc ttctcgtttg tagcgccagg
    25381 cggaatagtt ctcacccacc tgccattgcg ggtagtaagg cggtgcggta atgacccgca
    25441 cctcatgacc ttgtgccgcc aaccattcca ccatctcgcc ggtgtatttg ccgatgccgg
    25501 ttaactccgg cgagtagtta atgccgtaga ccagtatttt cataatccgg gtactccggc
    25561 acgcttctca gcgagaaaat aggctcggct gttagcgtga acatcgttac tggcgagcag
    25621 cgcgtccggc gtcagccagc ggtaatcgtc atgctgctca tccggcagta acagctcttc
    25681 ttccgctact ctgaagcgaa aaccgagcac cacatagtga gtggtgaaat ccgtgccgga
    25741 gaagttatcg tcataaaaat gctgccagac accgtaaaac tggcctgctg ttatcggcag
    25801 acgcagcccc agttccgcca tcgtcagccg ctcaaatgcg gcttccagcg tttcgtcttt
    25861 ctgcacgcgc cctcccggca caaaccagta accctgcgcc gggcggttgg ttcttttgcc
    25921 aagcagaaac tcgccgcgac tgttctcgac aataaagtcg agagagacaa gcggtgtgga
    25981 gcgcactaca gtggcaaagt cttcctgacg taaaaacatc attccccccg aaagcggtct
    26041 tgattctcaa ggaaccactg gtaagtgctg gcaagccccg cttccagtga gatttcgtga
    26101 taccagccaa gctaatgcag gcgcgtcaca tccagcagtt tgcgcggcgt accatccggt
    26161 ttgctggcat caaaaaccac ccgacctttg taacccacca ctttggcgat ggtttgcgcc
    26221 agctcgcgga tagtgcagtc aacgcccgtg ccgacgttaa tgtgcgacag catcggttgg
    26281 gtattctcca gccagacttc atgcgccagc tccatgacat gaatgctcgc cgccgccata
    26341 tcatcgacgt gcaggaattc acgcatcggt gtaccgctgc cccataccac cacgtccggc
    26401 gcattctgtg ccgtcgcctc gtggaagcga cgcagcaatg ctggtatcac atgggagtta
    26461 ctcgggtgga agttgtcatg cggcccgtac aggttggtcg gcatgacagt gcggtaatcg
    26521 cgtccatact ggcggttgta agattcgcac agtttgatcc cggcgatttt ggcaatggcg
    26581 taaggctcgt tggtcggctc cagcgtgccc tgcaataact cgctttctgc catcggctgt
    26641 tttgccagtt tcgggtagat gcaggacgat ccgagaaaca tcagtttgtt cacgtcgttc
    26701 tgatgcgcgg cgtgaatgat gttgctctca atcatcatgt tctggtagat gaaatccgcc
    26761 ggataggtgt tgttggcaac aatgccgccc actttcgccg ccgccagata gacctggtca
    26821 atgcgttcgc tggcaaagaa atcatgcacg gcgcggctgt ccagcaggtt cagttcatcg
    26881 cgggtacgta ataccagctc cacatcaccg cgctgttcga gctgccgctt gatggcagaa
    26941 ccgaccatcc cacaatggcc agcgataaaa atgcgttgtt tactcatgct tatgactcca
    27001 gcgcgatcgc cacgtcgtag ccgtgagatt tcagcagaga gtgttttttc gccgcttcga
    27061 ggtcattagc caccatttca gacaccatct ctctgagggt gatttccggt ttccagccca
    27121 gtttttcgtg cgctttggtc gggtcgccga gcagcgtttc aacttctgcc ggacggaagt
    27181 aacgcggatc aacggcgata atcacatcac ccggtttaac gcccggcgcg tcatgcccgg
    27241 tgacggaaac cacaatgccc ttctcttcaa cacccgtgcc ttcaaagcgc agtttgatgc
    27301 ccagctgtgc tgccgccatt tccacaaact gacgtacgga gtactgaacg ccggtcgcga
    27361 taacgaaatc ttccggctgt tcctgttgca gcatcatcca ctgcattttt acgtagtctt
    27421 tggcgtggcc ccagtcacgc agggaatcca tattgccgag gtacaggcac gactccagcc
    27481 cctgggcgat gttggcgatt gcgcgggtga ttttgcgggt aacgaaggtt tcgccgcggc
    27541 gcggggattc atggttgaag agaattccgt tacaagcgta catgccgtag gattcgcggt
    27601 agttaacggt gatccagtag gcgtacagtt tggcgaccgc atacggagat cgcgggtaga
    27661 acggcgtggt ttctttctgc ggaatttcct gcaccagacc atacagttca gaggtggaag
    27721 cctgatagaa acgagttttc ttttccagac cgaggaagcg gatcgcctcc agcaggcgca
    27781 gcgtacccat cgcgtcgacg tcagcggtat attctggtga ctcaaaagag accgcaacgt
    27841 ggctcattgc gcctaggttg tacacttcat ccggctgtac ttcacgcaaa atgcgcgtca
    27901 ggttagaggt atcactcagg tcgccataat gcagatggaa tttcgggttg caggtgtgcg
    27961 gatcctgata aatgtgatcc acgcgctcgg tgttgaatga cgatgcgcga cgcttaatac
    28021 catgcacctc gtaacctttt tccagcagaa actctgccag gtaagaaccg tcttgtccgg
    28081 ttacaccggt gatgagagcg acttttgaca tgtcttattc ctctgtattt ttgaatttat
    28141 tcagtttcaa cgcgttcgcg tatcaccact gcggggttcc cccggcaaac cacatttgcc
    28201 ggaagcgatt taaaaacact gcttcgtgca cccacgacgg tgccgtcgcc gattgtgacg
    28261 cctggggcaa caaagacatc ggttgccagc cagcatttct cgccaatcac aataggcgtg
    28321 gcgttaatgg tgaaatgttg acttgcatgg tcgtggctac cggtgcataa ataacttttt
    28381 tgcgatatca ccgaatgtgc gccaatggtt atttcaccga gggtatataa attgacgtca
    28441 tcgccaaccc acgcgtaatc acctaaggtt aatttccacg gataggtaat ttttactgac
    28501 ggacgaataa ctacgttttt tcctattttt gctccgaata aacgtaataa aaaagcccgc
    28561 cagcgataca atacttgtgg cgaccaggca aatattgttg cctgtactgc ccaccataat
    28621 tgcactttaa tagcgttgcc gccccggaac cctttcggca ccgagaaacc gcttaaatct
    28681 tgcattggtt ttccttatat tcagactttg ttatataagg ctttagtctt acctgtcgta
    28741 cgttggcgta aatgccagga taattctgcc cagaagccag gtacatgtaa tatctgccgt
    28801 tggacttttt tggcgtcagc ac
//
LOCUS       DAJTRB010000050        28529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTRB010000050 DAJTRB010000000
VERSION     DAJTRB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.050"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            353..1711
                     /locus_tag="OO330_002285"
     CDS             353..1711
                     /locus_tag="OO330_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5883135.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="OO330_002286"
     CDS             complement(1950..3335)
                     /locus_tag="OO330_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5883136.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OO330_002287"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OO330_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5883137.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4109)
                     /locus_tag="OO330_002288"
     CDS             complement(3729..4109)
                     /locus_tag="OO330_002288"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5883138.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="OO330_002289"
     CDS             complement(4464..4898)
                     /locus_tag="OO330_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5883139.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="OO330_002290"
     CDS             complement(4886..5287)
                     /locus_tag="OO330_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883140.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="OO330_002291"
     CDS             complement(5547..6116)
                     /locus_tag="OO330_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5883141.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6861..7004)
                     /locus_tag="OO330_002292"
                     /pseudo
     CDS             complement(6861..7004)
                     /locus_tag="OO330_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(7338..7604)
                     /locus_tag="OO330_002293"
     CDS             complement(7338..7604)
                     /locus_tag="OO330_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883142.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7745..7951
                     /locus_tag="OO330_002294"
     CDS             7745..7951
                     /locus_tag="OO330_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5883143.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8046..8648
                     /locus_tag="OO330_002295"
     CDS             8046..8648
                     /locus_tag="OO330_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883144.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            8819..9016
                     /locus_tag="OO330_002296"
     CDS             8819..9016
                     /locus_tag="OO330_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883145.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            9848..10765
                     /locus_tag="OO330_002297"
     CDS             9848..10765
                     /locus_tag="OO330_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883146.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            10850..11722
                     /locus_tag="OO330_002298"
     CDS             10850..11722
                     /locus_tag="OO330_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5883147.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            12094..14940
                     /gene="ag43"
                     /locus_tag="OO330_002299"
     CDS             12094..14940
                     /gene="ag43"
                     /locus_tag="OO330_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5883148.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            15011..15169
                     /locus_tag="OO330_002300"
     CDS             15011..15169
                     /locus_tag="OO330_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5883149.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            15324..16142
                     /locus_tag="OO330_002301"
     CDS             15324..16142
                     /locus_tag="OO330_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5883150.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            16484..16957
                     /locus_tag="OO330_002302"
     CDS             16484..16957
                     /locus_tag="OO330_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5883151.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            16973..17449
                     /locus_tag="OO330_002303"
     CDS             16973..17449
                     /locus_tag="OO330_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5883152.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            17512..17733
                     /locus_tag="OO330_002304"
     CDS             17512..17733
                     /locus_tag="OO330_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5883153.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            17896..18270
                     /locus_tag="OO330_002305"
     CDS             17896..18270
                     /locus_tag="OO330_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5883154.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            18317..18691
                     /locus_tag="OO330_002306"
     CDS             18317..18691
                     /locus_tag="OO330_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5883155.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            18688..19179
                     /locus_tag="OO330_002307"
     CDS             18688..19179
                     /locus_tag="OO330_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883156.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            19191..19388
                     /locus_tag="OO330_002308"
     CDS             19191..19388
                     /locus_tag="OO330_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883157.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            19473..20033
                     /locus_tag="OO330_002309"
                     /pseudo
     CDS             19473..20033
                     /locus_tag="OO330_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            20105..21109
                     /locus_tag="OO330_002310"
     CDS             20105..21109
                     /locus_tag="OO330_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5883158.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            21391..21672
                     /locus_tag="OO330_002311"
                     /pseudo
     CDS             21391..21672
                     /locus_tag="OO330_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(21822..21909)
                     /locus_tag="OO330_002312"
     tRNA            complement(21822..21909)
                     /locus_tag="OO330_002312"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21873..21875),aa:Ser,seq:gga)
     gene            22144..23082
                     /gene="ghrA"
                     /locus_tag="OO330_002313"
                     /pseudo
     CDS             22144..23082
                     /gene="ghrA"
                     /locus_tag="OO330_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            23137..23874
                     /gene="ycdX"
                     /locus_tag="OO330_002314"
     CDS             23137..23874
                     /gene="ycdX"
                     /locus_tag="OO330_002314"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5883159.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            23898..24452
                     /gene="ycdY"
                     /locus_tag="OO330_002315"
     CDS             23898..24452
                     /gene="ycdY"
                     /locus_tag="OO330_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5883160.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            24554..25045
                     /locus_tag="OO330_002316"
     CDS             24554..25045
                     /locus_tag="OO330_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5883161.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(25178..25884)
                     /locus_tag="OO330_002317"
                     /pseudo
     CDS             complement(25178..25884)
                     /locus_tag="OO330_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(25911..26327)
                     /gene="csgF"
                     /locus_tag="OO330_002318"
     CDS             complement(25911..26327)
                     /gene="csgF"
                     /locus_tag="OO330_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5883162.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(26352..26741)
                     /gene="csgE"
                     /locus_tag="OO330_002319"
     CDS             complement(26352..26741)
                     /gene="csgE"
                     /locus_tag="OO330_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5883163.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(26746..27396)
                     /gene="csgD"
                     /locus_tag="OO330_002320"
     CDS             complement(26746..27396)
                     /gene="csgD"
                     /locus_tag="OO330_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5883164.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQYLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            28148..>28529
                     /gene="csgB"
                     /locus_tag="OO330_002321"
     CDS             28148..>28529
                     /gene="csgB"
                     /locus_tag="OO330_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5883165.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANIT"
BASE COUNT         7064 a         7211 c         7275 g         6979 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcttttt
     2461 ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagc
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
     3661 gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtttcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gccagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggt
     6001 acatccggga gacgcaggtc aacccgcaat gccgtcaggc ggggatgaac attcagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgtcgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gttctgtcga ccccttcggc tactaccgga
     6421 atacagaccg tatcgttacc atactgctgg cgctgattat tgtggtcctg ctgactactg
     6481 tcagcgtcag tgtgtgtata ctgtgtagct ggtgagataa cggttcatgt atcccctgag
     6541 ctgacagtgt accgtgtata ttaagcgtaa ccgacaggtt ctccttcata ccatattaaa
     6601 ttttccaggg attgccgctg ccttatttcg tcccatacag gaaaactcat ctgggtttct
     6661 gacaaatacg cgaaactcac agcccctcaa tatcaaccac acgttgtaag ccggtaatac
     6721 ctgaaaatca gcctattacc gcccacgcct ccggcaccat tcccttttga tttcgaattc
     6781 tttgttgtcg ctataccgct taatgttccc ggtcctccgg tgcagataac accggagtca
     6841 tcgtagcgct gcttcaatat tcaggtgacg tatttccaga cggaagccgg cgcgcgatcg
     6901 taaagtttgt tcattgtcag ttctgccagc cggttgtcaa cggcagagtt aaaggcttcc
     6961 agctccgcaa gggtaagttt ataacgcgtg tgggaattca cttttcccgc gtctcctgac
     7021 gtattacggg ctgccactta cctggtgtcc cgcaatacag ccttagtgtt ccgttatcgg
     7081 ggatccggac gcaccgcaac aaccggggaa gtatttctgg tttagagcag tgtcaagagg
     7141 gatttctcag cccctcactc accggctcag tctcagggca ggagcattaa tacgctcagt
     7201 tcatgtctta tacaggaatg ggcacagggg gagctggcgt gtcactgctg attttacgca
     7261 tcagcgttca tgtatctgca ctctgataca tgaacgattc acagcgctct gttaccggag
     7321 agatttactc tcttagctta ctctggcaaa tcctttacgt tactctctga tgacttctta
     7381 tatttataat accaatcaga cagccttatc ccatcggtga tatcagtctc ctcctcgcca
     7441 ggaacgggct gactcactgt aactacctcc gaaaacaact ctgcaattat cagaacgcct
     7501 gcttctctcc ctgtcctcac gaaaactatc ccctctttat cgcgcgtgcg tgcggaagca
     7561 tcttttcgca acaaccaccc ggtataccgg tacggctcag ccaccgcaac ctccccgttt
     7621 atctcctggc ggtcacattc ccgattattt tttacgtttc tccccggttg ttatgccggt
     7681 gaaggtggcg cgctgttttc atgaccacac cggctggttt aacaacatcc ggaggaacat
     7741 tctcatggct accccagttt cactgatgga tgaccagatg gtcgacatgg catttatcac
     7801 tcaactgacc ggcctgacag ataagtggtt ttacaggctc atcagggatg gggcctttcc
     7861 ggctcccatc aagctgggcc gcagctcccg ctggctgaaa agtgaagtgg aagcctggct
     7921 acaggcgcgt attgcacagc cccgcccgta atttctgacc gttatccgtt cacccgcagc
     7981 agacgtaatc cccggcctgc cggcggcatt ctgctgcctg ttatatcccc gtgaggaata
     8041 tgaaaatgaa acaacagtat cagacccgct acgaatggct ccacgaaagc taccagaaat
     8101 ggctgaccgg cttcacccgg cacgccgtat cctggggcgt gtgtcatccg aatatctact
     8161 acttccataa tctgacgccc ggttgggtgt cattcaatgg tgaaaaaccg gagattgcca
     8221 tcgtacccca gagcctgcac cggctgattt atggccctga caaacgggcc acgccgcccc
     8281 tggatgatga tctgatagtg aatttatgca ccagtgaaca tctgctggtt catcatccga
     8341 tgctggaagg cattctcctg tctgaatgtg aacgtctgag gcagcgttct ctggcaaata
     8401 aactgatcag cctcttccgt cagtttggtg gcacggagtt gcgcctcaaa ctggtctggc
     8461 tttgctggct tgatttaatg accggaaaca gccttgaaga ctggaaagag aacctgaaac
     8521 ggaaatcaga aaaagagctg gaggaatgga tcatcaaccg tcagaggcag agcgcagcac
     8581 tgacggatct gatggatcag tacgtgctcc tggcataccg cacaacggtt gacgatagcc
     8641 gcaactgatg ccggttgctt ccggaaggtg tgacaaaata cggaaatgat gatgctcccg
     8701 gcccccatgc tcacctgcag agcgtgttcg tcgtcatcat actgagccat gacgggcgtc
     8761 ttaagggcat cctgtcattg ctgagcaggc aacaccacca gaggagccac ggacagggat
     8821 gttctttaca gcccgataca cgccaggttc tcagcagact gaatgtgcag gtgcggttat
     8881 tcaccagaaa tggcatgctg cggtacatac ccatcctggt gacaatatgt acgtggcgac
     8941 agcgatcccg gcactgtgga agagactcac tcagcctgca ggatattctt tccggcatcc
     9001 gggaatgtcc gcctgaatgc aaacaggatg atatttgaat ccgtaatact gttctttcat
     9061 cataaataat ttatgcagat acagttaacc gttacagtag ccggtttgtt ttaccgggaa
     9121 ataatatccg tcagactgat accggaacat atctgaaaca cgttatgatg ttttgttatc
     9181 agaaatgttg tatattctta tccgtggcga cagtttaatg ccctctggta tcacctgata
     9241 atgtatatcc tgcgatgatt cgttatattt acgggacacg cctcttttca ttgtgctcct
     9301 tttcagactt cattttccgt aagtgttccc cggcataaag gaggtacctt tctggcgttt
     9361 ataactctct ttatcggtga cgccggtaat acagggaata ccacaccgac aaacgaggta
     9421 ttaacagacg atatttcagt gtccataatt tttctgtccg gttaaataac gtcagtctgc
     9481 tgccctctga ctgtaatcat caaagccgga atgcataccg ggttacaacg taaaaaaagt
     9541 gatgctggcc cccggtgcag ctggcgggcg aaacatgttt acctttcaat accccctgtc
     9601 tgcagtctcc actcttccat cctcttaagc tacgtcggcc acatgagtgc acttccatga
     9661 taacaggtat tcctgtcagc ataacgaagc cggttacctt cccttcctcc tgcatttcct
     9721 gtcagaatga ctatacagcg cgcagattgc ctttttattg accatcttaa tataccgtta
     9781 taaacttctt tttatcatat tattatcacc ttattatgtt taatttttaa gcagtcggag
     9841 aaagaggatg gttcagaaaa ttttgtcgga taaggttatg aatgaaagaa caaatgcgta
     9901 ttactcttat tatcttggag agcgtaatat ctccgtactg ccccttaatg tttatgatcc
     9961 cccggaacgt tttatcgctt acataaaaaa aaacagagaa aatctgaaca tcacactttc
    10021 agattttgaa cttgaacaaa taatttctgg tatgcgtctg aaggccctgg ccttcctggt
    10081 ccccctggaa aaaatatcct ggatcgcggg cagtgaacgc gcatgcttat tttcctggta
    10141 tctgttgatg cagtttatcc agaataacag ggcaaaaata agcgcagact tgcttcaaaa
    10201 gaataaactg tatcttaaag aagagtatct ggaagggaat gccttcccgt cggactcatc
    10261 gacacagttc agacaaatac tcagagttct cgatattctt tctgataaga atttgcggga
    10321 tgagtggata atccagacca aagacagatg gatgcgggca tttaaatcaa agagtccttt
    10381 cagttacctc ctcccggaga acgaacatga atgcatatgg acatggaatt atttgaaggg
    10441 aaaaaatatt gcactggaaa aactggccag ttttcctggt tctgctgata tttaccacgc
    10501 aattcacctt tcatttgaca tatgggttac ctgtccactc acctcacctg acgatataaa
    10561 aaatttcaga aacagtttca ataaggccaa ggcgcagcgc aagtacaaaa aaatgcagga
    10621 agataaagtc aatgtacagt tttttcttga cgcagaaacc agggcgcagc tcaaagaatt
    10681 atcgagggtc agaagactca gtaccggcga aatgctgcat gacctgattg ttgaagagta
    10741 taaaaggtac cgacattcca gataatttca gacatatatt atctccgtga gtcccccgcc
    10801 acctttccgg tgtgcggggg ttttgtcttt tttcaccggg aatacatgta tgaatccgtc
    10861 tgatgccatt gaggcaattg aaaaaccgct ctcctccctg ccttactcac tttcccgtca
    10921 catcctggaa catctgcgca aactcaccag tcacgaaccc gtgattggca ttatgggtaa
    10981 aagcggggcc ggtaaatcct cactctgtaa tgcactgttt cagggggagg tcaccccggt
    11041 cagtgatgtt cacgccggca cccgggaagt gcagcgtttc cgtctgagtg gccatggtca
    11101 cagcatggtt atcactgacc tgcccggggt gggcgagagc cgggacagag atgcagagta
    11161 tgaagccctg taccgtgaca ttctgcctga acttgacctg gtactgtggc tgattaaagc
    11221 cgatgaccgt gccctgtctg tggatgagta tttctggcga cacatcctgc accggggaca
    11281 tcagcaggtg ctgtttgtgg tgacgcaggc cgacaaaacg gagccctgcc atgaatggga
    11341 tatggccggt attcagcctt ctcccgcaca ggcacagaat attcgcgaaa aaacggaggc
    11401 ggtattccgt ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg
    11461 ggaactggat acgctggtca gtgcgctaat gacagcactt cccgaccatg ccgccagtcc
    11521 cctgatgacc cgcctgcagg acgagctgcg cacggaatct gtccggggtc aggcccgtga
    11581 acagtttacc ggtgcggtgg accggatatt tgacacggcg gagagcgtct gtgttgcctc
    11641 tgttgcccgt acggttctgc gtgccgtccg tgacacggtg gtctctgttg cccgcgctgt
    11701 atggaactgg attttcttct gaacagtctg ccagggaaat gcctgataac attacgttaa
    11761 ccaactgaaa acatgacaca aacccctcat actcctcctg tttctcttgc gtgaccgcct
    11821 tgtcgttaat agaataaaac tatcgataaa acagatatcg atcgttgcta tcgatcgttt
    11881 atagtggtta atggtctgcg aaaaggcagc tgaatctctt catcatgcag aacggaattg
    11941 cacacaacgg actgatactt ctctggcgga tgagagaggg gaagcattta tgtccgggaa
    12001 aaaccacttt atctgaccat gctgttttgt acctgccggt atccactttt gtgggtaccg
    12061 gcttttttat tcaccctctg taaggaaaag ctgatgaaac gacatctgaa caccagctac
    12121 aggctggtat ggaatcacat tacgggcacc ctggtggtgg cttccgaact ggcccgctca
    12181 cggggaaaac gcgccggtgt ggcggttgtg ctgtctcttg ctgctgtcac atcagtcccg
    12241 gcactggctg ctgacacgat tgtacaggcg ggagaaaccg tgaacggcgg aacactgaca
    12301 aatcatgaca accagattgt tctcggtacg gccaacggaa tgaccatcag tagcggtctg
    12361 gagtatgggc cggataacga ggccaatacc ggcgggcaat ggatacaaaa tggcggtatc
    12421 gccaacaaca ctactgtcac cggtggtggt cttcagagag tgaatgccgg aggaagcgtt
    12481 tcagacacgg ttatcagtgc cggaggcgga cagagccttc aggggcaggc agtgaatacc
    12541 actctgaacg gcggtgagca gtgggtacat gaaggcggga ttgcaacggg taccgtcatt
    12601 aatgagaagg gctggcagac cgtcaaatcc ggcgcaatgg caaccgacac ggttgtgaat
    12661 accggcgcgg aagggggacc agatgcagaa aatggtgata ccgggcagtt tgttcgcgga
    12721 aatgccgtac gtaccactat caatgaaaat ggtcgtcaga ttgtggctgc tgaaggaaca
    12781 gcaaatacca ctgtggttta tgccggcggc gaccagacgg tacacgggca tgcgctggat
    12841 accacactga atggcggtta ccagtatgtg cacaacggcg gtacggcgtc tggcactgtt
    12901 gtaaacagtg acggctggca gattatcaag gaaggtggtc tggcggattt caccaccgtt
    12961 aaccagaaag gcaaactgca ggtgaacgcc ggtggtaccg ccacacatgt caccctgaaa
    13021 cagggtggag cactggttac cagtactgcg gcaacggttc ttggcagcaa ccgtctgggc
    13081 aatttcacgg tggaaaacgg taaggctgac ggagttgttc tggagtccgg cggtcgcctg
    13141 gatgtactgg aaggccattc agcgcagaaa acacgggtgg atgacggcgg aaccctggca
    13201 gtgtctgccg gtggtaaggc aacaggtgtc accatgacat ccggtggtgc cctgattgca
    13261 gacagtggtg ccactgttga ggggaccaat gccagcggta agttcagtat tgatggcaca
    13321 tccggtcagg ccagcggact gctgctggaa aatggcggca gctttacggt taatgccgga
    13381 ggactggcca gcaacaccac tgtcggacat cgtggaacac tgacgctggc tgccggggga
    13441 agtctgagtg gcagaacaca gctcagtaaa ggtgccagca tggtactgaa tggcgatgtg
    13501 gtcagtaccg gcgatattgt taacgcaggg gagattcgct ttgataatca gacgacaccg
    13561 gatgccgcgc tgagccgtgc tgttgcaaaa ggcgactccc cggtaacgtt ccataaactg
    13621 accaccagta acctcaccgg tcagggtggc accatcaata tgcgtgttcg ccttgatggc
    13681 agcaatacct ctgaccagct ggtgattaat ggtggtcagg caaccggcaa aacctggctt
    13741 gcgtttacaa atgtcggaaa cagtaacctc ggggtggcaa cctccggaca gggtatccgg
    13801 gttgtggatg cacagaatgg cgccaccaca gaagaaggtg cgtttgccct gagtcgcccg
    13861 cttcaggccg gcgcctttaa ctacaccctg aaccgtgaca gcgatgaaga ctggtacctg
    13921 cgcagtgaaa atgcttatcg tgctgaagtc cccctgtata catccatgct gacacaggca
    13981 atggactatg accggattct ggcaggctcc cgcagccatc agaccggtgt aaacggtgaa
    14041 aataacagcg tccgtctcag cattcagggc ggtcatctcg gtcacgataa caacggcggt
    14101 attgcccgtg gagccacgcc ggaaagcagc ggcagctatg gcttcgtccg tctggagggt
    14161 gacctgctga gaacagaggt tgccggtatg tctctgacga caggggtgta tggtgccgca
    14221 ggccattctt ccgttgatgt taaggatgat gacggttccc gcgccggcac ggtccgggat
    14281 gatgccggca gcctgggcgg atacctgaat ctgacacaca cgtcctccgg cctgtgggct
    14341 gacattgtgg cacagggaac ccgccacagc atgaaagcgt catcggacaa taacgacttc
    14401 cgtgcccggg gctggggctg gctgggctca ctggaaaccg gtctgccctt cagtatcact
    14461 gacaacctga tgctggagcc acaactgcac tatacctggc agggacttac cctggatgac
    14521 ggccaggata acgccggtta tgtgaagttc gggcatggca gtgcacaaca tgtgcgtgcc
    14581 ggtttccgtc tgggcagcca caacgatatg agctttggtg aaggcacctc atcccgtgac
    14641 actctgcgcg acagtacaaa acaccgtgtg agtgaactgc cggtgaactg gtgggtacag
    14701 ccttctgtta tccgcacttt cagctcccgg ggtgacatga gcatggggac agccgccgcc
    14761 ggcagtaaca tgacgttctc accgtcccgg aatggcacgt cactggacct gcaggccgga
    14821 ctggaagccc gtgtccggga aaatatcacc ctgggcgttc aggccggtta tgcccacagc
    14881 gtcagcggca gcagcgctga aggttataac ggtcaggcca cgctgaatgt gactttttga
    14941 taatcaggtc actctccttc aggccccggt catcatgacc ggggcttttt actttcaggg
    15001 agcaacagat atgccgggtt gtacatcacg tttattgccc gaaggccctt tctcacgcga
    15061 acaggctgtt gccgtcaaaa cagcttaccg caatgtgttt actgaagatg accagggaac
    15121 atattcccgg ctggttatcc gtaatgctga aggtcagttg cgctggtgat gccggaacac
    15181 agcttaatac tcagctcgcc agttaagggc ttctcagaca ataaacgtca ttcatttcat
    15241 ctgtcaggcc gcgtcttctc cgggagacgc agctttttca tttattacac cagtaaatcg
    15301 taaccaccga taaggagcaa attatgcgac tagccagtcg ttttggttat gcgaaccaga
    15361 tacgccgtga ccgtccgctg acacacgaag aactgatgca ctatgttccc agtattttcg
    15421 gtgaagaccg gcatacttcc cgcagtaaac gatatgcgta cattcccacc atcaccgtac
    15481 tggaaagcct gcagcaggaa ggttttcagc cattcttcgc ctgccagacc cgcgtgcgcg
    15541 acccgggccg ccggggatac acaaaacaca tgctgcgtct gcggcgggcc ggagagataa
    15601 acggagaaca tgtccctgaa attattctgc tcaactctca tgacggtacc tccagctacc
    15661 agatgctgcc gggttacttc agattcgtct gccagaatgg ctgcgtctgc ggtcagtctc
    15721 tgggggaagt gcgtgttcca caccggggaa atgtggtgga gaaagttatc gaaggggctt
    15781 acgaagtggt gggggttttt gaccggattg aggaaaagcg tgatgccatg cagtcgctgg
    15841 tcctgccgcc accggcacgc caggcgctgg cacaggcggc actgacttac cgttatggtg
    15901 acgaacatca gccagtcacc accgctgaca ttctgacacc acgacgcagg gaggattacg
    15961 gtaaagacct gtggagcgca tatcagacca tccaggagaa tatgctgaaa ggcgggattt
    16021 ccggccgcag tgcaaaagga aaacgtatcc acacccgtgc cattcacaac atcgacaccg
    16081 atattaagct caaccgcgca ttgtgggtaa tggcagaaac gctgctggag agcctgcgct
    16141 gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg ccccggaggc
    16201 gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta cccgtctgct
    16261 ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg catctggcca
    16321 tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc gaatatcatt
    16381 catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc cagctcccga
    16441 cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaaa cattgtctca
    16501 gaacaccacc gcttcggcct gtgcaccgga gactggcctg caacaactgg ttgccactcc
    16561 cgtccctgat gaacagcgca tcagcttctg gccgcagcat tttggcctca ttccacagtg
    16621 ggtcaccctg gaaccccgtg tcttcggctg gatggaccgt ctgtgtgaag actactgcgg
    16681 tggtatctgg aatctgtaca ccctgaacaa cggcggggca tttatggcac ccgaaccgga
    16741 tgacgatgat gacgaaacat gggtactgtt caatgccatg aacggtaacc gcgctgaaat
    16801 gagcccggaa gccgccggca ttgctgcctg tctgatgacg tacagtcatc atgcctgtcg
    16861 tacggagtgt tatgccatga cggtccatta ttaccggctg cgggattacg ccctgcagca
    16921 tccggaatgc agcgccatta tgcgcatcat cgactgaatg gaggcaggga caatgcagca
    16981 gctttccttt ctgcccggaa acatgacgcc cggcgagcgc agtctcattc tgcgggccct
    17041 gcaaaccctg gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca cccgtgcggc
    17101 acgggaatgg ctgattctga acatggcggg actggagcgg gaagagttcc gggtgctgta
    17161 tctgaacaac cagaatcagc tgattgccgg tgaaaccctc ttcaccggca ccatcaaccg
    17221 tacggaagtt catccccggg aagtgattaa acgcgccctg taccacaatg ccgctgccgt
    17281 ggtgctggcg cacaatcacc cgtccggtga agtcacaccc agtaaggcag accggcttat
    17341 caccgaacgt ctggtacagg cactgggcct ggtggatatc cgggtgccgg accatctgat
    17401 agtcggtggc aaccaggttt tctcctttgc cgaacatggt ctgctttaac ccgtcacaac
    17461 cacatcacac ctgttttcac ttttatcttc tgtcttcaga ggtatcacat catgagaatc
    17521 atcacccgtg gtgaagccat gcgtattcac caacagcatc ctgcatcccg tctttttccg
    17581 ttctgtaccg gtaagtatcg ctggcacggc agtgctgaag cgtatactgg tcgtgaagtg
    17641 caggatattc ccggcgttct tgccgtgttt gcagaacgcc gtaaggacag ttttggcccg
    17701 tatgtccggc tgatgagtgt caccctgaac tgatgttccg tcacccggga aacgagtacg
    17761 accagatgac ctcagacgcc gctgtggatg aggccattct catcaatgca tacatcttca
    17821 ccgaaaccgg tcagcgcgcc ggctgacctg tcatcgttaa accattctta accacttttc
    17881 agagaggatt ttatcgtgtc agacaaactc tccgggataa ctcatcccga tgacaatcac
    17941 gaccgcccct ggtgggggct tccctgcaca gtgaggccct gttttggcgc tcgtctggtg
    18001 caagagggta accgcctgta ttaccttgcc gaccgcgccg gtatcagagg ccgcttcagc
    18061 gacgcagatg cataccatct ggaccaggcc tttccgctgc tgatgaaaca actggaactc
    18121 atgctcacca gcggtgaact gaatccccga tatcagcata ccgtcacgct gtatgcaaaa
    18181 gggctgacct gcgaagccga caccctcggc tcctgtggtt acgtttatct ggctgtttat
    18241 ccgacaccag cacccgcaac cacctcataa ccccgaagct ctcaccttta gcactaccag
    18301 ttacagagaa cttaagatga aaacattatc cgacacacat gtacgggagg tatctcgctg
    18361 cccgtctccc gtcaccatct ggcagacact gctcatccga ctgctggacc agcattatgg
    18421 cctcacactg aatgacacac cgtttgtcga tgaacgtgtg attgagcagc atattgaggc
    18481 aggcatttca ctgtgtgatg cagttaattt tctcgtggaa aaatacgcgc tggtgcgtac
    18541 cgaccagccg ggattcagca cctgtccccg ctctcagtta ataaacagta ttgatatcct
    18601 ccgggctcgc cgggcgaccg gcctgatgac ccgcgacaat tacagaacgg taaataacat
    18661 taccctgggt aagtatccgg aggcgaaatg atgaaactgg ccctgacact ggaagccgac
    18721 agcgttaacg tacaggcact gaacataggg cgcattgtcg ttgacgtcga tggtattgag
    18781 ctcgctgaac tgattaacat ggtctgcgat aacggctact cccttcgtgt tgttgatgaa
    18841 tctgaccaga cctcagcaga atgcacgcca ccatttgcta cccttaccgg catacgctgc
    18901 agtaccgcac atatcacgga aacggacaac gcctggttgt actcgctgtc acaccagacc
    18961 agtgacttcg gtgaatcaga atggattcat tttacaggta gcggatatct gttacgtacc
    19021 gatgcgtggt catacccggt tctgcggctt aaacgcctgg ggctgtcaaa aacgttccgt
    19081 cgtctggtcg tcacactcat ccggcgttat ggcgtcagtc tcattcatct ggatgccagc
    19141 gctgaatgcc tgccgggttt acccactttc gactggtaac caggaacaac atgaaatcat
    19201 taaccacgga aacagcgctg gatattctga ttgcgtggct gcaggacaat atcgactgcg
    19261 gatcgggaat tatctttgac aacgatgagg ataaaacgga ttcagcagca ctgttgccct
    19321 gtatcgaaca ggcccgggag gatgtccgta ccctgcgtca tctgcagctt ctgcaccaga
    19381 accggtgaag ctcactcacc agacttcatt cctctgacgc cagcctgcac agggctggcg
    19441 ttttcattta tctgcaaaaa ggaatatcga ttatgtcaga tatcacaatc tcccgtccgg
    19501 aagtggtcaa cgggcatacg gacgttatct gctcaaccag cattcgccac attctggctg
    19561 tacgaaagag tacactgttg caaatcgaca cacttatccg acaactggct gaaatctcgg
    19621 tgctgacgga aagcatcgga ggtaaaactg ccccggactg ggccatgaaa caggattttc
    19681 gctgcggctg ctggctgatg gagaaacccg aaacagcaat gaaagccatt acccggaatc
    19741 ttgatcgcga aatctggcgt gacctgatgc aacgttccgg gatgctgtca ttaatggata
    19801 cgcaggcccg tgatacatgg taccggtcac tggagtacga taattttccg gaaatcagcg
    19861 aagcgaacat tctgagcaca tttgaacaac tgcaccagaa taaggatgag gtgtttgagc
    19921 gaggggtgat caacgtcttc agagggctga actggaatta caaaaccaat tgcccctgta
    19981 aatttggcag taaaattatc gtcaacaatc tggtgagatg ggaccggtgg ggataatgat
    20041 atggtcaccc ccaccttaac agcagtacgc tgttaaggta aatcccttta acggagagac
    20101 catcatgcag aacatcacac ttattggtat cgatctcgga aagcactcct tccacattca
    20161 ttgtcaagac aagtctggta aggctcttct gcgtaaaaaa tttacccgta ctaaactgat
    20221 gcagtttctg gcgacatgtc cgccctcagt tgttgtaatg gaagcctgtg cgggtgcaca
    20281 ctttatggct cgctgtatct ctgattttgg gcatgaaact aagctgattt ctccacagtt
    20341 cgttcgcccg tttgtcaaaa gtaataagaa tgattttgtt gatgcagaag ccatatgcga
    20401 agcggcttca cgaccatcaa tgcgttttgt ccagccaaga actgagaccc agcaggccat
    20461 gagggcgtta caccgggtca gagaatcgct catcagagat aaggtgaaaa caaccaacca
    20521 gatccacggt ttcttgctgg aatttggaat cagcctgccg ataggtgatg ctgtaataaa
    20581 acggttgtct ctcgtgttgg ctgaacacga aatccccgaa tatctgagcc gccttttggt
    20641 gaggttgcat acccactact tttaccttgt tgagcaaata gcagagctgg aatcagaatt
    20701 aagtcagtca atcaacgctg atgataccgc tcagcgcatt atgacaattc ctggtgttgg
    20761 tccgattact gccagcttac tatcgtcgca acttggtgac gggaaacagt tctcatgcag
    20821 cagggatttt gcagcttcca caggacttgt tccccggcag tatagcactg gaggaaaatc
    20881 gactctgttg ggtatcagta agcgaggtga taaaaattta cgccggttac tggttcagtg
    20941 tgcccgctca ttcataatgc gacttgaacg caagcatgga aaacttgctg agtgggttcg
    21001 ggagcaactc aacaagaagc attcgaatgt ggttacctgc gctctggcaa ataaactggc
    21061 tcgaattgcc tgggcaatca ctactcagaa aaaagaatat caggcctgat atagatgctg
    21121 atataactca attagtttat tttaaacaat aagatacaag ttacctttct ggttttgcga
    21181 agactgatta ttgatgacat gaacggcaaa tcgacctgat taaaaaccta acagatgaaa
    21241 tggctcactg aagccgaggg gatttttagg attatcaggt gcggaactca tcggggcgcg
    21301 ggcatccttg cccacagaga cgccggatag atttaagcaa gccaaccatt aatcaaaaat
    21361 cagtgttgca aaaacggggg tgaccataga tttcatctta tcaccggaca acaggctgac
    21421 cgactcgccg atctggaaag aatgttgcac ctgttcagcg gtaaaccaat ccccgacaac
    21481 cggggaaaca tcacccttcg tctggatgat cacattcagt ctgttcaggg taaagagagc
    21541 tatgaagatg agatgttcag catcagatac tttaagaagg gctccgcgca tatcacgttc
    21601 agaaaaccag aactggttga cagactgaat gatattatcg caaaacacta tcctgatatg
    21661 ctggcagtat agctttgcca gtatgctcaa agatcggaaa ttgtccgata agatatgatc
    21721 gcggggcata taaaccggtt tcagatggca tacccgatgg catactgtct ggaaggaaaa
    21781 ataaaaaagc gcatgttaaa caataacatg cacttataat ctggcggtga gggggggatt
    21841 cgaacccccg atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga
    21901 cacctcacca aattgtcgtt cctgtcttgc tggaacgggc gctaatttag ggaaatcatg
    21961 acctgaggtc aacaaacttt ttgaaaaaat cgcgcgttta ttcaaacatt caatcaatgt
    22021 gtggttttaa taagcgaaat ctgctttttt tgccaccgac cacggatttg ttatgctggt
    22081 ggcctttgta gatcataacg ataagtgcga ataaatttcg cacaacgctt ttcgggagtc
    22141 agtatggata tcatctttta tcacccaacg ttcgataccc aatggtggat tgaggcactg
    22201 cgcaaagcta ttcctcaggc aagagtcagg gcatggaaaa gcggagataa tgactctgct
    22261 gattatgttt tagtctggca tcctcctgtt gaaatgctgg cagggcgcga tcttaaagcg
    22321 gtgttcgcac tcggggccgg tgttgattct attttgagca agctacaggc acaccctgaa
    22381 atgctgaacc cttctgttcc actttttcgc ctggaagata ccggtatggg tgagcaaatg
    22441 caggaatatg ctgtcagtca ggtgctgcat tggtttcgac gttttgacga ttatcgcatc
    22501 cagcaaaata gttcgcattg gcaaccgctg cctgaatatc atcgggaaga ttttaccatc
    22561 ggcattttgg gcgcaggcgt actgggctgt aaagttgctc agagtctgca aacctagcgc
    22621 tttccgctgc gttgctggag tcgaacccgt aaatcgtggc ctggcgtgca aagctttgcc
    22681 ggacgggaag aactgtctgc atttctgagc caatgtcggg tattgattaa tttgttaccg
    22741 aatacccctg aaaccgtcgg cattattaat caacaattac tcgaaaaatt acctgatggc
    22801 gcgtatctcc tcaacctggc gcgtggtgtt catgttgtgg aagatgacct gctcgcggcg
    22861 ctggatagcg ggaaagttaa aggcgcaatg ctggatgttt ttaatcgtga acccttaccg
    22921 cctgaaagtc cgctctggca acatccacgc gtgacgataa caccacatgt cgccgcgatt
    22981 acccgtcccg ctgaagctgt ggagtacatt tctcgcacta ttgcccagct cgaaaaaggg
    23041 gagagggtct gcgggcaagt cgaccgcgca cgtggctact aataaagcat caggattcct
    23101 gctatccttg gcgggaattg aatacaggag agagttatgt atcccgtcga ccttcatatg
    23161 cataccgttg ccagcacaca tgcatatagc acattgagtg attacattgc ccaggccaaa
    23221 caaaagggca ttaaactttt tgcgatcacc gatcatggcc cggatatgga agatgcgccg
    23281 catcactggc acttcattaa catgcgtatc tggccgcgag tggttgatgg ggtagggatc
    23341 ctgcgcggca ttgaagctaa cattaaaaat gttgatggtg aaattgactg cagcggtaaa
    23401 atgtttgact cgctggatct gattattgcc ggttttcatg agccggtttt tgcgccacat
    23461 gacaaagcga ccaatacaca agcgatgatc tccactatcg ccagcggcaa tgtgcatata
    23521 ataagccatc ccggaaatcc gaaatatgaa atagatgtta aagccgttgc tgaagcagcc
    23581 gcgaaacatc aggtggcgct ggaaatcaat aattcctcat ttttacactc acgtaagggc
    23641 agtgaagaca actgtcgtgc ggtagctgca gcggtacgtg atgctggagg ttgggtggca
    23701 ttaggctcgg attctcacac cgcgtttacc atgggggaat ttgaagagtg tcttaaaatc
    23761 ctcgacgcgg tagattttcc gctagagcgc attttgaatg tttctccgcg ccgcttactg
    23821 aacttccttg aatctcgcgg tatggcaccg attgcggaat ttgcagacct ttaattacta
    23881 gtaatggaaa tatattaatg aacgagtttt ctatcctctg tcgtgtgctg ggttcgctct
    23941 attaccgcca accgcaagat cctttactgg tgccgctgtt tactctgatt cgtgagggga
    24001 aactggctgc gaactggcca ctggagcagg atgagttact gacgcgttta cagaaaagct
    24061 gtgatatgac gcaagtctct gccgattaca atgcgttgtt tatcggcgat gaatgtgccg
    24121 tgccgccata tcgtagcgca tgggttgagg acgcaacgga agcggaagtg cgcgcttttc
    24181 tttccgaacg agggatgcca ctggcggata cgccagccga tcacatcggc acattgctgc
    24241 ttgcggcttc ctggctggaa gatcagtcaa cggaagatga gagcgaagca ctggaaacac
    24301 tgttcagtga gtatctgtta ccctggtgtg gtgcgttcct cggcaaagtg gaggcccatg
    24361 caaccacgcc tttctggcgc accatggcac cgctaacccg tgatgccatt agtgcaatgt
    24421 gggacgagct ggaagaagat tctgaagagt aaatgtgatc atcgtcactc ataactgcaa
    24481 cagataattt gttgttgcat aaaatgtgtg ctcgatctca ttcatggccg cgttttctgc
    24541 tatcatgcgc ggcatgaaca tacttctctc tattgcaatc acaacaggca ttctctccgg
    24601 tatctgggga tgggtggctg tttccctggg cttactaagc tgggcgggct tcctaggctg
    24661 tacggcctac tttgcttgcc cgcaaggtgg gctgaaaggg ctggcgatct ccgctgcaac
    24721 cctgcttagt ggcgtggtgt gggcgatggt cattatttac ggtagtgcac tggcaccaca
    24781 tctggaaatt ctcggttatg tcataaccgg tattgtcgcg tttctgatgt gtattcaggc
    24841 caaacagctg ctgctttcat ttgttccggg gacgtttata ggcgcatgcg cgacgtttgc
    24901 cgggcagggt gactggaaac tggtgttacc ttcgctggcg cttgggctgg tatttggtta
    24961 cgcaatgaaa aacagcggcc tgtggctggc ggcgcgtagt gcaaagacgg ctcaccgtga
    25021 gcaggaaatc aaaaataaag cgtgaggggc actcacgctt tcgcttaaac agtaaatgcc
    25081 ggattattaa ttccggcatt tttatctgtc aggattccgg tggaaccgac atatggcggt
    25141 atttcaccag aatgtcattc tgccgttctg ctttatttta caaatcccac agaccacggt
    25201 cgataccatc attaatcagg aaaatgaccc ctgtttcgat agccgacatc agacacagca
    25261 taacgggttc gtttgaggtg taacccactt ccccttccag caagcgctgg tagtcaataa
    25321 agcggaaaac cccggcctga acttcatagg aaagtatcgt cttactggtg ttcaccgagg
    25381 agaggatctc accggtactc acattgacga cacgcaggtt cacggcaatc tgatcgagct
    25441 ggtattgcgt gtctgcaccg atcccaaaat atcttgcccc aacaccgccc gatttaacgt
    25501 tgctttcata accgataatc gaaccttcaa ccataatatt tgccgccgtt aaagattgca
    25561 gcgggattcg gttattaatg gcaaccgtgc cgttttcttg tgccgcacga ataaccttgc
    25621 gctcgttaag caggttttgt aagccctggc gctttgcgga acagcagtgg agaagttact
    25681 tgccgggtag ggtttaaatt gcccggtttc gtcctgaatg ttgtataccg aaacaaagat
    25741 cttacccgtc ggcgctggca gatgggtcaa atctttgtag ctctgagcac gaggcattaa
    25801 tgtcggtctg gcggcttctt taggcggggc ggttaagcat ccgctcagta acatgacggc
    25861 aaccaaaaga aataagcgct gcatgattat tttccttatg aagctggggc ttaaaaatcg
    25921 gttgagttat tttgtaaacc cgaaacctgg atggtcgagg tttgtccggt tttacgatct
    25981 gtcacgttca actgcaattg accatcgcgg ttagcaatat caacaatata atcgttggtc
    26041 accatgcggc ccggtttacc ggtattaata ttcgacagta gcccacctaa aatttgtgac
    26101 tggatggcct gagtaaagtt atctaacgct gagggtgttt caataccaaa gtcatcgtta
    26161 tagctcggat ctttataaga gttttgggcc tgagcgctat ttaataaaaa agcgccatta
    26221 tttgggttac caccaaagtt tggattacgg aactggaaag tcatggttcc agcccaactt
    26281 aatggcgaaa taagcatgag tagaactact gcatgtttga cacgcattgc agcctccgaa
    26341 caatttttta tttagaattc atcatgcgcc aaatcgtccg tacttaataa cgcctgattt
    26401 atctggcgac gatttagtgc ttcttcagtt tgaatcagtg caaagacgac agttttctcg
    26461 aagtctcttt tcaacgggaa taaaaaagtc tggaaaataa catcctgatt gaccgttatt
    26521 gtgatccagc ttccccatcg tgcactgggc ctttcattaa tcgttaagtt acccgtatag
    26581 tcactttccc atttatcact aaaggctcgg taaaaatcat ggccaataga tgaaacagta
    26641 tggtcagtta acaatcccgg gacttctacc tcaacggcgt gaagattccc tgcggcgaac
    26701 agaaattctg ccgccacaat ccagcgtaaa taacgtttca tggctttatc gcctgaggtt
    26761 atcgtttgcc caggaaaccg cttgtgtccg gttttttacg gctatcttct tgaaaagatt
    26821 ataaagatgc gttttaaccg tattttcgct gatgaacaac gaacgagcga tctcgttatt
    26881 agacgcgccg atacgcagct tattcaggat ctctttttcc cgatgagtaa ggagggctga
    26941 ttccgtgctg ttataacgat agttaccaga atgtgtaatc aggtaactgg caagcttttg
    27001 cgtaaagtag cattcgccac gcaggacgcc ttgcaaccca ttgacaacac gttcttgatc
    27061 ctccatggca taaaaaacgc cgttgatatg aggccagttt tcaatgtcgc ggtacgggta
    27121 atcttcaggc gtatttagca acaatatttt gatattgttg tttttcctgc tcaaagtatc
    27181 ctgccaataa tggataagct ttttatccgc ttccatcata tccagaagaa taatagagcc
    27241 tgaagagata tcgtccagag aacgttgaat attatgtaat tttcctgtaa ttgctagcga
    27301 ttgtttaagg tactgcaaga gagctgtcgc ctgcaaagaa ggtttagtga tcaacaataa
    27361 tgtatgacca tgaatactat ggacttcatt aaacatgatg aaaccccact tttttattga
    27421 tcgcacacct gacagctgcc tctaaaatag aagcaccaga agtactgaca gatgttgcac
    27481 tgctgtgtgt agtaataaat cagccctaaa tgggtaaaat ataaaactaa tggattacat
    27541 ctgatttcaa tctagccatt acaaatctta aatcaagtgt taaacatgta acaaaatgta
    27601 actcattata ttaaaatgtt aacattaagg ttttattaag tttagaaatg atagaaaagt
    27661 tgtacatttg gtttttattg cacaatttta aaaaatcata caaatggtgc taacttacta
    27721 ataatacata taaaaatatt tcggtgtagt cctttcgtca tgtaaaacgt ttttgttttt
    27781 tctccacacc tccgtggaca attttttact gcaaaaagac gaggtttgtc acggcttgtg
    27841 cgcaagacat atcgcagcaa tcagcgacgg gcaagaagaa tgactgtctg gtgatttttg
    27901 atagcggaaa acggagattt aagagaaaac aaaatatttt ttgcgtagat aacagcgtat
    27961 ttacgcgggt tttaatactt tggtatgaac aaaaaaagaa aaatacaacg cgcgggtgag
    28021 ttattaaaaa tatttccgca gacatacttt ccatcgtaac gcagcgttaa caaaatacag
    28081 gttgcgttaa caaccaagtt gaaatgattt aatttcttaa atgtacgacc aggtccaggg
    28141 tgacaacatg aaaaacaaat tgttatttat gatgttaaca atactgggtg cgcctgggat
    28201 tgcagccgca gcaggttatg atttagctaa ttcagaatat aacttcgcgg taaatgaatt
    28261 gagtaagtct tcatttaatc aggcagccat aattggtcaa gctgggacta ataatagtgc
    28321 tcagttacgg cagggaggct caaaactttt ggcggttgtt gcgcaagaag gtagtagcaa
    28381 tcgggcaaag attgaccaga caggagatta taaccttgca tatattgatc aggcgggcag
    28441 tgccaacgat gccagtattt cgcaaggtgc ttatggtaat actgcgatga ttatccagaa
    28501 aggttctggt aataaagcaa atattacac
//
LOCUS       DAJTRB010000051        28402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTRB010000051 DAJTRB010000000
VERSION     DAJTRB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.051"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO330_002322"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO330_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5883166.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OO330_002323"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OO330_002323"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5883167.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OO330_002324"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OO330_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5883168.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OO330_002325"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OO330_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5883169.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OO330_002326"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OO330_002326"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5883170.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OO330_002327"
     CDS             6426..7055
                     /locus_tag="OO330_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5883171.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO330_002328"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO330_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5883172.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO330_002329"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO330_002329"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5883173.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OO330_002330"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OO330_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5883174.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OO330_002331"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OO330_002331"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5883175.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14054..14815
                     /gene="adiY"
                     /locus_tag="OO330_002332"
     CDS             14054..14815
                     /gene="adiY"
                     /locus_tag="OO330_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5883176.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14952..16313
                     /gene="adiC"
                     /locus_tag="OO330_002333"
     CDS             14952..16313
                     /gene="adiC"
                     /locus_tag="OO330_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5883177.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKDKFPLI
                     QQT"
     gene            16393..18036
                     /gene="eptA"
                     /locus_tag="OO330_002334"
     CDS             16393..18036
                     /gene="eptA"
                     /locus_tag="OO330_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5883178.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18033..18701
                     /gene="basR"
                     /locus_tag="OO330_002335"
     CDS             18033..18701
                     /gene="basR"
                     /locus_tag="OO330_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5883179.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18702..19802
                     /gene="pmrB"
                     /locus_tag="OO330_002336"
     CDS             18702..19802
                     /gene="pmrB"
                     /locus_tag="OO330_002336"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5883180.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19778..19867)
                     /gene="pmrR"
                     /locus_tag="OO330_002337"
     CDS             complement(19778..19867)
                     /gene="pmrR"
                     /locus_tag="OO330_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5883181.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19979..21481)
                     /gene="proP"
                     /locus_tag="OO330_002338"
     CDS             complement(19979..21481)
                     /gene="proP"
                     /locus_tag="OO330_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5883182.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21745..22623)
                     /locus_tag="OO330_002339"
     CDS             complement(21745..22623)
                     /locus_tag="OO330_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5883183.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22620..24848)
                     /gene="crfC"
                     /locus_tag="OO330_002340"
     CDS             complement(22620..24848)
                     /gene="crfC"
                     /locus_tag="OO330_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5883184.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIVERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25250..25585
                     /locus_tag="OO330_002341"
     CDS             25250..25585
                     /locus_tag="OO330_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5883185.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25743..26186
                     /gene="yjdN"
                     /locus_tag="OO330_002342"
     CDS             25743..26186
                     /gene="yjdN"
                     /locus_tag="OO330_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5883186.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26319..27107
                     /gene="phnC"
                     /locus_tag="OO330_002343"
     CDS             26319..27107
                     /gene="phnC"
                     /locus_tag="OO330_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5883187.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27132..28148
                     /gene="phnD"
                     /locus_tag="OO330_002344"
     CDS             27132..28148
                     /gene="phnD"
                     /locus_tag="OO330_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5883188.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28254..>28402
                     /locus_tag="OO330_002345"
     CDS             28254..>28402
                     /locus_tag="OO330_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5883189.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7295 a         7422 c         6936 g         6749 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggttgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggctggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgcaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctgg atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccgcgctttt tttgcctgtt atttatcctg taaaaaaata tgtacatgag aaattactat
    13921 aaaaatttgt aatattagta aaactcgtta tttttatgca tgtttatatt catcatgcaa
    13981 ttatataacc atttcccggt atcgctttgc tttagcgaga actggtcttt tattcgcact
    14041 caggagtaca tgtatgagga tttgcagcga ccaaccttgt attgttttat tgactgaaaa
    14101 agatgtctgg ataagggtga atgggaaaga acctattagc cttaaagcta accatatggc
    14161 gttattaaat tgtgaaaata atattatcga cgtctcctct cttaacaaca ctttggttgc
    14221 tcatattagt cacgacatca tcaaagatta cctccggttt ctgaataaag atctctcgca
    14281 aataccagta tggcaacgta gcgctacgcc catactcacc ctgccatgcc tgacgccaga
    14341 cgtctttcgc gttgccgcgc aacacagcat gatgcccgca gaaactgagt cagaaaagga
    14401 acgaacacgc gcattattat tcacggtgct atcccgtttt ctcgacagta aaaaattcct
    14461 ttcattaatg atgtatatgt tacgtaattg tgtaagtgac agcgtttatc aaattattga
    14521 aagcgatatt cacaaagact ggaatcttag tatggtagcc agttgtttat gtcttagccc
    14581 aagtctgtta aagaaaaagt tgaaaagcga aaacaccagt tatagccaaa taatcaccac
    14641 ctgccgcatg cgttatgccg taaatgaatt aatgatggac ggtaaaaata tctctcaggt
    14701 atcacagtcc tgcggctaca acagtacgtc gtactttatt tctgtcttta aagacttcta
    14761 cggtatgacg ccgctgcatt atgttagtca gcacagagaa cgcactgtcg cctgattttt
    14821 aaccttaacg aagagctata ttaataacgg catcagcgat aacccggtcg ataataattc
    14881 aactatcgaa tgcaggcgta tgatatgacg taatttattg tcacgaagct cgccttcgca
    14941 ggagtttaat tatgtcttcg gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc
    15001 tgatggtgtc ggggaatatt atggggtcag gtgtttttct gttacctgca aacctggcct
    15061 ccactggcgg gattgccatt tatggatggt tggtgacgat tatcggtgcg ctggggctct
    15121 cgatggtata cgccaaaatg tcgttcctcg acccaagtcc tggtggttct tacgcttacg
    15181 cccgccgctg ctttggcccg tttctcggtt atcaaaccaa cgtcctctac tggctggcct
    15241 gctggatcgg caatatcgcc atggtggtca ttggcgtagg atatttaagt tacttcttcc
    15301 cgattctgaa agatccattg gtattaacca tcacctgcgt cgtggtgctg tggatcttcg
    15361 tcctgctgaa cattgtcggt ccgaaaatga tcacccgcgt gcaggccgtt gccactgtgc
    15421 tggcgctgat ccccatcgtc gggattgccg tatttggctg gttctggttc cgtggtgaaa
    15481 cctatatggc ggcatggaac gtcagcggcc tgggcacctt cggtgcgatt caaagtaccc
    15541 ttaacgttac gctgtggtcg ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg
    15601 tgaaaaaccc gaaacgcaat gtccctatcg ccaccattgg tggggtattg attgccgccg
    15661 tttgctatgt gctctccacc accgcgatta tggggatgat ccctaatgcc gcactgcgcg
    15721 tttctgcctc gccattcggt gatgccgcac ggatggcgtt gggtgacacc gctggggcca
    15781 ttgtttcctt ctgcgcagct gcgggttgct taggttcact gggcggctgg acgttgctgg
    15841 cgggtcaaac ggcgaaagcc gctgccgatg acggactgtt cccaccgatt tttgcccgtg
    15901 taaataaagc gggtacgcca gtggcggggc tgattatcgt cggtattttg atgaccatct
    15961 tccagctcag cagcatttca ccaaacgcga ccaaagagtt cggtctggtt tcttccgtct
    16021 cggtcatctt tacactggtg ccatatcttt acacctgtgc ggcgttactg ctgctcggcc
    16081 acggtcactt tggtaaagca cgcccggcat atctggctgt tactaccatt gccttcctct
    16141 actgcatttg ggccgtggtg gggtccgggg cgaaagaggt tatgtggtca tttgtcaccc
    16201 tgatggtcat caccgccatg tatgcgctga attacaaccg gctacataaa aacccgtatc
    16261 ccttagatgc accaataagc aaagataaat tccccttaat ccagcaaaca taaaagccaa
    16321 ccttaagaac ttaaggttgg cttaattttg ctttgcgagc atatgcgcac tttgttcgat
    16381 ggaaacaccg tgatgttgaa gcgcctacta aaaagaccct ctttgaattt actcgcctgg
    16441 ctattgttgg ccgcttttta tatctctatc tgcctgaata ttgccttttt taaacaggtg
    16501 ttgcaggcgc tgccgctgga ttcgctgcat aacgtactgg ttttcttgtc gatgccggtc
    16561 gtcgctttca gcgtgattaa tattgtcctg acactaagct ctttcttatg gcttaatcga
    16621 ccactggcct gcctgtttat tctgattggc gcggctgcac aatatttcat aatgacttac
    16681 ggcatcgtca tcgaccgctc gatgattgcc aatattattg ataccactcc agcagaaagt
    16741 tatgcgctga tgacaccgca aatgttatta acgctgggat tcagcggcgt gcttgctgcg
    16801 ctgattgcct gctggctaaa aatcaaacct gccacctcgc gtctgcgcag tgttcttttc
    16861 cgtggagcca atattctggt ttctgtactg ctgattttgc tggtcgccgc actgttttat
    16921 aaagactacg cctcgttgtt ccgcaataac aaagagctgg tgaaatcctt aagcccctct
    16981 aacagcattg ttgccagctg gtcatggtac tcccatcagc gactggcaaa tctgccgctg
    17041 gtgcgaattg gtgaagacgc gcaccgcaac ccgttaatgc agaacgaaaa acgtaaaaat
    17101 ttgaccatcc tgattgtcgg cgaaacctcg cgggcggaga acttctccct caacggctac
    17161 ccgcgtgaaa ctaacccgcg gctggcgaaa gataacgtgg tctatttccc taataccgca
    17221 tcttgcggca cggcaacggc agtttcagta ccgtgcatgt tctcggatat gccgcgtgag
    17281 cactacaaag aagagctggc acagcaccag gaaggcgtgc tggatatcat tcagcgagcg
    17341 ggcatcaacg tgctgtggaa tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg
    17401 cctcaccaga acgtcaccgc gctgaatctg cctgatcagt gcatcaacgg cgaatgctat
    17461 gacgaagtgc tgttccacgg gctggaagag tacatcaata acctgcaggg tgatggcgtg
    17521 attgtcttac acaccatcgg cagccacggt ccgacctatt acaaccgcta tccgccgcag
    17581 ttcaggaaat ttaccccaac ctgcgacacc aatgagatcc agacctgtac caaagagcaa
    17641 ctggtgaaca cttacgacaa cacgctggtt tacgtcgact atattgttga taaagcgatt
    17701 aatctgctta aagaacatca ggataaattt accaccagcc tggtttatct ttctgaccac
    17761 ggtgaatcgt taggtgaaaa tggcatctat ctgcacggtc tgccttatgc catcgccccg
    17821 gatagccaaa aacaggtgcc aatgctgctg tggctgtcgg aggattatca aaagcggtat
    17881 caggttgacc agaactgcct gcaaaaacag gcgcaaacgc aacactattc acaagacaat
    17941 ttattctcca cgctattggg attaactggc gttgagacga agtattacca ggccgcggat
    18001 gatattctgc aaacttgcag gagagtgagt aaatgaaaat tctgattgtt gaagacgata
    18061 cgctgttatt gcagggactg attctggcgg cgcaaaccga aggctacgcg tgcgatggcg
    18121 tgacaaccgc gcggatggcg gaacaaagcc ttgaggacgg tcattacagc ctggtggtac
    18181 tggatttagg gttacccgac gaagatggac tgcattttct cgcccgtatc cggcagaaaa
    18241 aatacaccct gccggtactg atcctcaccg ctcgcgatac gctgaccgac aaaatcgccg
    18301 ggctggatgt cggtgccgat gactatctgg tgaagccttt tgcgctggaa gagttacacg
    18361 cccgtatccg cgccctgcta cgacgccata ataatcaggg cgaaagtgag ctgattgttg
    18421 gcaatctgac gctgaatatg ggtcgccgtc aggtatggat gggcggtgaa gagttgattc
    18481 tgacgcccaa agaatatgct ctgctgtcac ggttaatgct caaagcaggc agtccggtac
    18541 atcgggaaat tctctacaac gacatctata actgggacaa tgaaccctcg accaacaccc
    18601 tggaagtgca tatccacaat ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc
    18661 gcggctttgg ctatatgctg gtcgcgaatg aggaaaacta attgaatctg atgcgttttc
    18721 tgcgccgacc aatatcgctg cgccaacggc tgatattgac catcggggcc attttgttgg
    18781 tgtttgagct gatcagcgtc ttctggctat ggcatgaaag taccgagcag attcagctgt
    18841 ttgagcaggc gctacgcgac aatcgcaaca acgatcgcca catcatgcgt gagatccgcg
    18901 aggcggtcgc cagcctgatt gtccccggcg tctttatggt cagcctgacg ctatttatct
    18961 gctatcaggc ggtacgccgc atcacccgcc cgctggcgga gctgcaaaaa gagctggaag
    19021 cgcgcaccgc cgacaactta acgcccattg ccattcacag cgccaccctc gaaatcgaag
    19081 cggtggtttc ggcgttgaac gatctggtca gtcgcctgac cagcacgctg gataacgaaa
    19141 ggttgtttac cgctgacgtc gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc
    19201 atctggaact gctggcgaaa acgcatcaca ttgatgtagc accgttagtg gcacggcttg
    19261 atcagatgat ggagagcgtc tcccagctgc tgcaactggc gcgtgccgga cagtcatttt
    19321 cttccggtaa ttatcaacat gtaaaactgc tggaagatgt cattctcccc tcgtatgacg
    19381 aactcagtac catgctcgac cagcgacagc aaaccctgct attgccagag agcgccgccg
    19441 acatcaccgt tcagggtgat gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa
    19501 acgcccatcg ttacagcccg caaggcagca acattatgat taagctgcaa gaagatggcg
    19561 gagcggtcat ggcagttgaa gatgaaggac caggtattga tgagagtaaa tgcggggagt
    19621 tgagtaaagc gtttgtacgt atggacagcc gttatggcgg gattggtctg gggttaagta
    19681 ttgtcagccg cattacacag ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga
    19741 cttccggcac gcgggcctgg gtacggctga agaaagatca gaacgtggca aaccagatat
    19801 aaagaaagct gctgaccacc agcacgctga acacggtagt taaactttca taaacacggt
    19861 ttttcattgc actctcctcc gttaacctgg aggagagtat gcgcgtcaga gattaagtga
    19921 accttaagag ttcaattggc ctgataagac agcgtcacat caggccatcc gtttcagctt
    19981 attcatcaat tcgcggatgt tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa
    20041 tctcgtggtc aatattatcg attttctgct cgatattatc gtaatgctcg acgagaattt
    20101 ccttcgcttc ctgtatatct gacgccgccg gtgtcgcacc tttcaacgga cgatttgccg
    20161 tctctttcat ggttacgccg gtgattaaac caatcaccgc cactaccatc aggtaatagg
    20221 caggcatcat cagattctgc gagctttcga ccagccaggc cgccagtgtt ggcgtcagac
    20281 cggcaaccag caccgaaata ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg
    20341 ggaacatcgc tggcaaggta gaagccataa cgcccataaa gcagttaagg atcaccgcca
    20401 gcatcagtaa cccggcaaaa atcaggccga tgacgttact gttaatcaga ataaacgccg
    20461 ggatcgccaa cacaaacagt gcaacactac caagtagcac aaacggacga cggccaaaac
    20521 ggtcactcag caagcccatc accggctgga caaacagcat accgatcata atggcgataa
    20581 taatcagcac cccgtggtct tcggagtaat gcaggttatg cgacaaataa ctcggcatat
    20641 aggtcagcaa catgtagtaa gtcacgttgg tggcaattac cagaccaata catgtcaaca
    20701 ggctgcgcca gtatttagtg gcaatctctt taaacgagac tttcgggcca tcctgcaaac
    20761 cttcgcggtc gccctgttcc agtttatcaa catgctgctg gaacgccgga gtttcttcca
    20821 gcgcatggcg caggtaaagc ccgataatcc ctaacggcag agcaataaag aacggaatac
    20881 gccagcccca gtcgaggaag ttcgcttcgc cgacaatggt cgaaattaac accaccacgc
    20941 ccgcacccag cacaaacccg gcaatagaac cgaagtccag ccagctgccc ataaagccac
    21001 gtttacggtc aggggagtat tccgcaacaa atatcgacgc cccggtatat tcaccaccga
    21061 ccgagaaacc ttgtgccatc ttacagatca acagcagaat cggtgcccaa ataccaatcg
    21121 tgtcgtagga cggtattaag ccaatacaga acgtactgat cgacataatc acaatagtga
    21181 tagcgaggat cttctggcga ccatatttat cgcccaacat accaaagaag agtccgccaa
    21241 gcggtcgaat cagaaaggga acggagaaag tggcaagtgc agcaaccatc tgcacgctgg
    21301 ggtcagcccc cgggaaaaaa actttaccta atgcgtaagc aacaaaacca taaacaccaa
    21361 aatcaaacca ttccattgca ttacccagtg atgctgcggt aatggcttta cgcagtttac
    21421 cgtcatcaat aatggtgaca tcacgaaggg taatcggttt tacttttttc cttttcagca
    21481 tagctttcct cgcagagttg gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc
    21541 gcaaaagggg agcgggaatt gcaggatgca atctctgtaa caagcgtagc gggtttgctt
    21601 acaccctccg gttaaggaat gtacaatcaa accagttgac gcctgaattg ggcagttaat
    21661 gaccttttta ccctaccagt gtttaaattt gtgatcaact tcacacatgg atttcgttta
    21721 ggactcattg atgtaactga ttatttacgc caaatttaca gagaagattt cctcgaccat
    21781 tgcccccacc aaacgctgcg ctgaacagag tcgtggcatc cccagcgcga cagtttgcca
    21841 gcgttgggtc actgaccagc aaacgggttg tttatctgcc gtggtccact ttcccagcca
    21901 acttaaaaaa tcagcatgat ccccgcccgt cggaacggtc gaagaggcaa accactgatg
    21961 gtaatagtga cgcgtagtcg gtgccgcatt gatactctct gccagatgtt gtgtcgcttt
    22021 ctcgcgcaaa tcacccagca acaacaccac cgcttgcggg cttcccagac ggcaggcatc
    22081 gccaaatgca ccccagcgca actcctccag aacgataaaa cagcgcccag gcaacgacca
    22141 ggcgttatac gccccagcac gccagcgggt aaaaatttgc tcgctctgat cacctgcctg
    22201 caccagtagg cctcgttgcg ttaacgcctt ttccttccag cgacaacggg ctgaaaacca
    22261 ctcatacaag gcggcaattt gctgggggag ctccgggaaa gcagagtgct ggcggtgatc
    22321 cattttttgc agcagcgcca gatgctgcgc gatctgcgtc agcgccagat gaccgggatc
    22381 cagcatcgac gccagttttt ccaccattgt cagtcgcagg ctaagcggcg catttaacat
    22441 gccgccgttc gtccatgtct gaagctgcac acgcgaaaga agttcctgca tcagtcgttc
    22501 acgaaactgc tgctgatggg cttgaggatg gcgggcatca tcgccctgcg ccagatccac
    22561 taaaaagcgc ggatgaaccg actccagcac gcgaccgggg ccgtccagta acgtcttggt
    22621 caatatcgtt ctgccgtgaa aagtgtttga atatcatcgc gtaacagctg agcgtcggtg
    22681 taaatccagc tgtgagtcat cacagtctgc tgcaattgct gctgcatcag cctgaccacc
    22741 gattcatttt gttgacgcag agccagactt tcgcgtaaac gcgtctgtaa ttctgtcaaa
    22801 catgaagcga actcagcgaa aaaagtattc atgcctgccg taacagattc atcgacctgc
    22861 tctgccagaa ctttagccat ttgttggcaa tagagatcga cttctgcgct taatgctcgt
    22921 tgcaacacac tgtaatcaac cgtttctgtc ggggatttct catttccccg tccccagtcg
    22981 ggctgattca accagcgcga aaaagtctca cgcacaacgc ctaaacgcgt gctctgctcg
    23041 tccgttgctt cctggcgcga aatgacttca ctgaacagct ggcgagtgtt gaagtgggga
    23101 actacgccgt gaaaaacagg aaaatgaaac ccaggacgaa accctgactc gctcaattcc
    23161 attttgactt gttgctcaat ggggcgaata acatcggtta acacccggca aagggtggat
    23221 tccatctcgg caaaacgcag cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac
    23281 agtgtctcac agcgggtacg aatctcgctt aacggctccg aatcatcctg aaacaaggcg
    23341 gctaactgcg cattcaccgc atcttgctgt tgacgcagaa agtggttggc ggaggtcagg
    23401 gccagctcga tttcatgttt aatctcgccg ctcacctgcg cctgattgag ttgcaatagc
    23461 tgcaaacttt cttcgacctg atggatattt tgccgcaatt gttcacaagc gacgtttaac
    23521 ccgtgcgcac gaaaatccag gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc
    23581 gcagcagatc gcagagcata caatgaggcg ttagcgtaag cggcatgaag caacgcctga
    23641 attggctggg cgaacagcga atcttcccac aactgatcgg cagcatgacg aatatgttcg
    23701 aggtccgcca gatcggcatg acgccagcgc ctgccgagcg cggcatgggc aaaatcttcc
    23761 acccagcgtt gttgctctgg cgctggtaac ttaccgttgt tggctaactc atagcgcgcc
    23821 cgattcgcca ggtagcccca catcgacgac accgggaata tctgctgtgg cgtaatacag
    23881 cctttcatca gcgtcccgga aatcagtgcc cgcacctggt cggcgtcgtc actgttacga
    23941 tcctgttgat cgaacttatt gaccagcaca tacagcggca ccgattgccc caccgccaaa
    24001 atcgcctcac ggacctcttc atcggagatc gatttcagtt gcgtataatc cagcaccgcc
    24061 agtaccgccg aggcgcgtgc cagctgctgg ttaagcattt tttgcagatg cggttgcccg
    24121 gcttcatttg gcccgggggt atccagtaac gtcaactgac cgggataact ttccagcccc
    24181 gccagatgga caaactccac ttcaatcacg ggaatatgct caatggcggc gtaagcagaa
    24241 aaaggaaaat cgacgtccag cgccttcgcc agtcgcacta aatcattcaa acttttcaga
    24301 caatgaaaaa taggctgggc acccagataa tatttttcga aagcgacgcc attttcgatc
    24361 cgctgcataa gcgcacgcat atctttatct atttccagca catcggtcag atgcttaata
    24421 tcgcaatcac gcaggcgctg ttgtaattgt tgaattaaac aatcgattgg cgcgacatgt
    24481 gaaaaatgca gtaccggttc cttttgcccg ggcgtatggc gaataagcgt cggcagcgca
    24541 gtcattgggc gattacgatt aggcagaacc tccgtaccaa caatggcatt aatggtggtt
    24601 gatttccctg ctttcatggt accgacaatt gccagcacca tttccagtcg ggaaatttta
    24661 cacaactcat tattcagcat cgcgtgacgt tcgacgatat taggctgact ccagggtaaa
    24721 gccagttgtg gcgcgtcgtc tccgggtaca gagagaggca ttttttccag taactgcaac
    24781 tgttggcgag aaagctgtaa caggcgttca gcctcctgac ttaactcata cagggtctgt
    24841 gtgtacatag aaaattcttc cttaaagcaa attttgttac ttttatttag ccagattgtt
    24901 tttgagttct gttttcggct tttataatta ctgcaagaaa taattttata tttagtgtgt
    24961 tgttttttat cagaataaat aacgtcttct gatgcgttta aaacgtcaga aagataaaaa
    25021 tatcatgtga ataaaaaaaa gaacaagtag agcattaaca ttatcttaaa taataaatag
    25081 aggcaaaaag attattttct ttttgcgttt cctttcaaat gaaaacgatc gtcgtctaaa
    25141 atcagcagta cccccgacaa actcagggat tttgtgtata attgcggcct ttttcggcaa
    25201 tctgccgttt tttggcgctt ttgccctgct gacttttgag gaaatccaca tgtcattacc
    25261 acactgccca aaatgcaact ccgaatacac ttacgaagat aacggcatgt acatctgccc
    25321 ggaatgtgcc tacgaatgga acgacgcaga acctgcacag gaaagcgacg agctgatcgt
    25381 taaagatgct aacggcaatc tgctggctga cggcgacagc gttaccatca ttaaagatct
    25441 gaaggtgaaa ggtagctctt cgatgctgaa aattggcacc aaagtgaaaa acatccgcct
    25501 ggttgaaggc gaccataaca tcgattgcaa aatcgacggt tttggtccga tgaaactgaa
    25561 atctgagttt gtgaaaaaga actgattgta ttgtgatcgg taagccggat aaggcgctcg
    25621 cgccgcatcc ggcaacgatg ccgactgcct gatgcgacgc ttgcgcgtct tatcaggcag
    25681 cctctcatct gtataaattt cgaactacac ttaactggct tctcttaact gaggtcacca
    25741 tcatgccgtt aagtccctac ctctcttttg ccggtaactg ttccgacgcg attgcctatt
    25801 atcaacgtac gttgggcgcg gaactgctct ataaaatcag cttcggcgaa atgccgaaat
    25861 cagcgcagga cagcgccgag aactgccctt ccggaatgca atttcccgat accgccatcg
    25921 ctcatgccaa cgtgcgcatt gccggaagcg acatcatgat gagcgatgct ataccgtcag
    25981 gaaaagccag ctactccggc tttacgctgg tgctcgattc gcaacaggtc gaagaaggaa
    26041 aacgctggtt tgataacctt gccgctaacg gaaaaatcga aatggcctgg caggaaactt
    26101 tctgggcgca tggctttggc aaagtcaccg ataaatttgg cgtaccgtgg atgattaatg
    26161 tcgtcaaaca acaaccaacg caataacccg ccgggaggcc cgccctcccg cactgtcatc
    26221 gaattcccgt taactcttca tctgttagtc acttttaatt aaccaaatcg tcacaataat
    26281 ccgccacgat ggagccactt ttttagggag gctgcatcat gcaaacgatt atccgtgtcg
    26341 agaagctcgc caaaaccttc aatcagcatc aagcgctgca tgcggttgat ctgaacattc
    26401 atcacagtga aatggtggct ctgcttgggc cgtcgggttc cggcaaatcc acccttttac
    26461 gtcatttaag cggtttgatt accggcgata aatccgccgg tagccatatc gagctactgg
    26521 gccgcactgt ccagcgcgaa ggccgcctgg cccgcgatat ccgcaaaagc cgcgccaaca
    26581 ccggctacat ctttcaacaa ttcaacctgg tgaaccgcct gagcgtactg gagaacgtgc
    26641 tgattggcgc gctcggcagc acgccgttct ggcgcacctg ttttagctgg ttcaccggcg
    26701 agcagaaaca gcgcgcgtta caggcgctga cccgcgttgg catggtgcat tttgcccatc
    26761 agcgcgtttc caccctctcc ggcggccagc agcaacgtgt ggcgattgcc cgtgcgctga
    26821 tgcagcaggc gaaagtgatt ctggccgatg aacccatcgc ctcgctggac ccggaatcag
    26881 cgcgcatcgt gatggacacc ctgcgcgaca tcaaccagaa cgacggcatc accgtggtcg
    26941 tcacgctgca tcaggtggat tacgccctgc gctactgcga acgcatcgtc gccctgcgtc
    27001 aggggcacgt cttctacgac ggcagcagcc aactgtttga taacgaacgt tttgaccatc
    27061 tctaccgcag cattaaccgc atcgaagaga acgcgaaagc tgcctgatat ccccatcatt
    27121 gaggaaaacg aatgaacgct aagataattg cctcgctggc cttcaccagc atgttcagcc
    27181 tcagcaccct gttaaccccg gcacacgccg aagagcagga aaaggcgctg aatttcggca
    27241 ttatttcaac ggaatcacag caaaacctga aaccgcaatg gacgccgttt ttacaggata
    27301 tggagaagaa actgggcgtg aaggtcaacg ccttctttgc cccggactac gcgggcatta
    27361 tccaggggat gcgcttcaat aaagtggata ttgcctggta cggcaacctg tcggcgatgg
    27421 aagcggtgga tcgcgccaat ggccaggtct tcgcccagac ggtcgcggcg gatggatcgc
    27481 cgggttactg gagcgtgttg atcgtcaaca aagacagccc gatcaacaac ctgaacgatc
    27541 tgctggcgaa gcggaaagat ctcaccttcg gcaatggcga tcctaactcc acctctggct
    27601 tcctcgtccc cggttactac gtcttcgcta aaaacaatat ctccgccagc gacttcaagc
    27661 gcaccgtcaa cgccgggcat gaaaccaacg cgctggccgt cgccaacaag caagtggatg
    27721 ttgccaccaa caacaccgaa aacctcgaca agctgaaaac ctccgcgccg gagaagctta
    27781 aagagctgaa ggtgatctgg aaatcgccgc tgatcctagg cgatccgatc gtctggcgca
    27841 agaatctgtc cgaaactacc aaagacaaga tctacgactt ctttatgaac tacggaaaaa
    27901 cgccggaaga gaaagcggtg ctggaacgcc tgggctgggc gccgttccgc gcttccagcg
    27961 acctgcaact ggtgccgatt cgccagctcg cactgtttaa agagatacaa agcgtgaaag
    28021 gcaataaagg gctgaatgag caggacaagc tggcgaaaac caccgagatt caggcgcagc
    28081 tggatgacct ggaccgcctg aacaacgcgt taagcgcgat gagttcggtg agtaaagcgg
    28141 tgcagtaaat cgtaggtcgg ataagacgcc ccggcgtcgc atccgacaat gtgtaggcgt
    28201 tgatgccgga tgcggtgcaa gcaccttatc cggcctacag accggagcca aacatgcaaa
    28261 ccatcaccat cgccccaccc aagcgtagct ggttctcgct tctgagctgg gccgttgttc
    28321 tcgacgtgct ggtcgtctcg tggcagggcg cggaaatggc cccgcttacg ctgattaaag
    28381 acggcggcaa catggcaacc tt
//
LOCUS       DAJTRB010000052        28366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTRB010000052 DAJTRB010000000
VERSION     DAJTRB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.052"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            229..336
                     /locus_tag="OO330_002346"
     CDS             229..336
                     /locus_tag="OO330_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5883190.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            712..819
                     /locus_tag="OO330_002347"
     CDS             712..819
                     /locus_tag="OO330_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5883191.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="OO330_002348"
     CDS             complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="OO330_002348"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5883192.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="OO330_002349"
     CDS             complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="OO330_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5883193.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="OO330_002350"
     CDS             complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="OO330_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5883194.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4614..4802
                     /locus_tag="OO330_002351"
     CDS             4614..4802
                     /locus_tag="OO330_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5883195.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4814..5566
                     /gene="bcsQ"
                     /locus_tag="OO330_002352"
     CDS             4814..5566
                     /gene="bcsQ"
                     /locus_tag="OO330_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5883196.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5563..5922
                     /locus_tag="OO330_002353"
     CDS             5563..5922
                     /locus_tag="OO330_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5883197.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5919..8180
                     /gene="bcsA"
                     /locus_tag="OO330_002354"
                     /pseudo
     CDS             5919..8180
                     /gene="bcsA"
                     /locus_tag="OO330_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8191..10533
                     /gene="bcsB"
                     /locus_tag="OO330_002355"
     CDS             8191..10533
                     /gene="bcsB"
                     /locus_tag="OO330_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5883198.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRRLNPDNE"
     gene            10540..11646
                     /gene="bcsZ"
                     /locus_tag="OO330_002356"
     CDS             10540..11646
                     /gene="bcsZ"
                     /locus_tag="OO330_002356"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5883199.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11705..11881
                     /locus_tag="OO330_002357"
                     /pseudo
     CDS             11705..11881
                     /locus_tag="OO330_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            12209..13957
                     /gene="bcsC"
                     /locus_tag="OO330_002358"
                     /pseudo
     CDS             12209..13957
                     /gene="bcsC"
                     /locus_tag="OO330_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            14039..16027
                     /gene="hmsP"
                     /locus_tag="OO330_002359"
     CDS             14039..16027
                     /gene="hmsP"
                     /locus_tag="OO330_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5883200.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16212..17498
                     /gene="dctA"
                     /locus_tag="OO330_002360"
     CDS             16212..17498
                     /gene="dctA"
                     /locus_tag="OO330_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5883201.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17719..19215
                     /locus_tag="OO330_002361"
     CDS             17719..19215
                     /locus_tag="OO330_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5883202.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(19311..20240)
                     /gene="kdgK"
                     /locus_tag="OO330_002362"
     CDS             complement(19311..20240)
                     /gene="kdgK"
                     /locus_tag="OO330_002362"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5883203.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20472..21239
                     /gene="pdeH"
                     /locus_tag="OO330_002363"
     CDS             20472..21239
                     /gene="pdeH"
                     /locus_tag="OO330_002363"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5883204.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            21309..23369
                     /locus_tag="OO330_002364"
     CDS             21309..23369
                     /locus_tag="OO330_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5883205.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23551..24873)
                     /locus_tag="OO330_002365"
     CDS             complement(23551..24873)
                     /locus_tag="OO330_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5883206.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25284..26297)
                     /gene="yhjD"
                     /locus_tag="OO330_002366"
     CDS             complement(25284..26297)
                     /gene="yhjD"
                     /locus_tag="OO330_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5883207.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26346..27245)
                     /locus_tag="OO330_002367"
     CDS             complement(26346..27245)
                     /locus_tag="OO330_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5883208.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDVTSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            27765..>28366
                     /locus_tag="OO330_002368"
     CDS             27765..>28366
                     /locus_tag="OO330_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5883209.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6768 a         7149 c         7794 g         6655 t
ORIGIN      
        1 gtgacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aagggttccg
       61 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acatcacggg ggccattctt gaacctgaac
      181 aactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt
      421 aagaaaaatg caacgattgc acgcggataa tattctgcgg aatgcgcagc acagagtgaa
      481 gacgtgacgg attccggaac agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt
      541 ccggtgaggc gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga
      601 aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggctaat cttgaaccca
      661 aacaactcaa gaatagccgc gaaacgttgg cattacaaca caggcggcta tatgacgctc
      721 gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
      781 gccagtatga tcgtgaactg gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc
      841 tgccgtaatg gcaatttgcg cccggatcag gccgcagagg ttaaactctg cggccttttt
      901 cgtgcttact gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatct
      961 tggtattgaa taactactgc atttgagttc tcggagaccg gtgctgtttg tggcaaccca
     1021 ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa tcttgccatc aagaacgcga
     1081 accaccagat cggagatagc caggaagctg ctcggttggt caatgacaat cggtgccccc
     1141 tgatgtggtg ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg
     1201 ctagggatat cacgtaggcc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt
     1261 tccggcacca cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg
     1321 tccagttcat caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc
     1381 ggataatggt tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt
     1441 ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc
     1501 gaaccatcaa agcccagcaa aataaccggc agatttgttt gatccatcaa ttcagtctgc
     1561 atgccgccat tttcgcgaac ttctttcaaa aaaccgccga actggccgtt atgtcccatc
     1621 atcaggtgct gggtaaagcc cagtttcgaa aggttatcaa acagatagca gtcgttattt
     1681 gccggttgat acagattagt gtgcgaagtc tgcccgcagc tggcgcgtag taaacggatc
     1741 gccgccgggc cactgtagga ggtggcggag ttaaagttct tgaaatcaat atcgaaatgc
     1801 gaccacagtg gatgcgacat caacccggcg gcttctatat ccgaccagga aagcgaacag
     1861 atgttaatca ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc
     1921 gatttacgtt tcgcctccgc gttatagaaa ttattcagcc aggcgttaag gttcgccgtt
     1981 gttggcggtg cagtttgtgc gggcatatca cccactaccg gtgcgccacc cgtcgccgca
     2041 acggttgccg ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg tccggctggc
     2101 cacaaggaga aacttggtcc cgccagggta agtacgttca gccatagcag tatggcaacc
     2161 acaaaaacgg taatgcgaat ccattgtgac aggaataacc aggccactaa taaaacaaaa
     2221 atggccccaa tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa ataatcggta
     2281 ctgaaccccg ccacctgcga accctggctc attatgcttt ccgggccagg caaccagatg
     2341 tcatgccaga acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg
     2401 ctgtaacgcg gaatgggcat cagcagaaac gcggcaaaca ccaaattgag gagcgggtgg
     2461 aagttaagat atcccgccca caacaggcca aacttaacca gaaaatagaa gttccagccg
     2521 gaaaggccgc gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga
     2581 gtcatttttt ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat
     2641 aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg
     2701 ccagatagcc cagcgggaaa aatatcagtg cgcaaacgat aataatttca atgatatcgc
     2761 tgatggtcat catgatgagc gctccacagc atcatctaac agtcgcatgg gttctggtat
     2821 tcgtcgccag tggcgaccat cgtgctcggc attgatgacc ggtttagaac tttgcgttaa
     2881 aggcagcggc attccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact
     2941 gatttgatca tcttcaaacc agaccatacg gtttgagaaa atgtcgccag taggcaatgg
     3001 gaaaatatga ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac
     3061 cagccgatta ccgccaatgg tcatgatatc gccggtacgg ttagggcgac atagcgtcag
     3121 ggcttgctca acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg
     3181 ggcaggcaag agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt
     3241 ctggaaacca cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg
     3301 tacatagcga ctaaacttct gcccttgcac gctttcgatc atcgtcagac aacgggagag
     3361 tggcgcattc cacggaataa ccatatttgc accgcaggcc aataacaaac gttcatcggt
     3421 ggcgcgcagg ctagcggtat tttcccgcac gaggattttc atcgcactac cgcgctggcg
     3481 acgcagggta tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa
     3541 gaccaccgtc gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa
     3601 cagttgccag tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat
     3661 gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg ctttgaacta atgtccagat
     3721 accattttgt tgctgaacgc taagctgctg acgggcgctg accccttttt cgttgcacca
     3781 gaaggcaata tccagcaaat gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag
     3841 tgaacggtat tcctcaagca acaatgaaaa ttgtttatcg ttattatttc cgggattaat
     3901 taccaaaagc gaacaatggt ttaacctgct ccatttattc attttatcca accatgagcg
     3961 aagccgctcg gcaggaatat tttgccatgc gttatttgcg caaacaagaa taaaaaggta
     4021 attatgggga tcaatagaac actgcaaatc acgggccaaa tagtatagac ctttttcatg
     4081 atttagcatt gaaaataatt ttattttttc cggcccttga gaatcatcta attgaaagat
     4141 tttcgccgga tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc
     4201 aattgtttga ttcgccagac tgatagcatc ttcatggcga tcgacgttaa accaccagac
     4261 gccgcctgct ggcatatgtc gcagctcatc ccataatgat gagataccga tagagaatac
     4321 agggtccaca atgtccctca tgtcaccatt ttgtctgatt atcagtttac tagcgaaagc
     4381 taagaaataa acctaccatt gaaattaaaa cttatcagat ttagcatgtg aatcaattta
     4441 gaaggtcaca agacgtgtga ttttagtctt ttcgttttta actttttctg ttgcgtaacg
     4501 gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac aataaaccgg cgaaatgctc
     4561 acggttagaa ttgtctcatg aatggtacgg ttatttcata gggatcaagc aaaatgaata
     4621 acaatgaacc agatactctg cctgatcccg cgataggcta tatcttccag aatgatattg
     4681 tggcgttaaa gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg
     4741 aacagttggc cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt
     4801 aggggattgg tgaatggccg tactgggatt gcagggggtg cggggaggcg tggggacaac
     4861 aaccatcacc gccgcattag cctggtcatt acaaatgttg ggagaaaatg tcctggtggt
     4921 cgatgcctgc ccggacaact tgttgcgcct gtcatttaat gttgatttta cccaccgtca
     4981 gggctgggcc agagcgatgc tggatggcca ggactggcgt gacgctgggt tgcgctacac
     5041 ctcgcagctc gatttgctgc cttttggtca gttatccatt gaagaacaag aaaatccaca
     5101 gcactggcaa acccggctga gcgatatttg ctccggctta cagcaactaa aagccagcgg
     5161 gcgttaccag tggattttaa tcgacttacc gcgtgatgcc tcgcagataa cccaccagct
     5221 gctgagtttg tgcgagcact cgctggcaat cgtcaatgtg gatgccaact gccatatccg
     5281 actgcatcag caagcgctgc cggatggcgc acatattttg attaatgact tccgtattgg
     5341 cagtcaggtt caggacgata tttaccagct ttggttgcaa agccagcgcc gattactgcc
     5401 gatgctcatt catcgtgatg aagcgatggc tgaatgcctg gcggctaagc aaccagtagg
     5461 tgaatatcgc agtgatgcgc tggcggctga agagatactg acgctggcga actggtgcct
     5521 gttgaactac tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg
     5581 tggttgctta tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt
     5641 cacggtgcgt cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt
     5701 tttattccgc tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat
     5761 acgcatatca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     5821 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     5881 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     5941 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     6001 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     6061 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     6121 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     6181 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     6241 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     6301 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     6361 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     6421 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     6481 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     6541 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     6601 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     6661 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     6721 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     6781 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     6841 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     6901 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     6961 gtggctatac ctccgcgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     7021 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtataa atcttccgtc
     7081 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     7141 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     7201 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     7261 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     7321 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     7381 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     7441 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     7501 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     7561 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     7621 cggtatcggt agaaagcaaa caggtacgcc gatcgaaccg cgtggagatg acgatgcccg
     7681 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     7741 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     7801 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     7861 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     7921 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     7981 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     8041 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     8101 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     8161 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     8221 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     8281 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     8341 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     8401 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     8461 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     8521 gcgatgctca acttcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     8581 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     8641 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     8701 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     8761 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     8821 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     8881 ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc tgccattgtc
     8941 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     9001 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     9061 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     9121 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     9181 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     9241 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     9301 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     9361 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     9421 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     9481 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     9541 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     9601 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     9661 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     9721 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat cccgatgccg
     9781 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     9841 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     9901 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     9961 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
    10021 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
    10081 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
    10141 acccagtcaa ctctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
    10201 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
    10261 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
    10321 atccgcgagt ccggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
    10381 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
    10441 gcgactatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
    10501 cgtcgtcgtc gtcttaaccc ggataacgag taattgaaga tgaatgtgtt gcgtagtgga
    10561 atcgtgacga tgctgttgtt ggctgccttt agtgttcagg cagcctgtac ctggcctgcc
    10621 tgggagcagt ttaaaaagga ttacatcagt caggaagggc gcgtcatcga ccccagcgac
    10681 gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta tgttctttgc cctggcggct
    10741 aacgaccgtg cagctttcga taatattctc gactggacgc agaacaatct cgctcagggt
    10801 tctttaaaag aacgtttgcc cgcctggctg tggggcaaga aagagaacag taagtgggaa
    10861 gtgctggaca gcaattcggc ctccgatggt gatgtctgga tggcctggtc gttgctggag
    10921 gcggggcgtt tgtggaaaga gcagcgttat accgacatcg gcagcgcgtt gctaaaacgt
    10981 atcgcgcggg aggaagtggt gacggtgcct gggctgggtt ccatgttgtt accgggcaaa
    11041 gtgggttttg ctgaggataa cagctggcgt tttaacccca gctacatgcc gccgacgctg
    11101 gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc tgcgcgaaac caatcaacat
    11161 ttattgctgg aaaccgcccc gaaaggcttt tcgccagact gggtgcgcta tgagaaagac
    11221 aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca gctacgacgc tatccgcgtt
    11281 tacatgtggg taggcatgat gcctgacagc gatccgcaaa aagcgcggat gctcaaccgg
    11341 tttaaaccga tggcgacatt cactgagaaa aacggttatc cgccggaaaa agtggatgtg
    11401 gctacgggga aagcgcaggg taaaggaccg gtcggttttt ctgccgccat gctgcctttt
    11461 ttacaaaacc gcgatgcgca ggcagttcag cgccagcgcg tggccgataa ctttcccggc
    11521 agcgatacct attacaacta tgtgctgacc ctgtttggac aaggctggga tcaacaccgt
    11581 ttccgcttct cgacaaaagg tgagttatta cctgactggg gccaggaatg cgcaaattca
    11641 cactaaacat attcacgctt tccctcggtc tggccgtcat gccgatggtc gaggcagcac
    11701 caaccatcag cagaccctgc ggatggacag cggcaacact aatgccgtgc gcgggctggc
    11761 gaatatttac cgccagcagt cgccagaaaa agctgaagcg tttatcgcct cgctctctgc
    11821 cagccagcgg cgcagcattg atgatatcga acgcagcctg caaaacgacc gtctggcaca
    11881 gcaggcagag gtactggaaa accagggcaa atgggcgcag gcggcagcac ttcagcggca
    11941 acgactggcg ctgggccccg gcagcgtatg gattacttac cgactttcgc aggatctctg
    12001 gcaggccgga caacgcagcc aggccgatac gttaatgcgc aatctggcgc agcagaagcc
    12061 gaacaacccg gagcaggttt acgcttacgg gctgtacctc tcaggtcaca accaggacag
    12121 agcggcgctg gcgcatatca acagcctgcc gcgcgcgcag tggaacagca atattcagga
    12181 gctggttaat cgactgcaaa gcgatcaggt gctggaaacc gctaatcgcc tgcgagaaag
    12241 cggcaaagag gctgaagcgg aagcgatgct gcgtcagcaa ccaccttcca cgcgtattga
    12301 cctcacgctg gctgactggg cgtaacaacg acgtgattac accgccgccc gcactgcata
    12361 tcagaatgtc ctgacgcggg agccaactaa cgccgatgcc attcttggtc tgacggaagt
    12421 ggatattgct gccggtgaca aagcggcggc acgtagccag ctggcgaaac tgcctgccac
    12481 cgataacacc tcgctgaaca ctcagcggcg cgtggcgctg gcacaggcgc agcttggaga
    12541 taccgcagcg gcgcagcaga cgtttaataa gttgatcccg caggcaaaat ctcagccacc
    12601 gtcgatggaa agcgcgatgg tactgcgcga cggtgcgaag tttgaagcgc aggcgggcga
    12661 tccaaagcag gcgctggaaa cctacaaaga cgccatggtc gcatccggtg tgactacgac
    12721 gcgtccgcag gataacgaca cctttacccg actgacccgt aacgacgaga aagatgactg
    12781 gctgaaacgc ggcgtgcgca gcgatgcggc ggacctctat cgccagcagg atcttaacgt
    12841 cacccttgag cacgattact ggggttcgag cggcaccggt ggttactccg atctgaaagc
    12901 gcacactacc atgttgcagg tggatgcgcc gtattctgac gggcggatgt tctttcgcag
    12961 tgatttcgtc aatatgaacg tcggcagttt ctccactaat gccgatggca aatgggatga
    13021 caactggggc acctgtacat tacaggactg tagcggcaac cgcagccagt cggattccgg
    13081 tgccagcgtg gcggtcggct ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc
    13141 gatgggcttc aacgtggtgg atgtggtcgg cggcatcagt tacagcgatg atatcgggcc
    13201 gctgggttac accgttaacg cccaccgtcg gcccatctcc agttctttgc tggcctttgg
    13261 tgggcaaaaa gactccccga gcaataccgg gaaaaaatgg ggtggcgtac gtgccgacgg
    13321 tgtggggcta agtctgagct acgataaagg tgaagtaaac ggcgtctggg catcgcttag
    13381 tggcgaccag ttaaccggta aaaatgtcga agataactgg cgcgtgcgct ggatgacggg
    13441 ctattactat aaggtcatta accagaacaa tcgccgcgtc acaatcggcc tgaacaacat
    13501 gatctggcat tacgacaaag atctgagtgg ctactcactc ggtcagggcg gttactacag
    13561 tccgcaggaa tacctgtcgt ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg
    13621 gtcgtgggag ctgggggcgt ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg
    13681 ttatccgctg atgaatctga tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa
    13741 cgatggcggc agcagtcagg gcttcggcta cacggcgcgg gcattacttg aacgacgtgt
    13801 tacttccaac tggtttgttg gtacggcaat tgatatccag caggcgaaag attacgcacc
    13861 cagccatttc ctgctctacg tacgttattc cgccgccgga tggcagggtg acatggattt
    13921 accgccgcag ccgctgatac cttacgccga ctggtaagtt ttcagatagc gcctctctta
    13981 atgccgctgc gatcgggtat actcgggcgg caatctggga tttccggggg gagacaattt
    14041 gcgcgtaagt cgctcgttaa caatcaagca gatggcaatg gtggcagccg ttgtcctggt
    14101 gttcgttttt attttttgca ccgttttgct gttccatctg gtccagcaga atcgctataa
    14161 cacggctacg caactggaaa gcattgctcg ctctgtccgc gaacccttat cttcagctat
    14221 tttgaaaggc gatattcccg aagcggaagc tattcttgcc agcattaaac cggcaggcgt
    14281 ggtcagccgt gccgatgtag tgctgcctaa ccagttccag gcgctgcgta aaagttttat
    14341 tccagagcgt ccggtgccgg taatggttac tcgcctgttt gagctaccgg ttcaaatctc
    14401 gctgggcgtc tactcgctcg aacgtccggc aaatccgcag ccaatagcct atctggtgct
    14461 acaggcggat tccttccgta tgtataagtt cgtgatgagc actctctcaa cgttagtgac
    14521 catttactta cttttgtcgc ttatcctgac cgtcgccatc agctggtgca ttaaccgcct
    14581 gattttgcat ccgttacgca atattgctcg cgaacttaac gccatcccag cccaggagct
    14641 tgttggtcac caactggcat taccgcgtct gcatcaggac gatgaaatcg gtatgttggt
    14701 gcgcagttac aacctcaacc agcaattgct gcagcgccat tatgaagaac agaacgaaaa
    14761 tgcgatgcgc ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg agatgctgga
    14821 gcaggttgtc gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg
    14881 tgatactgcg ggcgtgctga aagaggcgca acgagaaatt ctgctgctga cgctggtgga
    14941 aaaactcaaa tcggtactgt cgccacgtat gatcctcgcg cagattagcg gttatgactt
    15001 tgctgtcatt gccaacggtg tacaggaacc gtggcacgca atcactttgg gtcagcaagt
    15061 actcactatc atgagcgagc gcctgccgat tgaacgtatt caactccgtc cgcactgtag
    15121 cattggcgtg gcgatgttct acggcgatct caccgccgaa cagctttaca gtcgcgctat
    15181 ttctgcggca tttaccgctc gccataaagg caagaatcag attcagttct ttgatccgca
    15241 gcagatggaa gcagcccaga agcggttgac ggaagagagc gatatcctta atgcactgga
    15301 aaatcatcag tttgcaattt ggttacagcc acaggtcgag atgaccagcg gtaaactggt
    15361 cagtgcggaa gtgttactgc gtatccagca accggatggc agttgggacc tgccggatgg
    15421 cttaatcgat cgcattgagt gctgtgggct gatggttacc gtcggtcact gggtgctgga
    15481 agagtcctgt cgattgcttg cagcctggca agagcgcggc attatgctgc ccttgtcggt
    15541 aaacctctct gcgctgcaac tgatgcaccc gaatatggtg gcggatatgc tggaactgtt
    15601 aacccgctat cgcattcagc cgggaacact gattctggaa gtgacagaaa gccgacgtat
    15661 tgacgaccct catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt
    15721 ggcgctggat gatttcggca tgggctacgc agggctgcgt cagctgcagc atatgaaatc
    15781 gttgccaatc gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag
    15841 cagcatgatt gctgcaatta tcatgctggc gcagagcctg aacttacaaa tgattgccga
    15901 aggcgtggag actgaagcac aacgagactg gctggcaaaa gcgggcgttg gtattgccca
    15961 gggcttcctt tttgctcgcc cactccctat tgaaatcttc gaagagagtt acctggaaga
    16021 aaagtagcta ccccaaactg attacaaaac tttaaaaagt gctggtttgt gcgagccagc
    16081 tcaaactttt taaccttttt gtttcaatta tgatccaggt acatttctgt gatgttgtct
    16141 gggtgttatt ttaaggccgc aggtaccccc cataacctta caagacctgt ggttttacta
    16201 aaggacaccc tatgaaaacc tctctgttta aaagccttta ctttcaggtc ctgacagcga
    16261 tagccattgg tattctcctt ggccatttct atcctgaaat aggcgagcaa atgaaaccgc
    16321 ttggcgacgg cttcgttaag ctcattaaga tgatcatcgc tcctgtcatc ttttgtaccg
    16381 tcgtaacggg cattgcgggc atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg
    16441 cactgcttta ctttgaaatt gtcagtacca tcgcgctgat tattggtctt atcatcgtta
    16501 acgtcgtgca gcctggtgcc ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg
    16561 tagcggttta cgccgatcag gcgaaagacc agggcattgt cgccttcatt atggatgtca
    16621 tcccggcgag cgtcattggc gcatttgcca gcggtaacat tctgcaggtg ctgctgtttg
    16681 ccgtactgtt tggttttgcg ctccaccgtc tgggcagcaa aggccaactg atttttaacg
    16741 tgatcgaaag tttctcgcag gtcatcttcg gcatcatcaa tatgatcatg cgtctggcac
    16801 ctattggtgc gttcggggca atggcgttta ccatcggtaa atacggcgtc ggcacactgg
    16861 tgcaactggg gcagctgatt atctgtttct acattacctg tatcctgttt gtggtgctgg
    16921 tattgggttc aatcgctaaa gcgactggtt tcagtatctt caaatttatc cgctacatcc
    16981 gtgaagaact gctgattgta ctggggactt catcttccga gtcggcgctg ccgcgtatgc
    17041 tcgacaagat ggagaaactc ggctgccgta aatcggtggt ggggctggtc atcccgacag
    17101 gctactcgtt taaccttgat ggcacatcga tatacctgac aatggcggcg gtgtttatcg
    17161 cccaggccac taacagtcag atggatatcg tccaccaaat cacgctgtta atcgtgttgc
    17221 tgctttcttc taaaggggcg gcaggggtaa cgggtagtgg ctttatcgtg ctggcggcga
    17281 cgctctctgc ggtgggccat ttgccggtag cgggtctggc gctgatcctc ggtatcgacc
    17341 gctttatgtc agaagctcgt gcgctaacta acctggtcgg taacggcgta gcgaccattg
    17401 tcgttgctaa gtgggtgaaa gaactggacc acaaaaaact ggacgatgtg ctgaataatc
    17461 gtgcgccgga tggcaaaacg cacgaattat cctcttaatc tcaccactta tgcccgcact
    17521 cccttccccg agtgcgggtg tttgcgcata attgacccct gtgcctgaac ttaccacctg
    17581 ggtgagttca ggtttatttc acttcttccg tgacattttc atgttcttgc ggtctaacac
    17641 gaagtgtttt taacgtcatt tagactgacc gacaacgggc agttttttta ttaccaggat
    17701 agttgatcag gggttcacat gcagggcata aaaattcgac ttttagcggg cggtttgctg
    17761 atgatggcca ctgctggcta tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa
    17821 caggggacgc tttccaacgg tttacagtgg caagtgctga ccacccccca gcgtcccagc
    17881 gatcgtgttg aaattcgcct gctggttaat accggttcgc tcgccgaaag tacacaacag
    17941 agcggttaca gtcacgccat ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca
    18001 gcacaggcgc gttcattgtg gcagcagggg atcgacccta aacgcccgat gccgccggta
    18061 attgtctctt atgacaccac gctgtttaat ctgagtttgc ccaataaccg taacgatttg
    18121 ctgaaagaag cgctctctta tctggcaaat gccactggca aattgaccat cacaccagaa
    18181 accatcaacc acgcgctgca aagtcaggac atggtggcaa cctggcctgc cgatactaaa
    18241 gagggctggt ggcgctatcg tctgaaaggg tcaaccttgt taggtcacga tcctgccgat
    18301 ccgctgaaac aacccgttga agcggaaaaa attaaagatt tctatcagaa atggtacacc
    18361 ccggatgcaa tgacgctact ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa
    18421 atcaacaaaa cgtttggcga actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg
    18481 ctttctccgc tgcgtgcgga agcggtgagt attatgactg acgcggtgcg tcaggaccgg
    18541 ttatctatca tgtgggatac gccgtggcag ccgattcgtg aatcagccgc actgctgcgc
    18601 tactggcgtg cggacctggc ccgcgaggcg ctgttctggc atgttcagca agcgttaagc
    18661 gccagtgaca gcaaagacat cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg
    18721 cagtgtgcca tcaacatcga atcaccaaac gacaagctga acagcaacct taatctggtg
    18781 gcgcgtgaac tggcgaaggt tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta
    18841 gtggcgcaaa agaaactgga gctgcagaaa ctgtttgccg cctatgcacg ggctgatacc
    18901 gatattctga tgggccagcg gatgcgttcg ttgcaaaatc aggtcgtcga tatcgcgccg
    18961 gagcagtatc agaaactgcg gcaggatttc cttaacagcc tgactgtgga gatgttaaat
    19021 caggatctgc gtcagcagtt gtcgaatgat atggcgttaa tactgctgca gccgaaaggc
    19081 gagccggaat ttaacatgaa agcgttgcag gcggcctggg atcaaatcat ggccccatct
    19141 accgccgctg cgaccacctc tgtcgccacg gatgacgtac atcctgaagt gacggatatt
    19201 ccacctgcac agtaatgcct gatggtgatg agttagcaca accatcaggt atttatggat
    19261 gagctggata gaggggtaac actttatcca gccttttgca tatgctgcgt ttacgctggc
    19321 atcgcctcac gcgggataat cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc
    19381 ccgcgtttcg ccgcgttttc cgcactgccg cccgtcaaac gtaccgccag ataaccggcg
    19441 ctgaaagagt cgccagctgc ggtggtatcg atcacttttt ctttcggcag tttcaccgcc
    19501 ggaacatcaa ctaacgcttc accagcaatg gacaccaggc aagaatccgc cccgcgtttc
    19561 accaccactt ctttcacgcc cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt
    19621 tgaccccaca gcgcgtcttc gtcgtccagc gtcaggaagg cgatatccgt gcattccagc
    19681 atttgctggt acacctgctg tgtctcttct ttgctggccc acaggcgcgg acgatagtta
    19741 ttgtcgaaaa tcacttttcc gcctttggcg cggcattcgc gcagcaggga aagcagcttt
    19801 tcgcggctgg tcgggcttaa gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa
    19861 ttcgccagct cttcgcaaat cgccgcagac tgctcgctcg ccagccagaa tttggcggcg
    19921 gcttcgttac gccagtagta gaacgtacgt tcgccggtgc tgtcggtttc aatgtagtaa
    19981 aggcccggaa gacggttttc catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc
    20041 caggcgtcca gcatctgctg gctaaaactg tccgttccca gcgccgttac gtaatgaacg
    20101 gttaatgccg caggatcgac ctgacgggcg atatagacgg aagtgttcag ggtatcgccg
    20161 ccgaaaccgc gcttaacgtc cgcgcctttc tcggaaagct caatcatgca ttcgccaatc
    20221 acggcaatct ttttggacat agtcgtgaac ctgatctgta aaaaattagc gttagtttgc
    20281 gctgtggtgg tttgctggtc aaccatatta aaacagtgtt ccattatttt tttgagccag
    20341 aacgtattcc tgaaagattc cgttgtggag tgaggaaatt taccccggcc aggcggtaaa
    20401 gttctgccct tacgcgccga taatctttgt cgagtccggg cagcatcact tttaaacaca
    20461 ggacatcttt gatgataagg caggttatcc agcgaataag caaccctgaa gcaagcatcg
    20521 agagcttgca ggaacggcgt ttttggttgc agtgtgagcg tgcttacacc tggcagccga
    20581 tctatcaaac atgcgggcgg ttaatggccg tggagctatt aacggtggtc acgcatccct
    20641 tgaacccttc gcaacgcctg ccgccggatc gctattttac tgaaatcacc gtcagccatc
    20701 ggatggaggt tgtgaaagag cagattgatt tgctggcgca aaaagccgac ttctttgtag
    20761 agcacggcct gctggcatcg gtcaatattg atggccctac gctcatcgcc ctgcgtcagc
    20821 aaccaaaaat cctgcgccag attgagcgtc ttccctggct gcgtttcgaa ctggtggagc
    20881 atatccgtct gccgaaagat tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc
    20941 tggatgattt tggtaccggg atggcaaatt tctctgcact aagtgaagtg cgttatgact
    21001 acatcaaaat cgcgcgagaa ctgtttgtga tgctgcgtca gtcgccggaa ggccgcacac
    21061 tcttttctca gcttttacat ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg
    21121 tagaaacacc ggaagagtgg cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct
    21181 ggtttctttc acgcccggca ccgatggaaa cgctgaatac ggcggttctg gcgctataag
    21241 ctgcctcatt ttccgccggg ctggactatc tttaggactg gcacaggaag aatatgagga
    21301 agcgaacgat gagcaaggca ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt
    21361 tgattgtcgt ggcgatcatt ttgattgcaa cttttgactg gaatcgactc aaaccgacca
    21421 tcaaccagaa agtctctgcg gagttgaatc gtccgttcgc tatccgtggc aatctgggcg
    21481 tggtgtggga gcggcaaaaa caagaaactg gctggcgcag ctgggtgccg tggcctcatg
    21541 tacatgcaga agacatcatt cttggtaatc caccggatat tcccgaagtc acgatggtgc
    21601 atttgccgcg agtagaggca acgctggcac cgctggcgct gctgaccaaa acggtctggc
    21661 tgccgtggat caagctcgaa aagcccgacg cgcgcctgat tcgcctctct gaaaagaaca
    21721 ataactggac gtttaatctt gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg
    21781 catggtcgtt tcggctggat aatattcttt tcgatcaagg gcggatcgcc attgatgaca
    21841 aagtaagcaa agcggatctg gaaatttttg ttgatccgtt aggcaagccg ctgccattca
    21901 gcgaagttac tggatcgaaa ggtaaagcgg ataaagaaaa ggtgggcgat tacatttttg
    21961 gcttgaaggc gcagggacgt tataacggtg aaccgctcac gggtacggga aaaataggcg
    22021 gtatgctggc gctgcgtggc gaagggacgc cgtttccggt acaggctgat ttccgttcag
    22081 gtaatacccg tgttgctttt gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg
    22141 atttacggct taaattttct ggcgattcac tgggtgatct ctatgaactg acgggcgttc
    22201 tgctgcccga taccccgccg tttgaaacgg atggtcggct ggtagcgaaa atcgacactg
    22261 aaaaatcgtc ggtctttgat tatcgcggtt ttaatgggcg aattggcgat agcgatatcc
    22321 acggttctct ggtctacacc accggaaagc cacgaccaaa actggaaggt gatgtcgagt
    22381 cgcggcaatt gcggctggcg gacctgggac cgttgattgg cgttgattcc gggaaagggg
    22441 cagaaaagtc gaaacggtct gaacagaaga agggcgaaaa aagcgttcag cctgcgggca
    22501 aagtgctgcc ttatgaccgc ttcgaaaccg ataaatggga cgttatggat gccgatgttc
    22561 gcttcaaagg gcggcgcatt gagcatggca gtagcctgcc gattagcgat ctttctactc
    22621 atatcatcct caaaaatgct gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg
    22681 gcagcattgc ggcgaatatt catctggaag gcgataaaaa gccgatgcag gggcgggcag
    22741 atattcaggc tcgtcgactg aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga
    22801 agacgctggg ggaaatgaac ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg
    22861 cgcttttagg caacagtaac ggcaacctga aactgttgat gaatgacggg ctggtgagcc
    22921 gcaacctgat ggagattgtt gggctgaatg tcggcaacta cattgtcggt gcgatatttg
    22981 gtgacgatga ggtgcgggtg aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg
    23041 cgcgcccgca gatttttgct ttcgatactg agaacgcgtt gattaatgtt accggcacgg
    23101 caagttttgc ttcggaacag ctggatttga ctattgatcc ggagagtaaa gggattcgga
    23161 ttatcacact gcgttcgccg ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg
    23221 tgaaagccgg accgctgatt gcccgtggtg ctgttgctgc ggcactggca acgctggtaa
    23281 caccggcggc ggcgttactg gcactgatct caccttccga aggggaggct aatcagtgtc
    23341 ggacgatatt gtcgcagatg aagaagtgat gataattgcc ggatgtctga catccggcaa
    23401 ttaaacagat ttagattttt ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg
    23461 ccttatccgg cctacaaatc cttgtgaatt caatatattg tatggaaatt cagtcctgat
    23521 aagcgtagcg catcaggctt ttcacactta ttacaacgac tgatgtcgcg tctcatgggt
    23581 cagcagcagg gcgattaacg tcaagccagc catcgccgcc agatataacc ccaccgcacc
    23641 taacccgtag ttagcctgca accaggctgc gatatacggc gcaacggaag ccccgagaat
    23701 cgacgatacg ttgtaagaga acgatgctcc ggtgtaacgc acttctgtcg gaaacagctc
    23761 tggtaacagc gcacccatcg gcccgaaggt cagccccatc agacttaacc ccagcagcag
    23821 gaaggcaaaa accagaatcg ggttgccaga accgagcagc gggttaaagg cgaacagcgc
    23881 gaacaggatg atcagcgtgg tgatgatcac catgctttta cggcgaccaa aggcatcagc
    23941 cagtaatccg gcgactggca ccatcacgcc aaaaccaata actgccatca tcagcatcca
    24001 caacacttcg ttacgcggca ggccaagccc aactggcgcg gcggcggtac tgaaggtcat
    24061 agagtagacc gtcatgatgt aaaacagcgt ataggttgcc agcataatga acgtacccag
    24121 tacggttacg cgaacatgtt tggtcagcag cgtacccagc gggatcttca cctgtttttt
    24181 cgctttagcg actttctcaa acaccggcga ctcatgcagc gacacgcgaa catacaggcc
    24241 gataatgacc agcaccgccg agaagataaa tggcacacgc cagccccagc tcataaactg
    24301 ctcatcggtc agtagccagg aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat
    24361 cggtgcgcct agctgtggga aggagccata cagtgcacgt ttgcgcggtg gggcgttttc
    24421 agtcgccagc agcgccgcgc cgccccattc accgcctaag cccagaccct gaccaaatcg
    24481 agccagcgcc agcagcagtg gggcgaaaat accaatcgtg gcatagcccg gcagcagacc
    24541 aatcaccacg gtcgaaatcc ccatcgttag caacgaggcg accagcgtcg ctttacgccc
    24601 aacgcgatcg ccaaaatgac caaaaacggc agagccaatg gggcgcgcga cgaaggcgat
    24661 ggcgaaggtg gcgagcgact gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat
    24721 atgcggaaac acaataacgg ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc
    24781 cgtgccaatg agagaggcga caaggacttt attacgcgag ttgatcggcg tgtattcttg
    24841 ttcgtggtcg agtgttgtgg ctgttgcttg cataatcttt tcttattttt tggctaacga
    24901 atagccatat tacgcacagc aaaagtgata tttcaatgtg taacaaatcg cggaaatggt
    24961 caaaaaccag gcaaaaaggg gataatgttt aggccagtga tttttcttct atgaaatgct
    25021 tcacataatt ttataaatag tggataaaac tggtttcagg ttaaataaaa gttaccaact
    25081 ggatttatat gctggggtta tgagttggag tgaaattgtt tgctcccaaa tagatggctg
    25141 aagaggctct ggggaagagt ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct
    25201 ggcgcatctt atccggccta cggggagcca ggccgaataa aatcgtatgc cggataaggc
    25261 gtttacgccg catccggcct attttaaggc tgcgttttcc ctggcattcg cgggtcgtct
    25321 ttatattcgg cggtggcaat ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag
    25381 aaaaatgcca tcagccccag cacggaacca aatgctgcgc cagaggggga tttcatcagc
    25441 gatggcaggg tgtaggtcat cacgatttta atcacttcaa aaccaatagc agccagaaat
    25501 gttccgcgaa tcagggcttt tttgcgcgga cggtggcgcg gcaaccgcca gaagatccag
    25561 aagaaaagca gatagttggc gaagatggaa atcgccaggc caatcaatcg ccacgtcggt
    25621 ttcagccact caatgctgtt caggtgcagg gcgctaataa tcatttgctg cgccgaaccg
    25681 gcaaccgagg tgatcgaaag cgtcacaatc agcgcaatca acaaaccaat cagtgaaata
    25741 aaatcacgca gatattttac ccagaacttc tcctgatctt gcggcgagcg ttcccagaca
    25801 tcgcgcgact gggcacgaat cgcttcacgc aggttaccca tccagttgat gccggaataa
    25861 agtgccaccg ccaggccgac aagccctaca gtcgtacgct gctgaacggc ggtgttgatg
    25921 gtgtttttca acgtggcggc tagtgtcgga tcgctgatgt tttgcagaat tttgtcgaag
    25981 atatcctgta gcaacatcgg atgggaggcc agcacaaagc cccctgcggc aaacgacacc
    26041 atcaaaatcg ggatcatcga caaaaatgag aaataggtga tagccgcacc aaactggtta
    26101 cccagccgat cgttaaagcg ttctgtcgcg cgaataaggt gcgcaataac cggttggcgt
    26161 tggacttttt cggcagtggt ggtgacggtt tccagcgcct ggctaacttt gccgcctttc
    26221 tcgctattat ccaactgctt aatcggctcg tgctccagat cctgggtggg acgtttgatc
    26281 tcgttttcct gcgtcatccg ctcttttgtc cttctgtcgt ggtcattctt aaaaagtata
    26341 gtcggtcagt ccaggtgctc tttcattacg ccagccagcc actgcataaa caggtttaca
    26401 cgccgggaaa gctcccgacg ctgcgggtaa accagcgaaa gggagagcgg ctcggcacgg
    26461 tagcctggca acacttcaat aagccgcccg gcacgcaggg cttcgcgcac ggcaatgcgc
    26521 gggatctgaa taatccccag accggcaaga ccggcggcga gatagttttc gctactgttt
    26581 accgtcaaca tgccgccaga cttaaaccac tggacgccat taacgctggc aacctcaaaa
    26641 cctaacggat gtacacccag gtgcggtgta taacgcacta ttgcgtgtga agtcagatca
    26701 tcggggcttt gcggataacc aaagcgcgtc agatagtgcg gactggcaca gttgaccatg
    26761 gttagtttgc cgaggggacg ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc
    26821 acgcaatcaa aaccatcatg aagaatatct accggacggt tatggctact cagttccagc
    26881 tcaattcccg gatgctgata gagaaattcc gacagacgcg gcagtaacag gtttttcgcg
    26941 attcctgacg ggatgtcgat gcgtaattta ccgctgatac tggtgacatc ctgttgaaac
    27001 agaccgtcca gttcgctgag attactcaac acatcttttg cccgttgata ataggtcatg
    27061 ccttctggcg tgagtttgac ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga
    27121 tgttccagtg cctgtatctg gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc
    27181 gcgcgggaaa aactttccag ctccgcgact ttgatgaaca actgcattgc gtgaatttta
    27241 tccatttctt tgaccgattg ttgttcacag cgaaacagta acgcgcatat cgcattgttt
    27301 attgtttttt ctaacatcta atgccgggtt tcacaatgtt gtcatgacga atgtggcgat
    27361 ttctcagcag gaaaagggcg gaatagcttt atgagaaagg tgaatcccct taaacagagc
    27421 gtatttttca gtgattccct ttaaagtgaa tagaggacgt attggctatt tgtgcttatt
    27481 tttgtctcat tgccatttgt tattttggct tgtagtcgcg gaatttcatt tttaattgca
    27541 tgcgtactaa ccaccaatat aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca
    27601 tgtaatttca ggcgtttagc tgtaactatt tatatcggta actattttct acgcgtcatc
    27661 tctgtagaat ggccaccgcc aaatggcgaa ttcttatttt ttctcgcggg taataccctt
    27721 ccactactta agtcttttta acgtattcag gtggagattc agttatgcaa atagtcatgt
    27781 ttgacaggca gtcaatattt attcatggaa taaaaatcag tttacagcag cgtattccag
    27841 gagtgagtat tcagggggtc agtcaggcag acgagttatg gcaaaagctg gaaagttacc
    27901 ctgaagcctt agttatgctc gatggcgatc aggatggtga gttttgctac tggttgctgc
    27961 aaaaaaccgt ggtgcagttt cctgaggtta aggtgttaat cacggcgacg gattgcaata
    28021 aacggtggtt acaggaagtt atccatttta atgtgctggc cattgtgcct cgcgattcaa
    28081 ccgtcgagac atttgcgttg gcggtaaata gtgcggcgat ggggatgatg tttctgcctg
    28141 gagactggcg gacgacgccg gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc
    28201 agcgggagat tttaaccatg ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc
    28261 tgaatatcag taccggaacg gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag
    28321 tgaaaaatcg cacccaggcg gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAJTRB010000053        28247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTRB010000053 DAJTRB010000000
VERSION     DAJTRB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.053"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(6..1001)
                     /gene="yjfF"
                     /locus_tag="OO330_002369"
                     /pseudo
     CDS             complement(6..1001)
                     /gene="yjfF"
                     /locus_tag="OO330_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(988..2010)
                     /locus_tag="OO330_002370"
     CDS             complement(988..2010)
                     /locus_tag="OO330_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5883210.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2024..3526)
                     /locus_tag="OO330_002371"
     CDS             complement(2024..3526)
                     /locus_tag="OO330_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5883211.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3603..4550)
                     /gene="ytfQ"
                     /locus_tag="OO330_002372"
                     /pseudo
     CDS             complement(3603..4550)
                     /gene="ytfQ"
                     /locus_tag="OO330_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4860..5390
                     /gene="ppa"
                     /locus_tag="OO330_002373"
     CDS             4860..5390
                     /gene="ppa"
                     /locus_tag="OO330_002373"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5883212.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5769..6110)
                     /locus_tag="OO330_002374"
     CDS             complement(5769..6110)
                     /locus_tag="OO330_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5883213.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6113..9892)
                     /gene="tamB"
                     /locus_tag="OO330_002375"
     CDS             complement(6113..9892)
                     /gene="tamB"
                     /locus_tag="OO330_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5883214.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9889..11622)
                     /gene="tamA"
                     /locus_tag="OO330_002376"
     CDS             complement(9889..11622)
                     /gene="tamA"
                     /locus_tag="OO330_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5883215.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIPRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11828..12457
                     /gene="msrA"
                     /locus_tag="OO330_002377"
     CDS             11828..12457
                     /gene="msrA"
                     /locus_tag="OO330_002377"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5883216.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGVCLPPEA"
     gene            12780..14123
                     /locus_tag="OO330_002378"
     CDS             12780..14123
                     /locus_tag="OO330_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5883217.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14185..14391)
                     /locus_tag="OO330_002379"
     CDS             complement(14185..14391)
                     /locus_tag="OO330_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5883218.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14716..15270
                     /locus_tag="OO330_002380"
     CDS             14716..15270
                     /locus_tag="OO330_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5883219.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15333..16271)
                     /locus_tag="OO330_002381"
     CDS             complement(15333..16271)
                     /locus_tag="OO330_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883220.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16483..17223)
                     /gene="cysQ"
                     /locus_tag="OO330_002382"
     CDS             complement(16483..17223)
                     /gene="cysQ"
                     /locus_tag="OO330_002382"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5883221.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17413..19356
                     /gene="cpdB"
                     /locus_tag="OO330_002383"
     CDS             17413..19356
                     /gene="cpdB"
                     /locus_tag="OO330_002383"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5883222.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19482..19862)
                     /locus_tag="OO330_002384"
     CDS             complement(19482..19862)
                     /locus_tag="OO330_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5883223.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            19951..20811
                     /gene="qorB"
                     /locus_tag="OO330_002385"
     CDS             19951..20811
                     /gene="qorB"
                     /locus_tag="OO330_002385"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5883224.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            20920..21885
                     /locus_tag="OO330_002386"
     CDS             20920..21885
                     /locus_tag="OO330_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5883225.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            21993..22655
                     /gene="ytfE"
                     /locus_tag="OO330_002387"
     CDS             21993..22655
                     /gene="ytfE"
                     /locus_tag="OO330_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5883226.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22700..24112)
                     /gene="cycA"
                     /locus_tag="OO330_002388"
                     /gene_synonym="dagA"
     CDS             complement(22700..24112)
                     /gene="cycA"
                     /locus_tag="OO330_002388"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5883227.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24420..25040)
                     /gene="fklB"
                     /locus_tag="OO330_002389"
     CDS             complement(24420..25040)
                     /gene="fklB"
                     /locus_tag="OO330_002389"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5883228.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25259..25804
                     /locus_tag="OO330_002390"
     CDS             25259..25804
                     /locus_tag="OO330_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5883229.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25788..26435)
                     /locus_tag="OO330_002391"
     CDS             complement(25788..26435)
                     /locus_tag="OO330_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5883230.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26647..27438
                     /locus_tag="OO330_002392"
     CDS             26647..27438
                     /locus_tag="OO330_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5883231.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27448..>28247
                     /locus_tag="OO330_002393"
     CDS             27448..>28247
                     /locus_tag="OO330_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5883232.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6934 a         7516 c         6959 g         6838 t
ORIGIN      
        1 catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg attctcccac
       61 agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc gatggcgatt
      121 ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat cagtccctga
      181 attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa caacgtaccg
      241 ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag tgcatatccg
      301 gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt ggagagcata
      361 taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga cgtcgcgttg
      421 ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa aataccgata
      481 accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc ggggattttc
      541 cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga ctcttccgaa
      601 acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat aaatgccggg
      661 atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc catcaccagc
      721 actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt tgccagaaac
      781 acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga gaggatcaca
      841 aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat attgcagatc
      901 acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc cagcacaaag
      961 acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta ctcctttaat
     1021 cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca ccgctttcac
     1081 cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca tcccctgaat
     1141 aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca gcgatccgcc
     1201 gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt tgttggcatc
     1261 ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata gcccgctcaa
     1321 cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg ccgcccgaat
     1381 gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca acagccagaa
     1441 caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca gcgatccgct
     1501 gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg tgatcagttg
     1561 cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct gaattttgag
     1621 gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca gggcgcttaa
     1681 caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg ctccggcgat
     1741 agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca gcgtcatacc
     1801 aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc taccgaacaa
     1861 acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat cgaccagcag
     1921 caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac gcctttttgg
     1981 cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc gcaatggcgt
     2041 tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt ttgcgatcgc
     2101 gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag gagatcacca
     2161 gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg gcgtgggcac
     2221 caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg gtcagtagcc
     2281 aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc ggttgttcag
     2341 ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct tgctgctctt
     2401 tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg atattttccc
     2461 gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag aatccgatgc
     2521 ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg atcaacgccg
     2581 tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta cgtccggatc
     2641 ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca aacggtgcta
     2701 tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc aacaatgttc
     2761 gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt tttaccagtt
     2821 cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg cgtaagacgg
     2881 tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag atcaggctga
     2941 cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc tgggtgtcga
     3001 ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata gcccggcaaa
     3061 tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc acgtcgaggg
     3121 agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta cggcgtagaa
     3181 ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac atattgggta
     3241 gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg gcggtatttt
     3301 tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg tatacaccag
     3361 ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc gccatgattt
     3421 cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg aaaaatttac
     3481 ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt tcccccggtg
     3541 taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag gcatagcaac
     3601 cctcaatacc ccatattttt cttcttctct aactcttctt ttgcggtatc aggcaggtaa
     3661 agggtggatt tggttaacgt cagcttttca ggcatggtgc cgtctttttt gtatttctcc
     3721 agcgcatcga aagcgggtcc cgccatgttc ggcgtcagtt caacactggc gttcgcttcg
     3781 ccatccatca tcgctttgta gatgtccggt acaccgtcaa tggaacccgt gaggatatct
     3841 ttgcccggtt tcaggcccgc ttctttaatt gcctgaattg caccaatcac catgtcgtcg
     3901 ttatgggcgt aaaccatgca gatgtttttg ccgttgtttt ccgctttgat aaagctctcc
     3961 atgacttctt tgcctttact gcgggtgaag tcacctgact gcgagcggat aattttgata
     4021 tttggcgcat tcttaatggc ttcggcaaag cctttcttac ggtcaatggc gacgctggcc
     4081 ccaacggtgc cctgcagctc caccacgttg catggtttgc cattcacttc ttttaccagc
     4141 cagtcaccga tcaacttacc ttcgaggatg ttgtcggcag taacggtggt catatagaga
     4201 gatttgtctt tcacatcgat ggaacggtcg agcaagaaga ccgggatttc ggcatctttc
     4261 gcctctttta ataccggttc ccaacccgtc gcaaccactg gagcgataaa gatcgcatcc
     4321 accccttatg caacgaagga acgtaccgct ttaatctggt tttcctgctt ttgctgacca
     4381 tcggcaattt tcaacgtaat tccgcgtttt tcggcctcac ttttcgccac attggtttct
     4441 gcggcgcgcc agccggattc cgatccgacc tgcgaaaatc ctacggttaa tggagcggcc
     4501 aacgccatag acgacatggc tgccgagact acaagtaagc gtttccacat agggttgtcc
     4561 tcgtcggggt aattattatt ggtgaaaaga tgttcgcgaa aaaactatag acaattcgtt
     4621 atgtaacgga ttgcgttaca tcacacttca aaaaagtaaa taaaacgtta atcacaagtt
     4681 tgtaatcgct ttcatctcac tatgaaaaat gcggctacgg ttatggattt tcctgctctg
     4741 tataccgtct taaaactggc gaaaaaggaa aatgaagacg aaaacaagca aagacattcg
     4801 gcgcgagttg gctataatac ttggcacttg tttgccacat atttttaaag gaaacagaca
     4861 tgagcttact caacgtccct gcgggtaaag atctgccgga agacatctac gttgttattg
     4921 agatcccggc taacgcagat ccgatcaaat acgaaatcga caaagagagc ggcgcactgt
     4981 tcgttgaccg cttcatgtcc accgcgatgt tctatccgtg caactacggt tacatcaacc
     5041 acaccctgtc tctggacggt gacccggttg acgtactggt cccgactccg tacccgctgc
     5101 agcctggttc tgtgatccgt tgccgtccgg ttgccgttct gaaaatgacc gacgaagccg
     5161 gtgaagatgc gaaactggtt gcggttccgc acagcaagct gagcaaagaa tacgatcaca
     5221 ttaaagacgt aaacgatctg cctgaactgc tgaaagcgca aatcgctcac ttcttcgagc
     5281 actacaaaga cctcgaaaaa ggcaagtggg tgaaagttga aggttgggaa aacgcagaag
     5341 ccgctaaagc tgaaatcgtt gcttccttcg agcgcgcaaa gaataaataa gttcttctag
     5401 cgcaataacc ctgaacgccg ggcttcggtt agtaagggtt tttttatgcc cgcgataaat
     5461 aaactctcta ttccaccatc attattctca gcggttgcaa ggcttgaacg gtaagaacaa
     5521 gcaaacccga ccaccatttt gctgttcata gccacttgct ggaagttagc cgacctcact
     5581 catactcacc gttcaaagcg tgcccccgtt ttacccctgc acatttgtga acgccagcag
     5641 agttttttat gcgattgcag atatggaaag cggctcggag agaaaagcaa aagatggaaa
     5701 agttacaaaa aaaatcgcga gatacatcgc ataaaacaac gccacccgaa ggaggcgtgc
     5761 ttttattatt acttatccct gtctaaccag tcgccgcttt caattagctt taatccatcg
     5821 acgggtcgtt gataaacgta catccatgca ctcccatacg gcgtctgaat caactggcgc
     5881 gcgtattcac cgcccctggt gcgcaaggca tcaagttcgg ccagcgtggc gttgtcaata
     5941 cgataaactt caccgtgtac cgttccgttc cccggaactg cgcctggata gtggcccagg
     6001 ctatacaact ggtagttatc gatactgaaa tcgcccagta actgggcatt ggtcatccag
     6061 tgactgttgc cttgtttgtg gcgtaaactg ccgtagacaa atattcgcat tgctaaaact
     6121 cgaactgata gagcaaatcc agtgcctggt ctacaccaga cacggcttcc agatatagct
     6181 taggcatcag gcgataacgt aacgtgagtg ttgctataga gtcaaatata cccacgccgt
     6241 atttcacttg cagacctggc aatacatagc cgctgaccac tacctgggag gagtcgccta
     6301 ctcccagggt gtcgagcgct aaattgctta cgccaaacgt ctcgccgatt ttacccacaa
     6361 tctggccact ttgcgcaacc cccagaccaa tcagcatcga ggtcattgcc gcactgtcgc
     6421 tctgatcgct ctccagccct tgtccacgta gcaaataaga caatgcagct tgttgcgaca
     6481 tcgccgggtc agagaagatc tccgctttcg gttcgtccgc cagaccagtg acgcgaactc
     6541 cggcgattac atcgtcttct gtagcatccg ggttacgaat agcttcaata ttaagatacg
     6601 gttgatccgg cggaccagag aacagtaact cacccttacg cacaatcaga tcctgaccat
     6661 aggcatggaa gcgcccttca gggatgttga tctgcccgtt caggcccagc ccttgtttgt
     6721 cctgtacgac gttaagatcg cccgtcagcc gcgctttcag gccaaaggcg tcaatgcgca
     6781 cattgttgcc aacgtggaca atcaggttac tgttaatcgg aatcgacgcc gttttcggct
     6841 cttccggttg caggttatcg ttaagcatca ccacatcgct ggagacgcct actgcgcttt
     6901 ccggcagatc gtgcaccacg atgcgcgccc acgggacatc cacgcgacca tcgagggtaa
     6961 acaggtttgg tgtagcctcg aatacaacat ctggcgatac atccattcgt accatcggcg
     7021 gcacggtgat ccgcacttta ctgcctttcg ccgttactcg tgcccgccag ttttcaattt
     7081 ggctccagtc ggcattaccg ttcaggtaga tttcaccctg ctgggtccgt actgtaccgg
     7141 caagcgtcga gcgcataccg ttaaagttga ccgcaagctg gctcggctgc atatcaaacg
     7201 gcataaagtt gccgtcgata tccacacccg taacctgaag ctgaccaaac aactgcgggc
     7261 tttgcacatc accacccaga cgcaagttgg cactcaccat ccccgctgct ttttccccac
     7321 gggtaaagat ggggtttatc atcgcaaggt tgaagttacg gatattgacg ttgccaccaa
     7381 gattacggcg gccttgcgga tcggtcacct gcacctgtcc atcaaactgg ccgttattgg
     7441 tcaggcggat ggtccagccc aattcggcac ggttgttacg caattccgcc gtcagattca
     7501 gtgtctgaaa cgccaccggc agcgccgcat cgttgacggt ttgcgttacc tgcacgttac
     7561 gccccgaaag ggtgatactg ccctgcggca gcccctcttt cgtggtgtcc caggcaacat
     7621 ccgctttacc cgtgaagata ccgctggcct gagtggtttc tggcataaac ggtttcagca
     7681 tggcgaggtc gaagcggttg agattcacca ccgcacgccc ttcggccccc gcatcgatag
     7741 tttgcggcac gcacagttcc gcattcgggt taagccagca gtgtggcccg atgctgattt
     7801 tttgctcctt attgcggtaa tccagcgcaa tatcgcgggt cagcgaccac gggccgaccg
     7861 gcgtctggaa gcgggtattg ctaagagttc ccttccagcg ttcttctttg cgatcaaaac
     7921 ttcctgccag attaagctgc ccggagacag gctcgccctg aatccgcaac tgtagctcgt
     7981 gctgcttttc gctgcctttg gcattcaggg tgacgaggtt gatatttaca tccggctgcg
     8041 aaatttgctc aacgcgtacg tcgagtttcc cggcgatctg atcggtggat ttaatgtcgc
     8101 cttccacgcg aacctgcgcc acggaaagtt cctgccagcg caggccgcgc gcggtgatat
     8161 ctgccagtag ttgtggcgct tccaccgtgc cgcgtacttt caccagccct ttcgctgtac
     8221 cgccaagccc cggcagcgcg ttatccagcc ccggcgcgtt gatggtggca tcaagattga
     8281 gatcttttac ccccagctcg cctttcactt cggcactgtt tggcccgagt tccagatgaa
     8341 gcccagggat catccactgc atataactgt tgcctttcag cgtgccgtca acgttcactt
     8401 tgttctgttt gacgttaccg gtcagcttca gttctggcac ctccatctgc caggtgccgc
     8461 cgtacaggct accgcgggtt ttaatcaagc cattgagttt cgacggccac tccgggaact
     8521 ctttggcggt gttaatgcca ttaagcgtta gctcaccgcg ccaactaatg gcctgctgcc
     8581 agtcgagcaa cgccttgagt tcagttttcc cttccagcgc cgcgacggtg agtttgtcga
     8641 gattgacctg ctgttcatta cctttggcat caagggtaat ggtggctggc gggatctcct
     8701 gtcccttcac tgccgtacgc atagagagcg tgtaatcggt cattttgccg gtaagtttca
     8761 gtttcagatc atccgcctga tactgcttct caccagtgaa cggccagtaa agctgtttgc
     8821 tgttcacttc cacgttgagt ggcaatccgg cttccgccag tcgcgtctgg gcgcgtaaat
     8881 ccatatccac cggaccggaa aggttaacgc ctatctccag ctgttcgcgc agcgcgccgc
     8941 ccactttcag cttcaccttt tcacctttca acggctccac gttcagtgtg ctgttgaggg
     9001 tgatatccac cggccagttg tctgacagct gcgccgtgcc gctggcgttg acgatcccct
     9061 ggttggaatc gatatccagg gcgtccagtt tagtattgcc gtcaatgctg ctcactttca
     9121 gcagcatggt gcgcacggtg atgtccgtgt cgcccgtcac gcgcagctgc tcgcccttaa
     9181 actcttcaat gttcaggtta agcggcaaat gcacgtcggt catttccggc aataccgggc
     9241 gagaaaagag atctttcagc gtttcgccga gcggcttttc ctccggctgc ggattttcaa
     9301 ttttcggttc gaccacttct tcctgcgcca cttccaccac tttcggcaga gcaatcagca
     9361 ggcctttcag cgacgtcggt ttcagggtca gggttttctc ctgccagttc aggccggagg
     9421 tgaagtccat caccgacacc gtggtgtcat caatcttgat gttgacgttg tccagcgcca
     9481 cccgtgtcag ggtgatggga tacggcgtgg agagatccag cggaccgcta tcttcctctt
     9541 cttcaacctg ttcagaagga ggcatttttt tactgtcgat gttgacctga atgtctttca
     9601 gcgccaagtc attaatacaa acgctactgt tccacaggca ctcaagaccg acccccagat
     9661 gcagattacc cgcttttacc gccacgccta gctgctcata acgaacgtca gacaaggtga
     9721 gatcgcgcca gccgccggtg accttgccaa tatccagtcc tggcacccag cgatccgccg
     9781 ctttaaatac aagatgcagg ccgctggtgg tgcccaccag aaacgccacc gatcccagca
     9841 acagtaagat aacgataacc acgccgaggc tgattttttt ccataaactc ataattctgg
     9901 ccccagaccg atgtaaaact gtaacccgtg ttcgtcttta tccgcgaccg gtacggcaaa
     9961 atcgagtttg attggcccga ccggcgattc ccagcgtacg ccgaccccgg taccggtttt
    10021 aaagtcgctg cggggaatat cgcttaccgc ttcgccacta tcgacaaaca ccgcgcccca
    10081 ccattttccg gtcacgttgt actggtattc cagcgatccg gttatcaact tcgaggcccc
    10141 tttcaggtca ccgttggcgt atttcggggc gatagatttg tatttgtagc cgcgaatact
    10201 gcgatcgccc ccgacgaaga aacgcagatc cggcggtact ttgtcgaaat caccggtttc
    10261 aatccagccc agcgtgccgc gtgtcacaaa gcgatggcga tcgtacagtg tgcggatcca
    10321 gacgtgctgc gcctggaaaa cggagaaatc gacatctgaa ccccaggccg tgttggagta
    10381 gtcgatagag tagcgttgcg agtctcccca ggttggcatc aggccaccac gagaacgcgt
    10441 gcggctaatc atcaccccag gataaaacag catcgtggtg ttggtaattt caccctgggt
    10501 aaagtggtcg aggctccagc gcaggttaat ggcacgctgc cagccgctgg agagatccca
    10561 gtagcgagaa gccaccagcg tagtggagtc agattcggta tcgttcaggt cagtgcgctt
    10621 aaaaccgccc tgcaccaaat aatattgttc cagtggattc ttcagcagcg gcattttata
    10681 gctgaagtcg agggtctgtt ccggcgcgga aatactggta ctggtggtca aactgtgacc
    10741 gtatgagttc atccacggct ttttccacgt cgctttcacg cgcggtccca cgtccgtaga
    10801 gtaaccgacc ccggtttcga tggtgttttc tgttcgcggc gaaaccacgc ccgtcaatgg
    10861 taatactttc gtttcgcgcg ctttatcaaa ttgtggagcc accaccaccg agttaaacca
    10921 gccggtagca gaaagtcggc ggttcagttc cgccagatct ttcgattcat actcatcgcc
    10981 ctctttaaac ggcaccagat tttgcaggta ttcatcgcgg atttgtgatc cttcaaaggt
    11041 cacatgccca aagcggtaac gttcgccact gttataatca atatcccaga aggctttatg
    11101 caggccgagc gcaatgccca gctgcgcttt ggtaaattcg ctatcgaaat aacctttacg
    11161 caacgcaata ctggttaagg actttttgaa attttcataa tcgccctggt tcagtaccgt
    11221 accaatagcc gggcgagtat cgagcaattt caaatagtct ttatcggtcc gcgcgccgcc
    11281 gcgcaatacc acatcggtgc cgccaattaa caccggcaca cctggcgtga ctttggcgat
    11341 caatacctgc cgccctttct ttggcggtgg acggagatca aattcaatgg tcggctggta
    11401 ataacccagc gctttcagac cttcacggat ggcatcatcg acgcgtgcgc gaaagcgacg
    11461 gtctggcgtc acttcatcac tttcaatcgt agaaagctgc gcacgaacgt tcttttccag
    11521 ctgtcccgat aacccctcga cctgtagacg gacgttcgcg gcgacggcag atccgcttaa
    11581 gcagagtaag cttacacagc ataactgtcg gatatagcgc acattttctc ctgaatatcc
    11641 ttttttcctg cccctggaac gccgttaaac ggcttaacaa aaatccagta atatggatta
    11701 aaaagcagac taaaccccaa atatttctta tgttttactt tagacctatt cacggtggtt
    11761 attgtgtgca aatacgcctc ttgttacaac cttaacccca atgaccgatt ttcgggagag
    11821 cgacaccatg agtttatttg ataaaaagca tctggtttcc cccgccgatg ccctgcctgg
    11881 acgtaacacc ccgatgcccg tagccacgct gcatgcggtc aacggtcact caatgaccaa
    11941 tgtacctgac ggaatggaga ttgccatttt tgcgatgggt tgtttctggg gtgtggagcg
    12001 tctgttctgg cagttacccg gcgtttacag caccgccgca ggctataccg gcggctatac
    12061 gccaaatccg acttatcggg aagtgtgctc cggtgatacg ggtcatgccg aagcggtacg
    12121 cattgtttac gatccttccg tcatcagcta tgagcagttg ctacaggtat tttgggagaa
    12181 tcacgatccc gcccagggaa tgcgtcaggg caatgaccac ggcacgcagt atcgttcagc
    12241 gatttatccg ctgaccccag aacaggatgc cgcagctcgc gccagtctgg aacgttttca
    12301 ggcggcgatg cttgccgccg atgacgatcg tcacatcacc acggaaatcg ctaacgccac
    12361 accgttttat tatgccgaag atgaccacca gcaatatctg cataaaaacc catatggtta
    12421 ctgtggaatt ggcgtctgtc tgccgccgga agcatagcgt tacgggtaca aatgtagttt
    12481 gttgataaag tgctctttgt ctatgcctga tgcggcttaa acgacttacc aggcctacaa
    12541 aatcgcccaa attcaatata ttgcaaggac tgcgtaggcc tgatcggcat agcgcatcag
    12601 gcagtattgc atttatcagc agtctgaatt ttaaccctct ggcgacttta cagtacctta
    12661 cgctatacta gccactgaaa atgccggttc actttcttcg aatcggcttt caatgtgtat
    12721 ttcacacaaa ttaatcaact ttcccttccg agggtctggc ctgaaaggtc ggataagata
    12781 tgttaaacag tattttagtc atactctgct tgatcgctgt aagtgcgttc ttctcgatgt
    12841 ccgagatctc gcttgccgcc tcacgcaaaa tcaaacttaa actgctggct gatgaaggca
    12901 atataaatgc ccaacgcgtt ctgaatatgc aggaaaatcc cggcatgttc tttaccgtgg
    12961 tccaaatcgg tctgaacgca gtggcgattc tcggcggtat cgtcggtgat gcggcatttt
    13021 ctccagcttt tcacagcctg ttctcccgct atatgtcggc agagctctct gagcaactga
    13081 gctttattct ctctttctcg ttagtgactg gcatgtttat cctgtttgcg gatttaaccc
    13141 cgaaacgcat cggtatgatt gcgccagaag cggtggcttt gcgtatcatc aacccgatgc
    13201 gcttctgcct gtacgtttgc accccgctgg tgtggttctt caacggcctg gcgaacatga
    13261 tcttccgtat tttcaaactg ccaatggtac gtaaagatga catcacttct gatgacatct
    13321 acgcggtagt ggaagccggt gcgctggcgg gcgtgttacg taaacaggaa cacgagctga
    13381 ttgaaaacgt ctttgagctg gaatcccgta ccgttccgtc ttcaatgaca ccgcgtgaaa
    13441 acgtgatttg gtttgatctc cacgaagatg agcaaagcct gaagaataag gtggcggaac
    13501 atccgcactc taagttcctc gtctgtaatg aagatattga ccacatcatc ggttatgtcg
    13561 attctaaaga cctgctgaac cgcgtgctgg ctaaccaaag cctggcactg aacagcggcg
    13621 tacaaattcg caacacgctg attgtgccgg atacgttaac cctttcagag gcgttggaaa
    13681 gttttaaaac cgcaggtgaa gacttcgcgg tgatcatgaa cgagtacgcg ctggtggtgg
    13741 ggatcatcac cctcaacgac gtgatgacca cgctgatggg cgatctggtc ggtcaggggc
    13801 tggaagagca gattgtcgcc cgtgatgaga actcatggct gattgacggc ggcaccccaa
    13861 ttgacgacgt catgcgcgtg ctggatattg acgagttccc gcagtcgggc aactacgaaa
    13921 ccatcggcgg ctttatgatg tttatgctgc gtaagatccc gaaacgcacc gattcggtga
    13981 aattcgccgg ctacaaattt gaagtggtgg atatcgataa ctaccgcatc gaccagctgc
    14041 tggtgacccg gatcgacagc aaggccaccg ccctttcgcc aaaactgcct gacgctaaag
    14101 ataaagaaga aagcgtcgcg taacccccag aaacatcaac ggctcctgaa tcaggagccg
    14161 tcttattact gcatagcact ttggttaagc catctctgtt tgcagacgca taacctgacg
    14221 gttaacttcg gacatcactg acaaatgcag cttatccttc acttttggga taagaatctt
    14281 acccttatca aattcaaaag cgccaatgtc cttgatgtat aaccgtccac ggaacaggat
    14341 ctttacgtac ttcgccacct gaagtgggtt gtaccgttgg aaaattttca ttgttatctc
    14401 ctgctgagta ttacgcctgt gcggtgccac aatcggccca actattatga ggcgcaaatt
    14461 ttaatgctta gtgactatag actatccggg caatgtttcc accgcgtata acttttttta
    14521 cctaaaggtt acaattattc agaattatct ttttaccgaa gcgcggtctt cagtataagc
    14581 attcattttt catatgattt gtgcgcttga ccgcaaactg gcatcacact tgcgggaaat
    14641 tcgataaata gcacatatga ttaaaactca gacccaagtg gtcggatcac ctgcatatca
    14701 taagaaggaa acaccatgac cctacgcaag attctggcac tcacctgcct gctgttgccg
    14761 atgatggctt ccgcacatca gttcgaaatc ggtcagcgag tgccgccgat tggcattacc
    14821 gatcggggcg agttggtgct tgataaagat cagtttagct acaaaacctg gaacagcgcg
    14881 cagttagtgg gaaaagtgcg agtactgcaa catattgctg gtcgcacctc tgcaaaagag
    14941 aaaaacgcga cgctgattga agcgattaaa tcagcgaagt taccgcacga tcgttaccag
    15001 accaccacca ttgttaacac cgacgacgcg attccgggtt ccggcatgtt tgtgcgcagc
    15061 agtctggaga gtaataaaaa gctttatccc tggtcgcagt ttattgtcga tagcaatggc
    15121 gtcgcgcgcg gtgcctggca gctggatgaa gaaagttccg ctgtagtggt gctggataaa
    15181 gacggtcgcg tgcaatgggc caaagacggg gcgctcacac cggaagaggt gcagcaagtg
    15241 atggacctgc tgcaaaagtt gctcaaataa tggcaataac ataaattctg gaaaaataat
    15301 tttcccctat cgttatctat gggatagggg aattaagtct ctgagttctt acaccgaact
    15361 aatactggta acgctagttg ctctttttta aacgcctcaa tggtcgattt atatttctcg
    15421 caaatctttt ctttggtaat attactatct gcggccagat tcgcacttac tatcgcatcc
    15481 acttgttcgg ctaattcgac ttgttggcaa cctgcattaa ccagaaaatt ataaataaga
    15541 gagtaatctt catcctccaa aacaacagag gaaccattac gaaaacgttc atcccctcca
    15601 tagattttaa gcgcaaagcc caaagattct atgactatgg caaaattatc atcaattatt
    15661 ttttcgtaaa tataccatcc atcgcattct ttaattccac cgcctggtat ttggagttca
    15721 aatttacccc gttttaattt gcttactaca tcctccaata aggcataacc gaaagatacg
    15781 tcaccaaacg cctggcgaat ataaccttta atcctttttg gtatgtcaga ctgttttttc
    15841 tgcaaaatat cacaggcata caatcttctg ttttgttcta acggaaactc gcggtttttg
    15901 aaatccatga atgagttata ctcatttatt gttatgggaa tttcttcgta ttcaaaccca
    15961 ctgcctcgca atgttattgt atattcagca ccgttatctt taaggctgaa actgataaga
    16021 aaattatcca tggcattaac agtttcattt tttgatgaaa gcacttgata actattttta
    16081 tcaatctgtg ttgcgcaacg aaaggactcc cgcaaagtta acagactatc ataattcgaa
    16141 acccaatctt ttttcttaaa tgcatgctga atattattaa aaaaatcgtt tgaatgtgtt
    16201 tttgtatgcg atgactttac aaataactcc accgcaccaa taaaattatt gtgtctgatc
    16261 ctgggtaaca tatggctacc ttattttatt tggttatttt atgtcaccat taaatatctc
    16321 attatagaag taaactatca aaaaacgctc attattaaaa tgtaacttac tagcaattga
    16381 atttttgcac tttaaataca atctcttttt atttataact ttttaggttg ggataaatca
    16441 ggatggcaga atcaggaaat acactgtttc tgccatctga atttagtaaa tggacactct
    16501 gaaccctgga ttcaggaacg actcgcgcgg agtgtaatcc agcggtttac cctgccagtc
    16561 gtgaacgtgc gctccggcag ctgcagccac agcatgtccg gcggcggtgt cccaaatatt
    16621 cgttggtccg aagcgcgggt acagctgcgc ctgtccttcc gccaccaggc agaatttcag
    16681 cgaagagccg atggacgtgg tctgatgttc gccaagctgt tgcagatact ctttcagctc
    16741 cgcatctgca tgggaacggc tgataaccac cagcggcggg cgcgcatcgc ggacctgaat
    16801 cagcttgcgc acaccgcact cttctttcca ggccttgcct tctgccgcgc tgtacattac
    16861 gttcattacc ggcgcataca ccacacctaa aatcggtttg ccatggtcaa tgagcgcaat
    16921 gttaacggtg aattcgccat tacgtttaat aaactcttta gtaccatcca gcgggtctac
    16981 cagccagtaa cgctgccagt gctgacggac ttcccaaccg ggaggatctt cttcagaaag
    17041 gaccggaata tccggtgcca gcgtacgtaa accgtccatg ataacggtgt gagcggcaat
    17101 atccgctgcc gttaccggag aattgtccgc tttgctgacg acgtccatcg gtttcgtccc
    17161 gtcgtagacc tgcataatgg catcgcctgc attccgtgca agctggcata cttgatctaa
    17221 catttctcca cctcgtctct gtgagcggtg ttaacttatt gttttactta taccctatcg
    17281 ttaatgaatg cgccaactgt gatagtgtca tcattttcaa agcgtaaaat tgtggcattc
    17341 ttcactgttc tataagtaag gcgtttattc tcccttttct ttcgtattcc cgatgataaa
    17401 aggatgtccc tgatgattaa gtttagcgca acgctcctgg ccacgctgat tgccgccagt
    17461 gtgaatgcag cgacggtcga tctgcgtatc atggaaacca ctgatctgca tagcaacatg
    17521 atggatttcg attattacaa agacaccgcc acggaaaaat tcggactggt acgtacggca
    17581 agcctgatta acgatgcccg caatgaagtg aaaaacagcg tactggtcga taacggcgat
    17641 ttgattcagg ggagtccgct ggccgattac atgtcggcga aaggattaaa agcaggtgat
    17701 gttcatccgg tttataaggc gctgaatacg ctggattata cggtcggtac actcggcaat
    17761 catgaattta actacggtct ggattacctg aaaaatgcgc tggcgggagc gaaattccct
    17821 tatgtaaatg ccaacgtcat tgacgccaga accaaacagc caatgtttac accgtattta
    17881 attaaagata ccgaagtggt cgataaagac ggaaaaaaac agacgctgaa gattggctat
    17941 attggcgtcg tgccgccgca aatcatgggc tgggataaag ctaatttatc cggaaaagtg
    18001 acggtgaatg atattaccga aaccgtgcgc aaatacgtgc ctgaaatgcg cgagaaaggt
    18061 gccgatgtca ttgtcgttct ggcgcattcc gggctgtctg ccgatccgta taaagtaatg
    18121 gcggaaaact cagtttatta cctcagtgaa attccgggcg ttaacgccat tatgtttggc
    18181 catgctcacg ccgttttccc gggtaaagat tttgctgata tcgaaggggc tgatatcacc
    18241 aaaggcacgc tgaatggtgt tccggcggta atgccgggca tgtggggcga tcatcttggg
    18301 gtggtcgact tacaactcag taatgacagc ggtaaatggc aggtgacgca ggcgaaagcg
    18361 gaagcacggc cgatttacga catcgccaat aaaaaatccc tcgcggcgga agacagcaag
    18421 ctggtagaaa cactcaaagc cgatcacgat gccacacgcc agttcgtcag caagccaatc
    18481 ggtaaatctg ccgacaatat gtatagctat ctggcgctgg tgcaggacga tccgaccgtg
    18541 caagtcgtga acaacgcgca aaaagcgtat gtcgaacatt acattcaggg cgatccggat
    18601 ctggcaaaac tgccggtgct ttcagctgcc gcaccgttta aagttggtgg tcgcaaaaat
    18661 gacccggcaa gctatgtgga ggtggaaaaa ggccagttga ccttccgtaa tgccgccgat
    18721 ctttatctct accccaatac gctgattgta gtgaaagcca gcggtaaaga ggtgaaagag
    18781 tggctggagt gctccgccgg acagtttaac cagattgctc ctaacagcac gaaaccacag
    18841 tcactcatca actgggatgg tttccgcact tataactttg atgttattga tggtgtgaat
    18901 tatcagattg atgttaccca acccgcccgc tatgacggcg agtgccagat gattaatgcc
    18961 aatgcagagc ggattaagaa cctgaccttt aatggcaagc cgattgatcc gaacgctatg
    19021 ttcctggttg ccaccaataa ctatcgcgct tacggcggca aatttgccgg tacgggcgac
    19081 agccatatcg cttttgcttc accggatgag aaccgctcgg tgctggcagc gtggattgct
    19141 gatgagtcga aacgtgcggg ggaaattcat ccggcggcag ataacaactg gcgtttagca
    19201 ccgatagctg ccgataagaa actggatatc cgtttcgaaa cttccccgtc agataaagcc
    19261 gcagcgttta ttaaagagaa agggcagtat ccgatgaata aagtcgcgac cgatgatatc
    19321 gggtttgcga tttatcaggt ggatttgagt aagtaaaaca cttctttttc ggcctataaa
    19381 tcaaccgcat ccggcattta ttggcggatg cgatgctgcc gtatcttatc caccctacaa
    19441 gccatgaacc gtagaccgga taagcgcagc gcatccggca gttatgccgc acgttcatcc
    19501 cgcaccgcca acacctcggg caaattcaac tcaatccagt ccgccagtgc agcaaccttt
    19561 tcgctcacct gctcgcccag cggcgtgagg ctatattcca catgcggcgg caccaccgga
    19621 tacgcgatac ggttaagaaa accatcctgt tctaacgcct gtaacgactg cgcaagcatc
    19681 ttttcactca ccccaccgat tttgcgccgc aggtcgctaa agcgatgagt accttcgcgt
    19741 agcgccacca gaatcaacac cccccaacgg ctggtgacgt gtttcaacac ctcgcgcgac
    19801 gggcactgtt ccgcaaagag attaccctct ttcagttgtt gtgacaggct aacctgactc
    19861 atttcatact tacctttttg cacgtactta ctaaaagtaa gtttaggtgt tagcgtattt
    19921 aaacacaaga caaaacgatg gagacttccc atgatcgcta ttactggtgc cactggccaa
    19981 cttggtcact atattattga atccttgatg aaaacggttc ctgccagcca aatagtggct
    20041 atcgttcgta atccggcaaa agcccaggcc ctggcagcac aaggcattac cgtgcgtcag
    20101 gctgactaca gcgatgaagc cgcactgaca tctgcacttc agggagtgga aaaactactg
    20161 ctgatctctt ccagcgaagt gggtcaacgt gccccgcagc atcgtaatgt tattaatgcc
    20221 gcaaaggcgg ctggcgtgaa atttatcgct tataccagcc tgctacatgc agatacctcc
    20281 ccgctcggcc tcgccgatga gcacatcgag acggagaaaa tgctggctga ttctggcatc
    20341 gtttacaccc tgctgcgcaa cggctggtac agcgaaaact acctcgccag cgccccggca
    20401 gcactggaac acggcgtatt tatcggtgcg gcgggcgatg gcaaaatcgc ctcagcaacg
    20461 cgggcagatt atgcgacagc tgcggcacgc gtgattagcg aagccggtca cgaaggcaag
    20521 gtttacgaac tggcgggcga tagtgcctgg acgttgacac agttagcggc agagctgacc
    20581 aaacagagcg gcaaacaggt tacctatcaa aatctgagcg aagccgattt cgccgcggca
    20641 ctgaaaagcg tcggactgcc cgacggactg gcggatatgc tggcggattc tgacgttggc
    20701 gcatcgaaag gtggtctgtt tgataacagc aaaacgctta gcaaattgat tggctgccca
    20761 acgacaacgt tagccgaaag cgtaagccat ctttttaatg ttaataacta gttaattaaa
    20821 gtggcatcct cccgcatcct ctctgataat gacgggatgc cgggagcaat catgtctgct
    20881 tcctgaacgt ttcttctgac agaccaatgg atgccagtaa tgattagcgg cgtgctgtac
    20941 gccctgttag cagggttgat gtgggggctt atttttgtcg ggccgttgat cgtgccggaa
    21001 tacccggcga tgttgcagtc gatggggcgt tatctggcgt tagggttaat tgcgctaccc
    21061 attgcctggc tgggacgcgt gcgtctgcgt cagttggcgc gtcgggactg gcttaccgcc
    21121 ttgatgctca ctatgatggg caacctcatt tattacttct gccttgccag tgccattcaa
    21181 cgtactggcg cgcctgtttc cacgatgatt atcggcaccc tgccggtggt gattcctgtc
    21241 tttgccaatc tgctttatag ccagcgcgac ggcaaactcg cgtggggaaa actcgccccg
    21301 gcactgattt gtattggcat cggcctggcg agtgtgaata ttgctgagtt aaaccacgga
    21361 ctccccgatt ttgactgggc acgttatacc tctggcatcg tgctagcgtt agtttccgtg
    21421 gtctgctggg catggtatgc cctgcgcaac gcccgctggc tgcgggaaaa tcccgacaaa
    21481 catccgatga tgtgggcgac ggcgcaggcg ctggtcacgc tgccggtttc tctcatcggc
    21541 tatctcgtcg cctgttactg gctgaatata caaacgccgg acttctcctt accttttggc
    21601 ccccgtccgc tggtgtttat tagtctgatg gttgcgatag ccgtgctttg ctcatgggtt
    21661 ggcgcactct gctggaacgt cgccagccag cgattaccga cagtgattct cgggccgctg
    21721 attgttttcg aaacgctggc aggtttgctg tacacctttt tactccgcca gcaaatgccg
    21781 ccgctaatga cgctgagcgg tatcgcgctg ttagtgattg gcgtggtcat cgcggtcaga
    21841 gcaaaaccag aaaaaccttt aactgaatct gtctcagaaa gttgacatgc tggcagtgag
    21901 ttaaataagc ctctgctacg taagggttat agcttttgcc ttaaaggtgt atttaaaata
    21961 catcttatct tattaagaat gaggtatcag ctatggctta tcgcgaccaa cctttaggtg
    22021 aactggcgct ctctatccct cgcgcttcgg ctctgtttcg taaatatgat atggattact
    22081 gctgtggcgg taagcagacg ctggcgcgcg cggcggcacg taaagaactg gatgttgagg
    22141 tcattgaagc tgaactggca aagcttgctg aacaaccgat tgagaaagac tggcgtagcg
    22201 ccccgctggc agaaatcatt gaccatatca tcgtgcgcta ccacgatcgt caccgcgagc
    22261 agctgccgga gctgatcctg caagcgacta aagtcgagcg cgttcacgcc gacaaaccga
    22321 gcgtgccaaa agggctgaca aaatacctga ccatgctgca tgaagagctt tccagccaca
    22381 tgatgaaaga agagcagatc ctcttcccga tgatcaaaca aggcatgggc agccaggcaa
    22441 tggggccaat cagcgtaatg gaaagcgagc acgatgaagc gggcgaactg ctggaagtga
    22501 tcaaacacac taccaataac gtcacaccgc cgccagaagc ctgcaccacc tggaaagcga
    22561 tgtataacgg cattaatgaa ctgattgatg acctgatgga tcacatcagt ctggaaaaca
    22621 atgtactgtt cccacgcgcc ctagcgggtg agtgatctaa aagctggatg gcattgcgcc
    22681 atccagcatg ataatgcggt tatttccgca gttcagcagc ccgcttctta ccaataaaca
    22741 gccagcccaa ccccagcgcg ataaaccaca gcggggtaac cagcagcgcc tggcgagtgt
    22801 catcttccag tgtcagcaac acgaccacga acacaaagaa cgccatacat acccagcaca
    22861 tcagcttgcc gagcggcatc ttgtagatcg acttttcatg cagatgagga cgctgtttgc
    22921 ggtacaccag gtacgagcaa aggataatcg tccagacgaa cataaacaga atcgcggaaa
    22981 cggttgtaat catcgtgaac gcgccaatca cgctaggatt cacatacaac atcaccacgc
    23041 cgccgagcag acagatacac gagaaggtca accctttcgc gggtactgcg cgcttagaaa
    23101 gtttagcgaa cgctttcggc gcaacacctt cctgcgccag accaaacagc atacggctgg
    23161 tagagaagac accgctgtta gcggaagacg ccgcagaggt cagcaccaca aagttgatca
    23221 cgctggcagc cgcaggcaaa cctaccaaca caaacagctc aacgaacggg cttttctccg
    23281 ggactaccga actccacggt gtcacggaca taatcacaat cagcgcgaag acgtagaaca
    23341 taatgatacg gatcggaatg gagttaatcg cgcgtggcag tgatttctcc ggatctttgg
    23401 tttccgcagc tgttgtacct accagctcaa tccccacgaa agcgaaaacc gctatctgga
    23461 atccggcaaa gaagccactt aaacctttcg ggaaccagcc gccgtcattc cacaaatgcg
    23521 cgaatgacgc ttccacaccg gtcggtgact gaaagtgcat cgccaccatg accaggccga
    23581 cgacaatcag cgacacgatg gcgacgattt tgatcatcgc aaaccagaac tccatctcac
    23641 cgaacatttt cacggtggca aggttgagcg tcagcagcag cactatcacc gccagcgagg
    23701 cgacccagtc ggagagatcg gggaaccaga actgagcata agccgtgatc gccaccacgt
    23761 ctgccatacc ggttacaacc cagcagaacc agtaagtcca gccggtgaaa tatcccgccc
    23821 acggccccag taaatcggaa gcgaagtcac taaaagattt gtattccaga ttcgaaagca
    23881 gcaattcccc cattgcccgc atcacgaaaa agagcataaa accaatgatc atataaacga
    23941 aaatgatcga cggcccggca aggctaatcg ttttgccgga ccccataaac aacccagtac
    24001 caatggcacc tccaatggca ataagctgaa catgtcggtt tgtgagattg cgccgtagcg
    24061 actgttcagc cggagcctga tcatcggcaa cgacttttac ctgatctacc atgttttttc
    24121 ttcctgtacc tgtctgtgtt gttcaggctc tacggccttt agtcagtcta tgatacgacg
    24181 aatctgtatc gggactcatc gatattaggt aacaatacgc gggatgaata ctaagattta
    24241 gatctaatgt taattttatg tttaaagtga gtgtcatatc acggtgataa cgcgaaacag
    24301 ctcacaaaaa tacacgctta cggggtcttt gcaacacaaa atagcgccgt ttggcaaact
    24361 gaagggttta ttgctgaatg cctgctcccc tctggttcga ggggaacaga atgcgctgct
    24421 tagaggattt ccagcagttc gacttcaaac accagggtgc tgaacggagg gatggatgca
    24481 cctgcgccgc gctcgccata tgccagttcc tgcgggatag tcagttccca tttagaacct
    24541 accggcatca gagtcagtgc ttcaatccag ccagggatca cgccattaac cgggaattca
    24601 gcgggttcac cacgagcaac ggagctgtca aacacggtgc cgtcgatcag tttaccggtg
    24661 taatgaacac gaacgcggtc ggtacgtgcc ggaattgcgc cttcaccctg gttgatcacg
    24721 cggaattgca ggccagattc ggtgctattc acaccttctt ttttggcgtt ttcttccagg
    24781 tatttcacgc cttcagcagc catcgcctgg aaacgctgac gacgaacggc atcggcgcgt
    24841 tcgtggattt cacgcagcgc gcgatgcacc acatcaaccg gcacagccgg atgtttgcct
    24901 tccagcgcat cggcaatacc tgcaaccagt gcttctggca gcagcccttc caggccagat
    24961 tcactcagtt gttgccctac ctgcaaacca atgccgtagc ttgcttgcgc ttcgatggtg
    25021 tcaaaagttg gggtggtcat cgtatttcct ttcttagggt ataaaagtag cgggcagcat
    25081 atcagccaca cttctttggg taaaactttg tcgcaggaat gatgacaaat cccggggaaa
    25141 aagaaacaat agcaatatcc cgtatgaata taataaaggc gggtttacca cggacatcat
    25201 agcgttagct tattcatagg ctatgattga ggaacaagac gtggagcagg aggaaaccat
    25261 gcccgggcgc tttgaactaa aaccaaccct ggagaaagtc tggcacgcgc cggacaattt
    25321 tcgcttcatg gacccgctgc cgccgatgca tcgtcggggg atcattattg ccgccattgt
    25381 attggtggtc ggatttctgc ttccatctga tgatacgccc aacgcgtcgg tggtgacgcg
    25441 tgaagcgcag ttagatattc agtcgcaatc acaaccgccg acagaagaac agccgcaaac
    25501 tacccaaacg caaccattcc agccagatag cggaattgat aatcaatggc gctcttaccg
    25561 tgtcgaaccc ggtaaaacga tggcgcagct gttccgcgac cacggactgc ccgctaccga
    25621 tgtctatgca atggcgcagg tagaaggcgc gggtaagccg ctgagtaatt tgcaaaatgg
    25681 gcagatggtg aaaatccgcc agaacgccag cggcgtggtg acgggcttaa ccattgatac
    25741 ggggaataat caacaggtcc tgtttacccg ccagccggat ggcagtttta ttcgtgcgcg
    25801 gtagatgaag ggtcaaccaa aagccagcta aggattgtct ctttgatgac cttacgtctg
    25861 gcttccagtg cctgggggga aaccatactg atatgataaa ctaggccaag cgtatgctgg
    25921 ttgatcaaat aatatccccc aagcgcagca atattaatat tcacctgcaa tgggtcgata
    25981 cctggcttaa aaatattgta tttcttccct tcatccaata aggattccat taactgcaag
    26041 tgagcatggt taatttctaa gagacgctgt gattttgcat aatgcacacc tttactttga
    26101 ttttcactat gcacaatttt taaaaaccaa ggattagcaa tataataatc ccatgtaaaa
    26161 tcaataattt tctcaatggc tttttgtggc ggaagatctg agaaatcaag ggccttttct
    26221 ttttgtcgaa tatcgttcca ggtatattcc agcacttcga taaataaatt ttctttatta
    26281 ccaaagtggt gataaaccgt ctgtttacta caccctgctg cctttacaat attatctact
    26341 ctggcccctt cataaccata ttcggcaaat tcattaaccg cacatagcag caatttttcc
    26401 ttcaggctct gtacgttgct ggtatcaata tacataacgc ttccccacga cctcttcgtg
    26461 gtgactatca taacctgatt tgatttcagc ttcagaaaaa agggcaaaaa cttgtacttt
    26521 catcaggtta tcaaatgtcc tgtgatcgcc atcgcatatc aaccaactag atggttgatc
    26581 tttttatcct ccagtcgcaa gctaatcatc accaaggaaa caaaattcaa aaaagagagc
    26641 tattgtatga aatccactcg tcctgttgcc gtgattaccg gagccactcg cggtattgga
    26701 aagggctgtg cgcaggagct tgctcgtggc gggtttaacc tgctgattaa cgatcgtccc
    26761 gatgccgaca gcgtagaaaa actgcatatc acacaacaag aatgtttggc ggaaggcgtg
    26821 gaagtgattt gcttccctgc tgatgtcggt gatctatctc tgcatgagga gatgctcgac
    26881 gcggcgcaaa accagtgggg gcggctggac tgtctgctca ataacgcggg gatttcagtg
    26941 aaaaaacgcg gtgacctcct cgacctcgaa ccagacagct tcgatcaaaa cattgccatt
    27001 aacacccgcg cgccgttctt ccttgctcag gcattcagta agcgcttact ggcacagccg
    27061 aaaccggaag cggagctgcc gcaccgctcg attattttcg tcagttccat caacgccatc
    27121 atgctggcga tgaatcgcgg cgaatacacc attgccaaaa ccgccgtttc tgccgctgct
    27181 cgtctgtttg ctgctcgcct ttgtaatgaa caaattggcg tttatgaagt gcgccctggt
    27241 ctcatcaaaa ctgatatgac tattccggca acggcttatt acgacgaact gattgccaaa
    27301 ggtctggtgc cgtggggacg ttggggttat ccggctgata ttgcttccac cgtccgcgcg
    27361 atggcggaag gtaaactgat ttacacctgt ggtcaggcgg tcgcaatcga cggcggctta
    27421 agtatgccgc gcttctgagg ctgaatcatg agagatctgt tacaacgccc ggatttattc
    27481 agtatcaata ccgcgacact gggctataaa acgccgctac ccgccattat tgatgcctgc
    27541 gccgcacgcg gtattggcgc aattgccccc tggcgcaggg agttgcaggg tgaagatctc
    27601 cagcaaatca cccgccagct tgccgccagt aacatgagcg tttccggttt gtgccgtagc
    27661 acttactaca ccgcgccgac actggcggaa cgcaaactgg cgattgatga taaccgccgg
    27721 gcgctggacg acgcagcggt gcttaacgcc gcctgttata tgcaggtggt tggcggtctg
    27781 ccaacgggca ctaaagatct atatgaagcg cgagaacaag tgaaacaagg gattcgccag
    27841 ttgctgccac attcgaaaga tgtcggcgtg ccaattgcgc tggagccact gcacccgatg
    27901 acagctgccg atcgctcttg cctgtgcacg ttacgtcagg cgctggactg gtgtgatgaa
    27961 ctcgatcccg atggtgagtt tggcctcggc gtggcggtgg atgtttatca cgtctggtgg
    28021 gatccggatc tcgccagtca gatcctgcgt gccggaaaac gcatcctcgc gtttcatgtt
    28081 tccgactggt taatgccgac cactgatctg gtcaatgacc gtggtatgcc gggagatggc
    28141 gtcatcaata ttccgtcaat tcgtcggctg gttgaaaacg ccggatttaa tggtgctatt
    28201 gaactggaaa tattctcgcc ttactggtgg cagaaagata ttaacag
//
LOCUS       DAJTRB010000054        28139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTRB010000054 DAJTRB010000000
VERSION     DAJTRB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.054"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            564..1334
                     /locus_tag="OO330_002394"
     CDS             564..1334
                     /locus_tag="OO330_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5883233.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO330_002395"
     CDS             1325..1798
                     /locus_tag="OO330_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5883234.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO330_002396"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO330_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5883235.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO330_002397"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO330_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5883236.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO330_002398"
     CDS             3233..3727
                     /locus_tag="OO330_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5883237.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OO330_002399"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OO330_002399"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5883238.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFNGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO330_002400"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO330_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="OO330_002401"
     CDS             7571..8485
                     /locus_tag="OO330_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5883239.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..9766
                     /locus_tag="OO330_002402"
                     /pseudo
     CDS             8700..9766
                     /locus_tag="OO330_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9815..11479)
                     /gene="tsr"
                     /locus_tag="OO330_002403"
     CDS             complement(9815..11479)
                     /gene="tsr"
                     /locus_tag="OO330_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5883240.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11598..12044)
                     /gene="hpaR"
                     /locus_tag="OO330_002404"
     CDS             complement(11598..12044)
                     /gene="hpaR"
                     /locus_tag="OO330_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5883241.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12316..13605
                     /locus_tag="OO330_002405"
     CDS             12316..13605
                     /locus_tag="OO330_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5883242.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAIWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13602..15068
                     /gene="hpaE"
                     /locus_tag="OO330_002406"
     CDS             13602..15068
                     /gene="hpaE"
                     /locus_tag="OO330_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5883243.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQDYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMHVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15070..15921
                     /gene="hpaD"
                     /locus_tag="OO330_002407"
     CDS             15070..15921
                     /gene="hpaD"
                     /locus_tag="OO330_002407"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5883244.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15931..16311
                     /locus_tag="OO330_002408"
     CDS             15931..16311
                     /locus_tag="OO330_002408"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5883245.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16379..17182
                     /gene="hpaH"
                     /locus_tag="OO330_002409"
     CDS             16379..17182
                     /gene="hpaH"
                     /locus_tag="OO330_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5883246.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17193..17996
                     /gene="hpaI"
                     /locus_tag="OO330_002410"
     CDS             17193..17996
                     /gene="hpaI"
                     /locus_tag="OO330_002410"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5883247.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18018..19373
                     /gene="hpaX"
                     /locus_tag="OO330_002411"
     CDS             18018..19373
                     /gene="hpaX"
                     /locus_tag="OO330_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5883248.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19383..20273
                     /gene="hpaA"
                     /locus_tag="OO330_002412"
     CDS             19383..20273
                     /gene="hpaA"
                     /locus_tag="OO330_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5883249.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPSNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20300..21237
                     /locus_tag="OO330_002413"
                     /pseudo
     CDS             20300..21237
                     /locus_tag="OO330_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21464..23022
                     /gene="hpaB"
                     /locus_tag="OO330_002414"
                     /pseudo
     CDS             21464..23022
                     /gene="hpaB"
                     /locus_tag="OO330_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23040..23552
                     /locus_tag="OO330_002415"
     CDS             23040..23552
                     /locus_tag="OO330_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5883250.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23945..26095
                     /gene="btsT"
                     /locus_tag="OO330_002416"
     CDS             23945..26095
                     /gene="btsT"
                     /locus_tag="OO330_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5883251.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26145..26348
                     /locus_tag="OO330_002417"
     CDS             26145..26348
                     /locus_tag="OO330_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5883252.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26359..27315
                     /gene="yjiA"
                     /locus_tag="OO330_002418"
     CDS             26359..27315
                     /gene="yjiA"
                     /locus_tag="OO330_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5883253.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27384..>28139)
                     /locus_tag="OO330_002419"
     CDS             complement(27384..>28139)
                     /locus_tag="OO330_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5883254.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6903 a         7357 c         7037 g         6842 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgatttta
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgagcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc gcgggatgtt
     9001 tattaccatt ggtgtactgg ggatttttct cgccatcggc tggtatatgc tctatcgcaa
     9061 ccgcgagcac gtagaactga ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa
     9121 tgcccgccga gatccgctca attttgccga atggcgcagc ctgttccgta accgcacaat
     9181 gtggggaatg atgctcggat tcagtggcat caactacact gcgtggctgt atctggcctg
     9241 gcttcctggt tacctgcaaa cagcctataa cctggattta aaaagcacag ggttgatggc
     9301 ggctatccct ttcctgtttg gggctgccgg gatgctggtc aacggttacg ttactgactg
     9361 gctggtcaaa gggggaatgg ctccgattaa aagccgtaag atctgcatta ttaccgggat
     9421 gttctgttct gccgccttta cgctgatagt accacaagcg acaacatcca tgatggcggt
     9481 tctgctgatt ggcatggcac tgttctgtat tcactttgcc ggaacatcct gctggggctt
     9541 gatccacgtc gcagttgctt ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc
     9601 cagcttcatc tgcgcctctt tcgcgccgat cattactggt tttattgttg atacaaccca
     9661 ctcattccgt ctggcactaa tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta
     9721 catcttcctg gttcgccagc cgatcaacga cccgcgtaaa gattaaactc cctatgcccg
     9781 ataacatttt gcttatcggg catttttatg gcgattaaaa cgtttcccag ttctccccgc
     9841 tatctgccac tgccatttta cgcggcgtag ctggcgtcac ggtttttacc acagccgatg
     9901 tttcacgctg ctgttgctgc tgttgctgaa tccggaacac tgccacagct tcggtcaggc
     9961 gactggcctg ctcttccagc gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt
    10021 tctgttgagt tacccggtcc atctcagcaa ccgctaagcc aacctgatcg ataccacggc
    10081 tctgctcatc agaagcagaa gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac
    10141 tgacaatctc cgccattgtt tccccggcgc tttcgaccag cgtagagcca acatccactt
    10201 tccccaccga gtcttcaatc aggcttttaa tttcacgaac cgcctgggcg ctacgctggg
    10261 ccagattacg cacttctccc gcaaccaccg caaaaccacg tccttgctca cccgcacgcg
    10321 ctgcttcaac cgccgcgttc aaagccagaa tattggtctg gaaggtaatg ccgtcaatta
    10381 cgctgataat atcggcgatt ttctgcgaac tggtggagat atcgcgcata gtctgcacca
    10441 cgttatctac cactttgccg ccgcgttgcg ccgtttcaga agcacttaat gccagatggc
    10501 tggcctggcg cgcattctcg gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg
    10561 ctgccgtctc ttccagcgaa gcggcctgtt gctcggtgcg cgaagagaga tcattattgc
    10621 cggtagcgat ttcgctggca ccgctataga tggcattggc cccgttgcgc acatcaccga
    10681 cggtacgcat cagctctccc tgcatatggc gcaaactctc tgccagttgc cccatctcat
    10741 tagagccatc cacctcaatc ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt
    10801 caatcaggcg attcattggc gctaccagcg aggctttaat accgaaccag acggcgaaga
    10861 tgaccgccag tacgacgatc atcacgccca ccagaatcca catcgcctgg ctgtaggagg
    10921 cattgttatc gctgacggcg atatcataga gccgatcgtt ttgctccatg taagccacat
    10981 actgcttctc gaaaccgtcc tgatatccct gggtcggctg atcaaagaac tcgttgattt
    11041 tgcctgcacc taacagttgg atcagctccg ccagcgcatt gtgataaata tcgtaattac
    11101 gtttgatctc tgccgctgcg gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat
    11161 ccgcccagtt tttttccgcc tgtttcagcg aaatactggc actctgcatc agctcagcaa
    11221 cggttgaacc gctaccaata ttattctgat ccatcatgta gcggataccc gcgcggttga
    11281 gggtgttacg cgtctgcaac aacgcgaccc agctgccatt cagcgtgggt tgctgctggc
    11341 gaatggtttg taaaacagtg aaattttctt tgtcattctt taaggcatta aaaaacagac
    11401 cgcctgatgt cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag ctggtcacga
    11461 tcttgatacg ttttaacatg gtttctcttt cctgtggacc gatgcaagat tttcggcctg
    11521 gaaagcaaaa actttatgaa aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt
    11581 tatcagatac taaaaagtta ttcattatcg cccgggatgt cttcttgtcg ggaattgccc
    11641 agagcaataa attcctctaa cagatgggtt aactgttgca ttttctcggc agtaaactga
    11701 gcttcaatct gccgataggc ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc
    11761 ccctctttgg tcagcgagat atagagcttc cgctgatcat tgatcggctt taatcgcaac
    11821 actaaaccgt cgcgttccat ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag
    11881 gcgcgatacg ccagatcgtg aaaatccatt gatgggcttt ccgccagaat acgcacgatg
    11941 cgccactgtt gctcagtcaa attatgccgt ttaacgattg ggcgaaaata actcatcgcc
    12001 gcttctcgcg cctggagcaa cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa
    12061 aattcaaaat cattaatata gaaacaatta atcatcacgt taccgggact gagctgcccc
    12121 cgcaattctt cattttatca aaattgatga tattttatta acttactgat atttaatatt
    12181 ttattatctt taaatgtaaa tagtttgtta attagatcac atttatatca cttacttttg
    12241 ccaaactacg caatcgggaa taaaagtaat cattaacata ttaatgatta agatgataga
    12301 atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca tcgcagccaa
    12361 cttgatgcct ggcaggaagc gttccagcaa tctccctaca aagccccgcc taaaactgcg
    12421 atctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat tccctttcca
    12481 cagggtgaaa aagtactgag cggtgcgact gttgcgctga ttgtggggaa aacggcgacg
    12541 aaagtacgtg aagaagatgc ggcagagtac atcgccggat atgcgctggc taacgacgtc
    12601 agcctgccgg aagagagctt ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc
    12661 tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgactat ctataccgag
    12721 atcaacgggc gtcctgccga tcactggaat accgccgatt tacaacgtaa cgccgcacag
    12781 ctgctgagcg ccctgagcga atttgccacg ctgaatccag gcgatgctat tctgctcggc
    12841 acgccacagg cgcgcgtgga aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt
    12901 ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg caagagcttc
    12961 ccaacgctgc cacacccgca cggtacgctg tttgccctcg gcctgaacta cgccgaccac
    13021 gccagcgaac tggaatttaa gccaccggaa gagccgctgg tgttcctgaa agcgccgaat
    13081 accctcactg gcgacaacca gacctccgtg cgcccgaaca atattgaata catgcactac
    13141 gaagcggagc tggtggtagt tattggcaag caggcgcgta acgtcagcga agccgatgcc
    13201 atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgcga ctatctggaa
    13261 aactactacc gccctaacct gcgggtcaaa agccgcgacg gactgacgcc gatgctttca
    13321 accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg caccttcgtc
    13381 aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt gcccttcctg
    13441 atcgcctact taagcgaatt tatgaccctg aatccgggcg acatgatcgc caccggcaca
    13501 ccaaaaggct tatctgacgt ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg
    13561 ggccgcctgg tgaaccgaat tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt
    13621 ggatcaacgg caaaaatgtt gcaggtaacg actacttcca gactaccaat ccggcaacgg
    13681 gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg
    13741 cagcgaaaga ggcgttcccg aaatgggctg atctgccgat gaaagagcgt gcgcgcctga
    13801 tgcgccgtct gggcgatctg atcgaccaga acgtgcctga gatcgccgcg atggaaaccg
    13861 cggacacggg cctgccgatc catcagacca aaaatgtgtt gatcccacgc gcttctcaca
    13921 actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacctat ccggttgacg
    13981 acaagatgct caactacacg ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt
    14041 ggaacgtgcc gtttatgacc gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata
    14101 ccgcggtgct gaagatgtcg gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg
    14161 cgctggaagc tggtattccg gcgggcgtgc taaacgtggt acaggactac ggcgcaaccg
    14221 caggcgatgc gctggtccgt catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg
    14281 ccaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg gagctgggcg
    14341 gtaaatcacc ggtgctgatt tttgaagatg ccgatattga gcgtgcgctg gacgccgccc
    14401 tgttcaccat cttctcaatc aacggcgaac gctgcaccgc cggttcgcgc atctttattc
    14461 agcagagcat ctatccagaa ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg
    14521 tgggcgatcc gaacgatccg aatacccagg ttagggcgct tatcagtcag caacactggg
    14581 aaaaagtctc cggctatatc cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg
    14641 gcccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa
    14701 cggtgctggc ggacgtagat aaccgtatgc acgttgccca ggaagagatt ttcgggccgg
    14761 tcgcctgcct gctgccgttt aaagacgaag ccgaaggctt acgcctggca aacgacgtgg
    14821 agtacggcct cgcgtcgtac atctggacac aggatgtcag caaagtgtta cgcctggcgc
    14881 gtggcattga agcaggcatg gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc
    14941 catttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag
    15001 tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt ccgaaatggg
    15061 gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc gtcgatgtat
    15121 ctctctgaac taccaggaaa aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa
    15181 gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt cgatactcac
    15241 tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg cgtctacacc
    15301 agcaacgagc tgccgcattt tattcgcgac atgacctaca actacgaggg caacccggag
    15361 ttggggcagc taattgccga ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat
    15421 attcccagcc tgaaactgga atacggcacg ctggtaccga tgcgctacat gaatgaagac
    15481 aagcacttca aagtggtctc catttcggct ttctgcacgg ttcacgattt tgccgacagc
    15541 cgcaagctgg gcgaagcgat tctgaaggcg atcgaacagt acgacggcac cgtggcggtc
    15601 cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgtgcgga agaagggatg
    15661 aacagctaca cccgcgagtt cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc
    15721 gaaggccagt tcaaagagtt ttgcaatatg ctgccggagt acgccgacta ctgctacggc
    15781 gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcggctggga taaatacgac
    15841 ggcaaggtgg agtttattac cgagctgttc ccaagctctg gcaccggtca ggttaacgct
    15901 gttttcccgc tgcccgcgta aggacgtttt atgccgcact ttatcgtgga atgtactgaa
    15961 aatattcgtg aagaagcccg cttacctgag ctgtttgcca gtgttaacac tgcactggcg
    16021 gcaacgggga tttttccgct tgggggaata cgtagccggg cgcactggat cgatacctgg
    16081 caaatggctg atggcaagca cgattacgcc tttgtgcata tgacgctaaa aatcggcagc
    16141 ggacgcagtc tggaaagccg tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg
    16201 cacttcgcct cactgatgga gagccgttac ctagcgcttt catttgagat tgcagagctg
    16261 cacccgacgt tgaatttcaa acagaacaac gtacacgcgt tgtttaaata aatttgcacc
    16321 agcttccagg ccggatgata aggcgctgcc gcatccggca atgccacagg atatcgctat
    16381 gttcgataaa cacacccaca ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg
    16441 cgaacagatc cgcgcgatct cgctggatta cccggagatc accatcgaag acgcctacgc
    16501 cgtgcagcgc gaatgggttc gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa
    16561 aatcggtctg acctcgaaag cgatgcaggc aagctcgcag atcagcgaac cggattacgg
    16621 tgcactgctg gacgacatgt tcttccacga tggcagcgat atcccgaccg atcgctttat
    16681 cgtgccgcgc attgaagtgg agctggcttt tgtgctggca aaaccgctgc gtgggccaaa
    16741 ctgcacgctg ttcgacgttt acaacgccac ggactatgtg atcccggcgc tggagctgat
    16801 cgacgctcgc tgccacaaca tcgatccgga aacccagcgc ccgcgcaaag tattcgacac
    16861 catttccgat aacgccgcca acgccggagt gatcctcgga ggtcgtccga tcaaaccaga
    16921 cgaactggat ctgcgttgga tctccgccct gatgtatcgc aatggcgtga ttgaagaaac
    16981 cggcgtcgcc gctggtgtgc tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa
    17041 actcgcaccg tatgacgtac aactggaagc cgggcaaatc attctcggtg gttcgttcac
    17101 ccgcccggtt ccggcgcgta agggcgacac cttccacgtc gattacggca acatgggctc
    17161 cattagctgc cgctttgttt aaggagagaa cgatggaaaa cagttttaaa gcggcgctga
    17221 aagcaggccg tccgcagatt ggattatggc tggggctgag cagcagctac agcgcggagt
    17281 tactggccgg accaggattc gactggttat tgatcgacgg tgagcacgcg ccgaataacg
    17341 tgcaaaccgt gctcacccaa ctacaggcga ttgcgcccta ccccagccag ccagtggtac
    17401 gcccatcgtg gaacgatccg gtgcaaatca aacaactgct ggacgtaggc acacaaacct
    17461 tactggtgcc gatggtacaa aacgccgacg aagcccgtaa agcggtacgc gccacccgtt
    17521 atccccccgc cggtattcgc ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc
    17581 gcattcctga ttacctgcaa aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa
    17641 cgcgtgaggc aatgaagaac ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt
    17701 ttatcggccc ggcggatctg agcgccgata tgggttatgc cggtaatccg cagcacccgg
    17761 aagtacaggc cgccattgag caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg
    17821 ggatcctgat cgccaatgag caactggcaa aacgctatct ggaactgggc gcgctgtttg
    17881 tcgccgtcgg cgttgacacc accctgctcg cccgcgccgc cgaagcgctg gcagcacggt
    17941 ttggtaatag ttcttctatt tcgatggcga agcaaaacaa caactctgtc tactaaacgt
    18001 acgatgtgga gcgcatcatg acgacctcta ccctgcaata taaagataat aaaactgttg
    18061 aaattgaaca tcgtgtcatt aataaattat ttcgtcgcct aatcattttt ctttttattc
    18121 tgtttgtttt ttcgtttctt gatcgcatca atataggatt tgccggactg acaatgggca
    18181 aggatcttgg cctgacgtcc acaatgtttg gcctcgccgc aacactcttt tatgtcacgt
    18241 acgtcatgtg cgggatcccc agtaatatga tgttagcgaa aattggcgct cgtcgatgga
    18301 tcgctgggat catggtggtc tggggaattg cttccacctg cacaatgttt gcaacaagcc
    18361 ccgaaacact ctatctactg cgtatgctgg ttggtattgc tgaggcgggt tttttacctg
    18421 gtattttggt ttatctgacc tggtggttcc cggcatatca tcgggccaga gccaatgcct
    18481 tatttatgat agcgatgcca gtcactatga tgctgggatt gatcctttca ggctacattc
    18541 tggccttgga tggactgtgg aatctaaaag gctggcaatg gctctttttg ctggaaggtc
    18601 tgccatcggt cattcttggt gtcgtcacct ggttttatct caacgacaca ccagaccagg
    18661 cgacctggct tgatgaaaac gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag
    18721 agagtgcatt tgcccatgca tctgttccac gctcaacctt acgtgaagtc ctgaccccag
    18781 ccatcatgct atatacactt gcttattttt gcctgactaa tactctgagt gcgattaata
    18841 tctggacgcc gcaaatccta cagagtttca atactggcag tagcaacatc atgattggtt
    18901 tgctggcggc catcccccaa ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt
    18961 ccgaccgtct taaagaacgc aaaaagcata cgattcttcc ctacctgttt gccgctgccg
    19021 gatggttact cgcttctgcg acagatcaca gcctcattca actgagcagc atcattatgg
    19081 cctcaatggg atcatttacc gcgatgacta tcttttggac aacaccggat caagttataa
    19141 gcttgcaatc gcgtgccgtt gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag
    19201 cagtcagtcc attacttatc ggtattttga aagatgcaac cggcagtttt agctcaggtt
    19261 tgtggtttgt agcaggattg ctggtactgg gcgcgttagt catgacccga atccctatgg
    19321 ggagtcggga acactctctt tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca
    19381 tgatgtccag tgaagctatc tttgaacatg gttatgaaaa tattgatttg gtccgggaat
    19441 atgatgcccg ctacgctggt gaagatgtgc attatgaaac atttgcccgg ttagcgaatt
    19501 tctttggacg cgatatgcgc cctcactggc acgatagata ttttcaactc cattatctgg
    19561 tcactggacg cattacttta caactggatg agcacttcta ttctgtgcgc gcaccgcttt
    19621 ttgtactaac accaccatcg gtgccacata ctttctttac tgatcctgat agcgatgggc
    19681 atgttctgac cgtacgtcag gagttaattt ggcccctggt agaaaagctc tggcctggta
    19741 gtggagatgc cgcaatgcta caaggtatct gtctgtcact ggagggaatg gaacatacac
    19801 aggcggcatt aaatcattat tggccgataa tatccctgga gtttcaacaa aataagcaag
    19861 gacgggaaat tctattgcaa tcactggcgc aaagtatttt tacaatccta ctgcgtgaag
    19921 caccatcaaa tgatatttca acatgcagcg tgcgcggcga gatgtgttta ttccagaagt
    19981 ttaatcggct gattgacgaa aattatcgcc aacatttaat ggtccctgaa tatgcaacaa
    20041 tgctcggtat gagtgaatca cggcttaccg agctgtgccg acgttttgcc aatcaatcgc
    20101 cgaagagatt gattttcgaa cgagtgagcc gggaagccag acggcttctg ctctatagcg
    20161 agcaaagcat caaccagata gcccttgatc ttggctacaa agatcccgcc tatttcgccc
    20221 gttttttcaa ccgaatgatg ggttgctcac caacacaatt ccgtggtcgc tgaccattta
    20281 tcaacctgag agactcgaca tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat
    20341 tgcgccagtg gcattcacct acgcctccga accgcaataa atgaaaattg tactacttgg
    20401 aacgaaaggt ggcccttctt tattaaaagc tgaccgttta ccgatggcaa cggcacttat
    20461 tattggcaat aaaacctggt taattgatgc tggttacggg gccagcttac aactggtaaa
    20521 aaataatatt ccactgcgcc agattgataa tattttactc acccatttac acagtgatca
    20581 tattctggac tatccatcag tcttaatgaa tgcatgggct agcggtctgc aggacaaaac
    20641 tattcatgta ttcggtccgc caggcacccg agccatgacc gctgccagtt ggcaggtctt
    20701 cgatcgggac atcacactgc gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt
    20761 tacagctcaa gatgttggtg aaggcgtagt ttataaagat gataccgtga ccgtgactgc
    20821 attaaaggtc cctcattctc ctttccctga cggtgaggct tttgcttatc gcttcgacac
    20881 acaaggcaag cgaattgtct tctcaggaga tacctcgtac tttcccccac tggcaacttt
    20941 ctcagaaggc gctgatatac ttgtgcatga ggccgtacat gagccctccg tcgctaagct
    21001 ggcagacagt attggcaatg gtaaaacact ggcaaaagcc attgcttctc accatacgac
    21061 tattgaggat gtaggcaaaa ttgccaatga agcaaaagtg aaaaaactca tccttagcca
    21121 cctggtacca atcaccgttc cggatgaaac atggaagcaa gaagcacaga aaactattca
    21181 ggccctatcg tcgttggtca tgaaaatatg acgattacca ttccattcgc cccataaaaa
    21241 attccgtccc cgttagccct tgttagcggg gaatattatg atttgcctta ctgtaagttg
    21301 atcacgatcg cagtttacta tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc
    21361 tgaaacgtct cttcccgaca accgcgttca acgcttcaat taaataacaa aaacaacaca
    21421 caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac cagaagattt
    21481 ccgcgccagt acccaacgtc ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg
    21541 tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc accactcatc cggcatttcg
    21601 taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg agatgcagga
    21661 ctctctgtgt tggaacaccg acaccggcag cggcggctat acccataaat tcttccgcgt
    21721 ggcgaaaagt gccgacgacc tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct
    21781 gagctatggc tggatgggcc gtaccccaga ctacaaagcc gctttcggtt gcgcactggg
    21841 cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt atacccgtat
    21901 tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg tcatttgccg
    21961 accgataaag tgaaagacgt ttacatcaag ctggaaaaag agactgacgc cgggattatc
    22021 gtcagcggtg cgaaagtggt tgccaccaac tcggcgctga ctcactacaa catgattggc
    22081 ttcggctcgg cacaagtgat gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca
    22141 atggatgccg atggcgtgaa attaatctcc cgcgcctctt atgagatggt cgcgggtgct
    22201 accggctcgc catacgacta cccgctctcc agccgcttcg atgagaacga tgcgattctg
    22261 gtgatggata acgtactgat cccatgggaa aacgtgctga tctaccgcga ttttgatcgc
    22321 tgccgtcgct ggacgatgga aggcggtttc gcccgtatgt atccgctgca agcctgtgtg
    22381 cgcctggcag tgaaactcga cttcattacg gcactgctga aaaaatcact cgaatgtacc
    22441 ggcaccctgg agttccgtgg tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac
    22501 accttctggg cattgagtga ctcgatgtgt tcagaagcga cgccgtgggt caacggggct
    22561 tatttaccgg atcatgccgc actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg
    22621 aagatcaaaa acattataga acgcaacgtt accagtggcc tgatctacct cccttccagt
    22681 gcccgtgacc tgaacaatcc gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg
    22741 aacggtatgg atcacgtcca gcgcatcaag atcctcaaac tgatgtggga cgccattggc
    22801 agcgaatttg gtggtcgtca cgagctgtat gaaatcaact actccggtag ccaggatgag
    22861 attcgcctgc aatgtctgcg ccaggcacaa agctccggca atatggacaa gatgatggcg
    22921 atggttgatc gctgcctgtc ggaatacgac cagaacggct ggactgtgcc gcacctgcac
    22981 aacaacgacg atatcaacat gctggataag ctgctgaaat aacgcagcag gaggttaaga
    23041 tgcaattaga tgaacaacgc ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg
    23101 taaatattat caccaccgag ggcgacgccg gacaatgcgg gattacggca acggctgtct
    23161 gctcggtcac ggatacacca ccgtcgctga tggtgtgcat taacgccaac agtgcgatga
    23221 acccggtttt tcagggcaac ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac
    23281 tgatggcgcg ccacttcgcg ggcatgacag gcatggcgat ggaagagcgt tttagcctct
    23341 catgctggca aaaaggtccg ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg
    23401 aaggagagat ccgcgatgtg caggcgattg gcacacatct ggtgtatctg gtggagatta
    23461 aaaacatcat cctcagtgca gaaggtcacg gacttattta ctttaaacgc cgtttccatc
    23521 cggtgatgct ggaaatggaa gctgcgattt aagctccagc cccttcacgc attcgtcgaa
    23581 ggggcatttt tattgtgatc tcaatcacat atctacccga tgaataattc ttgcccaacc
    23641 gctcaccgcc attcctgacc gcttacatcc ctaaaataac cactcagtta tttaccttac
    23701 tttacgcgca cgtatctctg gcaacatcac tgcaggatag cggtcaattt acctcctcaa
    23761 acgcaacgca aacctagaac ggcttcggcc tatgattaac aaattactct atggattttg
    23821 gtttgcagga aggcagcaag tgagtgaatc cccgggagct tacaaaagta agtgtcagag
    23881 gtgaacgaac gcagctgccg cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt
    23941 tactatggat acgaaaaaac tattcaagca cataccctgg gtgattctcg gaatcatcgg
    24001 tgcattctgc ctcgcggtag ttgcattacg tcggggggag cacgtcagcg ccctgtggat
    24061 cgtggtcgcc tctgtgtcgg tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc
    24121 ccagaaggtg atgaaactcg accccacgcg cgcgacgcct gcggttatta acaacgacgg
    24181 tctgaactac gttccgacca accgttacgt gttgtttggt caccacttcg ccgctatcgc
    24241 cggtgctggt ccgctggtgg gaccagttct cgccgcgcag atgggctacc tgcctggcac
    24301 gctgtggctg ctggcggggg tagtgctggc cggtgcggta caggacttta tggtgctgtt
    24361 tatctcctct cgccgtaacg gcgcatctct cggtgagatg atcaaagaag agatggggcc
    24421 agtaccgggg actatcgcgc tgtttggctg tttcttaatc atgatcatca tcctcgccgt
    24481 tctggcgctg attgtggtta aagccctggc cgaaagtccg tggggtgtct tcaccgtttg
    24541 ctcaaccgta ccgattgcgc tgtttatggg tatctacatg cgctttatcc gtccggggcg
    24601 tgtgggtgaa gtctctgtta ttggtatcgt gctgctggtt gcctctatct acttcggtgg
    24661 cgtgattgct cacgatccgt actggggtcc ggcactgacc tttaaagaca ccaccattac
    24721 cttcgcgctg attggctatg cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc
    24781 accgcgcgac tatctggcaa ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg
    24841 tatcgtggtg ctgaacccgg aactgaaaat gcctgcgatg acccagtaca ttgacggtac
    24901 tggcccgctg tggaaaggcg ctctgttccc gttcctgttc atcaccatcg cctgtggtgc
    24961 ggtatctggc ttccacgcgc tgatctcttc cggtacgacg ccgaaactgc tggctaacga
    25021 aaccgacgcg cgtttcattg gctacggcgc aatgctgatg gagtccttcg tggcgattat
    25081 ggcgctggtt gcagcgtcca tcatcgaacc gggtctttac ttcgcgatga acaccccgcc
    25141 tgctggcctt ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat
    25201 catcatggcg cagctgaaag acgttaccgc acacgcggca gcgaccgtca gctcctgggg
    25261 cttcgtgatt tcgccagagc agatcctgca aaccgcgaaa gacattggtg agccttctgt
    25321 cctgaaccgt gcaggtggtg cgccgacgct ggcggtaggt atcgctcacg tgttccacaa
    25381 agtgctgccg atggctgaca tgggcttctg gtatcacttc ggtattctgt tcgaagccct
    25441 gttcatcctg accgcgctgg atgcgggtac ccgttctggc cgctttatgc tgcaagacct
    25501 gctgggtaac ttcatcccgt tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg
    25561 tactgcgggc tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg tcgatccgct
    25621 gggcggcgtt aagagcctgt ggccgctgtt cggtatctcc aaccagatgc tggcagccgt
    25681 agcgctggta ctgggcaccg ttgtgctgat taagatgaag cgcacccaat acatctgggt
    25741 aactgttgtt ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact
    25801 gttcagcacc aacccgcaga tggaaggctt cttctacatg gcaagccagt acaaagagaa
    25861 gattgctaac ggtactgacc tgacggcgca gcagattgcc aacatgaacc acatcgttgt
    25921 gaacaactac accaacgcag gcctgagtat tctgttcctg attgtggtgt acagcatcat
    25981 cttctacggt ttcaaaacct ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga
    26041 aacaccgtac gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg
    26101 tttagccccg cttcggcggg gctttgttct tatcagagtg aactatgttt ggtaacttag
    26161 gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt gattggtatt ccagactacg
    26221 acaactacgt cgagcatatg aagaccaacc atcccgacaa gccgtacatg agctatgaag
    26281 aattcttccg cgaacgccag aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct
    26341 gttgttaatg gagagagcat gaacccgatt gcagttaccc tactcaccgg ttttttaggc
    26401 gcgggcaaaa ctaccctgct gcgccatatt cttaacgaac aacacggcta caagattgcc
    26461 gtgattgaaa acgaattcgg cgaagtctcc gttgatgatc aactgattgg cgaccgcgcc
    26521 acgcagatca aaacgctgac caacggctgc atctgctgtt cgcgctccaa cgagctggag
    26581 gacgcactac tcgacctgct ggataatctc gacaagggca atattcagtt cgatcgtctg
    26641 gtgattgaat gcaccggcat ggccgatccc ggcccgatta ttcagacctt tttctcccat
    26701 gagattttat gccagctcta cctgctggac ggcgtgattg cgctggtgga tgcggtacat
    26761 gccgatgagc agatgaacca gttcaccatc gcccagtccc aggtgggcta cgccgaccgt
    26821 attctgctga ctaaaaccga cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg
    26881 cgcatcaacg cccgcgcacc ggtctacacc gtcacccacg gcgacatcga cctgggcctg
    26941 ctgttcaaca ccaacggttt tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc
    27001 cactttatcg cggataaaca aaacgatatt tcgtcaattg tggtggaact ggattacccg
    27061 gtagatatca gcgaagtttc ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa
    27121 ctgctgcgtt acaaagggat gctgtggatt gacggcgaac ctaatcgcct gctgttccag
    27181 ggcgtccagc gcctctacag cgccgactgg gacaggccgt ggggcgatga aaaaccgcat
    27241 agcacaatgg tgtttatcgg gattcagttg ccggaggaag agattcgggc ggcgtttgcg
    27301 ggattgagga agtaagttcg ggaaagatca actttttgag agttccaccg caacattatt
    27361 acgagtggaa ctctttagat atgtcagacc aaccaaagct tcttcaattg cgttttcatt
    27421 ttttcatgtc tcattttcag ttcagccaat ccccattctg aagaattaca aacatctttt
    27481 gttaaataaa aagagctttt actattaagt ttctcataaa tagatttctt tttgataaaa
    27541 tcatagtgtt gtgtttcaga atttttggaa ccacttatga gtgttaaatt acctatttta
    27601 tgcagccagt agacatgctc ttgaggttgg aatctattaa tccagtattc attaactaat
    27661 gcttgaggaa gaatatgctc aattgtgatt ctccccgtat aggttttaat cacgctctca
    27721 tcttgttgct ccatatctaa gcgaagcaaa attgctttta ttaagttcac ttgattcgga
    27781 cgaggctcat ataaatcttc atccagagca gctataaaac ctgaattatc tgcatgttgg
    27841 tttatctgat ttatcacaga atcaaaaggc atatcattat ttattgctac cagagcagaa
    27901 taacaaacca tttctctttg gcttttaatt tgttttttta accacccatg catatatacc
    27961 ttctcaaagg cggtaataaa ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc
    28021 attcgattca tgaatgccat aaccggagga atccactcat ctacccctaa attacttagc
    28081 gatgctattt ttcgtcgtat tgaagggtgt tcaaaatcat tctctaaaat cttagtata
//
LOCUS       DAJTRB010000055        27878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTRB010000055 DAJTRB010000000
VERSION     DAJTRB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.055"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            76..2289
                     /gene="tssI"
                     /locus_tag="OO330_002420"
     CDS             76..2289
                     /gene="tssI"
                     /locus_tag="OO330_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5883255.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHEGRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            2312..2757
                     /locus_tag="OO330_002421"
                     /pseudo
     CDS             2312..2757
                     /locus_tag="OO330_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            2779..3186
                     /locus_tag="OO330_002422"
     CDS             2779..3186
                     /locus_tag="OO330_002422"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5883256.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            3183..5660
                     /locus_tag="OO330_002423"
     CDS             3183..5660
                     /locus_tag="OO330_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5883257.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWSQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDRSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELVKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDEQGLAVSRTDARGGEVALAHDARGQLRRYTDCSGCATGYE
                     YDEDGNLTAVTDAEGKTVRISYNRQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGS
                     VQSWQYTPRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQ
                     RPDGLIRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNR
                     LDQITEVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTD
                     ITLPDGQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADN
                     RLSWQRSLPGGSQNPQQAVLPRRRTTA"
     gene            5657..6646
                     /locus_tag="OO330_002424"
     CDS             5657..6646
                     /locus_tag="OO330_002424"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883258.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            6746..6949
                     /locus_tag="OO330_002425"
     CDS             6746..6949
                     /locus_tag="OO330_002425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883259.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7051..7287
                     /locus_tag="OO330_002426"
     CDS             7051..7287
                     /locus_tag="OO330_002426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883260.1"
                     /translation="METHALSDDCLDCVYDWYAEKNGQFFSATSPEELLGLVAMWEVR
                     GNDWRANLNEINTYQKAIEESPFFDKNGNEIESD"
     gene            complement(7353..7585)
                     /locus_tag="OO330_002427"
                     /pseudo
     CDS             complement(7353..7585)
                     /locus_tag="OO330_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(7695..8465)
                     /gene="yafV"
                     /locus_tag="OO330_002428"
     CDS             complement(7695..8465)
                     /gene="yafV"
                     /locus_tag="OO330_002428"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5883261.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            8646..9092
                     /gene="ivy"
                     /locus_tag="OO330_002429"
     CDS             8646..9092
                     /gene="ivy"
                     /locus_tag="OO330_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5883262.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9135..11579)
                     /gene="fadE"
                     /locus_tag="OO330_002430"
     CDS             complement(9135..11579)
                     /gene="fadE"
                     /locus_tag="OO330_002430"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5883263.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            11819..12397
                     /gene="lpcA"
                     /locus_tag="OO330_002431"
     CDS             11819..12397
                     /gene="lpcA"
                     /locus_tag="OO330_002431"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5883264.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            12603..13370
                     /locus_tag="OO330_002432"
     CDS             12603..13370
                     /locus_tag="OO330_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5883265.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13341..14081)
                     /locus_tag="OO330_002433"
     CDS             complement(13341..14081)
                     /locus_tag="OO330_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5883266.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            14465..14986
                     /locus_tag="OO330_002434"
                     /pseudo
     CDS             14465..14986
                     /locus_tag="OO330_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15162..15659
                     /gene="rayT"
                     /locus_tag="OO330_002435"
     CDS             15162..15659
                     /gene="rayT"
                     /locus_tag="OO330_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5883267.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(15876..17588)
                     /locus_tag="OO330_002436"
                     /pseudo
     CDS             complement(15876..17588)
                     /locus_tag="OO330_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            17560..18345
                     /gene="lafU"
                     /locus_tag="OO330_002437"
     CDS             17560..18345
                     /gene="lafU"
                     /locus_tag="OO330_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5883268.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            18416..19471
                     /gene="dinB"
                     /locus_tag="OO330_002438"
     CDS             18416..19471
                     /gene="dinB"
                     /locus_tag="OO330_002438"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5883269.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            19523..19816
                     /gene="yafN"
                     /locus_tag="OO330_002439"
     CDS             19523..19816
                     /gene="yafN"
                     /locus_tag="OO330_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5883270.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            19819..20217
                     /gene="yafO"
                     /locus_tag="OO330_002440"
     CDS             19819..20217
                     /gene="yafO"
                     /locus_tag="OO330_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5883271.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            20227..20679
                     /locus_tag="OO330_002441"
     CDS             20227..20679
                     /locus_tag="OO330_002441"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5883272.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLTKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            20925..21968
                     /locus_tag="OO330_002442"
     CDS             20925..21968
                     /locus_tag="OO330_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5883273.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            22030..22644
                     /locus_tag="OO330_002443"
                     /pseudo
     CDS             22030..22644
                     /locus_tag="OO330_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(22701..24158)
                     /gene="pepD"
                     /locus_tag="OO330_002444"
     CDS             complement(22701..24158)
                     /gene="pepD"
                     /locus_tag="OO330_002444"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5883274.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            24420..24878
                     /gene="gpt"
                     /locus_tag="OO330_002445"
     CDS             24420..24878
                     /gene="gpt"
                     /locus_tag="OO330_002445"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5883275.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            24970..26214
                     /gene="frsA"
                     /locus_tag="OO330_002446"
     CDS             24970..26214
                     /gene="frsA"
                     /locus_tag="OO330_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5883276.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            26272..26673
                     /gene="crl"
                     /locus_tag="OO330_002447"
     CDS             26272..26673
                     /gene="crl"
                     /locus_tag="OO330_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5883277.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(26712..27767)
                     /gene="phoE"
                     /locus_tag="OO330_002448"
     CDS             complement(26712..27767)
                     /gene="phoE"
                     /locus_tag="OO330_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5883278.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         6810 a         7045 c         7466 g         6557 t
ORIGIN      
        1 caatggagag tgattactta tattttttgg ccaccagggg ctagatttta atccggtatt
       61 aaaggagcaa atatcatgag ttttgtatct acgggtaata aaccagttgg aaacggtgga
      121 cccgtcatca ccacaccacc aatagctggc gaaagtggcg gtatgagcac aggttcagct
      181 gtaaccgatg tatctggtgc cgccgaggag atggcagaac aggctgcggc ggacctgttt
      241 ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag tagctgctgc gcaggctgcc
      301 gctgcagcag cagggatctc ggatatggct ggtgcagttc aggatgcagc ggcgtcgctt
      361 gcagccggtg ctccgggggc gcataacgtc acagtaagtg gtagtgcggt gccgccgcag
      421 atgcttttgt ttgcaggcat gaatggttcg gaagggcttg ggaatctgtt ttcttacaca
      481 gtacagctta aaacgctgga cgccctgaac ctggggtacg tttcccccgc tgctaacctg
      541 ccgcttcagc tcatggtggg caaagacctg tgcgtcagta tcgaactgga cggcggcgga
      601 aaacggtata tcagcgggct ggtgacggcg gcgagggtgg tcggccatga gggccgttcg
      661 gtaacgtatg aattacgcat tgagccatgg ctgaagctgc tgacccatac cagcgactac
      721 aaagcgttcc agaacaaaac cgtggtggat attctcgatg aagtactgga tgaatattcc
      781 tttccggtgg aaaagcggct ggtggaaaat tatccacccc gcgcctggca ggtgcagtat
      841 ggtgaaaccg acttcgattt catccagcgc ctgatgcagg aatgggggat ttactggtgg
      901 tttgagcaca gcgagaacag ccacacgctg gtgctggtgg atgccatcaa tggtcataaa
      961 gcgtgccctg actcgccgct ggtggagtgg caccaggaag ggctgaaact ggataaggaa
     1021 tttatccaca ccattacggc cagtgagagg ctgcgtactg gaaagtgggt gatggacgat
     1081 tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg tggccagtcc gcgtgatacc
     1141 ggtcatgctg aatacgagca ttacgaatgg cctggtgatt acttcactac gggcgaaggc
     1201 gagatgctga cgcgcattcg tatggaagcg cagcgcagcc caggcagccg ggcacatggg
     1261 gccggtcata tccgtaccct catgaccggg tacaccttta cgttgatgaa tcacccgacg
     1321 gcagaaatta atcaggaata tctgctggta cagaccactc tgtttttacg ggataatgcg
     1381 cagcacagcg gtcaggatca gcattttacc tatgtcacca cgtttgaact gcaccccacc
     1441 agcgaagttt accgcccaca acgaaccctg agcaaacccc acaccaaagg cccgcagagc
     1501 gccatcgtca ccggcccggc ggggcaggaa atctggacgg ataaatatgg ccgggtgaag
     1561 gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa acagttcctg ctggatccgc
     1621 gtcagctatc cgtgggcggg taaaggattc ggcatgatcc agatcccgcg tattggccag
     1681 gaagtactgg tggatttcaa aaacggcgat ccggatttgc ccatcatcgt ggggcgcacc
     1741 tataaccagg acaccatgcc gccgtggggg ctgccgggca tggcgtcgca gagtgggata
     1801 ttcagccact cattgcaggg agggccgact aacggaaata tgctgcgctt cgatgataaa
     1861 acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc tcaataccac ggtaaaaaac
     1921 aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca ttgtcaaaaa tgaaaccatc
     1981 agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg atggtctttc cgtcaaactg
     2041 gctcaaacca tcaatgtcgg aacgacatat cgtctggatg ttggcgatca atttaccctt
     2101 cgttgcggca atgcagcgct tgtattacac aaggatggct cgattgagtt ttgcggaaaa
     2161 caactgttac tgcacaccag cgatgttatg caactcattg gcagtggcat tgatatgaat
     2221 ccggatggcg gcacagccat gactgcggat gatattgccc cgttacctac ccccgacgtt
     2281 tctgaataaa aaatataaaa ggaatttttc tatgaccagc ctttaccgta ttcaggaggg
     2341 ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc aatatcttcg tcccatcggg
     2401 caatgaaagg gcaactccca gtctgaatat ttcccgcgat acgctcaggc cagatgaaaa
     2461 tctgaccacc tatattgacc gccagatagc cttgatgaaa aaaaaaacct aggccagcac
     2521 cgggtacttt cgcggggaac ggcactggcg ggaactggcg aagctgccat gacaggtgaa
     2581 cagattctcg caactcatcg ttctggaaaa acagaagtgt accagcgtca ggccgggttt
     2641 atcaccgggc cagcaaaggt actaatgttg acgctgacca ctcagcgtcc ttttgatgat
     2701 cacaccgacc agctatggac tgaatggttg actagttttc agcctgctaa aagctaaaaa
     2761 aaacagggag gttttgtcat gtatgaagcc gcacgggtgg atgaccccat ctaccacacc
     2821 agcgcccttg ccgggttttt gataggggcc atcataggta tcgccattat tgccgtcgcc
     2881 agctttgctt tttttacctg cggctttttg gccgggctga ttttgagttt tctggctgat
     2941 cagatagctt ccggagtcct gcaactgggt gaggctattg ggcgctcgat acattccaca
     3001 gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca acagtcgccc ggccgcccgg
     3061 gccgtactga gtacggtgat atgcgaagac cattccccgg aaatccgcat tgcccaaggt
     3121 tccggcaaca tctacatcaa cagccagctg cccgtaagga tgaccacact gagtgtgatg
     3181 cagtgattga ggacggctcc cccaatgtct ttctcggcgg cggtacgcag acggtactgg
     3241 aaatcagccc ggaaatccct gactggctgc gtcaggtggt ggatgtgctg tatgtggtcg
     3301 ccggaatgct tggcgggctg gctggcgcct ggagtcaggc ggcaaagatg gggtcgaaat
     3361 ttggtacaaa atgtgcggca aaatttattg gtggcgaaat ggtcgggatg gccatctctg
     3421 acacagtcat gggattgttc agcaatccgg tagatgtcac cactgggcaa aaaattttgc
     3481 tgccggagac ggattttacc ctgccaggac gcctgcctgt cacctgttcg cgcttttacg
     3541 ccagccacct ggaaactgaa ggattgctgg gcagaggatg gcggctgaac tgggagataa
     3601 acctgcgtga ggatgaaaca tatatcacct tcatcggtgt acagggtcgg gaactgagtt
     3661 atccgaaaga gatgctgata ccgggccacc agatattcga cccggaagag cagttttacc
     3721 tgagccgtct gcatgacgga tgctatgtgc tgcattacac cgaccgcagc tattacgtgt
     3781 ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt tatggagacg ccgtaccggc
     3841 agcgtattgc tttcgggcgt gagaacggca ggctggtgcg ggtggcctcc agcagcggac
     3901 accatctgtt attgcaccgc acgatgacgc aagcagggga acgcctgtcg catattgaac
     3961 ttgtgaaggg cgggcgtccc ggtaacctgg tggaatatcg ttacgacgat aacgggcaac
     4021 tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca gtttgcctat gaaaatggcc
     4081 tgatgacaga gcatcgcaac gcgacgggct ttacctgtac ctaccactgg gaagagattg
     4141 aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg cgaggattac cggttccact
     4201 atgattttgc tggcgggcag acggtggtaa ccggcaggcc ggagcagaag tggcagtggt
     4261 ggtttgatga agagacgtat gtgactgcgc accggacgcc gggcggcgga atgtaccgct
     4321 tcacgtacaa cgaaaaccat tttccggtag cggtggaact ccccggcgaa cgcagggtaa
     4381 cgctggaata cgacaccctg agccgggtgg tgaaagagac ggacccggca ggccgggtga
     4441 cacagacgca gtggaacggc agttttgctg aaatcacgcg ccgggcgctg gatgacgacc
     4501 acgtatggaa ggcggattac aacgaacacg gccaggtgat acgggagacg gacccggaag
     4561 ggcgcgtcac gcggtacggg tatgatgaac aggggctggc ggtcagccgc acggatgcgc
     4621 ggggcggcga ggttgcgctg gcgcacgatg cgcgcggtca actgaggcgt tacaccgact
     4681 gttcagggtg cgccacgggt tatgaatacg atgaagacgg caacctgacg gcggtgacgg
     4741 acgcggaggg gaaaacggta cgcatcagct ataaccgtca ggggctgccg gagacggtct
     4801 gccaccccgg taaacagcag gaccgttaca cctggaacgc gctggggtta ctgagcagcc
     4861 accgtcgcat cacgggcagt gtgcagtcct ggcagtatac gccgcgcggg atgctggcgc
     4921 gccatacgga tgaagagaaa cgggagacgc gctggcagta caccccggaa gggctggttg
     4981 ccagtctgag caacggcaac ggggctcagt accggttcag ctacgacggc gacggacggc
     5041 tgacgggaga acagcgcccg gacggcctga taaggatgtt tgcgctgaat gcggacgggt
     5101 ttccggtcat catcccgacg cagggcacag agggcggggt gcgcaatgaa cagcaggagc
     5161 gtgatgcgct gggccgcctg ctgcgcagtg acacgcagca cagtacacgg accttcagct
     5221 acaaccgtct tgaccagata acggaagtga cgctcacccc cacggaagaa ggggaacggc
     5281 tgcaccacat gcaggcggac acggtgcgtt ttgcgtatga ccgcagcggg tggctgacgg
     5341 cagagcacag cgtgcacggc agtatcaaat accggcgcga tgcgctgggc aatcccaccg
     5401 atatcacgtt gccggacggg cagcatctca gtcatcttta ctacggcagc gggcatctgc
     5461 tccagacggc actggacggt atcacggtca gtgaatatga gcgtgacagc ctgcaccgtc
     5521 aggtgatacg cacgcagggg aaactggcga cgttcagcgg ctacaacgcc gacaaccggt
     5581 taagctggca gcgcagcctg ccaggtggca gccagaatcc acagcaggcg gtactccccc
     5641 ggcgcagaac gacagcgtga cggcgcggga ctatatctgg aacgcagacg gtgaagtcgg
     5701 cggcatcaat gacaaactgc gcggttgcct ggtgttcagc tatgaccgca gcggctggct
     5761 gaccagccgc acggggcaga tgtatgacca tgaccattat tattacgaca aagcgggcaa
     5821 cctgctgacg gatgagtatc agggagcggt gatggataac cgcctgccgg gttacgggcg
     5881 tgaccgctac cgctataacg aatggggcga actgacagag cgcagggacc agcagctgga
     5941 gtggaacgcg cagggccagt taacgcgggt catcagcagc aacagcgaga cgcggtatca
     6001 gtacgatgcg ctgggcaggc gcatcagcaa ggcgaccagc aatctccata ccgaccgggg
     6061 agaacgaagc cggacaacgt ttgtgtggga agggttccgg ctgttacagg agacaacgtg
     6121 gcagggaaag cggacatacc tgtacgatgc ggaacagccg tacacgccgg tggctgccat
     6181 cacggggcgc ggagagagcc agaaaatctg gtattaccac acggacctga cgggcacggt
     6241 acacgaggta accgcgccgg acgggacgct ggtgtgggcg ggctaccagg cgggatttgg
     6301 tgaaaaccgg ggcgacatca gcaacagcgg ggcgtacttt gagcagccgc agcggctgcc
     6361 ggggcagtac tttgacgaag aaacagggct gcattacaat ctgttcaggt attatgcgcc
     6421 ggagtgcggg aggttcgtaa gccaggaccc gataggatta aacggtgggt tgaacttata
     6481 tgcttatgcg cctaatccgt taggttggat cgatccgcta ggactgaatt ctttaggaat
     6541 agattcaaat aatatattta gaggagatgg taactataaa ggtggcggaa ttggacagcc
     6601 aaagagtggc acaatatcag cacaggacat tattgatcat ctctaagaaa aaggtgatgg
     6661 gcgtttaaca tcttttagta ctaaaaaaac atctagttcc aactctcgtg gtgtagattt
     6721 tttctcaaaa aaccatgttt tcaaagtggc agtttctgat ctcgatgatt tagttaaaaa
     6781 taatcagatc actgttttaa cacaagaaaa agtaaaagca atatttggtt tagaatcaag
     6841 caagaaaatt agaaaagaac ttggtaatat tcttcagaat atggctagga ataatgaggt
     6901 gttagttcat gggcaaattc ttggaaataa agttagccga tgtaattagt tagtggagag
     6961 gaagttgtgg acgaaattag aataagaatt gctggtaata cggctgcgcc ttgtttccat
     7021 gtgctaaaaa ataagggata aaaagtaaca atggaaactc atgcattatc tgacgattgt
     7081 ttagattgtg tatatgattg gtatgcggaa aaaaatggac aattttttag tgcaacgagt
     7141 ccagaagagt tacttggatt agttgcaatg tgggaagttc gtggtaatga ctggagagct
     7201 aatttgaatg agataaatac ttatcaaaaa gccatagaag aatccccgtt ttttgataaa
     7261 aacggcaatg aaatagagtc tgactaaatt ttattgatat agcaacgata ataaccggaa
     7321 atttcttcca gatctttccg gctttttatt atttaacttt cgttcagcac actaaacatc
     7381 ccgtctcgca tccccttaac tggctgttta acctgataaa gttccctgcg cagttcctgt
     7441 acttcgttgt tatcccaagc aggatcaaac atccctccga gatcttcacg gtaacgcccg
     7501 tgccgatctc aaatcccgct tttctcagcc atttggcgct gattttgatc accggcgtgt
     7561 cgctgcggtt gggcagataa cccacggtac agcgacgctg tgttttggaa atggtgactt
     7621 ctggcgtaat attgtgttta agttaggggc tttcgtaatc tagcccagcc cccggcccta
     7681 aacacaaccc ccactcacca caacctaaac tcatccgcat cccgccatgc cggaaacttt
     7741 tctctatatt cccgcaatgc caccatcgac agctccgcat caatacgcgt tgccttatgc
     7801 gcgtcggcgg tagcgataat ctcgccttgc ggattaatca cccggctgtc accgcgataa
     7861 tggcagccgt tgccatcgct gccgacgcga ttgcatcccg ccacatacgc ctgattctca
     7921 atcgcgcggg ccgtcagcaa tgcctgccag tgcagagagc gcggagctgg ccagttggcg
     7981 acgtacaggg cgaggtcata atcgttgaga ttgcgcgacc acactggaaa acgtaagtcg
     8041 tagcacacca gcggcaaaat acgccagccg cgccattcca caatcactcg cgcattgccc
     8101 gctttataat gcagatgctc atctgccatg cggaacagat gacgcttatc ataaaaatgt
     8161 accgtgccgc ccggctcaac cagcaaaaag cggttaaccg aaccagactc cgtttgtaat
     8221 gcaacactgc ctgcaatcag cgcattgcac tgctgcgcct tagctgtcat ccagttcact
     8281 acgtcatctt gtgcaagcga cgaagctgcc gcttccatgg caaagccgct ggtaaacatc
     8341 tccggtagaa cgatcacatc gcgcccggta ataccttcca gttgacgatc aaaatggcgc
     8401 aggttggcag gaccatccac ccacaccaac ggttgctgca aaagcgtaat cttcaaacta
     8461 ggcacggtgt acaactcctt tatgcgaagg gttttataac tttaacacct tatcaggcag
     8521 ttgccttagc gcagaataaa ttgataacaa atgctgatat tggaaatatc tgatttgcaa
     8581 attatcgtgt tatcgccagg ctttaggagg ttaataacat gggcaggata agctcgggag
     8641 gaatgatgtt taaggcaata acgacagtcg ccgcactggt catcgccacc agtgcaatgg
     8701 cgcaggatga tttaaccatt agcagccttg caaagggcga aaccaccaaa gctgcgttta
     8761 atcagatggt gcaagggcat aagctacctg catgggtgat gaaaggcggt acttatactc
     8821 ccgcacaaac cgtaacgttg ggagatgaga cgtatcaggt gatgagcgcg tgcaaaccgc
     8881 atgactgtgg ctcgcaacgt atcgctgtga tgtggtccga gaaatctaat cagatgacgg
     8941 ggctgttctc ggctattgat gagaaaacgt cgcaagagaa actcacctgg ctgaatgtga
     9001 acgatgcgct ttcgattgat ggtaaaacgg tgctgttcgc ggcgttgacc ggcagcctgg
     9061 aaaaccatcc ggatggcttt aattttaaat aattagcgga taaagaaacg gagcctttcg
     9121 gctccgttat tcaattacgc ggcttcaact ttccgcactt tctccggcaa ctttaccggc
     9181 ttcgtcgcca gctcttccgg atcaaagtca tcaacgttaa tactgcgcag acggctttct
     9241 tccgctttca ccagaatagc ggcttcatct ttatcaatca gccccttcgt cagcgcgttg
     9301 tgcgccagtt catccagacg ggtaaatggc agatttttac ccagctcttt acagatccgc
     9361 tgatgaatcg ggtcggcggc aatcacatcc accaacgcct cttccagtaa accaaccggg
     9421 ttatgctcgc ttggcgtcag gtactgaccg cgaccaatac gtgaacgagt ggcgttcggc
     9481 acttgtaaaa tcttcgccac tttatgatcc agcttgtcag acggtgccag ataatgacgt
     9541 ccggtcggga agatcaccac attcagcagc ccggcaacca cgcggttcgg gaagttttgc
     9601 agtaaattgt ccattgcctg ttctgcctga tacagcgcat cctgtacgcc ccagtgcacc
     9661 agcggcagat cggcttcatt acggccttcg tcgtcataac gcttcagaac ggcagaggcg
     9721 aggtaaagct ggcttaaaat atcccccaga cgtgccgaga tacgctcacg acgtttcagg
     9781 ctgccgccca gcagtgccat cgagacatca gaaagcaggg cgaggttggc gctcaggcgg
     9841 ttcaggtgct gatagtagcg tttagtggca tcgccggttg gcgtgctgct ggttaaaccg
     9901 cgcgtcaggc ccagccagaa gctgcgaact ttgttgctac cgacgtgacc gatatgtttg
     9961 aacaacagtt tatcgaacgc gttgacgtca ttgttcttcg ccgcttccat ctcttccagc
    10021 acgtacggat ggcaacgaat cgctccttgt ccgaagatca tcatgctgcg ggtcagaatg
    10081 ttagcccctt caacggtgat ggcaatcggt gcgccctggt aagcacgcgc caggaagttg
    10141 ctttgcccga gcataatgcc tttaccgccg gtaatatcca tcgcatcaat aatcgactgc
    10201 tgcccgcggt gggtacagtg atacttaacg atagccgaca gcacggcagg tttttcgccg
    10261 agcataatgc cgtaggtaat cagcgatgcc gcagcatcca ttacataagc gttcccggca
    10321 atacgcgcca gcggctcttc aatcccttcc atcttaccga tagagatttt gaactgacgg
    10381 cgaatgtgag aatacgcgcc cgttgccagc gctaccgatt tcacgccgcc ggttgagttg
    10441 gaaggcaggg tgatgccgcg gcctaccgag aggcactcca ccagcatccg ccagccttgc
    10501 ccggccattt tcggcccgcc gatgatgtaa tcgatcggca cgaagacatc tttaccgcgc
    10561 gtcggtccgt tctggaacgg tacgttcagc gggaagtggc gacgaccaat ttccacgccc
    10621 ggcgtggtgg ttgggatcag cgcacaggta atgcctaaat cttctgcacc gccgagtaat
    10681 ttttccgggt cggagagttt aaacgccagc ccaagcacgg tcgcaatcgg tgccagcgta
    10741 atgtagcgtt tgttccaggt cagacgcatc cccagcacct gctggccctg ccattcgccc
    10801 atgcagacaa tcccggtgtc cggaatcgcg cccgcatcgg aacccgcttc cgggctggtc
    10861 agtgcaaagc aggggatctc ctgaccacgc gccagacgcg gcagatagtg atctttctgc
    10921 tcgtcagtgc cgtaatgttg caacagttcg cccgggccta atgagtttgg cacgccgacg
    10981 gtaatcgcca ggatcccgct cacgccagag agtttttgca gcacacgaga ctgggcataa
    11041 gctgagaact ccagtccgcc gtactctttt ttgataatca tcgcgaagaa gcgatgctct
    11101 ttaaggtacg cccacaactc cggcggcaga tccgccagct catgagtaat ctggaaatca
    11161 ttcgccatcc ggcaggcttc ttctaccggg ccgtcgagaa acgcttgctc ttcagcggtc
    11221 aggcgcggct gcggatagtt atgcagcttt ttccagtccg gcttgccctg gaacaagtcg
    11281 ccctcccacc aggtggtgcc cgcatcaatc gcttctttct cagtgcgcga catcggcggc
    11341 atcaccttac ggaaaccgcg aaataccggc gcggaaatca tcgacttacg cataggcgca
    11401 aagttaaatg gcacgaggat aatggtcaga ggcaccagta cccacgccga ccacagacca
    11461 gcaacgccga gggcggctgt ccaggcgagc aaaatcagac tgctgataaa taagctcacg
    11521 cggtgataga acaacgcgcc gagcaggaca accgtagcga gaatactcaa aatcatcata
    11581 acgaaaagcc ccttacttgt aggaggtctg accacttgtg atgatatggt tgtagtggat
    11641 gtaaaaacat ttagcaatgt gtttacaata taattacaac aaagctcaca ttgttgctgt
    11701 ttttatccgc acttcaggtc aaaaagtcct ggtcatagca cctgcccgta cttctcgctt
    11761 ttggcggtat ccggtacact gcattttgtc tattacattt atgctgaagg atatcctcat
    11821 gtaccaggat cttattcgta acgaactgaa cgaagcggcg gaaacgctgg ctaacttttt
    11881 aaaagatgac gccaatattc acgccattca gcgcgcggcg gtcctgttag cagacagctt
    11941 taaagccggt ggcaaagtgc tttcctgcgg caacggcggt tcccattgcg acgctatgca
    12001 ctttgccgaa gagttgaccg gtcgctaccg tgaaaaccgt ccgggctacc cggcgattgc
    12061 tatttctgac gttagtcata tttcctgcgt cggtaatgat ttcggtttca atgatatttt
    12121 ctcccgctac gttgaagcgg taggtcgcga aggcgatgta ctgctgggga tctccacctc
    12181 cggtaactct gcaaacgtga tcaaagcgat cgcagcggcg cgtgagaagg ggatgaaagt
    12241 gatcaccctg accggtaaag acggcggtaa aatggctggc acggcggata tcgaaattcg
    12301 cgttccgcac tttggttatg ccgaccgcat tcaggagatt cacattaaag tgatccatat
    12361 cctgatccag ttaattgaaa aagagatggt taagtaagtc tggcgtaggc cggataaggc
    12421 gtttacgccg catccggcac ttgtgcgctg atgcctgatg cgatgctgac gcgtcttatc
    12481 atgcctacaa atctgtaccc gaaccgtagg ccgaataatt cgtttacgcc gcatccgacc
    12541 tgaaaattct taaatcaatc ttcgccgggg tccatgcgct cccgctgttg tggaggttac
    12601 ccatgtgcga attgctcggg atgagcgcca acgtccctac cgatatctgc tttagtttca
    12661 ccggacttgt acagcgtggt ggtggaaccg ggccacataa agatggctgg ggcattacct
    12721 tttacgaagg taaaggctgt cgcacattta aagatccaca acccagcttt aattccccca
    12781 tcgccaaact tgtccaggac tacccgataa aatcctgttc ggtggtggct catattcgcc
    12841 aggctaatcg gggcgaggtg gcgctggaaa atactcaccc atttacccgc gagttatggg
    12901 ggcgtaactg gacttatgcc cataacggac aactgacggg ctacaaatca ctggaaaccg
    12961 gcaacttccg cccggtcggt gaaaccgaca gcgaaaaagc cttttgctgg ctgctgcata
    13021 aattaacgca gcgttacccg cgcacgccgg gcaacatggc ggcagtgttt aaatatatcg
    13081 cctcactggc ggatgaactg cgggcgaagg gcgttttcaa catgctgctt tcggacgggc
    13141 gctatgtaat ggcgtattgc tcgactaatt tacactggat cacccgccgc gcgccgtttg
    13201 gcgtggcaac gttgctggat caggatgtgg aaatcgactt cagctcgcag accacaccga
    13261 atgatgtggt cacggtgatt gcgacacagc cgctgacggg caatgaaacc tggcaaaaaa
    13321 ttatgccagg cgaatggcgc ttattttgcc tcggggagcg tgtagtttga tgccagttgt
    13381 ggctgcacca cttcgtggct taacggcttg ctgaccacgt aacggccatt ggccacagaa
    13441 acggtcggtg gcttacgggt ttgctcaaag tagtcgtagc ccggcttcag ctgctcccag
    13501 aagtccttaa agttggaata tttatggcgc ttcatattgg cgtcggtcat gcggaatggg
    13561 taaatactca cctgcacgct cggctgacca aacaccagcg caccagtaac gaactggaag
    13621 atctcatcaa taccctgatt ggtcattgcg tagcagccga tggatacaca atcgccgtgg
    13681 atcatcaggt atttcccttc ataaccatgc gcacggtcat aagcattggg gaatccaata
    13741 ttaatcgctt tgtagtaacg gctgtctggt tttaactgat tacgctggac gctataaaac
    13801 ccttccgggc ttttgaaatc gccctgacgc tgttttggcc ctaagccgcc ggaatattta
    13861 cagattttat aactgtcgag cagttgatat tgctcgccca ttttgacgta aagatcgagc
    13921 gtacgttctt ccttgaagat ctggatgtag acaggggatc ccatcaactg ctgcttatac
    13981 tctttgctca ctggcgtggt cgagctactg ctgcccagca aaccggcaaa cgaaacgcac
    14041 gggatcaaca acatcgcaag aattaatgcg attttacgca tactgcttat tccttgataa
    14101 aacggttaca cacgccagga cggcaaaatg gatcccaaat cggaatagtc tggatttgga
    14161 aggctcacat tatcaccaaa agagttttac gcaagcctgt cggcgctggg tttacaaatt
    14221 tagcacaggg gcagttttca atagtccgaa aagcgcatgt atcatttatt tcctaatatt
    14281 atgaaatctc aatagtaata tgcaaataag caattaataa tcccgtttac tcctccggtc
    14341 ttttaaaatt ctcaccttca ttcagccggt ttatgtcttt catgtcttcg ttcttgctcg
    14401 gcagattttt gcatccaggc gtgttttcgt tatgcgtttt gctcccttta ctcgccagcg
    14461 ccaccacaac cgggagcaga taaaacagca tccagaatgg tttcctgcgc cgctcaaggc
    14521 gagtgacaga cgctggcagg cgctggcgga aaacaaccac tttttaagca gcgatcacct
    14581 gcataacatc accgaagtgg cgattcaccg cctggagcag cagcttggca agccttacgt
    14641 ctggggcggc acgcggcccg atcaaggctt cgattgtagt gggttagttt tttatgccta
    14701 caacaagatc cttgaggcga aactcccgcg cacggccaat gagatgtacc actaccgtcg
    14761 ggcaacgatt gtggcgaata acgacctgcg ccggggagat ttgctatttt tccatatcca
    14821 cagccgcgag attgccgacc atatgggggt gtatctgggc gacggtcagt ttattgagtc
    14881 actgcgcacc ggggaaacca ttcgggtaag ccggttagcc gaacctttct ggaaggacca
    14941 ttttttaggc gcgcgcagga ttttgacgga agagatgatt ttgtaggccg gataaggcgt
    15001 ttacgccgca tccggcggtt gtgcacggat gcctgatgcg acgctgacgc gtcttatcat
    15061 gcctacaaat ctgtgcgttt ttgcgagccg ctttcccgat ataacacagc gtcattgtcg
    15121 gcctttcctt ttcctcttac cgttgagaaa aaggagtcgc tatgtcggaa tatcgtcgtt
    15181 attacatcaa ggggggaaca tggtttttca cggtaaattt acgcaatcgt cgaagccatc
    15241 ttctgaccac ccagttccag acgctccgta acgccattat taacgttaag cgagacagac
    15301 cttttgaaat taacgcctgg gtcgttttgc cggagcatat gcattgcata tggacattac
    15361 ccgaaagcga tgatgatttt tcctctcgct ggcgggagat taaaaagcag ttcacccatg
    15421 cttgcggatt gaaaaatatc tggcaaccgc gtttttggga gcatgccatt cgcaatacaa
    15481 aagattatcg ccatcatgtt gattatattt atataaatcc agagaaacat ggctgggtaa
    15541 agcaggtgag tgattggcca ttctcaacgt tccatcgcga tgtcgcgcga gggttatatc
    15601 ccatcgattg ggcgggagac ataacggatt taagtgccgg ggagcgtatc attttataac
    15661 tgtgcgctgg tgccggatgc gcttacgctt atccggccta caaatcgtgc tttaacggta
    15721 ggccggataa ggcgttcacg ccgcatccgg cagttatgcg cagatgcctg atgcgacgct
    15781 ggcgcgtctt atcatgccta aaaacttgtg cctgaacggt aggccggata aggcgtttac
    15841 gccgcatccg gcaattgtgc accgatgccg gattcttaca tcaacgcccc cataatcttc
    15901 aactccagct catccggcac ttcgttatac gacagcacat gcagccccgg cgcaaacaac
    15961 cttgcataac gcgccagcaa agggcgtagc tgcggcggca ccagcagcac cggatctttc
    16021 cccgccgctt tcatctgctc tttcacctgt ggcatcgtgc tctggaactg gttgagcata
    16081 ttcgggtcaa ccggcacgct gtcgagcatc actttgccgg cctgttgcgc ctgattcact
    16141 acgttggtca gcagattttc cagctcatta ttcagcgtat acaccgtcag ctcctgcttg
    16201 cgaacgaacg gatgagtaat gctgcgccgc agcgccaggc gcacatcggc cgccagcaga
    16261 atatgatctt tagtcaccgt gctactggcg accagcacgg tggcgatggt gacgatatcg
    16321 cgcagggaaa cgccttcggt cagcagcgcc cggtacactt tcagcaattg gctgtaattg
    16381 agcgccgcgc ttaaatcctc cgccaggcgc ggtgccatcg acgacaaacg gttatgcaac
    16441 tgcgtgatgt catcatagtt aaacaaatca ggaatatagc tgcgcacaat cttgttcaca
    16501 tgggtggcaa tcacactggc gctgtcgatc acctgatacc ccatattcag agccttcgct
    16561 ttctgcgcag gctggatcca ggtgaccggt atcccgtacg ccgggtcgtt ccccagcacg
    16621 ccgtcaatct cgccgtaggt ttcgctggag ggcagcgcca tcagtttatc cgccggaata
    16681 tccgcttcat cagcctcaat gccgttgatg aaaatagcgt actggctggg cttgaggcgg
    16741 aagttttccc gaatgcggat ctccggcagc agcacgccgt taccgtcgga aatcacctgc
    16801 cgaacgccgc gaatccgctg ggtgagcggg ttgccctgcg ctttgtccac cagcgccacc
    16861 agcttgtagc cgaggcttaa gctgatgggc tcgatcagcg gaatagtttc ccaactgacc
    16921 tgttgttcgc tggtttcagt gatggtgcgg gtcagcgttt cgaggctttt ctcttccgcc
    16981 tccgccgcct gcgggcgttt gctcatccgc cagccggtaa agccaagcag ggcgctgaac
    17041 agcaaaaacg gcagatgcgg catccccggc accaccgcca gcacgaacat gatcccggtg
    17101 gcggtgtaga gcaccgacgg gcttgccagc aactggtggc gcacgtcatg ggcgatatcg
    17161 ccgttgtcgc tgacgcgggt gacgataatc gccgccgcgg tggagagcag cagggaaggg
    17221 atctgcgcca ccaggccgtc gccgatggtc atcagcacgt attgctggaa ggcggcgtcg
    17281 gcgctcaggt tgtatttgaa gatgccgata cagacgccgc cgctcaggtt gatcgccaga
    17341 atcatcatcc cggcgatggc gtctccgcgt acaaacttcg atgccccgtc catcgcgccg
    17401 tagaaatcgg cctcgctggc aacatcttta cgccgggttt gcgcctgcgc ctggttgatc
    17461 aatccggcgt taagatcggc gtcaatcgcc atctgtttgc cgggcatcgc gtccagggta
    17521 aagcgggcag aaacctcgga aatacgctcg gcctctttgg tgacgacgat aaagttgatt
    17581 gtcaacagcg tcagtaaatc cgagcgtgaa agcattatcg ccgcgctgca cgggcagtcg
    17641 atttttaacg gtggtgggct gtcgccgtta aataaaatca gcccgtctca tccgccaaaa
    17701 cctgccaccg tggcggtgcc ggaagagacg gagaaaaaag cgcgtgatgt gaatgagaaa
    17761 acggcgctgc tgaagaagaa atccgccacc gagcttggcg agttggcaac cagtatcaac
    17821 accattgccc gtgatgcgca tatggaagcg aatctggaga tggagattgt tccccaggga
    17881 ttacgcgtgc tgattaaaga cgaccagaat cgcaatatgt tcgaacgcgg cagcgcgcag
    17941 attatgccgt tctttaaaac tttgctggtg gagctcgcgc cagtgttcga ctcgctctat
    18001 aacaaaatta ttattaccgg gcataccgat gcgatggcct acaaaaacaa tatctacaac
    18061 aactggaacc tttcgggcga tcgcgcgctt tcagctcgtc gggtgctgga agaggccgga
    18121 atgccggaaa ataaagtgat gcaggtaagc gcaatggcgg accagatgct gctggacgcg
    18181 aagaatccgc aaagcgcggg caatcggcgt attgagatta tggtgctgac caaaagtgcg
    18241 tccgatacgc tgtatcaata ctttggtcag catggggata aagtggtgca gccgctggtg
    18301 caaaagctgg ataagcagca ggtgctttcg cagcgaatgc gctaaatgct gaatctttac
    18361 gcatttctca aaacctgaaa tcactgtata ctttaccagt attgagaggt gagcaatgcg
    18421 taaaatcatt catgtggata tggactgctt tttcgcagcg gtggagatgc gcgacaatcc
    18481 cgctctgcgc gatatcccta ttgctattgg cggcagccgc gaacgtcggg gggtgatcag
    18541 caccgccaat tatcccgcgc gtaaatttgg cgtacgtagc gctatgccta ctgggatggc
    18601 gctcaaatta tgcccacatc tcaccttgct tccggggcgc tttgacgcct acaaagaagc
    18661 ctcaaatcat atccgtgaaa tcttctcgcg ctacacctcg cgcattgaac cgttgtcact
    18721 ggatgaggct tatctcgatg tcaccgatag cgtccattgc cacggttctg cgaccctcat
    18781 cgcccaggaa atccgccaga cgatcttcaa cgagctgcaa ctgacggcat ctgcgggcgt
    18841 ggcaccagta aagtttctcg ccaaaatcgc ctccgacatg aataaaccca acggtcagtt
    18901 tgtgattacg ccggcagaag ttccggcatt tttacaaacc ttaccactgg aaaaaatccc
    18961 cggcgtcggc aaagtctcgg cggcaaaact ggaagcgatg gggctgcgga cctgcggtga
    19021 tgtacaaaag tgtgatctgg tgattctgct taaacgcttt ggcaaatttg gccgcatttt
    19081 gtgggagcgt agtcagggga ttgacgagcg cgacgttaac agcgaacggt tacgaaaatc
    19141 cgtcggcgtg gaacgcacga tggcggaaga tattcatcac tggtctgaat gtgaagcgat
    19201 tatcgagcgg ctgtatccgg aacttgaacg ccgtctggca aaggtaaaac ctgatttact
    19261 gattgctcgc cagggggtga aattaaagtt cgacgatttt cagcagacca cccaggagca
    19321 cgtctggccg cggctgaata aagctgactt aatcgccacc gcgcgtaaaa cctgggatga
    19381 acgccgcggc gggcgcggtg tgcgtctggt ggggctgcat gtgacgttgc ttgacccgca
    19441 aatggaaaga caactggtgc tgggattatg atgtatacta ttatgtatat tctggtgtgc
    19501 attattatga gggtatcact gtatgcatcg aattctcgct gaaaaatcgg tcaatatcac
    19561 tgagttacgt aaaaacccag ctaaatactt tattgatcaa ccagttgcgg ttctttctaa
    19621 taatcgcccc gcaggatatc tcttaagtgc cagcgcattc gaagcgttaa tggacatgct
    19681 tgctgaacaa gaggagaaaa agcccataaa ggcgcgcttt cgtccaagtg ctgcaagatt
    19741 agaggaaatt acacgccgcg ctaaacaata tcttaatgat atgacggatg atgatttcaa
    19801 tgactttaag gaataaggat gcgggtattc aaaacaaaac ttattcgcct gcaacttaca
    19861 gcagaggaac ttgatgcgtt aacggcggat tttatttcct ataagcgtga cggtgttttg
    19921 ccagatatat ttggtcgcga tgcactctac gacgactcgt ttacctggcc attaatcaaa
    19981 tttgagcgag ttgctcatat tcatctggca aatgtgaata atccatttcc gccacagttg
    20041 cgccaattca gcagaacgaa tgacgaagcg catttggtat attgtcaggg tgcgtttgat
    20101 gagcaagcat ggttgctcat tgccattctg aagcctgaac ctcataaact ggctcgagat
    20161 aacaaccaaa tgcataaaat tggaaaaatg gcagaagcgt ttcgcatgcg tttttgaatt
    20221 tatattatga ataacataca aataagagat tatcagcctg gcgattttca gcaactatgc
    20281 gctattttca ttagagcggt tacgatgacc gtcagtcagc attattcact acaacaaatt
    20341 gccgcctggg cgcagattga cgaatctcgc tggaaggaga aactcacgaa atcacaagtg
    20401 cgggttgcag ttattaatgc aaaactggtc ggctttatta cctgcgttga acactatatc
    20461 gatatgttat ttgttgaccc tgaatacacc cgccgtgggg ttgccagcgc tttgttaaaa
    20521 ccttttatta agtctgaatc cgaacttacg gtggacgcaa gcataaccgc aaaacccttt
    20581 tttgaacgtt atggttttca gacagttaag cagcagcgcg ttgaatgccg gggagcgtgg
    20641 tttattaatt tttatatgcg ttataaaccg caacattaaa tccagcttgc aatgaaaata
    20701 acgcccgcct agtatgtgcg cgttttctta ttccacaaac tgcaaggagg taaatcatga
    20761 caaatccttt atcaatgact ctttgcagac ctttccagga ttaattcttt ttttcttgcc
    20821 ctggattcgt ctgccatttc ctgatttttt aattgttact ctcaataatt caagtcgcag
    20881 cacatgcaac ttgaagtatg acgagtctat ttacggaatg gtatatgggc aattatattc
    20941 gtcccttatc cgatgcggta tttaccatcg catctgatga cctgtggatc gagagtttag
    21001 cgatccaaca attacacacc acggcaaatt tacccaacat gcagcgcgta gtggggatgc
    21061 cagatttaca ccccggacgc ggctacccga ttggcgcagc gttcttctcc gcaggccgtt
    21121 tttacccagc actggtcggc aatgatatcg gctgtggcat ggcgctatgg caaacagata
    21181 ttctcgctcg caaatacaac gccgataagt ttgaaaagcg attatcagct ttggatgacg
    21241 ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc atttgcacag catccgtggc
    21301 gcagctcgct tggctctatc ggtggtggca atcatttcgc tgaactgcaa caagttgatc
    21361 aaatcatcaa cgctgaactg tttgcactgg caggtctgga tgcgcagcat ctgcaactgc
    21421 tggttcatag cggctcgagg gggctagggc agtctatttt acagcggcat attgcctcgt
    21481 tttcgcatca tggtttgcct gaaggcagtg acgacgcgct ggcgtttgcg cgttttaatc
    21541 gccacctgat cgctttgcgc ataatgcaac aggttaaggc caccggaagt ccggttctgg
    21601 atgtggcgca taactttgtt agcgcgtgtc gaatcggtga tcaacaaggg gtcttacatc
    21661 gtaaaggtgc cacgcctgat gactgtggtc tggtggttat tcccggttca cgcggtgatt
    21721 actccaggct ggttcagccc gtcaggagtg aggaaacatt gcattcgctg gcgcatgggg
    21781 ctgggcgtaa atgggggcgc accgagtgta aagggcgtct ggcagcgaaa tacacagcga
    21841 cgcaactctc gcgtactgaa cttggcagtc gggtaatttg tcgcgataaa caactcatct
    21901 tcgaagaagc gccacaagcc tataaatcgg ctgaaagtgt ggtgcaatgt ctgggcaggc
    21961 cggattaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa acagtggagg
    22021 gaaaaaagga tgatcttgct ataactctcc tcagcgcagg ggccggaaga atgttgtctc
    22081 gcagtgaaaa aggcgctgga caggctaatt aaagaagctg cccgacaaga cgtcgcggta
    22141 acggtgctgg aaacagaaac aggccgctac tctgacacgc tgcgttcggc gctgatttct
    22201 ctggatggcg acaacgcatg ggcgttaagc gaaagctggt gtggcactat tcagtggatt
    22261 tgcccgagcc cgtatcgccc acatcacggg cgtaaaaact ggtttctggg cattgggcgt
    22321 tttaccgctg atgagcagga gcaatccgat gcgatccggt atgagacgct gcgttcgtcg
    22381 gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac gcatttggca
    22441 tccggtatta gcgtgaaggt tcagtcagag cgcagccagc atgctaacaa acggctggcg
    22501 cgattgctga ttgcctggaa gctggatcag cagcaatagg aaaatagcgc ggtgctgaaa
    22561 tcacagcggc gaatgttcca tcaccagatt gaacgtggca acccgcgacg aacgtttaca
    22621 gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca
    22681 tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagcagt
    22741 gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg
    22801 atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat
    22861 tccaggcccg cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt
    22921 tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct
    22981 ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag
    23041 tctttaccgc tgtcgatcag tgaacggatc aggcagtgaa tttctacgtt attgtcagtc
    23101 atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac gtcggaattg
    23161 cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca
    23221 atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt tttctctttc
    23281 tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt cagtgcgtcg
    23341 actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt
    23401 gtgccgccgt tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag
    23461 cgcaccagca gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg
    23521 cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga
    23581 tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct
    23641 tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagccg
    23701 aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga
    23761 accacgtttt cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca
    23821 cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct
    23881 ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt
    23941 aagacgaccg gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta
    24001 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
    24061 gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc
    24121 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
    24181 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
    24241 ttgcgagata tgtattgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
    24301 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
    24361 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
    24421 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
    24481 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg
    24541 taccgggggc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
    24601 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
    24661 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgct gttgcgattc
    24721 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
    24781 tggttgatga ctatgttgtt gatatcccgc aagatacctg gatcgaacag ccgtgggata
    24841 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcattgc
    24901 cgggcgttgt tctttttaac ttcaggcagg ttacaatagt ttccagtaag tattctggag
    24961 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
    25021 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
    25081 gctcttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
    25141 tggcgcggta ttgacccacg tgaaatcctc gacgtccagg cacgtattgt gatgagcgat
    25201 gctgaacgta ccgacgatga tttatacgat accgtgattg gctaccgtgg tggcaactgg
    25261 atttatgagt gggccaccca ggcgatggtg tggcaacaaa aagcctgtac ggaagaagat
    25321 ccgcaactca gtggtcgaca ctggctgcat gcggctacgt tgtacaacat tgccgcctat
    25381 cctcatctga aaggagatga cctggccgag caagcgcagg ctttatcaaa ccgcgcctat
    25441 gaagaggccg cccagcgtct gccgggcacg atgcggcaga tggagtttac cgtacccggc
    25501 ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt cccgacagta
    25561 ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta tgaacgttat
    25621 tttgcgccgc gcggcattgc gatgctgact attgatatgc cgtcggtggg cttttcttca
    25681 aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgtcttaaa ggcgctgcct
    25741 aacgtaccgt gggtggatca cactcgcgtc gcggcctttg gtttccgttt cggcgctaac
    25801 gttgccgtgc gtctggcata ccttgaatcg ccgcgtctga aagcggttgc ctgtcttggt
    25861 ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat
    25921 cttgacgttc tggcgagtcg tttggggatg catgatgctt ccgacgaagc gttacgcgtg
    25981 gagctgaatc gctattcatt aaaagtgcaa ggattgctgg ggcgtcgctg cccaacgcca
    26041 atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc acgcttaatc
    26101 acctcatcat ctgctgacgg taaattatta gagatcccat ttaacccggt gtatcggaat
    26161 tttgacaaag gtcttcagga aatcaccggc tggatcgaaa aacgcttgtg ttaaaaattt
    26221 gctaaatttt gctaatttgg taaaacagtt gcatcacaac aggagatagc aatgacgtta
    26281 ccgagtggac acccgaagag cagattgatc aaaaaattta ccgcactagg cccgtatatt
    26341 cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt atgcgtcaac
    26401 gtgaaaccgg caccggaagt gcgtgaattc tggggctggt ggatggagct ggaagcgcag
    26461 gaatcccgtt ttacctacag ctaccagttt ggtctgttcg ataaagcagg cgactggaag
    26521 agtgttccgg taaaagatac tgaagtggtt gaacgactgg agcacaccct gcgtgagttt
    26581 cacgagaagc tgcgtgaact gctgacgacg ctgaatctga agctggaacc ggcgaatgat
    26641 tttcgtgatg aaccggtgaa gttaacggcg tgagtgaaat gtgccggatg catcacatcc
    26701 ggcaatattc attaaaactg atacgtcatc ccaaccgcga cagtatcatc attattaata
    26761 ttcagcttat tatcgctatc cagttggttg attttataat caacaaacgc tgacatattt
    26821 ttgttgaaat aatacgtagc accgacgtcg atataattga ccagatcttc atcaccgatg
    26881 ccctcaatat ctttcccttt cgataagaca taacccagcg atggacgcag accaaagtca
    26941 aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc gccagttatt
    27001 ggcgtcattt tgcgtgtttc agaatagaaa gttgccagat aaatattatt ggcatcgtat
    27061 ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc cacggctttg caggttctgc
    27121 tcgttggtgc gatctgagtt ggtataggcc ccactaatgg cgaaatcgct gccgccaaag
    27181 tcatatgtca atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc gcggttttcg
    27241 tttttccctt gatattgcag ggttaagttc aggccatcga taacgccgaa gaagtcggtg
    27301 ttccgatacg tcgccagacc gctggcgcgt ttggtcataa agttgtcggt ctgcgccgag
    27361 gaatcgccac caaattccgg gaacatatcg gtccaggctt ccacgtcata caacgcgccc
    27421 aggttacgac cataatcgaa agaacccaaa tctttatatt tcaacccggc aaaagcgaga
    27481 cgcgtttttt gctgtgcagt atcactctcc gctttatttc cggcaaactc cgcttcccac
    27541 cgaccataac cagtcagttg atcgttaatt tgagtttcac ctttaaaacc aaaacggata
    27601 taactctggt cgccatcttt actggcgtta tcactcatat aatgcatggc tttaactttg
    27661 ccatagacat ccagtttatt accgtcttta ttatatattt ctgcagcctg tacagatgca
    27721 gatgccacaa tgcccatcac cactaatgcc agagtgctct ttttcatttt cattcctgat
    27781 tttaattaac gcgcgaatat tcagcgggag agccccgttg aaaacaggaa agtttttaac
    27841 ctgagattgt taaagatata ttacagatta atgatatt
//
LOCUS       DAJTRB010000056        27601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTRB010000056 DAJTRB010000000
VERSION     DAJTRB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.056"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO330_002449"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO330_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5883279.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO330_002450"
     CDS             complement(1614..1937)
                     /locus_tag="OO330_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5883280.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO330_002451"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO330_002451"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5883281.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO330_002452"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO330_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5883282.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO330_002453"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO330_002453"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5883283.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO330_002454"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO330_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5883284.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO330_002455"
     CDS             7537..8574
                     /locus_tag="OO330_002455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5883285.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO330_002456"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO330_002456"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5883286.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO330_002457"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO330_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5883287.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO330_002458"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO330_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5883288.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO330_002459"
     CDS             10745..11626
                     /locus_tag="OO330_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5883289.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO330_002460"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO330_002460"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5883290.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO330_002461"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO330_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5883291.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO330_002462"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO330_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO330_002463"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO330_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5883292.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO330_002464"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO330_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5883293.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO330_002465"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO330_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5883294.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO330_002466"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO330_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5883295.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO330_002467"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO330_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5883296.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO330_002468"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO330_002468"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5883297.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO330_002469"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO330_002469"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5883298.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO330_002470"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO330_002470"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5883299.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO330_002471"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO330_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5883300.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO330_002472"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO330_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5883301.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO330_002473"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO330_002473"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5883302.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO330_002474"
     CDS             24893..25273
                     /locus_tag="OO330_002474"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5883303.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO330_002475"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO330_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5883304.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO330_002476"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO330_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5883305.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="OO330_002477"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="OO330_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6808 a         6867 c         7237 g         6689 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcttgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgga ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctggggcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggtgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc ctggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAJTRB010000057        27418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTRB010000057 DAJTRB010000000
VERSION     DAJTRB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.057"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..357
                     /locus_tag="OO330_002478"
     CDS             <1..357
                     /locus_tag="OO330_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5883306.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="OO330_002479"
     CDS             619..981
                     /locus_tag="OO330_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5883307.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1892
                     /locus_tag="OO330_002480"
     CDS             978..1892
                     /locus_tag="OO330_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5883308.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRMTAEWFYKLHNKISNPKIEENVGDFRLMS
                     RGVVENIKLLPERNLFMKGVLSWVGGSTDVVEYARAERVAGSTKFNGWKLWNLALEGI
                     TSFSTFPLRMWTYIGLFVAGLAFLYGAWMIIDTLAFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYTEVKQRPRYLLKEK"
     gene            1894..3207
                     /locus_tag="OO330_002481"
     CDS             1894..3207
                     /locus_tag="OO330_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883309.1"
                     /translation="MAERIRYVFLAIFCALLCKESIDIWMMNNGDFDRAITPFMNGIR
                     QFSHDGTLLYTLKENFSSIVNYEYKSSFSYILYLYAYVISIFTHTFDLRLWSSLSKIV
                     YICALYKLFVEINNKKYSYLLFPVSALLLFTPSILSQFTAFYQEQIVITLLPVITYII
                     LKKEISRFDKVLLVACAALIATSKSQFFYIPILISLISIIFIDIRDKKFHLAMVIATI
                     IALSFSFFSPSATKYNSYHSLYFGTLLYNKSNGKENPSWAVDECIGVDAWGNKFDLDK
                     GAVTTKDAGACFAKNKERGLKDSLLLMLKQPSMFLLLPFDSGVRTQLTEDYFHVFKEN
                     KLIISKSVFLNEVQKIKDSALSTVRIPVAFIAMIASLIFIRKKYSVAIFTLSAFSISQ
                     FYISFIGEGYRDLDKHLFAMNFSFDLMIFIVLSIILDKVASGIYK"
     gene            complement(3242..3721)
                     /locus_tag="OO330_002482"
     CDS             complement(3242..3721)
                     /locus_tag="OO330_002482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883310.1"
                     /translation="MTLEMRGNTCLGVGYTASASRPDVSISGYSANEQAIFIDYDNLW
                     IPSQSPVVDATVKWKSSPDFAFGVSVVGATGAMGNRRNILSVTRGATGVYTVNANHDI
                     ANVAVEITTVGVPGYGIIGGYPTANSFVVRMYNTAGAPTDIDFIALGKDYRARFNAT"
     gene            3880..5026
                     /locus_tag="OO330_002483"
     CDS             join(3880..4130,4129..5026)
                     /locus_tag="OO330_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5883311.1"
                     /translation="MSGKRYPEEFKIEAVKQVVDRGHSVSSVATRLGITTHSLYAWIK
                     AYGPDSSTNKVQSDAQAEIRRLQKELKRVTDERDILNLQRGVLRKAVRLRYAFIRDNT
                     HCWPVRLLCRVLDVHPSGFYAWLQQPDSRRHHADLRLTGLIKQFWLESGCVYGYRKIH
                     LDLRDTGQQCGVNRVWRLMKRAGIRAQVGYRSPRARKGETSIVTPNRLQRQFNSEAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKVIGWSMQPRMTKDIVLNALLMAVWRRNPQ
                     KQVLVHSDQGSQYTSYEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARGDIFDYIEMFYNSKRRHGSSDHMPPTEYENQYYQRLGSV"
     gene            5043..5558
                     /locus_tag="OO330_002484"
                     /pseudo
     CDS             5043..5558
                     /locus_tag="OO330_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(5530..5949)
                     /locus_tag="OO330_002485"
     CDS             complement(5530..5949)
                     /locus_tag="OO330_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5883312.1"
                     /translation="MSYFYSASTNGFYSTEFHGTNIPDDAVEISESEWKTLINSQSVT
                     KMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQDAVDLGMATDD
                     ETKLLLAWKKYRVLLMRVNTSTALDIEWPTPPAVQAR"
     gene            complement(5954..6604)
                     /locus_tag="OO330_002486"
     CDS             complement(5954..6604)
                     /locus_tag="OO330_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883313.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPM
                     TFSRVLAVTVTLNVTGAAGQPTVSATNVHNTGFNITVSPGSGYGASSDAYYIAMGY"
     gene            complement(6608..7192)
                     /locus_tag="OO330_002487"
     CDS             complement(6608..7192)
                     /locus_tag="OO330_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR5883314.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSARDPAIAGAAPSLTRVHQRAD
                     ALMRELDPHTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPVATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(7183..8241)
                     /locus_tag="OO330_002488"
     CDS             complement(7183..8241)
                     /locus_tag="OO330_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5883315.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSTGGVLRVPIACSSAGAVGNADDGTSLILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIAGSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKGSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            complement(8228..8653)
                     /locus_tag="OO330_002489"
     CDS             complement(8228..8653)
                     /locus_tag="OO330_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR5883316.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(8653..9201)
                     /locus_tag="OO330_002490"
     CDS             complement(8653..9201)
                     /locus_tag="OO330_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR5883317.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     IEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPKARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(9201..10280)
                     /locus_tag="OO330_002491"
     CDS             complement(9201..10280)
                     /locus_tag="OO330_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR5883318.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(10277..11653)
                     /locus_tag="OO330_002492"
                     /pseudo
     CDS             complement(10277..11653)
                     /locus_tag="OO330_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309364.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
     gene            complement(11678..13585)
                     /locus_tag="OO330_002493"
     CDS             complement(11678..13585)
                     /locus_tag="OO330_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5883319.1"
                     /translation="MADNFQLKAIITAVDRLSGPLKGMQRQLKGFQKEVSSLALGAAG
                     AGTAIMGALALPVKSAITLESKMADVRKVVDGLDTPDAFKAMTEQVRALSTELPMSAD
                     GIAEIVAAGGQAGIARDELMQFATDAVKMGVAFDTTAEESGQMMAQWRTAFNMTQDEV
                     AGLADKINYLGNTGPANAKKISDIVTRIGPLGGVAGVASGEIAAMGATIAGMGVESEI
                     AATGVKNFMLSLTAGNSATKSQKQALRFLRINPKKLAADMQKDARGTMLSVLDAMAKV
                     PKEKQAAVLNALFGKESLGAIAPLLTNLDLLRTNFRRVADSQQYGSSMQKEYASRAAT
                     TENQLLLLQNQLDAISSTLGETFLPEVNDGLEAVKPLLEEVRTFVRENPELVKTIAKI
                     GLALLTVGAAAGSLSRIMKVLGGVMNMTPAKGLIALLVGGAYLIIDNWETVGPVIKKV
                     WHVVDETAQVMGGWETVLKAIALFMATKWVADVTKSITAVTREMRTLGKVSAETGLMG
                     KGRGFIGKAGVYGFLGTLMYEPVKDTLESVVPEDTVNWLDNKGLFLASDWTPFFDRKE
                     YEQYQASLSQYKPNVPLLNPSSSMTQHSELKVTFENAPLGMKIIDVPGKADPLMKITH
                     DVGYSPFRFPR"
     gene            complement(13670..13993)
                     /locus_tag="OO330_002494"
     CDS             complement(13670..13993)
                     /locus_tag="OO330_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5883320.1"
                     /translation="MIELVLKKPIIAHKETLHVLEIREPTYDEIEALGFPFSVSPDGG
                     MKMDSQVALKYIPLLAGIPRSSAAQMTKLDIFKAGMIVMRFFTGLETEESSGSDSTMS
                     RGSGN"
     gene            complement(13990..14346)
                     /locus_tag="OO330_002495"
     CDS             complement(13990..14346)
                     /locus_tag="OO330_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5883321.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(14346..15842)
                     /locus_tag="OO330_002496"
     CDS             complement(14346..15842)
                     /locus_tag="OO330_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5883322.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGTGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATV
                     TLTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIKDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKDTQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVLERDASDPNRLNTLFPPDYVNQLRVFAVVNQLRLQYSEESA"
     gene            complement(15826..15996)
                     /locus_tag="OO330_002497"
     CDS             complement(15826..15996)
                     /locus_tag="OO330_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5883323.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(16005..16565)
                     /locus_tag="OO330_002498"
     CDS             complement(16005..16565)
                     /locus_tag="OO330_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883324.1"
                     /translation="MKLTPVIAALRARCLYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEIPLPS"
     gene            complement(16562..17086)
                     /locus_tag="OO330_002499"
     CDS             complement(16562..17086)
                     /locus_tag="OO330_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883325.1"
                     /translation="MDQTAFLHVDFKQPEEMEFNRARLRRAFVQIGRVYMRDARRLVM
                     RRGRSAPGENPGYQTGRLARSIGYYVPRKSSRRSGLMVRISPNQKNGQGNRRFPEGSA
                     YYPAFLYYGVRHAAYGMSKKDKRQKKQHSSRWRLAPRNNFMADVIDQRRYWTQKLLSR
                     ELQRSLRPVRRKKT"
     gene            complement(17058..17468)
                     /locus_tag="OO330_002500"
     CDS             complement(17058..17468)
                     /locus_tag="OO330_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5883326.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDRVYRVKRQR
                     DLNGARRFLLLECTELGAEEQMGGRSGSDSIFTR"
     gene            complement(17465..17788)
                     /locus_tag="OO330_002501"
     CDS             complement(17465..17788)
                     /locus_tag="OO330_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5883327.1"
                     /translation="MLLTMEEIKAQLRLDEDFDVDDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(17791..17991)
                     /locus_tag="OO330_002502"
     CDS             complement(17791..17991)
                     /locus_tag="OO330_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883328.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(18040..19245)
                     /locus_tag="OO330_002503"
     CDS             complement(18040..19245)
                     /locus_tag="OO330_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5883329.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGDATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAVA"
     gene            complement(19260..19910)
                     /locus_tag="OO330_002504"
     CDS             complement(19260..19910)
                     /locus_tag="OO330_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5883330.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            complement(19888..21129)
                     /locus_tag="OO330_002505"
     CDS             complement(19888..21129)
                     /locus_tag="OO330_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5883331.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRAAGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(21129..21311)
                     /locus_tag="OO330_002506"
     CDS             complement(21129..21311)
                     /locus_tag="OO330_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883332.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(21323..23056)
                     /locus_tag="OO330_002507"
     CDS             complement(21323..23056)
                     /locus_tag="OO330_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5883333.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFANMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDLVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(23053..23547)
                     /locus_tag="OO330_002508"
     CDS             complement(23053..23547)
                     /locus_tag="OO330_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5883334.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(23673..24023)
                     /locus_tag="OO330_002509"
     CDS             complement(23673..24023)
                     /locus_tag="OO330_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5883335.1"
                     /translation="MPPRTPKACRVRGCSHTTTDSSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDCIRARVLKRDKGLCQLCLRAGVVREAKTVDHIIPKARGGTDADSNLQSLC
                     WPCHKAKTAREWLK"
     gene            complement(24475..24867)
                     /locus_tag="OO330_002510"
     CDS             complement(24475..24867)
                     /locus_tag="OO330_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR5883336.1"
                     /translation="MKLSYKLAISAFFFTVIGSFIWSANHYYSKYQHEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRTQADIKVAVADDDCAVRPVP
                     AAAADRLRKFADSLRASSGYTVTGESDR"
     gene            complement(24851..25327)
                     /locus_tag="OO330_002511"
     CDS             complement(24851..25327)
                     /locus_tag="OO330_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5883337.1"
                     /translation="MQVLNSQRIAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFKEAGGVVNEVEL"
     gene            complement(25331..25657)
                     /locus_tag="OO330_002512"
     CDS             complement(25331..25657)
                     /locus_tag="OO330_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR5883338.1"
                     /translation="MPHNPNTWLELFQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            26019..27096
                     /locus_tag="OO330_002513"
                     /pseudo
     CDS             26019..27096
                     /locus_tag="OO330_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015700614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27115..>27418
                     /locus_tag="OO330_002514"
     CDS             27115..>27418
                     /locus_tag="OO330_002514"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883339.1"
                     /translation="MSFFKFIIRFLSAFAFGFAIFYFASRKIDLSGISTPWSLITLLA
                     FPFSYCIAAFFKVSEADENTSLSDNELRRLRPIIDAKKRHLGFLIFFYLIAAFSGAI"
BASE COUNT         6613 a         7459 c         6581 g         6765 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tctcaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccga ttctaattca aagcattacc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatc gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgatc
     1261 tgcaggaccc gattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgata
     1321 tggtactggc taagcgctca gaccgttcta cagacggaag actgaagcgc atgactgctg
     1381 agtggttcta taaacttcac aataaaataa gcaatccaaa gattgaggag aacgtcggcg
     1441 atttccgact aatgtctcgc ggagttgtgg aaaacattaa attattacca gaacgcaacc
     1501 tgttcatgaa aggagtattg agctgggttg gtgggagtac agatgttgtt gaatatgctc
     1561 gcgctgagcg tgttgctggc agcactaagt ttaatggctg gaagctctgg aacctggcgc
     1621 ttgaagggat taccagcttc tcaacatttc cgttacgcat gtggacgtac attggcctgt
     1681 tcgttgcagg cctggctttc ctgtacggtg cctggatgat cattgatacg cttgcattcg
     1741 ggaatcctgt gcgcggttat ccatcattgc tggtatcaat actttttctc ggtggtgtgc
     1801 agctcattgg tatcggagtt ttgggcgagt acattggccg gatttatacc gaagttaaac
     1861 agagacctcg ctacttgtta aaagagaaat aaaatggctg aaagaataag gtatgtattc
     1921 cttgcaattt tttgtgcatt actgtgcaag gagtcaattg acatatggat gatgaataat
     1981 ggcgacttcg atagggccat aacacctttc atgaatggga tcagacagtt ctcacatgat
     2041 ggcacattac tttataccct gaaggaaaat ttcagttcaa ttgtgaacta tgagtataaa
     2101 agctcattta gttatattct ttatttatac gcttatgtta tatcgatatt cacacacaca
     2161 tttgatctta ggttatggtc atctctttcc aaaatcgtgt atatttgtgc gttatataaa
     2221 ctatttgtag aaataaacaa taaaaaatat tcttatcttt tatttccggt atcagcatta
     2281 ttgctattta ctccttccat actttcacaa ttcacagcat tttatcagga gcaaatagta
     2341 atcactttac ttccggtaat tacttatata attcttaaaa aagaaatatc aaggtttgat
     2401 aaagtattac tagttgcatg cgcagcgcta atcgccacat caaaatctca gttcttctat
     2461 attcccattt taatttcttt aatttcaata atatttattg acataagaga taaaaaattt
     2521 cacttagcta tggtaattgc gacgataatt gcattgtcat tttcattttt ctcgccaagc
     2581 gcaactaaat ataacagcta tcactccctt tatttcggga ctttgctata caataaaagc
     2641 aatggaaaag aaaatccatc gtgggccgtg gacgaatgca ttggtgttga tgcatgggga
     2701 aataaatttg atttggataa gggagctgtt accacaaaag atgctggagc ctgcttcgca
     2761 aaaaataaag aaaggggatt gaaggattct ttgcttttga tgctaaagca accttccatg
     2821 ttcctccttc ttccttttga ttctggagtt cgaacccaat taactgaaga ttacttccat
     2881 gtcttcaagg aaaataagct aataataagt aagagtgttt ttttgaatga agttcaaaaa
     2941 ataaaagact ctgctctatc aactgtgagg attcctgttg catttatagc catgatagca
     3001 tcactgattt ttatccgaaa aaaatattct gttgcaatct ttacattatc tgcttttagc
     3061 atctctcagt tttatattag cttcattggc gaaggatata gagatttaga caagcatctt
     3121 tttgcaatga acttttcatt tgacctaatg atatttatag tactatcaat tatattggat
     3181 aaggtagcat caggaattta taagtaaaca tcccccccac agttatcctg ggggggggat
     3241 tttatgtagc gttaaatctt gctctatagt cttttcctaa tgctatgaaa tctatgtcag
     3301 tgggagcgcc tgcagtattg tacatcctta caacgaatga gtttgcggta ggataaccac
     3361 ctattattcc gtatcctggt acccctacag tagttatttc tacagccacg tttgctatat
     3421 catgatttgc atttactgta taaaccccag tcgctcccct tgtaacactg agtatatttc
     3481 tcctgtttcc cattgctcca gtcgcaccaa caacgctcac accaaaagcg aagtcaggag
     3541 agcttttcca tttaacagta gcatccacaa ccggtgattg tgatgggatc cataagttat
     3601 cataatcgat aaaaatagcc tgctcattgg cggaatatcc acttatacta acatctggcc
     3661 tcgatgcgct tgccgtatag ccaaccccaa ggcaggtatt accgcgcatc tctagtgtca
     3721 tcttaacccc ttccgggtaa gaataattac cgcccaatac tacaacctgg ctgacggggt
     3781 actggctgga attattgtta aaattagagt taataatgaa tcgccacagg tttaacagac
     3841 acctcagagt catttaagat gacttaaaga gaggtgccca tgagcggtaa gcgttatccc
     3901 gaagagttta aaattgaagc ggtaaaacag gttgttgatc gcggccattc tgtttccagc
     3961 gtggcaacac gtctcggtat caccacccac agtctttatg cctggataaa ggcatatggc
     4021 ccggattcct caaccaataa agtccagtca gacgctcagg ctgagatccg acgcctccag
     4081 aaagagctga agcgggttac cgacgaacgg gacatattga atctacagcg gcgtacttcg
     4141 caaagctgtc cgactgaggt acgcctttat ccgtgacaac acccattgct ggcctgtccg
     4201 actgctttgt cgggtgctgg atgtgcatcc gagtggtttt tacgcctggc ttcagcagcc
     4261 ggattcacgg cggcatcatg ctgacctgag gctgacgggg ctgataaagc agttctggct
     4321 ggagtcgggt tgcgtttatg gttatcgcaa gatccacctc gacctgcggg ataccggaca
     4381 acagtgcgga gttaaccgtg tctggcgact gatgaagcgt gccgggataa gggctcaggt
     4441 cgggtaccgt agcccacggg cacgtaaggg tgaaaccagc atcgtgacgc ccaacaggct
     4501 ccagcggcag ttcaactcgg aagcaccgga tgagcgttgg gtaacggaca tcacctacat
     4561 ccggactcac gaaggctggc tgtatctggc tgtggttgtt gacctgttct cgcgcaaagt
     4621 tattggctgg tcaatgcagc cccggatgac aaaggatatt gtcctgaatg cacttctgat
     4681 ggccgtgtgg cgacgtaatc ctcaaaagca ggtgctggtt cattctgatc aaggcagtca
     4741 gtacacaagc tatgagtggc agtcgttcct gaaatcacac gggctggagg gcagtatgag
     4801 ccgtcgcggt aactgtcatg acaacgcagt cgcagaaagt tttttccagc tactgaaacg
     4861 tgaacggata aagaaaaaga tctacggaac gcgagaagaa gctcgcggtg atatttttga
     4921 ttacatcgaa atgttttata acagtaagcg tcggcatggt tcgagtgatc atatgccacc
     4981 aactgaatac gaaaaccaat attatcagcg gctcggaagt gtctagatta tctgtggcga
     5041 ttcatatttc tgcatcaact gaatatcttg ctgtgggtgt cggtatccct gcatattcct
     5101 gtttagatgc tcctggctca tataaagctg gttatgcaat ttgccgttct gcagatttta
     5161 actcatggga atatgtgcca gaccatcgcg gtgaaaacgt ttatagcacc gaaacaggag
     5221 aatcgaaaga aatcacagct ctgggtgatt accctgaaaa tacaaccact atcgccccgt
     5281 taacgccata tgataaatgg gatggtgaga aatgggtgac ggatactgag gcacagcata
     5341 gcgccgcagt aggtgcagca gaagtacagc gccagtctct ggttgatgct gctatggctt
     5401 ccatcagtct gattcagctg aaattgcagg ccggacgtaa actgacgcag gcagaaacaa
     5461 cccgccttaa cgctgtgctg gattacattg acgcggtgac ggcaacagat accagcaccg
     5521 cgccggatgt catctggcct gaactgccgg aggcgtaggc cattcaatat cgagtgctgt
     5581 tgaagtatta acacgcatca atagcacgcg atattttttc catgccagta acagtttcgt
     5641 ttcatcatct gttgccatac ctaaatcaac cgcatcctga agcggcgcta ttacattcgt
     5701 tgcctctgct atcagtgcag atttcttttc atcatttatt aaggctaatt gttctggtgt
     5761 tggagaagga cggtcaacaa tgacaggatg accgttctca ccacaggtaa tcatttttgt
     5821 tacactctgt gagttaatca gtgttttcca ctctgattcc gagatttcca ctgcatcatc
     5881 aggaatattg gtgccgtgaa attcagtcga ataaaatccg tttgtcgatg cagaataaaa
     5941 atatgacatt tcgttaatat cccattgcaa tgtaatacgc atcagaagat gcaccatatc
     6001 ctgaaccggg cgacacagta atattaaatc cagtgttatg gacatttgtc gccgatacag
     6061 tcggctgccc agccgcgcca gtaacgttca atgtaactgt gacagcaagg actcttgaga
     6121 atgtcatcgg gaacctcatc ccgtatgttg aagatacatt aggggagcca ttcaactgac
     6181 cgcactgtaa aattaatcca gacggaaatt tttgcgcagc agccgttgcg gtattacttg
     6241 acgcgaacag gctcatatca ggtatctgat tcgcccctgt acctacattc cttttagctg
     6301 cttctcccaa accaaggttt tcgagagccg ttttcaccgt gccatccgat ttgatatcgc
     6361 caaacggatt cttgcggctt aacagcagcg cgcgaagtgc ggtaagcaac tgatcgtgcc
     6421 gccccttctc caggctggca ccggatgcct ccaccacgct gcaaagctcc tcctgcaaca
     6481 tgtcaaagta gtcatcatcc agatcggtgg caggcgtgcc agtctggggg ttaccacggg
     6541 taaaaccgtt cttacccgcg ccgaacttat ccttctgcgc ggttttcgtg tctatacgat
     6601 gcatggatta ctccggatat ttaaaaatca cataggtatg cgacgggcag agtttgttaa
     6661 gcacacactc gacaacggtg tccccccaga agcgcagcgc ggaatcacag ggatcgccac
     6721 atgtcatcca ggtggtgttg gtggcgactg gcatgttgac ctgccagtaa taccgccatt
     6781 ccggcgcatt caccgcgtca gtacaggcag atgagcaggt gaacgtgctt ttgtcgtatc
     6841 gcgtgatggt agcgtctggt ctgcccaggg cagcaagctg tgcaaggtaa aaatcctcat
     6901 tgatgccgcc cgccaggtta accttcgcat ccagtcgttg ctgacgctgg cgaagggtct
     6961 gtgtccctgc gggaatacat tcatccggca gaccgcacag acgctcccag cggtttatca
     7021 gttcagtggt ggtgtgcgga tccagctccc gcatcagggc atccgcacgc tgatgaacgc
     7081 gggttaatga cggtgccgca ccggcaatcg ccggatctct ggctgaccac gccggaccgg
     7141 gtggcaacag tgccgacaac agacggatgt aatcatcgtt tgtcacgtcc atgaaatcgt
     7201 ccccagtacc gccagttcat tttttgcaat ggagatattg tccgccggtg caagcaactg
     7261 atggctgtat tccccgttcg caccggaaat cgcttcactg atacgtgacc ccttcagttc
     7321 tccctgcgga taaccatcac gcagcaggaa cgaacgcaac tcggcggtaa tggcagcccg
     7381 tatttccggt gtgtccggcg tcacacggat atgaaaatcc actttatgcg ccaccggcct
     7441 gaatacatac aaatcagagc ctgccaccgg ggccagtggc ccgatatgtt gtcttgccgc
     7501 cgtttccgtt gattcttccg gaatgggatt aatcaggtca ctgccggcaa tcatcacacc
     7561 gacagtcccc gttcccatcc agtgtcggta tgtccatgcg cgggtaatgc cgggcacttc
     7621 tttagcccag acgacatagt ccccgtcagc cccgccctga ggcgtccagt aataccgctc
     7681 aatgacgcgg gcgcgccacg tttccagctc ttcagtatca aatccacctg tcagggtatc
     7741 tgccacgccg gaagacggca gaccattcac cggcgtgacc aggattaatg acgtaccgtc
     7801 gtcagcgtta ccgaccgcgc ctgcacttga gcaggcgatc ggcacgcgca ggacaccacc
     7861 ggtgctggtt gcatcggcag ttgccgtgta ctgcaccagg tcatcgcgct gaataacact
     7921 cccggcggtc accttcaggc catcgctgac accttcccag cgcatatacc cgctggcagc
     7981 cgtggccccc ttgcgcggac accgtttcat cgcagcatgt cgcgccagcc aggactcatc
     8041 gcacaggtca ggcagcatgt tcattgccag ataatcgatg taaccgtaaa ccgtatgcag
     8101 cgccgccgca tacacctttg cccgcacgtc ttcatccatg cgccggagcg tgtcgctgac
     8161 gtccagcctg gcgaataaat cgttacggag catactgata ttttctgcca gcgtcgggcg
     8221 ctgaaattca ctgtccgcca tgcgttatcg cactccacag atcatcaaaa gaaatcatta
     8281 ctggtccatc acgacgccag agggtgatac tgttacccag ctcattaatc ccggtgcggc
     8341 ggatatccag atcaatacgg gacaccacgc cgtcatcaat catccattgc aggcattcgc
     8401 ggatatatcc ccttaccgtc tgcaccagct gattggtcag tttgctgcgc tgaagcagcc
     8461 acagtcggga gccgtaacgg tcattctgta ccgcaggcca ggtatccccc caccatccca
     8521 tcgggacgtc ggcgttgtca tcaggctccg cccgccgcca ggtaaacagg gaaatcacca
     8581 cggcgcgggt cagcggatcc agcggtgcgc tggcgcaggt gcgtttaccg ttcaccgtca
     8641 gccacagttc catcatgcct ccatcgcttt atcaggtttg tcggtgttac tgccctgacc
     8701 gttctctctg tgacgatgcc cgttataggc aagccgcatc gctgacatgg tggtgccgcc
     8761 ggagtcgcac aggtctttca cctgtcctgt cacttccagg tccatttcaa aacgtgcctt
     8821 aggcgcattg cgaaacgtga tcgttttacc tgcaccgtcc accacgatcc cctcccgggt
     8881 cagcgtcaca gactgcccct gatcgtcata gacagccacc tcacccgtct gcagcccttt
     8941 caggcggtag cgccggtccg acaccgtaac aaccaccgca tgagaacggt cgccatccgg
     9001 aaacaacacc accgcttccg caccgctgtt tgcccttgcg gtaaaaccgt agggttcaag
     9061 atgttcaatc ccggctttgg gttcaccggc aatcagggac acatccacgg tctgacattt
     9121 cgtggcggca ctgatgcttt tcaccactgc ccgcccaatc aggccgagga gttgtcgctg
     9181 catggcttca atcgtcctca tcagaacggg tcctcctgta ctctggcttt tttctttttc
     9241 cgcgcgccgg gggcttcggg ttcaggcaga taagcatcag gcgggccgac acggatttcc
     9301 gtcagggtgc cgttctggtc ctgagtaaac gtgacttccg aaacaagcag ttcggtattg
     9361 tcgaaaccac agaccggatc aaagacaatc acccgctggt tgggctgcca cagcgtaccg
     9421 ttaccctgtc gccagccctg caccacatag gtggtttcat ccgtccgcgc cgcccgttgc
     9481 cgggcttcaa agtcagcacg cgcaatacag cctgcccccg tagcctgccc tgtctgcctg
     9541 atatacatcg gacggtaacg ggcaataaat gcgtcctctg tgcgggcccg cagcgcggtg
     9601 gtggtggcct caccgaaatc atcgtcgttt ccggcacgct gccccgccac ctggtaaaca
     9661 gaaaatcgct cccggatact cttctccgta tcgcaggaaa ggatgttttc cccgagtacc
     9721 agcgcagtat gtgcccgcgt tgagccaata ccgccaatca ccagcctgcc gtgcgggtcg
     9781 tcgtaagcca gtgcctgctg ctgaccgagt attttgttga ttacctcaat caccgtttca
     9841 ccgtgatcag gctgaacatc aggaataaca cccgacggcg caccgctgtt caccacctca
     9901 atgccgaaag gcgcagcaag cgcctgcgca atctgtacca gcgatcgtcc gttaaactgt
     9961 gtcggttcgg ctgcacagtc aatcaggtca gcggtcagac tgcgtccggc aataccggtg
    10021 ctgaccgaac gggcatcgta acgaacgggc gtcgcctcca cccagccggt gatcaccagc
    10081 tcatcaccaa tcagcacctc cacttttgaa ccgtttttaa tgcgcggctg aagcgtggta
    10141 ataccctcat ctcccggcca ctggcgggtg atctccacac tgaaatcccg cgccagccgt
    10201 tcaataccgg caccgatgcg caccgatgtc cagccattcc actcccggcc atttacccgt
    10261 agcgtgacat tgtcgttcat tgcactggca ccttcagagg gatcaccggc acaaagccgg
    10321 gatgcgtaat ggcattacgc cggataatgt ccgcgtcacg cgccgcgtta tcaaactagg
    10381 tcgccgccag caccagcgcg ggtaaaacct catccggtgt gcgctgaatg atccgtgcag
    10441 actgttcaag gcgcgtgttg atatccgcat tcagatctgc tttcacccgg cgcagcgcca
    10501 gaaacagcgc atcgctggtt gtacgggaca actccttatc aattgccgta ttcagtgtgt
    10561 cgcgaatgtc agtcagttct tcccacgtcg gcaggtcaac cgtgtttttc accgccggtg
    10621 cattgttcag tgccggatgc gtgacggaag gccagcaagt gctctgcgcg ggtgttgttg
    10681 cctgccccac tgcggcattc tgcatcaccg cggaagttgt tggcgcaggc aatcgggtga
    10741 cggcatacgc cgcttcgctg attgcggtcg tacgaagggt gctggcaacc acgttacgct
    10801 gctgcgtcgc cgtggcggtg gttttactgt ccgttttcca gacgccgcgg ggttgcagat
    10861 cgctgccgag gctgacaccg gaaagcgttt tgatcatggt gaccaggtcg ctggcgttac
    10921 cataaaggcg tttcccggta cgccacattt tctgcacctg ctcaacgaaa tttttgcctg
    10981 acgatggcgg cggcagaagt accgagatat ccccctgcaa cagcctggcg gcatccgata
    11041 cggcagaatc caccactttc atcgcatcag aaacataccc cagcattatg ctggcattac
    11101 cgataacgtc gttctgcacg aaatccgcca caccatcgat actgaaaccg ctgaagctgt
    11161 cactgatgca gtcatccagt gcagaacagg atgacatcag cgtctgcgcc gtcgccgcac
    11221 ctgatgtggg gtaagagagt tctcccgctt cgacaaactt caggtcaaag cggacaatac
    11281 gcccttcact cttcgatgtg ctgacccgaa cttctccgtc aacacagact ttcagctcac
    11341 cgtatgtcgg atggacaagc gtgccgggac cgggtttatt cagcgcgtca atcaggcgat
    11401 cgcgctggtc aaagcagtca tctcccacca cataagccgt gatggacggg cgaaaagtga
    11461 ttttccccag gtcttcggta tagggtttgt cgcggttcgg gtattcatgt gtttccacac
    11521 ggcgaccggt tcccgcactt tcttcttcaa ccttaaacgg cacgccgcga aatgacgcgt
    11581 cctgaagtct gtcacgccag cctgaagacg acgaaagtaa tgaaggtcgg gtgggaaatg
    11641 aggataaatc catagactga cctcaaaaag gactgcgtta tcgtggaaaa cgaaaagggg
    11701 aataccccac atcgtgcgtg attttcatca ggggatcggc tttgcccggt acatcaatta
    11761 tcttcatacc tagcggagca ttctcgaacg tgactttcag ctcgctgtgc tgtgtcatgg
    11821 aagaagatgg attcaacagc ggaacattgg gtttgtactg gctcaggctg gcctgatact
    11881 gctcgtactc tttacgatca aaaaaaggcg tccagtctga agccagaaac agccctttat
    11941 tatccagcca gttaaccgta tcttcaggaa caacactttc cagagtatct ttaaccggct
    12001 catacatcag ggttcccaaa aaaccatata ccccggcctt cccgataaag ccgcggcctt
    12061 tccccatcaa tcccgtttct gccgatacct tccccagcgt acgcatctct ctggtcactg
    12121 cggtaatgga tttggtaacg tcagcaaccc atttggttgc cataaacagg gcaatcgctt
    12181 tcagaacagt ttcccatccc cccatcacct gcgccgtttc atccaccacg tgccagactt
    12241 tttttatgac agggcctacg gtttcccaat tatcaataat gaggtaagcg ccaccaacca
    12301 gaagagcaat cagcccctta gcaggcgtca tattcatcac accgccgaga actttcatga
    12361 ttctggacaa agagcctgca gcggccccca ctgtcagtaa agccagaccg attttagcaa
    12421 tggtcttaac gagctccggg ttttcacgga caaacgttct cacttcctca aggagcggtt
    12481 ttaccgcttc aagaccatca ttaacctcag gaagaaacgt ttcccccagc gtggaagaaa
    12541 tggcatcaag ttgattttgc agaagtaaaa gctggttttc cgtcgtcgct gccctcgaag
    12601 catattcctt ctgcatcgaa ctgccatact gctgggaatc cgcaacccgc ctgaagttgg
    12661 tacgcaacaa atcaaggtta gtcagcagag gtgctatcgc gcccagagac tctttcccga
    12721 acagggcatt cagcacagct gcctgttttt ctttaggcac tttagccatc gcatccagta
    12781 cagacagcat ggtgccccgg gcatctttct gcatatcagc agctaatttc ttcggattga
    12841 tccgcagaaa acgcaatgcc tgtttctgcg attttgtcgc agaatttccc gcggtcaggg
    12901 aaagcatgaa gttcttgacc cctgtggcgg caatttctga ctccacgccc atcccggcaa
    12961 tggttgcccc cattgccgcg atttcgccgg aagccacacc tgcaacacca cctaaaggac
    13021 caatacgcgt aacaatatcg gagattttct tcgcgttcgc tgggccggta ttaccaaggt
    13081 agttgatttt gtcagccagc ccggccactt catcctgcgt catattaaac gcagtacgcc
    13141 actgggccat catctgcccg gactcttcag ccgtggtatc aaaggccacg cccatcttca
    13201 ccgcatcagt ggcaaactgc atcagttcat cacgtgcaat cccggcctga ccgccagccg
    13261 ccacaatttc cgcgatcccg tctgcagaca tgggaagctc agtagacaaa gcgcgtacct
    13321 gctccgtcat ggccttaaac gcatccggcg tatccagacc gtccaccact ttgcggacat
    13381 cagccatctt cgattcaagg gtgatggctg attttacagg gagtgccagt gcccccatta
    13441 ttgcagtacc cgccccggca gcgcccagag caaggctgga gacttctttc tgaaatccct
    13501 taagctgacg ctgcatacct ttaagcgggc cggacagcct gtcaacagcg gtgatgatgg
    13561 ctttcagctg aaaattatca gccatgcttc atctcctcat ttatacggac agcctctgcc
    13621 tccagatcag caaagtggga aatagccgtc cggcgaagtt caagggggtt taatttccag
    13681 aaccacgcga cattgtagaa tcgcttccgg agctctcttc cgtctccaag ccggtaaaaa
    13741 aacgcattac aatcatgcct gccttgaaaa tatccagctt cgtcatctgc gctgcagacg
    13801 agcgcgggat cccggccaga agcgggatat atttcagcgc cacctgactg tccattttca
    13861 taccaccatc aggcgaaaca gagaaaggga accccagcgc ctcaatctcg tcatacgtag
    13921 gctcacgtat ttccagcaca tgcagtgttt ctttgtgggc gatgatcggt tttttaagta
    13981 caagctcaat cactggtaat ccccttcttc accgtggaac tcaagatcaa ccgtgccttc
    14041 ttcggcatta tggttcgctt cgccgtgcag ccaggcagac gacaatacat agacctgacc
    14101 gttcgccagc tcggcagtga tggtcatctc atcagacgag gtgattttgc tcaccggaaa
    14161 attcttcggc accttgaagg tccctttgac ataaggcgca cggtgagttt ccttgcggtc
    14221 cactgaaccg tccaggccga tgatgtcatc attgaccgtc ctgttcatgg gcacctcaat
    14281 gccgccggtc agcgatagct gctgaccgtc aattttgaaa taacaggttc ccccgatacg
    14341 ggccattatg cagactcctc tgaatactga agacggagct ggttaaccac ggcaaagaca
    14401 cgcaactggt taacatagtc aggcgggaac agcgtgttca ggcggttcgg atcgctggca
    14461 tcacgctcca aaaccaggta ctgcttaaac agttcgtagt tttccacgat ccccgctcgc
    14521 tcaagctgac ggtaggttgc cagcagttcc cctttgatca ccgccggggt gacaatcgcc
    14581 tgaccgggac caaagcgggt accgtcactg gcaagcttgt gacgcccgta cttactggta
    14641 atgacggatt tcagtttgcg cagtacatac gcgctggtat gcagcgtctc gctgtcgagg
    14701 tagctgttat ccgcaacccc gtaagcgttt ttcctgtacg tggtgacatc acgctgaatg
    14761 cgcagtaccc cgctttcgac atacgccgtt gccacgccat gagacagcag ggtctgttgt
    14821 tcggtcatcg tgaaccgttt ccccttcggc gcaggcagca tacccaccag ctcaccggtc
    14881 tgcgtgggac gtgccggatc gttgcgaata aacaccgctg cgcgggcggt acggcttgcc
    14941 gccagctcat cggcaggcgt ctgggtgtct ttttcgtacc ccgccagggt aatgtgctgc
    15001 tggttaaact ggtcacctgc ggtcaccagt tctgacagcg tgccgatctt tgccgtatac
    15061 acatgaccat acagctgacg cgcatagctc cagcgaccgc tggtatcgtt catctcggtc
    15121 accagcgtgt taacggaggc cgtgtcgttg aacggcaggc cgatataatc aaacggctca
    15181 tccgccattg cagccaccgc gccggtgaga accggagcac ccgttccggc ggtacccgtc
    15241 gccacggcaa tctgtacgcc cgctggcagc acttcgcccc caccaaagcc gtagtaattg
    15301 aggctgacag gaatttcatt cccgcaaagc cccttatgac gcgcggtcag tgtgaccacg
    15361 cctgccgaag atgaagccgt aaacggcagg gccggaacgg cattgatggc atctttgata
    15421 ctgctggcaa tcgtcgtgac gttatcgccg ttggtcaccg gagcctgcac gcgggtacgt
    15481 cccacataga cattcaccgt gccgctttcg gttgcttccc cggtcaccgt cagcgtaacc
    15541 gttgccgccg cgcctgtggc ttccggaacg gcaatcacat acagctcacc aaacgggtcg
    15601 gtctggcgat aagcctcgac catacgcgcc agctgacttc ccgtaccaca aatctggcgt
    15661 gcatagtctg ccgacggcat cagcaccaga ctgttggcaa caatctctgc accgttattg
    15721 gcatgaccaa tcagcagcga tgctccgctg tcctgtgcag tattcgcagc ctggttatcc
    15781 atttccgcat aaaacagcgg aaccagcgta ttcgacggaa tggtgttaaa gcttatcgtc
    15841 atcggtgttc acctttttat tcacgcgccg gatatcaccc gctgcttcac ggcgcagcca
    15901 gtagttgttc tcgtcaacat ttcgcccttc ggcgggcaaa aggtcgccgc gggcagggtc
    15961 aggaactgac cgccctttaa caggtttgac aaacatgatg atcctcagga aggaaggggt
    16021 atttcggtgt gatgttcgat atcgccgtca ggcccgttac cgggatcgag ataatcaaca
    16081 tcaatcgcca gcgttcgcag ttcatccaga ctgttcaggt catcctgctg gcgggtatcg
    16141 tcttcggtca gctcgctgat gaccgaaaaa tcgaactgat aaatcagctc atgacgattc
    16201 agatccagca gcgtgccgcc gtcataggta atcgggttac cgcacgcttc cgggttccag
    16261 cccagcagag ccttaaagag catctgccgg acatcgtcca ccacatcata cgaggcaaac
    16321 tgaccgcgct catcacgccc gttactcagt atgacaacca cggagaagcc ctctttcagc
    16381 tcctgccagt agtcggtctg acttttgttt tctcccggag agtcatcacc cggtaccaca
    16441 tatgccgccg ggagtttcaa ctttccgacc tccggcagat ttttgaactg ggccgcgcct
    16501 gccacccggt tttcaaaata cagacagcgg gcacgcagcg cagcaataac aggagtcagt
    16561 ttcatgtttt tttccttctt acaggacgta atgaccgctg taactcacgg gacagtaact
    16621 tttgtgtcca gtaacgacgc tggtcgatga catcagccat aaagttatta cgtggtgcca
    16681 gccgccagcg ggatgaatgc tgctttttct ggcgcttatc ctttttgctc atcccgtatg
    16741 cggcatgacg cacaccgtaa tacagaaacg ccggataata cgcagaacct tcaggaaaac
    16801 gccggttacc ctgcccgttt ttctggttag gggaaatcct gaccatcagg ccagaacgac
    16861 gggagctttt acgggggacg taataaccta tagaacgcgc aagtcgtccg gtctgatagc
    16921 cggggttttc acctggagca gaccgaccac gtcgcatcac cagccgccgg gcatcacgca
    16981 tatagacacg cccgatttga acaaatgccc ttcggagcct ggcacggtta aactccatct
    17041 cctccggttg tttgaaatca acgtgtaaaa atgctgtctg atccactgcg tcctcccatt
    17101 tgttcttcgg cacccagttc cgtacactcc agcagcagaa agcgccgcgc cccgttcaga
    17161 tcacgctgac gtttcacccg gtacacacgg tcatcacaga ccacctcata atcagcagtg
    17221 atcccccggc ggtagcgaat ggtgatgtaa tgggtgatgg cgtccccggt ctgcgcggtt
    17281 tcctgccagg tggtggcact ggtctggata accttcgccc atgtccggaa cgtaaccggg
    17341 tattgaggct ccacgccaaa gttatccgcg ggcatatcca cccgctggcg gatcaggacg
    17401 cgtttattca gttcaccggg gtccggcaga atgtaggttg cgctggtctg cgcctgacga
    17461 attttcattg cggaaagtac ctgtacgggc cgacaagcca gccaaaactc tgcggcatgt
    17521 cgagtttctc cacttccgta accgacgagc ggttttcgta aaaatggctg ataagcatca
    17581 gcatccccag acgaatatca tccggcaggt gcagcccgtc cggatcgctg tccggaatgg
    17641 tttcatccgg tgcatagagc ttccggttca gatacgtttc cgtccgcttt tgtgccgcac
    17701 aggccagcag ttgcagatgg cggtcatcaa catcgaaatc ctcatccagc cggagttggg
    17761 ctttaatctc ttccattgtc agaagcatac tcagccctct ttactggtcg tggctttttt
    17821 ctcttttgtc gctttactgc tttttgcact ggttccgcgc tctgctaacc cggcctgaag
    17881 tgcaatctcc tgcacccggg caggaagcgc cccgtcgtca tactcaccgg cccgaatgac
    17941 ctcaacacgc ataccgtccg gtgaccattt cagatcttgt ttcaggatca tgattcttca
    18001 cccgtcagaa caggggcgcg gttccgcgcc cctgagtgat tacgccactg caatcttcag
    18061 cagtttgatg gcctgcgaat cgaccagcat gccgccggtg cgtttggtgg tataaaaacc
    18121 gacaaacggt ttattggtgt acgggtcacg cagaatgcgg gtgccgatac ggtcaacgat
    18181 ggtgtaaccc cgtttgaagt taccaaatgc aatggctttc gcatcagcgg cgatatccgg
    18241 catctgttcg ttttcagcga taccgtaacc cgccagagag gacggctgcc ccagctccag
    18301 ccccggacgc cacagatagt taccctcgct gtctttcagc agacggatgg caaacaggct
    18361 attgttgttc atcatgaact tcgcgcctgt gcggtgtgcc ttacgcagcg tgtaaatcag
    18421 tttgataatg gcgtctgcgg tcaccgccgt cgcgtcgccg gatacaatat gctgaagttt
    18481 gccgaacgcc cggaccttat cggtttcatc cgtggattca tacgccagga accctttcgg
    18541 cttcttggta ccatcgccgg tggtaaaggc aatttcttcc tgttcggcaa attcggttgc
    18601 cagctcgctg ttgatccatg cttccacgtt gaaaaaggca tcatccagca ttttctgggt
    18661 ggcctgcggg ttaccgtaga tttcccccat gaaaggttca atcaggccca gttttgaggt
    18721 ggcagtctgg gagcgcgcgt cagtctcgcc aacccatccg gaagccgtgc cgcccagatt
    18781 caccagtttt ttgtagtcgg aaccaccaac ggtgatcacc gtggcttcct ggcgcatcac
    18841 cacttcatct ttcagcaggg tgagaatgtt gcgatccagt gcttccggca cggcatagcc
    18901 gccgtcttca tcggtgccca cctgtaatgc cttgcgctcc agatcgcgca gaccatcttc
    18961 acggccttta cgcaggaagc ccacaaacgc ttctttatgc tcggtggcca gtttattttg
    19021 cgcaccacct gccggacgtt tcagctcaag cagctctttt tcaagatcgc ttttgaggtt
    19081 ttccagctcg ctgagtttcc cgttcagggt ttccacctgc ccggcaagct tgcctttttc
    19141 ctgctcaatc gcatccacgc gcttgtcgtt ctttgctttg aagtcgtcaa acttctgctg
    19201 cagctcctgc gcgacctgtt cgacatcttt aatatcaacc gccatcatat ttctcctgat
    19261 tagaagttca gatttttcag tgcattcagt gcagagccca catcctcagc gtcgcgcagg
    19321 gacagtgcgc catagccccc ggccatgaat gctttggcct gggtacggga gagtccgaca
    19381 tcacgcagga ctctttcgat ttttttctgt tcggggattt ccccgcgggc cagtgcgttc
    19441 ttgacgtcgc tgatccgcgc ctcgtcgtta gacgggaacg tcaccaggct gacttcccag
    19501 aggtcgattt ctttcagcag aaaggcttct ttgctccggt cgtattccca gtctttcagg
    19561 acgtacccaa tagaaaggcc ggttaacgaa ccggccttca tgtgtgcatg tgcgcgtttt
    19621 gcgaggggat catcatcaat aagcaaccgt cccctgacgt aaagcccgac atcgtcttcc
    19681 ttcatttcgg tgtaaacacc gatgggttca tccatgcggt gctgccagag cagcgcaggt
    19741 aacgcttttc tgtcactcca cgcccgcagg gaagcagcaa atgccccgga catcaccaca
    19801 tcatcgtggc tgtcctttac accaaagacg gagccatacc cttcaaactc accggagtca
    19861 ctgacagatt tcagactcag cggtacatca agacgttgtt tcgtctgcat tggcgttatc
    19921 cttctgctta ccggctttac tgccatcgga gggtttcgtg gtcatgttca tcggtgtgag
    19981 atagacatca ccaccgggac gcggattcat atcttccagg tcgcggcagt cattgggaga
    20041 gtaaattccc cagttgatcc cggtggcgta ggcttcaaaa cgggacttca tatccccgcg
    20101 cagtaacgcc ccggcgttaa atttggcgta ataaacgccc tgcttacttt ttcgtaccag
    20161 tccggtgttg atccgctgtt cgatgcgggt cagatacggc accagtgaat agttgataaa
    20221 tcccagcccc agctcttcga tattgttgaa ggtggcgcga tcggtgttct gcaccatgtg
    20281 caacggcacc cggaacagac gacagatttc ttcaagctga aacttgcggg tttccaggaa
    20341 ctggctgtcc tcggcgttca gcgccatcga cttccagtcc agccccatct caaggatcat
    20401 cgggcggtga gcattgccaa gcccggtgtg acgctcctca aaatctttct tcaggcgctc
    20461 ataagcctga tctgacagcg tctgctctgt acgcaacaca cccgacgtca ccgcgccatt
    20521 gctgaacagt ctggccccgt gctcttcggt cgctgccgcc agcgatattg cctcgcgggc
    20581 ataggcgatg ggattcagcc ccaccagtcc gtccagcgtc agcgtgcgca catgccagat
    20641 atcctcctgg ctcagtacat ccgtggagcc atccgggaat gtgacctgat agaccggctc
    20701 ccagctactg ttaagcttcg gtaccacaca gccgggatcg acgggcagca gttcagccac
    20761 ttcgccaaat gctttcactt tgtaggcgta aaagtttccc cgcaggcaca gacaggtgac
    20821 caccagctcc cagaactcct gcggcgtcat atagccattg ggatgcgtgg agatcagctt
    20881 atgcagacgt tcgccagcgg ctctctgctt cagactgccg ttcaggtgat acaggttgca
    20941 gggcaacatc ccgaccgact ccgccagcac cctgacacag gaaaaaaccg ccgtcagtcg
    21001 catggcccgc tggctgctga tctgctttcc ggtataggtg tcgtaggaca acccgatagc
    21061 atccgccagc tctgctggcg tggtcaccgg tgcgtcactt tttcgttgaa ataatcccga
    21121 aaagaacact atttacctcc gccgacagac gactgtgtac ggtcgagata tcgcgccacc
    21181 agccacgacc agaacagaca caacgccccg gcaacaacaa accccgccgg gggataaatc
    21241 agccaggcac catacgccag caaaagcgcc cccagcacgc ccaccagagg cgcgagaatc
    21301 agcatgatca taattacctc agttaaagcg agcggatccc ataggactca atgtggtcag
    21361 acagcgtgtc ttctttctcg tacagcatgg ctctgccaac cgccataatc agcgcaactg
    21421 caccatcgat tttgttttcc gcctgctctt tgacgggctt cactaaatca tcgttacctg
    21481 gcatgttttt gccgaccaca ttgccgatac accaggtcat gatgggattg ccgtcatgat
    21541 gaaagcgtcc cgattcaatc gctgcctcca gctctttcat cgggtcggac atattggcga
    21601 agttctggac gatagtaacg ggattcaggt cttcatcagc aaggtcatgt gacagcccgg
    21661 tcgctccgaa agggtcgatg ggtgactcac tgaccgggct gattttgttc gccgctttgg
    21721 cctcttcgag gatgtagcga taatccacct ctgcaccatc ggtaacggtc agaacgccca
    21781 tttccaccca tttctgaaag cgttcggctg tccggcgatc ttcatttttc tcgacgctat
    21841 acaccgtgtc atacggtacc cagaaacgcg gggccacact gtagtaatgc gttttaccgt
    21901 caatctcgcg ggtataaagt cgcgccatgc tgttcatatc cagcttacgc gccaggtcaa
    21961 aggccagaat gcacggctgc ccctcgaact gctcaagagt cagtgattta tcctcgcagc
    22021 tctgccagct caccaggttg aaatacgccg aacgcgccga cacccagata ttgaggtgtt
    22081 ttgttttaaa gacgtttgcc agacgggcgt tatttttcgc acgctgctgc tgacttaaca
    22141 aaaattcgcg ataaaccgac acgccaatat ttggattggc tttttccagc acctgcgggt
    22201 cggtccagtc gtcaccttca tcaacggtat agatgatccc gaacagttca tcgttaggca
    22261 ccgagccgtt gagcatctcg atgacttccc gccgtttgtc gtagcacggc ccctcaatgt
    22321 tgtacccggc ggtggtgatg gcccacatca gtggctgacg tcgcgccccc atcccggtaa
    22381 gcattgtggt ataaagcgca tcggtggcat gctcgtgata ttcatcaacc acggcacagt
    22441 ggggtgatga accatcacct gggttgccga tcagcggttc aaaccgcgcg ccatcctccg
    22501 gacggttcat gtttgaggcg ttaacctcaa tcccgaacgc ttccgtcagc atgggtgtgc
    22561 gtttacacat cagtcgcgcc gggcgaaaga cttcccacgc ctgtttctct gtcgtggcac
    22621 cggaatacac ttccgcgcca aactcgttat cacaggcaaa acaatacagg gcaacaccgg
    22681 cagagattgc tgatttgccg ttcttacggg ggatttcggt gtacacctcc cggaagcggc
    22741 gcaaccgggt gcctttattg acccagccaa acgcacagca gatcacaaat agctgccacg
    22801 gctccagcgt gatgggcatc cgtttgaatg cccactcccc cttggtgtgc ggcaacagct
    22861 gaataaattt cgcggcccgt tcagccaggt ccttgtcgaa gcggtaacga aacgacttac
    22921 ttttttccgc catcaggtca tcaagatggc gctggcaggc ctgaatcaca aactggcagg
    22981 caacaatctt tccgcgcacg acatcccggg catactgatt tgcagcattt acgttggggt
    23041 aagatttccg gctcatgatt cgataatttt cagaaacggg ttagtggctt tcttctgccc
    23101 cgccaggcca atcagacgct ggcggctgct ggggtcgagt ccgagcattg cccccgtact
    23161 gctcatctcg gactcctgtt cttttttggc ggtcagctcc ggatttttga ccatgccgcc
    23221 cattgcaccg gtgatggtgt tgccctgtct ggcaatattt ttcacggcac gtcgccagaa
    23281 ctcataggcc acgcaccacc gctcaagcac tgcgaggtca gtcacgcaca gcaggccctg
    23341 accgcagagt tctttggttg tcagttgcca catgatcgtg gcgagaggga gctcttcttc
    23401 agcgaaccac tccggtggct caacaccttt gatgggcgta aaaacgggtt catctttgtt
    23461 cagggctcgc ttgccggggt ttccggccag cgccttgcgc gccgttggct tggggcgacg
    23521 cccggaacgc cccgccgttc cagccatatg cggcactcct ggttaaattt catttttcgc
    23581 gggtataaaa aaacgatggg gcgggcagtc cggaagacgt caggtcacag ggatttgacc
    23641 cgcccctccc ctctggcagt gggaactggt tcttacttca gccattcacg ggccgtcttc
    23701 gccttatgac acggccaaca caggctctgt agattactgt cggcatcagt gccgccacgc
    23761 gctttaggga tgatgtggtc aacggttttc gcctcacgca ccacaccagc acgcagacat
    23821 aactgacaca ggcctttgtc acgcttcagg acacgcgcgc ggatacagtc ccacttcgaa
    23881 ccgtagccgc gctgatgacg ggactggcct ggcttgtatt gcttccagcc ttcgcttttg
    23941 tggctttcgc aatagcctga cgagtcagtg gtggtatggg agcagccgcg aacacggcag
    24001 gcttttgggg ttcgtggggg catatgtact ccaatgaaga aaccagcgaa atagctgcct
    24061 tcattcgtag taaacctttt tcatcaacgc agtaatggat tctttgaaga gtcgcaatca
    24121 atacgactca ctaatgggga ggcatgtcca acgcgttgga cgagttgcct acttgattta
    24181 ggtgacactt tagaaggaca gaatgccttc ctcactcaaa taacatcaat taaggaggtt
    24241 caacatgctt cgttccaaaa gtcagttgac tgtaattatt actacgtcac cttatgccga
    24301 agaccttttc cgcttcactt tgagcctgat tcacttctac ctgaccggct cgcctctatc
    24361 cttctgatcc ccgctttatc cagattgcac tgccctagcg ccgacaacag actgacattc
    24421 aaatctaggc tacccgcata agtcagcggt tcagaaatgg ctggttgcgg ggtttcagcg
    24481 gtcagactcg ccggtaacgg tatagccgga actggcacgt aaactgtccg cgaactttcg
    24541 caaccggtca gcagcggcag caggcacagg gcgtacagca caatcatcat ccgcaacagc
    24601 cactttgata tcagcctggg ttctctgtga ctccagtgcg atctgttgtt ttgcatgctg
    24661 gttagcctcc agaactgtat tgacgatttg tagtgattgc aggacgttat tggtaatggc
    24721 tgttgctgat tcagcatttc gtacagcctc atcagcacgt ttcttttcgt gctgatattt
    24781 gctgtagtag tggttagcag accagatgaa agaaccgatg acagtaaaga agaatgcaga
    24841 gatagccagc ttatagctca acttcattta ccaccccacc agcctcttta aaccgggaaa
    24901 tcaggtcacc gattttatgt tcatactgac cgtaacctgc accaggtaac gacgcccaga
    24961 tattgctgca acgatcgata gcctgacgaa tatcgccgcg atcaatcatc ggtaaagcgc
    25021 cacgctcttt aatctgctgc aatgccacag cgtcctggct tttcggagag aagtctttca
    25081 ggccaagctg cttgcgatag gcatcccacc aacgggaaag aagctggtaa cgtccggcgg
    25141 ctgttgattt gagttttggg tttagcgtga caagtttgcg agggtgatca gagtaatcag
    25201 taaacagttc gccgccaaca ataacatcat aaccgtggtt acgtgtcggt tgtcgcccgt
    25261 tatccgttcc ttctgaccat gccaccatat cgaggaaagc tatacgctgg gaatttagta
    25321 cctgcataaa ttactcctta gagccaccaa acttattacc gattactcgc attgcagccc
    25381 cacgaatagc atcaacaccg atcagcccca caccaccacc aatggcaaca gatagtgatt
    25441 taggccatcc gacatactca agagcggatg caaaagtcag cgtcagagcg ccacagagta
    25501 gaatttcgag tgtttttcgc ttccagccgc caccaccgcc aaaataggca atacgtaaac
    25561 cagccataac aatcgacata atcactgcgc ccagcggtgt gtctccacgc caccagctct
    25621 ggaacaactc cagccaagta tttgggttat gaggcatttg tagttatctc tcacctcgcc
    25681 aatacaggag gtgccaattg agggaatacc acgtaccgca catcagaagc ggaaacgtca
    25741 aagaagccga gtcaatggat aaatgtggga tagaccagga ccaacgaatc cccagcccaa
    25801 gaaacgacaa aacccgctcg acggcgggtt taagctgtgt ggcgaagtaa ccactcttaa
    25861 cacagtaatc tagaaaatgc ggaccgcgtt agtgattttt tcaacttttt gatttatctt
    25921 attgaacaca agaaaccact gcctataaat catatcaacc caaaccataa aaaccaaaaa
    25981 atcaaatgat tacattaact agataaggaa caaagttaat gaaagaaagc cgcagcaagg
    26041 tcattcaata taaacgtgca gtcatcccta attgcacagc aacgcttcaa cagatcatag
    26101 aatctataat ttctgaaaat ggcgcagcct ataaagttag cgcaagaaga gaacagatta
    26161 acccctccga tagcaatagt ggatttagag tggttaaccg cagcagcact ttcaagacag
    26221 tgttattcgg gcaacttatt ttgtttgaac aaggtaaaag ccaaacactt atgacaatcg
    26281 ctgatgatgt caattattat gacatcaatg ctataacatc gaagcaaatt aaattagccg
    26341 aagacgaaaa aataagtgaa gctgaaaaac aaaaaataac acgagaattt attgattcaa
    26401 tactttactt tgggattcgt gataatcatg tgatgatcgt ccaatcaccg gctctaagga
    26461 ctaaagacat agaaagccat cttaattggt taatccattc atttagcact atttttaatg
    26521 gtgacgactt ccttgtactt caagataaac caactgaaga caccataaaa aaaatgcatg
    26581 aaagtccagt caagaaaata aatttcggca gtatcccaat caaaaattct gattctgaag
    26641 atgtagttaa aataaataat ataagtaaca caaacgacct ccccacaaca ataaaagaac
    26701 aggtcgaaaa agttaagaaa ataaaattca tgccaactgg taaaggtggc gatattataa
    26761 aagctgcttt tggcgaagca tggtttaata atttacaact ggaagattct cttgatgaat
    26821 ccaatttaca agttaatcta gaaataacct atttccgcaa gacaaacaaa gatggtcaac
    26881 gtgtactcga tactttagca acttcactca gaaatataga cgatgaagat attagtataa
    26941 accttcaagg tggtggcata attaaaggca gtgatttaaa actatccggt aaagtaaacg
    27001 tccaatataa caatggactt attgatgaaa atgatttata tttacaaatg gctgacttca
    27061 aaggttcaaa ctggagaagt aaaagttaaa aactagaacc aacaggaggc ggaaatgagc
    27121 ttctttaaat ttattataag attcttatcc gccttcgctt ttgggtttgc aatattttac
    27181 tttgcatcta gaaaaattga cttaagtggc atatctactc catggtcatt aataacctta
    27241 ttagcattcc ctttcagtta ttgtatcgca gcctttttta aagtttctga agccgacgaa
    27301 aacacatcac tatcagataa tgaactaaga agattacgac cgataattga tgcaaaaaaa
    27361 agacatcttg gatttcttat ctttttttat cttattgctg ctttttcagg agcaattg
//
LOCUS       DAJTRB010000058        26608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTRB010000058 DAJTRB010000000
VERSION     DAJTRB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.058"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(11..151)
                     /locus_tag="OO330_002515"
     CDS             complement(11..151)
                     /locus_tag="OO330_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR5883340.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            393..686
                     /locus_tag="OO330_002516"
     CDS             393..686
                     /locus_tag="OO330_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5883341.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            758..1417
                     /locus_tag="OO330_002517"
     CDS             758..1417
                     /locus_tag="OO330_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5883342.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1509..1955
                     /locus_tag="OO330_002518"
     CDS             1509..1955
                     /locus_tag="OO330_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5883343.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2162..3062)
                     /gene="hlyE"
                     /locus_tag="OO330_002519"
                     /pseudo
     CDS             complement(2162..3062)
                     /gene="hlyE"
                     /locus_tag="OO330_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3435..3854
                     /locus_tag="OO330_002520"
     CDS             3435..3854
                     /locus_tag="OO330_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5883344.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3854..5122
                     /gene="umuC"
                     /locus_tag="OO330_002521"
     CDS             3854..5122
                     /gene="umuC"
                     /locus_tag="OO330_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5883345.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5168..5698)
                     /gene="dsbB"
                     /locus_tag="OO330_002522"
     CDS             complement(5168..5698)
                     /gene="dsbB"
                     /locus_tag="OO330_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5883346.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5844..7385)
                     /gene="nhaB"
                     /locus_tag="OO330_002523"
     CDS             complement(5844..7385)
                     /gene="nhaB"
                     /locus_tag="OO330_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5883347.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7606..8325
                     /gene="fadR"
                     /locus_tag="OO330_002524"
     CDS             7606..8325
                     /gene="fadR"
                     /locus_tag="OO330_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5883348.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8377..9909)
                     /locus_tag="OO330_002525"
     CDS             complement(8377..9909)
                     /locus_tag="OO330_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5883349.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10239..11537
                     /gene="dadA"
                     /locus_tag="OO330_002526"
     CDS             10239..11537
                     /gene="dadA"
                     /locus_tag="OO330_002526"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5883350.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11547..12617
                     /gene="dadX"
                     /locus_tag="OO330_002527"
     CDS             11547..12617
                     /gene="dadX"
                     /locus_tag="OO330_002527"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5883351.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12785..14199)
                     /locus_tag="OO330_002528"
                     /pseudo
     CDS             complement(12785..14199)
                     /locus_tag="OO330_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14810..16546)
                     /locus_tag="OO330_002529"
     CDS             complement(14810..16546)
                     /locus_tag="OO330_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5883352.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16641..17555)
                     /gene="ldcA"
                     /locus_tag="OO330_002530"
     CDS             complement(16641..17555)
                     /gene="ldcA"
                     /locus_tag="OO330_002530"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5883353.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17655..18266
                     /gene="emtA"
                     /locus_tag="OO330_002531"
     CDS             17655..18266
                     /gene="emtA"
                     /locus_tag="OO330_002531"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5883354.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18268..19002)
                     /locus_tag="OO330_002532"
     CDS             complement(18268..19002)
                     /locus_tag="OO330_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5883355.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKTAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHIIITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19203..19457
                     /locus_tag="OO330_002533"
     CDS             19203..19457
                     /locus_tag="OO330_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5883356.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19507..20967)
                     /locus_tag="OO330_002534"
     CDS             complement(19507..20967)
                     /locus_tag="OO330_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5883357.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20904..21461)
                     /locus_tag="OO330_002535"
     CDS             complement(20904..21461)
                     /locus_tag="OO330_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5883358.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLAQILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21487..22341)
                     /locus_tag="OO330_002536"
     CDS             complement(21487..22341)
                     /locus_tag="OO330_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5883359.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22341..23150)
                     /locus_tag="OO330_002537"
     CDS             complement(22341..23150)
                     /locus_tag="OO330_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5883360.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23160..23918)
                     /locus_tag="OO330_002538"
     CDS             complement(23160..23918)
                     /locus_tag="OO330_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5883361.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23915..24895)
                     /locus_tag="OO330_002539"
     CDS             complement(23915..24895)
                     /locus_tag="OO330_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5883362.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24895..25917)
                     /locus_tag="OO330_002540"
     CDS             complement(24895..25917)
                     /locus_tag="OO330_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5883363.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26266..>26608
                     /locus_tag="OO330_002541"
     CDS             26266..>26608
                     /locus_tag="OO330_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883364.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6687 a         6484 c         6865 g         6572 t
ORIGIN      
        1 agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcactatgg cctttgctga
       61 actcaacatt gacattctta cccgcagcaa agctcgtgga ggttagcagt gcaagcagca
      121 atacagcctt actgatgctc ttgattttca ttggtagtct cctgttcaga atgagtacga
      181 tgaactgtta taatataaca atcccttacg ggagaattcc agtggctttt ctgcgttcgg
      241 taaaatcccg gcgcggcatt ttttgccggg gggattactt ataaaggggc atggactcaa
      301 caaaccgcag aaatctacag ctggctggct tgtggcgcgg gtttcattga tgttagctta
      361 tgccaaaacc tggcatctta aagagtaaaa gtatgttttg tgtgatttat cgaagcagca
      421 agcgtgacca gacctattta tatgtcgaaa aaaaagacga tttttcgcgt gttcctgagg
      481 aactgatgaa aggttttggt cagcctcagt tagcgatgat tctgccgttg gatgggcgga
      541 agaaactggt caatgctgat attgaaaagg ttaaactggc attaaccgag caaggttact
      601 atttgcagtt accgccacca cccgaagatt tgctgaagca acatctttcc gtcatggggc
      661 agaaaacaga cgacactaac aaataaccga tatccggcgg tggcattatc tttgtcggcg
      721 cgggttttca tatccacgat aaggtgaggg gaacgttatg tatcaacatc acaactggca
      781 aggtgcgctg ctggattatc cggtgagtaa agtagtctgt gttggcagta actatgccaa
      841 acatattaaa gagatgggca gcgcagtgcc cgaagagcca gtgctgttta ttaaaccaga
      901 aacggcactg tgcgatctgc ggcagccatt ggcgatccca tccgatttcg gttcagttca
      961 tcatgaagtc gaactggcgg tgttgattgg cgcgacgctg cgtcaggcaa cggaagagca
     1021 tgtccgcaaa gccattgccg gttacggcgt ggcgcttgat ctgacattgc gtgatgttca
     1081 gggaaaaatg aagaaagccg ggcagccgtg ggaaaaggct aaagcgtttg ataactcttg
     1141 tccgctttcc gggtttatcc ccgcggcgga atttaccggc gatccacaaa atacaacgct
     1201 gggcctgagc gtaaacggcg aaaaacgcca gcaaggtacg actgcggaca tgatccataa
     1261 aatcgttccg ttgatcgctt atatgagcaa gttttttacc ctcaaggccg gtgacgttgt
     1321 gttgacaggc acgcctgatg gcgtcggccc gttgcaaagc ggtgatgagc tgacagtcac
     1381 tttcgatggg cattctttga caactcgcgt tttgtaatac tttttgccgc cagaaagcgg
     1441 cggcaaaact tgcatcgctg tgccagactg gttataaggt gcgttttaac gtaatggcgg
     1501 aacacctgat gagcgatgta cctttctggc aaagtaaaac cctggacgaa atgagcgatg
     1561 cggaatggga gtcgttgtgt gatggttgcg gtcagtgttg cttgcataaa ctgatggatg
     1621 aagacaccga cgaaatctac ttcactaacg tcgcctgtcg ccagctcaat attaaaacct
     1681 gtcaatgtcg gaactacgaa cgtcgtttcg agtttgaacc cgactgcatt aaattaaccc
     1741 gtgaaaatct gccaacattc gaatggctgc caatgacctg cgcttatcgg ttgctggcgg
     1801 aaggtaaaga tttacctgcg tggcatccgc tacttactgg ttcgaaagcg gcaatgcatg
     1861 gtgaacgtat ctctgtgcgt catatcgcag tgaaagaatc agaagtgatt gactggcagg
     1921 atcatatctt aaataaacct gactgggcgc agtgataaat ttaatagatg ctgatggggg
     1981 ctttcttaat atacgtctgg atgctcgcca tttcgtagcc ctgtttttga aagcgctctg
     2041 tcataatgag agttacccga taccttatta tcaacaggta actctcaatc tttaaaaaac
     2101 gtctgtgttt ttaaatgtga taaaccttat accttgagta catggagaga ataatcgctt
     2161 atcagacttc aggtacttca aagagtgtct ttttaccgtg tcttttctga tactcattac
     2221 aggtgttaat catttttttg gccgctgctt ttagcaaaga aagcattaaa tcatcataat
     2281 caacatagaa tctggttgtt tcagtttccg tttttatctc cccgatggcg gctatttcgg
     2341 tggttaattt caatttggcg gcatcgatat ctttattcgc ttgtttaacc gtgttagaca
     2401 gggtggtaaa gaaactctgc acagatttta acttgttctt caattctgga atcagtttcc
     2461 cttcaactac gcccgcagca atagaatagg aaatgattaa accaaatgga ccggcgacga
     2521 catcggctgc ggcaccggca tacgcttcct tcctgatttt atctacctgt gactggaaat
     2581 agctgctttt ttctgaaaaa tcattggtta actggctatc taacgccagc agtttcccgg
     2641 aagcgttgtt gaaactttgt gagcttacca gcagggattt ttgcgcttca ttcagcttcg
     2701 tgatgccgtc atccagtacc ttaatgaggg gcggatgctt tcttctcatt gtactcatca
     2761 aatagcaaaa tatacgctgc gagcaattgc gtcgcaacac cacaccattc atacactgtt
     2821 tgggttgctt caaaatactt atcctggcta tccataagta aggttttaat atcgccgact
     2881 aaaacggagg ctgcctgtga atactcctgt ttaaagcgac ttaactcttt tatggtttca
     2941 tcaaaggtct gccaggggat gacctgatcg agatatttat tataaagatc taatgctcca
     3001 tctgcggttt cgattgcgtt tttaactact tctaccgttt tatctgcaac gatttcagtc
     3061 ataatcattc gcctctttaa atatataaat tgtaatgaaa ctcctgtttt acaactatta
     3121 ataaatttta cttcatctaa ttcatagtta gccgggcggg atgcgtcaat gtctttattt
     3181 ctattaatat gataaatatc aaacaatgtt taatgtcatt atggcgaatg cttctattct
     3241 atttttttgc cgggtgatat ttttcatttc tgctggatga gcgtcgtcgc cagaaggcca
     3301 cgtgagcaca ggataagaga acgaaaaatc agcagtctat gcagcgacaa atattgatag
     3361 cctgaatcag tattgatctg ctggcaagaa cagaccactg tatataaaaa cagtataact
     3421 tcaggcagat tattatgttg tttatcaagc ctgcggatct ccgcgaaatt gtgacttttc
     3481 cgctatttag cgatcttgtt cagtgtggct ttccttcacc ggcagcagat tacgttgaac
     3541 agcgcatcga tctgaatcaa ctgttgatcc agcatcccag cgcgacttac ttcgtcaaag
     3601 caagtggtga ttctatgatt gatggtggaa ttagtgacgg tgatttactg attgtcgata
     3661 gcgctattac cgccagccat ggtgatattg tcatcgctgc tgttgacggc gagtttacgg
     3721 tgaaaaaatt gcaactacgc ccgacggtac agcttattcc catgaacagt gcgtactcgc
     3781 ccattaccat cagtagcgaa gatacgctgg atgtctttgg tgtggtgatc cacgtcgtta
     3841 aggcgatgcg ctgatgtttg ccctctgtga tgtaaacgcg ttttatgcca gctgtgagac
     3901 ggtgtttcgc cctgatttat ggggtaaacc ggtggttgtg ctatcgaata atgacggttg
     3961 cgttatcgcc cgaaacgctg aggcaaaggc gctgggcgtt aaaatgggcg atccctggtt
     4021 caaacaaaaa gatctgtttc gtcgctgtgg tgtggtttgc tttagcagca attatgagct
     4081 ttacgcagac atgagcaatc gggtgatgtc gacgctggaa gaactatcgc cccgcgtcga
     4141 gatttacagt attgatgaag cattctgcga tctgacaggt gtgcgtaatt gtcgcgatct
     4201 gactgatttt ggcagagaaa ttcgcgcaac ggtgctacaa cgtacccatc ttactgttgg
     4261 tgtggggatc gcccagacca aaacgttggc taagcttgcc aatcatgcgg caaaaaaatg
     4321 gcagcggcag acgggtgggg tggtggattt atcaaatctg gaacgccagc gtaaattaat
     4381 gtctgctctc cccgtggatg acgtctgggg gattggacgg cggatcagca aaaaactgga
     4441 cgcgatgggg atcaaaaccg ttctcgattt ggcggataca gatatccggt ttatccgtaa
     4501 acattttaat gtcatgctcg aaagaacggt gcgtgaactg cgcggcgaac cctgtttgca
     4561 actggaagag tttgcaccga cgaagcagga aattatctgt tcccgctcgt ttggtgaacg
     4621 catcacggat tatacgtcga tgcggcaggc catttgtagt tacgctgccc gggcggcgga
     4681 aaaacttcgc agtgagcatc aatattgtcg gtttatctcc acatttatta agacgtcacc
     4741 atttgcgctc aatgaacctt attacggcaa tagcgcgtcg gtaaaactgc tgacgcccac
     4801 tcaggacagc agggatatca ttaacgctgc tacgcgatct ctggatgcca tctggcaagc
     4861 gggccatcgc taccaaaaag cgggcgtgat gctgggggat ttcttcagtc agggagtcgc
     4921 gcagctcaat ttattcgatg acaacgcacc gcgccccggg agtgagcaat tgatggcggt
     4981 aatggatact ctgaatgcta aagagggcag aggaacactc tattttgccg ggcaggggat
     5041 ccaacaacaa tggcagatga agcgagctat gctttcacca cgttatacaa cgcgaagttc
     5101 tgatttactg cgggtcaaat aaatatagcg gcaggaaaaa agcgctcccg caggagcgcc
     5161 gaatggatta gcgaccgaac agatcacgtt ttttcgcttt aaacggctgg gaaatcacca
     5221 ccagcaccgc gacaatcagg taagcgataa aaataccgag cagccactgc ggcatttcca
     5281 tacctaaaaa atcccactga cgctcggcgc aatcgccaga ggcgacaaac acttgcggca
     5341 cccatttatc cagcggcagc cattccggga aacgaaccat aaaatcacag gtggcaaatg
     5401 gcgaaggata gagctgaagc atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac
     5461 tatacaacca gataaccatc gctacataac gcagcggagt tttcggggcg atcgcgccaa
     5521 tcagcgcagc acccagaacg ccgaataacg cgcagcgttc ataaatacag agcacgcaag
     5581 gtttcagtaa catcacatgc tggaaccaca gcgccgtcag ttccagtgcc agagcagtaa
     5641 acgccatcaa cagccacgcg ccccggcctt gtgaacattg gttcaaaaat cgcaacataa
     5701 tcatttccct gcaatatgca tagagtgcgc agtttaaacc aattcattcg ctgcgccacc
     5761 aggaggcagc aaaaatagga cgcaattgga caattatcgg gcgaaaaggc aaaccgggca
     5821 tttatatgcc cggtaagttg ttatcaaagc gttgctatcc agcccatttg cataaaccat
     5881 tcggttacag gggcaagcgt aaactcgacg cagagcaagc cgacgagtgt cagtacgagg
     5941 gtgtaaggca gagccatcca caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt
     6001 gcagaggtca gcaggaacag gaacgcagcc tgaccgttcg gcgtagcgac agagggcaga
     6061 ttggtaccgg tattaatggc gaccgccagc agctcgtatt gcttcaacgt gatagcgcca
     6121 ctttccattg ccgctttcgc ttcgttgata taaatcgtcc ccacgaagac gttatccgaa
     6181 atggatgaca gcagaccgtt gaaaatatag aacagcgaca gctgagcatg ttcagatgcc
     6241 tgcaacacaa actgaataat cggcgaaaac agttgttggt cgataatcac cgcgactacc
     6301 gagaaaaaca ccgtcaacag tgcggtgaat ggcagagatt cggtgaaggc tttaccgata
     6361 gcatgttcat cggtgacacc ggtcaatgat gttgccagaa taatgactga caaaccaatc
     6421 aagccaactt ccgccagatg caacgccagc gcagtcacca gccagacgcc aataatcgcc
     6481 tggacaatca gacggatttt atcctgacgg gtacgctggt ggcggctttg atcgtcaaac
     6541 tgttgcaaca cttcgcggac tttctccggc agcgtttcac cgtagccaaa ccaacgcagc
     6601 ttctctacca gcaggcaggt taacaggcca caaatcagaa ccggaacggt caccggcgac
     6661 atgcgcagga agaaatcgcc aaaatgccag ccagccgctt tagcgatgat caggttctgt
     6721 ggttcgccca ccatggtcat tacgccgcct aatgcggtgc cgacaccggc atgcatcatc
     6781 aggctacgca gaaagccacg gaactgttcc agaaccacgt tgtaatgctt gtcgatatga
     6841 ctatcgtctt gcaggtcggt gtcttcggta cgggaagagg ctacgcgatg ataaatacca
     6901 taaaaaccga ctgcaacgct gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg
     6961 gagaggaacg ctgccgccac gcaaaaagag agcgacagca gcattttgga gcgaatgctt
     7021 aacagcaaac gggtaaatat gaacagcaac agctgtttca taaaatagat acccgccacc
     7081 ataaacatca ataacagcaa gacttcaaga tttgccgcca cctcttcacg gacgtgttcc
     7141 gcgctggtca tgccgatgaa taccgcttcg atagccaaca gaccaccggg gagtagcggg
     7201 tagcatttca gggccatcgc cagagtgaaa ataaattccg ctaccagcaa ccagcccgcg
     7261 acgaaagggc tgatgaggaa aattaacggg tttacgatta agaaaatgat gagggcgagt
     7321 ttgtaccagt cgggggactg gcccaaaaaa ttgcgccata gcgcgcggcc ccaggagatc
     7381 tccatgatgg tttcccttac cttacaaata atcaatgatg tttttatgtt taaacgcaaa
     7441 gcttaacggt caggcaggag tgaggcaagt cttgatagtc aaggggaaag agatgcggaa
     7501 aatgaagcct tgatcccttt ttcttctttt tgtctgctat cagcgtagtt agccctctgg
     7561 tatgatgagt ccaactttgt tttgctgtgt tatggaaatc tcactatggt cattaaggcg
     7621 caaagcccgg cgggtttcgc ggaagagtac attattgaaa gtatctggaa taaccgcttc
     7681 cctcccggga ctattttgcc cgcagaacgt gaactttcag aattaattgg cgtaacgcgt
     7741 actacgttac gtgaagtgtt acagcgtctg gcacgagatg gctggttgac cattcaacat
     7801 ggcaagccga cgaaggtgaa taatttctgg gaaacttccg gtttaaatat ccttgaaaca
     7861 ctggcgcgac tggatcacga aagtgtgccg cagcttattg ataatttgct gtcggtgcgt
     7921 accaatattt ccactatttt tattcgcacc gcgtttcgtc agcatcccga taaagcgcag
     7981 gaagtgctgg ctaccgctaa tgaagtggcc gatcacgccg atgcctttgc cgagctggat
     8041 tacaacatat tccgcggcct ggcgtttgct tccggcaacc cgatttacgg tctgatcctt
     8101 aacgggatga aagggctgta tacgcgcatt ggtcgtcact atttcgccaa tccggaagcg
     8161 cgcagtctgg cgctgggctt ctaccacaaa ctgtcggcgt tgtgcagtga aggcgcgcac
     8221 gatcaggtgt acgaaacagt gcgtcgctat gggcatgaga gtggcgagat ttggcaccgg
     8281 atgcagaaaa atctgccggg tgatttagcc attcaggggc gataatccct tccgtttaaa
     8341 gagcaacccc ctcaaacgag gggttttttg ttgtttttac agatttccca ttcttggcgg
     8401 gcaacgttcc agcaactcga tgctgccgtc ttcgttttgc tgttcgagca tcacatcaaa
     8461 tccccacagg cgatgcacat gcttcaggac ttctttgcgc ccccgatcca gcggtgcgcg
     8521 attatgtgga atataacgca gcgtcagcga acggtcgccg cgcaaatcca cgttccagat
     8581 ctgaatattc ggctccagat tacttaagtt atattgcgac gataaccggt tacggatctc
     8641 ccgataacct tcttcattat gaatagcgga aatctccaga taattatgcc gatcatcatc
     8701 cagcacggtg aagaagcgga aatcacgcat cactttcggt gacagaaact ggctgataaa
     8761 actctcatct ttgaaatcac gcatcgcgaa atgcagcgtt tccagccagt cggaaccggc
     8821 gatatccggg aaccagtatt tgtcttcttc cgttggcgac tgacaaatcc gtttaatatc
     8881 ctggaacatg gcgaacccga gggcatacgg gttgatgccg ctgtaccacg ggctgttata
     8941 ggggggctgg aagaccacat tggtgtggct gtgcaaaaac tccagcataa aacgttccgt
     9001 tactttccct tcatcataca gatggttaag gatggtatag tgccagaagg tcgcacagcc
     9061 ttcgttcatc acctgagtct gtttttgcgg ataaaaatac tggctcacct tacgcacaat
     9121 acgcaggatt tcacgctgcc atgattccag cagtggcgca tttttctcca taaaatagag
     9181 caggttttct tgtggttcgg acggatagcg gcgcgcttca gcaaccgttt tctcttcctc
     9241 gcgcttcggc agggtacgcc agagcatatt gacctgactt tgtagatact cttcgcgact
     9301 tttctgccgg gctttctctt cttgcagcga gattttttgc gggcgtttgt accggtccac
     9361 gccgtagttc atcagcgcgt ggcacgagtc cagaagccgt tctacttcat caacgccata
     9421 acgttcttcg cactcggtaa tatatttacg ggcaaaaatc agataatcga caatcgaact
     9481 ggcgtcggtc caactgcgga ataagcaatt gtttttaaag aaagagttat gtccatagca
     9541 ggcgtgtgcc atcaccagcg cctgcatagt aatggtgttc tcttccatca agtaagcgat
     9601 acacgggtta gagttaatga cgatttcata ggccagccct tgctgaccgt gcttatacag
     9661 ccgttcagtc tcgataaact ttttaccgaa tgaccagtgc ggatagttaa ttggcatgcc
     9721 gacgctggaa taggcatcca tcatctgttc tgaggttatc acttcaatct ggtgcgggta
     9781 ggtatccagc cggtagagtt tcgccacccg gtctatctct gccagataaa catccagcag
     9841 gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg tccttattca tagaatcgat
     9901 cgtcgccata cgcgcacctc attgttgtcg gcgctctctg tgtggagcac ctcatttcaa
     9961 gcatagaaca cctgttaaaa accgcgtcgc cggagaattt ttttctttgc gatttcttat
    10021 tatcagagtg ccactaatcc gcttctgaac ggaattttat gctggataaa aagggcgttc
    10081 agcaggagat actaaagacg ccatattgcc gcagagtcag ggagatgtga gtcagctcac
    10141 cataaaaaag ccgtatgttg aataatattt tcaactgagt tatcaagatg taattagatt
    10201 attattcttt tactgtatct accgttatcg gagtggctat gcgagttgtc atactgggaa
    10261 gtggtgtggt aggcgttgcc agcgcctggt acttaaatca ggcaggacat gaggtcaccg
    10321 tcattgatcg ggagccgggg gccgccctgg aaaccagtgc cgcgaatgcc gggcaaatct
    10381 cccccggata tgctgcaccg tgggcagcac caggtgtgcc tttaaaagcg attaaatgga
    10441 tgttccagcg ccatgcgccg ctggcggttc gtctcgacgg tacgcagttt cagttgaaat
    10501 ggatgtggca aatgttacgt aactgcgaca ccagccacta catggaaaac aaagggcgga
    10561 tggtgcgtct ggcggaatac agccgtgatt gcctgaaagc attgcgcgcc gaaaccaata
    10621 ttcagtatga agggcgtcag ggtgggacgc tgcaactgtt ccgtaccgaa caacagtatg
    10681 aaaatgcgac ccgcgatatc gccgtgctgg aagatgccgg cgtaccgtat cagctgctgg
    10741 aatccagccg cctggcggaa gtggatcccg cgctggcaga agtagcgcac aaactgacgg
    10801 gcggcctgca gttacccaat gatgaaaccg gagactgcca gctgtttacc cagaatctgg
    10861 cgcggatggc agagcaggcg ggggttaaat tccgctttaa tacgcccgtt gaccaactgc
    10921 tttgcgacgg cgagcaaatc tacggcgtga agtttggcga tgaagtgatt aaggccgatg
    10981 cgtatgtgat ggcgtttggt tcttactcga cggcgatgct caaaggcatt gttgatattc
    11041 cggtgtatcc gctgaaaggc tactcgctga ctattccaat tgcgcaggaa gatggtgcgc
    11101 cagtatccac cattcttgat gaaacctaca aaatcgccat tacccgtttc gataaccgca
    11161 ttcgtgttgg cggaatggcg gaaattgttg gttttaacac cgagctgttg caaccgcgtc
    11221 gtgaaacgct ggaaatggtg gttcgcgatc tctatccacg cggcggtcat gtcgagcagg
    11281 cgactttctg gacgggtttg cgcccgatga cgccagacgg tacgccggtt gtcgggagta
    11341 cacgctttaa aaatctgtgg ctgaataccg gtcacggcac gctcggctgg acgatggctt
    11401 gcggttccgg tcagttgtta agcgatctgc tctctggtcg cacgccagcg atcccatatg
    11461 aggatctaag cgtagcgcgc tacagccgtg gatttacgcc atcacgtccg ggccatttac
    11521 atggcgcaca cagctaagga aacgagatga cccgtccgat acaggccagc ctcgatctgc
    11581 aggcattaaa acagaatctg tccattgtcc gccaggccgc gccgcacgcg cgcgtctggt
    11641 cggtggtaaa agcgaacgct tacgggcatg gtattgagcg tatctggagc gcgctcgggg
    11701 ccaccgatgg ctttgcatta cttaacctgg aagaggcaat aacgttacgt gagcgcggct
    11761 ggaaggggcc gatcctgatg ctggaaggat ttttccatgc tcaggatctg gagatttatg
    11821 accagcaccg cctgaccacc tgcgtacaca gcaactggca gctcaaagca ctgcaaaatg
    11881 cgcggctaaa agcaccgttg gatatttatc ttaaagtgaa cagtgggatg aatcggttgg
    11941 gcttccagtc cgatcgcgtg cttaccgtct ggcagcagtt gcgggcaatg gcgaatgttg
    12001 gcgaaatgac cctgatgtcg cattttgctg aagcggaaca tcctgatgga atttccggcg
    12061 cgatggcgcg tattgagcag gcggcggagg ggctggagtg tcggcgttcg ttgtccaatt
    12121 cggcggcgac tctgtggcac ccggaagcgc attttgactg ggttcggcct ggcattattt
    12181 tgtatggcgc ttcgccgtcc ggtcagtggc gtgatatcgc caataccgga ttacgtccgg
    12241 tgatgacgct aagcagtgag attattggtg tccagacgct aaaagcgggc gagcgtgtgg
    12301 gctacggcgg tcgctatact gcgcgcgatg aacagcgaat cggcattgtc gccgcaggat
    12361 acgccgacgg ttatccgcgc catgcgccta ccggtactcc tgttttagtg gacggcgtgc
    12421 gcaccatgac ggtggggacc gtctcgatgg atatgctggc ggttgattta acgccttgcc
    12481 cgcaggcggg gattggtacg ccggttgagc tgtggggcaa ggagatcaaa attgatgatg
    12541 tcgccgccgc tgccggaacg gtgggctatg agttgatgtg cgcgctggcg ttacgcgtcc
    12601 cggttgtgac agtgtaactt gttgtaagcc ggatcggagg cagcgtcttc tggttgcgaa
    12661 aaaatcatcc atccggctgg tcagcaactg tagttgttaa tgtgacagag ccattgccca
    12721 tgatagtgtc cattaaaaga atggacacta tttccccgga acctgaactc gccgcacagg
    12781 cgttctacat aaaacgctta cgcttcattg ttgactcgga gtcgacttca gataaatcac
    12841 gcttcaccct tgatgcagat tcgggttcat gttcagattg ttgattaaca ggctgcgagt
    12901 cctgttgtct ttccagactc aacaggccca actgttgatc caggagctcc aggtcctcct
    12961 gtaccttcac gcgctccagg agttccgggt catccaggaa ctccagatcc ataaggtcct
    13021 ccagctcctc gagtccttcc aggtcaacct agccttcctt gtgcaaaaag tccaaaaggt
    13081 ccttcaggcc ctgctgtttt ttcaggccct cgacggcttt gagcagcgtc ggtaaattgt
    13141 ctttgtccca cgggttacca ttacagtcaa acaccttcag gctctggggc tgcggcacct
    13201 gtaccgtttt aaacttgttt tctccacagt agtaaattat tagtttgggc ggcagccgtg
    13261 gtgaagagat caaatcgtta ctagaacagt caaatacttt caggcgagtc aagttctcca
    13321 gcatctcatc aggtattgag ttaatattgt tgccggcaca ggatagttta tacaggtact
    13381 ggggcatcga cggcggtaaa aattgcaaga agttttcaga acagttaatc actctcaggt
    13441 tagtcgacag ctcaggcagc cagataatac tgttttcata gcacttcagt accatcagtt
    13501 ttgagggctg caccttcggt aacaagatca agacgttatc tgtacagtca agcaccagca
    13561 gccactttgg catcaccttc ggtaacgcgg tcaacacgtt tttactgcag taaaattcaa
    13621 tcagatgtgg aggcaaattc tcagggagtg aagtcaatcc caggttgcta agattgagca
    13681 tactccgtct tgtaatgaga cattccttca ttctgcgcaa tgcttctgcc cggttttcaa
    13741 tccggcttgc tggcaggttt tcttccagga tattttcctt gatttcttcc agactttctt
    13801 tcaggtcttc ttggggaatt tctgactgtt tgttttgctg gttttcttcc aggatttcgt
    13861 acaggttttt ttgcaggttt tcttgcaaac catcctgctg tatagcccat tcgcaccatt
    13921 tggcgaccat catgaactcg ttatgttgct gattcagacg agtccccaac gtgatgatgg
    13981 tattgttgtc cacctgtatg ttcactttgc ccgcgatact gaggtcaatg atctgccagg
    14041 gggcatcacc ccagctgaat ttttgcgggc tgagatactt catgttctgc aaaatttgat
    14101 tcttgagaag agccgccctg gcgggcgtcg tacgcctggg ttaacggaag atgggggaat
    14161 ggcgggggcg aatgggcaga tgtctttaaa gctagccata atgctacgtt tccttgaaat
    14221 aaatatcctc tggcagcgcc ggagacgtga atgtggtcac cgcaaaggag ccacaggacg
    14281 tcatttgcgc tgatatcact actcatcgtt gactttttcc ctggtcagaa atgccgtgtc
    14341 gggtctggta catgctgtat tacggcctag gtagctctga cgtattaaag aaaatacgag
    14401 agtgaacgac ccggaaataa tcgttattaa gcaaaggaat aactataaga aatcgctcat
    14461 gcatccggtg ctacaggcgg atgaaacgcc tattgtgtgg gctatgtcag tgcggcaggt
    14521 tgcgtggggg aatatgttca ggcgatgtca ggcaggggcc cctgatggtt gaccggtaat
    14581 gcggggctgg tttttattac ctgttacagt cggaaaaagc ccttacgctg actttcttga
    14641 gccggtgttt tgagttcatt agccagagcc tgcaatggtt tgtcatccat aatttaacct
    14701 tcttctgatg ttgggatgag catatcaaaa tcaggaaatg acaagtttat tacaggctca
    14761 ggctgcgttc agtaaagcat cgcatccggc gatagatgct gcattaaatt taagattcag
    14821 cttcctcttc agccacccga acgccaatct tcaacacttc attatcttct ttctcggcca
    14881 ccgtccagat catcccggca aactctacct ggtcaccgac aaccggtgcc gcgcctagca
    14941 actgctggac aatttcaccc agcgtttgct gtttatcacg atattctcgc ccgtcttcca
    15001 ggccatatat cagcgcaaca tcagcatatt tggcgctggc ttcgagaatg aagtcaccaa
    15061 agaagcgttg atccagcgcg accggtggcg actggctgaa cagtttaccg agcgccggga
    15121 gatcgcgttc ccgaccaatt acacacaata catcgccttc gcgcagtcgg gtgctgccgg
    15181 taggatgaag caactggtta tcacgaaaca gggctgcaat acgcgtctct tttggcatat
    15241 gcaaatcgcg cagtgccgca cctacacacc atttatcagc actcaattga taaacaaact
    15301 gctcccacgg attttccgga tgaatatcca ggccaacgcg tgacaccgga cgtccgacgg
    15361 gcggaaccac cactttggct tttttcgccg cccacgagag tgatgttccc tgcaagagca
    15421 gtgaaaccag aaccacaaag aaggcgacat taaagaacag tcgcgcattc tccagccccg
    15481 ccatcatcgg gaacacggct aggatgattg gcaccgcgcc gcgtaatccc acccagctga
    15541 taaacacgcg ctcacgcaga ttgaaaccgc ggaaagggag caatccagca aataccgaaa
    15601 ggggacgggc gaagaatatc atccatgccg acaaaatgag cgccggaatg gcaataggca
    15661 gcagatcgct tgggttaacc aatagcccca gcaccaggaa catggcgatt tgcgccagcc
    15721 aggcgaggcc gtcgaaattt tgcaggatgc cgtagcggtt gcgaatcggc cgattaccca
    15781 gcagaaaacc gcacagataa acagccagaa taccgctgcc ttccagcgcc gtggttaaag
    15841 caaaaattaa aataccgccg cttaatgcca gcaatggata taatccggca ggcagggcga
    15901 tgcgattaat catttgcagc agtaaataac caccgccaag accgatgaca atcccgaggc
    15961 caaattgctg cagaatatcg accacgaaca tccaactgac actgctttca tgctgctgga
    16021 tcattgcaat cagggtaatc gtcagaaaga ccgccattgg atcattactg ccggattcaa
    16081 tttccagcgt cgagccaaca cgttcgttaa gccccttacc acccagcaga gaaaagaccg
    16141 ctgcagcatc ggtggagccg acgatagcgc cgattaataa gccttcaatc aaatcaagat
    16201 taaacagcca cgccgccatc atgccggtta aaccagaggt gataagtacg cccagcgtcg
    16261 ccagcgacag cgccggtcct aacgccacac gaaaggaact ggcctgagtg cgcatcccgc
    16321 cgtcgagcaa aataatcgcc agcgccaggt tacttaccat gtaggcgaag gggtaattat
    16381 caaacgggat accgccgacg ccatcgactc ccgccagcat gccgatcgcc agaaaaataa
    16441 ccagaatagg aatgccaaga cgggaagaaa atgaactaag taatatactg ctggtgacaa
    16501 ggatggatcc taagatgaaa aggctaatta ttgttgtggc atccaacggt cggttactcc
    16561 tgattacgct gtctcttata taaaccctac catattagcg gcagagacag cgttttactt
    16621 agccctgaag cgtgattttt ttacatttta agaacaggat gaccgctaat cgtcaactga
    16681 gcgccttccc gggtgttatt cagaatggca tgtgcgccca acggcagcgt aaccgtgcgt
    16741 tgttcatgac caaaatcgag gccggtaatg agtggaatcg acaggcggga acgcaaaaac
    16801 gcgtacactg actccaggtt gtaacctgcg tcataatcat tgggcgtgct gccgctaaag
    16861 ctaccgagaa taatcgcctt ctgacgtggc aaaattcccg catgatagag ctgcaacagc
    16921 atacgttcga cgcggaaagg gtgcacgtta atatcttcca gcaccagaat accgttctca
    16981 atttttggca tccacggtgt accaatcagt gaaatcagca tcgcaagatt gcctccccac
    17041 aacgtgcctt cggtctgaca tgtcgggcct tccccttgcc attcaatggt gaaggtttca
    17101 ttgcgtaacg ccagccagaa atggtgctcg gtaaaggcgt tcagttcatc cgcaccaaag
    17161 tttgctacca gcatggggcc gctaaaggtg atgacattgc cttgcgccag aagaccgcac
    17221 tgaatggcgg taaaatcgct atgtccgcaa atgagtaacg ggtcatgttg ttggcgagcc
    17281 accagtgcct gccagtcaat atctgccagt aaacgactgg caccgtaacc gccgcgtaca
    17341 gccagcacga tggtgttggg ggtagtcagt ctggcaaggg aattgagatc ttccagacgc
    17401 tccgtttccg tacccgcaaa acgctcacaa cgacgggcaa taacctcgac gttattgact
    17461 tgatgccccg cgtcggtaag gcgctggata ccacgcagcg cggcgtgctg tttaatgctg
    17521 taacccgatg gggcaattaa gtgaaacaga gacatggcaa ttccttgctg acaacagaaa
    17581 cgaaatgtat atcatgccgc ttaggtgtgc cgttgtcacc tcaacggcga ttccaggcta
    17641 taaggataga agaagtgaaa ttgagatggt ttgccttttt gattgtgtta ttagcgggtt
    17701 gttcatcaaa gcatgactat acgaacccgc cgtggaacgc gaaagttccg gtgcaacgtg
    17761 cgatgcagtg gatgccaata agccagaaag ccggtgcagc ctggggcgtc gatccacaat
    17821 tgatcacggc gattatcgct atcgaatcgg gtggtaatcc gaacgcggtg agtaaatcga
    17881 atgccattgg tttgatgcag ttaaaagctt caacctccgg acgtgatgtt tatcgccgta
    17941 tgggctggag tggtgagccg acgaccagcg agctgaaaaa tccagagcgt aatatttcaa
    18001 tgggggcggc ttacctgaat attctggaaa ccggcccgct ggcaggcatt gaagatccga
    18061 aggtactgca atatgcgctg gtggtgtcat acgctaacgg ggcaggtgcg ctgctacgga
    18121 ctttctcgtc agatcggaaa aaggcgatca gcaaaatcaa cgatttggat gctgacgagt
    18181 tcctcgatca cgtagcgcga aatcatcctg cgccgcaggc tccgcgctat atctacaaac
    18241 ttgagcaggc actggacgcg atgtaaatca gtcgcgcact ttgtccgctt tttcccgggc
    18301 ttctcgctcg agagagaaaa taatccgctg taattgccgc tccaccgtcg ggctgacgtt
    18361 aagaaaacgg aagctcagac ggggagtggt gatggtttca ttcttgccat caatcacttt
    18421 gcgctcgctg gtggagatta actgggcgtc aaagtgaaaa acaccccatt gccccatgtt
    18481 gacttcaatt tgagcgaagc gcataccttc ttgtaattcg gcaggctttg ctgtttccag
    18541 taatgcgccc atgccgccta acgacaaatc atacaagcgg aaacgtaacg tactgttatc
    18601 cgccagtttg gtctggcaaa aataaggcgg atggagtggg gcggagatgc ggaaatatcg
    18661 gcgtcgttgt acaaaccata aggtgggagg cggtacggta ataaatgccg gaagctgcaa
    18721 gtattcactc tgctgtagtt gttcaacagt aaactcgact ttcgcaccct gagtttcggc
    18781 ggtaatgata atgtgctgtg cctttagcac ggcgatgttg tcttcggctt gactgccgaa
    18841 atccagcacc agtttatccg gggttattgc cagtattttg ctgatcagct gcccgccatt
    18901 ccaactgaga cgcaaaggaa ttgcggtttt gtgcaaatcg cgtaacacgc ccaggacggc
    18961 taacggattt tgtttcagga actgctcatg gtaatgactc acgcggaaaa actcctgact
    19021 gacaaactgt ctttggttta tcggtcatag ttaacaaaac ttaatacaaa tgcgtgaata
    19081 ttttttacat gttgttctta aatcagccga caaacgccca gcatcgatcc cgcccttgcc
    19141 tatacttaga gcgttgacgt aagcatttct tgcgaccgat tcatcgaaac gagggcctga
    19201 acatgggaat tattgcctgg attatttttg gcctgatagc cggcattatc ggcaagctaa
    19261 tcatgccggg gcgtgatggt ggtggatttt tcctgacctg tattctcggg atagtcggtg
    19321 cggtggtcgg cggctggctg gcgaccatgt ttggcattga cggctccatc agcggtttta
    19381 atttgcacag cttcctggtg gcggtggtgg gcgctatcct ggtgctgggc gtattccgcc
    19441 tcctgcgaag agaataagat tttcataagg cggatagcga tacagatgcc gctatccgct
    19501 ttcacatcag aacgtatact cgacacctgc ccagaaattg cgaccttctt caataaaccc
    19561 ttcactgtag gcatattggg tatcaaacag gttattgacc gacgcattga cgctgaagcc
    19621 atgacctaag gtgtaatcgg ctcgaatgtg ggtcaccgca aaaccggcgg ctttttgtga
    19681 accgtcactg ttgctgtagc tggaggaacg cgcctcttcc gacagcgtta cgcttaacgg
    19741 ctcccatggt ttgagtgtca tccatgcggt cattgtctgc gttggaagat cggttatctt
    19801 gccgatgtct ttgcgtttgg cgtcagcgtg gatcagagcg tagctcaatc ctacatccag
    19861 tatattgctg attttcccct taataccggc atccagaccg ctgtaatcca ccgtgccgct
    19921 gttctgattt tgaatggtat cggcattgat attgtgcgag aggatggcat cactcacccg
    19981 gttatagtaa acgctgacct caaatcccca gtcgcgcgtg aaggcaccat tccaggttaa
    20041 atccaccccg cgagcgcgtt ccggcttgag ctgcgggtta acaatcgcta tctggttata
    20101 cgcaggtttg gatgtggtat agcgttcttt cagcgtcgga aagcgtgtgc ggtcatagta
    20161 cgaaagcgcc agagtgtctt cattggcaaa gtggtatttc cccatcacct gccagttaaa
    20221 agctgactga ttgttgtcgt cataatgggt gatgctgcca tctttctcgt gttttttcgc
    20281 ttctacgcta tcgcgccagt catagctgat tcccgccacg acatcgacat tatcagcagc
    20341 agcccattga tattcactgg cgagcgacca ggtacgatct tcatagcgat cgtaagcggc
    20401 gtgcggcgca cctttttccc ggtgtacgtc atctttccag ttaacggcaa acgacagcag
    20461 atcgttttca cgcacatcgg ctgccagttg taatccggca ccgtcgctgt aatcggaata
    20521 atggctgtag ctgccttttt tatttttcaa atcagccagc gagttgtaca tcatcagcgt
    20581 attttcaaag gtgtcgcgat acagccgact tttcagggta aaacggtcgt ttagttgggt
    20641 cgctccctga taataaaaac tttctttgtc atactctggc cactgccagt agcgtgattt
    20701 ttgaccacaa tttccgctgt atggcgggtt atctttttca ccatcctgct taatgtaagt
    20761 cagcgtgtat tcatcgtttt cgcgcggtgt aaaaccgagc ttcacaatgc cgcgtttatc
    20821 atcagccgat gaattaatca tcttgccgtg tttgcctgca atatcattat ttacaccatg
    20881 cggcaggccg agaaaatcct gctttagctg gctaccgctg acttgcaaat acccaagatc
    20941 gctgctggcg gcaaatgaag catgcatatc gtaggcattg tcctgactac ggctccatcc
    21001 ctggcgatat cccagacttg cttcttcagg gtggtgatat taatggctcc gcccatctga
    21061 ttaggcccat gaagcagcga cgaataccct ttggaaactt cgactgcccc cagattgttg
    21121 gtcagaatct gcgccagatc gaggttgccg tcatagggga cataaatggg cacaccgtcg
    21181 aaatagaccg gcacctgccg actatcaaag ccacgaactt ttacctgttc ttcgttgcgg
    21241 ctgcctgact tttgcagcac cacgccgggg acgacactta atgcctgagc gacattctgt
    21301 ttatccaagg ccttcatggt gggttgatcg agaacggtgg tcgtcgtcga tgataccgga
    21361 ctggaccata cggtcagcgt gtcgctctcc tgagccgcta cggcctgctg ggtaaaggca
    21421 agtgataatg ctgtgcagag ataacgtttt ttgagtctca tagaataaat tccgtttaac
    21481 aaagaattaa atagaggctg tgggttgcag actgacttta atgtcagctg gtgccgcgta
    21541 ataaggcgcg gaggtaataa aaaggcgaat gccgcagtcg aggtaatttt tgagtgtcgt
    21601 cagattaatt ccgccggtca gagccagcgt gcagtgagga gccagcgacg gcgctatttg
    21661 cgcaatttca gttgcctgtt gcggactaaa tttgtcgagc tgaagtacgt ctggttgtgc
    21721 gcgtaacgcg gcgatagcct ctttcggcgt gtcggcttca acaacaattt tcttctcggg
    21781 tgcgtggcga cgtaactgag cgattgcgcc tgaccagccc tgattgtcat gaagaaaatg
    21841 gcggtggttg gcaaacaata atatggtttc cgcacatccg gcacgatgaa ttagtcctcc
    21901 ggcagccaga attgcctgcg aagccagtaa gcgggtgccc ggaattgctt ttcgggtgca
    21961 ggcgatattg ccgtcaggat aacgttcacg aagcaatgcc agcatttgag cgagataatc
    22021 agaaacaccg caactccact ccagcacatt ctggactgcc ttccatccct gatgaagtgc
    22081 tgcggcatta ccctgcgcac ggattagccg ttgacccgcg ttcgcttgtg aaccgtcgct
    22141 gaccgcgtca tcaatagtta atcccagcgt agttaacatc ttacacgcga cagaaacgcc
    22201 gctgacgcag ccgccctgac ggtgaaaaaa cgctatatag ccaggctggt gtccaatatt
    22261 taatgctcgc gtggtcaggt cacctccctg gatatcttcc agcagtaacg catcgatttg
    22321 tgcctgagaa aggaaaatca tcacagtgcc tcctgcggta cgctatccca ccagatgagc
    22381 gcccagtcgc ggctggcagg ggcgagggca cgagcatctt gttgttgata ccagtgatat
    22441 aaccgctcac gctcatcgtc gttaagcgcg ccaagactcc agctgacgtt ctgctcaaaa
    22501 cgttcccagg tgctgttatc ctgttggcaa ttttgtccac ggataaaatc aacgtgggca
    22561 taaattccca tctgatacag gacgttgagc gcaaagatat agttgggtaa ttcgatcact
    22621 tcccgaccag ccgcccgctg aatggctgga gagacgaacg aggtgctgac cagatgagtt
    22681 gtatagacgc gaagccgtgc ctgattattc agtttgctca ttgcatggcg catatcagcc
    22741 accagagtgg agcgggaagc cacggcaata tcgcagcgtg gcaggtcact ccagtcctct
    22801 tcccaggcgc gctggatcca gtggacattg tcagccttca gtgcggcggc gcggcgggcg
    22861 gcaacgttca gcatcccctg actgtaatca acgccataaa ccgtagtgag tttgtcagcc
    22921 agcgccagcg atacggtgcc tggtccgcag cccatatcga ataaggtctg agcgccttgc
    22981 aggtcaattt tagcgatcag ttgttgcagg tagctgtccg ttggcgaggc gcaattttcc
    23041 gccattttct ccgcccgttg atcccagtga tcgggcgttt tttctgtgcg gtgagccagt
    23101 ttgagttgct gcagataaag gtctgcaaaa tcaatatcat caatgagcat gattgttcct
    23161 tagcggctaa ttgcagagag gtgatgatga atttggtcgg cagaaacccg atacagggcc
    23221 gccagtttgt tcgttgttaa ctgttcggtc ggtaagcctt gtgttactcg tccatctggc
    23281 tctacttgga taatgctgtc ggcaatggcg ttggcatgga gcggatggtg agtcgacatt
    23341 aacattgtca tgccgttatt tttgagctgc gccagcgtgt ccagaagttg gatctgatga
    23401 ccgaaatcga ggctggaggc gggctcatcc agtaacaaca agcgcggttg ttggacgagt
    23461 gcacgggcga ttaaagccag ctgacgttca ccgccgctaa gcgtattcca gcgtcgtgtc
    23521 gccagatgca aaatgcccag cttttccagt tgctccgtgg ctttcaggcg ttcttgctta
    23581 ccgggaacgg aaaaagcgcc tatggtgggg gccaggccca tcaacaccat atccagcaca
    23641 ctgaaagcaa aaataccgtc gtgcgcctgc ggcacccagg caatagcctg tgcgcgctgg
    23701 cgatggctga gtgtctgcac agagaggcta tccagtagga tctcgccttt taataacggt
    23761 ataacgccaa gaatggagcg catgagtgtg gttttgccgc aaccattcgc gccaagcaga
    23821 caacaaattg ttccttgcga cagggtgaag gacacgtcgc gtagcactgg atggcgggag
    23881 taacccagag acgcatgacg aacggcaagc aaagtcactg gcgacctccg cgtaacaaca
    23941 aaagcaggaa aaacggtgcg ccaacaaaag cggtcagaat gccaagtggg atttctgttg
    24001 ttccaatgct gcgcgccagc gtatcggtaa gcaggagcag aatagcacca gtacacattg
    24061 ccataggcaa cagctgctga tggttatgac cggtcaacaa gcgggcaatg tgcggaacga
    24121 ccagaccgat ccaaccaatt atgcccgcta ttgctaccgt gctggcggtg attagtgtgg
    24181 cgcagacaat caatccaaag cgcagacgcg tcacgttcag tcccagcgaa cgggcttcgt
    24241 cgtcggaaag cgtgagtaaa ttcatgcgcc agcggagaaa aaacaggggc aaggagccta
    24301 tgagaatgat cggtgcagca tagcagagat cgcgtaaggt cacggtggac agtcccccca
    24361 gcaaccagaa ggtaattgac ggtaactggg tgtaaggatc ggccagcgtt ttaattagcg
    24421 atatccccgc tccgcacaac gttcctaacg caatgcctac cagcaccagg gttaagatcg
    24481 gatcgtggcg ggtcacccga cgcgtaatca accagacgcc cgcgaccacc atcagcccgc
    24541 cgcaaaatgc gtacagctgg atgtaaacaa tgggtaatcc ccacaggatt gccgcgcagg
    24601 caccaagtcc ggcaccggcg gagacgccta aaatatccgg tgaaactaag ggattacgca
    24661 acattccctg ataggtggtt ccggctcctg ccagcgctgc gcccagtaac agcgcagcaa
    24721 gaatacgcgg cacgcggatt tgccagaaga caatttgttc ctgcacagac tcattttctt
    24781 gacgaagaat tagcgccagc agttgctgca tatcgagatg ataagcccca cttacggcgg
    24841 caaaagtgat actgatagct gccagcagca aaatacatcc gtttacgatt ctcattagct
    24901 cgccaccaat ttttgatagt cggcatcact gagtgaacaa tgccagaaca gttgggcgta
    24961 atgctgcatg tcacttttaa actggcgatt gatggcggga tccagccagg cgtgaagtct
    25021 gcgtaatccc agaagacggt tgattcccgg cggggcatcg agccagccaa agggcaagcc
    25081 acttaaaaat aagatgcgtt gttccgcaac ggctttcacg ccctgccaga gaggatcacg
    25141 acgaataaaa tctgcagtaa cggcctcctg aaccagaata atatccggct gccagcgcag
    25201 gagattttcc atggaaacct gtgtcaggcc gtggcgatcg gctatttgcg cgacgttgtg
    25261 caaacctaat aattccgccg cctcggtatg caacgaaccc tgtaagcccg tttccagccc
    25321 tcggggaccg cgcgcagcat aaaagcggag gttagcggcg ggtgaggtgg cgaatgcctg
    25381 agcttcacca acgaagtggc tggcgagatt ggcttgttct gcggcgcggt gctcttcgcc
    25441 taacgttttg ccaagcgttg ttaactgttc tgctgattgc gccagttttc cgttaagcaa
    25501 taaccagggg atttgtgtct gttcgctaac ctgccgtgcc tgggagatca aggtttcatc
    25561 cgtattgccg caatcaacga ccaaatcggg atgtaacgcc ataagccctt ccagcgatag
    25621 tgtgctggcg cgcccggcga gtcgtcccag cctgggtaac tggcgaatgt gctctggcaa
    25681 tggaattaat gcctgacggg caaaatcaaa agaggaaaaa ccgaccattt tttctggcgc
    25741 gacagccagc aagagcaaat cggcgggcgc gcctgcgctg acgatgcgtg taatgttttg
    25801 ggcttcggga agagattgcg aagcaaaacg attaactgtt tgtgaaaagg acggtagaga
    25861 ctgcaggagc agggtagatg caagtgcctg cgcaaacgtc cgtctggtta tgggcatggt
    25921 gaacatcctg aaaagtaaaa tcgatatata ctttcatata tatcgataaa aatcatgttc
    25981 aatatcaaag tttagctatt gaaaacatga agatgtgacg acttccgcaa gcctgttgac
    26041 cctctctatc attaaaaatg tatttatcag gtggctaata ttatttaaat catttaaggc
    26101 taaatcattt aaatatgaat gacattattg ttttttatcg tccacggtta cgcgacgttg
    26161 aagttacttg ttttataatg agcgattttt gatatctcgc cgagaaaatt atcagcattc
    26221 tctcgtttca gtgtcgtgaa tatactgtta acggaggtga aaagaatgct gggggttata
    26281 caaaacctat gttgttgtcg tccggattca ccgcttgctg atagtaagca gcgtaaccag
    26341 aagttaccgc aacaagactt tgaggatttt aagaacgacg ttattaatgt cattaacaac
    26401 aatgaagata tttattttct tagagagagt aatttactga atgcgtttat tcggacgtgc
    26461 aaaaaaagag atgataacag ttacggaaat atgatattta cgctttattt tgataacgat
    26521 atctatggga acagaattat caaagcatcg ctgaacggcg gagagttgca gttattaagc
    26581 acagggtgta gcaatgataa cattctcc
//
LOCUS       DAJTRB010000059        25469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTRB010000059 DAJTRB010000000
VERSION     DAJTRB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.059"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..1332
                     /gene="yjeM"
                     /locus_tag="OO330_002542"
                     /pseudo
     CDS             1..1332
                     /gene="yjeM"
                     /locus_tag="OO330_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO330_002543"
     CDS             complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO330_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5883365.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO330_002544"
     CDS             complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO330_002544"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5883366.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO330_002545"
     CDS             complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO330_002545"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5883367.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7028..7573
                     /gene="orn"
                     /locus_tag="OO330_002546"
     CDS             7028..7573
                     /gene="orn"
                     /locus_tag="OO330_002546"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5883368.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7784..7859
                     /locus_tag="OO330_002547"
     tRNA            7784..7859
                     /locus_tag="OO330_002547"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7817..7819,aa:Gly,seq:gcc)
     gene            7895..7970
                     /locus_tag="OO330_002548"
     tRNA            7895..7970
                     /locus_tag="OO330_002548"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7928..7930,aa:Gly,seq:gcc)
     gene            8006..8081
                     /locus_tag="OO330_002549"
     tRNA            8006..8081
                     /locus_tag="OO330_002549"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8039..8041,aa:Gly,seq:gcc)
     gene            8314..8367
                     /locus_tag="OO330_002550"
     CDS             8314..8367
                     /locus_tag="OO330_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883369.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8350..9489)
                     /gene="queG"
                     /locus_tag="OO330_002551"
     CDS             complement(8350..9489)
                     /gene="queG"
                     /locus_tag="OO330_002551"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5883370.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9488..11035
                     /gene="nnr"
                     /locus_tag="OO330_002552"
     CDS             9488..11035
                     /gene="nnr"
                     /locus_tag="OO330_002552"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5883371.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            11007..11468
                     /gene="tsaE"
                     /locus_tag="OO330_002553"
     CDS             11007..11468
                     /gene="tsaE"
                     /locus_tag="OO330_002553"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5883372.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11487..12824
                     /gene="amiB"
                     /locus_tag="OO330_002554"
     CDS             11487..12824
                     /gene="amiB"
                     /locus_tag="OO330_002554"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5883373.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLCNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            12834..14681
                     /gene="mutL"
                     /locus_tag="OO330_002555"
     CDS             12834..14681
                     /gene="mutL"
                     /locus_tag="OO330_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5883374.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14674..15624
                     /gene="miaA"
                     /locus_tag="OO330_002556"
     CDS             14674..15624
                     /gene="miaA"
                     /locus_tag="OO330_002556"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5883375.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15710..16018
                     /gene="hfq"
                     /locus_tag="OO330_002557"
     CDS             15710..16018
                     /gene="hfq"
                     /locus_tag="OO330_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5883376.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16094..17374
                     /gene="hflX"
                     /locus_tag="OO330_002558"
     CDS             16094..17374
                     /gene="hflX"
                     /locus_tag="OO330_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5883377.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17460..18719
                     /gene="hflK"
                     /locus_tag="OO330_002559"
     CDS             17460..18719
                     /gene="hflK"
                     /locus_tag="OO330_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5883378.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18722..19726
                     /gene="hflC"
                     /locus_tag="OO330_002560"
     CDS             18722..19726
                     /gene="hflC"
                     /locus_tag="OO330_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5883379.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            19808..20005
                     /locus_tag="OO330_002561"
     CDS             19808..20005
                     /locus_tag="OO330_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5883380.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20109..21407
                     /gene="purA"
                     /locus_tag="OO330_002562"
     CDS             20109..21407
                     /gene="purA"
                     /locus_tag="OO330_002562"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5883381.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21612..22037
                     /gene="nsrR"
                     /locus_tag="OO330_002563"
     CDS             21612..22037
                     /gene="nsrR"
                     /locus_tag="OO330_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5883382.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22076..24517
                     /gene="rnr"
                     /locus_tag="OO330_002564"
     CDS             22076..24517
                     /gene="rnr"
                     /locus_tag="OO330_002564"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5883383.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24697..25428
                     /gene="rlmB"
                     /locus_tag="OO330_002565"
     CDS             24697..25428
                     /gene="rlmB"
                     /locus_tag="OO330_002565"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5883384.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         5948 a         6467 c         7151 g         5903 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcaccgtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgacgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa
      841 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
      901 atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg
      961 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
     1021 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca
     1081 cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg
     1141 acggtggttg tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg
     1201 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
     1261 ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta
     1321 gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag
     1381 gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc
     1441 tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg
     1501 acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag
     1561 acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat
     1621 cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg
     1681 cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt
     1741 gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact
     1801 gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga
     1861 ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc
     1921 ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg
     1981 gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat
     2041 aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag
     2101 ccactgcaat ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag
     2161 catcagcaga tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag
     2221 gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat
     2281 gataaatacc agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac
     2341 gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga
     2401 atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg
     2461 caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc
     2521 aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc
     2581 taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag
     2641 catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt
     2701 ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat
     2761 ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc
     2821 gctgccttct ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac
     2881 cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg
     2941 gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta
     3001 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
     3061 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
     3121 accatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
     3181 cgtcatccac agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
     3241 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
     3301 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
     3361 cggcaaaatt gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
     3421 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
     3481 catcgggcgc acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt
     3541 cagcgcatcc tccagttgcc cgttggagac tttcagcttg gtcagttcca atagtagcgt
     3601 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag
     3661 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
     3721 cggctgttta ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc
     3781 ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc
     3841 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc
     3901 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat
     3961 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
     4021 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
     4081 ctccgcctca agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc
     4141 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt
     4201 attggcagac agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc
     4261 agactgcaac gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag
     4321 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
     4381 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
     4441 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
     4501 cgacacgctg cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag
     4561 tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg
     4621 ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt
     4681 cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc
     4741 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
     4801 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
     4861 ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt
     4921 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
     4981 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
     5041 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
     5101 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
     5161 gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
     5221 aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc acgttctgag cggtgagatg
     5281 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
     5341 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
     5401 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
     5461 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
     5521 aggcatgacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
     5581 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
     5641 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
     5701 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
     5761 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
     5821 cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
     5881 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
     5941 acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat
     6001 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
     6061 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
     6121 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
     6181 ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt
     6241 ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa
     6301 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct
     6361 ggaaaccatc aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa
     6421 cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg
     6481 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
     6541 ggcggagaca atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa
     6601 atccggacgc gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
     6661 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
     6721 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
     6781 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
     6841 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
     6901 ctgctggcct ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat
     6961 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
     7021 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
     7081 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa
     7141 tattctggca gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat
     7201 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
     7261 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
     7321 tgcgggaaaa tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa
     7381 atacatgccg gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa
     7441 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
     7501 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
     7561 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
     7621 attttgacgc taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
     7681 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
     7741 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt
     7801 ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccaa
     7861 aatttaaaag tgctgtaagg catagaccac ccaagcggga atagctcagt tggtagagca
     7921 cgaccttgcc aaggtcgggg tcgcgagttc gagtctcgtt tcccgctcca aaatttgaaa
     7981 gtgctgtaag gcacagacca cccaagcggg aatagctcag ttggtagagc acgaccttgc
     8041 caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaattcttct ctcaataaaa
     8101 tatctacagc gacgcgatgc gttattgctg gtttttgttg tctctgacaa actcttgtaa
     8161 acagagttat ccacagcctc aggctgtaat cttaatttca aagaaacttc gcacggtgaa
     8221 tggtattttt taacctattg ataaataagt taaaaatcaa gattccgttt tgtcgagtcg
     8281 atcacttgac gattttattc gtcttgaatt gcaatgcgtt tttattttta ttcacaagct
     8341 gtggatgaat caggcgtcac gcggtaaccc tttttcaatc acccgaacca gacgctgttt
     8401 tttcggcaat tgcacttcga ctatgcacgc atttcgtctc tctatttgct gcgcaatcgc
     8461 ccacgctatg tgctcatcga gaagtgggtg ctcaccttta cgactttcca gcgctgtcaa
     8521 aatcgtttca tcccaagggg cattgcctaa tgccacggcg atattacgca gccagcgcag
     8581 gtgacctata cggcgaatcg ccgatccttc cgtgactttt aaaaacttct cttcgctcca
     8641 ggcaaataac tcaatgagtt ccggtgcgtg tagcggctta cgcgggctga aatcgtcttc
     8701 tgtagtgagt tgcgaatagc gattccacgg gcagataagc tggcagtcat cgcaaccgta
     8761 aatacggttt cccattaacg gtcgcaactc ttccgggagc gccccttcaa gttcgatggt
     8821 gagataagag atacagcggc gagcatcgac ggtatatggc tcgacgatgg caccggtcgg
     8881 gcaaatcgtc atacaggcca cgcatttgcc gcatccttcc tcgactggtt gatccacggg
     8941 cagcggaata tcgaccagca attcgcctaa aaagaagaac gaaccggcct cgcgattgag
     9001 gataagtgag tgcttacctg tccagccgag cccagctttt tcagctaacg ggcgctcgag
     9061 aataggcgca gaatcgacaa acggtctaaa attcagcgaa acacaatgtt gctgaatcat
     9121 ctcgcccagc tttttgagtc ggttgcgcag aagtttgtga tagtcacggc ccagcgcata
     9181 acggctaaca tagccgagtt tggggttttt cagcgtgctg gcaaatgcgg cgttagcagg
     9241 aaggtaattc atccgcacgc tgattacacg cagcgtaccg ggtaataact catgggggcg
     9301 agcgcgcagc ataccgtgac gtgccatcca gtccatttcg ccgtggtatt gtttgtccag
     9361 ccatgcttgc agtttgggct cggactcgct gagatcggta tcggtaatac ctacctgctg
     9421 aaagcccagt tccagccccc actgtttaat tttttgcgct aactgattga gatcgagggg
     9481 ctctgacatg acggaccata caatgaagaa aaaccccgta agtataccac acaccgtctg
     9541 gtacgccgac gatatccgcc gcggagaacg cgaggcggca gatgtgctgg ggctcacact
     9601 ctatgagctg atgcttcgcg ctggcgaggc cgcattccag gtgtgtcgtt cggcgtatcc
     9661 tgacgcccgc cactggctgg tgctgtgcgg tcatggtaat aacggcggcg atggctacgt
     9721 ggtcgcgcga ctggccaaag cggtcggcat tgaggtcacg ttgttggccc aggagagcga
     9781 caaaccgttg ccggaagagg ccgcgctggc acgcgaagca tggttaaacg cgggtggcga
     9841 gatccatgct tcgaatattg tctggcccga atcggtagat ctgattgttg atgcgctgct
     9901 cggtaccggc ttgcagcaag cgccccgcga atccattagc cagttaatcg accacgctaa
     9961 ttcccatcct gcgccgattg tggcggttga tatcccttcc ggcctgctgg ctgaaaccgg
    10021 cgctacgcca ggcgcagtga tcaacgccga tcacaccatc acttttattg cgctgaaact
    10081 aggcttgctc actggaaaag cgcgggatgt tacaggacaa ctgcattttg actcactggg
    10141 gctggatagc tggctggcag gtcaggagac gaaaattcag cggttttcgg cagaacaact
    10201 ttctcactgg ctgaaaccgc gtcgcccgac ttcgcataaa ggcgatcacg ggcggctggt
    10261 gattatcggt ggcgatcacg gcacggcggg ggctattcgt atgacggggg aagcggcgct
    10321 acgtgctggt gctggtttag tccgagtact gacccgcagt gagaacattg cgccgctgct
    10381 gactgcacga ccagaattga tggtgcatga actgactatg gactctctta ccgaaagcct
    10441 ggaatgggcc gatgtggtgg tgattggtcc cggtctgggc cagcaagagt gggggaaaaa
    10501 agccctgcaa aaagttgaga attttcgcaa accgatgttg tgggatgccg atgcattgaa
    10561 cctgctggca atcaatcccg ataagcgtca caatcgcgtg attacgccgc atcctagcga
    10621 ggccgcacgg ttgttaggct gttccgtcgc tgaaattgaa agtgaccgct tacattgcgc
    10681 caaacgtctg gtacaacgtt atggcggcgt agcggtgctg aaaggtgccg gaaccgtggt
    10741 cgccgcccat cctgacgctt taggcattat tgatgccgga aatgcaggca tggcgagcgg
    10801 cggcatgggc gatgtgctct ctggtattat tggcgcattg cttgggcaaa aactgtcgcc
    10861 gtatgatgca gcctgtgcag gctgtgtcgc gcacggtgcg gcagctgacg tactggcggc
    10921 gcgttttgga acgcgcggga tgctggcaac cgatctcttt tccacgctac agcgtattgt
    10981 taacccggaa gtgactgata aaaaccatga tgaatcgagt aattccgctc cctgatgagc
    11041 aggcaacatt agacctgggc gagcgggtag cgaaagcctg cgatggcgca accgtaatct
    11101 atctgtatgg cgatttaggc gcaggtaaaa ccacctttag ccggggcttt ttacaggctc
    11161 tgggtcatca gggtaatgtc aaaagcccca cttatacgct ggtcgaaccc tatacgctcg
    11221 acaacttaat ggtctatcac tttgatttgt accgccttgc cgatcccgag gagctggagt
    11281 ttatggggat ccgcgattat tttgccaacg atgccatctg cctggtggag tggccacaac
    11341 aaggtacagg tgttcttcct gacccggatg tcgaaataca cattgattat caggcacaag
    11401 gccgtgaggc gcgcgtgagt gcggtttcct ctgcgggtga attgttgctg gcgcgtttag
    11461 ccggttaacc tttgaaaggt ggcgggatga tgtatcgcat cagaaattgg ttggtagcga
    11521 cgctgctgct gctgtgcacg ccggtgggtg ccgcgacgct ctctgatatt caggtttcta
    11581 acggtaatca acaggcgcgg ataacgttga gttttattgg cgatcctgat tatgcgttta
    11641 gccatcaaag caaacgcacc gtggcgctcg atatcaaaca aacgggcgtg attcagggac
    11701 tgccgttgtt gttcagcggc aataatctgg tgaaggcgat tcgctctgga acgcctaaag
    11761 atgcacaaac gctacggctg gtggtcgatc ttaccgaaaa cggtaaaacc gaagcggtga
    11821 agcggcagaa tggcagcaat tacactgtcg tctttacgat taacgccgat gtgccgccac
    11881 cgcctcctcc gccgcccgtg gttgcgaaac gcgttgaaac gcctgcggtt gtcgcaccgc
    11941 gcgtcagcga accggcgcgc aatccgttta aaacggaaag taaccgcact acgggtgtta
    12001 tcagcagtaa tacggtaacg cgtccggcag cgcgcgcgac ggctaacact ggcgataaaa
    12061 ttatcatcgc tattgatgcc ggacacggcg gtcaggaccc tggcgctatc ggccccggtg
    12121 gtacgcggga gaaaaatgtc accatcgcca tcgcgcgtaa attgcgtact ttgctcaatg
    12181 acgatccgat gtttaaaggc gttttaaccc gtgacgggga ttactttatc tcggtgatgg
    12241 ggcgcagtga tgtggcacat aagcaaaacg ccaatttcct cgtgtcgatt cacgctgatg
    12301 ccgcaccgaa ccgtagtgcg actggcgctt ccgtatgggt gctctctaac cgtcgcgcca
    12361 acagtgaaat ggccagctgg ctggagcagt acgagaaact gtcggagctg ctgggtgggg
    12421 cgggtgatgt gctggcgaac agtcagtctg acccctattt aagccaggcg gtgctggatt
    12481 tacagttcgg tcattcccag cgggtagggt atgatgtagc gaccagtatg atcagtcagt
    12541 tgcaacgcat tggcgaaata cataaacgtc gaccagaaca cgccagcctt ggcgttctgc
    12601 gctcgccgga tatcccatca gtactggtcg aaaccggttt tatcagcaac aacagcgaag
    12661 aacgtttgct ggcgagcgac gattaccaac aacagctggc agaagccatt tacaaaggcc
    12721 tgtgcaatta cttccttgcg catccgatgc aatctgcgcc gcagggtgca acggcacaaa
    12781 ctgccagtac ggtgacgacg ccagatcgca cgctgccaaa ctaaggacga ttgatgccaa
    12841 ttcaggtctt accgccacaa ctggcgaacc agattgccgc aggtgaggtg gtcgagcgac
    12901 ctgcgtcggt agtcaaagaa ctagtggaaa acagcctcga tgcaggtgcg acgcgtatcg
    12961 atattgatat cgaacgcggt ggggcgaaac ttatccgcat tcgtgataac ggctgcggta
    13021 tcaaaaaaga tgagctggcg ctggcgctgg ctcgtcatgc caccagtaaa atcgcctctc
    13081 tggacgatct cgaagccatt atcagcctgg gctttcgcgg tgaggcgctg gcgagtatca
    13141 gttcggtttc ccgcctgacg ctcacttcac gcaccgcaga acagcaggaa gcctggcagg
    13201 cctatgccga aggacgcgac atggacgtga cggttaaacc ggcagcgcat ccggtgggaa
    13261 cgacgctgga ggtgctggat ctgttctaca acaccccggc gcggcgcaaa ttcctgcgca
    13321 ccgagaaaac cgaatttagc catattgatg aaatcatccg ccgcattgcg ctggcgcgtt
    13381 tcgacgtcac gatcaacctg tcgcataacg gtaaaattgt gcgtcagtac cgcgcagtgc
    13441 cggaaggcgg gcaaaaagaa cggcgcttag gcgcgatttg cggcaccgct tttctcgaac
    13501 aagcgctggc gattgaatgg caacacggcg atctcaccct gcgcggctgg gtggccgatc
    13561 caaatcacac tacgcccgca ctggcggaaa ttcagtattg ctacgtgaat ggtcgcatga
    13621 tgcgcgatcg cctgatcaat catgcgatcc gccaggcctg cgaagacaaa ctgggggccg
    13681 atcagcaacc ggcatttgtg ttgtatctgg agatcgaccc gcatcaggtg gacgtcaacg
    13741 tacaccccgc caaacacgaa gtgcgtttcc atcagtcgcg tctggtgcat gattttatct
    13801 atcagggcgt gctgagcgtg ctacaacagc aactggaaac gccgctaccg ctggacgatg
    13861 aaccacaacc cgcaccgcgt gccatcccgg aaaaccgcgt ggcggcgggg cgcaatcact
    13921 ttgcagaacc ggcagctcgt gagccggtag ctccgcgcta ctctcctgcg cccgcctcag
    13981 gcagtcgtcc ggctgccccc tggccgaatg cgcagccagg ctaccagaaa cagcaaggtg
    14041 aagtgtatcg ccagcttttg caaacgcccg cgccgatgca aaaaccaaaa gcgccggaac
    14101 cgcaggaacc tgcacttgcg gcgaacagtc agagttttgg tcgggtactg actatcgtcc
    14161 attccgactg tgcgttgctg gagcgcgatg gcaacatttc acttttatcc ttgccagtgg
    14221 cagaacgttg gctgcgtcag gcacaattga cgccgggtga agcgcccgtt tgcgcccagc
    14281 cgctgctgat tccgttgcgg ctaaaagttt ctggcgaaga aaaatcggca ttagaaaaag
    14341 cgcagtctgc cctagcggaa ttgggtattg atttccagtc agatgcacag catgtgacca
    14401 tcagggccgt gcctttaccc ttacgccaac aaaatttaca aatcttgatt cctgaactga
    14461 taggctacct ggcgaagcag tccgtattcg aacctggcaa tattgcgcag tggattgcac
    14521 gaaatctgat gagcgaacat gcgcagtggt caatggcaca ggccataacc ctgctggcgg
    14581 acgtggaacg gttatgtccg caacttgtga aaacgccgcc gggtggtctg ttacaatctg
    14641 ttgatttaca tccggcgata aaagccctga aagatgagtg atatcagtaa ggcgagcctg
    14701 cctaaggcga tttttttgat ggggccgacg gcctccggta aaacggcgtt agccattgag
    14761 ctgcgtaaaa ttttaccagt agagttgata agcgttgatt ctgcccttat ttacaaaggg
    14821 atggttatcg ggacggcgaa gccgaacgct gaagagttac tcgccgcgcc gcaccgattg
    14881 ctggatattc gcgatccgtc gcaggcttac tcggctgctg attttcgccg cgatgcgctg
    14941 gcggaaatgg ccgatatcac cgcggcgggg cggatcccac tgttagtggg cggtacgatg
    15001 ttgtatttca aggcattgct ggaagggttg tcgccgctac cgtcggcaga cccggaagtg
    15061 cgggccagaa ttgagcaaca ggcggcagag caaggttggg agtcattgca tcgtcaactt
    15121 caggaggtag atccggttgc ggcagcaagg attcatccaa atgatccaca aaggctttcc
    15181 cgggcactgg aagttttttt catttcgggt aaaactttaa cggaactgac gcaaacgtca
    15241 ggagacgctc taccgtatca ggtgcatcag ttcgccatcg ccccggcgag ccgtgaactg
    15301 ctccatcaac gcattgagca gcgttttcat cagatgttgg cttcaggttt tgaagcagaa
    15361 gtccgggcgc tttttgcccg aggagatttg catacggact tgccttccat tcgttgcgtg
    15421 ggttatcgcc agatgtggtc ttaccttgaa ggcgaaatct catacgatga aatggtttat
    15481 cgaggtgttt gcgccacgag acagttggcg aagcggcaga taacctggct gcgtggttgg
    15541 gaaggggttc actggcttga cagtgaaaaa ccagaacagg cgcgtgacga agtattacag
    15601 gttgttggtg ctatcgcagg ctgaatgtgt acaattgaga cgtatcgtgc gcaatttttt
    15661 cagaatcgaa aggttcaaag tacaaataag catataagga aaagagagaa tggctaaggg
    15721 gcaatcttta caagatccgt tcctgaacgc actgcgtcgg gaacgtgttc cagtttctat
    15781 ttatttggtg aatggtatta agctgcaagg gcaaatcgag tcttttgatc agttcgtgat
    15841 cctgttgaaa aacacggtca gccagatggt ttacaagcac gcgatttcta ctgttgtccc
    15901 atctcgcccg gtttctcatc acagtaacaa cgccggtggc ggtaccagca gtaactacca
    15961 tcatggtagc agcgcgcaga atacttccgc gcaacaggac agcgaagaaa ccgaataagg
    16021 tttcgggctg tttttttaca cggggagcca gcgatcctgc gttccccgct gatctattta
    16081 gagggttata cgcttgtttg accgttatga tgctggtgag caggcggtac tggtacacat
    16141 ctattttacg caagacaaag atatggaaga cctccaggag tttgaatctc tggtctcttc
    16201 cgccggtgtc gaagcattgc aggtgattac cggtagccgt aaagcgccgc acccaaagta
    16261 ttttgtaggt gaaggtaaag cagttgaaat tgcggaagct gtcaaagcga tgggtgcttc
    16321 ggtcgttctt tttgaccatg ccctgagccc ggcgcaagag cgtaacctgg agcgtttgtg
    16381 cgagtgtcgt gttatcgacc gcaccggcct tattttagat attttcgccc aacgtgcgcg
    16441 tacccatgag ggtaagttgc aggttgagct ggcgcagctg cgccatctgg ctacgcgcct
    16501 ggtgcgtggc tggacccacc ttgaaagaca gaaaggcggg ataggtttgc gtggtccggg
    16561 tgaaacccag ctcgaaaccg accgtcgttt gttgcgtaat cgcatcgtgc agatacagtc
    16621 gcgcctggaa agagttgaaa agcagcgtga gcaggggcgg caatcgcgta tcaaagccga
    16681 cgttcctact gtttcgctgg tgggatatac caacgccggt aaatctaccc ttttcaatcg
    16741 catcaccgaa gcgcgggtct acgcggcaga ccagttgttt gccaccctcg acccgacgtt
    16801 gcggcgtatt gacgttgcag atgtcggtga aaccgtactt gcagataccg tagggtttat
    16861 tcgccacctg ccgcacgatc tggtggcggc atttaaagcc acgttacaag agacgcggca
    16921 agccacatta ctgctgcacg tcattgatgc ggcggatgtg cgtgtacaag aaaacatcga
    16981 agcggtgaat acggttcttg aagagatcga cgctcacgag atcccaaccc tgctggtgat
    17041 gaacaagatc gatatgctgg aagatttcga accgcgtatt gatcgggacg aagagaacaa
    17101 accgatccgt gtctggcttt ccgcacagac cggagcgggg ataccacagc tttttcaggc
    17161 tttgacggag cggctttccg gcgaggtggc gcagcataca ttgcgtctgc caccgcagga
    17221 agggcgtctg aggagtcgtt tttatcagct tcaggcaata gaaaaagagt ggatggagga
    17281 ggacggcagc gtaagtctgc aagttcgtat gccgatcgtt gactggcgtc gcctctgtaa
    17341 acaagaaccg gcgttgatcg attacctgat ctaacggcgt agcgtctgaa gcgtggagtc
    17401 atatcctctg gcgtcgaaag acaacaggga tcaccgcata acaaatatgg agcacaaaca
    17461 tggcgtggaa tcagcccggt aataacggac aagaccgcga cccgtgggga agcagcaaac
    17521 ctggcggcaa ctctgaggga aatggaaaca aaggcggtcg cgatcaaggg ccacctgatt
    17581 tagatgatat cttccgcaaa ctgagcaaaa agctcggtgg tctgggcggc ggtaaaggca
    17641 ccggatctgg cggtggcagt tcatcgcaag gcccgcgccc gcagcttggc ggtcgtgtcg
    17701 ttaccatcgc agcggcagcg attgtcatta tctgggcggc cagtggtttc tataccatta
    17761 aagaagccga acgcggcgtg gtaacacgct ttggtaaatt cagccatctg gttgagccgg
    17821 gtctgaactg gaaaccgacg tttatcgacg aagtcaaacc ggtgaacgtg gaagccgtgc
    17881 gtgaactggc cgcttctggt gtgatgctga cgtcggacga gaacgtagtg cgcgttgaga
    17941 tgaacgtgca gtaccgcgtc accaatccgg aaaaatatct gtatagcgtg accagcccgg
    18001 atgacagcct gcgtcaggct accgacagcg ccctgcgtgg agttatcggt aaatacacca
    18061 tggaccgcat tctgacggaa ggtcgtaccg tgattcgtag cgatactcag cgcgaactgg
    18121 aagagacgat tcgtccgtat gacatgggta tcacgctgct ggacgtcaac ttccaggctg
    18181 ctcgtccgcc ggaagaagta aaagcggcgt ttgacgatgc gattgccgcg cgtgaaaacg
    18241 aacagcaata cattcgtgaa gcagaagcgt ataccaacga agttcagccg cgtgcgaacg
    18301 gtcaggcgca acgtatcctc gaagaggcgc gtgcgtacaa ggcccagacc atcctggaag
    18361 ctcagggtga agtggcgcgc tttgctaaac ttctgccgga atataaagcc gcgccggaaa
    18421 ttactcgcga gcgtctgtat atcgagacga tggaaaaagt gttgggtaac acccgcaaag
    18481 tgctggttaa cgataaaggt ggcaacctga tggttctgcc gttagaccag atgctgaaag
    18541 gtggtaacgc ccctgcggcg aagagcgata acggtgccag caatctgctg cgtctgccgc
    18601 cagcctcttc ctccacaacc agtggagcaa gcaacacgtc gtccaccagt cagggcgata
    18661 ttatggacca acgccgcgcc aacgcgcagc gtaacgacta ccagcgtcag ggggaataac
    18721 gatgcgtaag tcagttatcg cgattatcat catcgtgctg gtagtgcttt acatgtctgt
    18781 ctttgtcgtc aaagaaggtg agcgcggtat tacgctgcgt tttggtaagg tactgcgtga
    18841 cgatgacaac aaacctttgg tttatgagcc tggtctgcat ttcaagatac cgttcattga
    18901 aacggtgaaa atgctcgacg cacgtattca gaccatggac aaccaggccg accgctttgt
    18961 gaccaaagag aagaaagacc tgatcgtcga ctcttacatc aaatggcgca tcagcgattt
    19021 cagccgttac tacctggcaa cgggtggtgg cgacatttcg caagcggaag tgctgttgaa
    19081 acgtaagttc tctgaccgtc tgcgttctga aattggtcgc ctggacgtga aagatatcgt
    19141 caccgattcc cgtggtcgtc tgaccctcga agtacgtgac gcgctgaact ccggttctgc
    19201 gggtacagaa gatgaagtta ctaccccggc agcagataac gccattgccg aagcggcaga
    19261 gcgcgtaacg gctgagacga agggcaaagt tccggtcatc aacccgaaca gtatggcggc
    19321 gctgggtatt gaagttgtcg atgtgcgtat caagcagatc aacctgccga ccgaagtgtc
    19381 tgaagcgatc tacaaccgta tgcgcgccga gcgtgaagcg gtagcgcgtc gtcaccgttc
    19441 acaaggtcag gaagaagcgg aaaaactgcg cgcgactgcc gactatgaag tgaccagaac
    19501 gctggcagaa gctgagcgtc agggccgcat tatgcgtggt gaaggcgatg ccgaagcagc
    19561 caaactgttt gctgatgcat tcagtaaaga tccggacttc tacgcattca tccgtagcct
    19621 gcgtgcttat gagaacagct tctctggcaa tcaggacgtg atggtcatga gcccggatag
    19681 cgatttcttc cgctacatga agacgccgac ttccgcaacg cgttaatata acgactgcgg
    19741 tacaggtcaa taaagccacc gcatcctcag ggatgtcggt ggttttcttt ttctataagg
    19801 ataatgaatg aattcgacaa tctggctggc gcttgccctg gttttggtac tggaaggttt
    19861 agggccgatg ctttacccga aagcatggaa gaagatgatc tctgcgatga ccaatttgcc
    19921 cgataatatt ttacgtcgtt ttggcggtgg acttgtggtt gcgggcgttg tggtctacta
    19981 catgttgagg aaaacgattg gctgaacaaa aaacagactg atcgaggtca tttttgagtg
    20041 caaaaagtgc tgtaactctg aaaaagcgat ggtagaatcc atttttaagc aaacggtgat
    20101 tttgaaaaat gggtaacaac gtcgtcgtac tgggcaccca atggggtgac gaaggtaaag
    20161 gtaagatcgt cgatcttctg actgaacggg ctaaatatgt tgtacgctac cagggcggtc
    20221 acaacgcagg ccatactctc gtaatcaacg gtgaaaaaac cgttctccat cttattccat
    20281 caggtattct ccgcgagaat gtaaccagca tcatcggtaa cggtgttgtg ctgtctccgg
    20341 ccgcgctgat gaaagagatg aaagaactgg aagaccgtgg catccccgtt cgtgagcgtc
    20401 tgctgctgtc tgaagcatgt ccgctgatcc ttgattatca cgttgctctg gataacgcgc
    20461 gtgagaaagc gcgtggcgcg aaagcgatcg gcaccaccgg tcgtggtatc gggcctgctt
    20521 atgaagataa agtggcacgt cgcggtctgc gtgttggcga ccttttcgac aaagaaacct
    20581 tcgctgaaaa actgaaagaa gtgatggaat atcacaactt ccagttggtt aactactaca
    20641 aagctgaagc ggttgattac cagaaagttc tggatgatac gatggctgtt gccgacatcc
    20701 tgacttctat ggtggttgac gtttctgacc tgctcgacca ggcgcgtcag cgtggcgatt
    20761 tcgtcatgtt cgaaggtgcg cagggtacgc tgctggatat cgaccacggt acttatccgt
    20821 acgtaacttc ttccaacacc actgctggtg gcgtggcgac cggttccggc ctgggcccgc
    20881 gttatgttga ttacgttctg ggtatcctca aagcttactc aactcgtgta ggtgcaggtc
    20941 ctttcccggc cgaactgttt gatgaaactg gcgagttcct ctgcaagcag ggtaacgaat
    21001 tcggtgcaac tacgggtcgt cgtcgtcgta ccggctggct ggacaccgtt gccgttcgtc
    21061 gtgcggtaca gctgaactcc ctgtctggct tctgcctgac taaactggac gttctggatg
    21121 gcctgaaaga ggttaaactc tgcgtggctt accgtatgcc ggatggtcgc gaagtgacta
    21181 ccactccgct ggcagctgac gactggaaag gtgtagagcc gatttacgaa accatgccgg
    21241 gctggtctga atccactttc ggcgtgaaag atcgtagcgg cctgccgcag gcggcgctga
    21301 actacatcaa gcgtattgaa gagctgaccg gtgtgccgat cgatatcatc tctaccggtc
    21361 cggatcgtac tgaaaccatg attctgcgcg acccgttcga cgcgtaattc tggtacgcct
    21421 ggcagatatt ttgcctgccg ggctaacagt gtgatacatt gctgtgtcgg ttaagccatt
    21481 acgctatccg acacagtgtt aaatcctcgc ttttttcctt ccccgaactg aaataaatta
    21541 gcgacacagc ttgtggctgg tttatcatca atataaatgt attttttccc gatttccctt
    21601 ttgaggttga tgtgcagtta acgagtttca ctgattacgg attacgtgcg ctgatctaca
    21661 tggcgtcatt gccagaaggg cggatgacca gtatttctga agtgactgac gtctacggcg
    21721 tctcccgtaa tcatatggtc aaaataatca atcaacttag tcgtgccggc tacgtgactg
    21781 ctgttcgtgg aaaaaatggc ggcattcgcc tgggtaaacc ggcgagtgcg atacgtattg
    21841 gtgatgtggt gcgcgagctg gagcccttat cgctggtgaa ttgcagcagt gagttttgcc
    21901 acattacacc tgcctgtcgg ttgaaacagg cgctttctaa ggccgtgcaa agttttctta
    21961 cggaactgga taactacacg cttgccgatt tggttgaaga gaatcaaccg ctttataaat
    22021 tattgctggt ggagtgacga aaatcttcat cagagatgac aacggaggaa ccgagatgtc
    22081 acaagatcct ttccaggaac gcgaagctga aaaatacgcg aatcccatcc ctagtcggga
    22141 atttatcctc gaacatttaa ccaaacgtga aaaaccggcc agccgtgatg agctggcggt
    22201 agaactgcac attgaaggcg aagagcagct tgaaggcctg cgtcgccgcc tgcgcgcgat
    22261 ggagcgcgat ggtcaactgg tcttcactcg tcgtcagtgc tatgcgctgc cggaacgcct
    22321 cgacctggtg aaaggtaccg ttattggtca ccgtgatggc tacggctttc tgcgggttga
    22381 agggcgtaaa gatgatttgt atctctccag cgagcagatg aaaacctgca ttcatggcga
    22441 tcaggtgctg gcgcagccgc tgggtgctga ccgtaaaggt cgtcgtgaag cgcgtattgt
    22501 ccgcgtactg gtgccaaaaa ccagccagat tgttggtcgc tactttaccg aagcgggcgt
    22561 cggctttgtg gttcctgacg atagccgtct gagcttcgat atcttaatcc cgcccgatca
    22621 gatcatgggc gcgcggatgg gctttgtggt cgtagtcgaa ctgactcagc gtccgactcg
    22681 ccgcaccaaa gcggtgggta aaatcgtcga agtgctgggc gacaatatgg gcaccggcat
    22741 ggcggttgat atcgctctgc gtacccatga aattccgtat atctggccgc aggctgttga
    22801 gcaacaggtt gcggggctga aagaagaagt gccggaagaa gcaaaagcgg gccgtgtcga
    22861 tttgcgcgat ttaccgctgg tcaccattga tggcgaagac gcccgtgact ttgacgatgc
    22921 agtttactgc gagaaaaaac gcggcggcgg ctggcgttta tgggtcgcga ttgccgacgt
    22981 cagctactat gtgcgtccgc caacgccgct ggacagagaa gcgcgtaacc gtggcacgtc
    23041 ggtgtacttc ccttcgcagg ttatcccgat gctgccggaa gtgctctcta acggcctgtg
    23101 ttcgctcaac ccgcaggtag accgcctgtg tatggtgtgc gagatgacgg tttcgtcgaa
    23161 aggccgcctg acgggctaca aattctacga agcggtgatg agctctcacg cgcgtctgac
    23221 ctacaccaaa gtctggcata ttctgcaggg cgatcaggat ctgcgcgagc agtacgcccc
    23281 gctggttaag catctcgaag agttgcataa cctctataaa gtgctggata aagcccgtga
    23341 agaacgcggt gggatctcat ttgagagcga agaagcgaag ttcattttca acgctgaacg
    23401 ccgtattgaa cgtatcgaac agacccagcg taacgacgcg cacaaattaa ttgaagagtg
    23461 catgattctg gcgaatatct cggcggcgcg tttcgttgag aaagcgaaag aaccggcact
    23521 gttccgtatt cacgacaagc cgagcaccga agcgattacc tctttccgtt cagtgctggc
    23581 ggagctgggg ctggagctgc cgggtggtaa caagccggaa ccgcgtgact acgcggaact
    23641 gctggagtcg gttgccgacc gtcctgatgc agaaatgctg caaaccatgc tgctacgctc
    23701 gatgaaacag gcgatttacg atccagaaaa ccgtggtcac ttcggtctgg cattgcagtc
    23761 ctatgcgcac tttacttcgc cgattcgtcg ttatcctgac ctgacgctgc accgcgccat
    23821 taaatatctg ctggcgaaag agcaggggca tcagggcaac accactgaaa ccggcggcta
    23881 ccattattcg atggaagaga tgttgcaact gggtcagcac tgttcgatgg cggaacgtcg
    23941 tgccgacgaa gcaacgcgcg atgtcgctga ctggctgaag tgtgacttca tgctcgacca
    24001 ggtaggtaac gtctttaaag gcgtaatttc cagcgtcact ggctttggct tcttcgtccg
    24061 tctggacgac ttgttcattg atggtctggt ccatgtctct tcgctggaca atgactacta
    24121 tcgctttgac caggtagggc aacgcctgat gggggaatcc agcggccaga cttatcgcct
    24181 gggcgatcgc gtggaagttc gcgtcgaagc ggttaatatg gacgagcgca aaatcgactt
    24241 tagtctgatc tccagtgaac gcgcaccgcg caacgtcggt aaaacggcgc gcgagaaagc
    24301 gaaaaaaggc gatgcaggca aaaaaggcgg caagcgtcgt caggtcggta aaaaggtaaa
    24361 ctttgagcca gacagcgcct tccgcggtga gaaaaaaacg aagccgaaag cggcgaagaa
    24421 agacgcgaga aaagcgaaaa agccatcggc gaaaacgcag aaaatagccg cagcgaccaa
    24481 agcgaagcgt gcggcgaaga aaaaagtggc agagtgatca ataccctctt taaaagaaga
    24541 gggttagatt gctgacaaaa tgcgctttgt tcatgccgga tgcggcgtga acgccttatc
    24601 cggcctacat aatcacgcaa attcaatata ttgcagagat catgtaggcc ggataaacgt
    24661 agtgcatcag gcaaaacgta aacaacgagt acattaatga gcgaaatgat ttacggcatc
    24721 cacgcagtgc aggccctgct ggagcgcgcc cctgaacgtt ttcaggaagt ctttatttta
    24781 aaaggccgtg aagataaacg tctgttaccg ctgattcacg cccttgagtc tcagggcgtg
    24841 gttatccagt tggcaaaccg ccaatatctc gacgagaaaa gcgacggtgc cgtgcatcag
    24901 ggcattatcg cccgcgtgaa gccaggacgt cagtatcagg aaaacgatct gccggatctg
    24961 atcgcttcgc tcgatcaacc gttcctgctg atcctcgacg gcgtaaccga tccgcacaac
    25021 ctcggcgcgt gcctgcgtag tgcggacgcc gcaggcgttc atgcggtgat tgtgccgaaa
    25081 gatcgctccg cacagctcaa cgctacggcg aaaaaagtag cctgcggcgc ggcagaaagc
    25141 gttccgctga ttcgggtgac taaccttgcg cgcaccatgc gtatgttgca ggaagagaat
    25201 atctggatcg tcggtacggc aggcgaggcg gatcatacac tctatcagag caaaatgacc
    25261 ggacgcctgg cgctggtgat gggcgcggaa ggtgaaggta tgcgtcgcct gacccgtgaa
    25321 cattgcgatg agttgatcag catcccaatg gcaggaagtg tttcttccct gaacgtttcg
    25381 gttgcaaccg gaatttgctt atttgaagcg gttcgtcagc gcagctagtt cctctgaatt
    25441 atgaaaagcc atccagattt ggatggttt
//
LOCUS       DAJTRB010000060        24629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTRB010000060 DAJTRB010000000
VERSION     DAJTRB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.060"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..374)
                     /locus_tag="OO330_002566"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="OO330_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="OO330_002567"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="OO330_002567"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5883385.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="OO330_002568"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="OO330_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5883386.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="OO330_002569"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="OO330_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5883387.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKTTKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="OO330_002570"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="OO330_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5883388.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="OO330_002571"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="OO330_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5883389.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="OO330_002572"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="OO330_002572"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5883390.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="OO330_002573"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="OO330_002573"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5883391.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="OO330_002574"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="OO330_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5883392.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="OO330_002575"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="OO330_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5883393.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="OO330_002576"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="OO330_002576"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5883394.1"
                     /translation="MQDSILTTVVKDIDGEVTTLGKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVALGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="OO330_002577"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="OO330_002577"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5883395.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11200
                     /locus_tag="OO330_002578"
     CDS             10736..11200
                     /locus_tag="OO330_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5883396.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11447..12160
                     /gene="ydiV"
                     /locus_tag="OO330_002579"
     CDS             11447..12160
                     /gene="ydiV"
                     /locus_tag="OO330_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5883397.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12224..13660
                     /locus_tag="OO330_002580"
     CDS             12224..13660
                     /locus_tag="OO330_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5883398.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEVMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13664..13855)
                     /gene="hemP"
                     /locus_tag="OO330_002581"
     CDS             complement(13664..13855)
                     /gene="hemP"
                     /locus_tag="OO330_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5883399.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13987..15033)
                     /gene="aroH"
                     /locus_tag="OO330_002582"
     CDS             complement(13987..15033)
                     /gene="aroH"
                     /locus_tag="OO330_002582"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5883400.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15190..16023)
                     /gene="ppsR"
                     /locus_tag="OO330_002583"
     CDS             complement(15190..16023)
                     /gene="ppsR"
                     /locus_tag="OO330_002583"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5883401.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16355..18733
                     /gene="ppsA"
                     /locus_tag="OO330_002584"
     CDS             16355..18733
                     /gene="ppsA"
                     /locus_tag="OO330_002584"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5883402.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18790..20436)
                     /gene="fadK"
                     /locus_tag="OO330_002585"
     CDS             complement(18790..20436)
                     /gene="fadK"
                     /locus_tag="OO330_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5883403.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20493..20786)
                     /locus_tag="OO330_002586"
     CDS             complement(20493..20786)
                     /locus_tag="OO330_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5883404.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20783..22072)
                     /locus_tag="OO330_002587"
     CDS             complement(20783..22072)
                     /locus_tag="OO330_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5883405.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22128..23066)
                     /locus_tag="OO330_002588"
                     /pseudo
     CDS             complement(22128..23066)
                     /locus_tag="OO330_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23086..23850)
                     /locus_tag="OO330_002589"
     CDS             complement(23086..23850)
                     /locus_tag="OO330_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5883406.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24166..>24629
                     /locus_tag="OO330_002590"
     CDS             24166..>24629
                     /locus_tag="OO330_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5883407.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFA"
BASE COUNT         6097 a         5945 c         6478 g         6109 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aagggcttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaaacga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggt actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agaaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctgggg
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggcgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcacct ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg
    10921 ttcgggattt gtggttgtga cgatgcgcga tcgtttcgat ttgcatctgc cccgtgaaac
    10981 caaacaacaa gcctctattg gcacgcaaat tgataaagac gagttgctgc ctggtgacct
    11041 ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt acgataccaa
    11101 caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac ttgataatgt
    11161 ctattggcag aaaaatttct ggcaggcgag acgaatctag tatgtcgaaa aattgacggc
    11221 gatgatacat cgccgtcaat ttactcctga atacagtagt attttgcaga caaaaattcc
    11281 gtaaatggat tattcgttta taatcaggat aatctaaaaa aaggccccct gaaaaagtgg
    11341 ttaacatgga acaaatgaaa gttactcctt ctgtaattgt tatgtcttat ggcctcatcc
    11401 cttttagccg gatactgaaa aacatccttc gagagggacg gttaccatga agattttttt
    11461 ggagaatctt tatcattctg attgctattt cctgccgata agagataacc agcaactcct
    11521 tgtcggcgtt gagttaatta cccatttctc cagtgaagat ggcacggttc gtatcccgac
    11581 gagtcgggtt atagcgcaac ttaccgagga gcaacattgg caattatttt cagagcagtt
    11641 ggaattacta aaatcatgcc aacacttttt tattcaacat aaactctttg cctggcttaa
    11701 tttaacgcca caagtggcta cattattatt agacagggat aattacgcag gcgaactatt
    11761 aaaatatccg tttattgagc tgttgattaa tgagaattac ccgcatctca atgaaggaaa
    11821 agataatcgc gatcttttgt ccttatcgca aatgtatccc cttgtgttag gcaatttggg
    11881 agcaggtaat agcacaatga aagcggtttt tgatggtctt ttcacccgcg taatgctgga
    11941 gaaaggtttt attcagcaac aaatcacgca tcgctccttc gaaccgttta ttcgtgcgat
    12001 ccaggcgcaa atttcccctt gttgcaattg catcatcgcg gggggcattg atacgccaga
    12061 aatattggca cagattaccc ccttcgattt ccatgctctc cagggatgcc tgtggcctgc
    12121 cgttcctata aatcagataa cgacgttggt tcagcgataa ccctcctgtt tgctggtgtt
    12181 taagacgaga gtaaccgtct acactatcaa acagatagga tctatgaccc tgtcttttat
    12241 tacccgctgg cgcgatgaat tgccagaaac ctatacagca ctttccccta cgcctttaaa
    12301 taatgcccgg ctgatttggc ataataccga actcgctaac acgctgagta ttccatcgtc
    12361 gctgtttaaa aatgccgcag gtgtctgggg cggcgaaacc ctactgcctg gtatgtcacc
    12421 actggcacag gtttacagtg gtcatcagtt cgtcgtctgg gcgggccaac tgggtgatgg
    12481 gcgcggcatt ttacttggcg aacaactgct tgctgatggc actacaatgg actggcatct
    12541 gaaaggtgct ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg ttttacgttc
    12601 gacgatacga gaaagtctcg ccagtgaggt gatgcattat ctgggcattc cgacgacccg
    12661 cgcgttaagt atcgtcacca gcgattcgcc agtgtatcgg gaaacggtgg agccaggcgc
    12721 gatgctgatg cgtgtggcac caagtcatct gcgctttggt catttcgaac atttttacta
    12781 tcgccgcgag ccggaaaagg ttcgtcagtt ggctgacttt gccattcgtc attactggtc
    12841 acatcttgaa gacgatgagg acaaataccg tctctggttt aacgatgttg tcgcacgtac
    12901 cgcgtcgtta attgcccaat ggcagacggt cggctttgca catggggtga tgaataccga
    12961 caacatgtcg ctgctgggac tgacgcttga ttacgggccg tttggttttc ttgatgatta
    13021 cgaacccggt tttatttgta atcactcgga tcatcaaggg cgttatagct ttgataatca
    13081 acctgctgtc gcgttgtgga atttacagcg tctggcgcag acattgtcac catttgttgc
    13141 cgtagatgcc ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg
    13201 acaacggatg cggcagaaac tgggcttcat gacggagcaa aaagaggata acgcgctact
    13261 gaatgaatta ttcagtctga tggcgcgaga gcgcagcgat tatacccgca cattccgcat
    13321 gctgagtctg accgagcagc acagcgcggc gtcaccgcta cgtgaggagt ttattgatcg
    13381 tgcggcattt gatgactggt ttgcccgtta tcgggggcgt ttgcaacaag acgaggttag
    13441 cgatagtgag cgtcagcaac tgatgcaaag cgttaaccca gctctggtat tgcgcaactg
    13501 gttggcgcaa cgggcgattg aagcggcaga aaagggtgat atgatggaat tgcaccgcct
    13561 gcatgaggcg ttgcgaaatc ctttcagcga cagagatgat gactatgtca gtcgtccacc
    13621 tgactggggt aaacggctgg aagtcagctg ctcgagttaa aggctactta gttaacagca
    13681 gcttgccagc ctgagtttta cggagcagat attcttgccc gtcatgatcg ataatcagtt
    13741 tgccatccgg acctaacagt gtctggctgg taattcgccg gatgggctgc ggggaagcgg
    13801 tcttgtgatt ggcatccgtt tcaggcgtga gttttctgct atccgtataa cgcataaaaa
    13861 ttaataataa ttgatataac aatgattcct attatcaata ttatggcagg ccagagcaag
    13921 cagtaatgtg ttgagaagac aacaagttgc gcagaaatgc ccatcccatc aggaatgggc
    13981 acgcattcag aagcgggtat ctaccgcaaa ggcgagtttt tcgaccaggc gttcggtatc
    14041 ttcccagccc agacacgggt cggtaatgga ttgaccgtaa gtgagcggct gaccgccgac
    14101 gattttttgc gttccttcgc gcaggaaact ttccgccata attccagcaa tctccgtaga
    14161 gccattgcgg atttgctgac aaatatcctc acaaacttct aactggcgac ggtgctgctt
    14221 ctggcagtta ccgtggctga aatccaccac cagatgttca ggtaaatcaa actcgtgcag
    14281 cgtatcgcag gctgcggcga tatcatcggc atgataattc ggttttttgc cgccacgcat
    14341 aataatgtgg ccatacgggt tgccgctggt ctgatagatg gtcatctgac catttttgtc
    14401 tggcgagagg aacatatggc tggcgcgggc tgcgcggata gcatccacgg caatccgcgt
    14461 gttgccatcg gtaccatttt taaaacctac cggacaggag agtgctgaag ccatttcgcg
    14521 gtggatctga ctttcggtag tacgagcgcc aatcgcgccc caactgatta aatcagcaat
    14581 aaactgaccg gttaccatat cgaggaactc agtcgcggta gggacaccca gctcatttac
    14641 ctgtaaaagt aatttgcgcg ccagctccag accgtgattt acccgatagc tgccgtttaa
    14701 atccggatcg gagattagcc ctttccagcc gacaacagtt cgtggttttt caaaataggt
    14761 gcgcattacg atttccagcc gtgactggta ctggttgcgc agcgactgca gacgggtggc
    14821 gtactccatt gcagcggtga gatcgtggat cgagcagggg ccaataatga ccaacagtcg
    14881 cttatcttca ccattcagta ttttttcaat tctgcggcgg gagtcggtga catgggtggc
    14941 gacgccaggc gttacgggat accgtagcgc gagttcggcg ggcgttacca ggctctcaat
    15001 acgcgcagta cggagttcgt cagttctgtt cattacaagt ctcagaaatt tgttgcttct
    15061 acggcaggac taccggaaag tgatgcgcat cacgataaac caatcgcagc tgatttcaag
    15121 tgcgaggtgt gtcacacctc gccgggtggt tagcatgata acaaaaaaac aaaccaatgc
    15181 actagttctc tagtacattc ggcgactaag gcccatgata tcgaggatct tggtggcaat
    15241 ctcttctacc gaataattgg tactgttaat ccacgggatc tgatttttac ggtacaaggc
    15301 ttccacttcc gcgacttcca tcctgcactg acgaagcgag gcatagcgac tgttctcccg
    15361 acgttcctcg cgaatcgccg ccagacgttc cgggtcgata gtcaggccga acaatttatg
    15421 ctgaagcggt ttgagcgacg cgggtagcac cagattatcc atatcgtcgg caataaaggg
    15481 gtagtttgcc gcgcggatac caaattgcat tgccagatac agactggtgg gggttttacc
    15541 acagcgagaa acaccgagca ggatcacctg agcctgatcc agattgcgca acgaaatgcc
    15601 gtcatcgtgg gcgagggtgt aatcaatcgc cgcaatgcgc gcatcatatt tattgagatt
    15661 attagggtta aggccatggg tacgatgagc aatcggcgtt ggatccagtt tcatctcttg
    15721 ttgtagcggg gcaaccagcg cctgaacgat atcctggcaa aagccttcac tttgcaagat
    15781 gatggcgcga atctccggca acacgatgga gtagaagacc agcgggcgca cgcctgtctg
    15841 gtgataaatt gcgtcaatct gatccttcac tgcccgtgca cggctctcat tttcgacaaa
    15901 cggcagcgtg atgctgctga tagtgacggg aaattgtgac attactgcgt gtcctaatac
    15961 ctccgcagtt attgccgtac catcagaaat ataaaaaacg tggcgatcaa cagcattatc
    16021 cattttgttc ttcccgtgaa gcagacataa ttctatgaaa gcataaatta aaagcgcaca
    16081 gaagcgtagg acgtaatgtc tggtttataa aatgaacctt cattttattt tttataaaaa
    16141 cagcatttca tttttatggt ttcgtttata ccgatggttt atgtggaaat tgtcgaatag
    16201 agcaggtttg cgcaacgctg ggatcagtct taaaaagtaa aaaaatatat ttgcttgaac
    16261 gattcaccgt ttttttcatc cggttaaata tgcaaagata aatgcgcaga aatgtgtttc
    16321 tcaaaccgtt catttatcac aaaaggattg ttcgatgtcc aacaatggct cgtcaccgct
    16381 ggtgctttgg tataaccaac tcggcatgaa tgatgtagac agggttgggg gcaaaaatgc
    16441 ctccctgggt gaaatgatta ctaatctttc cggaatgggt gtttccgttc cgaatggttt
    16501 cgccacaacc gccgacgcgt ttaaccagtt tctggaccaa agcggcgtaa accagcgcat
    16561 ttatgaactg ctggataaaa cggatattga cgatgttact cagcttgcga aagcgggcgc
    16621 gcaaatccgc cagtggatta tcgacactcc cttccagcct gagctggaaa acgccatacg
    16681 cgaagcctat gcacagcttt ccgccgatga cgaaaacgcc tcttttgcgg tacgctcctc
    16741 cgccaccgca gaagacatgc cggacgcttc ctttgccggt cagcaggaaa ccttcctcaa
    16801 cgttcagggt tttgacgccg ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa
    16861 cgatcgcgcc atctcttatc gtgtgcacca gggttacgat caccgtggcg tggcgctctc
    16921 cgccggtgtt cagcggatgg tgcgctccga cctcgcatct tctggcgtga tgttctccat
    16981 tgataccgaa tccggctttg accaggtggt gtttatcact tccgcatggg gccttgggga
    17041 aatggtcgtg cagggtgcgg ttaacccgga tgagttttac gtgcataaac cgacactggc
    17101 ggcgaatcgc ccggctatcg tgcgccgcac catggggtcg aaaaaaatcc gcatggttta
    17161 cgcgccgacc caggagcacg gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg
    17221 tgacatcttc tcgctgacca acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat
    17281 tgagaaacac tacggtcgcc caatggatat tgagtgggcg aaagatggcc acaccggcaa
    17341 actgttcatt gtgcaggcgc gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg
    17401 ttatacgctg cattcacagg gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat
    17461 cggcgcgggt ccggtgaaag tcatccatga catcagcgaa atgaaccgca tcgaacctgg
    17521 cgacgtgctg gttactgaca tgaccgaccc ggactgggaa ccgatcatga agaaagcatc
    17581 tgccatcgtc accaaccgtg gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact
    17641 gggcattccg gcggtagtgg gctgtggaga tgcaacagaa cggatgaaag acggtgagaa
    17701 cgtcactgtt tcttgtgccg aaggtgatac cggttacgtc tatgcggagt tgctggaatt
    17761 tagcgtgaaa agctccagcg tagaaacgat gccggatctg ccgttgaaag tgatgatgaa
    17821 cgtcggtaac ccggaccgtg ctttcgactt cgcctgcctg ccgagcgaag gcgtgggcct
    17881 tgcgcgtctg gaatttatca tcaaccgtat gattggcgtc cacccacgcg cactgcttga
    17941 gtttgacgat caggaaccgc agttgcaaaa cgaaatccgc gagatgatga aaggttttga
    18001 ttctccgcgt gaattttacg ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc
    18061 gttttatccg aagcgcgtca ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa
    18121 cctggtcggt ggtgagcgtt acgagccaga tgaagagaac ccgatgctcg gcttccgtgg
    18181 cgcgggccgc tatgtttccg acagcttccg cgactgcttc gcactggagt gtgaagcagt
    18241 gaaacgtgtg cgcaacgaca tggggctgac caacgttgag atcatgatcc cgttcgtgcg
    18301 taccgtagat caggcgaaag cggtggttga agaactggcg cgtcaggggc tgaaacgtgg
    18361 cgagaacggg ctgaaaatca tcatgatgtg tgaaatcccg tccaacgcct tgctggccga
    18421 gcagttcctc gaatatttcg acggcttctc aattggctca aacgatatga cgcagctggc
    18481 gctcggtctg gaccgtgact ccggcgtggt gtctgaattg ttcgatgagc gcaacgatgc
    18541 ggtgaaagca ctgctgtcga tggctatccg tgccgcgaag aaacagggca aatatgtcgg
    18601 gatttgcggt cagggtccgt ccgaccacga agactttgcc gcatggttga tggaagaggg
    18661 gatcgatagc ctgtctctga acccggacac cgtggtgcaa acctggttaa gcctggctga
    18721 actgaagaaa taaaataaat ccccggcggc gttcagtcgc cggggttatg tgatccccga
    18781 agatgaaact tattcaatct cttcacagac atcctgcgtt aatcgccgta taatatcttt
    18841 tcttaacaaa aacttttgta ttttatctga ggcagtgcgc ggtagttttt cgattaccac
    18901 gatatgttca ggatatttat attttgcgac ccgtttacgg ctaaaaaaag ccgccatctc
    18961 ttccagcgat aatgaatgat gcggcgcttt cagcacgaca taagcgcatg atcgttcacc
    19021 taaacgttca tcgggcattg caaccacaca ggcatcgtga attttaggat gctgcaataa
    19081 aatatcttcc acttcacggc tgctaatatt ttcgccgccg cggacaataa tatctttttt
    19141 gcgtccggta atttttatat agccagcctc atccatacgg cagagatcgc cgctgtaata
    19201 ccagccttct tcatccagcg cacgggcggt taattcaggt tcatcaaaat accccataaa
    19261 cacattgggg ccacgcgatg cttcttcacc ttcgcaacct ggcggtaagg tcttgcgtgc
    19321 gtcatcgacc actttaatct ctacacctgc ggcagcgtaa ccatcggtat gcataaagcg
    19381 cgacaaagaa ttatcgagat tcaccaccgc atgcggcgaa ctttctgtgg aaccataaac
    19441 acttaataat ttaatgctgc gctgctggca ttcgcgcgcc acttttttgg ggattgtggt
    19501 accgccgcaa agaaagaagc gcagcgctga aaggtctgcg ggttgtttct ctagtaaatt
    19561 caaaagatca tagacaaacg gcgttgcgcc tagcatacag gtgcaacgct gctgctcaag
    19621 cagcgcgaga cacgcatcag gagtgaaaat atctaacaac acgctgcgag cgccaattaa
    19681 gaatggtgcc gttacgccat gcagaaagcc cgttgcgtga ccaagtggcg caggcatcat
    19741 aaagacatcc agccaggtca gattcagccg cgcgcaataa gcccgctcac tggcgagaat
    19801 attgttatgc gttagcatca cgccctttgg cagaccctcg gttccggagg taaacagcac
    19861 cgcagctaat tcatcgccgt gggtcgttat cgccgtggtc agtgggatat tgtcggcgat
    19921 aatctgactt aatgagaggg aagaggtggc gggagccagt ttgtccacgc cgacaatttg
    19981 ttgtagttgt ggaagctgat tttgcagcgg caggattaaa tctaccggac gcgtttgttt
    20041 aaacaacgtc ggtgcaaaga acatttttgc ctgacactta ttgagcaccc acaccagttc
    20101 tgcttcccgc caggaaggca acagcggcac ggaaaccgca ccgattttca ggcaggcaag
    20161 atagataacg gtaaattcac accagccagg cagttgaaat gcgatgcgat cgcctgattc
    20221 aatacccttc gctaacatcc agtttgccag acagctcgcg gcgtgatcga gcgcgctata
    20281 ggtgtacgat gcaccatgat tatcgaccac ggcaattttg tctggcatcg cacgagcggt
    20341 ctgctgccag taatcggcca gtgaagcatc gccccatagc ccttgctgac gatacgccgc
    20401 acgacgttgt tcgttaaacg ttaatgtcac tttcattttg gactctcgaa acagaacatc
    20461 aggcccgagt tgcgggcctg tgggatgcat gattagccgt agcgaaaatc aataccgaag
    20521 gtgcctgcgg gatattgcca ctgttcgaga atagtgttac cgcacagcac ccgacaggtg
    20581 ccgcactcca gacaaccggc gggatcaaaa tgaatgtttc ctacgtcatc ctgcttataa
    20641 agtccggcag ggcaggcttt cattaattta cggaattcat tgatatcggg attttccgtc
    20701 agaatgatat gcgggtggcc ctcatcaaca tggaatttat tgacgcctaa tttgatgtcg
    20761 acgttaaccg tagcgttctg gctcatagcg cggttgctcc cttaatgcca tctttcagca
    20821 agttgatcag cccaattttc ttcgcgtgtc ccatgatcat tttgcgtacc ggctggtttg
    20881 gtttgccgtc aatggtgaac atctcgttca tgatgtcggc taccattcgt gggtattggc
    20941 taaacaggcg cgggttttcc atcagcgccg ggatcttgcg aaaatgctgc atatcacgca
    21001 tgacgcagct ttgttccagc tcacgtttgt attgcgccag actgttggcg gagaaatccg
    21061 cgcgttcttt ggcggcgatc accgttgtgg cggcagcctg agccgatgca atggctaaat
    21121 ccatgccgcg gaccgtaaaa cccaaattca ggcagaagcc tgcggcgtca ccaacgatca
    21181 tcacgccctc gttaaccagt tgcggcacca ttgccagacc gccttccggc accatatgcg
    21241 cggaatattc aagcagtttg ccgccgctaa tcagcgggcg aatggcgggg tgttgtttaa
    21301 aatcttccag catttgcggc acgctttttt gcgcatgggc gatatcaccc aatccacaaa
    21361 ccagccccaa ggatatggaa tccttgttgg tatagagaaa tcccccgccc atcaggccgt
    21421 cagaagggga accggcaaac agccaggcgg caccttcctc gcccgtaata ttaaagcgat
    21481 cgttgatctg ttctggtgtg aggccaataa cctctttaac accaacagcg taatgatgcg
    21541 gatcggaagc gggaaccatt cccagcgagc ggccaagcat cgagttaacg ccatcagcca
    21601 gaatcaccac attcgcttcg agaatatcat ccccagcctg cacgccagtg accttgtttc
    21661 cttcacgaac cagcgcgtcg acgcgaacgc ctgggataaa ctgcgcgccc gcctgctcgg
    21721 cttgttccat caaccacggg tccagacgat tacgcaatac ggtataagat gcgtgttgag
    21781 gaacatctgg ttgctcgcgg tgaaaatcga gggtaacggc gctttcttcg gttaagaagg
    21841 agattttctc gcgtgtgacc ttgcgttcta ccggcgctga tactgcaaag cctggaatga
    21901 ttgcttcaag tgtgtgggca taaagacgcc cgccggtcat gtttttacat ccggcactgt
    21961 cgccgcgttc tatcaccagg acatccagcc cggctcgtgc catgaccagt gcggcaacgc
    22021 taccagcaac gcccgcaccg accacaatgg catcaaattt gtcatccgac atgcgttatg
    22081 ctcccttcct gctgcgttta cagcgggaga tgtggttgaa aggggaatta gcggctcaac
    22141 tggctaatca gggcagggac gactttgtag atatcgccca ccagaccgta gtcggcatag
    22201 ttgaagattg gcgcattttt atctttattg atggcgacaa tcacttttac gccgttgccg
    22261 ccaaccatat gttggatctg cccggagatc cccagcgtca ggtagagatc ggatttcagc
    22321 aacacgccgg agacaccgat ataacgttca cgctccatcc agttctcgcc ttcggcaatt
    22381 ggacgtgaac agccgacttc agcattcagc accgccgcca gttcgtggac cattttcagg
    22441 tcatcctgcg ccgccagacc acgaccgacg ccaaccacac gttttgcttt gctcaggtcc
    22501 acgctgcttg cggctttcgc acggcgttcg cgacagagaa tttcatgacg cggagcaaca
    22561 taaggtaccg tttctgtcgg gcactgatga gaggtatcac tggtgcacgg ttcctgaaca
    22621 ccgggtgcaa gggtaatgat cgccagcggg ctgttgatct tttcctaagc gaacgctaac
    22681 ccgccataca tccggtgttc ggcgcaaatg tgaccatcga caatatccac cgccgtggca
    22741 tcgttcacca gcgccgcatt cagttgcaca cttaaccgtg ctgccagagc tttaccacgt
    22801 ttcgtcgcgg ccaacagcaa catagcgggg tgtttatctt tcagcagggc agcaatgctt
    22861 tcggcgtaat tttcagtgcg ttgcagcgcg tcgttttgct caagaacata aatacatttt
    22921 ggaccataag gcataactgc ctgcgcctgg tcggtatttt gtacaatggc atacacctgt
    22981 tggccccatt gctgagcgcc gccaaacagt tcagcataac gttcaggatt atcgctaaag
    23041 acccagacgc tgtttaattg actcatcgtt tcttctccgt aggcttcagt tcagggcttt
    23101 cttcagatgt tccgccagct cggcaatggc ctccggcgaa tcgttatcga gaatgatgtg
    23161 cttacgttct gtttgcggcg gtacgcgaat gccgaccagt tcagcaagtg gcgcgctctg
    23221 gctccagtca atatcacttg cctgccactg atttaccggt tttttacccg cgccgagaat
    23281 ggctttcatc gaaggaatgc gtggcacgtt aatatcggag gtgacgcaga gcacggctgg
    23341 aacagagagt tcaataactt caacatcatc ttcaagcgtg cgttcaatca ccagtgtatt
    23401 gccctgacgc tgaatagcac tcactgcatt aatcaccgga agttgcagaa tttctccgac
    23461 cagcaagcca acctgctggg cataaaggtc gccggaacct tcaccaaaga tcagtaaatc
    23521 gaagccgatc ttttcaattg ctgccgccag cgcttttgcg gtatcgagag gcagtgcatg
    23581 ttcaagttgc gcatcctgca ccaaatacag gctgtgcggc ccgcgggata gcacgtcttt
    23641 gcgcactttc gagttctgca acaatgagcc gccaacggtc agcgcggcta tctcatcgtc
    23701 atctgttgcg agctggcttg cagcttcaat ggcattgaga tcgaactggc tgattttggc
    23761 gtcggcattg tcgaaattca gggtgtattc tggagtgaca acaatgtcct gttcttcagg
    23821 caccagctta aagcaggtta ttattttcat gatttctcct gtgagtcgca tacggaacca
    23881 tctccgatgg aaggtaaaaa tggcctgaaa atagtgtgcg atagcggtgc ggggggttcg
    23941 acggagcaag ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatt gctaaaacaa
    24001 aagtgcaatt tgcggcaata tagttattcc atgaccgtac aatcttgcat tcagtgtaaa
    24061 taatcttgtt tagctctcgc taaacgcggt tgtataaccg caatacacgc cgtattttat
    24121 ccggcatata ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt
    24181 tttgataatg ccagtgaaac gctgtttgtc gccggtaaaa cgccacggct ttcacgtttt
    24241 gcatttagcg atgatccaaa atgggagtct ggacatcacg ttcatgacaa tgaaaccgag
    24301 ctgatttacg tcaagaaagg ggttgcaagg tttaccatcg attcttcgtt atatgtcgca
    24361 catgcggatg acattgtggt gatagaacgc ggcaggctgc atgcggtggc ctctgacgtt
    24421 aacgatccgg caacgacgtg tacctgtgcg ctgtacggct ttcagtttca gggggctgag
    24481 gaaaatcagt tactgcaacc gcattcttgt ccggtaattg ccgcggggca gggaaaagaa
    24541 gtcataaaaa ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa aaatagccaa
    24601 acatcttcgt tatgggatgc atttgccta
//
LOCUS       DAJTRB010000061        24172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTRB010000061 DAJTRB010000000
VERSION     DAJTRB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.061"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(12..584)
                     /locus_tag="OO330_002591"
     CDS             complement(12..584)
                     /locus_tag="OO330_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5883408.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            802..1662
                     /gene="tesB"
                     /locus_tag="OO330_002592"
     CDS             802..1662
                     /gene="tesB"
                     /locus_tag="OO330_002592"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5883409.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1711..2997)
                     /gene="amtB"
                     /locus_tag="OO330_002593"
     CDS             complement(1711..2997)
                     /gene="amtB"
                     /locus_tag="OO330_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5883410.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MIIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3027..3365)
                     /gene="glnK"
                     /locus_tag="OO330_002594"
     CDS             complement(3027..3365)
                     /gene="glnK"
                     /locus_tag="OO330_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5883411.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3546..5327)
                     /locus_tag="OO330_002595"
     CDS             complement(3546..5327)
                     /locus_tag="OO330_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5883412.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5320..7092)
                     /locus_tag="OO330_002596"
     CDS             complement(5320..7092)
                     /locus_tag="OO330_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5883413.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7122..7580)
                     /gene="decR"
                     /locus_tag="OO330_002597"
     CDS             complement(7122..7580)
                     /gene="decR"
                     /locus_tag="OO330_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5883414.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7734..8552)
                     /gene="cof"
                     /locus_tag="OO330_002598"
     CDS             complement(7734..8552)
                     /gene="cof"
                     /locus_tag="OO330_002598"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5883415.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8651..10343
                     /locus_tag="OO330_002599"
                     /pseudo
     CDS             8651..10343
                     /locus_tag="OO330_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10408..11103
                     /gene="queC"
                     /locus_tag="OO330_002600"
     CDS             10408..11103
                     /gene="queC"
                     /locus_tag="OO330_002600"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5883416.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11155..11553)
                     /gene="fadM"
                     /locus_tag="OO330_002601"
     CDS             complement(11155..11553)
                     /gene="fadM"
                     /locus_tag="OO330_002601"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5883417.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11647..12018)
                     /locus_tag="OO330_002602"
     CDS             complement(11647..12018)
                     /locus_tag="OO330_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5883418.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12169..14040)
                     /gene="ppiD"
                     /locus_tag="OO330_002603"
     CDS             complement(12169..14040)
                     /gene="ppiD"
                     /locus_tag="OO330_002603"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5883419.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14232..14504)
                     /gene="hupB"
                     /locus_tag="OO330_002604"
     CDS             complement(14232..14504)
                     /gene="hupB"
                     /locus_tag="OO330_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5883420.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14713..17067)
                     /gene="lon"
                     /locus_tag="OO330_002605"
     CDS             complement(14713..17067)
                     /gene="lon"
                     /locus_tag="OO330_002605"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5883421.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     IYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17255..18529)
                     /gene="clpX"
                     /locus_tag="OO330_002606"
     CDS             complement(17255..18529)
                     /gene="clpX"
                     /locus_tag="OO330_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5883422.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18655..19278)
                     /gene="clpP"
                     /locus_tag="OO330_002607"
     CDS             complement(18655..19278)
                     /gene="clpP"
                     /locus_tag="OO330_002607"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5883423.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19525..20823)
                     /gene="tig"
                     /locus_tag="OO330_002608"
     CDS             complement(19525..20823)
                     /gene="tig"
                     /locus_tag="OO330_002608"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5883424.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21167..21484)
                     /gene="bolA"
                     /locus_tag="OO330_002609"
     CDS             complement(21167..21484)
                     /gene="bolA"
                     /locus_tag="OO330_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5883425.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21789..22367
                     /locus_tag="OO330_002610"
     CDS             21789..22367
                     /locus_tag="OO330_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5883426.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22411..23886
                     /gene="ampG"
                     /locus_tag="OO330_002611"
     CDS             22411..23886
                     /gene="ampG"
                     /locus_tag="OO330_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5883427.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6664 c         5818 g         6024 t
ORIGIN      
        1 gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt cacctgagtt
       61 ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg cacggcggtt
      121 tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat caccggctga
      181 acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact caacagaata
      241 cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa tggtgactgt
      301 ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc ggctaacgac
      361 gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt ctgacggatc
      421 cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc tgaaatagac
      481 gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca agccgccaac
      541 gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt ctccttatta
      601 tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa tagtaacgtc
      661 aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt cagactattt
      721 cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct tgctgcggca
      781 gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac attgttaaat
      841 ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg tttacgccag
      901 gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac cgtcccggaa
      961 gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag taagaaaccg
     1021 attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg ccgggttgct
     1081 gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc accagaagcg
     1141 ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct gccttcggaa
     1201 acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga taaattcatc
     1261 tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa aggtcacgtc
     1321 gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga tgacctgcgc
     1381 gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt agctctacag
     1441 ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga ccattccatg
     1501 tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga gagcacctcg
     1561 gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg cgtgctggtt
     1621 gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag ccggaggtga
     1681 aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct cgccgtggct
     1741 gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa ccgtcagatc
     1801 cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa tggcgatgct
     1861 ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc ccacgccgcc
     1921 cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac aaacgccgtg
     1981 cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca tggtaacgcc
     2041 ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa ccccaatgta
     2101 gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg ctcccagcag
     2161 agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg ccgccgccgt
     2221 tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc ccactgaccc
     2281 ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga ccatcggcag
     2341 gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca gatacgcgcc
     2401 caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga aatccagcgc
     2461 accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa tgtaagagag
     2521 cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt ccgccagcgc
     2581 cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta cgtggatata
     2641 ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca accagttaat
     2701 gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa ccacccagag
     2761 aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca cgtttttgcc
     2821 gcgaatcaac ccaccgtaaa acagggcaat ccccggaata atcataaaca gcaccagcgc
     2881 agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg cagccattac
     2941 cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta tcttcatttt
     3001 tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc gccggtacga
     3061 atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat ttttccggtg
     3121 taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc aatcgccaca
     3181 tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta cagctcggca
     3241 tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg aataccaatg
     3301 gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac ggtcaccagc
     3361 ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag aaggtattgc
     3421 aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa gattgaaaat
     3481 gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat gaaatattgc
     3541 gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc ttcgcccgca
     3601 agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg ctggtgagtg
     3661 ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc aataatagtt
     3721 gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc cagagcatgt
     3781 tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag gatcaggatt
     3841 tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc gactgagaga
     3901 ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg cgccagctcc
     3961 gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc ccgccccagc
     4021 gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac cattgccacg
     4081 ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag gtgaatctca
     4141 ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt accactgccg
     4201 gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat gttctttagc
     4261 accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt gccactctgt
     4321 aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc aaacacgcgc
     4381 tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc gattaagggt
     4441 tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc aatggtgccg
     4501 ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga aaacagactc
     4561 agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc catatagtgt
     4621 gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa ctgctggata
     4681 acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag ataggcgcgc
     4741 acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac cagcaccacc
     4801 gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat cgccaccagc
     4861 atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac gtagagatcg
     4921 cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg ttgggtatca
     4981 aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa ctgttgcact
     5041 gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc ggcggcaaac
     5101 agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg caggttattt
     5161 ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac ttctgccgcc
     5221 gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg cgaaccatac
     5281 gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga cggcctcctc
     5341 gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat acatatcgcg
     5401 ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga tatgtccgtg
     5461 ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg cactgatgat
     5521 taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct ctgtgcgtcc
     5581 gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta ataacgctcg
     5641 agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct cgcccacttc
     5701 tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta accgcgcgac
     5761 atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat tcgccacggt
     5821 gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc agctatcgag
     5881 ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga cgtcgaaatg
     5941 acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc aaatacccag
     6001 catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt cagtctgcgg
     6061 atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca cgggttcgct
     6121 accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt atgcagcact
     6181 accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa tcatcagacc
     6241 taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca ccaccatcca
     6301 actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga tggtcgggtc
     6361 gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt cggcatccgc
     6421 ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc ggatactggt
     6481 gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca gcttaaagcg
     6541 ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca tcggtaacag
     6601 ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca cagcgcagcc
     6661 cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca cgacgcgatc
     6721 gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt aaaactccgg
     6781 gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca gttggtaaga
     6841 cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa cggcaatcag
     6901 caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt ctgtcacgcc
     6961 atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga taatgacaag
     7021 caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc ttaattgagc
     7081 aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat gggtaaagaa
     7141 gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag ccccggcacg
     7201 ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac ctggacgcgc
     7261 atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg catttcggta
     7321 accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt tatcagcaca
     7381 aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt gccgataagg
     7441 ataccgtcgt cctccagccg tttcagacgc ttccagcagg gggtggttgt cagattaacg
     7501 gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc cagcagctta
     7561 cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt taattctatt
     7621 ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc acaaaatgac
     7681 aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc tcgttattcg
     7741 ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga gacagcctga
     7801 tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg cggcatcgca
     7861 ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt catcgcatca
     7921 ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac cgtcaatgca
     7981 gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga aaaacacaaa
     8041 tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag atcgtcgtga
     8101 tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac atcgattatc
     8161 tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc tttcccggta
     8221 aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg ctgatacagc
     8281 accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc ttccagagaa
     8341 tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc cccgagaata
     8401 tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc gcgttcacgc
     8461 agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat caataaagtg
     8521 ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg acacggttaa
     8581 tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa taatattgtt
     8641 ccgggtttat atgcgattgc tcaaccgtct taaccagtat caacgtctgt ggcaaccttc
     8701 agccgcaaac cgtcaccgtc agcgaactgg ccgaacgctg tttttgcagc gaacgccatg
     8761 ttcgtacgct gttgcgtcag gcacaggagg cgggatggct ggagtggcag gcgcagtcag
     8821 gacgcggaaa gcgcggacaa ttacgctttc tggtcacgcc ggaatcgcta cgcaatgcga
     8881 tgatggaaca ggcactggaa accggaaagc agcaagatgt gctggagctg gcgcaactgg
     8941 ccccaggtga gctgcgcact ctgttacagc catttatggg cggacaatgg caaaacgata
     9001 cacccacgtt gcgtattccc tactatcgcc cgctcgaacc gctacaacca ggctttttgc
     9061 ccggccgtgc cgagcagcat ctcgccgggc agatattttc cggcctgacc cgcttcgata
     9121 ataatactca gcgcccgatt ggcgatttag cgcatcactg ggaaacctct actgacaggt
     9181 tacgctggga cttttatctt cgttcaaccc tacactggca taacggcgat gcagtaaaag
     9241 cctcacactt acaccagcga ttattgatgc tgttacaact gccagcactg gatcaattat
     9301 ttattagcgt gaagcgtatt gaagtcaccc atccgcagtg tctgaccttc tttttacatc
     9361 gccccgatta ctggcttgcg caccggctgg cgagctattg cagccatctg gcgcatccgc
     9421 aattcccact gatcggcact ggtccttttc gcttaacaca attcacagcg gagctggtgc
     9481 gcctggagag ccatgattat taccatttgc gtcatccgct gcttaaagcg gttgagtact
     9541 ggataactcc gccgcttttc gaaaaagatt tgggaaccag ttgtcggcat cccgtgcaaa
     9601 tcaccatcgg caaaccggag gagctgcaac gggtcagcca agtcagtagt ggcatcagtt
     9661 taggtttttg ctatttaacg ttgcgcaaaa gtccccgact ctccctctgg caggcgcgaa
     9721 aagtgatctc cattattcat caatccggtt tattacaaac gttagaagtc ggagaaaacc
     9781 tgatcaccgc cagtcatgca ttactgccag gctggactat tccgcactgg caagtaccgg
     9841 atgaagtcaa actaccgaaa accttgacgc tggtttatca cctaccgata gaacttcata
     9901 ccatggcaga acgcctacag gcgacactgg cagcggaagg ctgtgaactc acaattattt
     9961 ttcataacgc aaaaaactgg gacgacacga ccctactggc acacgcagac ctcatgatgg
    10021 gcgacagatt aattggcgaa gcaccggaat atactctgga gcaatggcta cgttgcgatc
    10081 cactatggcc acatgttttc gacgctccag catatgcaca tctgcaatcg acgctggacg
    10141 cggtgcaagt aatgcctgat gaggaaaacc gatttaatgc cctgaaagcg gtttttagcc
    10201 agttaatggc agacgcgacg ctgacgccgc tgttcaacta tcactatcgc attagtgccc
    10261 ctcccggcgt gaacggtgtg cgactgacac cgcgcggctg gtttgaattt accgaagcct
    10321 ggcttcccgc gccgtcgcaa tgaatagctg gtccgggtta atcgcaggcg ttaccataac
    10381 gcttttattg ttcaagcagg attatctatg aaacgtgctg tcgttgtgtt cagtggaggc
    10441 caggattcca ccacctgtct ggtgcaggca ttacaacaat atgatgaagt ccattgcgtg
    10501 acgttcgatt acggtcagcg acatcgcgca gaaatcgacg tggcacgcga actggcgctg
    10561 aaactgggag cacgcgcgca taaggttctt gatgtaacgc tgctcaacga gctggcggtc
    10621 agcagcctga cacgtgacag cattccggtg cctgattatg aacctgaagc cgatggcatc
    10681 ccgaatacgt ttgtcccagg gcgtaatatt ttgttcctga cgctggcggc aatatatgcg
    10741 tatcaggtaa aagcagaagc cgtaattacc ggcgtctgtg aaacggattt ctccggctac
    10801 ccggattgcc gcgatgagtt tgtgaaagca ctaaaccatg ccgtcagttt gggcatggcg
    10861 aaagatattc gttttgaaac gccgctgatg tggattgata aagcggaaac ctgggcgctg
    10921 gcagattatt acggcaaact ggatttagtc cgtaacgaaa cgttgacctg ctataacggc
    10981 attaaaggcg acggttgcgg tcattgtgcg gcatgtaatt tacgcgccaa cggtttgaat
    11041 cattatctgg ccgataaacc gacggtgatg gcagcgatga agcagaaaac cgggttgagg
    11101 taattattcc gggtgtcgcc ggatgcggct tgagcatccg gcaccacaaa acgtttactt
    11161 aaccatctgc tccagctttt cgcgcaattc cccttccaga gctaatgctt tctgcgtttt
    11221 aagatcaata caaacaaacg taataagcgc atccgctacc acctgccctt ccggctccag
    11281 tgtaatgacc tggtttaaga tgccgctttt accgtttaat tgctgcaact gactggtaat
    11341 agttaacagg tcgcttaata ccgctgggcg acgatagtta atattgatat tgaccacgac
    11401 gaaggcgatg ttatgggccg tcatccactg aaaactgtcg ctattttcca ccccatccca
    11461 gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg acgtgctggt aaacgtcgag
    11521 atgatatcca cgaactttga tttgtgtttg catagcgcaa taaccttacg ttgttgttat
    11581 aagtacagag gtcataactg gtatgacctc ttcagtctgg caaaaaattg ccactatgca
    11641 aattaattac agggttaata ccgccagatt acgttccacc agcgaattgc ccatccccgg
    11701 cacctgcttt agatcctcca ccgttttaaa cggaccgtac tcttcgcgat aactgacaat
    11761 cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg gctagctctt ccgcgctggc
    11821 attattaatg ctgacccggg tgccttcttc gtcactggct ttcgccggta ctgctgcttt
    11881 actttgtgct gcaggagctt ccgctttggt ttctaccgcc gtcggtttcg ccacagaagc
    11941 tgccgccagc gcgctatgag acattccggc acaggccagg gacagggtaa tgagcagtgc
    12001 tttaattccg tgtttcatac tgttttctcc ttgtttgtta acagtggaat caccttaacg
    12061 ggagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg aaaaggccgc gaaagcgacc
    12121 ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgg cttcaggatt attgctgttc
    12181 cagcgcatcg ccaattttga ttttcgcctc tttacgcagg ttactcatca gagcttcaaa
    12241 gacgatttgt gcgttgttct gggtgatacc ctgcaccatc gcttttttct gatcttccgg
    12301 cattgaacct tgtttcactt catccagcgc cagcagaacc acattacctt gcatatcggt
    12361 cgccataccg tagctcggtt tgtctttcgc tggcagtggc agtgcaaacg ccgcctggct
    12421 aatcgggtca cgaccggaac ggcttaaggt tttcggctcg ccaaatttca gaccggcagc
    12481 ctgcatagct tccgcacctt tgccggcttt caaatcaacc agcagtttct cagcatccac
    12541 tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc ttaacttgtt cctgaacatc
    12601 tgccaacggt ttcaccgctt ccggtttgtg ctcgctgacg cgcagcacga atgcgcggtc
    12661 gccgtctacg gtgatgatgt cagagttgat gcccgacgcg ccgttttcac ctaccagacc
    12721 gccgttaaag atagcgtcgg caaccggctt gaagttcaac tcttccggca ggttatcttt
    12781 gctgaaccaa cccgtctgag tggctttaac gccggcagct tgctctgcac cggccagaga
    12841 ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc agcgcatagt acgcatcgag
    12901 ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt acttcgtcta acgatttcac
    12961 tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa ccgaccgaag atttgatgac
    13021 accagacagt tggccttttt ctttcagacc agcatttttc agttcgtcgg ggatagtggc
    13081 atcttctaac caacccatat cgccgccgtt acgggcagag ataatatcgg cagatttttc
    13141 tttggctaat gcagcaaaat caccgccttt attcagctca tcaagtaccg ctttcgcttc
    13201 atcttcagtt ttggtctgga tgatgctgta gcgggtacgc tgcggctggg tgaattgatc
    13261 ctgatgctga tcgtagtagc tctggatatc cgcatcgcta accggttgct gcatcgttgc
    13321 ggcaaccagc ttgatgtaac tcacgcggaa ttgttccggc gtcatgaaat tgtttttgtt
    13381 ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc tgcttcgccg ccagcgcgtt
    13441 aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg accagtgccg ccagctcgtc
    13501 ggtttcacct ttcagcataa aatcggtacc ggcaacgccg ttaatcagct gttgggtagt
    13561 gagctggtta cgcagcgcct gggcgtactg atcggcggtc atccccatct ggttgaggat
    13621 accgttatag cggctgttat caaatttgcc gtcaacctgg aaggctgggg tcgcgaaaat
    13681 cgcctgttta acctgctcat cactgatacc cagtttcagc tcacgagcgt actgatccaa
    13741 cagcgcctcg tcgatcagac gattcagcac ctgttgacgc agggttttca tatagccttc
    13801 gttcgctgcc agctcggagt attgatcgcc cagctgttgc tgcatacgat tacgctcgct
    13861 gttaaaggcg ttctcgaact gcccacggct gatttcctgg tcattcactt ttgcggcgta
    13921 gttattgcct ccgccaatca ggtaaccact cacgccggtc aatatgaacg acacgataat
    13981 gataccgaaa ataatcttga gcacgagact gtttgcagcc gtgcgtaagc tgtccatcat
    14041 ggtgtaacaa cactccgctg tagatgactg tgtacctcac gcaacgtatc gatgcgcgca
    14101 aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc acaagacaca gaaagtcacc
    14161 gaatacaaat aaaaaaggca catcagtaga tgcgcccttg aacttcgtca catccccact
    14221 ggggacaacg cttagtttac cgcgtctttc agtgctttac ctgcacggaa gctcggtact
    14281 ttagcggcag cgatggtgat ctctttaccg gtctgcgggt tgcggccagt acgggcagca
    14341 cgctctttaa cggcaaaagt accaaaacct accagtgcta catcatcccc ttctttcaga
    14401 gattcagtta cggaagcaat aatagcatct aacgcacggc cagccgcagc tttagagata
    14461 tcagcccctg cagcaatctt gtcgatcaat tgagatttat tcactcttct cttcctcttt
    14521 ataatttata tcgcacttga atccttcaag gtacgaacgc gcagcagtta tatcaggcca
    14581 gccatcccct tacaacaccc cttaataagg gcaagcccga tccgctatct aacttagcga
    14641 gacaaaaaaa ggctggcaag cccgaattag cctgccagcc ctgtttttat tagtgcattt
    14701 tgcgcgaggt cactattttg cagtcacaac ctgcatgcca gacggttcat tttgcagcgc
    14761 cagagtcaga acttcctcaa tgcgcttcac aggatgaatg tccagatcgg caattacgtt
    14821 gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga attagcactg ttttaatccc
    14881 gccgcgatgc gctgccagga gtttttcttt caaaccaccg atcggcagta cctgaccacg
    14941 cagagtgatc tcaccggtca ttgccacatc ggcacgaacc gggttaccgg tcaggcaaga
    15001 aaccagcgcg gtgcacatag caataccggc actcggacca tctttcggcg tcgcaccttc
    15061 cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca gggttgatcc ccagtttttc
    15121 cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac tcctgcatca cttcgccgag
    15181 cgaaccggta tagatcagtt tgcctttacc cggaacacat gcggtttcaa tggtcagcaa
    15241 gtcaccgccc acttccgtcc acgccagacc gattacctga ccgacacggt tttcgttatc
    15301 agcgcgacca tagtcgaaac gctgaacacc gaggtagtca tgcaggttat cgccgttaat
    15361 ttcgatatgt tttaatgact tatcgagcag taactgctta accgctttgc ggcacagttt
    15421 ggagatttca cgctccagac cacgcacgcc cgcctcacgg gtgtagtaac gaataatgcc
    15481 gataatggcg ctatcgtcga cggtcagctc accttttttc agtgcattac gttcaatctg
    15541 cttcggcagc aggtgacgtt tggcgatgtt cagtttttca tcttcggtat aaccggagag
    15601 gcgaatcact tccatacgat ccagcagcgg tgccggaatg ttcatggagt tcgacgtcgc
    15661 gacaaacatc acgtcgctga gatcgtaatc cacttccagg tagtggtcgc tgaacgctac
    15721 gttctgctct ggatccagca cttcaagcag tgcagaggcc ggatcgcctc gcatgtcaga
    15781 agacattttg tcgatctcat cgagcaggaa cagcggattt ttcacgccca ctttcgccat
    15841 tttctggatc agtttacccg gcatagaacc gatgtaagta cggcggtgac cacggatttc
    15901 cgcttcatca cgcacgccgc ccagcgccat acggacatat ttacgcccgg tggctttggc
    15961 aatggactga ccaagagagg ttttacctac ccccggcggc cctaccaggc agaggatcgg
    16021 tcccttgatt ttgttgacac ggctttgaac cgcaaggtac tcaaggatgc gatctttcac
    16081 gcgctccaga ccataatggt cggtatcaag gatttcctgc gcctgacgca ggtctttttt
    16141 gaccttgctg cgcgcattcc acggtacctg taccatccag tcgatataac cacgcactac
    16201 ggtcgcttct gccgacatcg gagacatcat tttcagcttc tgcaactctg cttccgcttt
    16261 ctcttttgcc tctttcggca tcttcgccgc gtcgattttg cgcttcaggg cttcgttttc
    16321 gtccggcacg tcgtccattt caccgagttc tttctgaata gctttcattt gctcgttcag
    16381 atagtactca cgctgggatt tctccatctg ctttttaacg cggttgcgaa tgcgtttctc
    16441 aacctgcagc agatcgattt ccgattccat cattgccatc agatattcca gacgttcgtt
    16501 aacgtcggac atctccagaa cggactgttt gtcagccagt ttcagcggca tatgtgcagc
    16561 aatggtatcc gccagacgcg ccggatcgtc gatgctattc agcgacgtca gcacttctgg
    16621 tgggattttt ttgttcagct tgatgtagcc ttcgaactgg ctgattgcag tacgcaccag
    16681 cacttcctgt tcccgctcat caatggtcgg cgactccaga tactccgcct tcgcagaaaa
    16741 gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc tgtaacccct cgaccagcac
    16801 tttgacggtg ccgtcaggca gtttcagcat ctgcaatata gaggccacgg tcccgacggt
    16861 gaaaagatcg tttacacccg gctcatccgt tgaagcttct ttctgcgcga ccagcataat
    16921 ttttttatca tggtccatcg ccgcttccag acaacggata gatttttccc gcccgacaaa
    16981 taaggggatg accatgtgcg gataaaccac cacatcgcgc agcggcaata cggggatttc
    17041 aatgcgttca gaacgctcag gattcataga gctctctctt agtttaattt ccgccaggta
    17101 atcagatgac acgactgtgc ttcacgccat ctattaacat gtacgtcagt atatggggat
    17161 gtttccccca cattcaacgc cgagaataga ggaaaaatta aaggggagat aaaatccccc
    17221 ctttttggtt aactaattgt atgagaatgg ttaattattc accagatgcc tgttgcgctt
    17281 ccggcttacc ataaatcagc aacggtttgc tttgaccatc aattaccgac tcgtcgataa
    17341 ccactttttc gacgtcttcc atggacggca gatcgtacat ggtatcgagc agtgcggctt
    17401 ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc catcgctttc ttagcgatag
    17461 catccagcgc ctcgtcacgg aattccagat ccacgccttc cagattaaac agcgcctgat
    17521 actgcttggt cagggcgttt ttcggctctt tgaggatctg aatcagagct tcttcgctca
    17581 gttcattcaa cgttgctaca accggcagac gaccaatgaa ctcagggata agaccaaact
    17641 tgatcagatc ttccggttca acctgcgcca gcagctcgcc ttcgcttgct ttgtcggact
    17701 tcgcttttac cgtcgcgcca aaaccaatgc cggagccggt ttctacacgg tgggaaatca
    17761 ctttatccag accggcaaac gcaccgccac aaataaacag gatcttagag gtatcaacct
    17821 gcaagaattc ctgctgcgga tgtttacgcc caccttgcgg tggaacagca gctaccgtac
    17881 cttcgatcag tttcaacagt gcctgctgta ctccttcacc ggaaacgtct cgggtaatgg
    17941 acgggttgtc tgacttacga gaaatcttgt cgatttcatc gatgtagaca atcccgcgct
    18001 gcgctttctg tacgtcgtaa tcgcactttt gcaacagctt ctgaatgatg ttttcaacgt
    18061 cttcgcccac ataaccggct tcggtcagcg tggttgcgtc ggccatggtg aacgggacgt
    18121 ccagcaggcg cgccagcgtt tcagccagca gcgttttacc ggaaccggtc ggaccgatca
    18181 gcagaatgtt acttttgccc aactcgacac cattgctggt atcgccgttg cgcagacgtt
    18241 tgtaatggtt gtataccgcg accgccagca cttttttcgc ctgttcctgg ccgataacgt
    18301 aatcgtccag gtggttgcga atttcatgcg gcgtcggtag cgcactgcgt tcacgatgcg
    18361 gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca taaatcaaca cattcgtcgc
    18421 agatatacac ggatggaccg gcaatcagct tgcgcacttc atgctggctt ttgccgcaaa
    18481 aagagcaata cagcaatttg cctgagccat ctttgcgttt atctgtcatg ggtcaaaacc
    18541 tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc cattctcagg cgcaagccgc
    18601 ttacagcgtt gtgccgccct agataagtat agcggcacag ttgcgcctct ggcatcaatt
    18661 acgatgggtc agaatcgaat cgaccagacc gtattccacc gcttcagggg cggaaaggaa
    18721 gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat tgacccgtat gaagcgccat
    18781 aagttcattc atgcgccctt taactttcag aatttcacgg gcatgaattt cgatatcggt
    18841 cgcctggccc tggtagccgc ccaacggttg gtgaatcatc acgcgcgaat tcggcaggca
    18901 aaaacgttta ccttttgccc ctgcggtcaa caagaaagcg cccatcgagg ccgcctggcc
    18961 catacagatg gtgctaacat caggcttgat aaactgcatg gtgtcataga tagacatccc
    19021 ggcagtgatc accccgcctg gggagttaat gtacagatag atatcttttt ctgggttttc
    19081 cgcttccagg aacagcatct gcgccacaat caggttagcc atgtggtctt caacctggcc
    19141 agtcagaaaa atgacgcgtt ccttaagtag acgagaatag atatcaaaag agcgctcacc
    19201 gcgtgaggtc tgttcaatga ccatcggcac cagcgccata tggggtgcaa agttatctcg
    19261 ttcgccgctg tatgacattt ccgtctcctg gataaaattg aaaaaaacct gctgtaccga
    19321 ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg catcaccata
    19381 tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt tcgcacggct
    19441 ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc ggtgacgggc ctttgtgcga
    19501 atttagcgcg ttatgctgcg taaattacgc ctgctggttc atcagctcgt tgaaagtggt
    19561 ttctttttca gtcactttcg ctttcgccag tacagcttca acagcctgtt cttccagagc
    19621 aacattgcgc atgttgtcca tcagttcttt gtttttgctg tagaactcga taacttcttt
    19681 cggatcttcg tacgcagaag ccatctcttc gatcaggcct ttcacgcgct cttcgtcagc
    19741 tttcagctcg ttggtgcgga taacttcgcc cagcagcagg ccaacaacta cgcggcgttt
    19801 agcctgttct tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt tgccaccgaa
    19861 acgctgtgca gcctggcgac gcagaacgtc gatttcgctg tcgatcagcg cagccggtac
    19921 gtcgatgtcg ttagctttta ccagaccttc gatcgcctga gacttaacgc ggttacggat
    19981 ggcgctcttc agctcgcgct ccatgttttt acgcacttca gcgcgcagac cttctacgga
    20041 accatcttca acgccgaaac gtttgatgaa ttctgcagtc agttccggca gttcacgctc
    20101 ttcaactttc ttcaggttga tagcgaattt cgctgcttta cctttcaggt tttctgcgtg
    20161 gtattcttcc gggaaggtca cgtcgatggt gaactcttcg ccagctttgt ggcctttgat
    20221 accgtcttca aagcccggga tcatacgacc ctggcccatc gccagtacga aatcagacgc
    20281 tttaccgcct tcgaactctt cgccgtctac agaaccggtg aagtcgatgg ttacgcggtc
    20341 ttctgcttca acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac gcagagtatc
    20401 cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa cttcgatcgc
    20461 ttccagaccc tgcagttcaa cttccggata aacttcaaac tctacagagt aagtgaagtc
    20521 ttcacccagc ttgtattcgc ccggaacata agtcggtgcg ccagccggat tgattttttc
    20581 tttaatgatg gcgtcaatga agttacggct catcaggtca cccagaacgt cctggcgtac
    20641 agacgcgcca taacgctgag caacgatatt cattggcact ttgcctttgc ggaagccgtc
    20701 aatacgtact tttttcgcaa cgttgaccag ctcgctttta acagcggtct cgatgctgtc
    20761 agcagcgata gtaatcgtta cacggcggcc aaggccttga gtggtttcaa ctgaaacttg
    20821 catcttgtta cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca agcacaggtt
    20881 gttggcttcg cggaaccggg atgttcttat aatcaatcac attccctgtt accagaatca
    20941 tcccgaaagc attcaaatag gacgcggcat tatagcggca tcacttttat gagtcgagaa
    21001 cggttattgc gcgctgtcgc gtattttttc acaattcccg ggcaattttc gtctgaatgc
    21061 tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt tttcataaaa
    21121 tctttagcaa catactggaa aagcgccgac agttgcaaat gcgtttttac gcgatgcttc
    21181 ctgctccacg acagggagga gaggcaaaga cggtgtcctg caacccttcc cactccttaa
    21241 tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga gagttcctcc gctaaagtac
    21301 tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg ctgaccagca
    21361 caactttaaa atggctttca gagccggctg ggacattgtg acgatagctt tcatccacta
    21421 cttcgaggaa tacgggttgg aacgccgccc ttaatttttc ttctatccgc tcacgtatca
    21481 tcatgaaata tctcctccga caacgctgag atgtcatcca tccctttaaa tactagccgc
    21541 ttttaccgtt tccattgcag cttaacaaca aatatttagg tttcgtcctg gtttttccgt
    21601 caagcgtatg aagtttactg atttaaagat tgaaacacag gcctcgcatc agcgtttctt
    21661 ttaccagggg tgattatttc gtcaacatga tgaatttaca tgccttaccc acttcccctc
    21721 gccgttggca atgttatgat ggcgggaatt tttaccaaac acgcttaaga gtctctgaga
    21781 gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg cttgcaggat
    21841 gcgcaaaacc gccaacaact attgaagttt ccccgacgat tacactgcca cagcaggatc
    21901 caagcctgat gggcgtcacc gtaagcatta atggtgccga tcagcgtacc gatcaggcgc
    21961 tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc gctctgcgtt
    22021 tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg gttggtccga
    22081 atggcccggt taatctgcaa atcatcgtta gccaactgta tgctgacgtg tcccagggca
    22141 acgtgcgcta caacatcgct accaaagcgg atatcgccat catcgctacc gcgcagaatg
    22201 gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg ttccaggcct
    22261 ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac cgataccatc gctgatatgt
    22321 ctcaggacac cagcattcac gaattcatca agcagaacgc gcgttaattt ctgccctctg
    22381 gcccggtgca agccgggcct gtagacgccc atgtccagtc aatatttacg tatttttcaa
    22441 cagccgcgtt cagccatatt gctgatcctg ggttttgctt ccgggctacc gctcgccctg
    22501 acatccggca ccttacaggc ctggatgacg gtcgaaaata tcgatctcaa aaccattggt
    22561 ttcttctctc tggtaggcca ggcttacgtt tttaaattcc tctggtcacc gctgatggac
    22621 cgctacacgc ccccattttt tgggcggcgg cgcggttggc tgctcgccac gcaaatcctg
    22681 ttattagtcg ccattgcggc gatgggtttt ctcgaaccag gcacccaact ccgctggatg
    22741 gcggcgctgg cggtggtgat cgctttttgc tctgcctccc aggatattgt cttcgatgcg
    22801 tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat cagcgtgctg
    22861 ggttaccgtt tagggatgct ggtttccggc ggcctagccc tgtggctggc agataaatgg
    22921 ttgggctggc agggcatgta ctggttgatg gcggcgctgt tgatcccctg tattatcgcg
    22981 acgttgcttg caccagaacc aaccgacacc attcctgtgc ccaaaacgct ggaacaagcg
    23041 gttgttgcac ctctgcgaga tttctttggt cgcaataatg cctggcttat tttgcttctt
    23101 atcgtgctgt ataagctggg cgacgcattc gccatgagcc tgacaaccac gtttttgatt
    23161 cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtaa acaaaacgct tggcttactg
    23221 gcgaccattg ttggcgcatt gtacggtggg attttgatgc agcgcctgtc gctgttccgg
    23281 gcgctgctga ttttcggcat tttacaaggt gcgtctaacg ctggttactg gctgctgtcg
    23341 attactgata agcatctcta cagcatgggc gcagccgtct ttttcgaaaa cctctgtggc
    23401 gggatgggca catcagcctt tgtcgcgctg ttaatgacgc tgtgtaataa gtcattttcc
    23461 gctactcaat ttgccctgct ctcagcgctt tccgctgtag ggcgagttta tgtcggcccc
    23521 gtggcgggtt ggtttgttga agcacacggc tggtcgacat tctatctatt ctccgtcgcc
    23581 gctgccgtac cggggcttat tttgctgctg gtttgccgcc agacgcttga atatacacga
    23641 gtaaatgaca actttatctc ccgtaccgaa tatccggcag gttatgcctt tgccatgtgg
    23701 acactggcgg cgggcgtcag cctgttggcc gtgtggttac ttctgttgac gatggacgcg
    23761 ctggatttga cgcacttctc tttcctgcct gctctgctgg aagtcggggt tttagtcgcc
    23821 ctttctggcg tcgtgcttgg tggtttgctg gattatctgg cgctacgaaa aacgcatctg
    23881 acgtaatctg taaatattat ttagctgtag taatcactcg ccgtaattat tacggctaaa
    23941 taaacatcag catcgcttat taatatttgt tcttttgtgc ggcttagcgt ttggaaatta
    24001 ttgacgccat ttataaattc tatttttctt acacgattca gctaatgagt ctttattttc
    24061 tcaccaccca gttgtcactt taatgataat tatttgttaa ataattgttt tatttcgcat
    24121 tggttatacc aattgcccgc caagactcct tctgcactcc ccttttgtta ta
//
LOCUS       DAJTRB010000062        24089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTRB010000062 DAJTRB010000000
VERSION     DAJTRB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.062"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="OO330_002612"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="OO330_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5883428.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO330_002613"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="OO330_002613"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5883429.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="OO330_002614"
     CDS             1531..2478
                     /locus_tag="OO330_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5883430.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="OO330_002615"
     CDS             2548..3354
                     /locus_tag="OO330_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5883431.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="OO330_002616"
                     /pseudo
     CDS             3364..3771
                     /locus_tag="OO330_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3771..4265
                     /locus_tag="OO330_002617"
     CDS             3771..4265
                     /locus_tag="OO330_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5883432.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="OO330_002618"
     CDS             4331..5128
                     /locus_tag="OO330_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5883433.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="OO330_002619"
     CDS             5139..5927
                     /locus_tag="OO330_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5883434.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="OO330_002620"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="OO330_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5883435.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="OO330_002621"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="OO330_002621"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5883436.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="OO330_002622"
     CDS             complement(8370..10001)
                     /locus_tag="OO330_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5883437.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO330_002623"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="OO330_002623"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5883438.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGVDIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDLNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQSKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLRNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="OO330_002624"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="OO330_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5883439.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="OO330_002625"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="OO330_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO330_002626"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="OO330_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO330_002627"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="OO330_002627"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5883440.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO330_002628"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="OO330_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO330_002629"
     CDS             complement(22523..23452)
                     /gene="metA"
                     /locus_tag="OO330_002629"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5883441.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23609..24052
                     /locus_tag="OO330_002630"
     CDS             23609..24052
                     /locus_tag="OO330_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5883442.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5946 a         6150 c         6104 g         5889 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtagaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggcgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc gcacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gcggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcagg ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatagt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt ttctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttactct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcaggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctct aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccacg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 ctcaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttatcatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga taattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaacctaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaatc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccacaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgccag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacta ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ctgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc ccagggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
    22381 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
    22441 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
    22501 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
    22561 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
    22621 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
    22681 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
    22741 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
    22801 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
    22861 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
    22921 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
    22981 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
    23041 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
    23101 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
    23161 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
    23221 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
    23281 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
    23341 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
    23401 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
    23461 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
    23521 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
    23581 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
    23641 ggagggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
    23701 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
    23761 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
    23821 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
    23881 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
    23941 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
    24001 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcccc
    24061 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAJTRB010000063        22821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTRB010000063 DAJTRB010000000
VERSION     DAJTRB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.063"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO330_002631"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO330_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5883443.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO330_002632"
     CDS             1207..1869
                     /locus_tag="OO330_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5883444.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO330_002633"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO330_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5883445.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO330_002634"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO330_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO330_002635"
     CDS             2795..3100
                     /locus_tag="OO330_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5883446.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO330_002636"
     CDS             3103..3507
                     /locus_tag="OO330_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5883447.1"
                     /translation="MADTHHAQGPGKSFLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO330_002637"
     CDS             3497..3796
                     /locus_tag="OO330_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5883448.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO330_002638"
     CDS             3982..4278
                     /locus_tag="OO330_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5883449.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO330_002639"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO330_002639"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5883450.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO330_002640"
     CDS             5627..5995
                     /locus_tag="OO330_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5883451.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO330_002641"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO330_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5883452.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="OO330_002642"
     CDS             7203..7904
                     /locus_tag="OO330_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5883453.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="OO330_002643"
     CDS             7927..8091
                     /locus_tag="OO330_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883454.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="OO330_002644"
     CDS             complement(8303..9613)
                     /locus_tag="OO330_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5883455.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIASLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="OO330_002645"
     CDS             complement(9641..10972)
                     /locus_tag="OO330_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5883456.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO330_002646"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO330_002646"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5883457.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="OO330_002647"
     CDS             complement(12683..13072)
                     /locus_tag="OO330_002647"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5883458.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15275)
                     /gene="tdcE"
                     /locus_tag="OO330_002648"
     CDS             complement(13086..15275)
                     /gene="tdcE"
                     /locus_tag="OO330_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5883459.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIACCVSP
                     MVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLD
                     HFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSA
                     IKYARVKPIRDENGLAVDFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRN
                     AVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKL
                     PFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNR
                     EMLLDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15309..16517)
                     /gene="tdcD"
                     /locus_tag="OO330_002649"
     CDS             complement(15309..16517)
                     /gene="tdcD"
                     /locus_tag="OO330_002649"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5883460.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16543..17874)
                     /gene="tdcC"
                     /locus_tag="OO330_002650"
     CDS             complement(16543..17874)
                     /gene="tdcC"
                     /locus_tag="OO330_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5883461.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17896..18885)
                     /gene="tdcB"
                     /locus_tag="OO330_002651"
     CDS             complement(17896..18885)
                     /gene="tdcB"
                     /locus_tag="OO330_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5883462.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(18984..19922)
                     /gene="tdcA"
                     /locus_tag="OO330_002652"
     CDS             complement(18984..19922)
                     /gene="tdcA"
                     /locus_tag="OO330_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5883463.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20112..20456
                     /gene="tdcR"
                     /locus_tag="OO330_002653"
     CDS             20112..20456
                     /gene="tdcR"
                     /locus_tag="OO330_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5883464.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20712..21251
                     /locus_tag="OO330_002654"
     CDS             20712..21251
                     /locus_tag="OO330_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883465.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECINETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            21273..21620
                     /locus_tag="OO330_002655"
     CDS             21273..21620
                     /locus_tag="OO330_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883466.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21789..22439
                     /locus_tag="OO330_002656"
     CDS             21789..22439
                     /locus_tag="OO330_002656"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883467.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6153 a         5762 c         5254 g         5652 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag ctttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag caaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gctggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 caattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa tatattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatcg
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccaac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc
    14161 ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc
    14221 gtacgaccgt ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag
    14281 aacagcgaat caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag
    14341 tgatcgatca gttcctgtgc ctgctgctca ttgaggacgc cagctttaaa gtcgcgctca
    14401 atgtagatat ccaggaacga tgccgtgcgc cccagcgaca tcgcgccgcc attttgcgat
    14461 ttcactgccg ccagataagc gaagtagagc cactgcaccg cttcctgtgc attctgcgcc
    14521 gggcgagaga tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga
    14581 cgatgctctg ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc
    14641 agacgagact ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca
    14701 tacagcgcta cgcggcgata gtcaccgata atgcgcccac gaccatagcc atctggtaaa
    14761 ccggtcagca cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg
    14821 ccctggttat gggttttacg cagatcggta aacagatatt caaattcact gtccatttca
    14881 cggccatagg cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca
    14941 cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat
    15001 cccgcatcat gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg
    15061 gttgcgtttt cgatacggat gccttccatt accttttccc acaattccgt ggtggcaggc
    15121 gtcgcttcgg cgagaaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa
    15181 tcgcggacat taatttcgct tttccagtcc gtacctttaa agccaagcca tgcgtcagcg
    15241 tacagcttat cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt
    15301 caactaaatt atgcaaattc tgcgggcgcg ttaactttac ctaaatgaat ggcatccaaa
    15361 gcaatcattt tttcttcgtt agtcggaata acgacgcaaa tgacatgcgc attttcactg
    15421 gaaacaattc gctcgccaaa agagttagat cgattattca tttctgtatc aatctctacg
    15481 cctaatacag ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt
    15541 ccgccggtga atataattcc atccagccga cgtaatgaag ctgcatgtcc ggcaatatga
    15601 cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc
    15661 caggcttttt ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac
    15721 tctttattca ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag
    15781 gacatcgcac caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc
    15841 gtcattccca ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca
    15901 ttgccaagat gcgccacaac caggccggag tcatcttccg ccagattcag cagcgaatgt
    15961 gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac
    16021 tcttcataat atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg
    16081 aaactggtat caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca
    16141 ataccactta aatttgcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta
    16201 tcaatgactt catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg
    16261 cggtggccaa ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt
    16321 gccttcaatg caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg
    16381 gataagaatg cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacaa
    16441 tcgctggcat cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca
    16501 accggaaatt cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac
    16561 gatgttcagg atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg
    16621 gtatttcgcc agagacggcg ctttacggat agcatacatc ggcaacaggc acagcaggga
    16681 tgcgataatc ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc
    16741 aacaacccag gtggagccca tgatgaagat catgctgaga gtattcagtt tacccagcga
    16801 cactttggtt ttgtcacctt tataaccgaa cttcagaatc agaccattca agccttccag
    16861 cgtccccaga tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc
    16921 cgcatattcc agtgtaatcg cgaacgttgt tttcgtaccg gtcatggacg caaagtggtt
    16981 agccagataa gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga
    17041 cagagtaaac aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc
    17101 acgagaaatg atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata
    17161 ctcttcacgt ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac
    17221 catgatggaa atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag
    17281 cgaaccgagg tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga
    17341 aatcagcacc aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag
    17401 atccttacca aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc
    17461 gcgattcagc ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat
    17521 agtaacgcca taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac
    17581 cacgccgcca gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt
    17641 agagccagaa agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc
    17701 caataccaac atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa
    17761 cagcaccccg gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt
    17821 acgccaggac gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta
    17881 tcctcaacga attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc
    17941 gatattgccg ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt
    18001 accgcttaat aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg
    18061 ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc
    18121 gactaattca cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc
    18181 cgccagggtg ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat
    18241 gccgtgaacg ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat
    18301 tgccaccgca ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac
    18361 atcatagaga tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg
    18421 atcatcgtaa ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc
    18481 gatagtgtcg ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc
    18541 cgctactttg gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat
    18601 cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt
    18661 gcgtttttcc gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga
    18721 acccgtacgc tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa
    18781 atagttggag cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc
    18841 aataatatca tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg
    18901 cctgaattaa atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt
    18961 tatgtttaaa taaaaacaaa caactaacca acttctatta attgccttcg tctacaccca
    19021 ttataagatg aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga
    19081 taatttttcg accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata
    19141 gtaataaatt gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa
    19201 tcagcattga gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca
    19261 atactgatgc catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt
    19321 tgtggcaaca cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt
    19381 cgggacttac tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc
    19441 tgaagcttca tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt
    19501 gccggtaaga acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc
    19561 tctttgaact tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca
    19621 aatgagactt ccaccaccgc ttcagaagac ataccgctta tctcattaac catatttttc
    19681 atttcacggg taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt
    19741 acaccggtat ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg
    19801 attttactga cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca
    19861 cttctaatga cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc
    19921 ataacgacct acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaataaaca
    19981 ggatatgtgc gaccactcac aaattaactt tcattacttt ccaaggtatc gttattacaa
    20041 ttaaatatct ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt
    20101 atcaaaaaaa aatgacgggg ataaccatat tttatggcga taacatcatt cgttatgtgg
    20161 ttaatacaaa aaaggggctg agaccatatt tcaagcaatt accggataat taccaggcaa
    20221 aatttgagtt aaatttaatg agcaagttct caaattttat tataaataaa ccattttcag
    20281 cgataaatac tgcggcacgt cacatttttt cacgctattt gttggagaac aaacatttat
    20341 tttatcaata ttttaaaatt tcgaatacag gtattgatca tctcgaacaa ttgattaacg
    20401 tcaacttttt ctcttctgac aggacgtcat tttgtgaatg caatcgtttt ccataaattc
    20461 ttctcccctc ataggcgacg aatagcattt tgtgttgagg atcacaaaac gaataattgc
    20521 tggtcgccgc gataaggtca gacgaagtca acaaggggga ttttcactgt gctttacatc
    20581 agcgtaggac acagaatgtt gcttcgttta ctgtaacgcc gggtaaatga gcgttttttg
    20641 atagtgcgta agtgtcgtgc gaggaaaata agcatctatt attgtttgtc atgacgacaa
    20701 ggcaggcatc aatgaaaggc ttcccaattg cacatatttt tcacccttca atcccgccaa
    20761 tgcacgcagt ggttaacaat cacaatagaa atattgatta ttggacggta aaaagaaagt
    20821 ttgcagaaat tgtctccacc aatgacgtta ataaaattta cagtataagt aatgaactcc
    20881 ggagagtatt atctgcaatt actgcattga atttctatca aggcgatgtt ccttctgtca
    20941 tgatccgaat ccaaccggaa aatatgagtc cattcatcat agatatttct acaggagaac
    21001 atgatgatta tatcatacaa acattagatg taggcacttt tgcacctttt ggtgaacaat
    21061 gtacttgctc agccgtcaat aaaaaagagc tggaatgtat taatgagacg atttctaagt
    21121 attgcgcaaa gttcacccaa aaagaagcca tcttaacacc ccctaggcac tttaataaaa
    21181 cctgcattac ttcagattgt tggcaaattc tctttttttc acccgatcat tttaataatg
    21241 atttttattg attatttata aaggataaaa taatgttccc tgtttcatca attggtaatg
    21301 atattagtag tgatttagtc cgtcgaaaaa tgaatgacct tcctgaaagc cccataggaa
    21361 ataacctcga agcactagca ccaggtatag aaaaactaaa acagacctct attcaaatgg
    21421 tcactttact taacgcgtta caaccaagtg gaaaatgcat tatcactggt gattttcaaa
    21481 aagaattagc gtacttacaa aatgtaattc tttataatga ctcgtctctt cgtctggatt
    21541 ttttaggtta taacacccaa attattcaac gatcggacaa tacctgcgaa cttaccatta
    21601 atgaaccgtt aaaaaactag gaaatatcta caggcaatat caatgttaat tgcccattaa
    21661 aagatattta caatgaaatc aggaggttga acgtaatctt tagttgtggg actggagata
    21721 tagttgatct atcctctctg gacttacgta atgtcgattt agattattat gatttcacag
    21781 ataaacatat ggctaatact attctaaatc attttaaatt ggattcaaca aattttacta
    21841 atgccaacat gtttcaggtt aattttgtta gttcaacaca aaacgccaca atctcctggg
    21901 attatttact aaaaataacg cctgttttaa taagcattag cgatatgtat tctgaagaaa
    21961 aaatcaagtt tgtcgaaagt tgtttaaatg agcttggaga cattaccgaa gaacaattaa
    22021 aaattatgag atttgcaatt ataaaatcta taccaagggc aactcttaca gataaattag
    22081 aaaatgaatt aacaaaagaa atatataaaa gctcatcgaa aatcaacaat tgcttgaaca
    22141 gaattaaatt accagagatg aaagaattct catcaaaaaa aatatatgat tacatcgata
    22201 caatcattga agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta
    22261 attatactat ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa
    22321 agacgaaaat tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata
    22381 taactcagag aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa
    22441 tgcaatatag acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct
    22501 taccctgtgc accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca
    22561 aaaatgcctg atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg
    22621 cgtgcttttg taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc
    22681 aacaatctga aaccccccgg aacggtttat tacgtacatc aggtgaaact gaccgataag
    22741 ccgctttctt ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc
    22801 aaaaacccgc cgaagcgggt t
//
LOCUS       DAJTRB010000064        22401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTRB010000064 DAJTRB010000000
VERSION     DAJTRB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.064"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            82..2667
                     /locus_tag="OO330_002657"
     CDS             82..2667
                     /locus_tag="OO330_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5883468.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRS
                     SGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSL
                     KGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEAD
                     GSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTW
                     YGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLN
                     QLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGK
                     LQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRG
                     QPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETL
                     LDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGS
                     LVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRI
                     ALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIAT
                     LDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRM
                     DAICR"
     gene            2658..3641
                     /locus_tag="OO330_002658"
     CDS             2658..3641
                     /locus_tag="OO330_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5883469.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            3739..4281
                     /locus_tag="OO330_002659"
     CDS             3739..4281
                     /locus_tag="OO330_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5883470.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4289..4804
                     /locus_tag="OO330_002660"
                     /pseudo
     CDS             4289..4804
                     /locus_tag="OO330_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            4770..5508
                     /locus_tag="OO330_002661"
                     /pseudo
     CDS             4770..5508
                     /locus_tag="OO330_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            5619..6629
                     /gene="pyrD"
                     /locus_tag="OO330_002662"
     CDS             5619..6629
                     /gene="pyrD"
                     /locus_tag="OO330_002662"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5883471.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6803..7345
                     /gene="zapC"
                     /locus_tag="OO330_002663"
     CDS             6803..7345
                     /gene="zapC"
                     /locus_tag="OO330_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5883472.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7342..8451)
                     /gene="ycbX"
                     /locus_tag="OO330_002664"
     CDS             complement(7342..8451)
                     /gene="ycbX"
                     /locus_tag="OO330_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5883473.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8695..10803
                     /gene="rlmKL"
                     /locus_tag="OO330_002665"
     CDS             8695..10803
                     /gene="rlmKL"
                     /locus_tag="OO330_002665"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5883474.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10815..12722
                     /locus_tag="OO330_002666"
     CDS             10815..12722
                     /locus_tag="OO330_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5883475.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12852..14105
                     /gene="pqiA"
                     /locus_tag="OO330_002667"
     CDS             12852..14105
                     /gene="pqiA"
                     /locus_tag="OO330_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5883476.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14110..15750
                     /gene="pqiB"
                     /locus_tag="OO330_002668"
     CDS             14110..15750
                     /gene="pqiB"
                     /locus_tag="OO330_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5883477.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15747..16310
                     /gene="pqiC"
                     /locus_tag="OO330_002669"
     CDS             15747..16310
                     /gene="pqiC"
                     /locus_tag="OO330_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5883478.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16566..16733
                     /gene="rmf"
                     /locus_tag="OO330_002670"
     CDS             16566..16733
                     /gene="rmf"
                     /locus_tag="OO330_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5883479.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16803..17321)
                     /gene="fabA"
                     /locus_tag="OO330_002671"
     CDS             complement(16803..17321)
                     /gene="fabA"
                     /locus_tag="OO330_002671"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5883480.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17390..19150)
                     /locus_tag="OO330_002672"
     CDS             complement(17390..19150)
                     /locus_tag="OO330_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5883481.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19335..19787
                     /gene="matP"
                     /locus_tag="OO330_002673"
     CDS             19335..19787
                     /gene="matP"
                     /locus_tag="OO330_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5883482.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19863..20909)
                     /gene="ompA"
                     /locus_tag="OO330_002674"
     CDS             complement(19863..20909)
                     /gene="ompA"
                     /locus_tag="OO330_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5883483.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21266..21775)
                     /gene="sulA"
                     /locus_tag="OO330_002675"
     CDS             complement(21266..21775)
                     /gene="sulA"
                     /locus_tag="OO330_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5883484.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21994..>22401
                     /gene="sxy"
                     /locus_tag="OO330_002676"
     CDS             21994..>22401
                     /gene="sxy"
                     /locus_tag="OO330_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5883485.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5719 a         5254 c         5874 g         5554 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattta gcggggctaa cagtattcat agcagcgcag acagtgattc tggcgacagc
      661 tattttctga atttaaacag tggcgttaat ttaggcccat ggagattgcg caacaattca
      721 acatggagtc gcagtagtgg ccaaaccgca gaatggaaga atctcagcag ctatttgcag
      781 cgggcggtta ttccactaaa aggcgaactg accgtaggtg atgattatac tgcaggcgat
      841 tttttcgata gtgtcagctt tcgtggtgtg cagctggcgt cagatgacaa catgctgcca
      901 gacagcctga aagggtttgc gcctgtggtg cgtggtatcg ccaaaagcaa tgcccagata
      961 acgattaagc aaaatggtta caccatttac caaacttatg tatcgcctgg tgcttttgaa
     1021 attagtgatc tctattccac gtcgtcgagc ggtgatttgt tagttgaaat caaagaagcg
     1081 gacggcagcg tcaatagcta cagcgtaccg ttttccagcg tgccattact ccagcgtcag
     1141 gggcgaatca aatacgcggt gacactggcg aaatacagaa ccaatagtaa tgaacagcag
     1201 gagagcaaat ttgcccagac cacgttgcag tggggcggac cgtggggaac gacatggtat
     1261 ggtggtggac aatatgctga atattaccgt gccgccatgt ttggtctggg atttaacctt
     1321 ggcgatttcg gagcaatttc gttcgatgcg acccaggcga agagtacgct ggcagaccaa
     1381 agcgaacata aaggtcagtc atatcgtttt ctgtatgcca aaacgctcaa ccaattgggc
     1441 actaattttc aattgatggg ctatcgctat tcgacgtcgg gtttctacac cctttccgac
     1501 accatgtata aacatatgga tggctacgaa tttaatgacg gtgatgatga agatacgccg
     1561 atgtggtcgc gttattacaa tttgttttac accaaacgtg gcaaactgca ggtcaatatc
     1621 tcccagcaat taggcgagta cggttcgttt tatttaagtg gtagccagca aacttactgg
     1681 cataccgatc aacaggatcg gctattacag tttggctaca acacgcaaat taaagatctc
     1741 tcgctggggg tttcctggaa ctacagtaag tcccgtggtc aacctgatgc tgatcaggtg
     1801 tttgcactaa atttttccct gccgctcaat ctgttgctcc ccagaagtaa tgatagctat
     1861 accaggaaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa cgaagggcac
     1921 attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct gagctacagc
     1981 gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag catggactac
     2041 aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg cagtcaacaa
     2101 caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat taccctgggg
     2161 caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa tactcgtgtg
     2221 gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc ctatgccact
     2281 tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa cgtggatctt
     2341 gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc ggagttcaat
     2401 gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc gctgcgtttt
     2461 ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga tgatgatggc
     2521 tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg gggcgaagct
     2581 cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa ctctgcgatt
     2641 acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta ttactacgac
     2701 tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat atcgggcagt
     2761 gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa actctggatg
     2821 aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac gaatcgaatg
     2881 attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga tgggcattcg
     2941 ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat gactatcttg
     3001 ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct ttcgaaaacg
     3061 tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa cgtttaggtg
     3121 gccagggggc tagcggtaat agcggtagct tttcactgaa aatattgcag ccgttcgttg
     3181 gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac aacatacccg
     3241 caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg tctggtacga
     3301 tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt gatctgggcg
     3361 acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc agaacggcag
     3421 aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac gtcaatctga
     3481 gtctgacggc aacctcagac cccagctatc cccaggcgat taagacgtca cgtcctggcg
     3541 tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt gggacactac
     3601 cgctctctat tcctgatgat gcagacagta tccgcgaatg aatgtctatc cagtcagcac
     3661 gacaggcgta ccaccagaaa ccgggcgatt tgaagccacg gcaacggtga gaataaattt
     3721 tgattaacgc gtgaacgtat gaagaaaaaa acgatatatc agtgcgttat tttgttcttt
     3781 agccttctta acatccatgt cgggatggct gggcctgaac aagtcagtat gcatatttat
     3841 gggaatgtgg tcgatcaggg ctgtgatgtc gccaccaaaa gtgcattaca aaatattcat
     3901 attggtgatt ttaatatcag tgattttcag gccgcgaata ccgtaagcac tgctgctgat
     3961 ttgaatattg atatcaccgg ttgtgccgct ggtattactg gcgcggacgt cctttttagc
     4021 ggcgaggctg acacccttgc gccgacactg ctcaaactaa ctgacacagg cggaagcggt
     4081 ggtatggcaa cggggattgc cgtgcaaatt cttgatgcgc aaagtcagca agaaatcccg
     4141 ctcaatcagg tccagcctct tacgccctta aaagccgggg ataacacact caaatatcaa
     4201 cttcgttata agtccacaaa ggcgggagca acgggcggta atgcgacggc ggttctctat
     4261 tttgatctgg tttaccagtg aaggagtgat gttgaaacgg ataatctgga ttctgttctt
     4321 actgggatta acggggggct gtgagctatt tgcccatgat ggcacggtca acattagcgg
     4381 atcgtttcgc cgtaatacat gcgtgctggc acaggatagc aagcaaatca atgtgcagtt
     4441 aggcgatgtc tcgctgacag gtttttttca tgggaattat ggcccggaga aatctttcat
     4501 cattaatttg caggattgcg gaacagatgt cagcacggtt gatgtcacct tttcaggaac
     4561 ccccgacgga gtgcagagcg aaatgctgtc cattgaaagt ggtacggatg cagccagtgg
     4621 gctggcaatt gcgattctgg atgatgcgaa gatattgatt ccgcttaact aggcgagtaa
     4681 agactacagc ctacatagcg gtaaggtgcc attaactttt tatgcgcaat tgcgacctgt
     4741 caatagcgat gtgcagtcgg gtaaagtgaa tgccagcgcg acatttgtac ttcattatga
     4801 ctaatacgtg gaacagatta gcgctcttga ttttcgccgt tttatcgctg ctggtggcgg
     4861 gtgaattgca ggcaggagtg gtggtcggcg gaacgcgatt tatctttccg gcagacagag
     4921 aatcgatatc tattttactg actaatacca gtcaggaatc ttggcttatt aacagcaaaa
     4981 tcaatcgccc aacgcgttgg gccggggggg gaagcgtcga cagtgccagc actattactg
     5041 gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg cttgctgaga
     5101 acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag cattgccagc
     5161 gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc ggtatttaaa
     5221 ctgttctggc gacccgaagg tttgccgggc gacccgctgg aagcttatca acaattacgc
     5281 tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta cattaacctg
     5341 attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc gcctaaaagc
     5401 cagcgtcaga caaactggtg tcaggtaata gcacggtgtc atgtcgcctg gcggacgata
     5461 aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa caggttctga
     5521 aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact cgcacataat
     5581 acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc ttcgttcgta
     5641 aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag caattacgcc
     5701 gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta
     5761 actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt gataaagacg
     5821 gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc ggtaccgtca
     5881 cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta gatgccgaag
     5941 gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta gagaacgtaa
     6001 aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa gatacgccag
     6061 tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc tatgcgggat
     6121 atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg caatatggtg
     6181 aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg caagcgatgc
     6241 accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa gacgaattga
     6301 tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca accaatacca
     6361 ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt ggcttaagtg
     6421 gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta gaattaaacg
     6481 gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg cgtgaaaaga
     6541 ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa ggtccgccgc
     6601 tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt tataagataa
     6661 tcagggcttt attttcagcc ctggttgttt tatattcatc gttgttgctt atttagacat
     6721 tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt tgctgcggtt
     6781 ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta
     6841 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
     6901 ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt gtgttgatga
     6961 tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagacca
     7021 aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac cgcaaatgcc
     7081 gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg gcgatgcggc
     7141 ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg aatctggcga
     7201 aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
     7261 gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga atgttgacag
     7321 cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg gtacacagct
     7381 acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg tgacttcgcc
     7441 ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg
     7501 aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac gaaatgcctg
     7561 tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg cggtatcatc
     7621 agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca cctcatcgcc
     7681 aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
     7741 atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg
     7801 ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa
     7861 caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg acgcgccgga
     7921 aaccaccaga ttggtgcgga attgctccat ttttacactg gccggacaac gttgttggag
     7981 atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagagg
     8041 tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac
     8101 ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt
     8161 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
     8221 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt
     8281 aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
     8341 cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg taagaccaat
     8401 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
     8461 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
     8521 acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg ccctgggttg
     8581 tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc catgtccaga
     8641 ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat
     8701 tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaat
     8761 ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg
     8821 cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc
     8881 gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca
     8941 gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc
     9001 cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg
     9061 aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg
     9121 ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt acatctgcgt
     9181 ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgctattgtg
     9241 atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg
     9301 ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca ccgtgggcgt
     9361 tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt gaaagcggaa
     9421 gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac
     9481 agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg gattggtgaa
     9541 ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg
     9601 tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga accggcgctg
     9661 attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg ctggaatctc
     9721 tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc agacaaacag
     9781 tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt tgccgaaagc
     9841 acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct gcgtaagaac
     9901 ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg cctgtatgac
     9961 gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag
    10021 gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct gttcgatatt
    10081 atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct gaaaacccgt
    10141 gaacgccata agggcaaaaa ccagtaccag aaactgggcg agaagggcga gtttcttgaa
    10201 gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga tactggtctg
    10261 ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg caaagatttc
    10321 ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg cggtgcacgc
    10381 agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg caacctgcgt
    10441 ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg
    10501 cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt ctctaactca
    10561 aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat gaaagatttg
    10621 aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg tggcttccgt
    10681 atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg
    10741 ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc
    10801 tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg
    10861 cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg
    10921 gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg
    10981 atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa caggatccac
    11041 cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa gaacaagcgg
    11101 aatatctgaa acgctatcac gatatttcgc gcctggtgat gaacgacccg agcgagaaaa
    11161 atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg tggcagctgg
    11221 aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt gcgctgtcgt
    11281 cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg agcaatccgc
    11341 gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc gactggctgg
    11401 aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac cgttcgttta
    11461 tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg acctatccag
    11521 ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa gaactacaaa
    11581 atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgccag gggatcaaag
    11641 cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt cgcgaacgtg
    11701 gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg
    11761 gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag caactggtga
    11821 aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt ccgaatgggt
    11881 gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcggac agcgggcgta
    11941 ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg gaactggatc
    12001 ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg gttaatggca
    12061 agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa cgggcgatga
    12121 cgccagtacg tgcgttttct ggcggtgagc ggaaccgctt gctgctggcg cgtttgttcc
    12181 tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat gtcgaaacgc
    12241 tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg gttagccacg
    12301 atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc ggcggtaaaa
    12361 ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa tatgtggcac
    12421 tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca gaaactgtaa
    12481 aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag ctaccgcaat
    12541 tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg gatgcttcct
    12601 tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct gctgcagagc
    12661 aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa aatggtggct
    12721 gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg cctgatgaac
    12781 tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata gcgcatcatt
    12841 aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg
    12901 acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt ccccggtgtg
    12961 gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc tatgcgttgg
    13021 ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg aacgttgcag
    13081 gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct gaggactacg
    13141 ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa
    13201 ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag caactggcac
    13261 gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg ggtgtgctgg
    13321 tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc tttctcccct
    13381 ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt cgctggttat
    13441 gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc gtcacgggga
    13501 tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct gatgaacccg
    13561 tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg cagtggacac
    13621 tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg cccatcatgg
    13681 tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc attctgttat
    13741 ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt atggtgccaa
    13801 cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat ggcaagcgcg
    13861 acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc tggtcgatga
    13921 ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta
    13981 tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca atgttttctg
    14041 ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt
    14101 cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa gaactggtct
    14161 cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct tttttatcat
    14221 tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg aattgaaggt
    14281 ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag cgccacactg
    14341 gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat ggaaaaattg
    14401 ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga agggattagc
    14461 ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc gaaaggcagc
    14521 aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga tgcgaaaggt
    14581 atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga tccggtgctg
    14641 ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca aaaacgcaat
    14701 atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag caatgttcgc
    14761 ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg tgtggagatg
    14821 ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga aggtctggat
    14881 ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca gaagagcatt
    14941 caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga ttcggtacgc
    15001 ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac cgtaagcaaa
    15061 gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg tattccggta
    15121 ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt
    15181 gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg
    15241 gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc aataaccggt
    15301 attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggctt ggcgcaaatc
    15361 cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa tccgatgatt
    15421 gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg
    15481 ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc gacggatatg
    15541 cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg ctccgcagcc
    15601 tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg
    15661 gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa
    15721 gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt gcagcactgt
    15781 ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc
    15841 agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta gagcaggtca
    15901 ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat gtgaagtatg
    15961 tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc aacaccctgg
    16021 ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct ctgggaagcg
    16081 cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat ggcaaggtca
    16141 ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt ccgttccgtc
    16201 tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg gccggtgtct
    16261 ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa ttataaagat
    16321 ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt ctaacgtgcg
    16381 gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta gctcacaaat
    16441 atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta ttgcttaact
    16501 gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta tgagggaaac
    16561 gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt ggttatcagg
    16621 ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgagt caaaggtcac
    16681 aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc tgattctgtc
    16741 tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct gtctgatgat
    16801 aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc ggtatagatc
    16861 agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg acgattaaca
    16921 atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac ctgaccagtg
    16981 aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag ccagccgagg
    17041 tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat aaccggatcg
    17101 ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc tgcttcaaca
    17161 taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc tatcatcagc
    17221 atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg accagaggca
    17281 agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt aagccttatt
    17341 ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat cagttcggaa
    17401 taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc tgttgcgatg
    17461 cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg ccatcccaca
    17521 ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag atggtgaatt
    17581 tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga acgttagcag
    17641 tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata gcaaagaagc
    17701 cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga tcgactgaac
    17761 ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc agggcgcaga
    17821 gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca aatgtcagcg
    17881 atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac gcctgcatga
    17941 tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg atgtcggtga
    18001 attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg
    18061 gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg
    18121 tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg aggaaacctt
    18181 cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag gtgtcgccat
    18241 cacacaggga ggcgacctct ttacactggc ggaggatcca ctgcgggcta agcggaagtg
    18301 tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa gcatccgctc
    18361 ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac cactggctta
    18421 ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata gcctgctctg
    18481 aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct accagaatga
    18541 ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac tcatcaaacg
    18601 caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac agcagaggtt
    18661 gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga tgcaccagac
    18721 caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt tgctccgctt
    18781 ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct gcactcactg
    18841 cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc agttggcctg
    18901 catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca gactcttccg
    18961 gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa
    19021 attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct
    19081 gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat gcaagtttcg
    19141 taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg
    19201 cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa agctcacagg
    19261 atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag ttacacggta
    19321 acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga
    19381 agtatctggt gaagaagcat cgcgaagggg agttaattac ccgttacata gaagccagtg
    19441 ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg ctggtaaatg
    19501 gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag accattcggg
    19561 ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc
    19621 tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt aaaacgcttt
    19681 ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac gcgaataaaa
    19741 tgtcttctct taagcaggat ctgcaggcat tgctgggtaa ggaataactg acgaaagtca
    19801 gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta ccagacgata
    19861 acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat ctctacgcga
    19921 cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg
    19981 ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc cgggatacct
    20041 ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc ctggttgtaa
    20101 gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc tttcggatct
    20161 aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg tttcagggtt
    20221 gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt ctgtacttcc
    20281 ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg gtaggaaaca
    20341 cccaggctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc accgatgttg
    20401 ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta ctcaacacca
    20461 cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc accaggtacg
    20521 ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat gtccagatcg
    20581 tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt gtatgcgccg
    20641 ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat ttcaaaacca
    20701 acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt ttcgtgggtc
    20761 ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag tttagcacca
    20821 gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc agccagtgcc
    20881 actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc caaaatacgc
    20941 catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc tgctcaatat
    21001 taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa gtctacaacg
    21061 tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt cttgtataag
    21121 gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa accgcttcag
    21181 acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg acaaaaaaaa
    21241 gttctaggat taatcctaaa tttacttaat gatacaaatt agagtgaatt tttagcccgg
    21301 aaagttgtct cgttgtgtga gaggatgcgc ttaccggacg cataataaac cccatagcgt
    21361 taccttcatt tgccgcatca acaagttcag catgctcttc ttcagtcaaa tcatctgtca
    21421 accaaccgat caccacactg taattgcccg tgcgtaaagc gcgaaccatt gactccactg
    21481 tgtggcaagg ggagagctgg ctaatctgca ttactttcgt taagggtagc ccagatgcct
    21541 gaacccattc ccgactcagt ttttgttgcg gtgttaacca gagttgccag cgcgattgct
    21601 gaccgagttg ctgtaacaat ggcaacagta gaagttgcgt catcatgggc tgatcttcgc
    21661 gatagacaac ttcactgata agcccggctg tagtgttttc cgtagagaca cgcgcaattt
    21721 tacttgctgc ggatgagaac gacgaagaac gatgtgcata gcctgaagtg tacataatca
    21781 atccagcccc tgtgagttac tgtatggatg tgcagtacat ccagtgacaa caaagatcaa
    21841 ccctattttc ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa cataaattaa
    21901 tcttgcccct taagaataag ttgcctattt tcgtagttaa cggatccgtt aatgtgaatc
    21961 attcttttat gttatgattt taaaaggaat tttatgaaaa gcctctccta taagcggatc
    22021 tataaatcac aagaatacct ggcaacgttg ggcacaattg aataccgatc attgtttggc
    22081 agttacagcc tgaccgttga cgacacggtg tttgcgatgg tttctgatgg tgagttgtat
    22141 cttcgggctt gtgagcaaag tgcacagtac tgtgtaaaac atccgcctgt ctggctgaca
    22201 tataaaaagt gtggccgatc cgttaccctc aattactatc gggttgatga aagtctatgg
    22261 cgaaatcaac tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc gctgaaagag
    22321 aaaagcacgc gcaatacccg ggaaagactg aaagatttgc ccaatatgtc ttttcatctg
    22381 gaagcgattc tcggggaggt g
//
LOCUS       DAJTRB010000065        22271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTRB010000065 DAJTRB010000000
VERSION     DAJTRB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.065"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..1018)
                     /locus_tag="OO330_002677"
     CDS             complement(<1..1018)
                     /locus_tag="OO330_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5883486.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTEGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPI"
     gene            complement(1120..2100)
                     /locus_tag="OO330_002678"
     CDS             complement(1120..2100)
                     /locus_tag="OO330_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5883487.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2108..2818)
                     /locus_tag="OO330_002679"
     CDS             complement(2108..2818)
                     /locus_tag="OO330_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5883488.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3035..4858)
                     /gene="typA"
                     /locus_tag="OO330_002680"
     CDS             complement(3035..4858)
                     /gene="typA"
                     /locus_tag="OO330_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5883489.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PVVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5231..6640
                     /gene="glnA"
                     /locus_tag="OO330_002681"
     CDS             5231..6640
                     /gene="glnA"
                     /locus_tag="OO330_002681"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5883490.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6813..7862
                     /gene="glnL"
                     /locus_tag="OO330_002682"
     CDS             6813..7862
                     /gene="glnL"
                     /locus_tag="OO330_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5883491.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7874..9283
                     /gene="glnG"
                     /locus_tag="OO330_002683"
     CDS             7874..9283
                     /gene="glnG"
                     /locus_tag="OO330_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5883492.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9395..9505
                     /locus_tag="OO330_002684"
     CDS             9395..9505
                     /locus_tag="OO330_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5883493.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9734..11107)
                     /gene="hemN"
                     /locus_tag="OO330_002685"
     CDS             complement(9734..11107)
                     /gene="hemN"
                     /locus_tag="OO330_002685"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5883494.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11296..11805)
                     /gene="yihI"
                     /locus_tag="OO330_002686"
     CDS             complement(11296..11805)
                     /gene="yihI"
                     /locus_tag="OO330_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5883495.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12387..13019
                     /locus_tag="OO330_002687"
     CDS             12387..13019
                     /locus_tag="OO330_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5883496.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13401..16187)
                     /gene="polA"
                     /locus_tag="OO330_002688"
     CDS             complement(13401..16187)
                     /gene="polA"
                     /locus_tag="OO330_002688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5883497.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEEFTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16551..17483
                     /locus_tag="OO330_002689"
     CDS             16551..17483
                     /locus_tag="OO330_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5883498.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17524..18954)
                     /locus_tag="OO330_002690"
     CDS             complement(17524..18954)
                     /locus_tag="OO330_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5883499.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19109..19735)
                     /gene="dsbA"
                     /locus_tag="OO330_002691"
     CDS             complement(19109..19735)
                     /gene="dsbA"
                     /locus_tag="OO330_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5883500.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19752..20738)
                     /gene="srkA"
                     /locus_tag="OO330_002692"
     CDS             complement(19752..20738)
                     /gene="srkA"
                     /locus_tag="OO330_002692"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5883501.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20815..21084)
                     /locus_tag="OO330_002693"
     CDS             complement(20815..21084)
                     /locus_tag="OO330_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5883502.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21154..21738
                     /gene="mobA"
                     /locus_tag="OO330_002694"
     CDS             21154..21738
                     /gene="mobA"
                     /locus_tag="OO330_002694"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5883503.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNHAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21720..22247
                     /gene="mobB"
                     /locus_tag="OO330_002695"
     CDS             21720..22247
                     /gene="mobB"
                     /locus_tag="OO330_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5883504.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5509 a         5584 c         5333 g         5845 t
ORIGIN      
        1 cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag cccagcgtac
       61 atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac agtacgagcg
      121 cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc aggatcttat
      181 ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg ttaatgatgc
      241 cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat ggaatgaaga
      301 atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata agccagatgg
      361 ttttgtctct cagcaccgag gtcatactgg tgttttgcga tgctccgtct tcttttttca
      421 cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata atgcctacgg
      481 caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag ccactgccaa
      541 accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg ccacgccctg
      601 tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga cttaccgact
      661 tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc cagacaaaga
      721 ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct gtcgcaatga
      781 gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata gacagaaaaa
      841 agccctctgt ggtgacaaat gagtttaccg acatagcatt accaatttga ccattggtca
      901 aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt aatttataaa
      961 ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc gtgagcataa
     1021 catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc gaccattcat
     1081 atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca gaagatggtt
     1141 tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat taattgcatc
     1201 ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg gtgtttcact
     1261 catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga acgccgacgc
     1321 atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat catcgccaag
     1381 gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg aaatacaggc
     1441 tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat ggacctgggt
     1501 aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat tgatcatgtt
     1561 agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca gcgacaccag
     1621 ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc tgtcctgata
     1681 cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc gcaaattacc
     1741 ttcataacgt ggataaaaac gtacggacgt tgccccagtt ttcaacgcga tggcgacggc
     1801 ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa ggttcagacg
     1861 gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa gagaaaaacg
     1921 ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg cgatatcaag
     1981 taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat aagcatgtaa
     2041 ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta ttgtcaccat
     2101 aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat tcaatatcta
     2161 tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc gctggcatac
     2221 ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt ttgaaaatca
     2281 atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa aatgagtggt
     2341 tttcaagttg ttcaataaaa cccggggcaa cttcaggatt aatatatgtt tcgtgataga
     2401 aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca gttgatggcg
     2461 tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt ttctcggtaa
     2521 acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc gccgacagct
     2581 ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc ttacggaaga
     2641 ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt ttaatgccca
     2701 gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg atgccatctt
     2761 tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc tccgccattg
     2821 tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa aaagaaagca
     2881 attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac gtaatcacag
     2941 acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa aaacctgcca
     3001 tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt gcgcggttgg
     3061 cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag gtcggagtca
     3121 cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc atgcggatag
     3181 gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc agtttcttac
     3241 cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata atctgacctt
     3301 cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga ccgaacagcg
     3361 cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga cgctgaccca
     3421 cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga ccagtaccgg
     3481 aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg atcacgtagt
     3541 cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct ttacgttcgc
     3601 ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc acgttttcat
     3661 acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg gataccgcca
     3721 gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt tcaccacgac
     3781 cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca acgttgtgta
     3841 ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta ccttctttac
     3901 cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca tcaacggaga
     3961 gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg ttcagttcgc
     4021 ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg atacgttcca
     4081 gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct tcgctatcga
     4141 tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca atgccgataa
     4201 cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga ccgtcaaggt
     4261 caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc atgtcttccg
     4321 ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa acgaccggga
     4381 agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc tgatccacaa
     4441 cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc ggcttcaggc
     4501 cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg tcaaatgcgt
     4561 caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca ccaccgaagt
     4621 cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg atagcggtgt
     4681 ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc atcacgcgct
     4741 cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg tctaccaggg
     4801 tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt tcgatcacaa
     4861 ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc actacaaaac
     4921 aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat cgctttcacg
     4981 gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat gtttccattg
     5041 aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg atgttaagca
     5101 tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta tcttttttac
     5161 ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac caatccagga
     5221 gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg aagtgaagtt
     5281 tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta tccctgctca
     5341 tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct cgattggcgg
     5401 ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca ctgcagtgat
     5461 tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg aacctggcac
     5521 cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag attacctgcg
     5581 ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct tcctgttcga
     5641 tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg atatcgaagg
     5701 cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc cggcagtgaa
     5761 aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt ctgaaatgtg
     5821 tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag tagcgactgc
     5881 tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg acgaaattca
     5941 gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg cgacctttat
     6001 gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt ctctgtctaa
     6061 aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc aggcgctgta
     6121 ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa acccgaccac
     6181 caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg cttactctgc
     6241 gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag cacgtcgtat
     6301 cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg ccctgctgat
     6361 ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg acaaaaacct
     6421 gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct ctctggaaga
     6481 agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg tgttcacgga
     6541 tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg tgcgtatgac
     6601 tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt tgtactaccc
     6661 ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct gcatgggctt
     6721 ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct ataatgcact
     6781 aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag cccgatgctg
     6841 ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat ctggcgatcc
     6901 attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa ttgtttggta
     6961 cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg caagaaagtc
     7021 tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat ggtcgctcgc
     7081 atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg gagatggcac
     7141 cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa caagtagctg
     7201 cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc ggtttacgtg
     7261 gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa tataccaaag
     7321 tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg gggccgcagc
     7381 tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg gtaacgctgg
     7441 tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc agcctgccgg
     7501 aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg cgcaatgcgc
     7561 tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc gcgtttcaac
     7621 tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa gataacgggc
     7681 cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc ggccgcgaag
     7741 gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat tcaggcaaaa
     7801 ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct atcaggaaat
     7861 aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata gttccatccg
     7921 ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat ttgagaacgg
     7981 cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt cagatatccg
     8041 tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc atccaatgct
     8101 tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg cctatcaaca
     8161 aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg cgctggttga
     8221 gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc ttaacggccc
     8281 aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta ttatcggtcg
     8341 gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg gtaaagaact
     8401 ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta tcgcgctgaa
     8461 tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg agaaaggcgc
     8521 gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg gcggcacgtt
     8581 atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc tgcgcgtgct
     8641 ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg atgtgcggat
     8701 tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt tccgtgagga
     8761 tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg aacgtcggga
     8821 agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac tgggcgtaga
     8881 agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt ggccaggcaa
     8941 cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg ggcaggaagt
     9001 gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga gtacttcgca
     9061 aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag cgctgcgttc
     9121 cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt tactgacgac
     9181 cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg gctggggccg
     9241 caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg cttgtgtgta
     9301 aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc gataagttca
     9361 gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa ttaatctggt
     9421 atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg tgctgtgtgc
     9481 cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg agaaagtcgt
     9541 aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc ctgatgcggc
     9601 gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt gcccagcgca
     9661 tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt gtaaacgaag
     9721 cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc tggcgcagat
     9781 aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc gtcacctgta
     9841 ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc agcttgagat
     9901 cttcctcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg gcgtaatcca
     9961 gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca tcacgcgtta
    10021 gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag tactgcttca
    10081 actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa acgcccatcc
    10141 ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc agcacgcctt
    10201 cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata ccgataaact
    10261 gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt ttttgctgcg
    10321 gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc ggcagatgcg
    10381 cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc agggtaaagg
    10441 cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg gtggaggtaa
    10501 aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc tgctcgcggt
    10561 taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg cgattaaagc
    10621 cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg acttcgatcg
    10681 aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc agacggctga
    10741 tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg ctgacgtgac
    10801 gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc agatactgat
    10861 cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa caaagcttat
    10921 ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc gccacggctt
    10981 gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg tacgaggtgt
    11041 atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg atttgctgta
    11101 cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg gcggatgccc
    11161 gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc gctgtagttt
    11221 aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa cgggaaggcc
    11281 cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt gcttctcgtc
    11341 ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca actcatcaat
    11401 acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt cgcctgcttc
    11461 cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaattcca actccgcctg
    11521 cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt tttcagtcac
    11581 tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt tctggccttt
    11641 gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac cacgacgttt
    11701 tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt ttcgacgcgc
    11761 ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg ttcgtctttt
    11821 agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc aaagaaaaaa
    11881 ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg gtcttacaat
    11941 ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg tgttgattcc
    12001 atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg tcttcgcctc
    12061 ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg ctctatgggt
    12121 gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt gcacattttc
    12181 agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg catcgcagaa
    12241 ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt atggctttta
    12301 cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata atcgccacaa
    12361 agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag acgcattttg
    12421 tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa gtggcttttg
    12481 caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac cagaaaagcc
    12541 tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt gaagtggctg
    12601 acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg gaagagatga
    12661 agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc ctgcaaggtc
    12721 tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag atgattgagt
    12781 gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac aaactggcaa
    12841 gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg tttaacggtg
    12901 atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag ctgaggcaga
    12961 aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac ggcgaataat
    13021 tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt caatagatta
    13081 caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct tttctttcgc
    13141 ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca gccaaatccg
    13201 attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc tacgcgacta
    13261 actttactct tttttgttca gttcagaaag cactttttgt aatttttttt cagttgttgc
    13321 ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac ttacgaaaaa
    13381 agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact ccccacttcc
    13441 accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat ctgcttcgcg
    13501 acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac ctgcatgatc
    13561 atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat cgcccgtttg
    13621 ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc agccgctgca
    13681 cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc gtccagcgtt
    13741 tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc cagcacgcca
    13801 gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg aatgttcaat
    13861 tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat cgctttcgcg
    13921 ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc tgccgccgtt
    13981 gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc acgcgagaga
    14041 tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac ataatcctct
    14101 ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg aatgttttgc
    14161 aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg ataagaggta
    14221 tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta agtcgacttc
    14281 agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc cagcgccagt
    14341 tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt cagcggttta
    14401 ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag gttaaattct
    14461 tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt gaactcttca
    14521 gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat gcgtgaaagc
    14581 accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt gtgtttctgc
    14641 agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc ggcggcgtaa
    14701 cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc tttacccgca
    14761 atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct gtccatatcg
    14821 tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa cgcaatccca
    14881 cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt ttgcccgacc
    14941 ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg ctcgtgagag
    15001 atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc tacgcctggc
    15061 tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct gtcggtttcg
    15121 gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt cagtgtttct
    15181 tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac ttctggcgct
    15241 tcgtctgcaa cactggtttc ctgcggcttc gcggctgttt ttgccccttt ggcctgtaac
    15301 catttgcccg cttcgacatc agcagtccag cgtttgaact catacttttt gaacagcccc
    15361 aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag ctccagttca
    15421 acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt gttttgctcg
    15481 agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt ttctggctcg
    15541 gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt tttttcaccg
    15601 acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag gaagtcgatg
    15661 atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag gatggtattc
    15721 gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc tttatcgcca
    15781 gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt accgataacg
    15841 tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt aaccatcgcg
    15901 tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg tgatttgtaa
    15961 tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac cgctgcatgc
    16021 gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata catcgcaccg
    16081 gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg ataaagataa
    16141 gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat gtccgtgcct
    16201 gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag tttttcaccg
    16261 cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca tgtggataac
    16321 tttgtgcata aattatttac atcgctttta atttattatc atgcttttta cagcatgatg
    16381 tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg agataatggg
    16441 attttttcta atgtggatag atatgaatta tttttctcct taaggatcat ccgttatttg
    16501 ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct atggctaacc
    16561 ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg ctgagtatcg
    16621 tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc gggattgtaa
    16681 aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt gattttatga
    16741 tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac ctgcaatggg
    16801 aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt tgtaaccacc
    16861 gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt ccgatgaata
    16921 aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg tgctgggcgc
    16981 tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat ccggagcgac
    17041 gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg catcccacca
    17101 ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag caaacccact
    17161 ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg ctaaatgtac
    17221 tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac aataaccgtc
    17281 agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat gtggatttac
    17341 agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt aagcgccatt
    17401 ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg tctctcatgt
    17461 catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag gcgagtgagt
    17521 attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca tctcctgtcc
    17581 gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat tgttgtcaat
    17641 cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt tatcagcctg
    17701 cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg cggtttctgt
    17761 gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt tgtttgtgga
    17821 tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta gttcgccgag
    17881 tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta ataacggtaa
    17941 atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt ttgctaactc
    18001 agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa attggcgcac
    18061 tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga gttgctgatt
    18121 cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat ctttgtcttt
    18181 aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt cttccccgtg
    18241 attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa atttcttttc
    18301 ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt tcattggtga
    18361 taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca atgaactacc
    18421 atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact ctgcaggggc
    18481 aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg cttccacggg
    18541 cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca ctaagttcac
    18601 ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga tgggcaacgg
    18661 accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat tgagatcggg
    18721 tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc ctttgtcaaa
    18781 gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg catcgttaaa
    18841 atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc atgtgccacc
    18901 accccagatt acggctaacg caacaataac acctgtagct gacttacgta tcatataaat
    18961 attccctgat aaaacagcag aactttgaat atccacttat gctgaaataa tgtccagcgg
    19021 caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc ccgtgaatat
    19081 tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat ttcaccgtat
    19141 cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc ggattcagct
    19201 gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca gctgcggctt
    19261 tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg tcgtactctt
    19321 cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa cgaatggtct
    19381 gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg cccagcgcca
    19441 tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg aagttgacgt
    19501 ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca gaaatatgca
    19561 gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc agcacttgcg
    19621 gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca tactgcgccg
    19681 ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt ttcatgatct
    19741 actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag aacttttgcc
    19801 tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa ccacgggaaa
    19861 tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata ataaaccaaa
    19921 cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc gctaaattct
    19981 tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc gccattgagc
    20041 aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc aacaaacatt
    20101 ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg cagaacggtg
    20161 aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc tttcagaaat
    20221 gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg aggctcaatg
    20281 agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg ccccgtctgg
    20341 tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc agcttcgaac
    20401 tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt caataaagtc
    20461 tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag ctgcaacgca
    20521 aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata aaatttgacg
    20581 acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata gctgttaagc
    20641 ggggtaagac cggaatccac ccggatccct tgctcaaaca gagcgtccat gatggtatcc
    20701 gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg aaaacattac
    20761 gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa gcttttactc
    20821 tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat aatctttctg
    20881 caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat agatcagaat
    20941 gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa
    21001 aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga gttcaataac
    21061 ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag acacgttagc
    21121 agggtcaatc ccacaataaa agaggcgata tcggtgaatc tgatgacgac gataacaggc
    21181 gttgtgctgg caggtggtaa agccagacga atgggcggcg tagataaagg attgctggac
    21241 ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca gctctctcac
    21301 gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct gaaagtgatt
    21361 gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt aatgcagcag
    21421 gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc ccatgattta
    21481 gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa
    21541 cgcgatcacc cgactattgc tctggtaaac cacgctattg agcctttatt actggaatat
    21601 ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg tcatgcggtt
    21661 gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga gcttgcccga
    21721 tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt accggaaaaa
    21781 ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt ccggggctga
    21841 ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc tatgagctgc
    21901 gcaaggctgg cgcggcacaa accatcgttg ccagccagca acgatgggcc ttgatgacgg
    21961 aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg gatacctcaa
    22021 agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag attgtgctgt
    22081 ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat gttattgctg
    22141 tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat gatgttgagg
    22201 ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca agagtaggct
    22261 ggatcagacg c
//
LOCUS       DAJTRB010000066        22235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTRB010000066 DAJTRB010000000
VERSION     DAJTRB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.066"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(53..442)
                     /gene="ipgA"
                     /locus_tag="OO330_002696"
     CDS             complement(53..442)
                     /gene="ipgA"
                     /locus_tag="OO330_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5883505.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(455..1939)
                     /gene="icsB"
                     /locus_tag="OO330_002697"
     CDS             complement(455..1939)
                     /gene="icsB"
                     /locus_tag="OO330_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5883506.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            2253..3869
                     /locus_tag="OO330_002698"
     CDS             2253..3869
                     /locus_tag="OO330_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5883507.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            3879..4241
                     /gene="ipgE"
                     /locus_tag="OO330_002699"
     CDS             3879..4241
                     /gene="ipgE"
                     /locus_tag="OO330_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5883508.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            4241..4699
                     /gene="ipgF"
                     /locus_tag="OO330_002700"
     CDS             4241..4699
                     /gene="ipgF"
                     /locus_tag="OO330_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5883509.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            4708..5823
                     /gene="mxiG"
                     /locus_tag="OO330_002701"
     CDS             4708..5823
                     /gene="mxiG"
                     /locus_tag="OO330_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5883510.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            5831..6082
                     /gene="mxiH"
                     /locus_tag="OO330_002702"
     CDS             5831..6082
                     /gene="mxiH"
                     /locus_tag="OO330_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5883511.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            6095..6388
                     /gene="mxiI"
                     /locus_tag="OO330_002703"
     CDS             6095..6388
                     /gene="mxiI"
                     /locus_tag="OO330_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5883512.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            6394..7119
                     /gene="mxiJ"
                     /locus_tag="OO330_002704"
     CDS             6394..7119
                     /gene="mxiJ"
                     /locus_tag="OO330_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5883513.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            7116..7643
                     /locus_tag="OO330_002705"
     CDS             7116..7643
                     /locus_tag="OO330_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5883514.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            7585..8280
                     /gene="mxiN"
                     /locus_tag="OO330_002706"
     CDS             7585..8280
                     /gene="mxiN"
                     /locus_tag="OO330_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5883515.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            8273..8680
                     /gene="mxiM"
                     /locus_tag="OO330_002707"
     CDS             8273..8680
                     /gene="mxiM"
                     /locus_tag="OO330_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5883516.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            8664..9092
                     /gene="mxiM"
                     /locus_tag="OO330_002708"
     CDS             8664..9092
                     /gene="mxiM"
                     /locus_tag="OO330_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5883517.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            9222..9854
                     /gene="mxiE"
                     /locus_tag="OO330_002709"
     CDS             9222..9854
                     /gene="mxiE"
                     /locus_tag="OO330_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5883518.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            9841..11541
                     /gene="mxiD"
                     /locus_tag="OO330_002710"
     CDS             9841..11541
                     /gene="mxiD"
                     /locus_tag="OO330_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5883519.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            11560..12627
                     /gene="mxiC"
                     /locus_tag="OO330_002711"
     CDS             11560..12627
                     /gene="mxiC"
                     /locus_tag="OO330_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5883520.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            12640..14700
                     /gene="mxiA"
                     /locus_tag="OO330_002712"
                     /gene_synonym="virH"
     CDS             12640..14700
                     /gene="mxiA"
                     /locus_tag="OO330_002712"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5883521.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            14713..15114
                     /gene="spa15"
                     /locus_tag="OO330_002713"
                     /gene_synonym="spaK"
     CDS             14713..15114
                     /gene="spa15"
                     /locus_tag="OO330_002713"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5883522.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            15118..16410
                     /gene="spaL"
                     /locus_tag="OO330_002714"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             15118..16410
                     /gene="spaL"
                     /locus_tag="OO330_002714"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5883523.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            16503..16859
                     /gene="spa13"
                     /locus_tag="OO330_002715"
                     /gene_synonym="spaM"
     CDS             16503..16859
                     /gene="spa13"
                     /locus_tag="OO330_002715"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5883524.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            16828..17706
                     /gene="spa32"
                     /locus_tag="OO330_002716"
     CDS             16828..17706
                     /gene="spa32"
                     /locus_tag="OO330_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5883525.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFLYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNIFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            17700..18581
                     /gene="spa33"
                     /locus_tag="OO330_002717"
                     /gene_synonym="spaO"
     CDS             17700..18581
                     /gene="spa33"
                     /locus_tag="OO330_002717"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5883526.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            18586..19236
                     /gene="spa24"
                     /locus_tag="OO330_002718"
                     /gene_synonym="spaP"
     CDS             18586..19236
                     /gene="spa24"
                     /locus_tag="OO330_002718"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5883527.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            19251..19511
                     /gene="spaQ"
                     /locus_tag="OO330_002719"
     CDS             19251..19511
                     /gene="spaQ"
                     /locus_tag="OO330_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5883528.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            19511..20281
                     /gene="spa29"
                     /locus_tag="OO330_002720"
                     /gene_synonym="spaR"
     CDS             19511..20281
                     /gene="spa29"
                     /locus_tag="OO330_002720"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5883529.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            20288..21316
                     /gene="spa40"
                     /locus_tag="OO330_002721"
                     /gene_synonym="spaS"
     CDS             20288..21316
                     /gene="spa40"
                     /locus_tag="OO330_002721"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5883530.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            21329..21571
                     /locus_tag="OO330_002722"
     CDS             21329..21571
                     /locus_tag="OO330_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883531.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         7355 a         3064 c         4397 g         7419 t
ORIGIN      
        1 attcaacctg tggaagtatt ttgtttagaa tttgcatgat accccctata tgttagttca
       61 cttctgaagt gatgtttgca atggttagtt taatacaaaa taaaaattca tacaaagatt
      121 caatgagttc atcaggtgaa agatattttt tgtagtgttt cttggtcaaa acacattgat
      181 gagtatcagg gagaagacag atagaagaac cataatctct actcaaagag tagttaagga
      241 tgagtatgtg ttctggatta ataatatttt ctgtaaaaag ttcatgttca ttaagaagtc
      301 cggtcaaaaa gatgtattca tcattatctg ttatacaaac aggtatttgt tcttcgagca
      361 atataaaggc ttgattttta tcgttaaaat caagctttgc acctgttatt tcataaagtt
      421 tatcatatag tttgcgacac atttctatgg ccttctatat attagaatga gagttattca
      481 ataaaagctt attgatctgt aataagtcgt gaatgtaaag tttggttcca agatctggcg
      541 atttaagaga attgtaataa tcaataattt ttattgattt ttcgatgatt tcttcttttt
      601 tatctgttgc ttcggttttt aattcgttta atacacgagt catattgctg ggtgctgatt
      661 ttactaagta tgagtggcaa gcgttgatga ccgcatcaaa gtcttctgaa gatacatttt
      721 cagtaggatg tttgtaaagt gaaattgata aaggcttgaa tgtttttggt gtgtggataa
      781 ggttaatttt attgaaactt ttaagatagc tgcgtcgtaa ttgagtcaat ctggaaagat
      841 caaaatcaga ttcaatatac ttcattattt cgttataaga ctgattgaga ctttcaatgc
      901 gttgcctaac tttatttgca tacgcgtata catcgttagg tgtagcaacc aatttaacgt
      961 cagggagtgg aaagtatatt tcagaatttc ctgctttaag aacattaagt gccataccag
     1021 cacagttttc agatttactg attaatttat aatttgccaa ttgtctgaaa gcatctgctt
     1081 tctttttgca tataaatttt gacatgtttg tttcatcaag gccaaaaagg ttatggctga
     1141 ttttaccatc ctttgtttta ttatcacctg ataatggaat gtatacttta tcagcactga
     1201 ccccccaata accatagggg tctttttttt gattagctct gggaaaaaat atttttttgt
     1261 tgttattttg atcttgtttt gaatttaata agtttctaac ctcttggcct gcatcaagtc
     1321 tttcggctgt ttggtctgaa atcatattta gtttgtcttg cttataggta tcgacactgt
     1381 aacctgagcg tttttcaaaa aggtagttta tttctgtatc ttttgtaaca ttcccgccgg
     1441 gataccagct gagataggta tgagttggtt cctgtagtaa attgtttttt atactgatac
     1501 tagtatgccc taaatgggtt atgcctctat ttccccagca acgaaaaact aaatgtcttt
     1561 caagattttt ggctgtaccg atgccatgaa aacgctctgc ggtattcaac attaatttga
     1621 tgtctctacc tgtgaaagag tgttgaattg agtttttagc tgcttcatag gcggcaaacg
     1681 cactttctgc agatttttcg ctagctaatg tattgaggaa aagcaataag gatttttgat
     1741 tctctagtcg ataatttttt agttgttcat ttaatatctg tgaatttatc tttgcattaa
     1801 tagtgcttaa tgttctgaaa aagagatcct ttaggttgaa tttctgtgct atcaatatag
     1861 gtccatgctc tgtatcttca acgcgtatag gcccttttgt attgcttgcg tcaatgaaat
     1921 tgctaatttt gaggatcata ctttattaac tctccattac ttggtgattg tggggatgta
     1981 aaatgtattt ggtatataat atattcaatt aataaattta gaaacttgag cctgttaaca
     2041 taatcaaatt ttcttttgct gtacataata tgtacctcgt gagcatatgt agtgctcgtt
     2101 tcatcatgaa atcccacaag ataaagtgcc tgatgtatca ggctcggagt gttatagaaa
     2161 aagagagaac cctgttgaat aagtatcttt tttgttgtaa aacgaggtac aaaactgcaa
     2221 tcaatgcccc atttatgact gaggataatt aaatgcacat aactaatttg ggattgcatc
     2281 aggtttcatt tcaaagcgga gattcctata aaggcgcaga agaaaccgga aagcacaaag
     2341 gtgtaagcgt gatttcatat caaagagtta aaaatggaga aagaaataag gggatcgaag
     2401 cgcttaatcg attatattta cagaaccaaa caagcttaac aggaaaaagc cttttgtttg
     2461 cgcgtgacag agctgaagtt ttttgcgagg caataaagtt ggcaggtggt gatacgtcaa
     2521 aaattaaagc catgatggaa cgattagata cctataaact tggtgaagtt aataaaagac
     2581 atattaatga gcttaataaa gtaataagtg aagagataag agcacagcta ggcattaaaa
     2641 ataaaaagga attgcaaacg aaaataaaac aaatatttac tgattattta aataataaga
     2701 attggggacc ggtcgacaag aatattagtc atcatgggaa aaattatggt tttcaattaa
     2761 ctcctgcctc tcatatgaag atagggaata aaaatatatt tgtcaaagag tataatggaa
     2821 aaggaatttg ttgtgcttct acgagagaga gtgatcatat cgcgaatatg tggctttcaa
     2881 aagtggtgga tgacgagggg aaggaaattt tttcgggtat cagacatgga gttatatcag
     2941 cctatggatt gaaaaaaaac tcatctgaaa gagctgttgc tgctcgtaat aaagctgagg
     3001 agttagtaag cgcagcatta tatagcagac cagagttatt atcacaggct ttgtctggta
     3061 aaacagtaga tttaaagatt gtttcgactt ctcttctgac gccgaccagt ttaaccgggg
     3121 gagaggagag tatgttaaaa gatcaggtga acgcattaaa agggttaaac tctaaaaggg
     3181 ggaagccaac gaaattatta attcggaata gtgatggtct tctgaaagaa gtaagcgtta
     3241 atctaaaagt ggtgacattt aactttggtg taaatgaatt agcgctaaaa atgggcttag
     3301 gctggaggaa tgttgacaag cttaatgatg aatcaatatg ttctttgttg ggggataatt
     3361 tcctcaaaaa tggtgtgatt ggaggttggg ctgcagaagc aatagaaaaa aatccaccat
     3421 gtaagaatga tgtgatatat ctggctaacc agataaaaga gattgtaacc aaaaagttac
     3481 aaaaaaatga taatggagag ccgtataaat tgtcacaaag aatgactcta ttggcttata
     3541 ctataggtgc tgtaccttgc tggaattgta agagtgggaa ggacagaaca ggtatgcagg
     3601 atgctgaaat taaaagagaa ataataagaa aacatgaaac aggtcagttt tctcaattaa
     3661 atagtaaatt atcctcagaa gagaaaagat tattttctac tattctaatg aatagtggta
     3721 atatggaaat ccaagagatg aatactggtg tgcccggaaa taaagtcatg aaaaaattgc
     3781 cgctatcttc gttagagcta tcttattctg aaagaatagg ggacccaaaa atatggaata
     3841 tggtgaaagg gtattcgtca tttgtataag aggaatatat ggaagattta gcagatgtta
     3901 tttgccgagc cttgggtatc cctttaattg atatcgatga tcaagcaatc atgcttgatg
     3961 atgatgtgct aatatatatt gaaaaagaag gagattcaat aaatttatta tgcccttttt
     4021 gtgcattgcc tgaaaatata aatgatctta tatatgcgtt gagcctaaat tactcagaaa
     4081 agatatgctt agctactgat gatgaaggcg gaaatctaat tgcgcgatta gatcttaccg
     4141 ggataaatga attcgaggat gtatatgtta atacagaata ttatatttcg cgtgtgcgtt
     4201 ggctgaagga tgaatttgcg cgaagaatga aggggtatta atgtcccgtt tcgtatttat
     4261 tttgctgtgt tttatcccat atttagggag agctgattgt tgggataagg ctggtgaaag
     4321 gtacaatatt ccatcaagtt tgttaaaagc gattgcggaa aaagaatccg gatttaataa
     4381 atctgcagtc aatgttaata acaatggaag taaagattat ggtataatgc aaataaatga
     4441 ctttcattct aaaagactta gagaaatggg atattctgag gaaatgctaa ttagtcatcc
     4501 atgtctttct gtacattatg cagcaaagtt attaaatgag tttatgatga tgtatggaag
     4561 agggtgggag gctgttggag cctataatgc aggaacgtct ccgaaaaaga aaaaagaaag
     4621 attaaagtac gcagaggata tctatagacg ctatttgcgg atagcagcag agagcaagca
     4681 gaataatcga aggatataag aggatttatg tctgaggcaa agaactcaaa tcttgcacca
     4741 ttcaggttat tagtgaaact cacaaacggt gtcggggatg agtttccgtt atattatggg
     4801 aataacttaa ttgttctcgg acggacgata gaaacacttg agtttggaaa cgacaatttc
     4861 ccagaaaata ttattcctgt aacagatagt aaatcggatg gtataattta tttgaccata
     4921 agcaaagata atatctgtca atttagtgat gaaaaaggtg agcaaattga tatcaattca
     4981 caatttaatt catttgaata tgacgggatt tcttttcacc ttaaaaatat gagggaggat
     5041 aaatcgagag gccatatact aaacggaatg tataaaaacc actcggtgtt ttttttcttt
     5101 gctgttattg ttgtgttaat tataattttt tccctttctt taaagaaaga tgaagtaaaa
     5161 gaaattgctg aaattataga tgataagaga tatggaatag tcaacactgg acagtgcaat
     5221 tatattcttg ccgaaacaca aaatgatgcg gtatgggcgt ctgtagcctt aaataaaacc
     5281 ggatttacca aatgcagata tattctggtg tcaaacaaag aaataaatag aattcaacaa
     5341 tatatcaatc aacgattccc atttatcaat ttgtatgtgt tgaatttagt gagtgataaa
     5401 gcggaattac tggtgttttt aagtaaagaa agaaatagtt caaaagatac cgaacttgat
     5461 aaattgaaaa atgctctgat tgttgaattt ccgtatatca aaaatattaa attcaactat
     5521 cttagcgatc ataatgcaag gggggatgct aaaggtattt tcacaaaggt aaatgtccag
     5581 tataaagaga tttgtgaaaa taataaagtt acatattctg tcagagaaga actgacagat
     5641 gaaaagcttg agctcattaa tagactgatt tctgaacata aaaatatata tggagatcag
     5701 tatattgagt tttctgtact tttgatagat gatgatttta aaggtaaatc atatcttaac
     5761 agcaaagaca gttatgtaat gttgaatgat aaacactggt tttttttaga taaaaataag
     5821 tgaggataaa atgagtgtta cagtaccgaa tgatgattgg acattgagtt cattatctga
     5881 aacttttgat gatggaactc aaacattaca aggtgaacta acattggcac tagataaatt
     5941 agctaaaaat ccttcgaatc cacagttgct ggctgaatac caaagtaaat tatctgaata
     6001 tacattatat aggaacgcgc aatccaatac agtgaaagtg attaaggatg ttgatgctgc
     6061 aattattcaa aacttcagat aatagggagc attcatgaat tacatttatc cagtcaatca
     6121 ggttgatatt atcaaagcca gtgattttca atctcaagag atatcaagtc tggaagacgt
     6181 cgtgtcggct aaatatagtg atattaagat ggatacagat attcaagtat cacaaataat
     6241 ggagatggta agcaatccag aatcattaaa cccagaatct ttggccaagt tacagacgac
     6301 gctctcaaat tattcaatag gagtatcatt agctggcacg ttagcaagaa aaacagtttc
     6361 ggctgttgaa actttattaa agtcttaatt tatatgatta ggtataaagg ttttatttta
     6421 ttcttgttgc tgatgttgat tggatgtgag caacgtgaag agttaatttc taatttatct
     6481 caaagacagg caaatgaaat aatatctgtg ctagaacgcc ataatattac tgctagaaaa
     6541 gttgatggag gtaaacaggg gatctcggta caagtcgaaa aggggacatt tgcatcggca
     6601 gttgatttga tgcgcatgta cgatttgcca aatccggaga gagttgatat ctcacaaatg
     6661 tttcctacag attcattagt gtcttctcca agagctgaaa aggcccgttt atatagtgct
     6721 attgagcaac ggctggaaca gtctttagtt tctattggtg gtgttatttc ggcaaaaata
     6781 catgttagct atgatcttga agaaaaaaat atatcttcaa aaccgatgca tatatcagta
     6841 atcgctatat atgactcacc gaaagagtct gaactattag ttagtaatat taagcgattt
     6901 ttgaaaaaca ccttttctga tgttaagtat gaaaatatat ctgtcatatt aactccgaaa
     6961 gaagaatatg tttatacaaa tgtacaacct gttaaggaag ttaaatcgga atttttaaca
     7021 aatgaagtaa tatatttatt tctcgggatg gctgtactag ttgtcattct tttggtatgg
     7081 gcattcaaaa cagggtggtt caagagaaac aaaatatgat aagaatggat ggaatttata
     7141 aaaaatatct ttcaataatt tttgatccag cgttctatat aaatagaaat cggttgaatt
     7201 tgccttctga actgttagaa aatggcgtaa tcagaagtga gattaataat ctcataatta
     7261 ataaatatga tctaaattgc gatattgaac ctttaagcgg ggtaaccgct atgtttgttg
     7321 ccaactggaa tttacttcca gctgttgctt attttatcgg tagtcaagag tctcggttaa
     7381 ttaatcatag tgagatggtt attagttatt atgggggcaa aatcagtaag caaggggagg
     7441 ccgctattcg aagtggtttt tggcatctta ttgcgtggaa ggaaaatata tctgttggta
     7501 tatatgaaag aataaactta ttatttaatc caattgcttt ggagggaaat tatacgcctg
     7561 tagaaagaaa tttgtcccga ttaaatgaag gtatgcaata tgcaaaaagg cactttaccg
     7621 gtatccagac atcatgccta tgatggtgtt gttataaaga gaattgaaaa ggaattatgt
     7681 aaaaccataa aagatagaga cactgagagt aagaagaaag caatttgtgt tataaaggag
     7741 gctacgaaaa aagctgaatc actgcgtata gatgctgtat gtgatggtta tcaaattggt
     7801 atacagactg cttttgagca tattattgac tatatttgtg aatggaagtt aaagcaaaat
     7861 gaaaacagaa gaaatattga ggactatata actagtttgc tttcagaaaa tttacatgat
     7921 gagaggatca ttagcacttt attggagcag tggcttagtt cgttgcgtaa tacagtaaca
     7981 gaattaaaag tggttttgcc caagtgtaat ttagcactga ggaaaaagtt agaacttgat
     8041 ctccataaat atcgctcaga tgtcaagatt attttaaaat attctgaagg gaataattat
     8101 atattctgta gtggtaatca agtagtggag tttagtcctc aggatgtaat atcaggtgtt
     8161 aaaatagaac ttgcggaaaa attaacaaaa aatgataaaa aatatttcaa agagttagca
     8221 cataagaaac tgagacaaat tgcagaagac ttgttaaaag agaatcctgt taatgattaa
     8281 tcaaataaat gcaagcaatg ctctgcagca aagattaaat agtgaagaat ttgttaattt
     8341 aaatgagaga ttatcatctt cgcaatcatt tgatgaggat ataatctatg aaataatgca
     8401 atatttctca cagagtgaat taaattccat agataatgat gaactgcaca ataagattga
     8461 acaattattc aattcacgtt ttccttatct tacagctgca caaaaaagtt cacttcttaa
     8521 taaactcata gatgcaaatc aatatgtcga tttgcatgag ggattttatg catcattgtc
     8581 catatataac aatattgatt tctatattaa gactacaacc ttcgattcat taattagtgt
     8641 ctttgaagca gggagagagg cagatgattc gacatggtag taataagttg aaaatattta
     8701 ttttaagtat attgctatta acactgagtg ggtgtgcttt aaagtcatca tctaattctg
     8761 aaaaagaatg gcatattgtt cctgtaagta aggattattt ttctattcca aatgatttat
     8821 tatggtcgtt taatacaacc aataaaagta taaatgttta ctctaaatgt attagtggta
     8881 aggcggttta tagttttaat gcaggtaaat tcatgggcaa ctttaatgtt aaggaagtag
     8941 atgggtgctt catggatgca caaaagatag ctatagataa actattttct atgctgaaag
     9001 acggggttgt tttaaaaggt aataagataa atgataccat ccttatagag aaggatgggg
     9061 aagttaaatt aaaattaatt cgagggatat aattgtattg tgagtaaata taaaggtcta
     9121 aatacaagta atatgtttta catttactct agtggacatg aaccagttaa cgttgagctt
     9181 gtaaaagata aagaacgtaa cataattgag ctggctccag catggaaggg tttttttttg
     9241 tccgaaatca aaatataaaa ttcagtgata atgtgaacta tcattatcgc tttaacatta
     9301 atagttgtgc aaaatttctt gctttttggg actatttcag tggtgctcta gtagagcata
     9361 gccatgctga aaaatgtatt catttctatc atgagaatga tcttcgggat agttgtaata
     9421 ccgaatcgat gcttgataag ttaatgttaa gattcatctt ttcatctgac cagaatgtaa
     9481 gtaatgcact ggctatgata cgaatgactg agtcatatca tttggttctt tatcttctga
     9541 gaactattga gaaagaaaag gaggtaagaa taaagagtct gacagagcat tatggtgtgt
     9601 cggaggctta ttttagatct ttatgtagaa aagctttagg ggcaaaagtc aaagaacaac
     9661 tgaacacttg gcggttagta aatggtttat tagatgtatt tttgcacaat caaacaatca
     9721 cttcagctgc catgaataat gggtatgcat caacatccca tttttcaaat gagataaaaa
     9781 cacgactagg cttttctgca agagaattat cgaatattac ttttttagtg aaaaaaataa
     9841 atgaaaaaat ttaatattaa atctttgact ctcttgattg tattgttacc attgatagtt
     9901 aatgctaata atattgactc tcatttatta gaacaaaatg acatagcgaa atatgttgct
     9961 caaagtgata cggtaggatc tttttttgaa cgtttttctg cgttattaaa ttatcctatt
    10021 gttgttagca aacaagctgc aaaaaagaga atatctgggg agtttgattt atctaatcca
    10081 gaagaaatgc ttgaaaaact gacattactt gtgggactca tatggtataa agatggaaat
    10141 gcattataca tatatgactc tggagaatta ataagcaaag ttattttgct ggagaatata
    10201 tcacttaatt atcttattca gtatctgaag gatgctaacc tttatgatca cagatatcct
    10261 ataagaggaa acattagtga taaaacattc tatatatctg gtcctccggc attggttgaa
    10321 ctggtagcta atacagcaac tttgcttgat aagcaggttt cttctattgg gacagataaa
    10381 gttaattttg gggttataaa attaaaaaat acatttgtta gtgacagaac ttataatatg
    10441 agaggggagg atattgtcat cccaggggtg gctactgttg tagaacgttt attaaataat
    10501 ggtaaggctc tgagtaacag acaagcccaa aatgatccga tgccaccctt taatattaca
    10561 cagaaagtat ctgaggatag taatgacttt tcatttagtt cagttaccaa tagttctata
    10621 ttagaggatg tatcacttat tgcatatcca gaaaccaatt ctatattagt caaaggtaat
    10681 gatcagcaaa tacagattat tcgcgatatt atcactcagt tagatatagc caaacgacat
    10741 atagaattat cattatggat aatcgatatt gataaatctg aattaaataa ccttggtgtt
    10801 aattggcaag gaacggcttc atttggtgat tcgtttggtg cgtcatttaa tatgagctca
    10861 tcagcatcga taagcacttt ggacggcaat aagtttattg cttcagttat ggcattaaat
    10921 cagaaaaaga aagcaaatgt agtttcaagg ccggtgattt tgacacaaga gaatattccg
    10981 gctatatttg ataataacag gactttttat gtgtcattag ttggtgagcg caattcttcg
    11041 ttggagcatg ttacgtatgg aacattgata aatgttatac ctcgcttttc atctcgggga
    11101 caaattgaga tgtctttaac gatagaggat ggaacaggaa atagtcagtc aaattacaat
    11161 tataataatg aaaatacatc tgtccttcca gaagttggaa ggaccaaaat cagtacaata
    11221 gcgagagttc cacaaggcaa aagtttgttg ataggtggtt atacgcatga aacgaactct
    11281 aacgaaataa tatctattcc ttttctctcc tctattcctg taatcggtaa tgtgttcaag
    11341 tataaaacaa gtaatatcag taatattgta agggtttttc tgattcaacc ccgtgagata
    11401 aaggagtcaa gttattataa tactgcagaa tataaaagcc tcatatctga aagagaaata
    11461 caaaagacta cacaaataat cccatctgaa actactttgc tggaagacga aaaatcattg
    11521 gtttcatact taaattacta actataaagt aggtgatgta tgcttgatgt taaaaataca
    11581 ggagttttta gctctgcatt cattgataag ttgaatgcaa tgacaaattc agatgatgga
    11641 gatgagactg ctgatgcaga gcttgattct ggcttggcta atagcaagta tattgactca
    11701 tctgatgaga tggcttccgc tctttcgtca tttataaaca gaagagacct tgagaaactg
    11761 aaaggaacaa atagtgatag tcaggaacgt attttagatg gggaagaaga tgaaattaat
    11821 cacaagattt ttgatttaaa gagaacgtta aaagataacc ttcctctaga tcgggatttt
    11881 atagacagac taaagagata ttttaaagat ccaagtgatc aagtcttagc attaagggaa
    11941 cttttgaatg aaaaagatct tactgctgaa caagtcgaat tattaactaa aattattaat
    12001 gagataatat caggtagtga aaaaagtgtt aatgctggaa taaattcagc tatacaggct
    12061 aaattatttg gcaacaaaat gaaacttgaa ccacagcttt tgcgtgcatg ttatcgtggt
    12121 tttatcatgg ggaacatatc aacaacagat cagtatatag aatggcttgg taattttggt
    12181 tttaatcaca gacatacaat tgtgaatttt gtagagcagt cactgattgt agacatggat
    12241 tctgagaaac cgagctgtaa tgcttatgag tttggttttg tgttatctaa attaattgca
    12301 attaagatga ttagaacttc agacgtaatt tttatgaaga aactggaatc ctcaagcttg
    12361 ctaaaagatg gcagtttaag tgcagagcag ctattgctaa ctttattata tatttttcaa
    12421 tatccaagtg aaagtgagca aattcttact tctgttatag aagtatcacg agccagtcat
    12481 gaggattctg tagtgtatca aacatatcta tcttctgtta atgaaagtcc tcatgatata
    12541 tttaaaagtg aaagtgaaag agaaattgcg atcaatattc tacgagagct tgtcacaagt
    12601 gcatacaaga aagagctttc tagataacag gagataaaag tgatccagtc ttttcttaag
    12661 caagtaagta ctaagcctga attaattata ttagtgctta tggttatgat cattgctatg
    12721 ttgatcatac cattacctac ataccttgtt gattttttaa tagggcttaa tattgtactt
    12781 gctattctcg tttttatggg atcattttat attgaaagaa tattgagttt ttctacattc
    12841 ccttcggttt tgttgataac gacattattt cgtctcgctt tatcaataag tactagtcga
    12901 ctaattttag ttgatgcgga tgcgggtaaa attattacta catttggcca gtttgtcatt
    12961 ggtgatagtc tggctgtagg ttttgttatt ttttcaatag taactgttgt gcagttcatt
    13021 gttataacaa aaggatctga aagggttgcg gaggtagctg ctcgcttttc tcttgacggt
    13081 atgccgggga aacaaatgag cattgatgcg gatttaaagg ctggaattat tgatgccgca
    13141 ggagctaaag aaagacgcag tattctggaa cgtgagagtc aattgtatgg ttcatttgat
    13201 ggagcaatga agttcattaa aggtgatgca attgctggca tcattattat ttttgtcaat
    13261 ttaataggag gtatttctgt tggtatgagt caacatggaa tgtccctatc tggtgcatta
    13321 tctacttata ccatactaac tattggtgac ggattggtct ctcagattcc tgctctgtta
    13381 atttctataa gtgcaggatt tatcgttaca agagttaatg gtgatagcga taatatggga
    13441 cgtaacataa tgtcccagat atttgggaat ccttttgttc tcattgttac atcagctctt
    13501 gccttggcga tagggatgtt gccaggtttt ccgttttttg tttttttcct gatagcagtt
    13561 actttgacgg ctttattcta ttataaaaag gtcgtagaaa aagaaaaaag tctgtctgag
    13621 tctgattcta gtggctatac tggtacattt gatattgata atagccatga ctcatctttg
    13681 gcaatgatag aaaatctgga tgctattagt tcagaaaccg ttcctttaat tttattattt
    13741 gccgaaaata agataaatgc aaatgatatg gaaggtctta ttgaaaggat aagaagtcag
    13801 ttctttattg attatggtgt caggcttcca actattttat atagaacaag caatgagctt
    13861 aaggtcgatg atattgtttt gttaataaat gaggtgcgtg ccgatagttt taatatatat
    13921 tttgacaaag tgtgtattac agatgaaaat ggagatatag atgctctagg tattcctgtt
    13981 gtatcaactt catataatga acgtgttatt tcttgggtag acgtttcata tacagaaaat
    14041 ctaactaata ttgatgctaa aattaaaagt gctcaagatg aattttatca ccagttgtca
    14101 caagctttat taaacaacat aaatgagatt tttggtatac aagaaacaaa aaatatgtta
    14161 gatcagtttg aaaatcggta tcctgatcta ttaaaggaag tcttccgaca tgtgactata
    14221 cagagaattt ctgaggtatt acaaagattg cttggagaaa atatttctgt tcgcaattta
    14281 aaacttatta tggagtcttt ggcgctttgg gctcccagag aaaaagatgt cataacatta
    14341 gttgaacatg tccgtgcatc actttctagg tatatttgta gtaaaatagc tgtttctggt
    14401 gagattaaag ttgtgatgct ttccggatat attgaggatg caataagaaa agggataagg
    14461 caaacctctg gtggctcttt cttgaatatg gatatagagg tttcggatga ggtaatggaa
    14521 actttagcac atgctttgag agaattgaga aatgcaaaaa aaaatttcgt tcttttggta
    14581 tcagtagata tacgtaggtt tgttaaaaga cttatagata acagatttaa gagtatactc
    14641 gttatatcgt atgctgagat tgatgaagca tataccatta atgtattaaa gactatttag
    14701 tgaggtttaa atatgagtaa cattaattta gttcaattag ttagagatag tcttttcacg
    14761 attggttgtc ctccatcaat tattacagat ttagacagcc attcagcgat taccatttct
    14821 ttggatagta tgcctgctat taatatagct cttgtcaatg agcaggttat gttatgggct
    14881 aattttgatg caccaagtga tgtaaaactt cagtcatctg cttataacat attaaattta
    14941 atgttaatga actttagtta tagcattaat gagctggtag agcttcatcg ttctgatgag
    15001 tacttacagt taagagttgt tattaaagat gactacgttc acgatgggat tgtttttgca
    15061 gagattctgc atgagtttta tcagaggatg gaaatcttaa atggggtctt ataatcaatg
    15121 agctatacaa aattgctcac tcaattatct tttcctaata gaatctcggg gccaatcttg
    15181 gaaacaagtc ttagcgatgt ttcgattggt gagatttgta acattcaggc tggaattgaa
    15241 agtaatgaaa ttgttgcaag agctcaggtt gtaggatttc atgatgaaaa aacaatatta
    15301 agcttgattg gaaattctcg tggactttca cggcaaacgt tgattaagcc cactgcccag
    15361 tttcttcata cgcaagtcgg ccgtggatta ttgggagcag tagtcaatcc tttaggggag
    15421 gttactgata agtttgctgt tacagataac agtgaaattc tttatcgacc tgtagataat
    15481 gctcctccgc tatatagtga aagggctgca attgagaagc cttttttaac aggtattaag
    15541 gttattgatt ctttactcac gtgtggtgaa ggacagcgaa tggggatttt tgcgtcagct
    15601 ggttgtggca aaacttttct catgaatatg ctcattgaac atagtggtgc tgatatatat
    15661 gttattgggt taattggtga gcgaggtcga gaggttactg aaacggttga ttatttgaaa
    15721 aactctgaga aaaaaagcag gtgtgtttta gtatatgcaa cttcggatta ctcttcggtt
    15781 gatcgttgta atgctgcata tatagccact gctatagccg aattttttag gactgaagga
    15841 cataaagtag cgctttttat tgattcatta acaaggtatg ccagagcatt acgtgatgtg
    15901 gccttagccg ctggagaatc acctgccaga agaggctatc cggtttcggt ttttgatagc
    15961 ttacccagac ttcttgaaag gccaggaaag ttaaaggcag gtggctctat tactgcattt
    16021 tacactgttc ttttggagga tgatgatttt gctgatccat tagctgaaga ggtaagatcc
    16081 attttagatg gacatatata tttgagcaga aatctagccc aaaaaggaca atttcctgca
    16141 attgattcct taaaaagtat aagcagggta tttacacagg ttgttgatga aaaacatcgt
    16201 attatggccg ctgcatttcg ggagctactt tctgaaatag aagagctaag gacaattatt
    16261 gactttggtg aatacaaacc gggggagaat gcctctcagg ataaaatcta caacaaaata
    16321 tctgttgttg agagttttct gaagcaagat tatcgactcg gttttactta tgagcagaca
    16381 atggagctta ttggtgaaac aattagataa ggttttaaaa atcaaagata aatatcaacg
    16441 gtctgttaaa ttaattgaag cacacatatt aacactaaaa aaaaaactct ttgtatcgag
    16501 atgtggaggc attagataaa aggattatat acttcctgca actggagaat gacttagaac
    16561 ctgtaggcgc tcagagtgtt agtcagcttt tcaatacaag acgtaagatc gctatagtaa
    16621 aaaagcatat tatccaatat cagtctgaac gaattcttct aaagggaaga attgaagaaa
    16681 ttcaaaaaga tattgatgag gcaaatgctt ctaaaagaaa actgcttcat aaagagagta
    16741 aaatatgtaa acggattggc ttgattaaaa gaaataactt tgccaaacag ttgatacttg
    16801 atgagttatc acaagaggat atgaagtatg gcattagaca atataaacct aaattttagt
    16861 agtgacaaac agatagaaaa atgtgagaaa ctatcttcaa tagataatat tgatagtctc
    16921 gttttgaaga agaagaggaa ggttgaaatt cctgagtact ctttaattgc atctaactat
    16981 tttactattg ataagcactt tgaacataag catgataaag gagaaattta tagtggcatt
    17041 aaaaatgcgt tcgaacttag aaacgaacga gcgacatatt ctgatattcc agaatcaatg
    17101 gccattaaag aaaatatttt gataccagat caagatatca aagcaaggga aaaaataaat
    17161 atcggcgata tgagggggat ctttttatat aataagagtg gaaatgcaga caagaacttc
    17221 gaaagaagtc atacttcttc tgtaaaccct gataatctgc tagaatctga taatagaaat
    17281 ggtcaaattg gtttaaaaaa tcatagcttg tctattgata agaatattgc tgacatcatt
    17341 tctttactaa atggaagtgt tgctaaatca tttgagctgc ctgtaatgaa taaaaatact
    17401 gcagacataa ccccatccat gtcattgcaa gaaaaatcaa tagttgaaaa tgataaaaat
    17461 atttttcaaa aaaatagtga aatgacttac cactttaaac agtggggggc tggacattct
    17521 gttagtattt cagtggagtc tggttctttt gttctaaaac cgtcagatca atttgtagga
    17581 aataaacttg acttaatttt gaaacaagat gctgagggta attacagatt tgatagcagt
    17641 caacataata aggggaataa aaataatagt acaggatata atgaacagag tgaagaagaa
    17701 tgctaagaat taaacatttt gacgctaacg aaaaactaca gattttatat gcaaagcaac
    17761 tctgcgagcg tttttcgatt cagacattca aaaataaatt tacaggcagt gaaagtttag
    17821 tcactcttac ttccgtgtgt ggggattggg taattcgtat tgatacatta tcttttttga
    17881 aaaaaaaata cgaggtattt tcaggatttt ctacacaaga atctttactg catttatcaa
    17941 aatgtgtctt tatagagtcg tcatctgtat tttcgattcc agaactgtct gataagatta
    18001 ctttccggat cacgaatgaa atccagtatg caactactgg gagtcattta tgctgttttt
    18061 cctcttcttt aggtattatt tattttgaca agatgccggt attacgtaat caagtttctc
    18121 ttgactcatt gcatcatctt ttagagtttt gcttaggttc atctaatgta aggctggcta
    18181 ctttaaaaag aattcgcact ggtgatataa tcatagttca gaaactttat aatttattat
    18241 tgtgtaatca agttattatt ggggattata ttgtgaatga taataatgag gcaaaaatta
    18301 atctgtcaga aagtaatggt gagtcagaac acacagaagt ttctttggca ttattcaatt
    18361 atgatgatat caatgtaaaa gtggacttta ttcttttaga aaaaaatatg acaatcaatg
    18421 aactaaaaat gtatgtagaa aacgaattat tcaagtttcc cgatgacata gttaaacatg
    18481 taaatattaa agtaaatggt tctttggttg ggcatgggga acttgtttct attgaggatg
    18541 gttatggtat cgagattagt tcttggatgg taaaggagta atctcatgct gagtgacatg
    18601 tccctcatcg ctacactttc tttttttacg ctattaccat ttttagttgc tgctggtacc
    18661 tgttatataa agttttctat tgtttttgta atggtacgaa atgcacttgg tttgcagcag
    18721 gttccatcaa atatgactct caatggtatt gcattgatta tggctttgtt cgtgatgaaa
    18781 cccattattg aagctggtta tgaaaattat ctaaatggcc ctcaaaaatt tgatacaata
    18841 tctgatattg tccgtttttc tgattctggc ttgatggaat ataagcaata cttgaaaaag
    18901 catacagatc ttgagttagc acgttttttc caaaggtctg aagaagaaaa tgctgattta
    18961 aagagtgcag agaataatga ttattcattg ttttccctct tgcctgcata tgcgctaagt
    19021 gaaatcaaag atgcgttcaa aataggtttt tatttatatt tgccatttgt tgttgttgat
    19081 cttgttattt caagcatttt acttgcactt ggtatgatga tgatgagtcc aatcacaata
    19141 tcagtaccaa taaaacttgt attatttgtt gcgcttgatg ggtggggtat cctgtctaag
    19201 gccttgattg aacaatatat caatattcct gcttagatga tggaggtaat gtgtctgata
    19261 tagtttatat gggtaataag gctctttatt taatccttat cttttcctta tggccagtag
    19321 gtatagctac ggttattgga ttatctattg gtttgttaca gacagtgact caactccaag
    19381 agcagacact tccttttggt ataaagctta taggtgtctc aatatctttg ctacttcttt
    19441 ctggatggta tggtgaggtt ttattgtctt tttgtcatga aataatgttt ttaattaaga
    19501 gtggggtttg atggacattt caagctggtt cgaaagtatt catgtgtttt taatactcct
    19561 gaacggcgtt ttttttagat tggctccatt atttttcttt cttccatttt taaataacgg
    19621 tataatttct ccatctatta gaatacctgt gatttttctt gttgcatcag gattaattac
    19681 ttctggtaag gtagacatag gttcttctgt ttttgaacat gtttatttcc ttatgttcaa
    19741 ggaaataatt gttggcctcc ttctctcttt ttgcttgtct cttccctttt ggatatttca
    19801 tgctgttggt agcattattg acaaccagcg tggggcaacg cttagtagtt caattgatcc
    19861 tgccaatggt gttgatacgt ctgagttggc aaaatttttc aatctttttt ctgcagttgt
    19921 atttctatac agtggtggta tggtctttat tttagaatcc atacaattgt cttataatat
    19981 atgcccgtta ttttctcaat gttctttccg tgtctcaaat atcttaacat ttctgacttt
    20041 attggcaagt caggctgtta ttttagccag tcctgttatg atagtattgt tactatcaga
    20101 agtattactt ggtgtattat cgagatttgc tccgcagatg aatgcttttt ccgtatcatt
    20161 aactattaaa agtttacttg caatatttat tattttcatc tgttcttcta ctatttactt
    20221 ttctaaagtt caatttttcc tcggtgaaca taaatttttc acaaatctat ttgttagata
    20281 aaatattatg gcaaataaaa cagaaaagcc gacacctaaa aaactaaagg atgccgcaaa
    20341 aaaaggacag tcatttaaat ttaaggattt aacgactgtt gttattattc tggtagggac
    20401 atttactata atatcattct tttccttaag tgatgtaatg ctcttataca gatatgtaat
    20461 aattaatgac ttcgaaatta atgagggtaa atactttttt gcagtggtta ttgtcttttt
    20521 taagataatt ggcttcccac tttttttctg tgttctttcg gctgtgttgc caacattggt
    20581 tcaaacaaag tttgttcttg cgactaaagc tatcaagatt gatttttcag tattaaaccc
    20641 tgttaaaggg ttaaaaaaaa tatttagtat aaagacaata aaagaatttt tcaaaagcat
    20701 tctgcttctt attattctag cattaacaac ctatttcttt tggattaatg accgaaaaat
    20761 aattttttct caggtgtttt ctagtgttga tggcttatat cttatttggg ggaggctgtt
    20821 taaggatata atattatttt tcttggcatt ttctattctt gttattattc ttgactttgt
    20881 gattgagttc attttataca tgaaagatat gatgatggat aaacaggaga taaaaagaga
    20941 atatatagag caagagggac actttgagac aaagtcgaga aggcgtgagt tgcatatcga
    21001 gattctttca gagcagacta aatctgatat acgtaattca aaattagtgg taatgaaccc
    21061 gactcatatt gcaattggta tttattttaa tccagaaata gcgcctgcac cttttatttc
    21121 tctcattgaa actaaccagt gtgccttggc tgtcagaaaa tatgcaaatg aagttggtat
    21181 accgactgtg cgtgatgtga aattagctag aaaactatat aaaacacata caaaatatag
    21241 ttttgttgat tttgaacact tggatgaagt cctacgtctt attgtttggc ttgagcaggt
    21301 tgaaaacact cattaataag taaaggagat gtgttatatg ggagttaatt tctgtaataa
    21361 aataggtatt gatcagtctg aatttgaaat agaatcttcc atcattaact ccattgctaa
    21421 tgaggtattg aacccaatat ctttccttag caataaggat ataataaatg ttttactcag
    21481 aaagatttca tctgaatgtg acctggttag aaaagacatt tatcgttgtg ctctggagtt
    21541 ggtcgttgaa aaaacacctg atgatttata atcattcata ataactcttt tgctgatata
    21601 aatatttttg ttgaagatat ttatgtagtc acaggatcaa ttgtagaaaa ttgttatgtt
    21661 tttgttagga actgcactaa ataatatatg ataagacagc aaaaacgact gaccataatc
    21721 cttcttttat taggagttga taagagagac tattcatcct gtaatgttaa aactctgtta
    21781 tatagtattc gcgattatgc aaagtctgta aatgatcatg agatactaac cgagagtaat
    21841 cgtttattat ctcattgcat ttcagatagt aacggagctt tctttaaaag tagtaaatac
    21901 gttcccctta agtacttacg taaaaggaga atagcacgaa agataccaaa tgattagcac
    21961 tttgctagta agcttgctgg taaactatat tgaccgttgg gtttgttttg agactgtaat
    22021 ttaaattgtg taattgcctg tttttgatat gtttactcca acaacggaga caggtaaatt
    22081 atggacgaaa agaagcttaa agcccttgcg gctgaactgg ctaaaggcct caaaactgag
    22141 gccgacctca atcagttttc ccgtatgctg acgaaatatc ccggctctta aaacgatagt
    22201 ggctcaggct ttcatttttc agcgttcccc agaag
//
LOCUS       DAJTRB010000067        22192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTRB010000067 DAJTRB010000000
VERSION     DAJTRB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.067"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..246)
                     /locus_tag="OO330_002723"
     CDS             complement(<1..246)
                     /locus_tag="OO330_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5883532.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQS"
     gene            254..754
                     /gene="tnpA"
                     /locus_tag="OO330_002724"
     CDS             254..754
                     /gene="tnpA"
                     /locus_tag="OO330_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5883533.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(793..1140)
                     /gene="grxD"
                     /locus_tag="OO330_002725"
     CDS             complement(793..1140)
                     /gene="grxD"
                     /locus_tag="OO330_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5883534.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1475..2302
                     /gene="mepH"
                     /locus_tag="OO330_002726"
     CDS             1475..2302
                     /gene="mepH"
                     /locus_tag="OO330_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5883535.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2430..3011
                     /gene="sodB"
                     /locus_tag="OO330_002727"
     CDS             2430..3011
                     /gene="sodB"
                     /locus_tag="OO330_002727"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5883536.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3157..4326)
                     /locus_tag="OO330_002728"
     CDS             complement(3157..4326)
                     /locus_tag="OO330_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5883537.1"
                     /translation="MKINYPLLALAIGALGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4492..4581)
                     /gene="ynhF"
                     /locus_tag="OO330_002729"
     CDS             complement(4492..4581)
                     /gene="ynhF"
                     /locus_tag="OO330_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5883538.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4880..5905
                     /gene="purR"
                     /locus_tag="OO330_002730"
     CDS             4880..5905
                     /gene="purR"
                     /locus_tag="OO330_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5883539.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5902..6834)
                     /locus_tag="OO330_002731"
     CDS             complement(5902..6834)
                     /locus_tag="OO330_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5883540.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6947..8158
                     /locus_tag="OO330_002732"
     CDS             6947..8158
                     /locus_tag="OO330_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5883541.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8449..9597
                     /gene="cfa"
                     /locus_tag="OO330_002733"
     CDS             8449..9597
                     /gene="cfa"
                     /locus_tag="OO330_002733"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5883542.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9637..10278)
                     /gene="ribE"
                     /locus_tag="OO330_002734"
     CDS             complement(9637..10278)
                     /gene="ribE"
                     /locus_tag="OO330_002734"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5883543.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10493..11866
                     /gene="mdtK"
                     /locus_tag="OO330_002735"
     CDS             10493..11866
                     /gene="mdtK"
                     /locus_tag="OO330_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5883544.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11907..13163)
                     /locus_tag="OO330_002736"
     CDS             complement(11907..13163)
                     /locus_tag="OO330_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5883545.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13471..13547
                     /locus_tag="OO330_002737"
     tRNA            13471..13547
                     /locus_tag="OO330_002737"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13505..13507,aa:Val,seq:gac)
     gene            13552..13628
                     /locus_tag="OO330_002738"
     tRNA            13552..13628
                     /locus_tag="OO330_002738"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13586..13588,aa:Val,seq:gac)
     gene            13736..14041
                     /locus_tag="OO330_002739"
     CDS             13736..14041
                     /locus_tag="OO330_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5883546.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14167..15771
                     /locus_tag="OO330_002740"
     CDS             14167..15771
                     /locus_tag="OO330_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR5883547.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(15783..16595)
                     /locus_tag="OO330_002741"
     CDS             complement(15783..16595)
                     /locus_tag="OO330_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883548.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16599..17384)
                     /locus_tag="OO330_002742"
     CDS             complement(16599..17384)
                     /locus_tag="OO330_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5883549.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17381..18049)
                     /locus_tag="OO330_002743"
     CDS             complement(17381..18049)
                     /locus_tag="OO330_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5883550.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(18113..18751)
                     /locus_tag="OO330_002744"
     CDS             complement(18113..18751)
                     /locus_tag="OO330_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5883551.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18764..20865)
                     /locus_tag="OO330_002745"
                     /pseudo
     CDS             complement(18764..20865)
                     /locus_tag="OO330_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20886..21512)
                     /locus_tag="OO330_002746"
     CDS             complement(20886..21512)
                     /locus_tag="OO330_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5883552.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(21968..22177)
                     /gene="fumD"
                     /locus_tag="OO330_002747"
     CDS             complement(21968..22177)
                     /gene="fumD"
                     /locus_tag="OO330_002747"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5883553.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5557 a         5739 c         5149 g         5747 t
ORIGIN      
        1 tgactgttgc aatggctgtg agggagaatc tttaagccat tgcgtttcag tggcattttt
       61 ccactcaacc agccaggaag ccattttccc gtaagggatg tatttattac cggcctcatg
      121 attctcattc tgacgtgcca gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg
      181 cctcatttca cactcctgtt gaccacctgg tcttaactgg aatttaaatg cctgtaatcg
      241 tttcacaggt caaatgatac tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag
      301 gcattgtgtt ttcctgatgc atgttcacct ggtctttgtc accaaatacc gacgccagat
      361 ttttgaccat gacacgacag aaaaactacg cacttacttt tcaaatgtat gtgctgattt
      421 tgcagctgaa ctggttgaaa tggatggcga accagatcac gtccatttgt taattaacta
      481 tcctcccaaa ctggcgatat ccagtctggt aaacagcctc aaaggcatat cgaataggtt
      541 actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg ttttgtggag
      601 tcctggctat tttgccagta gctgcgaagg tgcgccaata tccgtcatcc gccagtacat
      661 tgaacaacag caaacacctg gtcaggtgga aaaccgcgcc ttatatcccc gccctgaagg
      721 acgaggtttt acgacgcacc ggataaagga ctgacggatg cccgttcgca tccgtcagta
      781 ttgcaggacg gattattccg cgtccggctc ttcagacttg tatttagcgg cagtttcttt
      841 gatcagctgc tgcagttcgc cacgctgata catttcgatc acgatatcac aaccgccgac
      901 cagctcgccg tcaacccaca gttgcgggaa ggtcggccag ttagcatatt tcggcagttc
      961 cgcacgaatg tccggattct gcagaatatc aacataggca aaacgttcgc cacatgcggc
     1021 aagcgcctgg actgcctggg cagagaaacc gcagctcggc agtttcggtg aacctttcat
     1081 gtacagcagg atcgggtttt cagcaatctg gcgttggatt ttttcgatag tggtgctcat
     1141 tgtcttgctt cctcaaactt ctttacggca gtaatctgac attgtagcgg gtcagtgcgg
     1201 catcggaaaa taacattttc atcacgcttt tgctatttta tccctttgct ctatttgttg
     1261 catttcaaat attcgtttct tctctgatgc gcggcaaact ggcgtattta taaccatttt
     1321 tagctttcac tgctgcattt ttttgcgctc gccaacgaaa cgtatttttt aacaataaaa
     1381 gctattaact ttctcttctt ctatgcatta gaatcatcaa gttttgtaaa tcagacgcag
     1441 gcatgataga cctgccttaa cagagggacg ctcagtggcg cggataaacc gtatttcgat
     1501 cacgctctgt gctttacttt ttaccaccct gcctttaacg cctatggccc atgcttcaaa
     1561 gcaagccagg gagagttctg ctaccactca tatcaccaaa aaagcagata aaaagaaaag
     1621 cacggcaacc accaaaaaga cccagaaagc cagccagaaa acagcgaaaa aagccgccag
     1681 taaaagtatg accaaaagca aaaccgcttc ttctgttaaa aaatcttcca ttaccgcttc
     1741 taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa acggcctcag ccagctttac
     1801 cgaaaagtgt accaaacgta agggctataa atcgcattgt gtgaaagtca aaaatgccgc
     1861 gtcaggaacc cttgccgacg cgcacaaagc gaaggtgcaa aaagccacga aagtggcaat
     1921 gaataaactg atgcagcaaa ttggtaagcc atatcgttgg ggcggcagct caccgcgtac
     1981 cggttttgat tgcagcggcc tggtttatta cgcttataaa gatttggtga aaattcgtat
     2041 tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca ggacctatcg aacgtagtga
     2101 gctgaaaaac ggcgacctgg tctttttccg tactcaggga cgcggtacag ccgatcatgt
     2161 cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg cgcacaggtc aggaaattca
     2221 aatcacttct ctcagtgaag actactggca gcgccactat gttggcgctc gtcgggtaat
     2281 gaccccaaaa acacttcgct aaaactttac cctgttgtta cggcaacagg gtaagttcat
     2341 cttttgtctc accttttaat ttgctaccct atccatacgc acaataaggc tattgtacgt
     2401 atgcaaatta ataataaagg agagtagcaa tgtcattcga attacctgca ctaccatatg
     2461 ctaaagatgc tctggcaccg cacatttctg cggaaactat cgagtatcac tacggcaagc
     2521 atcatcagac ttatgtcact aacctgaaca acctgattaa aggtaccgcg tttgaaggta
     2581 aatcactgga agagattatt cgcagctctg aaggtggcgt attcaacaac gcagctcagg
     2641 tctggaacca tactttctac tggaactgcc tggcaccgaa cgccggtggc gaaccgactg
     2701 gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt tgccgatttc aaagcgcagt
     2761 ttactgatgc agcgatcaaa aactttggtt ctggctggac ctggctggtg aaaaacagcg
     2821 atggcaaact ggctatcgtt tcaacctcta acgcgggtac tccgctgacc accgatgcga
     2881 ctccgctgct gaccgttgat gtctgggaac acgcttatta catcgactat cgcaatgcac
     2941 gtcctggcta tctggagcac ttctgggcgc tggtgaactg ggaattcgta gcgaaaaatc
     3001 tcgctgcata ataactgatg gcaaatgcag cattgcctga tgcgcttcgc ttaccatgcc
     3061 aacgcggatg aggcgttaac agcgcattcg gcaagataaa tcgcacgttg tcagcaactg
     3121 taacgcagaa ggttatcctt ctgcgttttt gtttaattag ctgttagcaa cgcaaactgt
     3181 ttcaggttgt tttctggctg acataaacac cagcaataat gccagtcccg cgacaatcgc
     3241 ccccgtcacc ggcacaaagc tgtatcccag cccagcggaa attaccgcac caccagcagc
     3301 tgctcccagc gcatttccaa gattaaaggc accaatattg actgatgaag acagacctgg
     3361 cgcttcactg gcgacatgca tcacgcgcat ctgtaacgga ggtacaaccg caaaggttgc
     3421 tgcgccccac accaccatgc taatagctgc gccgaactca ttgcgggcca ggaacgggat
     3481 tgccagcata atcaccatca gcagcaacaa aaagcctttc aacgtgccgt taactgaacg
     3541 atctgccagt ttgccgccga gatagttacc gatagagaat ccgacaccaa tcagcaccag
     3601 cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt tgcagtaccg gagagatata
     3661 ggtgtagagg gtaaacattg caccagctcc cagtaccgtc gtcagcaatg cagacagcac
     3721 ctgcggacgc attaataccg ccagctcttt tttcacttca ggtcgtgccc ctgtaccacc
     3781 tttaggtaat gagaagaaca gacttaccat tgaaatcact cccagccccg ccgttgccag
     3841 aaatgacatc cgccagccga tggtttcacc caaccaggtc gccgccggca cgccaccgat
     3901 atttgccagg gttaacccca taaacatagt ggcaactgcg ctggcctgtt tatgttttgg
     3961 caccacgctt gcggccacga ctgaacccaa accaaaaaat gctccgtgat tcaggctggt
     4021 caaaatacgt gaaagcatca gggtcatata atccggcgcg atggcggaaa gaacgttgcc
     4081 tagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg cgggcacgat gagaaagtag
     4141 aagcgtcatc agcggcgcgc ccaccattac gccaactgca taggcactga ttaacattcc
     4201 ggcagcggga atcgagacat ccacaccgcg cgcaatgacg ggcaacaagc ccattggcga
     4261 gaactccgtt gtcccgatac caagcgcgcc aatcgccagc gccagcaacg gatagttaat
     4321 tttcatgcct tatctccacc tcttcgcgtc attacgcgat attcattaaa gtggcgaaag
     4381 catgacagca atcacaaaaa aatgaaaata acaaaaagag aaaacacttt tgccattttg
     4441 ctaacaaaca ggaaggagat gcgagggaga acgcgctccc tcgagaggaa atcagtgcag
     4501 cgcggcagtc aaacccacgg ctacgatcaa accgaggacg ataatcgttg ttaccagtga
     4561 aaatttaagg tcggtgctca tcaagttttc tcctttttta ttaccacaca aaaagtgata
     4621 ttacgcattt ttacacactg tgatgaaaaa atctcccgtc atttataatg ataagtgttt
     4681 ttaccacttc cccttttcgt caagatcggc caaaattcca cgcttacact atttgcgtac
     4741 tggccattga ccccttcctg acgctccgtg tcgtttttcc ggcgtaccgc aacacttttg
     4801 ttgtgcgtaa ggtgtgtaaa ggcaaacgtt taccttgcga ttttgcagga gctgaagtta
     4861 gggtctggag tgaaatggaa tggcaacaat aaaagatgta gcgaaacgag caaacgtttc
     4921 cactacaact gtgtcacacg tgatcaacaa aacacgtttc gtcgctgaag aaacgcgcaa
     4981 cgccgtgtgg gcagcgatta aagaattaca ctactcccct agcgcggtgg cgcgtagcct
     5041 gaaggttaac cacaccaagt ctatcggttt gctggcgacc agcagcgaag cggcctattt
     5101 tgccgagatc attgaagcag ttgaaaaaaa ttgcttccag aaaggttaca ccctgattct
     5161 gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat ctgtcgatga tggcgcaaaa
     5221 acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca gagccgttgc tggcgatgct
     5281 ggaagagtat cgccatatcc caatggtggt gatggactgg ggtgaagcaa aagctgactt
     5341 caccgatgcg gtcattgata acgcgttcga aggcggctac atggccgggc gttatctgat
     5401 tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg ctggaacgta acaccggcgc
     5461 aggccgcctt gccggtttta tgaaggcgat ggaagaagcg atgatcaagg tgccggaaag
     5521 ctggattgtg cagggtgact ttgaacctga atccggttat cgcgccatgc agcaaatcct
     5581 gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc gatatcatgg caatgggcgc
     5641 actttgtgct gctgatgaaa tgggcctgcg cgtcccgcag gatgtttcgc tgatcggtta
     5701 tgataacgtg cgcaacgcgc gctattttac gccggcgctg accacgatcc atcagccaaa
     5761 agattcgctg ggtgaaacag cgttcaacat gctgttggat cgtatcgtca acaaacgtga
     5821 agaaccgcag tccattgaag tgcatccgcg tttgattgaa cgccgctccg tggctgacgg
     5881 cccgttccgc gactatcgtc gttaatcacc cgttgcggga gtctcttccg gctcccgcag
     5941 ccactcctta ttcagcgtct cactatcgcc gagatactca agcagccagg ttaacgcagg
     6001 cgacatatca ttttgctgcc atgtcagaca acatgccgaa tccgggaagg ggttttccag
     6061 ttcaagtgct acccacttcc cctcattaag ccacggtttg gcgaaatgtg ttggcaccat
     6121 ccctatgcat aatcctgccg agatacaggt tgccgatgat tcccagtcag gtacgacaag
     6181 tcttttttgg ttatccagca accaggtaat acgtttaggt agcgttcgcg aggtgtcttc
     6241 gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg aacgggccat ccatcaacgc
     6301 cagcgggtgg tgactggcaa caacgcaact ccagcttagc atccccatat cccggaaggc
     6361 ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatt gccagttcca cgcgcccgtc
     6421 ggaaagcgca tcccagacac cgttgaacac ttcctgaaag acaagaagtt cgacatcatc
     6481 aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt tctggcctga caatattatc
     6541 cactgcgata gctaactgac cgcgccagcc gttcgctatc tgctgacatt gctggcgggt
     6601 gatctgcatt tttttgacaa cagagcgccc ttctttgaga aaccacgctc cagcagcggt
     6661 cagctccaca tcacggtgcc gtcgttcaaa gagcggtacc gccagccact cttccagctg
     6721 acgcacggta tagctgaccg cagaaggaac acgatgcagc tcctgtgccg cagcgctaaa
     6781 actaccatta cgcgctaccg catcaacaac ttcgagtgaa tattctgacc acatagtctg
     6841 cctgcaaaat ttttgaaacc agtcatcaaa tattaccgtt tcacaacact aatttcactc
     6901 cctacacttt gcggcggtgt ttaattgaga gatttagaga atatacatgc aacctgggaa
     6961 aagattttta gtctggctgg cgggtttgag cgtactcggt tttctggcaa ccgatatgta
     7021 tctgcctgct ttcgccgcca tacaggccga cctgcaaacg cctgcgtctg ctgtcagtgc
     7081 cagccttagt ctgttccttg ccggttttgc cgcagcccag cttctgtggg ggccactctc
     7141 cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg acaatttttg cgttaggtag
     7201 tctggggatg ctgtgggtag aaaacgccgc cacgctgctg gtattgcgtt ttgtacaggc
     7261 tgtgggggtc tgcgccgcgg cggttatctg gcaagcatta gtgacagatt attatccttc
     7321 acagaaagtc aaccgtattt ttgcgaccat catgccgctg gtgggtctgt ctccggcact
     7381 ggctcccctg ttaggaagct ggctgctggt ccatttttcc tggcaggcga ttttcgccac
     7441 cctgtttgcc attaccgtgg tgctgattct gcctattttc tggctcaaac ccacgacgaa
     7501 ggcccgtaac aatagtcagg atggtctgac ctttaccgac ctgctacgtt ctaaaaccta
     7561 tcgcggcaac gtgctgatat acgcagcctg ttcagccagt ttttttgcat ggctgaccgg
     7621 ttcaccgttc atccttagtg aaatgggcta cagcccggca gttattggtt taagttatgt
     7681 cccgcaaact atcgcgtttc tgattggtgg ttatggctgt cgcgccgcac tgcagaaatg
     7741 gcaaggcaag cagttattac cgtggttgct ggtgctgttt gctgtcagcg tcattgcgac
     7801 ctgggctgcg ggcttcatta gccatgtgtc gctggtcgaa atcctgatcc cattctgtgt
     7861 gatggcgatt gccaatggcg cgatctaccc tattgttgtc gcccaggcgc tgcgtccctt
     7921 cccacatgca accggtcgcg ccgcagcgtt gcagaacact ctgcaactgg gtctgtgctt
     7981 cctcgcaagt ctggtagttt cctggcttat tagtatcagc acgccattgc tcaccaccac
     8041 cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt tacatgatgc aacgttgtga
     8101 agaagttgac tgcccgaatc atggcaatgc cgaagtcgct catagcgaat cacactgatc
     8161 tatatcgata tacttatact taggctgcta acaaaatttt gttgtatgat tgaaattagc
     8221 ggcctatact aatttcgagt tgttaaagct acgataaata ttatgttttt acggggacag
     8281 gatcgttccc gactcactat ggatggtcat ttcggcaagg gttcctcctt tccctctgtt
     8341 ctacgtcgga ttatagactc gcggtttttt ctgcgagatt tctcacaaag cccaaaaagc
     8401 gtctacgctg ttttaaggtt ctgatcaccg accagtgatg gagaaactat gagttcatcg
     8461 tgtatagaag aagtcagtgt accggatgac aactggtacc gtatcgccaa cgaattactt
     8521 agccgtgccg gtatagccat taacggttct gccccggcgg atattcgtgt gaaaaacccc
     8581 gattttttta aacgcgttct gcaggaaggc tctttggggt taggcgaaag ttatatggat
     8641 ggctggtggg aatgtgaccg actggatatg ttttttagca aagtcttacg cgcaggtctc
     8701 gagaaccaac tcccccatca tttcaaagac acgctgcgta ttgccggcgc tcgtctcttc
     8761 aatctgcaga gtaaaaaacg tgcctggata gtcggcaagg agcattacga tttgggtaat
     8821 gacttgttca gccgcatgct tgatcccttc atgcaatatt cctgcgctta ctggaaagat
     8881 gccgataatc tggaatctgc ccagcaggcg aagctcaaaa tgatttgtga aaaattgcag
     8941 ttaaaaccag ggatgcgcgt actggatatt ggctgcggct ggggcggact agcgcactac
     9001 atggcatcta actatgacgt aagcgtggtc ggcgtcacca tttctgccga acagcaaaaa
     9061 atggctcagg aacgctgtaa aggcctggat gtcaccattt tgctgcaaga ttatcgtgac
     9121 ctgaacgacc agtttgatcg tattgtttct gtggggatgt tcgagcacgt cggaccgaaa
     9181 aattacgata cctattttgc ggtggtggat cgtaatttga aaccggaagg tatattcctg
     9241 ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg atccctggat taataaatat
     9301 atttttccga acggttgcct gccctctgta cgccagattg ctcaatccag cgaaccccac
     9361 tttgtgatgg aagactggca taacttcggt gctgattacg atactacgtt gatggcgtgg
     9421 tatgaacgat tcctcgccgc atggccagaa attgcggata actacagtga acgctttaaa
     9481 cggatgttta cctattatct gaatgcctgc gcaggtgctt tccgcgcccg tgatattcag
     9541 ctctggcagg tggtgttctc acgcggtgtt gaaaacggcc ttcgagtcgc tcgctaaagg
     9601 ctattctatc gccccctctt cgggggcgag ttcagatcag gcttctgtac ctggttgatt
     9661 catggcattt tctcgtgccg ccagcacacg ttctaccgta tctaccactg cctgagtttg
     9721 tggatcgatt tcaatgttga cgcgtgcgcc aagttttttc ttcccaagag tcgtgcgttc
     9781 cagtgtttcc ggaattaaat ggacgcaaaa acgcgttggc gtgacttcgc cgacggtcag
     9841 gctaataccg tcgatgccaa taaatccttt gtacaggata tatttcatca actgactatc
     9901 ctggacttta aaccagatct ggcgattatt ttccgaggtt aatattttcg ccacttcagc
     9961 ggtggtcata atatgacctg acattaagtg tccgccaatt tcatcactga atttcgccgc
    10021 acgctcaacg tttacccaat cccccacttt taaatcgcca agattggtaa tgcgtaacgt
    10081 ttctttcatc aggtcaaaac tgacatggtt gccgttaatt tccgtcacgg tcaggcagca
    10141 accgttatgc gccacggaag caccggtttc caggccgtcc agcatgtggt cgggtaactc
    10201 caccacatgc gtacgaaaat ttggtttctc gtcaatcgac accagttttg cggtgccctg
    10261 tacaatcccc gtaaacatac ttacaactcc tgaaatcagt taagacattc tgttcagcac
    10321 aatagcaggt ggaaaacgcc cttaccagtg aaggggtaag aatggctatt ttttcactgg
    10381 agaattaata aatcctcgct acaatagact gaatttcccc tacttcttct ttttgctgcc
    10441 cattcaggcg gctttttagt ctctcatata actacaaata aaaggtgttc acgtgcagaa
    10501 gtatatcagt gaagcgcgtc tgttattagc attagcaatc ccggtgattc tcgcgcaaat
    10561 cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg ggcggctata gtgccaccga
    10621 catggcggcg gtcgctatcg gtacttctat ctggcttccg gcgatcctct ttggtcacgg
    10681 actgctgctg gcattaacgc cggttatcgc gcaattaaat ggttccggtc gacgtgagcg
    10741 cattgcgcat caggtgcgac aaggtttctg gctggcaggt tttgtttccg ttctcattat
    10801 gctggtgctg tggaatgcag gttacattat ccgctacatg gaaaacatcg atccggctct
    10861 ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg ggcgcgccgg gatatctgtt
    10921 cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa accaagccgg gtatggtaat
    10981 gggctttatc ggcctgctgg tgaacatccc ggtgaactat atctttattt atggtcattt
    11041 cggtatgcct gagctcggtg gcgttggttg tggcgtggct actgcggcgg tgtattgggt
    11101 catgttcctt gccatggttt cttacattaa acgcgcccgc tccatgcgcg atattcgtaa
    11161 cgaaaaaggc accgctaagc ccgagcctgc ggttatgaaa cgactgattc aactcggttt
    11221 gccgattgcg ctggcactgt tctttgaagt gacactgttt gccgtcgtgg ctctgttagt
    11281 gtctccgctc ggtattgttg atgtcgcagg acaccagatt gccctgaact ttagttcact
    11341 gatgtttgtg cttccaatgt cgctggcggc agcggtaact atccgcgtag gttatcgtct
    11401 gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg accgggctta tggtgggtgt
    11461 ctgtatggca accctgacgg ccattttcac ggtttcactg cgggagcaaa tcgccctgtt
    11521 gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat ttgatgttgc tggcggcggt
    11581 atatcagatt tctgactcaa tccaggtgat tggcagtggg attttgcgtg gttataaaga
    11641 tacgcgttcc attttctata tcacctttac agcttactgg gtgctgggct tgccaagcgg
    11701 ctatattctg gcactgaccg atctggtcgt tgaacctatg gggccagcag gcttctggat
    11761 aggctttatt atcggcctga catcggcagc cattatgatg atgctgcgta tgcggttcct
    11821 gcaacgtatg ccgtcagcca tcattctgca acgagcgtcc cgctaataaa gacaaggcgc
    11881 aaccttcacg ggttgcgcct gtgtttttac gcaggctgga gcgttgcgcc aatcccgtct
    11941 tcgtctggct gtaatttcag agcgttacac agagtttgcc gactggcatc tgttatctca
    12001 acgcgtatat ccccgttaac ttcccctttc acattgatga agtcaggtga aatttctttc
    12061 gcactggttt gcaggcaaat cgctccctct ccgcgcaggc gtttcacttt cagttgcaca
    12121 tgcgttgccg caccagtgat cagccacagt tttccgccat tcagattgat gttaccaaca
    12181 atgaaagtga cgctgtcacc ttttaccgta ctgccgtcga gaatcaacac accgccctgc
    12241 tgtacagtaa tcgttgattg ttgcagctct gcgccataac caataatcat tgaagcgccg
    12301 tccagtaatg caacactccc ggtcagttca accggtgcgc gatcttcctc tacgacaaaa
    12361 ttttcgtccg cgctggtggg tgacaacagg atcactgttc caccttgtat taccgcattt
    12421 tccagcgtac cggcatgcac ttcagcccgc ccccccacat tcaccgacag gttttgcgtc
    12481 ttaccggaac tgtagagctg aaggccatcc gtccccagac gataaatgga tccattctca
    12541 acaatagtgt cagagtcttc accaccgaga ttattcatgg tgccaccagc aagcaacgta
    12601 tcactggctt tcccggccag cataaaaacg ccgccctgat taatctcaca attgctggct
    12661 tctccacttg gatacaccaa caattcacca ccttcatcta ccacgaccgc tgaggttgtc
    12721 ccattaacca acaggccgcc ctcttgatcg agaatgattt tttccgcgcg atgtccttcc
    12781 agtacacgca ggttaccgcc attttccagc aacaaaccgc aggcataacc ctgatcaacg
    12841 ctgaattcac cttcgggatg gcggccatta accgtagcga gcgtagagag actaattgcg
    12901 cctccctcgg actgaacaat atctgtggca atggcaccgt cgctgaccga tagcgtgcca
    12961 ttgttaccgt actgtgtgca tttggcgatc ccaccggttg ccactttcaa ccagccagcc
    13021 cccgtgacca cggtattttc ggctcttccg ccagcttcaa caatcaactc gccttcagta
    13081 agttgcgtcg cgccaaggac ctcgccggtc ttaacaattt gcacattccc gtcgctcatc
    13141 aagtttttcg catcagatcc catgatttat tcctttgctg catctgtgtg cctttattgc
    13201 tacctaagtg taaaggctac ggaggattta tccacgacag atttgagatg gtggcaaaca
    13261 actctgttta aactctgata cacgaattat tgggttgtat cagatgtaaa tgcgatcctg
    13321 aataaaaatc gcccttgcaa atcaacaaaa tatagccaat ttgcttaaaa gctctgcaaa
    13381 cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc acctttccac tctgccgcta
    13441 atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag ctcagttggt tagagcacca
    13501 ccttgacatg gtgggggtcg ttggttcgag tccaattgaa cgcaccatcc tgcgtccgta
    13561 gctcagttgg ttagagcacc accttgacat ggtgggggtc ggtggttcga gtccactcgg
    13621 acgcaccaga ttttcttaat ctggtctttt cctttttccc tctgtttctt ctctgtatcc
    13681 aatacgttaa aagatttaca ctgtcttcgt atgcgttatc agaaggagaa tcgctatggc
    13741 aactttgtta caacttcatt ttgcttttaa tggcccattt ggcgacgcaa tggctgagca
    13801 gcttaaacca cttgctgagt cgattaatca ggaacctggt tttctgtgga aggtatggac
    13861 agaaagtgaa aagaaccacg aagccggtgg gatctacctt ttcactgatg aaaaaagcgc
    13921 ccttgcctat ctggaaaaac atactgcccg actgaaaaat ctcggcgttg aggaagttgt
    13981 cgccaaagtt ttcgatgtca atgagccact tagtcaaatc aatcaggcaa aactcgcctg
    14041 acagaattta atcaagggcg gttagcgccc ttttcatccc tgtctgaaat ttctcaaatt
    14101 ctaaaaatct caaccaaact tatctgataa cactaaattc gaaagaatgc gtacaggtaa
    14161 gtaacaatga aaaaaattgc tattgtgggt gccgggccta cggggatcta caccttattc
    14221 tcgcttctac agcaacaaac tccactttct atttctatct tcgagcaggc tgacgaggcc
    14281 ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa tgatgctggc aaatattgcc
    14341 agtattgaaa taccgccgat taattgtacg tatctcgaat ggctacaaaa gcaagaagcc
    14401 agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc acgatcgtca gtttttaccg
    14461 cgaattctgc tgggcgaata tttccgcgat caatttttac gattagtaga ccaggcacga
    14521 cagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg ttaccgatct gcaaattaca
    14581 aatgctggcg tcatgctcgc tacaaatcag gatttaccca gcgagacgtt tgatttagcg
    14641 gtgattgcca cgggtcacgt ctggcctgat gaagaagaag caaccagaac gtattttccc
    14701 agcccgtggt caggcctgat ggaagcaaag gtcgatgcgt gtaacgtggg tattatggga
    14761 acatccttga gcggactgga tgcggcaatg gcagtggcta ttcagcatgg ttcgttcatt
    14821 gaagatgata aacaacacgt cgtttttcac cgcgataacg caagtgaaaa gctaaatatt
    14881 acgttgatgt cgcgcacggg tattttaccc gaagccgatt tctattgccc tattccctac
    14941 gagcccttac acatcgtcac cgatcaggca ttaaatgctg agattcaaaa aggtgaagag
    15001 ggccttttgg atcgggtatt tagattgata gtagaggaaa tcaagtttgc tgatccagac
    15061 tggagccaac gcatagcctt agagagcctg aatgtcgatt cctttgctca agcctggttt
    15121 gccgagcgca aacaacgcga cccatttgac tgggcagaaa aaaatctcca ggaagtcgaa
    15181 cgcaataaac gagaaaaaca tactgttccc tggcgttatg tcattctgcg cctgcatgaa
    15241 gccgtacagg aaattgttcc tcatctgaat gaacacgacc ataaacggtt cagtaaaggc
    15301 cttgccaggg ttttcatcga taattatgcg gcaatccctt cagagtctat tcgtcgacta
    15361 ctggccttac gtgaagcggg gatcattcat attctcgccc tcggtgaaga ctacgaaatg
    15421 gaaattaatg agtcgcgcac cgtcctgaaa acggaagaca acagctactc gtttgacgtt
    15481 tttattgatg cccgcggaca gcgtccgctt aaagtgaaag atattccttt ccctgggcta
    15541 cgcgagcaat tacagaaaac aggggatgaa atccctgatg ttggcgaaga ttatacgtta
    15601 cagcaacccg aagatattcg tggacgcgta gcgttcggcg cgttgccctg gttgatgcac
    15661 gaccagcctt tcgttcaggg acttacggca tgtgcagaaa ttggtgaggc gatggctcgg
    15721 gcggtcgtaa agcctgcatc ccgtgctcgt cggcgtcttt cgtttgatta aaggtaaagc
    15781 tatcagcact caacaaactg atgctgttca ttaagacgat agcggactcc ggttcgaata
    15841 ttgttttcac cttcaatggc gacggcgaag cgaactcttt cgccgtcatg atatgccagt
    15901 gcggcgcttc cccctagccc gagaataatt gagtcgacca cgcccgtgct cactaccgtt
    15961 gaattttcac cgctggcaat gatgtgtaca ttgtcgccgc tgttggcaat tctggcgttt
    16021 gcgccaaagc tggcgatttg caccaggtca ccattactgg cgacatgaca ccgttcaccc
    16081 aatgtacaaa cgcgtactcg cattccggta ttcgcaatcc gactactatc tccagcgctg
    16141 cttatccgcg aagaattacc ggttacagca acccgtgcgc gttcaccgga gctgccaatg
    16201 tgactgttaa agccgacact tccaatccgc acgctataac ccatactggc aatttgcgcg
    16261 gcatacccag cggttgcgat tcgggcgtta tcctcacaac agccgatgcg agcatgatcg
    16321 tcgctgcggg caaactgact gcaaaatggt atctctccgg ggtctgtggc cagtttttgc
    16381 atagaggaaa cttcctgatg cgcgaagttt tcatccgcca gccatttttt ccacgcatac
    16441 tcaaccagac tctccgccca gtccgtatat ccttcctcaa tcagtgcatg acgaatatcg
    16501 gcataactac cgccgcgtgg gaaatggcgc aggaaccagc gatacatcac cgctccgata
    16561 cgccattctc gcaggtctgc tcgtgtaatg atcattcgtt agtgccggtg atagccatcg
    16621 accatgcttt taaaggtttc gtgtggcgta cgccccgtgg tgcaaagata aagatgaccg
    16681 aagataaaaa agaggcttat aaatgccaga gcaaaatgcg cctgcaataa ccagtatctt
    16741 acgccaggaa acacatctcc cacggcttgc ggatagagac acaacagccc cgtcaatagt
    16801 aacaacggca gcaatccata catgacacca acataggcga cctgctgtaa gggattaaat
    16861 ttagactgtg ttgttgccgg gaaaggatgt tcttccccct gcataatgcc aaacaaataa
    16921 aatcgcgttt gttttgccgc tcgttccagc cacccctgac gacgaatgcg atagtggtga
    16981 ccattatccc caacggcatt gatcagcaca aagccgagcc agcatgccag taacaaaaat
    17041 ccgcaaactt catgcaccgc aaccagactt ttaaccgcag ttgcgcccac catcgcaaag
    17101 tgatttatca gcccacttgc cagcaacaat acaaagagta acgcattcga ccagtgccat
    17161 aatctgaccg ctttgctgta aagataaacc ttctcaccat gatcggtggc tgatttcttc
    17221 acgccacgag cacgaagcaa ggcgtgcagc cccagcacca accacatccc aaccagcaat
    17281 actccggcaa tgatcaacca caccggccaa aattcgggag tgaactgtgg cacatagttc
    17341 gctaactggc tctgaaactg ttcagcatgt tgcgacgggt tcatacattt tcctttttaa
    17401 tcaaatgttg accgaaccgg cgatacagat gcggttttcc ggcaccaggt agctgatatt
    17461 gatagtattt attatcctgc aaccacgcct gaatttctgg gctatcttcg cgaccaaaga
    17521 tcaatgcgtg ttccggacag gcgctgacgc aaatgggcgg aaagcctttt gccagtcggg
    17581 actcagcgca gaaatcacat ttatctgcca ctttagtgac agggttaagg tagcgaacct
    17641 ggtacggaca agcgccaatg caataactgc agccgatgca ctgcgatttt tctacccgca
    17701 cgatcccctg ctcatcacgc cacgatgccc ccgtcgggca tacgtcgatg cacggagcat
    17761 cttcgcagtg ctgacatgac tggcgaaaaa agtgatactg cgtttcgttg tcgttatcgg
    17821 tgactggaat gtgtgctatc gataaacggc ttccctgagc ggggacgtgg ttagttttac
    17881 ggcatgcgcg ggcgcaaata ttacagccat tgcaccgtga ctcgtcatga atcatggcat
    17941 accgaacttc cttttcttgc ctcgtgttcg ctaacagaga agacgcagaa ccagtaaaaa
    18001 agattactgt tcccatcccc agaacaaatt tgcgtcgagt gaaggacatc tgattatccc
    18061 ctaatgcagt ggctgcaact gtccacccta tccatgacat ctccctgaaa tttcaggggt
    18121 tctgaaactg ctctacctcg aaccattcgc ataagccata agccttgcca taggtcataa
    18181 atcgctcgct aaacaagtac aacaccgccg tcgatgactc cacctgccgt atatcggcat
    18241 attcaggtgc aacatccatc gcggcgatcg cggcttcaat ctgcgcttcc tggaagtaat
    18301 atggcgcttg catcagcatg gcgactttgt aaggacgagg atacgtctgg cactcaaaac
    18361 gaacggcgtg tgcaatggcg cgacaaatgt cctgctccac aacttgcaaa gacatcgccg
    18421 cgtagttttc actcatggcc tgcgttgagt aatagtaatc atcctgactt cccgtcatac
    18481 aagcgatatc cgcgtagtct ccgttctgct taatgctttc cagcaaaact gacagttcct
    18541 cttctgcaac cgaatagggt ggcgagagaa acacggtgcg cgccaccagc tgcccagccg
    18601 cagaatgctg gcgaatgaac tcagccagga tttcagcagg ttctggttcg gttaccgtat
    18661 caacggggtg acgcatcccc tgcaaccatt gccgcttcgc ctcgtcaact tcgctgactt
    18721 tcgctaacgg caattcatcc tgatggttca tcttacccat tccttacgca ggcagtagat
    18781 tgtgtgctgc cagatcggcg gcaatatctt ccagccccag acgctgcaat gtttcgcggg
    18841 ttggacaacc aagctgtggg tcccagccca tttctttgta gaacatcgtg agcgaagcat
    18901 gcatatcgtc acgatccatt ttgtcagtac cttcagtaaa gaccgggatc tgcggatcct
    18961 tgtcgaaaac ccaggaacag ataagatcgt gttcattacg catatctttg gtttgcatcg
    19021 gttttaccgt gtaggcacga tgcaacgtaa agatacgctc tgcagctaaa tctaattttt
    19081 cctgagtcat ctcttcgccg gtgatcgctt tgaagaattt ggcttcaagc gccagatcgc
    19141 cccggtaatt acggcttttc agcggcgaaa cggtcattgg ccagacccag ttgcacagag
    19201 tgacggcgtt atgcaaacag acccgcaaca gcgaccactt tgcatattta atttttgcgt
    19261 cattgattgg cgtgtagttt ttggtttcat cgtaagcatc ttcagaacca aaaagttctt
    19321 tcgccacttc acgttgcagt ttcaatggca agccggaacc aataaagttg atatgggtat
    19381 gcgtcatgca gtcacggttg aacatacagt taacaatgga accgacctgc gctgacgctt
    19441 cattggcatg gtgaaccgga tagccaaacg gcgaccagag tttatttttc gcgtagcccc
    19501 agtactcttt acccaaattc cagcgttctg cgatggcata tgaaccatca gccaggtgac
    19561 tcagctcacc cacacgatgc gccagacggt agtaaaaatc tttaatgaag ttaacgtcac
    19621 ccgcttccag ttgatcccag cgaatttctg catactcttc agctggcaga acacgcttga
    19681 acacaccttt gctgtaacaa taagtaaagt cgcgatgcaa ctgcccgtag ttacaccata
    19741 ggccgtagtc atcgaacaaa ttcagaccaa ccaggttacc aatcacacga ccatcgtctt
    19801 tatcttcaaa atctttcggc ccgttcggga agatggtggt atggacaaag tttgctacac
    19861 aagtgttacc acctgtgctg ggcacgccaa actctttcac ccgaggaata ttcatttggg
    19921 tcatacaacg gatcgggcaa gagtggcagc cgctcatttt tactgtgtat ttctctgccg
    19981 ccggtcctaa gtcaaaaacg gatttatagg tacgaaagcc gaccgtattc tgattgcctg
    20041 gcggaatttc acccgtttca atcggaccac cttcagccgc gccccaaaac agccctttac
    20101 gtgctgtcca gcgtgacttg ggatctgaat actctgccca cgattgtggc gtacttggca
    20161 cgacatggtt gttattcgca ccaataagtt cagtcatcat gtaatcattg agacgcttca
    20221 tctcctgacg atcggcaatg ttgacccctt tcgtcccttc aaccgcaatc gctttcaggt
    20281 ttttcgaacc cattattgcg ccagttcccg caccgccgct gtggttacgg ctattcaaca
    20341 tgccagagag aggaataagg ttttccccag cctgaccaat agccgccaca caggtttccg
    20401 ggctggtcaa tcgacaaatt tcttccgtcg ttgcgcgcgt cccttttccc cataagaaat
    20461 cggctttttc caggctaact ttatcatctt taatcttcag ccataccggt gatttcgcct
    20521 tcccttcgat aataatgacg tcgtatccag cgaatttcat ttgcgctgca aaaaagccac
    20581 ccatatgggc atcgcgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    20641 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    20701 cgcttcatcg aaaggtttcg tgcctagcgg tacttcgtca tacataattt tgtagccgaa
    20761 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    20821 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    20881 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    20941 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    21001 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacaacg tttatgatcg
    21061 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    21121 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    21181 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    21241 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    21301 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    21361 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    21421 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    21481 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    21541 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    21601 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    21661 taatccttta tggttaggag aaaaaataat gtgattattc tgagccctta acattgatcg
    21721 ttatcaatta aaattacaaa gcaagaaatt ataagcgaac tgatatttat tatcatttga
    21781 aataaattta acttaagcga caatcatttg tgacgttcat tgcagaaaat aaaaataaca
    21841 ttatcgctat aaattaaaat acataccgaa attcgtataa tttcagcagt tgttatttag
    21901 aatgattaac tggaccgcaa ctgaagcaag aatatcagga tgaagcttac cgcctcatcc
    21961 tgaatggtca ttttaccagt gcgttaatga cggtttccat cgcctgcttt tccagctcac
    22021 tgcgctgaat gcgtttattg gctaatgctg tacgtaatac gcctgcgata acaatatgct
    22081 ttggttcctg ccccagatcg cgcatttcca gcacaacttt accgacaact ctgcacattt
    22141 ctcggtacag ttcatcctct tttgtccgat ttcccattct ttatacctat tt
//
LOCUS       DAJTRB010000068        21519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTRB010000068 DAJTRB010000000
VERSION     DAJTRB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.068"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO330_002748"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO330_002748"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5883554.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTSVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="OO330_002749"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="OO330_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5883555.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="OO330_002750"
     CDS             complement(2650..3201)
                     /locus_tag="OO330_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5883556.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="OO330_002751"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="OO330_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5883557.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSVSSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5216
                     /gene="cybC"
                     /locus_tag="OO330_002752"
     CDS             4830..5216
                     /gene="cybC"
                     /locus_tag="OO330_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5883558.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5261..5725)
                     /gene="nrdG"
                     /locus_tag="OO330_002753"
     CDS             complement(5261..5725)
                     /gene="nrdG"
                     /locus_tag="OO330_002753"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5883559.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5883..8021)
                     /gene="nrdD"
                     /locus_tag="OO330_002754"
     CDS             complement(5883..8021)
                     /gene="nrdD"
                     /locus_tag="OO330_002754"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5883560.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSIFTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8415..10070)
                     /gene="treC"
                     /locus_tag="OO330_002755"
     CDS             complement(8415..10070)
                     /gene="treC"
                     /locus_tag="OO330_002755"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5883561.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10120..11541)
                     /gene="treB"
                     /locus_tag="OO330_002756"
     CDS             complement(10120..11541)
                     /gene="treB"
                     /locus_tag="OO330_002756"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5883562.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11660..12607)
                     /gene="treR"
                     /locus_tag="OO330_002757"
     CDS             complement(11660..12607)
                     /gene="treR"
                     /locus_tag="OO330_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5883563.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12792..12845
                     /gene="mgtL"
                     /locus_tag="OO330_002758"
     CDS             12792..12845
                     /gene="mgtL"
                     /locus_tag="OO330_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5883564.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12986..15682
                     /gene="mgtA"
                     /locus_tag="OO330_002759"
     CDS             12986..15682
                     /gene="mgtA"
                     /locus_tag="OO330_002759"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5883565.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15888..16274)
                     /gene="ridA"
                     /locus_tag="OO330_002760"
     CDS             complement(15888..16274)
                     /gene="ridA"
                     /locus_tag="OO330_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5883566.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16347..16808)
                     /gene="pyrI"
                     /locus_tag="OO330_002761"
     CDS             complement(16347..16808)
                     /gene="pyrI"
                     /locus_tag="OO330_002761"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5883567.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16821..17756)
                     /gene="pyrB"
                     /locus_tag="OO330_002762"
     CDS             complement(16821..17756)
                     /gene="pyrB"
                     /locus_tag="OO330_002762"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5883568.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17760..17894)
                     /gene="pyrL"
                     /locus_tag="OO330_002763"
     CDS             complement(17760..17894)
                     /gene="pyrL"
                     /locus_tag="OO330_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5883569.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18175..18570)
                     /locus_tag="OO330_002764"
     CDS             complement(18175..18570)
                     /locus_tag="OO330_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5883570.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18702..19415)
                     /gene="bdcA"
                     /locus_tag="OO330_002765"
     CDS             complement(18702..19415)
                     /gene="bdcA"
                     /locus_tag="OO330_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5883571.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19486..20079
                     /locus_tag="OO330_002766"
     CDS             19486..20079
                     /locus_tag="OO330_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5883572.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20224..20676
                     /gene="tabA"
                     /locus_tag="OO330_002767"
     CDS             20224..20676
                     /gene="tabA"
                     /locus_tag="OO330_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5883573.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20799..>21519
                     /locus_tag="OO330_002768"
     CDS             20799..>21519
                     /locus_tag="OO330_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5883574.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5204 a         5324 c         5663 g         5328 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 acgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgccgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgt atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaagtaaaa gaagcgcagg
     5161 ctgctgcaga gcaactgaaa acgacccgca acgcctatca ccagaagtat cgttaattcc
     5221 tcatttccct gttgcctgca ctcaggtaac agggaactta tcatcgcaaa tgatgcacca
     5281 cctgattgct gctgccgcgc cagatcaggg aagggtcttt taaatcctgc acaaatttgc
     5341 cgtcgaccag cacgttaatc aaatcaacaa cctgcatttg cgcagcgttg agttcgtcga
     5401 gtttatagcc tgtccacacc cagatgtctt tacccggaca ctcggcgcgg atgcgtttta
     5461 ccagtttcag aatatccggc acgttttgcg gatgcagcgg atcgccgccg gagagggaaa
     5521 tcccctggcg tttgatacga gtgtcattca gatctttaat gatcttgtct tccattgctt
     5581 tggtaaatgg ctgaccagaa tttacccgcc atgtgctttt gttatagcaa ccggggcatt
     5641 catgaacaca cccggagaca aacagggtgc agcgagtgcc ggggccgttg acgatgtcga
     5701 caggatagta ctgatgataa ttcattattt cgcctggctt atcagtccgc gtgtaggctg
     5761 catccggtaa tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta cagccgttgc
     5821 caaatgtagg ccggataagg cgtttacgcc gcatccggca tttgcttagc gcagagtgaa
     5881 gattaaccta tctgcccatt ccccaaatgt ttaacgcggc gcttaacttc ttcctgctta
     5941 ccagcgttaa acggacgtgc atccgggcta cctaaatatc cgcacacgcg gcgagttacc
     6001 gacacacggg aggcgtcatg gttaccacat ttcgggcaag tgaagccttt gctggtgcac
     6061 tcgaactcac cggtaaagcc acactcgtag cactcatcaa tcggtgtatt ggtgccgtaa
     6121 tacggtacat gctgatagct gtaatcccag acatcttcca gcgctttcag gttgtgctga
     6181 atgtttggat actcgccgta gcaaatgaaa ccaccgttcg ccagcggcgg gtaaggcgct
     6241 tcaaagtcga tcttgtcgta cgggttcacc ttcttctcca catcaaggtg gaaactgttg
     6301 gtgtagtaac ctttatcggt cacgcccggc accacgccaa actcagcagt atcgagacgg
     6361 cagaagcggt cgcacaggtt ttcactcggc gtgctgtaaa gactgaaacc ataacccgtt
     6421 tcttctttcc actgatccac tgcctgacgc agacgttcaa caatggcgat acctttcgcg
     6481 cgaagcttct cgttgtcgta aacatgctcg ccgctgaaca gcgcgttaat ggtttcgtgg
     6541 atgccgatgt aacccagcga aatagacgca cgaccgtttt tgaagatttc agaaacatca
     6601 tcgtcagcat tcagacgtac gccacaagca ccttccatat agaggatcgg ggcgacgcgc
     6661 gctttcacgc cttcgagacg agcgatacgg gtcatcagtg ccttacgtgc cagcaccaga
     6721 cgttcatcca gcagcttcca gaaggtggct tcatcgcctt ttgcttccag agcaatacgc
     6781 ggcaggttca ggctgatcac gccgaggttg ttacgaccat cgtggatctg ctcgccgttt
     6841 tcattttccc acacgccgag gaagctgcgg cagcccatcg gggttttaaa cgaaccggtg
     6901 acgttcacta cctgatcgta gttcaggata tccggataca tgcgcttgct tgcgcactcc
     6961 agcgccagct gtttgatgtc gtagttcgga tcgccttttt tatggttcag gccatcgcga
     7021 atcgcaaaca ccagtttcgg gaacaccgca gttttacggt ttttaccgag gcctgcgata
     7081 cggttacgca ggatcgattc ctgaatcagg cgcgattccc agctggtgcc cagaccaaaa
     7141 ccaaaggtta caaacggcgt ctgaccgttg gcggtgtgca gggtgtttac ttcgtactcc
     7201 agcgactgga aggcatcgta gcactctttg atggtacgag agttagcgta gccttcggcg
     7261 tccgggatgt tccactcttc tgcggtcttg cgatgtttgt tgtagctggc ggtaacaaac
     7321 ggcgccagca cttcatcgat acggttaatg gtggtgccgc cataaatatg gctggcgacc
     7381 tgagcaataa tctgcgcagt taccgcggtt gccgtaaaga tcgacttcgg cggttcaatc
     7441 tcggcgttcc ccattttaaa accctgggtc agcatgcctt tcaggtcgat caacatgcag
     7501 ttgaacatcg ggaagaacgg cgagtaatcg agatcgtgat agtgaatatc gccacgctca
     7561 tgtgcctgca ccacgtcacg cggcagcagg tgctggcgtg catagtgttt agccacgatc
     7621 ccggccagca ggtcgcgctg ggttggaatc accttgctgt ctttgttggc gttttcgttg
     7681 agtaacgagg cgttggtctg ctcgaccaga ccacggatct cctggttcag gcgaccgcgt
     7741 ttttcacgtt caatgtcgcg atcgtgacgg tactcgatgt aagcacgagc cagttgtttg
     7801 tatggacccg acatcagctg gttttcaact gcggtctgga tctcgttgat atccacctgg
     7861 ttgcgcccct gcatctgctc gttgacaacc gcggcaacag tggcgcaata atcggcatca
     7921 tcgacttccg ctgctttagc tgcacgcaga atcgcttctt tgatgcgctc tgatttaaac
     7981 ggcactttgc agccgtctcg tttcatcaca tgcggtgtca tgatcgctcc atatttttaa
     8041 gaacaggtta tccacagaaa ttgggaaagg cgttttccgg ttttttcgtt tcgttttccg
     8101 gtgctttccg caatcgccat ccgcttatcc acaacgccgc acctttttcg tggggagctg
     8161 tagtagcaat tatagtcgat taatacaaca tattgggttg ggatgcattt taaagtctat
     8221 atatagtgct ttgcatcaag gatgttttaa ctttttttga tgtagctcaa agtaaaaagc
     8281 agagcgtacg gatgacgggc gctacagcgt tatgtaaatt ttttaatgaa tttgctggtt
     8341 gaaaaatcaa caaaaacaac atgctgacag acaaaacccc ggaatgaccg gcattatcgg
     8401 ggcttaggga agatttactt ctgtaaccac cagacagcct caaaaggccg taaattcatg
     8461 gcacagggtt gtggtgaggc ttcttcgtag ttatgcatca caagctgcca gttgccgcgc
     8521 atttgccctg gctgccaggg ttggatctca cggctaaggt tggcaatgac cagcaaggtt
     8581 tgccccttcc attcacggcg atagcaccac aatacagggc tgtttggcag cagatcctgg
     8641 taattgcccc atgtcaggat ggcttcttgc ttacgcagtg cgattaactt ttggtaggtg
     8701 taaaacacag aggagtcatc ggccagcgcg gcttctacgt tgatttgttg atagttatcg
     8761 cctaggccaa tccacggttc gccagccgta aacccggcat tatcgccgtt gctccattgc
     8821 atgggcgtgc ggctgttgtc acgggattta ctggcgagga ttgccaataa ctcgtcggca
     8881 tcacgaccat cgttgcgcag ctcggcaaac atattgaggc tctccacgtc gcgatagtca
     8941 gtaatgcgcg tgaaatgcgg gttggtcatg ccaatctctt cgccctggta gatatacggc
     9001 gttccctgca tgccatgcag caccatcgcc agcatttttg ccgcaggcac gcggtattca
     9061 ccttcatcac caaagcgaga aacaatgcgc ggctgatcgt ggttacacca gaacaaggca
     9121 ttccatgcca cgttgtgcat tccttgttgc cagtggcgga acaatgtttt caacgccaca
     9181 aagtcaggtt tagccagcgt ccatttttca ccaccgggat aatcgacctt caggtgatga
     9241 aaattaaagg tcatcgacaa ttcactgcct gtcagtgccg cgtatcgctg gcaatgctca
     9301 aggctggtgg aggacatttc acctacggtc attaacccgc gtggcgtaaa cacatcgcgg
     9361 ttcatctcgt gcaaaaactc gtgtgctcgt ggcccgtcgg tgtagaagcg acgcccgtcg
     9421 ccatccaggt cttcagggaa acgcgggtct ttggagatca gattcaccac atccaggcgc
     9481 aacccgtcga ccccacgatc ggcccagaat tcacagactt ttttcagctc tgcgcgtacc
     9541 gctggattct cccagttgag atccgcctgt tctggtgcaa agagatgcaa atagtactgt
     9601 tcgctttccg catgccagcg ccacgcacta ccgccaaatt ttgaacgcca gttgttcggt
     9661 ggcgtttctg gttcgccatc gcgccagata taaaactggc ggtaagggct ttctttgttc
     9721 agcgcctcgc gaaaccaggc atgttgggta gaggtatggt taaacaccat atcgagaatg
     9781 atacgaatcc cgcgcgattt tgcctgcgtc accagttcgt caaaatcgtc cagcgtgccg
     9841 taggtgggat caatcgccgt atagttcgct acatcgtaac cgttatcgac ctggggagag
     9901 acataaaagg gggttagcca gatggcatca acgcccagtt tatgcagata gtccaggcgt
     9961 tggataacgc cacgtaaatc gccggtgccg ctacccgtgg tgtcctgaaa actctttgga
    10021 taaatctggt agataacgcc gttttgccac cagtggggaa gattagtcat tacgttattc
    10081 ctgcaaatgc gagggggcgc aattgcgccc cgaagaaaat taaacaatgt ccagcgtgcc
    10141 caggcggtat ttccgctgat agataaacga ggtgagtaca atcgggatga tgatggcgat
    10201 agccattgcc agcgcaaata cctgccagta gctcggttga atagagagaa ttcccggcag
    10261 gccccctacg ccgataccat tcgccataac gccgttcagg ccgcatagca atcctgccag
    10321 accagaacca atcatcgcgc acagcatcgg gaagcgatat ttcaggttga tgccgtacat
    10381 tgcaggctca gtgaccccaa gccaggcgga gatagcggca ggcacggaga tctcgcgttc
    10441 attgtgcttg cggctggaaa tgataatgcc tatcacggcg gagccctgag cgatattcga
    10501 cagcgcaatc agcggccaca ctggcgtgcc acccatgctt tgaatcatct gcaaatcaat
    10561 agcaagggtg gtctggtgta cgccggtgat caccagcggc gcgtacagga agccaaacaa
    10621 tgccgcacca atcggagcaa agctgccggt catcaggtga cgtaccgcaa aggcaacgcc
    10681 atcgccaatc atgcgaccaa acggaccaat cagcgcatgg gcgaggaaca ccgcgaggat
    10741 cagcgaacag acgggcacca ccaccagata gaggtaatcc ggtacgatac gtttaaggcg
    10801 agtttcaata acgcccagcg ccagcccggc taacagtgcc gggatcacct gcgcctgata
    10861 gcccactttg gcgatgctga acatgccaaa gtcccacact tccggcagct gctgcccgag
    10921 cagataagcg ttcatcagct ggggggaaac cagtgtcacg ccaagcacga taccaaggat
    10981 cggcgtgccg cccatttttt tcaccgctga ccagcaaatc ccgaccggca ggtagaagaa
    11041 aatcgcttca ccgatcaacc acagaaaatc gtagatcgtt tgcagggaag ggtacatttg
    11101 cgccagcgtc tgaccgttgc tcatgggcaa atcgccgatc acattgcgaa aaccgaggat
    11161 caaaccgccg ctaatcaacg cgggcagcaa cgggaagaag atctccgcga aatgagagat
    11221 caactgctca tgccatttca tattttgccg ggcggctttt tttacctgct ctttatcaac
    11281 ctgcgcctgt ccggttgacg ctattagtgc ttgatagtaa tcacccacgt tggtgccaat
    11341 caccacctga aattgcccgg cattggtgaa acagcctttc accatgggga gttgctcaat
    11401 ttctttcggt ctggcattgg ccggttggtt gaggacaaag cgcaggcgag taatacagtg
    11461 gctcaccgtc gcaatattgc cgcgcccgcc gaccagttca atcaaccgat cgatatccgt
    11521 ttggtttatt ttgctcatca taaagcccca tggcagatga catttttggt tggctgcaga
    11581 atatattgcg cggaaaaatt taaaaacggg aacgttcccg aaacgcagcg aagatcacaa
    11641 tttatcgttc aggaaacgat caggacaggg tggcggggat gatgatttgt tgcggttcgc
    11701 tgcgcccggt tacctgtgcg atcaattggc aggccgcctg gcgtccagct tcggcgtagc
    11761 caggatctac ggtgacgatc tccggatgaa ggaatttcat taacggcgta ttacctacgc
    11821 tcgccagttg caaggtgtcg atgcgttgct cttgcaggta tttacttgcg ccaagagcca
    11881 gcgtgtcggt tgcgcacagt aacgcggtag tttcaggcgt aatcactttt gctacgtttt
    11941 catagccttg cttcatagca agccctggca gagcggcaac gggatgcagt ttatgcgctt
    12001 tgcagaacgc caggtaggct tcgtgacgtc gcttcccggt cgtcacgtca ctatgcggca
    12061 cgccgagata actgatattg cgatgcccct ggtcatagag ccgttgcatc agaatcttga
    12121 ttgccccttc gtcgtcataa cagaccgaag caaagccttt tgcgtcacgc gccagcagaa
    12181 ccagcgatga ctgccagtgg gctaacattt cttcggttat gccggtaaaa ccgaacagca
    12241 ctacgccgtc gatattacgc cgtttcagca cccccaaatg ttcggcaact aattgtgggg
    12301 aaaactgatt ttccatcatg attggatcgt aaccttgttc atagaacgct ggcagcatgg
    12361 tttgaacggc gagattttct gacaacgaat ccagacgggt aacaatgatg gcgaccactt
    12421 tatcgctttg cccacgcata gcgcgcgcag agcgggaagg ggaaaatcca tgctgattca
    12481 tcactgcttc aacacgctcg cgcgtgcgct gactcacgcc gctttcgtta ttcagcaccc
    12541 gggaaaccgt agatttcccc acgccgctta agcgcgcgat gtctttgatg gtcagccgat
    12601 tttgcatcct gttgtcccat aacgtgttgt ttaattattt gagcctaact ttacccgtgc
    12661 attcagcaat gggtaaagtc tggtttatcg ttggtttagt tgccagcagg tattatatcg
    12721 ccataaatgc tacgaatatt attggattct ccttattatt tgcggcgctt ttttcactta
    12781 ccggaggtta tatggaacct gatcccacgc ctctccctcg acggagatta aaacttttcc
    12841 ggtaagcccg tcttttcacg gcgttaccgg atgcgtaagg ccgtgacgtt ttaatgtccc
    12901 tgctcagctt tattaccttc aggtaaggct tcgccacgcc tgaagacatt tctgtactgt
    12961 ttcagacagt gcggagggac tccttatgtt taaagaaatt tttacccggt tcattcgcca
    13021 tttaccttcc cgtctggttc atcgcgatcc attgcctggc gcgcagcaga cagtgaatac
    13081 ggcggtcccg ccgtccttaa gtgcgcattg cctgaaaatg gcggtgatgc ccgaagaaga
    13141 attgtggaaa acgttcgaca cccatccgga aggattaaat caggcggaag tggaatctgc
    13201 ccgcgaacaa catggtgata ataaattacc cgcccaacaa ccgtcgccgt ggtgggtaca
    13261 tttatgggtc tgctatcgca acccctttaa tattttgctc accattcttg gtgctatttc
    13321 ttacgccacg gaagatttat ttgccgcagg tgttatcgcg ctaatggtcg ctatttctac
    13381 gttgctgaac tttattcagg aagctcgttc caccaaagcg gcagatgccc tgaaagcgat
    13441 ggtcagtaat accgcgacgg tactgcgagt aattaacgac aaaggcgaaa atggctggct
    13501 ggagatcccg atagaccagc tggtgcccgg cgatattata aaactggcgg cgggggatat
    13561 gatcccggca gatttacgta tcttgcaggc gcgggatctg ttcgtcgctc aggcgtcgtt
    13621 aaccggtgag tctctgcccg tggaaaaagc cgctactact cgccagccgg agcacagcaa
    13681 tccgctggag tgcgacaccc tgtgttttat gggcaccacc gtggtgagcg gcacggcaca
    13741 agcaatggtg attgctacag gagccaacac ctggtttggt caactggcgg ggcgtgttag
    13801 tgagcaggaa agcgagccga atgcctttca gcaagggatc agccgcgtta gtatgctgct
    13861 gattcgcttt atgctggtga tggctccggt ggtgttgctt atcaacggtt acaccaaagg
    13921 cgactggtgg gaagcggcac tgtttgcgct ttcggtcgcg gtaggcttaa cgccggaaat
    13981 gttgccgatg attgttacct cgacgctggc gcgcggcgca gtaaagctgt cgaaacagaa
    14041 agtgatcgtc aaacatctgg atgccattca gaactttggc gcaatggata ttctgtgcac
    14101 tgataaaacc ggcaccctga cgcaggacaa aattgtgctg gagaatcata ccgatatctc
    14161 cggtaaaacc agcgaacgcg tgctgcatag cgcgtggttg aacagtcatt accagaccgg
    14221 acttaaaaat ctgcttgata ccgcggtgct cgaaggtacg gatgaagagt cagcgcgttc
    14281 gctggccagc cgttggcaaa aaattgatga gattccgttt gatttcgagc gtcgtcggat
    14341 gtcggtggta gtggcagaaa ataccgaaca ccatcagctg gtttgcaaag gtgcattgca
    14401 ggaaatcctc aatgtgtgtt cgcaggtgcg tcacaatggc gagattgtac cgctcgatga
    14461 caccatgctg cgtaagatta agcgggttac tgatacgctg aatcgtcagg ggctgcgcgt
    14521 ggttgcggtg gcgacgaaat acctgccagc gcgtgaagga gattaccagc gggcggatga
    14581 atccgacctg atcctcgaag gatatattgc tttccttgat ccgccaaaag agacaaccgc
    14641 tccggcactg aaggcattaa aagcgagtgg aattaccgta aaaatcctta ctggcgacag
    14701 tgagttagtt gcagcgaaag tgtgccatga agtggggctg gatgcgggag aggtggtcat
    14761 tggtagtgat attgaaacgc tatctgacga tgaactggca aatctcgcac agcgcaccac
    14821 gctgtttgcc cgcctgacgc cgatgcataa agaacgcatt gtgactttac tgaagcgcga
    14881 agggcatgtg gttggcttta tgggcgatgg tattaatgat gcgcccgcct tacgcgctgc
    14941 ggatatcggc atttctgtgg acggcgcggt agatattgcc cgtgaagcgg ctgatatcat
    15001 cctgctggaa aaaagcctga tggtgctgga agagggggtt attgagggac gtcgcacttt
    15061 cgccaacatg ctgaaataca tcaaattgac ggcgagttct aacttcggta atgtgttcag
    15121 cgtgctggtg gcgagtgctt tcttgccctt cctgccgatg ttgccgttac acttgcttat
    15181 tcagaacctg ctgtacgatg tgtcacaggt ggcgatcccg tttgataacg tcgacgacga
    15241 gcaaattcaa aagccgcagc gttggaatcc ggcggatctg gggcgcttta tggtcttctt
    15301 cggaccgatc agctcgattt tcgatattct gacgttttgc ttgatgtggt gggtattcca
    15361 tgccaacacg ccggaaacgc aaacgctgtt ccagtcgggc tggtttgtgg tgggattact
    15421 gtcgcaaacg ctgattgtgc atatgatccg tacccgccgt gtgccgttta ttcagagctg
    15481 tgcatcgtgg ccgttaatga tcatgaccgt gatcgtgatg attgtcggga tcgcattgcc
    15541 gttttcaccg ctggccagtt atctgcagct gcaggcgctg ccgttaagct atttcccgtg
    15601 gctggttgcg attctggcag ggtatatgac gttaacccag ttggtgaaag ggttctatag
    15661 ccgtcgttac ggctggcagt aaagaataaa ctgggcatgg tggcccggat tcagattgca
    15721 gacaaagtga gctttgttca tgccggatgc ggcgtaaacg cccaatccgg cctacaaatt
    15781 caataaattg caggaatcac gtaggcctga taagcgtagc gcatcgggcg attttgcttt
    15841 tgtcatcaga cttaatccgg gcatgatagc ccggatttcc atcaagatta gcgacgaaca
    15901 gcgattgctt cgatctcaat cttcacgtct ttcggcagac gggcaacttc aacgcaagaa
    15961 cgtgccggga aagtggcgtt gtgttcggtg aagaaggctt cgtaagtggc gtttacggtt
    16021 gcgaagtcgt tcagatcttt cacgaacaca gtggttttaa cgatgtcgcc cactttcagg
    16081 ccagcggctt cgacgattgc ttttacgtta tccagcgact gacgtgcctg tgcagcgacg
    16141 tctgccggta cttcaccagt tttcggattt accgggatct gaccggaggt gatgatcata
    16201 ttgcccagat caacgccctg tacgtaagga ccgatagctg ccggtgcatt ttccgtcgcg
    16261 atagttttgc tcatgatttc tcctttatta cagcggtaaa aagtctggct cattataggg
    16321 agccagactt ttattaccaa ccgcaattaa ttggccagca ccacattatg ggaaaactct
    16381 ttttcacagt atttgcattt aagcgcgatg tcattggcgc gttttcgcac ggcaaagctg
    16441 gatgaaaccg gttcggcatg gctgatacag ttgctgttcg ggcagaccag cacattgtcg
    16501 atgcgctctg gcagacttgg gcgcgattta cccaccactt catagttgtc gatacggtta
    16561 accgtggctt gcggtgcata caatgccagt tggtctactt gctcttcact caaaaaggta
    16621 ttttcgattt tgatcagatc tttgcggccc atctcgccag aaggcaaatt cagaccaatg
    16681 gtgatgcgct gatctgtttc ggtcaacttg aacagactca acagcttaaa accgatctgg
    16741 gcggggatat ggtcaattac cgtgccgcgt ttaatagctt caacctgcaa tttattatcg
    16801 tgtgtcatct ctatttcccc ttacagtacc agatcgcgat tcagaaccag tgccagtaac
    16861 gcctggcgag cgaaaatccc gttgcctgct tgctggaagt accaggcgtg aggcgtttta
    16921 tccacatccg tcgcaatctc atcaacacgc ggcagcggat gcagcacttt catattggct
    16981 ttggcgttgt ggagatcgct ggcgcgaaga acaaactgcg ctttcacgtt ggcgtactcg
    17041 gacgggtcca gacgctcttt ttgcacgcgg gtcatgtaca ggatgtctac ttccgccatc
    17101 acttcttcaa tagagctgtg cagactccag gcgatccctt tttcatcgag catatccaga
    17161 atgtattgcg gcattgccag cgcatccggc gcgataaagt aaaaacggtt gccgtcgaac
    17221 ttcgctagcg cctgggtcag ggagtgaacg gtgcggccat atttcaggtc accaaccatt
    17281 gcgacgtgga gattgtccag acgcccctgg gtttcctgaa tggtgaataa gtccagcaag
    17341 gtttgcgtcg gatgttggtt ggagccatca ccggcattca gtaccggtac attgccagaa
    17401 aattcggtgg ctaggcgcgc cgcaccttcc tgcggatgac gcatcactat cgcatcgacg
    17461 taagtgctga taaccgaaat ggtatcggcc agcgtttcgc cttttttacc cagtgatgta
    17521 ttggcgctgt cggagaagcc caccacgctg gcacccaggc ggtgcatgga agtttcgaaa
    17581 gagaggcggg tacgggtaga ggcttcgaag aaacagctgg caatgacttt gtgcttcaac
    17641 agctctggtt gcgggtttgc tttcagtttc gccgctgtcg ccagcaccag attaaggtca
    17701 tcgcgactaa ggtcgtttat ggaaatgata tgtttctgat atagcggatt agccatcttt
    17761 tatctcctga cacctaggca aaaaaaagcc cctcgattga ggggctggga atgggtgatc
    17821 aacgggaaga aaaacggcag gccagcgtct tttttcagac gcggtaagac aaaatgtcga
    17881 acacactgaa ccatacatcc tcccggcaaa ttgtccggca ttatactcat agccagaagc
    17941 ggtgcaagca tttgatgcaa tattttgtca gcacaaacgg tttatttgaa ttaaaagtca
    18001 aaatatatgc atttttattt tctatgattc tggaggggaa gtgtcgttcg gtatcaataa
    18061 ttgcagacat ttcctgcttt tttctttttc attttcacgc aatcagaaat gcattttatt
    18121 ccattcatcc gacttatagg gcggagggtg aaaacgaacg gctaacacta ttgcttatag
    18181 ctcagggata cgcgctatca ctttaatttc aaaatcaaag cctgccagcc aggtaacacc
    18241 caccgccgtc cagtttggat aaggtggggc gctaaatatt tcatttttca ccgtcatgat
    18301 gtcttcaaat tgtttttccg gatcggtatg aaagctcgta acatcaatga tatcgtcaaa
    18361 agtgcatccc gcagctgcca gggtcgcatg caaattatca aaggccagtc tgacttgttg
    18421 ctgaaaatcg ggttctggtg ttccgtcctc tcgacttcct acttgcccgg aaacaaacag
    18481 caaatcgccg gaacgaatag ccgcagaata acgatgctca gcatacagtg aatgtcggcc
    18541 agcagggaaa acagcggttc tttctaccat ttggttatcc tcaagattta cgacatgaac
    18601 agaagattta tatttatcgg gagccgcttt tagggggacg acgttagtaa acaaaaccca
    18661 gacatcatgg ataatggctg ggcttaattg agcgtagtcg gttatgcgcc aaacgcgcca
    18721 tcaatggtat gcatcgcgcc ggtaacaaaa ctggcttctg gccctgctaa ccatgcgacc
    18781 ataccagcga cctcttccgg ttgcccatgt cttttgatgg ccataaaact atgcaacata
    18841 tcgcgcattg gcccgttggc gggattaacg tcggtatcaa ttggtcctgg ctggacgacg
    18901 ttgatggtga tccctcgcgg tccaaaatca cgggccagcc cgcgcgccat gccttgcagg
    18961 gcagatttac tggcggcata agcagccatg cctgcaacag gcatacgatc gccattcacg
    19021 gagccgatga ttaagatgcg cccgccttcg ggcatctgcc gggcggcttc aacagaggca
    19081 tgataaggag catgaatatt gattttgaaa aggcgatcaa tatcgtcggc atttaattcc
    19141 agggcatcgc caaagacgcc aatacctgca tttaccacca ggatatccaa tgcgccgctc
    19201 ttacgaacga catcaataac agcgtctctg tcagcactat ctgtgaatac tgctgtcgct
    19261 ccagtctctt gtgccagacg ttcagcggca tctttcgacc ccgcataggt gaatcgtaca
    19321 ttggccccat cggtgacgaa acgacgtacg atagcggcac cgattccacg actgccaccg
    19381 aggatgagaa ctgtcttacc ggtaaaagcg cccataagga ctccttgatt tattatgtaa
    19441 catgcattac aaaactgttt taactttctg tcaacatgtt ttgtaatggt cactaaaaaa
    19501 caatctcgcg ttccaggtcg tcccagacgt ttcgctcctg agcaggcagt ctctgcggca
    19561 aaagtgcttt ttcaccaaaa aggtttcgat gcagtaagtg tctctgaagt tactgattat
    19621 cttggtatta accccccgag tctctacgcg gcttttggca gtaaggctgg gttatttagc
    19681 cgtgtactca atgaatatgt cggtacggaa gctattccgc ttgccgatat tcttcgtgat
    19741 gatcgtccgg taggcgagtg cctggttgag gtattaaaag aagcggcgcg cagatatagc
    19801 caaaacggcg gctgcgctgg ctgtatggtt cttgaaggta ttcatagtca tgatccacaa
    19861 gcgcgtgata ttgccattca atattatcac gccgcagaaa cgaccattta tgactatatc
    19921 gccaggcggc atccacaaag cgcacaatgt gtgactgatt ttatgagtac cgtgatgtca
    19981 gggctttctg cgaaggcacg ggaggggcac tccatcgaac aactctgtgc aacagctgcc
    20041 ctggcagggg aagcgataaa aactcttctc aaggagtgat gctggccttg atccgaaagg
    20101 cgggaacgcg cctgccgata agttgtgata agacaataat tcacgcgtta aggctggcgg
    20161 aattgatcat cttttcgtat aacgatagaa atgaaacatt attttaatta aggagtggaa
    20221 aagatgatca tcggaaatat tcataatctt cagccgtggc taccccagga gttacgccag
    20281 gcgattgagc atatcaaagc acacgttacg gcagaaacgc caaagggcaa gcatgatatc
    20341 gaaggcaatc gtctgtttta tcttatctcg gaagatatga ccgagccgta cgaagctcgc
    20401 cgtgcggagt accatgcccg ctatcttgac attcagattg tgttaaaagg tcaggaaggc
    20461 atgaccttca gcacgcaacc tgcaggcgcg ccggataccg actggttagc tgataaagac
    20521 atcgcatttt tgccggaagg cgttgatgag aaaacagtta tcctaaatga aggtgatttt
    20581 gttgtgtttt atccggggga agtgcataaa ccgctgtgcg cagtgggcgc gccagcccag
    20641 gttcgcaaag cagtagtgaa gatgctgatg gcgtgatgac ttttcgccgt aaataactcc
    20701 aggtttacgg cgagtttgtg aaaagagcgt tttttgatat ttttttgtga gtaaaatttg
    20761 taatgcttag acgttcttat taactcaagg agttcgtcat gggcaaaata tcagatttga
    20821 attattctca acacattaca ttagccgaca attttaaaca aaaaaatgaa gccttagata
    20881 cctggtatgt gggaatgaat gattttgccc gaattgccgg ggggcagaat agcagaagca
    20941 atattctttc tcctagagca tttttggagt ttttggcaaa gatatttacc ctgggttatg
    21001 tggattttag caaacgctcc aacgaagcgg gtagaaatat gatggctcat attgagttct
    21061 catcttattc taaagatact gatggcaatg aaaaaatgaa gttttacatg aataatcctg
    21121 aaggggagcg agcggattta tctaaggtga agatagaaat tacacttgcc tcagcttcta
    21181 ctaaagggat tcgtgaagga catacagtca ttatatttaa acaatctgat ggttcgacta
    21241 atcgttatga agggaagtct tttgaaagaa aagatgatag ttcattacac ctgattacta
    21301 acaaggttct ggcgtgttac caacgcgaag ctaacaagaa aatagcgcgt ctattaaata
    21361 atcatcagaa gttaaataat atacaggagt taaatgattc gcaggagtta aataattcac
    21421 agaagttaaa taattcgcag gagttaaata actcgcaggt gagttgtaaa ggttcagttg
    21481 attctacgat tacggattta ttagaaaaag cattgaata
//
LOCUS       DAJTRB010000069        21489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTRB010000069 DAJTRB010000000
VERSION     DAJTRB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.069"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(42..2111)
                     /gene="glyS"
                     /locus_tag="OO330_002769"
     CDS             complement(42..2111)
                     /gene="glyS"
                     /locus_tag="OO330_002769"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5883575.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGIIV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2121..3032)
                     /gene="glyQ"
                     /locus_tag="OO330_002770"
     CDS             complement(2121..3032)
                     /gene="glyQ"
                     /locus_tag="OO330_002770"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5883576.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3127..3426)
                     /locus_tag="OO330_002771"
     CDS             complement(3127..3426)
                     /locus_tag="OO330_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5883577.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3601..4596
                     /gene="wecH"
                     /locus_tag="OO330_002772"
     CDS             3601..4596
                     /gene="wecH"
                     /locus_tag="OO330_002772"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5883578.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4638..5075)
                     /locus_tag="OO330_002773"
     CDS             complement(4638..5075)
                     /locus_tag="OO330_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5883579.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5121..5462)
                     /locus_tag="OO330_002774"
     CDS             complement(5121..5462)
                     /locus_tag="OO330_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883580.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5631..7085)
                     /gene="xylB"
                     /locus_tag="OO330_002775"
     CDS             complement(5631..7085)
                     /gene="xylB"
                     /locus_tag="OO330_002775"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5883581.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7157..8479)
                     /gene="xylA"
                     /locus_tag="OO330_002776"
                     /pseudo
     CDS             complement(7157..8479)
                     /gene="xylA"
                     /locus_tag="OO330_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8845..9837
                     /gene="xylF"
                     /locus_tag="OO330_002777"
                     /pseudo
     CDS             8845..9837
                     /gene="xylF"
                     /locus_tag="OO330_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
     gene            9915..11456
                     /gene="xylG"
                     /locus_tag="OO330_002778"
     CDS             9915..11456
                     /gene="xylG"
                     /locus_tag="OO330_002778"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5883582.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11434..12615
                     /gene="xylH"
                     /locus_tag="OO330_002779"
     CDS             11434..12615
                     /gene="xylH"
                     /locus_tag="OO330_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5883583.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSTRNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12693..13223
                     /locus_tag="OO330_002780"
     CDS             12693..13223
                     /locus_tag="OO330_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5883584.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13335..13871
                     /locus_tag="OO330_002781"
     CDS             13335..13871
                     /locus_tag="OO330_002781"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5883585.1"
                     /translation="MIGIDNEELTRYLSRIALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPDVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13979..14803)
                     /locus_tag="OO330_002782"
     CDS             complement(13979..14803)
                     /locus_tag="OO330_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5883586.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15123..17153
                     /gene="malS"
                     /locus_tag="OO330_002783"
     CDS             15123..17153
                     /gene="malS"
                     /locus_tag="OO330_002783"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5883587.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17331..18566
                     /gene="avtA"
                     /locus_tag="OO330_002784"
     CDS             17331..18566
                     /gene="avtA"
                     /locus_tag="OO330_002784"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5883588.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDDALANQHGIPLVIDNAYGVP
                     FPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGIISLAPG
                     GIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHKPEGAIF
                     LWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYVPEPEKI
                     EAGVKILAEEIERAWAESH"
     gene            complement(18717..19190)
                     /locus_tag="OO330_002785"
     CDS             complement(18717..19190)
                     /locus_tag="OO330_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5883589.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19292..20140)
                     /gene="yiaJ"
                     /locus_tag="OO330_002786"
     CDS             complement(19292..20140)
                     /gene="yiaJ"
                     /locus_tag="OO330_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5883590.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20341..21333
                     /gene="yiaK"
                     /locus_tag="OO330_002787"
     CDS             20341..21333
                     /gene="yiaK"
                     /locus_tag="OO330_002787"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5883591.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21345..>21489
                     /locus_tag="OO330_002788"
     CDS             21345..>21489
                     /locus_tag="OO330_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5883592.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5406 a         5485 c         5271 g         5327 t
ORIGIN      
        1 aaaaagcctg ccagatggca ggctttttaa taacggcgtt attactgcaa cagcgaaata
       61 tccgcaacgc gcaggaacag ttcgcgcagt ttctccagca tggtcagacg gttgagacgc
      121 aattctttgt catcaaccat gaccatcact ttatcgaaga aagcatcaac cggctcacgc
      181 agctctgcca gttcgaccag cgcatcctgg taacgacctt ccgcaaagta cggctccagc
      241 ttgtcacgta gcaccacaac ctgcatcgcc agtttaattt cttccggctc tttcagggta
      301 gaggcattca cgcgatcgct cagcacttcg tcagatttcg ccaggatgtt agatacacgt
      361 ttgttcgccg ccgccagtgc tgcagctgct tccagggtac ggaagtgcga taccgctttc
      421 atgcgggcat cgaaatcagc cggacgagtc ggacgacgcg ccagtaccgc ctggatggtg
      481 tcaacggtat aaccttcgtc ctgataccag gcgcggaagc gaccgagcat aaagtcgata
      541 acatcatcaa ctacgttggc attagtcagc ttatcgccat acagacgcac cgcttcttcg
      601 gtcagcgttt gcagatcaag gttgaggttc ttctcaacga taattcgcag cacgccaagt
      661 gcggcacgac gcagcgcaaa cgggtctttg tcgcctttcg gatgctgacc gataccgaag
      721 atacccgcca gagtatccat cttatcagca atcgccagcg cacaagctac cgggttggac
      781 ggcaggtcat caccggcaaa gcgcggctga tactgctcat tcagcgcgac ggctacgtct
      841 tccgcttcgc catcgtgacg cgcgtagtgc atccccataa cgccttgggt gtcggtgaac
      901 tcgaagacca tgttggtcat caggtcacac ttagacagca ggcccgcacg ggttgcgtgg
      961 ttaacgtcag cgccaatctg ttcggcgatc cagccagcca gcgcctggat gcggtcagtc
     1021 ttgtcacgca gcgtacccag ctgttgctgg aacagcacgg tttgcaggcg cggcaggtta
     1081 tcttccagac gttttttgcg gtcggtgttg aagaagaact cggcatccgc cagacgtgga
     1141 cgaacgactt tctcgttacc agagataatt tgctgcggat ctttcgattc gatgttggca
     1201 acgaagataa agttcggcag cagtttgccg tcgttcgcat acaccgggaa gtacttctgg
     1261 tcacctttca tggtgtaaac cagggcttca gccggcaccg cgaggaattt ctcttcgaat
     1321 tttgcggtca gaacgaccgg ccactccacc agcgaagcca cttcttccag caggctttcg
     1381 cttaagtcag cgttaccgcc aatcttacgc gctgcttctt cggcatcggc tttaatcttc
     1441 gctttacgtt cttcgtaatc ggcgatgact ttcccgcgct cacgcagaat ttccggatac
     1501 tgatcggcgt tgtcgatggt gaattccggt tcgcccataa agcggtggcc gcgaatcacg
     1561 cgatcggact gaatgcccag aatggttgcc ggaatgactt tgtcgcccag cagcagggtc
     1621 acggtgtgca ccggacgcac gaagtgcacg tcgcttgcac cccagcgcat cagtttcggg
     1681 atcggcagtt tcgccagcga agtcgcaacc atattcggca gcagtgcttc ggtgctttcg
     1741 cccttcacat gggcgcgata cagcagccat tcgcctttat cggtagtcag acgctcagcc
     1801 tggtcaacga taataccaca accacgcgcc caaccttctg ccgctttgct cggttttcct
     1861 tcagcgtcga acgcctgggc aatcgccggg ccgcgttttt cgatttcacg atctggttgt
     1921 gcttccgcca ggttagctac tttcagcgcc agacgacgcg gagcagcaaa ccattgaacg
     1981 gtgccgtgtg cgaggcctgc gttatccagc tccgcagtaa agttcgcagc aaaggactct
     2041 gccaggctgc gcagtgcttt tggtggcagc tcttcagtgc cgatttccac cagaaaagtt
     2101 ttctcagaca tagccgcctc ttacttatct ttgttgcaca tcgggaagcc gagggcttca
     2161 cgggaagcgt aatatgcttc tgccactgct ttggtcaggg tgcgaatgcg cagaatgtag
     2221 cgctgacgct cggtgacgga gatggcttta cgcgcatcca gcaggttgaa gctgtgggcg
     2281 gctttcagaa tacgctcgta ggctggcagc ggcagcggat tttccagcgc cagcagctgc
     2341 tgcgcttctt tctcgtactg ctcaaagcag gtgaacagga agtccacatc cgcgtattcg
     2401 aagttgtaag tggactgctc cacttcgttc tgatggaaca cgtcgccgta ggtggtttta
     2461 cccagcgggc cgtcgctcca gactaggtcg taaacgctgt ctacgccctg aatgtacatg
     2521 gccagacgtt ccagaccgta ggtgatctcg ccggtaaccg gtttacactc cagaccacca
     2581 acctgctgga agtaagtgaa ctgcgtcact tccatgccgt ttagccacac ttcccagccc
     2641 agtccccagg cccccagagt cgggttttcc cagttatctt ccacgaaacg gatgtcgtga
     2701 atagtcgggt ccatgcccag ctctttcaga gaaccgaggt acagctcctg aatattgtcc
     2761 ggcgatggct taatgaccac ctggaactga tagtagtgct gtaaacggtt ggggttttcg
     2821 ccgtagcgac catcggtcgg gcgacgagaa ggctgaacat aagcagccgc catcggttct
     2881 ggccccagcg cgcgcagaca ggtcattggg tgagaggttc ccgcgccgac ttccatgtcc
     2941 aatggttgaa caatggtgca gccctggcga gcccagtaat cctgtaaggt caggatcaag
     3001 ccctggaagg tcctggtatc aaacttttgc atattatttc gtgctggata cgtgtggatt
     3061 taaaggaagg gatcagtata cccgctggat ggaagatata cagtacgaaa cgggaaaaag
     3121 cagggcttaa cgcatggaaa gatgcaaaaa atggccatct gcgtcaaaag aacagacaaa
     3181 ctgctcttta cggaaggtat aaccgcgcat ttcatagctt ccctggaact gttcgaaggc
     3241 ggtgacgtcg attttttgct cgccggtgtt ataacgtttt gccgcctgat ccttgcataa
     3301 cgcttccata ttcaacgaac gcagaggatc aacctttacc cgttgtgctt tctgcacggg
     3361 tgacggtgta gaacaaccca ctagcaccat aagcgccatt gccggaaaga aagcgttcat
     3421 catcattaaa ttaccgtctg gtttgcaggt gagtcttatt atttatatcg gtatagtggc
     3481 tgatatctta cgaatttcct taagcctcta taggctgtag tgataaaact cagattattc
     3541 cagtgcgcca accaaatgat ttatcggtca gacttttttt aaccagatac agagacacag
     3601 atgcagccca aaatttactg gattgataac ctgcgaggga tagcgtgttt aatggtggtg
     3661 atgattcaca ccactacctg gtatgtgacc aatgctcata gtgttagccc cgtcacatgg
     3721 gatatcgcca atgttctgaa ttctgcctct cgtgtcagcg tgccgctatt tttcatgatt
     3781 tccggctatc tcttttttgg cgaacgcagc gcccagccgc gccatttctt gcgtatcggc
     3841 ttatgtctga ttttttatag cgcaatcgca ctgctctaca ttgcgctgtt tacctccatc
     3901 aatatggagt tagcgctgaa aaacctgctg caaaagccag tgttttacca cttgtggttt
     3961 ttcttcgcga ttgcggtgat ttatctggtt tcaccgctga ttcaggtgaa gaacgtcggc
     4021 ggaaaaatgt tgctggtgct aatggtggtg attggcatta tcgctaaccc aaacacagtg
     4081 ccgcagaaaa ttgacggttt tgaatggctg ccaattaact tatatatcaa tggcgatact
     4141 ttttactaca ttctgtatgg catgttgggc cgcgctatag ggatgatgga cacacagcat
     4201 aaagcactgt cgtgggtgag cgccgcgctg tttgcgacgg gggtttttat tatctctcgc
     4261 gggacattat atgaattgca gtggcgcgga aattttgccg atacctggta tctttactgt
     4321 gggccgatgg tttttatctg cgcaatcgcg ctattgactc tggttaaaaa cacgctggat
     4381 acgcgtacca ttcgcggact tggcttaatc tcccgccatt cattgggtat atacggattc
     4441 cacgccttga ttatccatgc gctgcgcacc cggggaattg agcttaaaaa ttggccaata
     4501 ctggatatta tttggatctt ttgcgcgacg ttggcagcga gtttgttact ttctatgctg
     4561 gtacaacgaa tcgacagaaa cagattagtg agttaagtaa aagcccgttc acattggact
     4621 gaccgggctt acgtgaatta ttcgctgtat tcttcctggg taacctgtgt ttcttttggc
     4681 gggtttattc cggcatcacg aatattcttc tgcaccgcta ctgcaccaaa caagcttaag
     4741 aagaaagcca gtccataaaa acctttttca ctgagtaata atgtcgcatt ccacagacct
     4801 accatcagta gagcaacaga gataataaag acagtcaggc aggtcatata ataaatggaa
     4861 gtggtcggta tgccttcata cttgtcccga acggtctttt gataagacgc agcagaaaac
     4921 agtcctaaca ccagtacggc aaaataatac cctttttcat ttaactgcat ctctgcattc
     4981 catagcccta acaggtaggt aacgatacca ccaacgagag ctatccatga cacaatacta
     5041 aaggccggtg aataggttga tattttgttg tccatcacag tattcctttc attcctgaat
     5101 gtgtaagagc tttatgttgc tcataccgag cgaaacaaac gcatttgacc aaacaagcag
     5161 acaaaaaagg cgatggggta gatcaccatt tcataagcat tgataggtgc atttaaaaca
     5221 ccgataaaca agagaccgat agtgattaac gcaactaact ggcagatatg ggtaaaaaaa
     5281 ttatccagtt gcccgagttt ttctgcgcga agatatgcat agttaccaaa agttgctgtc
     5341 attaacacgc caagaaaata gccttttcca cttagcaatg ggcatgagat ccatagccca
     5401 accagataaa ccagcgcccc gacaacaaat aatagttttg ccacagtttt tgaggttttc
     5461 ataacgatct ccatatctac cagcgataca ttacgagtaa ccaacgaaag acaaaactga
     5521 aaaatgccat taacaaatga ttttcagaat aaattcatac taaatattaa ttaattagtg
     5581 agatatatag atgtgaatta tcccccaccc ggtcaggcag gggataacgt ttacgccatt
     5641 aatggcagaa gttgctgata gaggcgacgg aacgtttcac gtcgtggctg ataagcggca
     5701 taacgctgcg catctggtag atgcgactgt tctaacggta gctgcggcaa caactcaatg
     5761 agcgatttct ctggattcgc cgcaatctgc gccagccttg ctgcgcccag tgctggcccc
     5821 acatcccccc ccgtacggta atcgagctgc tgaccgctga tatccgccag catctgacgc
     5881 cagtactcac tacgcgcccc gccaccaatc aacgtaacac tttgcggttt aatgccgcag
     5941 gcatgcacga catccatgcc atctgccagc gcataaccca cgccttccag cactgctcgc
     6001 gccagttcat tggggccatg ttgatgagtc aaaccaaaga aaaccccctt cgcctgggga
     6061 ttattgtgtg gtgtacgctc gccggaaaga taaggcagaa accaaactgg ctcggcactt
     6121 tcatcagcct gttgagctgc agcgattaaa gctgggacat tgcacaggcc ggttaatttc
     6181 gcggcccaat ccagacacga tgctgcactc agcattacag acattaaatg ccaacgctgc
     6241 ggtagcgcat ggcaaaagct gtgtacagcg ctttctggct tgcttaagaa cccttcgctg
     6301 acagcaaaat agacccccga cgtccccagc gataacattg cctgattagc atcaaccatt
     6361 cccacaccaa ctgcaccagc tgcattgtcg ccaccgcctg cgacaactgg caccgtcgcc
     6421 ataccccacg ctttcgcaac ttcaggtaac aaagcaccag taatttcgct gccttcgtat
     6481 aatgcgggca tctggtcacg agataagtcg caagcctgca gcatgacgtc actccagtca
     6541 cgctttgcga catccagcca catggtgcca gctgcgtcag acatatcgct ggcaaactcc
     6601 cccgtcatac gcagacgcaa gtaatctttc ggtaataata ctttgtcgat ttgacggaat
     6661 atctccggct catgccgctg aacccatagc aatttaggcg cagtaaatcc gggcatcatc
     6721 aggttgccgg taatcactcg tgattgcgga actctcgctt ccagcaaagt gcactcttgc
     6781 gcacagcgcc cgtcgttcca caaaatggca gggcgcaata cccgttgttg agcatccagt
     6841 aaggttgctc catgcatctg gccggcaata cccaatgctt taacgtcctg cagagaatgc
     6901 tgatcgccca gagctttcat tgcgcgatca gttgcctgcc accactgttc cgggtcttgt
     6961 tccgaccaaa gtggatgcgg gcgcgaaacg gtcagctttt ccgtttgcga agcaaccacc
     7021 tcaccctgct cgttgagcaa aataactttt acgcccgagg tgccaagatc tatcccgata
     7081 tacatatcga tcgttcctta aaaaaatgcc cggtatcgct accgataacc gggccaacgg
     7141 actgcacagt tagccgttat ttgtcgaaca gataatgatt taccagattt tccagttgct
     7201 cctggcgacc actctgatgc accggagaca aattatgttc ctgagcatat ttggctaaat
     7261 ctgccagtga catttggcct ttcaggattt gctggcccaa ttcgctattc cagccggaat
     7321 aacgctgcgc gatgcgttta tccagctcgc catcttcaat catgcgcgct gcaattttca
     7381 gcgccagtgc catcgtatcc atcgcgccga tatgaccgta aaacagatca tatttatcag
     7441 tactttgacg acgtactttg gcatcgaagt tcagaccacc ggtggtgaaa ccgcctgctt
     7501 tgagaatttc atacatcacc agcgcattct cttccacact gttcgggaac tggtcggtgt
     7561 cccagcccag ttgcgcatcg ccacggttgg cgtcgacaga accgaacagg ccaagcgcaa
     7621 tggcggtggc tatttcatga tggaaagagt gacctgccag cgtcgcgtgg ttagcttcaa
     7681 tgttcagttt aatctctttt tccagaccaa actgtttcag gaagccatag accgtcgcgg
     7741 catcgtaatc atattgatgt ttagtcggtt cttgcggttt cggttcgata agcaacgtgc
     7801 cctagaagcc gattttatgt ttatgctcaa ccaccatctg cataaagcgg cccagttgtt
     7861 cacgctcctg acgcaagtcg gtatttaaca gcgtttcgta accttcacga ccaccccaca
     7921 ggacatagtt ttcaccgccc aatttatggg ttgcttccat cgctgtaaca acttgcgttg
     7981 ccgcccagct gaagacttca ggatctgggt tcgtcgccgc acccgcgcca tagcgagggt
     8041 ttgtaaagca gttagcggtt ccccacagca gcttcacgcc gctctcttct tgcttggctg
     8101 ccaggacatc aaccatttgc gcaaaattat tgatgtactc ttttaacgac gcgccctcag
     8161 gggaaacatc cacatcgtgg aagcaataaa atggcacatg taacttgtgg aaaaactcaa
     8221 atgcgacatc tgctttacgc ttcgccaacg ccagtgcctc accaggctgc tgccacggac
     8281 gattaaacgc ccccacacca aacatgtccg ccccgttcca gcagaaggtg tgccagtagc
     8341 aggcggcaaa acgcaagtgc tcttccatac gcttacccaa caccagttcg tcgggattgt
     8401 agtgacggaa tgctaacggg tttgaggatt ttgagccttc ataacgaacg cgatcgagct
     8461 ggtcaaaata ggcttgcata ttgaactcca taatcaggta atgccgcggg tgatggatga
     8521 tgtcgtaata ttgggcactc cctttcaatt gctcaattat gttatttcac actgctattg
     8581 agataattca caagtgtgcg ctcgctcgca aaataaaatg ggatgatgaa actgggtaat
     8641 tcctcgaaga taaaaatgca ataagtacaa ttgcgcaaca aaagtaagat ctcggtcata
     8701 aatcaagaaa taaaccaaaa atcgtaatcg aaagataaaa atctgtaatt gttttcccct
     8761 gtttagttgc taaaaattgg ttacgtttat cgcggtgatt gttacttatt aaagctgtcc
     8821 tctaactaca gaaggcccta caccatgaaa ataaagaaca ttctactcac cctttgcacc
     8881 tcacttctgc ttaccaacgt tgcggcacac gccaaagaag tcaaaatagg tatggcgatt
     8941 gatgatctcc gtcttgaacg ctggcaaaaa gatcgagata tttttgtgaa aaaggcagaa
     9001 tctctcggtg cgaaagtatt tgtacagtct gcaaatggca atgaagaaac acaaatgtcg
     9061 cagattgaaa acatgattaa ccggggtgtc gatgttcttg tcattattcc gtataacggt
     9121 caggtattaa gtaacgttgt aaaaaaagcc aaacaagaag gtattaaagt attagcttac
     9181 gaccgtatga ttaacgatgc ggatatcgat ttttatattt ctttcgataa cgaaaaagtc
     9241 ggtgaactgc aggcaaaagc cttggtcgat attgttccgc aaggtaatta cttcctgatg
     9301 ggcggctcgc cggtagataa caacgccaag ctgttccgcg ccggacaaat gaaagtgtta
     9361 aaaccttacg ttgattctgg aaaaattaaa gtcgttggtg accaatgagt tgatggctgg
     9421 ttaccggaaa acgcattgaa aattatggaa aacgcgctaa ccgccaataa taacaaaatt
     9481 gatgctgtag ttgcctcaaa cgatgccacc gcaggagggg caattcaggc attaagcgcg
     9541 caaggtttat cagggaaagt agcaatttcc ggctaggatg cggatctcgc aggtattaaa
     9601 cgtattgctg ccggtacgca aactatgacg gtgtataaac ctattacatt gctggcaaat
     9661 actgccgcag aaattgccgt tgaattgggc aatggtcagg agccaaaagc agatacctca
     9721 ctgaataatg gcctgaaaga tgtcccctcc cgcctcctga caccgatcga tgtgaataaa
     9781 aacaacatca aagatacggt aattaaagac ggattccaca aagagagcga gctgtaagcg
     9841 ttacgccccg gcgcggagcg ggggcgtgat ttctctccat gccgcgtgaa tgaattggct
     9901 taggtggagt cgttatgcct tatctacttg aaatgaagaa cattaccaaa accttcggca
     9961 gtgtgaaggc gattgataac gtcagcttgc ggttgaatgc tggcgaaatc gtctcactct
    10021 gtggggaaaa tgggtctggt aaatcaacgc tgatgaaagt gctgtgtggt atttatcccc
    10081 atggctccta cgaaggcgaa attatttttg cgggagaaga gattcaggcg agtcacatcc
    10141 gcgataccga acgcaaaggt atcgccatca ttcaccagga attggccctg gtgaaagaat
    10201 tgaccgtgct ggaaaatatc ttcctgggta acgaaataac ccacaatggc attatggatt
    10261 atgacctgat gacgctacgc tatcagaagc tgctcgcaca ggtcagttta tccatttcac
    10321 ctgatacccg cgttggcgat ttagggcttg ggcaacaaca actggttgaa attgccaagg
    10381 cacttaataa acaggtgcgc ttgttaattc tcgatgaacc gacagcctca ttaactgagc
    10441 aggaaacgtc ggttttactg gatattattc gcgatctaca acagcacggt atcgcctgta
    10501 tttatatttc gcacaaactc aacgaagtca aagcgatttc cgatacgatt tgcgttattc
    10561 gcgacggaca gcacattggt acgcgtgatg ctgccggaat gagtgaagac gatattatca
    10621 ccatgatggt cgggcgagag ttaaccgcga tttaccctaa tgaaccacat accaccggag
    10681 atgaaatatt acgtattgaa catctgacgg catggcatcc ggtcaatcgt catattaaac
    10741 gagttaatga tgtctcgttt tccctgaaac gtggcgaaat actgggtatt gccggactcg
    10801 ttggtgccgg acgtaccgag accattcagt gcctgtttgg cgtgtggccc ggacaatggg
    10861 aaggaaaaat ttatattgat ggcaaacagg tagatattcg taactgtcag caagccatcg
    10921 cccaggggat tgcgatggta cccgaagaca gaaagcgcga cggcatcgtt ccggtaatgg
    10981 cggttggtaa aaatattacc ctcgccgcac tcaataaatt taccggtggc attagccagc
    11041 ttgatgacgc ggcagagcaa aaatgtattc tggaatcaat tcagcaactc aaagttaaaa
    11101 cgtcgtcccc cgaccttgct attggacgtt tgagcggcgg caatcagcaa aaagcgatcc
    11161 tcgcccgctg tctgttactt aacccgcgca tcctcattct tgatgaacct accaggggta
    11221 ttgatattgg cgcgaaatat gagatctaca aattaattaa ccaactcgtc cagcagggta
    11281 ttgccgttat tgtcatctct tccgaattac ctgaagtgct cggccttagc gatcgtgtac
    11341 tggtgatgca tgaagggaaa ctaaaagcca acctgataaa tcataacctg actcaggagc
    11401 aggtgatgga agccgcattg aggagcgaac atcatgtcga aaagcaatcc gtctgaagtg
    11461 aaattggccg taccgacatc cggtggcttt tctgggctga aatcactgaa tttgcaggtc
    11521 ttcgtgatga ttgcagctat catcgcaatc atgctgttct ttacctggac caccgatggt
    11581 gcctacttaa gcacccgtaa cgtctccaac ctgttacgcc agaccgcgat taccggtatc
    11641 ctcgcggtag gaatggtgtt cgtcataatt tctgctgaaa tcgacctttc cgtcggctca
    11701 atgatggggc tattaggtgg cgtcgcggcg atttgtgacg tctggttagg ctggcctttg
    11761 ccacttacca tcattgtgac gttggttctg ggactgcttc tcggtgcctg gaacggatgg
    11821 tgggtcgcgt accgcaaagt cccttcattt attgtcaccc tcgcgggcat gttggcattt
    11881 cgcggcatac tcattggcat caccaacggc acgactgtat cccccaccag cgccgcgatg
    11941 tcacaaattg ggcaaagcta tctccccgcc agtaccggct tcatcattgg cgcgcttggc
    12001 ttaatggctt ttgttggttg gcaatggcgc ggaagaatgc gccgtcaggc tttgggttta
    12061 cagtctccgg cctctaccgc agtagtcggt cgccaagctt taaccgctat catcgtatta
    12121 ggcgcaatct ggctgttgaa tgattaccgt ggcgttccca ctcctgttct gctgctgacg
    12181 ttgctgttac tcggcggaat gtttatggca acgcggacgg catttggacg acgcatttat
    12241 gccatcggcg gcaatctgga agcagcacgt ctctccggga ttaacgttga acgcaccaaa
    12301 cttgccgtgt tcgcgattaa cggattaatg gtagccatcg ccggattaat ccttagttct
    12361 cgacttggcg ctggttcacc ttctgcggga aatatcgccg aactggacgc aattgcagca
    12421 tgcgtgattg gcggcaccag cctggctggc ggtgtgggaa gcgttgccgg agcagtaatg
    12481 ggggcattta tcatggcttc actggataac ggcatgagta tgatggatgt accgaccttc
    12541 tggcagtata tcgttaaagg tgcgattctg ttgctggcag tatggatgga ctccgcaacc
    12601 aaacgccgtt cttgattttg ataaaaattt tctcaaagcc ggttacgtat taccggtttt
    12661 gagtttttgc atgattcagc aggaaaagaa ccatgtttac taaacgtcac cgcatcacat
    12721 tactgttcaa tgccaataaa gcctatgacc ggcaggtagt agaaggcgta ggggaatatt
    12781 tacaggcatc acaatcagaa tgggatattt tcattgaaga agatttccgc gcccgcattg
    12841 ataaaatcaa ggactggtta ggagatgtcg tcattgccga cttcgacgac aaacagatcg
    12901 agcaagcgct ggctgatgtc gacgtcccca ttgttggggt tggcggctcg tatcaccttg
    12961 cagaaagtta cccacccgtt cattacattg ccaccgataa ctatgcgctg gttgaaagcg
    13021 catttttgca tttaaaagag aaaggcgtta accgctttgc tttttatggt cttccggaat
    13081 caagcggcaa acgttgggcc actgaacgcg aatatgcatt tcgtcagctt gtcgccgaag
    13141 aaaagtatcg cggagtggtt tatcaggggt tagaaaccgc gccagagaac tggcaacacg
    13201 cgcaaaatcg gctggcagac tgactacaaa cgctgccacc gcaaaccggg attattgccg
    13261 ttactgacgc ccgagcgcgg catattctgc aagtatgtga acatctacat attcccgtac
    13321 cggaaaaatt atgcgtgatt ggcatcgata acgaagaact gacccgctat ctgtcgcgta
    13381 tcgccctttc ttcggtcgct cagggcgcgc ggcaaatggg ctatcaggcg gcaaaactgt
    13441 tgcatcgatt attagataaa gaagaaatgc cgctacagcg aattttggtc ccaccagttc
    13501 gcgtcattga acggcgctca acagattatc gctcgctgac cgatcccgac gttattcagg
    13561 ccatgcatta cattcgtaat cacgcctgta aagggattaa agtggatcag gtactggatg
    13621 cggtcgggat ctcgcgctcc aatcttgaga agcgttttaa agaagaggtg ggtgaaacca
    13681 tccatgcaat gattcatgcc gagaagctgg agaaagcgcg cagtctgctg atttcaacca
    13741 ccttgtcgat caatgagata tcgcaaatgt gcggttatcc atcgctgcaa tatttctact
    13801 ctgtttttaa aaaagcatat gacacaacgc caaaagagta tcgcgatgta aatagcgagg
    13861 tcatgttgta gcgatgatga gaattgtcgg cgtcacatca gggaactcag cacagccacc
    13921 tgatgcgagc aaaagcctta tccgaccgac aaatcagata aggctcgagg aaatgaaatt
    13981 acatatgcgc cgcgattaac cgttggttat cctggtacat tgcgaacagg tagttgttat
    14041 aactcttccc tttggtcgag tagcccttca gcttgtgaat catcgctgtg gcagtcactt
    14101 cctgatccgc tttacgcagc tgtgcacgcg atttacggaa cgaagagtaa gccgggtgcg
    14161 tattcaggtt agtgacatag gcgctcaccg attctttgac agaactaaac tgtgagtacc
    14221 ctttcacttt acccggcgca ttggtacaac gtcctttcat gcatttcatg ccgaacagat
    14281 tgttattgtt gcgcgccagc ttcgacgttc cccaaccgct ttctgctgca gccatcgtcg
    14341 ccaccatact ggtagggata atgtctacgc gttcaagcaa ggtattccac gggatttttc
    14401 gcgtattacc agaccacttc accttgtagc gtttggcgat gtctttcaga cgcgcacgct
    14461 cagcaggtga ccattggccc tgatactgtt ttgaaatcag ccagttacgt tctgcagtaa
    14521 tggctgcatt ttggctggta atgtaaggca ttacggtccg gagaaacgcc tttttccttg
    14581 gtgttccgga agggtatttt cgcaaatcag gaagtgaact actctttgca ctattgcgag
    14641 aatactcttg tttactgctt acctgtttat tactagcttt agttaagtgg gacttttgac
    14701 tcgctgttgt tgtgtgcgtc ttcgctaaca cctcactcga aaacaccaga gtgagtaaca
    14761 taagaatcat cgccccatat cgtcgtatgg gagtcaaaat catcaggtct cctggtcgga
    14821 tttaatcatt ccaacacctt atatttttca caaatttgag agttgaatct caaatcatat
    14881 caaaaatagc tgtcaagagc accccaagga atagtccaaa tctgaaacta tgtcacgagt
    14941 taacgattca gattggcgct aaatcgcaga aaatgtgggg gttatcgcaa aattcagccg
    15001 ttttttgcgc gcgatcgctc acccttgctt ctcatcctgt ggacttaccg ctcagggatg
    15061 agttttgtct ggcttatcgc tggcaaactg tctgaaatcg cagcaataag gactcatcca
    15121 ccatgaaact cgccgcctgt tttctgacac tccttcctgg cttcgccgtt gccgccagct
    15181 ggacttctcc ggggttccct gcctttagcg aacagggaac gggaacattt gtcagccacg
    15241 cgcagttgcc caaaggtacg cgtccactca cgctaaattt tgaccaacag tgctggcagc
    15301 ctgcagatgc gataaaactc aatcagatgc tttccctgca accttgtagc aacacgccgc
    15361 ctcaatggcg attgttcagg gacggcaaat atacgctgca aatagacacc cgctccggta
    15421 cgccaacatt gatgatttcc atccagaacg ccgccgaacc ggtagcaaac ctggtccgtg
    15481 aatgcccgaa atgggatgga ttaccgctca cgctggatgt cagcgccact ttcccggaag
    15541 gagccgccgt acgggattat tacagccaac aaattgcgat agtgaagaac ggtcaaataa
    15601 cgttacaacc cgctgctacc agcaacggtt tactcctgct ggaacgggca gaaactgaca
    15661 catccgcccc tttcgactgg cataacgcca cggtttactt tgtgctgaca gatcgtttcg
    15721 aaaacggcga tcccagtaat gaccagagtt acggacgtca taaagacggt atggcggaaa
    15781 ttggcacttt tcacggcggc gatttacgcg gcctgaccaa caaactggat tacctccagc
    15841 agttgggcgt taatgcttta tggataagcg ccccatttga gcaaattcac ggctgggtcg
    15901 gcggcggtac aaaaggcgat ttcccgcatt atgcctacca cggttattac acacaggact
    15961 ggacgaatct tgatgccaat atgggcaacg aagccgatct acggacgctg gttgatagcg
    16021 cacatcagcg cggtattcgt attctctttg atgtcgtgat gaaccacacc agctatgcca
    16081 cgctggcgga tatgcaggag tatcagtttg gcgcgttata tctttctggt gacgaagtga
    16141 aaaaaacgct gggtgaacgc tggagcgact ggaaacctgc cgccgggcaa acctggcata
    16201 gctttaacga ttacattaat ttcagcgaca aaacaggctg ggataaatgg tggggaaaaa
    16261 actggatcag aacggatatc ggcgattacg acaatcctgg attcgacgat ctcactatgt
    16321 cgctggcctt tttgccggat atcaaaaccg aatcaactac cgcttctggt ctgccggtgt
    16381 tctataaaaa caaaacggat actcacgcta aagtcatcga aggctttacg cctcgcgatt
    16441 acttaaccca ctggttaagt cagtgggtcc gcgactatgg gattgatggt tttcgggtcg
    16501 ataccgccaa acatgttgag ttgcccgcct ggcagcaact gaaaaccgaa gccagctccg
    16561 cgcttcgcga atggaaaaaa actaaccccg acaaagcatt agatgacaaa cctttctgga
    16621 tgaccggtga agcctggggc cacggcgtga tgcaaagtga ctactatcgc cacggcttcg
    16681 atgcgatgat caatttcgat tatcaggagc aggcggcgaa agcagtcgat tgtctggcgc
    16741 agatggatac gacctggcag caaatggcgg agaaattgca gggtttcaac gtgttgagct
    16801 acctctcgtc gcatgatacc cgtctgttcc gtgaaggggg cgacaaagca gcagagttat
    16861 tactattagc gccaggcgcg gtacaaatct tttatggcga tgaatcctcg cgtccgttcg
    16921 gtcctacagg ttctgatccg ctgcaaggta cacgttcgga tatgaactgg caggatgtta
    16981 gcggtaaatc tgccgccaac gtcgcgcact ggcagaaaat cagccagttc cgcgcccgcc
    17041 atcccgcaat tggcgcgggc aaacaaacga cactttcgct gaagcagggc tacggctttg
    17101 ttcgtgagca tggcgacgat aaagtgctgg tcatctgggc tgggcaacag tgacttttcc
    17161 ggcttcccgt tcgtcagtac ctcgggaagc cgccaaccag gataaaatgt cagccctaat
    17221 cagcgttgcg ggataaagca ccgctcactc ttcaacagac cgatttgcac cccagcaaat
    17281 gtagcgttat tgttaccttc cttgctacag agttcgacag atatcccgct atgacattct
    17341 ccctttttgg tgacaaattt acccgccact ccggcattac gctgctgatg gaagatctga
    17401 acgacggttt acgcacgcct ggcgcgatta tgctcggcgg cggtaatccg gcgcagatcc
    17461 cggaaatgca ggactacttc cagacgctac tgaccgacat gctggaaagt ggcaaagcga
    17521 ctgatgcact gtgtaactac gacggtccac aggggaaaac ggagctactc acactgcttg
    17581 ccggaatgct gcgcgagaag ttgggttggg atatcgaacc acagaatatt gcactaacaa
    17641 acggcagcca gagcgcgttt ttctacttat ttaacctgtt tgccggacgc cgtgccgatg
    17701 gtcgggtcaa aaaagtgctg ttcccgctgg caccggaata cattggctac gctgacgccg
    17761 gactggaaga agatctgttt gtctctgcgc gtccgaatat tgaactgctg ccggaaggcc
    17821 agtttaaata ccacgtcgat tttgagcatc tgcatattgg cgaagaaacc gggatgattt
    17881 gcgtctcccg gccgacgaat ccaacaggca atgtgattac tgatgacgcg ctggcgaatc
    17941 aacacggcat tccgctggtg attgataacg cttatggcgt cccgttcccg ggtatcatct
    18001 tcagtgaagg gcgcccgcta tggaatccga atatcgtact gtgcatgagt ctttccaagc
    18061 tggggctgcc tggctcccgc tgcggcatta tcatcgccaa tgaaaaaatc atcactgcca
    18121 tcaccaatat gaacggcatt atcagcctgg cacctggcgg tattggtccg gcgatgatgt
    18181 gtgaaatgat taagcgtaac gatctgctgc gcctgtctga aacagtcatc aaaccgtttt
    18241 actaccagcg tgttcaggaa actatcgcca tcattcgccg ctatttaccg gaaaatcgct
    18301 gcctgattca taaaccggaa ggagccattt tcctctggct atggtttaag gatttgccca
    18361 ttacgaccga gcagctctat cagcgcctga aagcacgcgg cgtgctgatg gtgccggggc
    18421 acaacttctt cccgggactg gataaaccgt ggccgcatac gcatcaatgt atgcgcatga
    18481 actacgtacc agagccggag aaaattgagg cgggggtgaa gattctggcg gaagagatag
    18541 aaagagcctg ggctgaaagt cactaaaaat tgcctgatgc gctacgctta tcaggcctac
    18601 atgatttctg caatatattg aatttggaag aatttgtagg ccggataagg cgtttacgcc
    18661 gcatctggca taaacaaagc gcactattcc ggcgttgaga aacgccggaa aacgtttcaa
    18721 acaggctgct gctgtagccg atgccgctgc actttctcga catccatgca ctgcaacgcc
    18781 tttgtcgggc aggcttcaac acaggccggc cccgtctccc gatgccagca cagatcgcac
    18841 ttaatcgccc tcgccttttt gcgcgaagaa acgacctcca tcgcaccaaa cgggcaagcc
    18901 agcatacagc ttttacagcc aatgcaacgt gtttgttcaa cgaaaatatg cccatgttcg
    18961 cggcttatcg cgtcaacagg gcagacattc gcgcacggtg catcttcaca ctgatgacag
    19021 gctactggcg tggtccagct gtggtcttta atgacacgaa tacgggaaat aaactcgtct
    19081 ggtgacaacg cagcgcaatc ctgattctcc tgatgcgaca ctgcgcaagc cacttcacag
    19141 gtacggcaac cgatacattt cgtcgcatcc gcaataataa accggttcat gatcattcct
    19201 ttgcactaaa acagcagagt gccagagagc gcgtccctac tgctttgatc caacgaagga
    19261 aatgttaaag ttttggcact tgtccaaagc gttatgtgat cgcgcccagg tcatccctga
    19321 cagtaaatcc cagttcatta gaaatagcct gcgcggtttc acgcagtggt ttcaggagat
    19381 ttttctctcc cacctgtttc agacgtgatg tcgaaagcga aatcgacacg gcgtacggca
    19441 cccgcccatg aatatcaaac accggaacag caatacagga gacgccgagt tcgttttctt
    19501 ctctgtccat cgccgctccg ctttcacgaa tgtgcgccag ttcgtcgaac atcgcgggca
    19561 gctcggtaat ggtattgcgg gttaacggct ggatctcatg ctgatggttt tcccagtatg
    19621 acttcacgta gtccgggtga ccaaacgcca tgtagatctt gcccattgcg gaacagtaga
    19681 gcggcatatg ctggccaata taggcacggg ttcgcagcat cccggttgtg ggttccagct
    19741 tataaatcaa aatagcgtga tcgtcttcgc ggctggagaa gttaatggtt tcaccagtgg
    19801 cgatgttcag tgcctcaaga tgcggagcgg cgatatgaat gatattcagc gaagacagcg
    19861 ccttctgccc gacggcaata aatttggtag tcaggcggta actccctgcg gcgggcgcgg
    19921 tggtcacata gccgcaggac tgtaatccct gcaataagcg atggacggta ctcttattta
    19981 aaccagccag ctccgaaaga tgcgccaacg gacaaccgtt tggatagttg ctcaaaatct
    20041 caatcagcat caacccgcga aacagactct ggcttccggc tggacgctct ttttcctgcg
    20101 ccatctcgtt ctcttttttt cccatcactt ctttccccat tttgtcgcgt cctgatggta
    20161 gcgcaaagtg tgccgtagtt cacgatctcg acagataatt tataaccact tgatttttat
    20221 gtcttttgaa attcatcaat cagattgctt tgttaaaaag tgatcgatat atttgaaatc
    20281 tagttccgca tattgaaatt ttaagccaaa aaagcgatca aaaaaacaag gaagcctagg
    20341 atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat ttcacgcggc
    20401 gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac cgaatccggc
    20461 gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga aaacggcgat
    20521 atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga acagtgggat
    20581 gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc cattgaactg
    20641 gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg gatgcgcggc
    20701 ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg gaccaactcc
    20761 atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa cccgctgatc
    20821 gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgttctc ttacggcatg
    20881 ttagaagtta accgcctggc aggccgtcag ctcccggtcg atggtggctt tgatgatgag
    20941 ggcaatttga ccaaagaacc tggcgttatc gagaagaatc gccgcatttt gccgatgggc
    21001 tactggaaag gttctggcat gtcgattgtg ctggatatga tcgctactct cctttccgac
    21061 ggcgcatcgg ttgccgaagt cacccaggac aacagcgacg aatacggcat ttcacaaatt
    21121 tttattgcca ttgaagtgga caagcttatc gacggtccca cccgcgatgc caagctgcaa
    21181 cgtatcatgg attacgttac taccgctgaa cgcgctgacg aaaaccaggc catccgctta
    21241 cccggccacg aatttactac cctgctggcc gaaaaccgcc gtaacggcat cactgttgat
    21301 gacagcgtgt gggccaaaat ccaggcgtta tgaggagata agtcatgatt tttggacata
    21361 tcgcgcagcc caatccgtgc cgtttgcccg ccgccattga aaaggcgctc gattttctgc
    21421 gcgctaccaa cttcaacgtc ctggagccgg gcgttgtcga aatcgacggc aagaatattt
    21481 atgcgcaaa
//
LOCUS       DAJTRB010000070        21276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTRB010000070 DAJTRB010000000
VERSION     DAJTRB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.070"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..447)
                     /locus_tag="OO330_002789"
     CDS             complement(<1..447)
                     /locus_tag="OO330_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5883593.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO330_002790"
     CDS             complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO330_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5883594.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO330_002791"
     CDS             complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO330_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5883595.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO330_002792"
     CDS             complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO330_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5883596.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3663..5213
                     /gene="yoaE"
                     /locus_tag="OO330_002793"
     CDS             3663..5213
                     /gene="yoaE"
                     /locus_tag="OO330_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5883597.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5217..6815)
                     /locus_tag="OO330_002794"
     CDS             complement(5217..6815)
                     /locus_tag="OO330_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5883598.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO330_002795"
     CDS             complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO330_002795"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5883599.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8494..9072)
                     /locus_tag="OO330_002796"
     CDS             complement(8494..9072)
                     /locus_tag="OO330_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5883600.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO330_002797"
     CDS             complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO330_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5883601.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10511..10690
                     /locus_tag="OO330_002798"
     CDS             10511..10690
                     /locus_tag="OO330_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5883602.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10810..11169)
                     /locus_tag="OO330_002799"
     CDS             complement(10810..11169)
                     /locus_tag="OO330_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5883603.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11531..11875)
                     /locus_tag="OO330_002800"
     CDS             complement(11531..11875)
                     /locus_tag="OO330_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5883604.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12007..13917
                     /locus_tag="OO330_002801"
     CDS             12007..13917
                     /locus_tag="OO330_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5883605.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13975..14670
                     /gene="tsaB"
                     /locus_tag="OO330_002802"
     CDS             13975..14670
                     /gene="tsaB"
                     /locus_tag="OO330_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5883606.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14710..15291
                     /gene="yeaY"
                     /locus_tag="OO330_002803"
     CDS             14710..15291
                     /gene="yeaY"
                     /locus_tag="OO330_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5883607.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15496..17181
                     /gene="fadD"
                     /locus_tag="OO330_002804"
     CDS             15496..17181
                     /gene="fadD"
                     /locus_tag="OO330_002804"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5883608.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPELVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17263..18378
                     /gene="rnd"
                     /locus_tag="OO330_002805"
     CDS             17263..18378
                     /gene="rnd"
                     /locus_tag="OO330_002805"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5883609.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO330_002806"
     CDS             complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="OO330_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5883610.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO330_002807"
                     /pseudo
     CDS             complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="OO330_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20412..20978)
                     /locus_tag="OO330_002808"
     CDS             complement(20412..20978)
                     /locus_tag="OO330_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5883611.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21190..>21276
                     /locus_tag="OO330_002809"
     CDS             21190..>21276
                     /locus_tag="OO330_002809"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5883612.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5270 a         5273 c         5461 g         5272 t
ORIGIN      
        1 tacgagaagc ttataaattt tttccagatc gtcgatattt cgaacgcgga ttaacagccg
       61 acgctgttct aattgcatca ccaacacgcc atcttccgac aagttcatcg ctttgattcg
      121 gctatattca atccagacat tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg
      181 aatgcggatc cagaatatat aaaaacccat cagagccagt gcacttaata accatgttgt
      241 tattagcgcg ccatgattcg tgacgttgtt atagataaga atgacaatca atccgacgaa
      301 gataacgcta tcgatgcgac cacgccggag caaaggaatt gccagcaggg tggggccgtt
      361 acggcggggc atgatgaact gatcgtagat cgcgaaggcc aggagtgcgg cgatgaaaag
      421 aatcagtacc aggtccgtga ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg
      481 ccccggtagt gtacaacagt cttacagtcc cagcaggccg caagcgtaac cagcgatacc
      541 gatgacgaag aagccaacga tgatccacag cgggttaact tttttgcgca gcagccacat
      601 acaagcaaat gtcagcagca gtggtacaag gcctggcatt aactggtcaa gaatagtttg
      661 cacggtggtg acacgggtct gtccatcctg cccggtgatg gtcgaaacca ccagcgggat
      721 gttgacatgt gtccacttgt taaccaatgc ccccatgaca aacaggccga ggatagacgc
      781 cccttccgtc agtttttgca ggaagccacc acccatatct ttaacgatat cgataccttt
      841 ggagtaacca tacgctacgc cgtagtaacg ggttgccaga cgcactaggt taaacaggat
      901 gaagaacagc agcggaccta acaggctgcc gctcatcgcg ataccggcac ccagtgctgc
      961 aaataccgga cgtacggttc cccagaagat cgggtcgcct acaccagcca gcggccccat
     1021 caaaccgact ttgataccgt tgatagcacc gtcatcgatc tctgcgccat tagcacgctg
     1081 ttcttccagc gccagggtta cgccgagaat cggtgcagcc acgaacggct gggtgttaaa
     1141 gaactccagg tgacggcgaa tagcttgttt acgagcttcg ttgttctcag ggtagaggcg
     1201 acgaattgcc ggtaccatag agaagcagaa acccagtgcc tgcatacgtt cgaagttcca
     1261 tgaaccctgg aagaggttag aacgcaggaa tacgccacga atatcacttt gagtgagttt
     1321 tttctcggtg gtagtttgag ttgtatcaac catttcgctc acctgttagt ccagttcgtt
     1381 atcgagatcg ttgttaccag ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg
     1441 gctaagttgg atgtagagca ctgccataac agtaccaatc acacccagag caaccaggtt
     1501 aaagttggtg aatgctgcgg ttacgaagcc gaggtagaag aacggcatca ggtagccagc
     1561 acgcatcatg ttgataacca tcgcataacc aaccaccacg atcatgcccc cggcgatatt
     1621 cagaccattg gtcaccactt ccggaatcgc attcagcatg ttctgtactt cgctggtacc
     1681 aacagacagc gcaacgatga cggccggaat agccacacgc attgcttgca ggaacagaga
     1741 agaaacgtgg atccaggaaa tcgctgtcag gttgccgtta tcagcagcct tatccgcagc
     1801 gtgctggaaa gcaacggtaa tagtacgaac gatgatggtc agtacctggc ccgcagcggc
     1861 cagagggatt gccagtgcga tacctgcacc aatgctctga tgacctgcga taaccagaat
     1921 ggtagaaatg atagaagcca gagcggcgtc aggcgcaact gcagcaccga tgttcatcca
     1981 gcccagcgcg atcatttcca gcgtaccacc gataataata ccggttttca tatccccaag
     2041 aacgataccc accagggtac acgcgattag cggacggtga aactgaaatt catcgaggat
     2101 tgatcccata cctgcgatac aggctacgat aaataccagc acaatttgaa gagtggtaat
     2161 ctccattgta cttctcctgt ttacgtaaga cttaagtgtg aaaactgaat gataatcaac
     2221 acaatacgtt acttatcgat tttgctgatc agatccatca ttttcagttt cggatcggtg
     2281 gaaaccttac ggacttccag ctcaataccg cgtgcattca gttttttgaa cgcctcgata
     2341 tctttttcat caaccgaaac cgcgttattc acctgggttt taccctgacg gaatgccata
     2401 ccaccgacgt taacagaggt gattttcacg ccgccttcaa cgagacgctc tacatctgtt
     2461 gggttggtaa ataacagcat tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga
     2521 atcattttgg caacatcaac tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc
     2581 agtgtcttac gaacggtatc cgcagccact tcatcactaa caacaataat acgggagaca
     2641 ttggtttctt tggtccagcg ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacgc
     2701 gcaaggccaa taaccatgta gtcgtttggc cccattggtt ttgccggagt tggagccgct
     2761 tttggtgctg cggcagcggg tgctggcgcg gctttttcaa ccggtttggc tttcagtgct
     2821 ttcacgcctt cacggcctgt ttctactgcc agcgcaacca gttcatcaaa gctcgggtca
     2881 tcatcacggg ccattaacgt ttccacgagc attggaatgt taacgcctgc aatgacttca
     2941 taatgctctt tgtcgacgac aatgcggctg gcagcattga acgggctgcc tccccatgta
     3001 tcaacgagaa acagcacgcc tttagtggtg tcgagttttg ccaactgagc gttgtatttt
     3061 tcaatcagcg tttcggcatt ttcacctgga acgaaatcga tccagccgac gttttcctgc
     3121 tcgcctaaca gcatttctgc cgttttaagc aactgctctg cagcccaacc atgtgtgcct
     3181 ataacaatag caatggtcac ttgctacctc ctttattatc gttaacacct caacgtgcca
     3241 gatgtatttt tgaatcgtcg tccacaatcg aatcgattca gataagggct acaaccaaaa
     3301 acccctgtgt ttcgcgattt attttagata tcgaaaaaat tattttatgt gatgaagatc
     3361 cgtaatttaa ctttcgatta tcagaaattt cgaaaggtaa aatatccttg tcacattcgt
     3421 ttgcaaagga aggtaaatct ttgccaaatc agaggcgtct ctgatatgtt taactcccgt
     3481 ttaacaacca tggagtatag ggcagtagcc cgcagtatgg atcgtcaccg acgtcatttc
     3541 agcatcaggc cttttaacgc ctgcctttct ggcactctat gccgcacctt tcgtttgcat
     3601 tttgtcgtta cgcctgcatt atttctggcg tcgaatagct attccttaag caggagcttg
     3661 tcatggaatt cttaatggac ccctcaattt gggcggggct actcacgctt gttgttctcg
     3721 aaattgtgct gggtatcgat aacctggtct tcatcgccat tcttgctgac aaactgccgc
     3781 caaaacaacg cgataaagcg cgtttgctgg ggttatcact ggcgctgatt atgcgtctgg
     3841 ggctgctgtc gctgatttca tggatggtca cgctgaccaa accgctattt accgtcatgg
     3901 atttctcctt ctccggacgc gatctgatta tgttgttcgg ggggatattc ttgctgttca
     3961 aagcaacaac cgaactgcat gaacggctgg aaaaccgcga tcatgattcc ggccacggta
     4021 aaggctacgc cagtttctgg gtggtcgtca cacagattgt catccttgac gccgtcttct
     4081 cgttggatgc ggtaattact gcagtaggga tggttaacca tctgccggtg atgatggcgg
     4141 cggtagtgat tgcgatggcg gttatgttgc tggcatcgaa accgctgacg cgattcgtta
     4201 accagcaccc cacggtggtg gtgctctgtc tgagcttcct gttaatgatt ggtctgagtc
     4261 tggtggcaga aggtttcggt ttccacattc cgaaaggtta cctgtatgcc gcgattggct
     4321 tctcgatcat tatcgaagtg tttaaccaga ttgcgcgtcg caactttatt cgtcaccagt
     4381 cgactttgcc gctgcgagcg cgtactgccg atgccatcct gcgtttgatg ggcgggaaac
     4441 gtcaggccaa tgttcagcac gatgccgata acccgatgcc gatcccggaa ggtgcatttg
     4501 ccgaagaaga acggtacatg attaacggcg tactgacgct ggcgtcgcgt tctctgcgcg
     4561 ggatcatgac gccgcgcggt gaaataagct gggttgacgc taatctcggg gtcgatgaaa
     4621 tccgcgagca actgctctct tcaccgcaca gtctgttccc ggtatgtcgc ggtgaactgg
     4681 atgaaatcat cggtatcgta cgtgctaaag aactgctggt ggcgctggaa gagggcgttg
     4741 atgtggcggc gattgcttcg gcgtctccgg cgattatcgt cccggaaacc ctcgatccga
     4801 tcaacctgct gggcgtgctg cgtcgcgctc gcgggagctt tgttatcgtg accaacgagt
     4861 ttggtgtggt acaaggcttg gtcacgccgc tggatgtgct ggaagccatt gcgggtgaat
     4921 tcccggacgc tgacgaaacg ccggaaatca ttaccgatgg taacggctgg ctggtaaaag
     4981 gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt gccgatgacg
     5041 atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt ccccgtgtgg
     5101 gcgatgtgat tgatgtaggg ccactgcata tcaccatcct tgaagccaat gattatcgtg
     5161 ttgatctggt tcgcattgtt aaagagcaac cggcgcacga tgaagatgag taagcgttaa
     5221 cgtaacgaca taatgggcgt gatatgtcca ttatgccggg cgggcggcgg ttggctgccc
     5281 gccagccatt tgggaaaatc acgtagcggc atcggctgtg cgtacaaata cccttgcaaa
     5341 atatgcacgc catgacggcg caaatacttc gcctgttctt gcgtttccac gccctccgcc
     5401 accagttcaa tattcagtct ttgccccagc gcgatgatga tatcggtcac cgtcgagtta
     5461 accgcgtcag aacctatagc tgcggtaaat gacttatcaa ttttcagcac gtcaggacgt
     5521 aatgtttcaa gccaggaaaa cgagctgttg ccggtgccga agtcatcaat cgccagtttg
     5581 acgtttttac gatgcagctc gcgggcaatc cgataatcaa catccagtaa ggcatcgcgt
     5641 tcggtgattt cgaggatcag ttgctgaatc gggtgagcgc taaaccagta ctgattgaga
     5701 tcttttatca gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc aacatgaaac
     5761 tgactactca tcgggaaaac atggtgctga cgaatggttt ctgccatcac atagtgggtc
     5821 agtggcacaa ttaaatgatg ttcttccgcg ataggaataa acacatccgg tgaaatccag
     5881 ccctgacgcg gattattcca gcgcagcaaa atctctacac caatacattg ctggctgcgc
     5941 gcattaagca aaggctgaca gaacaattcg aactcatgtt gcgccaggcc cagattaatt
     6001 tcgcgggaaa agctcatccg gtaagcggtc gccagccagg cgatatagcc caccagtaaa
     6061 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
     6121 cccggaccat ttacgttaat ggtaaaagga aagctttgcg aagaaacctg atagttttca
     6181 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
     6241 tccaccgtca ggctggcgct actgatttgc ggcagttgtg gctcaaggag cattgccgtc
     6301 agtaagtcga tgttaatcat ctctatgacc ccagcattgc tactgcccgc tgcaggcgtc
     6361 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
     6421 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
     6481 atgctggagc aataaagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
     6541 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
     6601 agcaagactt tgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
     6661 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
     6721 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgg
     6781 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
     6841 gacaaacgcc tgacgcaaca gtggaagtgt agtggggaat attacagcag acgagaaagc
     6901 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
     6961 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
     7021 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     7081 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     7141 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     7201 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     7261 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     7321 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     7381 gtctgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     7441 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     7501 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     7561 gctggaaact aacatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     7621 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     7681 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     7741 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     7801 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     7861 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     7921 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     7981 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttt
     8041 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     8101 aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     8161 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     8221 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     8281 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     8341 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     8401 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     8461 agtagtgttt tgtagatgta aagataagta tagtcagggg ttcacaccaa tttgcagcgc
     8521 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     8581 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     8641 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     8701 cggcagatcg ggcgggataa tgccgaccac tggggttact tgatagccag ttacgctatc
     8761 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     8821 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     8881 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     8941 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttaggggttc
     9001 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     9061 acggtattcc acgtcttact tctccagttg gcgtaatatc ttattaactt tatcaaaagt
     9121 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     9181 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     9241 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     9301 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     9361 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     9421 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     9481 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     9541 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     9601 gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa
     9661 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     9721 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     9781 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     9841 ctggcgaaat ttttcgccgt actgctcgtg ggtcatattg gattgccagt cggaagtgag
     9901 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     9961 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
    10021 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
    10081 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
    10141 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
    10201 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttacctaa
    10261 agtggttaaa gtgcaaatcg gatcggcgac cacaatatca aagcggctat acggatgatc
    10321 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
    10381 ttcagcggcg tcctgacgcc agggtaaagt aatcacagtg ggagataacg tcttcatcag
    10441 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
    10501 ggagttaaca atgtttgcag gtttaccttc actcatccat gaacagcagc aaaaagctgt
    10561 cgagcggatc caggaactga tggcacaagg gatgagtagc ggccaggcaa tcgcgctggt
    10621 ggcagaagag ctgcgtgcca accactccgg cgagctaatc gtggcgcgtt ttgaggatga
    10681 agacgagtaa cacactaatc cgtatcgatt tcgttataga agccgatgat catttgattg
    10741 tgagaaaaag gacgcttttt atatgtgatt aaaagcgtcc tgacccattt atgctgatat
    10801 aaatttaact cagaccagtg cttttagttc ttcctgcata tatgttgaaa agtattcagg
    10861 atttttaatc acagagcgtt cacctgtctc catttttttt gcccatccaa ccattttttc
    10921 cgtgaaccca gggttccagc cttcctggtc agctcgcgcc gtaatatcag cggcactcgc
    10981 gggtactccc agttctttta aatatttaaa gattcccttc gccgtgcttt cattcatcga
    11041 acttggtgtt ggcgctgata catccatggc accaatgaaa tccagggctt tatctattac
    11101 agcaggcata ttcttatcct taaaattaac catgttatcg ataaggctat atatgacctt
    11161 aatttgcatc taatatcaaa gggattataa cgatatggca cgagttaacc tgttgacatt
    11221 caggtgtttt aaaggtagca gctcatggtg tgttatccag taagatggag aaaattaacg
    11281 gtcagcccgc acgctataac cagattgttc agctaagaaa gaaaaccaga gttgctaatc
    11341 aaagggcgaa cacaacggca tggttatgtt tatttatata gttagtttta tagataaaat
    11401 tgaaattata aatataaata atgaattagc agaagcatca acagtaatat cttattctct
    11461 ggtcgattta tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct
    11521 tcgataaagc ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca
    11581 aacccgcttg taccgtgcag cgcaccggcg catgacccgc gacaacccag gcatcccacg
    11641 ctttattcat cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc
    11701 tcgatttatt gctgccctgt ttttccagca cggcgtcaat ctgtgccagc gtgttggcgg
    11761 tttgctcaaa ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt
    11821 tgtggattac tacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt
    11881 tgtcctcatt atacttccat tttcgtgctg caaagactgc cacaatgtga ggttgccgtc
    11941 accacttcaa ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg
    12001 ggtagtgtga cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcttt
    12061 aagccgcgag aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc
    12121 cagccgctgg tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct
    12181 gcgctgcggg cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag
    12241 ctctacagcc gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg
    12301 ctgctgaaag ggcgctcaaa ctacctctgc ctcgaacgtc tcgaacagca ggcgctggcg
    12361 gggggcgatc tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat
    12421 caaacagtcg atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg
    12481 ccgctggtca ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat
    12541 tgctttgtgg tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat
    12601 catctctttc tggcggatat ggtggttaaa gagagtggat ttggcgagtt gatccctgaa
    12661 gcggacgtca tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt
    12721 ggtcagtcac tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcctac
    12781 cgcaccgaat taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt
    12841 gcgcaggatt ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg
    12901 ttagctaatc cgcaaattca gcgggcattt ttactgctcg atgacacctt ggaactttgt
    12961 tatgatgtgg cgaaactgtc actggggcgt tccgccttgc tggatgcggc atttgagcgc
    13021 gccacgttgt atcgcacacg gctgaagcgg ctaaaagaga tcaatcagcc gggctacagc
    13081 tactggtacg aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg
    13141 gataaattca aagagttaat ggcgcaaaag cccggtagct ggatcttcac ctcagcaacg
    13201 ctgtcggtga acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag
    13261 tcgttgctat tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc
    13321 aatctgccgc aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg
    13381 atcatcgaag ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgc
    13441 gatctggccg agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc
    13501 agtaaaggcc aactgttgca gcagtttgtc agtgccggta atgcgcttct tgtggcaacc
    13561 agtagtttct gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt aattatcgac
    13621 aaattgccgt ttacctcgcc ggatgatcca ctgttaaaag cgcgcatgga agattgtcgt
    13681 ttgcgcggtg gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa
    13741 cagggggtag gtcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac
    13801 tatcggctgg tgatgcgtcc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca
    13861 cgcacccgtg acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt
    13921 gttacggagt atggtatgat gcgcgcattt ttattgatct aagcacgaag acccatgcga
    13981 attctggcta tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact
    14041 gtcaacgctc attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg
    14101 caggatatcc tgaccaccag cggaacttcc ctgactgata ttaacgctct ggcttacggg
    14161 cgcggccccg gtagctttac cggcgtgcgc attggtattg gcatcgcgca agggctggcg
    14221 cttggcgcgg aattaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg
    14281 tggcgcaaaa acggcgcaac acgcgtgctg tcagccattg acgcgcgaat gggcgaagtt
    14341 tactgggccg aatatcagcg tgatgaaaac ggtatctggc acggtgaaga aaccgaagcc
    14401 gtactcaaac ccgaactcgt tcatgaacga atgcaacagc tttccggcga atgggtgacg
    14461 gtagggacgg gctggcaagc ctggccggat ctcggtaaag agagcgggct ggttttgcgc
    14521 gatggcgaag tgttactccc cgctgcagaa gatatgctgc cgattgcttg tcagatgttt
    14581 gccgagggta aaacggtggc ggtggaacat gccgagccgg tttatttgcg taacaacgtc
    14641 gcatggaaga aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg
    14701 agtcgcatca tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg
    14761 ctaatgctga gcggttgtgt cactgtgccg gacgccatta aaggcagcag tcccacgccg
    14821 caacaagatt tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc
    14881 tttggtggca aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct
    14941 accgtaccgc tggacagcgg agctaggccg acgctgggag aaccttctcg cggtcgcatt
    15001 tatgccgatg tgaacggttt tctggacccg gtggatttcc gtggacaact ggttacggta
    15061 gtcgggccaa tcaccggtgc ggttgacggc aaaatcggca atacgcccta taaatttatg
    15121 gtgatgcaag taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct
    15181 cagccgattg atccatggtt ttatggcggt cgtggctggc cctatggcta cggcggatgg
    15241 ggctggtata atcccggccc cgcgagagta caaacagttg taaccgaata attgcttgtt
    15301 tttaaagaaa aagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
    15361 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
    15421 gttaatatta tgttaacagc gtgtatatca tttggggttg cgatgacgac gaacacgcat
    15481 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
    15541 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
    15601 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
    15661 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
    15721 cgcgttgcgt tgatgatgcc taacttattg caatatccgg tggcgctatt tggcattttg
    15781 cgtgccggga taatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
    15841 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc ccacactctg
    15901 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
    15961 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgcttggtg
    16021 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
    16081 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
    16141 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
    16201 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
    16261 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
    16321 ggtgggcaga acctgcttat cactaacccg cgtgatattc cagagttggt aaaagagtta
    16381 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
    16441 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
    16501 cctgttcagc aagtggtggc agaacgctgg gtgaaactga ccggacagta tctgctggaa
    16561 ggttatggcc tgaccgagtg cgcgccgctg gtgagcgtta acccgtatga tattgattat
    16621 catagcggta gcatcggttt gccggtgcca tcgacggaag ccaaactggt ggatgacgac
    16681 gacaacgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
    16741 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
    16801 accggcgaca tcgcggtgat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
    16861 gacatgattt tggtttccgg ttttaacgtc tatcccaacg agattgaaga tgtcgtcatg
    16921 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
    16981 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
    17041 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
    17101 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
    17161 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
    17221 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
    17281 gcgctggctt ctttgtgtga agccgtccgt gtctttccgg cgatagccct ggatactgaa
    17341 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
    17401 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
    17461 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
    17521 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
    17581 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
    17641 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
    17701 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
    17761 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
    17821 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
    17881 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
    17941 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
    18001 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
    18061 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agatgcctta
    18121 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt taaagcgatt
    18181 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
    18241 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
    18301 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
    18361 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
    18421 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
    18481 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
    18541 tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
    18601 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
    18661 aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt gtcttcgata
    18721 gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
    18781 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
    18841 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa atggactttc
    18901 tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg atttcgcgag
    18961 cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg cgacaggaaa
    19021 ggggtgatac caataccgcc cgcgataaac agatgctttc tggctgaggg aattagcgca
    19081 aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg ctgatgcaaa
    19141 aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata actagaggtg
    19201 ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
    19261 tgcactagga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
    19321 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
    19381 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
    19441 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
    19501 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
    19561 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gatggaacac
    19621 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
    19681 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
    19741 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
    19801 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
    19861 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
    19921 gaaacctaga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
    19981 ccagttggca ggcgtgcggg tggtaaagcg ggccgctagt ttcagattgt ggacttccgg
    20041 gcaggcttcc agcactttcg cgcccagacc ggtgtattga ttcacttgaa gtacgaaaaa
    20101 aaaccgggag gacattggat tattcgggtt ctgatgggat tagatttggt ggggcttgca
    20161 agcctgtagc gcaaatttta gtcgttaatc aatgaaacgc gaaagatagt aaaaaattgc
    20221 ttttgtttca ttgaaaatac gaaaaacaaa aacactgcaa atcatttcaa taacagcttc
    20281 aaaaaacgtt caaaaccgat aacaactagg ctgtaaccaa atgactcata tcacaaatca
    20341 gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg
    20401 gttcatggtt gttatacggc tttttttaca tcttgtggtt cttgtgaagg agctacaaga
    20461 ccttcctgtt cataaatacc atactttgac aaaatctttt tacgagtatc tgttggtaat
    20521 gattcaagca ttttggctgt ttgtaaggtt tgctgcgaaa taccttgcaa atcagcctgg
    20581 atggaatcat cggaagaaaa aatctcaggc ataagagctt caaatccttt ctttctgaac
    20641 aactcaatcg cagaatgcag ttccttgtcc tctaacaatt cgacaattgc gatcagttta
    20701 gttttcaatg tgctgcggtc aggttgagaa tttttattct cttgattatt tttatgctca
    20761 accattggca tgctctgact ttggataaac cactctattg gacgccctgt tgcgtaggct
    20821 atttgtgcta atcgatctaa agacgggtat gtcttgccag acaggtagtt tctgataacc
    20881 ccatcagaaa gtccgcactt agctgcaaac gcccggcttg attctccatt cattgcctct
    20941 tcaaggcgtt ccttgaaacg cattatttcc gggggcataa aaatgcgctc tgcatttttg
    21001 ctttcttttt cacctgtcat gcccaatctc tctgttatat aaagaatttt acagaattct
    21061 ttgatttgca ctggttaaaa taaaaatgcg catagctctt gaattgagca tttttctgcg
    21121 atatcgtttt tcccgatagc ggtagccggt atcagttacc gaaaacgtta agagagtaag
    21181 gatcgcagaa tgtcagcaaa aaaccaagac tggcacgcag agcagataaa ggctgctgtt
    21241 cgtatgaagg ggaagacact atctcaatta tccaga
//
LOCUS       DAJTRB010000071        21253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTRB010000071 DAJTRB010000000
VERSION     DAJTRB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.071"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            18..791
                     /locus_tag="OO330_002810"
                     /pseudo
     CDS             18..791
                     /locus_tag="OO330_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO330_002811"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO330_002811"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5883613.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO330_002812"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO330_002812"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5883614.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO330_002813"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO330_002813"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5883615.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OO330_002814"
     CDS             complement(4989..7223)
                     /locus_tag="OO330_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5883616.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO330_002815"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO330_002815"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5883617.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OO330_002816"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OO330_002816"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5883618.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGGIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO330_002817"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO330_002817"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5883619.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OO330_002818"
     CDS             complement(12978..14318)
                     /locus_tag="OO330_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5883620.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NTGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO330_002819"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO330_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5883621.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OO330_002820"
     CDS             complement(16106..16555)
                     /locus_tag="OO330_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5883622.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO330_002821"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO330_002821"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5883623.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OO330_002822"
     CDS             complement(17355..17684)
                     /locus_tag="OO330_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5883624.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO330_002823"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO330_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5883625.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OO330_002824"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OO330_002824"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5883626.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OO330_002825"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OO330_002825"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5883627.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5225 a         5667 c         5073 g         5288 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctggtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tagttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaatg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagagctatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcggta ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggtgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acctaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaatgc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccgc tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DAJTRB010000072        20891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTRB010000072 DAJTRB010000000
VERSION     DAJTRB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.072"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..255
                     /gene="mliC"
                     /locus_tag="OO330_002826"
     CDS             <1..255
                     /gene="mliC"
                     /locus_tag="OO330_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5883628.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            314..970
                     /gene="pdxH"
                     /locus_tag="OO330_002827"
     CDS             314..970
                     /gene="pdxH"
                     /locus_tag="OO330_002827"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5883629.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1099..2373
                     /gene="tyrS"
                     /locus_tag="OO330_002828"
     CDS             1099..2373
                     /gene="tyrS"
                     /locus_tag="OO330_002828"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5883630.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRVQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2432..3295
                     /gene="pdxY"
                     /locus_tag="OO330_002829"
     CDS             2432..3295
                     /gene="pdxY"
                     /locus_tag="OO330_002829"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5883631.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3339..3944)
                     /gene="gstA"
                     /locus_tag="OO330_002830"
     CDS             complement(3339..3944)
                     /gene="gstA"
                     /locus_tag="OO330_002830"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5883632.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4050..5552)
                     /gene="dtpA"
                     /locus_tag="OO330_002831"
     CDS             complement(4050..5552)
                     /gene="dtpA"
                     /locus_tag="OO330_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5883633.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6163..6798)
                     /gene="nth"
                     /locus_tag="OO330_002832"
     CDS             complement(6163..6798)
                     /gene="nth"
                     /locus_tag="OO330_002832"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5883634.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6798..7493)
                     /gene="rsxE"
                     /locus_tag="OO330_002833"
     CDS             complement(6798..7493)
                     /gene="rsxE"
                     /locus_tag="OO330_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5883635.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7497..8117)
                     /gene="rsxG"
                     /locus_tag="OO330_002834"
     CDS             complement(7497..8117)
                     /gene="rsxG"
                     /locus_tag="OO330_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5883636.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8121..9179)
                     /gene="rsxD"
                     /locus_tag="OO330_002835"
     CDS             complement(8121..9179)
                     /gene="rsxD"
                     /locus_tag="OO330_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5883637.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9180..11402)
                     /gene="rsxC"
                     /locus_tag="OO330_002836"
     CDS             complement(9180..11402)
                     /gene="rsxC"
                     /locus_tag="OO330_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5883638.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11395..11973)
                     /gene="rsxB"
                     /locus_tag="OO330_002837"
     CDS             complement(11395..11973)
                     /gene="rsxB"
                     /locus_tag="OO330_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5883639.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11973..12554)
                     /gene="rsxA"
                     /locus_tag="OO330_002838"
     CDS             complement(11973..12554)
                     /gene="rsxA"
                     /locus_tag="OO330_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5883640.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12631..13071)
                     /locus_tag="OO330_002839"
     CDS             complement(12631..13071)
                     /locus_tag="OO330_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5883641.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13157..13372)
                     /gene="ydgT"
                     /locus_tag="OO330_002840"
     CDS             complement(13157..13372)
                     /gene="ydgT"
                     /locus_tag="OO330_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5883642.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13645..13770)
                     /gene="blr"
                     /locus_tag="OO330_002841"
     CDS             complement(13645..13770)
                     /gene="blr"
                     /locus_tag="OO330_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5883643.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14013..15053
                     /locus_tag="OO330_002842"
     CDS             14013..15053
                     /locus_tag="OO330_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5883644.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15089..16090)
                     /gene="add"
                     /locus_tag="OO330_002843"
     CDS             complement(15089..16090)
                     /gene="add"
                     /locus_tag="OO330_002843"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5883645.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16194..17366)
                     /gene="malY"
                     /locus_tag="OO330_002844"
     CDS             complement(16194..17366)
                     /gene="malY"
                     /locus_tag="OO330_002844"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5883646.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17376..18968)
                     /gene="malX"
                     /locus_tag="OO330_002845"
     CDS             complement(17376..18968)
                     /gene="malX"
                     /locus_tag="OO330_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5883647.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19143..20171
                     /gene="malI"
                     /locus_tag="OO330_002846"
     CDS             19143..20171
                     /gene="malI"
                     /locus_tag="OO330_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5883648.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20283..>20891
                     /gene="hdhA"
                     /locus_tag="OO330_002847"
     CDS             20283..>20891
                     /gene="hdhA"
                     /locus_tag="OO330_002847"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5883649.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PSDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
BASE COUNT         5321 a         5258 c         5362 g         4950 t
ORIGIN      
        1 gagcgaatgc aaaccgatac gctggaatac cagtgtgatg aaaaaccgtt gacggtcaaa
       61 ctgaataatc cgcgccagga agtcagtttt gtttacgata atcaactact gcatctcaaa
      121 cagggccttt cagcctctgg cgcgcgttac tccgacggga tctatgtttt ctggtcgaaa
      181 ggtgaggaag cgactgtcta taaacgcgac cgcatcgtct tgaataactg tcagttacaa
      241 aatccacagc gttgagattt ttccaggggc ggcgcacaat agcgtcaccc actgacaatc
      301 cgtaaagaaa accatgtctg ataacgacga attgcagcaa atcgcgcatc tgcgccgtga
      361 atacaccaaa ggcgggttac gccgccgcga tcttcccgcc gatccattaa ccctttttga
      421 acgttggctc tctcaggctt gtgaagccaa actggcggac cctaccgcga tggtggtcgc
      481 taccgtggat gaacatggtc agccttatca gcgcatcgtt ttactcaaac attacgatga
      541 aaaaggcatg gtgttttaca ccaacctcgg tagccgtaaa gcacatcaaa ttgaaaataa
      601 tccgcgcgtt agcctgctgt tcccgtggca tacccttgaa cgccaggtga tggtgatcgg
      661 taaagcagaa cgactttcga ctctcgaagt gatgaaatat tttcatagcc gcccgcgtga
      721 tagccagatt ggtgcatggg tttcgaagca gtccagtcgc atttctgccc gcggtatcct
      781 tgaaagtaaa ttcctggagc tgaagcagaa gtttcaacag ggcgaagtgc cattgccgag
      841 cttttggggc ggttttcgcg tcagccttga acagattgag ttctggcagg gcggtgagca
      901 tcgcctgcat gaccgctttt tgtaccagcg tgaaaatgat gcgtggaaga ttgatcgtct
      961 tgcaccctga aaagatgcaa aaatcttgct ttaatcgctg gtactcctga ttctggcact
     1021 ttattctatg tctctttcgc atctggcgaa aagtcgtgta ccggcaaagg tgcagtcgtt
     1081 atatacatgg agattttgat ggcaagcagt aacttgatta aacaattgca agagcggggg
     1141 ctggtagccc aggtgacgga cgaggaagcg ttagcagagc gactggcgca aggcccgatc
     1201 gcgctctatt gcggcttcga tcctaccgct gacagcttgc atttggggca tcttgttcca
     1261 ttgttatgcc tgaaacgctt ccagcaggcg ggccacaagc cggttgcgct ggtaggcggc
     1321 gcgacgggtc tgattggcga cccgagcttc aaagctgccg agcgtaagct gaacaccgaa
     1381 gaaactgttc aggagtgggt ggacaaaatc cgtaagcagg ttgccccgtt cctcgatttc
     1441 gactgtggag aaaactctgc tatcgcggcg aacaactatg actggttcgg caatatgaat
     1501 gtgctgacct tcctgcgcga tattggcaaa cacttctccg ttaaccagat gatcaacaaa
     1561 gaagcggtta agcagcgtct caaccgtgaa gatcagggga tttcgttcac tgagttttcc
     1621 tacaacctgt tgcagggtta tgacttcgcc tgtctgaaca aacagtacga tgtggtgctg
     1681 caaattggtg gttctgacca gtggggtaac atcacttctg gtatcgacct gacccgtcgt
     1741 ctgcatcaga atcaggtgtt tggcctgacc gttccgctga tcaccaaagc agacggcacc
     1801 aaatttggta aaactgaagg cggcgcagtc tggttggatc cgaagaaaac cagcccgtac
     1861 aaattctacc agttctggat caacactgcg gatgccgacg tttaccgctt cctgaagttc
     1921 ttcaccttta tgagcattga agagatcaac gccctagaag aagaagataa aaacagcggt
     1981 aaagcaccgc gcgtccagta tgtactggcg gagcaggtga ctcgtctggt tcacggtgaa
     2041 gaaggtttac aggcggcaaa acgtattacc gaatgcctgt tcagcggttc tttgagtgcg
     2101 ctgagtgaag cggacttcga acagctggcg caggacggcg taccgatggt tgagatggaa
     2161 aagggcgcag acctgatgca ggcgctggtc gattctgaac tgcaaccttc ccgtggtcag
     2221 gcgcgtaaaa ctatcgcctc caatgccatc accattaacg gtgaaaaaca gtccgatcct
     2281 gaatacttct ttaaagaaga agatcgtctg tttggtcgtt ttaccttact gcgtcgcggc
     2341 aaaaagaatt actgtctgat ttgctggaaa taatgcatta agtggaaagg gggagtgaga
     2401 aatcactccc cctggttttt atacagggaa catgatgaaa aatattctcg ctatccagtc
     2461 tcacgttgtt tatggtcatg cgggtaacag tgcggcagag tttccgatgc gccgcctggg
     2521 cgcgaacgtc tggccgctga acaccgttca attttctaat cacacccaat acggcaaatg
     2581 gactggctgc gtgatgccgc ccagccattt aaccgaaatt gtgcaaggca ttgccgccat
     2641 tgataaatta cacacctgtg atgccgtatt aagtggctat ctgggatcgg cggagcaggg
     2701 tgaacatatc ctcggtatcg tccgtcaggt gaaagcagcg aatccgcagg cgaaatattt
     2761 ttgcgatccg gtaatgggtc atccggaaaa aggctgtatc gttgcaccgg gtgtcgcaga
     2821 gtttcatgtg cggcacggtt tgcctgccag cgatatcatt gcgccgaatc tggttgagct
     2881 ggaaatactc tgtgagcatg cggtaaataa cgtcgaagaa gcggttctgg cagcgcgcga
     2941 actcattgag caagggccac aaattgtgtt ggttaaacac ctggcgcgag ctggctacag
     3001 ccgtgaccgt tttgaaatgc tgctggtcac cgccgatgaa gtctggcata tcagccgtcc
     3061 actggtggat tttggtatgc gccagccggt aggtgttggt gatgtgacga gcggtttact
     3121 gctggtgaaa ctgcttcagg gggcaacgct gcaggaggcc ctggaacatg tgaccgctgc
     3181 agtctacgaa atcatggtga ccaccaaagc aatgcaggaa tatgagctgc aagtggtggc
     3241 tgctcaggat cgtattgcca aaccagaaca ttacttcagc gcaacaaagc tctgaaatat
     3301 ctcatttcgg cccacaacga cctgtgggcc gatggctatt actttaagcc ttccgctgac
     3361 agcgcgtctt gtacttccgg acgttcagcc atacgttgca taaatgccgc aatgtgctct
     3421 aacccttcca gattcagttt caccgcgtat gcccagcgca gaaccgtaaa cagataggca
     3481 tcagcaattg taaatctttg cccgcagatc cagtgctcat ccttcagtgc ctcgttcaca
     3541 tattgcagct tcttatccag ctgcgcgcga actgtcggtt tgtactcttc cggtgtatct
     3601 gggcgaaaca gaggtgtgaa acctttatgc agctcggtgg cgatgtaatt cagccattcg
     3661 atggttttat agcgggaaat actgtttacc ggtgccagca actggcggtc ggggacgctg
     3721 tcggcaagat actgcataat cgcaacgcct tccgtcagca aagtaccatc gtccagcagc
     3781 aatgcaggca cctgcccctt agggttaacg gaaaagtaat cgtcaccgtt ttcgagacgt
     3841 tttttcatta aatccacact gacgagcgta aaatcctttc cgctctcacg cagggtgata
     3901 tgggaagcga gagagcaggc gcccggtttg tagaacaatt tcatcggtaa ctccttttgt
     3961 ctgtggttta aagtatggta gtgcgttgct gaacaaaaaa aaagccgcta acgaaaagtt
     4021 agcggcttgt aaaagagttt ccctgaattt tacgctacag tagctttcgc cgctttgtct
     4081 gcagcgtcat cctgcgtcat gcggtgcagt ttaggcgcgg tcagcagcat cagtactgca
     4141 ataacggcag tagcgacacc aatctgcaag aatacgcgac catagacttc cagtgacatc
     4201 agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc accaatcagg
     4261 tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag acgctgcgga
     4321 acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc gatgctctgc
     4381 aggccatagc ttgcgaccag ccagctgaca gacacgatac ctgcgtcaga cgcgaatttc
     4441 gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat tgcaaacttg
     4501 gttggcatcg gcagggtatc acccatcttg ttatagatag cggccagaat cggactaccg
     4561 atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc cagacccaga
     4621 atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat ctggctgtac
     4681 agcacgaaga agatgattgc ttcgagcatc aggatgaagg caacgatcat tttacgacgc
     4741 gcagcacctt tcatcgcgaa agcttcttta ccgaagataa ccacaatacc gaaggcaaca
     4801 acgcccagcg ccatacgcgc aacttcctga ttgtgcagta gccaggtggc aacagcgatc
     4861 agcgccacaa caccaataat ggtcagcaac aggttacggt agttgactgg ttcaaagtct
     4921 ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac gatagtgatc
     4981 agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc ggccagccac
     5041 ggggtggcta tcatagagaa gaaagagccg atgttgacgg acatgtagta catggtgaat
     5101 gcaccgtcca gacgtgggtc gtttttctca tagcatgtag aaagcagaga agacgggtta
     5161 gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac aataccggcg
     5221 tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc gccgagcata
     5281 attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc gaccagacca
     5341 taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga catacccagt
     5401 tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa acgttcccat
     5461 aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt caaactgacg
     5521 ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac atcccatatt
     5581 aacgggggtg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt atagtgggag
     5641 gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt gtaataatct
     5701 gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc agcgttaaat
     5761 ttttcaaaaa acgtaacggc agattttttc actgcattga gagggggatg atggcttgac
     5821 ggcgatatgt tctgctattt agcgtttttc ctagtgagat agtccagttt ctgaaaaata
     5881 gccagtgtaa tgttttgcag gtcaatattt gttgcgtgtg taaatttaaa tcattttaac
     5941 ctgattcttg caatgttttt acattgccac agcatcgtga tcttgatcac gaatgaatag
     6001 aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca aacggattac
     6061 ctgatgataa cgctaagcaa tggcatcatt gacgttaaaa agatgtttta aaagaggata
     6121 aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt caactttctc
     6181 tttgtattca caaagatctt caataataca agagccacag cggggcttgc gggcaatgca
     6241 ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact ctgccggaac
     6301 cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa attgagtacg
     6361 attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga atgcagtgtt
     6421 taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg cagcacgatc
     6481 ttccggaacc tcgccattat gttgttccag taatatacgg caggttttga tgatattttc
     6541 tgctttgctg ttataaaggc caatcgtttt gatataggtt ttcacccctt caacgcctag
     6601 ttcaagcatc gctgcaggcg tattcgccac cgggtagagt ttcgccgtcg ccttattaac
     6661 actgatatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa aaggcgaact
     6721 gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga ggatctccag
     6781 acgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc acgttcggca
     6841 gctgcttcag cacggcgctt tttcattctt tcatcaatca ggtattttcc tgccagcatc
     6901 agtcccaagc caataaatgc acctggtggc agcatcgcca gcaggaaagg ggagtcggtg
     6961 tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc tgcaccgtca
     7021 aacaacgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat ggcgcaggtt
     7081 gcgcccgtac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt tttggcggcg
     7141 aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat aaaaatccct
     7201 aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc gctgaccacc
     7261 gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca gtgacgcagc
     7321 gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc taaacccaga
     7381 gcgttagtgg cagtggatgt gaccgccaat agcggacaaa ggccgagcag ttggaccagt
     7441 gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct cacgctttat
     7501 tctccacagg caggaagttg agaaagttgt gctggtaacg tctgagcgta caatccggcg
     7561 cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa ctggtcgaaa
     7621 tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat tttttttccc
     7681 gcaaaatggg tgagccagtc agaaaggcgc aattcgattt tatcgccaag ccctggcgtt
     7741 tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccat taaaatcggc tcccaccagc
     7801 agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc ggctaccggt
     7861 ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc tggcgcagtt
     7921 accagatagc aactctgtgc gagcgcattg ttatagcgtt cggctggcag cacctgatca
     7981 aataacgtct tttgttgcag actggcctgt tcagcaattg tcgttttggt catctggttg
     8041 atggccgcag ttaaccctgt agaacccgct gcaaatagtg ccagtgtaat gccgtgtttt
     8101 cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg acgcgtgtag
     8161 taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac gccgtcagga
     8221 tagccgccga aactgcggat caaccagact aataagcccg ccagcgcgcc gaacatcagc
     8281 cgaccacgat tggtcgtaga agcagtaact gggtcagtca aaataaagaa tgcgccgagc
     8341 atagttgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg tgagaacaac
     8401 cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat atgccagcga
     8461 atcgctttct gccatagcaa ccacacgcca ccagccagcc aggcgagatt tacccattgc
     8521 caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg ttcaaccgaa
     8581 tgacctgcgc ggacagaggt tttgaatgta tccagcggtg tcgcctgact aatgccatca
     8641 atacctaagc gtagcgtatt catatcacca ccactggcgg tatgaccgct gaaaataacc
     8701 tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg taaccagctg
     8761 gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc cggattaaac
     8821 gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa caccgtaccc
     8881 agtacgacca tccaccatgg cgctaggggg ggaatactta ccgccagcaa taagcctgtc
     8941 agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag tttgagtacg
     9001 agagcttcgg ctaacagagc actgaccgac gccaacagga tctgaacgag agtaccccaa
     9061 ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac caacagcata
     9121 atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct gaataccatt
     9181 tagtcctcgt ttacaacctt ttgctgggcg gcttttttgg cctgaacgcg ggcaatagcc
     9241 gcggcaactg cggctttgcg cggatcgacc tgttcttctg gttccgcatt agcctgttgc
     9301 tgttccagct tgcgtgcttt ggcgcgggca atagcggctt cgacggcagc tttgcgcgga
     9361 tcaacctgtt cttctggctc cgcattggcc tgttgctgtt ccagcttgcg tgctttggcg
     9421 cgggcaatag ccgcttcgac ggcagctttg cgcggatcaa cctgttcttc tggctccgca
     9481 ttggcctgtt gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggcg
     9541 gctttgcgcg gatcgacctg ttcttctggt tccgcattag cctgttgctg ttccagcttg
     9601 cgcgctttgg cacgggcaat agcggcttcg acggcggctt tgcgcggatc gacctgttct
     9661 tctggttccg cattagcctg ttgctgttcc agcttgcgcg ctttggagcg ggcgatagct
     9721 gcttcaacgg cagttttacg tggttcagca acggttgctg cgtcgttagt ttgctccagt
     9781 tctgcctgtt tcgctctggc ttgcgcttta cgggcttccc gttctgcaat aattgcactg
     9841 ttatccgggc gttcgcccgc tttaatcaca ataggctgtg tagcctgggc ctgtttctct
     9901 ttcacccgcg ccagagcggc agcaatcgca tctttatctt tggctgcagg ttgaacggct
     9961 gcgctcttat gtcgttcaag gcgagtcgct ttttcgcgct ccagacgagc ctggcgcgct
    10021 tcgaaacgcg ctttggcttc tgcggcgcgc ttttcttcct gacgaatagc cgcaatttca
    10081 gctttttcct gacggaaata ttgcaccaag ggaatattgc tcgggcaaac ccacgcgcaa
    10141 gccccacatt caatgcagtc ggcaatgtta tgcgtggtag ctttatcgtg ttgctgacct
    10201 ttgctgaacc agtacaactg ttgcggcaaa agatccacag ggcaggcgtc agcacaggcg
    10261 ctacaccgga tgcagctttg ttcttcctgt ggttcgccaa gttcattggc agagggagcc
    10321 aacagacagt tggtaatctt tacgaccggg acatccagcc atggcaaggt aaagcccatt
    10381 agcgggccac ccataatcac catttgatcg gcagaggggc agaatccggc atcattcaat
    10441 aaatgacgca ctggcgtccc cagccgtgcc cagacgttgc ccgggcgagc gattgcttcg
    10501 ccagtcaggg ttacaacacg ctcggtaatc ggctcgccat caataacggc acgtttcact
    10561 gcataagcag tgccgacgtt ttgcattaat acgccgatat cggatgaacg cccgccatgt
    10621 ggaacctgct tcccggtcag aatgtaggtt aattgtttag caccgccaga aggatatttg
    10681 gttggaatca cccgcagaga aatatcgtta gagtccgcca gcaccgcgcg cagcatggaa
    10741 atcgcctgcg gtttgttatc ttcaatgccg ataagaattt cgcgtggctg cagaatatgc
    10801 gcaagaatgc gaataccctc tacgacctga gccgcgcaat cctgcatcaa acggtcatcg
    10861 gcggtaatgt acggctcgca ctcagcagcg ttgataatca acgtttcaat cttatctcca
    10921 ccaccctgca atttaacgcc tgtcgggaat ccggcaccgc ccagcccggc aacaccaaac
    10981 tgatgtatgc gctcgattaa ctcttcgcga ctgcgagagc gataatcgac ccagccgtcg
    11041 cgcgggatcc agcagtcttc accatcggca tcaataatca cgcttaattc agctaaagct
    11101 gaaggatgag ccgtagagtg gggcgcaata gccgtaacgg tacccgaggt gggcgcgtga
    11161 acaggcagca ttttgccgcg accacgggta agcggctggc cgcgcaatac tttatcgccg
    11221 acgctaacgc acaactcacc ttcagcgcca atatgctgtt tcagtggaat aacaaaatgc
    11281 tgcgccagta gtacctggcg caggggggta ccgttggtct gggttttcat ctccggtgga
    11341 tggatgccgc cgttgaaatc ccagatttta ttttttctga atgcagagaa taacttaagc
    11401 atggtgttcc acgggaatga tacgcacggg aatggtgttc agatcccatt tccaggagtc
    11461 aggtgtttct gcgaccggtt gcaacgagat gcagtgcgtc gggcacggat caacacataa
    11521 attgcagccc gtacagagat cactcattac cgtatgcatg acacgggtag cgccaacgat
    11581 ggcgtctacc ggacaagcct gaatacattt agtgcagcca atacagttat tttcatcaat
    11641 aaccgccacc atccgcgcag gcgttagctc ttgcgcttcg ccatccagcg gctgcggctc
    11701 gacattaagc aactcggcaa tttttagcat cacagcttcg ccacctgggg cgcaacggtt
    11761 gattttttca ccgttacagc tgatggcttc cgcgtagggg cgacagccgg gataaccgca
    11821 ctgaccacac tggctctgcg gtaagatttc gtcaattttc tcaacgaccg gatcgtcttc
    11881 caccgcaaaa cggcgggagg cataacccag aatggcgcca aacgccaggc ccagcaggct
    11941 cacggcggca acggcaatcc agatagcatt cattacaact tcaccaaacc actaaagccc
    12001 ataaaggcca gagacataag acctgcggta attaacgcaa tggcattacc gcgaaaaggt
    12061 gccgggacat cagccacagc aaggcgttcg cggatggcgg cgaagagcac catcaccagc
    12121 gagaaaccga cagcggcgga aaaaccgtac agcgccgact gcaagaaatt gtgcccgaga
    12181 ttgatattca gcaacgccac gccgagcact gcacagttgg tggtgataag cggcaaaaaa
    12241 atccccagca gacggtaaag caccgggctg gttttgcgca ccaccatctc ggtgaactgc
    12301 acgaccacag caatcaccag aataaatgcc agggtgcgca ggtaaatcag attaagtggg
    12361 atcaaaatcc acgtatcgat aagccaggcg caaatagacg tcagcgtcat cacaaacgtt
    12421 gttgccagcc ccatgcccat cgcggtttcc agttttttgg aaacccccat aaacggacag
    12481 agaccgagaa acttgaccag tacaaagtta ttgaccagta cagttccgac aaacagtaac
    12541 aggtagtcag tcatttttcg gcctgaaata aaaaagccgc ctattatccg ttaatccaga
    12601 gcaggcgaca acaggttaac tgtaaggtta ttacggattc acaaacgtcg cttttacccg
    12661 cgacgagcgt ttgaaatagg ggacgatcaa tgcggttgcc aacaaagtaa acagcaactg
    12721 gcgaacggct atgccaactt ccacgggtga aaaagcgaat gcttttactg ccagcaatac
    12781 ggaaataagc agccagataa tatagtgttt aggaacacaa cgccgacgtt taaagaaagc
    12841 gatggtcaac cacagtgtgt aataccacat ggcgatagcg gtaataaaag aaacgcccca
    12901 taagatttgt gtcgtcagtg cttgtccgcc gagcgtctga aatgtttgcg gagaagataa
    12961 cgcagcggta tacagcaaca acgccagcgt cgtactcaac agagcgacta aaagccaggc
    13021 caacgggcca agcaaccagc ctccaatacg ttgtggcgtt gtggtggtca taggttctcc
    13081 agaatagcga cgcaaacagg caaatctaat tttaggggcg ttattataag gcttttataa
    13141 ccgatcgcta ctttctttat tggacatagt gccagacgga cttcggtacc tggccgaggt
    13201 caaacaaacg cccgccagaa accagctctg cgcgacggtg atcggcagca cgatacatat
    13261 tgatcagttc ctgatcgtcc gtcagggtgt aattaagatg gtcgtagagt ttttccagac
    13321 tttcgagtga actgattttg cgaaatttta ataagtagtc ctgaacagtc ataaaaggtc
    13381 cataaaataa ttaataccaa aggggtaggt attattagtg atgataaaat ttcaccaaaa
    13441 gtgaacgctt gtgttattac aataataaga atgcgctgat ggcaaatatt cagggatgaa
    13501 aaactgctct gaaaagagta acgcgcttta ctatccctga caatcaccaa caacatcgaa
    13561 caagataata aattcctggt ttaatatccg acaagtgaaa acatgcaccc ggacgggcag
    13621 catgtcgctc cacaagtgca gagcttactt gtgttgtacc gaagcactct gttcaggtgg
    13681 ctgatagttg tcaatgtgac tcgccacgcc aagaagaatg actgaaacga caagaacgat
    13741 ccaacctgtt aattcaataa gacgattcat tacagcccac atctctcttg attgatccat
    13801 taacttcagg gggtaaatgt tacttagcaa taatagctcc agcaagattt ttactgaggt
    13861 ttttgcgata tttagctttt gtcgttggaa aattcgctat ttttttgact taagttaaac
    13921 aacatccctt attgctggca ggttattaaa ctgttgagcg tgggtaaagg atagtgtcaa
    13981 atagccatca tacttcaatg agaggcaatg acatgagcga caacatccgt gttgggttga
    14041 ttgggtatgg ttatgcgagc aaaaccttcc atgcgcccct gattgcgggc acgcccgggc
    14101 tggaactggc ggtaatctcc agcagtgatg aaacaaaagt aaaagccgac tggccaacgg
    14161 ttacggttgt ctctgagccg aagcatctgt ttaacgatcc caacatagac ctgattgtca
    14221 ttcctacacc caacgatacc catttcccgt tagccaaagc ggcgcttgag gcgggtaaat
    14281 atgtggtcgt tgataaaccc tttaccgtga cactgtcaca agcgcgagag ctggatgcgc
    14341 tggcaaaaag cctggggcgt gtgctgtctg tattccataa ccgtcgttgg gatagcgatt
    14401 tcttgacgct aaaaggtttg ctcgcggaag gcgtgctggg tgaagttgct tactttgagt
    14461 ctcattttga ccgcttccgt ccgcaggtgc gcgatcgttg gcgtgaacag ggcggcccag
    14521 gcagcggtat ctggtacgat ttagcaccgc atcttcttga tcaggccatt acgctatttg
    14581 gtttaccggt cagcatgacg gtagatttgg cacagttacg gcccggagcg cagtcgaccg
    14641 attatttcca cgccatcttg tcctatccac agcggcgagt cattttacac ggtaccatgc
    14701 tggcagccgc tgagtcagca cggtatatcg tgcatggatc ccgaggcagt tatgtgaaat
    14761 atggcctcga tccacaggaa gaacgtctga aaaatggcga gcgtctgccg caggaagact
    14821 ggggctacga tatgcgtgat ggcgtactta cccgcgtgga aggtgaggaa cgtgtcgaag
    14881 aaacgctgtt gacggtgcct gggaattatc cggcttacta tgcggcgatt cgtgatgcgt
    14941 taaatggcga tggtgaaaat ccggttccgg caagccaggc aatccaggta atggagttga
    15001 ttgagctggg catcgaatcc gccaaacatc gcgcgacgtt gtgccttgcg tgaaaaatgg
    15061 atggtgcaat ttgcaccatc catttttgtt acttcgcggc gactttttct cgcagtgcgc
    15121 gtttttcctc tgcgctgagg aaagccattt ccagaccatt aatctgtgcc tggcggattt
    15181 gctcacggga taacccagca gctggcgcgg caacggtata ttcgtgaatg atatccactc
    15241 cctgtacgcc gggatcgtca gtgttaatgc tggcacgaat gccatgctca aggaacgttt
    15301 tcagcggatg tgcagccagc tctgctacgg tgctggtctg aatattggag gtcagacagg
    15361 attcaatacc aatttgttgc tcggcgagaa aatccatcag cgcccgatct tcaatggctt
    15421 ttacgccatg tccaatacgc tccgcaccca gttcacgaat cgcctgccag atgctttccg
    15481 gcccggcagc ttcgcctgca tggacggtaa tatgccagcc cgcatcacgc gcgcggttga
    15541 agtgagaaag gaacagactt cccgggaatc caagttcatc accagctaaa tcaagtgcgg
    15601 taatctggtc acggtgggct aaaaaggcct ccagctcttg ctgacaggcg gcttcgccga
    15661 aggttcggct cataatgccg ataagcttcg cctgcacacc aaaggtgcgg caaccttcac
    15721 gtacgccatc gatcaccgct tcgacaacac ccgctacagg cagcttatgt gccattgcca
    15781 tgtagcctgg tgaaaaacgc agctcgacat agcgcaggcc gttacgggct gcatcttcaa
    15841 tgttttcaaa tgccacgcgg cggcaggcat caagagaggc gagaacttta acgccccagt
    15901 caagtttggt cagaaagctc accagatcgg gttcgttggc aatgacctga acgtggggaa
    15961 tcagtgtttc aagggattgt gcaggaagcg agatattata ctggcggcca agttcaagaa
    16021 tggtctgggg acgaatgttg ccatcaaggt ggcgatggat atcagttaat ggcagggtgg
    16081 tatcaatcat ggtcgcactc tttttctgga tataaaagtg cgcaccatta taaaaacaaa
    16141 gcagggtaaa aatccacgat tgcgcaacga ttttttacgt tgcgcaattg gggttagcga
    16201 acagcgcgga tggcgtttat taatccagcc acaccttttt ccagtttcga acgtgggcag
    16261 ccggcattga gacggacaaa accacgacct tcttcaccat aggtataccc cggcatgatc
    16321 gcgacttttt cttgctcgat aagcgctttt tgcaacgcgt tgtcgtcaat attcaacgga
    16381 cgtagatcaa gccaggccag ataagtggat tgcgggatct gccagttgag ttcaggaaac
    16441 gcggcgttca ttttatctga gatatacgtc aggttatctt tcagatagac gcgtaaggca
    16501 tccagccacg gtgcaccttg ctgataggcg gcgatatggg cagttaacgc cagtaccgaa
    16561 ggggaagaaa gcccatcacg tcctttcagt gccgataaat aggcatcacg gctgctgcta
    16621 ttttctataa tcccgtaagc accggtcagc gcgggaatat tgaaactttt cgatcccgat
    16681 gtcagcaacg cccagtctcc ccgcgccaca ttactccagg gaatatgcgg ctgctcgccc
    16741 cagaccatat ccatatggat ttcatcggaa ataacccgca catcatgacg ctcgcacagg
    16801 tcagccatga tctccagctc atcgcacgtc cacactttcc cggtaggatt ctgtgggtta
    16861 cacaggagca taattttaca ttctggtttc gccaaaacgg cttccagctt gcccatatcg
    16921 caaaaccagc cgtcagcctg cttctctaaa gcaacgggca ttactgtgcg ctggttacct
    16981 tcaatggcct tgtaaaatgc gtcataggcg ggcgtgtgga tcaccacgcc ttcacctgtt
    17041 tcagaccact gacgaatcag ttctgaaacc atatagatga cagaagggcc atacaccacc
    17101 gtctgcgtat caattgcggt gtaatgctgg gtggaaaacc agtgggcaat agccgcgaga
    17161 aactcatcgt ttttccagcg gctgtagcca aatacgccgt gcatcaggcg ctgattcagc
    17221 gcctcgataa tgcagggggc agtggcaaaa tccatgtctg aaatcgtgaa cggtaacagg
    17281 tcagcagtgc cgaaacggtc agcgacataa tcccactgtg tacaccatgt gccatgacga
    17341 tccacgacct ttgaaaaatc gaacatatct tatccttatg cctggacagt atgcatcaga
    17401 ccggccattt catcttttac tgactgaact tgtggcccga taacaacctg caggttatgt
    17461 tgattaagtt gtactacgcc aattgcccga ttgtccttca gtgcctgcac attaacaagc
    17521 gacatatctt ccacagacaa acgcagacgg gtaatgcagt tatcgaggct gacaatattg
    17581 tcggcaccgc ctaatgcttc gaggattgca ggaacgttgt aacctgattt acccggcgca
    17641 ccggcaacgg ctttttcgat tgagctggca acttcgctat cgcgccccgg ggttttcaga
    17701 ttgaagcggg tgatagcgaa acggaagatg acgtagtaaa cgacaaacca gattgccgcc
    17761 accactggca ccatgtacca cttggttgac agaccatgca aaataccgaa caccacgaag
    17821 tcgatgatat tgccgtcggt attaccgatg gtgacgccga gcacagacat gacggtgaag
    17881 ccgaggccgg ttaacagcgc gtggatgaca tacagaactg gcgctacgaa caggaacagg
    17941 aattccagcg gttcggtagt gccgccaacg acgcaggcga tcaggccaga aatcagcaga
    18001 cctttaattt tatggcgatt ttccgggcgc gcgcagtgat acatagctaa cgctgcacct
    18061 ggtagaccgc cgagaaacgc aggcatttta ccttgcgaaa ggaaacgcgt gacgctttca
    18121 gaaaaaccgt gagtggtcgg gcaactcaat tgcgcctgga agatggtcag tgcgccgctg
    18181 acggtttgac cgcagacctc ctgcgtgccg cctgcgtcgg taaagcgaat taatgccacc
    18241 agaatgtgat gcagaccaaa cggcaacagc agacgttcac cggtaccaaa cagcatcggt
    18301 ccgaaatcac ccgcgctgtt tatcatatgg cccaagccgc taatacccat ggcgaaaatc
    18361 ggccagacta atggaatcac caggccgaca aggcccatca ccagcgagga gataattggt
    18421 acgaagcgcg taccgccgaa gaatgccagc gcatccggca ggcggatatt atggaaacgc
    18481 tcatgcagca tccagacgat aataccggcg atcaccgcac cgaggatccc ggtatcgatc
    18541 gactggatcc caagaatgct ctggatgtta ttggctttca gaacggcggc atctgtggtt
    18601 ggcagaatgc ctttattggt caaccagaag tttaccgcga ggttcattac cgcataaccg
    18661 acgaagccag cgaatgccgc tacgccttta ttttcgcgtg ccaggcccag cgggatggcg
    18721 atacagaaca tgacaggcag gaaactaaaa gcaaacgagc caatcttact catccaggta
    18781 aagatagcct gcaacacggg gttgcccagg accgggatca gggttatgac atcatggctg
    18841 ctaagagaac taccaatgcc gagcataatg ccgcagaacg acaataatgc cacgggtaac
    18901 atgaaggttt tgcctaactg ctggaagaac tcccacagcg tgactttcgg tgctgttttc
    18961 gctgtcataa aacgactcct cgtagagaac aatctggatt cagtaaattg cgcgagaaga
    19021 taaaacgttt tatcaaattt tagtgagtca taaatcacat tacgcaacga taatagcggg
    19081 tataagataa ataaaaggta aaacgtttta tctgtcacat aatcagggag taggtcatct
    19141 gcatggctac cgccaaaaaa ataaccattc atgatgttgc gctggctgcg ggcgtgtcgg
    19201 taagtaccgt ttcgctggtg cttaatggca aagggcgaat ctctaccgcc acaggagaac
    19261 gcgttaacgc cgccattgaa gagctgggat ttgtgcgcaa tcgacaggcg tcggcgctgc
    19321 gcggcgggca aagcggcgtc attggtttga tcgtccgtga tttatctgcg ccgttttacg
    19381 ccgaattgac ggccggattg acggaagctc tggaagcaca gggacggatg gtttttttgc
    19441 ttcacggcgg taaagacggc gagcagctgg cacagcggtt ttcactgtta ctgaatcagg
    19501 gtgtcgatgg tgtggtaatt gccggggctg caggaagcag cgatgacctg cggcggatgg
    19561 cagaagaaaa agctatcccg gtgattttcg cttcccgtgc cagttatctt gatgatgttg
    19621 atacggttcg cccggacaac atgcaggctg cacagttgtt gacggagcat ctcattcgca
    19681 atgggcatca gcggatcgcc tggctgggag ggcaaagttc ctcattaacc cgtgcagaac
    19741 gggtgggggg ctattgtgca actctactaa aatttggcct gccgtttcac agcgattggg
    19801 tgttggagtg cacttccagc cagaagcaag ccgcggaagc tatcacggcg cttttacgtc
    19861 ataacccgac catcagtgcc gtggtttgct ataacgaaac tattgcgatg ggggcatggt
    19921 ttggtttgct gaaagcaggg cggcaaagcg gggaaagcgg agtcgatcgt tactttgagc
    19981 aacaggtttc gctggcggca tttaccgatg cgacaccaac cacacttgat gatatacctg
    20041 ttacctgggc cagcacgcca gcgcgggaac ttggtatcac acttgcggat cgcatgatgc
    20101 aaaaaatcac ccatgaagag acgcattcac gcaatcttat tattcccgcc cggctcattg
    20161 cggcgaaata atcctctctt tatctgctat acttggtagt gtcctttcct caaggttaat
    20221 gataaaaaat gtggcacaca ggcggtgcct tttattttta ctgaagttca caggaggttt
    20281 atgtgtttaa ttctgacaac ctgagactcg acggaaaatg cgccatcatc acaggtgcgg
    20341 gtgcaggtat tggtaaagaa atcgccatta cattcgcgac agctggcgca tctgtggtgg
    20401 tcagtgatat taacgccgac gcagctaacc atgttgtaga cgaaattcaa caactggatg
    20461 gtcaggcatt tgcctggcgt tgtgatatta cttccgaaca ggaactctct gcactggcag
    20521 actttgccgt cagtaatctg ggtaaagttg atatcctggt taacaacgcc ggtggcggtg
    20581 gtcctaaacc gtctgatatg ccaatggcag attttcgccg tgcttatgaa ctgaatgtgt
    20641 tttctttttt ccatctgtca caacttgttg cgccagaaat ggaaaaaaat ggaggtggcg
    20701 ttattctgac catcacttct atggcggcag aaaataaaaa tataaacatg acttcctatg
    20761 catcatctaa agctgcggcc agtcatctgg tcagaaatat ggcatttgac ctgggtgaaa
    20821 aaaatattcg ggtgaatagc attgcgccgg gggcaatatt aaccgatgcc ctgaaatccg
    20881 ttattacacc a
//
LOCUS       DAJTRB010000073        20714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTRB010000073 DAJTRB010000000
VERSION     DAJTRB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.073"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..579
                     /locus_tag="OO330_002848"
     CDS             <1..579
                     /locus_tag="OO330_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5883650.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO330_002849"
     CDS             597..1235
                     /locus_tag="OO330_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5883651.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO330_002850"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO330_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5883652.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO330_002851"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO330_002851"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5883653.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO330_002852"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO330_002852"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5883654.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO330_002853"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO330_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5883655.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO330_002854"
     CDS             complement(5552..6613)
                     /locus_tag="OO330_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5883656.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO330_002855"
     CDS             complement(6610..8007)
                     /locus_tag="OO330_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5883657.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO330_002856"
     CDS             8163..9161
                     /locus_tag="OO330_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5883658.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO330_002857"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO330_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO330_002858"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO330_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO330_002859"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO330_002859"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5883659.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO330_002860"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO330_002860"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5883660.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO330_002861"
     CDS             complement(14232..14855)
                     /locus_tag="OO330_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5883661.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO330_002862"
     CDS             complement(14852..15274)
                     /locus_tag="OO330_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5883662.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16007)
                     /locus_tag="OO330_002863"
                     /pseudo
     CDS             complement(15296..16007)
                     /locus_tag="OO330_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16025..17077)
                     /locus_tag="OO330_002864"
     CDS             complement(16025..17077)
                     /locus_tag="OO330_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5883663.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17108..17950)
                     /locus_tag="OO330_002865"
     CDS             complement(17108..17950)
                     /locus_tag="OO330_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5883664.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(17937..18818)
                     /locus_tag="OO330_002866"
     CDS             complement(17937..18818)
                     /locus_tag="OO330_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5883665.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18839..20122)
                     /locus_tag="OO330_002867"
                     /pseudo
     CDS             complement(18839..20122)
                     /locus_tag="OO330_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20136..>20714)
                     /gene="ycjM"
                     /locus_tag="OO330_002868"
     CDS             complement(20136..>20714)
                     /gene="ycjM"
                     /locus_tag="OO330_002868"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5883666.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5058 a         5646 c         5033 g         4977 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa agtatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaatgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccacagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc gctataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcgc
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgttc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacga cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatata cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccacccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggcggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg aggtcatcgg cggtaagcgg aaggtaaaca tgccccacgc
    15721 tgccggaacc cccccacttc tgccagcgcc tcgagaatgc gttcgatctg ctttaagcca
    15781 ttaagacgac gctcttcgat aaagtcacca atccagccgt catagccgcc gcaggcggtg
    15841 gtcactggta aaccggtttc tttgatcgcc gctttgactt cttcgatgtt gttgaccagc
    15901 agtttgccgt caatctcaaa accatcgaag cccatctctt tgatataacg aaatctctcg
    15961 cggatatttt ccggaaagaa tgcctgattc tgtgtgccga ttttcatttt ttcattcctt
    16021 tgaattaaaa cgtaacgccc attttgatgc tctgttccgg atgctgatcg acatactgca
    16081 tgtagctttc cgggctgttg gcaaaggtca ctaccgggtc gattaaatct tcgcaattga
    16141 gataaccgtt catcagcagt tcccagcagg tttcttcaat acgcttacgg ctccagcgcg
    16201 gataatccgg gttcggttcg ctgcacgcgc gggagaaaac aattttggcg ttattgaaat
    16261 gcgcttcgcg tccgaggtta aaaccttcag caaatggttt ggcaaacgcg acataggaga
    16321 tggtgccgcc gtaggccagg ccgcgcagcg ccgattgcag cgcgtcggcg taaccgctgg
    16381 tttcgataat cacatcagcc ccctgcttgc cggtcagcgt tttgatctct ttgcctacgt
    16441 cagtgccaat ggggttaagg cagaaatcag caccgtggcg acgggcaata tcacagcgat
    16501 gggcgatagg atcgacgcca attaccaccg atgcgccagc gcgtttcgcc agttggatgg
    16561 caatttgacc gatcgcgcca agccctacca ccacgacaaa atcccctacg cgtacgttgg
    16621 catcgcgcac gccgctcatg gcaaactgcg ccgggtcgta acagacggcg tttttccagg
    16681 agctgccttg cggcattttg cgcagcttgt agttattcac tgcgttaatg atgaccgtct
    16741 cggagagcgg gccgtagccg catacgctgt cgcccaccgc gtagtctgta acgtcgctgc
    16801 cgcactcgat aatgtcgcca accaccatgt tgccaagctg gaatttgcca aactcaatgc
    16861 cgcgcggcgc atctgccgga cgcggggtga acatctgcca ctcgccgtta aagtcttcat
    16921 cgataaacgg gctggcggcg cggaagtcga ccacttccgt tccgtgtttc ggtgcgccga
    16981 aacgcacgcg gatcttcact tcattggcta aaatcgcccg atcttcatac tcaaccagcg
    17041 cagcaacacg cggtgctgtg gctactaact ttttcatgta aaactccttg taaagaaact
    17101 ggccgcttta gcccttcacg ccaccggcgg tcaaaccact cttaataaaa cgttccgaaa
    17161 gcgcatacat aatcaccacc ggcaatgcgg tcaccagcga ggccgccatc atccgtcccc
    17221 agatgtaatc aggcgtactg aacagcgcgt tcaggcccac tggtaaggtg aaattgctgg
    17281 cgctggagag gaaaatcgac gcaaacagat agtcgttcca cgccaccata aagcagtaga
    17341 cgaacaccga tatcagcccg gacatcgcca ggggcacggt gatacggaag atgatttgca
    17401 gacggttgag gccatccatc atcgccgctt cttcgatctc atcagggatg gtgtcgaagt
    17461 aacttttcag cataaacacg gcggtgggca gggtttgcgt caccatggta atgatcagcg
    17521 ccatctcggt gtcgtaaata ccaagcgcgg tgatgatttt gaacagaggc accaccagca
    17581 aaatgccaga gaacatatac accgtgtaaa agctggcgtt gatggtcatc cggcctttaa
    17641 agcgcaggcg ggaaagcgcg taagctccga gaatgccgag aaataccgcc accacggagg
    17701 aaaccaccga caccaccatg ctgttacgga agtagtcgac aaacgggaaa atcatcgggt
    17761 taaaaatgtc gacgtaatgc tccagcgtcc actgttgcgg cagcagcgta ggatgcagcg
    17821 atatcgcctc tttcgcgctt ttgaacgagg tcatcagcat cacaaaaaac ggaaacagcg
    17881 tgatgatgag aaacagcgcc agcccgcagt aaaaaccgat acgactgagc gtgcgtttat
    17941 ttgttgccat tgaggttcac ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg
    18001 aagagcacca ccgagatcgc cgccgctttc cctaagtcat taaaggcaaa ggcggttttg
    18061 tagagataga caccaagaat atcgaccttg gttgtcagca gataaacatc agcgaacatg
    18121 tagaacatcc agatggtacg cagggtcacc accgtcgcca ggaccggcat aattgcgggc
    18181 agcgtgacga tgcgaaaccg ctgccaggca ttagcgccat ccatctctgc cgcttcgtac
    18241 agcgatttgt cgatggtctg caaaatcgcc agaaacgaga taaaggcata cgggaagtag
    18301 cgccagatgg cgaacagcac caccagcaca aagctactgc ccggattatc aaaccacagc
    18361 ggtgcctgct catagagatg cagaagatcg acgccgaggt agttcacaat gccgtagccg
    18421 ttgttgaaca tgtatttcca ggcaaacacc agcgaaatgg acggcgttac gtaggagagg
    18481 atcaccagcg aacgcgcggt tttgcgcagg cggaactcgc ggttgaaaaa catcgccacg
    18541 gccagcccga gcacggtgct gcccgccacc accagcgccg tataccagac cgtcatccac
    18601 agcgagtgcc agaagccggg atcggagaga atgcgcacat agttactcac cccaacaaac
    18661 gttgactcga tgttgggatt gagcggcaga cgtaaaaaac tgatttcgat attcgacacc
    18721 atcggccacg ccaccagacc gcccagcagt aataagctgg gcgcgagaag cagcagcgca
    18781 aacggcatat cggaacgacc tgaaaaaagc ctgttcattg cggattcctg tcctgggttt
    18841 agtgctgttc gatcagttca tccagctttt tctggctcgc ttgcagcgtg ccagggagat
    18901 cggctttacc cacggtgacg ttatgcacca tggagctcac cacaccagaa cccgtcacat
    18961 cgcccatgcg ggtaaagttt ttatccccta ctgcgccaaa gacctgaata tttggcagtt
    19021 caccgattag ctgattcggt agttcaccca gcgccttaat aacgtcgttg tctttccagg
    19081 tggcggtagt gacaaccgct ttattcaccg gcagcgcagc acctggcgac atcatcaccc
    19141 agtcggcaat gttgtccgcc tgctccataa aggtgacaaa tttttctgct gcttcggtct
    19201 tttgtccggc ggtaatggtc agcgaggtca acatgccgta gaccgcagag tttttctcgg
    19261 ttggcaccac gaaaccgacg tttttcgggt cgccttcttt aatcacagcc ggaaggatat
    19321 aggtggagta aatcgccatc ggcgcggtgc cgttcataaa ggcgtctttc acttccatga
    19381 tgtcgttaga acccggcatg gtgttggcag caaggtcgcg gtaataggtc agcgcctgca
    19441 tcatctctgg tgtatcaagg gtgattttgc cttcggcgtt aaagacgttg gcctagttgg
    19501 ataacgcaaa ctgggagaag gattgttccg tcagcacgct ttctgccgta ggcagcgcaa
    19561 taccgtattt tttattcgcc gggtcattca gtttctgtgc aacgtccagc agctgttgcc
    19621 agtttttcgg ctcctccagc cccgcttttg ccagcacatc tttgcgatac cagatgccgc
    19681 caatccaggc gctgacagga acaccggtcc atgcgctacc atcttcggta cgcacaatac
    19741 gcagtacgcc atcgtaaaac gcgccttcac caacgttgct gatgaccgtg gcaaccgctt
    19801 tgcgatcgat aagctgctct ttatccataa ctttggcgta gtcatggctg gtttcgatca
    19861 cttccggcag cgagccgcta cgtgaaaggg tgatgacttt ggtgttatga gcgtcttctt
    19921 ccacgggcac ttgtttaacg gtgatacctg ggttttcttt ttcaaaacgg gcaatcaatt
    19981 tgctgataac tgcctgacgc tcctgctcca ccgaagaatg cataaattcg atggatacat
    20041 gggttttatt ttcttcttta cagcctgaaa tcagggcgca ggaaaccagt gctgataaca
    20101 gcacgatttt tgatttaatc atgagatgtt cctttttatt taatccacat aacctgccag
    20161 gggcgcagcg ttatttcatt acccaatata tcaacttcac taatcagatc ccgaccatgc
    20221 agatcagtaa tatttacatg ctgaatattt ttactgacat taaacaatcc agtcagacaa
    20281 ttaccatcag cgttacttct ttgaatacgc attacggatg aattaaccgt atcgatggta
    20341 aaattattat ccggatgaaa ctcgttatgg ctgcgacgaa gtgtaattaa acgcgacaac
    20401 tcatgatata ccgcatgcct taatgtagct tcatcgttca gttctcgtgt tatctcttca
    20461 ctgtgatatt ttttacggtt aatcgcacgg ttatatccga gtttttcgac acctgcgtaa
    20521 tcattacgcg agccaagaat actttgaata tatatcgcgg gtacaccggg gaaacttaac
    20581 aaaatcgcat gggcaaggat aaacctggtg caacgttctt catcgctact ctcgcggcgg
    20641 cttaacgcat tcatataggt gacattcatc tcatagggac tgcgcgtgcc gtcgggatta
    20701 tttttccagt ttac
//
LOCUS       DAJTRB010000074        20708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTRB010000074 DAJTRB010000000
VERSION     DAJTRB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.074"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO330_002869"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO330_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5883667.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO330_002870"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO330_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5883668.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO330_002871"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO330_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5883669.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO330_002872"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO330_002872"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5883670.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO330_002873"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO330_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5883671.1"
                     /translation="MLFTLKKVIGNMLLLLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO330_002874"
     CDS             complement(8217..9110)
                     /locus_tag="OO330_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5883672.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO330_002875"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO330_002875"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5883673.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO330_002876"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO330_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5883674.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO330_002877"
     CDS             complement(10241..11473)
                     /locus_tag="OO330_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5883675.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO330_002878"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO330_002878"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5883676.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO330_002879"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO330_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5883677.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OO330_002880"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OO330_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO330_002881"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO330_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5883678.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO330_002882"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO330_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5883679.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO330_002883"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO330_002883"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5883680.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO330_002884"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO330_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5883681.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4982 a         5725 c         5031 g         4970 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgctacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 aggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggcc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaat
//
LOCUS       DAJTRB010000075        20700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTRB010000075 DAJTRB010000000
VERSION     DAJTRB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.075"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            82..351
                     /locus_tag="OO330_002885"
     CDS             82..351
                     /locus_tag="OO330_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5883682.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OO330_002886"
     CDS             345..710
                     /locus_tag="OO330_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5883683.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO330_002887"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO330_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5883684.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OO330_002888"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OO330_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5883685.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OO330_002889"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OO330_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5883686.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OO330_002890"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OO330_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5883687.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO330_002891"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO330_002891"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5883688.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVSNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO330_002892"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO330_002892"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5883689.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OO330_002893"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OO330_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO330_002894"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO330_002894"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5883690.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO330_002895"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO330_002895"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5883691.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14858..16456
                     /gene="rtcR"
                     /locus_tag="OO330_002896"
     CDS             14858..16456
                     /gene="rtcR"
                     /locus_tag="OO330_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5883692.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16438..17196)
                     /locus_tag="OO330_002897"
     CDS             complement(16438..17196)
                     /locus_tag="OO330_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5883693.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17213..18043)
                     /gene="glpG"
                     /locus_tag="OO330_002898"
     CDS             complement(17213..18043)
                     /gene="glpG"
                     /locus_tag="OO330_002898"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5883694.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18088..18414)
                     /gene="glpE"
                     /locus_tag="OO330_002899"
     CDS             complement(18088..18414)
                     /gene="glpE"
                     /locus_tag="OO330_002899"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5883695.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18604..20109
                     /gene="glpD"
                     /locus_tag="OO330_002900"
     CDS             18604..20109
                     /gene="glpD"
                     /locus_tag="OO330_002900"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5883696.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20378..20608)
                     /locus_tag="OO330_002901"
                     /pseudo
     CDS             complement(20378..20608)
                     /locus_tag="OO330_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4891 a         5693 c         5218 g         4898 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcgaga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcgcttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaattttt taatttattg
    14761 tttctaaaaa gattatatct ttatgtccgt aaacggagat ttcccgcaaa gccgatttac
    14821 cgataatgaa atatcgcctt ttataaggat atctaagatg cgtaaaacag tggcttttgg
    14881 ctttgtcggt accgtgctgg attatgccgg acgcggcagt cagcgctggt caaaatggcg
    14941 tccgtcactc tgtatatacc agcaagaatc gttggtcatc gatcgactgg aattattgca
    15001 cgatgcccgc tcgcgctcgc tatttgaaac gcttaaacgc gatatcgcca gcgtttcgcc
    15061 agaaacagaa gtggtgggcg ttgagattga actgcataac ccgtgggatt tcgaagaggt
    15121 ttacgcctgc ctgcatgatt tcgcccgtgg ttacgagttt cagccagaaa aagaagacta
    15181 tttaattcac atcaccaccg gcacccacgt cgcgcagatt tgctggtttc tgctggcgga
    15241 agcgcgttat ctacccgccc ggttgataca atcttcaact ccgcgcaaaa aagaacagcc
    15301 gcgcggccca ggtgaagtga cgattatcga tctcgattta agccgttata acgccatcgc
    15361 cagccgcttt gccgaggaac ggcagcaaac gctcgatttc ctcaaatccg gtattgccac
    15421 gcgtaacccc cacttcaacc gcatgattga gcagatcgaa aaagtggcga ttaaatcccg
    15481 cgcgccgatt ctgcttaacg gcccgaccgg cgcaggtaag tcatttctgg cgcgacgcat
    15541 cttcgagtta aaacaggcac ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc
    15601 caccctgcgc ggcgataccg cgatgtcgac gctgtttggt catgtgaaag gcgcgtttac
    15661 cggggcgcgg gaatctcgtg aaggattatt acgcagcgcc aacggcggaa tgttgtttct
    15721 tgatgagatt ggcgaactgg gcgcagacga acaggcaatg ctgctgaaag ctattgaaga
    15781 gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc agtgattttc agcttatcgc
    15841 cggaacggtg cgcgatttgc gccagctggt tgccgaaggc aaattccgcg aagatctgta
    15901 cgcgcggatc aatctctgga ccttcaccct gccgggtcta cgccagcgcc aggaagatat
    15961 tgaaccgaac ctggattatg aagtggagcg ccacgccaca ctcactggtg acagcgtgcg
    16021 ttttaacacc gaagcgcggc gtgcctggct ggcctttgca acatctcccc aggcaacatg
    16081 gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg atggccacct ttgccactag
    16141 cggacgcatc actctggacg tggtggaaga tgagataaac cgtctgcgct ataactggca
    16201 ggagagccgc ccctccgcgc ttacggcgtt gctgggcgca gaggcagaaa acatcgatct
    16261 cttcgaccgt atgcaactgg aacacgttat cgctatctgc cgccaggcaa agtcgctttc
    16321 tgctgccgga cgacagcttt ttgatgtttc gcgccagggc aaagccagcg tcaatgacgc
    16381 ggatcggcta cgcaaatacc tggcgcgttt tggtctgacg tgggaagccg tgcaggatca
    16441 gcacagctcc agttgaatat ggtggtccgt cagcacctgc atcacgctta ctggcggcgg
    16501 ggcgtcggtg tagacggcat caaccatgct gatgctcccc atattgacca tcgcattacg
    16561 gccaaatttt gaatggtcaa ccaccagcat aacgtggcgc gagttctcaa taatggcgcg
    16621 cttggtacga acttcgtggt aatcgaactc cagcagcgag ccgtcgctgt cgatgccgct
    16681 gatccccaga atgccgaaat caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc
    16741 aatgatcccg ccatcgcggc tacgtaattc gccaccggcg agaataatgc ggaaatcttc
    16801 ttttaccatc aacgtgttag caacgttgag attgttggtg acaatgcgca aattgctgtg
    16861 attgagcagc gcgtgcgcca ccgcttccgg cgtggtgccg atatcgataa acagcgtcga
    16921 gccattgggg atttgctccg ccactttgcg ggcgatgcgc tctttttctt cggtctgggt
    16981 ggctttacga tcgtgccacg gcgtgttaac cgaactggaa ggcagcgccg cgccgccatg
    17041 atggcgcagg atcaggtttt gctctgccag ctcgttgagg tcgcggcgaa tagtctgcgg
    17101 gctgacggag aagtgctcca ccagttcctc ggtactgaca taaccctgct gtttaaccag
    17161 ttcgataata ccgttgtgac gttgtgtttg tttcatttat aaatccctgg aattattttc
    17221 gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa acccacggct aatccggcga
    17281 tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa ccatccggcg acaatccaga
    17341 tcagcgcaaa gataattaac ccacgttgca ggtaaatgcc actttgcgga tcgcgttcgc
    17401 cacgtagcca gacgtagccc atcagcgcat acaccacgcc agaaagcccg ccaaaccacg
    17461 gcccgctgaa tttttgctgc atatagccgc ttaacagggc gctgataagc gtaatgacaa
    17521 ttagcttacc gctaccgagg cgtttttcca ccgcgccgcc gagataccac caccaaagca
    17581 ggttaaagag gatatgcatc agcgagaagt gcattaacgc gtgggtgaag taacgccaga
    17641 actcaaattt cagtgttgga tcgaatggcc aggccagcca taacatcact tcctgatcgc
    17701 cgagaatttg catggcaata aacaccacca cgcaggcgat catcatcacc caggtcaccg
    17761 gacctgcgcg ttcacgcaag gcagcaaaga aaggataacg gcgataatgc aggccactgc
    17821 cggtatggcc tgccagccag ctggccgcca gataacgcgg atctgccggg ttttcgagaa
    17881 aacgcgccag ctccgcccgc acgcgctcgg cctgggactc atccgccagc cagacatcgc
    17941 tttggttatg ttgttgaatc gtgaggataa caccctgcgt cgccatgtaa tcaacaaacg
    18001 cctgcgccac gcgggggtta gcaaaagagg taatcatcaa catcgttgct gtcgcttatt
    18061 ccacacaaaa ggggacagta taaagcgtta cgcgccgtac gccacctctg cgggaaactg
    18121 tcgctgccag gcttcaaagc cgccgtcaat gctatagacc acatcgtagc cctgttgcag
    18181 cagatactgc gccgcccctt tgctgctatt gccgtgataa cacatcacca tcaccggagt
    18241 gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg
    18301 caccgcatgc cccatagcga aactctgtgg atcgcgaata tcgaccagca ccgcctcttt
    18361 ttcctgcaac ttctggtgcg cgtcggcaac gttaatacat tcgaactgat ccatgcgtct
    18421 ctctttcttt acaaacaagt gggcaaattt accgcacagt ttacgtcgaa gcggcagata
    18481 aacgccataa tgttatacat atcactctaa aatgtttttt caatgttacc caaagcgcga
    18541 ttctttgcta atatgttcga taacgaacat ttatgagctt taacgaaagt gaatgagggc
    18601 agcatggaaa ccaaagatct gattgtgata ggtggcggca tcaatggtgc tggtatcgcg
    18661 gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg aggcgcagga tctcgcttgc
    18721 gcgacctctt ccgccagttc aaaactcatt cacggtggcc tgcgctacct tgagcactat
    18781 gaattccgcc tagtcagcga ggcgctggct gaacgtgaag tgctgctgaa aatggctccg
    18841 catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg
    18901 atgattcgca ttggtctgtt tatgtacgat catctgggta aacgcaccag cctgccggga
    18961 tcaactggtt tgcgttttgg cgcaaattca gtgttaaaac cggaaattaa gcgcggattc
    19021 gaatattctg actgttgggt agacgacgcc cgtctggtac tcgccaacgc ccagatggtg
    19081 gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac
    19141 ggcctgtgga ttgtggaagc ggaagatatc gataccggca aaaaatatag ctggcaagcg
    19201 cgcggcctgg ttaacgccac cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat
    19261 ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc atattgtggt gccgcgcgtg
    19321 catacccaaa agcaggccta cattctgcaa aacgaagata aacgcattgt gttcgtgatc
    19381 ccgtggatgg acgagttttc catcatcggc accaccgatg tggaatacaa aggtgatccg
    19441 aaagcagtga agattgaaga gagtgaaatc aattacctgc tgaaagtgta taacgcgcac
    19501 tttaaaaagc agctaagccg tgacgatatc gtctggacct actccggtgt gcgtccgctg
    19561 tgtgatgatg agtccgactc gccgcaagcc attacccgtg attacaccct tgatatccat
    19621 gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg gtaagctgac cacctaccga
    19681 aaactggcag aacatgcgct ggaaaaactg acgccgtatt atcagggtat tggcccggcg
    19741 tggacgaaag agagcgtgct gccgggtggt gccattgaag gcgaccgcga cgattacgcc
    19801 gctcgcctgc gccgccgtta cccgttcctg actgaatcgc tggcacgtca ctacacgcgc
    19861 acttacggca gcaacagcga gctgctgctc ggcaatgcgg gaacggtaag cgatctcggg
    19921 gaagatttcg gtcatgagtt ctacgaagcg gaactgaaat acctggtgga tcacgaatgg
    19981 gtccgccgca ccgacgatgc cctgtggcgt cgcacaaaac aaggcatgtg gctgaatgcg
    20041 gatcaacaat ctcgtgtgag tcagtggctg gtggaatata cgcagcagaa gttgtcgctg
    20101 gcgtcgtaaa ttaacgtaag gtgatcaggt cagatttcaa tctggcctga gactgatgac
    20161 aaacacaaaa ctgcctgatg cgctacgctt atcaggccta cgtggtttat gcaatatatt
    20221 gaatttgcat ggtcttgtag gccagataag acgttcacgt cgcatccggc atgaacaaag
    20281 cgcactttgt caaaaatcta acctactttt taattcaggg aattaccgca aagcccacgc
    20341 catcataccc aacgtaacaa aactcaggca cgttccccac tcgccccgag aaaaatagca
    20401 ttaatgcgcc cagcgtcagc ataaaaatat tgagcagaag gtaaattgtt ggcgtaaaga
    20461 gtaaattgca aacctcagga atagcggcaa gcccagcccc cagcatggca ataaccgctg
    20521 caaaaaattg cgtcagtatt gggatgccga aaaaagtcat taccagcgag gtcaaaatcc
    20581 attttctatt ttgcattatt ctttccattc tttctgaatg gcgaaattat actccccgag
    20641 tccccttgcc ccttctggac acttttccga aatgatggcg aaaaaaacgg gaccctttgg
//
LOCUS       DAJTRB010000076        20612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTRB010000076 DAJTRB010000000
VERSION     DAJTRB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.076"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            96..395
                     /locus_tag="OO330_002902"
     CDS             96..395
                     /locus_tag="OO330_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5883697.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="OO330_002903"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="OO330_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5883698.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO330_002904"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO330_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5883699.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGLNPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="OO330_002905"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="OO330_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5883700.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="OO330_002906"
     CDS             complement(2534..2878)
                     /locus_tag="OO330_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5883701.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="OO330_002907"
     CDS             3030..3260
                     /locus_tag="OO330_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883702.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="OO330_002908"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="OO330_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5883703.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="OO330_002909"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="OO330_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5883704.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="OO330_002910"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="OO330_002910"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5883705.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="OO330_002911"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="OO330_002911"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5883706.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPVEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="OO330_002912"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="OO330_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5883707.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALYALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="OO330_002913"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="OO330_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5883708.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="OO330_002914"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="OO330_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5883709.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="OO330_002915"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="OO330_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO330_002916"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO330_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5883710.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="OO330_002917"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="OO330_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5883711.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="OO330_002918"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="OO330_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5883712.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="OO330_002919"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="OO330_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="OO330_002920"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="OO330_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5883713.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO330_002921"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO330_002921"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5883714.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO330_002922"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO330_002922"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5883715.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="OO330_002923"
     CDS             complement(19399..19827)
                     /locus_tag="OO330_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5883716.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO330_002924"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO330_002924"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5883717.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4971 a         5274 c         5149 g         5218 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taatcggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aagtccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc tgaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat gcaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcacgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatatcgg catgtaaaaa
     1861 tccacaatgt acaaaaaaag agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt cactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga
     1981 tgacaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtggttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgtagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaaggaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agccctttat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gtcttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaatgac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg ctccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg at
//
LOCUS       DAJTRB010000077        20570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTRB010000077 DAJTRB010000000
VERSION     DAJTRB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.077"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            409..1239
                     /locus_tag="OO330_002925"
     CDS             409..1239
                     /locus_tag="OO330_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5883718.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPSTAEIHSKMGYNVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO330_002926"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO330_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5883719.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO330_002927"
     CDS             complement(2368..3828)
                     /locus_tag="OO330_002927"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5883720.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO330_002928"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO330_002928"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5883721.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO330_002929"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO330_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5883722.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO330_002930"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO330_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5883723.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO330_002931"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO330_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5883724.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO330_002932"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO330_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5883725.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OO330_002933"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OO330_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5883726.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OO330_002934"
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OO330_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5883727.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYSPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12470..13009)
                     /locus_tag="OO330_002935"
     CDS             complement(12470..13009)
                     /locus_tag="OO330_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5883728.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OO330_002936"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OO330_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5883729.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="OO330_002937"
     CDS             complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="OO330_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5883730.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="OO330_002938"
                     /pseudo
     CDS             complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="OO330_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17233..17949
                     /gene="nanC"
                     /locus_tag="OO330_002939"
     CDS             17233..17949
                     /gene="nanC"
                     /locus_tag="OO330_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5883731.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17969..19075
                     /gene="nanM"
                     /locus_tag="OO330_002940"
     CDS             17969..19075
                     /gene="nanM"
                     /locus_tag="OO330_002940"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5883732.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19140..20120
                     /locus_tag="OO330_002941"
     CDS             19140..20120
                     /locus_tag="OO330_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5883733.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5475 a         4910 c         4673 g         5512 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctagtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaaaatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggtgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcctggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggct atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 tttgtcgttt tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg
    14041 acagtttggc cccaattgtc ttgtgtttat ttgtttttta actttattat caataagtta
    14101 aatcaaacct cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg
    14161 gcaaaacgag cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgatgcccg
    14221 agataatcct gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca
    14281 tgccttaaca tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga
    14341 ataatgcgat aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg
    14401 tcggtccggt cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca
    14461 cgctcatcaa aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt
    14521 attcgacctt cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc
    14581 atcccatgcc gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa
    14641 caaaccgcct gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca
    14701 atagtagtac tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt
    14761 caaaattagc ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa
    14821 ttacattaaa catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg
    14881 atttgattca atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa
    14941 gaaaaaagtg tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt
    15001 atttattttg aagaacataa accattagat tttgtatggg tatggttaac ttaatgttta
    15061 ttatgataat taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt
    15121 gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat
    15181 aaaacagcat gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca
    15241 ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata atcctagata
    15301 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc
    15361 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
    15421 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
    15481 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
    15541 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
    15601 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
    15661 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
    15721 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
    15781 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
    15841 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
    15901 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
    15961 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
    16021 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
    16081 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc
    16141 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
    16201 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
    16261 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
    16321 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
    16381 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
    16441 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
    16501 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta
    16561 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
    16621 aaataaatta tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
    16681 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
    16741 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
    16801 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
    16861 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
    16921 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
    16981 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
    17041 atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac
    17101 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
    17161 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
    17221 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
    17281 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
    17341 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
    17401 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
    17461 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
    17521 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
    17581 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
    17641 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
    17701 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
    17761 cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta
    17821 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
    17881 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
    17941 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
    18001 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
    18061 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
    18121 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
    18181 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
    18241 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
    18301 ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca
    18361 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
    18421 caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
    18481 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
    18541 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
    18601 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
    18661 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
    18721 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
    18781 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
    18841 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
    18901 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
    18961 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
    19021 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
    19081 cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata
    19141 tgaacgcaat aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg
    19201 ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa
    19261 ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt cactttaacg
    19321 acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc
    19381 ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac
    19441 ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg
    19501 gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc
    19561 atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc
    19621 gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg
    19681 aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg
    19741 aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc
    19801 cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag
    19861 ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc
    19921 aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg
    19981 gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc
    20041 atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc
    20101 agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc
    20161 agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa
    20221 cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa
    20281 ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt
    20341 atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt
    20401 cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag
    20461 acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg
    20521 cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcattat
//
LOCUS       DAJTRB010000078        20568 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTRB010000078 DAJTRB010000000
VERSION     DAJTRB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.078"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            105..257
                     /gene="hokA"
                     /locus_tag="OO330_002942"
     CDS             105..257
                     /gene="hokA"
                     /locus_tag="OO330_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5883734.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(444..656)
                     /gene="cspA"
                     /locus_tag="OO330_002943"
     CDS             complement(444..656)
                     /gene="cspA"
                     /locus_tag="OO330_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5883735.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(937..1227)
                     /locus_tag="OO330_002944"
                     /pseudo
     CDS             complement(937..1227)
                     /locus_tag="OO330_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1661..2371
                     /locus_tag="OO330_002945"
     CDS             1661..2371
                     /locus_tag="OO330_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5883736.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="OO330_002946"
     CDS             complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="OO330_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5883737.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3499..4149)
                     /locus_tag="OO330_002947"
     CDS             complement(3499..4149)
                     /locus_tag="OO330_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5883738.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4312..6645
                     /gene="bisC"
                     /locus_tag="OO330_002948"
     CDS             4312..6645
                     /gene="bisC"
                     /locus_tag="OO330_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5883739.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6614..7054)
                     /locus_tag="OO330_002949"
     CDS             complement(6614..7054)
                     /locus_tag="OO330_002949"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5883740.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7051..7614)
                     /gene="tag"
                     /locus_tag="OO330_002950"
     CDS             complement(7051..7614)
                     /gene="tag"
                     /locus_tag="OO330_002950"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5883741.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7772..8470
                     /locus_tag="OO330_002951"
     CDS             7772..8470
                     /locus_tag="OO330_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5883742.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8699..9907
                     /locus_tag="OO330_002952"
     CDS             8699..9907
                     /locus_tag="OO330_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5883743.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10231..11922
                     /gene="eptB"
                     /locus_tag="OO330_002953"
     CDS             10231..11922
                     /gene="eptB"
                     /locus_tag="OO330_002953"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5883744.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12014..12090
                     /locus_tag="OO330_002954"
     tRNA            12014..12090
                     /locus_tag="OO330_002954"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12048..12050,aa:Pro,seq:cgg)
     gene            12834..14441
                     /gene="dppA"
                     /locus_tag="OO330_002955"
     CDS             12834..14441
                     /gene="dppA"
                     /locus_tag="OO330_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5883745.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTLHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14749..15768
                     /gene="dppB"
                     /locus_tag="OO330_002956"
     CDS             14749..15768
                     /gene="dppB"
                     /locus_tag="OO330_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5883746.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15778..16680
                     /gene="dppC"
                     /locus_tag="OO330_002957"
     CDS             15778..16680
                     /gene="dppC"
                     /locus_tag="OO330_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5883747.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16691..17674
                     /gene="dppD"
                     /locus_tag="OO330_002958"
     CDS             16691..17674
                     /gene="dppD"
                     /locus_tag="OO330_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5883748.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17671..18675
                     /gene="dppF"
                     /locus_tag="OO330_002959"
     CDS             17671..18675
                     /gene="dppF"
                     /locus_tag="OO330_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5883749.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18705..19976)
                     /locus_tag="OO330_002960"
     CDS             complement(18705..19976)
                     /locus_tag="OO330_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5883750.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20452..20559
                     /locus_tag="OO330_002961"
     CDS             20452..20559
                     /locus_tag="OO330_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5883751.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4929 a         5088 c         5408 g         5143 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacaa agtgaggcta ccctatgcct gaacaccata
       61 aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata
      121 gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag
      181 attcactgtg tgaattgcat attaaacagg agagttatga gctggcggcg tttttagcct
      241 acaaattgaa agagtaagag tcttcggcgg gaaattattc ccgccttact tacggcgttg
      301 cgcattctca ttgcacccat atttattctt cacaaaaata ataatagatt ttattacgcg
      361 atcgattatt tatttcctga aaaaaaataa aaaaatcccc gccaaatggc agggatctta
      421 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      481 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      541 ttctggatag cagagaagtg tacgaacaca tctttagagc catcgtcagg agtgatgaag
      601 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      661 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      721 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      781 actacactgc tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cactcattgg
      841 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      901 atcaggaccg taaacagcaa taaagtggat aaaagtctat ttcatcaact gcttacttaa
      961 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
     1021 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
     1081 gatttttaat cctgtccctt ttcataactg ctcaatttcg gtacaggacg ttgttctgtg
     1141 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
     1201 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
     1261 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag
     1321 ggcatgctct ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa
     1381 cgatctgata aatgccgata tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc
     1441 taaccttctg cgaaggcgta aatctctggc tacaaaaaag gcaacggcac tatcattttt
     1501 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1561 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1621 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1681 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1741 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcaaaata cagtgatgcg
     1801 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1861 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1921 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     1981 aagcggtccg ctgcgtgaaa tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa
     2041 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc
     2101 ggtatttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     2161 gattccggcg gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca
     2221 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     2281 aagtgaatat aacaaactga ttgcgccatt accggcacaa catcaggcgt ttaatcaggc
     2341 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2401 ttacttcacg cagtcgcagc ttagtccgcg acgtgaggat tcacacagtt cttctcaacc
     2461 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2521 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2581 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct
     2641 ttctgcaatg ctgcgatcag tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg
     2701 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2761 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg
     2821 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2881 atgggcatgt tgaagccaaa gtgcacacgt tgtgccagcg ccatgccgat ccgtcccatc
     2941 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     3001 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     3061 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     3121 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     3181 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatctac attctcgttt
     3241 gaacccagta aaccttcagc ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg
     3301 ctgaggtttg ccacctggtg aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca
     3361 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3421 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3481 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3541 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3601 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgctg
     3661 ctgggagaga cgcatgttca ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt
     3721 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3781 tgccggtttc aggggagcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3841 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3901 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     3961 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     4021 gcccacgaga gagcccagac ctgcgccgat agcagatttg cctgcttcgc gttcgccggt
     4081 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaagtaa
     4141 gataaacacg tttcttcatt gttaatcctt aataaccttt ttattctttg tcacgggttc
     4201 cgtggcggga gattatgccg cgtgaacatg aagattattc ctgggaatac tcggaaattt
     4261 gtaagtaata tttaactgct caatacatct aacctttcag gagtcttcgg tttggccaac
     4321 tcatcctcac gatattccgt tctgactgcc gcccactggg ggcccatgct ggttgaaacc
     4381 gacggcgaaa ccgtgtttag ctcgcgtggc gcgttagcca caggaatgga aaactccttg
     4441 cagagcgcgg ttcgcgacca ggttcacagc aatacgcggg tacgatttcc aatggtgcgc
     4501 aaaggctttc tcgcgtcacc ggaaaacccg caaggcattc gtgggcagga tgaatttgtt
     4561 cgcgtgagtt gggatgaggc gctggatctt attcaccaac aacataaacg cattcgtgag
     4621 gcttatggtc cggcatcgat ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg
     4681 cataaggcct cgacattatt acaacgctat atggcgctgg caggcggtta taccgggcat
     4741 ctgggggatt attcgaccgg tgcggcacag gcgatcatgc cgtatgtcgt aggtggcagc
     4801 gaagtttatc aacagcaaac cagttggccg ctggtgctgg aacatagcga tgtcgtggtg
     4861 ttgtggagtg ctaacccact caatacgctg aaaattgcgt ggaatgcatc cgatgagcag
     4921 gggctttctt acttttccgc actgcgtgac agtgggaaaa agctgatctg cattgatcca
     4981 atgcgatcgg aaaccgtcga tttctttggc gataaaatgg agtgggtggc accgcacatg
     5041 ggcaccgatg ttgcgctgat gctggggatc gcccatacgc tggtggaaaa tggttggcac
     5101 gacgaagcgt ttctggcgcg ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg
     5161 ggcgagagtg acggaatagc gaaaaacgcc gaatgggcgg cagagatttg tggtgttggc
     5221 gcagcgaaaa tccgcgagct ggcggctatt ttccaccaaa ataccaccat gctgatggca
     5281 ggctggggaa tgcagcgcca acagtttggt gagcaaaaac actggatgat cgtcacgctg
     5341 gcagcaatgt tggggcaaat cggcacaccc ggcggcggtt ttggtctttc ttaccatttt
     5401 gccaatggtg gtaaccccac gcggcgttct gcggtgctct cttccatgca gggcagcttg
     5461 ccgggtggct gcgatgcggt ggataaaatc cctgttgccc gcattgttga agcactggaa
     5521 aaccctggtg gcgcatatca acacaacggt atgaaccgac atttcccgga tattcgtttt
     5581 atctggtggg cgggcggtgc caactttact catcatcagg ataccaatcg tctgatccgt
     5641 gcctggcaaa aaccggagct ggtggtgatc tctgaatgct tctggacggc ggcggcgaaa
     5701 cacgcggata ttgttctgcc tgcgactacc tcttttgagc gtaatgatct caccatgacc
     5761 ggtgattaca gtaatcagca tctggtgccg atgaagcaag tggtgccgcc acgctatgaa
     5821 gcgcgtaatg attttgatgt ttttgccgag ttgagtgaac gttgggagaa gggcggctat
     5881 gcgcggttta cggaaggaaa aagtcagctg caatggctgg aaacgtttta taacgttgcc
     5941 cggcagcgcg gggcaagcca gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc
     6001 aaccagttaa ttgagatgcc ggaaaacccg gacagcgagc ggtttattcg cttcgctgat
     6061 ttttgccgcg atccgctggc gcatccgtta aaaaccgcca gcggcaagat tgaaatcttc
     6121 tcacagcgta ttgccgatta cggttacccg gattgccctg ggcatccaat gtggctggag
     6181 ccggacgaat ggcagggcaa tgccgaacca gaacagttgc aggtactttc tgcccatccg
     6241 gcgcaccgcc tgcacagcca gctgaattac agttctctgc gcgaattgta cgcggtagca
     6301 aaccgtgagc ctgtcaccat tcatcctgac gatgctcagg cgcgcggcat aaccgaaggg
     6361 gatatggtgc gggtgtggaa ctcccgtggg cagatccttg ccggagcggt cattagcgag
     6421 ggaattaaac ctggcgtgat ttgcattcac gaaggggcat ggccggatct ggatttaacc
     6481 gctgacggta tttgtaaaaa cggcgcagtg aacgtgctga ccaaagatct ccccagctcg
     6541 cggctgggga atggctgtgc gggtaatacg acgctggcat ggctggaaaa atacaacggt
     6601 ccggaactga cacttacagc gtttgaacca ccggccagct cataatccag gtgggtagtt
     6661 gggtttcatc ctgccatgcg caatcgacaa tgtgaaaacc ctgcgcacgg taaaaatcta
     6721 tcgccggttg atttttttga taaacctcca gcatcagatg gggatagcgc tgctgcacat
     6781 actgcatcag cgccttacca ataccgcgtc tgacggcctt cggtgcgaca aacattgctg
     6841 ccagaaatcg gccttccata atgctgacaa aaccgagaag cttaccgtcc tcttcccaga
     6901 cccagttttg cgcgttggca agataggcat cccgcaccag cggaatgcag tcacgccagt
     6961 aattcgcttt tataaaggga tgcccccagg ttgtactttc cagccacagt tcgaggatcg
     7021 cggggagttc tgaacgttgc gcttcccgaa tcatggttta tttcccagat agcaacagca
     7081 gccaaccaca tgatcattca ccagcccaca tgcctgcata aaggagtaac agattgtggt
     7141 gccgacaaac ttaaaaccac gttttttcag tgccttagat agggcgtcgg aggcggacgt
     7201 agatgtggga atttcgctca acgttgtggc ttgtgtcacc tgtggctgat gatttacaaa
     7261 cgaccagaca aagtcgggaa acggttcgcc gttctgttcc atttgcaggt acgcccgcgc
     7321 attaccaata attgcctgaa ttttccctcg atggcggata atcccggcgt cctgtaccag
     7381 tctttcgaca tcctcttcct gcattgctgc gaccttcacc ggatcgaact gatgaaagca
     7441 ggcgcgatag ttttcgcgtt ttttgaggac ggtgatccac gataacccag cctgctgccc
     7501 ttcaaagcag atcatttcga acagtttttt actgtcagtt tcaggcacgc cccactcatt
     7561 atcatggtag gcaatataaa gcgggccctg actcacccag ccgcaacgtt ccatactttc
     7621 cctcgctatg tacccgatcg taaaaatttc agccgctttt cttgacgcat cggtgaaaaa
     7681 gtcaatattt aatacagggc tactatgccc gatatccttc atacaatgac aataatattg
     7741 ccgaattcgg ctcttccctg gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg
     7801 gcagctaagc ctgctggcga gcgtggtaat cagtgccttt tttctcaaca cagcttacgc
     7861 ctggcaacaa gaatatatcg ttgatacgca acccggacat tccacagagc gttacacctg
     7921 ggatagtgat catcaacctg attacaacga tattttgtcg caacgtattc aaagtagcca
     7981 aagggcgctg ggactggaag tcaatctggc ggaagaaact cctgtggatg tgaccagcag
     8041 tatgagtatg ggctggaatt ttcctttata tgaacaggtt acaaccggcc cggtcgcggc
     8101 attacattac gatggcacaa ccacctcgat gtataacgag tttggcgaca gtactaccac
     8161 gctgaccgat ccgttatggc atgccagcgt gagtagctta ggctggcgtg ttgactcccg
     8221 gcttggcgat ctccgaccct gggcgcaaat cagctataac cagcaatttg gtgagaatat
     8281 ctggaaggcg caatcaggcc tgagccggat gacggcgaca aaccagaacg gcaactggct
     8341 ggatgtcacc gtaggcgctg atatgttgct caatcaaaat attgccgcct atgctgcgct
     8401 aactcaggca gaaaatacca ctaataatag cgactatctg tatacgatgg gggttagcgc
     8461 cagattttaa cgtaacagtc acaattgaaa ccattgaata acaacagttg tggcgatagt
     8521 gggtgctaac ttaccaaata ataaatttgg tgaataattg tcgcgtcatt cattcctgaa
     8581 ctaaggcatt tcattccgtt ctgatggcat ttcatgccgt ttttccccaa gcataaaatg
     8641 cacttcgtta tggttgtcgg cagagatttt tcctttttat tactgcagga atactgccat
     8701 gacaccttca aattatcagc gtacccgctg gctgacactc atcggtacta tcattaccca
     8761 gtttgcgctg gggtcggttt atacctggag cctgtttaat ggcgcgcttt ctgccaagct
     8821 ggatgcgccg gtaagccagg tcgctttctc tttcggcttg ttaagtctgg ggctggcaat
     8881 ttcgtcttct gttgcgggca aattgcagga acgttttggc gttaaacgcg tcaccgtggc
     8941 ttccggcatt ttgctgggat taggcttctt cctgaccgcg cattccaaca acctgatgat
     9001 gctgtggtta agcgccggtg tgctggtcgg tctggcggat ggcgcgggtt acctgctgac
     9061 gctctctaac tgcgtgaagt ggttcccgga gcgtaaaggt ctgatctccg cgttcgctat
     9121 cggttcttat ggtctgggta gcctgggttt caaatttatc gacacgcagc tgctcgaaac
     9181 ggtgggtctg gaaaaaacct ttgtgatttg gggagcgatt gcgctggtga tgattgtttt
     9241 cggcgcaacg ttaatgaaag acgcaccaaa acaggaagtg aaaaccagca atggtgtggt
     9301 ggagaaagat tacacgctgg cagagtcgat gcgtaaaccg cagtactgga tgttagcggt
     9361 aatgttcctg actgcctgca tgagcggcct gtacgtgatt ggggtagcga aagatatcgc
     9421 ccaaagtctg gcacaccttg atgtggtttc cgcagccaat gcagtaacgg ttatttccat
     9481 cgctaacctt tcaggtcgtc tggtgctggg cattctgtct gacaaaattg cccgtatccg
     9541 tgttatcacc attggtcagg tgatttcgct ggtgggtatg gcggccctgc tgtttgcacc
     9601 attaaatgca gtgacgttct ttgcagcgat tgcctgcgtg gcatttaact ttggcggcac
     9661 tattaccgtc tttccatcac tggtcagtga gttcttcggc ctcaataacc tggcgaaaaa
     9721 ctacggtgtg atttacctcg gtttcggtat cggtagcatt tttggttcga ttatcgcctc
     9781 actgtttggc ggtttctatg tgaccttcta cgtgattttc gccctgctga ttctgtcatt
     9841 ggcgctttct acgacgattc gtcagccaga gcagaaaatg ttgcgtgagg cgcatggctc
     9901 cctttaatgc tcagccgcga gctactgggc ttcagcttcc gtgtgcatca aacgcatcaa
     9961 cgaaatgtat aaacaatgac tcgtaaaatt tgggccagtt aactggccct ttttactgct
    10021 ttttgtaacg ctaaagtctc ttttcaaact tgcatttttg taaatttgtg cttcatgcac
    10081 actctttccc cacacttttt cgctttgctg tggtctactt attcgcgcgt gtagatttta
    10141 cttatctgac tacctccgca cttttcccct gccgggcctg aaaagccgct aagcagggtg
    10201 ttatcacctg tttgtccagg gtttgtttgc atgagataca tcaaatcgat tacacagcag
    10261 aagctgagct ttttgcttgc aatctatatt ggccttttta tgaatggcgc ggttttttat
    10321 cgccgcttcg gcagctatgc gcacgatttt accgtctgga aaggcgtttc tgctgttgtt
    10381 gaactggccg ccaccgtact ggtgaccttc tttttactac gtcttctatc gctgtttggc
    10441 cgccgcagct ggcgtattct ggcgtcgctg gtggtgctct tttccgcagg tgccagctat
    10501 tacatgacct tccttaatgt ggtcattggt tatggcatca tcgcttccgt catgaccacc
    10561 gatatcgacc tgtcaaaaga agttgttggt ctgaacttta ttctctggtt aatcgccgtt
    10621 agtgcattgc ctcttatcct tatctggaat aaccgctgtc gctacacctt gctccgacaa
    10681 ctgcgaaccc cagggcagcg tattcgcagc ctggtggtcg tcgtactggc gggtattatg
    10741 gtttgggcac cgattcgttt gctggatatc cagcagaaga aagtggagag ggcgaccggc
    10801 gttgatttgc cgagttatgg cggtgtcgta gcgaactctt atctgccatc aaactggctt
    10861 tctgcgttgg ggctgtatgc ctgggcgcgg gtcgatgaat cttccgataa taattcattg
    10921 cttaatccgg cgaagaagtt tacataccag gctccacaaa acgttgatga tacttatgtc
    10981 gtgtttatca tcggtgaaac cacgcgttgg gaccatatgg gtattttcgg ctatgagcgt
    11041 aataccacgc cgaaactggc ccaggagaaa aatctggcgg cgttccgtgg ttactcctgt
    11101 gataccgcaa ccaaactctc actgcgttgc atgtttgtac gtcagggggg cgcggaagat
    11161 aatccgcagc gcacattaaa agaacagaac attttcgcgg ttctgaagca gttaggattc
    11221 agttctgacc tctacgctat gcagagcgaa atgtggttct acagcaacac gatggcagac
    11281 aacattgctt atcgtgagca gattggtgcg gagccacgta atcgtggcaa gccggtagat
    11341 gatatgttgc tggtagacga aatgcagcaa tcgctagggc gcaacccgga tggtaagcat
    11401 ctgatcattc tgcataccaa aggttcgcat tttaactaca cccagcgtta tccgcgtagc
    11461 ttcgcgcagt ggaagccgga atgtattggt gttgatagcg gctgtaccaa agcgcagatg
    11521 atcaactcct atgacaactc ggtgacctat gtggatcact ttatctccag cgtgattgat
    11581 caggttcgcg ataagaaagc gattgtgttc tacgcagctg accacggtga gtcaattaat
    11641 gaacgcgagc acctgcacgg cacgccgcgt gaactggcac cgccggagca gttccgcgta
    11701 ccgatgatgg tctggatgtc agataaatat ctggaaaatc cggccaatgc gcaggcgttt
    11761 gcgcagctga aaaaagaagc cgacatgaaa gtgccacgcc gtcacgtaga gctgtacgac
    11821 accatcatgg gttgtcttgg ctatacttca ccggatggtg gaattaacga aaacaacaac
    11881 tggtgtcaca tcccgcagac aaaagtggca gcggctaact aagcggcctg ctgactttct
    11941 cgccgatcaa aaggcatttt gctattaagg gattgacgag ggcgtatctg cgcagtaaga
    12001 tgcgccccgc attcggtgat tggcgcagcc tggtagcgca cttcgttcgg gacgaagggg
    12061 tcggaggttc gaatcctcta tcaccgacca aattcgaaaa gcctgctcaa cgagcaggtt
    12121 tttttgcatt tgtagctcat gaggatgaga acctccgggg gcagggaggc tcgactcgac
    12181 gtaggcctga taagacgcgc cagcgtcgca tcaggcaacg gcaccgcact tattgtcgga
    12241 tgcggcgcga ccgccttatt cgaccaacac accacacatt ttcctgccaa ctttgcgccc
    12301 catatctcac tatccattaa cggatttgtg acagaaacca ttgtcttttt ttatatttgc
    12361 ttaaatcttt tttgcgttac ttatggctgg cgctggcatt ttccgctgtg cgttttagcg
    12421 ttcatggtat taatcgctca gatatccacc ttttcctcag atttttttca ttaaatgcat
    12481 aaaacttaat caccaaatgt tgctaaatat taagctgatt gttctggtta tgttggagta
    12541 gcacaaattt ctctgctgca aatagctgtt tgaagttttt ttaatctttg tttgtggatt
    12601 ctcaccgttg gtgtaaatcc cggttggctg tattgacgtt ttcacattct gttgacagat
    12661 tgtaggtcac gaggggcatt ttatggagga tccgcactgt tacactgatg ttaattagta
    12721 cggcatcccc acctcataac gttgacccga ccgggcaaaa aacaaaaaag gtcaggcagc
    12781 gacaacccac tgcaaagggt taaaacaaca aacatcacaa ttggagcaga ataatgcgta
    12841 tttccttgaa aaagtcaggg atgctgaagc ttggtctcag cctggtggct atgaccgtcg
    12901 cagcaagtgt tcaggctaaa actctggttt attgctcaga aggatctccg gaagggttta
    12961 acccgcagct gtttacctcc ggcaccacct atgacgcctc ttccgtcccg ctttataacc
    13021 gcctggttga atttaaaatc ggcaccaccg aagtgatccc gggcctcgct gaaaagtggg
    13081 aagtcagcga agacggtaaa acctataccc tccatctgcg taaaggtgtg aagtggcacg
    13141 acaataaaga attcaaaccg acgcgcgaaa tgaacgccga tgacgtggtg ttctcgttcg
    13201 atcgtcagaa aaacgcgcaa aacccgtacc ataaagtttc tggcggcagc tacgaatact
    13261 tcgaaggtat gggcttgccg gagctgatca gcgaagtgaa aaaggtggac gataacaccg
    13321 ttcagtttgt gctgactcga ccggaagcgc cgttcctcgc tgacctggca atggacttcg
    13381 cctctattct gtcaaaagaa tatgctgacg cgatgatgaa agccggtaca ccggaaaaac
    13441 tggacctcaa cccaatcgga accggtccgt tccagttaca gcagtaccaa aaagattccc
    13501 gtatccgcta caaagcgttt gatggctact ggggcaccaa accgcagatc gatacgctgg
    13561 tcttctctat tacccctgat gcttccgtgc gttacgcgaa attgcagaag aatgaatgcc
    13621 aggtgatgcc gtacccgaac ccggcagaca tcgctcgcat gaagcaggat aaatccatca
    13681 acctgatgga aatgccgggg ctgaacgtcg gctacctctc gtataacgtg cagaaaaaac
    13741 cgctggatga cgtgaaagtt cgccaagcac taacctacgc ggtgaacaaa gacgcgatca
    13801 tcaaagcggt ttatcagggc gcgggtgtat cagcgaaaaa cctgatcccg ccaaccatgt
    13861 ggggctataa cgacgacgtt caggattaca cctacgatcc tgaaaaagcg aaagccttgc
    13921 tgaaagaagc gggtctggaa aaaggtttct ccatcgacct gtgggcgatg ccggtacaac
    13981 gtccgtataa cccgaacgct cgccgcatgg cggagatgat tcaggcagac tgggcgaaag
    14041 tgggcgtgca ggccaaaatc gtcacctacg aatggggtga gtacctcaag cgtgcgaaag
    14101 atggcgaaca tcagacggta atgatgggct ggactggcga taacggggac ccggataact
    14161 tcttcgccac cctgttcagc tgcgccgcct ctgaacaagg ttctaactac tcaaaatggt
    14221 gctacaaacc gtttgaagat ctgattcaac cggcgcgtgc taccgacgac cataacaaac
    14281 gcgttgaact gtacaagcaa gcgcaggtgg tgatgcacga tcaggctccg gcactgatca
    14341 tcgctcactc caccgtgttt gaaccggtac gcaaagaagt caaaggctat gtggttgatc
    14401 cattaggcaa acatcacttc gaaaacgtct ctatcgaata attaaaagcc atacaagact
    14461 gatggcaaag gcaaaactgc ctgatgtgct ccgcttatca ggcctacgaa aattctgcaa
    14521 tgtattgaat ttgcacgatt ttgtaggccg gataaggcgt taacgccgca tccggcataa
    14581 acaaagcgca ctttgtcaac aatctgtata cccggtggcg ctgtgcctct gcgtgcagcg
    14641 ccatccggca gcaatacttc attctctgtc cgacaagggc gggaattgat ttgtgagcaa
    14701 tacagacacg cagttccagg ctgcgagtca ctatagagaa tccgggttat gttgcagttt
    14761 attctccgac gtttgggact cgtcatccct acgtttatcg gtattaccct tctcacattt
    14821 gcctttgtcc acatgatccc gggcgatccg gtgatgatca tggcaggcga acgtgggatc
    14881 tcccctgagc gtcacgtgca gctgctggct gaactcggct tagataaacc gatgtggcag
    14941 cagtatctcc attacatttg gggcgtaatg cacggcgatc taggcatttc aatgaaaagc
    15001 cgaattccag tatgggaaga gttcgtgccg cgctttcagg ccacgctgga acttggcgtc
    15061 tgcgcgatga tttttgctac ggcagtcggt attccggttg gtgtgctggc tgcggttaaa
    15121 cgcggttcca ttttcgatca cactgcagtt ggcctggcgc tgaccggcta ctcgatgccg
    15181 atcttctggt ggggcatgat gctgatcatg ctggtttcgg tgcactggaa cctgacgccc
    15241 gtctccggtc gcgtgagcga tatggtgttc ctcgatgact ccaatccgtt aaccggtttt
    15301 atgctaatcg acaccgccat ctggggtgaa gacggcaact ttatcgatgc cgtcgcccat
    15361 atgatcttgc ccgccattgt gctgggtact attccgctag cggtcattgt gcgtatgaca
    15421 cgctcctcga tgctggaagt gctgggcgag gattacatcc gcaccgcgcg cgccaaaggg
    15481 ctaacccgca tgcgggtgat tatcgtccat gcgctgcgta acgcgatgct gccggtagtg
    15541 accgttatcg gcctccaggt gggaacattg ctggcggggg cgattctgac cgaaaccatc
    15601 ttctcgtggc ccggtctggg gcgctggttg attgacgcac tgcaacgccg cgactatccg
    15661 gtagtgcagg gcggcgtatt gctggtggcg acgatgatta tcctcgtcaa cttgctggtc
    15721 gatctgctgt acggcgtggt gaacccgcgt attcgtcata agaagtaagg ggacatcatg
    15781 tcacaggtta ctgaaaataa agtgattagc gcaccggtgc cgatgacccc gttacaggag
    15841 ttctggcact attttaaacg caacaaaggc gcggtcgtcg ggctggttta cgtcgccatc
    15901 gtgctgttca tcgcgatctt tgccaactgg attgcgcctt ataacccggc ggatcagttc
    15961 cgcgatgcgc tgctcgcccc gccagcctgg caggaaggcg gcagtatggc acacttgctg
    16021 ggcaccgatg acgtcggtcg tgatgtgctg tcgcggctga tgtacggcgc gcgtttatcg
    16081 ctgctggttg gctgtctggt ggtggtgttg tcgctgatta tgggcgttat tctcggcctg
    16141 atcgccggtt acttcggcgg cctggttgat aacattatca tgcgcgtggt cgatatcatg
    16201 ctggcgctgc caagtctgct gttggcactg gtgctggtgg cgattttcgg cccgtcgatt
    16261 ggtaacgccg cgctggcgct gaccttcgtt gccttgccgc actatgtgcg cttaacccgc
    16321 gccgccgtgc tggtggaggt gaatcgcgat tacgtcaccg cctctcgcgt ggcaggtgcc
    16381 ggggcgatgc gccagatgtt tattaacatc ttcccgaact gccttgcgcc gctgattgtt
    16441 caggcgtcgc tcggtttctc taacgccatt ctcgatatgg ctgctcttgg tttcctcggc
    16501 atgggggcac agccgccaac gcctgagtgg ggcaccatgc tctccgacgt gttgcagttc
    16561 gcgcaaagcg cctggtgggt cgtgaccttc cctggtctgg cgatcctgtt gacggtgctg
    16621 gcatttaacc tgatgggtga cggtctgcgt gacgcgctcg atcccaaact aaagcagtaa
    16681 gaggttcgag atggcgttat taaatgtaga taaattatcg gtgcatttcg gcgacgaaag
    16741 cgcaccgttc cgcgccgtag accgcatcag ctacagcgta aaacagggcg aagtggtcgg
    16801 gattgtgggt gagtccggct ccggtaagtc ggtcagttca ctggcgatta tggggctgat
    16861 tgattatccg ggccgcgtaa tggcagaaaa actggagttt aacggccagg atttgcagcg
    16921 tatctcagaa aaagagcgcc gcaacctggt gggtgccgaa gtggcgatga tcttccagga
    16981 cccgatgacc agccttaacc cgtgctacac cgtgggtttc cagattatgg aagcgattaa
    17041 agtgcatcag ggcggcaaca aaagtacccg ccgtcagcgg gcgattgacc tgctgaatca
    17101 ggtcggtatt cccgatccgg cttcgcgtct ggatgtttac ccgcatcagc tttccggcgg
    17161 catgagccag cgcgtgatga tcgccatggc gattgcctgt cggccaaaac tgctgattgc
    17221 cgatgaaccg accaccgcgc tggacgtgac cattcaggcg caaatcatcg aactactgct
    17281 ggagctacag cagaaagaga acatggcgct ggtgttaatt acccatgacc tggcgctggt
    17341 ggcggaagct gcgcataaaa tcattgtgat gtatgcaggt caggtagtag aaacgggcga
    17401 tgcacacgcc atcttccatg cgccgcgtca cccgtatact caggcattgc tgcgtgcgct
    17461 gccagaattt gctcaggaca aagaacgtct ggcgtcgttg ccaggtgttg ttcccggcaa
    17521 gtatgaccgc ccgaacggct gcctgcttaa cccgcgctgc ccctatgcca ctgacagatg
    17581 tcgcgctgaa gaaccggcgc tgaatatgct cgctgatggg cgtcagtcca aatgccatta
    17641 cccacttgat gatgccggga ggcccacact atgagtacgc aagaggccac ctcgcaacaa
    17701 ccgctgttgc aggctatcga cctgaaaaaa cattatccgg tgaagaaagg catgttcgtg
    17761 ccggaacgtc tggtgaaggc gctggacggc gtttcgttta accttgaacg tggcaaaacg
    17821 ctggcagtag tgggcgaatc tggctgcggt aaatcgaccc tcggtcggtt gctgacgatg
    17881 atcgaaacgc ccaccggcgg cgagctgtat tatcaggggc aggatctgct caagcacgat
    17941 ccgcaggcgc agaagctgcg tcggcagaaa atccagatcg tcttccagaa tccctacggt
    18001 tcgctgaatc cgcgtaagaa agttgggcaa attctcgaag agccgctgct tattaacacc
    18061 agcttaagca aagaacagcg tcgggaaaaa gccctgtcga tgatggcaaa agtcggcctg
    18121 aaaaccgagc actatgaccg ctatccgcac atgttttccg gcggacaacg tcagcgtatc
    18181 gctattgccc gtggtctgat gctcgacccg gatgtggtga ttgccgatga gccggtttct
    18241 gcgctggacg tctcagtgcg tgcgcaggtg ctgaatctga tgatggattt gcagcaggag
    18301 ttggggcttt cttatgtctt tatctcccac gacctgtcgg ttgtggagca cattgctgat
    18361 gaagtgatgg tgatgtacct aggccgctgc gtggagaaag gaacgaagga ccaaatcttc
    18421 aataacccgc gccatccgta cacccaggcg ctgctctccg cgacgccacg cctgaacccg
    18481 gacgatcgtc gtgaacgcat caaactcacc ggtgaactgc caagcccgct gaatcctccg
    18541 ccgggttgcg ccttcaacgc ccgctgtcgt cggcgcttcg gcccctgcac ccagttgcag
    18601 ccgcagctaa aagactacgg aggtcaactg gtagcttgtt ttgctgttga tcaggatgaa
    18661 aatccgcagc gttaactcac aaaaccgggg ccatgcgctc cggtttactc aatcagcttc
    18721 aacagtggcg acaagcatag caaaatgccg taaagcagaa tcagccaggc tttaaatccg
    18781 cgaagttttt ccagttgtgc gactttatag atcaggaaaa acggaatgag gcacgacaca
    18841 atgccatata acgggcttcc caactgaaag aacaccagca ctgatacacg aaacgaaacc
    18901 caaatcgtca acgtaatgac gataaaagcg cagatcgcca gagtcagcat gcgtgagtta
    18961 attttcttgg tatcaataat tcggcttaac agattgagaa taatgccttt aatagcctcg
    19021 tggaaaccga gataaatgcc aaagaatgcg gtcagtacgg caaagatatt cagcaccgta
    19081 gaggtgatat gaatgatatg tccagggatc acctgcgcgg ccagtgccag tgccgagata
    19141 ttttgttcaa aggcagaaac ggcttcttcg tggctaattg agaaggtaaa cgaaaaggca
    19201 aaaaacagga tcaccgcgat gagcgtgata taactaattc ggtgggtacg cagcgcgagc
    19261 cgggttgcca gtactttatc tgcttcccgt ttacgatagg caatattcat tgggttaagt
    19321 acctgaataa ataccgcaga aaagaagcaa aatggaatgg taagcaggac atcgcggaaa
    19381 aagacggagg cttgcgggaa ggcggttata ttggcgaaat tccagtgcgg gatcatcgca
    19441 aaaccgaaca cgacaataat ccctactttg accaccacca ttggcccaga aatcttaaac
    19501 agtaatcgtt caccaccaga cgcaatcgct atcagtacgg cgaaaatagc gactttataa
    19561 agtagagatt gtgaaagatc ggcatcggtt aaaccgaagg ttttcaggta cgaggcgctg
    19621 tcgaaaacca cggagagaga gtagataaaa atcccgtgga taatcatcaa aaagtagata
    19681 accccgagga aaattcccca gttcttcccc aggtaatgac tgataatatc ggtgtagtca
    19741 ttacaggaat cgctttcaga aagggttttt aaataaatgt cctgcactac ccaggtgaca
    19801 ggataagcaa tgatcgctgc ggtaataaat acccaaattc ccttcaagcc aatttgtact
    19861 ggcatcagca cggttccggc accaatcgcc atgcctatgc ataataaaac ccagccgaag
    19921 tcgtagcgtg taaacggcaa tttctgattg tgtttcgata gtgtgttgtg ctgcataaag
    19981 tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa cccagtttct cttgcgcaac
    20041 aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc aacagattca ctggggaggt
    20101 cgcgaggagg cgcaattcta caaaattgcg catttcacta ccagtctgga gtcagcccgg
    20161 atatgaactg caactattgc aaataaagaa attaagctgg aaggcatcgc gcaatgagga
    20221 agattgccgg gtgctgacag taaaaagcaa tcgtagaatc agcgtcaagc cggaaggatt
    20281 ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattggttgc tggagagaga
    20341 aaacccccgc acgttgcagg tatgtacctg acaacataac gggggctaat cttgaaccta
    20401 gaccactcaa gaatagccgc gaaacgttgt cattacaaca caggcggcta tatgacgctc
    20461 gcagagctgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
    20521 gccagtatga tcgtgaactg gctgaacaag cggaagtagc gtgtcatg
//
LOCUS       DAJTRB010000079        20523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTRB010000079 DAJTRB010000000
VERSION     DAJTRB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.079"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="OO330_002962"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="OO330_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5883752.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="OO330_002963"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="OO330_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5883753.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="OO330_002964"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="OO330_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5883754.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="OO330_002965"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="OO330_002965"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5883755.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="OO330_002966"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="OO330_002966"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5883756.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="OO330_002967"
     CDS             4451..4816
                     /locus_tag="OO330_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5883757.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTNWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="OO330_002968"
     CDS             4966..5484
                     /locus_tag="OO330_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5883758.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="OO330_002969"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="OO330_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="OO330_002970"
     CDS             6715..7197
                     /locus_tag="OO330_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5883759.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="OO330_002971"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="OO330_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5883760.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="OO330_002972"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="OO330_002972"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5883761.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="OO330_002973"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="OO330_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5883762.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="OO330_002974"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="OO330_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5883763.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="OO330_002975"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="OO330_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5883764.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="OO330_002976"
     CDS             10941..11732
                     /locus_tag="OO330_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5883765.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="OO330_002977"
     CDS             11753..13039
                     /locus_tag="OO330_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5883766.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="OO330_002978"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="OO330_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5883767.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="OO330_002979"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="OO330_002979"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5883768.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="OO330_002980"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="OO330_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5883769.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="OO330_002981"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="OO330_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5883770.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="OO330_002982"
     CDS             complement(16987..17277)
                     /locus_tag="OO330_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5883771.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="OO330_002983"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="OO330_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5883772.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="OO330_002984"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="OO330_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5883773.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="OO330_002985"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="OO330_002985"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="OO330_002986"
     CDS             19139..20380
                     /locus_tag="OO330_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5883774.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5301 a         5363 c         4813 g         5046 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctag aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatctgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accaactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttacc tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 tatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattacaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aggaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtc ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtgtt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcccccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DAJTRB010000080        20288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTRB010000080 DAJTRB010000000
VERSION     DAJTRB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.080"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="OO330_002987"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="OO330_002987"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5883775.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="OO330_002988"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="OO330_002988"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5883776.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3000)
                     /locus_tag="OO330_002989"
     CDS             complement(1729..3000)
                     /locus_tag="OO330_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5883777.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3183..4388)
                     /locus_tag="OO330_002990"
     CDS             complement(3183..4388)
                     /locus_tag="OO330_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5883778.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAEHFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="OO330_002991"
     CDS             complement(4488..4844)
                     /locus_tag="OO330_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5883779.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="OO330_002992"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO330_002992"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="OO330_002993"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="OO330_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5883780.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="OO330_002994"
     CDS             6774..9830
                     /locus_tag="OO330_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5883781.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="OO330_002995"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="OO330_002995"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5883782.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="OO330_002996"
     CDS             10337..10525
                     /locus_tag="OO330_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5883783.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="OO330_002997"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="OO330_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5883784.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="OO330_002998"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="OO330_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5883785.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="OO330_002999"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="OO330_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5883786.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="OO330_003000"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="OO330_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5883787.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="OO330_003001"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="OO330_003001"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5883788.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="OO330_003002"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="OO330_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5883789.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="OO330_003003"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="OO330_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5883790.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO330_003004"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO330_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5883791.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="OO330_003005"
     CDS             complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="OO330_003005"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5883792.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5315 a         4860 c         5177 g         4936 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaatg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat gtgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgactgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaatt ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgttcgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgacctg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    18301 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    18361 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg
    18421 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    18481 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    18541 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    18601 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
    18661 tcagcagttt caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca
    18721 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
    18781 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
    18841 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    18901 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
    18961 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    19021 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    19081 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    19141 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    19201 tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    19261 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    19321 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    19381 tcgtcaacca gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc
    19441 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    19501 gctttcagag aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    19561 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    19621 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    19681 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    19741 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    19801 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
    19861 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
    19921 acgtgagtgt ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
    19981 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
    20041 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
    20101 aaaggttacg caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga
    20161 ggataagtga aagttcatcc ggtgtataaa tatatcatcg ctcaattgct acagttaaaa
    20221 taatttatcg cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattgata
    20281 tatgctga
//
LOCUS       DAJTRB010000081        19691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTRB010000081 DAJTRB010000000
VERSION     DAJTRB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.081"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO330_003006"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO330_003006"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5883793.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO330_003007"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO330_003007"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5883794.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO330_003008"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO330_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5883795.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO330_003009"
     CDS             6038..6658
                     /locus_tag="OO330_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5883796.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO330_003010"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO330_003010"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5883797.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO330_003011"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO330_003011"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5883798.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO330_003012"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO330_003012"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5883799.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGACFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO330_003013"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO330_003013"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5883800.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO330_003014"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO330_003014"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO330_003015"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO330_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO330_003016"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO330_003016"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5883801.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO330_003017"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO330_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5883802.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO330_003018"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO330_003018"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5883803.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO330_003019"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO330_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5883804.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO330_003020"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO330_003020"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5883805.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO330_003021"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO330_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5883806.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4681 a         5503 c         5033 g         4474 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc acgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcagcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ccttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctaccagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cacgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggtgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc ggcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcatcgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg c
//
LOCUS       DAJTRB010000082        19167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTRB010000082 DAJTRB010000000
VERSION     DAJTRB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.082"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            111..2056
                     /locus_tag="OO330_003022"
                     /pseudo
     CDS             111..2056
                     /locus_tag="OO330_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            2206..2637
                     /gene="ndk"
                     /locus_tag="OO330_003023"
     CDS             2206..2637
                     /gene="ndk"
                     /locus_tag="OO330_003023"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5883807.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2787..3941
                     /gene="trmG/rlmN"
                     /locus_tag="OO330_003024"
     CDS             2787..3941
                     /gene="trmG/rlmN"
                     /locus_tag="OO330_003024"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5883808.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTCQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4226..5239
                     /gene="rodZ"
                     /locus_tag="OO330_003025"
     CDS             4226..5239
                     /gene="rodZ"
                     /locus_tag="OO330_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5883809.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5266..6393
                     /gene="ispG"
                     /locus_tag="OO330_003026"
                     /gene_synonym="gcpE"
     CDS             5266..6393
                     /gene="ispG"
                     /locus_tag="OO330_003026"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5883810.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6496..7770
                     /gene="hisS"
                     /locus_tag="OO330_003027"
     CDS             6496..7770
                     /gene="hisS"
                     /locus_tag="OO330_003027"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5883811.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7788..8408
                     /gene="yfgM"
                     /locus_tag="OO330_003028"
     CDS             7788..8408
                     /gene="yfgM"
                     /locus_tag="OO330_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5883812.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8419..9597
                     /gene="bamB"
                     /locus_tag="OO330_003029"
     CDS             8419..9597
                     /gene="bamB"
                     /locus_tag="OO330_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5883813.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9715..11187
                     /gene="der"
                     /locus_tag="OO330_003030"
     CDS             9715..11187
                     /gene="der"
                     /locus_tag="OO330_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5883814.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11257..11472
                     /locus_tag="OO330_003031"
     CDS             11257..11472
                     /locus_tag="OO330_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5883815.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11469..12839)
                     /gene="xseA"
                     /locus_tag="OO330_003032"
     CDS             complement(11469..12839)
                     /gene="xseA"
                     /locus_tag="OO330_003032"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5883816.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13001..14467
                     /gene="guaB"
                     /locus_tag="OO330_003033"
     CDS             13001..14467
                     /gene="guaB"
                     /locus_tag="OO330_003033"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5883817.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14536..16113
                     /gene="guaA"
                     /locus_tag="OO330_003034"
     CDS             14536..16113
                     /gene="guaA"
                     /locus_tag="OO330_003034"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5883818.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16208..16747)
                     /locus_tag="OO330_003035"
     CDS             complement(16208..16747)
                     /locus_tag="OO330_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5883819.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16763..17281)
                     /locus_tag="OO330_003036"
     CDS             complement(16763..17281)
                     /locus_tag="OO330_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5883820.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17592..17783)
                     /locus_tag="OO330_003037"
     CDS             complement(17592..17783)
                     /locus_tag="OO330_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5883821.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17801..17953
                     /locus_tag="OO330_003038"
     CDS             17801..17953
                     /locus_tag="OO330_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883822.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18135..>19167
                     /gene="pdeF"
                     /locus_tag="OO330_003039"
     CDS             18135..>19167
                     /gene="pdeF"
                     /locus_tag="OO330_003039"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5883823.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRAL"
BASE COUNT         4522 a         4666 c         5209 g         4770 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg caaaactgga
     1081 agatatggcg gcagcgtata ccgcgtttgc tcgccacggc aaggcaggca aattgcgctt
     1141 acagcctgac gatccgctgc ttgaacggcc tttaatgtcg tcgggggcgg cgtggatcat
     1201 tcgccggatt atggctgatg aagcgcaacc cttgccggat agtgccttgc cgcgcgtcgc
     1261 cccactggca tggaaaacgg gcaccagcta tggctatcgt gacgcctggg cgattggggt
     1321 taacgctcgc tatgtcattg ggatctggac tggcagacct gacggcacgc ccgttgttgg
     1381 tcagtttggc tttgccagtg ccgtaccatt gttgaatcag gtcaataata tcttactgtc
     1441 gcgcagtgcg aatctgccgg aagacccgcg cccgaactca gtcactcgtg gcgttatctg
     1501 ctggccgggc ggacagtctt tgccggaagg tgacggtaac tgtcgccgcc gcctggcaac
     1561 ctggctgctg gatggcagcc agccgccaac gctgttactg tcagagcagg aagggattaa
     1621 tggcattcgc ttccccatct ggctggatga caaagggcag cgcgtcgccg ctgactgccc
     1681 gcaggcgaag caagaaatgg taaatgtctg gccgctaccg ctggaaccct ggctgcccgc
     1741 atcagaacgt cgcgctgtgc gcttaccacc ggcctcgaca atttgcccgc catacggtca
     1801 cgatgcccag ctccctctgc agctgacagg cgttcgcgat ggcgcgatta ttaaacgttt
     1861 accgggcgca gcggaagcga ctttgccgtt gcaatcaagt ggaggggcag gtgaacgctg
     1921 gtggtttctg aatggcgaac cgttaactga acgcgggcgc aacgtgaccc tgcatttgac
     1981 ggataaaggt gattatcaat tgctggtgat ggatgatgtg gggcaaatcg cgacagtgaa
     2041 atttgtcatg caatagtctg ttttgtccct gattgttgct aaaactcatt ttattttaaa
     2101 aaaaatgtta ccttcatcaa tagtcaacgg ccctgttgct cattataatc cgcgccatct
     2161 cgtacgctgg tacagacaac aacagaacaa tttacagagg taaaaatggc tattgaacgt
     2221 actttttcca tcatcaaacc gaacgcggta gcaaaaaacg tcattggtaa tatctttgcg
     2281 cgctttgaag ctgcagggtt caaaattgtc ggcaccaaaa tgctgcatct gaccgttgaa
     2341 caggcacgtg gcttttatgc tgaacacgat ggaaaaccgt tctttgatgg tctggttgaa
     2401 ttcatgacct ctggcccgat cgtggtttcc gtgctggaag gtgaaaacgc cgttcagcgt
     2461 caccgcgatc tgctgggcgc gaccaatccg gcaaacgcac tggctggtac tctgcgcgct
     2521 gattacgctg acagcctgac cgaaaacggt acccacggtt ctgattccgt cgaatctgcc
     2581 gctcgcgaaa tcgcttattt cttcggcgaa ggcgaagtgt gcccgcgcac ctgttaataa
     2641 tttcgtaaat gccgcgtgca aacgtggcat ccgtgcgcca gaatttgtac aatgcagcgc
     2701 ccccggacga gcagccgctc accggggcgt ttcttttttc aaccctccag gggccataac
     2761 gtgtaataac gaggccggaa tagcatatgt ctgaacaatt agtcacacct gaaaacgtca
     2821 ccacgaaaga tggaaaaatc aacctgctgg atctcaaccg tcagcagatg cgggagtttt
     2881 ttaaagattt aggtgaaaaa accttccgcg ccgatcaggt gatgaagtgg atgtatcact
     2941 attgctgcga caactttgat gagatgaccg acatcaacaa agtgttgcgc ggcaaactga
     3001 aagaggtggc ggaaatccgc gcaccggaag tggttgaaga acagcgttca tccgacggca
     3061 ccattaaatg ggcgatcgcc gttggcgatc agcgcgtcga aacggtgtat atcccggaag
     3121 acgaccgtgc cacgctctgc gtctcttcgc aggtggggtg tgcgctggag tgtaaattct
     3181 gttccaccgc ccagcagggc tttaaccgca acctgcgggt atcggaaatt attggccagg
     3241 tgtggcgtgc ggcgaaaatc gtcggcgcag caaaagtcac ctgtcagcgc ccaatcacta
     3301 acgtcgtgat gatgggcatg ggcgagccgc tgctcaacct gaacaacgtc gttccggcga
     3361 tggaaatcat gcttgatgat ttcggttttg gcctgtctaa acgtcgcgtg acgctttcca
     3421 cttccggtgt agttccggcg ctggataaac tgggcgatat gatcgacgtt gcactggcaa
     3481 tttccctgca cgcgccgaac gacgaaattc gtgacgaaat tgtgccgatc aacaaaaagt
     3541 acaatatcga aacgttcctt gctgcggtgc gccgttatct ggagaaatcc aacgccaatc
     3601 agggccgagt cactattgaa tacgtgatgc ttgaccacgt taacgacggc actgaacacg
     3661 cgcaccaact ggcggaactg ctgaaagata cgccgtgtaa gatcaacctg atcccgtgga
     3721 acccgttccc ggacgcgccg tatggacgca gttcgaacag ccgtatcgac cgtttctcaa
     3781 aagtgctgat gagctacggt ttcaccacta ttgtgcgtaa aacgcgtggt gatgatatcg
     3841 atgctgcctg tggtcagttg gcgggcgatg ttatcgaccg tacgaaacgt actctgcgta
     3901 aacggatgca gggtgaagcc atcgatatta aagcggtctg atagccatta cgccacggtt
     3961 actttatgta ccgtggcgta atcttttatg aatgaagtat taagttgcaa ttgtctggtc
     4021 aatattaacg gtgcgttttt gctgacttta aggcagtatg taacgatgca acagtaagtt
     4081 agcctcagtg aatgtgggct ttgtcacgag cacacagacg gtcttatact gtatgataac
     4141 ggttaactta acggatgttt cgcggtgtgg gtgagcatga tggttcaccg gcatctcaat
     4201 tctcatttaa acgtacctgc agcgaatgaa tactgaagcc acgcacgacc aaaatgaagc
     4261 acttactacc ggcgctcgcc tgcgtaatgc tcgcgaacaa ctaggactta gtcagcaggc
     4321 cgttgccgag cgactttgcc tgaaggtttc cacggtacgc gacattgaag aagataaggc
     4381 acccgccgat cttgcttcaa cattcctgcg cggatatatc cgctcttatg cgcgtctggt
     4441 acatattcca gaagaagaac tgctgccagg gctggaaaag caggctccac ttcgggctgc
     4501 aaaagttgcg ccaatgcaga gtttttccct cggtaaacgc cgcaaaaaac gcgacggctg
     4561 gctaatgacc ttcacctggc tggtgttgtt tgtggttatc ggcctgagcg gtgcctggtg
     4621 gtggcaagac cacaaagctc agcaggaaga gatcaccact atggccgatc aatcttcggc
     4681 ggaactgagc agtaatagcg agcaggggca gagtgttccg ttaaatacgt cgacaactac
     4741 agacccggct acaacgtcca cgccgccagc gtctgtggat actaccgcaa ccaacacgca
     4801 aacacctgcc gtaactgcgc cagcaccagc tgttgatccg caacagaatg cggttgtttc
     4861 gccttcgcag gcaaatgttg ataccgccgc gaccccggta ccaacggcag caacaacgcc
     4921 agatggtgct gcgccgttgc caaccgatca ggctggcgtg accacgccgg cggctgatcc
     4981 gaatgcgctg gtgatgaact ttactgccga ttgctggctg gaggtcactg atgctaccgg
     5041 taaaaaattg tttagcggga tgcagcgtaa agacggtaat ttgaatttaa ccggccaggc
     5101 accgtataaa ctgaaaattg gtgcgccagc cgcagtacag atccagtatc aagggaaacc
     5161 tgtcgatctg agtcgtttta tcagaactaa ccaggttgcg cgtctgaccc tcaatgccga
     5221 acaatcaccg gcgcagtaac agacgggtaa cgcgggagat ttttcatgca taaccaggct
     5281 ccaattcaac gtagaaaatc aacacgtatt tacgttggga atgtgccgat tggcgatggt
     5341 gctcccatcg ccgtacagtc catgaccaat acgcgtacga cagatgtcga agcaacggtc
     5401 aatcaaatca aggcgctgga acgcgttggc gctgatatcg tccgtgtctc cgtaccgacg
     5461 atggacgcgg cagaagcgtt caaactcatc aaacagcggg ttaacgtgcc gctggtggct
     5521 gacatccact tcgactatcg cattgcgctg aaagtagcgg aatacggcgt cgattgtctg
     5581 cgtattaacc ctggtaatat cggtaatgaa gagcgtattc gcatggtggt tgactgtgcg
     5641 cgcgataaaa acattccgat ccgtattggc gttaacgccg gatcgctgga aaaagatctg
     5701 caagaaaagt atggcgaacc gacgccgcag gcgttgctgg aatccgccat gcgtcatgtt
     5761 gatcatctcg atcgcctgaa cttcgatcag ttcaaagtca gcgtgaaagc gtctgacgtc
     5821 ttcctcgctg ttgagtctta tcgtttgctg gcaaaacaga tcgatcagcc gctgcatctg
     5881 gggatcaccg aagccggtgg tgcacgcagc ggggcagtaa aatccgccat tggtttaggt
     5941 ctgctgctgt cggaaggcat cggcgacacg ctgcgtgtat cgctggcggc cgatccggtc
     6001 gaagagatca aagtcggttt cgatattttg aaatcgctgc gtatccgttc gcgagggatt
     6061 aacttcatcg cctgcccgac ctgttcgcgt caggaatttg atgttatcgg tacggttaac
     6121 gcgctggagc aacgcctgga agatatcatc actccgatgg acgtttcgat tatcggctgc
     6181 gtggtgaatg gcccaggtga ggcgctggtt tctacactcg gcgtcaccgg cggcaacaag
     6241 aaaagcggcc tctatgaaga tggcgtgcgc aaagaccgtc tggacaacaa cgatatgatc
     6301 gaccagctgg aagcacgcat tcgtgcgaaa gccagtcagc tggacgaagc gcgtcgaatt
     6361 gacgttcagc aggttggaaa aataataacg tgatgggaag cgcctcgctt cccgtgtatg
     6421 attgaacccg catggctccc gaaacattga gggaagcgtt gagggttcat ttttatattc
     6481 agaaagagaa taaacgtggc aaaaaacatt caagccattc gcggcatgaa cgattacctg
     6541 cctggcgaaa cggccatctg gcagcgcatt gaaggcacac tgaaaaacgt gctcggcagc
     6601 tacggttaca gtgaaatccg cttgccgatt gtagagcaga ccccgctatt caaacgtgcg
     6661 attggtgaag tcaccgacgt ggttgaaaaa gagatgtaca cctttgagga tcgcaatggc
     6721 gacagcctga ctctgcgccc tgaagggacg gcgggctgtg tacgcgccgg catcgagcat
     6781 ggtcttctgt acaatcagga acagcgtctg tggtatatcg ggccgatgtt ccgtcacgag
     6841 cgtccgcaga aagggcgtta tcgtcagttc catcagttgg gctgcgaagt tttcggtctg
     6901 caaggtccgg atatcgacgc tgaactgatt atgctcactg cccgctggtg gcgtgcgctg
     6961 ggtatcttcg aacacgtaac tcttgagctg aattctatcg gttcgctgga agcacgcgcc
     7021 aattaccgcg atgcgctggt ggcattcctt gagcagcata aagaaaagct ggacgaagac
     7081 tgcaaacgcc gcatgtacac taacccgctg cgcgtgctgg attcaaaaaa tccggaagtg
     7141 caggcgcttc tcaacgacgc tccggcatta ggtgattatc tggacgagga atctcgtgag
     7201 cattttgccg gtctgtgcaa actgctggag agcgcgggga tcgcttacac cgtaaaccag
     7261 cgtctggtgc gtggtctgga ttactataac cgtaccgttt tcgagtgggt gactaacagt
     7321 ctcggctccc agggcaccgt gtgtgcaggc ggtcgttatg acggtcttgt ggaacaactg
     7381 ggcggtcgtg caacaccggc tgtcggtttt gcgatgggcc tcgaacgtct tgtattgtta
     7441 gtacaggccg ttaatccgga atttaaagcc gatcctgttg tcgatatata cctggtggct
     7501 tcaggtgctg atacacaatc tgcggctatg gcattagctg agcgtctgcg tgatgaatta
     7561 ctgggcgtga aattgatgac caaccacggc ggcggcaact ttaagaaaca gtttgcccgt
     7621 gctgataaat ggggtgcccg cgttgctgtg gtgctgggtg agtctgaagt ggctaacggc
     7681 acagcagtag tgaaggattt gcgctctggt gagcaaacgg cagttgcgca ggatagcgta
     7741 gccgcgcatt tgcgcacgtt actgggttaa ggaaggagaa ggacagcgtg gaaatttacg
     7801 agaacgaaaa cgaccaggta gaagcggtta aacgcttttt tgctgaaaat ggcaaagcac
     7861 tggctgttgg ggggattttg ggcgttggcg cactgattgg ctggcgctac tggaacagcc
     7921 atcaggttga ttctgcacgc tccgcttctc ttgcctatca aaatgcggtt accgcagtga
     7981 gcgaaggcaa accggatagc atcccggcgg cggaaaaatt tgctgctgaa aataaaaata
     8041 cttatggtgc gctggcttct ttggaacttg cgcagcaatt tgttgacaaa aatgaactag
     8101 agaaagctgc cgcccagtta caacaggggc tggcagacac gagcgatgaa aatctcaaag
     8161 ccgtgataaa tctgcgtctt gctcgcgttc aggtacagct caagcaggct gatgccgcgc
     8221 tgaaaaccct tgataccatc aaaggtgaag ggtgggctgc cattgttgcc gacttgcgtg
     8281 gtgaagcatt gctgagcaaa ggtgataagc aaggtgcgcg tagtgcatgg gaagcaggcg
     8341 tgaaaagcga tgttactccg gcactgagcg aaatgatgca gatgaaaatt aataatttgt
     8401 ccatctgaga gggacccgat gcaattgcgt aaattactgc tgccaggact gctttccgtt
     8461 acccttttaa gcggctgttc gctgtttaac agcgaagaag atgtggtaaa gatgtcccca
     8521 ttgccaaccg ttgaaaacca gtttacgccg accacggcgt ggagcacttc cgttggtagc
     8581 ggcattggca acttctattc caatcttcat ccggcactgg cggacaacgt tgtctatgca
     8641 gcggaccgcg ctggtttggt aaaagcgctg aatgcggatg atggcaaaga aatctggtct
     8701 gtcagcctgg ccgagaaaga tggctggttc tctaaagagc ctgcattact ttctggcggt
     8761 gtgaccgtgt ctggtgggca tgtctacatt ggcagcgaaa aggcgcaggt ttacgcgctg
     8821 aataccagcg atggtactgt ggcatggcaa actaaagtcg cgggtgaagc actttcgcgc
     8881 ccggtggtca gcgacggtct ggtgttaatc cacaccagta acggtcagtt acaagcgctg
     8941 aacgaagctg acggcgctgt caaatggaca gttaacctcg atatgccttc gctctctttg
     9001 cgtggcgagt ctgcgccggc aacggctttt ggtgcggccg tcgtgggtgg cgataatggt
     9061 cgcgtcagcg cagtgctgat ggaacagggc cagatgattt ggcagcagcg tatttcccag
     9121 gcgaccggtt ctaccgaaat tgaccgtctg agcgatgttg acacgactcc cgtcgttgtt
     9181 aacggcgttg ttttcgcgct ggcctataat ggtaacctga cggcgcttga tctgcgcagt
     9241 ggtcagatta tgtggaaacg cgaactgggt tcggtgaatg atttcatcgt cgatggcaat
     9301 cgcatctatc tggtcgatca aaatgaccgg gtgatggcgt tgaccattga tggcggcgtt
     9361 acgctgtggg cacaaagcga tctgctgcat cgcctgctga cttctccggt gctgtataat
     9421 ggcaacctgg tggtcggtga cagtgaaggt tatctgcact ggattaacgt cgaagatggt
     9481 cgtttcgttg cccagcaaaa agttgatagt tccggtttcc agactgaacc ggttgccgct
     9541 gacggcaaac tgctgatcca ggcaaaagac ggaaccgtgt actctattac acgttaatcg
     9601 tctctgtcgt tcactttgaa aacggctcct ggacaggggc cgttttcctg tttttaacaa
     9661 cgacgcgaat atagcgtgcg ttgtctgatg atttataaaa atgaggcttt aaacatggta
     9721 cctgtggtcg cgcttgtcgg gcgccctaac gtaggaaaat ccacgttatt taaccgtcta
     9781 actcgcaccc gagatgcgct ggttgcggat ttcccgggtc tgactcgtga ccgtaagtac
     9841 ggtcgtgcgg aaattgaagg ccgtgagttt atctgtattg ataccggcgg gattgatggc
     9901 acagaagacg gtgtagaaac ccgcatggcg gaacagtcgc tgctggcgat tgaagaagcg
     9961 gacgtcgtac tgtttatggt ggatgcgcgc gcgggcctga tgccggcaga tgaagcgatt
    10021 gccaaacatc tgcgctcccg tgaaaaaccg accttcctgg tggtaaacaa aactgacggt
    10081 ctggatcccg atcaggcagt ggttgatttc tactcgcttg gtttaggtga aatctacccg
    10141 atcgccgcgt ctcacggtcg tggcgtatta agtctgctgg agcatgtgct gctgccgtgg
    10201 atggaagatc tcgcaccgca agaggaagtc gacgaagacg ctgaatactg ggcgcaattt
    10261 gaagcggaag agaacggcga agaagaagag gaagacgact tcgacccgca aagtctgccg
    10321 atcaaactgg cgattgtggg tcgtccgaac gtaggtaagt ctacactcac taaccgtatt
    10381 cttggagaag agcgcgttgt tgtttacgac atgcctggca cgacgcgtga cagcatctac
    10441 atcccaatgg aacgcgatgg acgtgagtat gtgctcattg acaccgctgg cgtacgtaaa
    10501 cgcggcaaaa tcaccgatgc tgtagagaaa ttctccgtaa tcaaaacgtt gcaggccatt
    10561 gaagacgcca acgtggtgat gttagtgatt gatgcgcgcg aaggtatttc cgatcaggat
    10621 ctctcgctgc tgggctttat tctcaatagt gggcgctcac ttgtcattgt ggtgaataag
    10681 tgggatggcc tgagccagga agtgaaagag caggtgaaag aaacgctgga cttccgtctg
    10741 ggctttatcg attttgctcg tgtgcacttt atctctgcct tgcacggcag tggtgttggt
    10801 aacttgtttg aatcagtacg tgaagcgtat gacagctcca cccgtcgtgt ggggacctct
    10861 atgctgacgc gcatcatgac gatggctgtt gaagatcacc aaccgccgct ggtacgcggt
    10921 cgtcgtgtga agctgaaata tgcccacgct ggtggttata acccgccgat tgtggtgatt
    10981 cacggtaatc aggtgaaaga cctgcctgat tcctacaagc gctacttgat gaactacttc
    11041 cgcaaatcgc tggacgtaat gggatcgccg attcgtattc agttcaaaga aggggaaaac
    11101 ccgtatgcga acaagcgtaa caccctgacg ccaacccaga tgcgtaaacg taagcgtctg
    11161 atgaagcaca tcaagaaaaa taaataatta tccgtaagta ttttatctgc cattccctct
    11221 atgaatgtag agggaatggc taccggagga attcatatgg aactgcattg tccaaaatgt
    11281 cagcatgtgc ttgatcagga taacggccat gcgcgttgcc catcctgcgg ggaatttatt
    11341 gaaatgaaag ctctttgtcc agactgccat caaccgttgc aggtgttaaa agcctgcggt
    11401 gcagtagatt acttctgtca gcacgggcat ggcttgatat cgaaaaaacg cgttgaattc
    11461 gtgctggctt aatgaacctt tttacgcgat ttttttaccg gctggatgtt ttttacttca
    11521 ctttctatcc agccgtcttc cagacgtgtg gttagcattt cacccgtttt aacttgcttc
    11581 acttttttca gtacattgcc gtcagtagca gtagtaacgc tgtatccacg cgccagcgtt
    11641 gacagtgggc ttacggcttc gaggtgcgtt actgcattac cgaaacgttc acgcgtggcg
    11701 ctaagctgtg cgcgcaggat ttctgctaaa cgatattcca gttgctgaat gcgcgtttgc
    11761 gtgcgatgaa tcttcggctg tggattttgc tgattcagcc gctgtgttaa tcgctgctgc
    11821 tgttgcccgg cacgcttaag ctgattttcc agcgcaaagc tcatccgttt ttgcaggcgt
    11881 tcaagcatgg tttgctggcg tgccagccgg agctgcggat gctgctgctg caagcgatga
    11941 tgaatctgcg taaagcgacg tgtacggttg gcgagataat aatccatcgc catctccagc
    12001 cgttgacggg ccgattgcac ctggcgcagt aactcttgct gattacggct cactacttca
    12061 gcggcggcag acggcgttgg cgcacgcaga tcggcaacaa aatcggcaat ggtcacatcc
    12121 gtctcatgcc cgacggcgct gacgaccgga atgcggctgg caaaaatcgc ccgcgctacg
    12181 cgttcgtcgt taaaactcca taaatcttcc agcgaaccgc cgccgcgccc aacgatcaac
    12241 acgtcgcact cattgcgctg attcgccagt tcaatggcgc gaacaatttg ccccggcgcg
    12301 tcatcgccct gaacggaggt ggggtagatg atcaccggta gagaaggatc gcgacgtttt
    12361 aacacatgca aaatatcatg tagcgcagca ccggtttttg aggtgatcac accaacgcaa
    12421 tgcgcagggg agggaagtgg ttttttgtat tgctgatcga acaaaccttc agcctgcaac
    12481 ttcgctttga gctgttcgta cttctgttgc agcagccctt caccggccgg ctgcatactc
    12541 tcaacgatta tctggtagtc gccgcgcggc tcgtagagcg taatattggc gcgaactaaa
    12601 acttgttgcc catgctgcgg acggaaggtg acccggcggt tgctgttgcg gaacatcgcg
    12661 cagcgtacct gggcggtgtc gtctttgagt gtaaagtacc agtgaccgga agccggttgt
    12721 gtgaaattag agatttcgcc gctgatccaa acctgtccca tctcatgctc aagcagcaga
    12781 cgaaccgttt gattcaggcg actaacggta aaaattgcag gggattgaga aggtaacatg
    12841 tgagcgagat caaattctaa atcagcaggt tattcagtcg atagtaaccc gcccttcggg
    12901 gatagcaagc attttttgca aaaaggggta gatgcaatcg gttacgctct gtataatgcc
    12961 gcggcaatat ttattaacca ctctggtcga gatattgccc atgctacgta tcgctaaaga
    13021 agctctgacg tttgacgacg ttctcctcgt tcctgctcat tctaccgttc tgccgaatac
    13081 tgctgacctc agcacccagc tgacgaaaac tattcgtctg aatatcccta tgctttccgc
    13141 agcaatggat accgtaacgg aagcgcgcct ggctattgct ctggctcagg aaggcggtat
    13201 cggctttatc cacaaaaaca tgtccattga acgccaggca gaagaagttc gccgtgtgaa
    13261 aaaacacgaa tctggtgtgg tgactgatcc gcagactgtt ctgccaacca cgacgctgcg
    13321 cgaagtgaaa gaactgaccg agcgtaacgg ttttgcgggc tatccggtcg ttaccgaaga
    13381 aaacgaactg gtgggtatta tcaccggtcg tgacgtgcgt tttgttaccg acctgaacca
    13441 gccggttagc gtttacatga cgccgaaaga gcgtctggtc accgtgcgtg aaggtgaagc
    13501 ccgtgaagtg gtgctggcaa aaatgcacga aaaacgcgtt gaaaaagcgc tggtggttga
    13561 tgacgaattc cacctgatcg gcatgatcac cgtgaaagac ttccagaaag cggaacgtaa
    13621 accgaacgcc tgtaaagacg agcaaggccg tctgcgtgtt ggtgctgcag ttggcgcagg
    13681 tgcgggtaac gaagagcgtg ttgacgcgct ggttgccgct ggcgttgacg ttctgctgat
    13741 cgactcctcc cacggtcact cagaaggcgt tttgcagcgt atccgtgaaa cccgtgctaa
    13801 atatccggat ctgcaaatca tcggcggcaa cgtggcaaca gctgcaggtg cacgcgcttt
    13861 ggcagaagct ggttgcagcg cggttaaagt cggtatcggc cctggttcta tctgtacgac
    13921 tcgtatcgtt actggcgtag gtgttccgca gatcactgcc gttgctgacg cagttgaagc
    13981 cctggaaggc accggtattc cggttatcgc agatggtggt attcgcttct ccggcgacat
    14041 cgccaaagct atcgccgctg gcgcaagcgc ggtgatggta ggttccatgc tggcgggtac
    14101 tgaagaatct ccgggtgaaa tcgaactcta ccagggccgt tcttataaat cttaccgtgg
    14161 tatgggttcc ctgggcgcga tgtccaaagg ttcctctgac cgttatttcc agagcgataa
    14221 cgctgccgac aaactggtgc cggaaggtat cgaaggtcgc gtagcttata aaggtcgcct
    14281 gaaagagatc attcaccagc agatgggcgg cctgcgctcc tgtatgggtc tgaccggctg
    14341 tggtactatc gacgaactgc gtactaaagc ggagtttgta cgtatcagcg gtgcgggcat
    14401 tcaggaaagc cacgttcacg acgtgaccat tactaaagag tccccgaact accgtctggg
    14461 ctcctgattc tcttcgcccg atttcatgtc gggcgattta tattatctgt ttcacttgcc
    14521 tcggaataag cgtcaatgac ggaaaacatt cataagcatc gcatcctcat tctggacttc
    14581 ggttctcagt acactcaact ggttgcgcgc cgcgtgcgtg agctgggtgt ttactgcgaa
    14641 ctgtgggcgt gggatgtgac agaagcacaa attcgtgact tcaatccaag cggaattatt
    14701 ctttccggcg gcccggaaag tactactgaa gaaaacagtc cgcgtgcgcc gcagtatgtc
    14761 tttgaagcag gcgtaccgat attcggcgtt tgctatggca tgcagaccat ggcaatgcag
    14821 ttgggcggtc acgttgaagc ctctaacgaa cgtgaatttg gctacgcgca ggtagaagtc
    14881 gtaaacgaca gcgcactggt tcgcggtatc gaagatgcgc tgaccgcaga cggtaaaccg
    14941 ctgctcgatg tctggatgag ccacggcgat aaagttaccg ctatcccgtc cgacttcgtc
    15001 accgtagcca gcaccgaaag ctgcccgttt gccattatgg ctaacgaaga aaaacgcttc
    15061 tatggcgtac agttccaccc ggaagtgact cacacccgcc agggtatgcg catgctggag
    15121 cgttttgtgc gtgatatctg ccagtgtgaa gccctgtgga cgccagcgaa aattatcgac
    15181 gatgctgtag ctcgcatccg cgagcaggta ggcgacgata aagtcatcct cggcctctct
    15241 ggtggtgtgg attcctccgt aaccgcaatg ctgctgcacc gtgctatcgg taaaaacctg
    15301 acttgcgtat tcgtcgacaa cggcctgctg cgtctcaacg aagcagagca ggttctggat
    15361 atgtttggcg atcactttgg tcttaacatt gttcacgtac cggcagaaga tcgcttcctg
    15421 tcagcgctgg ctggcgaaaa cgatccggaa gcaaaacgta aaatcatcgg gcgcgttttc
    15481 gttgaagtat tcgatgaaga agcgctgaaa ctggaagacg tgaagtggct ggcgcagggc
    15541 accatctacc ctgacgttat cgaatctgcg gcttctgcaa ccggtaaagc acacgtcatc
    15601 aaatctcacc acaacgtggg cggcctgccg aaagagatga agatgggcct ggttgaaccg
    15661 ctgaaagagc tgttcaaaga cgaagtgcgt aagattggtc tggagctggg cctgccgtac
    15721 gacatgctgt accgtcatcc gttcccggga ccaggccttg gcgttcgtgt tctgggtgaa
    15781 gtgaagaaag agtactgtga cctgctgcgc cgtgctgacg ccatcttcat tgaagaactg
    15841 cgtaaagcgg acctgtacga caaagtcagc caggcgttca ccgtcttcct gccagtacgt
    15901 tccgttggcg taatgggcga tggtcgtaag tatgactggg ttgtctctct gcgtgctgtc
    15961 gaaaccatcg actttatgac cgcacactgg gcgcatctgc cgtacgattt cctcggtcgc
    16021 gtttccaacc gcattatcaa tgaagtgaac ggtatttccc gcgtggtgta tgacatcagc
    16081 ggcaagccgc cagctaccat tgagtgggaa tgattttgat ccctgcacta tgaatgaaca
    16141 aaaccctccg ttactacaga gggtttttta tcttcaagaa tcataggctt gaagttacta
    16201 acatcgatta attaaaccag ctgtccgatt tgttctcttc tgctttgccc acgcttttca
    16261 tcagatcgcg accgccttca gtcatatttc tgtttgcgtc agcttcagat tgcaccacat
    16321 cggtttgcgc agctttgtgc ttcagttcct gatcgataaa ttcgttttcg cgcttaacgc
    16381 gggcttcttc tttcgccagc gccagttttt gtttctgaat ctctaagctg cgtagctcat
    16441 cttcataact ttgatcgcgt tttttgtccg cagtggcttc ggcgtccagt ttatcctgac
    16501 gagctttctt atttgccgct gccgttgccg ctcttttgtt cgcggcggcc tgggcatttg
    16561 cgcgacgttg cttctcttgc tggatttccc tgttgcgctc cgcgacccat tcgtcatgct
    16621 gcctttgctc ttcattttta ccttgctgtt ccgcttctgc gacagcagag agttgatcct
    16681 gcaatgatga ggctatagcc ggatagctta aggaggctaa gatggcgcaa agaaaaactt
    16741 tcttcatgac tcctcctgat tattagctct tttcaggaca tttggtattt ggctgaatac
    16801 gcgtttcgtt atacgttgtg gtaataacaa cggctaaacc tgtcgtaaac tggcactctt
    16861 taccctcctg ggtggaggta tacactttgg tgccttcctt atatgttaaa gaaacacctt
    16921 ccactaaggt tttatcattc accatagaac ccgctgccgc gcctacagct ccgccgccaa
    16981 ctgcccctgc cgtcgttccg gaattgctgc cagaaccgac gttgtggccg ataacaccgc
    17041 cagcgactgc gccaataagc gcgccgaagg cttgtgcgtt ccgtttattt tgggagttgt
    17101 ctacggcaac ttttgcggga agaatggaaa taatattaac ggttttagtt tcttgtttgg
    17161 tattcagttg atcggtttga taaacatcgg cggcatgatc gtcagcattt gactggcatc
    17221 ctgccagagt gaatgacgct aacattgcca caggcagaag acatttttta aatttcatca
    17281 ctattccttg ttatacacat tacgaaaaat aagacattta agatcatcaa aagaggaaag
    17341 taaatgtatt gcgtataaag ggatagatta aatttattaa aattcacgca gggaataatt
    17401 tataaaaata tatcattaca acatatatat taattttata tttatttcgc agccaaacag
    17461 gataaacatc agtcaggaca aagacgtaaa atttaagatg aaagaatatc tgccgctttg
    17521 ataatcccaa agagcaaaag catccgacag cgaagtgccg ttatgttctg tgaaagtgga
    17581 gaaggtaacc gctaacgttg taccggtgat tcgacggaga gtgtggattg ctgagcttct
    17641 tttttgctct gatggtgctg ccaggcaccg acggacgagt agataaaacg gccaaagaag
    17701 aagataaagc tgatgagcag tacgatacgg gtcatgcgac tgttaaatcg gtgtcgtttt
    17761 cgcatactag ttgcctgact cacaaaaggt tccttgaagt atgtcccacg ctgtggacgg
    17821 tacttacatt aaggcacaac aggacaaaat ggtcaattct tcgttatgta aaaaagcgtc
    17881 agtggataca tattttaatg ttatggaagt taatttaatt atttacaatg atgatgtaat
    17941 aatgatgaat taacatgata aacgtaataa ttcattaatc agggttattt tgtttgatat
    18001 atatcaattg gaactttcat atgacactta gaatcaatgc tctctatagt gatgaataat
    18061 catcattcga agtcaggtgg gatgcctgtc tgaatacacc tccttcagga tgtgggggat
    18121 tctgctgagc atctatgaaa ctgaatgcaa cttatataaa aatacgtgat aaatggtggg
    18181 ggcttccgct gttcctgcct tctttaatct tgcccatttt cgcccacatt aatactttcg
    18241 cgcatatttc ttccggtgag gtttttctct tttatctgcc tttggcactg atgatcagca
    18301 tgatgatgtt tttcagctgg gcggcattgc cagggatcac cttagggatt tttgtccgca
    18361 aatatgcaga gctgggtttt tacgaaacgc tctcattaac ggctaatttt attatcatta
    18421 tcattctctg ttggggcggt tacagggtct ttactccccg gcgtaacaac gtttcacatg
    18481 gtgatacccg tttaatttcc cagcgtatat tctggcagat agtgtttcct gcaacgctgt
    18541 ttctgatact tttccagttt gctgcatttg taggattact ggcgagcaga gaaaatctgg
    18601 tcggtgtcat gccttttaac ctcgggactt taatcaatta tcaggcctta ctggtgggta
    18661 atctgatcgg tgtcccgctg tgctacttca tcattcgggt agtgcgaaat ccattttatt
    18721 tacgtagcta ttattcgcaa ttaaaacagc aggttgatgc caaagtcacc aaaaaagagt
    18781 tcgcgctctg gctactggca ttaggtgctt tattgttgct gttatgcatg ccgttaaatg
    18841 aaaaaagcac aatttttagc accaattata ccttgtcatt attgctgccc ctgatgatgt
    18901 ggggagcaat gcgctatggt tataagctga tttcgctgct ctgggcggtc gtgttgatga
    18961 tcagcatcca cagctatcaa aattacattc ccatttatcc tggctatacc acgcagctga
    19021 ccataacctc ctccagttat ctggtattct cttttattgt caattatatg gctgtactgg
    19081 caactcgtca gcgagcggta gtcagacgca ttcagcggct tgcgtatgtg gacccggtgg
    19141 ttcatctgcc aaatgttcgc gccctga
//
LOCUS       DAJTRB010000083        17330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTRB010000083 DAJTRB010000000
VERSION     DAJTRB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.083"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..185
                     /locus_tag="OO330_003040"
     CDS             <1..185
                     /locus_tag="OO330_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5883824.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="OO330_003041"
     CDS             complement(210..401)
                     /locus_tag="OO330_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5883825.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="OO330_003042"
     CDS             complement(506..742)
                     /locus_tag="OO330_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5883826.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO330_003043"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OO330_003043"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5883827.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO330_003044"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OO330_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO330_003045"
                     /pseudo
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OO330_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="OO330_003046"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="OO330_003046"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5883828.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="OO330_003047"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="OO330_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5883829.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="OO330_003048"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="OO330_003048"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5883830.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="OO330_003049"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="OO330_003049"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5883831.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="OO330_003050"
     CDS             complement(10860..12233)
                     /locus_tag="OO330_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5883832.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="OO330_003051"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="OO330_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5883833.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="OO330_003052"
     CDS             complement(13344..13583)
                     /locus_tag="OO330_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883834.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="OO330_003053"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="OO330_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5883835.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="OO330_003054"
     CDS             13960..14247
                     /locus_tag="OO330_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5883836.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14917..15060
                     /locus_tag="OO330_003055"
     CDS             14917..15060
                     /locus_tag="OO330_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5883837.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15073..15282
                     /gene="cspE"
                     /locus_tag="OO330_003056"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15073..15282
                     /gene="cspE"
                     /locus_tag="OO330_003056"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5883838.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15448..16257
                     /gene="rlmA"
                     /locus_tag="OO330_003057"
     CDS             15448..16257
                     /gene="rlmA"
                     /locus_tag="OO330_003057"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5883839.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="OO330_003058"
     CDS             complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="OO330_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5883840.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4577 a         4178 c         4369 g         4206 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc tggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctagata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14401 aagatatttt cctcattaat ttattaggat gtttacatca gatttgtgat taagcgtggt
    14461 attatttatt agcgaaacgt ttctctcttg atttttttgc tcactcatca atttttctta
    14521 ttttaaattt acaatccctt tggggattga cttctcttta gggtaattga tagccgttaa
    14581 ctgactgttt tatgagaaaa agtgatataa ctttttattc attgcatagc agaaaatgtg
    14641 atattgcacg cactatgtaa taacttctcc ctctggcctg gaacaactga acttattgaa
    14701 ctatgttaga aactacgcca gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg
    14761 atatatcggt gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat
    14821 ttgtgcgtct gatcgagaca tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat
    14881 aaaagatttc gggttaaacg aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa
    14941 gtcctgagta aacacgttga cgttgaatac cgcttctctg ccgagcctta tattggtgcc
    15001 tcatgcagta atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa aacaatctaa
    15061 ggaatttttc aaatggcaaa gattaaaggt caggttaagt ggttcaacga gtctaaaggt
    15121 tttggcttca ttactccggc tgatggcagc aaagatgtgt tcgtacactt ctccgctatc
    15181 cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga aattcaggac
    15241 ggccagaaag gtccggcagc tgttaacgta acagctatct gatcgaatcc actgatctga
    15301 agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct ttttactatg
    15361 ctttatcttc gctcctggcg ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc
    15421 ggcctttagt atcctgccgg agttgtcatg tcgttttcct gtccactttg ccatcagcct
    15481 ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac atcagtttga tatggcgaaa
    15541 gaagggtatg tcaatctgct gcccgttcag cataaacggt ctcgtgatcc gggcgacagc
    15601 gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg gacattatca gccgctgcgt
    15661 gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa aggccacggc ggtgctggat
    15721 attggctgtg gtgaagggta ttacacacac gcatttgccg atgcgttgcc cgaaatcacc
    15781 acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag
    15841 gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt ccgataccag tatggacgcc
    15901 ataatacgta tttacgcgcc gtgtaaagca gaagaattag cacgagtagt gaagcccggc
    15961 ggctgggtca ttactgccac gccgggaccg cgacatttga tggagctgaa ggggctgatt
    16021 tacaatgaag tacatcttca tgcacctcat gcagaacaac tggaaggttt tacattacag
    16081 cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg atgaagccgt cgcattattg
    16141 cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa
    16201 gaagtgttcg actgccagac ggactttaat attcacctct ggcagcgttc ttattaaccg
    16261 tggaagtgcg tccagaggat ctggacgccg atgccgatca gcaccagccc gccgagaatt
    16321 tccgcttttt tcccaataat tgagccgata aagcgaccaa ccatcatccc taatgtcgac
    16381 ataatcaagg ttgcacaacc aatggccaat gcggtcgcga taatgttgac ctgaaggaaa
    16441 gcaagaccaa cacccacagc catggcatcc aggctggtgg caatcgcggt ggttaccagt
    16501 agccagaaac cgtgccgacg gcgcggctct tcatcttcat catctgcgcc acgaaaaccc
    16561 tcaataatca ttcgcccgcc gaggaatatc agcagcacaa acgcaatcca gtgattccat
    16621 tcaaggacaa accggctggc taacatgccc attccccagc cgatcagcgg cgtcagggtt
    16681 tcgacggcac caaaaataag gccggttcgc aatgcttcag aaaatttcgg tttatggagg
    16741 ggggcacctt taccgattga tgcagcaaat gcatccatcg acataccaaa cgcaagaaga
    16801 acagtagcag tgatattcat gacaacgtcc tgaccggggt atccataaaa cacatcatcg
    16861 cccccagcaa acatgcatac atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg
    16921 catagcgatt gatgtgcata tggtctcgcc tgactgaaag tcttcagtcc gtacgtgcca
    16981 cgttttgcaa cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc
    17041 aaatgagggc gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat
    17101 ttcattgctt tattgataac gattttcatt tatgtttata tatcaatgaa acgttaaaat
    17161 aaatgacaaa taagggaaga gataatatag cctaagctat gtctgattat taaaaataca
    17221 ctaaacatag ctttggctaa attcataagt tattgagttg atacgagggt aatgactcca
    17281 acttactgat agtgttttat gttcagataa tgcccgatga ccttgtcatg
//
LOCUS       DAJTRB010000084        17154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTRB010000084 DAJTRB010000000
VERSION     DAJTRB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.084"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO330_003059"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO330_003059"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5883841.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO330_003060"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO330_003060"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5883842.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO330_003061"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO330_003061"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5883843.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO330_003062"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO330_003062"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5883844.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="OO330_003063"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="OO330_003063"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR5883845.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="OO330_003064"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="OO330_003064"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5883846.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="OO330_003065"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="OO330_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="OO330_003066"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="OO330_003066"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5883847.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPPPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="OO330_003067"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="OO330_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5883848.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..10212)
                     /locus_tag="OO330_003068"
     CDS             complement(8974..10212)
                     /locus_tag="OO330_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5883849.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGVTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10433..10861
                     /gene="uspG"
                     /locus_tag="OO330_003069"
     CDS             10433..10861
                     /gene="uspG"
                     /locus_tag="OO330_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5883850.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10982..12547)
                     /gene="ahpF"
                     /locus_tag="OO330_003070"
     CDS             complement(10982..12547)
                     /gene="ahpF"
                     /locus_tag="OO330_003070"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5883851.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12792..13355)
                     /gene="ahpC"
                     /locus_tag="OO330_003071"
     CDS             complement(12792..13355)
                     /gene="ahpC"
                     /locus_tag="OO330_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5883852.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13727..14473
                     /gene="dsbG"
                     /locus_tag="OO330_003072"
     CDS             13727..14473
                     /gene="dsbG"
                     /locus_tag="OO330_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5883853.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14683..15585
                     /locus_tag="OO330_003073"
     CDS             14683..15585
                     /locus_tag="OO330_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5883854.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEYPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15732..15935
                     /locus_tag="OO330_003074"
                     /pseudo
     CDS             15732..15935
                     /locus_tag="OO330_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(15954..17101)
                     /locus_tag="OO330_003075"
     CDS             complement(join(15954..16864,16864..17101))
                     /locus_tag="OO330_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5883855.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         4232 a         4448 c         4142 g         4332 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactatg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcccccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaccccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gcggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa tgacgaacgg
     9961 aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt caacgacttc
    10021 ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt tgcctcgata
    10081 aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa tatcatctgc
    10141 ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc cgtgataagt
    10201 caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt aagggtagct
    10261 atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt gttcagtgag
    10321 aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata tcagggataa
    10381 atattataat catggagaat gatttgttca tgattaacag ggagaaaggg ttatgtataa
    10441 ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag ctgttcgcca
    10501 cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac tacccgggtc
    10561 agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag agcatctgca
    10621 acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg atccttcccg
    10681 cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt tggcagaaga
    10741 actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga cccatctgtt
    10801 aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg tcgttcgcta
    10861 acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt tttaatgcca
    10921 taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat cttacttctt
    10981 cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct ttggcacctt
    11041 cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc gctgcgaaca
    11101 cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg cggttacgtt
    11161 cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca aaaataccgg
    11221 ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga ccaacgactt
    11281 tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg acgtttttca
    11341 ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca aattccagca
    11401 gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg gagttaccgc
    11461 cgccgataac cgctacgcgt ttgcctttaa acagcgggcc gtcgcagtgc gggcagtagg
    11521 tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc cattttgcac
    11581 ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt tcaatctggt
    11641 gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg tcgatcacat
    11701 caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc ccttcagtct
    11761 tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca ccaaaacgtt
    11821 cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc gctgcacccg
    11881 ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc tcttctgccg
    11941 cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc atgcggccct
    12001 gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg cgatcggtga
    12061 tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg ttcaatacgc
    12121 tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc gagtaatagg
    12181 tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac tgcgcttctt
    12241 tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac tcgtggccca
    12301 gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc aggaaagacg
    12361 gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac agttctgcga
    12421 tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc gtggcaatta
    12481 actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc atatttgtgt
    12541 cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct gcggctacct
    12601 ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac ccgaagaatt
    12661 aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga atccccggga
    12721 gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat ggcgtgaaag
    12781 acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt tgcttcacct
    12841 tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg agctgctttg
    12901 attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc
    12961 tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc ttcatcttca
    13021 cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg
    13081 attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg agtatcggta
    13141 gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc agcaacgtca
    13201 cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa gacgctccag
    13261 cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt
    13321 ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt gttttcgatg
    13381 agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat caaatcgata
    13441 agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc agcggcgata
    13501 aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct ttggtatctt
    13561 cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac atggattaca
    13621 acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga cagtgatagg
    13681 ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt taaaaaagat
    13741 acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt ccccagtaaa
    13801 agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg gaggaatgaa
    13861 aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc ccgatggtaa
    13921 gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta acacacttat
    13981 cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg aacaatccca
    14041 ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct ccgatccgtt
    14101 ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt ctggcaaagt
    14161 gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga cagcagcggc
    14221 aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct ctggttgcaa
    14281 gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt taagtgacaa
    14341 tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt acatgagtaa
    14401 ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta atatcattat
    14461 gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt ttcaaccatc
    14521 ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta gaaaaactta
    14581 atcctgagtt aagccaaaaa acataatcaa taaaaataca tacgcaaaaa acattgatta
    14641 agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa tctctacgac
    14701 ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca gcatttaagc
    14761 attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca gtcgttacaa
    14821 cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg tatcgcgccg
    14881 acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg ccttgaacaa
    14941 acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta cggccctcag
    15001 ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga tgcctctgtc
    15061 gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact gttggttcac
    15121 cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt catttgcatg
    15181 ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg aattactgac
    15241 aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc caaatcagca
    15301 ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa aatttcattt
    15361 cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt actgggaata
    15421 gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga gataaaacta
    15481 gaatatccgt taccttcaat aaagctttac atttcctata ataaatcttc tttaaataat
    15541 ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat aactatagac
    15601 agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa cactctgctt
    15661 tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta acgttcagag
    15721 agtgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg gaagctgcac
    15781 gagagcgtat tacctagaca ctcaacaccc taccccgcgt ttgtgtctca ttttctggcg
    15841 gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa atgggcaaaa
    15901 aaatctgcgt tttgtttatc aactgggagg cgcaatgaat cgccacggat aatctagaca
    15961 cttccgagcc gttgataata ctggttttca tattctgtcg gtgacatctg ttcgctagaa
    16021 ccatgccgac gcttactgtt ataaaacatt tcgatgtaat caaaaatatc actgcgggct
    16081 tcttcccgcg ttccgtagat ctttttcttt atccgttcac gtttcaacaa ctggaaaaaa
    16141 ctttctgcaa ccgcattatc atggcagtta ccgcgacggc tcatgctacc ctccaggccg
    16201 tgtgatttca ggaacgactg ccactcatgg cttgtgtact gactgccctg atccgaatga
    16261 accagcacct gtttttcggg attacgccgc catacagcca tcagcagtgc gttcaggaca
    16321 atgtcctttg tcatccggga ttgcatggac cagccgataa ttttgcgtga gaacagatca
    16381 acaacaacgg caagatacag ccagccttcg tgggtcctga tgtaggttat gtccgttacc
    16441 caacgctcat caggagcatc cggattgaac tgtcgctgga gcctgttggg tgacacgata
    16501 ctggcctcgc ctttacgtgc ccgcgggctt cggtatccga cctgagcctt tattccgaca
    16561 cgtttcatca gtctccagac tctgtttact ccgcactgtt gcccgctgtc acgcagatcc
    16621 agatggattt tgcgataacc atagacgcat cccgattcca gccagaactg tttaatctgt
    16681 cctgtcagtc tcaggtctgc ctgatggcgt tgtgaatgcg gctgctgaag ccaggcgtaa
    16741 aaaccactgg gatgaacatc cagcacccga cagagcaggc gaacaggcca gcaacaggag
    16801 ttgtcacgga taaaggcgta cctcagtcgg acagctttgc gaagtacgcc gcggcttttt
    16861 ttaatatgtc ccgttcgtcg gtaacccgtt tcagctcttt ctggagacgg cggatctcgg
    16921 cctgagcatc tgactgttct ttattagtgg aagaatccgg accgtacttc tttatccagg
    16981 cataaaggct gtgggtggtg atatcgagac gtgttgcaac gctggcaaca gaataaccgc
    17041 gatcaacaac ctgtttgact gcttcagttt taaactcttc gggataacgc ttaccgctca
    17101 tgggcacctc tctttaagcc atcttaaatg actctgaggt gtctgttaaa cccg
//
LOCUS       DAJTRB010000085        17081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTRB010000085 DAJTRB010000000
VERSION     DAJTRB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.085"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..509
                     /locus_tag="OO330_003076"
     CDS             <1..509
                     /locus_tag="OO330_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5883856.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(510..1670)
                     /gene="ybdL"
                     /locus_tag="OO330_003077"
     CDS             complement(510..1670)
                     /gene="ybdL"
                     /locus_tag="OO330_003077"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5883857.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAILLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1779..2867
                     /gene="hcxA"
                     /locus_tag="OO330_003078"
     CDS             1779..2867
                     /gene="hcxA"
                     /locus_tag="OO330_003078"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5883858.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2877..3074)
                     /locus_tag="OO330_003079"
     CDS             complement(2877..3074)
                     /locus_tag="OO330_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5883859.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3256..5361)
                     /gene="cstA"
                     /locus_tag="OO330_003080"
     CDS             complement(3256..5361)
                     /gene="cstA"
                     /locus_tag="OO330_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5883860.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5542..5955)
                     /gene="entH"
                     /locus_tag="OO330_003081"
     CDS             complement(5542..5955)
                     /gene="entH"
                     /locus_tag="OO330_003081"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5883861.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5958..6704)
                     /gene="entA"
                     /locus_tag="OO330_003082"
     CDS             complement(5958..6704)
                     /gene="entA"
                     /locus_tag="OO330_003082"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5883862.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSLGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6704..7561)
                     /gene="entB"
                     /locus_tag="OO330_003083"
     CDS             complement(6704..7561)
                     /gene="entB"
                     /locus_tag="OO330_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5883863.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7575..9185)
                     /gene="entE"
                     /locus_tag="OO330_003084"
     CDS             complement(7575..9185)
                     /gene="entE"
                     /locus_tag="OO330_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5883864.1"
                     /translation="MSILFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9195..10370)
                     /gene="entC"
                     /locus_tag="OO330_003085"
                     /pseudo
     CDS             complement(9195..10370)
                     /gene="entC"
                     /locus_tag="OO330_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10662..11618
                     /gene="fepB"
                     /locus_tag="OO330_003086"
     CDS             10662..11618
                     /gene="fepB"
                     /locus_tag="OO330_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5883865.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITEHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11622..12854)
                     /gene="entS"
                     /locus_tag="OO330_003087"
     CDS             complement(11622..12854)
                     /gene="entS"
                     /locus_tag="OO330_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5883866.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12965..13969
                     /gene="fepD"
                     /locus_tag="OO330_003088"
     CDS             12965..13969
                     /gene="fepD"
                     /locus_tag="OO330_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5883867.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13966..14958
                     /gene="fepG"
                     /locus_tag="OO330_003089"
     CDS             13966..14958
                     /gene="fepG"
                     /locus_tag="OO330_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5883868.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMCLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14955..15770
                     /gene="fepC"
                     /locus_tag="OO330_003090"
     CDS             14955..15770
                     /gene="fepC"
                     /locus_tag="OO330_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5883869.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15767..16900)
                     /gene="fepE"
                     /locus_tag="OO330_003091"
                     /pseudo
     CDS             complement(15767..16900)
                     /gene="fepE"
                     /locus_tag="OO330_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3990 a         4865 c         4443 g         3783 t
ORIGIN      
        1 cctttcgtga aaagccagtg gattgcgttc tgtgggttaa aaacagccaa cttatgccta
       61 atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa actgctgcaa aaatccattg
      121 aaatagatgg ttttacccaa cccattgtgg tcacacatac ggataaaaat gccatggaaa
      181 tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc atcatcatta aaattacgct
      241 taaaaggtta tttgccggtg acctgtctgg aaggtacgcg taatcaacgc atcgccgcga
      301 ctattcgtca taatcgcgct cgtggacgcc atcaaatcac cgccatgtcg gaaatcgtcc
      361 gtgaacttag ccagttggga tgggatgata ataaaattgg caaagagctg ggaatggaca
      421 gcgacgaagt gctgcgactg aagcaaatta acggcctgca agagttgttt gccgaccgtc
      481 aatattcccg cgcctggacg gttaaatagc taaagctggc gcaggcgttc agctgctgcc
      541 agcaacgtcg attccttctt ggcaaaacag agacgaatca gtttatgtgg gaagggatcg
      601 gcgcaaaaca ccgacagcag aatcgccgct acgccgtgct cctgcgtcag ccactggcaa
      661 aactcaacat catccagggt agaaaccgcg ctgtaatcca ccagcaaaaa gtatgtacct
      721 tcacacggta aaatctccag ccggctttca tttaaagcat tcaccagaat atcgcgcttc
      781 tggcgataaa agtccggtaa cgcaagataa tgctcaggtt ctgcacgtag catatcagca
      841 agcgccagct gtgccggggt attcaccgaa aaggtcagat actgatgtac cttgcgaatt
      901 tcggcgctga tgggcgctgg cgcaacacaa taacccactt tccagccggt catatgatag
      961 gtcttgccaa atgaagaaac cgccactgcc cgctcacgca gctgcggatg cgccagcaca
     1021 ctggcatggc cctgttgtga aaagttgatg tgctcgtaga cttcatcgct aatgacaaaa
     1081 atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat cagcctgctg ccagacagtt
     1141 gcactggggt tatgcggagt gttgaggatc accagtctgg tgcgctcgct taataatgcg
     1201 gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca gtgccatacg cttcactatt
     1261 cccccagaaa gcgcgatggc gggggcgtaa ctgtcatagc tgggatcaaa acaaatcact
     1321 tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata acgcttccgt cgcccctgcc
     1381 gttacggtga tatcgctatc ggcatctggt tgatagccat acaaacgttc cgttttctga
     1441 gcaatcgcct cgcgcaaggc ctgcacgccg gtcatgggcg cgtattggtt tgccccctgg
     1501 gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac catcaaaatc aggaaagcct
     1561 tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc tcatctgggt gaaaatagtg
     1621 gtgccaagtt gtggaagttt gctttgtgga atcagagggt tatttgtcat ttttattgta
     1681 cctgacgaag ttgcgtgttg aaggcactat aacacgatgc cagtatttgg caatcaagac
     1741 gtttagatgt ctaaataaaa caataaggac aacacaacat gcctcacaat cctatccgcg
     1801 tggtcgtcgg cccggctaac tacttttcac atccaggaag tttcaatcac ctgcacaatt
     1861 ttttcactga tgaacaactt tctcgcgcgg tgtggatcta cggcaaacgc gccattgctg
     1921 cggcgcaaac caaacttccg ccagcgtttg gacttccagg ggcaaagcat attttgtttc
     1981 gtggtcattg cagcgaaagc gatgtacagc aactggcggc tgagtccggt gacgatcgca
     2041 gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac cgcgaaagcc ctcgcccgcc
     2101 gtctcggtct gccatttgtt gccgctccga cgatcgccgc cacctgcgca gcctggacac
     2161 cgctctccgt ctggtataac gatgccggac aggcgctgca ttatgagatt ttcgacgacg
     2221 ccaattttat ggtgctggtg gaaccggaga ttatcctcaa tgcaccgcaa caatatctgc
     2281 tggcggggat cggtgacacg ctggcgaaat ggtatgaagc ggtggtgctg gctccgcaac
     2341 cagaaacgtt gccgctaacc gtgcggctgg ggatcaataa tgcgcaggcc attcgcgacg
     2401 tcttgttaaa cagtagcgaa caggcacttg ccgatcagca aaatcagcag ttaacgcaat
     2461 cattttgcga tgtggtggat gctattattg ctggtggtgg gatggtcggt ggtctgggcg
     2521 atcgttttac gcgtgtggcg gcggctcatg ccgtgcataa cggtctgact gtcctgccgc
     2581 aaaccgagaa gtttcttcac ggcaccaaag tcgcttacgg aattctggtg caaagcgcct
     2641 tgctgggtca ggatgatgtg ctggcgcaat taaccggggc gtatcagcgt tttcatctgc
     2701 cgactacgct ggcggagctg gaggtggata tcaataatca ggcggagatc gacaaagtga
     2761 ttgcccacac cttgcgcccg gtggagtcca ttcattacct gccagtcacg ctgacaccag
     2821 atacgttgcg tgcagcgttc gaaaaagtgg aatcgtttaa agcctgacgt gcaaatttag
     2881 cagcagcgcg cgccgccttt tccaccgtag cgcgcgtcct gccgctcacg gaaaaactcc
     2941 tcgtaggtca tcggcgtttg atcgggatga ttaacccgca tatgttcgac atagttgtcg
     3001 taatcaggca taccaatcat cagcttcgcc gcctgaccta aatattttcc ggcttttgcc
     3061 agtgaatcaa acatcttggc ctccgctaac catgttgctg aaagagtgcc ggatgcggcg
     3121 tgaacgcttt atccggccta caaatgctat caacgcactc ggtcagtccc ctcgccccat
     3181 cggggagagg gttagggtga gggggaaccc tcggcactat gccaacattc gccaacatcc
     3241 cccctcactc tgactttagt gcgcgccttt cgcctgcgct acgatctcct cgacattttc
     3301 cggcatcggc tcatacggcg tttctttcgc cgttggcttc ggatctttca atgccgcaag
     3361 tgccgtctta atcgagaaca gtgccaggac caccacgacc accataaaga agatggttaa
     3421 cccggcatcc agacggttgt tgaacaccag ttgcgccagc tgtgactcag tatactgcga
     3481 cggaatattg ccgctgtcga tcattgcctg gaacttgtta gcaatggcca ggaagccgac
     3541 tttcgcatcc gggctaaacg ctttctgcca gtcagcggtc agggtacaaa tcagcagcca
     3601 ggccgttggt accagcgcca cccaggcgta acgttgacgt ttcatcttga acaacaccac
     3661 ggcacagagc atcagcgcca tccctgccag catctggttg gcaataccaa acagcggcca
     3721 cagagtgtta ataccgccca gcggatcgac cacgctctga tggaggaagt agccccacgc
     3781 cagtacgcac agcgctgttg ccagcaggtt agcaggcagt gaatcggtcc gcttcaggcc
     3841 aggagacacc acgcccagca gatcctgcaa cataaagcgc gcagcacgcg tacccgcatc
     3901 taccgccgtc agaataaaca gagcttcaaa cagaatggcg aagtgatacc agaacgccac
     3961 atccatcata ccacccagcg caccgtgcag gatgtaagcc atccccaccg ccaacgtcgg
     4021 cgcaccgccc gcacgggaaa tgatcgactg ttcacccact tcgctggcaa tctggttgag
     4081 cgtatctggt gtaatgctaa agccccagct actcaccacc tgcgcggcag aagcgactac
     4141 atccgccgtc cctgccggag ccagcaccgc cattgggctg ttcatggcaa aatacacgcc
     4201 cggatcgatg atacaggcag aaactagcgc cataatcgcc acgaaggatt ccatcaacat
     4261 cccaccgtag ccgataaagc acgcctgccc ttcgttcgcc agcatcttcg gcgtggtgcc
     4321 ggaagagatc agcgcatgga agccagacac cgcaccacag gcgatggtga taaacaggaa
     4381 cgggaacagg ttaccggtcc ataccgggcc agtgccatcg acaaatttgg tcagcgcagg
     4441 catggtcagc gtcgggcgca taatcaaaat gcctaccgcc agaccgacga tagtcccgat
     4501 tttcaggaag gtcgagagat agtcacgcgg ggccagcagc aaccacaccg gcagcaccgc
     4561 cgccacaaaa ccgtagccca ccagcatcca ggtcagctgc acgccggtaa agtcaaagta
     4621 cggtgcccag gtcggacttt ctgccaccca gccgccagag ataatggcga aaatcaggaa
     4681 caccaggcca atcactgaca cttcgccaat gcggcccgga cgcagatagc gcaggtagat
     4741 tcccataaaa atcgccagcg gaatggtgaa cgcaacggta tacgttcccc acgggctatg
     4801 agtcagggct ttcaccacga tcatcgccag tactgcaagg ataatgacca tgatcataaa
     4861 gcaggctacc agcgcaatca ccccggcagt tggccccatc tcttctttga ccagctcacc
     4921 cagcgagcga ccgtcacggc gcgtagaaac aaacagcacc atgaaatcct gcaccgcacc
     4981 ggcgagaacc accccggcga gcagccagat catccccggc aggtagccca tttgcgctgc
     5041 cagcaccggc cccaccagcg gccctgctcc ggcaatggcc gcaaaatggt gaccgaacag
     5101 cactttcttg tccgtcggca catagtccag cccgtcgtta tggcgcaccg ctggcgtcat
     5161 acgcgtcggg tcaaccgcca gcacattttt ggcgatatac agcccataaa aacggtaagc
     5221 gatcagatag atacagaccg acgccaccac aatccacagc gcgttgatct gttccccacg
     5281 atttaaagca atgtatccca gagcaaatgc tcccattaca gagagcactg tccagacgag
     5341 gtatttccct gatttgttca tagttgttat ccgtgtgcgt gtccatagag atgttacatt
     5401 ttgtttctat aacatctttg tacgatttaa caacacggta accatgtaaa tcggagttga
     5461 aaccaatatt taacttaaca atgttaactc gatcactccg ttaaccgagt tttcctgcaa
     5521 tctcatttaa ttctgtcggg ctcatcccaa aactgccgta cccagccgac aagtgcagca
     5581 acgccgcccc tgttcatcga aaacgacgat ttcccagctc tgattttgcc gcccaagatg
     5641 cagcggctgg cagacgccgc gtaccttgcc ttcagacacc gggcgatgat gggtggcgtt
     5701 aagttccgtg cccaccacac actgcccgtc acgggtcatc ataaatccgg ccatcgatcc
     5761 cagcgtttca gccagcgccg ccgacgcgcc gccatgcagc aggccaaacg gctgatgagt
     5821 acgggtatca accggcattt cggcttccag cacatcatct cccagacggg tatacacaat
     5881 tcccagatgc gccaccattg tgttatcgct ggtggcgttc agttcgtcga gcgttaaatg
     5941 gcgtttccag atcatgctta tgcccccagc gttgagccgc catcgaccac aatatcctgt
     6001 agggtgatat ggctggcgag gtcagaggcg aggaacaaaa tcgtgttggc gatctcttgt
     6061 ggacgggcga ttttccccag cggaatgccg agtttaaact gctcgccaaa gccgcgaata
     6121 cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt gcatatcggt gtcggtggag
     6181 ccaagcgaaa ccacattaca gcgcacgccg ctacccgcca gttccagccc gacgctcaac
     6241 gccaggcttt tcagcgccgc tttcgatgcg ccataagcac tcataccaat acgcgccgtg
     6301 tgtgcggcgt cggacgccac agtgacaatc gccccgcccc gctgacggcg aaactggttc
     6361 atggtttgct ggaacaggtt aaacgcaccg ccgacgttaa ccgcaaaagt ctgctgccag
     6421 tcctctttac tgagctgatc ggtcgcgccc atgcgcaaaa ttcccgccgc attgatcagc
     6481 acgtccagtc gctccgtttc agctaacagt cgctgacata cctgcgccac ctgcccggcg
     6541 tcggcaacat ccattacttc ggtcgcaaag ggatattgct cctgagtgaa cgcttgatca
     6601 aaacctgtaa ctttcgctcc cgcctcaaca aacgccagcg ccgtggcgta gccgatacct
     6661 ttacctgcgc cggttaccca gacattttta ccgctgaaat ccattatttc acctcgcggg
     6721 agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc cagcatgaca aagtcgatat
     6781 caccatgcac tttgcgccag cgcgccgcca gcgccatcat gcgcaccgaa tccagaccgt
     6841 agtcgatcag gttgtcgtca tcgaacggtt catcggactc gtccagcaac ggcaggatca
     6901 cctcacgcag cgccgctttg ctggcgggga taggtgctgg cagtaattct tcggtcatca
     6961 ccacccggcc agaacgtccg gccacatatt tcagcgacat caaatgctcg tcacggctga
     7021 aatcagccag cgcatccgcc accataaacg gtttaatatc gcgcataaat gcgtcggttg
     7081 cggtggtcat acagccaatg tgggcatata ctcctgtaat aatcagctgg ttacgtccac
     7141 tctctttgag catttgctcc agcggagaac gatgaaacgc gctgtagcgc cacttcacca
     7201 gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac cttttgctgt tccggcgagc
     7261 gggtcaggcc cggcccccac atatcattca acagcgcccg atcttcatcg ctctgctctt
     7321 tcggctgggc ggtgtaataa accgggatat tgtgctgttt gcagtagtcg cgcagcgcag
     7381 caatattcgc gatcacctgc tccatcatcg ggcagttctc gccccagaag ctgacaaaat
     7441 agtcctgcat atcatggatt aacaacgcgg cacgttgcgg ttcaaaggcc cagtcaactt
     7501 tattctgcgg aatatcgtga gactccggca gtgcgtaagc ctgtaatttt ggaatagcca
     7561 tcgtgttctc tccttcaggc tgatgcgcgt gacgccagcc actgacgtaa ttgtttttta
     7621 tcgactttcc cgaccgccgt cagcggaagt gaatccacac actccacgcg atccggtaat
     7681 ttaaattcgg caataccctg ttcacgcagg aaacgacgca cctgcaccgc gcgcagcggc
     7741 tcttttacca ccagataagc acagcttttt tcgcccatca gctcattttc catgctcacc
     7801 agtgcggcat agatcactgc cggatggctc agcagcaggt tttcgatatc ttcggcagcg
     7861 atcttctcgc cgccacggtt gatctgatct ttctcgcgcc cctgcacggt gatgtacccc
     7921 tctggatcaa tagagatcag atcgccggaa cagtaaaaac cgttggcatc aaaggcactg
     7981 gcattgtgct gcggactttt gtaatagcca cggaaggtgt acggcccgcg cgtcatcagg
     8041 cgtccaactt ccccttgcgg cagtggattt ccttcggcat cggcaaccca cacttcatcg
     8101 tccggacaca ttgggtaacc ctgggtatgg ataattttct ccgcgctatc atcaagacgg
     8161 gtgtagttca ccagcccttc cgccatgcca aacacctgct gcaactggca gccaatctca
     8221 gcgggaatac gcgctgcaag cgtggcagaa agacgtgcgc cgccgacctg taacagtttc
     8281 agcgaggcaa gctgcgcccg gctttcgcct tcagccagcg cctgcaacca caggctgact
     8341 gccggcggca ccagcgcggt aacgttaacc tgatgttttg caatcaacgg gaagcaaagc
     8401 gtggcgctgg gatcggccgc cagaacaacc gttcctccgg caagaaagac gcccagcgat
     8461 cctggcgaac tcatagcgta gttatgagcc gccgggatcg cgcacaggta gcgcgtctgt
     8521 tgtgtgaact gacaaatctc ggcgctacga cgcacgctgt agtaatagtc gttatgagtg
     8581 cgcgggatca gtttcggtgt gccggtggtg ccgccggaaa gctggaaata ggccacttca
     8641 tcagcaggtg atggcgtggc agtaaaaccc tctgccggat ggttaatcgc atcctgcaag
     8701 ttatgctcac cgctgtcgtt gtgcagttgc accacgcgaa tggaagaatg ctctgcaaca
     8761 aatgtgttga ggaaatcatc cccgctaaat agcgcatgct ggcgatcggc aatcagtaac
     8821 gccggttcaa tctggctggc gtaggcgttc agttcactac gctgatggct gaacagcgcc
     8881 agcaccggcg cgacgcccag tttcagcagc gcgaaaaagg taatgtaaag ttcagcgacg
     8941 ttacccagtt gtaccagcgc ggtttcacca ggtttaatgc cctgacggcg caaactacag
     9001 gcgaggttat ccgccgcctg attcagctcc cgataactca actgccgctc gccgtcgata
     9061 accgcgatgc tgtcactcgc agcgtggcga gtcagaatgt cggtcagtgg caaatcctgc
     9121 cagtagcctt tttcccgata gcgacgggca aactcttccg gccagcgggt gaatagaatg
     9181 ctcatcctcg ctccttaatg taatccaaaa acgttcaaca tggtagaaag tttgacgcct
     9241 gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa tccccgctcc ggcaaacaga
     9301 cgcacctgat tttcccgcag cttcgcgcag cggatggtca ccacccattc gccgttacct
     9361 tcgctgtcac accaacccac aatgccgcca aacagttcgc ggtcgaacgg ctccagttca
     9421 gcaataacct gggtcgcggc ctgatgcggg aaaccgctca gcgcgggggt cggatgcagc
     9481 agacaggcca gagtcagtgc gttttcttgc gaattcgctt taccttcaaa gggagttgcg
     9541 agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg agggaacgtg taactcacta
     9601 ctgcgttcgc gcagtacctc tttcatcgcc tgagtcacca gttcatgttc atggcgatct
     9661 ttttctgacg ccagcagacg attacccgct tcgcgatcga gcacttcatc cggctgacga
     9721 cgcgcggaac cggctaacgg aatggagcta aaacgctcgc cgtctttacg tagcagcagt
     9781 tccgggctgg cccccagcag gacgccacca tcagccagcg ggacatggaa gttgtaacta
     9841 accgggtttt gcgcaatcaa ccgttccagc aatacgccac tatcaatggc ggcgtcagtg
     9901 gtgatatcaa tcaatcgtga caacaccact ttgtcgacct gcggcgtggc ggtaagtgcg
     9961 gcggcgcggg cacccatctg ttcaaacgtg gtttgttcag gaattgcctg gcgttccacc
    10021 acgttcagcg actggctgcg ggtaaaacag cgggctgagg tctgtttttc ctgacgggag
    10081 aacgactgct aggattcggg aatatacagc gacgaaggct gacgtggatc gaagggaatc
    10141 gccccgacca tcaccggatt tttgatgccc tgcgctttgg catcggcaaa cagcgcggcg
    10201 agtttttgct ggaagggact gtcgggcgaa tccccgttca cagccggttc atcgaaacgg
    10261 gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca taaagaaaaa gcgattgggc
    10321 gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg acgtatccat atcatcctcc
    10381 acaaaatgat aaaggcttta ataatgatta tcacttatat tttcggccgc taacctaaaa
    10441 gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct gcaactcggg gttgcatctg
    10501 gttgtggtta caatgaaaat gagaagcatt attgatgaat tcgcataagc gcaatgtgat
    10561 gtcctgcgcc gttttgcccc ctctccccaa agggacggat tgtgcgcttt gtcgaatttg
    10621 tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc gccccgctct
    10681 accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta gttcaggccg
    10741 ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa agccagccgc
    10801 agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt gatgctccgg
    10861 tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag ggctttttac
    10921 gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc ggcgaaccga
    10981 gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca accggcgggg
    11041 attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta atcatcaatt
    11101 acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc gagcatgaga
    11161 aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg aaagagcaaa
    11221 tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca cacagtgcca
    11281 atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc tttacgctgg
    11341 cgaaattgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat gacatcattc
    11401 agcttggtgg ggaaaatctg gctgcggggt taaacggtga atcgctattc ctgttcgctg
    11461 gtgatcagaa agatgccgat gcgatttatg ccaatccact gctcgcgcac ctgcctgcgg
    11521 tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt ccgcctggat tactacagcg
    11581 ccatgcaagt gctggaacgg cttaaggcgc tgttttaagc attaactgtc ggacgctgtc
    11641 acctgcggcg gcgtctggcg aaaacgtcgc aactccacca gcaccagcaa taacaacacg
    11701 ccgataatca acaaaccaaa accgctcgcg cttgcggaag caaccggtgt catcatcgcg
    11761 cccaaaccac ccagcagcgc cgcgcctatg gcatcgcccg tcacgttctg cgccgtccac
    11821 aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg tttgcagcat tgtatattgc
    11881 agcaacgaac taaccgcgct cagccagccg aacagcgcca gacaaaccac gcctaaaatc
    11941 cacatcggca tcaggccaaa cagaccaatg gcgaggaacg atcccagcgt ggagagcagc
    12001 atcaataacc ctggtcgcgc actatgtgcc agcttcccgc tggttaacgc accaatagcc
    12061 gcgccgagcg ggatcgccgc gtagagaaaa ccaatctgtg ccgctgacat ctgccagttg
    12121 tcagccagcg ccggatacag taccagcacc gcgctcgcca tcgtcaataa accacccagc
    12181 agcgggctgg caagcagaaa acgaaatcct gccagtaatg atttcaacgg atgctcaagc
    12241 ggctgcggtg gcggtggcaa cgccggaagg cttaataacg gtaacaaggt aataaacgtg
    12301 cccgccgccg ccagcccgta gttccaggct acgccgccgg tcgccagcaa taaaccgcca
    12361 atcatgggcg aaatcaccga ccccaggcgc acggtcaaca tggtgatcgc cccggcctgc
    12421 attaagtttt cacgccctac cagtgcagat gttgccgcca gtagcgccgt aacaccaagt
    12481 gatgcgaaaa aaccatccca taaaccaagt aaatagattg ccagcaatga cggctccggc
    12541 agcagtgcat taaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa
    12601 atcacttttt tgcgttcata gcgatctgcc agcacaccgc caaccatcag gccaacaaac
    12661 atcgcgccgc cggtcagcgt caccgaaagc cccacctgcc aggtagaatg ggtcatcatc
    12721 tggatctgca ccggcaccgc gacgccgagc aaacccagag acacaattga gataaaacga
    12781 gcgaggaata ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag
    12841 gattgtttat tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg
    12901 cacatgttaa catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt
    12961 tcgtatgtct ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt
    13021 actgattatc gcgacggcat taagcctgct cattggggca aaatcactcc ccgcttccgt
    13081 agtgctggag gccttctccg gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc
    13141 gcggctgccg cgtacccttg ccggtttact ggcaggcggc gcgcttggcc ttgccggggc
    13201 gttaatgcaa accctcaccc gaaacccact tgccgacccc ggcttgcttg gcgtgaacgc
    13261 cggagccagc tttgccattg tgctgggtgc ggcgctgttt ggttactcat ccgcacagga
    13321 acaactggcg atggccttcg ccggggcgct ggtggcctca ttgattgttg cctttaccgg
    13381 cagtcagggt ggagggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc
    13441 ggcggtgctg gaaggactga ccagcggcat cgccctgctt aatcctgacg tctacgatca
    13501 actgcgtttc tggcaagccg gttcgctgga tattcgcaat ctacatacct taaaagtggt
    13561 gctgatcccg gttctgatcg ccggagcaac tgcgctatta ctgagtcgcg cgctgaacag
    13621 tttgagcctc ggcagtgaca ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt
    13681 gattggtctg ctggcgatta ccgtgctttg tggtagtgcg acggcggtag ttggcccgat
    13741 tgcctttatc ggcctgatga tgccgcacat ggcgcgctgg ctggtaggtg ccgatcatcg
    13801 ctggtcgctg cccgtcacgc tacttgctac ccctgccctg ctgctgtttg ccgatatcat
    13861 cgggcgggtg attgtgcccg gcgaactgcg cgtttctgtg gtcagtgcgt ttattggtgc
    13921 accggtgctg atcttcctcg tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct
    13981 cgccgattac tcatcacctg tttgctgctg atttccgcct gtgtggttgc aggtatctgg
    14041 ggattacgca gcggtgccgt cacgctggaa atctcgcagg tattcgccgc gttgatgggc
    14101 gatgcgccgc gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg
    14161 gcgctgttga ttggcgcggc tctgggcgtc agcggcgcga tttttcagtc gctgatgcgt
    14221 aacccgctcg gcagccctga cgtaatgggc tttaacaccg gggcgtggag cggcgtgctg
    14281 gtggcgatgg tgctgtttgg tcaggatctg acggctatcg cgctggcagc aatggtgggc
    14341 ggcattatca cttcgctgtt ggtctggctg ctcgcctggc gtaacggtat cgacaccttt
    14401 cggttgatta ttatcggtat cggcgttcgc gccatgctgg tggcctttaa tacctggctg
    14461 ttgctgaaag cgtctttaga aacggcgcta acagcaggtt tgtggaatgc cggatcgctc
    14521 aacggcctga cgtgggcaaa aacctcgcct tccgcaccca tcattatatt gatgctcatt
    14581 gccgccacct tgctggtgcg gcgaatgtgc ttgctggaga tgggcgatga taccgcctgc
    14641 gcgctgggcg tcagcgtcga gcgttcgcgt ctgttaatga tgctggttgc agtggtgctt
    14701 accgctgcgg caacggcgct tgccgggccg atttccttta ttgctttagt cgcgccgcac
    14761 attgcccgac gcattagcgg caccgctcgc tgggggctga cccaggcggc gctgtgcgga
    14821 gcgctgttac tgctggcggc cgatctctgc gcccaacaac tgtttatgcc gtatcaactt
    14881 ccggttggcg tcgttaccgt cagcctcggc ggtatttacc ttatcgtctt gttaattcag
    14941 gagtctcgca aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctgg
    15001 gatatggcaa atataccgtt gcggaaaatc tgactgtaga aattcctgat ggtcacttca
    15061 cggcaattat cggaccaaat ggctgtggta aatccacgtt actgcgtacc ttaagccgcc
    15121 tgatgacgcc tgctcacggg catgtctggc tggatggcga gcacattcaa cattacgcca
    15181 gtaaagaagt tgcacgccgg attggtctgt tggcgcaaaa tgctaccacg ccgggcgata
    15241 tcaccgtgca ggagctggtg gcgcgtggac gttatccgca tcaaccgctg tttacccgct
    15301 ggcgcaaaga ggatgaagaa gcggtaacga aagcgatgca ggccacggga ataactcatc
    15361 tggcagatca aagcgtggat accctttctg gcggacaacg ccagcgagcg tggatcgcga
    15421 tggtgctggc ccaggaaacg gcaattatgc tgctcgacga acccacgacc tggctggata
    15481 tcagtcatca gattgatcta ctggaactgt taagcgaact gaaccgcgag aaaggctata
    15541 ccctggcggc ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg
    15601 cattgcggga agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac
    15661 tgattgagcg catttatggt ctgcgatgca tgatcattga cgatccggta gccggaatgc
    15721 cgcttgtggt gccgctcgga cgaacggcac cttcaaccgc aaatagttaa actaagtggt
    15781 ctgccatcat ggcatcctgt tttctggatg ccatcgcata gcgcaataac acgctaccac
    15841 aagccaccat cccgccgatc agcgcggaaa ggatcacaat aatcgcctta cccggaccgt
    15901 cttttttcac tggcaatgac gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt
    15961 tgacatttgc ttttgttaat tgctcgacaa gatactgccg attacgtaat tcaccgttca
    16021 gttccgcaac gtcagtaacc gctttttcaa tttccagttt gcgttcaata ccgtctgcgc
    16081 cgagagaaat agagaaatcg gggtcatctt taacggcctg accattactg tatacgggct
    16141 ttttaattcc tgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat
    16201 caagttgatt tttcgtttta atgcgatcct gagccagttt ttctttttca aactgggttt
    16261 tgatctccag tttattacgg acgttttcta tcgactcttt caccaccaac gcagagatat
    16321 aatcgatata cccgctcaaa acggtctgcg cctctttact ggttggcgcg gtaaaactta
    16381 gcgtccagga ggtatacagt gacggttcat cttttttctt actagcattg tcatcaaccg
    16441 ctttcatttt ttcgctcaat gcaacaatag cgcgatgcaa atccagttca tcgattttcg
    16501 cctcttttaa ttagtccatc acataaggtg atgaacgcag gtactcttcc agcaagctaa
    16561 cagactgaaa cttcttgata aacaggttaa atgcaccgcc acggtcgata ttaacatcga
    16621 gatcaagaac gcggagtttg gtaaaggttt tctccaggtc ctgccactga atggcttcgg
    16681 cgggagtaat aacggctgag ctggtccatt tttgcggcag gatgaaagag atcagtaaac
    16741 ctgcgcaggc aaatgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactt
    16801 cgattagatt aagtaaatca atttcattat tactgggcga cgccagagga tactcagaaa
    16861 aatgagcgtc acttccctgt ttaatattca atgatgacat ggggtataac ctgaaaatga
    16921 gtccaatagg ggaaaatctc cacggcgctc actttaccag ttggtgagaa ttatccgaag
    16981 ctgaagtttg taaattcctt ccactgaatg caacattcac tctatttaag agaaatattc
    17041 atcaaaccta taaatcaaac aaataaaaaa cccataatta t
//
LOCUS       DAJTRB010000086        16392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTRB010000086 DAJTRB010000000
VERSION     DAJTRB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.086"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(90..2066)
                     /gene="speA"
                     /locus_tag="OO330_003092"
     CDS             complement(90..2066)
                     /gene="speA"
                     /locus_tag="OO330_003092"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5883870.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="OO330_003093"
     CDS             complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="OO330_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5883871.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2342..2557
                     /locus_tag="OO330_003094"
     CDS             2342..2557
                     /locus_tag="OO330_003094"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883872.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2861..4015
                     /gene="metK"
                     /locus_tag="OO330_003095"
     CDS             2861..4015
                     /gene="metK"
                     /locus_tag="OO330_003095"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5883873.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4452..5846
                     /gene="galP"
                     /locus_tag="OO330_003096"
     CDS             4452..5846
                     /gene="galP"
                     /locus_tag="OO330_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5883874.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5923..6420
                     /locus_tag="OO330_003097"
     CDS             5923..6420
                     /locus_tag="OO330_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5883875.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6515..7222
                     /gene="endA"
                     /locus_tag="OO330_003098"
     CDS             6515..7222
                     /gene="endA"
                     /locus_tag="OO330_003098"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5883876.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7302..8033
                     /gene="rsmE"
                     /locus_tag="OO330_003099"
     CDS             7302..8033
                     /gene="rsmE"
                     /locus_tag="OO330_003099"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5883877.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8046..8996
                     /gene="gshB"
                     /locus_tag="OO330_003100"
     CDS             8046..8996
                     /gene="gshB"
                     /locus_tag="OO330_003100"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5883878.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9105..9668
                     /locus_tag="OO330_003101"
     CDS             9105..9668
                     /locus_tag="OO330_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5883879.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9668..10084
                     /gene="ruvX"
                     /locus_tag="OO330_003102"
     CDS             9668..10084
                     /gene="ruvX"
                     /locus_tag="OO330_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5883880.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10260..11240)
                     /locus_tag="OO330_003103"
     CDS             complement(10260..11240)
                     /locus_tag="OO330_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5883881.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11258..11962
                     /gene="yggS"
                     /locus_tag="OO330_003104"
     CDS             11258..11962
                     /gene="yggS"
                     /locus_tag="OO330_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5883882.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRLAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11980..12546
                     /gene="yggT"
                     /locus_tag="OO330_003105"
     CDS             11980..12546
                     /gene="yggT"
                     /locus_tag="OO330_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5883883.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12543..12833
                     /locus_tag="OO330_003106"
     CDS             12543..12833
                     /locus_tag="OO330_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5883884.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12841..13434
                     /locus_tag="OO330_003107"
     CDS             12841..13434
                     /locus_tag="OO330_003107"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5883885.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13427..14563
                     /gene="hemW"
                     /locus_tag="OO330_003108"
     CDS             13427..14563
                     /gene="hemW"
                     /locus_tag="OO330_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5883886.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14877..15840
                     /locus_tag="OO330_003109"
                     /pseudo
     CDS             14877..15840
                     /locus_tag="OO330_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15885..16388
                     /locus_tag="OO330_003110"
     CDS             15885..16388
                     /locus_tag="OO330_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5883887.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGTQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4065 a         4218 c         4125 g         3984 t
ORIGIN      
        1 aaaagcagct agattatttt taagaatata taaaaagaaa gcgggccgat ttacgtaaat
       61 aaatcgaccc gccagcggaa taatcttaat tattcatctt caagataagt ataaccgtac
      121 aaacctgcct cgaactcttc aaggaactgt tgttgcagtt cagcatcaag atcggttttc
      181 ttcacttgat cgcggaactg ggttaacagc gttttcggat cgagctgtac atattgcagc
      241 atgtccgcca cggtatcgcc ttcgtcagac agttctactt ctacgctacc gtcagggaac
      301 acgaacacgt caactgcttc ggtatcaccg aacaggttgt gcatattgcc aaggatctcc
      361 tgatatgcac cgaccataaa gaaaccgagc atcggcggat tctctggatc gtactccggc
      421 attggcattg tcgtggcaat accatcacca tcaatatagt ggtcgatagc accgtcagag
      481 tcacaggtaa tatccagcag cacagcgcga cgttccggca cttgatccag cccttccagc
      541 ggtagaaccg ggaacaactg gtcgatcccc catgcgtccg gcattgactg gaacagcgag
      601 aaattgacgt acattttgtc cgccatacgt tcctgcagtt cgtcgattat cggacgatgc
      661 gcacggtttt gcggatccag ttgcttctgc acttcatggc acatgctcag atagagttgt
      721 tctgcccatg cacgttcttg caggctaaag gtgccggaag agtagccgat atgaatgtcg
      781 tgcagatcca tctggctgtc gtgtaaccat tcacgcaaag aacggcgagt tcccggttcg
      841 tgcatttcct gccaggtttc ccacatgctt tgcagcgcac gcggcgcatc ttctacgggc
      901 gcggtcggca ccgtatattc gttacgttcc acgccgatga tattcgacac cagcacggta
      961 tggtgcgcag tcaccgcacg gcccgattcg gtgattaccg tcggatgcgg cagaccattt
     1021 tcttcacacg catcgccaat cgcccagatg atgttgttgg cgtattcatt gaggccatag
     1081 tttacggagc agtcagactg cgaacgggtg ccttcataat ccacgcccag accgccgccg
     1141 acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat
     1201 tcacgaacgc ctgtcgcgat atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc
     1261 agtagttgca ggctgtcgag acgcccggct tcacgcaggg tttcaaccag ttgcagtacc
     1321 tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg aggactgcca tttacccgag
     1381 ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa cgacattcag acgttctgct
     1441 tcatccagca caatggcgat ttctgacatc ttctcaatga ccagatagac cttgtgcccc
     1501 atcttctcgc caattaatgc caggcggata tattcgcggt ctttataacc gttgcagacg
     1561 atgacgctac gagtcatgcc agcatgtgcc agcactgcca tcaactcggc tttggaaccg
     1621 gcttccagac ccagcggttc acccgaatga atcagggact cgatcacgcg gcggtgctgg
     1681 ttaactttga tcggataaac aaggaagtaa tcgccgttat agccgtagga ttccctcgca
     1741 cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac
     1801 agtgcaggca gacgctgacc ctgtgcttca cgagttttca ctaactgcgc gagatcgacg
     1861 cgagcttccg ggacgtccgg gtccgggcac acgctgatgt ggcccagctc gttaacgtca
     1921 tagtagttat tgccccacca ggcaatattg taagtacgca gcatcttgct ggcttcctgg
     1981 gagctcattg caacctcctg catggagcgt agtacaccgt gttcgcccgc tgacgaaggc
     2041 aaacccatag acatgtcgtc agacatagcg aacctcaaat tattttatta agtgtaaaac
     2101 agttaacgac tgtcgcagcc tggaactgcg ataacaaccc ataagcacat ggattttcca
     2161 gcagtgtatg ctgacgctcc gactgcgcaa ccggtttctt tttcataaca ttattaagca
     2221 cataaccgaa cgtaagtgtg acagttcggc gaaaccacga gaaaactctt gtttttacaa
     2281 gagcgccctt gttcagtcct cagtaactgt aaccagctct tgaatcctga gaagcgccga
     2341 gatgggtata acatcggcag gtatgcaaag cagagatgca gagtgcgggg aacgagtctt
     2401 caccagaacg gtgagacagg ttaagcagca gacaacggtt cattatttcg tatcacctcc
     2461 acggccgcct gttaagacga acccacaagc caaaactctg atttcaaccc ggctggaagt
     2521 ggcaacacga aagaaacgtc gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc
     2581 cacaatggca gaaaattgca aaagataaat acgcagaatg ccggcattgt caggaaaaat
     2641 ttccagccac gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa
     2701 gaattgacct aaaatagcca tccagatgtt aatccatcca taccgattaa cactcagact
     2761 gccagtgttt ttaacctgca gagtcgtggt aggatccgct accacagaaa atccacacaa
     2821 cagtttgagc taaccaaatt ctctttaggt gatattaaat atggcaaaac acctttttac
     2881 gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaatct ctgatgccgt
     2941 tttagacgcg atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa
     3001 aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga
     3061 gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc
     3121 taactcctgt gcggttctga gcgctatcgg caaacagtct cctgacatca accagggcgt
     3181 tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag ggtctgatgt ttggctacgc
     3241 aactaatgaa accgacgtgc tgatgccagc acctatcacc tatgcacacc gtctggtaca
     3301 gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag
     3361 ccaggtgact ttccagtatg acgacggcaa aatcgttggt attgatgctg tcgtgctttc
     3421 cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat
     3481 catcaagcca attctgcccg ctgaatggct gacttctgcc accaaattct tcatcaaccc
     3541 gaccggtcgt ttcgttatcg gtggcccaat gggtgactgc ggtctgactg gccgtaaaat
     3601 tatcgttgat acctacggcg gcatggcgcg tcacggtggc ggtgcattct ctggtaaaga
     3661 tccatcaaaa gtggaccgtt ccgcagccta cgcagcacgt tatgtcgcga aaaacatcgt
     3721 tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga
     3781 accgacctcc atcatggtag aaactttcgg tactgagaaa gtgccttctg aacaactgac
     3841 tctgctggta cgtgagttct tcgacctgcg cccatacgga ctgattcaga tgctggatct
     3901 gctgcacccg atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc
     3961 gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct gccggtctga agtaatcttt
     4021 cttcacctgc gttcaaaggc cagcctcgcg ctggcctttt tcttttacct caccgcggtg
     4081 gataggcgtt cacgccgcat ccggcaaaaa aactgcccgc acaataacat cattcttcct
     4141 gatcacgttt cacggcaaat taccatcaca actgaaaccg attacaccaa ccacaccagc
     4201 aaaatatttg taatattttc atattattat tcagctttca cagttgttac atttcttttc
     4261 agtaaagtct taattgcaga tgacagcgtt taatctatga tgatataact caattatttt
     4321 catgccctta aatcataact aagataaatg ttagtgtaag cgattacact gatgtgattt
     4381 gcttcacatc tttttacgtc gtactcacct atcttaattc acaataaaaa ataactatat
     4441 tggagggcat catgcctgac gctaaaaaac aggggcggtc aaacaaggca atgacgtttt
     4501 tcgtctgctt ccttgccgct ctggcgggat tactctttgg cctggatatc ggtgtaattg
     4561 ctggcgcact gccgtttatt gcagatgaat tccagataac ttcgcacacg caagaatggg
     4621 tcgtaagctc catgatgttc ggtgcggcag tcggtgcggt gggcatcggc tggctctcct
     4681 ttaaactcgg gcgcaaaaag agcctgatga tcggcgcaat cctgtttgtt gccggttcac
     4741 tgttctctgc ggctgcgcca aacgttgaag tactgattct ttcgcgcgtt ctgctggggc
     4801 tggcggtggg tgtggcctct tataccgcac cactgtacct gtcagaaatt gcgccggaaa
     4861 aaattcgcgg cagtatgatc tcgatgtatc agttgatgat cactatcggg atcctcggtg
     4921 cttatctttc tgataccgcc ttcagctaca ccggtgcatg gcgctggatg ctgggtgtga
     4981 ttatcatccc ggcaattttg ctgctgattg gtgtcttctt cctgccagac agcccacgtt
     5041 ggtttgccgc caaacgccgt tttgttgatg ccgaacgcgt gctgctacgc ctgcgtgaca
     5101 ccagcgcgga agcgaaacgc gaactggatg aaatccgtga aagtttgcag gttaaacaga
     5161 gtggctgggc gctgtttaaa gagaatagca acttccgccg cgcggtgttc cttggcgtac
     5221 tgttacaggt aatgcagcaa ttcaccggga tgaacgtcat catgtattac gcgccgaaaa
     5281 tcttcgaact ggcgggttat accaacacca ccgagcaaat gtgggggaca gtgattgtcg
     5341 gcctgaccaa cgtacttgcc acctttatcg caatcggcct tgttgaccgc tggggacgta
     5401 aaccaacgct aacgctgggc ttcctggtga tggctgctgg catgggcgta ctcggtacaa
     5461 tgatgcatat cggtatccac tctccgtcgg cgcagtattt cgccatcgcc atgctgctga
     5521 tgtttattgt cggttttgcc atgagtgccg gtccgctgat ttgggtactg tgctccgaaa
     5581 ttcagccgct gaaaggccgc gattttggca tcacctgctc caccgccacc aactggattg
     5641 ccaacatgat cgttggcgca acgttcctga ccatgctcaa cacgctgggt aacgccaaca
     5701 ccttctgggt gtatgcggct ctgaacgtac tgtttatcct gctgacattg tggctggtac
     5761 cggaaaccaa acacgtttcg ctggaacata ttgaacgtaa tctgatgaaa ggtcgtaaac
     5821 tgcgcgaaat cggcgctcac gattaatctc ctcaggcttc ctcccgtcgc ggaggaagcc
     5881 gcctcttgca gtcatctttt cttcgctcta tcctctgccg ctatgaaaac atcccgtctc
     5941 cctatcgcca tccaacaggc cgttatgcgc cgcctgcggg aaaaactcgc ccaggccaac
     6001 ctgaagctag ggcgtaacta cccggagcca aaactctctt acacccagcg cggaacctcc
     6061 gccggaacag cctggctgga aagctatgaa atccgcctca atcccgtttt gctgttggaa
     6121 aacagtgaag cttttattga agaagtggta ccgcacgaac tggcacattt gctggtatgg
     6181 aaacatttcg gccgcgtagc gccacatggc aaagagtgga agtggatgat ggaaagcgtg
     6241 ctgggcgttc ccgcccgtcg tacgcatcag ttcgaactgc aatccgtgcg tcgcaacacc
     6301 ttcccctacc gctgtaagtg ccaggaacat cagcttaccg tacgccgcca taatcgcgta
     6361 gttcgtggcg aggccatcta tcgctgtgtt cactgcggtg aacagctggt tgcgaaataa
     6421 ccacctgaac tatcaggaac tttcctgatc tggctgattg cataccaaaa caactttcgc
     6481 tacgttgctg gctcgtttta acacggagta agtgatgtac cgttatttgt ctattgctgc
     6541 ggtggtactg agcgcagcat tttccggccc gacgttggcc gaaggtatca atagtttttc
     6601 tcaggcgaaa gccgcggcgg taaaagtcca cgctgatgcg cccggtacgt tttattgcgg
     6661 atgtaaaatt aactggcagg gcaaaaaagg cgttgttgat ctgcaatcgt gcggctatca
     6721 ggtgcgcaaa aatgaaaacc gcgccagccg cgtagagtgg gaacacgtcg ttcccgcctg
     6781 gcaattcggt caccagcgcc agtgctggca ggacggtgga cgtaaaaact gcgctaaaga
     6841 tccggtctat cgcaagatgg aaagcgatat gcataacctg cagccgtcag tcggcgaggt
     6901 gaatggcgat cgcggcaatt ttatgtacag ccagtggaat ggcggcgaag gccagtacgg
     6961 tcaatgcgcc atgaaggtcg atttcaaaga aaaagctgcc gaaccaccag cgcgtgcacg
     7021 cggtgccatt gcgcgcacct acttctatat gcgcgaccaa tacaacctga cactctctcg
     7081 ccagcaaacg cagctgttca acgcatggaa caagatgtat ccggttaccg actgggagtg
     7141 cgagcgcgat gaacgcatcg cgaaggtgca gggcaatcat aacccgtatg tgcaacgcgc
     7201 ttgccaggcg cgaaagagct aacctacact agcgggattc tttttgttaa cccctacccc
     7261 acgcgtacaa ccgcgtgggg agacgacgcg gatttttaac tatgcgtatc ccccgcattt
     7321 atcatcctga accactgacc agccattctc acatcgcgct ttgcgaagat gccgccaacc
     7381 atatcgggcg cgtactgcgc atggggccgg ggcaggcgtt gcaattgttt gacggtagca
     7441 accaggtctt tgacgccgaa attaccagcg ccagcaaaaa aagcgtggaa gtgaaggtgc
     7501 tggaaggcca gatcgacgat cgcgaatctc cgctgcatat tcacctcggt caggtgatgt
     7561 cgcgtggtga aaaaatggaa tttactatcc agaaatcgat cgaactcggt gtaagcctca
     7621 ttacgccact tttttctgag cgctgcggcg ttaaactgga tagtgaacgt ctgaacaaga
     7681 agcttcagca gtggcagaag attgcaattg ctgcctgtga gcagtgtggt cgtaaccggg
     7741 tgccggaaat ccgtccagcg atggatctgg aagcctggtg tgcagagcag gatgaaggac
     7801 tgaaactgaa tcttcacccg cgcgccagta acagcatcaa tacgttgcct ttaccggttg
     7861 aacgcgtccg cctgctgatt ggcccggaag gcggtttatc ggcagatgaa attgccatga
     7921 ctgcccgcta tcaatttact gatatcctgt tgggacctcg cgttttgcgt acagagacaa
     7981 ctgcgctcac cgccattacc gcgctacaag tacgatttgg cgatttgggc taacggagaa
     8041 gaataatgat caagctcggc atcgtgatgg accccatcgc aaacatcaac atcaagaaag
     8101 attccagttt cgctatgttg ctggaagcac agcgtcgtgg ttacgaactt cactatatgg
     8161 agatgggcga tctgtatctg atcaatggcg aagcccgcgc ccatacccgc acgctgaacg
     8221 tgaagcagaa ctacgaagag tggttttcgt tcgtcggtga acaggatctg ccgctggccg
     8281 atctcgatgt gatcctgatg cgtaaagacc cgccgtttga taccgagttt atctacgcga
     8341 cctatattct ggaacgtgcc gaagagaaag ggacgctgat cgttaacaag ccgcagagcc
     8401 tgcgcgactg taacgagaaa ctgtttaccg cctggttctc tgacttaacg ccagaaacgc
     8461 tggttacgcg caataaagcg cagctaaaag cgttctggga gaaacacagc gacatcattc
     8521 ttaagccgct ggacggtatg ggcggcgcgt cgattttccg cgtgaaagaa ggcgatccaa
     8581 acctcggcgt gattgccgaa accctgactg agcatggcac ttgctactgc atggcgcaaa
     8641 attacctgcc agccattaaa gatggcgaca aacgcgtgct ggtggtggat ggcgagccgg
     8701 taccgtactg cctggcgcgt attccgcagg ggggcgaaac ccgtggcaat ctggctgccg
     8761 gtggtcgcgg tgaacctcgt ccgctgacgg aaagtgactg gaaaatcgcc cgtcagatcg
     8821 ggccgacgct gaaagaaaaa gggctgattt ttgttggtct ggatatcatc ggcgaccgtc
     8881 tgactgaaat taacgtcacc agcccaacct gtattcgtga gattgaagca gagtttccgg
     8941 tgtcgatcac cggaatgtta atggatgcca tcgaagcacg tttacagcag cagtaaccca
     9001 ccttagcgag aaggatctcg ttgagactct gagtgacagc gcccttcttt ccacgcatac
     9061 tgggcgctgt tgcttttttg aaccaggaaa cagaacctct gacaatgaat ttacagcatc
     9121 actttcttat tgccatgcct gctctccagg atccgatttt ccgtcgttcc gtggtctaca
     9181 tttgcgaaca taataccaat ggtgcaatgg ggatcatcgt caacaagccg ctggaaaatc
     9241 tcaaaattga agggattctg gaaaagctga agatcacgcc ggagccgcgt gatgaatcaa
     9301 tccgtctgga taaaccggtt atgctcggcg gcccactggc tgaagatcgc gggtttattt
     9361 tgcatactcc gccctccaat tttgcttcca gcattcgcat ttcagacaac acggtgatga
     9421 ccacttcccg cgatgtgctg gaaacgctcg gcaccgataa acaaccgtct gacgtattgg
     9481 tggctctggg ctatgcctcc tgggaaaaag gtcaactgga acaagaaatt ctcgataacg
     9541 cgtggctaac ggccccggcg gatctgaata ttctgttcaa aacgccgatt gccgaccgct
     9601 ggcgcgaggc ggcaaaactg attggtgtgg atattctcac catgcctggt gtggcaggac
     9661 acgcctgatg agtggaacct tactcgcctt cgacttcggc accaaaagca ttggcgtagc
     9721 ggtcggccaa cgcattaccg gcaccgctcg ccctttgcct gcaattaaag cgcaggacgg
     9781 tacgccggac tggaacctca tcgaacgttt actgaaagag tggcagcctg acgaaatcat
     9841 cgtcggtttg ccgctgaata tggacggcac cgaacagccg ttaaccgcca gagcgcgcaa
     9901 atttgccaac cgtattcatg gtcgtttcgg tgttgaagta aagctccatg acgagcgtct
     9961 tagcactgtg gaagcccgtt ccggtctttt tgaacagggc ggctatcggg cgctcaacaa
    10021 aggcaaagtt gactctgcct ctgcggttat tattctcgaa agctatttcg agcagggata
    10081 ttaaggcgat ctaaaagcct ggcggatagt cgttaccgcc atcttgccgg atgcggcgtg
    10141 aacgccttat ccagcacaca tctggcagcg gctgtaggtc tgataagacg cgttagcgtc
    10201 gcatcagaca tttattgcct ttataggccc tataaacttg cgcatcgggc atggcaacgt
    10261 cacaaacgcc cttcccccac ccgctgctga taactctgct gaaacgttaa catccccacc
    10321 tgctgcccgg tttgaataac atgcggtaac tggtgggttt tcccttcgcg aatcaaattc
    10381 cccaccgcgg gagtgttaat cagtaattca aacagcgcca cgcgtccttc ctgtttatcc
    10441 acttccagct tttgtgacag cactgcccgt aaactacccg caagttgatt acgtacggga
    10501 tctttttcct gcgccggaaa tgaatccacc agtcgctcaa ctgcctgcgc ggcaccacgt
    10561 gtatgtaagg ttgccagcac cagatgcccg gtttctgccg ccgtcagcgc cagacggatt
    10621 gtctcactgt cacgtagctc tccgagcaaa atcacatcag gatcttcccg caatgcagcc
    10681 cgcaaccccg atgcgaacgt catacagtgc aaaccaattt cccgctgctg gatcaaacat
    10741 cgctggctgg catagagata ttccacagga tcttccagcg tcagaatatg cgcatcggca
    10801 tgttgattaa gatagccaac catcgccgcc agcgtggtag atttgccact ccccgtagcc
    10861 cccgtcacca gaatcaggcc attctcgctc ttgagtaatt ccggcaatac cggtggtgca
    10921 ccaagctgtt cgagctgtgg acagtgcgaa ggtaacaacc gtaatgccag agaaatacct
    10981 tgccgttgcg cgaacgcact gccacgcaac cgctggtttt ccgccagcga cacggcaaaa
    11041 tccagctggc cattttccag caatattgcc cgctgatcgt catccagcca ctcccgcagt
    11101 agctcttcga cgtccggcgc atcaaacggc gcagcttcca ttctgccacg aatgcgccat
    11161 cgtgcgggcc aggcgctgca caggtgtaga tccgagacgt tatgctttac actaagggcc
    11221 acaatttctt ccatattcat actaagatcc tcggaaaatg aacgatattg cgcataacct
    11281 ggcacaggtc cgggacaaaa tctcagcggc tgcaacgcgt tgcggccgtt ctccagaaga
    11341 aattacgctg cttgcagtca gtaaaacaaa acctgcgagc gccatcgcag aagccattga
    11401 tgccgggcag cgtcaatttg gtgaaaacta cgttcaggaa ggggtagata aaattcgcca
    11461 ctttcaggaa ctgggcgtaa caggattaga atggcatttt attggcccgt tgcagtctaa
    11521 taaaagccgc ctagtggcag agcatttcga ctggtgtcat accatcgacc gtttgcgcat
    11581 tgctacccgc ctcaacgagc agcgcctggc agaacttccc cctcttaacg tactgattca
    11641 aattaatatt agtgatgaaa acagtaagtc cgggattcaa ctggcagaac tggacgagct
    11701 ggcagcggcg gtcgctgaac taccgcgttt acgtctgcgc gggctgatgg caatccctgc
    11761 gcctgagtca gaatatgtaa ggcagtttga agttgcacgc caaatggctg tagcatttgc
    11821 cggactgaaa acgcgctacc cgcatatcga cacgctctct ctgggaatgt cggacgatat
    11881 ggaagccgcc attgcggcag gtagcacgat ggttcgtatc ggcactgcaa tttttggtgc
    11941 gcgtgattac tctaaaaaat aaggaattaa aggaacgcca tgaatacgtt gactttcctg
    12001 ctttcaacgg tcattgagct gtatacaatg gtgctgttat tacgcatctg gatgcagtgg
    12061 gctcattgtg atttttacaa ccccttctca cagtttgtag tgaaggtagc gcagccaatt
    12121 atcgggccac tgcgccgcgt tattccggca atgggtccca ttgacagcgc ctcgctgctg
    12181 gttgcctata ttctcagttt tatcaaagcc attgtgctgt ttgaagtggt gaccttcctg
    12241 ccaatcatct ggattgccgg tttactgatt ctgctgaaaa ccatcggctt gctgattttc
    12301 tgggtcctgc tggtgatggc gattatgagc tgggtaagcc agggtcgtag cccgattgaa
    12361 tacgtgctga ttcagctggc cgatccgctg ctgcgcccga ttcgccgctt gctaccggca
    12421 atgggtggga ttgatttctc gccgatgatc ctcgttctgc tgctgtatgc catcaatatg
    12481 ggtgtcgcag aagtattaca ggcaaccgga aacatgctgc tgccggggct gtggatggcg
    12541 ttatgagtgc cgtaacagtt aatgatgacg gtctggtttt acggctctat attcagccga
    12601 aagccagccg tgattctatt gtcggtttac atggcgacga agttaaagtc gccattaccg
    12661 cgccgccggt tgacggccag gccaacagtc atctggtgaa gtttctcggt aagcaattcc
    12721 gggttgccaa aagccaggtg gtgattgaaa aaggcgaact tggccgccac aaacaaatta
    12781 aaatcattaa tccgcaacaa atcccgccag aaatcgcggc gttaattaat taggtatcct
    12841 atgcaaaaag ttgtcctcgc aaccggcaat gccggtaaag tacgtgagct ggcgtcgctg
    12901 cttagcgact ttggtcttga tatcgtggcc caaacagacc tcggcgttga ttccgctgaa
    12961 gaaaccggcc taacctttat cgaaaacgcg attctgaaag cgcgccatgc ggcaaaagtg
    13021 accggtttac cggcaattgc cgacgactct ggtctggcgg tagatgcgct gggtggcgcg
    13081 ccgggtattt actccgcgcg ttattccggt gaagacgcga ccgatcaaaa gaatctgcaa
    13141 aaactgttgg aaacactgaa agacgtaccg gacgaccaac gtcaggcgcg tttccactgc
    13201 gtgctggtat atctgcgtca cgcggaagat ccgactccgc tggtatgcca cggcagctgg
    13261 ccgggcgtga ttactcgcga accagcgggc actggtggct ttggttatga tccaatcttc
    13321 ttcgtacctt ccgaagggaa aaccgccgcc gaactgaccc gcgaagaaaa gagcgccatt
    13381 tcccaccgtg gtcaggcgtt gaaactgctg ctggacgctt tacgtaatgg ttaaattacc
    13441 gccgctgagt ctctacattc acatcccgtg gtgcgtgcag aaatgcccgt actgcgattt
    13501 caactctcac gcgttgaaag gagaagtgcc gcacgacgat tatgttcagc atctgctttg
    13561 cgatctggac aatgatgtgg cttacgctca gggccgtgaa gtaaagacaa tctttattgg
    13621 cggtggtacg ccgagcctgc tttccggccc ggcgatgcaa acgctgctag acggcgtgcg
    13681 tgcgcgtttg ccgctggcag cggatgcaga aattactatg gaagcgaacc caggcacggt
    13741 agaagcggat cgctttgtcg attatcagcg tgctggtgta aaccgcatct ctattggtgt
    13801 gcagagtttt agcgaagaaa agctgaaacg acttgggcgt attcatggcc cgcaagaagc
    13861 gaaacgagcg gcaaagctgg cgagcggttt agggttacgt agctttaacc ttgatttgat
    13921 gcatggacta ccggatcaat cactggaaga ggcgcttggc gatctacgcc aggccattga
    13981 actgaatccg ccgcatcttt cctggtatca actgactatc gaacctaata cgctgtttgg
    14041 ctcgcgaccg ccggtgctgc cggacgatga cgcgctgtgg gatattttcg aacaggggca
    14101 tcagttatta accgcagcgg gttatcagca atatgaaact tccgcttacg ccaaacccgg
    14161 ttatcagtgc cagcacaatc tcaactactg gcgctttggt gactacatcg gtattggctg
    14221 cggcgcgcac ggcaaagtga ccttcccgga tgggcgcatt ctgcgtacca ctaaaacgcg
    14281 tcatcctcgt ggttttatgc aaggaaggta tctggaaagc cagcgtgatg tcgaagccgc
    14341 agataagccg tttgagttct ttatgaatcg cttccgtctg ctggaggccg cgccgcgcgt
    14401 ggagtttatt gcgtataccg ggctttgcga agatgtgatt cgcccacagt tagacgaggc
    14461 gattgcccag ggttatctca ccgaatgtgc ggattactgg cagataacgg aacatggtaa
    14521 attattctta aactcacttc ttgaattatt cctcgctgag taatcattaa tgcgcctgcg
    14581 ggcgcattaa tcacttcttt tatccccccg ttaattaacc tcggcagaaa tatatttctt
    14641 actgaataaa tatcgacgac atttattata tatatttagt gaatatttac taatcattca
    14701 ttgattataa atatgtacaa cctggcgcac aaatttaata tttaatttct gactttattt
    14761 caagttgtga taaacataga caagttgtca gacagattaa ccaatttgtc gcacaaatca
    14821 ctgataagca caattagata tcaaccaatt tccatccttc aacagggagt ttttttatga
    14881 aaatgaggtc atttacccgc tcattagtct gcgcatcctt gctggctctg gttagcactg
    14941 gcgtaaacgc cgcagaaaaa gtggcgttaa agttagcaca caacctggaa agaagtcatg
    15001 ttgtacatca gagttttgaa gagctggcga aagaggtaaa acagctgtca aaaggtaata
    15061 tggtgatccg tatttatccc agcagccaga tgggtaatgc gcgagaaact atggaattgt
    15121 tgcaaaatgg cgcactggat atgaccaaag gttccgccag tgatctggag tcttttgata
    15181 atatttatgc tatctataat ttgccgttct tattaagttg tttttggtga agttggtaaa
    15241 gaaattatgg actcaacaaa agacaaaggc ttttttgctt tatcagctta tgtagcgggt
    15301 aatcgcagtt tctatgcgaa aaaaccgatt acaaagccag aagatcttaa aggactgaaa
    15361 attcgtgttc agccaagtcc gaccaccatc aaaatgattg aactgatggg tggttcaccc
    15421 actccaattt catttggcga agtttatact gcgatgcagc aaggtgtagt agatggcgca
    15481 gaaaataacg tgccttcctg gatgcagact cgacatattg aaattgccaa agttttttct
    15541 gaagatgaac acgcctcaat tcctgacttt ttggttatct ccaccaaaac atggaataaa
    15601 ttaaccccag aacagcaaca aatcctcgaa acagcggcga agaaatccga agcttatcaa
    15661 caaaaattgt gggaaaaaat tgacgctgac acacgtgctc aggcgaaagc tatggggggt
    15721 gaaattgtaa aagtggataa agcgccattc cgtaaagccg tgcaacctct gtttgacgac
    15781 ttcaaaaaag atccaaaaca agctgctttg ttagagaaat tcgacaacgc tgctcaataa
    15841 tattccgggc cagcctctgg ccctgatttt ccaggatgct tacaatgata tttaaccgcc
    15901 tgaaacttgc ggtagaccgc gtcattgccg cgttttctgt cgccgtaatg cttgctctgg
    15961 ttgtatgcgt agtctggcaa gtatttagcc gctacgtact taaccagcca agtacattaa
    16021 ccgatgagct ggcacgcttt ttgatgatct gggtaggatt attaggtgct gcatataccg
    16081 tcggcacgca gcgacatctc tctattgatt tatttgctct cgccctgaat aagcgcaaac
    16141 aattacttct tagtatagtc attaacgttc tcattttagg ttttgccggt tcggtcattg
    16201 tgactggtgg cctgaaatta attgataaaa cactggcaac atcacaggta tctgccgcta
    16261 tgcaaatacc gatgggatac gtatatatca ttctgccatt aagtggagtg gtaatgatgt
    16321 tttatgcctt gtgttttatt aatcaaagca ttcaacaact gaaacaacct gcgcaggagg
    16381 ccagttaatg ga
//
LOCUS       DAJTRB010000087        16192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTRB010000087 DAJTRB010000000
VERSION     DAJTRB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.087"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..73)
                     /locus_tag="OO330_003111"
     CDS             complement(<1..73)
                     /locus_tag="OO330_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5883888.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO330_003112"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO330_003112"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5883889.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO330_003113"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO330_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5883890.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO330_003114"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO330_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5883891.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO330_003115"
     CDS             2703..2939
                     /locus_tag="OO330_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5883892.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO330_003116"
     CDS             2942..3799
                     /locus_tag="OO330_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5883893.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="OO330_003117"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="OO330_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO330_003118"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO330_003118"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5883894.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="OO330_003119"
     CDS             complement(6414..7310)
                     /locus_tag="OO330_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5883895.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="OO330_003120"
     CDS             complement(7359..7598)
                     /locus_tag="OO330_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5883896.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="OO330_003121"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="OO330_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5883897.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9435
                     /gene="nemA"
                     /locus_tag="OO330_003122"
                     /pseudo
     CDS             8337..9435
                     /gene="nemA"
                     /locus_tag="OO330_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="OO330_003123"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="OO330_003123"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5883898.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="OO330_003124"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="OO330_003124"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5883899.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="OO330_003125"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="OO330_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..>16192)
                     /locus_tag="OO330_003126"
     CDS             complement(15268..>16192)
                     /locus_tag="OO330_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5883900.1"
                     /translation="KRAAFPRFKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRN
                     SRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTV
                     FEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTT
                     TVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEY
                     KQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNIL
                     AAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3688 a         4287 c         4250 g         3967 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccggctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gcaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccgact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccgggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctgggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccgcaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acagcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacggcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgtttgaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatggc gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctggcctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tc
//
LOCUS       DAJTRB010000088        15685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTRB010000088 DAJTRB010000000
VERSION     DAJTRB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.088"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..175
                     /gene="tuf"
                     /locus_tag="OO330_003127"
     CDS             <1..175
                     /gene="tuf"
                     /locus_tag="OO330_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5883901.1"
                     /translation="VTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGG
                     RTVGAGVVAKVLG"
     gene            358..552
                     /gene="bfd"
                     /locus_tag="OO330_003128"
     CDS             358..552
                     /gene="bfd"
                     /locus_tag="OO330_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5883902.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            625..1101
                     /gene="bfr"
                     /locus_tag="OO330_003129"
     CDS             625..1101
                     /gene="bfr"
                     /locus_tag="OO330_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5883903.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1098..1565)
                     /locus_tag="OO330_003130"
     CDS             complement(1098..1565)
                     /locus_tag="OO330_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5883904.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1946..2257
                     /gene="rpsJ"
                     /locus_tag="OO330_003131"
     CDS             1946..2257
                     /gene="rpsJ"
                     /locus_tag="OO330_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5883905.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2290..2919
                     /gene="rplC"
                     /locus_tag="OO330_003132"
     CDS             2290..2919
                     /gene="rplC"
                     /locus_tag="OO330_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5883906.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2930..3535
                     /gene="rplD"
                     /locus_tag="OO330_003133"
     CDS             2930..3535
                     /gene="rplD"
                     /locus_tag="OO330_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5883907.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3532..3834
                     /gene="rplW"
                     /locus_tag="OO330_003134"
     CDS             3532..3834
                     /gene="rplW"
                     /locus_tag="OO330_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5883908.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3852..4673
                     /gene="rplB"
                     /locus_tag="OO330_003135"
     CDS             3852..4673
                     /gene="rplB"
                     /locus_tag="OO330_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5883909.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4690..4968
                     /gene="rpsS"
                     /locus_tag="OO330_003136"
     CDS             4690..4968
                     /gene="rpsS"
                     /locus_tag="OO330_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5883910.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4983..5315
                     /gene="rplV"
                     /locus_tag="OO330_003137"
     CDS             4983..5315
                     /gene="rplV"
                     /locus_tag="OO330_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5883911.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5333..6034
                     /gene="rpsC"
                     /locus_tag="OO330_003138"
     CDS             5333..6034
                     /gene="rpsC"
                     /locus_tag="OO330_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5883912.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6047..6457
                     /gene="rplP"
                     /locus_tag="OO330_003139"
     CDS             6047..6457
                     /gene="rplP"
                     /locus_tag="OO330_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5883913.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6457..6648
                     /gene="rpmC"
                     /locus_tag="OO330_003140"
     CDS             6457..6648
                     /gene="rpmC"
                     /locus_tag="OO330_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5883914.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6648..6902
                     /gene="rpsQ"
                     /locus_tag="OO330_003141"
     CDS             6648..6902
                     /gene="rpsQ"
                     /locus_tag="OO330_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5883915.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7067..7438
                     /gene="rplN"
                     /locus_tag="OO330_003142"
     CDS             7067..7438
                     /gene="rplN"
                     /locus_tag="OO330_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5883916.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7449..7763
                     /gene="rplX"
                     /locus_tag="OO330_003143"
     CDS             7449..7763
                     /gene="rplX"
                     /locus_tag="OO330_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5883917.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7778..8317
                     /gene="rplE"
                     /locus_tag="OO330_003144"
     CDS             7778..8317
                     /gene="rplE"
                     /locus_tag="OO330_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5883918.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8332..8637
                     /gene="rpsN"
                     /locus_tag="OO330_003145"
     CDS             8332..8637
                     /gene="rpsN"
                     /locus_tag="OO330_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5883919.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8671..9063
                     /gene="rpsH"
                     /locus_tag="OO330_003146"
     CDS             8671..9063
                     /gene="rpsH"
                     /locus_tag="OO330_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5883920.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9076..9609
                     /gene="rplF"
                     /locus_tag="OO330_003147"
     CDS             9076..9609
                     /gene="rplF"
                     /locus_tag="OO330_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5883921.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9619..9972
                     /gene="rplR"
                     /locus_tag="OO330_003148"
     CDS             9619..9972
                     /gene="rplR"
                     /locus_tag="OO330_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5883922.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9987..10490
                     /gene="rpsE"
                     /locus_tag="OO330_003149"
     CDS             9987..10490
                     /gene="rpsE"
                     /locus_tag="OO330_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5883923.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10494..10673
                     /gene="rpmD"
                     /locus_tag="OO330_003150"
     CDS             10494..10673
                     /gene="rpmD"
                     /locus_tag="OO330_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5883924.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10677..11111
                     /gene="rplO"
                     /locus_tag="OO330_003151"
     CDS             10677..11111
                     /gene="rplO"
                     /locus_tag="OO330_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5883925.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11119..12450
                     /gene="secY"
                     /locus_tag="OO330_003152"
     CDS             11119..12450
                     /gene="secY"
                     /locus_tag="OO330_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5883926.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12482..12598
                     /gene="rpmJ"
                     /locus_tag="OO330_003153"
     CDS             12482..12598
                     /gene="rpmJ"
                     /locus_tag="OO330_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5883927.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12745..13101
                     /gene="rpsM"
                     /locus_tag="OO330_003154"
     CDS             12745..13101
                     /gene="rpsM"
                     /locus_tag="OO330_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5883928.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13118..13507
                     /gene="rpsK"
                     /locus_tag="OO330_003155"
     CDS             13118..13507
                     /gene="rpsK"
                     /locus_tag="OO330_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5883929.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13541..14161
                     /gene="rpsD"
                     /locus_tag="OO330_003156"
     CDS             13541..14161
                     /gene="rpsD"
                     /locus_tag="OO330_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5883930.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14187..15176
                     /gene="rpoA"
                     /locus_tag="OO330_003157"
     CDS             14187..15176
                     /gene="rpoA"
                     /locus_tag="OO330_003157"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5883931.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15217..15600
                     /gene="rplQ"
                     /locus_tag="OO330_003158"
     CDS             15217..15600
                     /gene="rplQ"
                     /locus_tag="OO330_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5883932.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4039 a         3684 c         4181 g         3781 t
ORIGIN      
        1 cgtgactggt accatcgaac tgccggaagg cgtagagatg gtaatgccgg gcgacaacat
       61 caaaatggtt gttaccctga tccacccgat cgcgatggac gacggtctgc gtttcgcaat
      121 ccgtgaaggc ggccgtaccg ttggcgcggg cgttgttgct aaagttctgg gctaattaca
      181 cgttaattag ttttgaattg aaaagggcgc ttcggcgcct tttttgctgc ccgtaatttg
      241 agctcgaaat atttagtcgt aattttgctc gctgaactat ggcgcaaata aaactgtgct
      301 attgtaatca taaccattct catttacact ttgtgcagaa tttgaacggg agcaatcatg
      361 tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc gccaggcagt ccgtcagttt
      421 catccgcaat ctttccaaca attgcgtaag tttattcctg tgggaaatca atgcggtaag
      481 tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga tgcagatgcc agaatttaaa
      541 gagattgcct gaagcccacg cttatttttt gacatcctgc tagccccatc tacgctctaa
      601 tagtggaagc ggagggacta taaaatgaaa ggtgatgtta aaatcataaa ttatctcaat
      661 aaactattgg gaaatgagct tgtcgcaatc aatcagtatt ttctccatgc ccggatgttt
      721 aaaaactggg gcctgatgcg tctcaatgac gtcgaatacc atgaatccat cgatgagatg
      781 aaacacgctg ataaatatat cgagcgtatt ttgtttttag aaggaatccc caacttacag
      841 gatttaggca aactggggat tggtgaagat gtcgaggaga tgctgcaatc tgacctgcga
      901 ttagaactgg aaggcgcgaa ggatctgcgt gaggctatcg catatgccga cagcgttcat
      961 gactatgtta gccgcgatat gatgatcgag atccttgctg atgaagaagg tcatatcgac
     1021 tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc tacaaaatta cctgcaatca
     1081 caaattaaag ttaaagatta acggcctatc agcaaactat cccagcctat aacgaaaccc
     1141 gcagccgcca gaaatggccc aaaaggcagc gggtttttta atgattcttt tcctctcatg
     1201 agcagaccaa taactacggc tccgcaagcg aatgaggcag caaggaaaac cagtcgtggt
     1261 aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa acttcacatc accatagcct
     1321 aatccttctt tatgtcgcag tatgcgatac ccccagtaaa tgacagcgaa tgtgccgtaa
     1381 ccaacaattg ccccccataa cgcatcggct aaaccatcag gatgacaaac ttgatagaac
     1441 aaaagacctg accagagtaa cgggcacgta aatctgtcag gtaacaagcc atgctttgca
     1501 tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa ttagaaaggg aagggtagtg
     1561 tccataaaac atcctctgta attggagtat caccatcgaa taactccgga tgctcagcaa
     1621 cagagagaga gcgctgattt gttaacctga ttccggtgaa aaacgctatc ttcacaacct
     1681 tacaaatttc gtggaatgca gctcgcaatc tgcgcgttgc gattaatttc tacccgatgc
     1741 ttgcgtcagg ctcagatttg cgtataatgc gcgggcttgt cgtagttgac agcaggttca
     1801 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
     1861 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
     1921 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
     1981 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
     2041 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
     2101 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
     2161 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
     2221 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
     2281 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
     2341 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
     2401 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     2461 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     2521 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     2581 gttgaactgt ttgctgacgt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     2641 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     2701 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     2761 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     2821 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     2881 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     2941 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     3001 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     3061 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     3121 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     3181 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     3241 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     3301 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     3361 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     3421 gcagaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     3481 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     3541 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     3601 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     3661 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     3721 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     3781 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     3841 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     3901 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     3961 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     4021 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     4081 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     4141 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     4201 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     4261 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     4321 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     4381 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     4441 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     4501 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     4561 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     4621 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     4681 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     4741 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     4801 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     4861 gttccggtat ttgtaaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     4921 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     4981 agatggaaac tattgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     5041 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     5101 acaagaaagc ggctgtacag gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     5161 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     5221 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     5281 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     5341 gaaagtacat cctaatggta ttcgcttggg tattgtaaaa ccatggaact ctacttggtt
     5401 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     5461 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     5521 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     5581 cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat
     5641 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     5701 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     5761 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     5821 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     5881 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     5941 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     6001 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     6061 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     6121 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     6181 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     6241 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     6301 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     6361 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     6421 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     6481 aagagcattg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     6541 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     6601 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     6661 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     6721 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     6781 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     6841 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     6901 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     6961 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     7021 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     7081 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     7141 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     7201 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     7261 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     7321 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     7381 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     7441 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     7501 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     7561 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     7621 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     7681 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     7741 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     7801 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     7861 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     7921 actgctggat aacgcagcag cagacctagc agcaatctcc ggtcaaaaac cgctgatcac
     7981 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     8041 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     8101 acctcgtatc cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     8161 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     8221 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     8281 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     8341 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     8401 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     8461 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     8521 tgccgtcaaa caggtcgtcc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     8581 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     8641 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     8701 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     8761 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     8821 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     8881 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     8941 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     9001 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     9061 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     9121 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     9181 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     9241 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     9301 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     9361 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     9421 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     9481 gctgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     9541 ccttacaaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     9601 aagaagtaag gtaacactat ggataagaaa tctgctcgta tccgtcgtgc gacccgcgca
     9661 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     9721 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     9781 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     9841 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     9901 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     9961 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
    10021 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
    10081 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
    10141 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
    10201 ttaacgtcgc gctgaataac ggcactctac aacaccctgt taaaggtgtt cacacgggtt
    10261 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
    10321 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
    10381 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
    10441 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
    10501 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
    10561 cgctgcttgg cttgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
    10621 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
    10681 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
    10741 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
    10801 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
    10861 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctatctgacc
    10921 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
    10981 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
    11041 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
    11101 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
    11161 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
    11221 attggctctt ttattccgat ccctggtatt gatgccgctg tacttgccaa actgcttgag
    11281 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
    11341 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
    11401 ctgacggtgg ttcacccaac gttggcagaa attaagaaag aaggggagtc tggtcgtcgt
    11461 aagatcagcc agtacacccg ttacggtact ctggtgctgg caatattcca gtcgatcggt
    11521 attgctaccg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    11581 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    11641 ggcgaacaga tcactgaacg aggtatcggc aacggtattt caatcattat cttcgccggt
    11701 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    11761 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    11821 gtgtttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    11881 cgtcgtgtct atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    11941 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    12001 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    12061 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    12121 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    12181 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    12241 ttggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    12301 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    12361 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    12421 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    12481 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    12541 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    12601 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    12661 tgtatgtctg tacgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    12721 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    12781 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    12841 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    12901 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    12961 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    13021 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    13081 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    13141 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    13201 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    13261 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    13321 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    13381 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    13441 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    13501 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    13561 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    13621 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    13681 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    13741 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    13801 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    13861 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    13921 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    13981 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    14041 gccaacttgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    14101 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    14161 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    14221 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    14281 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    14341 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    14401 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    14461 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    14521 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    14581 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    14641 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    14701 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    14761 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    14821 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    14881 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    14941 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    15001 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    15061 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgttggct tcccgtggac
    15121 tgtctctggg catgcgcttg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    15181 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    15241 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    15301 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    15361 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    15421 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    15481 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    15541 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    15601 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    15661 ttatctacaa tagctgtact ctttt
//
LOCUS       DAJTRB010000089        15411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTRB010000089 DAJTRB010000000
VERSION     DAJTRB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.089"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..1176)
                     /locus_tag="OO330_003159"
     CDS             complement(<1..1176)
                     /locus_tag="OO330_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5883933.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1375..1459)
                     /locus_tag="OO330_003160"
     tRNA            complement(1375..1459)
                     /locus_tag="OO330_003160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Leu,seq:caa)
     gene            1655..2674
                     /gene="ahr"
                     /locus_tag="OO330_003161"
     CDS             1655..2674
                     /gene="ahr"
                     /locus_tag="OO330_003161"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5883934.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2803..4305
                     /locus_tag="OO330_003162"
     CDS             2803..4305
                     /locus_tag="OO330_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5883935.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OO330_003163"
     CDS             complement(4424..5506)
                     /gene="lptG"
                     /locus_tag="OO330_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5883936.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OO330_003164"
     CDS             complement(5506..6606)
                     /gene="lptF"
                     /locus_tag="OO330_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5883937.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6873..8384
                     /gene="pepA"
                     /locus_tag="OO330_003165"
     CDS             6873..8384
                     /gene="pepA"
                     /locus_tag="OO330_003165"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5883938.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8518..8961
                     /gene="holC"
                     /locus_tag="OO330_003166"
     CDS             8518..8961
                     /gene="holC"
                     /locus_tag="OO330_003166"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5883939.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8961..11816
                     /gene="valS"
                     /locus_tag="OO330_003167"
     CDS             8961..11816
                     /gene="valS"
                     /locus_tag="OO330_003167"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5883940.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLFGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11870..13066)
                     /locus_tag="OO330_003168"
     CDS             complement(11870..13066)
                     /locus_tag="OO330_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5883941.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13259..13762
                     /locus_tag="OO330_003169"
     CDS             13259..13762
                     /locus_tag="OO330_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5883942.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13808..14224)
                     /gene="rraB"
                     /locus_tag="OO330_003170"
     CDS             complement(13808..14224)
                     /gene="rraB"
                     /locus_tag="OO330_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5883943.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14386..15399
                     /gene="argF"
                     /locus_tag="OO330_003171"
     CDS             14386..15399
                     /gene="argF"
                     /locus_tag="OO330_003171"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5883944.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3975 a         3934 c         3965 g         3537 t
ORIGIN      
        1 catcaaccta cgctccccaa gatgttccgc cttatggatg taagctgctc gcacagagct
       61 acgctcctgg tgactcatct gccgctctac cgcatccctc gaccataatc ccgactcaat
      121 caacgaacta caagccatcg tcctgaagcc atgcccacaa acctcgacct tcgtgtcata
      181 ccccattacg cgcaaagcct tgttcaccgt attctcactc atcggcttac gtggatcgtg
      241 atcgcccaca aaaatcagct ctcgatttcc gctcatgctt ttgatctttt ccaaaatact
      301 tagggcctgc cgcgacaaag gtacaaggtg tggtgtccgc atcttcgagc cacgctgaga
      361 atgcttgact ccttccagtt gctcacgctc acctgggatt gtccacatag ccgtttcaaa
      421 atctatttct gaccagcggg caaaacgcag ctcgctcgaa cggatgaaaa ccaataaagt
      481 gagttcaacc gccagtcgag ttaacggtct gccagaatag tgatctatgc gatgaagtaa
      541 ttcaggtata cggttcagtt caagagctgc acggtgctgt cttttcgccg tagcaaccgc
      601 acctgcaatc tcttgcgcgg ggttgtagtc gattaagccg ctctgaacgg caaagcgcat
      661 aatcgcggta gtacgctgct gtaaacgggc ggcaacttcg agccgcccgg atgactcgac
      721 tgctttaatg ggtacaagaa gatcccgcgt cttcagttcc gcaatgttcc gcttaccgat
      781 agcagtaaag agattatcct ccagactttt caaaacacga gcgctatgcg atgccgacca
      841 cttctgattg ctggcgtgcc agtctctggc gactacttca aacgttatcg cctcttgttc
      901 ctgttctacc ttaacagctt tcttgttttc actgggatcg acaccattag ctaaaagctt
      961 acgagcctca tcccggcgtg ccctggcatc cgccaacgac acttcggggt atttccccaa
     1021 tgccagcatc ttctctttgc cgccaaagcg ataacgtagc cgccagtatt tggagccgtt
     1081 agggtgaacc agcaaaacca tgccttcacc gtcggtcagc ttataggctt ttgcttcagg
     1141 cttagccgaa cgaaccttca catcactcag agccatgatg agtatccttt caagggttct
     1201 gtgtgggtac aaacattatc gaaccgggaa atacccgcag ttgtacccgc atcagtaagt
     1261 tgatgtagat tgaatcaggt tgacttaggt tgagtgaaaa agcgaggaaa gccttgcgga
     1321 tactggattt taggcacaaa aaaagacgtc cgttgacgtc tattgatgtt ccgatggtgc
     1381 cgaaggccgg actcgaaccg gcacgtattt ctacggttga ttttgaatca actgcgtcta
     1441 ccgatttcgc cacttcggca ctgaagaggt atgcggaaaa cgttgtggat tatacctgtc
     1501 gcacgccacc atgcaagcgc cagcttgcac ccacctcgct aagtgctgaa attttcagca
     1561 ttacactcag cgatatgtaa cctttgtcac actccaggca ccccgccctg ccatgctcta
     1621 cacttcccaa acaacaccag agaaggcccc aaaaatgtcg atgataaaaa gctatgccgc
     1681 aaaagaagcg ggcggcgaac tggaagttta tgagtacgat cccggtgagc tgaagccaca
     1741 agatgttgaa gtgcaggtgg attactgcgg gatttgtcat tccgatctgt cgatgatcga
     1801 taacgaatgg ggattttcac aatatccgct ggttgccggg catgaggtga ttgggcgcgt
     1861 agtggcactc gggagcgccg cgcaggataa aggtttgcag gtcggtcagc gtgtcgggat
     1921 tggctggacg gcgcgtagct gtggtcactg cgacgcctgt attagcggta atcagattaa
     1981 ctgcgagcaa ggtgcggtgc cgacaattat gaatcgcggt ggctttgccg agaagttgcg
     2041 tgcggactgg caatgggtga ttccactgcc agaaaatatt gatatcgagt ccgccgggcc
     2101 gctgttgtgc ggcggtatca cggtctttaa accactgttg atgcaccata tcactgctac
     2161 cagccgcgtt ggggtaattg gtattggcgg gctggggcat atcgctataa aacttctgca
     2221 cgcaatggga tgcgaggtga cggcctttag ttctaatccg gcgaaagagc aggaagtact
     2281 ggcgatgggt gccgataaag tggtgaatag ccgcgatccg caggcactga aagccctgtc
     2341 ggggcagttt gatctcatta tcaatactgt gaacgtcagc ctcgactggc agccttattt
     2401 tgaggcgctg acctatggcg gtaatttcca tacggtcggt gcggttctca cgccgctgtc
     2461 tgttccggcc tttacgttaa ttgcgggcga ccgcagcatc tctggttctg ctaccggcac
     2521 gccttatgag ctgcgaaagc tgatgcgctt tgccgcccgc agcaaggttg cgccgacaac
     2581 cgaactgttc ccgatgtcga aaattaacga cgccattcag catgtacgcg atggtaaagc
     2641 gcgctaccga gtagtcctga aagccgactt ctgatccctt tgccgggtgc tgtcgcccgg
     2701 cacttaaatc tctttggaag gcgtctcgca atctgctgaa tttagcggtg aagagtgcac
     2761 agccgttgcc tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta
     2821 attgcccgca cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg
     2881 ctgattactg gcgcaacggg gacgggtaaa accgttacgc tgcaaaaact ggcagagtca
     2941 ttgtcggaaa tcggcgtgcc ggtgtttatg gctgatgtga aaggcgatct gaccggtatc
     3001 gcgcaggcag gaacggcgtc ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat
     3061 gactggcaac cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccac
     3121 acggtgcggg cgacggtttc ggatctgggg ccgctgttgc tggcgcgact gttgaatctc
     3181 aacgatgtgc aatctggcgt gctgaatatt atcttccgca ttgcggacga tcagggattg
     3241 ttgctgctcg actttaaaga tttgcgggcg attacccagt acatcggcga taacgccaaa
     3301 tccttccaga atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcgga
     3361 ttactgtcgc tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc
     3421 aaagactgga tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag
     3481 aaactttatc agatgccgaa actgtacgcc gccagcctgc tgtggatgct ttcagagttg
     3541 tatgaacaat tgccggaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac
     3601 gaggcacatc tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg
     3661 ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat
     3721 attccggata atgtgttcgt ccagctcggt aatcgtgttc agcacgcttt gcgggctttt
     3781 acgccaaaag atcagaaagc agtgaagaca gcggcgcaaa ccatgcgggc caatccggca
     3841 tttgataccg aaaaggcaat ccaggaactg gggaccggcg aggcgttaat ctcgtttctc
     3901 gatgcaaaag gaagtccttc tgtggtggaa cgggcgatgg tgatcgcacc ttgttcgcga
     3961 atggggccgg tgacggaaga tgagcgtaat ggcctgatta atcactcccc ggtgtatggc
     4021 aaatatgaag atgaggtgga ccgcgagtcc gcctatgaga tgctgcaaaa aggctttcag
     4081 gccagtaccg agcagcaaaa taatcccccc gtgaaaggga aagaggtggc ggtggatgac
     4141 ggtattcttg gtggattgaa ggatattttg tttggcacta ccggaccacg cggcgggaag
     4201 aaagatggtg tggtgcaaac aatggcgaaa agcgccgctc gccaggtgac gaatcagatt
     4261 gtacgcggga tgttggggag tttgctgggg gggagaaaaa gataagtcag acgtgatctg
     4321 tgcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcgaac tcgtatttga
     4381 ctttgtcaac aataataaag ggagctttcg ctcccttgtt agattacgat tttctcatta
     4441 acagccacag gctgattaag aagaagctgg cacttggcaa cagtgcgccg atgatcggcg
     4501 ggatgccgta aaccaacgtc agcgggccga agatctggtc cagtacgtag aagacaaaac
     4561 cgaagctgat acctgtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga
     4621 taaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag attttgctcc
     4681 acatgttgag ctgataacgt ccggcatcct gaccgctcga cttcagatac ttcacgtagt
     4741 tgtgcaaacc actgatggag agtgcatccg ggtccagcgc caccacgccc agtttgtctg
     4801 gcgtgaggtc ggttttccag gtgccgctca ccgtctgcga accggtaatc tgtttcggat
     4861 tggtcagatc agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact
     4921 tcgcggtagc ggcataacgt acggattgca gacgacgatt ctcgttaaag gcataaatac
     4981 tgatgccacc taacacctcg tcacctttaa cccgctcaat gtagacgaag ttgttgccat
     5041 ctttcgccca taaaccttgc tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac
     5101 ggtagttacg cgccatctgt tcgccctgcg gcgcgaccca ttcgccaatc gccatcgtca
     5161 gcaagaccag tggaatggcg gttttcatca ccgacagcgc tacctgcata cgggtaaaac
     5221 cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg
     5281 ccccaagaag agccgccatc gggaagaaga tctgcacatc tttcggcacg ctcagcaagg
     5341 tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc agctgatcga
     5401 caaatttgat aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga
     5461 aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt
     5521 acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat
     5581 cgccaaagcc agataaatca ggttaacggt ccacatccac agtgtcgggt ccagcttacc
     5641 tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat acagcagcat
     5701 ggctggcagc atcgacagta cgcgtccctg acgcgggtta accacgctca acggtacaac
     5761 cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca gttctgcgcg
     5821 agcacgatcg gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg tatcgttcgg
     5881 gtcgagcgcc accgcctggt gaccaatgat cgcctgataa tcctggaaat ccgtaatgcg
     5941 gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg
     6001 ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc
     6061 attacctttt ggtcgaattt gtgcgaggaa cacatctttg aaatcgctgc cgtcaacgct
     6121 ttcgatgaac agcaccgagc tgccattagt cgcttgctgg aattgccctt gcgccagcgc
     6181 cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca
     6241 cggtcccgcc cacatcacgt taaccgccgc gacgattgcc gtgaataccg caaggatcat
     6301 tgccgctttc accagaaccg ctttgctcaa gccgcaggca tgcattaccg taatttcact
     6361 ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat
     6421 aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat
     6481 atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag
     6541 caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat
     6601 tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg
     6661 cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac
     6721 ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca
     6781 cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc
     6841 gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc
     6901 cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc
     6961 cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgccggg
     7021 gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgttccg aatgtacttt
     7081 ccgagcgaat tctccttatt ggttgcggca aagaacgtga gctggatgag cgtcagtaca
     7141 agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcgatg gaagcggtct
     7201 gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg
     7261 tcgagacagc aaaagagacg ctctacagtt tcgatcagtt gaaaacgaat aagagcgaac
     7321 cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg
     7381 gtgagcgcgc gatccagcac ggtctggcga ttgccgccgg gataaaagca gcgaaagatc
     7441 tcggtaatat gccgccgaat atctgtaacg ccgcttatct cgcctctcag gcgcgccagc
     7501 tggctgacag ctacagcaag aatgtcatca cccgcgttat cggcgaacag cagatgaaag
     7561 agctgggaat gcactcttat ctggcggtcg gtcagggttc acaaaacgaa tcgctgatgt
     7621 cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta
     7681 aaggtttaac cttcgactcc ggcggtatct ccatcaagcc ttcagaaggc atggatgaga
     7741 tgaagtacga tatgtgcggc gcggcggcag tttacggcgt gatgcgtatg gtcgcggagc
     7801 tgcaactgcc gattaacgtt attggcgtgc tggcaggctg tgaaaacatg cctggcgggc
     7861 gtgcctatcg tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga
     7921 acaccgatgc cgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gaacgttttg
     7981 agccggaagc agtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc
     8041 atcatattac tggtctgatg gcgaaccata atccgctggc ccatgaactg attgccgcgt
     8101 ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacagc
     8161 tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg
     8221 caggttgctt cctgtcacgc tttacccgta agtataactg ggcgcacctg gatattgcag
     8281 gaaccgcctg gcgttctggt aaagcaaaag gcgcaaccgg tcgtccggta gcgttgctgg
     8341 cacagttcct gttaaaccgc gctgggttta acggcgaaga gtaagtttgc gttttaccct
     8401 caccctaacc ctctccctaa gggagaggga accgatcgag cgcaatagaa attcattgct
     8461 catctttcag ggtgaggaat gcagcaatat ttaaatccac cacaagaagc cccatatatg
     8521 aaaaacgcaa cgttctacct tctggacaat gacaccaccg tcgatggctt aagcgccgtt
     8581 gagcaactgg tgtgtgaaat tgccgcagaa cgttggcgca gcggtaagcg cgtactcatc
     8641 gcctgtgaag atgaaaagca ggcttaccgg ctggatgaag ccctgtgggc gcgtccggca
     8701 gaaagctttg ttccgcataa tttagcggga gaaggaccgc gcggcagtgc gccggtggag
     8761 atcgcctggc cgcaaaaacg cagcagcagc ccgcgcgata tattgattag tctgcgaaca
     8821 agctttgcag attttgccac cgctttcaca gaagtggtag acttcgttcc ttatgaagat
     8881 tctctgaaac aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg
     8941 aatacggcaa cctggaaata atggaaaaga catacaaccc acaagatatc gaacagccgc
     9001 tttacgagca ctgggaaaag cagggctact ttaagcctaa tggcgatgaa agccaggaaa
     9061 gtttctgcat catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct
     9121 tccagcaaac catcatggat accatgatcc gctatcagcg catgcagggc aaaaacaccc
     9181 tgtggcaggt cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga
     9241 ttgccgcaga agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa
     9301 tctgggaatg gaaagcggaa tctggcggca ccattacccg tcagatgcgc cgtctcggca
     9361 actccgtcga ctgggagcgt gaacgcttca ccatggacga aggcctgtcc aatgcggtga
     9421 aagaagtttt cgttcgtctg tataaagaag acctgattta ccgtggcaag cgcctggtaa
     9481 actgggaccc gaaactgcgt accgctattt ctgacctgga agtggaaaac cgcgaatcga
     9541 aaggttcgat gtggcacatc cgctatccgc tggctgacgg cgcgaaaacc gcagacggta
     9601 aagattatct ggtggttgcg accactcgcc cggaaaccct gctgggcgat accggtgtag
     9661 ctgttaaccc ggaagatccg cgttacaaag atctgattgg caaatatgtc attctgccgc
     9721 tggttaaccg tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg
     9781 gctgcgtgaa gatcaccccg gcgcacgact ttaacgacta tgaagtcggt aaacgtcacg
     9841 ccctgccgat gatcaacatc ctgacctttg acggtgatat ctgtgaaagc gcccaggtgt
     9901 tcgataccaa aggtaacgaa tctgacgttt attccagcga gatcccggca gagttccaga
     9961 aactcgagcg ttttgctgca cgtaaagcag tcgttgctgc ggttgatgca ctcggcctgc
    10021 tggaagaaat taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtggtta
    10081 tcgaaccgat gctgaccgac cagtggtacg tgcgtgccga tgtactggcg aaaccggcag
    10141 ttgaagcggt tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact
    10201 tctcctggat gcgcgatatt caggactggt gtatctctcg tcagctgtgg tggggtcacc
    10261 gtatcccggc atggtatgac gaagcgggta atgtttatgt tggccgcaac gaagaagaag
    10321 tgcgcaagga aaataacctc ggtgccaacg ttgccctgcg tcaggacgaa gacgttctcg
    10381 acacctggtt ctcttctgca ctgtggacct tctctaccct tggctggccg gaaaataccg
    10441 acgccctgcg tcagttccac ccaaccagcg tgatggtatc tggtttcgac atcatcttct
    10501 tctggattgc ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac
    10561 cgcaggtgcc gttccacact gtttacatga ccggtctgat tcgtgacgac gaaggtcaga
    10621 agatgtccaa atccaagggt aacgttatcg acccgctgga tatggttgac ggtatttcgc
    10681 tgccagaact gctggaaaaa cgcaccggca atatgatgca gccgcagctg gcggacaaaa
    10741 tccgtaagcg caccgagaaa cagttcccga acggcattga gccgcacggc accgatgccc
    10801 tgcgcttcac cctggcggcg ctggcgtcta ccggtcgtga catcaactgg gatatgaagc
    10861 gtctggaagg ctaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga
    10921 tgaacacaga aggtcaggat tgcggcttca acggcggcga aatgacgcta tcgctggcgg
    10981 accgctggat tctggcggag ttcaaccaga ctatcaaagc gtaccgcgaa gccctggaca
    11041 gcttccgctt cgatatcgcc gcaggcattt tgtatgagtt cacctggaac cagttctgcg
    11101 actggtatct ggagctgacc aagccggtaa tgaacggtgg aacagaagcc gaactgcgcg
    11161 gtactcgcca tacgctggtg actgtactgg aaggtctgct gcgcctcgcg catccgatca
    11221 ttccgttcat caccgaaacc atctggcagc gtgtgaaagt acttttcggt atcactgccg
    11281 acaccatcat gctgcagccg ttcccgcagt atgatgcatc tcaggttgat gaagccgcac
    11341 tggccgacac cgagtggctg aaacaggcga tcgttgctgt acgtaacatc cgtgcagaaa
    11401 tgaacatcgc gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag
    11461 aacgtcgcgt aaatgaaaac cgtggcttcc tgcaaacgct ggcgcgtctg gaaagcatca
    11521 ccgtgctgcc tgccgatgac aaaggtccgg tttccgttac caagatcatc gacggcgcag
    11581 agctgctgat cccgatggct ggcctcatca acaaagaaga tgagctggcg cgcctggcga
    11641 aagaagtggc gaagatcgaa ggtgaaatca gccgcatcga gaacaagctg gcaaatgaag
    11701 gctttgtcgc ccgcgcaccg gaagcggtta tcgcgaaaga gcgtgagaag ctggaaggtt
    11761 atgcagaagc gaaagccaag ctgattgaac agcaggctgt tatcgccgca ctgtaatcaa
    11821 cacagtgatg aaaacgaagg ccggagcatg ctccggcctt ttttatctct tacagaaatg
    11881 gtaaagaagg cataactccc cgagaaatac tggctaagaa gcctttatct ggttgttctt
    11941 ctttattgat taaaggaagg tcatccaaat cgcccagcaa atacgtattt tttgcctgcc
    12001 agtcaatcat ccatattttc agcagtgggt aagccagacc acccgtaatc ccggatatca
    12061 ccaccaacgc acacatgcga taaagcatac cgtggtacgt taaagttgag cgaaaacgaa
    12121 tacgcccatc attcagtgac aggttgctca taaaatgatt tcgcaacgac accgttaaat
    12181 aactggtgct aacagcaatc ccaaaataat aaattaactg cgcgattatc attttacgtt
    12241 gcatttccat aaattgctgt aaattctcaa tatcatcatt tgcataccca gaactatcat
    12301 actcatttaa tatttgatcg aagataatat aaccagcaac agcgagaaaa ggtaataaag
    12361 ctaaaaatgc gaggatggca tattttatac aatacgcagt atctaatttc accttgaaac
    12421 gatgtatacc gaaactggta ttgctccaga gaaaactcat taccaggcta tataaagtac
    12481 cattaaaaat accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa
    12541 cactacttga actattggca tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc
    12601 caatggccat taaaatcggc aagaaaaagg ttacccacca gaaccctttc aaagagcaat
    12661 taaaactaaa tcttacgccg ttgagactgg tcattaaggc ctgataacgc agtccttttg
    12721 ctgccataaa tataagcaaa accaacagta acaaagtcaa aacacagcca acaagcggca
    12781 tatctgctga cactgtcatt aagattgcga aatagaaaaa aacaaaaaca agacaactaa
    12841 caaaaacatt cccaccggta attccataag aaaatcgttg tccgttaact tccatattag
    12901 cataaagata acgcttacat ttcattaatg cccatggtaa atagatacct agcgttataa
    12961 tcgttaacaa cacattcacc acacaaataa gaaaatatct ctcacctgta ccatgaaaaa
    13021 caaaagaatg ggacggaaca tccatttcat taataacttg agccataaaa atcccctgaa
    13081 ttaaaaaatc cattgatata atttatagat acagacagta aaatgttctg ccacgttaca
    13141 atactcttat cagtaatagg ctggcaaaaa ctttattgct ttataagacg cttgcgcacg
    13201 taagaagaga gtggtattaa acaaatgtat aattacttta tttttattgg gttaaactat
    13261 gaataacatt gcgccgcaat cacctgtaat gcgtcgcctg acgctgcagg ataatcctgc
    13321 tatcgccagc gtcatccgtc aggtttacgc agagtatggt cttaccgccg ataaaggcta
    13381 caccgttgcc gatccgaatc tcgacgaact gtatcaagtg tacagccagc ctggccatgc
    13441 ttattgggtg gttgaataca atggcgaagt ggtcggcggc ggcgggattg cgccattaac
    13501 aggtagtgaa gcggacattt gcgaactgca gaaaatgtac tttcttcccg ctattcgcgg
    13561 taaagggcta gcgaaaaaac tggccttaat ggcgatggag caggcacgag agatgggctt
    13621 caaacgctgc tatctggaaa cgaccgcttt tttaaaggaa gccattgcgc tttatgagca
    13681 tttgggcttt gagcatatcg actatgcgct tggctgcacg ggccatgtcg attgtgaagt
    13741 gcggatgcta cgcgaactct aaaaaaatgc ccgctagtat gtgctatcgg gcattttaat
    13801 caattaatta gtggcgaacc ccgtcatcgt cttcatcgac aaaatcttca tcgtcgccat
    13861 cttcgccgtt aggatcttca aagtaagtgc cccaaccgtc gtactcaacg tcaaatttct
    13921 ccgccagcgt catcagttgt tcaacctggg catcgatcag atcggcattc aacgcgcact
    13981 cgctgaggat gtcgcagcaa atcacgatat caccatcttc aacttccagc tcttctggat
    14041 cggtcacttc gtaaccgagt ttaaacgctt caactgccgc tttttccaga gtttccagat
    14101 cgtctgcgga aagatggtgt tcgatggtgt acagcgcgtc cggatcgctg ccatcttcca
    14161 gtaattcttc aataatcaaa cgtgtttctt cacgctgttc ttccagttgt tccgggtttg
    14221 ccatggctca ttcctcttaa tgtcctgccg atactcttat tgtcacacac cactgacatt
    14281 gcctccacct ttgtgacaaa gatttatgct ttagacttgc aaatgaataa taatacacat
    14341 aaattgaatt ttaattcatt gaggcgttag ccacaggagg gatctatgtc cgggttttat
    14401 cataagcatt tcctgaaatt actcgatttc acgccagctg aactcaacag cctgctgcag
    14461 ttagccgcga agctgaaagc tgataagaaa agcggtaaag aagaagccaa actcactggt
    14521 aaaaacatcg cgctcatctt cgaaaaagac tcgactcgta cccgatgctc tttcgaagtt
    14581 gccgcatatg accagggggc tcgcgttact tatcttggcc caagcggtag tcagattggt
    14641 cataaagagt cgattaaaga cactgcccgc gtgcttggtc gcatgtatga cggtattcag
    14701 tatcgcggct atggtcagga gattgtcgaa acactggcgg aatacgctgg cgtgccggta
    14761 tggaacggcc tgaccaatga gtttcacccc acgcagttgc tggcggatct cctcaccatg
    14821 caggagcatt tacccggtaa agcattcaac gaaatgacgc tggtctatgc aggtgacgca
    14881 cgtaacaaca tgggcaattc gatgctcgaa gctgcggcgc ttaccggtct ggatttgcgt
    14941 ctggtcgcgc cacaggcatg ctggccagaa gctgcgctgg ttacggaatg ccgcgccctg
    15001 gcacaacaaa atggcgggaa tattacgctg acggaagatg tagctaaagg agttgaaggt
    15061 gctgacttta tctataccga tgtatgggtg tcgatgggtg aagcaaaaga aaaatgggct
    15121 gaacgcattg cattactgcg tgattatcag gtgaacagca agatgatgca gttgaccggt
    15181 aacccggagg tcaaattcct ccattgcctg cccgcgtttc atgacgacca aacgacgctt
    15241 ggcaaaaaaa tggcggaaga gtttggccta catggcggaa tggaagtgac tgatgaggtc
    15301 ttcgaatctg ccgccagcat tgtatttgat caggcggaaa accgcatgca taccatcaaa
    15361 gcggtgatgg tcgcgacgct cagtaaatta aataattaat atctctataa a
//
LOCUS       DAJTRB010000090        15345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTRB010000090 DAJTRB010000000
VERSION     DAJTRB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.090"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO330_003172"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO330_003172"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5883945.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO330_003173"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO330_003173"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5883946.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO330_003174"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO330_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5883947.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO330_003175"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO330_003175"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5883948.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO330_003176"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO330_003176"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5883949.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO330_003177"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO330_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5883950.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYTIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO330_003178"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO330_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5883951.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO330_003179"
     CDS             complement(8065..9207)
                     /locus_tag="OO330_003179"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5883952.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO330_003180"
     CDS             complement(9216..10229)
                     /locus_tag="OO330_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5883953.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO330_003181"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO330_003181"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5883954.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO330_003182"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO330_003182"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5883955.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO330_003183"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO330_003183"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5883956.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO330_003184"
     CDS             complement(12827..13951)
                     /locus_tag="OO330_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5883957.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO330_003185"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO330_003185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5883958.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4381 a         3447 c         3144 g         4373 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg aaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgc cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgccaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat actatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaacatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
    15121 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattttc
    15181 aataaagaaa tcaatcaaat aaaatcatta gcaaccatat tctttcactg ttgctctgcg
    15241 attccttcct gcacgtacag cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata
    15301 gatgttcagc aagaattggc gggtaaattg ctatttaagt caaaa
//
LOCUS       DAJTRB010000091        15202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTRB010000091 DAJTRB010000000
VERSION     DAJTRB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.091"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..684)
                     /locus_tag="OO330_003186"
                     /pseudo
     CDS             complement(1..684)
                     /locus_tag="OO330_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            complement(1087..1332)
                     /locus_tag="OO330_003187"
     CDS             complement(1087..1332)
                     /locus_tag="OO330_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883959.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1977..2052)
                     /locus_tag="OO330_003188"
     tRNA            complement(1977..2052)
                     /locus_tag="OO330_003188"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2017..2019),aa:Phe,seq:gaa)
     gene            complement(2158..2865)
                     /locus_tag="OO330_003189"
     CDS             complement(2158..2865)
                     /locus_tag="OO330_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5883960.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3263..5398
                     /gene="speC"
                     /locus_tag="OO330_003190"
     CDS             3263..5398
                     /gene="speC"
                     /locus_tag="OO330_003190"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5883961.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5447..6703)
                     /gene="nupG"
                     /locus_tag="OO330_003191"
     CDS             complement(5447..6703)
                     /gene="nupG"
                     /locus_tag="OO330_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5883962.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6905..7984)
                     /gene="mltC"
                     /locus_tag="OO330_003192"
     CDS             complement(6905..7984)
                     /gene="mltC"
                     /locus_tag="OO330_003192"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5883963.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8049..8324)
                     /locus_tag="OO330_003193"
     CDS             complement(8049..8324)
                     /locus_tag="OO330_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5883964.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8352..9404)
                     /gene="mutY"
                     /locus_tag="OO330_003194"
     CDS             complement(8352..9404)
                     /gene="mutY"
                     /locus_tag="OO330_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5883965.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9565..10284
                     /gene="trmB"
                     /locus_tag="OO330_003195"
     CDS             9565..10284
                     /gene="trmB"
                     /locus_tag="OO330_003195"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5883966.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10284..10610
                     /locus_tag="OO330_003196"
     CDS             10284..10610
                     /locus_tag="OO330_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5883967.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10794..11513
                     /locus_tag="OO330_003197"
     CDS             10794..11513
                     /locus_tag="OO330_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5883968.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11689..12735
                     /gene="ansB"
                     /locus_tag="OO330_003198"
     CDS             11689..12735
                     /gene="ansB"
                     /locus_tag="OO330_003198"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5883969.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12852..13814
                     /locus_tag="OO330_003199"
     CDS             12852..13814
                     /locus_tag="OO330_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5883970.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13915..>15202)
                     /locus_tag="OO330_003200"
     CDS             complement(13915..>15202)
                     /locus_tag="OO330_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5883971.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTT
                     SIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMT
                     PAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQH
                     LIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3936 a         3665 c         3923 g         3678 t
ORIGIN      
        1 ggttcgggcc actggcgtaa aaatcctgct ccaacagatt ttctgacaca ggcaggccgt
       61 gtgcgcggta gctgaccggg ctgaacttcc gggaggcctt tgccctcagt ccctgacggc
      121 gcaggcttgc cgccacggtt tttacgttaa aggggtaacc ctgagcacgc agttcatccg
      181 tcaggcgtgg ggcaccgtaa cgctgttttg accgggtaaa agccgcgagg acaacgctgt
      241 cgcagtgttg gcggaactgc tgacgcgtgc ttatccttgt ccgccgctga caccacgtat
      301 accagccgct gcgggccacc cggagcacgc ggcacattgc tttgatgctg aactcagcct
      361 gatgtttttc aataaagaca tacttcattt caggcgcttc gcgaagtatg tcgcggcctt
      421 ttggaggata gccagctctt catcccgttc tgccagctgg cgtttgagac gtgcaatctc
      481 ggtagacatc tccagttcac gttcagaaga cgtctgctga ttttgctgtt tactgcgcca
      541 gttgtagagc tgtgattcat acaggctgag ttcacgggct gcggcagtaa caccgatgcg
      601 ttcagcaagc ttcagggctt cactgcgaaa ttcaggcgaa tgctgtttac ggggtttttt
      661 actggttgat actgtttttg tcatgtgagt cacctctgac tgagagttta ctcacttagc
      721 cgcgtgtcca ctattgctgg gtaagatcag gctgaggagt ccccagaaaa gaagacaggc
      781 atagagtttt gtgatctact gattgcgcta acaaattcaa tgagatcacc tctatgcctg
      841 cccgttaagt atgtcaggct tttttccgta atgctctaac ttccacgcat tggtttctga
      901 catcaaataa ggctatgact ttctctccct tttaatctca ttctgatcta aaaccatatc
      961 ttaaaaaaat gcatgggtat tttaattatt ttgatgttga tctaacgcac tcaaggacca
     1021 ttttattacc gattatccat tctatttaac tgttgcgata taaatgtttg ttttaggtgc
     1081 tttaattcag tacaagtcat caaaccgtac cagaccctga ctgtcagtga caccgacagg
     1141 tgggggcggc ggtgtggggt caaactgagt gtgtctgcct ctggcccaca gagccagctc
     1201 tgccgccatt gcccgtggat gatactccag ctgcacgttg agaccggaaa ctgggttgtt
     1261 gttctcatcc acgaactgca gcgccagcca gtgcatccgc tgcttaccgc aggcgacttc
     1321 tttgtacttc attttgtgac tcaggcaggt gactgtcgtg ttgcctgttg cgtccatttt
     1381 gcggttaaac ccttttaatt caatttgtta aattgctttc caccttttgc ggggcgcgac
     1441 ttaaccaaaa tattcactgt tatcataata tgcaatgggc tggaggttgc aggtcattta
     1501 gggattttct gtaagccccg ttttgcagaa cgttctatct ccgattgaga tcctgtctct
     1561 cggtattacc ggtagtctgg ccataatccc ctatggatca tattgatata gtaggcataa
     1621 cacccaaaag ttgactaaag ctaaatcaat cagagtgaaa aatgttcggt ttgatgtagg
     1681 gggggactga ggaggggcca acgcaggtgg tatgcgtttt acttcagacc ggacaaagac
     1741 cggatgtatc cgtgcagtaa tgaatccgtt agccaggtgc tcatgtggaa atagtgtcta
     1801 ttggggattt gaagtttgac agaaaaggcc acagtgacca gaagggggct acttagaaga
     1861 aatgttcagg ctggatggaa gaggtaaagc agtgacatca gcattcaaag tgaccggata
     1921 tctaccagaa ccccagaaag caaaaaacca gcccgtaggc tggttcttct aaatagtggt
     1981 gcccggactc ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg
     2041 agctatccgg gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc
     2101 ttcaatttac tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca
     2161 ggcaaaccag gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat
     2221 gttgaccacc gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa
     2281 accgcctacg gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc
     2341 ggcagcccac gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc
     2401 aatacccagc gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat
     2461 taaaatactc ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct
     2521 ggcgcaaaac aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt
     2581 cttacgtgag tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc
     2641 cagcaaacaa atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag
     2701 gttggcgcat ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga
     2761 tgtcatggag acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc
     2821 acccagtaag actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag
     2881 ctgtattgtt tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc
     2941 aaagtcctgg cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc
     3001 tgtacggagt ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg
     3061 cgcgaaacgt ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg
     3121 caggacaaca gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg
     3181 tgaccagttt gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact
     3241 tccccgaaac gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt
     3301 atcccgactt tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt
     3361 cgcggcagtc gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg
     3421 caccggtttt catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg
     3481 cgttacggcg gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg
     3541 tcagtatgaa gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat
     3601 gggcaacagc acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc
     3661 tgccggacgc catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa
     3721 cgctgacgta aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa
     3781 attcgcagcc aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc
     3841 agcgaataaa gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg
     3901 taacaaccat aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta
     3961 tctggaagct tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa
     4021 tgaagagtat ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg
     4081 cccgtttcgc ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg
     4141 tcaggtgatc gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt
     4201 cggttacgaa cagtttatcc cgatgatggc ggatagctcg ccgctgctgt tagaacttaa
     4261 cgaaaacgat ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc
     4321 acagacgtcg cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc
     4381 gcataagcgg ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct
     4441 gtttgctgca ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg
     4501 ggctgagtgc gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct
     4561 gttccgcccg tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc
     4621 agtgttagcc agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt
     4681 tgaaggatat gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc
     4741 aggtatcaat gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc
     4801 gcactatctg cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt
     4861 cttattaact ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca
     4921 atttgaacag catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa
     4981 caaatatccg gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga
     5041 tctgtatgtc agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt
     5101 cccgtcagtg gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga
     5161 gttggtgcgg attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc
     5221 acctggcgtg ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt
     5281 ccttgcgctg gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt
     5341 ctatagcgaa accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga
     5401 ataaaaaaac gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg
     5461 tctgtgtgcc tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg
     5521 ccagaaccac ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt
     5581 tttgggtgta catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc
     5641 cgttagtcat catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt
     5701 tttcgacaaa caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca
     5761 tcgacagtac cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa
     5821 aacgcaggat ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta
     5881 agaagaacgg gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga
     5941 ttgacgcatg ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca
     6001 ggaaggtatt accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga
     6061 tgaagaagat tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg
     6121 ttgtccagct ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa
     6181 acagaaccag aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga
     6241 agccagacag gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga
     6301 ttggcgggaa gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg
     6361 tgttgattaa cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca
     6421 tcgcttccgg cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc
     6481 aaatggcata tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag
     6541 gcataaatac cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa
     6601 acttcagggt aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga
     6661 actgcagaaa agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc
     6721 gtgatgcgga tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc
     6781 aacttcgcgg actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc
     6841 aaaacgaaat tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat
     6901 ggaattatcg gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag
     6961 attccgcaga gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt
     7021 taataatatt ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc
     7081 cgccgttata ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga
     7141 gataaacatt gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat
     7201 caaacaagaa actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc
     7261 cggcagtatt ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg
     7321 ggttaaagga agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt
     7381 tccgtgacgc ctggcggacc ataccgagat atttgtgcgc acgtttatca aggtggttcg
     7441 gcaccatgtt aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac
     7501 ggtttttcag cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc
     7561 cggtgttgtc caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa
     7621 cgtcggaata gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg
     7681 cccggcgcaa atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt
     7741 catcgaagtt gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag
     7801 gaccggcgat caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc
     7861 ccatcagaat atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt
     7921 ttttggtcgt cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt
     7981 tcatcattcc gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg
     8041 cactgttttt attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg
     8101 aacaggaagt tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg
     8161 agtttctttt cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg
     8221 gagatctcgt tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct
     8281 tctgcttcac gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat
     8341 cgactcacgc gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga
     8401 gccgccaggc caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc
     8461 atgcagccgg tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg
     8521 ctgaaggtat ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt
     8581 gccagccaat gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc
     8641 cacaatccgc tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga
     8701 aagtagccgg tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag
     8761 ctattgttgg tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc
     8821 gaacgcgtgc aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg
     8881 accgcgggcg tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca
     8941 ggccagccgc ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc
     9001 ggaaagtgct tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc
     9061 ggtaacgccg cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact
     9121 tgttgtgccg ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag
     9181 tggagaactt cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata
     9241 aagcgttcaa aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag
     9301 agccatactt tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta
     9361 tcgtaccagt ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact
     9421 gttgttgggg gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag
     9481 ccacggcctt atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac
     9541 actttcacaa gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa
     9601 aacggccgcc cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa
     9661 ggccaggaac atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat
     9721 atgctggatt tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt
     9781 ggcatggggg cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc
     9841 attgaagtgc attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta
     9901 agcaacctgc gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac
     9961 aattcattgc gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat
    10021 aaacgccgta tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg
    10081 ggcgtattcc atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg
    10141 tcttctattg acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca
    10201 tcacgtccgg tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac
    10261 ttaatgttcg agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa
    10321 tgcacatcga cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta
    10381 catcggaaga acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga
    10441 acaaactggc ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg
    10501 aaatcggcaa atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca
    10561 aactggatga ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac
    10621 gggcgatgac aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca
    10681 cgatcgagta ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg
    10741 ttatcaatct ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc
    10801 gcaaaatgct gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc
    10861 agtgcagcgt cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg
    10921 gcgaaaacgg caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat
    10981 attccctgaa tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca
    11041 cgctgccgtg gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg
    11101 ataaaattat cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac
    11161 ttgatgcgca gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga
    11221 cgttccacta taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg
    11281 caatgggcgg aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa
    11341 gcggcggtaa cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc
    11401 aaaccgagtg gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc
    11461 gcgttgtgac tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta
    11521 ttttaagacg gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct
    11581 aactttgtag atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat
    11641 gcaaagctaa gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc
    11701 aaaaagacgg cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc
    11761 aatatcacca ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa
    11821 tctaactaca cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta
    11881 aaagacattg cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac
    11941 gataatgtct ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc
    12001 ttcgtcatta cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg
    12061 gtgaaatgcg acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc
    12121 gcagacggtc cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct
    12181 aatcgtggcg tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa
    12241 accaacacca ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt
    12301 cacaacggta agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca
    12361 ttcgatgtct ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac
    12421 gcatccgatc ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct
    12481 ggtgtgggta atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa
    12541 aacggcactg cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct
    12601 gaagtggatg atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg
    12661 cgcgtcctgc tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt
    12721 ttcaatcagt actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt
    12781 acgtccattg ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg
    12841 agggttttcg ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg
    12901 gtaatgcctg ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa
    12961 agcagtatca cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg
    13021 gtaatcaggc cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct
    13081 gggtcggtca gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg
    13141 taaaattctc ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact
    13201 tttactcttt tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga
    13261 ataataaata tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat
    13321 ctgcgttgaa tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg
    13381 ataagcaaat ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg
    13441 gtaagcaaat gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta
    13501 ataaacagaa cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg
    13561 cgattgcggc atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac
    13621 gagatttcga tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc
    13681 gtaaattgca ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag
    13741 tttcagaaat aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag
    13801 ctaatgaacg ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac
    13861 tcatgttaag tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat
    13921 tagccctagt agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa
    13981 cgctactagc aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac
    14041 acccgagata gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat
    14101 acaaaggttg gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc
    14161 aatcggcaag aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc
    14221 gacaattaat aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc
    14281 catcaatgcg tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc
    14341 cacggaaaaa ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag
    14401 tttgggtaaa tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta
    14461 gacaacggca attgctgacg cttcagttgc ggtaaaaatg ccgcccatca caatgaacac
    14521 cagaagcaaa cttggtaagg catccagaaa cgctttacag gcctgagcaa aggttgctct
    14581 ttctgaaagc gggtagccac ggcgtttagc aataatgccg cagactatca ttaccgcaag
    14641 acccattaag atccctggca aatatcccgc cataaacagc gatgctacag aaacgccgcc
    14701 agctgtcagg gaaaacacaa ttaacacgtt tgagggcggt atcagtaatc cagtaataca
    14761 agatgagacg tttacagcgg tagaaaatgc cggatcataa ccttctttgg tctgaatggg
    14821 attcagtgtg ccaccaacgg cagcagctgc cgcaacggct gaaccagaaa tggaaccaaa
    14881 catcatattc gccaggacgt tcacatgacc aagcgaacca ggaacgcggc caaccattac
    14941 ctgggcgagg ttaatcaaac gtatcgcaat accgccgctg ttcatcagtg ttccggcaaa
    15001 gatgaaaaag gggattgcca acagggcaaa gttatccagt ccattagcca gtcgttgcga
    15061 aacagtgatt gccgcgatat cccagggaaa catcaaaagc attgaagcta cagtggcaat
    15121 accgattgag aacgaaatgg gcacaccaat aaagaccagc acaaaaaaac agccaaacag
    15181 tgtaatcgcg atataactat cc
//
LOCUS       DAJTRB010000092        15085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTRB010000092 DAJTRB010000000
VERSION     DAJTRB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.092"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..237
                     /locus_tag="OO330_003201"
     CDS             <1..237
                     /locus_tag="OO330_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5883972.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="OO330_003202"
     CDS             247..885
                     /locus_tag="OO330_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5883973.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="OO330_003203"
     CDS             888..2051
                     /locus_tag="OO330_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5883974.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..3760
                     /locus_tag="OO330_003204"
     CDS             2135..3760
                     /locus_tag="OO330_003204"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5883975.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="OO330_003205"
     CDS             complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="OO330_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5883976.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="OO330_003206"
     CDS             complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="OO330_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5883977.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4812..5561
                     /gene="yjfP"
                     /locus_tag="OO330_003207"
     CDS             4812..5561
                     /gene="yjfP"
                     /locus_tag="OO330_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5883978.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="OO330_003208"
     CDS             complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="OO330_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5883979.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="OO330_003209"
     CDS             complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="OO330_003209"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5883980.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7840..9237
                     /gene="ulaA"
                     /locus_tag="OO330_003210"
     CDS             7840..9237
                     /gene="ulaA"
                     /locus_tag="OO330_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5883981.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9253..9558
                     /gene="ulaB"
                     /locus_tag="OO330_003211"
     CDS             9253..9558
                     /gene="ulaB"
                     /locus_tag="OO330_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5883982.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9568..10032
                     /gene="ulaC"
                     /locus_tag="OO330_003212"
     CDS             9568..10032
                     /gene="ulaC"
                     /locus_tag="OO330_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5883983.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10046..10696
                     /gene="ulaD"
                     /locus_tag="OO330_003213"
     CDS             10046..10696
                     /gene="ulaD"
                     /locus_tag="OO330_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5883984.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10706..11560
                     /gene="ulaE"
                     /locus_tag="OO330_003214"
     CDS             10706..11560
                     /gene="ulaE"
                     /locus_tag="OO330_003214"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5883985.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            11560..12246
                     /gene="ulaF"
                     /locus_tag="OO330_003215"
     CDS             11560..12246
                     /gene="ulaF"
                     /locus_tag="OO330_003215"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5883986.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12375..12650)
                     /locus_tag="OO330_003216"
     CDS             complement(12375..12650)
                     /locus_tag="OO330_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5883987.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12977..13372
                     /gene="rpsF"
                     /locus_tag="OO330_003217"
     CDS             12977..13372
                     /gene="rpsF"
                     /locus_tag="OO330_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5883988.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13379..13693
                     /gene="priB"
                     /locus_tag="OO330_003218"
     CDS             13379..13693
                     /gene="priB"
                     /locus_tag="OO330_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5883989.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13698..13925
                     /gene="rpsR"
                     /locus_tag="OO330_003219"
     CDS             13698..13925
                     /gene="rpsR"
                     /locus_tag="OO330_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5883990.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13967..14416
                     /gene="rplI"
                     /locus_tag="OO330_003220"
     CDS             13967..14416
                     /gene="rplI"
                     /locus_tag="OO330_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5883991.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14487..15050)
                     /locus_tag="OO330_003221"
                     /pseudo
     CDS             complement(14487..15050)
                     /locus_tag="OO330_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         3524 a         3678 c         4068 g         3815 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccaataa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gttccggcaa
     3421 tattatgtgc ctggatgtgt tgcgcgttct caataagcaa gcgggcgtat acgacttatt
     3481 gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg cggttcgtcg
     3541 tttacagcag cagctgcgta agccagctga agaactgggg cgagagatta ctcatcagct
     3601 attcctgctg ggctgcggtg cgcaaatgtt gaaatatgct tctccgccaa tggcgcaggc
     3661 gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc agatccagaa
     3721 tgatttattg ctgcgggcga cggggggagt gtgtgtgtaa gcgtatacga ctgatgcgac
     3781 gctggtttcg attaactaaa tgaaatatgt gaaaattgta ggccggacaa ggcgctcgcg
     3841 ccgcatccgg cattgttcat ttgctgtaga tgacactcat gcatacagta tcgcctgtac
     3901 gcgccagtta tcaggctgct ggtcttcgta catggtaata atgcggtacc atgacgcacc
     3961 ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc tgcggatccc ctgaaatatt
     4021 gctaactgag atctgaccta tttcattcag gcccgtcacg cagtcagcac tggcgaattc
     4081 tgcagactgt gccgtggcgc tcgcaggata agtcactaat tgcagcgagg gtgaggcaag
     4141 taattgtttc atcgtcagct ccatttttcg tttccccaca tgttgcaggg cattccatcg
     4201 cggtaataaa cgcccaagct tccagccggt aatttattgt gcacaggtaa acgcataggg
     4261 acgcaaagca atgtaaagct gactatgtaa gttgtctgat ttatttacgg tacaaaatgg
     4321 cttgtgaata ccactgtcct gtcacgatgg tttcgtccac catgacgaca acgtaataat
     4381 cagcttttgc agcgacagct tttgctctga tttctgctaa tgcgtcatcc ggagaacccc
     4441 gaaccatcgt gcttacgcta cctattcgct gtaacccttg cgtctggtcg cggcgaatct
     4501 cttgcggatg gtccgttact ggcggtgctg gctgtggcgt accttgcagt gcgctacagg
     4561 cacttaacat caacaccaat aataaactgg caaaccggta aataacgcta ttacgtttcc
     4621 tgctaaccat agtgtagtgc cttattaatt taactttggg ggaatgttcc cgaattgtta
     4681 cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc tcaacattat
     4741 gaactaatac cgccagcaga aacttgtgct aaggatttct tcatgtcata ccagacaaaa
     4801 aggagagaca gatgattgaa atagaatcac gcgagctggc agatattccc gttcttcatg
     4861 cttatcctgt cgggcaaaaa gataccccgt taccgtgcgt aattttttat cacggcttta
     4921 cttcatccag cctggtgtat agctattttg ccgttgcgct ggcgcaggct ggtttgcggg
     4981 tgatcatgcc ggatgcgccc gatcacggta gccgttttag tggcgacgaa gcgcggcggt
     5041 taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga attcactact ttacgtgcgg
     5101 caatagccga agaaaactgg ctgcttgatg accgtctggc agtcggtggc gcgtcgatgg
     5161 gcgcgatgac ggcactggga attaccgctc gccaccccac ggtgaaatgt accgccagca
     5221 tgatgggatc gggctatttt acatcgctcg cccgttcact gtttccaccg ctgatacctg
     5281 aaacggcagc acagcagaat gaattcaata aaattgtcgc gccgctggca gagtgggaag
     5341 cgacaaacca cctggaacaa cttggggaca gacctctact gctgtggcat ggcctcgacg
     5401 atgacgttgt gcctgccgac gaatcactac gtttgcagca ggccttaagc gagacgggac
     5461 gggataaact gctaacctgt tcatggcagc caggcgtgcg tcaccgtatt acgcctgagg
     5521 cgttagatgc tgccgtgaca tttttccgcc agcatcttta aacacgcaga atgctgaccc
     5581 cttgcgcttc cagttgttgc aggatttccg ggttagcatt tttgccggtg atcagcatat
     5641 cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc gccaatctta ctgctatcaa
     5701 ccagtaccac cagtttccct accacgctca gcatcttctg ctctgccatt gctgtcagca
     5761 tatcggtttt atacaaccct tctgcggtca gcccttttcc gctggtaaac atccagtgcc
     5821 cggcatagag actgttttcg ctgccctgcg ggcttaaagt gatggactga cttttgttgt
     5881 actgtccacc cataatgatc acgctgtcat gttcttgatc gatcaggtaa tttgccagcg
     5941 gtagataatt agtgatgatt tgcactggct tgccacacat ttcccgccca agcagaaacg
     6001 cggtggagcc gcagttgatg actacgcttt cgcccggatt aaccagctgc gaggccgctt
     6061 tagcaatacg tactttttca tcgtgattct gcgcctgatg cagattcatc ggcgtccagc
     6121 gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac ctttttcagt ttgccgcttt
     6181 cgccaagttt attgatatcg cgtcgcgcag tggcaggcga aattcccaga cgctcaacga
     6241 ctttctcaac ggtcacaaag cccaattgtg cgagcatttc caggaggatt tgatgtcttt
     6301 gtgcttcagt catgagctat tccgataaaa tttgattttt ttagatgata tttgaaatag
     6361 ccaggaaata ctacgccgga tagcgcgaac ttctccacgc tatccggtat aaacgacaga
     6421 ttacaggaat gacttgaacg gcagatccgg ttcaatagtg aagcaatcat cgaaaccgcg
     6481 cggatagtgg tactcgaagt tgtctttatc cagcggccag gtaaatttgc cgccaacctg
     6541 ccagataaac ggcttgaagc catacttcag acgatctttt ttgatctccc acagcacgcg
     6601 gatctcttgc ggatcggcct ggaagtttga ccagatgtcg tggtggaacg ggataaccac
     6661 tttcgcattc agggcttcac ccatacgcag catatcggcg ctggtcattt tgtcggtaat
     6721 accgcgcggg ttctcaccat acgatcctaa cgccacatcg atctgatgtt cgttaccgtg
     6781 cttcgcataa tagttagagt agtgggaatc gccactgtga tatagggaac cgccaggagt
     6841 tttgaacagg tagtttactg cgcgatcgtc catgccatct ggcagtacgc cagccgcttt
     6901 ttgatcggca ggcagggtga tcagtgcagt acggtcgaaa gcgtcaagcg catgaatttc
     6961 aatgtctttc acttttacaa catcgcccgg tttgaccacg atgcaacgct ctttcggtac
     7021 gccccagcca atccacaaat caacgcaggt tttcggtcca atgaatggta cgtcatcagc
     7081 acagttctgc atcacggcag cagcgacgtt aacgtcgata tgatcgttgt gatcgtgagt
     7141 cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg gggtggtgcg
     7201 taggtttggc tgcagttttt tcacgccagc catgcgctgc atctggtgac cctgtttcat
     7261 taacgggtta ccgtgacttt gtttgccagt gccgcaccag aaatcaacgc aaacgttggt
     7321 gccaccttct gatttcaacc agatcccggt gcagcctagc caccacatcg caaatgtgcc
     7381 aggggcgacc tgctcttgtt caatttcttc attcagccag ctaccccact ccgggaaagt
     7441 gctcaggatc caggattctc gggtgatact tttcacttta ctcatcgcca tttaccttca
     7501 tgatagttca attcgaatca acatgtgatt ggttttgatt aatcctgaca ctattccttc
     7561 aggaaggcaa tgaccatttt ttgacttttt gccagggaag ttgttgttga ttttgagtgt
     7621 ggaaagattt aatggaatgt gtagttaaat taactaaatg aatttaaata aataattgtt
     7681 tcgttgtgtg aatcacactc tatccgtgtg gaattatttg cgggtcgcgt cacatttaat
     7741 cataaataat cttgttgtga ttacttttga aaattagagt gagtgcacaa cattccgggt
     7801 gtgcggaata cccggttacc tcttcttcag gagatcgtta tggagatcct ctacaacatc
     7861 tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg tattgtgacc
     7921 tgtctgggct acatcctact gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa
     7981 accataattg gtttcatgtt gttgcaggca gggtccggca tcctcaccag caccttcaaa
     8041 ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc cgatacttac
     8101 gcgtcgatga tggcaaccat cgaccgcatg ggcgatgcct atagctgggt aggttacgcg
     8161 gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt
     8221 cgtaccatca tgctgactgg tcatatcatg ttccagcagg cggggctgat tgccgttacg
     8281 ttgtttattt tcggctactc catgtggaca accattatct gcacggcgat tctggtttcg
     8341 ctctactggg gcattacctc caacatgatg tacaagccga ctcaggaagt gacggacggc
     8401 tgcggtttct ccatcggtca ccagcaacag tttgcatcat taattgccta taaagttgcg
     8461 ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg ctggctgaat
     8521 attttccacg acaacatcgt ctccacggcg attgtcatga ccatcttctt tggtgccatt
     8581 ctgctctcct tcggtatcga caccgtgcag gcgatggcag gcaaagtgca ctggacggtc
     8641 tatatcctgc aaaccggttt ttccttcgcg gtggcgatct tcatcatcac tcagggtgtg
     8701 cgcatgtttg tggcggaact ctctgaagcg tttaacggta tctcccagcg cctgatccct
     8761 ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgctccgaa cgccgtggtc
     8821 tggggcttta tgtggggcac catcggtcag ctgattgcgg ttggcatcct ggtcgcctgc
     8881 ggttcctcga tcctgattat cccaggcttt atcccgatgt tcttctccaa cgccaccatc
     8941 ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg
     9001 gggatgatcg aaatctttgg ttgcgtctgg gtggtgaaac ttaccggtat gagtgcctgg
     9061 atggggatgg cggactggtc gattctggca ccgccgatga tgcagggctt cttctccatc
     9121 ggtatcgcct ttatggccgt catcattgta attgcactgg cttatatgtt cttcgctggc
     9181 cgcgcactgc gcgcagaaga agatgcagaa aaacaattgg cagaacagtc tgcttaataa
     9241 ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca aggcagttcc
     9301 atgatcatga agatgaaagt ggaccagttt ttaacccaat caaacattga ccacacggta
     9361 aatagctgcg cggttggcga atacaaaagc gagttgagcg gcgcggatat catcatcgct
     9421 tctacgcaca ttgcgggcga aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc
     9481 aacatgctct ctcctgccga ctttggcccg aaactgctgg aagtgatcaa agcgcatttc
     9541 ccgcaggatg tgaagtaagg acgcgccatg aaattacatg attcgctggc ggagaataaa
     9601 tccatccgcc tgcaggctga agctgagaca tggcaggacg cagtaaaaat cggcgttgac
     9661 ctgctggtgg cggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt
     9721 gaacagcacg gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg
     9781 gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc
     9841 aaccacgaag ataacgaccc ggtggatatc ctcatcacca tggcggcggt cgatgccaac
     9901 acgcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc
     9961 gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac
    10021 gcggcagctt aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa
    10081 ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat
    10141 cgaagtgggt accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc
    10201 gctctacccg cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct
    10261 ttctcgtatg tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat
    10321 caacaccgcc aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga
    10381 actgaccggt tactggacct gggaacaggc gcaacagtgg cgcgacgcag gtattcagca
    10441 ggtggtttat caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga
    10501 tatcactgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct
    10561 ggcgctggaa gatctgccgc tgttcaaagg cattccaatt catgtgttta tcgccggtcg
    10621 cagcatccgt gatgccgctt ctccggttga agccgcgcgt cagttcaaac gttccatcgc
    10681 cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga
    10741 aaaagcgctc cccgccgggg agtgctggct ggaacgcctg cgactggcaa aaacgttggg
    10801 cttcgatttt gtcgagatgt cggtagatga aactaacgag cgcctgtcgc gcctcgactg
    10861 gagccgcgaa cagcgtctgg cgctggtgaa tgctattgtt gaaaccggcg tgcgtgtgcc
    10921 gtccatgtgc ctttctgccc atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg
    10981 ggcgcagggg ctggagatta tgcgtaaagc catccagttc gcccaggacg ttggcattcg
    11041 cgtgatccag ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg
    11101 tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc aggtgacgct
    11161 ggcgatggag atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc
    11221 gcactatctc aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg
    11281 ggacaacgat gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt
    11341 gaaagacacc aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt
    11401 cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat
    11461 gtggagcgaa acggcggaag acccggcggc agaagtggtg aaagcgcgtg attgggtgaa
    11521 agcgcgcatg gccaaagcag gcatggtgga ggcggcataa tgcaaaagct aaaacagcag
    11581 gtgtttgaag ccaacatgga tctgccgcgc tatgggctgg tgacctttac ttggggcaac
    11641 gtcagcgcta tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgttgcctac
    11701 gaaaccatga aagcagacga tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg
    11761 gagtatcgcc catcttccga cactgcgacg catctcgaac tctaccgtcg ttacccgtcg
    11821 cttggcggca ttgtccatac ccactccact catgccaccg catgggcgca ggcggggctg
    11881 gcgatcccgg cattaggcac cacgcacgct gactacttct ttggtgatat cccgtgtacg
    11941 cgtggattaa gcgaagaaga ggtgcagggt gagtatgaac tgaacactgg caaagtgatt
    12001 atcgaaacgc tgggcaacgc cgagccgctg catacgccgg gaattgtggt gtatcagcac
    12061 ggaccgttcg cctgggggaa agatgcccac gacgcggtgc ataacgcggt agtgatggaa
    12121 gaagtggcga aaatggcgtg gattgcgcgc agtattaatc cacaactcaa tcatatcgac
    12181 agctacctga tgaataaaca cttcatgcgt aagcacggtc ctaacgctta ttacgggcag
    12241 aagtaaaaca cgcgctgcgg aaatttcctt cctcgggaga taactggtct aattccgcag
    12301 ccgtttttca aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt
    12361 ggttatacct ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc
    12421 ggttcccgca tcagaattac gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt
    12481 aatgcctgac gtgcttcgct ttcagtggcg aaattatgat tgatataaat cacgcctaag
    12541 ctttgcacat cgtccatatt tctggcctgg tggttattaa tttcaatggc tgcccatgta
    12601 tttgcactta gcaaaagcac agccagaagg gctaaaactc gactgaacat agatacctcc
    12661 tcgacggctg actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat
    12721 gataaagaag ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ctgtgacgga
    12781 atgtgatgaa cttcaaatca gcggtttaga ggttatttgc gaaaggggag atttatttct
    12841 gctccgccct tgagtttagc ggggtatgcc agtactataa cgcgtcattt ttcagccgac
    12901 ctttaacacg ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc
    12961 cgtaaggagc aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag
    13021 cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa
    13081 gatccaccgt ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca
    13141 caaagcacac tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga
    13201 aactaccttc cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc
    13261 tgttaccgaa gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga
    13321 tttcgcaaac gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat
    13381 gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc
    13441 atcaggaatt cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg
    13501 ctttcaccgg caggcatggt gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc
    13561 cattactcac agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca
    13621 caaggcaaag aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga
    13681 ttctggagac tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg
    13741 cggaaggcgt tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg
    13801 aaagcggtaa gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc
    13861 tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc
    13921 agtaatcggt cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct
    13981 gcttgataaa gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta
    14041 tgctcgtaac ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga
    14101 attcttcgaa gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagctgc
    14161 taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg
    14221 cgacgaaggt aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc
    14281 agctggcgtt gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac
    14341 tggcgaacac gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa
    14401 cgtagtagct gaataattcg ttattcaacg agacgtaaaa acgccgacca ttggtcggcg
    14461 ttttgctttc tatttttcgt caggcattaa tttcgcaagt agatccattc accattctct
    14521 tgtgtccata agtcaggatg gtttgctggc gttagatgca tacgatcact ccttatgtct
    14581 cggtcctgcg gcactcgctc gaaacctaaa cccgagtaga ttgcggctga atttgataag
    14641 gaacgtagac caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca
    14701 atgatgaatc cgagggcatt agaaacgcct gtaacagtgt gaagttggtc gatataaaga
    14761 tcgattgttg gtagttcaga tgaccagcaa ttaggcattt gggcaggaag ggttacaacg
    14821 tatcctatcg tttggccggt atagtctgga gtactataac caggtcccaa aacaacctcg
    14881 tgtattgtgt atttactgtt gggttcgaag gattcgacat cttgcaataa atatttcagt
    14941 tctgcggtta acctcgcacc cgcgtaagct ttattgtatt tctgggcgtg aatattatct
    15001 aatgtagctt catggttcca tccaaaagaa ttatcaccat tcattgttac tgaatcgcca
    15061 cgggtttaac agacacctca gagtc
//
LOCUS       DAJTRB010000093        14975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTRB010000093 DAJTRB010000000
VERSION     DAJTRB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.093"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..177)
                     /locus_tag="OO330_003222"
     CDS             complement(<1..177)
                     /locus_tag="OO330_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5883992.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHGL"
     gene            complement(593..1534)
                     /gene="fabY"
                     /locus_tag="OO330_003223"
                     /gene_synonym="yiiD"
     CDS             complement(593..1534)
                     /gene="fabY"
                     /locus_tag="OO330_003223"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5883993.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="OO330_003224"
     CDS             complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="OO330_003224"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5883994.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2013..2885)
                     /locus_tag="OO330_003225"
     CDS             complement(2013..2885)
                     /locus_tag="OO330_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5883995.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="OO330_003226"
     CDS             complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="OO330_003226"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5883996.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3577..4362)
                     /locus_tag="OO330_003227"
     CDS             complement(3577..4362)
                     /locus_tag="OO330_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5883997.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="OO330_003228"
     CDS             complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="OO330_003228"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5883998.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5460..6280
                     /locus_tag="OO330_003229"
                     /pseudo
     CDS             5460..6280
                     /locus_tag="OO330_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            6304..7182
                     /gene="yihT"
                     /locus_tag="OO330_003230"
     CDS             6304..7182
                     /gene="yihT"
                     /locus_tag="OO330_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5883999.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7199..8440
                     /gene="yihS"
                     /locus_tag="OO330_003231"
     CDS             7199..8440
                     /gene="yihS"
                     /locus_tag="OO330_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5884000.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPALLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8578..9480
                     /locus_tag="OO330_003232"
     CDS             8578..9480
                     /locus_tag="OO330_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5884001.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9679..11715
                     /gene="yihQ"
                     /locus_tag="OO330_003233"
     CDS             9679..11715
                     /gene="yihQ"
                     /locus_tag="OO330_003233"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5884002.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11761..13135
                     /locus_tag="OO330_003234"
                     /pseudo
     CDS             11761..13135
                     /locus_tag="OO330_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13178..14581
                     /locus_tag="OO330_003235"
     CDS             13178..14581
                     /locus_tag="OO330_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5884003.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14649..>14975
                     /gene="ompL"
                     /locus_tag="OO330_003236"
     CDS             14649..>14975
                     /gene="ompL"
                     /locus_tag="OO330_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5884004.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3677 a         3860 c         3724 g         3714 t
ORIGIN      
        1 caaaccatgc ttcagttcgt taacacaatc gcacagttgc tcctcacctt ccattttaag
       61 gcaaagtgaa aattgattta atagcgtttg cttcggtgat ttattactca ttagttgttt
      121 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
      181 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
      241 agtaaatgaa tgatactgac aatgtatgcc agcccattat ctttgcataa tatttgtgag
      301 ctatagattt taaaaatatt tatgaaaaaa gtatggcacc agagggggtt ttgactatca
      361 aaaatcgctc aaacctgaac taatatgatg agttaagcat tatctgcaat aattgccact
      421 cagctctaca aataatattt aaatgctcct gaacagtgat gtagcaagac acgtatggtg
      481 ccaacgaaga ataaaaattt ataaagttct tattcaatat aaccggaata gtcagggact
      541 gatgtaaatg accggaatga atgagagagg cagcaccgcc cctctccata agctactctt
      601 cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc
      661 cttcaaacac tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg
      721 cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg
      781 catggggttt accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg
      841 ttccgccgag gtggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg
      901 ccagcgagaa taaactcccg gcaaacagcg tatggtgcgg attctgattg ccggtttccg
      961 gcatggtggt gataaatttt tgcccggtat attgttgaat acgcacgccc attttttcac
     1021 taagcgggat atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca
     1081 aaatatcatc cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg
     1141 ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt
     1201 cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg
     1261 tcatcgccat cagcgttcct aaccctttgt cctgcacgtc cgggtgaacg accataaagc
     1321 gaatggacgc ttcattgtcg gcattaatat acaatcggcc taccgccacc agattaccct
     1381 gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt
     1441 ttggttgatg caggggctta cgcaacattt cccagcgaaa ctgatagtaa cgctctaact
     1501 cttcttctgt ttgtggaacc tgaaggtgat acatagcgat actctctctt gtttcccgtg
     1561 acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag
     1621 cgatacctgc atatcggcag cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca
     1681 gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa
     1741 acttgggcgc atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagtac
     1801 actgccgccc gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg
     1861 gtagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac
     1921 acccaataac accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac
     1981 gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca
     2041 gctgcttgtt ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag
     2101 acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg
     2161 taaatgagct gatatgacgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct
     2221 tcgaacagga gtgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg
     2281 gtaggaacaa tgctgtacag caaccagaag gagatccacg acaacagcaa cggaaaaata
     2341 cgcagcacgt tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag
     2401 gaactgatcg ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac
     2461 acggcgaacg agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg
     2521 ctatcgatgg agtacatcaa caataacgcg gtgacgatca gcccgcacgc cccaacggcg
     2581 gtcatcttgt tggaattagc aacaaattgt tcgatatacc gctgaataac atcgccagta
     2641 gcaggcagaa agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg
     2701 ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac
     2761 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt
     2821 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     2881 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     2941 taatgcctag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     3001 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc
     3061 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     3121 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc
     3181 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg
     3241 cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg
     3301 cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     3361 tatgaaaact cttcttaagc gatgccagcg gaatacgcgt taaatcgctc caggctccca
     3421 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     3481 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     3541 ggcaaggtat gaagccaggt taacggtcac aaaaatctac gcgtcttcct ggggaagaat
     3601 gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt
     3661 aaagagcgca gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt
     3721 agtgtgatcg gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt
     3781 agcttcgttt acatcaaact ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt
     3841 taccagataa tcagcacgaa aatcagccac aaaggacgcc gctgaagggc caatgatccc
     3901 gctattatga gagcgcaacg taccgccggg caccatcact tcaaagcgcg ggttgtggta
     3961 aagaatatgc gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa
     4021 cgcccgggca acatgctcaa cagtcgtacc aatggtgata aatattgttg aaccatcagg
     4081 aatatagtct gccaccgctt cggcaatcgc tttttttttc tcggtttgcg aaacctcacg
     4141 ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt
     4201 aatcaggcct tgctcgctta atttacgaat atcccggcgg accgtctgcg tggagacatc
     4261 cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag
     4321 ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac
     4381 catcaccctg gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg
     4441 gatcccggct cgtccaccgg gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg
     4501 gactgactcc gctaaatccc cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac
     4561 atcacccgca cctgtggtat ctaccacatc aactttgaag gccggttgat gctgacgccc
     4621 accattttcc agccagtcgc agcctgcgct accctgggtc acatagacat gaccatttgt
     4681 gagcgtttgt gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag
     4741 acccggttct gaaaaggccg cgtgatcgct taatgccacc agctcactga tatcctgcgg
     4801 cgtaatgtcc ccgtccagaa cggtcatcac acccgcctga cgggccaggg tgaaggcttt
     4861 tttagcgccg tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat
     4921 ttcctccaac cactctgcgt caggcagcag atccgggctg gggtagttaa tgattatccg
     4981 ctcgcctttg gtatccacca tgatggcgga ttgcgaagat ttcgcctggt tataccgttt
     5041 ggtgtaacgg gtgttaacgc cccaggattc cagctctgcc agcaggctgt tgcccgcgtc
     5101 gtcatcacct acgcgaccaa taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc
     5161 tgccgtcgcc gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct
     5221 ctccgtcggt aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc
     5281 aacacgaatc atggtcattc cttaaacatt ttgggatgcg ttcattttaa tttcaccaat
     5341 tgtttaaaaa gtgacggctg tcaatttttt gacgataaat tacaaattcg atcaaaaaca
     5401 gacaagcaaa gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagggtta
     5461 tggcagcaat cgcgtttatc ggtttaggac aaatgggttc gccaatggcg agcaatttat
     5521 tgcagcaagg gcatcaactt cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg
     5581 tagacaaagg tgcgactccc gccgccaacc cggcgcaggc cgctaaagat gccgaattta
     5641 tcattaccat gttgccgaat ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg
     5701 tttgcgaagg cttatctacc gatgcgctgg tcattgatat gtccaccatc catccgctgc
     5761 aaatgccatt accggtactc tgttactgct ggctggcggc accgctgaac aagttgaacg
     5821 tgccacaccg atcctgatgg cgatgggcag tgagttgatc aacgctggcg gtccgggcat
     5881 ggggatccgc gttaagctca tcaacaacta catgagcatc gcgctcaatg cgctttcggc
     5941 agaagccgcc gttttgtgcg aagccctgaa tcttcccttc gatgttgccg tcaaagtgat
     6001 gagcggtacc gccgccggta aaggtcactt caccacttcc tggccaaaca aagtcctcag
     6061 cggagatctt tctcccgcct tcatgatcga tcttgcccat aaggatcttg gcatcgccct
     6121 tgatgtcgcc aaccagctgc atgtgccaat gccgctgggg gccgcctcac gggaggttta
     6181 tagccaggcg cgcgcagcgg gtcacggtcg ccaggactgg tccgccattc tggaacaggt
     6241 ccgtgtcagt gccgggatga ctgccaaagt aaaaatgtaa cgactggata aaggaataaa
     6301 tgaatgaata agtacaccat caacgacatt acgcgcgcat cgggcggttt tgccatgctg
     6361 gcggtcgatc agcgcgaagc catgcgcatg atgtttgccg cggctggggc acccgctccg
     6421 gtagccgata gcgttttaac tgatttcaaa gttaacgctg caaaggccct ctcgccttat
     6481 gcctcggcga ttctggtaga tcaacaattc tgctaccgcc aggtggttga gcaaaacgcg
     6541 attgccaaaa gttgcgccat gattgtcgcc gccgatgagt tcattcctgg caacggtatt
     6601 ccggtcgata gcgtggttat tgaccgcaaa atcaatccgc tacagatcaa acaagacggt
     6661 ggcaaagcct taaaactgct ggtgctgtgg cgtagtgatg aagatgcgca gcaacgtctg
     6721 gatatggtga aagagttcaa cgaactgtgc cactcacacg gtctggtaag catcattgag
     6781 ccagtcgtcc gcccaccgcg tcgtggcgat aaattcgatc gcgaacaagc gatcatcgat
     6841 gccgccaaag agctgggcga cagtggcgct gacctctaca aagttgaaat gccgctttat
     6901 ggcaaaggtc cgcaacaaga gcttctctgt gcttcacaac gtctgaatga ccatatcaat
     6961 atgccatggg tgatcctctc ttccagtgtc gacgaaaaac tgttcccgcg tgccgtacgc
     7021 gtggcaatga cggcgggcgc atcgggattc ctggcaggcc gtgcagtctg ggcatcggtc
     7081 gtcggtttac cagacaacga gctgatgctc cgtgacgttt gcgcaccgaa attacaacaa
     7141 cttggcgata tcgtcgacga aatgatggct aaacgccgct aagatgagga taaaaggaat
     7201 gaaatggttt aacaccctaa gccacaaccg ttggctggaa caggaaaccg accgcatctt
     7261 tgattttggt aaaaattccg tagtgccgac tggttttggc tggttaggca ataaagggca
     7321 aatcaaagaa gagatgggca cccatctgtg gatcacagct cgtatgttgc acgtttattc
     7381 cgttgctgcg gcgatgggtc gacctggcgc ttacgcgttg gttgatcacg gtattaaagc
     7441 catgaacggc gcactgcgtg ataaaaaata tggcggctgg tatgcctgcg tgaatgacga
     7501 gggcgtggtg gatgcctcca aacagggcta tcaacacttc tttgctctgc tgggtgccgc
     7561 cagcgccgtc acaacgggtc acccggaagc gcgcaagctg ctcgattaca ccattgagat
     7621 tatcgagaaa tatttctgga gcgaagaaga gcagatgtgc ctggagtcct gggacgaagc
     7681 cttcagcaaa accgaagagt accgcggcgg caatgccaat atgcacgcgg tggaagcttt
     7741 cttgattgtt tatgacgtca ctcacgacaa aaaatggctg gatcgcgcga ttcgcgtggc
     7801 ttccgtgatt atccacgacg tcgccagaaa taatcattat cgcgttaacg aacacttcga
     7861 tacccagtgg aatccgctgc cggattacaa caaagataac ccggcgcatc gcttccgcgc
     7921 gttcggaggt acaccaggcc actggatcga atggggccgt ttaatgctgc acatccatgc
     7981 tgccctggaa gcccgttgcg aacaaccacc agcattgctg ctagaagatg ccaaaggtct
     8041 gtttaacgcc accgtgcgcg atgcctgggc acccgatgga gcggacggga ttgtttatac
     8101 cgttgactgg gaaggaaaac cggtggtccg cgaacgtgta cgttggccta tcgtcgaagc
     8161 aatgggtacg gcctacgcgc tctacaccgt cactggcgat cgccagtatg aaacctggta
     8221 tcaaacatgg tgggagtact gcattaagta cctgatggac tatgaaaatg gttcctggtg
     8281 gcaggagctg gatgcggaca ataaggtcat caccaaagtc tgggacggca aacaggatat
     8341 ttatcacctg ctgcattgcc tggtgatccc gcgtatcccg ttagcgcccg gcatggctcc
     8401 agcggttgca gcgggtctgc tggatattaa tgcgaaataa ctgatctgaa aatagggtgc
     8461 gggttttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc cggtgcagtt
     8521 tttgggtttg acatcagcct cacgggaggc tgtatgtcgt taattaaggt gccttgtatg
     8581 caaatcacca atatgcattg cagtggacag accgtttcac ttgccgcggg ggattatcac
     8641 gcgaccattg tgaccgtcgg tgctggtctg gctgaattaa cctttcaggg ttgtcattta
     8701 gtcatcccgc acaaacccga agagatgccg ctggcgcatc tgggtaaagt gcttattccg
     8761 tggccgaacc ggatcgccaa cggctgttac cgttatcagg ggcaggaata tcagttaccc
     8821 attaatgaac atagttcaaa agccgctatt cacgggctgc ttgcctggcg agactggcaa
     8881 attagtgaat taaccaccac cagcgttacc ctgacagctt ttctgccgcc cagttacggc
     8941 tatccgttta tgctggcttc acaagttgtt tattcgctca acgcacacac gggtctttcc
     9001 gttgagatcg caagccagaa tatcggcact gtcgcagcgc cttatggcgt gggtattcat
     9061 ccttatttaa cctgcaactt aacatctgtt gatgaatatc tgttccagct tccggcaaac
     9121 caggtttatg ccgtcgatga acacgtaaat ccgacaacgc ttcaccacgt tgatgagctg
     9181 gatttaaatt tcacgcaggc gaaaaaaata gccgcgacaa aaatcgacca cacctttaaa
     9241 accgctaatg atttgtggga aatgaccatt acccatccac agcaggcgct ttcggtaagc
     9301 ctttgttccg atcaaccctg ggtgcaggtt tatagcggtg aaaaattaca gcgtcaggga
     9361 ctggcagttg aacctatgag ttgcccgcct aacgccttta attcaggtat cgacttatta
     9421 ttactggaac caggaaaacc gcaccgcctg ttttttaata tttacggaca gagaaaataa
     9481 cttgtattga ttagcagtca aaatcattga agaacatttc actcgctaaa ggcaacatta
     9541 tgtatcttca atttattaag ttaaaaacag ctcagcttct gtaaagtttt tatatatcag
     9601 ttttatgaga atcattttac ataagcctgt aataacagga aggcaacaat ctcgttctca
     9661 actctatgga gattagttat ggatacgcca cgtccacagt tattagattt tcaatttcat
     9721 cagaataacg acagttttac cctacatttt caacaacgtc ttattttaac ccataacaaa
     9781 gataatcctt gtttatggat tggctcaggt atagcggata tcgatatgtt ccgcggtaat
     9841 ttcagcatta aagataaact acaggagaaa attgcgctta ccgacgccat cgtcagccag
     9901 tcaccggatg gttggttaat tcatttcagc cgtggttctg acattagcgc cacgctgaat
     9961 atctctgccg acgatcaggg ccgtttattg ctggaactac aaaacgacaa ccttaaccac
    10021 aaccgtatct ggctgcgcct tgccgctcaa ccagaggacc atatctacgg ctgcggcgaa
    10081 cagttttcct acttcgatct gcgcggcaaa ccgttcccgc tatggaccag tgaacaaggc
    10141 gttggtcgca acaaacaaac ctatgtcacc tggcaggccg actgcaaaga aaatgcgggc
    10201 ggcgactatt actggacttt cttcccacag cctacgtttg tcagcacgca gaagtattac
    10261 tgccatgttg ataacagttg ctacatgaac ttcgacttta gcgccccgga ataccatgaa
    10321 ctggcgctgt gggaagacaa agctacgcta cgttttgaat gtgctgacac atacatctcc
    10381 ctgctggaaa aattaactgc cctgctggga cgccagccag aactgcccga ctggatttat
    10441 gacggggtaa cgctcggcat tcagggcggg acggaagtgt gccagaagaa actggacacc
    10501 atgcgtaacg cgggcgtgaa ggttaacggc atctgggcgc aggactggtc tgggatccgt
    10561 ataacctctt ttggcaagcg cgtgatgtgg aactggaagt ggaacagcga aaactacccg
    10621 caactggatt cacgcattaa gcagtggtat caggagggcg tgcagttcct ggcctatatc
    10681 aacccgtatg ttgccagcga taaagatctc tgcgaagaag cggcacaaca cggctatcta
    10741 gcaaaagatg cctctggcgg tgactatctg gtggagtttg gcgagtttta cggcggcgtt
    10801 gtcgatctca ctaatccaga agcctacgcc tggttcaagg aagtgatcaa aaagaacatg
    10861 attgaactcg gctgcggcgg ctggatggct gacttcggcg agtatctgcc caccgacacg
    10921 tacctgcata acggcgtcag tgccgaaatt atgcataacg cctggcctgc gctgtgggcg
    10981 aagtgtaact acgaagccct tgaagaaacg ggcaagctcg gcgagatcct cttctttatg
    11041 cgcgccggtt ctaccggtag ccagaaatac tccaccatga tgtgggcggg cgaccagaac
    11101 gtggactgga gtctcgacga tggcctggcg tccgtagtcc cggcggcgct gtcgctggca
    11161 atgaccggac atggtctgca tcacagcgac attggcggtt acaccaccct gtttgagatg
    11221 aagcgcagca aagagctgct gctgcgctgg tgcgatttca gcgccttcac gccgatgatg
    11281 cgcacccacg aaggtaaccg tcctggcgac aactggcagt ttgacggcga tgcggaaact
    11341 atcgcccatt ttgcccgtat gactaccgtt ttcaccaccc tgaaacctta tctgaaagag
    11401 gccgtcgcgc tgaatgcgaa gtccggtctg ccggtcatgc gcccgctgtt cctgcattac
    11461 gaagacgatg cacataccta ttccctgaaa tatcagtacc tgttaggccg cgacattctg
    11521 gtcgctccgg tacacgaaga aggtcgcagc gactggacgc tctatctgcc ggaggataac
    11581 tgggtacatg cctggacggg tgaagcgttc cggggcgggg aagttaccgt taatgcgccc
    11641 atcggcaagc cgccggtctt ttatcgcgcc gatagcgaat gggcggcact gttcgcgtcg
    11701 ttaaacagca tctaatcaca tccgcccgtg gggaaactca tgggcaaagg gagaacatcg
    11761 atgagtcaca tcacaacgga agatccagca actttacgcc tgccctttaa agagaaactc
    11821 tcttacggta ttggcgacct ggcctctaac atcctgctgg atatcggtac gctttatctt
    11881 ttgaagtttt ataccgacgt tctggggctg ccaggcacct atggcggcat tatctttttg
    11941 atttcaaaat tctttactgc gtttaccgat atgggtaccg gcattatgtt ggattcccga
    12001 cgcaagatcg gtccaaaagg taagttccgt ccttttattc tgtatgcgtc attcccggtc
    12061 accttactgg cgatcgccaa ctttgtcggc acaccgtttg atgtcaccgg taaaacggtg
    12121 atggccacta ttctgtttat gctttacgga ctgtttttca gcatgatgaa ctgctcctac
    12181 ggcgcaatgg tcccggcaat caccaaaaac cccaacgaac gcgcctcact ggcggcatgg
    12241 cgtcagggcg gcgctacgct gggcctgctg ctgtgcacgg tgggattcgt gccagttatg
    12301 aatcttatcg aaggtaatca gaaacttggc tatatcttcg ccgccacgct gttttcactg
    12361 ttcggcctgc tgtttatgtg gatctgctac tcaggcgtga aagagcgtta tgtcgaaacc
    12421 caaccaacca atccggcgca aaagcctggc ctgttgcaat ctttccgcgc aattgccggt
    12481 aaccgcccac tgttcattct gtgtattgcc aacctctgca ccttaggggc gtttaacgtc
    12541 aagctcgcca ttcaggtcta ttacacccag tacgtactta acgatcccat cctgttgtcg
    12601 tatatgggat ttttcagcat gggctgtatt ttcatcggcg tgttcctgat gcctggcgca
    12661 gtcagacgtt ttggtaagaa gaaggtctat atcggcggcc tgctgatttg ggtgctgggc
    12721 gatctgctca actatttctt cggcggcggc tcggtcagct ttgtggcgtt ctcctgcctg
    12781 gcgttcttcg gctcagcgcc tgaactgggc gctggtttcc gacaccgtcg agtacggcga
    12841 gtggcgcacc ggcgtgcgtt cggaaggcac ggtctacacc ggcttcacct tctttcgcaa
    12901 agtgtctcag gcgctggctg gtttcttccc cggctggatg ctgacgcaaa ttggctatgt
    12961 gccgaacgtc gcacaggctg accacaccat tgaagggtta cgccagttga tcttcatcta
    13021 cccaagcgca ctggcggtag tcaccattgt ggcgatgggt tgcttctaca gcctgaacga
    13081 gaagatgtat gtccgcattg tggaagagat agaagcccgt aaacgcacgg cgtaataata
    13141 ataaacgacg ccctgcgggg cgttataagg agtgattatg tctgaccata atccactgac
    13201 attaaaactg aatctgcggg aaaaaatcgc ctatggtatg ggcgacgtcg gttcgaattt
    13261 aatgctctgc atcggtactc tgtatctcct caaactttat accgatgaat tagggatgcc
    13321 tgcttactat ggcgggatta tctttctggt cgcgaagttt tttaccgcgt ttaccgatat
    13381 gctcaccgga tttttactcg actcgcggaa aaatattggg cctaaaggca aatttcgtcc
    13441 ctttatttta tatgcagcag ttccggcggc gttaattgca acgctccagt ttatcgccac
    13501 caccttttgt ttgccggtta aaacgacgat tgccaccacg ctgtttatga tgtttggact
    13561 ttcatacagt ctgatgaact gctcgtatgg tgcgatgatc ccggcaatta ccaaaaaccc
    13621 gaatgagcgc gcgcagctcg cggcttaccg ccagggtggt gcaaccatag ggctattgat
    13681 ttgtaccgtg gcgtttattc cgttgcagtc gcttttttct gactcaaccg tcggttatgc
    13741 ctgtgcggca cttatgttct ccattggcgg ctttattttt atgatgctgt gctacagagg
    13801 cgtcaaagag cattatgtgg acacagcgcc aaccggacat aaagccagta ttctcaaatc
    13861 tttttgcgcg atatttcgta atccaccatt gctggtttta tgcattgcta acctgtgtac
    13921 tctggcggca tttaatatca aactggcgat tcaggtctat tacacccagt atgtgctgaa
    13981 tgatattaat ttattgtcgt ggatgggatt cttcagtatg ggatgcatcc tcgtcggcgt
    14041 attactggtg ccagtaactg taaaatgttt tggtaaaaaa caggtttatt tagctggcat
    14101 ggtgctatgg gcggtgggtg atatactgaa ttatttctgg ggaagtaact ctttcacttt
    14161 cgtcatgttc tcttgtgtcg ccttttttgg cacggccttt gtaaacagcc tgaactgggc
    14221 actggtacca gataccgtag attacggtga atggaaaacc ggtattcgtg ccgaaggttc
    14281 tgtttatacc ggttatacct tctttcgtaa aatttctgcc gcacttgctg gcttcttgcc
    14341 aggcattatg ctgacgcaaa ttggttatgt tcccaacatc gcgcaaagcg atgcgacatt
    14401 acagggtttg cgtcagctca tttttatctg gccttgtgca ctggcaatta ttgctgcatt
    14461 gacgatggga ttcttttaca cactcaatga aaaacggttt gcattgatta ttgaggaaat
    14521 caaccaacgc aaaaataaag agatggcgac agaaaaaaaa acggcttccg taacgttata
    14581 aatgtcaata gccgctattt ccatcctggt gggtggcggc ctccctacgt ttaaaaaatg
    14641 gacttattat gaaaaagatt aatgcaataa ttttattatc ttctctgacc tctgcctcgg
    14701 tatttgctgg cgcatacgtc gaaaacaggg aggcatataa tcttgcctcc gatcagggcg
    14761 aggttatgct acgtgtgggt tataacttcg atatgggcgc gggtattatg ttaaccaata
    14821 cctacacctt tcagcgagaa gatgaactaa aacatggcta taatgaaatt gaaggttggt
    14881 atcctttatt caaaccaacc gataaattaa ccatccagcc cggtggctta attaatgata
    14941 agagtatcag ttcgggtggt gcagtgtatc tggac
//
LOCUS       DAJTRB010000094        14910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTRB010000094 DAJTRB010000000
VERSION     DAJTRB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.094"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(90..515)
                     /gene="arsC"
                     /locus_tag="OO330_003237"
     CDS             complement(90..515)
                     /gene="arsC"
                     /locus_tag="OO330_003237"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5884005.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(528..1817)
                     /gene="arsB"
                     /locus_tag="OO330_003238"
     CDS             complement(528..1817)
                     /gene="arsB"
                     /locus_tag="OO330_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5884006.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1871..2224)
                     /gene="arsR"
                     /locus_tag="OO330_003239"
     CDS             complement(1871..2224)
                     /gene="arsR"
                     /locus_tag="OO330_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5884007.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2967..3050
                     /locus_tag="OO330_003240"
     CDS             2967..3050
                     /locus_tag="OO330_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5884008.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3104..4456)
                     /gene="gorA"
                     /locus_tag="OO330_003241"
     CDS             complement(3104..4456)
                     /gene="gorA"
                     /locus_tag="OO330_003241"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5884009.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4528..5370)
                     /gene="rlmJ"
                     /locus_tag="OO330_003242"
     CDS             complement(4528..5370)
                     /gene="rlmJ"
                     /locus_tag="OO330_003242"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5884010.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWQLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5573..7615
                     /gene="prlC"
                     /locus_tag="OO330_003243"
     CDS             5573..7615
                     /gene="prlC"
                     /locus_tag="OO330_003243"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5884011.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7623..8375
                     /gene="rsmJ"
                     /locus_tag="OO330_003244"
     CDS             7623..8375
                     /gene="rsmJ"
                     /locus_tag="OO330_003244"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5884012.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8424..9893)
                     /gene="dtpB"
                     /locus_tag="OO330_003245"
                     /pseudo
     CDS             complement(8424..9893)
                     /gene="dtpB"
                     /locus_tag="OO330_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10211..10645)
                     /gene="uspA"
                     /locus_tag="OO330_003246"
     CDS             complement(10211..10645)
                     /gene="uspA"
                     /locus_tag="OO330_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5884013.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11039..11374
                     /gene="uspB"
                     /locus_tag="OO330_003247"
     CDS             11039..11374
                     /gene="uspB"
                     /locus_tag="OO330_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5884014.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11445..12944)
                     /gene="pitA"
                     /locus_tag="OO330_003248"
     CDS             complement(11445..12944)
                     /gene="pitA"
                     /locus_tag="OO330_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5884015.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGERIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13176..14375
                     /locus_tag="OO330_003249"
     CDS             13176..14375
                     /locus_tag="OO330_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5884016.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3709 a         3788 c         3929 g         3484 t
ORIGIN      
        1 ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa agcgcacttt
       61 accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc ttcatcaacc
      121 actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag aatttccagc
      181 accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat cgggcgatta
      241 atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa tttatcttcc
      301 gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg tacggaaatc
      361 cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc cagataatgg
      421 ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg cgacgtgccg
      481 caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta caaagtgaaa
      541 gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt catgataatc
      601 cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag tacgtgcagc
      661 cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc gcagccaatc
      721 acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc atcaatggac
      781 aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa ggcggtgagg
      841 aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt gagtacgcca
      901 gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata catgccgagc
      961 gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt aatcgcatga
     1021 ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc gctcaccgga
     1081 atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac aacccagcca
     1141 gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag cgccatatcg
     1201 tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt ggcaacaatc
     1261 gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag gccaaagaaa
     1321 tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc ggtatcggca
     1381 atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc taaagccagc
     1441 agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc aaacagggcc
     1501 gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg tccattgccc
     1561 cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag cagcaggctg
     1621 atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca caccaccgga
     1681 atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag cgttgcactc
     1741 cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag gacaaagata
     1801 gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc atatgtgttt
     1861 agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt ctggccaggt
     1921 tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca ataattttcg
     1981 ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct tgcttgcggt
     2041 ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg ggctgcgact
     2101 gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta agcagtaaaa
     2161 cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt aacagaaatg
     2221 acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat aagtcaaaaa
     2281 catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga attttgagtt
     2341 ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt tgcgtcggtt
     2401 ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca catctaacgg
     2461 ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc agctggagaa
     2521 gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc gggtcgctgc
     2581 cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg gtttttttac
     2641 tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct aacgcatcaa
     2701 tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct aattacactg
     2761 tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata aacgctttca
     2821 aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg agtgagattg
     2881 cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag cggatgcatt
     2941 ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt cttggggcgt
     3001 taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga tagcggaaac
     3061 gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc atggtcacga
     3121 actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt ttggttgcgc
     3181 ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag ccaatgccgt
     3241 gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg cacggctggc
     3301 ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact ttcacctgat
     3361 cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca atcggcggat
     3421 ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc ttgttattaa
     3481 acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt tccactgcac
     3541 ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat ttatcgacga
     3601 cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg ttgtcattgg
     3661 caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga ccatcttcca
     3721 gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg gcgttggtat
     3781 gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc atcgggtcga
     3841 agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg aggccgttaa
     3901 tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg cgctctggca
     3961 aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct ggaatatccg
     4021 ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg gtttcgccgt
     4081 taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca tcaacgttat
     4141 ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg gtacggctgg
     4201 cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa tcagggccgt
     4261 acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc ggcacgcagc
     4321 cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat ttctggccgt
     4381 acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg gcgatgtaat
     4441 catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg tagcgcgaga
     4501 cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta cggtggcgtg
     4561 cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat tcatctgctg
     4621 ttccagctgc cacggcgggt taatcacaat catgccggaa gcggtcatgc cacggcgatc
     4681 gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg tcgcttccag
     4741 gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca atgcgtaagt
     4801 gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct gatagtcggt
     4861 tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg gcggcagttt
     4921 ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt ctttctgaaa
     4981 tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca ggctgtcttg
     5041 ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca actggccgct
     5101 acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca aatcgtcctg
     5161 ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat gttcgctacc
     5221 taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt tatctttctc
     5281 tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg cgtggttgcc
     5341 agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa gtaaaaattg
     5401 gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga ggccgccgcc
     5461 gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct acacacatta
     5521 tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc taatgacgaa
     5581 tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg aacatgtcgt
     5641 tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg tagtagcgca
     5701 aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg acgatgtttt
     5761 ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc cggaactgcg
     5821 tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg tagggcaaca
     5881 tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg ccacgctgaa
     5941 cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct ctggcatagg
     6001 tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt ctgaactggg
     6061 caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac tcgttaccga
     6121 cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag cccaggccga
     6181 agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc tgccggtaat
     6241 gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca gcacccgcgc
     6301 ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg aagagatcct
     6361 cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct ttaaatccct
     6421 tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg atctggcaaa
     6481 acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg ctaaagccga
     6541 atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa aacagaaaca
     6601 gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa acaaagcggt
     6661 taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag agcgtaaaga
     6721 tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa ataacgaact
     6781 gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg gggcgtggat
     6841 ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac cggtcgcgta
     6901 tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca ctcacgacga
     6961 agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga cccgcatcga
     7021 aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac tgccgagtca
     7081 gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg gtcactatga
     7141 aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga agaactacca
     7201 ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc gccttcatgc
     7261 cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa tcaagaaact
     7321 ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca gccatatttt
     7381 cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac tggcggcaga
     7441 tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc agtcgttcct
     7501 cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca aacgcttccg
     7561 tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg gctgatcatt
     7621 cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta tctgttctgg
     7681 cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg ttaacgccgg
     7741 aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt gttgattttg
     7801 ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag gcggtggcga
     7861 aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc gcagggctgg
     7921 ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg gagcgtaatc
     7981 cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat gcggaaatcg
     8041 gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg gcgctaaccg
     8101 atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat aagcagaaaa
     8161 gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg gatcttgatg
     8221 ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg gtaaagcgcc
     8281 cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg actaaagggc
     8341 atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta gcgataacgc
     8401 taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat acgtttcagc
     8461 cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc aatcttacca
     8521 aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc cggcaccgcg
     8581 gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag gaaccacatc
     8641 ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc caggccaagg
     8701 gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat aaaccatggc
     8761 gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt caaaaagccc
     8821 agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc tttgcctttg
     8881 ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac ccagaacggg
     8941 ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg atgcacgttg
     9001 ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta aaacaccacc
     9061 gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt atccagcttg
     9121 aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag caccagattg
     9181 gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac gctgccaagt
     9241 aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga accaatgtct
     9301 ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc cgccccgcac
     9361 aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag cgataacgct
     9421 atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc aagccgcgga
     9481 tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa caggccgtta
     9541 ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag cgacatgccg
     9601 gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg tttggtcccc
     9661 aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag tgcagcaaaa
     9721 gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac gaagaaaacc
     9781 gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac aaaaaagatc
     9841 atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt aatatttatt
     9901 aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc gaaaaagttt
     9961 acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact attacaacag
    10021 aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat tttgtattgg
    10081 caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac gctgcatcag
    10141 gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg aacacgtcgt
    10201 ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca acgtgaacgg
    10261 tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg tggtgaccac
    10321 aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg cctaggtcgc
    10381 cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc tcggtcagcg
    10441 catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca tcaataagcc
    10501 cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc gcattgtagg
    10561 ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag agatcgactg
    10621 cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt cgatgactgg
    10681 ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg tgcgcggggc
    10741 taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga cccggatgat
    10801 ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa tattatattg
    10861 atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct atagagccgc
    10921 tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact gattggcagc
    10981 gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg gaggaggtat
    11041 gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta acatggcgcg
    11101 ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc cattgctcta
    11161 tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca aacaggtgcg
    11221 tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg agtttattcg
    11281 ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg gtctggtggt
    11341 ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat aacgtataaa
    11401 aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag gaactgcaag
    11461 gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa cacccaggcc
    11521 atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac catcgtcccc
    11581 gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata actcgccagg
    11641 ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt catgcctttc
    11701 ttaccgattc tctcaccgat agtcgttgcc acacgacgcc agccaatcat cgtaccgata
    11761 cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat ggtgctaagc
    11821 atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc aggcatcttc
    11881 accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg gctacgttga
    11941 tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt caggcggttg
    12001 agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt tgcgccagct
    12061 tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc aggatgctgc
    12121 tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc gtagccagtg
    12181 gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa cataaccaga
    12241 ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat agcggaaagg
    12301 atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt ttcacgctcc
    12361 gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg acgcagcaag
    12421 aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag agaaccgaaa
    12481 atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt catcaacgca
    12541 ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga tgcaggtaaa
    12601 ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga gaacaccatg
    12661 gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat atgcacaatg
    12721 gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac cgccgccata
    12781 accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac ggcgttggct
    12841 gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc aagcaataat
    12901 aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg ttacgccatt
    12961 ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa agtgaaatat
    13021 agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta tcttatataa
    13081 ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga ggaatcttta
    13141 caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat gccattatta
    13201 taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta ggacgccggg
    13261 ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct ggcggtgggc
    13321 gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag aatccgcatt
    13381 tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg gtcaataaac
    13441 acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac tccgcgcagc
    13501 agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc agattgcgta
    13561 gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg aacggcatga
    13621 ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg gggctgggcg
    13681 catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg ccgacccgcg
    13741 cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag gtgctggcgg
    13801 gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt gagaacttac
    13861 tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc tactggcaac
    13921 cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc ttcctgaatg
    13981 agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt catctaccga
    14041 agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg ctaaaacagc
    14101 tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc gtacaaccca
    14161 acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac accaacgaac
    14221 tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc ggcgaagtga
    14281 tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg agtgcatggg
    14341 cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc gtcagccgtt
    14401 tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc tggtgttaac
    14461 tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata gtgtatttat
    14521 tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag gaaattattt
    14581 gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc tctggattga
    14641 atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta tcaggtggtg
    14701 tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat atggctattc
    14761 ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct tgccatgaga
    14821 gtcgtgatat tattatgcat catttttagg taatgctgcc aacttactga tttagtgtat
    14881 gatggtgatt ttaaggtgct tgcgtggctt
//
LOCUS       DAJTRB010000095        14670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTRB010000095 DAJTRB010000000
VERSION     DAJTRB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.095"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(237..599)
                     /locus_tag="OO330_003250"
     CDS             complement(237..599)
                     /locus_tag="OO330_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR5884017.1"
                     /translation="MRDIQMVLERWGGWAASDNSGVDYSPIAAGFKGLLPQTGKNRLS
                     CTDADGLIIEGCLARLKKKKPYEHSLLVAHYLYGLSKRKIAKEKKKDEKLIRIEIKMA
                     EGFIEGCLSMLDIRLEMD"
     gene            complement(617..1606)
                     /locus_tag="OO330_003251"
     CDS             complement(617..1606)
                     /locus_tag="OO330_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5884018.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPDSFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALATGVLA"
     gene            complement(1614..2429)
                     /locus_tag="OO330_003252"
     CDS             complement(1614..2429)
                     /locus_tag="OO330_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5884019.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYS
                     RTSINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(2592..2987)
                     /locus_tag="OO330_003253"
                     /pseudo
     CDS             complement(2592..2987)
                     /locus_tag="OO330_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            complement(2984..3310)
                     /locus_tag="OO330_003254"
     CDS             complement(2984..3310)
                     /locus_tag="OO330_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5884020.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTVVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(3310..3804)
                     /locus_tag="OO330_003255"
     CDS             complement(3310..3804)
                     /locus_tag="OO330_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5884021.1"
                     /translation="MIMSLLNEVQKYIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEAEPQPVRETRPVRNFYVGTNDPREILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSSDEWYLSGN
                     YVGA"
     gene            complement(3801..4742)
                     /locus_tag="OO330_003256"
     CDS             complement(3801..4742)
                     /locus_tag="OO330_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5884022.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(4732..4911)
                     /locus_tag="OO330_003257"
     CDS             complement(4732..4911)
                     /locus_tag="OO330_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5884023.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(5087..5638)
                     /locus_tag="OO330_003258"
     CDS             complement(5087..5638)
                     /locus_tag="OO330_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884024.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(5682..5882)
                     /locus_tag="OO330_003259"
     CDS             complement(5682..5882)
                     /locus_tag="OO330_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5884025.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            5973..6647
                     /locus_tag="OO330_003260"
     CDS             5973..6647
                     /locus_tag="OO330_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HCR5884026.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            6882..7088
                     /locus_tag="OO330_003261"
     CDS             6882..7088
                     /locus_tag="OO330_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884027.1"
                     /translation="MHKTEPKIVAPGYTNEEIYEWMAKKLAAINQLREVLSYRQETID
                     SLKKLDQEITVLSQDVTLDIVQTN"
     gene            complement(7060..7494)
                     /locus_tag="OO330_003262"
     CDS             complement(7060..7494)
                     /locus_tag="OO330_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884028.1"
                     /translation="MSGNIYTLYKSHCENVGKYRGIEISGVVSSVEISKVESRATLLT
                     LLDLVLHEHRKKFGTPYNQLNGKKALVHLILMKHHWMPKQINEMKFDELLLSIQDELT
                     LDKISVTAQKFLDYRDWRSQIHHFDDFDENEWDPNLSAQYLK"
     gene            7638..7948
                     /locus_tag="OO330_003263"
                     /pseudo
     CDS             7638..7948
                     /locus_tag="OO330_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7945..8325
                     /locus_tag="OO330_003264"
     CDS             7945..8325
                     /locus_tag="OO330_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884029.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGR"
     gene            8391..9215
                     /locus_tag="OO330_003265"
     CDS             8391..9215
                     /locus_tag="OO330_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HCR5884030.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            9343..9879
                     /gene="yfdR"
                     /locus_tag="OO330_003266"
     CDS             9343..9879
                     /gene="yfdR"
                     /locus_tag="OO330_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5884031.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            9870..10232
                     /locus_tag="OO330_003267"
     CDS             9870..10232
                     /locus_tag="OO330_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884032.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            10232..10537
                     /locus_tag="OO330_003268"
     CDS             10232..10537
                     /locus_tag="OO330_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884033.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            10764..11927
                     /locus_tag="OO330_003269"
     CDS             10764..11927
                     /locus_tag="OO330_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5884034.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(11942..12017)
                     /locus_tag="OO330_003270"
     tRNA            complement(11942..12017)
                     /locus_tag="OO330_003270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11982..11984),aa:Thr,seq:cgt)
     gene            complement(12132..13385)
                     /gene="proA"
                     /locus_tag="OO330_003271"
     CDS             complement(12132..13385)
                     /gene="proA"
                     /locus_tag="OO330_003271"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5884035.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(13397..14500)
                     /gene="proB"
                     /locus_tag="OO330_003272"
     CDS             complement(13397..14500)
                     /gene="proB"
                     /locus_tag="OO330_003272"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5884036.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         3796 a         3723 c         3480 g         3671 t
ORIGIN      
        1 gcaattggta tgttcgctat ttccagagag tcgatagttt atctatattt tatatctgct
       61 tgtggaggag gcattgctgc tgcaatgtat tcttttttct ttatcagctc aataaacagc
      121 gaaatacaga gatttaaaag catcttatta cacagagctg aagcgaataa aaaaacgaaa
      181 gaatttatag actcattaaa caaaaaggcg gattgaaatc cgcctttttg ttttcttcaa
      241 tccatttcta atctaatatc caacattgaa agacaacctt cgataaatcc ttcagccatt
      301 ttaatctcaa tgcgtataag tttctcgtcc tttttctttt ctttagcaat ttttctctta
      361 gaaagaccat acaaataatg tgctactagt aacgaatgct cataaggttt tttcttcttc
      421 agccgagcaa ggcacccctc aatgattaat ccgtcggcat cagtacatga gagtctgttt
      481 ttacctgttt gtggtaaaag ccctttaaat cctgctgcga ttggagaata gtcaacgcct
      541 gaattatcac tagcagccca tcccccccaa cgctcaagaa ccatctgaat atcacgcatc
      601 aaatttctcc acaaaatcag gccagcacgc cagttgccag cgcacgatcg ataaaacgaa
      661 atatcagctc cagctgagag ccatacttct cttcaaatgc cacggtatcc gcatgcagct
      721 cgtcgtgatg ctttctgcac aaaggcaaca caaagaggtc atgcgctttt gtacccattc
      781 ccccctgacc gtggcctatc aggtggtggg gatcatcagc aggttttcca caacatgcgc
      841 acggctgcgt cttaacccaa cgcgtgtact tttcgttaac ccagcggcga cgttttgggc
      901 gtaacataaa agagtccggc gactccggat ccactttcag cgccagcacc ttttttgcct
      961 tatcctggat gatgctggtg gcaggaaccg aaggcacaag gtcactttcc cgggtgacag
     1021 acggcacaac aggctttggt aatctcagtg ccttacgggc tgcactttcc ggtaaggcat
     1081 ccgccaggtc attacgaacc agccaccagc acagttccgg cattgtcaca acgtgactat
     1141 catcaaaacc gagatcccga cgcacaacag acaacaccca gcgggcacag ttatccgttg
     1201 ccattgattc cagccgttcc gtgaactgat cgcgcagctg gttatcgcag tgccagcaca
     1261 gacggattgc gcccggagcg tgtcgcattg tggtcatgtt ctcgctgtgc cagtcggaat
     1321 gaggccactg gcagcctttt tcacgaagta accagctttc aagacattcc acgccaccag
     1381 cacgacggat cactgcctca ttgcggaaca cggcccgaac ggcaggatca tccgccagcg
     1441 gttgtgatgc cgccggaacg gcaccgctgg cgaaagatga ataacgttcc ggctcaggct
     1501 ccagcaggac acgcccctgc ataaacaggg gcatcagctc tgaaccaggt ctgaacaata
     1561 cgatccccat acgcggggca atttcagggg tcagtagtgc tctcacagtc acctcaatga
     1621 acggtatcga gcagctttaa cagctcaggg aatcgggatt cgaagaaatg cggctgcgtc
     1681 tcgcgcggat ttgcgggact ggtgatgttc ttgccgaaca tgcagccttt cgctgtcagc
     1741 gaccagaatt ttttgatgtt gttaatcgag gtacggctgt atcgttcgcg ctgctcgacg
     1801 atccccagct tcaccatctg gtgatatgcc tgattagccg tcaggcggat accatactgc
     1861 ttcagcagtg cactcagcga cagcgtaggg cggcttgaac catctggcgc atcagcaggt
     1921 gcatcaatgg catagatcgg cataagttca ggaagaccag ctacctttga taatttctgg
     1981 tatgcaccaa gtttcgagga gtttgacaga tttagagtct ttgctgctga ttcaagcaga
     2041 atgaccccgg atttaatttt gtcggatgtg gtttcttctg gtgatgaatt atgaagcgca
     2101 tcaaaagtac gtatcacttt taagctgaat gccgggctga tccacattgc atatgcatag
     2161 accagctctt tacagacata cgtcccacca ttgcgcccct gaatggtgat gacaggaata
     2221 ctacgggaat ctcccgtagt ttcttcttcc aataattcca caagagcctt cgtttcagga
     2281 cgacgcataa actcgtgaac ttccagcgaa cgggaggagc gattctcacc agcggcaaga
     2341 agagcagctt tctgaaggtc gttaagacag tagttagatt cgaagtactg gcgcacagaa
     2401 acgccatcaa ttacaagcaa ctgattcatt ggtttctcca caaattttta tccacgagcg
     2461 ggactgcact cccttttcgt tgatgcagga tgaacttact gcgattttta atagttatca
     2521 aggatatact gttcataaat acagtatctt taacgaggta atacccaaat ttagggtgtt
     2581 gctcaattcc gttaccgagt tgctaatttg caactcgctt tttcgtactt actgatagtg
     2641 atctcgacct tcccctccgg gataaccggt ccccactcca ccagcattct tttcacctga
     2701 ctgtcgtctt cccacacacc cgcgtgggtc agggcgtcaa acagcgcctt gttatagttg
     2761 tccagatcgc ggatccggtt atccggagga aacaaaacga tctccactga agcaggtgcc
     2821 gacgttggtt tcggcagacg acgtaactgc tcaactattg ctgcgcacgc cgcgctctgg
     2881 aattttcacc ccgccgcgct tatcaggctc ttaccagcaa acgccccttt gttggggtgt
     2941 cgccagtacg tgttcacgct gggcggaaaa ggaaggatca gcttcatact ttcaggcccc
     3001 tctcatgtaa ccagtgggct gcacgcagcc tggcgttttc ctcaccggca agcagtgagc
     3061 ggataatccc gaccgcctcg ctgtcgtcgt ccttcaccac ggtatgaagc gtgatccccc
     3121 gggccacacc acgctttatc gtgatgacgc cttttttctc cagtgcgcga agatgctcca
     3181 ccgctgcatt caccgaacgg tatcccagca tggttgccac ctcctgattg gttggcggga
     3241 agccacgttc tttctgataa gaaatcagca tatccagcac ctgctgctgg cattgagtta
     3301 acgtcgtcat tacgccccca cgtaattccc tgacagatac cactcatcac tcgatacagc
     3361 gcgcttgctg cttttccgta aacactgctc acgacgcgcc agaaaattgt ttcgttctgg
     3421 ctgggagtgg ctttcacgga atgccgccat ccacaccgtt gcagcacgac ggtataagcc
     3481 cctggactcc agttcttcag cctggcgggt caggcacaaa atctcccgcg ggtcgttagt
     3541 gccgacatag aaattgcgca caggtctggt ttcacgaact ggttgcggtt ccgcctcctg
     3601 cgctctctca gtcaggcgcg ggaaatgtct gcgtgtatcc ccttcacaac ggtgagccac
     3661 acgcccactc tgacgtaact tgcttgctga ctgcagaacg cgctgccgtg agtaacctgc
     3721 aaaagcatcc gcaatgtctc cggaagtaca ccccggatgg gcttcaatgt atttctgaac
     3781 ttcattcaaa agactcatga tcaccccctg aatcctgccg ggatctggct gtagtccacg
     3841 ttgtcgtaac tggctttgaa gtacgggtct tcgcgttttt ctgtgtacgt gctgacggac
     3901 ggcgataagc gcagggaaag ctcatcccat ttttcccgca gcttcgacgg gctgagcacg
     3961 ttacggcacc agaacggatc gcggctgacg cggctgtaca tctcgcagat ttgtttgtga
     4021 gtacgaccat cctgcacaca catcaggcga atttcgtttg cccaggctgt ccagttcggt
     4081 tctttgggac gaaccacctc gccgtcacat tcggcggcct gctcgtacag ggcgatgatt
     4141 tttttccaga gccactgtgc gcaggtcaaa tcatcctgcg ttccccactg gcgcttttta
     4201 gggctgaata caaccgcatc aggatggcga gttaaaaact cctgttcagc cgtctgcgtg
     4261 tccggttgcg aagcgtccgg acgagaagtt tttttatctg acggatcatg ttttgatttt
     4321 actgacggat ccccgccaga ttctgacggg tgaaaacccg cttttttgcc agatttcgac
     4381 gcatcaaatt ttgacgggtc agattttgat gcgtcagatt ttgacgggtc agaatctgac
     4441 agttgagaaa atgccgctgc ctgaagcttc gcaacgttaa gctgataaac attcgacgca
     4501 ttgcggttac cctggcgacg cgccttacgc gttaaccagc cttctgcttc cagccgtgcg
     4561 atagccgttc tgacggtact catccccgcg ccaatctgac gggcaatggt ttcaattgat
     4621 ggccagcaca caccttcgtc attactgaaa tcagccaggc gggccataat tgccacgctg
     4681 gataatttca tgcctgacgc tgcgcaacca tcccatacat agccggttaa tttagtgctc
     4741 atgaccgacc tctatttccc tgaatttacg acgaaactgt tcgagcgggc tgaagcactc
     4801 atgctcatag ccttcacgga ggtagataac ccgttgtgtt tccggctccc aacgaatgac
     4861 tctgacgggc actccgtagt gatctttgaa ccagcgatta acttgtcgca aaggactgtc
     4921 tccttctgcc ggttgaaatc ccccacagcc cactctgcaa agctgtgggt tacaatttcc
     4981 ctgtcacctg gtacattcac tgcatagcaa tactccacct tcgcttttcc acccggtaca
     5041 ggaagcgcaa tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt
     5101 ctccacagtc acgacacgcc acggcgcccg gagctgcaca ctcgcgggcg tcattacttt
     5161 ctgaaatgca aaaaattttg tagaccagtg ctgcatgctc ctgcagcttc gaaattgaga
     5221 ggtacagctc gtcgttaatt gctgtcttct catgcggttc cactacaccg tcttcgattg
     5281 ctgaacgaat ctgttttgaa taactgccga tctgttcaat gacttccagc agacgctggt
     5341 taatatcggc gttgtccaca tcctcgacgt caggaagaga cacaaagacg ccatttgcag
     5401 actgcgccac agcgtcagca atgaagtgag ttccaccagc acgttgcaaa atcattgccc
     5461 atcccagcgg gaaaatctga tcgccatcgg cacgaaggcg gttaaataat gcgttctctg
     5521 ttacatccag ccagtcagct gcttcagcgt aaccacccgg caactttgcg atagtttttc
     5581 tgacagcttt cacgtaccac tcaggctgtt tttctacttt ccagtgatgc ttacccacgg
     5641 ttagcctcat cgttctgtgg ttaaaaattg aaagtgttct gctaatcttt cggatagata
     5701 tccggtctta agtcagattt cgtaattgca cctgacgtgc attgctcaag ttttttagcc
     5761 agcacaaaac tggctttttt atagccattg aaaaccagcc gtaagtagcc aggtgttgag
     5821 ccaacttttc cggccaactc gccctgctgt tctttggtta aagagtccca atacgctttc
     5881 atacaatatg tacctccggt gtacatatta catgattaaa atgaaccttc aagatacttg
     5941 taccttaacg gtacaagggt tttaatttcg ttatgaaaac aatccatgac atccggcggt
     6001 ctaacgccag aaaactgaga gatggtgttg gcgggaattc ttcctttgcc actatgattg
     6061 atcgcgagcc aacccagacc agcaggttta tgggagatgg tgctactaaa aatatcggtg
     6121 acagcatggc acgacacatc gaaaaatgtt tcgacctgcc tgttggatgg ctcgatcaag
     6181 aacaccagac aacgaacatc acaaaaaaac ctgatgtttc aatcactaat aaacaaatca
     6241 cattagtccc tgtcatatca tgggtacagg ccggagcatg gaaagaagtt ggatattctg
     6301 aggttgattt gagcacagca gaaacgtatc cctgccctgt accctgtggg gaaatgactt
     6361 atatcttgcg ggtgataggt gattcaatga ttgatgagta ccgcccggga gacatgattt
     6421 ttgtcgatcc tgaagtacca gcctgccacg gtgacgacgt tattgcattg atgcacgata
     6481 caggtgaaac caccttcaaa aggttgatag aagatgggac acagcgttat ctcaaagcgt
     6541 taaacccaaa ctggcctgag ccttacatta agatcaacgg taattgctct ataattggaa
     6601 ctgtgatttt ctcaggaaaa ccaagaagat acaaaatcaa agcctaatca atgtttatga
     6661 acctgcttcg gcaggttttt ttatacttga caatgtaccc atgagataca taatgtaccc
     6721 aagagaaaca acgaacaggc aggacgccca cgaagtagcc gcctggggca tataaagtct
     6781 agggtgattc gttagcaaca aaaaagcgcc ctacaggacg cttagctctt taacaatctg
     6841 gtccccatca acaagtaact gataacttga ggaggtgtga aatgcacaaa acagaaccca
     6901 aaatcgtcgc gcccggatac acaaatgagg aaatttacga gtggatggca aagaagctgg
     6961 cagctataaa ccagcttcgt gaagtgctgt cttatcgaca ggaaacaata gactccttaa
     7021 aaaaactgga tcaggaaatc acggttttat cacaggatgt tactttagat attgtgcaga
     7081 caaattagga tcccattcat tttcgtcaaa atcatcaaag tgatgaattt gtgatctcca
     7141 gtctcgataa tctaaaaatt tctgggcggt tacgcttatt ttatcaagtg tgagttcatc
     7201 ctgaattgaa agaagaagtt catcaaattt catctcatta atctgttttg gcatccagtg
     7261 atgcttcatc agaataaggt gaaccagagc ctttttccca ttcaactgat tatagggagt
     7321 gccgaatttc ttccggtgct catgtaagac aaggtccaga agagtaagta atgttgccct
     7381 tgattccact ttgcttattt cgactgatga cactacccca ctgatttcaa tgccccgata
     7441 ctttccaaca ttttcacagt gggatttgta cagcgtgtag atattaccgg acatttcttt
     7501 tccttttgcg ttgttgggga taaccagatt aaccgaatcc ttgttgttgg gaaataacca
     7561 ggtccacctc gcctgatgtg gctaaaagca ggcacataac agctaagtat ttttaaccag
     7621 agagaatcct tagcgttgtg gtgaatgcgg ctcagcgcac gcgggttaag gttgaggctg
     7681 acagtcgacc ttctgtggat acccacccgt ctggtgtgca accttcgcca ggcaccggga
     7741 ggcacccggc accacaactt tatgctgtgt gtagtcctgg aggtaccagt ttgtaccctt
     7801 gcttccggct ggtaccgtcc tttttacaaa acagagaaga gcatcaccgg acgacgggct
     7861 cataacccaa tccatccggg cggctgccac cgcaggtgtt cttctctgtt ttgtggagaa
     7921 actaatcggc cttgcagggt cgatatgatg aggagcagca aaatggctag cgaacgcagt
     7981 actgatgtgc aggcatttat cggggagctg gacggcggcg tatttgaaac caaaatcggc
     8041 gcagttctca gtgaagtcgc ttccggtgtg atgaacacga aaaccaaagg taaggtctca
     8101 ctcaacctgg aaatcgaacc atttgatgag aaccgtgtga aaatcaaaca caaactctca
     8161 tatgttcgcc cgactaaccg cgggaaaatt tccgaagaag acaccaccga aacgccgatg
     8221 tatgtcaatc gcggtggtcg cctgactatt ctgcaggaag accagggaca gttactgact
     8281 cttgccggtg aacctgacgg aaaactccgc gcagcaggtc gttaatatcg tttttaatta
     8341 actgattatt tatctcatca ctgaatatct ttatatagtg aggacttatt atgtctcaga
     8401 acttagacgc aaccgcaatt aatcaaatcc atgcccttat ttctgctcag ggtgttaatg
     8461 aaattatcag taagattggt gccgatgctg tggcattgcc tgagaatttc cgcattcatg
     8521 atctggaaaa atttaattta aatcgcttcc gtttccgtgg tgcgctttcc actgccagca
     8581 tcgatgactt tacccgttat tctaaagatc ttgcagatga aggcacccgc tgctttatcg
     8641 atgctgataa tatgcgtgcc gtcagtgtgc ttaacctggg tactattgat gaaccaggtc
     8701 acgcagataa caccgccaca ctcaaactga aaaagacagc accgttctct gctctgttgt
     8761 ctgttaacgg cgagcgtaac tcccagaagt cactggcaga atggattgaa gactgggccg
     8821 actatcttgt gggctttgat gctaatggtg acgctattca ggcaacaaaa gcggctgcgg
     8881 ctgtccgtaa aatcacgatt gaagcaaacc agaccgctga ttttgaagat aatgacttca
     8941 gcggcaaacg ctccctgatg gagtctgtcg aagcgaagac caaagacatt atgccagtgg
     9001 catttgaatt taaatgcgtt ccgtttgaag gtctgaaaga acgtccgttt aaattacgcc
     9061 tcagtattat cactggcgat cgtcctgtac tggttctgcg cattattcag ctggaggcgg
     9121 tgcaggaaga aatggctaac gaatttcgtg atctgcttgt tgagaaattc aaggacagca
     9181 aagtagaaac ctttattggt actttcaccg cctgatttca ttactgcaaa tgcccctgcg
     9241 ggggcattta tggaaacgta atttactcaa taatcgccgg atggtgaggg attcttttta
     9301 ccagaattca gcgcggtgca gcgcatatac gtggagaaca aaatgtcatt tattaaaact
     9361 ttttccggga agcattttta ttatgacagg ataaataaag acgacatcga tattaacgat
     9421 atcgcggttt ccctttcaaa tatctgtcgc tttgccggtc atctttcgca cttctacagc
     9481 gtcgcccaac atgcggttct ttgcagccag ttggtgccgc aggaatttgc ttttgaagcg
     9541 ttaatgcatg atgcaacaga agcgtattgc caggacattc ccgcaccact gaaacgcctt
     9601 cttcctgact ataaacaaat ggaagaaaaa atagacgccg taatccgtga gaaatacggg
     9661 ttacccccag ttatgagtac gcccgtgaaa tatgccgatc tcatcatgct ggcaaccgaa
     9721 cgccgcgatc tcgggcttga tgatggctct ttctggcctg tactggaagg tatcccggca
     9781 acagagatgt tcaacgtgat tccactggca ccgggccatg cctacgggat gtttatggaa
     9841 cgctttaacg agttatcgga gttacgcaaa tgcgcatgaa tgttttcgaa atggaagggt
     9901 ttcttcgtgg gagatgtgta ccgcgagatc tgaaagtaaa tgaaacagat gctgaatacc
     9961 tggtgcgtaa attcgatgcg cttgaagcta aatgtgcagc acaggaaaac aaagtaatac
    10021 cagtgtcaac tgaactgcca ccagcaaatg aaagtgtttt gttattcgat gctaacggag
    10081 aaggctggct aattggctgg cgttctctct ggtacacctg gggacaaaaa gaaaccggag
    10141 aatggcagtg gacatttcag gtcggggacc ttgaaaacgt caatatcact cactgggcag
    10201 taatgccaaa agcaccggag gctggagcat aatgaccact tttaccgaca aagaactgat
    10261 taaagaaatt aaagagcgta tcagcagcct tgacgtgcga gacgatattg agcgccgtgc
    10321 ttatgaaatc gcactcctat ctctggaagt agaaccagat gaacgcgaag cttatgaatt
    10381 attcatggaa aagcgtttcg gtgacttagt agatcgtcgg agagcaaaaa acggcgataa
    10441 cgaatacatg gcatgggata tgactctcgg ttggatcgtc tggcagcaac gagctggtat
    10501 ccatttttca acaatgtcac agcaagaggt gaaataatgg agccatacag cctcacactc
    10561 gatgaggcct gtcattttct caagatatcc agaccgactg ccattaactg gatacgcaca
    10621 gggcgtcttc aggcaacacg caaagatccc actaagaata aatctcctta cctcacaaca
    10681 cgacaagcct gcattgcggc tcttcagtct ccgctgcata ctgtccaggt gagcgcgggt
    10741 gatggcataa cagaggaaag aaaatgtcac tcttccgcag aggtgaaata tggtacgcca
    10801 gtttcacatt gccgaacggt aaaagattta aacagtctct tggaacaaag gacaaaaggc
    10861 aggcgacaga actccatgac aagctaaagg ctgaagcatg gcgggtcagc aaacttggtg
    10921 aaatacctga tataacgttc gaggaagcgt gtgtcaggtg gcttgaagag aaagcacata
    10981 aaaaatcact ggacgatgac aaaagccgga tcggattctg gcttcaacat ttcgcaggaa
    11041 tgcaactaag agacattact gaatcaaaaa tttattcagc aatgcagaaa atgacgaacc
    11101 ggcgtcatga ggaaaactgg aaactcaggg cagaagcatg cagaaaaaaa gggaaacctg
    11161 ttccagaata cacgccaaaa ccagcgtccg ttgcaacgaa ggctacgcat ctttcattta
    11221 taaaggccct actaagagcc gcagagcgtg aatggaaaat gctggataag gcaccaatta
    11281 ttaaagtgcc tcaaccaaag aataaacgga tccgctggct ggagccccat gaagcacaaa
    11341 ggctgattga tgaatgtccg gagccattaa agtctgttgt tgaatttgca ctggcaacag
    11401 gcttaagacg ctcgaacatc atcaaccttg aatggcaaca aatagatatg cagcgccggg
    11461 tggcatggat aaacccggaa gagagtaaat caaaccgcgc aattggcgtt gcgctgaatg
    11521 atactgcatg tcgcgtattg aaaaaacaaa tcgggaatca tcaccgttgg gtatttgtgt
    11581 acaaggaaag ctgtaccaaa ccagacggaa cgaaagcgcc aacagtaagg aagatgcggt
    11641 atgacgcaaa cacagcctgg aaagcggcgc tgagacgggc tggtattgat gatttcagat
    11701 ttcacgactt gagacacacc tgggcaagtt ggctggttca agccggagtc ccgttgtcag
    11761 ttttacagga aatgggaggc tgggagtcta tcgaaatggt tcgtcgatat gctcaccttg
    11821 cacctaatca ccttaccgaa cacgcacggc aaatagactc gatcctgaac ccatcggtcc
    11881 caaatttgtc ccagtcaaaa aataaggaag gtactaatga tgtgtaactt attgatttta
    11941 atggtgccga taataggagt cgaacctacg accttcgcat tacgaatgcg ctgctctacc
    12001 aactgagcta tatcggccct gaaaggacat gttcacgaac gtgaatcacg gtggacaagg
    12061 ttaaaactaa ccgggcgatg cgtcaatggc cttgtgaatc aaatggctac ttttgcatca
    12121 cccggtttta tttacgcacg aatggtgtaa tcaccaatgc cgatccactt gtaagtggtc
    12181 agtgcttcca gccccattgg gccacgcgcg tggagttttt gtgtgcttac cgccacttcc
    12241 gcacccagac caaactggcc gccgtcggta aaacgcgtag aggcgttaac gtaaacagcg
    12301 gacgaatcca cttcgttaac aaaacgctgg gcgttgcgca tatcgcgggt caggatcgca
    12361 tcggagtgtt gtgtgccgtg ttcacgaata tgggcgatgg catcgtcaag atcgctgacg
    12421 attttgacgt tcaaatctaa tgacagaaac tcatcgtcat actcttcggc tttaaccgcc
    12481 accaccttcg cagggcctgc ctgcaactgt gtcagcgcag ctgcatctgc gtgtaatgtc
    12541 acgccgcttt ccgccatttg tttgcttaat gcgggcagga agctatcggc gatgttttta
    12601 ttcaccagca acgtttcaac cgtattacat gtgctcggac gctgagtttt cgcgttgacg
    12661 atcactttta atgcttcagc gatctctgca ctttcatcaa cgtaaatatg gcatacgcct
    12721 ataccacctg tgatcaccgg gattgtcgac tgttcacggc acagtttatg caaaccagcg
    12781 ccaccacgcg ggatcagcat gtcgatgtat ttatccatac gcagcatttc actgaccagc
    12841 gcacggtcag gattatcaat cgcctgcacg gcacccgccg gtaagccaca ggatttcagg
    12901 gcgtcctgaa tcaccgccac cgttgccgcg ttagtgcgac aggtttcttt accaccacgc
    12961 aggatcaccg cattaccggt tttcaggcac agcgaagcga catcaaccgt cacgttcggg
    13021 cgcgcttcat aaatcacgcc aataaccccc agcggtacgc gacgacgctc aagacgcagg
    13081 ccgctgtcca gtacgccgcc atcgattacc tgccccaccg gatcggcgag gttgcacacc
    13141 tgacgtacat cgtcggcaat gcctttcagc cgtgcgggcg tcagtgccag acggtcaagc
    13201 atcgcttcgc taaggccatt ggctcgcgcg tcagcaacat cctgggcgtt agcgttgagg
    13261 atgatttcgc tttgtgcttc cagttcatcg gcgatttttt ccagcacgcg atttttttcg
    13321 cggctggaga gttgcgctaa tttatacgag gcttgcttcg cggcaatgcc catttgttcc
    13381 agcatcggcc agctccttaa cgggtaatca tgtcatcacg gtgaacggca accgggccgt
    13441 attcatatcc cagtattgca tcaatttctt gcgagtggtg tccggcaata cggcgtaatg
    13501 catcgctgtt gtaacgactg acaccgtggg cgatatcgcg accttcgagg ttgcaaatgc
    13561 ggatgacttc accacgcgag aaattgccag tcacgctttt aatgcctttc ggcaacaggg
    13621 agctgccgcg ttccagaatg gcggcagttg ccccttcatc taccgtgatt tcacccgccg
    13681 gcggcgcacc gaaaatccag cgtttacggt tttcaagcgg agtcgcctgg gcatggaaca
    13741 gcgtaccgac ggaaatgcct tccatcacat caccaataac gcccggcttg ctgcccgcgg
    13801 caataatggt gtcgataccc gcacggcaag ccacgtcagc ggcctgcaat ttagtactca
    13861 tgccgccagt tccgaggcct gaaacgctgt caccggcaat cgcgcgcagt gcgtcatcaa
    13921 tgccgtaaac atctttaatc agttctgcct gcggatttct gcgcggatca gcggtataca
    13981 aacctttttg atcggtcagc agcaacagtt tatcggcacc cgccagaatc gccgccagcg
    14041 cagaaaggtt atcgttatcg ccgaccttaa tctctgccgt agcgacagca tcgttctcat
    14101 tgattaccgg aacgatattg ttatcgagca acgcacgcag ggtgtcgcgg gcgttcagga
    14161 agcgttcacg gtcttccata tcagcacggg tcagcagcat ttgcccgacg tgaatgccat
    14221 aaatcgaaaa cagctgttcc cacagttgaa tcagtcgact ctgccctacc gccgccagca
    14281 gttgtttcga ggcgatagtc gctggcagtt ccgggtaacc caggtgctca cgtccggcgg
    14341 cgatcgcgcc cgacgtcaca ataacaatcc gatgcccggc ggcatgtaac tgcgcgcact
    14401 ggcgaacaag ttcaacgata tgggcacggt tcagacggcg cgatccgcct gttagcacac
    14461 tggtgccgag ttttaccacc agcgtctggc tgtcactcat gattctctgc cattcaattt
    14521 taggaaaaat gatatcaaac gaacgtttta gcaggactgt cgtcggttgc caaccatctg
    14581 cgagcaaagc atggcgtttt gttgcgcggg atcagcaagc gtagcggcag ttgtttacgc
    14641 ttttattaca gatttaataa attaccacat
//
LOCUS       DAJTRB010000096        14469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTRB010000096 DAJTRB010000000
VERSION     DAJTRB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.096"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(113..379)
                     /locus_tag="OO330_003273"
     CDS             complement(113..379)
                     /locus_tag="OO330_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5884037.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OO330_003274"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OO330_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5884038.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OO330_003275"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OO330_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OO330_003276"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OO330_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5884039.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OO330_003277"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OO330_003277"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5884040.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO330_003278"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OO330_003278"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5884041.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO330_003279"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OO330_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5884042.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OO330_003280"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OO330_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO330_003281"
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OO330_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5884043.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12641..14095)
                     /locus_tag="OO330_003282"
     CDS             complement(12641..14095)
                     /locus_tag="OO330_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5884044.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14469)
                     /locus_tag="OO330_003283"
     CDS             complement(14092..>14469)
                     /locus_tag="OO330_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5884045.1"
                     /translation="DGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGM
                     KPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAF
                     DDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3857 a         3650 c         3417 g         3545 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc agcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcggc
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgctg
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatc
//
LOCUS       DAJTRB010000097        14357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTRB010000097 DAJTRB010000000
VERSION     DAJTRB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.097"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..572
                     /locus_tag="OO330_003284"
     CDS             <1..572
                     /locus_tag="OO330_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5884046.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(634..1356)
                     /locus_tag="OO330_003285"
     CDS             complement(634..1356)
                     /locus_tag="OO330_003285"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5884047.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1358..2529)
                     /locus_tag="OO330_003286"
                     /pseudo
     CDS             complement(1358..2529)
                     /locus_tag="OO330_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2556..4172)
                     /gene="bglH"
                     /locus_tag="OO330_003287"
     CDS             complement(2556..4172)
                     /gene="bglH"
                     /locus_tag="OO330_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5884048.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4258..5652)
                     /gene="bglB"
                     /locus_tag="OO330_003288"
     CDS             complement(4258..5652)
                     /gene="bglB"
                     /locus_tag="OO330_003288"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5884049.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5671..7548)
                     /gene="bglF"
                     /locus_tag="OO330_003289"
     CDS             complement(5671..7548)
                     /gene="bglF"
                     /locus_tag="OO330_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5884050.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPKNDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7681..8502)
                     /gene="bglG"
                     /locus_tag="OO330_003290"
     CDS             complement(7681..8502)
                     /gene="bglG"
                     /locus_tag="OO330_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5884051.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKWLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQYAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8788..9513)
                     /gene="phoU"
                     /locus_tag="OO330_003291"
     CDS             complement(8788..9513)
                     /gene="phoU"
                     /locus_tag="OO330_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5884052.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9528..10301)
                     /gene="pstB"
                     /locus_tag="OO330_003292"
     CDS             complement(9528..10301)
                     /gene="pstB"
                     /locus_tag="OO330_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5884053.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10393..11283)
                     /gene="pstA"
                     /locus_tag="OO330_003293"
     CDS             complement(10393..11283)
                     /gene="pstA"
                     /locus_tag="OO330_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5884054.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11283..12242)
                     /gene="pstC"
                     /locus_tag="OO330_003294"
     CDS             complement(11283..12242)
                     /gene="pstC"
                     /locus_tag="OO330_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5884055.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12329..13369)
                     /gene="pstS"
                     /locus_tag="OO330_003295"
     CDS             complement(12329..13369)
                     /gene="pstS"
                     /locus_tag="OO330_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5884056.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13617..>14357)
                     /locus_tag="OO330_003296"
     CDS             complement(13617..>14357)
                     /locus_tag="OO330_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5884057.1"
                     /translation="NLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSIS
                     SGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQ
                     SCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSF
                     YATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFS
                     AAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3688 a         3680 c         3278 g         3711 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggtgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gatttttcgt agttatttaa ataatgagaa caggccggag
      601 cgtaattcat acatccggcc ttatttcttc agcttaaccg aacgctaatt ctgcggcagc
      661 ggctttatca gcgattacca tcagcgaagg atgcagctgc aacacggaag ctggaacatc
      721 ttcggtaacc ggaccttgca gcacattttt cagtgcctgc gctttacctg cgccactgac
      781 aattatcagc aagtttttcg ccgccatgat gcttttcggc cccatcgtca cgtagctgtc
      841 aggcactaac gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa ccatctctcc
      901 ctggatcggg aactccacgg tttgctcatg gaaatgggtc gtattcggca aattgccaca
      961 aaaatgacca tctgcaccta atcccagcac caccaaatcc aggccgcctt cacgcgccag
     1021 tttctgatca tgctcgcggt agttatcaat agtgagcttc tggatattct cttctttgat
     1081 ccccgcaggg gtgaaaaaca gattacgcag attggtaatc gttacgcctt ctccctcttt
     1141 gccgcgaaag ggaatttcat cgaaattata gaaatagcag ttatcgtacc aaggcttacc
     1201 tttaaccagg gtggtgaggt attcatacat gcctttgggc gtgctaccgg cggtaattgc
     1261 caggttaaca cgacgcgtct tcgacatata accaagcaga tggtgtgccg cgacacggct
     1321 catttcctgg taatcttcgg taatgattaa tttcatctta atgccttatt ttaaatttct
     1381 gtacgaaggc tgcataggca gcccgccaga catccatttc atggttcagg ccgggatatt
     1441 cctggtaatc aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct
     1501 tgccggttac gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa
     1561 tagctgccgg atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa
     1621 caccactgaa tgtggccagc caaagctttc cagatgattc attccggaaa ccagcgcctg
     1681 gtacccgcct tgtgaaagcc ctgccagcgc gcgaccatcg gcatctttac ggacactaaa
     1741 acgcttgcta atcagcggga taatatcgtt catcagttcg cgatctgccg ctttagcatt
     1801 cagcggataa aagactttac gtctttcctg aggcacgaaa tcttcgggaa taatgccctt
     1861 cgcatcggtt tcggtatccg ggatcaccac cagcatcggt ttaattttcc cttcagccag
     1921 caggttatcc atgatttgcg ggatacgccc ctgatcgata gcggaacgtc cggtatcacc
     1981 aaaaccgtga tagaaataga gcactggcaa aggctcgccc atgccggtgt atcctggcgg
     2041 ggtccagaca tacatctgac gttcagattg caatgcgttg gagtggtaag ttatggcgat
     2101 caaatcacca tgcgcaacag aacgcgtatc cagataactg cctggcacca gaatcatact
     2161 ggagttaacc tggcgctgag gcttagtcat tgcggtgcct gtatcaatgc tgcgtacacc
     2221 atcaacattg aaaaaatact cgtacaggtt gcctttcagg ataggtgtgc gccacgacca
     2281 gactcctgct tcgtctttgg tcatcgggtg aatattgtcc ggaaccggaa cacccaccac
     2341 tacagagaca ttttttgccc caggggcaaa gtagcgaaag gtcacgctgt tatcggcatt
     2401 gacctgagtc acatactgtt tcaccgggat agtgggtgcc gggctggctg gcatatccgc
     2461 tgctatggcc cactgagcag aaataccgct ggcaatagcc agcgttaaag cagcaatttt
     2521 tatattcatc gtaatattcc tttcattaag ttccattacc accagatttc agcctgggca
     2581 ccgacagcaa actgggcgtc tttattgtct tcgaaggtga agccatccag ttcgttttcc
     2641 agggctttga tatacgtggc gtagaaacga atttccagac gcgaggtcaa catactggta
     2701 ttgactttaa aggtatggaa gagcgtggtt ttataaccag actcattaaa tttattacta
     2761 ttcgcatctt tgttttgctg ggtgaaatag cccagttcaa cacctgtctg gttatattgg
     2821 tcccaaatat aggccgggcg aacaaccgca cgaatagatt cgaaatcaga gtgtgcgcct
     2881 gtttcgtagc tataaatatt gttaccgaag gagtaaacaa tcgcgttagc cacaataaaa
     2941 tgatcgccga tataggcttc gccctgcgag gtcagacgaa ctgctgttcc gccggtgtga
     3001 tcaccataat aacgaccatt aaaggtggta aatggacttg cgccagcata acggccaaag
     3061 ttcctggcga tagagttatt cgcaaccagg aaggagaatt cgttgaagcc acctttatca
     3121 aatttctgcg ttaaagatgt gccgaacatc caggtatctt tccagtcata atacccgtta
     3181 ttatcctgat tatctttttc cgatgcgctt tcgtttgccg tgacataacg accactcacc
     3241 attaaggtcg ctttatccca taacgggata tctttatagc gtaaatcaat agtatgggta
     3301 ttaatctgct gtttgttttg caggctgcga tcgtaatcat cgacatcttc gcgaaccagc
     3361 gcgatatcaa ttttacccgg accgactttc cagttttcca gacctacacc cgctgcagca
     3421 tcagtacgct gcgttttcca gtccagcatc tggatttcaa ttttcggcgc accgtgttta
     3481 cccacccaga aatcagcctc tggcgcaaag ggcaggaaac ctttggtggt aacgtacata
     3541 tcggagaact gcataaagtt ctcgccaccg gcattatcgc caaaccagcc ggtagagtac
     3601 tgctgaccaa cgttaccatc tatcatcaca acggcatcaa cccgtttgcc gttttcgttg
     3661 tagacacgtt gttttaactg caaatcaaac cagccggagt attcgttacc aaagcggccc
     3721 agagaaccaa tcgcccatga tttaggtgaa ccatgagagg cggtccccca accagaacgg
     3781 taatagccgt tgtaactaaa gccaatttca tctttcacaa acttactgaa atctttcaga
     3841 gtcatggaag aataaatcga gcctgtctca ctggcatttt tctcttcatt tttgaccagt
     3901 acagcatcag gttttgcggc actactggtc gggaacggat tggcggcata cccttgatca
     3961 ttcgtactta cgctacgatt caccgtcgct ggcgtatatt ttttcttctc ttcatcttta
     4021 tactttttga gttcgctttg cgtttctctt aacgcttttt ctaataactc aaggcgttgt
     4081 tccaccgtta atgattcagc caatgattgt gcagaaaagg ctaacggtgc cattaataag
     4141 atggcagagg taataagatt tcgtctaaac ataatatccc tttatggtgc aaagaaagaa
     4201 ttaacgtatc gcatccagac tgttctgaat gcgacgataa ttaaggtgct ttattggtta
     4261 tttttttaat gacagccccc gcgttttgat cacttctgca taccatccga agcttttctt
     4321 acgcgttctt gtgaggcttc cttcgccatt atcatcacga tccacataaa taaagccgta
     4381 gcgcttagac atttgtgaat gagatgcact gactaaatca attggccccc aactggtgta
     4441 ccccataata tccacaccat cggcaatcgc ttcatttacc tgtaccaggt gatcgtttaa
     4501 ataggcaatt cgataatcgt cctgtatcga accatccgct tcaacgctgt cttttacgcc
     4561 taatccattc tcgacaataa ataacggttt ttgataacga tcccaaagcg tatttaacag
     4621 aacccgtaat ccaaccggat caatttgcca cccccactct gaacttttca gatgcggatt
     4681 ggggatcata ttcagtatgt tgccctgcgc atttttatta atgctttcgt cgtgggaaac
     4741 acaaccagtc atgtaataac taaaagagat gaaatctaca gtatgtttta aatcttctgc
     4801 gtcactttca gtcatctcaa tggtgatatt gtggtcgcgg aagaaacgct gcatatagcc
     4861 gggatattgg ccacgcgcct gaacatcacc aaagaacatc cagcgccggt tctcttccat
     4921 ggcctgcaac atatcctgtg gctggcaggt gagggggtaa accagcccac cgagaagcat
     4981 attgccgatt ttcgcttcgg ggatcaggct atgataggct ttaactgccc gcgcactggc
     5041 aaccagttga tgatggatcg cctgataaac ttccgcctcg ccactctctt ctgccagccc
     5101 cacgcccgtg aatggcgcgt gtaacgacat attgatttca ttaaacgtca gccataacgc
     5161 tactttatgt tggtagcggg taaagaccgt gcgggcgtaa tgctcgaagt gatcgatgac
     5221 cgcccgatta gcccaaccgc cgtagttttt caccagccca tatagcattt cgtaatggga
     5281 taacgttacc agcggcttga tccccgcctg cgccatttca tcaaacagcc gatcgtaaaa
     5341 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
     5401 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
     5461 acggtaataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
     5521 catttttccc attacgccat gaggctgtaa atctgaggtc gtgatccctt tgccatcttc
     5581 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
     5641 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
     5701 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
     5761 taataccggc gtcgtcagat caaatccggc ctcgcgaata gcagggatat caaaagaaat
     5821 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
     5881 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
     5941 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
     6001 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
     6061 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
     6121 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
     6181 acgtttactg gtgatgaaat gaagcatcaa cgtaccaaca aatgcgcaac ctatggcaat
     6241 gacaccgcca ataacgctgg cccagacggt gaaatcaatc cccgttgacg ggatggtctg
     6301 catgaaggtg aaaatacttg gcaaaccaaa ggagtaaact ttcgtttgcg cgtagccaat
     6361 gatggtggcc cccaatgctc cactgataca ggcgataaca aaggggtact tacgcggcag
     6421 gttgacgcca tataccgctg gttcggtgat accaaacaga cccgtcaacg ccgctgatcc
     6481 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     6541 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     6601 gttattgata cacagcggca ccaggcccct gtgcagtccg aacatgacga agatttgcca
     6661 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     6721 ggcggcaatc atttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     6781 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     6841 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     6901 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccggcgcatc
     6961 cgctttttgc ccgttctcga aggcagtcag aattaatgga tgcactaacg ctccaccaat
     7021 caccatggca gtaaatggat taccgccgaa gcgtttgccc gcggtgtatc ccaggattat
     7081 cgggaagaac ccaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     7141 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     7201 cgccatcaga ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     7261 cagattacct ttctcatcat ttttcggtgc ctgttgcgct ttttcgccaa ggcctgccac
     7321 gctgttaacc gccaggaaga catcggccac atggttacct atgaccacct gaaactgacc
     7381 accgctttcc accaccataa taataccggg ggtctttttc agtacctcag cttgcgcttt
     7441 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac tcacaatgtt
     7501 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     7561 accgctttgc ggcaaaactc caaaaaaaac ctgaaaaaaa cggcctgacg tgaatcaagc
     7621 aatttttttc aggttttgcc cgcttagtgc ggtaacaatc ctttactcag taataatatt
     7681 tcagtgttct ttgcgcacgc gctctatatt tatggctaaa aacataatct ctgcgggtga
     7741 aattttacgt tgatactgca aaccaataaa aatggcgatc cgttccgcat attgccatgc
     7801 ttgcgggtaa ttttgtttta ctgcttgttg taatgattca tcactatcgt taattgaagc
     7861 atgttcaaga atacgccagg ataaaaactt cagatgtgta accagtcgct gataactcaa
     7921 gctttcttcc tggtaattaa ggctgaactg aaattttatt aattgcagca tttcgcgcat
     7981 taactgcgtg acacctgcaa catcctccat atttccgctc atttgggcac tgaccagatg
     8041 catggcaata aagcccactt catctttcgg taactgcacg cccaaccatt tatcaataat
     8101 ggttaacgct tcttccccta gctggaactc tttcgggtaa agccgctgga tatcccacag
     8161 caacacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta gcgagatata
     8221 aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa gagataagac gatcacaggt
     8281 tgccatcacc tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt tcagttcatg
     8341 actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc cagcgcgttt
     8401 ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat caataaccac
     8461 cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga ccttttttat
     8521 aaacaaaaaa aaccgacttc accagtattc tctggttatg tcaggttttg cctgcgaatg
     8581 cagtaacaat ccagtcattt attaatggtt tttataacga acatccaggt tcggaaatta
     8641 atttaattgc gtgcagagaa aatagcaatg cgctattgat aaaaatatga ccatgctcgc
     8701 agttattaac tttgtgtaat tttaggaatt tataaagtta tatataacaa atcccaataa
     8761 ttaagttatt gggatttgtc tggtgaatta tttgtcgcta tcttttcccg ccagcagttt
     8821 atccagctca tcaccgccta catgacggaa atcctgaccc ttcacgtagt agaagataaa
     8881 ctcacaaata ttctggcagc ggtcgccgat acgttcgatt gaacgcgcac agaacagcgc
     8941 ggtaagtacg ctcggaatag tgcgcgaatc ttccatcatg taggtcatca gttgacgaac
     9001 aataccttcg tattcctgat cgactttttt atcttcacga taaatacgta ccgcttcgtc
     9061 aatgtccatc cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg tatgacggcc
     9121 cagcgactcc agacttacca gcaacggctg atgctgctgg gagaatttct ccagcgcagt
     9181 acggcagatt ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg ttttactgat
     9241 caccataacc agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa tgcgcacgca
     9301 ggcttcatcg atcgccactt ccatcatgtt gacgttcttg tcgccttcga tcacgcgctt
     9361 cgccagatcg ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct gctgctccac
     9421 catgccgccc atggtcatca cctgcgtgcg gatactttcc agttcggcgt tgaactggcc
     9481 ggaaatatgt ttattaagat tgagactgtc cataacgcac tcctgaatca accgtaacga
     9541 ccggtgatgt agtcttctgt ttgtttcttc gctggcttgg tgaacagatc gtccgtgttg
     9601 ctgaactcaa tcaattcgcc caggtacata aatgccgtgt ggtcggaaca acgcgcagcc
     9661 tgctgcatgt tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc
     9721 tcttcaatac gcccggtaga gatggggtcg agcgccgaac acggttcgtc gagcagcagc
     9781 acttccgggc gaatggcgat accacgcgca atacacagac gctgttgctg accaccagag
     9841 agagagtaac cgctctggtg caatttatct ttggtttcgt tccacaatgc ggctttggtc
     9901 aatgcccact gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa cagacgaacg
     9961 ccaaaggcga tgttgtcgta gatggacatc gggaacggcg tcggtttctg gaacaccatg
    10021 cccactttcg cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt gtcgccatca
    10081 agcagaattt caccttccgc acgctgctcc gggtacagtt caaacatttt gttgaaggta
    10141 cgcagcagcg tcgatttacc gcagccggac ggcccgataa acgccgtaac ctggtttttg
    10201 gcgatatcca agttgatgtt tttcagggca tggaatttgc cgtagtagaa gttcaaatta
    10261 cgaacctgaa ttttactcgg ggcagtttca accatactca ttgcaatctt ttcctcatcg
    10321 tacctgatgc ttttcgctaa tcaggcttac cgtagggccg ggtaaggcgt tcacgccgca
    10381 tccggcaaag tgttaaccgt gtttattctt cgcaaaaaca acgcgcgcca gaatgttcag
    10441 cagcaggacg cacagggtaa tgatcaatac cccggcccag gccagttgtt gccattccgc
    10501 aaacgggctc atcgcaaact taaagatcgt caccggcagg ttggcgatcg gctgcatcat
    10561 gtccgtgctc cagaactggt tggagagcgc ggtaaacagc agcggcgcgg tttcaccggc
    10621 aatacgggca atcgccagca ggataccggt cataatcccg gacaccgacg ctttcagcgt
    10681 aatcgcggag atcatcttcc acttcggtgt acccagcgca taagccgctt cacgcaggct
    10741 gtccggcacc agtttcagca tgttctcagt ggtgcggata acgatcggca cctgcaacag
    10801 cgccagcgca attacgcccg cccagccgga gaagtgctcc atctgtgcca ccacgatggt
    10861 gtagacaaac agaccaacca caatcgacgg cgcggagagc agaatgtcgt taatgaagcg
    10921 aatcacttct gccagccagg atttacgacc atattccgcc agataaatcc ccgccataat
    10981 gcccagcggc gtaccgaata ccgtggccca caaaattaac agcccgctac ccgccagggc
    11041 gttcgccaga ccaccacctt ccgtattggg cggcggcgtc atttcagtga acagcgccag
    11101 cgacataccg tcgataccgc gagtgatggt ggacattaaa atccagatca gccagaacag
    11161 gccgaaggcc atcgtcgcca tcgagagcgt cagcgcaata cggtttttga ggcggcgacg
    11221 cgcctgcatt ttgcggcgag attcagccag cgccgcagtg gtttgcattt caaccatagc
    11281 cattagcgtg ccccctcatt cttagccagg cgcataatca taaacttcga tgcggcgagg
    11341 acgatgaagg taatcacaaa caggatcagg cccagttcca tcagtgcggc aacgtgcaga
    11401 ccggattccg cttccgcaaa ttcgttcgcc agcgcagagg tgatactatt gcccggcatg
    11461 tacagcgagg cgctgtcgag ctggtaggtg ttaccgatga taaaggtcac cgccatggtt
    11521 tcaccgagcg cgcggcccag ccccagcatg atgccgccga taacaccatt tttggtgaac
    11581 ggaagaacga tacgccagat aacttcccag gtggtgcagc caataccgta ggccgactct
    11641 ttcatcatca ccggggtttg ttcgaacaca tcacgcatta ccgccgcaat gtacggaata
    11701 atcatgatgg cgaggatcac gcctgccgcg aggataccga taccaaatgc ggggccagag
    11761 aacagcgcgc caacaatcgg gatattcgac atgatattgc cgaccggctc ctgaaagtaa
    11821 acggcgaaca gcggcgcaaa gataaacagg ccccacatgc cgtaaacgat acttggaatg
    11881 gctgccagca gctcaatggc gatacccagc gggcgtttca gccagccagg cgcaagctca
    11941 gtcaggaaca gggcgatacc gaaactcacc gggacggcga tcagcagcgc gataaacgaa
    12001 gtcaccaacg taccgtagat cggcaccagc gccccgtaga tatcgttcgg tgcatcccac
    12061 tctttggtcc ataggaaagc cagaccaaat ttctgaatgc tcggccagga ggagatgatc
    12121 agagagacaa taatgccacc caacatcaat agcacaatca gcgccgccag ttttaccagc
    12181 acgctgaaaa ttatgtcgcc ctttttaccc ggtgggttaa aagcgggctt ggttgcagcc
    12241 ataagttact cttcagttaa acgcgtttac aaagtcattt tgtaatgccg gatgcggcgt
    12301 aaaacaccgt acccggcctg gagttttatt agtacagcgg cttaccgcta ctgtctttaa
    12361 tattggtctt ccacgcagcg cgaacctgtt caactacact atccggcagg ctggcgtaat
    12421 ccaggtcgtt cgcctgttta gccccggttt tgtacgccca gtcgaagaat ttcagcacct
    12481 ctgtgccttg ttctggtttc ttctgatctt tgtggatcag aatgaacgtg gtagaggtaa
    12541 taggccatgc atcttcgcct ttctggttgg tcagatcctg agcgaaggtt ttgctccagt
    12601 ctgcaccttt tgctgcatta gcgaagtttt cttcggtcgg actaaccggt ttaccatcag
    12661 cggagatcag tttggtgtac gccaggttgt tctgcttcgc gtaagcatat tcaacataac
    12721 caattgcacc cggcagacgc tgaacgaacg cggcgatacc gtcgttacct ttaccgccca
    12781 gaccgatcgg ccattttacg gtagagccag taccaacgtt gtttttccac tcttcgttca
    12841 ctttcgccag gtagctggtg aagacgaagg aagtcccgga gccatctgcg cggcgtacta
    12901 cagcaatgtt ttgtgaaggc agtttcagac ccggattcag tttggcgatg gcttcatcat
    12961 cccacttctt gattttgccc aggtagatgt cgccgagggt tttaccatcc agcaccagtt
    13021 cgccagactt cagccctgga atgttaaccg ccagcaccac gccgccaatc acggtcggga
    13081 actggaacag accttcctga gccagttttt cgtcagacag cggcgcgtca gaggcaccaa
    13141 aatcaacggt attagcgata atctgtttta cgccaccgga agaaccgata ccctggtagt
    13201 taactttatt accggtttct ttctggtaag tgtcagccca tttggcatac accggcgcag
    13261 ggaaggttgc acctgcacct gtcaggcttg cttctgcaaa cacagagaaa gcactcatcg
    13321 ataaggtcgc ggcgacaaca gttgcgacgg tggtacgcat aactttcata atgtctcctg
    13381 ggaggattca taaagcattg tttgttggct acgagaagca aaataggaca aacaggtgac
    13441 agttatatat aaggaatatg acatttttat gacagaaagg aattaccaaa taacttactg
    13501 attttaaatg tgaagatgaa aatcccacat gccatctttt tgtcatgatg catttaaaag
    13561 tgataaagaa atgggtgaga tgacaacgat gtgatgaaca atcgttgtca gacctattag
    13621 cgcacaatct caacagttat cgtcgctgtt gcagtaaatt gccccggggc tgggcgagct
    13681 ccagtaaggc tcgcaggagc agctgaaaaa gttacactac ctgatttttc accatacact
    13741 gttgatttat ccatgtctgt tggcaactca ccaccgttca ctttgatttc cttattattt
    13801 ttgtcgtaaa tttttatccc cacgttagag tttgaagtta cgaccattga attgtccgta
    13861 ttgggttcgg tagcataaaa agacgtactg atatcatcgg tattcagtat ccccaggttt
    13921 tcacactcaa tagtgactgt tttagtaatt ttatgtgttg ttaccgcact tccaacaaca
    13981 gatgaaaaat ctgcagctgg tatttcttta aaatcaaaac aaactggctt taaattattt
    14041 atttcgcaac tttgcggagc tgtaatatcg ccccgaatat aaacatcagc catcggctgt
    14101 gagtaatcca ccggggtgtc acggctaatt gttccataca atgtcactat ctttgtttca
    14161 ggaataatca attttcctgc aaatgtcttc ttgatataaa aatatactat cgctttagcc
    14221 ccactggata tacttaaagg ttcaatgcgg ttgcaattgt actttgtatt accagacggg
    14281 ttgttagcat gatattcaaa aggtgcattc acatacccaa ccccaagaat atctatagca
    14341 atcccaacat caaggtt
//
LOCUS       DAJTRB010000098        14313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTRB010000098 DAJTRB010000000
VERSION     DAJTRB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.098"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            21..3075
                     /locus_tag="OO330_003297"
                     /pseudo
     CDS             21..3075
                     /locus_tag="OO330_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3085..6731
                     /locus_tag="OO330_003298"
                     /pseudo
     CDS             3085..6731
                     /locus_tag="OO330_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6792..7199
                     /locus_tag="OO330_003299"
     CDS             6792..7199
                     /locus_tag="OO330_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884058.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7741..8829
                     /locus_tag="OO330_003300"
     CDS             7741..8829
                     /locus_tag="OO330_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5884059.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVITVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8840..11091
                     /locus_tag="OO330_003301"
                     /pseudo
     CDS             8840..11091
                     /locus_tag="OO330_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11084..12220
                     /locus_tag="OO330_003302"
     CDS             11084..12220
                     /locus_tag="OO330_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5884060.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            12217..14220
                     /locus_tag="OO330_003303"
     CDS             12217..14220
                     /locus_tag="OO330_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884061.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3550 a         3746 c         3652 g         3365 t
ORIGIN      
        1 agtaagttgg agtcattacc aaatgggatt atgcgaataa tgtcattatt attctgccgt
       61 ctggcattgc acctgactac ttggagcaat attccagatt tgaattacgc ctacgtaaac
      121 atttatcact ggcggaagag tctctctggc aaaaatgtgc acaaaaactt attgccgcaa
      181 ttccacatat tccagaatgg cggcaaccat taattgcttt gttattaccc gaaaaaccag
      241 aaattgcaca tgaaattgcc cagcgtctac tggggcaaaa aaaattaccc tcgcttgagt
      301 ggttaaaaat agtggcgact gatgagcaca ttcttgcctc attagaaaaa tatcacgagc
      361 catatgccat ttttgatgat tactattgtg gtgcgatatg gtcagccacc gtattacagg
      421 agcaaggtgt tgcagccctg ccccgatttg ctccctatgc cgcaagtgac tactgcgccg
      481 atgtgttgcg tcatatcaat catccgttcg cattaacact gcttatacgt gtagccgggc
      541 atactaaacg ctgtcacgat cgaatgacga aagcctgcgc tgcgttccca cacgcagcac
      601 tggcagcact ggcggaactt ctagtgcaaa aagaagagaa tagttggcgc attatgctaa
      661 tgacgatgtt tatctcgcaa ccagcactgg cagaacaggt cattccctgg ctttcgacac
      721 ccgcagttgc agtgctgaaa tcatgccagc accaactgac acagccctca aaccatgcca
      781 gcgccgatct actgccagcc atagtagtct ccccaccatg gatttcgaaa aagaaaaaat
      841 cgccgattcc ggtgctggat ttagcgccat taggcatcga gccaatctgt tatctgacag
      901 aagaaatcag taatcaactt ttggcgaaat atatctggta ttcaaaacac atcacggtta
      961 gccatgaaga aagtactacc aacctgttgg caaggatggg ttttcaacga cggatcgctg
     1021 gtacatatat taaagctccc gaagcggtag ttgaggcatg gctaaatgaa gattattcaa
     1081 ccttactaag tgaatttaag gtgtttcatt cacctaccgg gcattattgg cagttgggga
     1141 ttttgacaac attgccgctg gaggctggag aaagcagtaa aagcatggaa tgcccttact
     1201 ctatctccac ataccgatac cgaatacgcc atgttacatt ttggactcaa aggtttactt
     1261 ggtttagtta actcaattac acgctatcca caggaagcct tgcccatcac gaattacttc
     1321 gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca acaaactcaa aacgctacgc
     1381 gaaaacgccc gcacctggct gttgaaatac ccggaacatg cccttaccgg cctgctgcct
     1441 gcggcgctcg gcaaagccgg tgaagcacag gataacgccc gcgctgcctt gcgtatgctt
     1501 accgaaaacg gtcatcagcc attactgcaa gaaatcgccc ggcgttataa ccagtcggaa
     1561 gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct tagataatca cccgacaaaa
     1621 atccccactc ttccgacctt ttatcagcca tcgatctgga cgcgcccggt attaaaagca
     1681 aacgcccaat cactgccaga taacgccctc ctccacctcg gcgaaatgct ccgcttccct
     1741 caggaagagg ctctgtatcc gggattattg caggtgaaag acgcctgtac cgctgactct
     1801 ctggctgaat ttgcctggga tctgtttacc gcctggcaga ccgctggcgc gccgtcgaaa
     1861 gagagttggg cgttcactgc gttaggcgtt ctcggtaacg atgacaccgc ccgcaaactg
     1921 acgccattaa tacgcgcctg gcctggtgaa tcccagcata aacgcgccac cgttgggttg
     1981 gatattctcg ccgctatcgg tagtgatatc gccctgatgc agcttaacgg cattgctcag
     2041 aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa agattgccga cattgccgag
     2101 agccgcgaac tcacggtagc ggagcttgaa gatcggttag caccggatct cggtctggat
     2161 gataacggtt cgttgctgct ggattttggc ccacgccagt tcaccgtcag ctttgatgaa
     2221 accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc tgaaagacct gccaaaacca
     2281 aacaaaagcg atgatgagtc acaggcgaac gatgctgtta accgctacaa attgctgaaa
     2341 aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc ttgaatccgc catgtgcctg
     2401 cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg ttgtgcatcc gctggttcgc
     2461 cacttaaccc gccgtctgat ttggggcgtt tatagcgccg acaaccagct actggcctgt
     2521 ttccgggtag cggaagataa cagctacagc accgctgacg atgatctttt caccctgccg
     2581 gaaggcgata tctctatcgg cattcctcac gttctggaaa tatcaccgac ggatgctgtc
     2641 gcctttggtc agctttttgc cgactacgaa ctgctaccac cgttccgtca gcttgaccgt
     2701 aacagttacg ccctgacaga agccgagcgc aacgccagcg aactgacccg ctgggcaggc
     2761 agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata aaggctggat aaagggcgaa
     2821 ccacaggatg gaggctggat gatcaaacct ctcggtcgct ggtcgttaat catggaaatc
     2881 gacgaaggtt ttgcggtggg tatatcacca gccgaactta gcgctgagca gctcttaagc
     2941 aagctgtggc tatgggaagg caaagcggaa agctatggct gggggagtaa ttcaacacag
     3001 gaagcgcagt tctccgtact cgatgccatc accgccagcg agctaattaa cgatattgaa
     3061 gccctgtttg aataaggaaa gagcatggac aaggaattac cgtggctggc ggataacgcc
     3121 caactggaac tgaaatataa aaaaggcaaa acaccactca gtcatcgcaa ttggccgggc
     3181 gaaccagtgc ctgttatcac tgaaagcatc attcagacac tgggtgatga attgctacaa
     3241 aaagctgaga agaaaaaaaa cattgtctgg cgttatgaaa atttttcacc ggagtggcag
     3301 tccgccatta cgcaggccat caacttgatc ggcgaacaca aaccctcagt cccggcccgc
     3361 acaatggcgg cactagcctt tatcgcgcaa aatgacaacc aacagttgct cgacgaaatc
     3421 gtccaacaag aggggctgga atatgcgact gaggtggtga ttgcacgcca gtttattgca
     3481 cggtgttatg agagtgatcc tctggtagtc acattgcagt atcagaacga ggactatggc
     3541 tatggttatc gctcagaaac ctataacgaa ttcgatctcc gactgcgtaa acacctctct
     3601 ctggcagagg aaagctgctg gcagcgttgc gccgacaaac tcattgccgc actgccagga
     3661 ataaccaaag ttcgccgccc ttttattgcg ctgatcctcc ccgaaaaacc agaaatcgcc
     3721 aatgagttgg taagccttga atgcccgcgg actcattttc attctaagga gtggttaaaa
     3781 gttgttgcta ctgaccccaa agcggtgaga aaactcgaac gctactggag ccaggatata
     3841 tttagcgatc gagaagccag ctacatgtcg catgaaaacc acttcggcta cgcggcctgc
     3901 gctgcccttt tgcgcgaaca aggactggca gtcgttccgc gcctcgcgat atatgcccat
     3961 aaagaagatt gcggcagtct gctggtacaa attaaccatc cgcaagtcat ccgcaccttg
     4021 ctgttagttg ctgataaaaa caaacccagc ctgcaacgtg tggctaaata cagtaaaaac
     4081 ttcccccatg cgacactcgc cgcactggca gaactgctgg cgttaaaaga accaccagcc
     4141 cgcccaggtt atccaatcat cgaagacaaa aagctgcctg cacagcaaaa agctcgagat
     4201 gaatactggc gtacgctgtt acaaacgctg atggcatcgc agccacaact ggcagcagaa
     4261 gtgatgccgt ggttaagtac tcaagcccgg gcagtgctga atagttattt atcggcaccg
     4321 cccaaaccag ttattgatag caccgataac agccaaatgc ctgaaatcct cgtttcacca
     4381 ccatggcgtg ggagaaagaa aaagacttcc atacgtcact tttcgctgcc tgatcttacg
     4441 cttgcaccgc aaacgtacag gtcgccgaat aaacactgga cgccggaaca gcaaactgca
     4501 attgaccatt ttagcgccat gtcttttgac gaacgtcttg ctacgagagg agcggatatc
     4561 ttcttgcggg aattaggttt tgacgttcat agcctggaat ttaataagcg tcttcttaat
     4621 ggacagttgg atgcctttga tactcagcaa ctgcaagaat acacatcaat aggactattg
     4681 cgtacttatc acgcgcatca ggccaaatct aaacagtacc gggaagacgc caccaatgca
     4741 ttactggcac aagatagcat tgcgttgatg ggagggtggc gaacttttca gcacggattt
     4801 tactatggta atgaacagcg ttgggggatt tggaatctgt accttatcgc gcagatgccg
     4861 cgcgaaatgg cggtatcgtg ctggcaacaa attgtcgagg ctgattttca ttacaccggg
     4921 gtggaatatt tgttaagtgt gctgggtacc gatgctttgc ctggattaaa caccgccttt
     4981 gcccgacatc caaaagagat atttccgctg ctgattcatt tcggcgcaac agaactggcc
     5041 ctgcccattg cccgcgtctg gcaccttttt gcgggccagc gtaatctggc tcgccagtgg
     5101 attttacaat ggccggaaca tacggctact gcacttatcc ctcttgtctt caccaaatct
     5161 agcgataaaa gcgaagctgc attacttgcc ctgcgtttac tttacgaaca tgggcatggc
     5221 gaatggctac aaactgtagc caatcgttgg cagcgtacag atctatggcc tgccctggaa
     5281 caattactta aacagagtcc aatagagatt tacccggcac gcattccaaa agcccctgat
     5341 ttctggcatc cgcaaatgtg gtctaggccg cgccttatca ctaataatca acctgttacc
     5401 gatgacgctc tggaaattat cggcgaaatg ctgcgcttta cccagggggg acgtttttat
     5461 agcgggctgg aacaactgaa aacgttctgc cagccacaaa cgctggcagc ttttgcctgg
     5521 gatctcttca ctgcgtggca acaagctggt gcccccgcaa aagacaactg ggcatttctg
     5581 gcgttaagtc tctttggtga cgaaagcacg gcacgggatc taacgacaca tatcctcgcc
     5641 tggccacaag aaggcaaatc tgcccgtgcg gtcagtggca tgaacatcct taccctgatg
     5701 aataatgata tggcgctgat gcagctgcat catatatcgc aacgggcgaa atcccgcccc
     5761 ttacgtgata acgcggcgga atttcttcag gtagtcgcag aaaatcgcgg gctaagccag
     5821 gaagagttag cggacagatt agtcccaaca ctgggcctta atgatccgca ggcgttgagt
     5881 tttgattttg gtccccggca gtttaccgtt cgcttcgatg aaaacctcaa cccggttatc
     5941 tttgatcagc aaaacgttcg ccagaaaagt gttccccggt tgcgcgccga tgacgatcaa
     6001 ctgaaagcgc ccgaggcact ggcccgacta aaagggttaa aaaaagatgc tactcaggtg
     6061 agcaaaaacc tgctcccgcg tcttgaagct gccctacgta ccacccgacg ctggtcgctg
     6121 gcagattttc attctctgtt tgttaatcat ccctttaccc gtctggttac ccagcgatta
     6181 atatgggggg ttatccggca aatgaaccgc gtcgtttact caacgccttt cgtgtggccg
     6241 cagaggggga gttctgcaat gcgcaagatg agccaattga cctgcctgcg gacgctctga
     6301 ttggcattgc ccacccgtta gaaatgacag cagaaatgcg cagtgaattt gcacagcttt
     6361 ttgccgatta cgaaattatg ccgccttttc gccagttgtc gcgccgcacg gtgctgctca
     6421 cacctgacga gtcaaccagt aacagcctga ctcgctggga aggtaaatcc gctaccgttg
     6481 ggcaacttat gggaatgcga tacaaaggct gggagtcagg ttatgaggac gcatttgtct
     6541 atgacctggg tgagtaccgg ctggtcctta agttttcacc cggttttaac cactacaatg
     6601 ttgatagcaa agcgctaatg agcttccgtt ctcttcgagt gtaccgtgac aataaatccg
     6661 tcacttttgc cgaacttgat gtgtttgatt tgagtgaggc gttaagcgca cctgacgtca
     6721 ttttccatta aaacaaagcg ggcagtgatg cgactgcccg ttatctacac gacttaccag
     6781 cggggaaagc gatgatcgtg caaaaagagc tggttgctat ttacgattat gaggtccctg
     6841 tacctgaaga tccgttttcc ttcagacttg agatccataa atgctctgaa ttatttacag
     6901 gttccgtcta ccgactggaa cggttccggc tacgtccaac atttcatcaa cgtgatcgag
     6961 aggatgttga cccgctaata aatgatgcgt tgatttatat aagagatgag tttattgatg
     7021 agcaaaaatt acgaggcgaa tcacctgaac ctgtaatagt aatttttaat tgtaaactac
     7081 agaatatatt caaccaacaa atagaattat atactctaga taattcaagt cgcagcacat
     7141 gcaacttgaa gtatgacgag tatagcttta ttaaaggggg gaacggtatt cccccttaga
     7201 tgcgtttcaa tttgcccgtt acttcaacaa atcaatcaac cgtaacagag ccactaatag
     7261 ctccagtaca gcaatgagca cggttaactt ttgtgtcagc gtgtccatcc gctctcctta
     7321 ttaaggagca caacaccagc accttaccct tacactgcct gtcgatactg gtttacgttt
     7381 gttgagtcgc catgttgcac tccggatcag agcactacct gcggcaccac ccgtccggta
     7441 gccaggactt gaaataggtt cgatccggcg caacttccca acagtcagga ttataaatct
     7501 cattatctta attgcaacac tgtccagagg tggttataat tcgttcgttg gaacacgatg
     7561 ttgcacaggc agtggtgtta ggcctgaaaa tagtaaataa aagaacctgc ctcaccagca
     7621 gtttttttta tttactgtga tctgctttcc agatattttt cgctcaaaca actaatgcgc
     7681 caaacattta ttgcgcgtaa aatatcgttt atttcattaa tacatttcag ggatgaatat
     7741 atgtcaccgc agaataatca tctgcagcgt ccgcctgctg ctgtgttata cgccgatgaa
     7801 ctggcaaaat taaaacaaaa tgataacgca ccttgcccgc ccggttggca gttaagtttg
     7861 cctgcggccc gtgcttttat ccttggcgat agcgcgcaaa atattagccg taaagtggtg
     7921 attagtccct ccgctgtcga acgtatgtta gtcactcttg ccaccggacg cggtttgatg
     7981 ttggtggggg aacctggcac cgcaaaatct ctcctgtctg aattactggc aacctctatt
     8041 agcggcgatg ccgggttaac catccagggc ggcgcatcta ctaccgaaga tcaaatcaag
     8101 tacggctgga actacgccct gcttatcaat catggtccat caacggaagc gctggtccct
     8161 gccccgcttt accagggaat gcgcgacggc aaaatcgtcc gttttgaaga gataacgcgt
     8221 acaccgctgg aagtgcaaga ctgtctgtta ggtatgcttt ctgacagggt gataacggtg
     8281 ccggaactca ctggtgaagc cagtcagctc tatgcgcgag aaggctttaa tattattgcc
     8341 actgccaata cccgcgatcg cggcgtcaat gaaatgagtg ctgcgctaaa gcgtcgcttc
     8401 gattttgaaa ctgtctttcc gattatggat ttcgcccagg agctggagct tgtcgccagt
     8461 gcttcggcgc gtttgctggc ccatagcggt attccgcata aagtgcctga cgcggtactg
     8521 gagttactgg tccgcacctt ccgcgatctg cgtgccaacg gcgaaaagaa aacgtcaatg
     8581 gatacactaa cagcgattat gtccaccgct gaagccgtga acgtggccca cgccgtaggc
     8641 gtccgcgcct ggtttttagc gaatcgcgca ggtgagccag ccgacctggt ggactgcatt
     8701 gccggaacca tcgtcaaaga taacgaagaa gatcgcgctc gtctgcgccg ttacttcgaa
     8761 cagcgtgtcg ccacacataa agaagctcac tggcaggctt attatcaagc ccgccaccgc
     8821 ctgccgtgag gaaagatgca tgagcgagcc gttaattgtc ggcatccggc atcatagtcc
     8881 ggcctgcgcc cggctggtga aatcgttaat cgaaagccag cggccacgat acgtgttgat
     8941 tgaaggccct gctgatttta atgaccgggt agacgaactg tttttagccc accagcttcc
     9001 ggtagctatt tacagttatt gccagtatca ggacggtgca gcccccgggc gtggtgcctg
     9061 gacgccattt gctgaatttt cgccggagtg gcaggcgcta caagccgatc gtcgtattca
     9121 ggcacaaact tacttcatcg atttgccttg ctgggcgcag agtgaagaag aggacgattc
     9181 gcctgatacg caagaggaaa gccagacctt gctgctgcgt gctgggatca cttgttcgaa
     9241 gatgaaagcc agcaaactgc attaccctct gcactggcgc actattttgc ccaactgcgg
     9301 ggcgactccc ccggcgatgc tctaaatcgt cagcgcgaag cctttatggc ccgctggatt
     9361 ggatgggcga tgcagcaaaa taatggcgac gtgttagttg tctgcggtgg ctggcacgct
     9421 ccggcactgg caaaaatgtg gcgcgaatgt ccgcaggtaa ttaacacgcc agaattgccg
     9481 tcgctggcag atgccgttac aggttgttat ctcaccccct acagtgaaaa gcgccttgat
     9541 gtgctggcag gatacctttc aggaatgcct gccccggtct ggcaaaactg gtgctggcag
     9601 tggggcttac agcaggcggg agaacaactg ctaaaaacgg ttctcacccg tttgcgccag
     9661 cacaagttgc ctgcttcgac cgcggatatg gctgccgctc atctgcatgc gatggcactg
     9721 gcacagttgc gcggtcatac actaccgtta cgcactgact ggctggatgc catagcagga
     9781 tcgctgatta aagaagccct gaatgcgccg ttgccgtgga gctatcgcgg cgttattcat
     9841 cccgataccg atccgattct gctaacgttg atagacacat tagcgggtga cggattcggt
     9901 aaacttgccc cttctacgcc acaaccgcct ctgccaaaag atgtcacttg cgaactggaa
     9961 cgtaccgcaa tctctcttcc ggcggagctt accttaaatc gctttaaccc caatggacta
    10021 gcgcaaagtc aggtgttaca tcggctggca atactggaga tcccagggat tgtacgccag
    10081 cagggaagca cactgacact tgcaggcaac ggtgaagaac gctggaaatt aacccgcccg
    10141 cttagccagc atgcggcatt gattgaggcc gcctgctttg gtgccacact ccaggaagcc
    10201 gcacgccata aattagaagc cgatatgctg gacgcgggtg gaatcggcag cattaccaca
    10261 tgtcttagcc aagcggcgtc agcgggtctg gcgtccttca gtcaacaatt actggagcaa
    10321 ctcacattat taatcgccca ggaaaatcaa tttgccgaaa tgggccaggc gctggaagtg
    10381 ctatatgcct tatggcggct ggatgaaatt agcggtatgc aaggcgcgca gatattacaa
    10441 acgacgttat gcgcggctat cgatcgcacg ctgtggctgt gtgaatccaa cggcagaccg
    10501 gatgaaaagg agtttcacgc tcacttgcat agctggcaag cgttgtgtca tatcctgcgc
    10561 gatctacata gcggtgtgca actccccggc gtttcgcttt ctgcggcggt agccttgctg
    10621 gagcgacaca gtcaggcgat tcatgtcctg gcactggatc gcggcgcgac tcttggtgcg
    10681 ctgatgcgtc tggaacatcc caacgctagt gccgaagcgg cgctgacgat gctggcgcag
    10741 ttatccccgg cacaatccgg cgaggcgctg cacggtttgc tggcattagc ccgtcatcaa
    10801 ctggcctgtc agccggcatt tatcgccggt ttcagcagtc atttaaatca actaagtgat
    10861 gccgatttta ccaatgccct gcccgattta cgcgcggcaa tggcctggct accgccacga
    10921 gaacgcggga cgctggcgca tcaggtgctt gagcattatc aactggcgca acttcccgtt
    10981 tcggcactgc aaatgccgtt gcattgtcca ccgcaagcca ttgcacatca tcaacaactc
    11041 gaacagcagg cactggcatc gctgcaacac tggggagttt tccatgtctg aactgaacga
    11101 tcttctgacc acccgtgagc tacaacgctg gcgattaatt cttggcgaag cggcagaaac
    11161 gacgctttgt gggctggatg acaacgcccg gcagatagat cacgcgctgg agtggctgta
    11221 tgggcgcgat cctgaacggc tccagcgtgg tgaacgctct agtggattag gtggctcaaa
    11281 tctcaccact cctgagtgga tcaacagtat tcacacgctg tttccgcaac aggtgattga
    11341 gcggctggaa agcgatgccg tactgcgcta cggcattgaa gatgtggtga caaatctcga
    11401 cgtgctggaa cgtatgcagc cttccgaaag cctgctacgc gccgttttgc acaccaaaca
    11461 tctgatgaac cccgaagtac tggctgccgc ccgcaggatt gtgcgccagg ttgtggaaga
    11521 aattatggcg cgactggcaa aggaagttcg tcaggctttt tctggtgtcc gcgatcgtcg
    11581 tcgccgctca tttattccac tggcgcgaaa ctttgatttc aaaagtactc tgcgcgccaa
    11641 cctgcaacac tggcacccgc aacacggcaa gttgtatatc gaattccccc gctttaacag
    11701 ccgcattaaa cgccaaagcg agcaatggca actggtctta ctggttgatc aaagcggatc
    11761 gatggtcgat tcggtgatcc actctgcggt gatggcggcc tgtttatggc agttacccgg
    11821 cattcgtacc catctggtgg cgtttgacac aagcgtcgtt gatctcacgg cagacgttgc
    11881 cgatccggta gagttattaa tgaaagtgca attaggcggc ggaaccaata tcgccagtgc
    11941 catggagtat ggtcggcaac ttattgaaca acctgcaaaa agcgtcatta tcctcgtgag
    12001 cgatttttat gaagggggtt catcatcatt gctgacgcac caagtgaaaa agtgtgtcca
    12061 gagcggcatc aaagtgctgg gactggcagc gctcgatagc acagcaacac cttgctatga
    12121 ccgcgatacg gcccaggcgc tgattaatgt cggcgcacaa atagccgcca tgacaccggg
    12181 cgagctggca tcatggcttg cggagaatct tcagtcatga attcactacg tccggaatta
    12241 ttagaactga caccgcaggc actgacggcg ttaagcaatg ccggttttgt taagcgcagc
    12301 cttaaggaac tggaaaatgg caacgtcccg gaaatcagcc atgagaacag cgctttaatc
    12361 gccaccttca gtgacggtgt ccgtacccag ctggcgaacg gccaggcact gaaagaggct
    12421 cagtgcacct atggggccag cggcatgtgc cgtcatcgcg tgatgctggt gttaagttat
    12481 caacgacttt gtgctaccgc ccagcctacg ggaaaagaag aggaatggga tccggcaatc
    12541 tggctggaag aactggctac ccttcccgat gccacccgca aacgcgcaca ggcgctggtc
    12601 gctaaaagca tcaccattga gttgttctgt gcgccgggtg aaattccctc tgcccgctta
    12661 ccgatgagcg atgtgcgttt ttattcccgc agcagtattc gtttcgcccg ttgtgattgt
    12721 attgaaggca cactttgcga acatgtcgta ctggcagtac aggcctttgt ccaggcaaaa
    12781 gcgcagcaag cggaattgac tcatttaatc tggcagatgc gcagcgaaca cgtcacatca
    12841 tctgacgatc cgtttgccag cgaagaaggc aaaacgtgtc gtcaatatgt tcaacaacta
    12901 agccaggcat tatggctgag cggcatcagc cagccgctta ttcactacga ggccgctttc
    12961 agccgcgcgc agcaagcagc ggaacgctgc agctggcgat gggtgagtga atcgctacgg
    13021 caactgcgcg ccagcgttga tgccttccac gcccgcgcca gccactatca tgccggagaa
    13081 tgcttacgtc agcttgcggc attaaacagt cgattaaatt gcgcacaaga gatggcccgg
    13141 agcgacagtg ttggtgaagt tcctcctgtg ccgtggcgca cggtcgttgg ctctggcatt
    13201 gccggagaag caaagcttga tcatctgcgg ctggtgtctt taggtatgcg ttgctggcag
    13261 gatattgagc attatggttt acgcatctgg tttaccgatc ccgacaccgg cagtattttg
    13321 cacctttcgc gcagttggcc gcgaagtgaa caggaagact caccggcagc tacgcgtcgg
    13381 ctgtttagtt ttcaggctgg cgcactggcg ggtgggcaaa ttgtttcaca agcagcaaaa
    13441 cgcagtgccg atggcgagct gctgttagct acccgcaacc gcttaagcag cgttgtaccg
    13501 ctgtcgcctg atgcctggca aatgttgagc gcgccgttac gccagccggg cattgtggct
    13561 ttgcgggaat atttacgcca gcgtcccccc gcctgcatac ggcctcttaa tcaggtcgat
    13621 aacttattta ttctgccggt cgctgagtgt atttcgctcg gttgggatag cagccgccag
    13681 acgctggatg cgcaggtaat cagcggcgaa ggtgaagata atctgctgac gttatcactg
    13741 ccagcctcag ccagcgcgcc ttatgccatt gaacgcatgg cggcgctttt gcaacaaaca
    13801 gacgaccccg tgtgtctggt ttctggcttt gtcagttttg ttgatgggca attgacactg
    13861 gaaccacagg taatgatgac aaagactcgt gcctgggcgc tggacgcaga aactgcgcct
    13921 gtggtggtat cactaccttc tgccagcgtt ctgcctgtgc cgtctactgc tcatcagttg
    13981 ctgatgcgct gccaggcgtt acttattcaa ctgctccata acggctggcg ctatcaggaa
    14041 cagagcgcta ttaatcaggc agagttgctg gcgaatgacc tcaccgcggt gggtttttat
    14101 cggctggcac atgtgttggc acaatttcgt aatacagaaa gcgaggcacg ggtagaagca
    14161 atgaataacg gtgttttgct ttgcgaacaa ttattcccca tgcttcagca acaaggatga
    14221 aatagtgctt tttactaaga gttctactcc agttccggac tgctcacgcc acggtattag
    14281 gcatatccta tatagcccct ggtgagagtc acc
//
LOCUS       DAJTRB010000099        13956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTRB010000099 DAJTRB010000000
VERSION     DAJTRB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.099"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="OO330_003304"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="OO330_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5884062.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="OO330_003305"
     CDS             complement(1604..1777)
                     /locus_tag="OO330_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5884063.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="OO330_003306"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="OO330_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5884064.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALSRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="OO330_003307"
     CDS             3415..3744
                     /locus_tag="OO330_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5884065.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OO330_003308"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OO330_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5884066.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OO330_003309"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OO330_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5884067.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OO330_003310"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OO330_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5884068.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OO330_003311"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OO330_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5884069.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OO330_003312"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OO330_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5884070.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OO330_003313"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OO330_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5884071.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="OO330_003314"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="OO330_003314"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5884072.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3454 c         3249 g         3595 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aagccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggtta accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgagcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga caacgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtataacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaagct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacggg gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DAJTRB010000100        13954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTRB010000100 DAJTRB010000000
VERSION     DAJTRB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.100"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            192..2039
                     /gene="ldtD"
                     /locus_tag="OO330_003315"
     CDS             192..2039
                     /gene="ldtD"
                     /locus_tag="OO330_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5884073.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2220..2768
                     /locus_tag="OO330_003316"
     CDS             2220..2768
                     /locus_tag="OO330_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5884074.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2795..3442
                     /gene="gloC"
                     /locus_tag="OO330_003317"
     CDS             2795..3442
                     /gene="gloC"
                     /locus_tag="OO330_003317"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5884075.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO330_003318"
     CDS             complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO330_003318"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5884076.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO330_003319"
     CDS             complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO330_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5884077.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO330_003320"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO330_003320"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5884078.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO330_003321"
     CDS             complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="OO330_003321"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5884079.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9766..12381
                     /gene="pepN"
                     /locus_tag="OO330_003322"
     CDS             9766..12381
                     /gene="pepN"
                     /locus_tag="OO330_003322"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5884080.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OO330_003323"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="OO330_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5884081.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..>13954)
                     /gene="ssuC"
                     /locus_tag="OO330_003324"
     CDS             complement(13352..>13954)
                     /gene="ssuC"
                     /locus_tag="OO330_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5884082.1"
                     /translation="ISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQMLRNV
                     PHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYGLSG
                     IPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQTDV
                     VVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3560 c         3522 g         3321 t
ORIGIN      
        1 ataacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac gtatcggcgt
       61 ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt tcgtgagtat
      121 tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat gataaaaaca
      181 gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca atcagtttgt
      241 gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct gaagtaatcc
      301 ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag gcgcaagcca
      361 cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa gcccgcacgc
      421 aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag cttcaactgt
      481 tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt aaagccttcc
      541 agcaacagct ggcagaggtg gcgattgctg gtttccagcc gcagtttaat aaatgggtag
      601 agttactgac cgatcctggt gttaacggga tggcacgtga cgtggtgctc tctgatgcga
      661 tgatgggcta tctccatttc attgcaaata ttccgatcaa aggcactcgc tggctatata
      721 gcagtaaacc ttatgcgctt tcaacgccgc cgctttcggt gattaaccaa tggcagctag
      781 cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag catccgcaat
      841 atgcggcgat gcatgaatcg ttactggcct tactcagtga caccaaaccg tggccccaac
      901 tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg gcgttgcgcg
      961 aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta cctggcgatg
     1021 acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca gcagaaacta
     1081 agccgatgga caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt cgtgccgcct
     1141 acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga ttgggagcag
     1201 atggtgctat tggcccggca acgcgtgact ggttaaacgt aacgcccgcc cagcgtgctg
     1261 gcgtgttggc actcaacatc cagcgattgc gcttgctgcc aacagagctt tctaccggga
     1321 tcatggttaa cattccagcc tattcgctgc tctactatca gaacggcaat caggtgctgg
     1381 attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc agtgcactta
     1441 acaacgtagt ggtaaacccg ccgtggaacg taccaccaac tctggcacgc aaagatattc
     1501 tgccgaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg gtgatgcgcg
     1561 gctggaacag cagagaagcg attgacccat gggaggttga ctggtctaca atcacggcct
     1621 cgaatttacc gtttcgcttc cagcaggctc caggcccacg gaactcgctg gggcgctata
     1681 aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac cacaatctgt
     1741 tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa gcttccgatc
     1801 tggcgaatat gctgttgcag gatgcaggat ggaatgacaa acgtatttct gatgcgctga
     1861 agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat ctctactacc
     1921 tgacggcctt tgttggtgca gatgatcgta cccagtatcg tacagatatt tacaattatg
     1981 atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta atcaggtaaa
     2041 tgaagtagtt cggggaaatc aattgtcgta ataattagca tgattggggg cgattctctg
     2101 cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg cggttaaggt
     2161 gcctcttgtg cgccagaagt gcatataaac gataacattg agctgtagac ttgattatca
     2221 tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt gcactcggtg
     2281 ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg cgcattttga
     2341 cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat ggcagaggct
     2401 atattcagga agaattggca aaacttaacc attttttccg cgattaccgc gcgaacaaaa
     2461 taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg ttgttaggca
     2521 cgcgcaaacc ggtgcaactc atttccggtt atcgttccat tgataccaac aatgaactac
     2581 gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag gcgatggatt
     2641 tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct atgcgcgcag
     2701 gtggtgtagg atattaccca cgtagtaact ttgtgcatat tgataccggg ccagcacggc
     2761 actggtagta atcgcttaac gaaacagggg cagtatgaac tatcgtatta ttccggtcac
     2821 cgcattctcc cagaactgtt cattgatctg gtgtgaacaa acccgcctgg ccgcactggt
     2881 cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg gcctgacact
     2941 gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg cggaactggc
     3001 gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct ggctgcaagg
     3061 cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga cgccagatcg
     3121 ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg tgttacattg
     3181 ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc tgctgatttc
     3241 tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg gcgatcataa
     3301 tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg tgacatttat
     3361 tccgggtcac ggaccattat ctacacttgg ttatgagcgc ctgcacaatc ccttcctgca
     3421 agacgaaatg cccgtctggt aaaggcgcat aaaaaagtcc acttttaatg cgggcctgga
     3481 tttctggcaa agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac
     3541 aaaatcgtgc aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc
     3601 aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta
     3661 acgcttacag cactgccaca atcgattcgc acagcggagc catgttatct ggtgtcatcc
     3721 cggccacatt tacgcgacca gaagcaacag catatacgcc aaactcttcg cgcagacgca
     3781 gcacttgttc ttttgtcaga ccactgaagg agaacatgcc gttctgtttg atgataaagc
     3841 tgaagtcgcg gtttgcacct ttttcctgca gcgtattgac gaacaactga cgcatacgct
     3901 gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaat gcatcgttgc
     3961 tcaggatggt ggcaacaaca gaagcgccgt gtgctggagg gttagagtag ttagcgcgaa
     4021 tcgccgcttt catttggctg aatgcgcgat caacgatttc actgtcggca gcaaccagag
     4081 tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa
     4141 tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct tccagaccac
     4201 gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt
     4261 gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc
     4321 catggaacag cactacgtcg ccagcctgag cttcgttcag actgttaatc agtgcatcga
     4381 agtcaagggt gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag
     4441 agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacaca cgcttaacgc
     4501 tggtattttt tgccaggaag tcggcagcca cgcgtagtgc gccagtgcca cccggagtct
     4561 gtgccgtgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct
     4621 gagtgcagcg accaaattca aggatgccgt caatgccgag gtaatttttg gtggtttcat
     4681 tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct
     4741 catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca
     4801 gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga
     4861 ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat
     4921 tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag
     4981 gcataaaaaa aacaggacca acgtcctgtt ttttcggcat ttaacaaaga ggtgtgctat
     5041 tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgttg
     5101 tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag
     5161 taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct
     5221 ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc
     5281 gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta
     5341 gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg
     5401 tcgtacttca gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct
     5461 tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat
     5521 tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg
     5581 tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag
     5641 ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta
     5701 tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac cacaccgtag
     5761 ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc
     5821 gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccctggaa gttatattcc
     5881 cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg
     5941 gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa
     6001 tgcagaccaa cagctttacc gtacagatct actttgttgc catctttgtt atagatttct
     6061 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
     6121 ttcatcatta tttattaccc tcatggtttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa agggagccga
     8281 ttagctccct gatgatatta actggctttt ttaatatgcg gaaggtcgaa cgctttgcgc
     8341 agcgcccgaa caaacgcttt atcatggcag atagttttgc cagggctgtc agaaagtttc
     8401 gccaccggtt taccgttaca ctctaccaac ttaatcacaa tattcagggg ttttacctgg
     8461 gggatatcgc aggtcaggcg agtcccaata ccaaaactta attgcacgcg ggaagagaag
     8521 tggcggtata gctcaaccgc tttgcgtaaa tccagattgt cagagaaaac cagcgtttta
     8581 ctctgtggat caattcccag cttttcataa tgtgcaatgg ctttttcacc ccattcaacc
     8641 gggtcgccag agtcatgacg caggccctga taccgactag cgaactcgac accgaaatca
     8701 cgcaggaaag catccatagt gatgcagtcg gttaatgcaa tgccaagttg gtcgggatac
     8761 tcttccagcc aggcagcaag tgcagctcgc tggctgttgg ctagatccgg gctgatttgc
     8821 tgatgtgcct ggaaccattc gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc
     8881 gccagatcgt agttgctggt gcccacgaac caggattcct gttgcagacg cttaacgatg
     8941 gtttcttgta cttcgcgaga aaaacggcga cgggtgccaa aatccatcag atggaagcgc
     9001 gacatatcaa gaccggcggt taacgccgag aagtcgacta atttgctttc cagcgtgtcg
     9061 agggcttgcg caacgtcggc ctgcggtgag cgatagcgat gtaccatttc actgataacc
     9121 gccagcaaag gaacttccca gaggatgact tcacgccacg gaccgcttaa gcgaatatcc
     9181 agcttgccat tatcgttgga cacggtgact tgttccgggt taaagcggaa ctcgcgtaac
     9241 cagttaagat agtcggcctt aaagaaaggc agggcagaga gccactgata ttcatcatcc
     9301 tgcaggcgca ggtgctgcat cgcctgaatc tgttcacgaa tagcatcggc ataaataccc
     9361 agcagatcgt cacctcggca acgaaactcc gccgcgacat gcacatcgta atagtgatga
     9421 aacacggctt gctgcatatg caacttataa gcatctgtat ccagcaacga gtgcagaaca
     9481 ggagaagcga attgtgtcat aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca
     9541 ataaacatct tcaggaactg ctggagtata ccttgtttcg caatttattg aaccccgatc
     9601 acaccatatg ccacctttct ggtcgatggc attacgcggt gtatgttata aaaatgtagc
     9661 aataaaggcg tttgtacctg aaaagatgaa gattctgcat agcgcgattt acgcaacagg
     9721 aatagactga acaccagact ctataaaaga tgctaaaggt tatttatgac tcaacagcca
     9781 caagccaaat accgtcacga ttatcgtgcg ccggattacc agattactga tattgacttg
     9841 acctttgacc tcgacgcgca aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat
     9901 ggtgcatcag atgctccgct tcgtctcaac ggcgaagacc tcaaactggt ttctgttcat
     9961 attaatgatg agccgtggac cgcctggaaa gaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcgttttac gcttaagatc attaatgaaa taagcccggc ggcgaatacg
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaatcaa atatcccttc ctgctttcca atggtaaccg cgttgcgcaa
    10261 ggcgaactcg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgatacctt taccacgcgt
    10381 tctggtcgcg aagtagcact ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactccatg aaatgggatg aagaacgctt tggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccggttg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggt aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagcg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catagacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg taatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgcc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggtggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct ttacgagaag
    12361 ataactaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ctaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctgccg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actgaccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtatcatcct gaatttcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac cggccagcag gtgcagcagg
    13201 gtacttttac caccaccgct gcggcccacc accgctacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcatatcgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc tgtttgcaaa aactctcgcg
    13501 cattcatcgc cagataacca atgccggaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcatcgg
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatctcca
    13681 ccagcccgcg atcgatatta cggatgccat gccaggtgtt gatataaatg gggaacagcg
    13741 taccgagcgc caccagaaag attttcgcgg attcatcaat gccaaaccac aaaatcacca
    13801 gcgggatcag cgccagatgc ggcacgttgc gcaacatctg aattgaggta tccagcagcc
    13861 tttctcccca gcgcgacagt ccgctaatca gtcccagaat caggcccagc gatccgccaa
    13921 ttgaaaagcc aatcagcgcc cgccaggaac tgat
//
LOCUS       DAJTRB010000101        13793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTRB010000101 DAJTRB010000000
VERSION     DAJTRB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.101"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO330_003325"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OO330_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5884083.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO330_003326"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OO330_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5884084.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTSGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO330_003327"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OO330_003327"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5884085.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO330_003328"
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OO330_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR5884086.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO330_003329"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OO330_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5884087.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO330_003330"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OO330_003330"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5884088.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO330_003331"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OO330_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5884089.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="OO330_003332"
     CDS             7490..7639
                     /locus_tag="OO330_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884090.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="OO330_003333"
     CDS             7840..9423
                     /locus_tag="OO330_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5884091.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO330_003334"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OO330_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5884092.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO330_003335"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OO330_003335"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5884093.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO330_003336"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OO330_003336"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5884094.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..>13793
                     /locus_tag="OO330_003337"
     CDS             13382..>13793
                     /locus_tag="OO330_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR5884095.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPL"
BASE COUNT         3196 a         3640 c         3487 g         3470 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gactcgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc gatgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctggca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgagtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gagagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct agccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccaac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 catcctgctt ttttccacca gctcgctgaa catgacctgg acgaccatca atattgttga
    13681 agccgtggtc ggggcagtgc tgctacgtaa attgctgccg tggtataacc ccttgcaaaa
    13741 tctggctgac tggctgcgtc tggcattcgg cagcgccatt gttccgcctc tgt
//
LOCUS       DAJTRB010000102        13533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTRB010000102 DAJTRB010000000
VERSION     DAJTRB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.102"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..896)
                     /locus_tag="OO330_003338"
     CDS             complement(<1..896)
                     /locus_tag="OO330_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5884096.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGG"
     gene            1137..1937
                     /locus_tag="OO330_003339"
     CDS             1137..1937
                     /locus_tag="OO330_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5884097.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2015..2785
                     /locus_tag="OO330_003340"
     CDS             2015..2785
                     /locus_tag="OO330_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5884098.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2833..4191)
                     /gene="mltD"
                     /locus_tag="OO330_003341"
     CDS             complement(2833..4191)
                     /gene="mltD"
                     /locus_tag="OO330_003341"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5884099.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4263..5018)
                     /gene="gloB"
                     /locus_tag="OO330_003342"
     CDS             complement(4263..5018)
                     /gene="gloB"
                     /locus_tag="OO330_003342"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5884100.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5052..5774
                     /locus_tag="OO330_003343"
     CDS             5052..5774
                     /locus_tag="OO330_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5884101.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5771..6238)
                     /gene="rnhA"
                     /locus_tag="OO330_003344"
     CDS             complement(5771..6238)
                     /gene="rnhA"
                     /locus_tag="OO330_003344"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5884102.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6303..7034
                     /gene="dnaQ"
                     /locus_tag="OO330_003345"
     CDS             6303..7034
                     /gene="dnaQ"
                     /locus_tag="OO330_003345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5884103.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7167..7243
                     /locus_tag="OO330_003346"
     tRNA            7167..7243
                     /locus_tag="OO330_003346"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7201..7203,aa:Asp,seq:gtc)
     gene            complement(7374..8420)
                     /locus_tag="OO330_003347"
     CDS             complement(7374..8420)
                     /locus_tag="OO330_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5884104.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIHDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8431..9416)
                     /gene="tssG"
                     /locus_tag="OO330_003348"
                     /pseudo
     CDS             complement(8431..9416)
                     /gene="tssG"
                     /locus_tag="OO330_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9413..11296)
                     /gene="tssF"
                     /locus_tag="OO330_003349"
     CDS             complement(9413..11296)
                     /gene="tssF"
                     /locus_tag="OO330_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5884105.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEDESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11312..11806)
                     /gene="tssE"
                     /locus_tag="OO330_003350"
     CDS             complement(11312..11806)
                     /gene="tssE"
                     /locus_tag="OO330_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5884106.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11803..12633)
                     /locus_tag="OO330_003351"
                     /pseudo
     CDS             complement(11803..12633)
                     /locus_tag="OO330_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12620..13522)
                     /gene="tagK"
                     /locus_tag="OO330_003352"
                     /pseudo
     CDS             complement(12620..13522)
                     /gene="tagK"
                     /locus_tag="OO330_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3103 a         3524 c         3608 g         3298 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttctccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      421 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      481 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      541 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      601 gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      661 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      721 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      781 ttcaccgccc ggcttaccgc tgagtttgct tgccctaatt gttccgctgc ccggctaaag
      841 ctgccgcttt cgacgaccga aacaaaaatg gcgagttctt ccgacgtggc tttcattttt
      901 gctcctgttg caaaatagat gagatatttt gaatttattt gtcattaaac catcaggatg
      961 cgtgatatgt catccgattt aatgttctca ataatgagca aatttctgac aggtgtaagc
     1021 acttgcttac ataacaatat acaattgctc gttgaaagag tgagctaaaa tccctataac
     1081 agtagaaccc tcccgagtgc ggaagggttg acgtaataga ggtttcaaag tcaaaagtgc
     1141 gaaaaaacac ctatgccatg cgctatgttg ccggacaacc tgcggaaagg atcttaccgc
     1201 cggggtcttt tgcgagcatc ggccaggcat taccacctgg agaaccgtta agtaccgaag
     1261 agcgtattcg gatcctggtg tggaacatat acaaacagca acgcgctgaa tggttgtcgg
     1321 tattaaagaa ctacggcaaa gatgcacatc tggtgttatt gcaggaagcg cagacaacgc
     1381 cagagttagt acagtttgcg accgctaact atcttgccgc cgatcaggtg cccgctttcg
     1441 tgctgccaca acatccttcc ggtgtaatga ccctttcggc ggcgcatcca gtgtattgct
     1501 gcccgttacg tgaacgagaa cccattttgc gtctggcgaa gtcggcactg gtgacggtct
     1561 atccattacc tgacacccgc ctgttgatgg tggttaatat acacgccgtc aacttcagtc
     1621 tgggcgtgga tgtctatagt aagcagttac ttcctattgg tgatcagata gctcaccaca
     1681 gcggcccggt cattatggcg ggagatttca atgcctggag ccgtagaagg atgaacgcgt
     1741 tatatcgctt tgcgcgggaa atgtcgctgc gccaggtgcg ttttaccgat gatcagcgcc
     1801 gtcgggcgtt tggccgcccg ctcgattttg ttttctaccg tggtctgaac gtcagcgaag
     1861 cttctgtatt ggttacgcgc gcttccgatc acaatccgct actcgttgaa ttcagtcccg
     1921 gcaagcctga taaataaggt atgtcaggtc tgccacaggg cagaccaacg tttggcgctg
     1981 tccttttata ctcaaacaac aaaaggacag cgcaatgaca acacaatccc accatgacca
     2041 cgtagaaaag cagtttagct ctcaagcctg cgaatatctg accagtaccg tacatgcatc
     2101 cggacgagat ttacagcgcc tggcggtgcg tctggctgat tatcctggtg caagtgcact
     2161 tgatatgggc tgcggagcag ggcatgccag ctttgttgct gcgcaaaacg tgagcgcggt
     2221 ggtggcgtat gacttatctg cccaaatgct ggatgtcgtg gcacaagctg ccgaagcccg
     2281 gcaactgaaa aatatcacca cccgccaggg atatgccgaa agtctgccat ttgccgataa
     2341 cgcatttgat attgttatca gccgttattc tgcccatcac tggcatgatg ttggtgcagc
     2401 actgcgtgaa gtgaaccgga tattgaaacc tggcggtagg atgattgtga tggacgtaat
     2461 gtctccgggt catccagtgc gcgacatctg gttacagacg gtagaagcat tacgcgatac
     2521 ctctcacgta cgaaactacg ccagcggtga gtggttgagg ttaatcaatg aagccaatct
     2581 gatagttgat aatttaatta cagataagtt accgctggaa ttttgttcat gggtcgcgag
     2641 aatgcgtacg ccagaagcgt tagtagacgc tattcgcatt taccaacaga gcgcatcgac
     2701 agaggtgaga acgtattttg ccttgcagaa tgacggctct ttcaccagtg atatcatcat
     2761 ggtagaagca cataaagcgg cataaataaa aaaggcaccg ggggaatcgg taccttttta
     2821 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttgtc
     2881 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatc acatctttga tgttcacgcc
     2941 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     3001 gctgttgttt gccagtcgct gtgcgctact gcctgcacca atcgtcaaac tttggcctgg
     3061 cttcagctta gatccgcgca gtttgttcca ctgctgcaaa tctttggtgc ttacgccgag
     3121 acgtgaagcg atgcttgaaa gcgtgtcgcc agagcgtacg gtgtaaacac ggctgttaag
     3181 cggcgtattg ttggcaacca gcgtcgactg tacagcagca atttcgcctg aagcgagaga
     3241 ttcacgcagt tgatctgcat gcttctttgg caccatcacg tactgcggcc cacttgcgcc
     3301 cagcgtggag cctttcacgc cagcgttgaa tgtcttcagc ttgctgacgg aaatccccgc
     3361 catatctgca accttcgcca tttcaaccgg gctactcagg tgcacacgcg ccagagcacg
     3421 gctttcatcg gtcgttggca gacgtacgcc ataacgcttg ctgtttttga gaatatcact
     3481 caatgccagc attttaggca cgtactgctt cgtttcctgc ggcaacggta acgaccagaa
     3541 gtccgtggat ttcccacgcg ctttgttcgt tttaattgcc ttcatgaccc gaccttcgcc
     3601 gctgttataa gccgctacgg tcagaagcca gtcgtcgtca aacattttgt tcagacgctg
     3661 catcatattc agcgcggcag ttgttgaagc aacaacatcg cgacgcgcgt cataattgcg
     3721 ggtctgtttc aaaccataat tgcgccccgt gctcggaatg atctgccaga tgcctgcggc
     3781 attggcgcca gacgttgcgt gaggatcaaa agcgctctcc actatgggta gtagtaccag
     3841 ttccataggc atgttacgtt ttttaacttg ccctgctatc cagtacatat acggctctgc
     3901 ccgtaaagtt acatcgtgga gatagctctt attgcgtaaa tatttctgtt tctgttcgcg
     3961 aatccggtca ttttccggaa ttcccatctt tagctcgtcg ccaatgaaag cccacaagtc
     4021 accatctggc gcgatagacg tcccatcgtc catccatcgt gcctgacttg taaactttgc
     4081 tgcttcccct tgaccagctg cagaaaggct ctgtgcgtgc tgttgaacgt tgccggtact
     4141 ctggcaaccc acgagcagga cagaggcgag taatatcgct tttgccttca tgtgtgtgtc
     4201 aatagttgct taaaagacga ccgatcataa cggcgaacgg agccgatgac aagaaagttt
     4261 tatcagaacc tatctttctt tgaccttaac catgcaaaac gctcttcagg ttgttgcaat
     4321 aatgtttctt cattaattcc attaattaaa tcaatatctt ccgttcttaa aaaaacatta
     4381 atttgccgct catttttcag aattacgggt agtgttattt gattttttgc ccgtaactcc
     4441 ttaactttac gataataatc atttatggac aaatcgtgcg gaaaaatact caaagcaaac
     4501 ttcatatttg ataacgtata ttcatgagca caacatacca atgtatcgtc aggtaacgca
     4561 cttagctttt taagtgattg atacatttgt aaagctgtcc cttcaaacaa ccgaccacaa
     4621 ccaccagaaa acagcgtgtc gccgcaaaat agataaggtt tactgaagta acagatatgt
     4681 cctaaagtgt gacccggtgt ggtaattaca ctaaattcat gccctaaaac gaaggcagtt
     4741 tcgccatctt tgactacctg tgttgttccc ttatcttgtg tctcttgtgg accatacacc
     4801 acaatttgcg gaaacttttc caccagttct tttacgccgc caacgtgatc gtggtgatgg
     4861 tgggttagaa atatggcctc cggttgccag ttattggcgg taatggcgtt taatactggc
     4921 tctgcgtctc cgggatcgac aatcaggcag cgacctgcct catcattcaa aacccagatg
     4981 taattgtcat caaaggcggg aatactgtta agattcatag attacctctc agtgtaaaac
     5041 ggaaggttgt gatgaaaccg gcaagagtcc ctcaaactgt cgtggctcct gattgctggg
     5101 gcgatttgcc ctggggagag ctttatcgca aggcgctgga gcgccagctc aacccgtggt
     5161 tcactaaaat gtatggtttt catctactta agattggcaa tttaagcgca gaaatcaatt
     5221 gcgaagcgtg cgcggtttct catcaagtga atgtttctgc gcaaggaatg cccgtccagg
     5281 tgcaggcgga tccacttcat cttccttttg ccgataaatc cgttgatatt tgtctactgg
     5341 cacatacctt gccgtggtgc accgatccgc atcgtttatt acgtgaagcc gatcgggtac
     5401 tgattgatga tggctggctg gtgattagtg gcttcaaccc catcagttta atgggattac
     5461 gcaaacttgt gccggtattg cgcaaaacct cgccctataa tagccggatg tttactctga
     5521 tgcggcaact ggactggctc tctttgttga attttgaagt gctgcacgcc agccgtttcc
     5581 acgttctccc gtggaacaaa cacggaggaa aactattgaa tgcgcatatt cctgcgcttg
     5641 gttgcttaca acttattgtt gcccggaaac ggactattcc tttaacgcta aatccgatga
     5701 aacagagtaa aaacaagcca cgaattcgcc aggcggttgg agccacccgg caatgtcgta
     5761 aaccacaggc ttaaacttcg acttggtagc ccgtatcttc cagtgtggga ttcatcgccg
     5821 cggcacgagc cagttcatca cagcgttcgt tttccgggtg tccggcatgg cctttaaccc
     5881 attcccattt gatttgatgc tgccccaatg cagcatcaag acgttgccag agatcgacat
     5941 tttttactgg ttttttgtct gctgttttcc agccacgttt tttccagtta tggatccact
     6001 gggtgatacc ctggcggaca tactggctgt cggtactcaa aatgacttcg caatgttctt
     6061 ttaacgcctc cagcgcgaca atagcggcca tcaactccat acggttgttg gtggtgcggg
     6121 tgtagcctgc gctaaaggtt ttctcgcgtc cgcgatagcg taaaatagcg ccgtaacccc
     6181 caggtccagg attacccaga cacgaaccat cggtgaaaat ttctacctgt ttaagcatct
     6241 ctggtagact tcctgtaatt gaatcgaact gtaaaacgac aagtctgaca taaatgaccg
     6301 ctatgagcac tgcaattaca cgccagatcg ttctcgatac cgaaaccacc ggtatgaacc
     6361 agattggtgc acactatgaa ggccacaaga tcattgagat tggtgctgtt gaagtagtga
     6421 accgtcgcct gacgggcaat aacttccatg tttatctcaa acccgatcgg ctggtggatc
     6481 cggaagcctt tggcgtacat ggtattgctg atgaattttt gctcgataag cccacgtttg
     6541 ccgaagtagc cgatgagttt atggactata tccgcggcgc ggagttggtg atccataacg
     6601 cagcgttcga tataggcttt atggactacg agttttcgtt gcttaagcgc gatattccga
     6661 agaccaatac cttctgtaag gtcaccgata gccttgcggt ggcgaggaaa atgtttcccg
     6721 gtaaacgcaa cagcctcgat gcgctatgtg cccgctacga aatagataac agtaaacgaa
     6781 cgctgcacgg ggcattactc gatgcccaga tccttgcgga agtttatctg gcgatgaccg
     6841 gtggtcaaac gtcgatggct tttgcgatgg aaggagagac acagcagcaa caaggtgaag
     6901 caacaattca gcgcattgta cgtcaggcaa gtaagttacg cgttgttttt gcgacagatg
     6961 aagagctggc agcacatgaa gcccgtctcg atctggtgca gaagaaaggc ggaagctgcc
     7021 tctggcgggc ataaatacat gtaaaaggcg ctaaaaatag cgacttgggc gatttttgca
     7081 gcaaacgatt caaaagatga gaaaaatcgt tgacgaaggt cgaggcaatc cgtaatattc
     7141 gcctcgttcc caacggaaca caacgcggag cggtagttca gtcggttaga atacctgcct
     7201 gtcacgcagg gggtcgcggg ttcgagtccc gtccgttccg ccaatattca gaaagcccga
     7261 atcagtaatg attcgggctt tcgtcgtttt ggtgaagtga gaatttttca ctccgaagta
     7321 aaacggatat gatttaaaaa atggccagac attataaatt ctggccactt ctgttactcc
     7381 agcgcatccc atttctccag cagttctact ggaatttcat ttattacctc cgagacgcgt
     7441 tttccgacca ggcgttctgc ctggcgcagc aaaggaatgg tcgggctgct cggttcgctg
     7501 tgctcaaacc agcgacggat gacacgcagg cgttccagcg cgtcgtttcg gtcgtggatc
     7561 tgccggggtt ctgcgccggt catcggtgat atttcctccg cgatatgcac cggtagcgca
     7621 atctgctctg cctgcatctg ttcagtatga acgatggtgg cgttttccgg ctgctcagcc
     7681 tgtggttgtg gcaggatctc cggcgtggtg ctctgcactg ctcccggtaa cagggccagc
     7741 agttgcgtca gacgggagaa gtccggtgcc tggtcattca gcgtttcacg cgcatggcgt
     7801 tgcagccgct cagtggtctc tgctgcctgg cgaaaggcat ccagcggcag tgtgccgcgc
     7861 gcttccaggt ctgccagttg ctggcgtacc gattctggtg ccagcgcatc cgccgggcgt
     7921 aatgcagata gcgaacgctc cacatcccgc acctgaaggc gcatggcagc attatttgac
     7981 agtgtaatgc cacgaatatc cgccattaca ccttcgtggt ccagcaacgc cgccagcgcg
     8041 ttactgcgtg ccaccgcagc atcttcggcg gtgatatctt cagtcgcgag cagttgagga
     8101 tggatggcgt ccgggtacgt ggagcacaac gtttccagca gcgtgagcgc ctccgacagt
     8161 cccctggcac cggcctgctg gatacggcag cgcagcagga caaccagaat gcggatatct
     8221 ttgctgcgca tcagcagacg atgggcgtcc cgctcaagct ccgcccagtt aatgttttcc
     8281 ggcgtactga caaaatcgcc atattgcgct tcttcacgcg gtgcggcgcg ggtgaacagc
     8341 agcaggtatt ccggatcgta ctccagatcc gggccgcagg ggcattccgc cgaaattggg
     8401 tcagacagga atatttccat ttaattatcc tcagtgcgcc aggtactgtt ctggttcgaa
     8461 aatcatgccc gtcaccgggt tgtcgtgcat ggctttaccc gcccatgcag tccaacccag
     8521 ttggtacgga ccaccggtaa cagccggagg ggcggcgtgg ggctttagct gtagctcaat
     8581 ttcccagcag tattcataac cactaaaggc gcggaccagt tctgtcagga cgggcaggtt
     8641 attgctgcca ggcagaaaac gtcggtaatc ctccagcgtc agcgggccga taaccagccg
     8701 gaacttatac tgcatgtccg gcacggcctg gccgatgagt gcaccattgc caataacgga
     8761 agagggggca ggcatcccca gccgggtgat ctcatcgtcc gccacggcta tccagtgcag
     8821 gacatactcc tgtatccgga aaggtacacc gaaatagtgc gccagcgtgg tggccagacc
     8881 gtccggattg cgggactcac gcaccagatg ggccgatgcc gccagacgag cgtgatctgg
     8941 caacgggctg tcagcgctct cccgcaaatc ctgcccgctg aggctggcga cataaaaggc
     9001 aaagcggtca tgttgcggtt tatccagacc gcccccagcg gactgggcgc tgcgccatgc
     9061 ctggtaaaac tgcgtcagcc agcggtgatg aaaaagatca gaaaaacggg tcagcgtcgg
     9121 ctttcagtcc ggttacgcgc cagttcggtg tagtgcagcg gcagtggacc gttcggcccc
     9181 cacagcccga ggctgtacag actgatgtgc aggcgccctt cctgccagcc cacactggcg
     9241 atttctcgtg gcgcaaaggt cagcgcgggc gtttgtccga ggcgaaattt ttccatccgt
     9301 ggttgccagg tgttgcccgc cggaacggta cacaactgca catccacgcg gcgcatcagg
     9361 ttcagaaaac cgtaacgcca ggggggggcc tgcgcctgag tgaaatcgtt gctcatacgc
     9421 ttccccgctg tcccgccctg acaggccagg tcataatttt gccgcgctgc atgctgtgca
     9481 gcgtcatctg cgagaaggta ttaatggaaa catggcgggc gatatagtgt tccagcacca
     9541 ggccgaacag atacggactg atgccggaaa aaccttcttc atccaccgtc agttcacaac
     9601 tgacgccacg cccatagacc agtagtccgg aaccgggcag acggcgggtg accggggtcg
     9661 ttttacaacc aatcaggctg cgcacctggc gcgattgcgg actgtcatga gcaggaataa
     9721 acaggttcag cagatctcgc aacgcctggc cgccggtacg gtgatccaga tcggccagcg
     9781 gcagataatt aaaagataac tggcggatca accgccatgc catctcacgt tcagccagcg
     9841 ggggctgcgg cgagcgtggc gggcggataa gccctacgcc cgccaccggg atggctgcat
     9901 ccacggttag atcgtcccgg ccattgcggg caataagaca gggcagatcg cggttagtga
     9961 ccatggccgt gatggtgatg tggcgcagat tttccgggta cggcgcttca tactggtcaa
    10021 ccagcgacag gaacacttcc gagcccgtat agggggtacg ggtgccgtaa cggcgggcgt
    10081 tttctgataa gcggcggggc tcccggcgca gggaaaaata acgtccgtga ttgccttcat
    10141 cattattgcg ggtgtggtag agcgggcgaa aggccatctt ccgtgtggtg tcggtttcca
    10201 gcccttccac ttcctggacc gagaacacct cataatccag cggatgggtt ctgtccacca
    10261 ccagatgctg ttccgtcgtg ctgtgcgtga catcgatccg ggcggtgacg cggggaaaca
    10321 ggttgataac cggggtacag aacaggctga actgtgccgc atttgtctgg tggatcagcc
    10381 agtccggcgg caggcggttc agcaggataa caatctccgc cacgccaccg tcaattttct
    10441 gtaatccggc agacaggcct gtcggggtga aaaaatagaa gcgttccgga caggcaaaat
    10501 attcatgcag caggttatga ccgtgaaaca cgttccacgc cagcggcagc agcccctgat
    10561 ccggctccag gccttcgtac ataacgggct gttgcagatt cacatccagc gcgccgtcaa
    10621 aatgccccgg aatgccagcc agggtcgcga tggcgctggt atgtagaagt tctaacaggt
    10681 gtgaggccgt acgctcctcg ccgcagagat aaaacggcag cctgtccagt ccggcaagct
    10741 gactgaacgt tagttcacca aaggtgcgca gggtaatacg cagcgccccg gcgacgtgaa
    10801 tgttggccgg caggtagcgg tgcagggcag gcatatccgg tggcgcggcg gtcagtcgcg
    10861 cctcttcgat ggcgagcggc cacagcgtta catcctggct gctgcgaaac tggcaggcag
    10921 tgctttcgtc ttccggaatg gcggagaaaa aggctgtgtc gcgcgggacg gtaaatcctt
    10981 tcgccagatc gccttcttcc gtatcgggat gcaactgcgc cacgctcatt gagggcgtgg
    11041 gtgtgacgta attggggctg accacttcca gcaggcgctg ggtgaaacgg gggaattcag
    11101 cgtcaatttt tagctgcgtg cgggcggtca ggaagctgaa ggcctcgacc attcgctcta
    11161 catacggatc ggcaatatcc gttccctgca tgcccagccg ggcggcgact ttgggatgac
    11221 gggcggcaaa ttcggctccc gtttcccgca gatagctcag ttcgcggttg tagtattcca
    11281 gtagccgtgg atccatcggt ctctctctga attaaaaaaa cgtaatgcga ctctgctcca
    11341 tatccagcgc gctacgtacc cggaatgcag tcggatacgg ctgcgtgagg atctcgccgc
    11401 gaatttcaaa ctgtaatgtg ttatggccac tctggcggcg ctcatcctga agtgggatga
    11461 tttgcagagt ggacgctttc agccggggtt caaaccgggt gatggttcgc aaaatggccc
    11521 tgcggatatc gtcccacttg tgctcataca gaaagctgcc cgccagtggc ggcaggccgt
    11581 agttaagtac ggacgctgcc gcgtacggat aacgtgcagc atcaatatca tcttcatggc
    11641 tgacggtgtt gagcagaaat gacaaatccc ggcggatgat ctccttaatc tgaaccggac
    11701 tgacgctgat accgtggtcg cgtttctggt ttggggcgtc gtcacacagg cggtcaaaca
    11761 gcgtgggcag caggtgattg cctggacgct ggcgggaagc gctcacgcgt cgcttccttc
    11821 agcggtgtta aaggtacagg tggacaaatc cagcaggctg atatcgccgt gaccggtgag
    11881 ccacaccctc tgacccagcg cctgtaccag tgtctctccc ggaccgtcct gccagaccgt
    11941 ttcccggcac agacgcaggg agtcggcagc cttttcagag ccgctgtagc ggggaaacag
    12001 ctaggcactg tgtgtatcgc cattgttcag ggtgacgttg acgggtttcc agatcaaatc
    12061 gatcagatgc gcaggccgcg gcgatttcag cgaacagatt tgtgaaaacg gcagccagat
    12121 atatgctccg cccgcgatca gttcgagtac cggaccggta cgggagtcgc tgtcgctaat
    12181 ccagtcaaat gcgccgccat tccactgccc ggaagtgtct gttatggcct caagcgcgcg
    12241 gctgcggtga gcatcagctt cctgcgcttc accacgggcg ttacaggcga gggcggtcag
    12301 cagatcctcc atccaggcgg gcggcgggag aataacaccg ggccgctgtt cgccctgaaa
    12361 gcaggcgtgg cggtatatct cacagcggat cagctcgcca aatacctgcg cttcgcgagt
    12421 ataatttgcg tccatccggg cgcatagctg gatctgttgc agtgcccgcg accagtcgcc
    12481 tgccacgcac aataactgaa acaggctgtg gcggcgggag gcctgcgctg ggtgcatcct
    12541 gacctgttgc tctgccatct gaatgccctc caccacggaa taatcccgca gcagcgccga
    12601 caaggtaatc ggtagctcgt tattgtttgt catgatatat ctctccatgt tgtgccgggt
    12661 ttatccggta atggctgtca atgccaatga tgcgatgctc tttccgtgtc agatctggca
    12721 gtacggtgtt atgcgctgtt tccggggcgt attccgggga aagtaaatgc aggatatcag
    12781 ggggagtctt ttcgacaggc cagatagttt catcttcgcc agtggcatcc agtacattga
    12841 gaatagcgtc aatgcttaac ccaccagtga ccatatcctg caatgtctcg gcttcggtga
    12901 ccaggtcacg caatgaatcc agatcctgcg aggagggctc ttcgttaaaa ggaaaaggat
    12961 cagcatggcg attaagatct tgttcgggag ataacaacgc ctcgcggtat tcctgataaa
    13021 gtcgggtcat ggcatttcct ttttccgcac cttctccggt ggaggaggct ccatgaacag
    13081 gaatgatatc gaatggattt tgtgatgtca gatgctgctg cctgaaccaa tccagatcca
    13141 gatattcggt tattgcttcc gacgtgatgg cagtctgcga tatcgttttt actgtgctca
    13201 ggttcggaga tgatggattg ccatgtacaa ggcactatga agataacccc cattctatca
    13261 ggtcgccttc attcagacgc atacggtgat gaggttccac aatctggtcg tttaccgtac
    13321 agcaatattc ggtagactga ttgataatcc accatgcttc ttcgtggcgg gtcagagtga
    13381 gggcaatctt tacatcatca ttcaccttaa aaacaggaat ccagggagag gtaacggtga
    13441 agataatttg tgttccttca gtcgcgtaaa ttgtgttatc ggcgatcccg tgcgcctgta
    13501 ttttttggag tccccacgta atatttggtt tca
//
LOCUS       DAJTRB010000103        13284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTRB010000103 DAJTRB010000000
VERSION     DAJTRB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.103"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..246)
                     /locus_tag="OO330_003353"
     CDS             complement(<1..246)
                     /locus_tag="OO330_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5884107.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMF"
     gene            565..1146
                     /gene="mdaB"
                     /locus_tag="OO330_003354"
     CDS             565..1146
                     /gene="mdaB"
                     /locus_tag="OO330_003354"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5884108.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            1177..1491
                     /locus_tag="OO330_003355"
     CDS             1177..1491
                     /locus_tag="OO330_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5884109.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1539..3431)
                     /gene="parE"
                     /locus_tag="OO330_003356"
     CDS             complement(1539..3431)
                     /gene="parE"
                     /locus_tag="OO330_003356"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5884110.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKSVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3460..4041)
                     /gene="yqiA"
                     /locus_tag="OO330_003357"
     CDS             complement(3460..4041)
                     /gene="yqiA"
                     /locus_tag="OO330_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5884111.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4041..4868)
                     /gene="cpdA"
                     /locus_tag="OO330_003358"
     CDS             complement(4041..4868)
                     /gene="cpdA"
                     /locus_tag="OO330_003358"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5884112.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4893..5315)
                     /locus_tag="OO330_003359"
     CDS             complement(4893..5315)
                     /locus_tag="OO330_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5884113.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5316..5945)
                     /gene="nudF"
                     /locus_tag="OO330_003360"
     CDS             complement(5316..5945)
                     /gene="nudF"
                     /locus_tag="OO330_003360"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5884114.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6150..7631
                     /gene="tolC"
                     /locus_tag="OO330_003361"
     CDS             6150..7631
                     /gene="tolC"
                     /locus_tag="OO330_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5884115.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7779..8450
                     /locus_tag="OO330_003362"
     CDS             7779..8450
                     /locus_tag="OO330_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5884116.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8456..9616
                     /locus_tag="OO330_003363"
     CDS             8456..9616
                     /locus_tag="OO330_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5884117.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9654..10442)
                     /gene="ygiD"
                     /locus_tag="OO330_003364"
     CDS             complement(9654..10442)
                     /gene="ygiD"
                     /locus_tag="OO330_003364"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5884118.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10585..11358
                     /gene="zupT"
                     /locus_tag="OO330_003365"
     CDS             10585..11358
                     /gene="zupT"
                     /locus_tag="OO330_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5884119.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11416..11586)
                     /locus_tag="OO330_003366"
     CDS             complement(11416..11586)
                     /locus_tag="OO330_003366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884120.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11847..12500)
                     /gene="ribB"
                     /locus_tag="OO330_003367"
     CDS             complement(11847..12500)
                     /gene="ribB"
                     /locus_tag="OO330_003367"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5884121.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12604..12752)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12874..13164
                     /gene="ubiK"
                     /locus_tag="OO330_003368"
     CDS             12874..13164
                     /gene="ubiK"
                     /locus_tag="OO330_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5884122.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3404 a         3527 c         3204 g         3149 t
ORIGIN      
        1 gaacatttcc cgcataaaga gttttcttaa gataagaata ataagtggcg taagaagaaa
       61 aaatgctgca atcattgctt gcaatatacg atcttcgcct gaaacggaag gtatactgca
      121 aatattgcct cccgactcaa gcatccagtc atacttattg aaagcaaaaa aagaatctat
      181 taaccaataa gtaagcatta tatatagcaa tgcttcaaat attgttttaa ttttctgctt
      241 taacatatat cagtacgctc ataaaaatcc atttataatt atcaacatga cacgttgata
      301 ttttcatctt ttacttattc aaagccatat tttcaatccc caaaaaaacc gaaccaaacg
      361 tgtaaattat tccggtgcga gtaagaatta tgaggaatgg ctatcagtat tgtcattttc
      421 agaaaatatt tatcctgcat cggtgagtca gagctagatc agacttttgc taaattcgca
      481 aaagactttg cacattttgc taatttcaca gtaccgctct gtgacgtact atagtcgaca
      541 aacgtctcac cttgagatta aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa
      601 ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg
      661 cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacaa tgtcaaagcg
      721 gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg
      781 ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg tcacgggact
      841 ctgtatgcca gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg ttccggcagc
      901 ctggtacagg gcaaaaaata tatgctttct ctaacctgga acgcaccaat ggaagccttc
      961 accgaaaaag atcagttctt ccacggcgtt ggcgttgacg gtgtgtatct gccgttccat
     1021 aaagcaaacc aattcctcgg tatggaaccg ctgccgacat ttatcgctaa tgacgtgata
     1081 aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt
     1141 ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc
     1201 cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt
     1261 ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct
     1321 ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc
     1381 atcgcgcacc ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta
     1441 aaaggcgacg tgctggagat gaatatccgt attctgcagc cagggattta atcctgcctt
     1501 gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg
     1561 tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata
     1621 tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg
     1681 cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct
     1741 ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct
     1801 ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt
     1861 ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa
     1921 gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt
     1981 ttgccataac gaagctggct cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa
     2041 atatcatgca cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg
     2101 atcttacctt tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat
     2161 ccgcctgcgg agtcaccttc cacgaggaac agttcggtac ggttaaggcc ctgcgcggta
     2221 caatcagcca gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt
     2281 ttagccgcac gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc
     2341 gcctgaacgt tctggttcag ccacaggatg aaggcatctt tcaccacgcc cgaaacgaat
     2401 gccgcacact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg cggatcctgc
     2461 attttaaccg acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg
     2521 ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa caggccctga
     2581 cgcagaccat taacatgggt accgccctgc atcgttggga taaggttgac gtagctttcg
     2641 gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg
     2701 ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc
     2761 gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca
     2821 tctttaaagg taatctcaac gccggggcac agtactgatt tggccttcag cacatgcgtc
     2881 aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt catccggcca gaagtgcacg
     2941 ctggtaccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg
     3001 ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttctacgcgc
     3061 ttcgacaagg cgttaactac cgaaatcccc acgccatgca ggccgccaga gaactggtaa
     3121 tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc aaccgccggt
     3181 actccttctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat cacttctaac
     3241 gactggtcag catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg
     3301 acactgttat caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc
     3361 cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa
     3421 gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag
     3481 aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc
     3541 tatgacagtc tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc
     3601 atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat
     3661 gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt
     3721 ctcgttctga ccgagatagt ccgtcagcaa ttcaaacggg cgtaccgccg ggtttaccac
     3781 cactgcgggc agcataaaac attgcgacaa ccaggtggcg taatatcccc ccagtgacga
     3841 accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc
     3901 cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc
     3961 cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg
     4021 taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg
     4081 tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc
     4141 cagccgggcg cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag
     4201 gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata
     4261 tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc
     4321 gcgttacgca gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc
     4381 agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc
     4441 tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt
     4501 tgccattgct caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg
     4561 ctgtacatcg cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa
     4621 cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca
     4681 cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc cagcaccgcc
     4741 tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga
     4801 gtatcggtaa tttgtaaaat ccttcctctg gcctcaccag ccagaggaag ggttaacagg
     4861 ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc catcgctcca
     4921 tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg cttttcgtcg
     4981 cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg
     5041 cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg
     5101 ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc
     5161 gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg
     5221 ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc
     5281 atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct
     5341 tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc
     5401 tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt
     5461 tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt
     5521 gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt
     5581 ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca
     5641 ccctcttcaa tcatcccggc aaccatctcc agtagccagg gggtttcgct ggtgtcgtac
     5701 gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc aaagggtagc
     5761 aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg
     5821 ttgaatagac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt
     5881 tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta
     5941 agcattgtta atgtcctagc actaatagtg aattaaatgt gaatttcagc gacgtttgac
     6001 tgccgtttga gcagtcatat gttaaattga ggcacattaa cgccctatgg cacgtaacgc
     6061 caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc
     6121 taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc
     6181 tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc
     6241 aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg
     6301 aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca
     6361 cctatagcaa cggctaccgc gacgcgaacg gcatcaactc gaacgcgacc agtgcgtccc
     6421 tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa
     6481 aagcagcagg gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca
     6541 ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac
     6601 aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg
     6661 tagcgatcac cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag
     6721 tgaccgcacg taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact
     6781 actatccgga actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg
     6841 ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga
     6901 gccaggacct ggagcgcgag caaattcgcc aggcgcagga tggtcactta ccgactctgg
     6961 atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg
     7021 ccgctggtac ccagtatgac gatagcaata tgggccagaa caaagttggc ctgagcttct
     7081 cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggca cagtacaact
     7141 ttgttggtgc cagtgagcaa ctggaaagcg cacatcgtag cgtcgtgcaa accgtacgtt
     7201 cctccttcaa caacattaat gcttctatca gtagtattaa cgcctacaaa caagccgtag
     7261 tttccgcgca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca
     7321 ttgttgatgt gttggatgcg accaccacgc tgtacaacgc caagcaagag ctggcgaatg
     7381 cgcgttataa ctacctgatt aatcagctga atattaagtc agccctgggt acgttgaacg
     7441 agcaggattt gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa
     7501 acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc
     7561 cggcacccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt
     7621 tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga
     7681 tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta
     7741 ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac
     7801 gccacgcatc gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg
     7861 ttgccactgt ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc
     7921 aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt
     7981 acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact
     8041 gtgttgctga atttggtgaa ggtcagtgtc agcaagcacc agcccaggct ggcatggcac
     8101 cagaaaacca ggcgcaggct cagcaatcca gcgggagttt ctggatgccg ctgatggccg
     8161 gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct
     8221 cctcgaaaaa cccggccagt ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact
     8281 atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacggcaatg gcaccaaaac
     8341 cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca
     8401 ctatgcagcg tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga
     8461 aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat acggtttcaa
     8521 ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct
     8581 cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt
     8641 ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcatac caaaacacac
     8701 ctggagtttt gtgcgtcaat catggctgac tcaccagcca tcgctttatt cgcgtcttga
     8761 tctggcgtgg gatggcactg gcgaacctaa acttctggaa aataacgccg atacgccaac
     8821 gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg
     8881 taacttgtcg gagggcagcg accagtttaa cagtctgcaa gaaaaactga tcgatcgctt
     8941 cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac
     9001 ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcaaccgaag ctgaaatcgc
     9061 caccgagttc ctctacatcg acgagatagg tttaggcgaa aaaggccagt tcacggattt
     9121 acaggatcag gtgatttcta acctgttcaa gctttatccg tgggaattta tgctgcgcga
     9181 aatgttctcc accaagctgg aagatgctgg cgtacgctgg ctggaaccgg cgtggaagag
     9241 cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga atcacccgaa
     9301 cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa
     9361 accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga
     9421 agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc
     9481 gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg
     9541 aattggcatt cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca
     9601 tatttttgtt gaataagcca cgataccgga tggcactcgc catccggtaa ttgttagcct
     9661 atctgcaccg acagcatact taaactgccc atttctatac catcgaccgg aatggtaatt
     9721 ggctcctgcc catcccacac acctaacaca tacaacaacg gcagatagtg ctctggcgtt
     9781 gggttcgata acgcgccacc ttcatggtcg agataattca ccagaggatg ctgctctacc
     9841 ggcccttgcc acgtcagatt cgctttcaca tactcattaa acgacgtcgc ccacggatac
     9901 ggtgaactat caccgtgcca cttcactgtg cgcaggttat gcaccacgtt accgctggcg
     9961 accaacatta ttccttcatc acgcagcgcc gcgagtttgc gccccatttc gaaatgccag
    10021 gcggcaggtt tgctactgtc gatactcaac tgcaccatag ggatatcggc gtcaggatac
    10081 atcttaatca gcacgcccca cgagccgtgg tcaaagcccc aggcttcttt atccagcgtc
    10141 accgggatcg gcgctaacag ctcaaccaga cgctgtgcca gcgcaggcga acccggagca
    10201 ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc
    10261 gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc
    10321 gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct
    10381 tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac
    10441 atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg
    10501 gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag
    10561 gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg
    10621 gcagccacgt ttattggcgc gtttctcggc gttctcggac aaaaaccctc gaaccgctta
    10681 ctggcgtttt cgctaggttt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg
    10741 cttcctgccg cactagcagc tgaaggaatg tcgccggtgt tgggttatgg aatgtttatc
    10801 ttcggtctgc ttggctactt tggcctggac cgcatgttgc cgcatgcgca tccgcaggat
    10861 ttaatgcaaa aatcggtgca gctgttgcca aaatcgatca agcgcacagc cattctgctc
    10921 actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc
    10981 agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa
    11041 ggtctggcag tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg
    11101 tgggcgggga tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc
    11161 ggtagcatga tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg
    11221 gtggcgctct cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac
    11281 cccagctatg gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa
    11341 acggcgggaa ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc
    11401 agcttcacga aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta
    11461 tttgttccgc gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt
    11521 caaatgcagg aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat
    11581 aaacatcctt ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat
    11641 atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat
    11701 cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt
    11761 aacatcttgc atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga
    11821 ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat
    11881 gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac
    11941 tctggtgcac gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt
    12001 ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt
    12061 acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt
    12121 tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg
    12181 gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg
    12241 ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg
    12301 caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct
    12361 gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg
    12421 cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag
    12481 gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag
    12541 ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag
    12601 aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc
    12661 tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg
    12721 gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg
    12781 gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg
    12841 gtactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa
    12901 atcgctcgcc aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg
    12961 gagaaaaaaa tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt
    13021 gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgctgctt
    13081 gaacaaagaa tcagcgaact ggaaaatcgc agtactgaga tcaaaaagca gccagatcca
    13141 gagactctcc ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa
    13201 tcctctaatt tacagtgttt tctattacat tatcgcttaa tcccattcag agtatcatca
    13261 caaaaatgaa tgattgcaat tgaa
//
LOCUS       DAJTRB010000104        13234 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTRB010000104 DAJTRB010000000
VERSION     DAJTRB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.104"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..983)
                     /gene="kdgT"
                     /locus_tag="OO330_003369"
     CDS             complement(<1..983)
                     /gene="kdgT"
                     /locus_tag="OO330_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5884123.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTLILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1243..1863)
                     /gene="sodA"
                     /locus_tag="OO330_003370"
     CDS             complement(1243..1863)
                     /gene="sodA"
                     /locus_tag="OO330_003370"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5884124.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2148..3182
                     /gene="rhaT"
                     /locus_tag="OO330_003371"
                     /pseudo
     CDS             2148..3182
                     /gene="rhaT"
                     /locus_tag="OO330_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3179..4045)
                     /gene="rhaR"
                     /locus_tag="OO330_003372"
     CDS             complement(3179..4045)
                     /gene="rhaR"
                     /locus_tag="OO330_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5884125.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4101..4937)
                     /gene="rhaS"
                     /locus_tag="OO330_003373"
     CDS             complement(4101..4937)
                     /gene="rhaS"
                     /locus_tag="OO330_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5884126.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5225..6694
                     /gene="rhaB"
                     /locus_tag="OO330_003374"
     CDS             5225..6694
                     /gene="rhaB"
                     /locus_tag="OO330_003374"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5884127.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADVCGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6691..7950
                     /gene="rhaA"
                     /locus_tag="OO330_003375"
     CDS             6691..7950
                     /gene="rhaA"
                     /locus_tag="OO330_003375"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5884128.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8192..9016
                     /gene="rhaD"
                     /locus_tag="OO330_003376"
                     /pseudo
     CDS             8192..9016
                     /gene="rhaD"
                     /locus_tag="OO330_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9026..9340
                     /gene="rhaM"
                     /locus_tag="OO330_003377"
     CDS             9026..9340
                     /gene="rhaM"
                     /locus_tag="OO330_003377"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5884129.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9641..10087
                     /locus_tag="OO330_003378"
     CDS             9641..10087
                     /locus_tag="OO330_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5884130.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10098..11549
                     /locus_tag="OO330_003379"
     CDS             10098..11549
                     /locus_tag="OO330_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5884131.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDISKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11539..12609
                     /locus_tag="OO330_003380"
     CDS             11539..12609
                     /locus_tag="OO330_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5884132.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPSVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12609..>13234
                     /locus_tag="OO330_003381"
     CDS             12609..>13234
                     /locus_tag="OO330_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5884133.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3156 a         3455 c         3482 g         3141 t
ORIGIN      
        1 tatttcaccg tacctaaaat ttcgattttc gctgctctgg ctttgacttt acgtgaccag
       61 atagaggtaa gaattggcac cagaatcaaa gtcacaatga ccgccgtcgc taccagcgaa
      121 gttgctgccg gagccatcgg tttaaacgca ggcaccattt ctgcaatcag cacaggtgtc
      181 gctaccgcgg cccctgcgga actggaagcg gcaattccgg cagtgccatc gccaccgcca
      241 atcaatttat cggcgataat caacggaata ccggtcacga taattactgc cacacccaac
      301 aggatcccca gcaaaccagt ctgggcaatc acagtcaaat caatggtatt gcccagcgcg
      361 aaggcaaaga atggaatcag cgtttgcacc gctttgctga aaaattctcg taattcaggg
      421 tcaagattcc ccagggcaaa gcccaccagg aacggtaata ctgcgccgac gaaaacatgc
      481 ggttcaaacg aggcaatccc ggcagtgccc agaataatca tcgtcatgag cggcccggac
      541 tccaacgaca tcaacacaaa tgccccagct tcttctttcg taccgtactg ctgcatgatg
      601 gaagcgtaaa gtccgccgtt ggtcatatcc atcgccgcca ccagcgccag cgttgaaagt
      661 ccggcaaaga atccaacttc aacaccatgt tccggaataa tgcgcgaggc aattgccgca
      721 accacccacg cgacggcaat tttagttacc accagcgtac cggatttacg cagtaccgtt
      781 cccgtcgcgc ttaattttat tgacgccccc atgcaaaaaa accacaccgc cagaatgggc
      841 accgtaccgg taatcatccc gttggtgaat gatccaaaat atttccccgc ccccggcgag
      901 aaggtgtggc acagtgcgcc aaggaatagc gggacgagca tcatcccccc cgggattttc
      961 tcaatcgagc gttttatctg catttccatc acctgtcggc atatttttaa gggctgatgc
     1021 gtaattcctc aatggagcat cagtgttgat ggaaacgaag atagacattc atttaatcaa
     1081 taaaagtgat caaaatcaca tcaattgaaa cgctgtttta tttttataag accattgttt
     1141 ttattttgaa attgatcaca aaaaaaccac cgccgttggc gatggttcat tttttaagct
     1201 gatatgcggc ctatacgcct cattgcagca ggcggcaaat gattattttt tcgccgcaaa
     1261 acgtgccgct gcttcgtccc agttcaccac gttccagaac tctttgatgt agtccggacg
     1321 gcggttctgg aatttcaggt agtaagcatg ttcccacaca tccaggccca gaatcgggaa
     1381 gccggaagcg ccagaaatag cttcacccat cagcggagaa tcctggttag cagtagaaac
     1441 caccgccagt ttatcgcctt tcagcaccag ccatgcccag ccggaaccaa agcgggaagc
     1501 tgccgctttt tcaaattctg ctttgaagtt atcaacggag ccgaagtcac gttcgatagc
     1561 cgctttcagg tcaccctgca gggtggtgcc ttttttcaga cctttccaga acaggctgtg
     1621 gttagcgtga ccgccagcgt tgttgcgcag tacggttttc ttgtctgctg gcagctggtc
     1681 cagtttggtg atcagctctt caaccggcag gttggcaaat tctggcaggc tttccagcgc
     1741 cgcgttggcg ttgtttacgt aggtctgatg gtgtttggtg tggtggattt ccatggtctg
     1801 cttatcgaag tgcggttcca gggcatcgta agcatacggc agggatggca gggtatagct
     1861 catattcatc tccagtattg tcgggcggcc gattgttaat gccgcgtaag cagttggttc
     1921 attatagtta attaaatgat attgaaaatg attatcaatg ccgtactttt cgtaagggta
     1981 tggttttgca ggaaaatgcc cgagatgtga agcaaatcac ccacttaatg ccgtgattgc
     2041 cagtaaatcg acaacggcgg caacaggcga aaggttaatc gacagcacga tttttacact
     2101 catctcgtcg gagatgtgaa gcgacgaaaa atgatgagga taagaagatg agtaacgcga
     2161 ttacgatggg gatattttgg catttgatcg gcgcggccag tgcagcctgt ttttacgctc
     2221 cgttcaaaaa agtaaaaaaa tggtcatggg aaaccatgtg gtcagtcggt gggattgttt
     2281 cgtggattat tctgccgtgg gccatcagcg ccctgttact accggatttc tgggcgtatt
     2341 acagctcgtt tagtctctct acgctgctgc ctgtttttct gttcggcgct atgtggggga
     2401 tcggtaatat caactatggc ctgaccatgc gttatctcgg catgtcgatg ggaattggca
     2461 tcgccattgg cattacgttg attgtcggta cgctgatgac gccaattatc aacggcaatt
     2521 tcgatgtgct gattaacacc gaaggcggac gcatgacgtt actcggcgtt ctggtggcgc
     2581 tgattggcgt agggattgta actcgagccg ggcagttgaa agagcgcaag atgggcatta
     2641 aagccgaaga gttcaatctg aaaaaagggc tggtgctggc ggtgatgtgc ggcattttct
     2701 ctgccgggat gtcctttgcg atgaacgccg caaaaccgat gcatgaagcc gctgccgcac
     2761 ttggcgtcga tccactgtat gttgctctgc caagctatgt tgtcatcatg ggcggcggcg
     2821 cgatcattaa cctcggtttc tgttttattc gtctggcaaa agtgaaggat ttgtcgctaa
     2881 aagccgactt ctcgctggca aaaccgctga tcattcacaa tgtgttactc tcggcactgg
     2941 gcgggttgat gtggtatctg caattctttt tctatgccta gggccacgcc cgcattccgg
     3001 cgcagtatga ctacatcagt tggatgctgc atatgagttt ctatgtattg tgcggcggta
     3061 tcgtcgggct ggtgctgaaa gagtggaaca atacaggacg ccgtccggta acggtgttga
     3121 gcctcggttg tgtggtgatt attgtcgccg ctaacatcgt cggcatcggc atggtgaatt
     3181 aatctttctg cgaattgaga tgacgccact ggctgggcgt catcccggtt tcccgggtaa
     3241 acaccaccga aaaatagtta ctatcttcaa agccacattc ggtcgaaata tcactgatta
     3301 acaggcggct atgctggaga agatattgcg catgacacac tctgacctgc cgcagatatt
     3361 gattgatggt cattccagtc tgctggcgaa attgctgacg caaaacgcgc tcactgcacg
     3421 atgcctcatc acaaaattta tccagcgcaa agggactttt caggctagcc gccagccggg
     3481 taatcagctt atccagcaac gtttcgctgg atgttggcgg caacgaatca ctggtgtaac
     3541 gatggcgatt cagcaacatc accaactgcc cgaacagcaa ctcagccatt tcgttagcaa
     3601 acggcacatg ctgactactt tcatgctcaa gctgaccgat aacctgccgc gcctgcgcca
     3661 tccccacgct acctaagcgc cagtgtggtt gccctgcgct ggcgctaaat cccggaatcg
     3721 ctccctgcca gtcaagattc agcttcagcc gctccgggca ataaataata ttctgcaaaa
     3781 ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc atgaatataa aagagatcgc
     3841 cacgggtaat gcgataagga cgatcgttga gtacatgcag accattaccg cgccagacaa
     3901 tcaccagctc acaaaaatca tgtgtatgtt cggcaaagac atcttgcgga taacggtcag
     3961 ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt tttaactgat
     4021 gcgccaccgt ggctacctcg gccagagaac gaagttgatt attcgcaata tggcgtacaa
     4081 atacgttgag aagattcgcg ttattgcaga aagccatccc gtccctggcg aatatcacgc
     4141 ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatcca
     4201 cagcgatagg cgatgtcagt gacgctggcc tcgctgtggc gtagtagatg tcgggctttc
     4261 atcagtcgca ggcggttcag gtatcgctga ggcgtcagtc ccgtttgctg cttaagctgc
     4321 cgatgtagcg tacgcagtga aagagaaaat tgatccgcca cggcatccca attcacctca
     4381 tcggcaaaat ggtcctccag ccaggccaga agcaagttga gacgtgatgc gctgttttcc
     4441 aggttctcct gcaaactgct tttacgcagc aagagcagta attgcataaa caagatctcg
     4501 cgactggcgg tcgagggtaa atcattttcc ccttcctgct gttccatctg tgcaaccagc
     4561 tgtcgcacct gctgcaatac gctgtggtta acgcgccagt gagacggata ctgcccatcc
     4621 agctcttgtg gcagcaactg attcagcccg gcgagaaact gaaatcgatc cggcgagcga
     4681 tacagcacat tggtcagaca cagattatcg gtatgttcat acagatgccg atcatgatcg
     4741 cgtacgaaac agaccgtgcc accggtgatg gtatagggct gcccattaaa cacatgaata
     4801 cccgtgccat gttcgacaat cacaatttca tgaaaatcat gatgatgttc aggaaaatcc
     4861 gcctgcggga gccggggttc tatcgccacg gacgcgttac aagacggaaa aaaatccaca
     4921 ctatgtaata cggtcatact ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg
     4981 aggtcaggtt cttaccttaa attttcgacg gaaaactacg taaaaaacgt cgatttttca
     5041 agatacagcg tgaattttca ggaaatgcgg tgagcatcac atcaccacaa ttcagcaaat
     5101 tgtgaacatc atcacgttca tctttccctg gttgccaatg gcccattttc ctgtcagtaa
     5161 cgagaaggtc gcgaattcag gcgcttttta gactggtcgt aatgaaattc agcaggatca
     5221 cattatgacc tttcgcaatt gtgtcgccgt cgatctcggc gcatccagtg ggcgcgtgat
     5281 gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc atcgttttaa
     5341 caatgggctg catagccaaa acggttatgt cacctgggat gtggatagcc tggaaagtgc
     5401 cattcgcctt ggattaaaca aggtgtgcga ggaagggatt cgtatcgata gcattgggat
     5461 tgatacctgg ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg tgggcctgcc
     5521 cgttgcttat cgcgatagcc gcaccaatgg cctgatgccg caggcacaac agcaactcgg
     5581 caaacgcgat atttatcagc gtagcggcat ccagtttcta cccttcaata cgctttatca
     5641 gttgcgtgcg ctgacggagc aacaacctga acttattcca cacattgctc acgctctgct
     5701 gatgccggat tacttcagtt atcgcctgac cggcaagatg aactgggaat ataccaatgc
     5761 caccaccaca caactggtca atatcaatag cgacgactgg gacgagtcgc tactggcgtg
     5821 gagcggggcc aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg tcattgggca
     5881 ctggatttgc ccgcagggta atgagattcc ggtggtcgcc gttgccagcc atgataccgc
     5941 cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc gcttatctct cttctggcac
     6001 ctggtcattg atgggcttcg aaagccagac gccatttacc aatgacacgg cgctggcagc
     6061 caacatcacc aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa atattatggg
     6121 cttatggctg cttcagcgag tgcttcagga acggcaaatc aacgatctcc cggcgcttat
     6181 cgccgcgaca caggcacttc cggcctgccg cttcatcatc aatcccaatg acgatcgctt
     6241 tattaatcct gacgagatgt gcagcgaaat tcaggctgcg tgtcgggaaa cggcgcaacc
     6301 gatcccggga agtgatgctg aactggcgcg ctgtattttc gacagtctgg cgctgctgta
     6361 tgccgatgtg ttgcatgagc tggcgcagct gcgcggtgaa gatttctcgc aactgcatat
     6421 tgtcggcggc ggctgccaga acacgctgct caaccagcta tgcgccgatg tctgcggtat
     6481 tcgggtgatc gccgggcctg ttgaagcctc aacgctcggc aatatcggca tcgagttaat
     6541 gacgctggat gaactcaaca atgtggatga tttccgtcag gtcgtcagca ccaccgcgaa
     6601 tctgaccacc tttaccccta atcctgacag tgaaattgcc cactatgtgg cgcagattca
     6661 ctctacacga cagacaaagg agctttgcgc atgaccactc aactggaaca ggcctgggag
     6721 ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct gcgccaactt
     6781 gatcgtttac ccgtttcaat gcactgctgg cagggcgatg atgtttccgg ttttgaaaac
     6841 ccggaaggtt cgctgaccgg ggggattcag gccacaggca attatccggg caaagcgcgt
     6901 aatgccagtg agctacgtgc cgatctggaa caggctatgc ggctgattcc ggggccgaaa
     6961 cggcttaatt tacatgccat ctatctggaa tcagatacgc cagtctcgcg cgaccagatc
     7021 aaaccagagc acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct cggtctggat
     7081 tttaacccct cctgcttttc gcatccgcta agcgccgatg gctttacgct ctcgcatccc
     7141 gacgacagca ttcgccagtt ctggattgat cactgcaaag ccagccgtcg cgtttcggcc
     7201 tattttggcg agcaactcgg cacaccatcg gtgatgaaca tctggatccc ggatggtatg
     7261 aaagatatca ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc actggatgag
     7321 gtgatcagcg agaagctaaa ccctgcgcac catatcgacg ccgttgagag caaattgttt
     7381 ggcattggcg cagagagcta cacggttggc tccaatgagt tttacatggg gtatgccacc
     7441 agccgccaga ctgcgctgtg cctggacgcc gggcacttcc acccgactga agtgatttcc
     7501 gacaagattt ccgccgccat gctgtatgta ccgcagttgc tgctgcacgt cagccgtccg
     7561 gttcgctggg acagcgatca cgtagtgctg ctggatgatg aaacccaggc aattgccagt
     7621 gagattgtgc gtcacgatct gtttgaccgg gtgcatatcg gccttgactt cttcgatgcc
     7681 tctatcaacc gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa agccctgctg
     7741 cgtgcgttgc tggaacctac cgctgagctg cgcaagctgg aagcggcggg cgattacact
     7801 gcgcgtctgg cactgctgga agagcagaaa tcgttgccgc ggcaggcggt ctgggaaatg
     7861 tattgccaac gtcacgatac gccagcaggt agcgaatggc tggagagcgt gcgggcttat
     7921 gagaaagcga ttttgagcca gcgtgggtaa acactgccgg atgcggcacg agcgccttat
     7981 ccggcctacg ggtcgaatgt ccatgcgggc tgtcaccctc accccggccc tctccctgaa
     8041 agggcgaggg agaaaaacat agcctcgtgc ggcaacggag ctgaacaccg ctccggacag
     8101 tcccctcgcc cctacgggga gagggttagg gtgaggggaa caagccgcac catgtctaaa
     8161 caaggcaacc taatttacag gaacacagaa catgcaaaac attacccagt cctggtttgt
     8221 ccagggaatg atcaaagcca ccaccgatgc ctagctgaaa ggctgggacg agcgcaacgg
     8281 cggcaacctg acgctgcgcc tggatgacgc cgatatcgcg ctttatcacg acaacttcca
     8341 cccacaaccg cgctatattc cgctcagcca acccatgcct ttactggcaa atacgccgtt
     8401 tattgtcacc ggctccggca aattcttccg taacgtccag ctcgatcctg cagctaactt
     8461 aggcgtcgta aaagtcgaca gcgatggcgc gggctaccat attctctggg ggttaaccaa
     8521 cgaagccgtt ccaacttctg aacttccggc tcactttctc tcccactgcg agcgcattaa
     8581 agccaccaac ggtaaagatc gggtgatcat gcactgccac gccaccaacc tgatcgccct
     8641 cacctatgta ctggaaaacg acaccgcggt ctttactcgc caactgtggg aaggcagcac
     8701 cgaatgtctg gtagtattcc cggatggcgt tggcattctg ccgtggatgg tgcccggcac
     8761 ggacgaaatc ggccaggcga ccgcacaaga gatgcaaaaa cattcgctgg tgttgtggcc
     8821 cttccacggt gtgttcggtt ccggaccgac gctggatgaa gcctttggtt taatcgacac
     8881 cgcagaaaaa tcagcacaag tattagtgaa ggtttattcg atgggcggca tgaaacagac
     8941 catcagccgt gaagagttga tagcgctcgg caagcgcttc ggcgttacac cactagccag
     9001 tgcgctggcg ctgtaaggag caaacatgat ccgcaaagcc tttgtcatgc aggtaaaccc
     9061 cgacgcccac gaagagtatc agcgtcggca taatcccatc tggccggaac tggaagcagt
     9121 gttgaaatct cacggtgtgc ataactacgc catttacctc gacaaagcgc gtaatctgct
     9181 gtttgccatg gtagagattg aatctgaaga acggtggaat gcggtagcca gtactgatgt
     9241 ttgccaacgt tggtggaaat atatgaccga tgttatgccc gctaacccag ataacagccc
     9301 ggtgagtagc gagctgcaag aagtgtttta cctgccgtaa ttgcccaatg tgccggagat
     9361 gcctccggca caatatttta aatttcgatt tgaaaaatta aacatattac atctcccggt
     9421 aagttatatt tccctgatac attgtgagta aatcacaaaa ataatgaata acccattaat
     9481 gattcatgtg gtttatttaa ataaccatta tgtgcattac tccgcaaatc tgacctttca
     9541 ctctgtctca aattgtcatt gtttaccgac atatcgcctc tcatcactta atcgctccac
     9601 cattacaatt gattaaaata tattttatgg agtccgatta atggcagctc ttactgcaag
     9661 ctgtattgac ctgaatattc agggcaatgg cgcttattcc gttctgaagc agttggcgac
     9721 aatagcgtta caaaacggtt ttatcaccga ctcacaccag ttcctgcaaa ccctgctgct
     9781 gcgcgaaaaa atgcactcta ctggatttgg ttccggtgtc gcagtgccgc acggtaaaag
     9841 cgcctgcgtt aaacaaccgt tcgtattatt cgcccgcaaa gcgcaggcta ttgactggaa
     9901 agccagcgat ggcgaagacg tcaattgctg gatctgcctc ggcgtgccgc aaagcggcga
     9961 agaggatcag gtcaaaatca tcggcacact gtgtcgcaaa attattcacc aggaatttat
    10021 tcatcaactg caacagggcg ataccgacca ggtgctggcc ttgttaaatc aaaccctcag
    10081 ctcataagga agtggcgatg gagtcatcct tacgtattgt cgcgatcacc aactgccccg
    10141 ccgggatcgc tcacacctac atggtggcgg aagccctgga acagaaagcc cgttctctcg
    10201 gccataccat aaaagtggaa actcaagggt ccagtggcgt tgaaaaccgc ttatccagcg
    10261 aagagattgc cgctgccgat tacgtcattc ttgctaccgg gcgtggcctg agcggtgatg
    10321 atcgcgcgcg gtttgccggg aagaaagttt atgagattgc catctccctg gcgttgaaaa
    10381 atatcgacca gattttcagc gaattaccga caaactcgca gctttttgcc gcagatagcg
    10441 gcgtgaagct tggcaaacag gaagtgcaaa gcggcagcgt gatgagccac ctgatggcgg
    10501 gcgtctctgc ggcgctgccg tttgtcatcg gcggtggtat cctggtggcg cttgccaaca
    10561 tgctggtgca gttcggtttg ccgtataccg atatttcgaa aggcgcgccg tcgtttacct
    10621 gggtagttga atccatcggt taccttggct tcacctttat gatccccatc atgggcgctt
    10681 atattgcctc gtcgattgcc gataaaccgg ctttcgctcc ggcgtttctg gtttgctatc
    10741 tggcgaacga caaagcgctg ctcggcaccc agtcgggtgc gggattcctc ggcgcggtgg
    10801 tgctggggct ggcgattggc tatttcgtct tctggttccg caaagtgcgc cttggcaaag
    10861 cattacaacc gctgctcggt tcaatgctga tcccgttcgt tactctgctg gttttcggcg
    10921 tgctcactta ctacgttatc gggccggtga tgtccgacct catgggcggg ctgctccact
    10981 tcctgaatac cattcctcca tcaatgaagt ttgcggcagc gtttctggtc ggtgcgatgc
    11041 tggcgttcga tatgggcggc cccatcaata aaaccgcctg gttcttctgc ttctcactgc
    11101 tggaaaaaca catttatgac tggtacgcca tcgtcggcgt tgtcgcgttg atgccgcccg
    11161 ttgcggcggg tcttgcgacg ttcattgcgc ccaaactgtt tacccgacag gaaaaagagg
    11221 ctgccagtag tgccattgtg gtcggtgcca cagtcgcaac cgaaccggct ataccttacg
    11281 ctctggcagc tccgttgccg atgattaccg ccaatacgct ggcgggcggc attaccggcg
    11341 tgctggtgat cgctttcgga atcaaacgtc tggcacctgg tttgggtatc tttgacccgc
    11401 tgatcggcct gatgtcgccg gtaggttcgt tttatctggt gctggcgatt ggcctcgcgc
    11461 tgaacatttc attcattatc gtcctgaaag gattgtggtt acgccgcaaa gcgaaagccg
    11521 cgcaacagga gcttgtccat gaacattgag ttactgcaac agttgtgcga agccagcgcc
    11581 gttagcggcg atgaacagga agttcgcgac atcctgataa acacgctgga accttgcgtt
    11641 aatgaaatca cctttgatgg tctgggcagc tttgttgccc gtaagggaaa taaaggtcca
    11701 aaagttgccg ttgtcgggca tatggatgaa gtcggcttta tggtcaccca catcgacgag
    11761 agcggttttc tgcgtttcac caccattggt ggctggtgga atcagtcgat gctcaaccat
    11821 cgggtaacca tacgcacaca caagggagtg aaaatcccta gtgtgattgg tttcgtcgcg
    11881 cctcatgcgt taacggaaaa gcaaaggcaa cagccgctgt catttgatga gatgttcatt
    11941 gatattggcg caaacagtcg cgatgaggtg gaaaaacgcg gtgtggcgat gggcgatttt
    12001 attagcccgg aagccaattt tgcctgctgg ggcgaagata aagtggtcgg caaggcgctg
    12061 gataaccgca tcggctgcgc aatgatggct gaactattgc agacggtgaa taatcccgaa
    12121 attacgctgt atggcgtcgg cagcgtggaa gaagaagttg ggctacgcgg ggcacaaacc
    12181 tcggcggaac acattaaacc ggatgtagtg atcgtgctgg ataccgctgt cgcaggcgat
    12241 gttccgggca ttgataacat taaatacccg ctgaaactgg gccaggggcc ggggctgatg
    12301 ctgtttgaca agcgctactt ccccaaccag aaactggtag cagcgttaaa aaactgtgcc
    12361 gcacataacg atttaccgct gcaattttcc accatgaaaa ccggagcgac ggatggcggg
    12421 cgctacaacg tgatgggcgg cgggcgtccg gttgttgcgc tgtgcctgcc aacccgctat
    12481 ctgcacgcta acagcggcat gatttcaaaa gccgattacg atgctctgct cacgttgata
    12541 cggggttttc tgacgacctt aaccgcggag aaagtcaacg cgtttagcca gttccgtcag
    12601 gtggattaat gctaaacgaa cgccagttaa agattgtcga tctgctggag caacagccgc
    12661 gcacgcctgg tgagctggcg caacagactg gcgtttcagg caggaccatc ctgcgtgata
    12721 ttgactatct caactttacc cttaacggca aagcccgcat tttcgccagt ggcagtgcgg
    12781 gctatcagct ggaaatcttc gagcgccgca gcttttttca gttgctgcaa aagcacgata
    12841 acgacgatcg gctgctggcg ctgttattac tgaatacttt cactccccgt gcgcaactcg
    12901 cctcggcgct taatttgcca gaaacgtggg tagcagagcg tctgccccag ttaaaacagc
    12961 gttatgaacg cacttgttgc ctggccagcc gccctggttt gggccatttc attgatgaga
    13021 cagaagagaa actcgttatc ttgctggcga acttgctgcg caaagatccg tttttaattc
    13081 cgctggcggg cataacacga gacaaccttc agcatttatc cacggcctgc gacaaccaac
    13141 accgctggcc gctcatacag ggtgattatc tctccagcct gattctggcg atttacgccc
    13201 tgcgtaatca actgaccgat gagtggccgc aata
//
LOCUS       DAJTRB010000105        12924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTRB010000105 DAJTRB010000000
VERSION     DAJTRB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.105"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(145..1596)
                     /gene="uxaB"
                     /locus_tag="OO330_003382"
     CDS             complement(145..1596)
                     /gene="uxaB"
                     /locus_tag="OO330_003382"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5884134.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1823..3241)
                     /locus_tag="OO330_003383"
     CDS             complement(1823..3241)
                     /locus_tag="OO330_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5884135.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3395..3739)
                     /locus_tag="OO330_003384"
     CDS             complement(3395..3739)
                     /locus_tag="OO330_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5884136.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3739..4665)
                     /gene="glsB"
                     /locus_tag="OO330_003385"
     CDS             complement(3739..4665)
                     /gene="glsB"
                     /locus_tag="OO330_003385"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5884137.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4729..5664)
                     /locus_tag="OO330_003386"
     CDS             complement(4729..5664)
                     /locus_tag="OO330_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5884138.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5744..6859
                     /gene="yneK"
                     /locus_tag="OO330_003387"
     CDS             5744..6859
                     /gene="yneK"
                     /locus_tag="OO330_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5884139.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRPLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            7009..8199
                     /gene="ydeA"
                     /locus_tag="OO330_003388"
     CDS             7009..8199
                     /gene="ydeA"
                     /locus_tag="OO330_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5884140.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8224..8889)
                     /gene="marC"
                     /locus_tag="OO330_003389"
     CDS             complement(8224..8889)
                     /gene="marC"
                     /locus_tag="OO330_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5884141.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9101..9535
                     /gene="marR"
                     /locus_tag="OO330_003390"
     CDS             9101..9535
                     /gene="marR"
                     /locus_tag="OO330_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5884142.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9555..9938
                     /gene="marA"
                     /locus_tag="OO330_003391"
     CDS             9555..9938
                     /gene="marA"
                     /locus_tag="OO330_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5884143.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9970..10188
                     /gene="marB"
                     /locus_tag="OO330_003392"
     CDS             9970..10188
                     /gene="marB"
                     /locus_tag="OO330_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5884144.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10219..11118)
                     /gene="eamA"
                     /locus_tag="OO330_003393"
     CDS             complement(10219..11118)
                     /gene="eamA"
                     /locus_tag="OO330_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5884145.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11382..12500
                     /gene="ydeE"
                     /locus_tag="OO330_003394"
     CDS             11382..12500
                     /gene="ydeE"
                     /locus_tag="OO330_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5884146.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12627..12722
                     /gene="mgtS"
                     /locus_tag="OO330_003395"
     CDS             12627..12722
                     /gene="mgtS"
                     /locus_tag="OO330_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5884147.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3404 a         3302 c         2951 g         3267 t
ORIGIN      
        1 ttttcaattt cctatatgtc aagcttacaa cattaacagg cgctgcatgt gacacctgtt
       61 aatgataaag ggatatagga tgttgtaact aacatagtga aataagaaac ccggtcgaaa
      121 ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc gcatcccttt
      181 ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca cttgcgtcag
      241 gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct gggtaccgat
      301 aacacggtca cgatgctggc tccagagttg ctgataacgt tccagccagt gtgcatcgtc
      361 ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca atgccgctaa
      421 tgcgaaagta aggcgcgccg gaagtgtgcc atttgccttc tgccctgcca gcagctgcgg
      481 caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca gctgatgctt
      541 aatgtacggg ttacggaaac gcccggtgac tgcactggcg aaagattcca gttcatcacg
      601 aggcaaatcc agtaccggaa taatttcttc gtagatggct ttttcaacga atgcgcaaat
      661 ttcagcatcg ttcatcgctt cacctacggt atccagccct gcctggaacg ccaccggcac
      721 cagcgcggtg tgcgcaccgt tgaggattgc cactttgcgt tctttatacg gtttaatatc
      781 gtcaacaatc agcacgttga gcggatattt gtccaggcgc agctcagtcg ctaaggattt
      841 cggcccctga atcacaaaca ggtaaaagtg ttcagcggtg tcgaggaaac catcgtgata
      901 acccagttct tcttccagtt tagccacttc atcgcgcgga taaccggtaa cgatacggtc
      961 caccagcgta gaacagaagc tgttagcctg atccagccat tgaataaatg cttctggcag
     1021 tgcccactct tgcgcatagc gcagcaccag ttcacgcaac gcgtcgccat tgtagtcaat
     1081 caactcacac ggaatgatga tccaaccttt atccagcgca ccgttgaaat ggctaaagcg
     1141 ttcgaacagc agacgggtca gttttgccgg atagcttacc gctggcgcgt catcgaattt
     1201 atcgcccgca tggtagctga tacccgcttc ggtggtgtta gagaaaacaa agcgcatttc
     1261 cgggttgtgc gccagtttca ggaattcatc gtattcactg tagacgctga tttcacgatt
     1321 aaccgagcga atcagacgcg cgtcgctgac cgcttccccc ttctcattca ggccacggat
     1381 aatggtggtg tacagaccat cctgcgtgct cagtgacggc gggaatgaag tttcaatcgg
     1441 acgaacaacg accacgccag aattcagatc ggtgtgctca ttcaggagat cgatttgcca
     1501 gtcaacaaag gcgcgcagga agttaccttc accaaactga atgatacgtt ctggatactg
     1561 tgcaccggga aaatcgcgac gatttagtgt tttcacaatg ggttcccttc tgattagtca
     1621 cacaacctgt ttgaattggt gcgacaggtt agcaaacttt aatacgccga acccctgttt
     1681 tgatcaactc ctgatgatta atgagcagtt ttatgagaaa agtgtggcgc ggatcatggt
     1741 ttaatcgagg aaatacgcct tttcctggat cataaagtgg tagaacacat tgcattcaaa
     1801 tcgcgcgaaa tgaataaaga tgtcagacaa cttcctcacc gtaacgcata gtgctggtac
     1861 ggttgcgccc atctttcttc gagcgataca gacacgtatc ggcttcgacc atcaatttat
     1921 taaaatcatc ggtcagcgtt cggtacgatg cgcgaccact accgacgcca atacttaccg
     1981 tgagataaag cgttttttgt tgccaggtga atggttgcag ttcaacgcct ttacggattt
     2041 tttccgccat tagcagacca tcgacaggat tcaccgacgg cactgcaaca gcaaattctt
     2101 cgccgcccat gcgcgccacc agccccttat caccgacaat cttctgaata tgccgggcaa
     2161 acacgcttaa cactttatcg ccacattcat gtccatagtt atcgttaatg cttttgaaat
     2221 agtcgatatc aagcagcatg accgtcagat gttgtgtctg tttcagcgat ggacttttca
     2281 gcgcctcata aagaccggac cgtgagtaaa cctgagtcag aaaatcaaag tcggctcgca
     2341 gcgcaacttg cttcattagc aaattgatcg ctgccacgct aaaagaaacc ataattgggc
     2401 atatcgccat cgtggcaata ccgagacgtg cggagaacat ttccggaatg gagaacggcg
     2461 aaccgaccga gatatcaatc accgaatttg ccaccagtac gatttccacc gcaccggtga
     2521 caaaggtcaa cagacatgtt acctgcggcg tatagcgcac tgcacaccag attaatgcag
     2581 gcagcgggaa cgccagactc cccgctccgc caatgactac cgaggcaata accgacacaa
     2641 taagcgcgat ggcgggcatc atctgttctg ctttaaagcg cggcagcact ccaggaatag
     2701 ccaacgtcag catacaaggc acgatcaaca cgcccgttga gaattgctca ctgaaccagt
     2761 ccgcaagcaa aggccagaaa tccagactat caatactgac cgaaccaatc gccccgacaa
     2821 tagcgcataa taatgcggca atcagacagt aattaaatag ccgtaaagca cttactggct
     2881 catacttatt tttccccaga cgcttatccc gcacgaccag taaggcgacg gtaacaataa
     2941 acatcatatt ggagaaattg ataaccagtg aaaccagtcc ccattcggtg gttatggcat
     3001 cataaaccag catcgccaca taactgatcg catagtaatg cagtcgatta agccagacat
     3061 agcgggcaaa cacccccgcc attacgccat tcagcggcca gaaaagagaa agttcgtgaa
     3121 ccagccgtaa ctctgcacca ataaaataga acagcgtggt tagcgcaaaa atagcgatgc
     3181 tattacgtag cagatgacct tcctggaaca aacggaaagt tgaaatgggg tgtacatgca
     3241 ttaattatct gtccatacaa ttgcgggtgg caattatctc aacgtgataa gtaaattctc
     3301 ataaaggata gcatgttcgc taacagcggc ggcaaataaa ggtaccctgg gaatgctatt
     3361 ttatcgtttt ccgcaattct catggctgat tcatctatac aaccagccag tgatagatta
     3421 ccgccacgat ataacgttgt tgttcttcgc ttaacgaaac accctgcgga atgttatgcc
     3481 acttagccag acaaccgcga cagcaggtcg cggtggcgtg ctgagcgata aacaccggat
     3541 gcccgcgcat gggggtttgc ttaccgtcat tagccggtaa tgccggagca agacgtttag
     3601 cgacaaaatc cgccgcatgt tgttcgatga ctggcgcgcc tttctccagg caatactgac
     3661 gctctttcat gcccagacga aagcgagagc gaaattttga acgggataaa cgcgcgaaga
     3721 gtggatcgag agactgcatt aataaaccga acgccctaac tgtttcgtca attgttcaag
     3781 gacggcgata cccgcaagcg agttacctgc atcatccagt tccggactcc agactgcgat
     3841 ggccatttca tgcggaacaa tcgccacaat accgccacca acgccagatt tcgccggtag
     3901 tcccacccgc caggcaaact cccccgcgtt ctggtacata ccactggtcg ccatcagcgc
     3961 gttaatttgc cgcgcctgca ttggcgtcac cacaggttca tcaatatgaa tagctttccc
     4021 ctgattagcc agaaagacaa acgtccgggc cagctctaca cagctcattt tcagagcgca
     4081 gtaatgaaag tagttttgca gaacggttgt cacgtcatga tggaaattgc caaacgactt
     4141 catcagccag gcgatagccg catttcgcgc ggaatgttca aattcggaac gcgctaccac
     4201 cgtatcgtat gaaatatcag acacaccgct taagccgcgc acgacttcca gcatacgttg
     4261 ccgtggtgcg cttagtcgcc cttgcaacat atcgcagacc accagcgcac cggtattaat
     4321 gaacggatta cgcggaatac cctgttccat ttccagttgc actaaagaat tgaacggtga
     4381 tccagacgga tctttgccga cgcgttgcca gatttcctct tcggagtaat gacgcatggc
     4441 gacaacgaga ctcagcactt tggaaataga ctgaatggaa aaacgttctt gcgcgtctcc
     4501 ggcctgaaaa agctgtccgt caacggtaca gatagcaatc cccaatcggg aaccgtctac
     4561 tgtagccagc gccggaatat aatccgcgac tttaccctga ccaatgagcg gccgcacttg
     4621 ccgcaagatg ttttctaaaa ttgcattatc catggcgact gccactttct actcctggac
     4681 cgcaggtctg aaaagacctg cgagtatatc agagctgaat atgtcgcgtc agatccggtc
     4741 tttccacact gtctggatat tacagaattc gtgtaagccg aaatgggaaa gctcacgacc
     4801 aaagccactc tttttcacgc caccaaaggc cactcgcgcg tcgctggcac aataaccatt
     4861 gataaacacc ccaccgcatt ccagacgtgc cgccatctgt ctggcctgtg tttcgtcggt
     4921 ggtaaaaatg gtcgctgaaa ggccgaactc actatcatta gccagttcca gcgcatgttc
     4981 tgcatctttc gcaatggtga ttgcagcaac ggggccaaac atttcttccc gaaacgcggt
     5041 catttctggg gtaacattcg ccagaaccgt tggcggatag tagttacctg ctccagccat
     5101 cttttccccg cccagtaaca aacgcgcacc ctgcgccagg gttttctcca cctgatgatg
     5161 cagctcatca cgtaaatcaa aacgagccat tggtccgaga gcgttctctt catcacgggg
     5221 atcgcccatt ttcaaggctg ccgcagctga cacaaaacgt tcggtaaatg ccgaagcaat
     5281 tccctcttcg ataataaagc gttttgccgc tgcacaaacc tgtccggtat tctgataacg
     5341 tccggctacc gccgctttaa ccgccagttc cagatcggca tcgttaagca caataaatgg
     5401 atccgaaccg cccagttcca gtacgcattt tttcagtgcc gctccagcac gaacacttcc
     5461 ggtcaccgtg acagcagcaa tgcgcgagtc tttaatcatc tgactgacac cgtcgttgtc
     5521 ggcattcagc cagccatata cgccttgcgg gattcccgca tctttaaaca cttgggcaat
     5581 gagctgagca cagcccatca cattcggcgc atgtttaagt aagtagccgt tacctgcaag
     5641 aatgatggga acagcgccac gcatcacctg cctttgatag ttcttgcagc tcaaatgccc
     5701 tatatagtga tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag
     5761 catcagcaat tacatatctc ttcctgatga ttttcccgct cgtaatattg cacctcaagt
     5821 aaaagaagtt ttaaaggatt ttattgatgc actcagtacc ataatatgtg atgaagagtg
     5881 gcgcacgtct ttaaacacca actcagccac aaaaaagata tttaacaacc ttgataactt
     5941 atcatatatt cagaggactt cttttcgggg taacgacaca ctatacaatg aaaaggttca
     6001 gtttaaactc acttatcctg tcaagaatgg aagacacaaa gaaaatattg aatttcaggt
     6061 agtaataaat ttaagcccta tttatttaga taattttcgc catgatggag aaattaatat
     6121 tttttgcgct cccaacccaa agcctgtcac tatggggcgc gtatttcaga ccggcgtcga
     6181 gcgtgtgctc tttctgttta tgaatgattt tattgaacaa tttccaatga tcaaccttgg
     6241 cgcccccatc aaaagagcgc atacaccaca tattgaaccc ctgcctccgg atcaccatac
     6301 cgctgcagat tacttacgcc agtttgattt gcttgtcctg aattttatct ctcgcggtaa
     6361 ttttgtcata ctcccccgat tatggaataa ctctgaggtt cacagatggt tcgtcaataa
     6421 agatcctaac ttgatcaccg ccattctcga cataacggac agcgaattaa aagaggattt
     6481 attgcaaagc ctgatggatt cattaggttc taacaaacat gtacaacccg aagtctgcat
     6541 ctgcttctta tcccttttag cagaacaaga atctcctcat tttcaggact tgtttttatt
     6601 tttcgccaat atgttattgc actatcacca atttatgaat cccaatgaaa gtgatctgaa
     6661 tgacgtgttg atgccagcat cattaagtga tgataaaatt atcaaacata tggcgcgcag
     6721 gcccctcaaa ctgtttgtaa aaaatgaaac gccgccaaaa gttactcacg aagacctggt
     6781 gaaaaacagg cctcgctccc ctgtcagacc acctataccc gcaaccgcca aaacgccaga
     6841 cctccctgaa cgtcattaaa ccgtgatgtt accgactctc tgactcggaa cagaatcagc
     6901 gtcagagaaa cggaaaacgc gatccagatc acaaatgcat tgtattcaca tcattaaccg
     6961 ttttaagatc atttcatcac tttttcgcaa ctcacccgat aatctgttat gacaacaaac
     7021 actgtttccc gcaaagtggc gtggctacgg gtcgttacgc tggcagtcgc cgccttcatc
     7081 ttcaacacca ccgaatttgt ccctgttggc ctgctctctg acattgcgca aagttttcac
     7141 atgcaaaccg ctcaggtcgg catcatgttg accatttacg catgggtagt agcgctaatg
     7201 tcattgcctt ttatgttaat gaccagtcag gttgaacggc gcaaattact gatctgcctg
     7261 tttgtggtgt ttattgccag ccacgtactg tcgtttttat cgtggagctt taccgttctg
     7321 gtgatcagtc gcattggtgt ggcttttgca catgcgattt tctggtcgat tacggcgtct
     7381 ctggcgatcc gtatggctcc ggccgggaag cgggcacagg ccttgagttt aattgccacc
     7441 ggtacagcac tggcgatggt cttaggttta ccgctcgggc gcattgtggg gcagtatttc
     7501 ggctggcgaa tgaccttctt cgcgattggt attggggcgc ttgttaccct tttgtgcctg
     7561 attaagttac ttcccttact gcctagtgag cattccggtt cattgaaaag cctcccgcta
     7621 ttgttccgcc gcccggcatt gatgagcatt tatttgttaa ctgtggtggt tgtcaccgcc
     7681 cattacacgg catacagcta tatcgagcct tttgtgcaaa acattgcggg attcagcgcc
     7741 aactttgcca cggcattact gttattactc ggtggtgcgg gcattattgg cagcgtgatt
     7801 ttcggtaaac tgggtaatca atatgcgtct gcgttggtaa gtacggcgat tgcgctgttg
     7861 ctggtgtgtc tggcactgct gctacctgcg gcgaacagtg aaatacacct cggggtgctg
     7921 agtattttct gggggatcgc gatgatgatc atcgggcttg gtatgcaggt taaagtgctg
     7981 gcgctggcac cagatgctac cgacgtcgcg atggcgctat tctccggcat atttaatatt
     8041 ggaatcgggg cgggtgcgtt ggtaggtaat caggtgagtt tgcactggtc aatgtcgatg
     8101 attggttatg tgggcgcggt gcctgctttt gctgcgttaa tatggtcaat cattatattt
     8161 cgccgctggc cagtgacact cgaagaacag acgcaatagt tgaaaggccc attcgggcct
     8221 tttttaatgg tacgttttaa tgatttccag gatgccgtta ataataaact gcacacccat
     8281 acataccagc aggaacccca tcaggcggga gatcgcttca atgccaccct tgcccaccag
     8341 ccgcataatt gcgccggagc tgcgtaggct tccccacaaa ataaccgcca ccaggaaaaa
     8401 gatcagcggc ggcgcaacca tcagtaccca atcagcgaag gttgaactct gacgcactgt
     8461 ggacgccgag ctaataatca tcgctatggt tcccggaccg gcggtacttg gcattgccag
     8521 tggtacaaag gcaatattgg cactgggttc atcttccagc tcttccgact tgcttttcgc
     8581 ctccggtgaa tcaatcgctt tctgttgcgg aaaaagcatc cgaaaaccga taaacgcgac
     8641 gattaagccg cctgcaattc gcagaccggg aatcgaaatg ccaaatgtat ccatcaccag
     8701 ttgcccagcg taatacgcca ccatcatgat ggcaaatacg tacaccgagg ccatcaacga
     8761 ctgacgatta cgttcggcac tgttcatgtt gcctgccagg ccaagaaata gcgcgacagt
     8821 tgttaatggg ttagctaacg gcagcaacac caccagcccc aggccaattg ctttaaacaa
     8881 atctaacatt ggtggttgtt atcctgtgta tctgggttat cagcgaaaag tataaggggt
     8941 aaacaaggat aaagtgtcac tctttagcta accttgcatc gcattgacca aaacttgaac
     9001 cgatttagca aaacgtggca tcggtcaatt cattcatttg acttatactt gcctgggcaa
     9061 tattaccccc tacaactaat tacttgccag ggcaactaat gtgaaaagta ccagcgatct
     9121 gttcaatgaa attattccat tgggtcgctt aatccatatg gttaatcaga agaaagatcg
     9181 tctgcttaac gagtatctgt ctccgctgga tattaccgcg gcacagttta aggtgctctg
     9241 ctctatccgc tgcgcggcgt gtattactcc ggttgaactg aaaaaagtgt tgtcggtcga
     9301 cctgggagca ctgacccgta tgctggatcg cctggtctgt aaaggctggg ttgaaaggtt
     9361 gccgaacccg aatgataagc gcggcgtact ggtaaaactt accaccagcg gcgcggaaat
     9421 atgcgaacaa tgccatcaat tagttggcca ggacctgcat caagaattaa caaaaaacct
     9481 gacggcggac gaagttgcaa cacttgagca tttgcttaag aaagtcctgc cgtaaacaaa
     9541 aaagaggtat gacaatgtcc agacgcaata ctgacgctat taccattcat agcattttgg
     9601 actggatcga ggacaacctg gaatcgccac tgtcactgga gaaagtgtca gagcgttcgg
     9661 gttactccaa atggcacctg caacggatgt ttaaaaaaga aaccggtcat tcattaggcc
     9721 aatacatccg cagccgtaag atgacggaaa tcgcgcaaaa gctgaaggaa agtaacgagc
     9781 cgatactcta tctggcagaa cgatatggct tcgagtcgca acaaactctg acccgaacct
     9841 tcaaaaatta ctttgatgtt ccgccgcata aataccggat gaccaatatg cagggcgaat
     9901 cgcgcttttt acatccatta aatcattaca acagctagtt gataacgtga caacgtcact
     9961 gaggcaatca tgaaaccact ttcatccgca atagcagctg cgcttattct cttttccgcg
    10021 cagggcgttg cggaacaaac cacgcagcca gttgttactt cttgtgccaa tgtcgtggtt
    10081 gttcccccat cgcaggaaca accaccgttt gatttaaatc acatgggtac tggcagtgat
    10141 aagtcggatg cgctcggcgt gccctattat aatcaacacg ctatgtagtt tgttctggcc
    10201 ccgacatctc ggggcttatt aacttcccac ctttaccgct ttacgccacc gcaagccaaa
    10261 tacattgata tacagcccgg tcataatgag caccgcacct aaaaattgca gacccgttaa
    10321 gcgttcatcc aacaatagtg ccgcacttgc cagtccgact acgggcacca gtaacgataa
    10381 cggtgcaacc cgccaggttt catagcgtcc cagtaacgtc ccccagatcc cataaccaac
    10441 aattgtcgcc acaaacgcca gatacatcag cgacaagatg gtggtcatat caatagtaac
    10501 cagactgtga atcatggatg cggaaccatc gaaaatcagc gagacaacaa agaagggaat
    10561 gattgggatt aaagcgctcc agattaccag cgacatcacc gccggacgcg ttgagtgcga
    10621 catgatcttt ttattgaaga tgttgccaca cgcccaacta aatgctgcag ccagggtcaa
    10681 cataaagccg agcatcgcca catgctgacc gttcagacta tcttcgatta acaccagtac
    10741 gccaaaaatc gctaaggcga tccccgccaa ttgtttgcca tgcagtcgct ccccgaaagt
    10801 aaacgcgcca agcacgatag taaaaaacgc ctgtgcctgt aacaccagcg aagccagtcc
    10861 agcaggcata ccaaagttaa tggcgcagaa cagaaaggca aactgcgcaa aactgatggt
    10921 taatccatac cccaagagca aattcagtgg tactttcggt cgtgcaacaa aaaagatggc
    10981 cggaaaagcg accagcataa agcgcaaacc ggccagcatc agcggtggca tgttatgaag
    11041 ccccactttg atgaccacaa aatttagccc ccatacgacc actaccagta gcgccaacac
    11101 cccatctttt cgcgacattc taccgcctct gaatttcatc ttttgtaagc aatcaactta
    11161 gctgaattta cttttcttta acagttgatt cgttagtcgc cggttacgac ggcattaatg
    11221 cgcaaataag tcgctatact tcggattttt gccatgcgtc cgatggttaa taacaacgac
    11281 gaggcaagaa gggcgctggt agagcgtcgt agggataagt tcatttgtct gtccggcagt
    11341 gcgtttcgtt atgttttatt ttagagatgt aaagaaatag catggctacg ctgccattta
    11401 tgaccattta cttgagtcgc cagtacagcc tgagtgtcga tctaatcggt tatgcgatga
    11461 caattgcgct cactattggc gtcgttttta gcctcggttt tggtatcctg gcggataagt
    11521 tcgacaagaa acgctatatg ttactggcaa ttaccgcctt cgccagcggt tttattgcca
    11581 ttcctttagt gaataacgtg acgctggttg tgctcttttt tgccctcatt aactgcgcct
    11641 attctgtttt tgctaccgtg ctgaaagcct ggtttgccga caatctttcg tccaccagca
    11701 aaacgaaaat cttctcaatc aactacacca tgctaaacat tggctggacc atcggtccgc
    11761 cgctcggcac gctgttggtg atgcagagca tcaatctgcc cttttggctg gcagctatct
    11821 gttccgcgtt tcccatgctt ttcattcaaa tatgggtaaa gcgcagcgag aaaatcatcg
    11881 ccacggaaac aggcagtgtc tggtcgccga aagttttatt acaagataaa gcactgttgt
    11941 ggtttacctg ctctggtttt ctggcttctt ttgtaagcgg cgcatttgct tcatgcattt
    12001 cacaatatgt gatggtgatt gctgatgggg attttgccga aaaggtggtc gcggttgttc
    12061 ttccggtgaa tgctgccatg gtggttacgt tgcaatattc cgtgggccgc cgacttaacc
    12121 cagctaacat ccgcgcgctg atgacagcag gcaccctctg tttcgtcatc ggactggtcg
    12181 gttttatttt ttccggcaac aacctgctat tgtggggtat gtcagctgcg gtatttactg
    12241 tcggtgaaat catttatgcg ccgggcgagt atatgttgat tgaccatatt gcgccgccag
    12301 gaatgaaagc cagctatttt tccgcccagt ctttaggctg gcttggtgcc gcgattaacc
    12361 cattagtgag tggcgtagtg ctaaccagcc tgccgccttc ctcgctgttt gtcatcttag
    12421 cgttggtgat cattgctgcg tgggtgctga tgttaaaagg gattcgtgca agaccgtggg
    12481 ggcagcccgc gctttgttga tttaagtcga acacaataaa aatttaattc agccttcgtt
    12541 taggttatct ctgctaatat ctttctcatt gagatgaaaa ttaaggtaag cgaggaaaca
    12601 caccacacca taaacggagg caaataatgc tgggtaatat gaatgttttt atggccgtac
    12661 tgggaataat tttattttct ggttttctgg ccgcgtattt cagccacaaa tgggatgact
    12721 aatgaacgga gataatccct cacctaaccg gccccttgtt acagttgtgt acaaggggcc
    12781 tgatttttat gacggcgaaa aaaaccgcca gtaaaccggc ggtgaatgct tgcatggata
    12841 gatttgtgtt ttgcttttac gctaacaggc attttcctgc actgataacg gatcgttgac
    12901 acagtagcat cagttttctc aatg
//
LOCUS       DAJTRB010000106        12652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTRB010000106 DAJTRB010000000
VERSION     DAJTRB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.106"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            118..1815
                     /gene="maeA"
                     /locus_tag="OO330_003396"
     CDS             118..1815
                     /gene="maeA"
                     /locus_tag="OO330_003396"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5884148.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1949..2959
                     /gene="adhP"
                     /locus_tag="OO330_003397"
     CDS             1949..2959
                     /gene="adhP"
                     /locus_tag="OO330_003397"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5884149.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASMAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKLVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3105..3389
                     /locus_tag="OO330_003398"
     CDS             3105..3389
                     /locus_tag="OO330_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5884150.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO330_003399"
     CDS             complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO330_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5884151.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO330_003400"
     CDS             complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO330_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5884152.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO330_003401"
     CDS             complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO330_003401"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7620..7622),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5884153.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8439..9320
                     /gene="yddG"
                     /locus_tag="OO330_003402"
     CDS             8439..9320
                     /gene="yddG"
                     /locus_tag="OO330_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5884154.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9575..10675
                     /gene="ompC"
                     /locus_tag="OO330_003403"
     CDS             9575..10675
                     /gene="ompC"
                     /locus_tag="OO330_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5884155.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10839..11324
                     /locus_tag="OO330_003404"
     CDS             10839..11324
                     /locus_tag="OO330_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5884156.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11434..12423
                     /locus_tag="OO330_003405"
     CDS             11434..12423
                     /locus_tag="OO330_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5884157.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3331 a         3128 c         2943 g         3250 t
ORIGIN      
        1 accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac gccctggcgg
       61 taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt gagtgacatg
      121 gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc tgtactgctg
      181 gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg taacttcaac
      241 ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga acgagcatgg
      301 atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg taacatccag
      361 gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga gatgatgcct
      421 gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt
      481 tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat tctgcaaaac
      541 gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat tctggggctt
      601 ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct ctataccgcc
      661 tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg aacgaacaac
      721 caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat cactgacgat
      781 gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg gccggacgtg
      841 ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa ccgctatcgc
      901 aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac agtcggcaca
      961 ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt
     1021 ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa
     1081 ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt tggcctgctg
     1141 acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa gcgcgaaaac
     1201 ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt gcgcaatgta
     1261 aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga agagatcatc
     1321 cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa cccgacgtct
     1381 cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc gctggtcgcc
     1441 actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat cgcccagtgt
     1501 aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg cgcgtcacgt
     1561 atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc gccgctggtc
     1621 ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt ctcccgcgca
     1681 attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa aacgtctgct
     1741 gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg cgactaccgc
     1801 cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt ccctctccct
     1861 ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct cacctgctat
     1921 gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga aggatcatca
     1981 tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc tgctgaaaat
     2041 ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt ttggtgacaa
     2101 aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg gcccaggtgt
     2161 cacctcatta aaaccaggcg atcgtgccag catggcgtgg ttttacgaag gatgcggtca
     2221 ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa atgccggata
     2281 cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg cggtaaaagt
     2341 gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg tcaccaccta
     2401 caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct acggtcttgg
     2461 cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc
     2521 cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag atttagcgat
     2581 taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg gcgctcacgc
     2641 tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc
     2701 aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg atatcccacg
     2761 tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac
     2821 cgaagccttc cagtttgccg ccgaaggcaa actggtgccg aaagtcgccc tgcgtccgtt
     2881 agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt
     2941 gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt cgcagcatcg
     3001 cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg aacaataatg
     3061 atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa atggccaatc
     3121 atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc agcctgcgcg
     3181 agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg accggaaaag
     3241 cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa
     3301 tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg gtggttgtgt
     3361 cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg ccatttgaaa
     3421 ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca cgttcacttc
     3481 aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc atccgggatg
     3541 aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc attttcagct
     3601 taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct gccttctcga
     3661 tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc ttcccttcga
     3721 tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc aggatggcgt
     3781 ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg
     3841 ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc agcacgaaaa
     3901 tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta ccaacatccg
     3961 ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac ggaatatctt
     4021 tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa
     4081 tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg
     4141 tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc
     4201 aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg
     4261 acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc aataccgatg
     4321 tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac
     4381 gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag accgtgatac
     4441 agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc gaccccttcc
     4501 gggttgtaga cgccagcatg ttcgtaacca cgcgccttca gtttcgccac gcgctgttcc
     4561 gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc
     4621 acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc
     4681 cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca
     4741 cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca
     4801 ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca gccgtcttta
     4861 cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat caccgtccag
     4921 gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca
     4981 cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat acaggtggaa
     5041 acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg
     5101 gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg ttcgccccct
     5161 tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga gtttgcatca
     5221 ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc
     5281 cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag cggtttcaga
     5341 cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac agtgacacga
     5401 tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac aaactgttcc
     5461 ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg ctcggtcaga
     5521 cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag cgcgtcttgt
     5581 tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt agtgcccagc
     5641 ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca
     5701 aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt acccggtgcg
     5761 gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca ctggatcagc
     5821 atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt gtagagtacg
     5881 cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc ggacgggtcg
     5941 gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt gtagatccag
     6001 caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct
     6061 ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata gccgttgttc
     6121 tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca gctcatcttc
     6181 atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag atgatggtag
     6241 atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag
     6301 tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc aaagcaggtc
     6361 gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt cgactcgccg
     6421 tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc
     6481 agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac cgggttaaag
     6541 ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata cttgatgacg
     6601 tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc tttctgcgcg
     6661 gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt gctccagtag
     6721 ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca
     6781 acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag cagacctaag
     6841 tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata
     6901 cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg cgcacccacg
     6961 gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg
     7021 gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca gatgttttca
     7081 acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac acagcgcgga
     7141 tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata gttccaggac
     7201 gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc ttcgaaagca
     7261 aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt
     7321 ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc cgtaccggaa
     7381 cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg atcgacaacg
     7441 atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc
     7501 gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat atccacccag
     7561 tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt
     7621 cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttctgggtc
     7681 agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga aagccaacgg
     7741 tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc agctttcatc
     7801 agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct
     7861 ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc cagcaaaccg
     7921 gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga
     7981 taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa tagcccgcaa
     8041 cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa tttgtagttt
     8101 cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt tgttccagcc
     8161 ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt
     8221 tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa
     8281 atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa
     8341 tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct ataaaattca
     8401 gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc
     8461 tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca
     8521 gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt
     8581 taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg
     8641 ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga
     8701 cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc
     8761 tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat
     8821 tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa
     8881 tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta
     8941 tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg
     9001 tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag
     9061 aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat
     9121 ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag
     9181 gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc
     9241 tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc
     9301 tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa
     9361 attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc
     9421 gacttcgatc acatatcgat aaataatatt attctgtttt tctcattgtt tttaatcgcc
     9481 attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa
     9541 atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt
     9601 tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg
     9661 taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg
     9721 tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga
     9781 tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa
     9841 cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg
     9901 ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat
     9961 gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac
    10021 caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt
    10081 tgccgcgcaa tatcaaggca aaaatgaacg tactgacaac agtcatcttt atggtgctga
    10141 ctacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt
    10201 tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc
    10261 taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga
    10321 tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg
    10381 ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga
    10441 cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta
    10501 cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa
    10561 tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc
    10621 cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc
    10681 tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt
    10741 aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat
    10801 atcctccggg gacatcttac tataatattt attgatgagt gattatgacc aatattaata
    10861 cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa
    10921 ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg
    10981 gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat
    11041 ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata
    11101 cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg
    11161 ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac
    11221 tggaaaacat ttatattgat cataacaatc tggaaagttt agatttaaaa aatcagcatt
    11281 cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat
    11341 aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa
    11401 gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat
    11461 catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa
    11521 aacactacaa aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac
    11581 attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat
    11641 gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat
    11701 gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac
    11761 ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat
    11821 agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc
    11881 agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata
    11941 gataggcttt catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa
    12001 ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt
    12061 gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa actaacaagt
    12121 ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct
    12181 gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac
    12241 cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga
    12301 ctggacaata ataaaataac ccacattgat actaataata catccgatat tggaacttta
    12361 ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat
    12421 tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact
    12481 ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat
    12541 aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat acacacttac
    12601 aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac ct
//
LOCUS       DAJTRB010000107        12647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTRB010000107 DAJTRB010000000
VERSION     DAJTRB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.107"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..1040
                     /locus_tag="OO330_003406"
     CDS             <1..1040
                     /locus_tag="OO330_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5884158.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1041..3206
                     /locus_tag="OO330_003407"
                     /pseudo
     CDS             1041..3206
                     /locus_tag="OO330_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
     gene            3220..4131
                     /locus_tag="OO330_003408"
     CDS             3220..4131
                     /locus_tag="OO330_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5884159.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4131..5426
                     /locus_tag="OO330_003409"
     CDS             4131..5426
                     /locus_tag="OO330_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5884160.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSISGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5471..5701
                     /locus_tag="OO330_003410"
     CDS             5471..5701
                     /locus_tag="OO330_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884161.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5679..6179
                     /locus_tag="OO330_003411"
     CDS             5679..6179
                     /locus_tag="OO330_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5884162.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLIATARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6179..7597
                     /locus_tag="OO330_003412"
     CDS             6179..7597
                     /locus_tag="OO330_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5884163.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIVSASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7597..8445
                     /locus_tag="OO330_003413"
     CDS             7597..8445
                     /locus_tag="OO330_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884164.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8445..8900
                     /locus_tag="OO330_003414"
     CDS             8445..8900
                     /locus_tag="OO330_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5884165.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSGTREELIDFLNHGR"
     gene            8903..9595
                     /locus_tag="OO330_003415"
     CDS             8903..9595
                     /locus_tag="OO330_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5884166.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9605..10936
                     /locus_tag="OO330_003416"
                     /pseudo
     CDS             9605..10936
                     /locus_tag="OO330_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
     gene            10937..>12647
                     /locus_tag="OO330_003417"
     CDS             10937..>12647
                     /locus_tag="OO330_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5884167.1"
                     /translation="MVNYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPRTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQAPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIE"
BASE COUNT         3401 a         3125 c         3334 g         2787 t
ORIGIN      
        1 ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg
       61 aagatggcat ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg
      121 gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag
      181 gtcttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg
      241 ggatgtctga cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca
      301 acatgaccat ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat
      361 catatcccga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc
      421 gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact
      481 tctacgtaat tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt
      541 ggtgggataa ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa
      601 cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc
      661 ctcatgatgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag
      721 acgctggatt tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg
      781 agccaggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata
      841 cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg
      901 tcaagcttaa cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg
      961 caaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc
     1021 ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt
     1081 ttgacgcaga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt
     1141 ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg
     1201 gacaattcga tgttgttcgc ccgggggtca ggaaactggt cgcagagatg cgccggaacc
     1261 ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga
     1321 tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg
     1381 ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca
     1441 acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac
     1501 acgtcatatg agacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg
     1561 tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc
     1621 cggataccct gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga
     1681 atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca
     1741 tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg
     1801 tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc
     1861 ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag
     1921 ccggagagca tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg
     1981 agtgctacga gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta
     2041 tgtcgttcaa cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg
     2101 agcaaatcga aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc
     2161 agaacaggac tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa
     2221 accctgaagt gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag
     2281 aagtggctag tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg
     2341 tcaatcaact gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta
     2401 ccgcaatgcg acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc
     2461 ctcgtcatgt aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc
     2521 aagttgtcga ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg
     2581 agtgctatac agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga
     2641 ttcaggagtt gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc
     2701 aatacttcac gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc
     2761 aactggtgat gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc
     2821 aggcacaaca acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc
     2881 tgcaaggtca ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag
     2941 ctgccaaggt tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca
     3001 atatggacct cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc
     3061 agcaacagcg cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gatgcagacc
     3121 agactcattc acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg
     3181 gcggagtagc cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt
     3241 caggcttctg aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta
     3301 gttgtcgata atgccaacga cagcgcaggt caggaagaag gctttgagat tgtcctgaaa
     3361 gacgatgaga aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc
     3421 gaacgcaaac gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg
     3481 ccggagcacc tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt
     3541 tccgaagatg cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag
     3601 caggcaaaca gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag
     3661 cagggtcgca aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt
     3721 aagcactacg aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca
     3781 ttcatgcaac tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa
     3841 tccgccgctc tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg
     3901 atggacgggc aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag
     3961 cctcgagcca agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct
     4021 gttgctgcaa atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat
     4081 gtagagacgt accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa
     4141 atgaaggtca actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga
     4201 cgccaatggc gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa
     4261 gcaacaccgt gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa
     4321 ctggcaacgc aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata
     4381 acgatttctt cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca
     4441 tccaggcatc cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa
     4501 ctgaaatggg ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc
     4561 ctggctggga ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg
     4621 acatgggcat cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg
     4681 tagatggtga catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc
     4741 gtcagattgc tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt
     4801 caaccgcgac tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc
     4861 ttgataccga tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct
     4921 ccaccaccgg atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc
     4981 agatggcgaa gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg
     5041 gtactcacat cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag
     5101 aagagaaggc ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc
     5161 tgaacgtggc aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt
     5221 ctcagccgat cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca
     5281 tttcaggtat tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg
     5341 gtaagtgccg tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg
     5401 ttggtctgcc taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct
     5461 ggagacaagc atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt
     5521 tggctacgac tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg
     5581 gttttcacat cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca
     5641 gcgtaaaaag cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg
     5701 atttagttct tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg
     5761 tcgaaccgca gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc
     5821 ttggcggtga tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg
     5881 ctccggatcc gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca
     5941 accttgcctg ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttatagcca
     6001 ctgccagata cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag
     6061 ctaaatgtaa gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga
     6121 agtggaatgg ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat
     6181 gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta
     6241 tatcgactat ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg
     6301 atatcttcgc tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg
     6361 cgtcgagtac aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa
     6421 aggagaaagt gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg
     6481 gacatcacag gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt
     6541 caaaaccgtc tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt
     6601 tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg
     6661 gtttatcact gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc
     6721 cgagtctcag cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt
     6781 tggttcatcg actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt
     6841 gtatgttgca caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac
     6901 gccattcgct gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta
     6961 tatcatcgac tccggtcagg tgtcaccaat tgtcagcgcg agcattgaga aaatcctccg
     7021 ctcctacact gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca
     7081 tgagttgctg attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaagcgc
     7141 caatggccca caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat
     7201 cgacttcgtt tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg
     7261 gaaactgcaa ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac
     7321 tccactcttc aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg
     7381 cgttgcgcag tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg
     7441 gcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa
     7501 gcgagtaggg cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc
     7561 tgtcactctg tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg
     7621 ccggtaatca tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg
     7681 cagtcgtatc tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa
     7741 gccaacgagg ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg
     7801 attctcgctg accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag
     7861 gagcgaatca gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat
     7921 gaaagcgata tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac
     7981 catgaaacgc gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt
     8041 tactctggtg tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa
     8101 acgctcacac cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg
     8161 gttgtgttca acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc
     8221 agcggaaccg ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg
     8281 gtcagcagtc gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc
     8341 gtggataaag acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt
     8401 gcggcgttta ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac
     8461 caacgcgaaa catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga
     8521 gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc
     8581 acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc
     8641 acgccatgta cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc
     8701 gatacattct gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc
     8761 gacacggtca gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat
     8821 acttcaaagg cgtggatgac gtgacttttt acagcggcac acgcgaagaa ctaatcgact
     8881 tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg
     8941 cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa
     9001 gtatgcagca gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat
     9061 catgaaaaac cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa
     9121 cctttccagt ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta
     9181 taaagacctt gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg
     9241 acttggttcg acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc
     9301 ttggttatca aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct
     9361 gcaaggtcag gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca
     9421 aagcgcagct cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag
     9481 cggtggcacg tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac
     9541 gccttggggc gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggt
     9601 aatcatggct acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa
     9661 ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa
     9721 tgatattgag cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg
     9781 tattgcgggt gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc
     9841 gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata
     9901 tccagaccag attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa
     9961 ttctatcggc accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca
    10021 atcatggctg caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt
    10081 cgctcagatg taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat
    10141 gaacagtctc gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt
    10201 taccatgcgc ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat
    10261 gcgcggacaa gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac
    10321 aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga
    10381 cctgaagcgc caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa
    10441 gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt
    10501 cactctgaac gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt
    10561 aatccccaac gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa
    10621 atcacaagca tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc
    10681 cgagggcaaa aagctcgact aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc
    10741 ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt
    10801 tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga
    10861 ttacctgaag cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc
    10921 atctttgggg cagtaaatgg ttaattaccg tgatttgtta gagcaggctg gcgcacgtta
    10981 cggtgtgcca gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc
    11041 cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc atgcctagga catggcgtga
    11101 tatgggttat accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg
    11161 ctatctggca aagatgtacc agcagtttgg taactggcgt gacgctcttc aggcttatca
    11221 cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg
    11281 ccgcggttac gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac
    11341 agtcgaacag cgcgccacct ctgcaaaggt acatcctcag caagccccta acaacccgtt
    11401 tgcacaactg gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc
    11461 aaataatcca tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt
    11521 aagttctgtc gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc
    11581 aacacgggaa gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag
    11641 tgcagggcaa attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt
    11701 gcagggtggc gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt
    11761 ggatgatgtt tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc
    11821 aataggcggt tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct
    11881 cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact
    11941 tagcattgct acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa
    12001 tataattagt ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc
    12061 accattctcc ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag
    12121 agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac ccagaggaag tcgttcgcac
    12181 agtagcagca caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc
    12241 tcaggctgaa gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca
    12301 cttttccggg aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc
    12361 tgctctacag gtgcaggaaa atgaagcaat caggcagcta gcacagggcg cgggtgagat
    12421 aattgatcgc gtttccggtg caaaagatgc tcttggtatg agcgacaagt ttattgatac
    12481 ggtcaatgga agaatgtctg cgctgatgaa acgaagcgac cagctttatc gcaatgttga
    12541 aaaggcgatg cctgcaggtg caaaaattga tgcgccatca acaaggtcaa tgctcaaaca
    12601 ggtggcagaa gatcttggcg ggatgaaaaa ccttgaccct attgaaa
//
LOCUS       DAJTRB010000108        12627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTRB010000108 DAJTRB010000000
VERSION     DAJTRB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.108"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(105..743)
                     /gene="rutR"
                     /locus_tag="OO330_003418"
     CDS             complement(105..743)
                     /gene="rutR"
                     /locus_tag="OO330_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5884168.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1031..2122
                     /gene="rutA"
                     /locus_tag="OO330_003419"
     CDS             1031..2122
                     /gene="rutA"
                     /locus_tag="OO330_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5884169.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2122..2814
                     /gene="rutB"
                     /locus_tag="OO330_003420"
     CDS             2122..2814
                     /gene="rutB"
                     /locus_tag="OO330_003420"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5884170.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2826..3212
                     /gene="rutC"
                     /locus_tag="OO330_003421"
     CDS             2826..3212
                     /gene="rutC"
                     /locus_tag="OO330_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5884171.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3220..3966
                     /gene="rutD"
                     /locus_tag="OO330_003422"
     CDS             3220..3966
                     /gene="rutD"
                     /locus_tag="OO330_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5884172.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVGMQLALDYPASVTMLVSVNGWLRINAH
                     TRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAHFQGKNNL
                     LRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSSELHAALPDSQKMVMPYGG
                     HACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            3976..4566
                     /locus_tag="OO330_003423"
     CDS             3976..4566
                     /locus_tag="OO330_003423"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5884173.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4577..5071
                     /gene="rutF"
                     /locus_tag="OO330_003424"
     CDS             4577..5071
                     /gene="rutF"
                     /locus_tag="OO330_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5884174.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5092..6420
                     /gene="rutG"
                     /locus_tag="OO330_003425"
     CDS             5092..6420
                     /gene="rutG"
                     /locus_tag="OO330_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5884175.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFTQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6502..6609)
                     /locus_tag="OO330_003426"
     CDS             complement(6502..6609)
                     /locus_tag="OO330_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884176.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            6982..7578
                     /gene="wrbA"
                     /locus_tag="OO330_003427"
     CDS             6982..7578
                     /gene="wrbA"
                     /locus_tag="OO330_003427"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5884177.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7599..7826
                     /locus_tag="OO330_003428"
     CDS             7599..7826
                     /locus_tag="OO330_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5884178.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7864..9105)
                     /gene="agp"
                     /locus_tag="OO330_003429"
     CDS             complement(7864..9105)
                     /gene="agp"
                     /locus_tag="OO330_003429"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5884179.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9398..10654)
                     /locus_tag="OO330_003430"
     CDS             complement(9398..10654)
                     /locus_tag="OO330_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5884180.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10915..11835
                     /gene="cbpA"
                     /locus_tag="OO330_003431"
     CDS             10915..11835
                     /gene="cbpA"
                     /locus_tag="OO330_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5884181.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11835..12140
                     /gene="cbpM"
                     /locus_tag="OO330_003432"
     CDS             11835..12140
                     /gene="cbpM"
                     /locus_tag="OO330_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5884182.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12233..>12627)
                     /gene="torD"
                     /locus_tag="OO330_003433"
     CDS             complement(12233..>12627)
                     /gene="torD"
                     /locus_tag="OO330_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5884183.1"
                     /translation="ARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGM
                     ETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAAR
                     CRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3027 a         3224 c         3170 g         3206 t
ORIGIN      
        1 aacgttcgct gagtgtgaat accgctgccg ctggcggtaa cgccagttta atgacgatcg
       61 gttaaacagg tagccggagg atgttacaac ctcctccgtc atctttaacg tggtcgaatc
      121 ccctcaataa taatccgctg cacgttttca accgtttgat tgaaaaatac ctcatcgcgc
      181 aacgtcgcgc ctgtcaccgc ctccacctga ggggcgaaat cggcgtaatg ttgagtggaa
      241 gcccaaatca taaaaatcaa atgctgcgga tcaatcggcg cgagtttgcc gcttttgacc
      301 caaccggcaa tcagcgccga tttctcatca attaatgcct tcaaatcgcc cgtcagttca
      361 tccattaaca acggcgcgcc tgccagcatc tccatacaga acaggcgcga agcctgcgga
      421 taatcgcgtg agacttccag cttcagacgg atgtactctt ttatcgccgc cagcggggcg
      481 aaatcttcac gaaacgcttt taacggtgcc agccagatat cgagaatctg ccgcagcacg
      541 gcaatataca gcgcctcttt tgacgggaag taatacagca gattggtttt tgaaacaccc
      601 gccaactctg cgatctgctc cagtcttgtg ccgtgaaaac cgaattgtga aaaagtgtcc
      661 agtgctgcgc taagaatcgc ttttttcttc gcgcttactg cgcgcgaacg tttacccgtt
      721 gttttcactg cgccttgcgt catgcgctct cccctcttgt agattggtaa caggatagca
      781 aaacccccgc ccggcctcga ccttctgcac tctcatcgcg cacggatgca tgttttatgt
      841 gcaactgttt tgaccgttta gtccactttt taccagatat tttagataac acaatgctaa
      901 cacattaata acattcactt tttaaaaact ggcatccgct ttgcaaacaa gccaatacgc
      961 ggctgaaaag gatatgcagg atgcagcgcc ccgtctgact ttcactttac gagacgaaga
     1021 gaggttgatg atgaaaattg gcgtattcgt acctattggc aacaacggct ggctcatttc
     1081 gacccacgcg ccgcagtaca tgccgacctt tgaactgaat aaagccattg cgcaaaaagc
     1141 ggagcattac catttcgatt tcgccctgtc gatgatcaaa ctgcgtggct ttggcggcaa
     1201 aactgagttc tgggatcaca accttgagtc gttcaccttg atggcgggtc tggcagccgt
     1261 gacctcgcgc attcagattt acgccactgc tgccacctta acgttacctc cggcaatcgt
     1321 cgcccgtatg gccgcaacca tcgactccat ctctggcggg cgttttggcg tcaacctcgt
     1381 gactggctgg caaaagcccg agtatgagca gatgggtatc tggcctggcg atgactattt
     1441 ctcccgtcgt tacgactatc tcaccgaata tgttcaggtg ctgcgcgacc tgtggggcac
     1501 ggggaaaagc gattttaaag gcgatttttt caccatgaat gattgtcgcg tcagtccgca
     1561 accgagtatc cccatgaaag tgatctgcgc cgggcaaagc gacgctggca tggcgttctc
     1621 cgcccagtat gccgatttca acttctgttt cggcaaaggc gtaaatacac ccacggcttt
     1681 cgccccgacc gctgcgcgga tgaaacaggc cacagagcaa accggacgcg acgttggctc
     1741 ttatgtgttg tttatggtga ttgccgatga aaccgacgat gccactcgcg ccaaatggga
     1801 acactacaaa gcgggcgcgg atgaagaggc gttaagctgg ctaaccgaac aaagtcagaa
     1861 agatacccgc tccggtactg acaccaacgt tcgtcagatg gccgatccca cttcggcggt
     1921 aaacatcaat atggggacgt tagtcggttc ttacgccagt gtcgcgcgca tgttagatga
     1981 agtcgcaagc gtgcctggtg cagaaggcgt gctgttgacc ttcgatgatt ttctgtcggg
     2041 aatcgaaacc ttcggcgagc gcattcaacc actgatgcag tgccgcgccc atctctctgc
     2101 gctgactcag gaggtggcat gatgacaacc ttaaccgctc gaccggaagc cattaccttc
     2161 gatccgcagc aaagtgcgca gatcgtggtg gatatgcaaa acgcttatgc cacgccaggc
     2221 ggctacttag atctcgccgg gtttgatgtc tcaaccactc gcccggtcat tgccaacatt
     2281 caaaccgctg tgaccgcagc gcgagcggca gggatgttga tcatctggtt tcaaaatggc
     2341 tgggatgaac agtatgtcga ggctggcggc cccggctcac cgaattttca taaatcgaac
     2401 gccctgaaaa ccatgcgtaa gcagccgcag ctgcagggga aattgctggc gaaaggctcc
     2461 tgggattatc aactggtgga tgaactggtg ccgcagcctg gcgatattgt gctgccgaag
     2521 ccgcgctaca gcggtttctt caatacgccg ctggacagca ttttacgcag ccgtggaata
     2581 cgccatctgg ttttcaccgg tatcgctacc aacgtctgcg tcgaatcgac gctacgcgat
     2641 ggcttttttc tggagcattt cggcgtggtg ctggaagacg caacacacca ggcggggccg
     2701 gaatttgcac agaaagccgc gttgttcaat atcgaaacct tttttggctg ggtcagcgac
     2761 gtcgaaacgt tctgcgacgc gctttctccc acgtcctttg ctcgtatcgc ttaaggagtt
     2821 tagcgatgcc aaaatccgta attattcccg ctggcagcag cgcaccgctg gcccccttcg
     2881 ttcccggcac gctggctgat ggcgtggtgt atgtctccgg tacgctggct tttgatcaac
     2941 ataataacgt gctgtttgcc gatgacccaa aggcgcaaac ccgccacgtt ctggaaacta
     3001 tccgcaaggt gatcgagacg gcgggtggca cgatggcgga tgtgaccttc aacagcatct
     3061 ttattaccga ctggaaaaat tacgccgcga ttaacgaaat ctacgccgag ttttttccgg
     3121 gtgataaacc ggcgcgattc tacattcagt gcggactggt aaaacctgac gcgctggtgg
     3181 aaatcgccac aattgcgcat atcgccaagt gaggccgcga tgaaactttc actctcacct
     3241 cccccttatg ctgatgcacc cgtagtggtg ttgatttcgg gtcttggcgg tagcggcagt
     3301 tactggttac cgcaactggc ggtgctggag caggagtatc aggtagtctg ttacgaccag
     3361 cgcggcaccg gcaataatcc cgacacactg gcagaagatt acagtatcgc ccagatggca
     3421 gcggaactgc atcaggcgct ggtggggatg cagctggcgc tggattatcc cgcgtcggta
     3481 actatgctgg tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt
     3541 caggttcgcg aacgattact gtatagcggc ggcgcgcaag catgggtgga agcgcagccg
     3601 ttgttcctct atcccgccga ctggatggcg gcccgcgcac ctcgcctgga ggcagaagac
     3661 gcgctggcac tggcgcattt tcagggcaaa aataatttac tacgtcgact taacgccctc
     3721 aaacgcgctg actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc
     3781 gccagtgatg atctgctggt gccaacagca tgttccagtg aacttcatgc cgccctgccc
     3841 gatagccaga aaatggtgat gccctatggc ggacacgcct gcaacgtgac cgatcccgaa
     3901 acgtttaatg ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc
     3961 ctgtaaggaa ttgctatgaa cgaagccgtt agcccaggtg cgcttagcac cctgttcacc
     4021 gatgcccgca ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacaggag
     4081 ctttatgccc tgatgaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg
     4141 tttatccgca ctgcagaagg aaaagaacgt ctgcgcccgg cgctttccag cggcaatctg
     4201 caaaaaaccc tgaccgcgcc cgtcaccgct atcgtcgcct gggacagtga attttatgaa
     4261 cggttaccac tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa
     4321 cttgccgaag aaacagcgtt tcgcaacagt tccatgcagg cggcttatct gatcgtcgct
     4381 tgccgtgcgc tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac
     4441 gacgcctttt ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc
     4501 gatagcagca aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg
     4561 ttgtaaggag caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg
     4621 tatgggcgcg gcggtcaata tcatcaccac ggacggtcca gccgggcgcg ccgggttcac
     4681 cgccagcgcc gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg
     4741 tggggcgtcc gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag
     4801 cgccgggcag gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt
     4861 tgccgccgcc cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt
     4921 ttcgtttgac tgccgtatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg
     4981 cgccatcgaa gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag
     5041 ttatcacgcg ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg
     5101 ttcggttttc ctcattggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg
     5161 gatgaacgat tgccgtttac tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg
     5221 tttggcgcga cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta
     5281 atgtcgggga tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat
     5341 ctcggctcca gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag
     5401 ggcattaacc cgaatatcag catcgcgctg ggcgggatta tcgcctgtgg gctggtttat
     5461 accgtcattg gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg
     5521 ccggtggtga cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg
     5581 aaaagtgtat cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt
     5641 gggctggtgg cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggatta
     5701 attgtcgcct gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc
     5761 gattttactc tcgtcagcta tgctgcctgg ttcggtctgc cgcatttttc cactcctggt
     5821 tttaatggcc aggcgatgat gttgattgcg cccgtggcgg tgattctggt ggcggaaaac
     5881 ttaggtcatc tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg
     5941 cgagcattcg tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc
     6001 gtcaccacct atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg
     6061 gtgtttgttg cagctgccgt catcgctatg ctgttgggct tttcgccgaa atttggcgcg
     6121 ctgatccata ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg
     6181 attgccgtcg caggcgcaag gatatgggta caaaaccgtg tcgatttaag ccagaacgct
     6241 aatttgatta tggtcgcagt aaccttcgtg ctgggcgcgg gtgattttgc tctcacgctt
     6301 ggcggtttta cgttgggagg gatcggtaca gcaacctttg gcgcgatttt actcaatgcg
     6361 ttattaagca gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa
     6421 acagaaaacc gccgggttac cggcggtgat gactttttga cgagcttaag aaaaaaatgc
     6481 attcgtcatt gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt
     6541 tttacctgct tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg
     6601 gtttgccatt actgacctcc gtctgggtat agacatcata ttgcatagaa ttaacgcggc
     6661 gtatggcggc tgccatattt tcgctacgtg ggactaagct tagtgaactg acgcggacag
     6721 acccgcgttt ggtaatatcc tgcaacagct tcttagcgag aatagatatc atctccaata
     6781 attatccata agccgctctt atgattaaaa aaacgcgatg agataaaaat gtgtcatttt
     6841 gcgacaaaat tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg
     6901 cactctgaaa aggacgtctt atctttaaat aagtggtagc gaatcgctac ggaatagaga
     6961 taacacgagg agtggttaga aatggctaaa gttctggtgc tttattattc catgtacgga
     7021 catattgaaa cgatggcacg cgcagtcgct gagggtgcaa gcaaagtgga tggcgcagaa
     7081 gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt
     7141 aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg ccgattacga cgccattatt
     7201 tttggtacac ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag
     7261 acgggcggcc tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt ctttagttcc
     7321 accggtactg gcggcggtca ggaacaaacc atcacatcca cctggacgac ccttgcgcat
     7381 cacggcatgg tgattgtccc cattggctac gcagcgcagg aattatttga cgtttcacag
     7441 gttcgcggcg gtacgccgta cggcgcaacc accatcgcag gcggtgacgg ctcacgccag
     7501 ccaagccagg aagaactgtc tattgctcgt tatcaagggg aatatgtcgc aggtctggca
     7561 gttaaactta acggctaatc ttcaacagga ggatacgcat gccaactcaa gaagcgaaag
     7621 ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc gccggaaata gccgaggcca
     7681 ttttcgaagt cgccgggtac gacgaaaaaa tggcagaaaa gatttgggaa gaaggtagtg
     7741 atgaagtgtt agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt tggggcgaac
     7801 agaccatcga acgtaaaaac gtttaaccag cgactccccc gcttctcgcg ggggagtttt
     7861 ctgttatttc accgcctcat tcaacacgct atcaaactta tccatcgggc agaaaccgtt
     7921 ggcatctatc gggcaaccgc ttaattccag cgtcacgcgc tgcgcaggtg cctgcagggt
     7981 taacgcatcg gcattacgta actgttccgc actctggtac acatattcaa ttttcatcaa
     8041 atcgcgattg gctttgctgt catgccaacg ctggaaaacg attttgccgc caatcggcgt
     8101 gcgttcgttc tggtcatgca actgatacgg tttgaaatcc agcgccgtta acagagaggc
     8161 tatgttggag tcgtgcccaa ctaacaccgt gattttcgga gcactggcgc gatcggtgac
     8221 cagggctttg tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga
     8281 ggtaaacaga ctgtcctggt aaccgttttt cagcttcgac aataccttcc actgctggtc
     8341 agatttgatt tctccccagg ccacctgatc catcggaaaa ccttcgtagt attgcaaagt
     8401 aaacgcatct accagcgagt tgccgacttt cagcggcccg gaaacacctg gttcttgttg
     8461 atacttggcg ctaaaggtat ttttgccatc caccagcgaa cactgttgtt tctctttaca
     8521 ggcaggggaa tctttatagt taacgatttt ttccagtagc tggtagctgt cggtaagctg
     8581 gagtttgctg agctctttct ccattgccgc caccgcctgt tcactgaatg cggcggaatc
     8641 atcggtgatc atcgggttaa aggttgggtc catagtgccc attttttcct ggtgatgcac
     8701 aggaatatca cacccaggga atgcaccagt aataaagaac tgggcggtag cgacggtacg
     8761 ttgcagacta ttggcatagg cataaacggt gtccggcggc gggcattccc ccgatttcac
     8821 tatcccctgc tctgccagcc attcacgcat gtaatggccc atatacactt cgagcacgcc
     8881 acctttggtg gtgagttgcc caccggggac gtcccattcg ggccatttat tcggcgtcga
     8941 ctgctccagc acactgccat tgttcgccag cggcgcacgt aagttatggc ggctcatcat
     9001 gagcacttgc tgtagctgat agccttccgg tacggtttgt gcctgagcat ttgaagcgag
     9061 taaaactatc cctgccacag ttgcggcgat tagcgttttg ttcattcctg gcacctcttt
     9121 tgttatcaat cattaaagag tgtgacagaa atatgacttt tttccgggaa caggttcgca
     9181 aaatggcttg ttgaacgcct gtttttctat atacatcttc aaaaacaggc aaggtttgac
     9241 gcaaactatg cctgattagc ccctgcctcg cgtacgccgc tgataattgc acctgtggct
     9301 aaacagaata tctgcggcgt taccaataca gagacaatat ttaccaataa aaaagtgtga
     9361 gactaataac aatggttttt agcatatgcc tttgccatta agttgcataa cgatttaaat
     9421 agtcaagttg cgcattcatt tccttatttt ttaacttata agcctgagaa aaaagttcag
     9481 gagggaggta aacatcataa tgattgccag tgtttgttaa ataaccatac tcaagatctt
     9541 catctgttga aaaggattca tttcccaagg agattgatgc cacattacta ttaacaataa
     9601 ataatgagca aatgctatct gaaagaacag caagaaaatt cttcaccgct ttaatcctat
     9661 ccgacgtgtc ggtatctaaa gcaagcaaat tcttgttatc attaaatata aactcgggta
     9721 aatgcagtat gcattttttg tcattcaagc aactatcaat gaattgtttt ttaaaaaaca
     9781 gaaagctcca tgacataaaa ctacccacac cctgaatttt gttgtcagat tgcagatcaa
     9841 cgccaaataa tgtctttaca tcttccctga gattatagaa tgccgtatca acaagagcct
     9901 cgatcgtttg tttatcaaca attttaataa actcttcgta tacttgcttt tgcgtcttat
     9961 ggctgcccag cgcccatgaa tactcatgtt cttttaaaac cagtatggct cttaacagac
    10021 aatatccatc gccatctact ggagcgtatt ttaatccata tgttttatta atttcactct
    10081 gaataaaaga tatgccagac aaatcaatac cagtatctaa atcagatatt cccggcatat
    10141 ttatctgttc tggctggata ctcatatttt catcatctaa acaatactca gaaaaaacat
    10201 catcacacgc attaatgttt tcaatatatt ccataaagcc ataccaataa taacctttaa
    10261 aatctgtgat agactctgaa cctctgcccg gcaaggacac tctaacaata tcaacatctt
    10321 gaacatattg tatagtatat gtaatacctc ctgaaacccc tgaataaatt tgtgtatcat
    10381 tagtacgcaa tgttctcata aataatgata gatcattatc attctgcatt tcctcaaatt
    10441 ctttttctag tgattcacat tccagacatg gagtatcctc ttcatttact ttacgtctga
    10501 gttcattgta caaccgcata aaacacacca gtgtgttttc aaaaagcgtg attgaatatg
    10561 tttgattttt ttcgtactca ttttttatat cgttccaggc ctgtttcaaa taattatttg
    10621 cagtgcaact aattccatgt atattactac ccatatatag cgtctataaa atttaataaa
    10681 taatgacgcc ctagttaaac ttaaagtgcc tggttcaact atcaaaaatc gctcaccctt
    10741 tttcacctgt ttaaaatatg ttcagcaacc catcttgatg gcgacctcct ctccgcgatg
    10801 atttcaataa catattctgt gttggcatat gaaattttga ggattaccct acacttatag
    10861 gagttacctt acaggggttc cttcaatttg tgttgattta cgcgagataa cgctatggaa
    10921 ttaaaggatt attacgccat catgggcgtg aaaccgacgg acgatctcaa gacaatcaag
    10981 accgcctatc gtcgactggc ccgtaaatac catcctgatg tcagcaaaga accggatgcc
    11041 gaagcccgct tcaaagaggt cgctgaagcc tgggaagtgt taagtgatga acaacgtcgc
    11101 gctgagtatg atcagatgtg gcaacatcgc aacgatccgc aatttaaccg tcagttccat
    11161 catggcgacg gtcagagttt taatgccgaa gattttgacg atatcttctc gtcaattttc
    11221 ggtcagcatg cccgccagag ccgtcaacgc cccgccgcac gcggccacga tattgaaatc
    11281 gaagtggcgg tattcctcga agaaacgctt actgagcata agcgtaccat cagctataac
    11341 ctgccggttt ataacgcctt tggcatgatc gaacaggaaa tcccgaaaac gctgaatgtg
    11401 aagatcccgg caggcgtcgg caatggtcaa cgcatccgcc tgaaaggcca ggggacgccg
    11461 ggcgaaaacg gcggtccaaa tggcgatttg tggctggtga ttcatattgc gccacatccg
    11521 ctgttcgata ttgtcggcca ggatctggaa attgtggtgc cggttagccc gtgggaagcg
    11581 gcgctgggta ctaaagtcac cgttccaaca ctgaaagaaa gcattttgct gactatcccg
    11641 ccaggcagcc aggccgggca acgattgcgc gttaaaggca aaggtctggt gagcaaaaaa
    11701 cagaccggcg atctgtatgc ggtactgaaa atcgtgatgc cgccgaaacc ggatgaaaac
    11761 actgccgcgc tgtggcagca actggcagac gcccagtcgt cttttgatcc acgtaaagat
    11821 tgggggaaag cataatggct aatgttacgg tgacttttac tattaccgaa ttttgcctgc
    11881 ataccggcat ctctgaagag gagttgaatg aaattgtcgg tttgggggtg gttgaaccgc
    11941 gtgagatcca ggaaacaacc tgggtatttg acgaccatgc cgccattgtg gtgcaacgcg
    12001 cggtacgcct gcgtcatgaa ctggctctgg actggccggg gatcgcggtg gcgctgacgt
    12061 taatggatga tattgcgcac ctgaagcagg aaaaccgcct gctacgccag cggctttccc
    12121 ggtttgtagc tcatccgtga tgtgtgtttg ccggatgtga tgacgcatcc ggcaactgcg
    12181 ccataatgcc tgatgcgacg ctaaacgcgt cttttcaggc gcataaacga cgttatctgt
    12241 tttggtggtc gcactccact aacaccagca ataactggct tagtgccgcg taaaaaccaa
    12301 agctgtcata ctgtcgacaa cgcgcagcaa actctggtaa ccattgccgc agcgccgtca
    12361 gtgttttttg ccgcaaactg tcgattcttc gcgcaggaac ggtcccctct cccagcgaaa
    12421 aatgcagata actcaacaac tcgagataga tcgccagatg atctgccggt tcgttgaaat
    12481 tgccactggt ttccatccct gcctcaacta acaagcgttt aatctcttgc tcgtcctgtt
    12541 tgtaggccga tgcatacggc agcgccgctt gtttgtcggt catcagaaac aggccgcaaa
    12601 agtcagcggc cagttccaga cgggcat
//
LOCUS       DAJTRB010000109        12155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTRB010000109 DAJTRB010000000
VERSION     DAJTRB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.109"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            128..997
                     /locus_tag="OO330_003434"
     CDS             128..997
                     /locus_tag="OO330_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5884184.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="OO330_003435"
     CDS             complement(1200..1553)
                     /locus_tag="OO330_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5884185.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="OO330_003436"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="OO330_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5884186.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="OO330_003437"
     CDS             complement(3381..3674)
                     /locus_tag="OO330_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5884187.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="OO330_003438"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="OO330_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5884188.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="OO330_003439"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="OO330_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5884189.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="OO330_003440"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="OO330_003440"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5884190.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /gene="dcuA"
                     /locus_tag="OO330_003441"
     CDS             7589..8890
                     /gene="dcuA"
                     /locus_tag="OO330_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5884191.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="OO330_003442"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="OO330_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5884192.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /gene="dsbD"
                     /locus_tag="OO330_003443"
     CDS             9320..11017
                     /gene="dsbD"
                     /locus_tag="OO330_003443"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5884193.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="OO330_003444"
     CDS             11054..11629
                     /locus_tag="OO330_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5884194.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="OO330_003445"
     tRNA            11736..11811
                     /locus_tag="OO330_003445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2930 a         3140 c         2981 g         3104 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgactg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcatccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttagagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaagcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtac agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg acgctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcctt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc catggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgtcagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atctaatatc
     7561 aacttgttaa aaaacaagga aggctaatat gctagttgta gagctcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgag cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcaaacgcat ctcaacttta cacaaatcaa
    10681 aacggtagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgctgccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggacct ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attcttcccc acatcaccag caatttgcga
    11941 gcatatccca atttgggaaa atttgttcct gagctatagc actaactgac gtgaaatgtc
    12001 gtgcggcccc gtgatgctgt tgaacgtcaa atgacgtcat caggagcgta acgtatccat
    12061 aaagcacaac accggtctga acgccgactg atgagatttt ctgaatgaga acaaagagaa
    12121 atgtgtcagt ccgcttgctc atgcaaagac taaca
//
LOCUS       DAJTRB010000110        12124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTRB010000110 DAJTRB010000000
VERSION     DAJTRB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.110"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..395)
                     /locus_tag="OO330_003446"
                     /pseudo
     CDS             complement(1..395)
                     /locus_tag="OO330_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            519..1259
                     /locus_tag="OO330_003447"
     CDS             519..1259
                     /locus_tag="OO330_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HCR5884195.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(1380..3359)
                     /locus_tag="OO330_003448"
     CDS             complement(1380..3359)
                     /locus_tag="OO330_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCR5884196.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAEYHELYCAGHLIEAGVAFFQATGRRRLLE
                     VVCRLADHIDSVFGPNEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SYASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHRHPEVG"
     gene            complement(3370..4770)
                     /locus_tag="OO330_003449"
     CDS             complement(3370..4770)
                     /locus_tag="OO330_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5884197.1"
                     /translation="MTSTPITTADIANGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGHGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4996..5811
                     /locus_tag="OO330_003450"
     CDS             4996..5811
                     /locus_tag="OO330_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5884198.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFQKHTGLTPAAWKRRYSKEHINS"
     gene            complement(5843..6538)
                     /gene="araD"
                     /locus_tag="OO330_003451"
     CDS             complement(5843..6538)
                     /gene="araD"
                     /locus_tag="OO330_003451"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5884199.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETYEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6532..7392)
                     /locus_tag="OO330_003452"
     CDS             complement(6532..7392)
                     /locus_tag="OO330_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR5884200.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAAAQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKALHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(7385..8047)
                     /gene="ulaD"
                     /locus_tag="OO330_003453"
     CDS             complement(7385..8047)
                     /gene="ulaD"
                     /locus_tag="OO330_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5884201.1"
                     /translation="MSRPLLQLAPDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     SAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHTMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(8044..9540)
                     /gene="lyxK"
                     /locus_tag="OO330_003454"
     CDS             complement(8044..9540)
                     /gene="lyxK"
                     /locus_tag="OO330_003454"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HCR5884202.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVFTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKVG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTLAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(9544..10530)
                     /gene="yiaO"
                     /locus_tag="OO330_003455"
     CDS             complement(9544..10530)
                     /gene="yiaO"
                     /locus_tag="OO330_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HCR5884203.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEAITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(10543..11820)
                     /gene="yiaN"
                     /locus_tag="OO330_003456"
     CDS             complement(10543..11820)
                     /gene="yiaN"
                     /locus_tag="OO330_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR5884204.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(11823..>12124)
                     /locus_tag="OO330_003457"
     CDS             complement(11823..>12124)
                     /locus_tag="OO330_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5884205.1"
                     /translation="DNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
BASE COUNT         2886 a         3200 c         3248 g         2790 t
ORIGIN      
        1 attgcaggtt tttagcgata aggtgatatc accgatatct ttaattttat cgattacatg
       61 tttggataaa taggtaatta tgccggttgg ggcgtagatg gtgacccgac cacgtttctc
      121 atgcttataa tctgcaataa aattattaag ttgctcgttt ctgtccagca tgtcgttgat
      181 gtacggtaac agcgcggtgc caaaaggtgt gagcatcagc tgccgggtgg ttcggtcaaa
      241 gacttttaaa cccacttttg attcaaaatc agcaagatat ttgctgacgt tggcctgtgc
      301 gatgccaggt acagtccggc atggctgata ttttcattgg cagcgattac cgagataatt
      361 tttaactccc ggtacttaag ttgtaatttt gtcatcgcaa cagcccaatt atatataatt
      421 ttatatatta ctatataaat atgaatcttg caccggagaa attgtaagca ttattatgcc
      481 gactcttttt tatcttattt taaaatggaa taccggacat gttaattaat cgcaatattg
      541 tggcgttatt tgcgttgcct tttatggcaa gcgcaactgc ttctgaatta tccattggtg
      601 ctggtgcagc ttataatgaa tcgccttatc gcggttataa tgaaaataca aaggcaattc
      661 cgctgattag ttatgaaggt gattcttttt atgttcgtca gaccacatta ggttttattc
      721 tgtcgcaaag tgaaaaaaat gaacttagcc tgaccgcatc ctggatgccg ctggaatttg
      781 accctgccga taatgacgat tatgccatgc aacagcttga taagcgtgat agtacggcta
      841 tggcgggggt tgcctggtat caccacgagc gttggggaac cgtgaaagcc tctgcggctg
      901 cggacgttct ggataacagc aacggctggg tgggggagct atcggtattc cacaaaatgc
      961 agataggtcg cctgtcgctg acacctgcgc tgggcgttct ctattatgac gagaatttca
     1021 gtgactatta ctatggcatt tcagagagtg agtcccgtcg tagcggtctg gcaagttatt
     1081 ccgcgcagga tgcatgggtg ccctatgtca gcctgacggc aaaatacccg ataggagagc
     1141 acgtcgtatt gatggcgagc gccggataca gcgagctgcc ggaagagatt accgacagcc
     1201 cgatgattga tcgtaatgag agttttacct ttgtcaccgg cgtgagctgg cgtttttaat
     1261 tcaccggtgg atgtcggtgc ggctggccta cgctctcgca ccgactcgta tgcctgataa
     1321 gacgtgttaa gcgtcgcatc aggcaatgtg ctccctacgt tggatgcggc gcgagcgcct
     1381 tatccaacct ccggatggcg atgcttttcc tcattcaccc agatccgcat ttcgccttcg
     1441 ccccggttag cccagctaaa ccacgggata aaggtcagag tttgcggctg gcgtttggct
     1501 ggcgcgctgt cgtaatgcca cagcggttgc tgctctggat tgctctgttc ataccggtaa
     1561 cccggtgcct ggattaagat cttatggctg aacaatccct tgccttcaaa tgtcgtaaat
     1621 ggcgcatcgg tgggcagcca cagattatgc agtgactcgc cgttgtcggc ctgttctagg
     1681 caatacacca gcggaccgcg ctgaatcgcc acttttccgg cgacgtgacg caccagcggg
     1741 ttaccgtaaa cgcggcgtac cggcatcggc aaagtcagat tcagcgtatc gccctcctgc
     1801 cattcgcggg taatgtgcaa atacccttta cgaatatcct gctcgacctc ttccccattc
     1861 aatatgatct gcggctgtgt gcaccagtcc ggcagacgta aagccagcgt atgacgtacc
     1921 ggctggggcg attcaaccgc aatcgtcacc tgctcctgcc acggatagtt cccgctaacc
     1981 cgcaggcgca gcgtgccatt ttctaccggc acttccatgc tgtttcctgc gtatatgttg
     2041 atatacaacg catcttcacg cggcgtgtag agataatgac caatcgaggt cagcacgcgg
     2101 gcgatatttg gcggacaaca agcgcagcca aaccaacgct ggcggatcgg tttaacgtga
     2161 tcgtagatat ggttgaattt cagcgatttt ggatgtactt ccagcggatt cacatagaag
     2221 aaatgtttgc catccagcgc catgccgccg agcacggtgt tgtacagcgc gcgctccatc
     2281 acatcggcat attgactgtc gccttccatt tccagcattc gccgggcgaa catcatcagg
     2341 ccgatggaag cataactttc ggcgtaaacc gtgtcattcg gcagatcgta atcgctagtg
     2401 aacgcttcgc cgctgctttg cgagccaatg ccgccggtaa tatataactg acgctgggcc
     2461 atattgttcc acagcctcag gcagtcctga cgcttgctgt catcgtgact taaacgcgcg
     2521 agatgcgcga cgccggtcat caggtagaca aaacgtaccg cgtgaccgat ggcggtttgc
     2581 tgttgcgcaa ggggcaaatg tgcctggctg taggctttgt ctttcaccat ccacgccggg
     2641 ccgtaggtgt gccagtgcga ggtctgcccg cgcttttcat attcctggtc gtaatagtgc
     2701 ggttgcgcac cacgctgttc gacaaaatag ttcgtcagcg ccaggtagcg cggctcttcg
     2761 gtgacttcgt agagacgcat cagcgccagt tcaatttccg gatgaccagg ataaccctgc
     2821 agcttgtctt catttggacc aaatacgctg tcgatatgat cggccagacg gcaaaccact
     2881 tccagcaagc gccgcctacc cgtggcctgg aagaaggcga ctccggcttc aatcagatga
     2941 ccggcgcagt aaagttcatg atactccgcc agattgctcc agcgttcttc gggtgctttt
     3001 accgtaaagt aagtattgag atagccgtct tcacattggg cggaggcgat cagttcgatt
     3061 acctcgtcgg cggttttttc cagttcggcg tccggcttct ggcacagcga ccaggctacc
     3121 gcttccagcc atttggcgac gtcgctgtcc tgaaacacca tcccgtaaaa ttcaccctcc
     3181 tgaagtccgg cagcaatgcg aaagttttca atcgcatggc tgggttccgc ttctgggata
     3241 cgatcgttca aggcatccca ctgataagga atcaccacgt cgcggaccag ttgttggtac
     3301 tgaccgagga acggatcgct gaccgtcagt ttacgcagat cgacttccga aatgttcatc
     3361 ttgtgctcct taaggctgta catgacgctg acgcaggtcg gtagctatgc gtgacatcat
     3421 aggattattg agtttgcaaa agcggatcgc ccctgccagc agcaggtgga atagcgcagg
     3481 cagtaatgtt tccatcgccg cgatcccgcc aagcgaactg gcggtctggt tttccacgcc
     3541 aggctggtag gcgacaaaaa taaacagcag gctgatgatc ccggcgctgg aggcccaggc
     3601 cagcttgatg aaaaacagat tgaaggcgaa gttcatgccg gaagaacgta cgcgggtttt
     3661 ccactcgccg tagtcatcgg caaaggccat caatgagaag tgcaatggca atgtgaagcc
     3721 aagaatcacg ccattaccga ggatcaccgc cagccacaga gtttgataag ccgggccgga
     3781 gggcaggaac cacatcaaca ccgccagtgc agcgaggagc aggttggtgt agtaataaat
     3841 tttgacggta tcgaaacgcc gcgttaacgg gctgacaatc accgagccga taatggaggc
     3901 gaaggtgacc atggtgaaga acagcgacgt gtagcccgta ctgccttgta agacgtaggt
     3961 aatgaagtac atatacccac cgccgcgaat gttaaagacg ttaatcagca ggaaagacat
     4021 gaccagcatc agcagcagtt ggtcgttgtt ccgcagcccg gcaagatgtt cgcgcagggt
     4081 aaatttcccc attgtggaga gcggcacccg ttcacgaacc cagaagaaac agcacagaaa
     4141 catcaccacg gcgatggcgc acagcacgcc gacgcccaat tgataaccgc gcgcggcatt
     4201 accatgaccg aagagatcta ccatccacgg taagcctacc gaaaccagaa atcccgctac
     4261 gccacagaga acgaatcgcc aggactggca ggagatcact tcattgtggc gggtggtcat
     4321 ggtgttgatc agcgcgcagt acggtacgtt gatggcggta taaccgactg aaaggagcag
     4381 gtaagtaccg aacgcccagg cgatttttac tcccatgctg gcatctggca cggtgaacgt
     4441 caggatgccg ataatgccga tgggaatggc aatccatagc tgccacggac gaaagcgccc
     4501 ccaacgactt tgcgtccggt cggcaatgac gcccatcacc gggtcggaga tggcatcaaa
     4561 cacgcgcagt gcaataaaca gcgtgccaac cagcgccgga gttaatccaa aaacgtcggt
     4621 gtagaaaaag gtgagaaaat tcatgatcag gcaagttatt acggtgccgc ctgcgtcacc
     4681 caggccgtag cctatttttt cccgtacaga taacgcatcg tcaattttac cgttagcgat
     4741 atcagcggta gtaatcggtg tagaggtcat agatattcct cgtgaatagg gagcaatttt
     4801 tagttttatt gaaatgacct gattaattct gccgtgaacg ggaatatcaa caaggcgaga
     4861 aaagagtaaa aaaaggtgac gattccgacc tgaatttaaa agtgtgaaat ggatcaggtg
     4921 aaattaatgt ttttgattaa taatcaaaag gtaatgtatt taatcattaa tgaataagcg
     4981 ggaaattaca tatcaatgct cgaattatcc atgacgttac cgattaaagt gcaaaatggt
     5041 ggattattta tttcccgcgg cgtagggagg catcctgcaa ggcggctgga gtcatgggaa
     5101 attatctttg tcgaaaaagg tcgattaacg attcaggaag aagaacggat atttctggtc
     5161 gatgcgggag agagtttatt actctggccg aatcgacaac atgttggggt ggaggatttt
     5221 cccgccgatc tcaaatttta ctggctgcat tttgaggtta aagcgccgga tagcgacccg
     5281 cgctggctca ctcatcttag cgtgccgcaa caaacgaagg ttgccgatcc gcaggcgctg
     5341 gtggccctgt ttcgccagtt cttaagcgaa caggaaaagc atcaacgcac tccggcgctg
     5401 gagtttattg tgctgctgat tttgcagcag ctcacccgtg acgcgcgtct tgatgaacag
     5461 gccgatggcg caggcaccac ccttgcctgg aaagcgcaga aactgatccg cacgcaatat
     5521 catcgcccgc tttctacggc gcttctggcc cgcgagctgt actgcaatgc cgattatctt
     5581 gggcgcgtct accggcgggt gtttcatctg acgttaacag aagcaatcca tcgccagcga
     5641 gtgcgcgaag ccgaaaagct gttaattagc gaagcgcgat cgctcactga agtggctgcg
     5701 ttgtgcgggt ttaatgacgt tggctatttc cggcagattt ttcagaaaca tactggttta
     5761 acgccagcag cgtggaagcg gcgttacagc aaggaacata tcaattcgta gtgccggggc
     5821 gatgaagccc ctgcgtgagg gattactgcc cgtaataggc attggcccca tgcttacgca
     5881 ggtagtgctt atccagcagt tcgttttgca tcgcagggag ctgcggcgca agctggcgcg
     5941 agaataggcc catataggcg cattcttcga gtactacggc gttatgcacg gcatcggcgg
     6001 cgtttttacc ccatgcgaac gggccgtgag aatgcaccag caccgccggg atttgtgccg
     6061 gactcctgcc acgttcttcg taggtttcaa tgatcacttc gccggtctga tattcatatt
     6121 cgccgttaat ttcctctgcg gtcatctgtc gcgtgcaggg gatggcacca tagaaataat
     6181 cagcgtgcgt ggtgccccag gcggggagat ccagcccggc ctgcgaccag atggtggcgt
     6241 ggcgcgagtg ggtatgcaca atgccaccga tttcggcgta gcgacggtaa agcgccagat
     6301 gcgttggcgt gtcggacgag ggctttttgc taccttccac caccttgccg ctggctatct
     6361 caaccaccac catatcgtcg gcggtcatca cgtcatactc gacgccggaa ggtttgatta
     6421 ccatccattg ccgcgtttcg tctaccgcgc tgacattgcc ccaggtgaac gtcaccagat
     6481 ggtgagcggg aagcgccaga ttcgccgcca gcacgtcggc tttcagttgc tctaacatat
     6541 aaatccagcc tcctgcatac gcgcttcaat ccaacgccgc gcctgaataa tctccagcac
     6601 cggctctttg gctttttcgg tccacatctc aatcaggaaa gaacctcgat agttcagttc
     6661 atgcagcgct ttgaagatgc caacgaaatc gacgcagcct tcgccaaacg gcacgtcgcg
     6721 gaactgtccg ggactttgct cggtaactgg ctgggtgtct ttcaggtgga tcgcggcgat
     6781 acggtcaatg cccagtttca gttcggcggg aacatcattg ccccaggcgc tgaggttgcc
     6841 gacgtccggg tagacggtga accacggcga ggcgagcatc tcgtcccact ttttccattt
     6901 actgatggag ttcataaacg cggtgtccat aatctccacc gccagcatca cctgcgcggc
     6961 tgctgcctgt tcaaccgtcc acgccagccc ttcagcaaaa cgttgccggg tgccttcatc
     7021 gtggtcttcg taatagacgt cgtagcccgc cagttggatg gtgcgtatgc cgagatcgcg
     7081 tgccagacga atcgctttgc tcatgatctc ccgtgctcgc tggcgtaccg cgtcgtcacg
     7141 gctaccaaaa ggaaagcgac gatgtgcgga caggcacatc gacgggatcc caacacccgt
     7201 ttcgatcatc gcggcaacca gcgaagtcct ttgtgcggtg ctccagtcaa gacgtgaaag
     7261 ccgttcgtcg gtttcatcca ccgacatttc gacaaaatca aaaccgcagc tttttgccag
     7321 caccagccgc tccggccagg agagatcttt cgccagcgcc ttttcataaa tccctaacgg
     7381 atgattacgc acgcttgcct ccccagatag cgtcgatttg cgcatggaaa tcgccagcca
     7441 cctgcgccgg atttgctgag cctgccagtg ctcgcccggc aataaacgct ttcacgcgaa
     7501 tatctttaaa cagcggcagg tcagcagggg taatcccgcc ggtaatggaa agctcaaggc
     7561 cgatatcgga aagcgccttc atgcgtgcca gatcggcttc gccccactgt tgcccgctgg
     7621 cctgtgcatc acgaccgcga tgataaatcg cctgccgcac gccaatacga tgccagtcgc
     7681 gggcgtcgtc cagcgtccag ttaccgaaca actcgatctg aatttcacca ccgcagcgtt
     7741 gcgccatcgt gtggcctttt tccaccgtcg cgagcggcgc ggcgcagatg atggtcatcc
     7801 agttagcgcc tgcgccaaac gcctgttgcg cgagcgtttc accagcgtcg gcgaccttcc
     7861 agtcggcaac gatgattttg tccgggcatt gttcgcgcaa ggctttcacc gcgctaagcc
     7921 cttcgtttaa acagagaatg gtgcccgctt cgacgatatc gacgctgtct tttaacagcg
     7981 tcacgtcgcg ctgcgcggct tcaagtgatg agtggtcggg tgccagttgc agaagtggtc
     8041 ggctcataat gtgtgctcct tgatgcgggc gtgaaagccc tgaagtgcgg caatgagatg
     8101 ctgatagcgt tggtattttt gttggtaaag ctgatgcgcg gtcatatccg gcagcagggt
     8161 gcgtaccggg tgctgtaagt cacgttgagc ttcgctgaaa tcgcgataaa ctccggtgcc
     8221 gacgcgggcg gcaagtgccg cgccaaaaca gccggtttct tccacctgcg gcagctcgat
     8281 acgcagaccg ctgacgtccg ccagcatttg catccagaca tcggagtgcg ccgggccgcc
     8341 agtgacgcgc agggtgtgca catcagtaaa acgttcgcgc atccggttga ggtgggtcat
     8401 atggctaaac accacccctt catagatggc ctgcaacagg tgcgcgaggg tgtgaatggc
     8461 ctgcatcccg tagaaaccgc tggtcatctc gagtccggcg ttgctgccgt acaggaacgg
     8521 caggaaaaag agatcgccac cgaccttcgg caagctggca acggcctgat tgatctcatc
     8581 aaacgagatt tctccccact gcgcggtaaa ccattcgagg ttgccggaag aggtagggct
     8641 ggcttcgtga acgataaatt gaccatcgtt aacgtagcga ccatagacat acggatgcgc
     8701 ttcaccgtca cgtaaaccgc gggttatgcc gctggtcacc gcccaggtgc ccatcactgc
     8761 gttgagggtg aattcgtctt cgatcccggc gcagagtgcg gtgaaaacca catcaaacag
     8821 gccgccgact acgggcgtac ccgctttcag accggtcagt gcggctgtct gagcggtgat
     8881 ttccccgcag atttcggcag aaccgacaac aggcggcagg gcgtgattga tttcagcgat
     8941 ccccagccag tcggtgaggc acgggtcata ttccccaaga ctcatgttgt agaggttgga
     9001 ctcggaaata ttgctctctt cacagccttt gacgccggtt aaacaccagc gcaggtagtc
     9061 gtgcgtcatc atcacgcagc caatttgcgc gtagcgttct ggttcgtgct ctttaagcca
     9121 acgtagcagc gacaccggat gcccggtcca caaggtttgt cgggtcagcg ggtagagttt
     9181 ttccgggatg ccatcttctt gccagcgacg aacgatttcc atcgcccggc gatccgagga
     9241 caaaatggcg ttaccgagcg gcttgttgtt tttatccagc aaaaacaagc cttttccttg
     9301 tgcggagata ccgataccga caatttgttc cccgctaacg ccggaatggg taagcagttc
     9361 gcgaatgaca gccatgcagc attgccacag ttctgccata tcgcgctctg cccaacctgg
     9421 ctgcgggctt aatgcgcaca gcggcaggcg ctgcacgcct gcctcccggc cttcgcggtc
     9481 atacagcccg gctttcagcc agctaccgcc acaatctaac cccagccagt attgcgtcat
     9541 ggtttattgc acctcatcca cggctttcag tagatcagca ccctgcggaa catcttttac
     9601 aaacatgtcg cggacctgag tacccagtgc gtcgctaaag gctttgcgat cgagatcggt
     9661 gatggcttcc acgcccgctt ctttcatgcc gtcgatgatt ttttgctgat cttcggcaac
     9721 cagtttgcgc tggtagttac ccgcttcctg cgcagatgaa attagcgctt gctggaactc
     9781 cgggcttaag ccatcaaact tcgctttgtt gatcaccacc agaagcgggg aataggcgtg
     9841 gtgcgtcaga gaaaggtact tctgcacttc gaaaaatttt gctgaccaga cgacgttgat
     9901 cgggtgttcc tgagcgtcga tagtgcgggt ttccagcccg gtatagactt cggcaaacgg
     9961 catcgggatc gggttagcgc caaagacttt gaatgcggct atattcatcg ggctattgtt
    10021 ggtgcgaatt ttcagccctt tcaggtcggc gggggtttta accggtgcgc gcgagttggt
    10081 gacatcgcgc cagccgtttt cccagtaggc cagtactttt agtcctttac cttcaagtga
    10141 ggctttcaga tcatcaccga ctttgccgtc gagcgtttta tgcgcgtgag cggtatcgcg
    10201 gaacaggaaa gggacatcaa gcaagttcat cactggtgat aacccggcaa agttattcga
    10261 gccggacatt tccatatcga tggtgccgcc acgtacgccg ctgatcatcg cctgcgcgtt
    10321 accgagggtg ctatccggaa acaattttaa tttcagctcg cctttggttc tctcctgcaa
    10381 taaatcattg aattttttcg ccgcaatatg ttgcgagtcg gtttgtgagg tttcataacc
    10441 gaaacgtaaa gattgcgccg ccagagaaga tgtgctgaat gcagccaggc cagcaatgaa
    10501 taatgcgtag gttacagagc gtaatttcat aatattttcc ttttaattaa tccatttcaa
    10561 agggaggatg atgagatcgg gaataaaaac aaatacgact aataatgaat ataaaaccag
    10621 gacgtaaggg aaaacgcctc tgactgcatc atcgaatttg agttttgcca cccctgaaat
    10681 aacgttaagt acattaccga taggcggtgt aattaagccg attgagcagt taatgataaa
    10741 catgacaccg aaataaatcg gatcgattcc tgcctcttta actaaaggca ttaataccgg
    10801 ggtaagaatt aataccgtcg gcgttaaatc catgaccatg ccgacaatca ggatagccac
    10861 cataatgacg ataaacagca gacgcggtga atcgaccaac ggttgcagta aatcagaaac
    10921 catcatcggc agttcagcaa tggtaatcag ccaggcggaa acttgtgccg aggccaccag
    10981 aaacataacc actgaggtgg ttttcgccgc gccaatgagt acatgccaaa gtgtggcgaa
    11041 ggtcatttca cggtaaataa ctgtggcgac aaacagcgca tagaaagcag caacggcccc
    11101 ggcttcggta ggggtaaaca gaccggagcg aaacccgcca ataatgatga ctggaagaaa
    11161 cagcgcccag ataccggaga caaaagagtg ccagatttcc tgcatcgttg ctttttgctg
    11221 gcggggtaaa ttaaggcgac tggcctgccg ccaccaggta agcatcagcg ttgcgcccat
    11281 cattatgcca ggggcaatgc ccgccataaa cagcttactg atggataatc cgctggaaac
    11341 accgaagata ataaacggaa tggaaggggg aataattggc gcgataatgc cgccagaggc
    11401 aatcagcccc gccgcccggt tgaccgggta gttggcgctg cgcatcatcg gcaccagtag
    11461 agcggcaacg gcggcggtat ccgcaacggc agaaccagaa aggctcgcca taatcatagc
    11521 tgccagcacg ccgacgtagc ccagcccgcc tggtttatgc cccaccagtt tcatcggcag
    11581 gtcaacaatg cgttttaaca agccgcccgc attcatgatt tcacccgcca gcacaaagaa
    11641 cggaatcgcc aggagggaga agctatcggc tccgttcacc agcgtttgtg ccatgatctg
    11701 gacatcaaac atgtccagcc agaacattaa cgccgccccg cacaacaaca gtgcccaggc
    11761 aataggcaaa ccgatagcaa taccacccaa cagacagccc agaaaaatca gcacagccat
    11821 gattaagctc cttgcggtgg cgacgttaga gaattgctac gcgtgatgag ttgatataaa
    11881 tgacgcagtt caaagaatgc gataacgagg ctggtgggca gacaagcggc atacatcagg
    11941 ccgatgggca aaccgaggat cggtgaataa tcgctccagt cctgaattgt ttttagcgtt
    12001 gcgccccagg ccagtgcgcc acaaataaat aagattaagg aatgggtaac cagagcgact
    12061 cgtcgttgcc atgcggggga gagtttctcc accagaaagg tgacctgaac gtgggcgtta
    12121 tcca
//
LOCUS       DAJTRB010000111        12112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTRB010000111 DAJTRB010000000
VERSION     DAJTRB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.111"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..369
                     /locus_tag="OO330_003458"
     CDS             <1..369
                     /locus_tag="OO330_003458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884206.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO330_003459"
     CDS             complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO330_003459"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5884207.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO330_003460"
     CDS             complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO330_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5884208.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO330_003461"
     CDS             complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO330_003461"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5884209.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO330_003462"
     CDS             complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO330_003462"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5884210.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTVFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO330_003463"
     CDS             complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO330_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5884211.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5094..6755)
                     /gene="tar"
                     /locus_tag="OO330_003464"
     CDS             complement(5094..6755)
                     /gene="tar"
                     /locus_tag="OO330_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5884212.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVVWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="OO330_003465"
     CDS             complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="OO330_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5884213.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="OO330_003466"
                     /pseudo
     CDS             complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="OO330_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
     gene            complement(9393..10319)
                     /gene="motB"
                     /locus_tag="OO330_003467"
     CDS             complement(9393..10319)
                     /gene="motB"
                     /locus_tag="OO330_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5884214.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10316..11203)
                     /gene="motA"
                     /locus_tag="OO330_003468"
     CDS             complement(10316..11203)
                     /gene="motA"
                     /locus_tag="OO330_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5884215.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="OO330_003469"
     CDS             complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="OO330_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5884216.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11911..>12112)
                     /locus_tag="OO330_003470"
     CDS             complement(11911..>12112)
                     /locus_tag="OO330_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5884217.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2782 a         3386 c         2934 g         3010 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac
      361 tacaaataac catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg
      421 gcatcatcgc gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa
      481 atcgtccacc tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc
      541 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
      601 cagcaccatc agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac
      661 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
      721 aaagctggta tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg
      781 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
      841 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
      901 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
      961 ggcttccgcc gcttcggcaa tggcctgatc cagccccagt tcccgcaaac tgtcgcgcag
     1021 catatgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     1081 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     1141 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     1201 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     1261 atgccgacat cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg
     1321 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     1381 cgacaccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     1441 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     1501 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg
     1561 ctggcttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     1621 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     1681 acgcatcgcc aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac
     1741 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg
     1801 acggttaacc gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag
     1861 ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaagacac gttctccgtc
     1921 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     1981 gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg
     2041 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     2101 agaactcaac aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc
     2161 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat
     2221 tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc
     2281 gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg
     2341 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     2401 cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg
     2461 cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     2521 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     2581 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca
     2641 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     2701 tcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     2761 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     2821 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     2881 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg
     2941 gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg
     3001 gtgcccaatg tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc
     3061 gccgcgctcc atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg
     3121 aaatgatgag cctcacggaa aaataccgtc agattcgtgg tcagcgaatt gataaacgcc
     3181 tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc
     3241 agtcccagcg aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
     3301 gccagaacga tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
     3361 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
     3421 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
     3481 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
     3541 tggtcggcct gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag
     3601 ttctgctgag tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc
     3661 cgttgttctt ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa
     3721 ctgacaatat cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca
     3781 cgattgactg actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg
     3841 gcaagattgc gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga
     3901 gccgcttcca ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata
     3961 acgctgataa tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc
     4021 atggtactga cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc
     4081 gacgcctgtc gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg
     4141 gcggccgttt gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg
     4201 cctgcgacaa tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc
     4261 accgtcccac gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca
     4321 ttacgaccat ataccgcaat cggacgcgcc agattaccgg cagcaatgct gtcaaaatgg
     4381 ctaccgataa tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg
     4441 atgtagatcg ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta
     4501 cgctggcttt gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca
     4561 aagttaacgt caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt
     4621 tgattacttt caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt
     4681 ttctccgtct ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa
     4741 cttttaaaca gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta
     4801 atgtcatccg ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg
     4861 gcctgtaaca ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg
     4921 ccgtgattca gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca
     4981 atctgcaaga tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga
     5041 ttaaacatca acgctcccca tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat
     5101 gtttcccagt ttggatcttg ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca
     5161 ctggcaggac gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac
     5221 gcggaaaccg cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc ggcggcagct
     5281 gattcctgca ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat
     5341 gcgacttgat cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg
     5401 tcagtcacgc gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc
     5461 agcaccgaac cggtatcaat gcgcgagacg gagtcttcaa tgagggcttt gatctctttt
     5521 gccgcctggg cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca
     5581 cggccctgtt cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc
     5641 tggaaggcaa taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg
     5701 atctcatgca tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg
     5761 gaggcacttt gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc
     5821 gcggtgagct gctccatgct ggcggcagtt tcttccagcg cggatgcctg ctgttcagta
     5881 cgggaggaaa gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct
     5941 gaaccttcgc ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc
     6001 tgcgccaggt cacccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca
     6061 ccggcgattt cgcaaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga
     6121 atgccgtacc acaccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc
     6181 cactgggcaa atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca
     6241 ctgctgaggg cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc
     6301 tgagcgaaat aagctccagt attgccataa tcaagataat caatcagttc agttaacgct
     6361 gtgtaatagt ttttatattt ttcatcaaaa ttacggctgg tagcgaccat ttcaggtaac
     6421 ggtgccatgc ttttgaattt tttataatgc gtcgcggcct gggccaatgt tttcctggcg
     6481 ctatcgagca attcaacttt ggcgttactt tgttgattgg aggaatccat catcatccgc
     6541 accgctgaac gactcaggtt aatgcgcgtt tgcagcatta aatcccaggt tgacgtcagc
     6601 tcgccctgct gttcccgtaa ttgattggaa accacaaagc tcttctggct atggtgaagg
     6661 gaagaaaaaa acaaactgcc ggaaataagc tgtaacagtg cgaatacccc cagcaccatt
     6721 accaacagcg tgactacgcg gatacggtta atcataaggc accttcctga aaacaagttg
     6781 atctcgttat cggcaagaag gggggaaact ttattgctga tgccacccgc cgcgaaattg
     6841 aaataaaaaa cccgatgcgc agatcatcgg gttcatttca attgaggaaa tcgggagaat
     6901 tacgccactt ctgacgccgc gctatctaac agcgccatct cttcgctgtt cagcagtttt
     6961 tcgatgttca ccagaatcaa catacggtcg cccagtgcgc ccagtccagt gagatattct
     7021 gttgaaagcg tcacggcaaa ctctggtgcc ggacgaattt gctccgccgt caatgaaagc
     7081 acgtctgaaa cgccgtcaac cacgatgccg accacccgct gtccgagatt caggacgata
     7141 actaccgtgt tgtcgttata gtccacatcc acctggctga acttaattcg taagtcaaca
     7201 atcggcacaa taacgccgcg cagattcgtg acgcctttga taaacgctgg cgtgttcgca
     7261 atccgtgtta cctgatcgta gccacggatc tcctgcactt tcaggatatc aataccgtac
     7321 tcttcatcac caagggtaaa taccagaaat tcctggcctg acggctcgct ggccagcttt
     7381 gttacattcg tcataccggt catattgtta cctttttact cattcaggcg gcggtgttcg
     7441 ccatacgttg ttcgcggttt atcgcctgca aggcggagac atcaacaatc agtgccacgc
     7501 tgccgtcgcc aagaatggtc gcagcagaaa tgccggggac tttgcgatag ttactttcaa
     7561 ggtttttaac cacaacctgg tgttgaccaa ttaattgatc caccagcaag gcgtagcggc
     7621 gaccgccact ttgtaagatc accacaattc cctgggtggc ttcggttttc gcgcccgcga
     7681 cgttgaacac tttccacagt tcgacgatgg gcagatattc accccgcact tccagcaccc
     7741 gctcgccgcc ggccagtgga tggagatcgg cttcacgggg ttgcagtgat tccataacag
     7801 cattcagcgg cagaatgaaa acttcatccg caacgcgtac ggacatgccg tcgaggatgg
     7861 ccagcgtcag cggcagtaaa atgcggatcg tagtgccagt accctgcttc gactggattt
     7921 cgacatgacc gcccatctcc tggatattac gtttaacgac gtccatgccg acgccgcgcc
     7981 cggagacgtc ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc agcattgcaa
     8041 cttcgtcgtc gctcatgttt tcgctgacag tcaaaccttg cgaggccgct tttgccagaa
     8101 ttcgctcacg gtttagcccc gccccatcgt cggtcacttc aatgcaaatg ttgccgccct
     8161 gatgttcggc agacagaatt aaatttccga cgctgttttt acctgcggcg agccgttttt
     8221 ctggcagttc aataccgtga tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa
     8281 tgcgttctat caggctcttg tcgagctcgg tggaactgcc caccagcgtc agttctacct
     8341 gcttgccgag ttttcccgcc agatcacgca ccagccgggg atagcgacta aagacatatt
     8401 ccatcggcat catgcgaatc gacatcactg attcctgcaa atcacgggcg ttacgttgta
     8461 actgccccat gctggtaatc aaatcaccat gattaaccgg gtccagttcg ctggaacgct
     8521 gggcaagcat ggactgggtg ataaccagct cgccgacgag gttaattaat tgatcaacct
     8581 tttctaccgc tacacggatg ctggtggatt cactgctgcg ggtcgttttt tcccgctcca
     8641 cgcggccggt tggcgcttgt tcggctgcca gtttaagcac aggtggggta gctatttttg
     8701 gcgaaacttc tactgtttca aaggtaatct gatcggcttc aatcacaaaa cagagtaccg
     8761 ctgtgatgtc atcttcggcg atatcgcccg gtaatattgc cgagagagaa tccgcccctt
     8821 tcaccacgtc atttaacgtt gtcagatgcc ccagctcttc ttccagcagg tcgacttcgc
     8881 cggccttcag gcgcgaaagg ataattcgtc gcggcaactg actgcgactt tgctcatctt
     8941 gcggttcact tttggcaacc acacttaatc gggtcactgc ggatggcgtt tcgcctttcg
     9001 cttctaatgc cagttgacgc aaggcctagc agatataatc gtagctggcg gcatccggct
     9061 cttgcgactg tttataagcg tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca
     9121 gattgataat gtcggtgttg agttgcatct cacctcgtct ggcttcatcg agcaggtttt
     9181 ccatcagatg cgtggtttcc tgcaaaacgc tgaagccaaa agttcctgcc cctcctttga
     9241 tcgagtgggc agcccgaaag atggcattca attgttcggc atctggcgct tccggctgca
     9301 aaaccagcag atgctgctcc atatcagcca acagttcgtc cgcttcatca aaaaatgtct
     9361 gataaaaatc gcttatatcg atgctcacgc tgtcacctcg gttcggctga tggcattgtg
     9421 ggaacactga cctgtggtgc aacctcaggt ttttctaggg cgcttactgg ctcattctgg
     9481 ctttcggcgt tttcatgcaa aatggcctgt tcggcttgtt tgttcagtac caacaggctg
     9541 atgcgacggt tgacggcatc atcaggtccg cgatcgctta agcgcatcgt tgccgccatg
     9601 ccgacgacac gtaacacttt gccgctatcc aaccctccga ccatcagttc gcggcgggat
     9661 gcattggccc gatcggcaga aagctcccag ttgctgtaac ctttctcacc gctggcgtag
     9721 ggaaaatcat cggtatgacc tgaaaggcta atacggttgg gaataccgtt cagcacaggc
     9781 gcgatggcgc gcagaatgtc gcgcatatag ggttcgacat cggcactgcc ggttctaaac
     9841 atcgggcgat tctggctatc gatgatctga atacgtagac cttcctggac cagatcgatt
     9901 ttgagatgga gacgtaacgc ccgcagtttc ggatcggact ctatcaactg gtcgagatca
     9961 ccccgcaatt tccgcaatcg actttgctcc atgcgttttt tcagctcttc gatgttcggc
    10021 tgcttattca cttccccctg gctttgggtg taatcatcac caccgccggg aattgggctt
    10081 tcactattgg aaatgcgatc gccgcccgta accgctgtcg ccagtggagt ccggaagtac
    10141 tccgcaatct gaatcaactc ttttgggctg gagatggaga tcagccacat caccagaaaa
    10201 aaggccatca tcgcagtcat aaagtcggca taagcaatct tccacgatcc gtgtgctgcc
    10261 ccgtggcttt tggctttgcg tcgtttgacg acaataatcg gatgcgcttg attcttcatg
    10321 cttcctcggt tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc tcttccagtt
    10381 caataaacga cggacgttcg ctggaataga gcgttttgcg accaaactca acggcgatag
    10441 gcggtgcgta accgttcaga ttagaaagca gagtgacttt gacgcactgc atcattttgc
    10501 tggtttcggc gcttttctga cgtaaaacac tcgctagtgg ggaaataaat ccgtaagcca
    10561 ataaaatacc gaggaaagtc cccaccatcg catgtgcgat aagcgccccc agctcggcgg
    10621 caggacgatc ggctgaacct aacgcgtgaa cgccccccat tacagccgcg acaataccaa
    10681 acgccggaag tgagtccccg accagcgcca gactgttcgc cgggacttct gcctcgcttt
    10741 cgtgcgtctc aatctcttca tccatcaggg cttcgatttc gaaggtgttc atgtgaccgc
    10801 tgataatcag gcgcagataa tcgacgataa aatcaagcat gacgctatcc gcgaggatgc
    10861 gtgggtagct ggcgaagatc tcgctctcac ggggattttc aatatcacgt tccagcgaaa
    10921 acatccccat ctgccgcgat ttcgccatca accgataaag cagagccagc agatccatat
    10981 acattgcttt ggtgtatttg gagcgacgaa acagcaacgg cagcgctttc agcgtgcctt
    11041 taatcgcttt gccattattg ccgacgataa acgacccaat ccctgcaccg gcaataatca
    11101 ccagttcagc gggttgatag agtgctccaa ggcttccacc ggtcatcaaa taaccgccga
    11161 aaactgtacc gagaacaacc aggtaaccta ataagataag cacgacatca tccttccact
    11221 gttgaccatg acaggatgtt cagtcgtcag gcgttaacgc gcgattgggg aaaaaaaaag
    11281 cagcggtacg tcgttaccgc tgctggaatg ttgcgccaca ccgtatcagt taaacagcct
    11341 gtactctctg ttcatccagc agttgtggga taatatcggc aggattctgg gaaagtttac
    11401 gtctttttac tgcccgggat ggcggttgac ataagctgca ggcaaagctg ccaacaggct
    11461 ggtgagcgtg ggtaataaaa ttgccgccgc agcagttgca gctggaaagt tgcagtaatc
    11521 cactttcaac aaaccgcacc aatgtccagg cacgggttaa tgccagcagt ggtccttctt
    11581 ctgcttgtgg gcactgttca aggtataaac ggtaggcttt gatcaccgca tcgacgccat
    11641 tacacaaacc ggttttcagt aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt
    11701 tttgttccca ggtcataaac cagtcggttg agaatggcag catgcctttc ggcggtgggc
    11761 ttccgcgcag ttctttataa agttttatca ggcgtccgcg acttaactgt gtttcgcttt
    11821 ccagcatctg caaacgagcg cccagggtga tcaattccat tgccagctga atatcccgcg
    11881 cttcctgaac aatgcttttt tcactcatga tcaggccctt ttcttgcgca gcgcttcttc
    11941 aggctgatta acatcattca gcaagcgtgt tgagagcatg atgccggtat gaatttgctg
    12001 gagatcgtca acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg
    12061 gaagtgacaa accagctgat tggtttctgc cagcttaacc atttgcggaa ga
//
LOCUS       DAJTRB010000112        12008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTRB010000112 DAJTRB010000000
VERSION     DAJTRB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.112"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(18..394)
                     /gene="rnpB"
                     /locus_tag="OO330_003471"
     ncRNA           complement(18..394)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO330_003471"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(427..1572)
                     /gene="garK"
                     /locus_tag="OO330_003472"
     CDS             complement(427..1572)
                     /gene="garK"
                     /locus_tag="OO330_003472"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5884218.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1669..2559)
                     /gene="garR"
                     /locus_tag="OO330_003473"
     CDS             complement(1669..2559)
                     /gene="garR"
                     /locus_tag="OO330_003473"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5884219.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2589..3359)
                     /gene="garL"
                     /locus_tag="OO330_003474"
                     /pseudo
     CDS             complement(2589..3359)
                     /gene="garL"
                     /locus_tag="OO330_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3375..4709)
                     /gene="garP"
                     /locus_tag="OO330_003475"
     CDS             complement(3375..4709)
                     /gene="garP"
                     /locus_tag="OO330_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5884220.1"
                     /translation="MILDTVDAKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5084..6589
                     /locus_tag="OO330_003476"
     CDS             5084..6589
                     /locus_tag="OO330_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HCR5884221.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVGLNHLYGCGVA
                     INAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQPERLLTGTDDVQAIPVEIASI
                     VSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRETCPASELVVGMHCGGSDAFSGVT
                     ANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYL
                     NMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSPGQRPTKRGLIYAA
                     TPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINA
                     GTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT"
     gene            complement(6641..7465)
                     /gene="agaR"
                     /locus_tag="OO330_003477"
     CDS             complement(6641..7465)
                     /gene="agaR"
                     /locus_tag="OO330_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5884222.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7714..8994
                     /gene="kbaZ"
                     /locus_tag="OO330_003478"
     CDS             7714..8994
                     /gene="kbaZ"
                     /locus_tag="OO330_003478"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5884223.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9017..9490
                     /gene="agaV"
                     /locus_tag="OO330_003479"
     CDS             9017..9490
                     /gene="agaV"
                     /locus_tag="OO330_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5884224.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9501..10280
                     /locus_tag="OO330_003480"
     CDS             9501..10280
                     /locus_tag="OO330_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5884225.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10270..11079
                     /locus_tag="OO330_003481"
     CDS             10270..11079
                     /locus_tag="OO330_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5884226.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11166..11600
                     /locus_tag="OO330_003482"
     CDS             11166..11600
                     /locus_tag="OO330_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5884227.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDVLGRCHEECPVEEGI"
     gene            11597..>12008
                     /locus_tag="OO330_003483"
     CDS             11597..>12008
                     /locus_tag="OO330_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5884228.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAH"
BASE COUNT         2856 a         3236 c         3066 g         2850 t
ORIGIN      
        1 gggtttttac gtaaaacagg tgaaactgac cgataagccg ggttctgtcg tggacagtca
       61 ttcatctagg ccagcaatcg ctcactggct caagcagcct acccgggttc agtacgggcc
      121 gtaccttatg aacccctatt tggccttgct ccgggtggag tttaccgtgc cacggactgt
      181 taccagccgc gcggtgcgct cttaccgcac cctttcaccc ttacctgatc ccgcttgcac
      241 gggccatcgg cggtttgctc tctgttgcac tggtcgtggg tttccccccc aggcgttacc
      301 tggcaccctg ccctatggag cccggacttt cctcccctcc gcccgtctcc cccgaagagg
      361 acgacgacga agcggcgact gtctggtcag cttcggcgcg cagtatagag ggtttgcgcg
      421 cccttgtcac cccgtgttgc gcattccaat cgccagtgtc gcggcaatat tacgtgaagc
      481 acggcagata ttgtcataag ccccgcggaa tgcttcgtcc aacgtaccta tgctggtcaa
      541 tacgctgaag accgcatcaa tgccatgctg atgtacaacg ccaacatcat cggtcaggct
      601 acccgcaatg ccaatcaccg gtttatggta cttcttcgcc acgtttgcga cgccaatcgg
      661 taccttgccg tgaatactct ggctgtcaat acgcccttca ccggtgatca ccagcgtaca
      721 atcgtgaata tgttcctcca gattcagcgc cgtagtgacg atttcaatac cacttttcag
      781 ttccgcacca agaaacgcca tcagcgccgc acccataccg cccgcagctc ctgcgccggg
      841 gacatctttt acatcaacat gcagcgcttt tttaatgaca tcggcatagt gagagaggtt
      901 attgtccagc tcaacaatca tcgcttcact ggctcccttt tgtgggccaa agatgcgcga
      961 tgcgccgtta tcgcccacca gcggattggt gacatcacaa gcgacgcgaa tgacgcaatc
     1021 ttttaagcgc ggatcgaggc cggaaatatc aatatcattc agagtattaa gactaccgcc
     1081 gccaaaacca atttcattgc cgttggcgtc gcataatttc gcccccagcg cctgtaccat
     1141 gcctgcgccg ccatcatttg tagcgctacc gccaatgccg ataataatgt ttgtcgcgcc
     1201 gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt gaagtggtca cgagtggatc
     1261 gcgtttttcc gcaggtacca gctccagccc actggccgcc gccatttcaa taaacgcggt
     1321 tttgccatcg ccggagatcc cccaactggc attcactttc tcgcccagcg gccctgtaac
     1381 ccaggcgtga cgttcagaac cctgggtggc tgcaatcatc gcttccaccg ttccttcgcc
     1441 accgtcggca accggaatag aaacgtactg tgcatcagga aaaatttccc gaaatccttt
     1501 ttctatcgcc tgcgcaacct cgctggcaga taaactttct ttataagagt ctggggcgat
     1561 tacgattttc atacctatgc ctgttaccac atgacgccgg agggcgtttc tcttattcgg
     1621 cctggattcc aggcccggat tgcaatacgc catccgggca cgacgtcatt aacgagtaac
     1681 ttcgactttc gccagttttt cgtagtagca cgccagggcg ctatgatccg ccgttcctaa
     1741 accatctgct cgcagtgcct gcatcatctc cataaccgca gctgtgagcg gcagttgcgc
     1801 gccgacgccg tgggaagtat ccagcgcatt tgccagatcc ttaatatgca gatcaatacg
     1861 gaagcccggc ttgaagttgc ggtccatcac catcggcgct ttggcatcca gcacggtact
     1921 gcccgccagt ccaccgcgaa ttgcctgata caccaggtcc gggttaacgc ccgctttagt
     1981 tgccagcgtt aacgcttctg acatcgcggc aatattcagc gccacaatga cctgattcgc
     2041 cagtttggtg acgttacctg caccaattgc cccggtatgc accacggaac ccgccattgc
     2101 tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg ccgcccacca tcactgacag
     2161 cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga gcatccagca tatcaatgcc
     2221 tttcgctttc agcgcttcgc tgatttcacg gcttgccagc ggtgcgatag aactcatatc
     2281 gatcaatacc gtacctggct tcgcgccttc aataatgcca ttctcaccca gcgccacctc
     2341 tttcacatga ggggagtttg gcagcatggt tatgatgacg tcgcactgtt cagcgatcgc
     2401 tttagccgta gaggctgttt ctgcacctgc agcaatcacg tcagcaatag cttctgggtt
     2461 acggtcagca accaccagcg agtaacctgc tttcagaagg tttttactca ttggtttacc
     2521 cataatcccc aggccaataa aaccaacttt catagtcata tcaatcatct ctcttgttgc
     2581 ggtggtggtt attttttaaa ggtatcagcc agtttatgag tggcagagcg gaagacgccg
     2641 agatcgctgc cgacagccac aaacgtcgcg ccccattcca gataacgacg cgcatcggct
     2701 tcgaccggcg cgaggatacc gctgggtttg ccgtgcgcgc tggcacggtt aaaaatgtgc
     2761 tgaattgctt tttgtacatc cgggtgtgat gcattgccga gatggcctaa tgccgcggcc
     2821 agatcgctgg ggccgacgaa gatgccgtct acgccttcgg tagcggcaat ggcatcgacg
     2881 ttatctacgc cctgctgact ttctatctgg accagaatag tgatgttctt gttcgactga
     2941 gcgaaataat ccgccacggt gccaaacata ttggcgcggt gagaaacgga gacgccgcga
     3001 atgccttccg gtgggtaacg ggttgatgcc accgcctgct ctgcttcctc ttttgtttct
     3061 acaaaaggaa tcaggaagtt atagaaaccg atatccagaa gacgcttaat aatttccggc
     3121 tcgttggtcg gcactcacac tactggcgcg ctggcgctgc ctttcaaggc cattaactgc
     3181 ggaataaacg tggagatatc gtttggcgca tgttcgccat ccagcaccag ccagtcaaac
     3241 ccagccaaac caagaacttc agtgctaatc gggttagaga gtgctgacca gcaaccaatt
     3301 tgtacctgtt tcgcagccag tgcggctttg aatttattcg ggaaaacatc gttattcatc
     3361 gcttatacct ttgcttattt ctgcaattcc atacgtttaa tgtcgccaac tacgaagagg
     3421 tagcagacca tcgccatcag cgctgaacat cccacgaaaa ccagtgctgc attgaaggag
     3481 tgcagttcac ttaccaggta gccaatcacc agtggagtga caatggaggc aacattgcca
     3541 aagacgttaa agacgccgcc acagaggcca acaatctctt tcggcgcggt gtcagaaatc
     3601 accggccagc ccagagcacc aaatcctttg ccaaaaaaag ccagcgccat cagcatgacc
     3661 accagcgtgg tgttgttggt gtagttacat aagatgatgg tggaagccag caacattccc
     3721 agcacaatcg gtagcttacg tgccagggtc agggataaac cgcgtttgat cagataatcc
     3781 gagaagacac ctcccagcac gccgcccgca aaaccacaca gtgccggaat cgaggcgacc
     3841 agacccactt tcagaatcga catgcctttt tcctgcacca gataaatcgg gaaccaggtg
     3901 aggaagaacc aggtgatggt gttgataaaa tattgtccga aaaatacgcc caacatcatg
     3961 cggttagaga gcaattgctt gatgtaatgc agtttgggac cgcttgctgc cgcgctgccc
     4021 ggctttttgt ggtccatatc gaccaccgcg ccattttcag agataaactt cagctcttcc
     4081 tcagacatac gtgggtgatc tgtcgggtta tgaatcaact tgatccacag cgccgtcagc
     4141 acaaaacaaa tcacccccat aacggtaaag acgtgctccc atccccaggc gaaagtcagc
     4201 cagccaagca gaggcgaaaa gagcgccagc gagaaatatt gtgccgagtt aaagatggcg
     4261 gaggcagtac cacgttcttt cgtcgggaac caggcggcga caatacgggc gttcgccggg
     4321 aatgatggag cttccgagaa tcccagcata aagcgcataa agaacatgga gatccctgcc
     4381 caggccagcg ggaacatatc aacaaagcct tgcaggaagg tgaacagcga ccagaaaaag
     4441 aggctgtagg tgtaaacttt tttcgagcca aacttatcaa gcaaccagcc gccggggatt
     4501 tgcatcagca agtaggccca gccaaaagcg gagaagatgt aacccatcga aaccgcactt
     4561 aactgcaact cttttgccac ttcggtacca gcaatagaca gcgttgcacg atcggcgtag
     4621 ttaacggcgg taacaataaa aataatcagt aatattaaat agcgggtatg cacacctttc
     4681 ctttttgcgt caacggtgtc cagaatcatt ttatttacct cgggtactta tgctgatttt
     4741 tattattatg gggaaggtgt tatttataag tttcatttat gccgtaacga caatgaactc
     4801 gggaattagt ataagcagcg cgagaataat aatcattgtg caaatgctaa tttaattaat
     4861 actttttaaa tattattttg agcatatgca cataaggttg cgcgctaaag cacagatttg
     4921 cgctttacct tgccgggcgg cactgcaatc cctgaaatga ttgacattga tcacatttct
     4981 gcgtttaaac tcctgacatt cttatttcac ccaatgaaat catttatttt taaatgagac
     5041 caggtcctca ttttaataac ccctggctgg agaatattgc acaatggcca acatcgaaat
     5101 cagacaagaa acgccaactg cgttttatat aaaagttcac gacacagata atgtggcaat
     5161 tattgttaat gataatggcc tgaaagcagg aacgcgtttt ccggatgggc tggaattaat
     5221 tgaacatatt ccccaggggc ataaagtcgc attgctggac attccggcta atggtgaaat
     5281 tattcgttat ggcgaagtga ttggttacgc cgtgcgtgca atcccacgcg gaagctggat
     5341 cgacgaatca atggttgtac taccggaagc gccgccgtta cacacgctgc cactggcaac
     5401 caaagtcccg gaacccttac cgccgctgga aggatacacc tttgagggct atcgcaatgc
     5461 cgatggcagc gtgggcacca aaaacctgct cggcatcacc accagcgtcc actgtgtggc
     5521 aggcgtggtg gggctgaatc atttgtacgg ttgtggcgtg gcgattaacg caccggcagc
     5581 agttgtacct attcgtacca ttcacaatat ttcgctgaac cctaactttg gcggcgaagt
     5641 aatggtgatt ggcctgggtt gtgaaaagtt gcagcctgag cgcctgctga ctggaacgga
     5701 tgatgtgcaa gctattccag tagaaatcgc cagcattgtc agtttgcagg atgaaaagca
     5761 tgtcggtttt cagtccatgg tcgaggatat tttgcaggtc gccgaacgcc atctacaaaa
     5821 actgaatcaa cgtcagcgag aaacctgtcc ggcttcagaa ctggttgtcg gcatgcattg
     5881 cggtggcagc gatgcgtttt ctggcgtaac ggcaaacccg gcggttggct atgcgtctga
     5941 tctactggtg cgctgcggcg caacggtgat gttttcagaa gtcacggaag tgcgtgacgc
     6001 gatccatctg ctgacaccac gcgcagtgaa cgaagaggtc ggcaaacggc tgctggagga
     6061 gatggagtgg tacgataact atctcaatat gggaaaaacc gaccgcagcg ccaacccttc
     6121 gccgggcaac aagaaaggcg gtctggcaaa cgtggtagag aaagcgctcg gctccattgc
     6181 taaatcgggc aaaagtgcaa ttgttgaagt gctgtcgccc ggtcaacgcc cgactaaacg
     6241 cggattaatt tacgccgcga cgccagccag cgattttgtc tgtggcacgc aacaggtggc
     6301 ttcgggtatc accgtgcaag tgtttacgac cggtcgtggt acgccgtacg gcctgatggc
     6361 ggtacccgtc attaaaatgg caacccgcac cgagctggcg aaccgctggt ttgatttaat
     6421 ggatattaat gcgggcacca tcgctaccgg cgaagaaact attgaagagg tgggctggaa
     6481 gttgttccac tttattctcg acgtcgccag cgggaagaag aaaaccttct cggatcaatg
     6541 gggactgcat aaccagctgg cggtgtttaa cccagcaccg gtgacctgat aattcaattc
     6601 gtattatgcc cgatagcaca cgtttatcgg gcatattgag tcacagcgaa gacgcgtgat
     6661 ccccgaccag aatcacttca accccagcct ttcgcagtcc ttccagacta tccgcaggaa
     6721 tgccttcatc aacaatgatc atgtcgatac gttgagtatc aatgatctta tgtaaactgg
     6781 aacgattgaa tttactggaa tcggtgacca cgatgatccg ttccgcaact tcgcacatcc
     6841 gacggtttaa acgggcttca tcttcattat gtgtgctgac gccgcgctcc agatcgatcg
     6901 catctacacc aagaaacagc atatcgaagt ggtaattttg cagcgattgc tcagcctgat
     6961 cgccgtaaaa agattgcgac tgacggcgca aatgcccgcc ggtcatcagc agctcaacgc
     7021 cttccgcttc cagcaacgca ttagccacgt tcataccgtt ggtcatcgca attacgtcag
     7081 tgtacttgcg catcagacga gcaatctcaa aagtggtggt cccggaatcg aggatcaccc
     7141 gatgacctgg ctgaatcaac tcaacggcag ctttcgcaac gctgcgtttc atcgcggtgt
     7201 tcagtgcgct tttatcttcc actgatggct cgactgacgg cgtcgtgcta tcgcagatca
     7261 acgcgccacc ataggcacgc acagcgatcc cctgcttttc cagaaacgcc agatcattgc
     7321 ggatcgtcac agtagatacg ccatacaatg ccgacagatc gttaacctgc acactccctt
     7381 gctgtcgcag acgctgaatg atctgttctc gtcgctcgct ggtgcctgtc actcgcttct
     7441 cacctgaagc gtcggtatta ctcatagtaa gtcctttcgt aaaactttcg tttcatttcg
     7501 ttttgcctat taacgccttt ctattaagca aatgcaagcc caccttgccc attggcgcaa
     7561 gctactctcg tttcagtgac tttcattacg tttcttttgt gaatcagatc agaaaactat
     7621 tatctttcgt tttattttta tctcaccatg acgcagtatc aattgaaaca aaacgaaaga
     7681 tcaatatcgc agtaatctga actggagagg aaagtgaaac atctgacaga aatggtgaga
     7741 cagcacaaag cgggcaaaac aaatgcaatt tatgccgttt gttccgcaca tccgctggtg
     7801 ctggaggctg caatccgcta cgccagtgca aaccaaacgc cgttactgat tgaagcaacc
     7861 tccaatcagg tagaccagtt cggcggttat accggaatga cgcccgccga ttttcgcggc
     7921 tttgtttgtc agctcgccga ctcgttgaat ttcccgcagg atgcgctgat tctgggtggt
     7981 gaccatctgg ggccaaaccg ctggcaaaac ctgccagccg ctcaggcaat ggccaatgcc
     8041 gatgatttga ttaaaagcta cgttgcggca ggattcaaaa aaatccacct tgattgcagc
     8101 atgtcctgtc aggacgatcc aattccctta actgatgaca tcgtggctga acgcgccgcc
     8161 cgtctggcga aagtggcgga agaaacctgt cgcgaacact ttggcgaagc cgatctggag
     8221 tatgtcattg gtaccgaagt gccggtacct ggcggcgcgc atgaaacctt aagcgagctg
     8281 gcggtcacca cgccggatgc cgcccgcgcc acgctggaag cccatcgtca cgcctttgaa
     8341 aagcaaggtt tgaatgccat ctggccacgc attattgccc tggtggttca acccggcgtc
     8401 gaattcgatc acaccaacgt tattgattat cagcccgcca aagcgagcgc cttaagccag
     8461 atggtcgaaa actacgaaac gctgattttc gaagcgcact ctaccgatta tcaaacgccg
     8521 caatctctgc gccagctggt gattgaccac tttgccattc tgaaagttgg cccggcgctg
     8581 accttcgccc tgcgtgaagc ccttttttct ctggcggcga ttgaagaaga actggtacca
     8641 gcgaaagcct gttccggtct gcgtcaggtg ctggaaaacg tgatgctcga ccgcccggaa
     8701 tactggcaaa gccactacca cggtgacggc aacgcgcgtc gtctggcgcg tggttacagc
     8761 tactcagatc gcgtgcgcta ttactggccg gacagccaga ttgatgacgc tttcgctcat
     8821 ctggttcgta atctggcgga ttcaccaatt ccgctaccgc tcatcagcca gtatctgccg
     8881 ctgcagtacg tgaaagttcg ctccggcgag ctgcaaccga cgccacggga actcattata
     8941 aaccatattc aggacatcct ggcgcagtac cacacagcct gtgaaggcca ataagcaaaa
     9001 caaagaggaa catgctatgc caaatattgt tttaagccgg attgatgaac gcttgattca
     9061 cggtcaggtc ggcgttcagt gggtcggatt tgcgggggca aatctggtgc tggtagccaa
     9121 cgatgaggtt gccgaagatc cggtacaaca aaacctgatg gaaatggtac tggcagaagg
     9181 gatcgccgta cgtttctgga cgctgcaaaa agttatcgac aacattcatc gcgccgccga
     9241 tcgacagaaa atcctgctgg tttgtaaaac acccgccgat ttcctgacgc tggtgaaagg
     9301 tggcgttccg gtgaatcgca ttaacgttgg caatatgcac tacgccaatg gcaaacaaca
     9361 aatcgccaaa acggtttctg tggatgcggg cgatatcgca gcatttaacg acctgaaagc
     9421 cgctggggtg gaatgcttcg ttcagggcgt cccgacagag cctgctgtgg acctctttaa
     9481 attactttga gggattcatc atggaaatca gcctgttgca ggcatttgcg ttgggcatta
     9541 tcgcctttat cgctggccta gatatgttta acggcttaac ccatatgcac cgcccggtgg
     9601 tcctcggccc gttggtcggg ctggtacttg gcgatctgca taccggaatt ttaaccggcg
     9661 atacgctgga actagtgtgg atggggctgg ccccgctggc gggcgcacag ccgcctaacg
     9721 tgattatcgg tactatcgtc ggcacggcgt ttgccattac tactggcgtg aaacccgatg
     9781 tcgcggtcgg tgtcgccgta cctttcgctg tcgcagtaca gatggggatt accttcctgt
     9841 tctcggtgat gtctggcgtg atgtctcgct gcgaccggat ggcggagaac gccgatactc
     9901 gcggcattga acgcgtgaac tatctggcac tgctggcact cggcactttc tattttctct
     9961 gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa aaccatcatt gatgtcctgc
    10021 cgcaacgatt aatcgacggc ctcggcgtcg cgggcggcat catgccagca atcggttttg
    10081 ccgtgctgct gaaaatcatg atgaaaaacg tctacatacc ctacttcatc ctgggctttg
    10141 ttgccgcaac ctggctcaag ttaccggtgc tggctattgc tgccgccgcg ctggcgatgg
    10201 cgctgatcga tctgctgcgt aaatctcctg aaccgactca acctgcggca cagaaagagg
    10261 aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg tcagcgaaaa tgaagaaaca
    10321 ctgctgactg gcgtcaatga aaacgtgtat gaagatcgga gcattgccgc ggagctgacg
    10381 aaaaaagata tcaaccgcgt cgcctggcgt tccatgctgt tacaagcgtc ttttaactac
    10441 gaacgtatgc aggcttctgg ctggttgtac ggtctgctgc ctgctctgaa aaagatccac
    10501 actaataaac gcgacctggc gcgcgccatg aaggggcata tgggtttctt caatacccat
    10561 ccgtttctgg tgacgtttgt tatcggcatt atccttgcga tggagcgttc taagcaggac
    10621 gttaacagta ttcagagcac caaaattgcc gtcggtgcgc cgcttggcgg gattggcgat
    10681 gcgatgttct ggctaacgct actgccgatt tgtggcggga taggcgcaag cctcgcgcta
    10741 caaggctcta ttcttggcgc agtcgtcttt attgtgctgt tcaacgtggt acacttgggg
    10801 ctgcgttttg gtctggcgca ttatgcttac cgcatgggcg tggcggcgat tccccttatt
    10861 aaagcgaata ccaaaaaagt cggccatgct gcgtcaatcg ttggaatgac ggtaatcggc
    10921 gcactggtgg caacctatgt tcgtttaagc accacgctgg aaatcacggc gggcgacgca
    10981 gtagttaagt tacaggctga tgttatcgac aaactgatgc ctgccttctt accgctggtc
    11041 tacaccctga ccatgttctg gctggtacgc cgcggctaga gtccgctgcg cctgattgcg
    11101 gttaccgtgg ttctagggat cgtcggtaaa ttctgccatt tcctttaaat acaatgaggt
    11161 attagatgtt aagtattatt ttgacagggc atggcgggtt tgccagtggc atggaaaaag
    11221 caatgaagca aatcctcggc gatcaatcgc agtttattgc catcgatttt cccgaaacct
    11281 caagcgccgc gctgcttaca tcgcagcttg aagaggccat cgcccaactg gattgtgaag
    11341 acggcattgt ttttctgacg gatttgctgg gcggcacgcc gtttcgcgta gcttcaacgc
    11401 tggcgatgca aaaaccgggc tgtgaagtga ttaccggcac caatttgcaa ctgctactgg
    11461 agatggtgct ggagcgcgaa gggttaagcg gtgaagaatt ccgtgtacag gcgctggagt
    11521 gtggacaccg agggctgacc agtctggtgg acgtgttagg ccgctgccat gaagaatgtc
    11581 cggtcgagga aggaatatga cacacgttct gcgcgccaga aggctgctga ctgaagaggg
    11641 atggttcgat gaccatcagt tgcgtgttgc tgacggtgtc atcgcagcaa tcgaaccgat
    11701 tccagtgggc gtgactgaac gcgatgcgga actgctctgc cccgcttaca tcgacaccca
    11761 tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg ccggatgtac tcgacaagct
    11821 ggcaatgcac aaggcacgcg aaggtgtcgg cagttggtta ccgaccaccg taaccgcgcc
    11881 gcttaatacc attcatgcgg cgctgaaacg tattgctcaa cgttgccaac gcggcggacc
    11941 tggtgcgcaa gtgctgggga gttatctcga aggaccgtac ttcacgccgc agaataaagg
    12001 cgcgcatc
//
LOCUS       DAJTRB010000113        11979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTRB010000113 DAJTRB010000000
VERSION     DAJTRB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.113"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..1084
                     /locus_tag="OO330_003484"
                     /pseudo
     CDS             1..1084
                     /locus_tag="OO330_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(1208..2010)
                     /gene="glcC"
                     /locus_tag="OO330_003485"
                     /pseudo
     CDS             complement(1208..2010)
                     /gene="glcC"
                     /locus_tag="OO330_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            2261..3760
                     /gene="glcD"
                     /locus_tag="OO330_003486"
                     /pseudo
     CDS             2261..3760
                     /gene="glcD"
                     /locus_tag="OO330_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3760..4812
                     /gene="glcE"
                     /locus_tag="OO330_003487"
     CDS             3760..4812
                     /gene="glcE"
                     /locus_tag="OO330_003487"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5884229.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPSTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4823..6042
                     /gene="glcF"
                     /locus_tag="OO330_003488"
                     /pseudo
     CDS             4823..6042
                     /gene="glcF"
                     /locus_tag="OO330_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6047..6451
                     /locus_tag="OO330_003489"
     CDS             6047..6451
                     /locus_tag="OO330_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5884230.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6473..8644
                     /gene="glcB"
                     /locus_tag="OO330_003490"
     CDS             6473..8644
                     /gene="glcB"
                     /locus_tag="OO330_003490"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5884231.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8999..10594
                     /locus_tag="OO330_003491"
     CDS             8999..10594
                     /locus_tag="OO330_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5884232.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2907 a         2883 c         3249 g         2940 t
ORIGIN      
        1 aggcatttat cgcttttccg tcatcacttt atcccgacga tccaaactgg ataccccctt
       61 tattcattga acgcaacgag catttgtctg cgaaaaatcc ggggacggac catatcatct
      121 ggcaggcgtg ggtggcaaaa aaagcggggc agatagttgg gcgtattacc gcgcaaatag
      181 ataccttgca ccgtgagcgt tacggtgaag ataccggtca tttcggcatg attgacgcca
      241 ttgatgatcc gcaggatttt gccgcgttgt ttggcgcagc ggaagcgtgg ttgaagtcac
      301 aaggtgcaag taagatcagc gggcctttca gcctgaatat caatcaggaa agcggattac
      361 tgattgaagg ttttgacaca ccaccctgtg cgatgatgcc acacggcaaa ccgtggtatg
      421 ccgcgcatat tgaacaactg ggttatcaca aaggcattga tttactggcg tggtggatgc
      481 agcgaaccga tctcactttc tctccggcgc taaaaaaact gatggatcag gtgcgcaaaa
      541 aggtgaccat tcgctgcatc aatcgtcagc ggtttgccga agagatgcag atcctgcgtg
      601 agattttcaa ctctggctgg cagcacaact ggggatttgt gccgtttacc gaacatgaat
      661 tcgcgaccat gggcgaccaa cttaaatatc tggtgccgga cgatatgatc tacatcgctg
      721 agattgattc tgcaccctgc gcgtttattg tggcttgcct aacatcaacg aggcgattgc
      781 cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc gtttgaaagt
      841 cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt atcagttcag
      901 ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacgcgatc cgtttgcccg
      961 ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag gcatgaataa
     1021 catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg aaaagcaaat
     1081 ttaataccag atcctgttta gcgcaggggc ggtcattcgg gagagggtat agatgaacag
     1141 atttaacacc agatcctgtt aagtgcaggg gcggtcccct cgccttgggg atagggtaaa
     1201 actctgctca cattcccacc ttgttcatct ccagcggcac cgccgagcga atcaaatggt
     1261 ggccttccag ctcgatatcg tggagattct ttttcacggt ccgtacatga tcgcgcgctg
     1321 cgcgctgcgc gctgcgcgct gagcgacgtg cggcagccgc tgcaacaccg cgttgtagat
     1381 ccgcgcatgc tggcgatcga tctgcttttt ttgctgtggt cgatggtaga gattatttac
     1441 gctggcaaac actgaattaa acatcagatc ggtcaatgat tgcagcgtaa acaccagcac
     1501 ctgattgtga gaagcctgac aaatggcgag atggaaagcg tgatccaact gcgcatgttc
     1561 gatcagcgaa atctctttgt ggttctcact ggcggcgagc attttttcat aacagcgggt
     1621 tatcacaaca aaatcagcct gcgttcccag cgttcccagc gttcccagcg ttgccgccag
     1681 ccttgccgat tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg
     1741 ctgcgtactg aacagatgga tcagcgggct ggtgtcctgc tcccgattaa gccgtgcgac
     1801 atgagaatcg cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc
     1861 ttcacgcagt gcggagcgtg agaagccgag cttttcacac agtcgacgtt ccgagggaag
     1921 cggctgaccg accttcagta cgccgtcgat aattaaccgt tcgatactct ctgcaaccac
     1981 ttcgcaaata gggcgacgtt catctttcat tcccggacct cgtgcacagg taggaccaat
     2041 ttttctgttc ttaactcgca aaacacgcac atcacgcaag tgattatgtt acatcaattt
     2101 aacattaagt taaccaagac aaggtcacag agctggaaaa aaaatggtct gaccggtagc
     2161 taaagagata gacgaaaacg aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa
     2221 aaatcagctg ccacacaaca caacaaagcg aagcctactc atgagcatct tgtacgaaga
     2281 gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga
     2341 gcatgtccct ggacttgaga tcctgcatac cgatgaggag atcattcctt acgagtgtga
     2401 cgggttgagc gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc aaatggaaca
     2461 ggtgacagcg attctggctg tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc
     2521 aggcaccggg ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc
     2581 gcgctttaaa gagagcctcg acattaaccc cgttagtcgc cgcgcgcgcg tgcagccggg
     2641 cgtatgtaac ctggcgatct cctaggccgt tgcgccgcat aatctctatt acgcgccgga
     2701 tccttcctca caaatcgcct gttcaattgg cggcaatgtg gcggaaaatg ccggtggcgt
     2761 ccactgcctg aaatatggtc tgaccgtaca taacctgctg aaaattgaag tgcaaacgct
     2821 ggacggcgag gcgctgacgc tgggatcgga cgcgctggat tcacctggtt ttgacctgct
     2881 ggcgctgttc accggatcgg aaggtatgct cggcgtgacc accgaagtga cggtaaaact
     2941 gctgccgaag ccgcccgtgg cgcgggttct gttagccagc tttgactcgg tagaaaaagc
     3001 cggacttgcg gttggtgaca tcatcgccaa tggcattatc cccggcgggc tggagatgat
     3061 ggataacctg tcgatccgcg cggcggaaga ttttattcat gccggttatc ccgtcgacgc
     3121 cgaagcgatt ttgttatgcg aactggacgg cgtggagtct gacgtacagg aagactgcga
     3181 gcgggttaac tacatcttgt tgaaagcggg cgtgactgac gtccgtttgg cacaggacga
     3241 agcagagcgc gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg taggacgtat
     3301 ctccccggat tactactgca tggatggcac catcccgcgt cgcgccctgc ctggcgtact
     3361 ggaaggcatt gcccgtttat cgcagcaata tgatttacgt gttgccaacg tctttcatgc
     3421 cggagatggc aacatgcacc cgttaatcct tttcgatgcc aacgaacctg gtgaatttgc
     3481 ccgcgcggaa gagctgggcg ggaagatcct cgaactctgc gttgaagttg gcggcagcat
     3541 cagtggggaa catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag
     3601 cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa
     3661 ccctgggaaa aacattccca cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca
     3721 tcacggtcat ttaccttttc ctgaactgga gcgcttttga tgctacgcga gtgtgattac
     3781 agtcaggcgc tgctggagca ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt
     3841 cagggcagca atagcaaagc ctttttaggt cgcccagtca ccgggcaaac gctggatgtg
     3901 cgttgtcatc gcggcattgt taattacgac ccgaccgagc tggtgataac cgctcgtgcc
     3961 ggaacgccgc tggtggcgat tgaagcggcg ctggagagcg ccgggcaaat gcttccctgc
     4021 gagccgccgc attatggtga agaagccacc tggggcggga tggtcgcctg cgggctggcg
     4081 gggccgcgtc gcccgtggag cggttcggtc cgcgattttg tcctcggcac gcgcatcatt
     4141 accggcactg gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac
     4201 gatctctcac ggttaatggc cggaagctat ggttgtcttg gcgtgctcac tgaaatctca
     4261 atgaaagtgt taccgcgacc gcgtgcctct ctgagcctgc gtcgggaaat cagcctgcaa
     4321 gaagccatga gtgaaatcgc cgagtggcaa ctccagccat tacccattag tggcttatgt
     4381 tacttcgaca atgcgttgtg gatccgcctt gagggcggcg aaggatcggt aaaagcagcg
     4441 cgtgaactgc tgggtggcga agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa
     4501 caactgccgt tcttctcgtt accaagtacc ttatggcgca tttcattacc cagcgatgcg
     4561 ccgatgatgg atttacccgg cgagcaactg atcgactggg gcggggcgtt acgctggctg
     4621 aaatcgacag ccgatgacaa tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg
     4681 acccgcttta gtgccggaga tggtggcttt gccccgctat cggctccttt attccgctat
     4741 caccagcagc ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac
     4801 gcggaacttt gaggagcagg ctatgcaaac ccgattaacc gaagagatgc ggcagaacgc
     4861 gcgctggaag ccgacagcat cctgtgcgcc tgtgttcact gcggattttg taccgcaacc
     4921 tgcccaacct atcagcttct gggcgatgaa ctggacgggc cgcgcgggcg catctatctg
     4981 attaaacagg tgctggaagg caacgaagtc acgcttaaaa cacaggagca tctcgatcgc
     5041 tgcctcactt gccgcaattg tgaaaccacc tgtccttctg gtgtgcgcta tcacaatttg
     5101 ctggatatcg ggcgtgatat tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg
     5161 ctgcgcgaag gattgcgcca ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag
     5221 gtagggctgg tgctgcgacc gtttttaccg gaacaggtca gagcaaaact gcctgctgaa
     5281 acggtgaaag ctaaaccgcg tccgccgctg cgccataagc gtcgggtttt aatgctggaa
     5341 ggctgcgccc agcctacgct ttcgcccaac accaacgcgg caactgcgcg agtgctggat
     5401 cgtctgggga tcagcgtcat gtcagctaac gaagcaggct gttgtggcgc ggtggactat
     5461 catcttaatg cgcaggagaa agggttggca cgggcgcgca ataatattga tgcctggtgg
     5521 cccgcgattg aagcaggtgc cgaggcaatt ttgcaaaccg ccagcggttg cggcgcgttc
     5581 gtcaaagagt atgggcagat gctgaaaaac gatgcgttat atgccgataa agcgcgtcag
     5641 gtcagtgaac tggcggtcga tttagtcgaa cttctgcgcg aggaaccgct ggaaaaactg
     5701 gcaattcgcg gcgataaaaa actggccttc cactgcccgt gtaccctaca acatgcgcaa
     5761 aagctgaacg gcgaagtgga aaaagtgtta cttcgtcttg gatttacctt aacggacgtt
     5821 cccgacagcc atctgtgctg cggttcagcg ggaacatatg cgttaacgca tcccgatctg
     5881 gcacgccagc tgcgggataa caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc
     5941 gtcaccgcca acattggttg ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt
     6001 cactggattg aaattgtaga acaagccctt gaaaaggaat aaaaaaatga aaactaaagt
     6061 cattcttagc cagcaaatgg cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa
     6121 aaataactgg tctgtttcca ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag
     6181 tcgcatggac gattgcgcgc cgattgcggc ttacatctcc caggagaaag cgcgtaccgc
     6241 cgcgctgggg cgtcgtgaaa ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc
     6301 gttcgtgact gcgccgttac tgacgtcgct ggaaggcggc gtaccggttg ttgttgatgg
     6361 gcaaattatt ggtgccgtgg gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc
     6421 gaaagtggca gcagcggtgt tggcgaaata agcgaaaacg aggagagaaa caatgagtca
     6481 aaccataacc cagggccgtt tacgcattga cgccaatttt aaacgttttg tggatggaga
     6541 agttttgccg ggcgtggaac tggacgctgc tgcattctgg cacaatgttg atgagatcgt
     6601 tcacgatctg gcaccagaaa atcgtcagtt gctggcagaa cgcgatcgca ttcaggcggc
     6661 gcttgatgag tggcatcgca gcaatccggg gccggtaaaa gataaagcgg cctataaatc
     6721 tttccttcgt gaactgggct acctggtgcc gcaaccggag cgcgtgacgg tggaaaccac
     6781 gggcattgac agcgaaatca ccagtcaggc ggggccacag ctggtggttc cggcaatgaa
     6841 cgcccgctac gcgctgaacg cggcgaacgc tcgctggggc tcactgtacg atgcgttata
     6901 cggcagcgac atcatcccgc aggaaggggc gatggtcagt ggctacgatc cgcagcgcgg
     6961 tgagcaggtt atcgcctggg ttcgacgttt cctcgatgaa tcactaccgc tggaaaacgg
     7021 cagctatcag gatgtggtgg cgtttaaggt ggtcgataaa caattacgca tccagttgaa
     7081 aaatggtaaa gaaaccacgt tacgtactcc agcacagttt gtcggttacc gtggcgatgc
     7141 cgctgcgccg acctgcattt tgctgaaaaa taacggcctg catattgaac tgcaaattga
     7201 tgccaacggg cggattggca aagacgatcc ggcgcacatc aacgatgtta tcgtcgaagc
     7261 tgctatcagt accattctcg actgcgaaga ttcggtcgcg gcggttgatg cggaagataa
     7321 aatcctgctg taccgcaatc tgctgggcct gatgcagggg actctgcaag agaaaatgga
     7381 gaaaaacggt cggcaaatcg tacgtaaact gaatgacgat cgtcagtaca ccgccgccga
     7441 tggctctgaa atttctctgc acggacgctc gttgctgttt atccgcaacg tgggtcattt
     7501 gatgaccatt cctgtgattt gggacagcga aggcaatgaa atcccggaag gtattcttga
     7561 tggcgtcatg actggcgcga ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac
     7621 tggcagcgtc tatattgtga aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa
     7681 caaactgttt acccgcattg agacaatgct cggtatggca ccgaataccc tgaaaatggg
     7741 cattatggat gaagaacgcc ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg
     7801 caaccgcgtg gcgttcatca ataccggttt cctcgaccgt accggcgatg aaatgcattc
     7861 ggtgatggaa gctggcccga tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa
     7921 agcctacgaa cgtaataacg tgctttccgg tctgttctgt gggctgcgcg gtaaagcgca
     7981 aatcggtaaa ggcatgtggg caatgccgga cctgatggca gacatgtaca gccagaaggg
     8041 cgaccaactg cgtgccgggg caaacacagc ctgggttccg tcaccaaccg ctgctacgct
     8101 ccatgcgctg cactaccacc ataccaacgt acagagcgtg caagccaaca ttgcccagac
     8161 cgagttcagt gctgaatttg aaccgctgct ggacgatctg ctgactattc cggtggctga
     8221 aaacgctaac tggtcggcgc aagagatcca acaagagctg gataacaacg tgcaggggat
     8281 tctggggtac gtggtgcgtt gggtggagca ggggattggt tgttcaaaag tgccggatat
     8341 tcacaacgtg gcgctgatgg aagaccgcgc aacgctgcgt atctccagcc agcatatcgc
     8401 caactggtta cgtcacggta ttctgaccaa agaacaggtg caggcgtcgc tggagaatat
     8461 ggcgaaagtg gttgatcagc aaaatgctgg cgatccggct tatcgtccga tggcggggaa
     8521 tttcgctaac tcgtgtgctt ttaaagctgc cagcgattta atcttcctcg gcgtgaaaca
     8581 gccaaacggt tataccgaac cgttattaca cgcctggcgt ttacgcgaaa aagaaagtca
     8641 ttaatagcgt caaaaaagcc ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg
     8701 tagtacattt atcccggaaa taaccggagc gattatcagg gtttaacaat attaaatacc
     8761 cgtagcgcat tactagcggg cctaaaacaa caatatggac atcttaacga tactctgcgc
     8821 ctgcgtgtgc ggaacagaaa cccttacccg atatacttgt tatgccagag gtagcaattg
     8881 cgttagcggc gtactgttgc tgtaaagtat aacgcctata ttttacagtt cagtcctctt
     8941 ttgcttatta caaccttaag tatatcaagc atataaagat aataagagac tgaacaatat
     9001 ggttacctgg acccaaatgt atatgccgat gggaggactg gggctatccg ctctggtcgc
     9061 cctgatcccg ataatattct tcttcgttgc actcgcggta ttacgtctga aaggacatgt
     9121 cgctggagca ataaccctta tattatctat cctgattgcg atattcgcct ttaaaatgcc
     9181 gattgatatg gcatttgctg ctgcgggcta tggctttatt tatggattat ggccaattgc
     9241 gtggattatt gtcgcggcgg tgttcctgta taaattaacc gttgccagcg ggcagttcga
     9301 tattatccgc agctcggtta tctccatcac cgacgatcag cgtttgcagg tgttactgat
     9361 tggtttctcc tttggtgcgt tgctggaagg agctgctggc tttggtgcga cggtggcgtt
     9421 tggtgcgttg ggcgtgccga ttctggtcgc cggtcaggta acgggaatcg atccgttcca
     9481 cattggcgca atggcgggac gtcagttacc gttcctgtcg gttcttgtgc cgttctggct
     9541 ggtagcaatg atggacggct ggaaaggggt gaaagagacg tggccagcgg cgctggttgc
     9601 tgggggaagc ttcgctgtca ctcagttctt tacctctaac tatattggtc cggaactgcc
     9661 ggatattact tcggcgctgg tgagtatcgt ctcactcgct ttatttctta aagtctggcg
     9721 gccgaaaaat accgaaacgg caatcagcat ggggcaatcc gcaggtgcga tggtggtaaa
     9781 taagccatct tctggcggtc ccgtgccttc agaatatagt ctggggcaaa tcattcgtgc
     9841 gtggtcaccg tttttaatct taacggtgct ggtcaccatc tggaccatga agccgtttaa
     9901 agcgttattt gctccgggcg gcgcgtttta ttcactggtg attaatttcc agatccctca
     9961 tttgcatcaa caagtgttga aagcggcacc cattgtcgcc caaccaacgc caatggatgc
    10021 ggtgtttaaa ttcgaccctc tctcggctgg cggcaccgct atttttattg cggcgattat
    10081 ctctatcttc atcctcggcg tgggcatcaa gaaagggatt ggcgtctttg ccgaaatgct
    10141 aattagtctg aagtggccga tattgtcgat tggcatggtg ctggcattcg ccttcgtcac
    10201 caactattcc ggcatgtcca ccacgctggc gctggtactg gcaggtacag gcgtgatgtt
    10261 cccgtttttc tcaccgtttc tcggctggct gggcgtattc cttaccgggt cggacacttc
    10321 ttcaaacgcc ctgtttggtt cactgcaatc gaccacggcg cagcaaatca acgtctctga
    10381 cactctgctg gtggcagcaa ataccagcgg cggcgtaact ggcaagatga tctccccgca
    10441 atctatcgcc gtggcctgcg ccgcgacggg catggtgggc cgagaatctg aactgttccg
    10501 ctacaccgtg aagcacagtc tgatttttgc cagcgttatc ggcgttatca ccctgctgca
    10561 ggcgtatgtg tttaccggga tgttagtctc gtaaatcaat aaagccggat gaaggtaaat
    10621 atcacccggc tttccatttg tctgtcgttt atctcgccag ggaaccgttt ttacgattgg
    10681 cttctttata cgtagtctcc gttttacgat gcgaattgtt taatatttat acaacttggc
    10741 ttctgtgctg gttataaagt acccactttc atgagatcaa ttttagtatg ttattacatt
    10801 gcttcatctg ttgctttcat gacgaatggc gttttttctc ttatttatat ttttcactag
    10861 aacaatgagt agatcaaaat tcccaaaact cccaaaaaca taagattatt cgcaaagctg
    10921 attttgcaga ataatgagaa cttttaagat ttgtcgagga tcaattttaa gcaatttatc
    10981 tccacttgtt acataaaaga tgccgttagg ttaataagtt aatcttgcta taacagtgtt
    11041 cgttactgcc tattaataaa gatatttggg atagggtaac gtggatgagg attcgaaatc
    11101 atccgtcttg cggaaagtga aatgttggca gatgttaata ttatgtatgt attttaactc
    11161 caacaacaca ccaatcattt tgttagaaat aagaaaattt atggttaaat aatttggtga
    11221 ttatgaagca ttaaatcgta atgccaaata ttgttcgctt tggagagtat tattgcctgt
    11281 ttgtgtctgt tgagcagaaa aatcatctga atttgaatac ttttattggg tgtgaataaa
    11341 tgaaaaaatc attaaaatca ttaaaatcat taaaatcatt aaaatcatta aaatcattaa
    11401 aatcattaaa atcattaaaa tcattaaaat cattaaaatc attaacttat atgaatattt
    11461 cagagttgga gttcgaatgt tgacacctga gtatttcctg ctaaaaatag ccctgcttta
    11521 cggatggttg tattgcaata acacgaaagt agtaagagat taaaggtatc tgtcagcgat
    11581 aactctgcaa gtgatgacat tttgtaaacc aaataatgta aatcagcaat gtatcttttc
    11641 acttaattat aacaaatgtg gtattcacta agaatctgat acgagcttac atcacgacta
    11701 ttttagtctc atcgcgggat acagggatgc accgatggat ctcagcacac cgtaatgcaa
    11761 tacctgaaca aatagaacag taaagtggtt cagtattaat gaaagctctt attttccgtt
    11821 gggaaaactt aaaatagatg agaaggcttt ttgatttctt atgtgatctc atttcgagta
    11881 ttgagaaatt tgtgattttt aaatttttca ataagccctg taagggattg gggttttatg
    11941 agaaataaag agacgtattg tctttgttta aacggtacg
//
LOCUS       DAJTRB010000114        11924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTRB010000114 DAJTRB010000000
VERSION     DAJTRB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.114"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..2341)
                     /locus_tag="OO330_003492"
     CDS             complement(<1..2341)
                     /locus_tag="OO330_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5884233.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEEMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="OO330_003493"
     CDS             complement(2275..2898)
                     /locus_tag="OO330_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5884234.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="OO330_003494"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="OO330_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO330_003495"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO330_003495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5884235.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="OO330_003496"
     CDS             7506..8228
                     /locus_tag="OO330_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5884236.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="OO330_003497"
     CDS             complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="OO330_003497"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5884237.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10300..11895)
                     /locus_tag="OO330_003498"
     CDS             complement(10300..11895)
                     /locus_tag="OO330_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5884238.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3306 c         2946 g         2915 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatct gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagaccgt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatctcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tcaggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccggcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt
    11821 ttcattttct ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ccccaccgct
    11881 ggcattattg gtcatatccg aaaaaaccag ccattgccca tcgg
//
LOCUS       DAJTRB010000115        11650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTRB010000115 DAJTRB010000000
VERSION     DAJTRB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.115"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            597..794
                     /locus_tag="OO330_003499"
     CDS             597..794
                     /locus_tag="OO330_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884239.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO330_003500"
     CDS             complement(744..1937)
                     /locus_tag="OO330_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5884240.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO330_003501"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO330_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5884241.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO330_003502"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO330_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5884242.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO330_003503"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO330_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5884243.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO330_003504"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO330_003504"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5884244.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO330_003505"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO330_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5884245.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO330_003506"
     CDS             complement(10573..10716)
                     /locus_tag="OO330_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884246.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11650
                     /locus_tag="OO330_003507"
     CDS             10966..>11650
                     /locus_tag="OO330_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5884247.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLF"
BASE COUNT         2955 a         2895 c         2989 g         2811 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaatagg aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg
//
LOCUS       DAJTRB010000116        11630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTRB010000116 DAJTRB010000000
VERSION     DAJTRB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.116"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            376..815
                     /locus_tag="OO330_003508"
                     /pseudo
     CDS             376..815
                     /locus_tag="OO330_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="OO330_003509"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="OO330_003509"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5884248.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="OO330_003510"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="OO330_003510"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5884249.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="OO330_003511"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="OO330_003511"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5884250.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGKRSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="OO330_003512"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="OO330_003512"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5884251.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="OO330_003513"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="OO330_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5884252.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="OO330_003514"
     CDS             complement(8300..11167)
                     /locus_tag="OO330_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5884253.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         2824 a         2844 c         2881 g         3081 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagccgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtaccatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gactttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaac tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttcgg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg gcgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctaattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg tttaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccga cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac aggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc cccttacaaa ggtgagttta tatcgagtta ttccctaagt tgtcgttact
    11401 tattccaatt cgttactatg aatatggcaa aaatattaca acaaacagcg gttaaaaaat
    11461 ttggtgaaga ttttttgtaa taatcctaat tagttatggg attttcgctt gatgtatctg
    11521 cataacaaaa acaatatgcg ccatttttgc attactaata agaaaaagca aaaaacatcg
    11581 atattcattt ttgttcaata gattttctgg cctatcgtta ttattgtgct
//
LOCUS       DAJTRB010000117        11623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTRB010000117 DAJTRB010000000
VERSION     DAJTRB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.117"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(282..357)
                     /locus_tag="OO330_003515"
     tRNA            complement(282..357)
                     /locus_tag="OO330_003515"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="OO330_003516"
     CDS             916..3168
                     /locus_tag="OO330_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884254.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4481
                     /gene="csiD"
                     /locus_tag="OO330_003517"
     CDS             3504..4481
                     /gene="csiD"
                     /locus_tag="OO330_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5884255.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4501..5769
                     /gene="lhgO"
                     /locus_tag="OO330_003518"
     CDS             4501..5769
                     /gene="lhgO"
                     /locus_tag="OO330_003518"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5884256.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5792..7240
                     /gene="gabD"
                     /locus_tag="OO330_003519"
                     /pseudo
     CDS             5792..7240
                     /gene="gabD"
                     /locus_tag="OO330_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7248..8534
                     /gene="gabT"
                     /locus_tag="OO330_003520"
     CDS             7248..8534
                     /gene="gabT"
                     /locus_tag="OO330_003520"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR5884257.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8772..10172
                     /gene="gabP"
                     /locus_tag="OO330_003521"
     CDS             8772..10172
                     /gene="gabP"
                     /locus_tag="OO330_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5884258.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10193..10855
                     /gene="csiR"
                     /locus_tag="OO330_003522"
     CDS             10193..10855
                     /gene="csiR"
                     /locus_tag="OO330_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5884259.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OO330_003523"
     CDS             complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OO330_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5884260.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11389..11547)
                     /locus_tag="OO330_003524"
     CDS             complement(11389..11547)
                     /locus_tag="OO330_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5884261.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2991 a         2852 c         2900 g         2880 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc
     4081 tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc
     4141 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     4201 ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt
     4261 ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc
     4321 tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg
     4381 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     4441 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     4501 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     4561 attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     4621 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     4681 ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg cgatcaaaac
     4741 ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct cgaaatggaa
     4801 cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga gtggataaac
     4861 gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat ttttgtgccg
     4921 tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat cttccaggcc
     4981 agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     5041 gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc
     5101 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     5161 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     5221 atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc
     5281 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     5341 aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg gattcgccgg
     5401 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     5461 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     5521 tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt
     5581 ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     5641 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     5701 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     5761 aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg
     5821 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt
     5881 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cagatgaaac
     5941 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa
     6001 agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc aggacgattt
     6061 agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag gcgaaatcag
     6121 ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt atggcgacac
     6181 cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga ttggcgtcac
     6241 cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag ccggtccggc
     6301 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     6361 ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac
     6421 cggttcggca ggcgcggtcg gtaatgaact gaccagcaac ccgctggtac gcaaactgtc
     6481 gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     6541 aaaagtgtcg ctggagctgg gcggcaacgc gcagtttatc gtctttgacg atgccgatct
     6601 cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     6661 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca
     6721 gcaggcggtg agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc
     6781 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     6841 aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc
     6901 aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     6961 cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag ccaatgacac
     7021 cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtcgg
     7081 cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     7141 cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     7201 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaaggaaca
     7261 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     7321 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     7381 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     7441 tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     7501 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     7561 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     7621 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac gggcgcacgc
     7681 attacaccct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     7741 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     7801 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     7861 tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc
     7921 agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     7981 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg gcgccggatc
     8041 ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg
     8101 cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc ggtaacccga
     8161 ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa
     8221 aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc
     8281 cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg tttgaagatg
     8341 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     8401 agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccac
     8461 tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag tgttttgatg
     8521 aggcgaagca gtagcaccgc ccccatgcag gaggcgacgc tgcgcgtctt gtccggccta
     8581 cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga
     8641 ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa tcaataacaa
     8701 ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     8761 gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg ctgaaatcac
     8821 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     8881 gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc gccggattac
     8941 tggtggttat gattatgcgg atgctggcgg aaatggcagt tgccacgccc gataccggtt
     9001 cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc atcggctggt
     9061 tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc gccatgatcc
     9121 tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     9181 taactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     9241 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     9301 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     9361 ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     9421 cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat attgtccgcg
     9481 ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtcg
     9541 tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc
     9601 tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata ttactttccg
     9661 taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac tccttaagcc
     9721 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg
     9781 cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat tacgcgcctg
     9841 cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag
     9901 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     9961 gcttgcgaat gtggcttgat ccctggctca cctggctggt catcggcttt attacctttg
    10021 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
    10081 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
    10141 ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat
    10201 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
    10261 ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg ttgggccgtt
    10321 gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
    10381 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
    10441 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
    10501 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
    10561 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
    10621 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
    10681 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
    10741 ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg agttaatgcg
    10801 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
    10861 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
    10921 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
    10981 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
    11041 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
    11101 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
    11161 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
    11221 tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat
    11281 ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
    11341 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
    11401 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
    11461 aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg gcagaatgat
    11521 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
    11581 aaaaagagat tttatctctt aagcgtagta attttagcgg agc
//
LOCUS       DAJTRB010000118        11499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTRB010000118 DAJTRB010000000
VERSION     DAJTRB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.118"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..562
                     /gene="ipgB1"
                     /locus_tag="OO330_003525"
     CDS             <1..562
                     /gene="ipgB1"
                     /locus_tag="OO330_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5884262.1"
                     /translation="LSRNKLVKKILSVFNLKQRFPQKNFGCPVNINKIRDSVIDKIKD
                     SNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGVVLTSDNKKNIFAAIEKKFPDIK
                     LDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMDLFNTQMKDLTNLVSSSVYDKIF
                     NESTKVLQIEISAEVLKAVYRQSNTN"
     gene            619..1086
                     /gene="ipgC"
                     /locus_tag="OO330_003526"
     CDS             619..1086
                     /gene="ipgC"
                     /locus_tag="OO330_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5884263.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            1092..2834
                     /gene="ipaB"
                     /locus_tag="OO330_003527"
     CDS             1092..2834
                     /gene="ipaB"
                     /locus_tag="OO330_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5884264.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            2854..3945
                     /gene="ipaC"
                     /locus_tag="OO330_003528"
     CDS             2854..3945
                     /gene="ipaC"
                     /locus_tag="OO330_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5884265.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            3996..4994
                     /gene="ipaD"
                     /locus_tag="OO330_003529"
     CDS             3996..4994
                     /gene="ipaD"
                     /locus_tag="OO330_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5884266.1"
                     /translation="MNITTLTNSISTPSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLNILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLENLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            5003..6904
                     /gene="ipaA"
                     /locus_tag="OO330_003530"
     CDS             5003..6904
                     /gene="ipaA"
                     /locus_tag="OO330_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5884267.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            6908..7144
                     /locus_tag="OO330_003531"
     CDS             6908..7144
                     /locus_tag="OO330_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5884268.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            7361..8290
                     /gene="virB"
                     /locus_tag="OO330_003532"
     CDS             7361..8290
                     /gene="virB"
                     /locus_tag="OO330_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5884269.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(9237..10016)
                     /gene="ipaJ"
                     /locus_tag="OO330_003533"
     CDS             complement(9237..10016)
                     /gene="ipaJ"
                     /locus_tag="OO330_003533"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5884270.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(10616..10753)
                     /locus_tag="OO330_003534"
     CDS             complement(10616..10753)
                     /locus_tag="OO330_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884271.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            11369..>11499
                     /locus_tag="OO330_003535"
     CDS             11369..>11499
                     /locus_tag="OO330_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5884272.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     "
BASE COUNT         4130 a         2359 c         1861 g         3149 t
ORIGIN      
        1 gttaagtcgc aataagcttg taaagaaaat attatctgtt tttaacttaa aacaacgctt
       61 tcctcagaag aattttggat gcccggtgaa tataaataaa atcagggaca gcgtcataga
      121 caaaataaaa gatagtaatt cggggaatca actattttgt tggatgagcc aagagcgaac
      181 cacttatgtc tcttcaatga taaacagaag tatagatgaa atggccatac acaatggagt
      241 tgttttgacc tcagataaca aaaaaaacat atttgcagct attgaaaaaa aattcccgga
      301 tataaaactt gatgaaaaat cagcacaaac atctatcagt catacagcac taaacgagat
      361 tgcctcatct ggcctcagag caaagatttt aaaacgctat agcagtgaca tggatttgtt
      421 taacacacaa atgaaggatc ttacaaatct agtatccagt tccgtctacg ataaaatatt
      481 taatgaatca acaaaagttt tacaaataga aataagcgca gaggttctaa aggctgtata
      541 ccgtcaaagc aatacaaatt aaatctttta tattaaatct tatacttatt aaagtaaagc
      601 agacaaaaag gagaccttat gtctttaaat atcaccgaaa atgaaagcat ctctactgca
      661 gtaattgatg caattaactc tggcgctaca ctgaaagata ttaatgcaat tcctgatgat
      721 atgatggatg acatttattc atatgcttat gacttttaca acaaaggaag aatagaggaa
      781 gctgaagttt tcttcaggtt tttatgtata tacgactttt acaatgtaga ctacattatg
      841 ggactcgcag ctatttatca gataaaagaa cagttccaac aagcagcaga cctttatgct
      901 gtcgcttttg cattaggaaa aaatgactat acaccagtat tccatactgg acaatgccag
      961 cttcggttga aagccccctt aaaagctaaa gagtgcttcg aactcgtaat tcaacacagc
     1021 aatgatgaaa aattaaaaat aaaagcacaa tcatacttgg acgcaattca ggatatcaag
     1081 gagtaattat tatgcataat gtaagcacca caaccactgg ttttcctctt gccaaaatat
     1141 tggcttccac tgagcttgga gacaatacta tccaagctgc aaatgatgca gctaacaaat
     1201 tattttctct tacaattgct gatcttactg ctaaccaaaa tattaataca actaatgcac
     1261 actcaacttc aaatatatta atccctgaac ttaaagcacc aaagtcatta aatgcaagtt
     1321 cccaactaac gcttttaatt ggaaacctta ttcaaatact cggtgaaaaa tctttaactg
     1381 cattaacaaa taaaattact gcttggaagt cccagcaaca ggcaagacag caaaaaaacc
     1441 tagaattctc cgataaaatt aacactcttc tatctgaaac tgaaggacta accagagact
     1501 atgaaaaaca aattaataaa ctaaaaaacg cagattctaa aataaaagac ctagaaaata
     1561 aaattaacca aattcaaaca agattatccg aactcgaccc agagtcacca gaaaagaaaa
     1621 aattaagccg ggaagaaata caactcacta tcaaaaaaga cgcagcagtt aaagacagga
     1681 cattgattga gcagaaaacc ctgtcaattc atagcaaact tacagataaa tcaatgcaac
     1741 tcgaaaaaga aatagactct ttttctgcat tttcaaacac agcatctgct gaacagctat
     1801 caacccagca gaaatcatta accggacttg ccagtgttac tcaattgatg gcaaccttta
     1861 ttcaactagt tggaaaaaat aatgaagaat ctttaaaaaa tgatctggct ctattccagt
     1921 ctctccaaga atcaagaaaa actgaaatgg agagaaaatc tgatgagtat gctgctgaag
     1981 tacgtaaagc agaagaactc aacagagtaa tgggttgtgt tgggaaaata cttggggcac
     2041 ttttaactat cgttagtgtt gttgcagcag ctttttctgg aggagcctct ctagcactgg
     2101 cagctgttgg tttagctctt atggttacgg atgctatagt acaagcagcg accggcaatt
     2161 ccttcatgga acaagccctg aatccgatca tgaaagcagt cattgaaccc ttaatcaaac
     2221 tcctttcaga tgcatttaca aaaatgctcg aaggcttggg cgtcgactcg aaaaaagcca
     2281 aaatgattgg ctctattctg ggggcaatcg caggcgctct tgtcctagtt gcagcagtcg
     2341 ttctcgtagc cactgttggt aaacaggcag cagcaaaact tgcagaaaat attggcaaaa
     2401 taataggtaa aaccctcaca gaccttatac caaagtttct caagaatttt tcttctcaac
     2461 tggacgattt aatcactaat gctgttgcca gattaaataa atttcttggt gcagcgggtg
     2521 atgaagtaat atccaaacaa attatttcca cccatttaaa ccaagcagtt ttattaggag
     2581 aaagtgttaa ctctgccaca caagcgggag gaagtgtcgc ttctgctgtt ttccagaaca
     2641 gcgcgtcgac aaatctagca gacctgacat tatcgaaata tcaagttgaa caactgtcaa
     2701 aatatatcag tgaagcaata gaaaaattcg gccaattgca ggaagtaatt gcagatctat
     2761 tagcctcaat gtccaactct caggctaata gaactgatgt tgcaaaagca attttgcaac
     2821 aaactactgc ttgatacaaa taaggagaat gttatggaaa ttcaaaacac aaaaccaacc
     2881 cagattttat atacagatat atccacaaaa caaactcaaa gttcttccga aacacaaaaa
     2941 tcacaaaatt atcagcagat tgcagcgcat attccactta atgtcggtaa aaatcccgta
     3001 ttaacaacca cattaaatga tgatcaactt ttaaagttat cagagcaggt tcagcatgat
     3061 tcagaaatca ttgctcgcct tactgacaaa aagatgaaag atctttcaga gatgagtcac
     3121 acccttactc cagagaacac tctggatatt tccagtcttt cttctaatgc tgtttcttta
     3181 attattagtg tagccgttct actttctgct ctccgcactg cagaaactaa attgggctct
     3241 caattgtcat tgattgcgtt cgatgctaca aaatcagctg cagagaacat tgttcggcaa
     3301 ggcctggcag ccctatcatc aagcattact ggagcagtca cacaagtagg tataacgggt
     3361 atcggtgcca aaaaaacgca ttcagggatt agcgaccaaa aaggagcctt aagaaagaac
     3421 cttgccactg ctcaatctct tgaaaaagag cttgcaggtt ctaaattagg gttaaataaa
     3481 caaatagata caaatatcac ctcaccacaa actaactcta gcacaaaaat tttaggtaaa
     3541 aataaactgg cgccagataa tatatccctg tcaactgaac ataaaacttc tcttagttct
     3601 cccgatattt ctttgcagga taaaattgac acccagagaa gaacttacga gctcaatacc
     3661 ctttctgcgc agcaaaaaca aaacattggc cgtgcaacaa tggaaacatc agccgttgct
     3721 ggtaatatat ccacatcagg agggcgttat gcatctgctc ttgaagaaga agaacaacta
     3781 atcagtcagg ccagcagtaa acaagcagag gaagcatccc aagtatctaa agaagcatcc
     3841 caagcgacaa atcaattaat acaaaaatta ttgaatataa ttgacagcat caaccaatca
     3901 aagaattcga cagccagtca gattgctggt aacattcgag cttaatatat ccaagagcca
     3961 taataatata tggctcttcc tgtaaggaaa taaccatgaa tataacaact ctgactaata
     4021 gtatttccac cccatcattc agtccaaaca ataccaacgg ttcatcaacc gaaacagtta
     4081 attctgatat aaaaacaacg accagttctc atcctgtaag ttcccttact atgctcaacg
     4141 acacccttca taatatcaga acaacaaatc aggcattaaa gaaagagctt tcacaaaaaa
     4201 cgttgactaa aacatcgcta gaagaaatag cattacattc atctcagatt agcatggatg
     4261 taaataaatc cgctcaacta ttgaatattc tttccaggaa cgaatatcca attaataaag
     4321 acgcaagaga attattacat tcagctccga aagaagccga gcttgatgga gatcaaatga
     4381 tatctcatag agaactgtgg gctaaaattg caaactccat caataatatt aatgaacagt
     4441 atctgaaagt atatgaacat gccgttagtt catatactca aatgtatcaa gattttagcg
     4501 ctgttctttc cagtcttgcc ggctggatct ctcccggagg taacgacgga aactccgtga
     4561 aattacaagt caactcgctt aaaaaggcat tggaagaact caaggaaaaa tataaagata
     4621 aaccgctata tccagcaaat aatactgtta gtcaggaaca agcaaataaa tggcttacag
     4681 aattaggtgg aacaatcggc aaggtatctc aaaaaaacgg gggatatgtt gtcagtataa
     4741 acatgacccc aatagacaat atgttaaaaa gcttagagaa tctaggtgga aatggcgagg
     4801 ttgtgctaga taatgcaaaa tatcaggcat ggaatgccgg attctctgcc gaagatgaaa
     4861 caatgaaaaa caatcttcaa actttagttc aaaaatacag taatgccaat agtatttttg
     4921 ataatttagt aaaggttttg agtagtacaa taagctcatg tacagataca gataaacttt
     4981 ttctccattt ctgaggtgcg ccatgcataa tgtaaataat actcaagcgc caacattctt
     5041 atataaggca acttcaccat catcaacaga atacagcgag ttaaaaagca aaatatccga
     5101 tatccatagt tcgcaaactt ctctaaaaac accagcatca gtgtctgaaa aagaaaactt
     5161 tgcaacgtct tttaatcaga aatgtcttga ttttttattt tcttcctcag ggaaagaaga
     5221 tgtgttaaga agcatttatt ccaactcaat gaatgcgtat gccaaaagcg agattctcga
     5281 attttcaaat gttttgtact ccttagtaca tcaaaatggt cttaattttg aaaacgaaaa
     5341 gggacttcaa aaaattgtcg cacagtattc ggaactaatt ataaaagata aattatccca
     5401 agattctgcc tttggaccat ggtcggcaaa gaataagaaa ctccatcaat tacgacaaaa
     5461 cattgagcac agacttgcac tattagcaca acaacacaca tctggtgaag ctttatcatt
     5521 gggacaaaaa ctcctcaata ctgaagtatc atcatttatc aagaataata ttcttgctga
     5581 attaaagtta agtaatgaaa ctgtttcatc tctcaaacta gatgatttag ttgacgcaca
     5641 ggcaaaactt gcctttgata gtttgcgcaa tcaacgtaaa aatactattg atagtaaagg
     5701 atttggtata ggtaaactgt caagagactt aaatacagta gccgtgtttc ctgagctgtt
     5761 gagaaaagtc cttaatgata ttttagaaga tataaaagat tcgcatccta tccaagatgg
     5821 cctccctaca cctcccgaag atatgccaga tggcggacca acccccggag ccaatgagaa
     5881 aacatcccaa cctgtaattc actatcatat aaataatgat aatagaactt acgataatag
     5941 agtttttgac aacagagtat atgacaatag ctatcacgag aacccagaaa atgatgcaca
     6001 gtctcctact tctcagacaa acgatctatt atcccgtaac ggaaactcat tactaaatcc
     6061 acaaagagca ctagttcaaa aagtaacttc cgttctacca cactctatat cagatactgt
     6121 ccagacattt gcaaataatt cagctttaga aaaggttttc aaccatactc cagataattc
     6181 ggatggaata ggttcagacc tgttaactac gagtagtcaa gaaagatctg caaataactc
     6241 tctttctcgg ggacacaggc ctctgaacat acagaactct tcaaccaccc cccctctcca
     6301 cccggaagga gtgacaagca gtaatgataa ctcatcagat acaactaaaa gtagcgcttc
     6361 tctttctcat agagtagctt cgcaaatcaa taaattcaac tcaaacactg attcaaaagt
     6421 acttcagact gattttttat caagaaatgg agacacatat ttaacacggg aaacgatatt
     6481 tgaagcttca aaaaaagtaa caaactccct aagtaatctt atatctctca ttggaactaa
     6541 atcaggaaca caagaacgag agttacagga aaaatcaaag gacattacaa aatccacaac
     6601 agaacataga ataaacaaca aattaaaagt tacagatgca aatataagaa actacgtaac
     6661 agaaaccaac gcagatacaa ttgataaaaa tcatgcgatc tatgaaaagg caaaagaagt
     6721 atctagcgcc ctcagcaagg tattgtcaaa aattgacgat acctctgcag aattacttac
     6781 agatgatata tctgatttaa aaaataacaa tgatattaca gctgaaaaca ataatatata
     6841 taaagcagca aaagatgtaa ccacttccct atcaaaagta ttaaagaata tcaataagga
     6901 ttaaaatatg ataaaagaaa aaatattatc aatagtggcc ttttgctatg gtatagcgta
     6961 cagcaaacta tctgaagaaa ctaaatttat tgaagatctt tctgccgatt ctctttctct
     7021 gattgaaatg ctggatatga ttagttttga gttcaatcta cgtatagatg aatctacatt
     7081 agaacacatt attactattg gagatcttat ttctgtagtc aaaaatagta caaaatcaat
     7141 atagtaattg ataagcattt tttcatctat ggagctctca catcagagct ccacaagaat
     7201 attattcttt tatccaataa agataaattg catcaatcca gctattaaaa tagtaatttt
     7261 taagactacc gttgactatc atcaacagtt acatacaatc ttgctcacac tgcatttaac
     7321 ttttgtcaat ataaatcaat atgaataaac agggtgtgat atggtggatt tgtgcaacga
     7381 cttgttaagt ataaaggaag gccaaaagaa agagtttaca ctccattctg gtaataaagt
     7441 ttcctttatc aaagccaaga ttcctcataa aaggatccaa gatttaacct tcgtcaacca
     7501 aaaaacgaat gtacgcgatc aagaatccct aacagaagaa tcattagccg atatcataaa
     7561 aactataaag ctacaacaat tcttccctgt aataggaagg gagattgatg gtagaattga
     7621 aattctggat ggcactcgta gaagagcatc tgcaatatat gcaggagcag atcttgaagt
     7681 tctatattca aaagaatata tatctactct tgatgccaga aaactagcaa acgatataca
     7741 aacagcaaaa gagcatagca tccgagaact tggtattggt cttaattttc tgaaagtatc
     7801 agggatgtcc tataaagaca tagccaaaaa agagaatctg tctcgcgcga aagtcactcg
     7861 tgcctttcag gcagcaagcg ttccacagga aataatatct ctatttccaa tcgcgtcaga
     7921 acttaacttt aatgactaca agatattatt caattattat aaaggacttg aaaaggctaa
     7981 tgaatctctt agttctacac taccaatatt aaaggaagaa ataaaagacc ttgatacaaa
     8041 tttgcccccg gacatatata aaaaagaaat tttaaacatc ataaagaaaa gcaaaaacag
     8101 aaagcaaaac ccttcgctaa aagttgactc gttatttatt tctaaagaca aacggactta
     8161 cataaaaaga aaagagaata aaacaaacag aactctaata tttacattat ctaaaataaa
     8221 caaaacagtt cagagagaaa tagatgaagc tattcgggat ataatttctc gccatctatc
     8281 gtcttcataa acatcgaaca ctgatgtaaa ctgcccaatt cagataataa aaatcttcgt
     8341 tgaacttgac tttcagccgg tggggtaatt ccgtcggcta tctgttattc aatccccacg
     8401 cactgtgttt ccttaaaatt acggtgaaaa ctcataccct gctgaccacg agaaaattat
     8461 ctggtgaaat tagtaatatt catatcattg aaaatgaatt gtgcaactaa gggatgcggc
     8521 atgatggtca aaattttata tgaagatgag tgactggccg aaaatattca gcgtattttt
     8581 aatcgtttca ataaaaacag cgaggattag cagtcaacgc ttttactact caattttttt
     8641 tacatggtga taaacaaaaa acccaccatt aacagacttc gttcacagta tcaaaaaagc
     8701 tttaagaata tattagcaga tttctcaaat ataattactg ttttattctc ctaatcttcc
     8761 cataaaatga ccgacttaca ttattttata attataaaca agtttgattt taaaatcata
     8821 ttaattatta acaagagatt tatttttcta accccaaaag ttatgcttag tcaaagagta
     8881 acttataata tccatacccc tcatagggct tacagtttaa attgcgggta taaaatacat
     8941 ctattttcaa ataaaataaa gcatactcca attacaacga gtgactaaaa taaaaatatc
     9001 atccaagtca atcactgttt gctctgcaaa atatgtcagc cttctcaaac ttaccagcac
     9061 ttaaatattt aagcacacaa tcattccttg agggagttca cttatttcaa gagaaacatt
     9121 tcgtcttccc acacaactaa tccgcccaat atagctaaat ttgtactcag taatatcatt
     9181 tgttatgccc cccgccccaa aatattcaag ctccttcaca gataaaaaac gacaatttac
     9241 aaagcctcat tagttataac tatggaaata cctattttag tatatcctat aaagttagaa
     9301 ttacttcttc ttgcacctaa ttccatagtt gaaaaacaag aataattttc tcctacagct
     9361 ggatccatat agcttccatc aggtctacac aacacccagt ggaggccgac aggaactcca
     9421 ataaaggata cagcaacagc ttccaaaact ctttggttag aagataacac atctcgttga
     9481 tgtactatat tacatcccat gccaattaat aagtcccttg catcgggata aataaaacta
     9541 attacttgcg aaaaaatgtt actttcttca acgacataag cattcaatcc aagaagcctc
     9601 gtagccatga ctatcttatc aggcatagaa taaccggcat ccgcgatatt atctttgtgt
     9661 attctcggat tataattacc actagttatt aagtataaat cccgctcaca acgattatcc
     9721 aaatcaagag aactcttcct tgtcatttcg gacatagacc ctttgtattg tggtatttta
     9781 tcaaccccta attcctttgc agcacagagt aaactggcag ctccacaaga attgtcatac
     9841 atctgcttcc tcgccaaaaa acaaccttgt tcgtttactc tattctcatt taccctcaca
     9901 tcagtctgat gagatatcat atattctctt ggtatagccc cctgcaataa aaccccatat
     9961 aacataacac ctgtatgaat acatccccgt ttgcaaggct tccgttgttc cgacatcgac
    10021 gagtaattgc agcacagtat tgcatccagc aatgcgaact actatgagaa ttagctgacc
    10081 gggtccttat tccccgaacg aactttaaat ccaaaacctc atactctttt ccgtcgtcat
    10141 cacgtccgtc accataacaa cctttcagca tccacccttt tattaagagt taatgtccat
    10201 cagcatcagc aattaatacc gcctgatact gacatatttt tgtcgtattt acgtccataa
    10261 tcggttattg tgatacaggt cacaccagct tatttgctat tgttacaata accaccttgg
    10321 ttgagagctg ttttttcaac tttgttatca actcccccaa ctccttggtc agtaaggctg
    10381 ccgaccgagc acagtgaata gatagtatcc gcaggtacca ccatgcttac ttaatactca
    10441 gaaggcgaag tgctctccaa ttaactctgc cgcactatgc ttataagctg cttttcaatg
    10501 ccgtctatca acatgtccag ttcattcaat ctgttgtact ggtaatccaa cgcagtaatg
    10561 atatatgcag gtattctttc atccactcca gtaacgtttc atgcaaagca ttaatttacg
    10621 cgctacggaa cttcaccagt tgactgagca aacgatgtgg gaccagtact gcctgcagtt
    10681 cttctattgt tacagcgatt tctttaccaa acagctgaac caccatcaag ataaagatag
    10741 cctgaacatt catcgcacca ttttgttacc aatggcaaaa gatttaacga accgtgcctg
    10801 catcaggcgg actttttatc cctgctttgt tagctcccta accttgtaca gagagcctcc
    10861 ataagctccc atatcaagca gacatggttc gcagttactg acatacacca gaaaactctg
    10921 acagcagact cacttatcct ccacttcact catatcctta ttggtgagat gaacctgaat
    10981 gacatgtttt gcgacataaa cgccaaccag tgtatatttc acctgtagag attctacaat
    11041 ctgtagcgtt atgcatctaa tgttaggctc tatgatgtca gaaatctgcg agactctaca
    11101 ccttctgcat ttacctttat ttatcaccat gctcctcagt taacggagaa cgtgttcatt
    11161 gcattttgtc acttacattt cgcctttggt atcgggggga tataggttcc aacgttccgc
    11221 atgagaacct gaaccggctt cacgccacct tgataccaga tgccgcacag gtagtcaaca
    11281 agtgtcacga acggtgcaat agtgatccac acccaacgcc tgaaatcaga tccagggggt
    11341 aatctgctct cctgattcag gagagcttat ggtcactttt gagacagtta tggaaattaa
    11401 aatcctgcac aagcagggaa tgagtagccg gacgattgcc agagaactgg ggctctcccg
    11461 caataccgtt aaacgttatt tgcaggcaaa atctgagcc
//
LOCUS       DAJTRB010000119        11456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTRB010000119 DAJTRB010000000
VERSION     DAJTRB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.119"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(58..1440)
                     /gene="cusC"
                     /locus_tag="OO330_003536"
     CDS             complement(58..1440)
                     /gene="cusC"
                     /locus_tag="OO330_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5884273.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRAWALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            1597..2280
                     /gene="cusR"
                     /locus_tag="OO330_003537"
     CDS             1597..2280
                     /gene="cusR"
                     /locus_tag="OO330_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5884274.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2270..3712
                     /gene="cusS"
                     /locus_tag="OO330_003538"
     CDS             2270..3712
                     /gene="cusS"
                     /locus_tag="OO330_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5884275.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            3862..6099
                     /gene="nrfB"
                     /locus_tag="OO330_003539"
     CDS             3862..6099
                     /gene="nrfB"
                     /locus_tag="OO330_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5884276.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            6086..9054
                     /gene="nfrA"
                     /locus_tag="OO330_003540"
                     /pseudo
     CDS             6086..9054
                     /gene="nfrA"
                     /locus_tag="OO330_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            9055..9945
                     /locus_tag="OO330_003541"
     CDS             9055..9945
                     /locus_tag="OO330_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5884277.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            10128..10889
                     /gene="envY"
                     /locus_tag="OO330_003542"
     CDS             10128..10889
                     /gene="envY"
                     /locus_tag="OO330_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5884278.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(10935..11011)
                     /locus_tag="OO330_003543"
     tRNA            complement(10935..11011)
                     /locus_tag="OO330_003543"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10975..10977),aa:Arg,seq:tct)
     gene            11254..>11456
                     /locus_tag="OO330_003544"
     CDS             11254..>11456
                     /locus_tag="OO330_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5884279.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIILKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         2799 a         2985 c         2931 g         2741 t
ORIGIN      
        1 gactgaacat tgcgacttgc agtgcttttt tcatttattc tctcctggag ttaaaaatta
       61 ttgctgccaa ccgcccccta aggcggtata caaataaatt tcgttaacct gacgggcata
      121 attcaggtca agtaaagttt gtcgggttgc aaataaagaa cgctcggcat ccagcacttc
      181 cagataactt accgcgccgt gctgatataa tgcccacgcc cgttgcaggg taatttgcag
      241 cgacgccaga taacgctgct gggcgctgat ttgatcgtcc aggctttgac gtaatgccag
      301 cgcatctgcc acctctttaa aggcgttctg gattttctgt tcataattca ccaccgactg
      361 ctgctggcga atttcggcga tatccagatt ggcctggttg cgtccggcat taaaaatggg
      421 gatctcaatt ttaggaataa aattccacat cccgctgcta gcgttaaata atgacgacag
      481 atcgctgctg gcggttgata ttccgctggt caggctgata gacgggaaaa atgccgcacg
      541 cgcagcacca atattagcat tagccgccat taacgcgtgt tcagcttcca taatatccgg
      601 gcgctgcaat aagatttgcg acgacaagcc cgccggtaat ttaacgcttt gcaggctgcc
      661 gctgtttact gtctgcgctt gcggtagctt gccgtagctt cccaataaca gttgcaatgc
      721 attattcgcc tgcgccagtt ccccctgacg tttagcgatg tcactgcggg tactttctat
      781 caccccgcga gcctgttcca gcgccagaac attgctgcta ccggtcaaca gttgtttttc
      841 gacaaacgca tatgactgct gataattacg cagcgtttct tcggctattt gcaattgcgc
      901 atacgccagc tgctgattga aatagctttg cgcgacatta gaaaccagca aaatatgcac
      961 cgcgcgctgg gcttcctcag tggctaaata attttggcgc tcggcttcgc tcatgttctt
     1021 taagcgaccg aaaaaatcga gatcaaagct ggcgttaagg ccagtcgaga actcccgcgt
     1081 cgtggctgaa tcgcctttaa gattgccgct ccagctgccg ctgccctcgc cattgagctg
     1141 tgggtagcgg tcggcatcgg tcagacgata ttgcgcccgt gcctcctgca ctttcagcgt
     1201 cgccatgcgc aaatcccggt tattcaccag cgcctcgcta atcagcgtct tcacctgatt
     1261 atcaacaaaa aaggtgcgcc agcccacgtt ctggtagtta tccgctgcgt taaccaggcc
     1321 gttctggctg agtgagaact gctgcggtac gggcattacc ggacgctgat aatccggtgc
     1381 cagtgaacaa ccagttagcg caagggccac acaaaatggc agaagtttac aaggagacat
     1441 aggctcataa tttctggtga ttttatgtcg ccaactttac tcgccaggct ctgattttcc
     1501 ggtgacagga aaatgacaaa attgtcattt tgccaataag cgattgccat ctgatcccgc
     1561 tactctagaa ttgcccgggc aacatgcgga ggaaatatga aactgttgat tgtcgaagat
     1621 gaaaagaaaa ccggagaata cttgaccaaa gggttaaccg aagccggttt tgtggtcgat
     1681 ttggccgaca acgggctgaa tggctaccat ctggcgatga ccggtgatta tgacctgata
     1741 atcctcgata ttatgctgcc agacgtgaac ggctgggata tcgtgcgcat gttgcgctcc
     1801 gccaataaag ggatgccgat tctgttgctt accgcgcttg gcaccattga acatcgcgtc
     1861 aaggggctgg agttgggggc agatgactac ctggtgaaac cgttcgcttt tgctgaactg
     1921 ctggcgcggg tgcgcactct cctgcggcgc ggggcggcgg tgattatcga aagtcagttt
     1981 caggttgccg atctgatggt cgatctcgtc agccgcaaag tcaccctcag cggcacgcgc
     2041 atcactctga ccagtaaaga atttactctg ctggagtttt ttctccgcca tcagggcgaa
     2101 gtgctgcccc gctcgcttat cgcctcgcag gtatgggaca tgaatttcga cagcgacact
     2161 aacgccattg atgtggcggt gaagcggcta cgcggcaaaa tcgacaacga ctttgagccg
     2221 aagctgattc aaaccgtgcg cggcgtgggt tacatgcttg aggtgccgga tggtcagtaa
     2281 gccatttcag cgcccgtttt cgctggcaac ccgcctgacc ttttttatca gcctggccac
     2341 catcgcggcg tttttcgcct ttgcatggat cataatccac tcagtaaaag tgcattttgc
     2401 cgagcaggac attaatgatt taaaagagat tagcgccacc cttgaacggg tactaaatca
     2461 ccctgacgaa acgcaagccc gacgcttaat gacgctggaa gatatcgtca gtggttattc
     2521 caacgtgttg atttccctgg cagatagcca cggcaaaacg gtgtatcact cccccggtgc
     2581 gcctgatatc cgcgagtttg cacgtgatgc catacccgat aaagacgcgc ggggtggcga
     2641 ggtgtatctc ctttccggcc cgacgattat gatgccaggc cacggtcacg ggcatatgga
     2701 acacagcaac tggcggatga ttaacttgcc ggttggcccg ttggtggacg gcaaaccgat
     2761 ttatacgctc tacatcgcgc tgtcgatcga ttttcatctt cattacataa atgatttgat
     2821 gaataaactt attatgaccg catcggtaat cagcatcctg atcgtcttta tcgtgctgtt
     2881 ggcggtacat aaaggtcacg cgccgatccg cagcgtcagc cgtcaaatcc agaatatcac
     2941 ctcgaaagat ctcgacgttc gccttgaccc gcagaccgtg ccgattgagc tggaacagct
     3001 ggtactgtcg ttcaaccata tgatcgagcg tattgaggat gtctttactc gccagtccaa
     3061 tttctcagcg gatatcgccc acgaaattcg cacaccaatt actaatctca ttacgcaaac
     3121 ggaaatcgcc ctcagccagt cgcgcagcca gaaggagctg gaagatgtgc tctactctaa
     3181 tctcgaagag ctgacgcgaa tggcgaaaat ggtcagcgat atgctgtttc tcgctcaggc
     3241 cgataacaac cagctaatcc cggaaaagaa aatgctcaac ctggcggatg aagtcggcaa
     3301 agtgttcgat tttttcgagg cgctagcgga agatcgcggc gtggagttgc ggtttgttgg
     3361 cgacaaacgt caggttgcgg gcgatccgtt gatgctgcgt cgggcgctaa gcaacctgct
     3421 ttctaatgcc cttcgttata cgccaaccag agagacaatt gtagtacgct gtcagacggt
     3481 cgatcaccag gtgcaagttt ccgtcgaaaa ccccggtacg cccattgcgc ccgagcactt
     3541 accgcgattg tttgaccgtt tctatcgcgt tgacccctcc cgccagcgaa aaggtgaagg
     3601 tagcggtatt gggctggcga tagtgaaatc gattgttgtc gcgcataaag gcacggttgc
     3661 ggtaacgtca gatgcgcggg ggacaaggtt tgttatcaca ttacccgctt aattattcag
     3721 caaacggcaa cttttataac cagtgtaaaa ataacgtgcc gcaatagtca tacatattaa
     3781 tggtaaaaag ctgtcacaat tcataaaaaa ccttaatata cgccacccta aacataacca
     3841 gcgttaatgt aaggtttttg tgtggactgg cttcttgatg tttttgctac ctggctctac
     3901 ggcttaaaag taatcgcgat aacgttagcg gtcatcatgt tcatcagcgg gctggacgat
     3961 ttttttattg atgtcgtcta ctgggtacgc cgcattaaac gcaagttgag tgtttatcgc
     4021 cgctacccgc gaatgagtta ccgcgaactg tataaaccag atgaaaaacc gttagcgatt
     4081 atggttccgg cgtggaatga aacgggcgtc atcggcaata tggccgagct ggcggcgacc
     4141 acgctcgact acgaaaacta tcatatcttt gttggcacct accccaacga ccccgatact
     4201 caacgtgatg ttgacgaagt gtgcgctcgc tttccgaacg tgcataaggt agtctgcgcg
     4261 cgtcctggtc ccaccagcaa agccgactgc ctgaacaacg tgctggacgc catcacccag
     4321 tttgaacgta gcgccaattt cgcttttgct ggttttattc tgcatgacgc cgaagatgtg
     4381 atttcaccga tggaattgcg tctgttcaac tatctggtcg agcgtaaaga tctgattcag
     4441 atcccagtgt atccgtttga acgcgaatgg acgcacttca ccagcatgac ttacattgat
     4501 gagttttcag aactgcatgg caaagatgtt ccggtgcgtg aagccctcgc cggacaagtg
     4561 cccagcgcag gcgtcggcac ctgtttcagc cgccgcgccg tgaccgcact gttagctgac
     4621 ggtaacggta ttgctttcga cgtgcagagt cttactgaag attacgacat tggcttccgc
     4681 ctgaaagaaa aaggtatgac ggaaattttt gtccgttttc cggtggtgga cgaagccaaa
     4741 gaacgcgagc agcgtaaatt tttacagcac gcacggacgt caaacatgat ctgcgtgcgc
     4801 gaatatttcc ccgatacctt ttcgactgcg gttcgacaaa aatctcgctg gatcatcggc
     4861 attgttttcc agggctttaa aacccataaa tggacctcca gcctgacgct gaactacttt
     4921 ctctggcgcg accgcaaagg ggcaatcagt aactttgtca gcttcctcgc gatgctggtg
     4981 atgatccagc ttttgctgtt gctggcgtat gaaagtttgt ggcccgatgc ctggcatttc
     5041 ctttctattt ttagcggcag cgcatggtta atgaccctgc tgtggctaaa ctttggcttg
     5101 atggttaacc gcatcgtgca gcgagtgatt ttcgtcactg gctactacgg cctcacgcag
     5161 ggcctgcttt ccgtcctgcg tcttttctgg ggcaacctga ttaactttat ggctaactgg
     5221 cgcgtgctga aacaggttct tcagcacggc gatccgcgtc gcgtcgcgtg ggataaaaca
     5281 acgcatgact tccccagcgt cactggcgat acccgctcgt tgcgcccgtt aggtcaaatt
     5341 ctgctggaaa atcaggtcat cactgaagaa caactcgata cagcactgcg taatcgcgtc
     5401 gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc tgattagcgc cgagcagctg
     5461 gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat ccatcgatgc ctggcagatc
     5521 ccgtcctcgc tgattgccga aatgccagcc tccgtagcgc tgcattatgc agtactgccg
     5581 ctgcgtctgg aaaatgatga gttaattgtc ggcagtgaag atggtattga cccggtttcg
     5641 ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt acgtcattgt tctgcgagga
     5701 caaattgtca cagggttacg ccactggtat gcacgccgac gcggtcacga tccgcgggca
     5761 atgttgtaca atgcggttca gcatcagtgg ctcacggaac agcagaccgg tgaaatctgg
     5821 cggcaatatg tgccgcatca gttcctgttc gccgaaatac tgaccacgct cggtcatatt
     5881 aatcgttcag caattaacgt gttgttattg cgccatgaac gcagttctct gccgctcggc
     5941 aagtttttgg tcaccgaagg cgttatcagc caggaaacgt tggatcgcgt cctgacaatt
     6001 caacgcgaat tacaagtttc gatgcaatca ctattactca aagcaggttt aaacacagaa
     6061 caggttgcgc aactggagtc cgaaaatgaa ggagaataac cttaatcgcg tcatcggatg
     6121 gtctggttta ctgctgacgt ctttattgag taccagcgca ctcgcagaca atatcggcac
     6181 cagcgcagaa gagctggggc tgagcgatta tcgccatttt gttatttatc cccgtctcga
     6241 taaggcgctg aaggcacaga aaaataacga cgaagcaacc gccatccgcg aatttgaata
     6301 tatacaccaa caggtgccgg ataatattcc gctgacttta taccttgcgg aagcctatcg
     6361 tcattttggt catgatgacc gggcgcggct gttgcttgag gatcaactga aacgtcaccc
     6421 aggagatgcc cgacttgagc gcagtctggc ggctattcca gttgaagtga aaagcgttac
     6481 gactgttgaa gaactgcttg cccagcaaaa agcgggcgac gctgcgccga ccctgcgttg
     6541 tcgcagtgaa gtcgggcaga atgccctgcg gctggcacaa ttacctgtcg ccagagcgca
     6601 actgaacgat gcgacgtttg ctgcatcgcc ggaaggaaaa acgctgcgaa ccgatctgct
     6661 gcaacgggca atctacctga aacaatgatc ccaggcagat acgctataca atgaagcacg
     6721 ccagcagaac acattaagcg cggcagaacg ccgtcagtgg tttgacgtgc ttcttgccgg
     6781 gcagctggac gatcggatcc tggcactgca atcacagggg atcttcaccg atcctcagtc
     6841 atatattact tacgcgaccg cgctggctta tcgtggcgaa aaagcacgcc tccagcatta
     6901 tctcattgaa aataagccgc tgtttaccac gggcgcacaa gagaaaagtt ggctctatct
     6961 gttatctaaa tacagcgcca accccgttca ggcattggcg aattatacgg tgcagtttgc
     7021 cgataaccgc cagtatgttg ttggcgcgac gctaccggtg ctgttaaaag aaggtcagta
     7081 cgacgcagcg caaaaactgc tcgccaccct ccccgccaat gaaatgcttg aggagcgtta
     7141 tgctgtcagc gtggcgaccc gtaacaaggc tgaatctctg cgtctggcac gattgctgta
     7201 tcagcaagaa ccgacaaatc ttccccgcct ggatcaactg acctggcaac tgatgcagaa
     7261 cgagcagtca cgcgaagctg ccgatttatt gctgcaacgc tatcctttcc agggcgatgc
     7321 gcgtgtcagc cagactttaa tggcgcgact ggcgtctctg ctggaaagtc atccttacct
     7381 agcaacgccg gcgaaggtgg cgattttatc gaaaccctta ccgctggcgg agcaacgtca
     7441 gtggcaaagt cagttgccgg gtattgcaga taattgcccg gcaatagttc gcttgctggg
     7501 cgatatgtcg ccttcctacg atgctgccgc ctggaaccgt ctggcaaagt gttatcggga
     7561 cacgctaccc ggtgtggcgt tgtatgcatg gcttcaggcc gaacaacgac aaccgagcgc
     7621 ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt gaggactacg ccaccgcact
     7681 ggcggcctgg cagaaaatca gtcttcacga catgagcaat gatgatctgc tcgccgccgc
     7741 caacaccgct caggcagcgg gaaatggcgc ggctcgcgat cgctggcttc aacaggcaga
     7801 acaacgtgag ctgggaaaca atgctcaata ctggtggctg catgcgcgac gttatatccc
     7861 agctcagcca gaactcgcac tgaacgatct cacgcgctca atcaatattg ccccttctgc
     7921 caactcttac gttgcgcggg cgacaattta tcgccaacgt cataatgttc cggccgcggt
     7981 gagtgatttg cgcgccgcgc tggaactgga accaaataat agcaacaccc aggcagcgct
     8041 tggttacgcc ttgtgggata gcggtgatat cgcacagtcg cgggaaatgc tcgaacaggc
     8101 gcataaaggg cttccggacg atccggcact gatccgacaa ctggcctacg tgaaccagcg
     8161 tctggatgac atgcctgcga cgcagcacta cgcccggctc gtgattgatg acattgataa
     8221 tcaggcataa ccccactgac cccagagcaa aatcagcaac gcttcaattt ccgccgtttg
     8281 catgaggagg tcggtcgccg ctggacgttc agtttcgatt cttccatcgg cttgcgttcc
     8341 ggcgcaatga gtaccgctaa caataatgtc ggcggtgcag cgcctgggaa aagctatcgt
     8401 agctacggac aactggaagc cgagtaccgc atcggacgca atatgctgct ggaaggcgac
     8461 ctgctctcag tttatagccg cgtctttgcc gataccggag aaaacggggt gatgatgccg
     8521 gtgaaaaatc cgatgtccgg caccggtctg cgctggaagc cgctgcgcga tcagatcttt
     8581 ttcctcgccg tcgaacagca gttgccgctg aacgggcaaa atggcgcatc cgataccatg
     8641 ctgcgcgcca gcgcctcatt ctttaatggc ggcaaataca gcgacgaatg gcacccgaac
     8701 ggttcaggct ggtttgccca aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat
     8761 attcaggcgt ggacggcaga ttatcgcgtc agctggcatc agaaggtagc taacggacag
     8821 actattgagc cttacgctca cgttcaggac aacggctatc gtgataaagg cactcagggc
     8881 gcgcagcttg gcggtgtcgg ggtccgctgg aatatttgga ccggcgagac gcactacgac
     8941 gcctggccgc acaaagtcag tctcggcgtc gagtatcaac atacctttaa ggcgattaat
     9001 caacgtaacg gagagcgcaa caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt
     9061 aagttcattt tcgtattgct gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa
     9121 ggtattatct ggcaaccaca aaaccgagat agtcaggtta ccgataccca gtggcagggg
     9181 ctgatgagtc agttacgttt tcaaggcttc gataccctgg ttttgcaatg gacccgttac
     9241 ggcgatgcat ttacccagcc agaacagcgc acgttattgt ttaagcgtgc cgcagctgcg
     9301 caacagactg gcctgaagct gattgtcggg ctgaacgccg atccggaatt ttttatgcac
     9361 cagaaacagt cgtctgcagc gctggaaagc tatcttaatc gcctgctggc tgccgatctc
     9421 cagcaagcca gattatggag cgccgcgcct ggcataacgc cggatggctg gtacatcagc
     9481 gcggaaattg acgacctgaa ctggcgcagc gaagccgccc gtcaaccttt gctaacatgg
     9541 ttaaacaacg cgcagcggct gattagcgat gtttcagcaa aaccggttta tatcagtagt
     9601 tttttcgccg gaaacatgtc gcccgatggc tatcgccaac tgctggaaca agttaaagca
     9661 accggagtta atgtctgggt acaggatggc agcggcgtgg ataaacttac cgctgaacag
     9721 cgtgaacgtt atctacaggc cagcgccgat tgccaaagct ccgccccagc aagcggcatt
     9781 gtttatgaac tgtttgtcgc cggcaaaggc aaaaccttta cagcgaaacc gaaaccggac
     9841 gcagaaattg cctcgctgtt agcgaaacgt tcctcttgcg gtaaagacat gctctatttc
     9901 tctctgcgct atttgcccgt cgcgcacggc attctcgagt attaaatccc ctccaggtaa
     9961 gtcgggtacg acctggctta cctctttcgc tctcgtcaga attccatcaa gattaattgc
    10021 taaaaagcat gctaaagaaa gaagatcgtt atcggcgaac gggccgatca ctcatgcagg
    10081 aagcatagat cctttgagcg gagtttctga tatctgagga gtgcgaaatg caattgagca
    10141 gcagtgaacc ttgcgtggtg atcctgaccg aaaaagaggt agaggtaagc gtcaataacc
    10201 atgctacgtt tacccttccg aaaaactacc tggccgcctt cgcgtgcaac aataacgtca
    10261 ttgaactctc aacgttaaat cacgtattaa tcacccacat caaccgtaac atcatcaacg
    10321 attatctgtt gtttttaaat aagaaattaa cctgtgtaaa gccctggtcg cggctggcaa
    10381 ccccggttat cgcttgtcat agccgtacac cggaagtgtt ccggctagcc accaaccaca
    10441 gcaagcagca atccagcaaa ccctgcgaag cggagttgac gcgcgcatta ctttttaccg
    10501 tattgtctaa ctttcttgag caatcgcggt ttattgccct actgatgtat atcttacgca
    10561 gcagcgtccg cgacagcgtc tgccgcatta ttcaaagcga tattcagcat tactggaatc
    10621 tgcgaattgt cgccagttcg ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa
    10681 acgaaaatac cagctatagc cagattgtca cagagtgtcg tatgcgttac gccgtacaga
    10741 tgttattgat ggataacaaa aatatcactc aggtggcgca attatgtggc tatagcagca
    10801 cgtcgtactt tatctctgtt tttaaggcgt tttacagcct gacaccgttg aattatctcg
    10861 ccaaacagcg acaaaaagtg atgtggtgaa gggcaaagcg gaaacggata agacgggcat
    10921 aaatgaagaa gaaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagg
    10981 tcgttgctct atccaactga gctaagggcg cgttgatacc gcaatgcggt gtaatcgcgt
    11041 gaattatacg gtcaaccctt gctgagtcaa tggcttttga tctggttgct gaacaagtga
    11101 acgaccgcgt ctgattttct gatttatttc gctatagcgg caaacaaacg cacaccgctg
    11161 cgcgtctgaa tcaagaaaac ccgtattttc atgtatcaaa gtacaattta ccgccctaac
    11221 ggaaaattgt ccgctcctat gagactggta actatgaaac caacgtcggt gatcattatg
    11281 gatactcatc ctatcatcag aatgtctatt gaagttctgt tgcaaaaaaa cagtgaattg
    11341 cagattatcc tgaaaacgga tgattaccgc ataaccatcg attatctccg aacccgtcct
    11401 gttgatttaa tcattatgga tatagacttg cccggaacag acggttttac cttcct
//
LOCUS       DAJTRB010000120        11288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTRB010000120 DAJTRB010000000
VERSION     DAJTRB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.120"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO330_003545"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO330_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5884280.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO330_003546"
     tRNA            complement(621..695)
                     /locus_tag="OO330_003546"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO330_003547"
     CDS             complement(769..1701)
                     /locus_tag="OO330_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5884281.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO330_003548"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO330_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5884282.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGVNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO330_003549"
     CDS             complement(2932..3990)
                     /locus_tag="OO330_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884283.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO330_003550"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO330_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5884284.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO330_003551"
     CDS             6068..6352
                     /locus_tag="OO330_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5884285.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO330_003552"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO330_003552"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5884286.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO330_003553"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO330_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5884287.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO330_003554"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO330_003554"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5884288.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2827 a         2582 c         2778 g         3101 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctcaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaaggggtca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt agggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggcctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catattgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg atactactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caggcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgcacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct gcgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt ccttaagata acctttcttc tgtcaatata ttaattaaat ctacatttac
    11161 tctttagtaa aattaaattg gatcaggggt gtggaaacat cactatgcgg ccccacacca
    11221 atgaatacaa ttttctacga cctgttcttt ttgggtcgca tgccgtattc aatggctggg
    11281 tataaaca
//
LOCUS       DAJTRB010000121        10926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTRB010000121 DAJTRB010000000
VERSION     DAJTRB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.121"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            155..505
                     /locus_tag="OO330_003555"
     CDS             155..505
                     /locus_tag="OO330_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5884289.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            558..953
                     /locus_tag="OO330_003556"
     CDS             558..953
                     /locus_tag="OO330_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5884290.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            994..1737
                     /gene="cmoA"
                     /locus_tag="OO330_003557"
     CDS             994..1737
                     /gene="cmoA"
                     /locus_tag="OO330_003557"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5884291.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1734..2705
                     /gene="cmoB"
                     /locus_tag="OO330_003558"
     CDS             1734..2705
                     /gene="cmoB"
                     /locus_tag="OO330_003558"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5884292.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="OO330_003559"
     CDS             complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="OO330_003559"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5884293.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLSAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5324..6424)
                     /locus_tag="OO330_003560"
     CDS             complement(5324..6424)
                     /locus_tag="OO330_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5884294.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6812..7558)
                     /gene="cutC"
                     /locus_tag="OO330_003561"
     CDS             complement(6812..7558)
                     /gene="cutC"
                     /locus_tag="OO330_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5884295.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7572..8138)
                     /locus_tag="OO330_003562"
     CDS             complement(7572..8138)
                     /locus_tag="OO330_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5884296.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8354..10087
                     /gene="argS"
                     /locus_tag="OO330_003563"
     CDS             8354..10087
                     /gene="argS"
                     /locus_tag="OO330_003563"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5884297.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10264..10752
                     /locus_tag="OO330_003564"
     CDS             10264..10752
                     /locus_tag="OO330_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5884298.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2671 a         2789 c         2602 g         2864 t
ORIGIN      
        1 ataatgggct tttaatccta tcgtttattg cttgcatagt agaatctaac catccatcac
       61 tcatagctaa ccctcggaaa tcttacattt ctgtctatca taaccactag catcaccttt
      121 aggaaccatc tgaaacggga ggtgtattta gaccttgcta atcttagaca gccgcccggt
      181 tcatgcatca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
      241 tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga
      301 tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca
      361 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
      421 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
      481 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
      541 ttttaaaggg agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa
      601 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg
      661 gtggttttag cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc
      721 ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
      781 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
      841 gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg
      901 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
      961 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     1021 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtcgctga agtcttcccg
     1081 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
     1141 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     1201 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac
     1261 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     1321 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     1381 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa
     1441 atttatcaag gactgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
     1501 gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt
     1561 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
     1621 tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg
     1681 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     1741 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
     1801 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt
     1861 ccaacacggt ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg
     1921 taaccgccga aagcgaagag ccactgagca ccgggcaaat taaacgcatt gaaacgctta
     1981 tgcggaacct gatgccatgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     2041 ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg
     2101 gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg
     2161 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
     2221 cggtgcgtaa actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac
     2281 agcttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc
     2341 gttcgccgct ggagcatctc tggcagttga aagatcaact ggtgaatgag ggtgaactgg
     2401 tgctggaaac gctggttatt gatggcgatg agaacacggt gctggtacca ggcgatcgtt
     2461 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
     2521 agaagtgtgg ttttgtagat atccgcattg tggatgtgtg cgttaccact actgaagagc
     2581 agcgacgcac tgaatggatg gttaccgaat ctctgtccga tttcctcgac ccacatgatc
     2641 cgagtaagac agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc
     2701 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaccc
     2761 ttgctaattc aatctattgc agggactatg taggcctgat aagcatagcg catcaggcag
     2821 ttttacgttt gcataacctc agcgcccgtt tccgggcgct attcacttct tacgcctgaa
     2881 cagcaggctt atcaaatgcc gttaacttcg gtgcgttacc cgtatatttt tcgatataca
     2941 ccagcgcaga gttaccggca caggcatttg cccgctgcga gctggggata tccgccgtta
     3001 acacgttcgc actgccgttt ttacacaagc cgttttccag atctggccat gcaccttcat
     3061 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac
     3121 cacgtttgtt ccagacacgc accagatcgc cattcgcaat accaaatcga gcagcatctt
     3181 cggtgtgaat agtgatcggt tcacgatcgg caaccgcata ttttttacgc agttccgcat
     3241 agttaagctg actgtgtaaa cggtgtgccg gatgtgcgct cagaagctgc aactgcttct
     3301 cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct
     3361 tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca
     3421 gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct
     3481 cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag
     3541 tgacgcgttg cgcacgggcc cctttctgag cggcatcata gaaaaatttc agccacgcca
     3601 tttcatcttt accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat
     3661 cggcaaacac gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg
     3721 gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg
     3781 tggtgatcgg taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa
     3841 cgacgatcat ctccggtttc tgccaggctt tgatcagacg attagtatcc tggtgatggg
     3901 taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac
     3961 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     4021 tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca
     4081 tttccggcag cacgccgcca acacgcgtcg ggttgccacc attggagtaa tgataagaga
     4141 aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca
     4201 tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg
     4261 tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa
     4321 tttccgccgt ccagaccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt
     4381 caaactgcgg atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     4441 tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga
     4501 tccaggtggc attatcgcca aagaattcga tggtttcgga gcggatagga tcaatggcaa
     4561 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
     4621 tgctccaggc aatttttaag gtattcagcg ggttcattcc ccacagcacc acaacctggc
     4681 tgttttccag aatcagcggc caggaggcct gctgttcata cacctctaca gaaccgacca
     4741 catgcggcat gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac
     4801 cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
     4861 agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgct
     4921 catgttgctc atgaatcagt tttaatgcct gttcccagct cacctgtaca taggtatctt
     4981 cgccacgacc tttcgccggt tgcagtggat tgtcgagata gctttttctc accatcggat
     5041 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta
     5101 tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgctca
     5161 tcgctcccca acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga
     5221 gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt
     5281 cacgtcttgt taatgccata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
     5341 tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cggttcgttt tcgctcatgc
     5401 tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg
     5461 cggcaataga ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
     5521 atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac
     5581 gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc gccttgatac
     5641 cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt
     5701 cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa
     5761 cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga gaagttttga
     5821 aaggataaat atgtgcgcca ttagtcactg aagtagcggc ctgactttcc agctcatgcg
     5881 ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga
     5941 tacaggtttc gcctcccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg
     6001 aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag tcagttgctt
     6061 ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac ttatcgtcac
     6121 tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa
     6181 ataaataatc catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt
     6241 tctggttcga aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc
     6301 aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc
     6361 cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc
     6421 gtatagattc ctctaaataa attccagaac accgaaatca ttgtataatt aaatacacaa
     6481 cgaattaacc gcttgcataa ctcggcacaa cactgcctga aataatcgat aaagaatatg
     6541 atttattaca atgtaatcat taattgctaa ggaataaccc agttgctatt tagaatatat
     6601 atttatcagt tttagcaatt taaatcccat aatcaatgtg aatataaaaa atatttttag
     6661 cacaacgcta tgcgcactgt cacccgctca acagatcacg gttatcgttc gttttttata
     6721 ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat
     6781 catattgggc gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc
     6841 ctttcatttc agcaaccgcc gccccgtcta cgatataacg cgaatactcg tccgcgtgtt
     6901 catctgatga catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc
     6961 ccgcggagct atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac
     7021 ggactcctgc tccagccata atgattggag catcacgatg ggcaataagt tccataattt
     7081 ttgataaacc ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca
     7141 attccgcaag attattgagt gtatataaag ggttagcgca catatcgaag gcgcgatgaa
     7201 aagtcactgc cagcggaccg gcagcagcca ttattttttc cattcgtggc atatcgacat
     7261 tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa acctaattcg cggaccgtgc
     7321 gcacatcctc aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg
     7381 ggcgaattat cggatgcaca gggatcgtca cccgctggcg cacggatttc agtaccccca
     7441 acgacggcgt taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt
     7501 tttgctgcgc cgttagtgca cattccatgc tgtaacagca aatttccagt aatgccattt
     7561 ttgctcctta attacgctga ctgctcgctg gcaacgatct cttcaatgga ccacggatga
     7621 aacttaatag ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc
     7681 tcaccttttg gtgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa
     7741 agcaatgcca gcgcacgggc attcagcgtt tctggatcac ccggcagaac gatttcaata
     7801 tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc cacattggaa
     7861 aactgccagt gcgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca
     7921 ttgatcattt tttctgacaa aagctcgcca cactgttcaa aaccgcgacg ccagcgctca
     7981 gcggtgacgt tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc
     8041 aaccccagac ggcgggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca
     8101 atatcctgca gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta
     8161 aaagcgttaa tttaccctgt tgccctgtgc caaccaaccg ctgatttcac gccgcttctg
     8221 atgcaatagt gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc
     8281 agtatactcc cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat
     8341 gtaaggtatt ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat
     8401 tgcggcaggc gcgcctgcgg attgcgaacc gcagattcgt cagtcagcaa aagttcagtt
     8461 cggcgactat caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg
     8521 acaattagca gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga
     8581 gatcgccggt ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt
     8641 tcagcaggcg ctggcgtccg atcgtctcgg tgttgctacg ccagaaaaac agaccattgt
     8701 ggttgactac tctgcgccaa acgtggcgaa agagatgcac gtcggtcacc tgcgctctac
     8761 cattattggt gacacagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc
     8821 aaaccacgtc ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca
     8881 gcagcaggaa aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc
     8941 gaaaaagcat tacgacgaag atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa
     9001 actgcaaagc ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat
     9061 gacgcagaac cagatcactt acgatcgtct caacgtgacg ctgacccgtg atgacgtgat
     9121 gggcgaaagc ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg
     9181 tctggcagta gaaagcgaag gggcgaccgt cgtattcctt gatgagttta aaaacaagga
     9241 aggcgaaccg atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac
     9301 tgatatcgcc tgtgcgaaat atcgttatga aacactgcat gccgatcgcg tgctgtatta
     9361 catcgactcc cgtcagcatc aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg
     9421 ctatgtaccg gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga
     9481 cggcaaaccg ttcaaaaccc gcgcgggtgg taccgtgaaa ctggcagacc tgctggatga
     9541 agccctggag cgtgcacgtc gtctggtggc agaaaagaac ccggatatgc cagctgacga
     9601 gctggaaaaa ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa
     9661 aaaccgcacc acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac
     9721 cgcgccatac atgcagtatg cttacacgcg tgtattgtcc gtgttccgta aagcagaaat
     9781 taacgaagag caactggctg cagctccggt aatcatccgt gaagatcgtg aagcgcaact
     9841 ggcagctcgc ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc
     9901 gcatgtaatg tgtgcttacc tgtacgatct ggccggtctg ttctctggct tctacgagca
     9961 ctgcccgatc ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca
    10021 actgacagcg aagacgctga agctgggtct ggatacgctg ggtattgaga ctgtagagcg
    10081 tatgtaatcg atttttcgtg agagtgaagc ctgatcaggg ttagccgatc aggctttttt
    10141 attgccatct aaatgtattc tgaaaacgga catgccattg ttttctcact gttgagtaag
    10201 aggccagaag cgtaatatcc aggcccagga aaacgataac ggttcaattt aaggaatacc
    10261 gaagtgttta aatttcttgt attaacatta ggcattatct cttgccaggc ttacgcagaa
    10321 gatacggtta tagtaaacga ccatgacact tcagccatca aagattgttg gcaaaaaaat
    10381 tcagatgatg atactgacat taacgtgatc aaatcatgcc tgcgacaaga atacaatctc
    10441 gtcgatgcgc aattaaataa agcctatggt gaagcttatc gttatataga acaagtgcca
    10501 cgcacaggtg caaaaaaact tgatactgaa caacttaact tgcttaaaaa atcacagcga
    10561 gcctggctgg attttaggga caaagaatgt gaattaatcc tttcaaatga ggatgttcag
    10621 gatttaagtg acccctattc tgaatcagaa tggctatcat gtatgatcat acagaccaat
    10681 acgcgaactc gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg
    10741 acaagaggat aattcacatc tttttggcat gttttgttgc aagctattcc tgataaatga
    10801 ttgcaacaaa acatccagcc ttttgactga gttattaaac atactcgcga gcgcgtaatt
    10861 ttttgtcttt cagcgataat tcacaatcac ttcattccgt tgtaccttta gcgtggaatt
    10921 aaccgc
//
LOCUS       DAJTRB010000122        10888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTRB010000122 DAJTRB010000000
VERSION     DAJTRB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.122"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..241
                     /locus_tag="OO330_003565"
     CDS             <1..241
                     /locus_tag="OO330_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884299.1"
                     /translation="NGSVRYCQYTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAAL
                     ITYFAGNWHSIAIYLLAGCLISAMTALLMKDNQRS"
     gene            343..1797
                     /gene="amn"
                     /locus_tag="OO330_003566"
     CDS             343..1797
                     /gene="amn"
                     /locus_tag="OO330_003566"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5884300.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2140..2856
                     /locus_tag="OO330_003567"
     CDS             2140..2856
                     /locus_tag="OO330_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5884301.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3312..3387)
                     /locus_tag="OO330_003568"
     tRNA            complement(3312..3387)
                     /locus_tag="OO330_003568"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3352..3354),aa:Asn,seq:gtt)
     gene            complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="OO330_003569"
                     /pseudo
     CDS             complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="OO330_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5120..5195
                     /locus_tag="OO330_003570"
     tRNA            5120..5195
                     /locus_tag="OO330_003570"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5153..5155,aa:Asn,seq:gtt)
     gene            complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="OO330_003571"
     CDS             complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="OO330_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5884302.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6285..7202)
                     /gene="nac"
                     /locus_tag="OO330_003572"
     CDS             complement(6285..7202)
                     /gene="nac"
                     /locus_tag="OO330_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5884303.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7530..7605
                     /locus_tag="OO330_003573"
     tRNA            7530..7605
                     /locus_tag="OO330_003573"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7563..7565,aa:Asn,seq:gtt)
     gene            complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="OO330_003574"
     CDS             complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="OO330_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5884304.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="OO330_003575"
     CDS             complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="OO330_003575"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5884305.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="OO330_003576"
     CDS             complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="OO330_003576"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5884306.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10489..>10888)
                     /locus_tag="OO330_003577"
     CDS             complement(10489..>10888)
                     /locus_tag="OO330_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5884307.1"
                     /translation="SRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFD
                     YGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHF
                     RDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3025 a         2773 c         2356 g         2734 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaatgttt ggtgccagtt atcgctacag
       61 tggtgctgga gtcggttatc aggttgccag tgtggttggc ggtggattta caccttttat
      121 tgccgctgca ctcatcactt actttgccgg gaactggcat agcatcgcca tttatttgct
      181 ggctggatgc ctgatttctg caatgaccgc tttattgatg aaagacaatc aacgctcgtg
      241 atagcctggc gaagatcatc cgatcttcgc cttatacttt tgtttcacat tcctgtgaca
      301 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
      361 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtt
      421 gctttacgca acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc
      481 cgtaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca
      541 aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg
      601 attactcgcc ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag
      661 gattatggtg cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc
      721 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc
      781 ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg
      841 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
      901 cgccattata ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac
      961 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
     1021 ccctacattg ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca
     1081 gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt
     1141 accgctgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa
     1201 accatctgcg atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt
     1261 ggcggattac gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc
     1321 gatgaccacg ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa
     1381 gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc
     1441 aaacagcggc tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt
     1501 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
     1561 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
     1621 tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa
     1681 ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
     1741 ggcgaccgac tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa
     1801 gttatgaacc ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag
     1861 cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta
     1921 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc
     1981 aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc
     2041 taatagacga gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt
     2101 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
     2161 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
     2221 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
     2281 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
     2341 attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt
     2401 ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt
     2461 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
     2521 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
     2581 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
     2641 attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct
     2701 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
     2761 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
     2821 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt
     2881 tatggcagat ttctttaatc tcatataatt cttataaaaa atataatatt taactcgtca
     2941 tattgcttat aaaaaatccc cgtccccaga gaaataatat ttattaaaat ggcaggtctt
     3001 ctttttctga ttaccgaaga caaagtggca attacgtata gtttcccgat aaagacgcga
     3061 cagctacgtc ccgcataagg catttttctc tttatctttg tacggtactt catggaacag
     3121 aatttttgac cttgtgaatc gtgatatctg ttggggaggg gcaatttgct cactgaagcg
     3181 tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggccact tttttacaaa
     3241 gtggccgggt aaaaataatt tttatttttt aactgtcctg agtctcatag agatgtctca
     3301 aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg
     3361 ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt agcgatgccc
     3421 acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat aatctgcatt
     3481 ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggctcg gatcgagggt
     3541 atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc cgcacagccc
     3601 agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg attccctgca
     3661 cataaccgac tacaacccgg caaccccaca tgctcaacat agaaacccac atggcgtaac
     3721 gggcatcacg agcaccctta aatccagcgg gtagcaccca tgaggcggac caaataggca
     3781 taaataaagc atttagctaa atcagaatca caacgacgtg tttaacctgt ggatcctggg
     3841 tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg gccgttaatc
     3901 caagagtgga cagccagaac acatgccgca actgaatctc tgcttgcgct atctgcccta
     3961 cccccaacct tcggcctgta atgatcgtag aagcagaacc gagcgcactt ccgggtaagt
     4021 tgataagagc cgcaattgag aacgcgataa aatttccggc aataacgctg gtccccatcc
     4081 cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg
     4141 ctggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc
     4201 tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa
     4261 ctgcgccaat ataacgagaa atggttaaac ccaacccagc cccgaccagg agaaaaggcc
     4321 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     4381 cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag tgatggcagc
     4441 ggctggataa ctgagtacgg tcagctccag ataagtcaac gccagtgctt taacttctgt
     4501 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa ttagcgttgc
     4561 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     4621 tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca gtagtaccaa gatcgatagc
     4681 tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc cggccatcgc
     4741 atcttttccc agccaactga ccagaaaagt gctcaggacc cccatcaaca ggacacaggc
     4801 gttctccatg aagataggaa cagcaagcgg ggttatctcg cgctagaaca acactttgta
     4861 gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     4921 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg agggagaatc
     4981 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     5041 aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt tcgccgttaa
     5101 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     5161 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     5221 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctggcg
     5281 cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc aaacatagtt
     5341 acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg
     5401 cgtatccagg cggattaaat tctcttcctc ttgttcgccg ctggattgtt cggcaactaa
     5461 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gtgcacttaa
     5521 tacaatatct gccggcaaac ctttgcgggt aaatgcgtca tcagtgcgtg agcgccccgt
     5581 aattccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     5641 aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccaac aaaaccacgg
     5701 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     5761 gccattttgc aacaacgtcg caatttcctg tggcgaaccc tggattagct cgagccgaac
     5821 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     5881 atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt ctgcaagccg
     5941 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     6001 ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caaattcatc
     6061 ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca tattcgcaac
     6121 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     6181 cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat gcccggcata
     6241 acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc
     6301 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     6361 tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt
     6421 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc
     6481 gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     6541 gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca
     6601 cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta
     6661 acatcaacgt tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc
     6721 aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc catatcgagt
     6781 tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg
     6841 acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     6901 gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc
     6961 gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata gagaattttt
     7021 ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca
     7081 ccttccagta tggcaacctg ctggctgagc gctggttgtg cgatatgcaa tacttcagca
     7141 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     7201 atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca accagtatgc
     7261 aagatgcctg ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaga
     7321 gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc tgccctataa
     7381 aaggagggta tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt
     7441 tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc ctttgacatc accatgcact
     7501 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     7561 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     7621 gctttcgagc aggcttttta ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     7681 cagaactaat ttgcacaggc attcccgatc gatgttgcaa cgcagcattt gcgcgattta
     7741 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     7801 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact
     7861 ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc
     7921 ctacaggaac attatcaaac agatatttga tatcgtcatt acgcagacga atacagccct
     7981 gacttacacg gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     8041 caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca
     8101 aactctcccc tcgtttcgca tattcgcgcc gggtgttcgg cgttggcgtc caggttggtg
     8161 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     8221 cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa taaagacgca
     8281 tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     8341 gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca
     8401 gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt
     8461 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     8521 gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaagaatat
     8581 ttacacgacg catcatgtcc ctttcctaag tcgcgaatac tatccgataa gtatagcttt
     8641 tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg tagaacaata
     8701 ttactggcag caagttcgcc catgttgttg tatatcgcac aagcagcttc gatgatggac
     8761 atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag ataaggctcc
     8821 agccccaaat gcgagagcgc tatacgcgcg cctttttctg ccgacaagtg agaaggaatg
     8881 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga
     8941 aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac tccagctatt
     9001 ccgaccaaat caaatccacc cacttttgcc aggacatcaa caccatcctg aggatttggt
     9061 tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt atctgtcggc
     9121 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     9181 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg
     9241 tttttcgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     9301 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     9361 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac gttagcgccc
     9421 gcttgtgctg ccagcacaca cacgccggtt gttccacggg tcatattttc agcctgtatg
     9481 gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     9541 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcacccccgg cattcctgcc
     9601 agttgtatgg caagcgcctc cagctttcct aggctaccaa caggtttgaa taacccgtca
     9661 acatgccgtt gtgcacgcga catagcggca gaattgatgg ctgggatcgt attcagtaaa
     9721 tcggcaatta tttgcatgtc acgtttcttt atagcagtgc cagcaggaag accagttcac
     9781 caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta cgtttgagaa
     9841 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     9901 ccagcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     9961 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat
    10021 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
    10081 tcggttcgcc acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa
    10141 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
    10201 cgcgactacg tgcagaaaat acgccatcac aggtatcagc gaggccgtcc agatggaatt
    10261 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt gcgccacacc
    10321 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaatcaaag
    10381 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
    10441 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttgctc atttgatttt
    10501 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt
    10561 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
    10621 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
    10681 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
    10741 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attccagcaa
    10801 cacagcctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
    10861 cactgtgcgc cagtgctccg ggcggctt
//
LOCUS       DAJTRB010000123        10842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTRB010000123 DAJTRB010000000
VERSION     DAJTRB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.123"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            51..1541
                     /locus_tag="OO330_003578"
     CDS             51..1541
                     /locus_tag="OO330_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5884308.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTFDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="OO330_003579"
     CDS             1593..2750
                     /locus_tag="OO330_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5884309.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="OO330_003580"
     CDS             complement(2716..2838)
                     /locus_tag="OO330_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884310.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="OO330_003581"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="OO330_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="OO330_003582"
     CDS             4764..7124
                     /locus_tag="OO330_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5884311.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..9964
                     /locus_tag="OO330_003583"
     CDS             7181..9964
                     /locus_tag="OO330_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5884312.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSHLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10325..>10842
                     /locus_tag="OO330_003584"
     CDS             10325..>10842
                     /locus_tag="OO330_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5884313.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARY"
BASE COUNT         3198 a         2401 c         2364 g         2879 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttaccttc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7381 agaagacaat gagcgcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7441 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7501 cgatgttaat gcctatacca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7561 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7621 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7681 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7741 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7801 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7861 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     7921 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     7981 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8041 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8101 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8161 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8221 tgcacgagac gataatatgc aggacgctgc gaatgcatta atggcagagt tggcaaccat
     8281 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8341 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcacct
     8401 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8461 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8521 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8581 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8641 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8701 tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8761 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8821 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     8881 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     8941 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9001 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9061 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9121 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9181 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9241 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9301 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9361 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9421 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9481 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9541 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9601 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9661 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9721 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9781 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9841 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
     9901 tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa
     9961 ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta
    10021 tgtaataatt ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat
    10081 gcctgatttt tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa
    10141 acaccttgcc accacccaag aattctctat tacattgttt ttaatgaaaa aataccattc
    10201 caacaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
    10261 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
    10321 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
    10381 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
    10441 cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
    10501 cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc
    10561 cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
    10621 ttgcgtaaac atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt
    10681 tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg cgatgaaatg
    10741 gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa acctggtgtg
    10801 cggtccggtg caaatctgct ggcataaatt cgcccgctac tg
//
LOCUS       DAJTRB010000124        10725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTRB010000124 DAJTRB010000000
VERSION     DAJTRB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.124"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..481)
                     /gene="fimA"
                     /locus_tag="OO330_003585"
     CDS             complement(<1..481)
                     /gene="fimA"
                     /locus_tag="OO330_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5884314.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     "
     gene            962..1828
                     /gene="folD"
                     /locus_tag="OO330_003586"
     CDS             962..1828
                     /gene="folD"
                     /locus_tag="OO330_003586"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5884315.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1830..2042
                     /gene="ybcJ"
                     /locus_tag="OO330_003587"
     CDS             1830..2042
                     /gene="ybcJ"
                     /locus_tag="OO330_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5884316.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            2150..2659
                     /locus_tag="OO330_003588"
     CDS             2150..2659
                     /locus_tag="OO330_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5884317.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(2695..4080)
                     /gene="cysS"
                     /locus_tag="OO330_003589"
     CDS             complement(2695..4080)
                     /gene="cysS"
                     /locus_tag="OO330_003589"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5884318.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            4254..4748
                     /gene="ppiB"
                     /locus_tag="OO330_003590"
     CDS             4254..4748
                     /gene="ppiB"
                     /locus_tag="OO330_003590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5884319.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            4751..5473
                     /gene="lpxH"
                     /locus_tag="OO330_003591"
     CDS             4751..5473
                     /gene="lpxH"
                     /locus_tag="OO330_003591"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5884320.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5591..6100
                     /gene="purE"
                     /locus_tag="OO330_003592"
     CDS             5591..6100
                     /gene="purE"
                     /locus_tag="OO330_003592"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5884321.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            6097..7164
                     /gene="purK"
                     /locus_tag="OO330_003593"
     CDS             6097..7164
                     /gene="purK"
                     /locus_tag="OO330_003593"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5884322.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(7301..8194)
                     /locus_tag="OO330_003594"
     CDS             complement(7301..8194)
                     /locus_tag="OO330_003594"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5884323.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPKQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(8191..8838)
                     /locus_tag="OO330_003595"
                     /pseudo
     CDS             complement(8191..8838)
                     /locus_tag="OO330_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            8841..10073
                     /gene="allC"
                     /locus_tag="OO330_003596"
     CDS             8841..10073
                     /gene="allC"
                     /locus_tag="OO330_003596"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5884324.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            10084..>10725
                     /gene="allE"
                     /locus_tag="OO330_003597"
     CDS             10084..>10725
                     /gene="allE"
                     /locus_tag="OO330_003597"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5884325.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT         2537 a         2842 c         2803 g         2543 t
ORIGIN      
        1 cggtgccttt ataacgtgca gaaaaatgaa gaacgttggt cccggggatc aagttctggt
       61 tggttgagaa gctattccca tcaggtttga gaattgcgga tgtattatca agtatttcaa
      121 taccgacacc cgttgctgtt gttgtatttg tactggatgc aatggccaat aaattatcat
      181 tgattgcatc agcctgacca gaaaatgcaa cggcagcatt agcggcaaca acaggatcgc
      241 agtcgttcaa ctgaatggtg aatggaatta atgcagaggt attaccaaca gcattgaaaa
      301 tatcggtacg atattgaccg agtgtgacaa cctggtctgc cgagtcagta ttcactgaac
      361 aggcagcatt caccagttcg ccttcaaagt gaattgtacc gccatctaca acggcagcat
      421 aactacccgt agcggcgaat agtgcggcag ccagcgcaga cgaaataaat cttaatttca
      481 tatatattcc ttcaatctca tttatcgact ccacatccgt atataaccga ttactttatt
      541 taagacactg atagtagtaa aattcctttt tatcctctaa gaatgtctta attgaaaata
      601 tgcactctat tctaaaaaat agagagcccc gttagatgaa tacttccgcg caaaatatat
      661 tcaacacaaa tatagacctg aagcggtaaa ttaccaggct gaaaattctt tttatattgt
      721 caggtatttc ttaaattatc ttaatcctta aacaaggaaa taaatcagtt ccagatttac
      781 aacgccatca tgtacgaaaa atgaagcttt caatctcagc gacctcagcg acggtgcgcc
      841 taaccttcgc aagaggtcgc ttcacgcgat aaatctgaaa cgaaacctga cagcgcgccc
      901 cgcttctgac aaaatagacg catccccttc gacctacgta acagatggaa tcctctctct
      961 gatggcagca aagattattg acggtaaaac gattgcgcag caggtgcgct ctgaagttgc
     1021 tcaaaaagtt caggctcgta ttgcagccgg actgcgggca ccaggactgg ccgttgtgct
     1081 ggtgggtagt aaccctgcat cgcaaattta tgtcgcaagc aaacgcaagg cttgtgaaga
     1141 agtcgggttc gtctcccgct cttatgacct cccggaaacc accagcgaag cggagctgct
     1201 ggagcttatc gatgcgctga atgccgacaa caccatcgat ggcattctgg ttcaactgcc
     1261 gttaccggcg ggtattgata acgtcaaagt gctggaacgt attcatccgg acaaagacgt
     1321 ggacggtttc catccttaca acgtcggtcg tctgtgccag cgcgcgccgc gtctgcgtcc
     1381 ctgcaccccg cgcggtatcg tcacgctgct tgagcgttac aacattgata ccttcggcct
     1441 caacgccgtg gtgattggcg catcgaatat cgtcggtcgc ccaatgagca tggaactgtt
     1501 gctcgcaggt tgcaccacca ccgtgactca ccgcttcact aaaaatctgc gtcatcacgt
     1561 agaaaatgcc gatctgttga tcgttgccgt tggcaagcca ggctttattc ccggtgactg
     1621 gatcaaagaa ggcgcaattg tgattgatgt cggcatcaac cgtctggaaa atggcaaagt
     1681 tgtgggcgac gtcgtgtttg aagacgcggc taaacgcgcc tcatacatta cgcctgttcc
     1741 cggcggcgtt ggcccgatga cggttgccac gctgattgaa aacacgctac aggcgtgcgt
     1801 tgaatatcat gatccacagg gtgagtaaca tggcgacatt ttctttaggt aaacatccgc
     1861 acgttgagct gtgcgacttg ctgaaactgg aaggctggag cgaaagcggc gcgcaggcga
     1921 aaatcgcgat tgccgaaggc ctggtgaaag tcgacggtgc ggttgaaacg cgcaaacgct
     1981 gcaaaatcgt cgccggtcag acagtgagtt ttgcaggaca cagcgtacag gttgttgcct
     2041 gatccctttc ccggcctgta tcaacaggcc ggttctcttt tcagactttt gccctctacg
     2101 ccccgttcat ccatgctgat agcatcgaca ttttgccgaa gcgaccatca tgccaaccgt
     2161 tattacgcac gccgccgttc ccctttgcat tggtttaggg ttgggttcga aaggtctgtt
     2221 atttgccgga atcatcctgg cgatgctgcc cgatgccgac gtattgtcgt ttaaatttgg
     2281 cgttgcttac ggcaatgttt ttggtcatcg cggttttacc cattcgctgg tgtttgcgtt
     2341 tgttgtcccg ctcttatgtg tgctgattgg acgtcgatgg ttcagggctg ggctgattcg
     2401 ctgctggctg tttttaaccg tctcgttgct atcgcacagc ttactggatt cggtaaccac
     2461 tggcggtaag ggcgttggct ggctgtggcc gtggtcagat gaacgctttt tcgctccctg
     2521 gcaggtgatt aaagtcgcgc cgtttgcatt gtctcgttac accacagcgt acgggcatca
     2581 ggtgattatt tccgaattga tgtgggtgtg gttgccgggg atgctactga tgggaatgtt
     2641 gtggtggcgc agacgataac cggatgcgaa aactcgcatc cggcaattgc gcaattactt
     2701 acgacgccag gtggtccctt gcgggccatc ttccagcacg atccccatct cgttaagacg
     2761 gtcacgtgcc gcatctgccg ccgcccagtc tttcgcttta cgggcatcca gacgctgttg
     2821 aattaacgct tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc tttgcaggaa
     2881 cgcttccggt tcttgctcca gcaggcccaa tacagcggaa agtttacgca ggtgagatgc
     2941 cattgcattc gccgctgcca tatcttctgc tttcagacgg ttcacttcac gcgccatatc
     3001 aaacagcacg gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt caataaagcg
     3061 cgcttcaaac gcttcgccac cggcaggcgc aacggtttta tctgtgccgc gcagcgcagt
     3121 gtagaggcgc tccagcgccg cacgcgcctg cttcaggttc tcttcgctgt agttcagctg
     3181 gctgcgatag tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt agtatttcag
     3241 cacatcgcgc acggtaaaga agttacccag cgatttggac atcttctcgc ggtcaaccat
     3301 caccattccg gagtgcatcc agtagttcac atactgacca tcatgggcac aggtggactg
     3361 cgcgatttcg ttctcgtggt gcgggaacat caggtctgaa ccgccgccgt ggatatcaaa
     3421 gtggttaccc agctgcttgc agttcatggc cgaacattca atgtgccagc caggacgacc
     3481 cgcgccccac ggagacggcc agctcggttc gccctctttc gacatcttcc acagaacgaa
     3541 gtccatcggg ttgcgtttgt catcaaccac gtcaacgcgc gcgccagcct gcagctggtc
     3601 gagatcctga cgcgacagca cgccataagt tggatcggtc gggacgtcga acatcacgtc
     3661 gccgttgtcc gccacataag cgtgaccttt ggcgatcagt tgttcagtga gttcaataat
     3721 ttctgcgata tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt tcaaagcatc
     3781 aaaatctttg tgcatttcgg cgatcatgcg atccaccagc gccacaaagc tttcgccatt
     3841 ttcattggcg cgtttgatga ttttgtcgtc gatatcggta atgttgcgca catacttcag
     3901 tttatagccg aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa aggtacgccc
     3961 gtgaccgata tgacagagat cgtaaacggt gattccacac acgtacatgc cgacttcccc
     4021 ggcgtgaata ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga tttttagcat
     4081 cgaagattcc gtttagacat gtgtgggtaa ttgagttgcg tataataccc atatttcccg
     4141 cgcgaatcag catacattgc gtgatgatcc gatcgtgcgg ttatgctata acaccaccct
     4201 atatatgacc cgaactgggt tgaagcacca atcaaacgga acaggatgca aaaatggtta
     4261 ctttccacac caatcacggc gatattgtca tcaaaacttt tgacgataaa gcacctgaaa
     4321 cagttaaaaa cttcctggac tactgccgcg aaggttttta caacaacacc attttccacc
     4381 gtgttatcaa cggctttatg attcagggcg gcggttttga accgggcatg aaacaaaaag
     4441 ccaccaaaga accgatcaaa aacgaagcca acaacggcct gaaaaatacc cgtggtacgc
     4501 tggcaatggc acgtactcag gctccgcact ctgcaactgc acagttcttc atcaacgtgg
     4561 ttgataacga cttcctgaac ttctctggcg aaagcctgca aggttggggc tactgcgtgt
     4621 ttgctgaagt ggttgacggc atggacgtgg tagataaaat caaaggtgtt gcaaccggtc
     4681 gtaacggtat gcaccaggac gtgccaaaag aagacgttat cattgaaagc gtgaccgtta
     4741 gcgagtaatc gtggcgacac tctttattgc agatcttcat ctctgcgtgg aagaaccggc
     4801 gatcaccgcc ggttttctgc gttttttagc gggggaagcc cgcaaggccg acgcgctgta
     4861 tattcttggc gatctgtttg aagcatggat tggcgacgac gatcccaacc cactccatcg
     4921 ccagatggcg gcagcgatca aagcggtgtc cgattccggc gttccctgtt atttcattca
     4981 tggcaaccgt gattttctgc tcggcaaacg ctttgcccgt gaaagcggca tgacattatt
     5041 gccggaagaa aaggtgctcg aactttatgg tcgccgggtg ttgattatgc atggcgacac
     5101 gctgtgcacc gatgacgcgg gttatcaggc ttttcgcgcc aaagtccaca aaccctggct
     5161 gcaaatgcta ttcctcgccc tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg
     5221 cgcgaacagc aaagaagcca acagcagtaa atcgctggcg atcatggacg ttaaccaaaa
     5281 cgctgtggtc agtgcgatgg aaaaacatca agtgcaatgg ctaatccacg ggcataccca
     5341 tcgcccggcg gtgcatgaac ttatcgccaa ccagcaacct gcttttcgcg tggtactggg
     5401 tgcctggcat acggaaggtt cgatggtgaa agtcacggct gatgacgttg agctgattca
     5461 ttttccgttt taaaaaaccc acaactttgc tgatttcaca gccacgcaac cgttttcctt
     5521 gctctctttc cgtgctattc tctgtgccct ctaaagccga gagttgtgca ccacaggagt
     5581 tttaagacgc atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga tggggtccaa
     5641 aagcgactgg gctaccatgc agttcgccgt cgaaatcttc gaaatcctga atgtcccgca
     5701 ccacgttgaa gtggtttctg ctcaccgcac ccccgataaa ctgttcagct tcgccgaaag
     5761 cgccgaagag aacggttatc aggtgattat tgcgggcgca ggcggcgcag cgcacctgcc
     5821 aggcatgatt gccgccaaaa cgctggtgcc ggtgctgggc gtgccagtac agagcgccgc
     5881 actgagcggt gtcgatagcc tctactccat cgtacaaatg ccgcgcggca ttccggtggg
     5941 tacgctggcg attggtaaag ctggcgcggc aaacgcggca ttactggcag cacaaattct
     6001 cgcgactcac gataaagagc tacaccagcg tctgaatggc tggcgcaaag cccagaccga
     6061 cgaagtgctg gaaaacccgg acccgcgagg tgcggcatga aacaggtttg cgtcctcggt
     6121 aacgggcagt taggccgtat gctgcgtcag gcaggcgaac cgttaggcat tgctgtctgg
     6181 ccggtcgggc tggacgctga accggcggcg gtgccttttc aacaaagcgt gattaccgct
     6241 gagatcgaac gctggccgga aaccgcatta acccgcgagc tggcgcgtca tccggccttt
     6301 gtgaaccgcg atgtgttccc gattattgct gaccgtctga ctcagaaaca gcttttcgat
     6361 aagctccacc tgccgaccgc gccgtggcag ttacttgccg atcgcagcga gtggcctgcg
     6421 gtgtttgatc gtttaggtga actggcgatt gttaagcgtc gcactggtgg ctatgacggt
     6481 cgcggtcaat ggagtttacg cgccaatgaa accgaacagt taccggcaga gtgttacggt
     6541 gaatgtattg tcgagcaggg cattaacttc tctggtgaag tatcgctggt tggcgcgcgc
     6601 ggctttgatg gcagcaccgt gttttatccg ctgacgcata acctgcatca ggacggtatt
     6661 ttgcgcacca gcgtcgcttt tccgcaggcc aacgcacagc agcaggcgca agccgaagag
     6721 atgctgtcgg cgattatgca ggagctgggc tatgtgggcg tgatggcgat ggagtgtttt
     6781 gtcaccccgc aaggtctgct gatcaacgaa ctggctccgc gtgtgcataa cagcggtcac
     6841 tggacacaaa acggtgccag catcagccag tttgagctgc atctgcgggc gattaccgat
     6901 ctgccgttac cgcaaccagt agtgaataat ccgtcggtga tgatcaatct gattggtagc
     6961 gatgtgaatt atgactggct gaaattgccg ctggtgcatc tgcactggta cgacaaagaa
     7021 gtccgtccgg ggcgtaaagt ggggcatctg aatttgaccg acagcgacac atcgcgtctg
     7081 actgcgacgc tggaagcctt aatcccgctg ctgccgccag agtatgccag cggcgtgatt
     7141 tgggcgcaga gtaagttcag ttaagcaggt gctcatttga ttgccggatg cggcgtaaac
     7201 gccttatccg gcttaccgct ccggtaccca ttgtaggcct gataagacgc gtcaagcgtc
     7261 gcatcaggca tggtgcaccg attgccggat gctctcatca ctacagcgaa atacaggtcc
     7321 ccgcttcgcc cgccagcgtc tcttcaattc gcgataacgc cccaatccac gcgggtttac
     7381 tacggcttct gacataacca ctcaccgccg ttaccttcgg ccccatcgaa ccatcggctt
     7441 tggcaaatgg cgctaactca tccggtgtgg catggcgaat ggcacgttgc ttcggcgttc
     7501 cccagttttc atataccgca tcagcatcgg tgaggatcac cagtccatcg gcattaatct
     7561 gctcggcgag caacgcagcg gcgagatctt tatcaatcac cgcttcactc cctgcaccat
     7621 cttccgtcac aggcacaccg ccgccgccac tgcaaatcac cacatgcccc tctttgagca
     7681 acaactcgat ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccggc
     7741 gcaaatattt accatcacgt ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt
     7801 cttctggctg ataaaccgga ccaataaatt tctctggctg caaaaacgcc ggatcatcag
     7861 gcgaaacctc aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc tgtgcgctca
     7921 agctctgcgc cagcatatag ccaatcatcc cctggctttc cgccaccagt acatccagcg
     7981 gatacggttc tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc
     8041 cgttgccgtg aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag
     8101 cactggcgat attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg
     8161 cgttgccccc aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc
     8221 aagccagaac cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc
     8281 aatcgcaaca gcggtacgtt tcgggcaact cagagcatgg acaaagtgca ggagtggcga
     8341 ggcgaaatat ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag
     8401 ttgaagattg tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta aagcgccata
     8461 ataccaggcc gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg
     8521 gcctttaccc agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca
     8581 gtgacgaaac tggcgtaatt cagcgggcag cggatcgctt gccgccatcg ccagtggccc
     8641 gaaaagtccg gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcga
     8701 aggcggcgta atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag
     8761 gcgaatccct tgagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc
     8821 atcgaattgc atgcggcgaa attacacatt cccgtcaagc tatagaagaa acgctgccct
     8881 ggctttcctc ttttggcgct gacccaacgg gtgggatgac ccgtttactt tattcgccgg
     8941 aatggctgga aacccagcag caatttaaaa aaagaatggc agcaagtggg ctggaaacac
     9001 gtttcgatga agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag
     9061 tggttctgag cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat
     9121 tcggcgcgct ggcggcgtgg ctggcaattg actggttgaa aacgcaatac ggcgcaccgt
     9181 tacgtacggt cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct
     9241 tctggggcag taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg
     9301 atgccaaagg aaatagtttt gccgatgcga tgaaggcttg cggatttact ctgccgaacg
     9361 ccccactaac tccgcgtcag gctataaaag cctttgttga actgcatatt gaacagggct
     9421 gtgtgctgga aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc
     9481 gctatacggt gacgctgaac ggcgaatcaa accatgcagg caccacgccg atgggttatc
     9541 gtcgtgatac ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa aaagcgaaaa
     9601 agatgggcga tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa
     9661 atgtggtgcc gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc
     9721 tgcgcgattt cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca
     9781 ttggtattga tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg
     9841 tcgccaccct gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg
     9901 gtgccgggca cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg atttttatcc
     9961 ccagcatcaa cgggatcagc cataacccgg cggaacgcac caatattact gaccttgccg
    10021 aaggggtcaa aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca
    10081 taaatgggat atttaaataa cgtcaccggt taccgcgaag atttactggc taaccgtgcg
    10141 attgttaaac acggtaattt cgcactgtta accccggatg gtctgataaa aaatattatt
    10201 ccggactttg aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt
    10261 gttgattatc tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa
    10321 ggcattgaaa cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa
    10381 acatttgcct taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt
    10441 gttaacgccc aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta
    10501 gaaggccatg caccgtggct gttttctggc aatgccagcg aactggaacg cattcactat
    10561 gaaggtatgg atgatgtgat cctgctggat tttctgccca aagagttagg ttttgatatg
    10621 aacatgcata tcctctcttt tgcaccaggt gccagccacg gttatatcga aacacacgtt
    10681 caggaacatg gtgcctatat tctttccggt cagggggttt ataac
//
LOCUS       DAJTRB010000125        10564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTRB010000125 DAJTRB010000000
VERSION     DAJTRB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.125"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="OO330_003598"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="OO330_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5884326.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="OO330_003599"
     CDS             complement(2005..2928)
                     /locus_tag="OO330_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5884327.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="OO330_003600"
     CDS             3145..4488
                     /locus_tag="OO330_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5884328.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="OO330_003601"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="OO330_003601"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5884329.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="OO330_003602"
     CDS             6508..6732
                     /locus_tag="OO330_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5884330.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="OO330_003603"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="OO330_003603"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5884331.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8293)
                     /locus_tag="OO330_003604"
                     /pseudo
     CDS             complement(7326..8293)
                     /locus_tag="OO330_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8417..9409
                     /gene="tehA"
                     /locus_tag="OO330_003605"
     CDS             8417..9409
                     /gene="tehA"
                     /locus_tag="OO330_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5884332.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMSIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9406..9999
                     /gene="tehB"
                     /locus_tag="OO330_003606"
     CDS             9406..9999
                     /gene="tehB"
                     /locus_tag="OO330_003606"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5884333.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10302..>10564
                     /locus_tag="OO330_003607"
     CDS             10302..>10564
                     /locus_tag="OO330_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HCR5884334.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQ"
BASE COUNT         2717 a         2470 c         2655 g         2722 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaata attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 taatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgctatc acatcgttaa aatcgctaat ggcgataatc tgccgtaata
     8221 acacactttt tttagtgcca tcttcctgat aactaaaggc tcgctgttct tcacttaggc
     8281 gatatttgcg catagcgttt tcccacaggt gacttactat aaccgtagca aattctgcgg
     8341 ctctggctat gctcgagaaa attccataaa atgcatttca aatatacttt ataaattaaa
     8401 caaaatgagt aagaagatgc agagcgataa agtgctcaat ttgccggcag gctactttgg
     8461 tattgtgttg gggacgatag ggatgggatt tgcctggcgc tatgccagcc aggtttggca
     8521 ggtcagccac tggttagggg atgggctggt gattctggcg atgagcatct ggggattatt
     8581 gactagcgca tttattaccc gactcatacg ctttccgcat agcgtgctgg cggaagttcg
     8641 ccatccagtg ctgagcagtt ttgttagttt gtttccggca acgacgatgc tggtggcgat
     8701 tggttttgtt ccgtggtttc gcccactggc ggtgtgcatg ttcagttttg gtgtcgtggt
     8761 tcagttggct tatgccgcct ggcaaactgc gggattatgg cgcggatctc accctgaaga
     8821 agctaccacg cctggactgt atctgccgac agttgccaac aactttatca gcgcaatggc
     8881 ctgtggtgcg ttgggctaca ccgacgccgg tctggtgttt ttaggcgcag gcgttttctc
     8941 atggctaagc cttgaaccgg tgatcttgca gcgtctgcgc agttcgggag aattacccac
     9001 ggcactgcgg acatcactcg gcattcagct cgctcctgcg ctggtggcct gtagtgcctg
     9061 gctgagcgtc aacggcggcg agggtgacac gctggcgaaa atgcttttcg gttatggact
     9121 gctgcaactg ctgtttatgc tacgtctgat gccatggtat ctctcccagc catttaatgc
     9181 ttcattctgg agtttctcgt tcggcgtatc tgcactggca accaccggtt tgcatctggg
     9241 gagtggcagc gataatggat ttttccatac gctggcggtg ccgctgttta tctttaccaa
     9301 ttttattatt gcaatactgc tcatccgtac ttttgcgctt ctgatgcagg gaaaattgtt
     9361 agtcagaacc gagcgcgccg ttttaatgaa agcagaggac aaagaatgat cattcgtgac
     9421 gaaaactatt ttactgataa atatgaatta acccgcacac actctgaagt actggaagcg
     9481 gtgaaagtgg ttaaaccggg taaaacgctg gatctgggct gtggcaatgg tcgtaacagt
     9541 ctttacctgg cagccaatgg ttatgatgtt gacgcatggg ataaaaatgc catgagtatc
     9601 gccaacgtcg agcgcattaa atccattgaa aatctggata atttacacac ccgggtcgtt
     9661 gatctgaata acctcacatt tgatggacag tacgatttta ttctttcgac tgtggtgctg
     9721 atgttccttg aggctaaaac catcccaggg ttgattgcca atatgcaacg ttgcactaaa
     9781 cctggtggtt acaacctgat tgtggcggcg atggataccg ctgattatcc atgtaccgtc
     9841 ggcttcacgt ttgctttcaa agagggagaa ttacgtcgat attacgaagg ctgggagagg
     9901 gtgaaataca atgaagacgt cggcgagctg caccgcaccg acgccaacgg taatcgtatt
     9961 aaactgcgtt tcgccacgat gctggcacgt aaaaaatgac ccggtaagca caaaacgcgt
    10021 gaaaattccc cacgctgaga tgatttactg ttcttctttt cggtaagcat attttttatc
    10081 gaagggatgt gaaattaatc acagtagtcg aagtttttta gcagcttaac ttactgaaat
    10141 ttaactactg atgattgact tagccccttt ttcggcattg actatgtcgt ctgaaaaggg
    10201 gctgaaaaat ttattttcac caacactttt tttgccacaa cacgaagcgg cgctttttgc
    10261 tataacttag aaagtaatat aatcatctca ggaaactatt catgcgtacc acatcatttg
    10321 cgaaagttgc agctttatgc ggcttgttgg ctctgtctgg ttgtgcctct aaaatcaccc
    10381 agccagataa atattctggt tttttaaaca attactctga tttaaaagaa acaacctcag
    10441 caacaggtaa acctgtttta cgttgggtag acccgagttt tgatcaaagc aaatatgaca
    10501 gcatcgtctg gaacccaatc acttattatc cggtaccaaa accttctacc caggtagggc
    10561 agaa
//
LOCUS       DAJTRB010000126        10294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTRB010000126 DAJTRB010000000
VERSION     DAJTRB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.126"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..401
                     /locus_tag="OO330_003608"
                     /pseudo
     CDS             1..401
                     /locus_tag="OO330_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="OO330_003609"
     CDS             484..741
                     /locus_tag="OO330_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884335.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO330_003610"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO330_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5884336.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO330_003611"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO330_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5884337.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO330_003612"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO330_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5884338.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO330_003613"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO330_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5884339.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO330_003614"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO330_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5884340.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO330_003615"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO330_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5884341.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO330_003616"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO330_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5884342.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9142..9324
                     /locus_tag="OO330_003617"
                     /pseudo
     CDS             9142..9324
                     /locus_tag="OO330_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9318..9422
                     /locus_tag="OO330_003618"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="OO330_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="OO330_003619"
     CDS             9762..10019
                     /locus_tag="OO330_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5884343.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2401 a         2735 c         2822 g         2336 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaatgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggc tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agca
//
LOCUS       DAJTRB010000127        10075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTRB010000127 DAJTRB010000000
VERSION     DAJTRB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.127"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            39..170
                     /locus_tag="OO330_003620"
     CDS             39..170
                     /locus_tag="OO330_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5884344.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            430..1425
                     /gene="pgl"
                     /locus_tag="OO330_003621"
     CDS             430..1425
                     /gene="pgl"
                     /locus_tag="OO330_003621"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5884345.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1466..2419)
                     /locus_tag="OO330_003622"
                     /pseudo
     CDS             complement(1466..2419)
                     /locus_tag="OO330_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2603..3655
                     /locus_tag="OO330_003623"
     CDS             2603..3655
                     /locus_tag="OO330_003623"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5884346.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLFGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3731..5164
                     /locus_tag="OO330_003624"
     CDS             3731..5164
                     /locus_tag="OO330_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5884347.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5347..7527
                     /locus_tag="OO330_003625"
     CDS             5347..7527
                     /locus_tag="OO330_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5884348.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGLQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7668..8951)
                     /locus_tag="OO330_003626"
     CDS             complement(7668..8951)
                     /locus_tag="OO330_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5884349.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9086..9967)
                     /locus_tag="OO330_003627"
     CDS             complement(9086..9967)
                     /locus_tag="OO330_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5884350.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2440 a         2348 c         2557 g         2730 t
ORIGIN      
        1 acttttgacg cagtgtcttg ttaacagggg gattgaccat gcttattggc tatgtacgcg
       61 tatcaacaaa tgaccagaat accgatctac aacgtaatgc gtttatttgt gccggaagtg
      121 agttgattgt tgcgcgaatg ggaagttggc cagaagttgt tatggcatag tgtttacggt
      181 cgttgataca ggtaaacaaa agcggggaga gtcatactcc ctgaatagtc gcgaaaatat
      241 ctgtggcatt gtcaaattcg tgtcggtgac taatatttta gtaatatgcc attcgaaatg
      301 gagtgctaac ttattgattt aattgatgtg cgatattgta ttttatcgtg ttttgttgtc
      361 tgttttttat ccagtaagtg ttgttatatg tatgaaagat aagggattta tcatttttga
      421 ggtgtgttca tgaagcaaac agtttatatc gccagccctg agagccagca aattcacgtc
      481 tggaacctga atcatgaagg cgcactgacg ctgacacagg ttgtcgatgt gccggggcag
      541 gtgcagccga tggtggtcag cccggacaaa cgttatctct atgttggtgt tcgccctgag
      601 tttcgcgtcc tggcgtatcg tattgccccg gacgatggcg cactgacctt tgccgcagag
      661 tctgtgctgc cgggtagtcc gacgcatatt tccaccgatc accaggggca gtttgtcttt
      721 gtaggttctt acaatgcggg taacgtgagc gtaacgcgtc tggaagatgg cctgccagtg
      781 ggcgtcgtcg atgtagtcga ggggctggac ggttgccatt ccgccaatat ctcaccggac
      841 aaccgtacgc tgtgggttcc ggcattaaag caggatcgca tttgcctgtt tacggtcagc
      901 gatgatggtc atctggtggc gcaggaccct gcggaagtga ctaccgttga aggggccggc
      961 ccgcgtcata tggtattcca tccaaacgaa caatatgcgt attgcgtcaa tgagttaaac
     1021 agctcagtgg atgtctggga actgaaagat ccgcacggca atatcgaatg tgtccagacg
     1081 ctggatatga tgccggaaaa cttctccgac acccgttggg cggctgatat tcatatcacc
     1141 ccggatggtc gccatttata cgcctgcgac cgtaccgcca gtctgattac cgttttcagc
     1201 gtttcggaag atggcagcgt gttgagtaaa gaaggcttcc agccaacgga aacccagccg
     1261 cgcggcttca atgttgatta cagcggcaag tatctgattg ccgctgggca aaaatctcac
     1321 cacatctcgg tatacgaaat tgttggcgag caggggctac tgcatgaaaa aggccgctat
     1381 gcggtcgggc agggaccaat gtgggtggtg gttaacgcac actaaccgct gatttaaccg
     1441 gcgcagtctc tcctgcgccg gtgtattaac ttatctcctg taacgcatgt ctctggcgtt
     1501 cgacgatatt ggtccacaaa ttgtctttat cgtcagtcca caaattaatc agcaaggaaa
     1561 aaaagcgttc agctgccgga gaaagcacgg catctttgcg gcgaataatc cccaatgtcc
     1621 gacgtataac gggttccacc agtgggatac caataatcga agaatagggc gcatggggca
     1681 ttgccagacc aggaagcgcc gaaatcccca gtcctgcctc cactagtcct aatgacgtcg
     1741 aaagatgacg cacttcgtaa aaccagtcca gcttccaggg cttgtcggct agctgttgtt
     1801 ctatcaacaa tcggttgccg ctggaggaac gtacgccaat cattttgtaa cccactaact
     1861 cctgccattc tacaagctac tttttggcca atggatggtc acgccgacag gccagcacga
     1921 atggctcgtt gaccagtggg gtaaaatcaa tggatgaatt tgtgacgttg ttcatgttta
     1981 tgccaaagtc tgattcgtta cacagcaccg attccatgca attattcgtg ccttgttcca
     2041 gaatccgcac tttaatattg ggatacagct cattaaattt accgattgcc agcggtaaaa
     2101 aataaaatac tgcggtcgga atacacgcta atgtcaccat accacgatga taagcattca
     2161 tatcacgaat attaaaaagc gtttcatcga atttctttat taattcccgc gcctccggga
     2221 gcaacctttt tcctgctttt gttaacgtaa ccttgcgagt tgtacgctca aatagttgaa
     2281 tatgtaaatc ttcctccatc ttttttattc ttcgcgttaa tgcaggttgc gtaatattga
     2341 gtaatttagc ggcattgtta aatgaactgg actctgccag tatgacaaat gccttcatac
     2401 ttgataattc atgcttcatt attactccgg aaaatggaag cgacgatttt ggggggctgg
     2461 tcgttaaaaa ttttaactgc atttagccaa cttaaattaa tgaaaaaatg ttattaatcg
     2521 ttgagctaaa gtcattagag atgctttgtc cttaatgtaa ccatatcgca ataagttatg
     2581 tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc gaggtggaac
     2641 ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc agcgcgataa
     2701 aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta ttggcggcgg
     2761 taatccactg accagtaaag tcgccattat tagccgttcc agcgatccgc gtgctgatgt
     2821 cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca cgcctaactg
     2881 cggcaatatg ctgtttggcg taggggcatt cgccattgaa aatggtttga ttgcagcgac
     2941 ttcgccagtt acccgtgtac gtatccgcaa cgtcaatacg ggtacgttca tcgaagctga
     3001 tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg acggcgtacc
     3061 gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gctggaacga aaaccggaaa
     3121 agttttcccg actgataatc agattgatta ttttgacgat gtcccggtga cctgtatcga
     3181 tatggcgatg ccagtcgtca ttattccggc tgaatatctg ggaaaaacag gttatgaatt
     3241 accggcggaa ctggatgccg acaaagcatt attagcccgc attgaatcta tccggctaca
     3301 agcgggtaaa gcaatgggct taggtgatgt cagtaatatg gttatcccta aacctgtgct
     3361 tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc cgcattcttg
     3421 ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc agtagttgtg cattggaagg
     3481 caccgtcacc cgacaaatcg tcccttctgt aggatatggc aatatcaata ttgaacaccc
     3541 cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga cgttacgcgc
     3601 atctgttatt cggacaacca gaaaaatatt ttccggtgaa gtttatcttc cctgaaaaaa
     3661 ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata cagaaagaac
     3721 aggattttaa atgaataaga aatcgttatg gaagctaatt ctgatattag cgatcccatg
     3781 tattattggt tttatgccag ctccggcagg attaagcgaa ctggcgtggg tgctttttgg
     3841 tatttacctg gcggccattg tgggtctggt tatcaagcct ttcccggaac ctgtcgtact
     3901 gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg gtgcgtttaa
     3961 gaccaccgcc gtattaagcg gttactcttc aggtactacc tggctggtgt tctcggcgtt
     4021 taccttaagc gccgcatttg tgaccaccgg tttaggtaaa cttattgcct atctgctgat
     4081 tggtaaaatc ggtaacacca tgctgggtct gggttacgtt acggtattcc tcgatctggt
     4141 actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt taccgatcat
     4201 caacagcgtg gcggtggctt tggggtccga accggaaaaa agtccgcgtc gtgtcggaca
     4261 ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt tctttaccgc
     4321 aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc tgcaaattag
     4381 ctggggtgga tgggcgctgg ccgccggatt gccgggcatc attatgctgc tggtcacccc
     4441 gctggtaatt tacaccatgt atccaccaga aattaaaaag gtggataaca aaaccatcgc
     4501 taaagcgggc cttgccgaac tgggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt
     4561 ctttgtgctg gcgctactgg gctggatttt cagtaagtct ctgggggttg atgaatccac
     4621 tgtggcaatc gttgttatgg cgaccatgct gctgctgggt atcgttacct gggaagacgt
     4681 ggttaaaaat aaaggcggct ggaatacctt aatctggtac ggcggtatta tcggcttaag
     4741 ctccttatta tcgaaagtta aatttttcga atggttagct gaagtcttta aaaataacct
     4801 ggcatttgat ggtcacggta acgttgcttt cttcgttatt attttcctca gcatcatcgt
     4861 gcgttatttc ttcgcttccg gtagtgccta tatcgttgct atgttacctg tatttgccat
     4921 gctggcgaac gtctccggcg caccgttaat gttaaccgcg ctggcactgt tgttctccaa
     4981 ctcctatggc ggcatggtta ctcactatgg cggcgcggca ggtccggtca tctttggcgt
     5041 gggttacaac gatattaaat cctggtggtt ggtcggtgcg gtactgacga tattaacctt
     5101 cttggtgcat atcaccctcg gcgtgtggtg gtggaatatg ctgatcggct ggaacatgct
     5161 gtaaatatac tcgtcatact tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt
     5221 cacttactta tgtaagctcc tggggattca ctcacttgtc gccttcctgc aactcgaatt
     5281 atttagagta tatccattta ttatctttct gcgcacttca cggtgcgcag atatctggag
     5341 catttgatga tcaagttatc tgaaaaaggc gtgtttctcg ccagtaataa cgaaataatt
     5401 gccgaagaac atttcaccgg cgaaattaaa aaagaagaag caaaaaaagg cactattgcc
     5461 tggtctattc tctcttctca taatacgtcc ggaaatatgg ataaacttaa aattaagttt
     5521 gattcattag cctctcacga tattaccttt gttggtattg tacagaccgc taaagcgtcc
     5581 ggaatggaac gtttcccgct gccgtatgtg ctgaccaact gccataactc actctgcgcc
     5641 gtcggcggca ccattaacgg tgatgaccat gtttttggtt tatcggcagc tcagcgttat
     5701 ggcggtattt ttgtgcctcc gcatattgcg gtcatccatc aatatatgcg tgagatgatg
     5761 gctggcgggg gcaaaatgat cctcgggtca gacagccaca cccgttacgg tgcattaggg
     5821 acaatggcag tcggtgaggg tggcggtgag ttggtaaaac agctgcttaa tgacacctgg
     5881 gatatcgact atccgggcgt ggttgcggtg catctgaccg gaaaacctgc gccgtatgtg
     5941 gggctacagg atgtggcgct ggctatcatt ggcgcggtgt tcaaaaacgg ttacgtcaaa
     6001 aacaaagtca tggagttcgt tggaccgggc gttagcgcgc tctctaccga tttccgtaac
     6061 agcgttgacg tgatgaccac ggaaaccacc tgtttaagct ccgtctggca aactgatgaa
     6121 gaagtgcata actggctggc gctgcacggt cgcggccagg attactgcca gcttaaccct
     6181 caaccgatgg cgtactacga tggctgcatc agcgttgatt taagcgccat caaaccaatg
     6241 attgcgctgc cgttccaccc gagcaacgtg tatgaaatcg acacactgaa ccagaacctg
     6301 accgacattc tgcgtgagat tgaaattgag tccgaacgcg tggcgcacgg taaagccaaa
     6361 ctctcgctgc tggataaagt ggaaaatggt cgcctgaaag tgcagcaggg gattatcgcg
     6421 ggctgttctg gcggtaacta cgaaaacgtc atcgcggcgg cgaatgcact gcgcggtcaa
     6481 tcctgtggca atgacacctt ctcgctggcg gtttacccgt catcacagcc ggtgtttatg
     6541 gatctcgcca aaaaaggtgt ggtagcagat ttgattggcg caggcgcaat catcagaacc
     6601 gcattctgtg gcccatgctt tggcgcgggc gatacgccaa tcaacaacgg tttgagtatt
     6661 cgccacacca cgcgtaactt cccgaaccgc gaaggctcta agccagccaa tgggcagatg
     6721 tcagcggtgg cgttgatgga cgctcgttct atcgctgcga ctgcggcaaa cggtggctat
     6781 ttaacctctg ccagcgaact tgattgctgg gacaacgtgc cggagtacgc cttcgatgta
     6841 acgccgtata aaaaccgtgt ttatcagggc tttgtgaaag gggcaactca gcaaccgctg
     6901 atttacggac cgaacattaa agactggccg gaattgggtg cgctgactga caatatcgtc
     6961 ctgaaagtgt gctcgaagat cctcgacgaa gtcaccacca ccgacgaact gattccttcc
     7021 ggtgaaacct cttcttatcg ttcaaatccg attggtctgg cggggaatgt cagcgagctg
     7081 acagaggtgt ttgcgcgcat taagcagatt gctggtcagg agcatattga tccgctgcaa
     7141 actgaaattg gcagcatggt ctatgcggtg aaaccaggcg atggttctgc gcgtgaacag
     7201 gcggcgagct gccagcgtgt gattggcggt ctggcgaata ttgccgagga gtacgcgact
     7261 aaacgctacc gttctaacgt catcaactgg gggatgttac cgctgcagat ggcggaagtg
     7321 ccaacctttg aagtggggga ttacatttac atccctggca ttaaagcggc gctggataat
     7381 ccgggtacga cgtttaaagg ttatgtgatc catgaagatg cgccggtaac ggaaattacg
     7441 ctttacatgg aaagcctgac ggctgaagag cgcgagatta tcaaggcggg tagtttgatt
     7501 aacttcaata aaaaccgtca gatgtaaaaa gcgccatgtg aatgtaggtc gcattcggca
     7561 cttattgtcg gatgcgatgc tggcgcatct tatccgacct acaaatcgca tcgaaccgta
     7621 ggccggataa ggcgtttacg ccgcatccgg caaatagtta atagctctta cttcttcgcc
     7681 tctgcaacca ctttgctgcc cacgccgcgg ttattgtatt cccacatgcg gttgtagtta
     7741 gtgtcattca gattgcgctg tatttcgtcg ttatcatcta cgctgccggt attacccgca
     7801 aacggacgat tagagatcac cgcatcggcc cacggtttag ccgtgttaaa accttcgttg
     7861 atggcgctat cacggatcac cacctgaccg ttggtattag catcaacatc cagcgagcgg
     7921 cccagttgcg ccacgccatc accggaagca ttgaaacggc tgtttacggc gaggaaaccg
     7981 taatagatgt tagacagcgt agccggtgca aacacatatg cttcttgctg ggtacgtgag
     8041 ttcaccacgc ggaattcggt gttatcgaac accactgcgc cgcgaccaga aacgatatcc
     8101 acatcccctt caatatagct gttggtcacc agcgtacgcg gctgacggtt ggtttccaga
     8161 cggttctgca caccgctgtt ggtgacaaag aaggtgttct gacgaccgag aatgttaacg
     8221 ttgttaatct gtacttggtc accgtcagta cgcagtgcta ccgccggatg gttacctgca
     8281 tctacgctat cgcccagcgt gttttcgatg gtcagatttt gcagttgcag tccattgttt
     8341 tgtgaccaga agaccgcaga acacagaaca ccgatactgt cgctgcgttt gctctggcag
     8401 ctatcgtaca tataccacgc tggtttacct ggcatatatt tgccgcgcgg gttgacgtcg
     8461 tgacgccagt cggcagggct catgccccca tcaagggaaa gcccaatctt cacatcaatc
     8521 ggtttttcac ctgtaccgta cagagtgatt ccacctggag cagcagggac atacaccgtt
     8581 ccctgatact caccaggcat cacggcaata tactggcgct tgttggtgcg cttgataatt
     8641 gccgcatcta ccgccgcctg aatcgtggta tgcgttacac cttgagtacc cgccgggccg
     8701 acaacaaagt caggttgcgc aggcagggtt atcggggacg gattccacgc tgcagcacct
     8761 ggtgtcaggg atgcaaaata gtgttgcgca tcgaaattca gcgcttcttt tgccgacaga
     8821 atcgggcgag aagaggtacc aggcgcagtt tgatcagaag gacgttgatc gggtggtgtt
     8881 gagctacagg cggtcagcgt cacgccaaaa gccaatgcca gcgccagacg ggaaactgaa
     8941 aatgtgttca caggttgctc cgggctatga aatagaaaaa tgaatccgtt gaagcctgct
     9001 tttttatact aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag
     9061 gtcattatca gtcaaaataa aatcattatt tgatttcaat tttgtcccac tccctgcctc
     9121 tgtcatcacg atactgtgat gccatggtgt ccgacttgtg cccgagaaga tgttgagcaa
     9181 acttatcgct tatctgcttc tcatagagtc ttgcagacaa actgcgcaac tcgtgaaagg
     9241 taggtggatc cccttcgaag gaaagacctg atgcttttcg tgcgcgcata aaataccttg
     9301 atactgtgcc ggatgaaagc ggttcacgac gagtagatgc aattatggtt tctccgccaa
     9361 gaatctcttt gcatttatca agtgtttcct tcattgatat cccgagagca tcaacatgca
     9421 atgttgtagg gatggcaatt tttacgcctg ttttgctttg ctcgacataa agatatccat
     9481 ctacgatatc agaccacttc atttcgcata aatcaccaac tcgttgcccg gtaacaacag
     9541 ccagttccat tgcaagtctg agccaacatg gtgatgattc tgctgcttga taaattttca
     9601 ggtattcgtc agccgtaagt cttgatctcc ttacctctga ttttgctgcg cgagtggcag
     9661 cgaccgggtt tgttgttata tggccttcag ctattgcctc tcggaatgca tcgctcagtg
     9721 ttgatctgat taacttggct gatgccgcct tgccctcgtc tatgtatcca ttgagcattg
     9781 ccgcaatttc ttttgtggtg atgtcttcaa gtggagcatc aggcagcccc ctccttattg
     9841 ctttaatttt gctcatgtaa tttatgagtg tcttctgctt gattcctctg ctggcgagga
     9901 ttttttcgta gcgatcaagc catgaatgta acgtaacaga attatcactg ttgattctcg
     9961 ctgtcagagg cttgtgtttg tgtcctgaaa ataactcaat gttggcctgt atagcttcag
    10021 tgattgaggt gtactggcaa tagcggacac taccgtttgt tcttttttta agcag
//
LOCUS       DAJTRB010000128        10045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTRB010000128 DAJTRB010000000
VERSION     DAJTRB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.128"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..807
                     /locus_tag="OO330_003628"
     CDS             <1..807
                     /locus_tag="OO330_003628"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5884351.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="OO330_003629"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="OO330_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5884352.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="OO330_003630"
     CDS             2629..3948
                     /locus_tag="OO330_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5884353.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4246..4287
                     /locus_tag="OO330_003631"
     CDS             4246..4287
                     /locus_tag="OO330_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884354.1"
                     /translation="MVQLVDLARCVSF"
     gene            4325..5460
                     /gene="dosC"
                     /locus_tag="OO330_003632"
                     /pseudo
     CDS             4325..5460
                     /gene="dosC"
                     /locus_tag="OO330_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            5385..6002
                     /locus_tag="OO330_003633"
                     /pseudo
     CDS             5385..6002
                     /locus_tag="OO330_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            6260..6592
                     /gene="ddpX"
                     /locus_tag="OO330_003634"
                     /pseudo
     CDS             6260..6592
                     /gene="ddpX"
                     /locus_tag="OO330_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6606..7502
                     /locus_tag="OO330_003635"
     CDS             6606..7502
                     /locus_tag="OO330_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5884355.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            7499..8485
                     /locus_tag="OO330_003636"
     CDS             7499..8485
                     /locus_tag="OO330_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5884356.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            8478..9404
                     /locus_tag="OO330_003637"
                     /pseudo
     CDS             8478..9404
                     /locus_tag="OO330_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(9460..9891)
                     /gene="osmC"
                     /locus_tag="OO330_003638"
     CDS             complement(9460..9891)
                     /gene="osmC"
                     /locus_tag="OO330_003638"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5884357.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2424 a         2485 c         2542 g         2594 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc tttctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtacc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggattctttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct gggtccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc gcgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttacctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcgaagac cttcttccct gacttctcta
     1561 aagtgggcac actggttgta tttgttgcct tcattttgag ttatatgggc gtagaagctt
     1621 ccgcaactca cgtcaatgaa atgagcaacc ccgggcgcga ctatccactg gctatgttac
     1681 tgctgatggt ggcggcaatc tgcttaagct ctgttggcgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcgcagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggca attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 attctaccga tatgtatgtt gaattactgg ttgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgcttgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcacatat ggatatctgc
     2641 tcccgaaaca agaaattaac gattagaaga ccagcgatac tggttgcatt ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtt
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaatcccac cagccgagcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tccaacgtct cggcataaac
     2941 acggtctttt tccaggtcaa gccggacggt accgccctgt ggccatcgaa aattttgccg
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattc
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc tgaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga atcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgt aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaagg tatcacacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgcagacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaacca
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac taccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
     4081 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
     4141 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtctt
     4201 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
     4261 gatctcgcac gttgcgtttc gttttgattc cgtctgattt cccagcctta taagggtgtg
     4321 aattatggag atgtatttta aaagaatgaa agatgagtgg accggacttg tcgaacaggc
     4381 agatcctctc attcgtgcta aagccgcaga aattgccctt gcgcatgctc attatctgag
     4441 tatcgagttt tatcgaattg tccgcatcga cccgcatgcc gaagaattct tgagtaatga
     4501 acaagttgag cggcagttga aaagtgcgat ggaacgctgg attattaacg tgctttctgc
     4561 ccaggttgac gatgtcgaaa ggctaataca aatccagcat accgtcgcgg aagtgcatgc
     4621 ccgcatagga attccggtag aaattgtcga gatggggttt cgggtgctga aaaaaatcct
     4681 ctatccggta atcttctctt cggattattc cgccgcagaa aaacttcagg tctaccattt
     4741 ctcgattaac agtattgata tcgcgatgga agtgatgacc cgcgcgttta cctttagtga
     4801 cagtagtgcc tcaaaggaag atgaaaacta tcgtatcttc tcgttactgg aaaacgccga
     4861 agaagaaaaa gaacggcaaa tagcctcaat actttcatgg gaaatagata ttatctataa
     4921 agtcctgctg gattctgatt taggcagcag tttgccttta agccaggctg attttggcct
     4981 gtggtttaac gacattattt tagcggcatt gccgaagtgg gccatatctc ccgtctgatt
     5041 caggatttcg acggtatttt caatcaaacc atgcgtaaca ccaggatttt gaataacaga
     5101 agtttgcggg tgaaatttct attacagata agaaataccg tatcgcagat tattaccttg
     5161 ctgcgtgaat tgtttgaaga agtatcgcgc cacgaagtcg gtatggatgt actgacgaaa
     5221 ttacttaacc gccgtttcct gccgactatc ttcaaacgcg aaattgccca tgccaaccgg
     5281 accggtacac cgctgtcagt gctgattatt gacgttgata aattcaaaga gatcaacgat
     5341 acgtggggcc ataacactgg cgatgaaatt ctgcgtaaag tctcattcct ctcgcagaag
     5401 agattggtga aatcgaaaat attgggcgct gggtcatcgc ggaagcttgc cgtcagttag
     5461 cagaatggcg tagccagaat attcatatcc cggcgttatc cgtgaacttg tcggcgctgc
     5521 actttcgcag taatcagctg cctaatcagg tgtctgatgc aatgcaagcc tggggtattg
     5581 acggccacca gctgacggtg gaaatcacgg aaagcatgat gatggaacac gataccgaaa
     5641 tctttaagcg cattcagatc ctgcgtgata tgggcgtggg tttatcggta gatgattttg
     5701 gcacgggctt ttccggatta tcccgcttag tcagtcttcc ggtaacggaa atcaaaattg
     5761 acaaaagctt tgtcgatcgt tgtctgacgg aaaaacgcat cctggcctta cttgaagcca
     5821 ttaccagcat tgggcaaagc ctcaatttaa ccgtcgtggc ggaaggggtc gaaaccaaag
     5881 agcaatttga gatgctacgt aagatccact gtcgcgttat tcagggatat ttcttttccc
     5941 gcccccttcc cgccgaagaa attccaggct ggatgagcag cgtgttaccg ctgaaaatct
     6001 gacaaattcc tctcgcccgc actcgcgggt tttcctttaa ggtgacactg tcacttaaat
     6061 actgtgattt cagctacgat tccgggagat tccttcctta actttccctc ttgcaccaaa
     6121 actgcactac gctgcacaaa tgttgatcaa aagttaaaaa atataacttt aagccagcat
     6181 tctggcgctt atcccggcat ggcatgagat ctgcataagc ggaaagcgca gcaatttttg
     6241 tcttatacag gaaccgttta tgtcggatac caccgaactg gttgatttag ccgtgatctt
     6301 ccctgatctg gagatcgaat tgaaatatgc ctgcgttgat aacatcacag gtaaagctat
     6361 ttatcagcaa gcgcgttgtc tgctacataa agatgcgatt accgcgctgg cgaaaagtat
     6421 cagtatcgcc cagctgtcag gcttacaact ggtgatttac gatgcgtatc gcccacaaca
     6481 agcacaggcg atgttgtggc aagcctgccc agacccgcaa tatgttgttg atgtgacggt
     6541 cggttctaat cacagccgtg gcacggcgct cgacctgacg cttcgtgatg acgtggaggt
     6601 ggtgaatgat gctaagcgag gaaacgtccg ccgtacgccc acaaaaacaa acgcgattta
     6661 acggtgcaaa actggtatgg atgctgaaag gcagtccgct caccgtgacc ggcgcagtca
     6721 tcattgtatt aatgcgactg atgatgattt tttcaccgtg gctggcgacg catgatccca
     6781 acgccattga tttaaccgcc cgccttttgc cgccttctgc ggcgcactgg tttggcaccg
     6841 atgaagtggg acgcgatctg tttagccgcg tactggtcgg cagtcagcaa tcaattctcg
     6901 ccggattagt ggtggtcgcc attgcgggta tgattggttc gctactcgga tgtctatccg
     6961 gtgtgcttgg cggacgcgca gacgccatca tcatgcgcat catggacatt atgctgtcga
     7021 ttccttcgtt ggtactgaca atggcactgg ctgccgctct cgggccgagt ttatttaacg
     7081 ccatgctggc gattgctatt gtgcgaattc ccttttatgt gcgcttggcg cgggggcaag
     7141 cattagttgt acgccagtat acctatgttc aggcggcgaa aacctttggc gcgtctcgtt
     7201 ggcatctgat caactggcat attttacgta actccctacc gccgctgatc gtgcaggcat
     7261 cgctggatat cggtagcgcg attttaatgg ccgccacgtt gggatttatt ggcctaggtg
     7321 ctcaacaacc gagtgctgaa tggggcgcga tggtagcgaa tggtcgcaac tatgtactcg
     7381 atcagtggtg gtattgcgca tttccggggg cagcgatttt gcttacctcc gtcgggttta
     7441 atctctttgg tgatggtatt cgcgatctgc ttgacccgaa agcaggagga aagcagtcat
     7501 gacccaaccc gttctggaca ttcaacagct gcatttgagt ttccccggtt ttaacggtga
     7561 tgttcacgcg ctcaacaatg tgtccttgca gattaaccgc ggcgaaattg tcggtctggt
     7621 gggagaatcc ggctcaggta aatcagtcac cgcaatgctg attatgcgtc tgctaccgac
     7681 gggcagttat tgcgtacatc gggggcagat ttcactgctg ggagaagatg ttcttaacgc
     7741 tcgggaaaaa cagcttcgtc agtggcgcgg cgcacgagtg gcgatgatct ttcaggaacc
     7801 gatgaccgcc cttaatccga cacgtcgaat aggtcttcag atgatggacg taatccgcca
     7861 tcatcaacca ataagtcgtc gggaagccag agctaaagcg attgccctgc tggaagagat
     7921 gcaaatcccg gatgccgtgg aagttatgtc gcgctatccg tttgagcttt caggtggtat
     7981 gcgccagcgg gtaatgattg cgctggcatt ctcctgcgag ccgcaattga ttattgccgg
     8041 cgaaccgacg acggcgctgg acgtcacagt acagttgcag gtactgcgtt tgcttaaaca
     8101 taaagcacgc gccagtggaa ctgcggtact gttcatcagc catgatatgg ccgtggtgtc
     8161 gcaactgtgc gatagcgttt acgtgatgta tgccggaagc gtaattgaaa gcggcgggac
     8221 ggcagacgtt atccatcatc cccggcatcc gtataccatt ggtttgctgc aatgcgcacc
     8281 agaacatgga gtaccacgcc agccattacc cgccattcca gggacggtac caaacctcac
     8341 ccatttgcct gacggctgcg cttttcgcga tcgttgctat gcggcaggtg cacagtgtga
     8401 aaacgtcccg gcgctgacag cgtgtggtga caacaaccag cactgcgcct gttggtatcc
     8461 gcagcaggag gtcataagtg tctgacacgt tattaacgtt acgcgacgtc catatcaatt
     8521 ttccggcccg taaaaactgg cttggtaaat ctacggaaca tgttcatgcc attaatggta
     8581 ttgatttaca gatccgccgt ggtgaaacct tagggatcgt cggcgagtca ggctgcggca
     8641 aaagcaccct cgcacagctt ttaatgggca tgctgcaacc gagccacggg cagtacatcc
     8701 gttcaggctc acaacgcatt atgcagatgg tgtttcagga cccgctctct tcgcttaatc
     8761 cgcgcttacc ggtgtggcgc atcatcacag aatcgctctg gatagctaag cgtagtagtg
     8821 aacaacagcg gcgagcgttg gcagaggagt tggctgtgca ggtgggtatt cgtccggagt
     8881 atctcgaccg cctgcctcat gcgttctccg gcgggcagcg gcaacgcatc gccattgcca
     8941 gagcactctc ttcgcagcct gacgtgattg tgcttgatga gccaacctcg gcgctggata
     9001 tctccgtgca ggcgcagatc ctcaatttgc tggtaacgct acaggaaaat cacgggctga
     9061 cttatgtgct gatttcacac aatgtctcgg tgatacgtca tatgagcgat cgggtggcgg
     9121 tgatgtatct cgggcagatt gtagaactgg gcgacgcgca acaggtgttg actgcttcgg
     9181 cacatccata cacccgatta ttgctggatt ccctccccgc cattgataaa ccgctggagg
     9241 aagaatgagc attacgtaaa acggatctgc cgggaaaccg cacgttgccg caaggctgtt
     9301 ttttctacga acgttgccct ctggcaaccc acggatgtga agtccggcaa tcattagcaa
     9361 taagggagga cggacgtgag ctccggtgct ggcgggcgct gtagagatgc taacaacgca
     9421 tcaggcattc acgcgcctga cgcgtcatcc ggcaatgctt tacgatttca actggtaatc
     9481 cagcgtaatt tccgctttca gtacctgaga gaccgggcat cctgcttttg ctttctggat
     9541 gatgccgtca aaggtagagg catcaatacc cggcaccgca acttcactct tcagtgcgat
     9601 tttcgtaatc gcaaaaccgg catccacttt atccagcgac acatcggcgg tggtatcaat
     9661 tgatgttggc gtgaatcccg cttcccccag cattaatgaa agcgccattg agaaacatgc
     9721 ggcatgcgct gcgccaatca gttcttcagg gttggttcct ttttcgcctt caaaacgcgt
     9781 gttaaatcca tacggctgtt ggttcagcac gccgctctcg gtggatactg ttcccttccc
     9841 gcgtttgata tcgccttccc agtgtgcctg acctttctta tggattgtca ttgttgctct
     9901 cctgtgggct taaaaaggaa attatagact acgtgagaaa cagcacgagg ataagcagga
     9961 tattccgaat aaaatacggc gaaaatatta cttctgcact taaattaaat tcccacaata
    10021 gataaccggg aatatatatc tcaag
//
LOCUS       DAJTRB010000129         9951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTRB010000129 DAJTRB010000000
VERSION     DAJTRB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.129"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..344)
                     /locus_tag="OO330_003639"
     CDS             complement(<1..344)
                     /locus_tag="OO330_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5884358.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGP"
     gene            complement(410..1039)
                     /locus_tag="OO330_003640"
     CDS             complement(410..1039)
                     /locus_tag="OO330_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884359.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1043..1258)
                     /locus_tag="OO330_003641"
     CDS             complement(1043..1258)
                     /locus_tag="OO330_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5884360.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGMLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(1551..1625)
                     /locus_tag="OO330_003642"
     tRNA            complement(1551..1625)
                     /locus_tag="OO330_003642"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1590..1592),aa:Gly,seq:tcc)
     gene            complement(1629..1704)
                     /locus_tag="OO330_003643"
     tRNA            complement(1629..1704)
                     /locus_tag="OO330_003643"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1669..1671),aa:Thr,seq:tgt)
     gene            complement(1707..1783)
                     /locus_tag="OO330_003644"
     tRNA            complement(1707..1783)
                     /locus_tag="OO330_003644"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1747..1749),aa:Arg,seq:tct)
     gene            complement(1785..1861)
                     /locus_tag="OO330_003645"
     tRNA            complement(1785..1861)
                     /locus_tag="OO330_003645"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1825..1827),aa:Ile,seq:cat)
     gene            complement(2061..2441)
                     /locus_tag="OO330_003646"
     CDS             complement(2061..2441)
                     /locus_tag="OO330_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5884361.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDNAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(2434..2634)
                     /locus_tag="OO330_003647"
     CDS             complement(2434..2634)
                     /locus_tag="OO330_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5884362.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(2729..2986)
                     /locus_tag="OO330_003648"
     CDS             complement(2729..2986)
                     /locus_tag="OO330_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884363.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(2983..4374)
                     /locus_tag="OO330_003649"
     CDS             complement(2983..4374)
                     /locus_tag="OO330_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5884364.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(4371..5249)
                     /locus_tag="OO330_003650"
     CDS             complement(4371..5249)
                     /locus_tag="OO330_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5884365.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(5260..6168)
                     /locus_tag="OO330_003651"
     CDS             complement(5260..6168)
                     /locus_tag="OO330_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5884366.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(6155..6388)
                     /locus_tag="OO330_003652"
     CDS             complement(6155..6388)
                     /locus_tag="OO330_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884367.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(6385..7401)
                     /locus_tag="OO330_003653"
                     /pseudo
     CDS             complement(6385..7401)
                     /locus_tag="OO330_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            7333..7596
                     /locus_tag="OO330_003654"
     CDS             7333..7596
                     /locus_tag="OO330_003654"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5884368.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            7586..7894
                     /locus_tag="OO330_003655"
     CDS             7586..7894
                     /locus_tag="OO330_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5884369.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(7936..8112)
                     /locus_tag="OO330_003656"
     CDS             complement(7936..8112)
                     /locus_tag="OO330_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR5884370.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(8109..8519)
                     /locus_tag="OO330_003657"
     CDS             complement(8109..8519)
                     /locus_tag="OO330_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5884371.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(8516..8794)
                     /locus_tag="OO330_003658"
     CDS             complement(8516..8794)
                     /locus_tag="OO330_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884372.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(8869..9693)
                     /locus_tag="OO330_003659"
     CDS             complement(8869..9693)
                     /locus_tag="OO330_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884373.1"
                     /translation="MVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLEL
                     KPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARR
                     RYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKP
                     TGKMDVKGSGAITGLTDKLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLML
                     EKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         2395 a         2682 c         2260 g         2614 t
ORIGIN      
        1 gggccaatgt tataggggca aaatgacgct atacccgctt tctggggttc ggtcagaggt
       61 actttaatat tgcgctccac ccatgccagc gccttatcac gttcaatagc gttaacctgg
      121 tcacattttt ccttcgacag cttcattccc ggtatgacag gcttaccatc caccattgtg
      181 gcaccacgac agatggtcca tatgccagaa ccatcacggt atgccgttgt gtgattaccc
      241 tctttttcgt tcagaaactg gtcaagtatc tggggagcag acgcgcctgc ggcaatcagc
      301 gccagaacag cagccgacag gccgtatttg atttttgcgc tcatggatat ttatcaggat
      361 ttatcggcaa caaataacga gccaacttat atacgccttt taagataagt cagccccggc
      421 tggatccagt catcaggctc tttcttaaag ggggagtatt gaaatcacga agaagagcct
      481 cccgcaccgc agcatccatg tctgcaccac tggcaagtgc ttcaatctct gccgccacct
      541 gcaaataccc catgcaacgg ccaatgcgtt tcataactct ggtgttttcg tctttcaggc
      601 atctgatagc gagattaata attttcaggc agtgatgatg cgatgcccgg acaggttttc
      661 cgttctcata aacgacgaac tcaccctctt cacaggaaac cctgaaaggc cgatggggtt
      721 cctggtcttt atccggtgca gacgcaataa aatcactttt atgctcttcc tctccggcac
      781 tgttaaccgt aatgacgtac tcaggccgca ggtaagccgc ctctttttct gacaatgcgg
      841 ccggtaaaaa cttttcctgc aaaaacttca taaaagtctc gtgagcgact ttgtggcagt
      901 aatcgtaaat cgccgcataa tgctcatcgc ggcgtttgtt ttcatcttca gaactcacca
      961 acgccgacaa ttttttatta agctcagtga tttcattttc caggtgattg aatcgctgat
     1021 tcatttcttc atgcttcatt atttactctc cccgtgcagc cttacgcttg tcttctctta
     1081 ttttgaaata cagattcgtc agataagtca gaagccccag aagcagactt cccagcatac
     1141 caatcgcagc ccactgtgat ggcctgacct gatccaacca ctgcaaaaac cagtagccgg
     1201 cactgccggc ggaggtgccg taggcaatgc ccgttgaaat tttgtccatg gatttcatag
     1261 cctcacctcc gcaaataacg gatggcgtag ttttacactg agaaatgaaa gggatttgaa
     1321 aagaaaaaaa cgcaaaagcg ggcgaaacga tatatacagt aaggaaagca ctctatccaa
     1381 caaaccaccc acagttaatc ggaataaaag cagagtgctt atgaatgatc gcacgcccga
     1441 aggttagtat ttctgcacag caattttgca aaaaaaggcg atcattcata acttaaacgt
     1501 ctttcagtca ctccgggatt tcccatcatc gcagactgaa agactctgac tggagcgggc
     1561 agcgggaatc gaacccgcat catcagcttg gaaggctgag gtaatagcca ttatacgatg
     1621 cccgcatatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
     1681 ctctgcctgc tgagctaagt cggcgctggc ccaccaccga ggactcgaac ctcgcaccgt
     1741 caacttagaa ggttgatgct ctatccggat gagctagtgg tggttggtgg cccttgctgg
     1801 acttgaacca gcgacctggc gattatgagt cgctcgctct gaccaactga gctaaagggc
     1861 cggaggcaga ataataacca tatgtcatca catctgcaaa ctcatctgac caccagcgtg
     1921 tttaacgtcc tgtaccgttt ttcaggcata aaaaacccgc ccggaggcgg gtttaagctg
     1981 tgtggcgaag caaccactct taacagaata tcccgttttt tacgtacgta aaatattttc
     2041 tagaaagtcg ccccttacca tcagggatgt tcaatatatt tgtccatttc taaccggaca
     2101 cccaacatca tcaacattcc ttccaccacc ccctcggctt tttgtagctt ctttcctata
     2161 taaccatctg agcatccatg cttccgcgcc agcgtcatga atgtcatccc aaacacgtag
     2221 tagtcaacca gcaagtcatg tagatcgttg ttgttccggt taaggcgggc catacaccca
     2281 catataacca tcgcgttatc gtcacagcat tgcgggcggg attttacttt tgaagggatt
     2341 aatcccttaa aaccggcagc aatggacgac caggtcacat cctcatgatt attagccacc
     2401 cacgcgcccc agcgctcaag aacctgctga atatcacgca tcactgtctt tacccctgtc
     2461 ccatccacga tgaacaatca gaacaccatc aacaatggcg tgccctttgc cctctttatc
     2521 ttcagcatat tttcttaccg tggcgcgatt acagttcagc attcgtgaaa cttcggtcat
     2581 attacctcgt gtctggataa gcagttccgg tatcgtttga attttgacgc tcatcaaata
     2641 ctctccggtt cggtgatttt tatcccgggc tttccaccag ggacataatg atcgcgcaca
     2701 agccagggca aaacaaactc atggcgcatc agcgcagcgc ctcctgcacc agtttttcaa
     2761 actttccgac tctggtttcc agctctgcca cacagtccac cagctcatct actgctttct
     2821 gtgcgcgatg cttcgcctgc atcagttccc gaagcgcggg taccatatct ttacggatag
     2881 catcttttgt tatgcccgtt ttttcgagtt gttcagcatg acgcagcatt tcctgcgcgt
     2941 gtttacgcaa ttgttcaggg gtaaaagtca ttgtctggtt gttcaaaaga aacgctccat
     3001 cttactgctg tcagttcgtt tattactgta tctgcgcgga ttgccgggct tcatgggagt
     3061 ggaaagcacc cgtgcacttt cctggtccac aggcagaaaa tgtccgttat aaaaacgccg
     3121 gtaaatcgtt cccagagaac cgttacgttg tttcgtgata ttgatttctg cgatgcccct
     3181 ggcctgcgta tccgggttgt acacttcatc cctgtaaagc atcagaatga tgtctgcatc
     3241 cgcctctatt tctccggaat ttttcaggtc tgagttcatg ggacgtttat tgggtctgga
     3301 ctccacaccg cgggagagct ggctcagcgc aatcaacgga aaaccaccgg attttgccag
     3361 gcctttaagc ccctttgaga tttcacccac ggcaaggtca tgacgccccg tggttcgggt
     3421 ttttatcagc ccgagataat caaccaccac cagcgccgtt tccggatatt taatcaggtg
     3481 gtgtttcgtt gttgcgcata tctcatcaat ggccaggttc gcctggtcca ccatccagat
     3541 attgcgcccg gtcatccgcc ccaccccttg tgagaaacgc gtccagtctt catcttcaaa
     3601 gtgagccaca gatttcaggc gtgatactgg catccctcca gccgcagaca ccatacgttc
     3661 accaatctgg atgttcgcca tctccatggt gaacagaagc acaccatgcc cctgctcagt
     3721 caccttgtcg atgatgtcca gcgcaagttc ggttttcccc atcgaaggac gggcggcaat
     3781 gaataccagg tctccgggct ccataccgcc tgtttttgcg tccagttcat caataccggt
     3841 catcaacgtc ctggatttct ccagcccctg attccggcat tcaacacgct caaccacttc
     3901 cggaagcaca tcatcaatat gtaccggctg aatgacgccc tttcctgtcg acagtgtgac
     3961 catcatgttc tgcgcatcct tcagggcatc ttcagctgct tcacaggtat gcgcatcacg
     4021 taatttctgc agcgcctcat tcagtgtttt ttctgcatcg cgcagtgcgg cattgcgccg
     4081 caacgctgca acatagtgct ccagtgaaga cttcacccag gttttacgcc cggtatcagt
     4141 aatcaccggg gcaagttccg gcatctcatt acacaacagc acggggtcaa tcactcctga
     4201 aacacgggcc tgtctgcaga tgcctgtata gatatcctga tacgctcgta cagaaaaaac
     4261 gtccgctggc agtgtggcca gaatatccat cacttcagga tctgctccgc gcagaaagaa
     4321 cgcgccgatg acagcgcctt caaggtcatc gttacgccat actggagttg tcatgcagcc
     4381 acacctctga tacgagaacg gtaactgggc cagttaaacg acaaccagtt gcgtcccccg
     4441 tctgtgatcc tgtcggcaat tcggggactg atgaacgccc acaactcttc cggtgagagg
     4501 ttactgatca gaatggtggg caagatactt tcgtacctgg cgttgataat ttcctgcaaa
     4561 atagccattt cagccgcgct gccaaactga acgccaactt cgtcgatgat cagcaaatcc
     4621 attgacgcat aacgctcaat aacttcatcc gctgtttttt cgctgtcatt ccgccagcaa
     4681 tttttcacag cacgggtaag gcgcatcacg tcggtgatct ccacactggc cagatagtta
     4741 cggatgatgt gttttgccat agccacagcc agatgatttt ttccggtacc acaactgccg
     4801 gtcataacaa gactggtacc gttctccagc atatctggcc agttctccgc atagcggcga
     4861 caggccgcaa gatttctggc tgcgccagga ttaacctcca gataattatc aaactcgcag
     4921 tcccgaaaac gcagggcaat tccggcgtta tcagtcagct cttccgcctt gatggacgac
     4981 agctccatgg tcaaatcgtt ggcctcagcg attaagcagt cagggcagca tgaaatttct
     5041 tctctgtcct cgccattacg atcgctccac accagtatat gcgtgtgata ttcgccatgt
     5101 ttttcgcaat atccgcgacc ttcacgcatc aggcaggaac gataaggcca tggcttttcg
     5161 cccttctgag caaatgcaat ctctgcccgt aactcatcca ttcgcgcctg tagtcttttt
     5221 tgttgttcac gcaggttaaa cgtcatcatc gctgtcacct cagaatgtca gtttgtcact
     5281 ggatttaccg aatttgtcag acatggctcc caggccagcc aggacatcga cctgtcgctg
     5341 tcgcccacct ctgggagcgg ctggctgttg ccagaagtct tcgaagtgac gatcgggtcc
     5401 aaagaacgtc gcagcctgct tcacgaactg tgtgccggta tttcctgtgg cacgtaccca
     5461 ggcagcatag cgtttcacgc catcaagcat ggcttcaggt tttattccct ccctgagccg
     5521 ggctttccag gctttgaagg ctgcggattt tgagttacca ccagcacgtt ttggatattc
     5581 ctgccaggcc tgttcaaatt ccggtgaata ttcctgtcgg gaagaacgcg ctggtgcaga
     5641 cgcgtcagcg gatgcgccaa tagtgttttt actctctgta gtattctctg aagtaatctc
     5701 tgttgtattc tctgtaagat cgaaattggt tttcccttct ccgcggcgag gggtttcccg
     5761 tgtccgcggt gaaggctttc cctcctccgc gaaattgggt tttacagttt cccgaaaacg
     5821 ggtttcccca tttcgggaaa actgattgtt ttcattgata atttcattaa ggcgctcaca
     5881 atctatacgg tagaacattt tgtgctcaag acgcttgttg gtttcaacca aaatgcctct
     5941 ggacacaaga tgcttacgcg ctacagcctg ttgttcaaat gtaagtccgg tttcgtgttg
     6001 tatctcttca cgcgttttat gtacgccttc cgctgcatgt gctttatcct gccagtaaaa
     6061 aatctgacca aagaaaataa cagcgtgcgg acttcccatg tatttaacga gcccagggta
     6121 ataagcaacc ggatgtccaa aatcgagcag aagatcagac ggacgcatag ccacctccca
     6181 ggcgtttaaa catttttccg ggctgaaacg ccaccagcgg ataactcagg gtatgagtac
     6241 gtccctgaac ctggcagaca accttctggc tttcggtact gaccaggcaa acccgcagaa
     6301 cgtagccgtt gctggtggtg aaccactgcc ccacacgggg gcaatggttg tatcggtgat
     6361 acaggaaatt aacgatgtgg cggatcatgg gcgcacctcc ttgtcagaac cattcagcct
     6421 ggaatcaaca agtgcagcac caaaaacagc atcacctaca cggtcgtaca gtttgccagc
     6481 cagcggagat tcaacggcct taagcattgg gtaaagctgg cttgtccaga tttgatggat
     6541 ttcacgcaaa tgcaggtata cgcctctggc gttttgtgcg acagatgaca tgtcagccgc
     6601 accagctcct gataagctcc tctccatctg gttaaaggca ttgatgtatg cctctttgaa
     6661 ccgggcggca cgtttaccag tgaagcccat agcaaggaag gcgaagccgc cgcgggtaat
     6721 ttggtagcag ggaagtttgc gagtaccgcc gttgggctgg cgtaccaaaa ttgatgtctc
     6781 cgcaaaattg cgggcacaaa actctggaga acaatccaaa atgcggatct ttttcagaac
     6841 atcgtcatga cgtttagaga agaagttggc aacagccagg gatgaagtaa cagcctaacc
     6901 atcaacgatg gcaatttcag gttgagagag ggttgggaga gtagtcatgg tgacagcccc
     6961 tatgttgaat tcaaagaact caccacatgg gacgccaatc acagaggtgg tgagacgtac
     7021 agggttggcg taaccggtca acatagaacc cggcgcatct tgcgatgtcc ctgcacgccc
     7081 caccataatt tgggcgtagc aatgctcatg acacgaaaaa accgcatgag cgcggttatg
     7141 ctctatattg aatttcagga cgccaatccc ggcccccgct ttataaggtg cagagacagt
     7201 gtaacgtccc gaaattgcag aatcaatata ttgtgcagat ataacgattt tgttatgttt
     7261 tgctgtatga actacactat cgaatactac agtgaagagg tccgactgga ggtcgatcag
     7321 cttccattga gtatgcgtgc cagataccag catcttgttg aacgcatgaa ggtatacggc
     7381 agcaatctcg gagaacctca caccagcgcc tttggtgacg ggcttttcga actcagaatt
     7441 aaaggcaacg atgggatcgc gcgtgttttt tactgcactc tgacagggaa acgcatcatc
     7501 atgctgcata gttttgtaaa gaaagcgcag aaaacaccgc cagccgaacg caagaaagct
     7561 gaaaccagaa tgaaggaggt taagcatgac tggtaaacgc actctcccca ccatgacaca
     7621 cgacgaaatg gcagccaaat ggatggaaga cccggctttt aaagcagaat acgacgctat
     7681 cgctgacgaa ttcgcactgc ttgatgaaat gctggcagca cgcaaagaag ctggcttaac
     7741 tcaggctgaa gttgccgagc gaatgggaac aaaagcgacc gcgatcacca gaatggaaag
     7801 taatctcgca tcaggtatca gcggcccatc atttgccaca ctaaaaaaat tcgcccgcgc
     7861 tactggaaaa aaactccaga tccgcttcgt ttaaccacac cgcgccgtca ttctggcggc
     7921 gcaccggata aatagtcatt tcctcgcacg atgtcttagc caccggatat cccacaggtg
     7981 agccgtgtag ttgaaggttt ttacgtcaga ttcttttggg attggcttgc gtttatttct
     8041 ggagcgtttc gttggaaggt atttgcagtt ttcgcagatg atgtcggtga aacttcgtcg
     8101 atgtcgcctc attcatacct cctgtcggta aatctgacac cctgaccaat agcccaggct
     8161 gttgtgtact cgatcagact tgccatgcgc ttcacgctca tctgcgcgct gctttcgcga
     8221 atgttgacgc attctccttc aaggccgggc aaaacatcag cttcctgttt tgttgccact
     8281 gcatgaccgc tgatcaacaa aaccttccat tgttctggtt ttaaccattt gccgcaccat
     8341 tgaacctgac gagcgatatc cgccagcatc gcgtgaaatt ttgcgttctg gtcaagattt
     8401 cgcttgtagt cagtaatgcg gatggtgact ggcttgtctt tatcgagagg agttgagagg
     8461 atggcattta ttgcggcttg ctgttgttgc ttagttcgga ggaagattgt ttgcttcatc
     8521 gaaaattctt ctctttaatt ccagcggctc tgatagcttt cattactgca attaccgttt
     8581 tgtccttccc atcctcatgc cccatcgcat aagcaccttc ttcaccatct ttccaaaagt
     8641 cgtcattcga ttcgggccag tcgatatcca gttcaatagc tgctcgcgat gcctgccata
     8701 tcacccaagc aaactctttt aattcatcgt ctcctgtgaa ctggcttttg tcttttgacc
     8761 accagttttc aaactgtcgg tagctatcgt tcacttccct ctcccccaaa taaaaaggcc
     8821 tgcgattacc agcaggcctg ctattagctc agtgatgtag atggtcattt aatactccgt
     8881 cacgttttcc tgtcgccacg cctcgtcata ttccgatttc ggcatattgg cgatgtagct
     8941 atatggcgat cctgattcaa gttgcaggaa ctggtgcgat tgctcgtcaa ggaacaacgg
     9001 aacaccacct tcccaacctt cgccgttacg ttgtttttca agcatcaaaa cagatgccgg
     9061 agatgccagt agctgttcgt ccttctctga catcttttca ccactctgaa ctctctgtaa
     9121 cgctctctcg cgagccttgt tacgccagat gatgaaaagt ttgtctgtca ggcctgttat
     9181 cgctccagag ccttttacgt ccattttccc ggttggtttt tcttcgctgt ctccttttcg
     9241 cgagtgagta acgagaatga cgtgggagtt tgttttgttt ttgaagtcgc aaatcgagtc
     9301 aacaaacgcc ttctgcccgt tatagtcatc gtcgcctatg ccacatttca tcaggctgtc
     9361 gatgatgaat aactggatcc cgtatcggcg gcgagcgtag tcgaatattt cgatcagcct
     9421 gtcggctttc gccgttccag tcaggccaaa cacccaaagt ctttcgtcat aaaatttaaa
     9481 tgcagagtca atttccagca ctggcggcat cttgcagcac gttgcctgac gggtaagtcg
     9541 cttaaggaga ataccaggct tcagctcaag tgacgcgatg cacgtcttca caccctgacg
     9601 cattgcctca agtgccatat gcccgacaac ctcggtgtta tgcgtcgcca catatcccct
     9661 ggttacatac aggtggcgag ggtgatcgac cataatacac aagcattctg cattgccgat
     9721 tttttcaaca ttcctgacga ataccccaca gccctcgaat cgatgcgcag ttaatctgcc
     9781 attgagtcgt ggtgaacgga tggcctccct gatttctctt gtcagtctga ttcttgcctc
     9841 gtatgaatcc atcccatgcc gcttatcgtc cttgtatgtg tatgtcacgc cagttttaac
     9901 tcgcgtccgg catgacccgc cgagtgagtt aaccagttga acaaccccat t
//
LOCUS       DAJTRB010000130         9937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTRB010000130 DAJTRB010000000
VERSION     DAJTRB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.130"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..218)
                     /gene="yefM"
                     /locus_tag="OO330_003660"
     CDS             complement(<1..218)
                     /gene="yefM"
                     /locus_tag="OO330_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5884374.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACALMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKS"
     gene            501..542
                     /gene="hisL"
                     /locus_tag="OO330_003661"
     CDS             501..542
                     /gene="hisL"
                     /locus_tag="OO330_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5884375.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    518..641
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            688..1587
                     /gene="hisG"
                     /locus_tag="OO330_003662"
     CDS             688..1587
                     /gene="hisG"
                     /locus_tag="OO330_003662"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5884376.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1593..2897
                     /gene="hisD"
                     /locus_tag="OO330_003663"
     CDS             1593..2897
                     /gene="hisD"
                     /locus_tag="OO330_003663"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5884377.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2894..3964
                     /gene="hisC"
                     /locus_tag="OO330_003664"
     CDS             2894..3964
                     /gene="hisC"
                     /locus_tag="OO330_003664"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5884378.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3964..5031
                     /gene="hisB"
                     /locus_tag="OO330_003665"
     CDS             3964..5031
                     /gene="hisB"
                     /locus_tag="OO330_003665"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5884379.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5031..5621
                     /gene="hisH"
                     /locus_tag="OO330_003666"
     CDS             5031..5621
                     /gene="hisH"
                     /locus_tag="OO330_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5884380.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5621..6358
                     /gene="hisA"
                     /locus_tag="OO330_003667"
     CDS             5621..6358
                     /gene="hisA"
                     /locus_tag="OO330_003667"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5884381.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6340..7116
                     /gene="hisF"
                     /locus_tag="OO330_003668"
     CDS             6340..7116
                     /gene="hisF"
                     /locus_tag="OO330_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5884382.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7110..7721
                     /locus_tag="OO330_003669"
     CDS             7110..7721
                     /locus_tag="OO330_003669"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5884383.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7907..8884)
                     /gene="wzzB"
                     /locus_tag="OO330_003670"
     CDS             complement(7907..8884)
                     /gene="wzzB"
                     /locus_tag="OO330_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5884384.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9030..9937)
                     /locus_tag="OO330_003671"
                     /pseudo
     CDS             complement(9030..9937)
                     /locus_tag="OO330_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
BASE COUNT         2455 a         2573 c         2591 g         2318 t
ORIGIN      
        1 gatttcaggc tatcgattga gtccatcaat ctccgggcgt tagcggggga acgcagtaga
       61 taagccgtct cttccagcga attgtattct tcgagtgaca tcagagcaca agcctctcca
      121 ttctgacgag taatgaggat cggggcatga tcttcaacgg ctttcatcat tgttgccgac
      181 aaattctgac gcgcttcgct gtagctaatt gtacgcatgt aaatctccta ttttgtacag
      241 ttcattgtac aatgataggt gttaattaac tatttattaa ttagtttgta gatcaagaca
      301 ttgccagcga gacgaaaaat cagactttgc tctttttggt gctgtcaagt tagaggacag
      361 tcctcttagc cccctccttt ccccgctcat tcattaaaca aatccatcgc cataaaatat
      421 ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg ttaaaagaca tcagttgaat
      481 aaacattcac agagattttt atgacacgcg ttcaatttaa acaccaccat catcctgact
      541 agtctttcag gcgatgtgtg ctggaagaca ttcagatctt ccagtggtgc atgaacgcat
      601 gagaaagccc ccggaagatc accttccggg ggctttttta ttgcgcggtt gttaacggtt
      661 cagacaggtt taaagaggaa taagaaaatg acagacaaca ctcgtttacg catagctatg
      721 cagaaatccg gccgtttaag tgatgactca cgcgaattac tggcgcgctg tggcattaaa
      781 atcaatcttc acacccagcg cctgatcgcg atggcagaaa acatgccgat tgatattctg
      841 cgcgtgcgtg acgatgacat tccctgtctg gtaatggacg gcgtggtaga ccttgggatt
      901 atcggcgaaa acgtgctgga agaagagctg cttaaccgcc gcgcccaggg tgaagatccg
      961 cgctacttta ccctgcgtcg tctggatttc ggcggctgcc gcctttcgct ggcaacgccg
     1021 gttgatgaag cctgggacgg tccgctctcc ttaaacggta aacgtatcgc cacctcttat
     1081 cctcacctgc tcaagcgtta tctcgaccag aaaggtatct cttttaaatc ctgcttactg
     1141 aacggttctg tcgaagttgc gccgcgtgcc gggctggcgg atgcgatttg cgatctggtt
     1201 tccaccggtg ccacgctgga agctaacggc ctgcgcgaag tcgaagttat ctaccgctcg
     1261 aaagcctgcc tgatccagcg cgatggcgaa atggaagaat ctaaacagca actgatcgac
     1321 aagctactga cccgtattca gggtgtgatc caggcgcgcg aatcaaaata catcatgatg
     1381 cacgcaccga ccgagcgcct ggatgaagtc atcgccctgc tgccaggtgc cgaacgccca
     1441 accattctgc cgctggcggg tgatcaacag cgcgtagcga tgcacatggt gagtagcgaa
     1501 accctgttct gggaaacgat ggaaaaactg aaagcactgg gtgccagttc aatcctggtc
     1561 ctgccgattg agaagatgat ggagtgatcg ccatgagctt taacacaatc attgactgga
     1621 atagctgtac tgcggagcaa caacgccagc tgttaatgcg cccggcgatc tccgcttctg
     1681 aaagcattac ccgcaccgtt aacgatattc tcgataacgt gaaaacgcgt ggcgatgagg
     1741 ccctgcgtga atacagcgcg aagtttgata aaaccacggt taccgcgctg aaggtgtctg
     1801 ctgatgagat cgccgccgcc agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg
     1861 tggcagtaaa gagtattgaa accttccaca ctgcgcaaaa actgccgccg gtagatgtag
     1921 aaacgcagct aggcgtgcgt tgccagcagg tcacgcgccc ggtggcttca gtgggtttgt
     1981 atattccggg cggctccgcc ccgctcttct caacggtatt aatgctggca actccggcgc
     2041 gtattgcggg ctgtaaaaaa gtggtgctgt gctcaccgcc gccgattgcc gatgaaattc
     2101 tttatgcggc gcagctgtgc gatgtgcagg acgtgtttaa cgtcggcggc gcacaggcca
     2161 ttgccgcgct ggcgtttggt acggaatctg tgccgaaagt ggacaaaatc ttcgggccgg
     2221 gtaacgcctt tgtcaccgaa gcaaaacgcc aggtaagcca gcgtctggac ggtgcggcga
     2281 tcgatatgcc cgcaggcccg tcggaagtgc tggtgattgc tgacagcggc gctacgccgg
     2341 attttgtggc ttctgatttg ctttctcagg ctgaacacgg ccctgactca caggtaattt
     2401 tactgacccc cgatgctgat atggcgcgtc gcgttgccga ggccgtcgaa cgccaactgg
     2461 cagaactgcc gcgtgccgaa accacccgcc aggcactgaa cgccagccgc ctgatcgtga
     2521 ctaaagattt agcgcagtgc gtggagatct ccaaccagta cggcccggag cacctgatca
     2581 ttcagacccg caacgcccgc gaactggtcg atggcatcac cagcgccggt tcggtatttc
     2641 ttggtgactg gtcaccggaa tcggcaggcg actacgcctc cggcaccaac cacgtgctgc
     2701 cgacttacgg ttacaccgcc acctgttcca gcctcgggct ggcagatttc cagaaacgca
     2761 tgaccgtaca ggaactgtcg aaagaaggtt tctccgcgct ggcttcgacc attgaaacac
     2821 tggccgccgc cgagcgcctg accgcccaca aaaatgccgt tactttgcgt gttaacgtcc
     2881 ttaaggagca agcatgagca ccgtgactat tactgattta gcgcgtgaaa acgtccgcaa
     2941 cctgacaccg tatcagtcgg cgcgtcgtct gggcggtaac ggcgacgtct ggctgaacgc
     3001 caacgaatat cccacagccg tggagtttaa gcttactcag caaacgctca accgctaccc
     3061 ggaatgccag ccaaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga
     3121 acaggtgctg gtcagccgtg gcgcggacga aggtattgaa ctgctgatcc gcgctttttg
     3181 cgaaccgggt aaagacgcca tcctctactg cccgccaacg tacggcatgt acagcgtcag
     3241 cgctgaaacc attggtgtcg aatgccgcac agtgccgacg ctggaaaact ggcaactgga
     3301 cttacagggc atttccgaca agctggacgg cgtaaaagtg gtctatgttt gcagccccaa
     3361 caaccccacc gggcaactga tcaatccgca ggattttcgc accctgctgg agctaacccg
     3421 cggtaaggcg attgtggttg ccgatgaagc ttatatcgag ttttgcccgc aggcatcgtt
     3481 agctggctgg ctggcggaat atccgcacct ggcgattttg cgtacccttt cgaaagcctt
     3541 cgcgttggcg gggcttcgtt gcggatttac gctggcaaac gaagaagtca tcaacctgct
     3601 gatgaaagtg atccccccct acccgctctc gacgccggtt gccgacattg ccgcacaggc
     3661 gttaagcccg cagggaatcg tcgccatgag cgaacgggtg gcgcaaatta ttacagaacg
     3721 cgaatacctg attgccgcac tgaaagagat cccctgcgtg gagcaggttt tcgactctga
     3781 aaccaactac attctggcgc gctttaaagc ctccagtgca gtgtttaaat ctttgtggga
     3841 tcagggcatt atcttacgtg atcagaataa acaaccctct ttaagcggct gcctgcgaat
     3901 taccgtcgga acccgtgaag aaagccagcg cgtcattgac gccttacgtg cggagcaagt
     3961 ttgatgagtc agaagtatct ttttatcgat cgcgatggaa ccctgattag cgaaccgccg
     4021 agtgattttc aggtggaccg ttttgacaaa ctcgcctttg aaccgggcgt gatcccggaa
     4081 ttgctgaagc tgcaaaaagc gggctacaag ctggtgatga tcactaatca ggatggtctg
     4141 ggaacacaaa gtttcccgca agcggatttt gatggcccgc acaacctgat gatgcagatc
     4201 ttcacctcgc aaggcgtgca gtttgatgaa gtgctgattt gtccgcacct gcccgccgat
     4261 gagtgcgact gccgtaagcc gaaagtaaaa ctggtggagg gttatctggc tgagcaagcg
     4321 atggatcgtg ccaacagtta tgtaattggc gatcgcgcga ccgacattca actggcggaa
     4381 aacatgggca ttaatggttt acgctacgac cgcgaaaccc tgaactggcc aatgattggc
     4441 gagcaactca ctaaacgaga ccgttacgcc catgtagtgc gcaacaccaa agagacgcag
     4501 attgacgtcc aggtgtggct ggatcgcgaa ggtggcagca agattaacac cggcgttggc
     4561 ttctttgatc acatgctgga tcagatcgcc acccacggcg gtttccgcat ggaaatcaat
     4621 gtcaaaggcg acctctatat cgacgatcac cacaccgtcg aagataccgg cctggcgctg
     4681 ggcgaagcgt taaaaattgc cctcggcgac aaacgcggta tttgccgctt tggttttgtg
     4741 ctgccgatgg acgaatgcct tgcccgctgc gcactggata tctctggtcg cccgcacctg
     4801 gaatataaag ccgagtttac ctaccagcgt gtgggcgatc tcagcactga aatgatcgaa
     4861 cacttcttcc gttcgctctc ttacaccatg ggcgtgacac tacacctgaa aaccaaaggt
     4921 aaaaacgatc atcaccgtgt agaaagcctg ttcaaagcct ttggtcgtac cctgcgccag
     4981 gccatccgcg tcgaaggcga caccctgccc tcgtcgaaag gagtgctgta atgaacgtgg
     5041 tgatccttga tactggctgc gccaacctga actctgtgaa gtctgccatt gcgcgccacg
     5101 gttatgaacc caaagtcagc cgtgacccgg acattgtgtt gctggccgat aaactgtttt
     5161 tacccggcgt cggcaccgca caagcagcga tggatcaggt gcgtgagcgc gagctgtttg
     5221 atctcatcaa agcctgtacc caaccggtgc tgggcatctg cttagggatg caactcctgg
     5281 gacgacgcag cgaagagagc aatggcgtcg acttgctggg catcatcgac gaagacgtgc
     5341 cgaaaatgac cgactttggt ctgccactgc cacatatggg ctggaaccgc gtttatccgc
     5401 aggcaggcaa ccgcctgttt caggggattg aagacggcgc gtacttttac tttgttcaca
     5461 gctacgcaat gccagtcaat ccgtggacca tcgcccagtg taattacggc gaaccgttca
     5521 ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt ccacccggag cgttctggtg
     5581 ccgctggcgc taagttgctg aaaaacttcc tggagatgtg atgattattc cggcattaga
     5641 tttaatcgac ggcaccgtgg tgcgtctcca tcagggcgat tacggcaaac aacgcgatta
     5701 cggtaacgac ccgctgccgc gcttacagga ttacgctgcg caaggtgccg aagtgttgca
     5761 cctggtggat ctgaccgggg caaaagatcc ggctaagcgt caaatcccgc tgattaaaac
     5821 cctggtcgcg ggcgttaacg tcccggtgca ggttggcggc ggtgttcgta gtgaagaaga
     5881 cgtggcggcg ttactggaag ctggcgttgc gcgcgtggtg gtcggttcca ccgcagtgaa
     5941 atcaccggac gtagtgaaag gctggtttga acgtttcggt gccgatgcgc tggtgctggc
     6001 actggatgtc cgtattgacg agcaaggcaa caagcaagtg gcagtcagcg gctggcaaga
     6061 gaactcgggc gtttcactgg aacaactggt ggaaacctat ctgcccgtcg gcctgaaaca
     6121 tgtgttgtgt accgatatct cgcgcgacgg cacgctggcg ggatctaacg tctctttata
     6181 tgaagaagtg tgtgccagat atccgcaagt ggcgtttcag tcctccggcg gcattggcga
     6241 cattaacgat gttgctgccc tacgtggcac tggcgtgcgc ggcgtaatag ttggtcgtgc
     6301 attactggaa ggtaaattca ccgtgaagga ggccatcgca tgctggcaaa acgcataatc
     6361 ccgtgtctcg acgttcgtga tggtcaggtg gtgaaaggcg tacagtttcg caaccatgaa
     6421 atcattggcg atatcgtgcc actggcaaaa cgctacgccg aagaaggtgc agatgaactg
     6481 gtgttctacg atatcaccgc ttccagcgat ggccgtgtgg tagataaaag ctgggtatct
     6541 cgcgtggcgg aagtgatcga cattccgttt tgtgtggcgg gtgggattaa gtctctggaa
     6601 gatgccgcga aaattctttc ctttggcgcg gataaaattt ccatcaactc ccctgcgctg
     6661 gcagacccaa cattaattac tcgcctggcc gatcgctttg gcgtgcagtg tattgtggtt
     6721 ggtattgata cctggtacga cgccgaaacc ggtaaatatc atgtaaatca atataccggc
     6781 gatgaaagcc gcacccgcgt cactcaatgg gaaacgctcg actgggtaga ggaggtgcaa
     6841 aaacgcggcg ccggagaaat cgtcctcaac atgatgaatc aggacggcgt gtgtaacggt
     6901 tacgacctca agcagctgaa aaaagtgcgt gaagtttgcc acgtcccgct gattgcctcc
     6961 ggtggcgcgg gcaccatgga acacttcctg gaagccttcc gcgatgccga cgttgacggt
     7021 gcgctggcag cttccgtatt ccacaaacaa ataatcaata ttggcgaatt aaaagcgtac
     7081 ctggcaacac agggcgtgga gatcaggata tgttaacaga acaacaacgt cgcgaactgg
     7141 actgggaaaa aaccgacggt ctgatgccgg tgattgtgca acacgcggta tctggcgaag
     7201 tgttaatgct gggctacatg aacccggaag ccttagacaa aaccatcgaa agcggtaaag
     7261 tcaccttctt ctcgcgcact aaacagcgac tgtggaccaa aggcgaaacc tcgggcaatt
     7321 ttctcaatgt ggtaagtatt gccccggact gcgacaacga cacattactg gtgctggcga
     7381 atcccatcgg gccaacctgc cacaaaggca ccagtagctg cttcggcaac accgctcacc
     7441 agtggctgtt cctgtatcaa ctggaacaac tgctcgccga acgtaaatat gccgacccgg
     7501 aaacctccta caccgccaaa ctgtatgcca gcggcactaa acgcattgcg cagaaagtgg
     7561 gcgaagaagg cgtggaaacc gcactggcag caacggtaca tgatcgcttt gagctgacca
     7621 acgaggcatc tgacttgatg tatcacctgc tggtgctgtt gcaggatcag gatctggatt
     7681 taacgacagt aattgagaac ctgcataaac ggcatcagtg agttacgggc tgagcaggtg
     7741 tgatgttgtt gtcacacccg gagccattgc ccgatgcgca aacttatcgt gcctacacat
     7801 tatctcaaca cacgtaggcc ggataagatg cgccagcatc gcatccggca aaaaaaacgg
     7861 gcatggtgtc accaccctgc ccgtttctct taaatgcaca ataatattac ttcgcgttgt
     7921 aattacgcag agcgttacgc cccaacacaa tccccgcgcc aaccatgccg cccagaagca
     7981 ctgccagaat caaggtgatt gcctttttcg gactatcgcg acgaataggt aacgtcggtt
     8041 tcatcacata gcggtaagca tgaatatcaa gatcatcaaa ctttaatttt tcaatatcca
     8101 acaggttttg acgtgtctga tagtagtttg gtgagaacac caacggacga gtcgcttcat
     8161 gcttaatcat cgactccagc gcttcgctcc ctagaaggaa caacgtatct tgcgtcacat
     8221 cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
     8281 cctgaatctg acggatacgc agatctttct gctcctgcgc gaccacttcc tgggttctta
     8341 aagagtcctg caagtttttc cgtcccagag caatgttgtc cttgagatcc ttttctagct
     8401 cttgattcac tttatcatca acttgctgaa tgtattgggc caacttcatt tgtgcgccct
     8461 ctgcagtttg cccaacataa gaaacagtca atggtaattg ctggttctta acagaaggtt
     8521 cgatggtaag tttttctggc tcttcctgat tatccagcgt ttctgctaat gcagagaagg
     8581 cagaactgaa gcgaccaatt aacgtctcct gcaaatccga tactttcggt gcagcctgac
     8641 catagataac attcatggca ttgttatagc cagcaatttg ccccacgtcg ggctgagtga
     8701 taattgctgt tgacgtccat ttctccttcg ctactgccaa ataaccaata gccagggcaa
     8761 tagccacaat gacggaaatg ataattgtca tcttgccacg ccacaactgc actagtaaat
     8821 caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta ttttctactc
     8881 tcatcattac cctaactgac ggaaatttcc aagtccgcat agtttagcga taatccccgt
     8941 gaatgctata ggataagtga taaaaataat gaattattgc ggaggtaaca aaagacaatc
     9001 agggcgtaaa tagccctgat aacaagatgt tagtcgctgc caaagagatc gcgggtgtag
     9061 accttatctg ccacatcctt aagctcttct gccatacggt tggagataat gacgtcggct
     9121 tgttgtttga aggtggcgag atcacgttcc aggcgagagt tgaagaatga gtcttctttc
     9181 atcaccggct cgtagatgat cacttcaacg cctttcgcct tgatacgctt cataatcccc
     9241 tgaatagaag acgcacggaa gttatctgac ccgctcttca taatcagacg ataaataccc
     9301 accacttgcg gtttgcatcg acaattgccg agatcaggtt attcggcaca gactggtagt
     9361 tcgccagtaa ctgcttggta tctttcggca gacaataacc accataacca aacgacggat
     9421 tgttgtaatg gttgccaata cgcggatcga gacaaacgcc ttcgataatc tggcgagtat
     9481 tcagacctaa actttctgca taactatcca gttcattaaa gtatgctacg cgcatcgcca
     9541 gataggtatt agcgaaaagt ttaatcgctt ctgcttcagt ggagtcggta aacagggtcg
     9601 ggatattttg cttaatcgcg ccttcctgta acaacgcagc aaaacgttct gcgcgttctg
     9661 aacgctcacc gatgacaata cgtgacggat ggagattatc gtaaagggct ttaccttcgc
     9721 ggaggaattc cggagagaaa ataatatttt cagtacgata tttcttatgc atcgctgcgg
     9781 taaaaccaac gggaaccgtt gatttgatga ccataaccgc ataaggattt atctcaacta
     9841 cgtctttaat tactgattct acactggatg tattgaaata attagtttta ggatcatagt
     9901 cggttggagt ggcgatgatg acataatcag catcccg
//
LOCUS       DAJTRB010000131         9931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTRB010000131 DAJTRB010000000
VERSION     DAJTRB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.131"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..715
                     /locus_tag="OO330_003672"
                     /pseudo
     CDS             1..715
                     /locus_tag="OO330_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            728..1641
                     /locus_tag="OO330_003673"
                     /pseudo
     CDS             728..1641
                     /locus_tag="OO330_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2320..2538
                     /locus_tag="OO330_003674"
     CDS             2320..2538
                     /locus_tag="OO330_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5884385.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2756..2998
                     /locus_tag="OO330_003675"
     CDS             2756..2998
                     /locus_tag="OO330_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884386.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="OO330_003676"
     CDS             complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="OO330_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5884387.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="OO330_003677"
     CDS             complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="OO330_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5884388.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="OO330_003678"
     CDS             complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="OO330_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5884389.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="OO330_003679"
     CDS             complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="OO330_003679"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8264..8266),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5884390.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9045..9878
                     /gene="fdhD"
                     /locus_tag="OO330_003680"
     CDS             9045..9878
                     /gene="fdhD"
                     /locus_tag="OO330_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5884391.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2396 c         2545 g         2500 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa accaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
      901 accgcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
      961 gcctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     1021 atggcgacag gtgtgggctt ggcattaatg tatgcaacat catcaggacc gtattatgcg
     1081 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc tgaattgttt
     1141 aaccagtcgg atatttctgt gcgcaacaaa tatagcccgg cttttgataa atggaatgaa
     1201 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     1261 ttagcgacgg cgataaatga aactcttgag ggggatacga atgttgccgt tgtgatgggg
     1321 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     1381 ctccttgaaa aacgcctcgc cgatagtgga gaaatagaaa atctttttga tcaacaggga
     1441 tattttagtt gcgattcatt aacacccgaa gaattaatga tgttaaaaga cctttttgat
     1501 attcgagttc ttcatgtctc taacaaattc aatgttggat tgcaaggtaa tatcaacact
     1561 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaata taactcgtat
     1621 accgtaggga tgggagtctg acgagtaaaa ctcattagga ttatcagaat tcggacaatg
     1681 cattgcaaaa atgacataca aagagagatg tgtgcaggta cttagccgcg aggggcatgg
     1741 cgaagccata cagaaagtac cagtattcag aactccagtg aaagcggcat agtggccgcc
     1801 tgctcaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct atgatgcgat
     1861 taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga tgccgggggt
     1921 gttccggaag gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa
     1981 gtaaagaccg gttggtggca gtactgaccg gtctgtgatg aaagattaat ttcaccttaa
     2041 atcctggtgc tgttaggatg ctgacgtttt tcggctacac cattcagatt atgcaaataa
     2101 atatccctga atataatata ttcatagcac ccataactca tcctgggcga taactaaacc
     2161 atttttgagt aataaatgaa acaactgcaa cgctcttatt atttctggaa aacgcctcgc
     2221 aaaaacaaaa tgacatgcaa aaatccgcat ttttttaaat gcttttccgg cgtaattttc
     2281 gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa tacggttttt
     2341 cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggctg gcgtaaggtt
     2401 tcacgatctg caattttacg cgaagcggtt gagcaatatc tggagcggca gcaatttccg
     2461 gtgcgtaaag caaagggagg caggcaaaag ggggaggtag ttggtgttga tgatcagtgt
     2521 aaggagcata aggaatgaaa aaaatttttc attcttgaat ataaaaaaca gatgccctta
     2581 ttctggtatt aatacaaggc tgttttactt gaacttataa taactgcaac tgttacatcg
     2641 tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt ctttaaagat
     2701 tcggcaaggg tacttttctc tccatagatt caaaaatgat attggcgaga tatttatgaa
     2761 ctctctggca ggtatagata tgggcagaat tttactcgat ttatcgaatg aggtgattaa
     2821 gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt gcggacctat taagggaagc
     2881 ggtagatcaa tacctgataa atcaatcgca aacagcaaga accagtgttc ctggcatctg
     2941 gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg
     3001 ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
     3061 gtgaacgcct tatccggcct acaaagtcat gcaaattcaa tatattgaag tttattggta
     3121 ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt catcagtgac atacccataa
     3181 ccttatcgat gtgttggagg gaagaacgag gcaaacagac aattgcggca tgatcttgaa
     3241 tgggtaacgc ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga
     3301 cctgcgccac ccggaaaacg tcagagatta ctctccttca cccggaaaca ggaacgggtt
     3361 aatggaacta cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag
     3421 gtcatctgcc acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt
     3481 gccgcagtca tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta
     3541 atgcagtttg ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt
     3601 ttcacacagg ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct
     3661 ggacaccggc atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg
     3721 ggctttgccg gggatcagat tggccatctg cgcccagtag agcgacagcg cagcccagat
     3781 aaacggtgct ttatcgctgc tgacggacga gaaatcagag gcaaacagcg cgctggccat
     3841 atcttccagc tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc
     3901 gctcatttca ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc
     3961 acgcggcaga acgtgaatat ccagcggggg cttgccctgt gcgctggctt ctttaatgcg
     4021 cgcggtcaga tccatctcca gcggatggtc gtacagcacc actttctggg cgtgggcgat
     4081 aagcgcagca aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg
     4141 ctcggcgcgg cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc
     4201 cgccgtacgt ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca
     4261 gctttctttt ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc
     4321 caggcgctgg taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg
     4381 gcgtagatat gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt
     4441 aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc
     4501 acgccgctca ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga
     4561 ccgaagttat aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc
     4621 ttcgcccaaa agatatcatc ccgattgatt aggttttgat gccagtaacg gaaaaacatg
     4681 atgatgaacg aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc
     4741 ggtgtgccca tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc
     4801 accgccgcca ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg
     4861 tagcgcacga tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat
     4921 tattctcttc ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg
     4981 caaaggtagc cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca
     5041 cggtttcgct gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat
     5101 ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc cggatcgtac agacccgcat
     5161 tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct cgccagcgtt ttcatcgact
     5221 ctttcgtgcc gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc
     5281 caaccaccac gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga
     5341 ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg
     5401 actggaagtc gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt
     5461 tcaggcagcc tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt
     5521 tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca cgatttggcg cttaaatcat
     5581 tggggttgtc gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg
     5641 aacacgccac ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca
     5701 cttcttcctg gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac
     5761 gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca
     5821 caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat ggcgttaaag
     5881 tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc ggaataccga
     5941 tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt
     6001 tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa
     6061 gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa atcgcgttca
     6121 acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat
     6181 acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagctg
     6241 ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt
     6301 agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt
     6361 cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa
     6421 tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg
     6481 ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg
     6541 cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca
     6601 tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac
     6661 cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac
     6721 cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt
     6781 ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tgatccgggt
     6841 tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct
     6901 caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc
     6961 gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca
     7021 cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt
     7081 tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc gacagacaac
     7141 cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc
     7201 cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag cctttatccc
     7261 acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg
     7321 ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca
     7381 gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg
     7441 gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc agcccctgaa
     7501 tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata ttgccgagca
     7561 gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc
     7621 cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc
     7681 agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt
     7741 agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat
     7801 cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc
     7861 gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga
     7921 tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca ttgttcagca
     7981 ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt
     8041 agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt
     8101 tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac
     8161 cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac
     8221 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt
     8281 tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt
     8341 tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt
     8401 cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat
     8461 caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg
     8521 gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa
     8581 gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg
     8641 cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg
     8701 tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg
     8761 ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga
     8821 tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg
     8881 tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag
     8941 gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt ttgtaacaga
     9001 ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa aaaacacagc
     9061 gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc
     9121 gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg
     9181 tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg
     9241 cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg
     9301 atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta
     9361 tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg
     9421 tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg
     9481 atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca gtggggcgac
     9541 tgactggttg tactcacgcc gctgcctgga tgttgccatc tggcgaactg gtcggcgggc
     9601 atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag
     9661 gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc
     9721 aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg
     9781 ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg
     9841 caacggttta tacccatccg cagcgtttga gcaattaatt aaaataagtc ctgcgatata
     9901 tattaaattg caggacttat tcatttcgtg a
//
LOCUS       DAJTRB010000132         9895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTRB010000132 DAJTRB010000000
VERSION     DAJTRB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.132"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO330_003681"
     CDS             complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO330_003681"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5884392.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO330_003682"
     CDS             complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO330_003682"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5884393.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO330_003683"
     CDS             complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO330_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5884394.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4682..6442)
                     /locus_tag="OO330_003684"
     CDS             complement(4682..6442)
                     /locus_tag="OO330_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCR5884395.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSKWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6572..7198
                     /locus_tag="OO330_003685"
                     /pseudo
     CDS             6572..7198
                     /locus_tag="OO330_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7397..8485
                     /gene="serC"
                     /locus_tag="OO330_003686"
     CDS             7397..8485
                     /gene="serC"
                     /locus_tag="OO330_003686"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5884396.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVSSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8556..9839
                     /gene="aroA"
                     /locus_tag="OO330_003687"
     CDS             8556..9839
                     /gene="aroA"
                     /locus_tag="OO330_003687"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5884397.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2363 c         2477 g         2534 t
ORIGIN      
        1 aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat atggtagatg
       61 cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa aaagcggcaa
      121 ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc gtactgctca
      181 agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc atcgagtttg
      241 tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg gagaagttcg
      301 attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc cgaatcgtca
      361 tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt tttgttcgcc
      421 agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc
      481 tcgtcgttca tctgtttgag atcgagcatt actaggtcgg ttacttccag cagttcatca
      541 atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg aatgccttct
      601 tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc ttcaccgccg
      661 gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacttccttc
      721 atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga
      781 caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acctgggccg
      841 tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt
      901 ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct
      961 gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag
     1021 gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta
     1081 attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg
     1141 agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt
     1201 tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac
     1261 cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac
     1321 gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat
     1381 ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca gcacctttct
     1441 ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt
     1501 ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc agaacagact
     1561 gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta
     1621 ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc gggtattcgc
     1681 cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat
     1741 atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg
     1801 tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt
     1861 gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt
     1921 gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc ggttcagact
     1981 tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg
     2041 ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc
     2101 ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt
     2161 tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc ttgaagttca
     2221 gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc cccatagtgt
     2281 acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca tcgaggccca
     2341 taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca tcgtattccg
     2401 gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg
     2461 cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg
     2521 tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc
     2581 cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc
     2641 cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt
     2701 cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc tgtagagagg
     2761 tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt
     2821 agtcaccgat gatacggcca cggccataag catctggcag accggtcaga acaccggatt
     2881 tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac
     2941 ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac gctttgcagg
     3001 aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag
     3061 tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg tgagaggtga
     3121 tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt tccagtttaa
     3181 caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg
     3241 actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg tttacttcat
     3301 tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg
     3361 acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt
     3421 aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac
     3481 cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag ttcatcacgg
     3541 tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc ggagatgcga
     3601 agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac
     3661 cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc
     3721 ggccagaata actcatccat actgccagac ataccatcag gtttgccagg ataccaagac
     3781 agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc
     3841 cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca
     3901 gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac tgacaccagg
     3961 tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag agatcggctc
     4021 cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc agttttgcca
     4081 tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg
     4141 agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt
     4201 tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa agatcaaaag
     4261 ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag
     4321 cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg
     4381 catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa taaattttaa
     4441 aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta attaaaaggg
     4501 agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa ttaccgatat
     4561 ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg ggcattaaaa
     4621 aaacgccccg taatataact cagactaatc aaatagccta cattgcgtag gctatttgat
     4681 tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacaacg
     4741 actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg taaaacgccg
     4801 cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac atgcgaacaa
     4861 acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat aacaacgttt
     4921 gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta aactccatcg
     4981 tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt
     5041 ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca
     5101 gctcacgcac gcgggtaaag tcatcaaaat cttcttcatc cagttgctcg atgaggttca
     5161 ggtaatcttc tttttcccac ttgctgcctg gtagcgaaag aatcgtttca cgtaaatggc
     5221 tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag
     5281 gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata taaacttctt
     5341 tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg gtgccggaga
     5401 aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac
     5461 cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga
     5521 aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc agctcggtca
     5581 cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc
     5641 cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca agggaaccat
     5701 catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca accaccgctg
     5761 ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc
     5821 ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttgcggg
     5881 tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc atcggaatat
     5941 aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca cgatcttcgt
     6001 taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga tcgtaaaatg
     6061 cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc agtgggaacc
     6121 atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc agccagaaat
     6181 cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg
     6241 ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa
     6301 tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc
     6361 cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg gcatctttgc
     6421 cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata
     6481 cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt
     6541 ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc accgtatggc
     6601 atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt
     6661 tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca gatgtcgctg
     6721 tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc gttttatgat
     6781 gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt ataccgcctg
     6841 gtaatatgat ggcgagctgg cctggtcgaa actcaataac tccaacatga cggaatttga
     6901 gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac gtctggcgat
     6961 tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg tgaaaccggg
     7021 tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg agtcggcgga
     7081 ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa aacaattatg
     7141 tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg tgacctgaca
     7201 gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat gcaagccaca
     7261 tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata aaaccgatag
     7321 ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta atagcaacgc
     7381 aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg caatgctacc
     7441 ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc ttggtacgtc
     7501 ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg aagccgagaa
     7561 ggattttcgc gatcttctta atgtctcctc caactacaag gtattattct gccatggcgg
     7621 tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa ccaccgcaga
     7681 ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa aatactgcac
     7741 gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta agccaatgcg
     7801 tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg aaaccatcga
     7861 cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg ccgccgactt
     7921 ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa tttacgctgg
     7981 cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg aagatttgct
     8041 gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca acgataacgg
     8101 ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg tctttaaatg
     8161 gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa aagcagaact
     8221 gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga aagctaaccg
     8281 ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat tgttccttga
     8341 agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg gcggaatgcg
     8401 cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag acttcatggt
     8461 tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac agccagcctg
     8521 tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg ttacaaccca
     8581 tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct aaccgcgctt
     8641 tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg gatagcgacg
     8701 acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg ctttcagccg
     8761 atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa ggtgccctgg
     8821 agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct ctttgtctgg
     8881 atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg attggtcatc
     8941 tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa gaaaattatc
     9001 cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat ggctccgttt
     9061 ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa gatacggtga
     9121 ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc aatctgatga
     9181 agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta aaaggcgggc
     9241 agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg gcttcttact
     9301 tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt ggacgtaaca
     9361 gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg accatttgct
     9421 ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat atggatatga
     9481 accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca aaaggcacca
     9541 ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg tttgcgatgg
     9601 caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac attcgtatca
     9661 ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac cggatggcga
     9721 tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat cccaaatgca
     9781 cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag gcagcctgaa
     9841 tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca cattg
//
LOCUS       DAJTRB010000133         9682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTRB010000133 DAJTRB010000000
VERSION     DAJTRB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.133"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            50..244
                     /locus_tag="OO330_003688"
     CDS             50..244
                     /locus_tag="OO330_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5884398.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            complement(494..1396)
                     /locus_tag="OO330_003689"
                     /pseudo
     CDS             complement(494..1396)
                     /locus_tag="OO330_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1456..1734
                     /locus_tag="OO330_003690"
     CDS             1456..1734
                     /locus_tag="OO330_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884399.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1735..2262)
                     /gene="gadE"
                     /locus_tag="OO330_003691"
     CDS             complement(1735..2262)
                     /gene="gadE"
                     /locus_tag="OO330_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5884400.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(3061..3633)
                     /gene="hdeD"
                     /locus_tag="OO330_003692"
     CDS             complement(3061..3633)
                     /gene="hdeD"
                     /locus_tag="OO330_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5884401.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3888..4220
                     /gene="hdeA"
                     /locus_tag="OO330_003693"
     CDS             3888..4220
                     /gene="hdeA"
                     /locus_tag="OO330_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5884402.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            4336..4662
                     /gene="hdeB"
                     /locus_tag="OO330_003694"
     CDS             4336..4662
                     /gene="hdeB"
                     /locus_tag="OO330_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5884403.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4726..5372
                     /locus_tag="OO330_003695"
                     /pseudo
     CDS             4726..5372
                     /locus_tag="OO330_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5414..5944)
                     /locus_tag="OO330_003696"
     CDS             complement(5414..5944)
                     /locus_tag="OO330_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5884404.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(6100..6666)
                     /gene="slp"
                     /locus_tag="OO330_003697"
     CDS             complement(6100..6666)
                     /gene="slp"
                     /locus_tag="OO330_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5884405.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6913..8136)
                     /locus_tag="OO330_003698"
     CDS             complement(6913..8136)
                     /locus_tag="OO330_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884406.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8717..>9682)
                     /locus_tag="OO330_003699"
     CDS             complement(8717..>9682)
                     /locus_tag="OO330_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5884407.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIKQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2826 a         1919 c         2030 g         2907 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agaaattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat aaatacattc tggcggctac cttcatccac taatgccgtg acatacatgc
      541 caggcagcaa gtcaccattt gggttgggga aaatcgcccg taacgtcacg gagcccgtgg
      601 tttcatcaac cgtcgggtcg gagaatttca gcgtgccggt ctggctgtaa cgtttaccat
      661 tttccagatt gagctgtact ggcgtactgc cctgaacctg tttgatttgc ccactggcga
      721 cctcttcttt catgcgtaag aaatcttgca ccgactgcgt gagatcgaca taaatcgggt
      781 ccagacgttg tacggtaacc agcgaatctg cctgattagc ggtaacgagt gcgccgacgg
      841 tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt gacattcgcg tattgcagat
      901 tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac attggcttct gcttcattca
      961 gctgggtgcg cgcggtgtcg taatcctgac ggctaacgta gttggtcttc agcaacgatg
     1021 cctggcggtt aaaggtgatg cgggcattgc tggcggtaga gagcgctttc gccagcgagc
     1081 ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc aatctgatac agcgaatcgc
     1141 cctggttcac tttatcgcct tcgataaagt tgcgtttaat gataataccg cccacctggg
     1201 gacgtatctc ggcaacttca taaggaacgg ttctaccggg caattcgctc aacacattga
     1261 ccgaaccagg ggagagtgtg acgacaccga cctcaggcgt catggcggcg gcgttttccg
     1321 ccgatttgtc atcgcaggcg gtgagcatcg cgctgcagaa taacaacggt attaacagct
     1381 ttcttcttct gttcatttta gtccctgaaa attcttgaga aataacggtt ctttaaattg
     1441 cgcgcgagga gagatatgag ggatttacag accagcggca tagtggggtt gtcagccagt
     1501 aaggtgggat acaggcacag tgatcgacat ggtgaggtca acgacaaaaa aaatgttgcc
     1561 gttctgccaa cagttcctgc cagcattcgg gcaacgaagg gttctactgg tggatacaca
     1621 taccagggga atcaagtcgt tttagcctct cctttagtaa tcaccggggg taatgaaatc
     1681 agcatctgca tccctcgtca tgccagccat caatttcagt tgcttatgtc ctgactaaaa
     1741 ataagatgtg atacccaggg tgacgatgtc gctcatacgt cttagtttca gcttgtacat
     1801 aatgttgaat ttctgaatct ttaatgtttg ttgcgaaata ttgagcgtcg acgtgatatt
     1861 gctttcattt ttatttttca gaatcagttg cataattttg aactgattat gtgaaaaaca
     1921 taattgtgaa ttcttatggg gcaagtgttt accataagta atctcaagaa gatttttgat
     1981 actttctttg cggctaacaa aataaatagg cgctttagct tttagtttac tgatgtgata
     2041 aggtgccaaa acaataattc tctcggcatc taatttctcc agaaatttaa tcgcttcttc
     2101 atcaaggata tgattgtgat aggtatcaat gataaccttg aagggtttat tgcctacttt
     2161 tttgatctct gacagggaat tgatttttat catttcgtga ttatctttca actgccaaaa
     2221 gccctgtaaa agaaaagaat ctttcgtcat gagaaaaatc ataacttgct ccttagccgt
     2281 tatcgtttcg aacatgtcat ccttttcatt atttacatcc ttgtccgaat cgttgttcaa
     2341 tatagtaaac gcctataaga actttgccct tcgtgaacta atacgtctat taacactaat
     2401 caatacttca gatttatcca tcacgcactc catatattga tcctaagtaa gattcctggt
     2461 tgttatcagc ttgtaaatag taaacaaaaa gttaacgttt gcggattact gcccaagaat
     2521 aagttatcaa taaccagttt gtgatctctg aagaatatta ctaaagttaa aatgtaatcc
     2581 gatttaaata tcgagtctcc ttgtttcgac ttaagctggc aattggattg ccagctttct
     2641 tttaacgcat tgaaattacc tgagtaaata acgcattcat gtcttcaggt ttttatatag
     2701 cgtctggaga cacggatatt tatgcaatgt aatcaacgcc agttgcaaaa aaatgaaccc
     2761 ggattactta tttaaccgcc catagcatca acgcttacgg aactctttgt tcactttccc
     2821 acagcacttt taaaacatta aacctgacta cggaaaatat cagccatgat aatgtttgaa
     2881 atggctaatt tgccatggag tgaaaaaaat tagatgaaat tcagtaggtt gaaataatca
     2941 ctagcaggta attatttcaa tgatagtgcg caattgatct acaacactgc gtagcggaga
     3001 gagtattaat cggatcatag tcacatcaag tgactatgat cagggtgaca accggggtaa
     3061 ttattgctgc ttaacgaaca aactggcgaa gctgaacagg ctggcggcgc taaatatcag
     3121 ttcaattccc accagtgtgg aaaccagcgt tacagacacc atcggcgttg caccaaggaa
     3181 tatccaggca atgacgatat ccagcacacc aataacgagc tgtagccagc tgcctttcat
     3241 tgaacgctga cgataccaac tcatcaggcg aataacccct gcaacacaga acaaaccggc
     3301 aataaatgcc gcaatggcaa aaatgcccag ctccggtgca cggatgaaga aatagccgat
     3361 caataaatag gcgactgcga cgaggaaacc ggataatacc ggccagaaat tatgactgcg
     3421 gttgctgaat aacccgacaa taagcgcaat acccgagcag attaataatg cacccactac
     3481 tgtgcttaaa atatcgccag agacgaacgg gaaactgata cacagcaacc cgacgataaa
     3541 cagcagcacg gcaataaact ggattgctct gcgatgcttt ttcagcatct ccagatcaaa
     3601 cttcaaaatt gttgccttat ctatatataa catagaacca ccctataaaa ttaagaagaa
     3661 aatcccctgc tatcaatcta tgccaaaaac gcgtctaaga atgcagtcga tttaataaaa
     3721 atttcctaat tgcagtatct gatgcatctg taactcattg tattgaaata aaaatatctg
     3781 attttgatat tttccatcaa catgacatat acagaaaacc aggttataac ctcagtgtcg
     3841 aaattgattc gtgacggctc tttcacttta tagttgagga tattacgatg aaaaaagtat
     3901 taggcgttat tcttggtggt ctgcttcttc tgccagttgt gagcaatgca gcggatgcgc
     3961 aaaaagcagc tgataacaaa aaaccggtca actcatggac ctgtgaagat ttcctggctg
     4021 tggacgaatc cttccagcca actgcagttg gttttgctga agcgctgaac aacaaagata
     4081 aaccagaaga tgcggtttta gatgttcagg gtattgcaac cgtaacccca gctatcgttc
     4141 aggcttgtac tcaggataaa caagccaact ttaaagataa agttaaaggc gaatgggaca
     4201 aaattaagaa agatatgtaa ttccgggaat gcgttacatc gtacttcctt gcatattgaa
     4261 caggccggaa tatcttcttt aaaagcagct attcctcctg ttcatatata atctctatat
     4321 tgaatgggtt acaaaatgaa tatttcatct ctccgtaaag cgtttatttt tatgggcgct
     4381 gtagcggctt tgtcactggt gaacgcacaa tctgcgctgg cagccaatga atctgctaaa
     4441 gatatgacct gccaggaatt tattgatctg aatccaaaag caatgacccc ggttgcatgg
     4501 tggatgctgc atgaagaaac agtatataaa ggtggcgata ccgttacttt aaatgaaacc
     4561 gatctcactc aaattcctaa agtgatcgaa tactgtaaga aaaacccgca gaaaaatttg
     4621 tataccttca aaaatcaagc atctaatgac ttgccgaatt aatgaggtac aagtaaaaag
     4681 gagtagcaag ttgagccatc ttgctgctcc tttttgcatt tttatatgac agcagaattt
     4741 attatacgtc ttatactcgc ggcaattgcc tgtggcgcta ttggcatgga aaggcaaatg
     4801 cgcggcaaag gagcagggtt acgcacacat gtattaattg gcatgggaag tgccctgttt
     4861 atgattgttt cgaaatatgg ttttgctgac gtgctgtctt tagatcacgt cggactcgac
     4921 cccagccgta ttgctgctca ggtggtgacg ggcgtcgggt ttatcggtgc aggtaacatt
     4981 ctcgttcgta accaaaatat tgtaggtctg acgacggcag cggatatctg ggtgaccgcc
     5041 gcataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
     5101 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
     5161 atttacagct gacattagtg aacggtaata cggtatcaat gctcgactgg tttaagcagc
     5221 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
     5281 ttgatatcca gcttcatgcc acaacgttga tagaagacct gttgcgatta ttaaaaggta
     5341 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
     5401 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
     5461 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
     5521 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
     5581 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
     5641 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
     5701 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
     5761 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
     5821 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
     5881 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
     5941 acataaccga ctcctggtgc ggacattaac tttataatca gtttgccaaa ggtaattaac
     6001 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
     6061 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
     6121 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
     6181 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
     6241 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagcctgg
     6301 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactggatc
     6361 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggctt
     6421 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
     6481 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
     6541 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
     6601 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
     6661 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
     6721 ttcatctgat tctgaggtga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
     6781 ccttctgttt agttcaattc cgttattttg gggcgtggta tgagtatcca tcgcccaata
     6841 atataaccct ttgttttaaa attacataaa atctattttt attcaatgat tataaagtgc
     6901 taataatcaa tattatggta ttatattctg ataatgattt gataagcgaa ttcttttgcc
     6961 agatggtatt tctatttcca ccccataatt tatatctttt tcattaggca gatctctctt
     7021 taaataaaag gggtatgaaa tcttttcggg ttgaagtgtc tcgtaatgaa atgctatgaa
     7081 ttcattttta gacattgttg cccatttttg aattttattg tatagagagg gtgttgtaaa
     7141 ctcttgaagt tgtttataac atattttttt gaactcttgc ttatatttta atatgtcatc
     7201 aaatgaaaga ttcactacct ttgcctctaa gaaatcaatt attctcgaag caatttccgc
     7261 tttcgcgcgt aaagagttgt cattaatagt taacaatgta gaaaacaaac cattgatttg
     7321 tttgtcaaga tgttgttcat atttatgtag tttggggatg ggattttcct tgtgtgaaaa
     7381 aatagtgtta aataaatttg cgtcagtgaa atttatatta agtagatctt caaaataaaa
     7441 ttttgataag gttaggacta gcttggacat atcttgccct gtcatgttga aatcaataaa
     7501 gtcgcatcca gcaagggctt ccatcctatt ttcattttta ctgtgctcaa tatacagctt
     7561 cgccattttt aagtacaagc tttgaatgtc tcttatatca gttggtaagt cagctcgttg
     7621 cccagcaaaa tttttattat cggaagaaac ggtttggaca ctgaatcttt cgggtattcc
     7681 ttcttctacg agaattttag ttatgattgg attgttgggt tctggattaa cgacaacaat
     7741 gatttgacat acagaatcct tatccttttc gtatctaatg ttatgaatct gattaagtat
     7801 tgattgtgtc tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact
     7861 tacaagttga accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca
     7921 tgcattaatt tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc
     7981 tccagagaat gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg
     8041 tgtgtctggc gttttaacat tatcggtatt tgttgtgcag ttataaatat cgccgtgata
     8101 tgtatttatt atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt
     8161 ttatgctaat gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa
     8221 taaatataat gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac
     8281 ccggtaatat ttgtgtataa aaaaagggca gtgatagtat cgtaaaggaa aacgttaaca
     8341 actatcaaaa aacgctcatt ttaaaaagtt attatgttct ccgatagtgt gtgaagtaat
     8401 atttttgtga tgttttagat gtgtaataat gaaccaggtt aaagtgtatt taaaaatgtg
     8461 ttgtattagc gactgaaatc tgccttttaa aaaggaggag ggtactgaga caaagagtca
     8521 ttgcttctcc gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact
     8581 tgttgaccga gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga
     8641 ttatcctgtc tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt
     8701 tactgctggc atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac
     8761 ctaatataac cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata
     8821 tcacttccgt caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac
     8881 ccattccttt tgtcatcaga acagatgcca gagggataac cgcctcagcc cggatataca
     8941 gcggaatacc gacaacggcg cttaatggga tggcgaaggg attatctgct cctgcatgag
     9001 cggcaatcca cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca
     9061 aaagatatgg cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg
     9121 gattaatact tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt
     9181 tagccgctgg actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt
     9241 ttgctggagc acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc
     9301 ccagagaatc caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca
     9361 acaaggtgac tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta
     9421 atggtgaaac aaataaaaaa gtgagagtcg gaccaaaccc ggctttcgct gacaacaatc
     9481 cacgtaacat gggtattgtc gagcaactac agaacggggt aacggctccc aacagagcag
     9541 ccaggagata gcctctccct tttctggccc ccatcatctg ctggatttta tggtctggca
     9601 ccttttgttt tatcaggctg acaccggcac taatcactat aaacagcaac gaaagctcga
     9661 cggcgagaaa cacaaacatc tc
//
LOCUS       DAJTRB010000134         9643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTRB010000134 DAJTRB010000000
VERSION     DAJTRB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.134"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO330_003700"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="OO330_003700"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5884408.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="OO330_003701"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="OO330_003701"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5884409.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="OO330_003702"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="OO330_003702"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5884410.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="OO330_003703"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="OO330_003703"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5884411.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="OO330_003704"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="OO330_003704"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5884412.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="OO330_003705"
     CDS             5060..6193
                     /locus_tag="OO330_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5884413.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="OO330_003706"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="OO330_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5884414.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO330_003707"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="OO330_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5884415.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO330_003708"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="OO330_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5884416.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO330_003709"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="OO330_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5884417.1"
                     /translation="MMKRLIVLVLLASTLLTRCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO330_003710"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="OO330_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5884418.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="OO330_003711"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="OO330_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5884419.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2450 a         2471 c         2460 g         2262 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtcccct gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcgcgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tct
//
LOCUS       DAJTRB010000135         9595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTRB010000135 DAJTRB010000000
VERSION     DAJTRB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.135"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(6..326)
                     /gene="psiF"
                     /locus_tag="OO330_003712"
     CDS             complement(6..326)
                     /gene="psiF"
                     /locus_tag="OO330_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5884420.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(445..1860)
                     /gene="phoA"
                     /locus_tag="OO330_003713"
     CDS             complement(445..1860)
                     /gene="phoA"
                     /locus_tag="OO330_003713"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5884421.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYATDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLEPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO330_003714"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO330_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5884422.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTALLRDYVKKLLKHPCQ"
     gene            complement(2403..2606)
                     /locus_tag="OO330_003715"
     CDS             complement(2403..2606)
                     /locus_tag="OO330_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884423.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="OO330_003716"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="OO330_003716"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5884424.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVRDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="OO330_003717"
     CDS             complement(3802..4014)
                     /locus_tag="OO330_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5884425.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="OO330_003718"
     CDS             4274..4582
                     /locus_tag="OO330_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5884426.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO330_003719"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO330_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5884427.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO330_003720"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO330_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5884428.1"
                     /translation="MFKSFFPKPGTFFLSAFIWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="OO330_003721"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="OO330_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5884429.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="OO330_003722"
     CDS             complement(8478..8981)
                     /locus_tag="OO330_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5884430.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9069..>9595)
                     /locus_tag="OO330_003723"
     CDS             complement(9069..>9595)
                     /locus_tag="OO330_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5884431.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2380 a         2448 c         2285 g         2482 t
ORIGIN      
        1 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
       61 agagcttgtt gcgttgcttg attattacat tcacgcatct tttgctgctg tggcgtcaaa
      121 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
      181 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
      241 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
      301 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg
      361 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
      421 gaaaattcac tgccgggcgc agttttattt cagccccaga gcggctttca tggtgtagaa
      481 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
      541 acgcaactga ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac
      601 catcactgcg ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg
      661 cgcaacaacc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc
      721 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
      781 ttgcccacaa gggttcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
      841 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgtgccag
      901 tgttggtaca ctgtcattac gttgcggatt aggcgtatag gtgactgcgg gcttatcgat
      961 attgccgtgg tacgttgctt tcggctctag ccagcgcact ggcatattgc cgtcagcaaa
     1021 caggccaagc aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
     1081 gttcaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
     1141 accagcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg
     1201 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
     1261 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag
     1321 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
     1381 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
     1441 gccgttatag gttttgacac cggttgacca ggcggttgct gatgcagccg agtcggtggc
     1501 gtagtccggt ttgccagttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacaa ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagcg ctgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtccgcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat ttatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attattaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagtagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaataaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat taccagtatt tcttcggagt
     9061 gaagaatatt accaagtata tttaacaccc acgttcgcgg accagtcttg atctacgtca
     9121 ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga
     9181 gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc
     9241 acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga
     9301 gcctgatctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg
     9361 ctgtcgtaag gctgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac
     9421 tggaacagac cagaaatgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag
     9481 tcgtaaccgg ttttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa
     9541 gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct caagc
//
LOCUS       DAJTRB010000136         9538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTRB010000136 DAJTRB010000000
VERSION     DAJTRB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.136"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..67
                     /locus_tag="OO330_003724"
     CDS             <1..67
                     /locus_tag="OO330_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5884432.1"
                     /translation="CARAGDKLPDLLDEYIRTTTE"
     gene            39..1967
                     /locus_tag="OO330_003725"
     CDS             39..1967
                     /locus_tag="OO330_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5884433.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            1951..2154
                     /locus_tag="OO330_003726"
     CDS             1951..2154
                     /locus_tag="OO330_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5884434.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2151..3744
                     /locus_tag="OO330_003727"
                     /pseudo
     CDS             2151..3744
                     /locus_tag="OO330_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3734..5239
                     /locus_tag="OO330_003728"
     CDS             3734..5239
                     /locus_tag="OO330_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5884435.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASDRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5276..5623
                     /locus_tag="OO330_003729"
     CDS             5276..5623
                     /locus_tag="OO330_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5884436.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5681..6709
                     /locus_tag="OO330_003730"
     CDS             5681..6709
                     /locus_tag="OO330_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5884437.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6761..7147
                     /locus_tag="OO330_003731"
     CDS             6761..7147
                     /locus_tag="OO330_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5884438.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7159..7536
                     /locus_tag="OO330_003732"
     CDS             7159..7536
                     /locus_tag="OO330_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5884439.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7523..8107
                     /locus_tag="OO330_003733"
     CDS             7523..8107
                     /locus_tag="OO330_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5884440.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSVWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8104..8505
                     /locus_tag="OO330_003734"
     CDS             8104..8505
                     /locus_tag="OO330_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5884441.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8516..9256
                     /locus_tag="OO330_003735"
     CDS             8516..9256
                     /locus_tag="OO330_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5884442.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9315..>9538
                     /locus_tag="OO330_003736"
     CDS             9315..>9538
                     /locus_tag="OO330_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5884443.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNA"
BASE COUNT         2218 a         2253 c         2952 g         2115 t
ORIGIN      
        1 gtgcgcgcgt gcgggggata aattaccgga tttactcgat gaatatatca gaacaacaac
       61 tgagtaatat gatggtggca gtcactatcg cactgcagcc actggtcagg gtattgccgg
      121 tgacggctgt agaatgggcc gatcaaaatt attacctgcc taaagaatcg tcatacggtg
      181 agggggaatg gaaaacgctg ccgtttcagg tcgccatcat gaactgcatg ggcaatgacg
      241 agattcgtac agttaacctg attaaatctg ctcgtgttgg ttatacaaaa atggtgcttg
      301 gtgtgatcgg gtattttatt gagcacaaat cccgcaacac tcttcttttt cagccgacgg
      361 actcagctgc tgaagatttt atgaagtctc acgtggaagc aacaattcgt gatgttcctt
      421 gcctgaaaaa actttctccc tggctgggcc ggaaacaccg ggataatacg ctcacactga
      481 agcgtttttc ctctggtgtg gggttctggt gtttgggtgg tgctgctgcc aaaaactacc
      541 gtgaaaaatc tgtcgatgtg gtctgctatg acgaactctc ctcgtttgaa ccggatgtgg
      601 aaaaagaagg ttcgccaaca ctgcttggcg ataaacgtat tgaaggttcg gtatggccaa
      661 aatccatacg cggttcaacg ccaaaaatta aaggctcctg tcagattgaa aaagctgcga
      721 atgaatctgc gcatttcatg cggttttatg tcccttgccc tcattgcggg gaggagcagt
      781 atctgaagtt tggcgatgat gcgacgtcgt ttggcctgaa atgggagaag ggtaaaccgg
      841 aaacggtgta ttacctgtgt gaacataatg gctgcgtgat ccgtcagtct gaacttgatc
      901 agaccggggg gcgctggatt tgtgacaata ccgggatgtg gacgcgtgac ggtctgacgt
      961 ttttcagtgc ttcgggtaat gaaattccgc cgccgcgctc catcactttc catatatgga
     1021 cggcgtacag tccgttcacc acctgggtac agatagtctg tgactggctg gatgcactga
     1081 aagatcccaa cggcgtgaaa acttttgtga acaccacgct gggcgagacc tgggaagagg
     1141 ccgtgggcga aaaactcgat caccaggtac tgatggataa ggttgtgcct tacacggcaa
     1201 cggtgcctgt ccgggtggtt tatctgacgg cgggcattga ctcgcagcga aaccgttttg
     1261 agatgtatgt ctgggggtgg gctccgggag aggaagcctt tctggtggat aaaatcatca
     1321 tcatgggacg tcctgatgag gaagagacgc tgttacgtgt ggatgcggcg atcaataaaa
     1381 aataccgcca tgcagacggt accgaaatga ccatttcccg tgtctgctgg gatatcgggg
     1441 ggatcgatgg cgaaatcgtg tatcagaggt caaaaaaaca cggtgttttc cgggtactgc
     1501 cggtaaaagg tgcctctgtc tatggcaagc cggtgatcac catgccgaaa acccgcaatc
     1561 agcggggtgt gtatctgtgt gaggtgggga cggataccgc aaaagaaatt ctctatgccc
     1621 gaatgaaggc cgatcccacc cctgtgaatg aggccacatc gtatgccatc cgctttcctg
     1681 ataatccgga gattttttcg cagacagagg cacaacaact ggtggcggag gaactggtgg
     1741 aaaagtggga aaaaggaaag atgcgtctgt tgtgggataa caaaaagcgg cgtaacgaag
     1801 cgctggactg cctggtgtat gcctatgcgg cattacgtgt gtccgtgcaa cgctggcaac
     1861 ttgatctggc tgtgctggcg gcatcccgca aggatgcaac tgcacaaccg acccttgaag
     1921 aactggcagc gaagctgtcc ggaggagtga atggttacag tcgctgaact gcaggcgctg
     1981 cgtcaggcgc gccttgattt attaaccggt aaacgggttg tgtcggtaca gaaagacggt
     2041 cgcaggatcg aatatacggc agcgtccctg aatgagctta accgtgcgat caacgatgcg
     2101 gagtcggttc tgggtacgac ccgacgccgc cgaccgctgg gagtgaggtt atgaaacgaa
     2161 cgcctgtcct gattgatgtg aacggcgttc cgcttcgtga gagtctcagc tacaacggag
     2221 gcggcgcagg atttggcggg caaatggcag agtggttacc accggcgcag agtgccgatg
     2281 cggccctgtt gcctgcattg cgtctgggga atgcccgggc agatgatctg gtgcgcaata
     2341 acggtatcgc ggctaatgcg gtggccctgc ataaggatca tatcgtcggg cacatgtttc
     2401 ttatcagcta ccgtctgaac tggcgttggc tggggatgcg ggagaccacg gcaaaaagtt
     2461 ttgtcgggga ggtggaagca gcctggtcgg aatacgcaga aggaatgttt ggcgagatcg
     2521 acgtggaagg gaaacgcacg tttacggaat ttatccgtga aggggtgggc gtgcatgcct
     2581 ttaacggcga aatctttgtg cagccggtct gggatacgga aaccacgcag ttattccgta
     2641 cgcgttttaa agccgtgagt ccgaaacggg tggacacgcc aggacacggt atggggaacc
     2701 actttctgcg ggccggggtt gaggtcgatc ggtatgggcg ggcgattgcg taccatatct
     2761 gtgaggatga ttttccgttc tctggtgccg gacgctggga gcggatcccg cgtgagcttc
     2821 ccaccgggcg tccggccatg ctgcatattt ttgagccggt ggaggacggg cagacccgtg
     2881 gggctaatca gttttacagc gtaatggagc ggatgaagat gctggactcc ctgcaggcca
     2941 cgcagcttca gtcggccatt gtgaaggcga tgtatgcagc cacaatcgaa agtgaacttg
     3001 ataccgaaaa ggcatttgaa tatatcgcgg gggcaccgca aggtcagcag gataatccgc
     3061 ttattaatat tctggagaag ttcacccgct ggtatgacac gaataacgtg acgctgggcg
     3121 gtgttaaaat tccgcacctt ttttcctggc gatgcgctga acctgcagac agcgcaggat
     3181 tcagacaatg gattttcggc gcttgaacag gcgctgctgc ggtatattgc agccggtctt
     3241 ggcgtttcct acgaacagtt gtcccgtgat tactcgaagg tcagttactc aagtgcccgc
     3301 gcctccgcca atgagtcgtg gcgctatttt atggggcggc gaaaatttat tgcggcccgg
     3361 ctggccacgc agatgttttc ctgctggctg gaagaggcgc ttcttcgggg gattattcgt
     3421 ccgccacggg cgcgttttga tttttaccag gcgcgttcag cctggtcacg ggcagagtgg
     3481 attggctccg gaagaatggc cattgacggg ctcaaggagg tccaggaatc ggtgatgcgc
     3541 attgaggccg gactgagcac gtatgagaaa gaactggcac tgatgggcga agattatcag
     3601 gacattttcc gccagcaggt cagggaatcc gcagagcgcg aaaaagccgg actctcacgt
     3661 ccggtgtgga tagcccaggc gtatcagcag cagatagcgg agagtcgcag gccggaagag
     3721 gagacaacat cacgtgagac gtaatctttc acatattatc gctgcggcat tcaatgagcc
     3781 gctgcttctg gagcccgcct atgcgcgggt tttcttttgc gcgctcgggc gtgagatggg
     3841 cgcagcaagc ctttcggtac cgcaacagca ggtacagctt gacgctcccg ggatgctggc
     3901 tgaaacggac gagtacatgg ccggaggcaa acgacctgcc cgtgtttacc gggtggtgaa
     3961 cggtattgct gtactgccgg tgaccggcac gctggtacac cggctgggtg gcatgcggcc
     4021 attttccgga atgactggct atgacggcat tgtcgcctgt cttcagcagg caatggcgga
     4081 cactcaggta cggggcgtac tgctggacat tgacagtcct ggcgggcagg ccaccggcgc
     4141 gtttgactgc gctgacatga tttaccgtct ccggcagctg aagcctgtct gggcactgtg
     4201 caatgacacg gcctgttcag ccgccatgtt actggcatcg gcctgctccc gacggctggt
     4261 tacccagaca tcccgtatcg gctccattgg ggtgatgatg ggccatgtca gctatgccgg
     4321 tcatctggca caggccggtg tggatatcac gctgatttac gccggatcgc acaaggtgga
     4381 tggcaatcag tttgaatccc tgccagcaaa ggttcgccag gacatgcagc agcgcattga
     4441 tgcggcgcgc cggatgtttg ctgaaaaagt ggcgatgtat accggtttat ctgtggatgc
     4501 ggttatggga acagaggccg ccgtttttga aggtcagtcc ggtattgagg ccgaactggc
     4561 ggatgaatta atcaatgcgt cggacgccat cagcgtgatg gctgcgacgc tgaacacaca
     4621 cgatacagga ggcactatgc cgcaattaac tgcaacggag gctgccgcgc aggagaacca
     4681 gcgagtgatg gggatcctga cgtgtcagga agcgaaagga cgtgaacaac tggccacgat
     4741 gctggcaggg caacagggta tgagcgttga gcaggcccgg gcgattctgg ccgtggcggc
     4801 accgcaacaa tctggtgtat ctgcgcagag tgaagccgat cgcattatgg catgtgaaga
     4861 ggcgaaagga cgtgaacaac tggcggcaac gctggcggcg atgccggaga tgacggtgga
     4921 aaaagcccgt ccgattctgg ctgcttcacc gcaggcgaat gccggaccct cacttcgtga
     4981 tcagattatg gccctggatg aggcaaaagg ggcagaggcg caggctgaaa aactggcggc
     5041 ctgtccgggg atgaccgtgg agaatgcccg ggctgtactg gcggcagcct cagacagggc
     5101 agaaccggtt tctgcatcca caaccgccat gtttgaacat ttcatggcga accattcacc
     5161 ggcagcggta cagggtggcg tgccacagac gtcagcagac ggtgatgcgg acgtgaaaat
     5221 gctcatggcc atgccatgaa gtcagtgctg accatcaata tgaggttttt acaatatggt
     5281 aacgaaaacc atcactgaac agcgtgcgga agtacgtatt tttgccggta atgatccggc
     5341 ccataccgcc acaggcagca gcgggatttc ctcggcaaca ccggctctga cgcccctgat
     5401 gctggatgag gataccggga aactggtggt ctgggacgga cagaaggcag gccatgtggt
     5461 tggcatactg gtactgccgc ttgaaggtac agagacggtg ctgacgtatt acaagtcggg
     5521 gacctttgcg acggaggcca tccactggcc tgagagagtg gatacacaca aaaaggcgaa
     5581 tgcctttgcc ggcagtgccc tgagtcacgc agcgctgccg taacatttta tcaggtcact
     5641 gcggtagcct gactgatttc tgaatgaaag gaactgattt atgggattgt ttacgacccg
     5701 ccagttactc ggttataccg aacaaaaagt taaatttcgt gcgctgtttc tggagctgtt
     5761 tttccgccgt acggtgaatt tccataccga agaggtaatg ctggacaaaa ttaccggaaa
     5821 aacgccggtg gcggcctatg tctccccggt tgttgaagga aaggtgttgc gtcaccgtgg
     5881 cggtgaaact cgtgtactgc gcccgggcta cgttaagccg aaacacgaat ttaattacca
     5941 gcaggcggtg gagcgtcttc ccggtgaaga tccggcccag ctgaacgacc cggcttaccg
     6001 tcgcctgcgt atcatcaccg ataacctcaa acaggaagag cacgccattg tccaggtgga
     6061 agagatgcag gcggtgaatg ccgtgctgta tggcaaatac accatggaag gagagcagtt
     6121 tgaaactgtt gaggtcgatt ttggccggtc ggtgggaaat aacattacgc aggcaggagg
     6181 gacggagtgg agcgctcagg accgcgatac ctttgatccg acgcatgatc tggatgcgta
     6241 ctgtgatttt gcttccggca ccatcaatat tgccattatg gatggtaagg tctggcgtct
     6301 gctgaatggc tttaagctgt tccgcgaaaa actggatacc cgtcgtggct caaattcaca
     6361 gctggaaacg gcagtgaaag acctgggggc ggtggtgtcc ttcaaagggt attatggtga
     6421 tctggcaatc gtggtggcaa aaacgtccta tgtggcagag gacggtaccg aaaaacgtta
     6481 tctgccggac ggcacactgg tactgggaaa tacggccgca gagggtattc gttgctacgg
     6541 tgccattcag gatgcgcagg cattgtctga aggcgtggtg gcgtcttccc gttatccgaa
     6601 acactggctg accgtgggtg atccggcgcg tgaattcacc atgacgcagt ccgcgccgct
     6661 gatggtgctg ccggatccgg atgagtttgt ggtggtgcag gtgaaataat ccgtgagcgg
     6721 gagctaaatg ctcccgtttt tcattcaatc aggagcgggt atggctacga aagaagaaaa
     6781 cctgaaacgt ctttgtgagc tggctgagag gctggggcgt gaaccggatg tctccggtag
     6841 tgcggcagat attgcgcagc gagtggcgga actggaggaa gagcttggag atgcggggga
     6901 ggtcgcagag ccggatacgt actcaccatc gaaagatgat ccgaccgcgc ataaaggcga
     6961 gtcagttcct gaacagccag gcattgttac cggagacgaa acggggcagg ttacggtggt
     7021 ggcactggcg acactgcata caaaatgtaa tggttcagtg atatttgttt caccgggaac
     7081 gtcgtttcgt gtttctgctg aggtggctgc cagcatggcg gcaggtggtc tggcaaaaag
     7141 gcagtaacag gagatgtcgt ggacggtttc gataatctgt ttgatgctgc gcttgccggt
     7201 gtcaatgaag tcattctccg tgatatgggg atcagcgccg tcattacgtc cggagaactt
     7261 gaggggacac acctgacagg ggtttttgat gatcccgaaa gtatttcttt tgttgccggg
     7321 gggatccggc ttgaggattc ctcaccgtgc ctgtttgtga aaacggcaga tatatcgcag
     7381 ttacgccgtc aggatacgtt aactatcggg gatgattctt tctttgtgga tcgtattact
     7441 ccggatgacg ggggatgctg ttatatccgg ttaaggcggg gcagtcctgc cccacagaat
     7501 aatgccagaa tgaggcgata tgatgaaggg acttgagaat gccatccgca atctgaacag
     7561 cctcgatacc cgcatggtgc cacagaccag cgtatgggcg gtaaaccgtg tggcggcaaa
     7621 gattgtttct gtcgccacac ggcaggttgc gcagaatacc gttgccgggg ataaccaggt
     7681 gaaaggtatt cccctgaaaa cggtcaggga gcgtgtcagg ctgcttaaag ccagcccctc
     7741 aggaaaaatg tatgcccgca tgcgtgttaa ccggggtaac ctgcccgcca ttaagctggg
     7801 taccgcacag gtcagactgg cccgctcccg gcatggcagc aactcacgtc atcgtggcag
     7861 cgtactgaaa gtggggaaat accttttccg ggatgcgttt attcagcaac tggcgaatgg
     7921 tcgctggcat gtgatgcggc gtattgacgg caaaaatcgt taccccattg atgtggtgaa
     7981 aatcccgatg tccggaccgc tgacacaggc atttgaagat gcccgcgacc gcatcattgc
     8041 tgcggaaatg ccgaaacagc tggggtatgc cctgaaacaa caactgaggt tacatctgag
     8101 taaatgaacc gacacacaca aatccgtcag agcgtgctgg cacgccttcg ggaacagtgt
     8161 ggagacagcg ccacgttttt tgacgggctt ccggcattta ttgatgcgca ggaactgcct
     8221 gccgtggcgg tgtggctgag tgatgctcag tacaccggaa aaatgacgga tgaagatgac
     8281 tggcaggctg ttctgcatat tgccgtcttc atccgggcac aggcaccgga ttcagagctg
     8341 gatatgtgga tggagagcac cattttcccg gccctgaatg atgtaccggc actttccgga
     8401 ctcatcgaca ccctgaaccc actcggtttt aactatcaac gtgataatga gatggctacc
     8461 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
     8521 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
     8581 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
     8641 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
     8701 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
     8761 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
     8821 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
     8881 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
     8941 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
     9001 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
     9061 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
     9121 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
     9181 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     9241 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     9301 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     9361 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     9421 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     9481 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctga
//
LOCUS       DAJTRB010000137         9486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTRB010000137 DAJTRB010000000
VERSION     DAJTRB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.137"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..183)
                     /locus_tag="OO330_003737"
     CDS             complement(<1..183)
                     /locus_tag="OO330_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5884444.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGE"
     gene            703..1398
                     /gene="minC"
                     /locus_tag="OO330_003738"
     CDS             703..1398
                     /gene="minC"
                     /locus_tag="OO330_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5884445.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1422..2234
                     /gene="minD"
                     /locus_tag="OO330_003739"
     CDS             1422..2234
                     /gene="minD"
                     /locus_tag="OO330_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5884446.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2238..2504
                     /gene="minE"
                     /locus_tag="OO330_003740"
     CDS             2238..2504
                     /gene="minE"
                     /locus_tag="OO330_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5884447.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2876..3220)
                     /locus_tag="OO330_003741"
                     /pseudo
     CDS             complement(2876..3220)
                     /locus_tag="OO330_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3255..3374)
                     /locus_tag="OO330_003742"
     CDS             complement(3255..3374)
                     /locus_tag="OO330_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5884448.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3335..3520)
                     /locus_tag="OO330_003743"
     CDS             complement(3335..3520)
                     /locus_tag="OO330_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884449.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3621..3794
                     /locus_tag="OO330_003744"
     CDS             3621..3794
                     /locus_tag="OO330_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884450.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3796..4140
                     /locus_tag="OO330_003745"
     CDS             3796..4140
                     /locus_tag="OO330_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5884451.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4144..4479
                     /locus_tag="OO330_003746"
     CDS             4144..4479
                     /locus_tag="OO330_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5884452.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4536..6857)
                     /locus_tag="OO330_003747"
     CDS             complement(4536..6857)
                     /locus_tag="OO330_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5884453.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6974..7183)
                     /locus_tag="OO330_003748"
     CDS             complement(6974..7183)
                     /locus_tag="OO330_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884454.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7934..9457)
                     /locus_tag="OO330_003749"
     CDS             complement(7934..9457)
                     /locus_tag="OO330_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5884455.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2202 c         2077 g         2548 t
ORIGIN      
        1 ttcgccgaga tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata
       61 cgggtcgcaa acaacgccgc acgtcggaga ttgaagtccg ccagcgtcat gtccagacgc
      121 cattgatgag aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt
      181 catagcacat cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt
      241 aatcaaaacc gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct
      301 gcggtttcta tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt
      361 aatgatgctg caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc
      421 ctcaatactg gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag
      481 gaagcgtaaa taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt
      541 tttaagctat gaatcagcgc catttatcac agaatagact tttactctga ataaatggga
      601 gggtgacttg cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat
      661 cgcgcgctgg cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cactccaatc
      721 gagcttaaag gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag
      781 gttatccatc aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc
      841 cccgttgtac tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag
      901 gcggtttcgg caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt
      961 aaagccgaaa ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca
     1021 cgtccagctc ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt
     1081 ttaatagata ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt
     1141 gttacaagcc acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat
     1201 ggcatgatgc gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt
     1261 tgtacgaacc tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa
     1321 atcccagcag aattttatgg caaagcggcg cgactgcagt tagtcgaaaa cgctttgacc
     1381 gttcaaccgt taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg
     1441 ttacttcggg taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt
     1501 tggcccagaa gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg
     1561 acctgattat gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg
     1621 atgcaacgct aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc
     1681 cggcatcgca aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg
     1741 atgatctgaa agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa
     1801 ccggtgcgtt aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag
     1861 tctcctcagt acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg
     1921 cagaaaatgg cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc
     1981 gcgtaagcag aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac
     2041 tcgtcggcgt gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccag
     2101 tcattctcga cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt
     2161 tgggagaaga acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct
     2221 tgttcggagg ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag
     2281 ccaacattgc aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag
     2341 aaccgcatta tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac
     2401 aaattgatcc tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc
     2461 ttgagctgaa cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg
     2521 catttatctt caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt
     2581 gtggtaaagc ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt
     2641 acaccttaag gctgttccca cgcacagaac aaaatgttta tgcttggttg agtaaataac
     2701 catgttatta gtgtgtatat actgggagat gataaccccg caatttcatt attcattgtt
     2761 ctcccatagg atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct
     2821 ctttcaacat cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga
     2881 acgaccagtt cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat
     2941 accccacccc gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt
     3001 atattccacg tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc
     3061 gattatcatc gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac
     3121 cgttccccag acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg
     3181 tatcgactga tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa
     3241 gctctgctgc aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc
     3301 gcaggtgtat tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg atatcccgca
     3361 taagaaagag gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc
     3421 ttttcagata atactgtcgt acgcttatct ttagacaatg aggccgtcgg aaaaataaaa
     3481 tgactaatgt aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata
     3541 ttgataaatc agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg
     3601 aataaaaata ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa
     3661 aaaacataat tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc
     3721 gttctttagt taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa
     3781 taccggacat ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc
     3841 tgctccacgc cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca
     3901 ggcgttggtt tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc
     3961 gctggtgttg gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca
     4021 aaagtgggtg ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg
     4081 gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa
     4141 ggaatgatga tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc
     4201 tgggcgcagg actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca
     4261 gaagccaacg aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg
     4321 ggtaatgcca gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc
     4381 aaagtggtag aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg
     4441 gcaattgatg acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg
     4501 atgaatacga cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc
     4561 atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt cccccagacg
     4621 ctggccgctg gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc
     4681 ccatcttgtt cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc
     4741 cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga
     4801 gtacgaataa aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca
     4861 atacgtgagc cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt
     4921 gcctgtggct ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg
     4981 gcaaaaccgt aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag
     5041 gctaattcat cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg
     5101 tatgcgcctt ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag
     5161 cctctgacac tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg
     5221 cgggcataac cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt
     5281 aacagatcgc cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg
     5341 ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc
     5401 tgttcagtta caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca
     5461 aaaagagtgt tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg
     5521 ggggtaggcg caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt
     5581 gcctggaagt tagtcagata ccagttttta ttggtcgctt cggtattacc tcgggttaat
     5641 gaatattcgt aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc
     5701 ccctcaacgc tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg
     5761 gtaactttaa ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct
     5821 gaattatcat catccaattc agttctgaga tgcaatacgc cattattacc aacgtaattt
     5881 tcagttattg ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc
     5941 gtactattat caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta
     6001 ttatctgaaa gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc
     6061 gttatttttg aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca
     6121 tctgcagccg atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg
     6181 tagaggattt catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc
     6241 tgaccattta tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta
     6301 tttttcagcg tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt
     6361 gtataaattt ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca
     6421 ttgtccccat gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca
     6481 agcgtgtcac cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg
     6541 tgggttgtga tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt
     6601 cctgtatcgg cagtactttc ttcatcaaga gaagatacac acgcacacgc gcttccggga
     6661 gatgcacacg caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct
     6721 ccgtcagaaa aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta
     6781 ttcaggtttc cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc
     6841 gtgtttttac cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt
     6901 aatgctgggc ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg
     6961 ccattgttaa tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac
     7021 ccgttgttat aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag
     7081 catgtttata ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag
     7141 caataagtcc caaactaaac ccggggagtt ttttcagttt catagattaa ctatccattc
     7201 attggattaa caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg
     7261 gtaaagacaa aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat
     7321 gataaataag tcggcattat catttttcac cttattttca tgacattgat cactttgggg
     7381 tgactatttt gtttaacaaa taactcttcg acatcaaaat tattaatagt tgccatggta
     7441 attatttcgg tgatatataa aaacaactgt gataacacca aggttgacaa ctatttgcgc
     7501 aataattcct ttccttcaat gatccaggcg ttcactgcca gcaacccatt cagccgcata
     7561 ctgacagtga gaaactgaaa gattattgct gatccctgtg gctaacgagt ctttctctta
     7621 aatcgattaa ccagattgat cgccagccaa acaagggcat taagcaaatt tatcgtcaca
     7681 agcacttctc ccagcccggt actgggtttt gcaataagga tgaccaggag ggtgatcagc
     7741 ataaatgcaa tggaaaagat gatatagaga gtgaaataat ccagattatt actattattc
     7801 atgctactaa tggcaatcct gtcaaaactt tcaaacaatt atagcaaaca aaataatgaa
     7861 caatagtaac aagaggcaac gagctaatta gctgtctata attacaccgc actatggcct
     7921 tactgatcaa ttttcactca accataacct tcaccttcgg tttagaaagg ataattttga
     7981 ccaggtcgat gtatgtaaca ggtttataga aataataacc ctgctgaaat gtgatattat
     8041 tttggttcag atagccaagt tgttctttcg tttcgacacc ttcagcgacg atactgatcg
     8101 aaagtttacg cgccagatcc aatacacaat ccagaattcg ggttgattct gggtctgcgt
     8161 ttacgcggcc aacgaaactg tgatcaattt tgatataatc aatatgcagg tcatgaagat
     8221 aagaaagccc tgagtagcca gtaccgaaat catccagcgc gatgacaaaa ccattttcat
     8281 gcagaatgtt caaccgctga accagacttt catcaacatt caatggctca cgctcggtga
     8341 cttccagaac aaggtttaaa tcgcggcggg taaaactgtc acggaaattt aaacactcgt
     8401 cgacaaacgt cggcgaaata atatgcgaag cgctaaaatt aattccaata tgaaaacctt
     8461 ccggcagttt actcgcgata gcgttcatct gtctggcaac ctgattcatc aggctttgtg
     8521 taagcggaac gattaatccg gatttttcag caagtggaat aaatgccgcg ggtgaaatat
     8581 atccaccgtg aggttgtttc cagcgggcta acacctcaac tccccgcaat gtcccttccc
     8641 gaccatttac cacaggttgg taaaaaggga cgatctcccc attatttatt gcccgtcgca
     8701 ggatctcttc aggcgttgcg cttttattga aatatctatc aagcaggaag gcagcggcgc
     8761 aggcgattaa cagaataaat attaaaatcc caaagccttt atcgataagt ctgtagaagc
     8821 taaagagtgg cggcgggtta aagataatag tgaaatgata ttttgtggag tgaaccttct
     8881 cggtataggg ataatgtcca ttaagcgtca tcacatcacc cgttggtcca ataatgtcat
     8941 ccgccacgcg taatacatac cttacccctt tcaagggtac attaagcgcc ccgcgaatat
     9001 gttgatcact tatcgtaacc aaaatgcgcg tatctgcaaa ttggctctga tagagcagga
     9061 taggtaatct attaacggtg tcgattgccg gagccaacag taaattactg tccgggacaa
     9121 caggaatacg agacagcagg acccgattcc caggcaggga tgtacaccag actattccct
     9181 gtttgagaat gatgattgtg cgcaggtgag gtttcagagc tgcttcagtg ccaagctgat
     9241 attgctcctc gaggtcgcat tccttcccgg caatgttaac cgctgtttga gtcgcctgtg
     9301 acgcttcttc aagtatatga ttgatattgt tggcggcata tcttgctccc gccaggtact
     9361 ctgcttttgc ggaataccag agctggatgt tcaaaatagc gacaccggta ataaacaagc
     9421 atatcgcaac gaggatgggt atgagtgtat tgcgcatttg cttgtcccgg ccaaagggtt
     9481 taatac
//
LOCUS       DAJTRB010000138         9438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTRB010000138 DAJTRB010000000
VERSION     DAJTRB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.138"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            429..1547
                     /locus_tag="OO330_003750"
     CDS             429..1547
                     /locus_tag="OO330_003750"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5884456.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="OO330_003751"
     CDS             1613..1861
                     /locus_tag="OO330_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5884457.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="OO330_003752"
     CDS             1926..2294
                     /locus_tag="OO330_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5884458.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="OO330_003753"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="OO330_003753"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5884459.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /gene="ybdG"
                     /locus_tag="OO330_003754"
     CDS             3149..4396
                     /gene="ybdG"
                     /locus_tag="OO330_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5884460.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO330_003755"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO330_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5884461.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO330_003756"
     CDS             complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO330_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5884462.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9096..>9438)
                     /gene="cusB"
                     /locus_tag="OO330_003757"
     CDS             complement(9096..>9438)
                     /gene="cusB"
                     /locus_tag="OO330_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR5884463.1"
                     /translation="QLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALIDTGNE
                     QRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSEANISGALE
                     RMRSESATHAH"
BASE COUNT         2315 a         2618 c         2284 g         2221 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgccccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcactt tattgccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgccccctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaagaaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 ccgatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 aggcaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccactag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 taaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgctgcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctca gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac agcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatcttcgg
     8281 gccaatctgg accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt
     8341 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
     8401 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
     8461 gctttttact tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac
     8521 ctgatactct ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt
     8581 cagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc
     8641 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
     8701 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
     8761 cgaacgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
     8821 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
     8881 cggataagtc acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat
     8941 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca
     9001 ggtgccccag atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga
     9061 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
     9121 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
     9181 ccggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat
     9241 gcctggaaaa cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc
     9301 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
     9361 gcggtgttga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
     9421 acttccagac gcagctgc
//
LOCUS       DAJTRB010000139         9270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTRB010000139 DAJTRB010000000
VERSION     DAJTRB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.139"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..92
                     /locus_tag="OO330_003758"
     CDS             <1..92
                     /locus_tag="OO330_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004205614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR5884464.1"
                     /translation="LLAGDSLCEFTVKERNIEFRAVLAYEPKK"
     gene            443..595
                     /gene="hokE"
                     /locus_tag="OO330_003759"
     CDS             443..595
                     /gene="hokE"
                     /locus_tag="OO330_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5884465.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(717..1337)
                     /gene="entD"
                     /locus_tag="OO330_003760"
     CDS             complement(717..1337)
                     /gene="entD"
                     /locus_tag="OO330_003760"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5884466.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1512..3752)
                     /gene="fepA"
                     /locus_tag="OO330_003761"
     CDS             complement(1512..3752)
                     /gene="fepA"
                     /locus_tag="OO330_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5884467.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKNENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3995..5197
                     /gene="fes"
                     /locus_tag="OO330_003762"
     CDS             3995..5197
                     /gene="fes"
                     /locus_tag="OO330_003762"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5884468.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5200..5418
                     /gene="ybdZ"
                     /locus_tag="OO330_003763"
     CDS             5200..5418
                     /gene="ybdZ"
                     /locus_tag="OO330_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5884469.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5415..9248
                     /gene="entF"
                     /locus_tag="OO330_003764"
     CDS             5415..9248
                     /gene="entF"
                     /locus_tag="OO330_003764"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5884470.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQVQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQVAFLG
                     LLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGNYADA
                     VRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDIISPG
                     AFVKIGPIIRATLNR"
BASE COUNT         2017 a         2442 c         2560 g         2251 t
ORIGIN      
        1 cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca
       61 gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata
      121 tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga
      181 tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac
      241 aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg
      301 cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat
      361 atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc
      421 ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct
      481 gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt
      541 aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt
      601 gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt
      661 acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa
      721 tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc
      781 tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga
      841 aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca
      901 agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt
      961 gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca
     1021 acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg
     1081 ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta
     1141 tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta
     1201 cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca
     1261 caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag
     1321 gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga
     1381 tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt
     1441 aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc
     1501 tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta
     1561 taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg
     1621 gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg
     1681 acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct
     1741 ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg
     1801 ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt
     1861 gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcattc
     1921 ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac
     1981 gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg
     2041 ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta
     2101 cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg
     2161 ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg
     2221 gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc
     2281 ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc
     2341 gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc
     2401 cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa
     2461 atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg
     2521 gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc
     2581 cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg
     2641 ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta
     2701 aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca
     2761 tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg
     2821 tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca
     2881 gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc
     2941 agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg
     3001 atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga
     3061 tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg
     3121 aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct
     3181 tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg
     3241 ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct
     3301 gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca
     3361 ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc
     3421 ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg
     3481 ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg
     3541 atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc
     3601 gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt
     3661 gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc
     3721 aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa
     3781 ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt
     3841 gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc
     3901 attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca
     3961 atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga
     4021 gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga
     4081 ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt
     4141 atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca
     4201 gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg
     4261 cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg
     4321 cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa
     4381 tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc
     4441 gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat
     4501 ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt
     4561 aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat
     4621 gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta
     4681 tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga
     4741 tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag
     4801 cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta
     4861 tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg
     4921 gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga
     4981 agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat
     5041 gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg
     5101 tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat
     5161 cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc
     5221 gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg
     5281 ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag
     5341 tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag
     5401 ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga
     5461 tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa
     5521 ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag
     5581 atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg
     5641 cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta
     5701 ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac
     5761 cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt
     5821 atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca
     5881 tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg
     5941 atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat
     6001 tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag
     6061 cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat
     6121 tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga
     6181 cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta
     6241 tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc
     6301 tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcagcac
     6361 aactgaaaaa aatgcgtcgt catcaacgtt acgatgccga acaaattgtc cgtgacagcg
     6421 ggcgagcggc aggtgatgaa ccgctgtttg gtccggtact caatattaag gtatttgatt
     6481 accaactgga tattcctggt gttcaggtgc aaacccatac cctggcaacc ggtccggtta
     6541 atgaccttga actggccctg ttcccggatg aacacggtga tttgagtatt gagatcctcg
     6601 ccaataaaca gcgttacgat gagccaacgt taatccagca tgctgaacgc ctgaaaatgc
     6661 tgattgccca gttcgccgcg gatccggcgc tgttgtgcgg cgatgtcgat attatgctgc
     6721 caggtgagta tgcgcagctg gcgcagatca acgccactca ggttgagatt ccagaaacca
     6781 cgctgagcgc gctggtggca gaacaagcgg caaaaacacc ggatgctccg gcgctggcag
     6841 atgcgcgtta cctgttcagc tatcgggaaa tgcacgagca ggtggtggca ctggcgaatc
     6901 tgctgcgtga gcgcggcgtt aaaccagggg acagcgtggc ggtggcacta ccgcgctcgg
     6961 tctttttgac cctggcactc catgcaatag ttgaagctgg cgcggcctgg ctaccgctgg
     7021 ataccggcta tccggacgat cgcctgaaaa tgatgctgga agatgcgcgt ccgtcgctgt
     7081 taattaccac cgacgatcaa ctgccgcgct ttagcgatgt tcccaattta accaaccttt
     7141 gctataacgc cccgcttacc ccccagggca gtgcgccgct gcaactttca caaccgcacc
     7201 acacggctta tatcatcttt acctctggct ccaccggcag gccgaaaggg gtaatggtcg
     7261 ggcagacggc tatcgtcaac cgcctgttgt ggatgcaaaa tcattatccg ctcacaggcg
     7321 aagatgtcgt tgcccaaaaa acgccgtgca gttttgatgt ctcggtgtgg gagtttttct
     7381 ggccgtttat tgccggggct aaactggtga tggctgaacc ggaagcgcac cgcgacccgc
     7441 tcgctatgca gcaattcttt gccgaatatg gcgtaacgac cacgcacttt gtgccgtcga
     7501 tgctggcagc atttgttgcc tcgctgactc cgcaaaccgc tcgccagagt tgcgtgacgt
     7561 tgaaacaggt tttctgtagt ggtgaggcct taccggctga tttatgccgc gaatggcaac
     7621 agttaaccgg cgcgccgttg cataacctgt atggcccgac ggaagcggcg gtagatgtca
     7681 gctggtatcc ggcttttggc gaggaactgg cacaggtgcg cggcagcagt gtgccgattg
     7741 gttatccggt atggaatacg ggtctgcgta ttcttgatgc gatgatgcat ccggtgccgc
     7801 cgggtgtggc gggtgatctc tatctcaccg gtattcaact ggcgcagggg tatcttggac
     7861 gacccgatct gaccgccagc cgctttattg ccgatccttt tgctcctggt gaacggatgt
     7921 atcgtaccgg agacgttgcc cgctggctgg ataacggcgc ggtggagtac ctcgggcgca
     7981 gtgacgatca gctaaaaatt cgcgggcagc gtatcgaact gggcgaaatc gatcgcgtga
     8041 tgcaggcgct gccggatgtc aaacaagccg ttacccacgc ctgtgtgatt aaccaggcgg
     8101 cagccactgg tggtgatgcg cgtcagttgg tgggctatct ggtatcgcaa tcgggcctgc
     8161 cgttggatac cagcgcattg caggcgcagc ttcgcgaaac attgccgccg catatggtgc
     8221 cagtcgttct gctgcaactg ccgcagttgc cacttagcgc caacggcaag ctggatcgca
     8281 aagccttacc gttgcctgaa ctgaaggcac aagcgccagg acgtgcgccg aaagcgggca
     8341 gtgaaacgat tatcgccgcg gcattcgcgt cgttgctggg ttgtgacgtg caggatgccg
     8401 atgctgattt ctttgcgctg ggcggtcatt cgctactggc aatgaaactg gcagcgcagt
     8461 taagtcgaca gtttgctcgt caggtgacgc cggggcaggt gatggtcgcg tcaaccgtcg
     8521 ccaaactggc aacgattatt gatggtgaag aggacagctc ccggcgcatg ggattcgaaa
     8581 ccattctgcc gttgcgtgaa ggtaatggcc cgacgctgtt ttgtttccat ccggcgtccg
     8641 gttttgcctg gcagtttagc gtgctctcgc gttatctcga tccacaatgg tcgattattg
     8701 gcattcagtc gccgcgccct catggaccca tgcagacagc gacaaacctg gatgaagtct
     8761 gcgaagcgca tctggcaacg ctacttgagc aacaaccgca cggcattgcg gcgcggctgc
     8821 gtgcccgtgg cgaacaggtg gcatttcttg gtttgctgga tacctggccg ccagagacgc
     8881 aaaactggca ggaaaaagag gctaatggtc tggacccgga agtgctggcg gagattaacc
     8941 gcgaacgcga ggccttcctg gcggcacagc agggaagtac ttcaacggag ttgtttacca
     9001 ccattgaagg caactacgct gatgctgtgc gcctgctgac gactgctcat agcgtaccgt
     9061 ttgatggtaa agcgacgctg tttgttgctg aacgcacgct tcaggaaggt atgagccccg
     9121 aacgcgcctg gtcgccgtgg attgcggagc tggatatcta tcgtcaggat tgtgcgcatg
     9181 tggatattat ctctccaggg gcgtttgtaa aaattgggcc gattattcgc gcaacgctaa
     9241 acaggtaaat taatattatc tataaaccca
//
LOCUS       DAJTRB010000140         9167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTRB010000140 DAJTRB010000000
VERSION     DAJTRB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.140"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..458)
                     /gene="pgaB"
                     /locus_tag="OO330_003765"
                     /pseudo
     CDS             complement(1..458)
                     /gene="pgaB"
                     /locus_tag="OO330_003765"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
     gene            complement(462..1955)
                     /gene="pgaB"
                     /locus_tag="OO330_003766"
     CDS             complement(462..1955)
                     /gene="pgaB"
                     /locus_tag="OO330_003766"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCR5884471.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYLSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            complement(1964..4387)
                     /gene="pgaA"
                     /locus_tag="OO330_003767"
                     /pseudo
     CDS             complement(1964..4387)
                     /gene="pgaA"
                     /locus_tag="OO330_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            4902..5462
                     /locus_tag="OO330_003768"
     CDS             4902..5462
                     /locus_tag="OO330_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCR5884472.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            5405..5794
                     /locus_tag="OO330_003769"
                     /pseudo
     CDS             5405..5794
                     /locus_tag="OO330_003769"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            5774..6331
                     /locus_tag="OO330_003770"
                     /pseudo
     CDS             5774..6331
                     /locus_tag="OO330_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            6429..8038
                     /locus_tag="OO330_003771"
                     /pseudo
     CDS             6429..8038
                     /locus_tag="OO330_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8139..8630)
                     /locus_tag="OO330_003772"
     CDS             complement(8139..8630)
                     /locus_tag="OO330_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HCR5884473.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(8635..8811)
                     /locus_tag="OO330_003773"
     CDS             complement(8635..8811)
                     /locus_tag="OO330_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5884474.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         2596 a         1789 c         1937 g         2845 t
ORIGIN      
        1 ttatgcagaa aattgtcggg ataataacca tagtttttca ctccattcag ttgtaatagg
       61 ctcatccagt gagcgagttg ttgcgaagga atagcctgat gctgaccatt tttctgccag
      121 ttttgtgcct gtaattctaa aatagatttg tctttagcct gagggatgtt tttaatttga
      181 ttggtcaatt gtattaacca ttggtcagcc gatttttctg cgacaccttc catataaggc
      241 atagccataa tagcggtcca gtcatagctt tttaggaaat cagcataatt ctgtgcaaac
      301 caggcttcac tttcaggttg tattaccgga agtgcaaaaa tatttcgtgc agttttaata
      361 tgtggaccgc gaatggcttt tacgcgcgca ctaagttcta aagtgaagtc agttaacgca
      421 cgacttttaa agcgggccca ctgtttaaat tgctccgggt tttatcgaat ttcgctcaga
      481 ctcccgctaa agcctgcttg ctgataagcc gtgatagccg gtgcactggc atcttcataa
      541 tctgaaagca aagcatcatc gtggaacaat atgccatcaa aagcagcatg tccggcaaga
      601 tcttcatata acatgccaac ttgtgctctg actctgtcat cgaaaggaga gagacggtga
      661 tattgttcag gatgaatttg tgcttttttc tcccctgttg gtaagtattt tactcgcgtt
      721 aatgtgggat ctaaatccca gcttaatacc ggcatccacg catagatgtt tacacctgag
      781 cgggtacgta attgccaggc aacccgacta aaaatatctg ctttcattgg tagcaaatga
      841 tttggaaacc agacctcttt gaccagccca tcaccatcgg gatcagcaaa tgcctgcaaa
      901 tacacggttg atatttgcat atctttcacc cgctgaatta gcacatcaat attgcgatcc
      961 atttgctggc ggttttcgtc ataaacgtaa tcaagatcga tatgcattat ccgttgtggt
     1021 gatttttcct gtacggtaat aattttctgg gcaaactctt ttaatgaggg attattggcg
     1081 attaatactc gcggaatgga atccaattgt gacgcatttg ccaaacctga ttcaagggtg
     1141 aaaaacatgt cataaccgag tttttttaat tcctctatcg ctatgccatt cgcttcgcca
     1201 taaggccaaa caaaaacgtg tggatttacc tcagcctttg tacgcaggta ttccgtcatt
     1261 tttacagcat ccagacgaat tctttcccgg tattctgctg cggtttcata ccgtgcgtgg
     1321 tcagtaaaat atgcacgatt tacatataca ggcattaagc tgccggtgga attagcctga
     1381 ataccgtagt gagaattcca tgtatgagaa gcgacctcaa cgagccggga acgcgcaact
     1441 tctcgcactt gttgccacgt ggcaaaatat tctcgatcga ccatctcatc gccaaatttt
     1501 acttgttcat ccactggcgt atcgacccaa ctgccgacgg gggcccatac agcaggccac
     1561 tggaaggcct gaagaattgg gaagacgcgg gtataaaaac tcaggtagcc gtcatcaaaa
     1621 gtcagcacta ctgctttttc cggtagcggt tttcctcctc gatgtgcttc acgaatttga
     1681 gcaatactga ccggttgata accgttctcg cgcagccagg caaattgttc acgcagtgct
     1741 gatgtccgca ctgacataaa acgctggtcg gcagcttcgt cttcaacgtt atgccatgaa
     1801 atcgccacaa aaccattatg cggccacggt tgctcggcga gtaaagattc gcgatcctgt
     1861 ggcggtataa atgatgttct tgactggctg atgcacgcgg tgagcataat tatactcacc
     1921 agcatcagga gatatttatt tccattacgt aacatattta tccttaaaat ctgaatgtca
     1981 tatcgaattc aacgtataag ttgtgttctc tgtcaccgtc ataaggtcgt ttttcccagc
     2041 gtagcgttgc gccagcatca atcacgtcat tccagctaat gcgttgcccg tagccgagtt
     2101 gggtgacgac atccgtgcca taatgttttt gccaggaggc accaacacct gcgctgaaga
     2161 tttgctccca gctattttca tagcttcgcc ataacaaatg acttgcctca aatgccggaa
     2221 caatatcgaa cgtttttata gggttgtagt atggggtatc gtgttctgta ttttgttcgt
     2281 aatacagact gggtaggaaa tcgacaatca aatatggtga agaccagatg cgttcctgac
     2341 cctcaattga gacttcatga cgctggttac tgtcggaaaa atcagtgaaa gcccaggaga
     2401 caccgtactt acgccgctca ttttgatacc agcgaacata agcctgagca ctgttgcctg
     2461 taacaccatt tttcattgcc cgtaatggaa cgcggtgaga gaggcgttcc agttgcgaac
     2521 aaatacgcca gttatcatta aaatcatacc agccagacag gcgcgcgccg ggtttatgct
     2581 catgattgaa aaagtgttca gcgtactccg cctcgagcca gatattacgt gaccgccact
     2641 caacacccgc cagccagtcg cgaacaatcc cttttccttc gctaaattgt ccatcggcat
     2701 aaccgaatcc agcaaaaccg cgccagttat ccttcagcgg tggtgaataa acgatggtgg
     2761 ttaagtcgac atcatgttta ccactatccg ggccttcggc atcaattcct gttgaaccag
     2821 ctatacgaag ctctgcaaga ttatgtacat caaccgcacg ttttaaacgt acaacgccgg
     2881 gatcttgcgg ttcacgttcg acaacatcgt gcgttaagac agctgcctgc tgccattctt
     2941 gtaacgttaa tgctgtctag gcttgttcaa cctccagatt aatattacgt ggctcgatca
     3001 cttctgcttt ttttaattca ttttctgctg cacgaggcca accgcgggct tgtaacacac
     3061 tcgcgtaatc aatgcgcagt ccctgatttc ctggtgcgtt ataagcaagc tctctggctg
     3121 tcatttcagc ctgaggaaga tcattactat attttgctac ggttgagaga aacgaatgcc
     3181 cctgtaacta ggtatcattc gggatgctcg taggcgtgcc cattaaccga aggaaaggcg
     3241 gcgaagtatt aatggtatgt tgggtgacag ttagcgcgcc cggataattt tcactctcta
     3301 ggtggctgta aaagagatcc gcaagttctt catcggataa atccggggca atggtctcct
     3361 tgtgataaaa gagctcggtc attattgact gtgctttttt cggctgatga tctttgagat
     3421 aagccgatgc aacccaatat tgcccctagg gcggaataat ttgccccgtc ttttttaatc
     3481 gctgatagtg agaaatgacg tctttataac gatcgcgagt taataacgcg ccaagatgat
     3541 caacctgaat acgctggtac tgggcagtgc ggtctgggtt atcgtgccac agaatttcta
     3601 atgcagcgta ttgggcgagg gcgcgattgg caatggcata acgttcactt tcactgcgcg
     3661 taggcataaa cgacagtctg accagttcgg catgaatatc agcgcgaata tctggcgtta
     3721 aattggcatc gtcaatcgcg gcagcaagtt gattattacg taatgcctgc acgtattctg
     3781 tgggatattg ttgcgtagat gcattttcag gtaatgactc tgtcatcgcc cgtaattcat
     3841 cctgatgacg ccccgccagt ttatagatat aggcttctgt gagtaaattg gctttgtccg
     3901 gtgctccaga gttaagctgc ttaagtttaa ccagcgcagt atcatagtga cccgcatctg
     3961 ccagggttaa aatttgtccc cgttgataat ccttattttg cggctccaga gagagcgcct
     4021 tttgccacag tgtaagcgag ttttgccatt gttgcaggtt acgataagcg acggcgacag
     4081 ctgcataacc acgcgctggt aattgctgat gacggtagcg gttgtaaacg gtaataacct
     4141 gtttatcttg cccggcccat aaggcaatct gtaaccagtc agcaatttgg ttattgctga
     4201 gtgctgattt tagtgcaaac catgacaaag ctggctgagt attacccttg cgagcttcaa
     4261 taatcaatgc atcataggcg ttattaacag cactcttcgc cgcgggactc gctgctaaaa
     4321 atgcggcagt aagaagtttc aaagcccatt tggttttcgg gcaccttttt ctgctacttg
     4381 aatacatcct gtattactcc atgtattgcc aaaatctctc tctgtatcta attacaggta
     4441 actgaaaaga aagatatttt tgcacctcat aatccgttat taaacgcgga agagagacgt
     4501 gaattgttga tgatgagaag aagaaatgat gagcagagtg tccatataaa atccttttct
     4561 cgcccgaaaa tccattccaa tgatgaggat cttcaggaat acggcataaa tcccaatgcc
     4621 tttttcaaaa taaattagga ttaaaataat taaatcagta aattccgatg catgatttcg
     4681 gatttttcga aagtcctgat gaaaggctgc gctacggcac tcacggattt aattgttatt
     4741 cctaatgtat ctatgaatta atgttttata aggattttct ataaccattt ataagtattt
     4801 tcaagcctgg cttgttgcaa acaatgtata aagcacttag gcaataataa ttacattcag
     4861 caactatcat catcggtatt gtttgtgggc ggaatttcat attgacaaca gtacagttct
     4921 tatatctatt aataatagaa agagatctac aacctacaga ttggtgtagc tttatggaaa
     4981 aagactattt gagaattagt agtactgtat tagtgagctt attgtttggg cttgctttgg
     5041 ttttggtgaa tagttggttt aatcagccag gcgttgagga agttgttcca cgatcaacct
     5101 atctgatggt aatgattgct ttatttttta tcgatactgt tgcatttatt tttatgcagt
     5161 tgtatttcat ttatgaccgt aggcaatttt caaactgtat acttagtttg gcttttctga
     5221 gttgtttgat ttactttgtt aaaaccgtca ttatcattca gcaaattatt gaggggcgtt
     5281 tgacaagcag tgttgtccaa aatgacattg caatctatta tttgtttcgt cagatgagtt
     5341 tgtgcatatt aatatttctg gcattggtga ataaagttag tgaaaacaca aaacagcgca
     5401 atttattttc caaaaaaatg actttgtgca taagtttgtt tttgtttttg gggggccgat
     5461 agttgctcac atactttcta gtcattatga gagctacaat ttacacattg ccgagttaac
     5521 caatgaaaat ggtcaggtgg tttggaaagc ctcatatgtt accataatga ttttcatgtg
     5581 gttaaccttg ctttcggtta atctatactt taatggttta cgctatgata tctggaatgg
     5641 agtgacggtg attgcttttt gtgccgtact gtacaatatc tctctgttat ttatgagcag
     5701 gtatagcgtg tccacttggt atattagtcg cactattgaa gtagtcagta agttaaccgt
     5761 tatggtaata tttatgtgcc atatattcag tgcgctacga gtaacaaaga acattgcaca
     5821 tcgcgatccc ttaaccaata tatttaacag aaattatttt tttaatgaac tgacagttca
     5881 atcagcatca gccaaaaaaa cgccttattg tgtcatgatt atggatatcg accacttcaa
     5941 agaagtcaac gacacctgag ggcatccggt tggcgatcag gtgataaaaa cagtggtgaa
     6001 tatcattggg aaaagcatac gaccagatga ccttttagcg cgcgtcggcg gcgaagagtt
     6061 tggcgtcttg ctgacggaca tcgatactga acgtgcgaaa gctttagcgg aaaggattcg
     6121 ggaaaatgtt gagcgtttaa ctggcgataa tcctgaatac gctataccac aaaaagtgac
     6181 gattagtatt ggcgctgttg tgactcagga aaatgcgtta aacccgaatg agatctatcg
     6241 actggctgat aacgcactat acgaggcgaa agagaccggg cgtaataagg tggttgtgag
     6301 ggatgtggtg agtttttgtg agtcaccata aagcggcatt ttagcgggtg attttggtaa
     6361 gcgctaaata gctattcgca gacttgcgta acaattttca gaaatatcat agtactatat
     6421 gatcttaaat gcttatcatt gatcaatctg ctatcacccg cttttactgg caattgtact
     6481 ataatttctt aggttgcatc accctgatgc gtggggaaac atgtatggta ttcaacaaaa
     6541 aaatgtttgt actcattata ataccaggca ttctgggggt gttactatcg tttgctatgt
     6601 caatatttca aatgaataga gatacgacaa ttaccgccgg tattctgtta aaacagcttg
     6661 ataatgttac gcaaattgtc cagcatacga caaagcttac ctctattctg gtgatgaagc
     6721 cctgcaaaga cattcttgaa cagttgattg ctaacggagc attgactccc tatgttcgaa
     6781 caacgggtct tatagaaaat aatttccaaa tatgttcttc agttagcggt ttcaaaaaaa
     6841 tgaatgttaa cgatgtttac ggtacgagct ttcacaataa aaataaagaa tcacgtatag
     6901 tatcaattag cggtacaagt tttgtgccag gtaaaacagc aatagtgttc cttatgccaa
     6961 tagggaatga tatgacagct tttagtattg tagagtcacg atatatctat gatctaatgg
     7021 atgttcttga tgatgagaat gatgattcat tttctcttcg ttttactgaa gggcctgcaa
     7081 ttattagcgg tgtaaataat aatgacaggt tatatatgtt aaaaagagat tttaattctg
     7141 cgatcagtca ggctcgtctt acagttacta cgccgatgat atcgttatat ccctatatca
     7201 ttagcaatgt gctttatatt ttgccgttat ctattattct ctcttttatt ctctatttct
     7261 tatggcagca ctggatgtca cggaaaatgt cgctggcaga agaaattaaa aaaggaatgt
     7321 catccggaga attttctgtg cattatcaac cagtgtgcga cacgacaacc aaagcgtgtc
     7381 tgggggttga agcgttaatg cgctggcaac gcgaagatgg taaaaatata tctccggtag
     7441 tttttatccg cgctgcagaa gaggaaaatt taataatacc gttaactaaa catctctttg
     7501 aactgattat tcaggatgta caaagctgga aagtgaaaaa gccgtttcac cttgggataa
     7561 atattgccgc ggaacatctt gcacatcctg actttgttgg tgatgtttta catattaaaa
     7621 atgcaatatc tgataaattt aatattgtcc ttgagattac cgaacgcaat ctggttgaag
     7681 acactgatct tgcattacag aaaataaatg aattaaggat ccatggctgt gaatttgcag
     7741 tcgatgattt tggcacggga tattgttccc ttggccttct gcaaaagttg tctgttgatt
     7801 atcttaaaat agataaaagc tttatagaca cacttactac agcggaagac gaaacgccag
     7861 ttttagatat tattattaag ctgagtaatc ggctcaattt gataacgatt gcagaaggtg
     7921 tcagtactcc tcaccagcag aatatcttat tcataataac gtgacattag ttcagggata
     7981 cttatatgaa aaaccaatga aagcgacaga gttttatcag tggtatgtaa acagataaat
     8041 aattaaaaat attggaatgg ttttgtattg tcttaaaata taaacaaagc aatcagaatg
     8101 ttattaactg atgacaaacg ccaggatcaa caatgctgtt agtgagtaat acatatgaat
     8161 cgtggtgtcc tgcctgtaga gaatatgtag ttataatcat tttgacgcgt aacataataa
     8221 ttaattgaag tgtctgcttt tatgaagaaa tcagaagtga tattttcaag gatttcatcg
     8281 ctgatgttat catctttttg tattttttgg attttaactg tggtgagata aacggtatca
     8341 cggaatttct tgtatgtaaa actcacatat cggcggatga agcccatttt ttttccatct
     8401 accgtgtatg cgcccgtgag atacatgttt cctgtgttat tttcagaaat catatttaac
     8461 gaaaaatatt gatccatttt tgtatgatcg gtgaaattaa taacatgggc attacatgat
     8521 atgttttgct ctgctggttc attggaatga tgaaacgcaa tatggctcag aattgctgca
     8581 ataaaagcac ataaaattat tcccgaaaac agaaaatatc tttgagtcat atgattactc
     8641 cgctttataa tagagagagt tgcaattatt tggtgattca gagtttaaca tttgactaca
     8701 ggtgaaagca taaaacccgc tcatgctatc atttgttgca atgtaaatgt aaggatgtat
     8761 tttgcagttg acttccattt tctgcaattt ttcaataatt ggtgatagca tgtctacgga
     8821 attaaagtaa ttattatcat agaaataact gcatccttcc tgagtagggt gttgagcata
     8881 atttgagaaa gcatctattt tttttatagt gtgagatttg ctttgaaaag ccatatcgaa
     8941 aaaataagaa gacatataaa ctatccccca tgccgcaaaa aaaaactata agtaatgtat
     9001 tctttgtggt aaatatacct tttgcaacgt aatttttatt tatattttca gtgtgggtgc
     9061 cattctcttc actctcaaca ctttcattct cattgtgatt attgtcgaga acagttatgt
     9121 tgatttcttc attgattttg aagcctttcc gtggcaccgt tctgata
//
LOCUS       DAJTRB010000141         9090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTRB010000141 DAJTRB010000000
VERSION     DAJTRB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.141"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..936
                     /locus_tag="OO330_003774"
     CDS             <1..936
                     /locus_tag="OO330_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5884475.1"
                     /translation="SLPGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1188..2105
                     /gene="oxyR"
                     /locus_tag="OO330_003775"
     CDS             1188..2105
                     /gene="oxyR"
                     /locus_tag="OO330_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5884476.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2088..3488)
                     /gene="sthA"
                     /locus_tag="OO330_003776"
     CDS             complement(2088..3488)
                     /gene="sthA"
                     /locus_tag="OO330_003776"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5884477.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISAVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3809..4459
                     /gene="fabR"
                     /locus_tag="OO330_003777"
     CDS             3809..4459
                     /gene="fabR"
                     /locus_tag="OO330_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5884478.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4459..4818
                     /locus_tag="OO330_003778"
     CDS             4459..4818
                     /locus_tag="OO330_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5884479.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4858..5958)
                     /gene="trmA"
                     /locus_tag="OO330_003779"
     CDS             complement(4858..5958)
                     /gene="trmA"
                     /locus_tag="OO330_003779"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5884480.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6072..6262
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6327..8171
                     /gene="btuB"
                     /locus_tag="OO330_003780"
     CDS             6327..8171
                     /gene="btuB"
                     /locus_tag="OO330_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5884481.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8116..8973
                     /gene="murI"
                     /locus_tag="OO330_003781"
     CDS             8116..8973
                     /gene="murI"
                     /locus_tag="OO330_003781"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5884482.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2068 a         2208 c         2452 g         2362 t
ORIGIN      
        1 tcattacccg gtctgggtat tggcgaagcg ccatttatgc ctgcgggagt aaagtcgatc
       61 accgactggt atgcacaaaa agaacgcggc acggcgctgg ggatttttaa ctcatctacc
      121 gttatcggtc aggctatcgc acctcctgct cttgtattga tgcagctggc atggggctgg
      181 cggacgatgt tcgttatcat cggcgtggca gggattctgg ttgggatctg ttggtacgcg
      241 tggtatcgca accgggcgca gtttgtcctg actgacgaag aacggacgta tctctccgcg
      301 tcggttaaac cgcgtccaca gctgcaattt agcgagtggc tggcgctgtt taagcatcgg
      361 acaacctggg ggatgatttt aggtttctct ggcgtcaatt ataccggttg gctttacatt
      421 gcgtggttac ccggttattt gcaagccgaa caaggtttca gtctggcgaa aaccggttgg
      481 gtagcggcga ttcctttcct cgcggcggcg gtcgggatgt gggttaacgg tattgtggtc
      541 gatcggctgg cgaaaaaagg ctacgacctg gcgaagacgc gtaaaacggc tattgtctgc
      601 ggtttgatga tgtcggcatt aggcacgttg ctggtcgtgc aatcttcctc gccagctcag
      661 gcggtagcgt ttatctcaat ggcgctgttc tgcgtgcatt tcgcgggaac gtctgcatgg
      721 ggactggtgc aggtgatggt gtcagaaaca aaagtggctt ccatcgccgg gattcaaaac
      781 tttggcagtt ttgtttttgc ttcctttgct ccgatcgtaa ccggttgggt agtggatacc
      841 acacactcgt ttaatctggc gctggttatc gcggcctgtg tgacgttcac cggggcgctg
      901 tgttacttct ttattgtcaa agatcgcatt gagtaatcgt tactatcagg ctctcttgct
      961 gtgggcctgt agaataaaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt
     1021 tagttattcg agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg
     1081 ccgctccgtt tctgtgagca attatcagtc agaatgcttg atagggataa tcgttcattg
     1141 ctattctacc tatcgccatg aactatcgtg gcgatggagg atggataatg aatattcgtg
     1201 atcttgagta cctggtggca ttggctgaac accgccattt tcggcgtgcg gcagattcct
     1261 gccacgttag ccagccgacg cttagcggcc aaattcgtaa gctggaagat gagctgggcg
     1321 tgatgttgct ggagcggacc agccgtaaag tgttgttcac ccaggcggga atgctgctgg
     1381 tggatcaggc gcgtaccgtg ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc
     1441 agggtgagac gatgtccgga ccgctgcata ttggtttgat tcctacggtt ggaccatacc
     1501 tgctaccgca tattatcccg atgctgcacc agacatttcc aaagctggaa atgtatctgc
     1561 atgaagcaca gacccaccag ttactggcgc aactggacag cggcaaactc gattgcgtga
     1621 tcctcgcgct ggtgaaagag agcgaagcat tcattgaagt gccgttgttt gatgagccaa
     1681 tgttgctggc tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta ctgatggccg
     1741 atctggcagg ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc gatcaggcaa
     1801 tgggtttctg ctttgaagcc ggggcggatg aagatacaca cttccgcgcg accagcctgg
     1861 aaactctgcg caacatggtg gcggcaggta gcgggatcac tttattgcca gcgctggctg
     1921 tgccgccgga gcgcaaacgc gatggggttg tttatctgcc atgcattaag ccggaaccac
     1981 gccgcactat tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc
     2041 tggcagaggc catccgcgca agaatggatg gccatttcga taaagtgtta aaacaggcgg
     2101 tttaaaccgt ttaacgcagc tacccgatag gcttccgcca tcgtcgggta gttaaaggtg
     2161 gtgttgacga agtactcaat agtgttgccg ccacctttct gttccataat cgcctgaccg
     2221 atatgaataa tttcggcagc gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt
     2281 tcccgatgga acaaaatttt cagcgtgccc acgttcatgc cgacgatttg tgcacgtgcc
     2341 agatgtttga actgggcgcg gcccacttca tacggcactt tcatcgcggt cagctgctgt
     2401 tcggttttgc ccacagcgct gatttccggg atggtgtaaa tgccggtagg gatatcttca
     2461 atcagatgtg cggtggcttc gccttttacc agcgcctgcg cggcaatgcg cccctggtca
     2521 taggccgctg atgccaggct cggataacca atcacgtcgc ccaccgcgta aacgtgcggc
     2581 tgtgcggtct gatacatgct gttgaccttc agctgtccgc gactgtcagt ttccagccca
     2641 atgttctgta acgccagcga atcggtatta ccggtacgac cgttggcata gagcaggcag
     2701 tcagctttca gttttttacc cgacttcaga tgcatgatca caccgtcgtc acagctttcg
     2761 atcttctcgt actcttcgtt gtgacgaatc actacgccac tgttccagaa gtggtaggag
     2821 agagaatctg acatctcttg atcgagaaat gccagcaggc gatcgcgggt gttgatcaga
     2881 tccactttta catccatacc gcggaagatc gacgcatatt cacagccgat cactccagca
     2941 ccatagataa gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc gctgtcgtaa
     3001 atgcgtggat gggtgaaatc aacatctgtt ggatgatatg gacgagagcc gcaggcaata
     3061 acaaattttt cagcggttag tgtttcaacg ctgccgtccg ggcaatccag cgccaacgta
     3121 tgctcgtcaa caaagcgagc gttcccctgc aatatttcac agtgattacg ttcgtaaaat
     3181 ccctgacgca tgcgcgtttg ttgattaatc acgttatcgg catggttaag gatatcggca
     3241 aaagaagagc ggagcagtcg ggaatggtcg ctgtatagtg ggttttgatt gaattctata
     3301 atgcggctga cggcgtgacg gagagctttc gacgggatgg tgccccagtg ggtgcaaccg
     3361 ccgccaacat tttgataacg ctcgataact gcgacgcgcg caccttgctt aaccaggccc
     3421 attgcagcgc cttcgccgcc ggggccggaa cctattacta tggcatcgta atcgtaggaa
     3481 tgtggcatgg tagggcttac ctgttcttat acataaaagc aacagaatgg taacattcta
     3541 tcgcgggtaa gccaattgat ccccgtcatt tatctggcta tatcctgagc ggcctttgct
     3601 ttgtctgttt cttacttttg ccctgacgtt ttattggatt tttatcgacg atactctccg
     3661 tttaagcggc aggtttccgc tgtacgtaaa agaaccggcc agtaaatatg ttttattgcg
     3721 ttaccgttca ttcacaatac tggagcaatc cagtatgttc attctctggt atagtgccag
     3781 cagtactttt ggcaaggatt cagacatcgt gatgggcgta agagcgcaac aaaaagaaaa
     3841 aacccgccgt tcgctggtgg aagccgcatt tagccaatta agtgctgaac gcagcttcgc
     3901 cagcctgagt ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct ctttttatcg
     3961 gcatttccgc gacgtagacg aactgggtct gaccatggtt gatgagagcg gtttaatgct
     4021 acgccaactc atgcgccagg cgcgtcagcg tatcgccaaa ggcgggagtg tgatccgtac
     4081 ctcggtctcc acatttatgg agttcatcgg taataatcct aacgccttcc ggttattatt
     4141 gcgggaacgc tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg aaattcagca
     4201 cttcattgcg gaacttgcgg actatctgga actcgaaaac catatgccgc gtgcgtttac
     4261 tgaagcgcaa gccgaagcaa tggtgacaat tgtcttcagt gcgggtgccg aggcgttgga
     4321 cgtcggcgtc gaacaacgtc ggcaattaga agagcgactg gtactgcaac tgcgaatgat
     4381 ttcgaaaggg gcttattact ggtatcgccg tgaacaagag aaaaccgcaa ttattccggg
     4441 aaatgtgaag gacgagtaat gaaacaagca aatcaagata gaggtacgct gctgctggcg
     4501 ttggttgctg gtttatcgat taatggtact ttcgcagcgc tgtttagctc cattgtgcca
     4561 ttttctgtat tcccgattat ttccctggtg ctgacggttt actgcctgca tcaacgttat
     4621 cttaatcgca ccatgtcggt aggcttgccg ggtctggcag ctgcctgttt tattctcggc
     4681 gtactgctgt acagcacggt agttcgtgcg gaatatccgg atatcggctc taacttcttc
     4741 ccggcagtac tctccgtcat tatggtgttc tggattggcg cgaagatgcg taaccgtaag
     4801 caggaagttg ctgagtaatc ggtatcatgc cggatgctgg cgcatccggc atgggtttta
     4861 cttcgcggtc agtaatacgc cgcactccat atggtgcgtg taggggaact gatcaaacag
     4921 agccagacgt tcgaccttgt gcgtctggct taatgtttcc agatttttgc ataacgtttc
     4981 cggattgcag gagatataca gaatacgtgg atacgcctgc accattttct cggtttcact
     5041 gtccagaccg ctgcgcggtg ggtcgacaaa aatggtttcg aactgatagc tctttaagtc
     5101 gatcccttgc aggcggttaa actcgcgcac accattcatc gcctgagtaa attcttctgc
     5161 cgccatacga ataatttgta cgttatcaat atggttagcg gcaatgttgt attgcgcggc
     5221 agcgactgac ggtttggcga tttcggtggc taatacccga tcaaaattgc gcgccagcgc
     5281 taatgaaaag ttaccgttgc cgcagtacag ctccagtaaa tcgccttttg agcctttggt
     5341 tacgtccagc gcccattcca gcatctgaat attcatcgcc gcgttcggct gggtaaagct
     5401 gttttctacc tgacgataga tcatctcttt ccctgcgacc ggcagacgct cgtcgatgta
     5461 atcttgatcc agcgcgattt tggttttcgt tgcccgacca atcagatgca cattcagatt
     5521 ctgcgcgcgc agtgcatcgc gcagtgcttc cgcttcctga cgccactcat catccagctt
     5581 tttatggtac agcagggaaa ccaccgcctg attactcagc gtggtgaggt aatcaatctg
     5641 gaacaacttg tggcgcagaa cgggattatt acgcacaccc gcaatcatcg ccgtcatcaa
     5701 ctggttgatg agttcactgg cggcggggaa gctatccacg cggatgcggc ttttggtttg
     5761 ttgatcgaaa atgatgtgat acaggtcatc gccatcgtgc cagatgcgga actccgcgcg
     5821 catccggtaa tgactgaccg gcgagcgaaa cacttccgga accaggtcag aaaacggtgc
     5881 catcatactt tgcaaacgta ccactttttc ggctaactgc gcttcatact gttctgttgg
     5941 aaggtgttcg ggggtcatga tgtatcctga aaaattaaag tacgcggcga ttgtagggat
     6001 tgctcatcag atgtccagat cttgatgaat tcctatttgt gagctacgtc tggacagtaa
     6061 cttgttacaa cctgtagcat ccacttgccg gtcctgtgag ttaataggga atccagtgcg
     6121 aatctagagc tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat gcggacactg
     6181 ccatccggtg ggaagtcatc atctcttagt atcttagata cccctccaag cccgaagacc
     6241 tgccggccaa cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa cctgcggcat
     6301 ccttcttcta ttgtggatgc tttacaatga ttaaaaaagc ttcgctgctg acggcgtgtt
     6361 ccgtcacggc attttccgct tgggcacagg ataccagccc ggatactctc gtcgttactg
     6421 ctatccgttt tgaacagccg cgcagcactg tgcttgcacc aaccaccgtt gtgacccgtc
     6481 aggatatcga ccgctggcag tcgacctcgg tcaatgatgt gctgcgccgt cttccgggcg
     6541 tcgatatcac ccaaaacggc ggttcaggtc agctctcatc tatttttatt cgcggtacaa
     6601 atgccagtca tgtgctggtg ttaattgatg gcgtccgcct gaatctggcg ggggtgagtg
     6661 gttctgccga ccttagccag ttccctattg cgcttgtcca gcgtattgaa tatatccgtg
     6721 gaccgcgctc cgctgtttat ggttccgatg caataggcgg ggtggtgaat atcatcacga
     6781 cgcgcgatga acccggaacg gaaatttcag cagggtgggg aagcaatagt tatcagaact
     6841 atgatgtctc tacgcagcaa caactggggg ataaaacacg ggtaacgctg ttgggcgatt
     6901 atgcccatac tcatggttat gatgttgttg cctatggtaa taccggaacg caagcgcaga
     6961 cagataacga tggtttttta agtaaaacgc tttatggggc gctggagcat aactttactg
     7021 atgcctggag cggctttgtt cgcggctatg gctatgataa ccgtaccaat tatgacgcgt
     7081 attattctcc cggttcaccg ttgctcgata cccgtaaact ctatagccaa agttgggacg
     7141 ccgggctgcg ctataacggc gaactgatta aatcacaact cattaccagc tatagccata
     7201 gcaaagatta caactacgat ccccattatg gtcgttatga ttcgtcggcg acgctcgatg
     7261 agatgaagca atacaccgtc cagtgggcaa acaatgtcat cgttggtcac ggtagtattg
     7321 gtgcgggtgt cgactggcag aaacagacta cgacgccggg tacaggttat gttgaggatg
     7381 gatatgatca acgtaatacc ggcatctatc tgaccgggct gcaacaagtc ggcgatttta
     7441 cctttgaagg cgcagcacgc agcgacgata actcacagtt tggtcgtcat ggaacctggc
     7501 aaaccagcgc cggttgggaa ttcatcgaag gttatcgctt cattgcttcc tacgggacat
     7561 cttataaggc accaaatctg gggcaactgt atggcttcta cggaaatccg aatctggacc
     7621 cggagaaaag caaacagtgg gaaggcgcgt ttgaaggctt aaccgctggg gtgaactggc
     7681 gtatttccgg atatcgtaac gatgtcagtg acttgatcga ttatgatgat cacaccctga
     7741 aatattacaa cgaagggaaa gcgcggatta agggcgtcga ggcgacagcc aattttgata
     7801 ccggaccact gacgcatact gtgagttatg attatgtcga tgcgcgcaat gcgattaccg
     7861 acacgccgtt gttacgccgt gctaaacagc aggtgaaata ccagctcgac tggcagttgt
     7921 atgacttcga ctggggtatt acttatcagt atttaggcac tcgctatgat aaggattact
     7981 catcttatcc ttatcaaacc gttaaaatgg gcggtgtgag cttgtgggat cttgcggttg
     8041 cgtatccggt cacctctcac ctgacagttc gtggtaaaat agccaacctg ttcgacaaag
     8101 attatgagac agtctatggc taccaaactg caggacggga atacaccttg tctggcagct
     8161 acaccttctg aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg
     8221 gtctatgacg agatccggca tctcttaccg gatctccatt acatttatgc tttcgataac
     8281 gtcgctttcc cgtatggcga aaaaagcgaa gcgtttattg ttgagcgagt ggtggcaatt
     8341 gtcaccgcgg tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg caacaccgcc
     8401 agtaccgttt cacttcctgc attacgcgaa aagttcgact tcccggttgt tggtgtcgtg
     8461 ccggcgatta aacctgctgc acgtctgacg gcaaatggca ttgtcggatt actggcaacc
     8521 cgcggaacag ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc taatgaatgc
     8581 cagatagaaa tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa gctgcatggc
     8641 gaagatgttt ctctggatgc actaaaacgt atcctacgcc cgtggttaag aatgaaagag
     8701 ccgccagata ccgttgtatt gggttgcacc catttccctc tactacaaga agaactgtta
     8761 caagtgctgc cagagggaac ccggctggtg gattctggcg cagcgattgc tcgccgaacg
     8821 gcctggttgt tagaacatga agccccggat gcaaaatctg ccgatgcgaa tattgccttt
     8881 tgtatggcaa tgacgccaga agctgaacaa ttattgcccg ttttacagcg ttacggcttc
     8941 gaaacgctcg aaaaactggc agttttaggc tgatttggtt gaatgttgcg cggtcggaaa
     9001 attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac
     9061 acggaacaac ggcaaacacg ccgccgggtc
//
LOCUS       DAJTRB010000142         9012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTRB010000142 DAJTRB010000000
VERSION     DAJTRB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.142"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO330_003782"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO330_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5884483.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO330_003783"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO330_003783"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5884484.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO330_003784"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO330_003784"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5884485.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO330_003785"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO330_003785"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5884486.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="OO330_003786"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="OO330_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5884487.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="OO330_003787"
     CDS             5545..6018
                     /locus_tag="OO330_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5884488.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="OO330_003788"
     CDS             6047..6589
                     /locus_tag="OO330_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5884489.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="OO330_003789"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="OO330_003789"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5884490.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDVLTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="OO330_003790"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="OO330_003790"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5884491.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="OO330_003791"
     CDS             7986..8243
                     /locus_tag="OO330_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5884492.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="OO330_003792"
     CDS             complement(8219..8773)
                     /locus_tag="OO330_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5884493.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2254 a         2188 c         2249 g         2321 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgtcctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag ag
//
LOCUS       DAJTRB010000143         8997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTRB010000143 DAJTRB010000000
VERSION     DAJTRB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.143"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OO330_003793"
     CDS             complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OO330_003793"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5884494.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OO330_003794"
     CDS             complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OO330_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5884495.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="OO330_003795"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="OO330_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5884496.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO330_003796"
                     /pseudo
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO330_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO330_003797"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO330_003797"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5884497.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="OO330_003798"
     CDS             complement(7826..8137)
                     /locus_tag="OO330_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5884498.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO330_003799"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO330_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5884499.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8649..>8997)
                     /gene="csgA"
                     /locus_tag="OO330_003800"
     CDS             complement(8649..>8997)
                     /gene="csgA"
                     /locus_tag="OO330_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5884500.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2250 a         2328 c         2135 g         2284 t
ORIGIN      
        1 gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgtggcgt gaacgcctta
       61 tccgacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc
      121 gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag
      181 tggattgagc tttatccctt cgtaataact cacccatgaa gaatatcttt caggggaatt
      241 ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg
      301 atcgcgattc agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc
      361 gatttccagc accttactgg cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt
      421 aaatgacggg ttaaacactg cgtgcataaa gccatcgtca agggagcgtt ggcgattcat
      481 ctcaaggaac cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag
      541 gaacagtttc cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac
      601 aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc
      661 cagccacgcc atcccaacag cccacactaa ccccaacagc agctgtgagc cgtggcgttt
      721 gaacgcttcg ccccatgaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc
      781 ccagccaagg aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag
      841 cagcacggaa aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta
      901 ttctttcgtt cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag
      961 caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg
     1021 gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgca ctacctgcaa
     1081 tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc
     1141 cgtcaggaac accgcacggt gaaccgggtg cataccctta accaggaaca gacggaagtt
     1201 catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg
     1261 caattcttca taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg
     1321 catcaacgcc gcttctacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc
     1381 cggcagcgga gccagtgcgc agtgctcgat aaatggtttc acgcggataa tcgcgttatg
     1441 tccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc
     1501 atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt
     1561 cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca
     1621 taaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct
     1681 gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg
     1741 gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt
     1801 ttgctctgcg acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg
     1861 tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg
     1921 gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg
     1981 ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag
     2041 gaagcccatc aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaaaag
     2101 gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc
     2161 ctgaccaacc atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt
     2221 catataccag gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg
     2281 gatggtacca acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc
     2341 cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt
     2401 ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg
     2461 atcgcgccct tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg
     2521 ttccagacgc gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg
     2581 ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc
     2641 gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact
     2701 ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag
     2761 tgattttctt ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg
     2821 ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg
     2881 tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg
     2941 cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac
     3001 gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg
     3061 tgatggtgta tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt
     3121 aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc
     3181 ctttgcccca ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat
     3241 cgagatcttc aaagcgggag aaatcacgac ctcgttgcaa cagaccaaag cctcgcgggt
     3301 tttccatgga gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc
     3361 attcgccatt accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt
     3421 tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc
     3481 ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac
     3541 gccctggcat aaccacgaat ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat
     3601 aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg
     3661 gaaattcttc acccgatggc aaagcggtat caattgctag gccgcgggca gaaaggccat
     3721 aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat
     3781 cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt
     3841 ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga
     3901 ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac
     3961 cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat
     4021 gattaaactg gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt
     4081 tgcttttagg cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat
     4141 caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc
     4201 aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa ctgaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctaacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggatactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt
     4921 accgcaattt attatgctgc aatatgtcaa agggaaagcg gaaagttggc ctgggctatc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgtgt atggatattt aagcgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccacccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt taccgttctt
     5281 tatcctcggc gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt accctcgctg cagcattggc gtttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttat tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taacctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcgtac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg caccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact gctttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc
     6541 gtcatggcat cgttatgcta ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa catcctctac cacggggcct attgccatga catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgcaccaaa ataggcatca ccaatatttc gtcctcccac tagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atccggcgat tgagacggga aaagtcggtg
     6841 atatatccaa gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cgtggatgac tgtcaagcaa gcgcaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgccccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta actataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgtagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttccccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc
     7921 tcattaacgc tttgctggag taggttttgc ccggaataga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 tccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca
     8521 tatccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa
     8641 atgatgtatt aatactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg
     8701 acggaggagt tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta
     8761 acagtcattt cagaattttt gccgttccac tgatcaagag tggcgctgtt accgaagcca
     8821 cgttgggtca gatcgattga gctgtcatct gagccctgac caacatctgc accattacca
     8881 ccgccatgct gggtaatagt caagtcagag ttacgggcat cagtttgcag agcaagtgca
     8941 gagttaccgc caccgtactg gtaaatgttc agctctgaat ttgggccgct attatta
//
LOCUS       DAJTRB010000144         8942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTRB010000144 DAJTRB010000000
VERSION     DAJTRB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.144"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..272)
                     /locus_tag="OO330_003801"
     CDS             complement(<1..272)
                     /locus_tag="OO330_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5884501.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="OO330_003802"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="OO330_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="OO330_003803"
     CDS             complement(1132..1338)
                     /locus_tag="OO330_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5884502.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="OO330_003804"
     CDS             complement(1445..2008)
                     /locus_tag="OO330_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5884503.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="OO330_003805"
     CDS             complement(3383..5218)
                     /locus_tag="OO330_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884504.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="OO330_003806"
     CDS             5319..5606
                     /locus_tag="OO330_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5884505.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="OO330_003807"
     CDS             5578..7107
                     /locus_tag="OO330_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5884506.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="OO330_003808"
     CDS             complement(7277..8494)
                     /locus_tag="OO330_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5884507.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
BASE COUNT         2768 a         2027 c         1674 g         2473 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aa
//
LOCUS       DAJTRB010000145         8939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTRB010000145 DAJTRB010000000
VERSION     DAJTRB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.145"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..2886
                     /locus_tag="OO330_003809"
                     /pseudo
     CDS             1..2886
                     /locus_tag="OO330_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OO330_003810"
     CDS             complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OO330_003810"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5884508.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3978..5330
                     /gene="yegD"
                     /locus_tag="OO330_003811"
                     /pseudo
     CDS             3978..5330
                     /gene="yegD"
                     /locus_tag="OO330_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OO330_003812"
                     /pseudo
     CDS             complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OO330_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7279..8040)
                     /locus_tag="OO330_003813"
     CDS             complement(7279..8040)
                     /locus_tag="OO330_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR5884509.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVIFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8037..8696)
                     /locus_tag="OO330_003814"
     CDS             complement(8037..8696)
                     /locus_tag="OO330_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5884510.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2180 a         2368 c         2328 g         2063 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccaaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc ccctttccgt tgccggtttg agtagcgcca cgctggtgaa tgatctgctt
     2461 gagcagctgg aaaatagccc tctaccacca cggttattac atctgattat tccggctgaa
     2521 gcgattttag atcacgcaga aagcgtgcaa aaactgcggc tggcgggatg tcggatagtg
     2581 ctcagccagg tgggccgcga tctgcaaatc ttcaactcgc tgaaagcgaa tatggcagat
     2641 tacctgctac ttgatggtga gttatgcgcc aacgtgcagg gtaatttgat ggatgagatg
     2701 ctgattacga ttattcaggg gcacgctcag cgactcggga tgaaaaccat cgccgggcca
     2761 gtcgttttac ccttagtgat ggatacgctt tctggcatcg gcgtcgatct gatttatggt
     2821 gatgtgattg ccgatgccca accgctggat ttgctggtga atagcagtta tttcgcgatt
     2881 aactgagaga gcgtttgccc attgcctgat gcgatgctaa cgcgtcttat caggcctaca
     2941 aatcgctcat tccgcaggca ggataaggcg ctcgcaccgc ttccggcgac caacgtcatg
     3001 cttcgtctgg ttgccagcct tccgtatacc agatatgcaa cagcgcataa gaacgccagg
     3061 gcttccagcg ctcggcataa cggcggattt gtgccggtgt catccccaga aatcgctgtt
     3121 taatcagata atcatccggc agaaaaacat ctttcgcctg ccagccgcgc aaggcgaaat
     3181 aattcgccgt ccagcgcccg atacccggaa aagtttgcag cgttttcatc gcctgctcca
     3241 catcgcccgg tattgtcatt ggtaaggtgc cctccagcgc cgcatttgcc agatgaatca
     3301 gcgcctctgc ccgtttcaac ggcataccta acgcttttaa tgcctgcggg tcggctgctg
     3361 ccagccgctg aggcgtgggg aagcagatat actccggaaa atcatccagc cgttcgccat
     3421 aaagctgtgc cactctggcg gtcaattttg ccgccatcgc cacgctcacc agttggccta
     3481 aaatcgcccg cacgccctgc tcaaaagcat caacactgcc gggtaaacgc aatccgggcc
     3541 gcgccgcgcc taacttgccc aacgcaccgt taacaatctg cgggttacat tgcagatcaa
     3601 gcaggcggct cattttcgcc agacactcag cggcaacggg ttctaaacct gcacttaaat
     3661 ttatgtgcag agtatggcgg gctatatccg gaatagcagt caccacgccg cgatattcgc
     3721 ccactgccag actacgggca taataactgt ccgcgaccgt ttccacacca ctcaccgcac
     3781 gggcggcgag aaatcccaac atccacgacc agtcatacgg cggctgccag ttcaggatat
     3841 acatcgcatc tccttattca tccgctttca gcataaacgt tattcagacg ttacgctttg
     3901 ctttcatatt ccggttgtcg cgacggcaac atttcgctaa agtcacgccc cttcttcacc
     3961 ggcatgggga ttatttcgtg tttattggtt ttgattacgg tacagcaaac tgttcagtgg
     4021 cggtcatgcg tgacggtaaa ccgcatttgc taaaaatgga aaacgacagc acgctgctgc
     4081 cttcaatgct ttgcgcgcca acgcgtgaag cggtaagcga atggctgtac cgccatcatg
     4141 acgttccggc agacgacgat gaaacgcagg cgttgttacg acgggcgatt cgttataacc
     4201 gcgaagaaga tatcgatgtt acagcgaaaa gcgtgcagtt cggtctttcc tcactggcac
     4261 agtacattga tgatccagaa gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg
     4321 ccagcggctt aaaaccgcag caggtagcgc tgtttgagga tctggtctgc gcaatgatgt
     4381 tgcacattcg ccagcaggcg caggcgcagc tgccagaagc gattactcag gcggtgattg
     4441 gtcgcccgat caacttccag gggctgggcg gcgatgaagc aaacgcccag gcgcagggga
     4501 ttctggaacg cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag tacgagccgg
     4561 tagcggctgg gctggattac gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg
     4621 atatcggcgg tggtacgact gactgttcat tgctgctgat ggggccgcag tggcgttcgc
     4681 gtctcgatcg tgaagccagt ctgctgggtc acagtggtta tcgtattggc ggtaacgatc
     4741 tggatatcgc cctagcgttt aaaaacctga tgccactgct gggtatgggt ggcgaaaccg
     4801 aaaaaggcat cgccctgccg atcctgccgt ggtagaatgc ggttgccatc aacgacgttc
     4861 ctgcacagag tgatttctac agtagtgcca acggtcgtct gcttaacgat ctggtacgcg
     4921 atgcccgcga accggaaaaa gtggccctgt tacagaaagt ctggcgtcag cgtttaagct
     4981 atcgcctggt gcgtagcgca gaagagtgca aaattgctct ttcaagcgta gcggaaaccc
     5041 gcgcctcact gccgtttatc agcaatgaac tggctacgct gattagccag cgagggctgg
     5101 aaagcgccct cagccagcca ctggcgcgga ttctggaaca ggtgcaactg gccctggata
     5161 acgcccagga aaaaccggac gttatctacc tgaccggcgg tagcgcccgt tctccgctga
     5221 ttaaaaaagc gctggcagaa cagttgccgg gcattccgat tgcaggcggc gatgactttg
     5281 gctccgtcac cgccgggctg gcacgctggg cggaagtggt atttcgttaa aagaaaatgg
     5341 ctcagcagcg gcttaaatca gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg
     5401 gttccatcaa cggcatggtc cgtgctggag catgaaggcg gaatcgctgc aactctgtcg
     5461 cgccgacagt caacgtcgat tccagccgat gtcgtttggc ggccattttc gccattaccg
     5521 cctgtctgtc tgctggcgtt accgcttcgt tcggcctgaa cacaaaacgg cgttcacagc
     5581 tcgctttcca gtcgatgacc gcctgggtca gatgatcacc aaacccttta acttgcttaa
     5641 cgccacgacg ggtaacacct gctgctgttt caataccaaa agaccataac gccgctttac
     5701 gcgcagggcc aacgccggga atagaggcaa catcaataaa aaatccctcc agaaacttct
     5761 acttctgccg ttcccttgca gtgtcatgaa gtgctgccag agcgagtttc tcttcttccg
     5821 gtaacccgag aatttcgtcc ttcatttttt cgagcatcgt ccgtttggcg ataaaacctt
     5881 ccaggccgcc tacctgctgg atctggcgga ctaaatggtc atagtccatt ttcgcacgat
     5941 taaatgcctc acggcgttgc tgaacttctg ctttgtacgc tttgcttgtc agactgccaa
     6001 taatccagat agccgccagc acaggaacca aaataatata acgcggttct gcctgaaggc
     6061 cgcaaagcag cagcgataat gctgacagtg cgatctcaag cagaatgatg tattcgcgcc
     6121 gtaacaggcc taaaggaagc ggcctgccag tcggttggaa atgatcgggt aatggcagtt
     6181 gcaatgctgg cggtgctact gacgccatca ccatcgccca gacttttgcc agcacaaaat
     6241 ccccgccggt ggtaatgacc tcttcgccga gatcaataaa atagataacg ccttgattat
     6301 ccagcgtaca ccacgggcag tcggtcaaat gagcgggata aacatgcatt gccgaaacgg
     6361 gacatttctt taactgttgg cgcagagaat caagtgccgc tacccacgct ttagccgtcg
     6421 gacgcccggt tgccacgcca ctttccgtga atgcctgctg aaacatggct tcaacatcac
     6481 tcggtaacat cgatagcgga atcgatcgtg gcggcggttt taaaccacgt cgctgattat
     6541 ctgacgcgta ggcataacgg aaatgggtaa tatccgtctc cagcgcatta cccgcatcag
     6601 agataagcgg cacaccagaa taaggatgcc ggccaccaaa caagacgtga aaaatcagca
     6661 acgcaaggcc aaaattaccg tgattttctg tgcgttcaaa gccgacaaat gacgacaagg
     6721 tttgtagctc tggcggcgta aaatgcgaca cgccgacttc gcataaatgc agtgtgccat
     6781 tggcgttaat ctgaaaggag tcgctatcaa tcaacaccac tttgctgtcg cgacctacca
     6841 taaaactgtt ctggtttacg tcacccacga catgcccgtg ctcgtgaacc gtagcaaaag
     6901 atgaagcaat attgcgcgca acatagagta gaaaatccca cgcacaatga gggtaacgtt
     6961 gacgacgatg tgccgggcta tagatcatat gaatcggttc tttaccagaa acttttggca
     7021 tcataaaacc gataactttt ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga
     7081 cataattcaa caattgcgcg tcagctgtcg cagccataaa ggcaagtttg tcctgtttta
     7141 aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc gacaaactca ttgatatcat
     7201 aaaccgcgcc ttcaccgcct ttgcccagtt cacggcctgg cgtaacgcac tcaccagtag
     7261 cagtatataa agtgggtttc atggtaacca cagcgccagt gccaacgttt tatcatcatc
     7321 cgtacgctcg tttaccgccg ggctggacaa aaactgcttt aacaattcag gtaataaatc
     7381 gagttgttcc tgcgttgctg acgccagtcc attgaaaaac ggggtaaaga aaggcacatg
     7441 cggagaatta tccaacatat ttaacgccag tcgctggata ccatccgtaa atgctgcaac
     7501 tttatgcaca cgctcagtgc tggtgaaagt ttccagtctg gaaacggcat cttcatcggt
     7561 aatgaagtgc gtcatattgg catattcacc caccatcggc gtaagcggca attgcagacc
     7621 gtgaccaaaa tccaccacca cgccgccatc gccaatctgc ataatcaagg tgccattagc
     7681 cgatgaaatc aatcccagaa acgtgcaggc aaaatcgcga actgctaatt ctttggcttc
     7741 cgcctcggca aaaagtcgct gacgaacggt cagcacaata tccgtggcga ggatatcatt
     7801 aagccccaat tccccaccct gcactttttg cgacatataa gccatcgctt cattgacggc
     7861 gagcatcgcg ccttcgcccc cctgcgaaac gctacctgcg ccatcagcaa ggaatatcac
     7921 cagtaatggc tgctggtcat ttagccaggc aacctgcatc tggcaggcat cctggcaagg
     7981 caagtccgcg ctgatgtgcg acgttccaac agcggatgca taaaccagac gccagctcac
     8041 actgacgtcc agccttttgg cgcttccagc acaacttccg ttcccggcgt ggagcgggaa
     8101 accgaacgca gcgaactgga taaccagcta aacagttcac ggaattgtaa tccttgcaga
     8161 ggcaaaggct gacgaacgct gatttgtgcc agagtcttca tatccgcccc ctgaacaccg
     8221 atggaaaaga aagcaaacct tttatcttct tccccccgaa acactttgtt ggctgcagcc
     8281 tgccactcat cgattggtgc accatcggta atcaggaaaa tccacgggcg ataatacgaa
     8341 ataccatttg cacgatactc acgtttccgc tcctctacca tatccagggc tttggtaatg
     8401 gccgcaccca ttggtgtatc gccctgggca aacaggatgg gcggaaaaaa attagcggcg
     8461 ctggtaaagg gttgttccac atgcaccggg ccgaacgtca caatgccgag ttccactctt
     8521 tttaaagcca gcgaatcggc aagcagctca tcgcgaaagg taaccaatcc ggtgttaagt
     8581 tcattaatag gtctgccact catcgagcca gaaacatcca gcagcagaat gcaggggcaa
     8641 cgtggttccg ggttactggc aaaatcgctg gtggcaaacg tgatttgttc gctcataaga
     8701 aatcctttct tagagaatta ttgaggtccg gtatcatacc gactgcaata atgaaataag
     8761 agcaaacgaa gaaactttca gaattagtcc cggcggcgca gaaaattttg ctattcgcct
     8821 cgcaagaacg gagagccgtc gattgacact aagggcggag tgacataatt tcaggagtga
     8881 gggttaggga gaggtttccc ccgttacttt attcgtaccc cttataatgg ggtgttagc
//
LOCUS       DAJTRB010000146         8847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTRB010000146 DAJTRB010000000
VERSION     DAJTRB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.146"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(345..551)
                     /locus_tag="OO330_003815"
     CDS             complement(345..551)
                     /locus_tag="OO330_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5884511.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            864..953
                     /gene="kdpF"
                     /locus_tag="OO330_003816"
     CDS             864..953
                     /gene="kdpF"
                     /locus_tag="OO330_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5884512.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            953..2626
                     /gene="kdpA"
                     /locus_tag="OO330_003817"
     CDS             953..2626
                     /gene="kdpA"
                     /locus_tag="OO330_003817"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5884513.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2649..4696
                     /gene="kdpB"
                     /locus_tag="OO330_003818"
                     /pseudo
     CDS             2649..4696
                     /gene="kdpB"
                     /locus_tag="OO330_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4705..5277
                     /gene="kdpC"
                     /locus_tag="OO330_003819"
     CDS             4705..5277
                     /gene="kdpC"
                     /locus_tag="OO330_003819"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5884514.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5270..7950
                     /gene="kdpD"
                     /locus_tag="OO330_003820"
                     /pseudo
     CDS             5270..7950
                     /gene="kdpD"
                     /locus_tag="OO330_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7947..8624
                     /gene="kdpE"
                     /locus_tag="OO330_003821"
     CDS             7947..8624
                     /gene="kdpE"
                     /locus_tag="OO330_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5884515.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1915 a         2256 c         2524 g         2152 t
ORIGIN      
        1 attgcggtag cgatactaat aacaaaattc ggaatttatt tagatgctgg aattagaaaa
       61 agggatgacg gctttctcac cttaccctga agtttcacga aagtgtgtaa ggaagtatgc
      121 aatctaactg gagatttctg atgatctagt cgcttagagc gacaatgaag gtgaccgcat
      181 cctgggtagc cctatgaaag gatactattg aaacgtgatg ctaaaacagt gatgaaatga
      241 ataatacgat ggaaacgtct tccatgttaa ttatcatgga agacgttgat gttgaacacc
      301 taaaccagcc ccctgatacg aggctggctt aagcgcgcag gcaatcagta aaaatcaccg
      361 gttgcctttt cggcctgatc catccacacc ggtttatcgc tggttttcga ccagacgcgg
      421 tgcaggtagc tgtaaaagcg ggcgcggtct ttccagaaaa gcatgaaagg cagcgccaga
      481 acgcccgctg caaccgcata tgtacggcgt aaaaagataa gccatgcagg atattctctg
      541 tagagttcca tacttttctc ccccttttgt atctacccgg tgaatagcac cggaaaaatg
      601 aatttgttta tctgatgaaa atagtaccgc cttttgtgta attttactac tcatccgacc
      661 acttattttt gcttattgat ggtttattta cattcacact gtaattaaat tacacaaaag
      721 ttaaattaat actaaccatc agttaaatca tggcctttgc catttttata ctttttttac
      781 accccgtccg cagatttttg cgaaatcttt gcagccagaa ttctactctt ccggtatcac
      841 ttttaggcca ctggaggtgc actgtgagtg caggcgtgat agccggcgtg ttgctggtgt
      901 ttttattact gggttatttg gtttatgccc tgatcaatgc ggaggcgttc tgatggctgc
      961 gcaagggttc ttactgattg ccacgttttt actggtatta atggtgctgg cgcgtccttt
     1021 aggcagcggg ctggcgcggc tgattaatga cattcctctt ccaggtacag cgggcgttga
     1081 acgtatactc tttcgtttac ctggcgtctc tgaccatgag atgaactgga agcaatatct
     1141 ttgtgccatt ctcggcctga acatgctggg gctggcggtg ctgtttttta tgttgctcgg
     1201 tcagcactat ctgccgctta atccacagca gttgccgggg ctgtcgtggg atctggcgct
     1261 gaataccgcc gtcagctttg tcacgaatac caactggcaa tcttatagcg gtgaaaccac
     1321 gttgagctat ttcagccaga tggcaggctt aacggtacaa aactttcttt ctgccgccag
     1381 cgggattgcg gtgatttttg ccttcatccg tgcgtttact cgccagagca tgagcacact
     1441 cgggaatgcc tgggtcgatc tgctacgcat cacgttatgg gtgctagtcc ctgtggcgtt
     1501 gttgatcgca ctgtttttta ttcaacaagg tgcgcagcaa aactttctgc cctatcaggc
     1561 agtgaatacc gttgaaggag cgcaacagct gttacccatg ggaccagtag cttctcagga
     1621 agcgatcaag atgctcggta ctaacggcgg tggcttcttt aatgccaact cgtcgcatcc
     1681 gtttgaaaac ccaaccgcac tgaccaactt cgtgcagatg ctggcgatct tcttgatccc
     1741 aacagcgctg tgctttgcct ttggtgaagt gacgggcgat cgccgccagg ggcgcatgtt
     1801 gctgtgggcg atgtcagtga tttttgtcat ctgcgtaggc gtggttatgt gggcagaagt
     1861 tcagggtaat cctcatctgc tggcgctcgg cgcagacagc agcatcaata tggaaggtaa
     1921 agagagccgt ttcggcgtgc tggtcagtag cctgtttgcg gtcgtgacta cggcggcgtc
     1981 ctgtggcgcg gtgattgcga tgcatgattc gtttaccgct ctcggcggca tggtgccgat
     2041 gtggctaatg caaattggtg aagtggtgtt cggcggtgtc ggttctggtc tttacggcat
     2101 gatgctgttt gtcctgctgg cggtgtttat tgccgggctg atgattggtc gtacaccgga
     2161 atatctgggt aaaaaaatag acgtacgcga gatgaaactg actgcactgg cgattctggt
     2221 tactccaaca ctggtactga tgggcgcggc gttggcgatg atgaccgacg ccggacgtag
     2281 cgccatgctt aaccctagcc cgcatggttt tagcgaagtg ctgtacgccg tgtcgtccgc
     2341 cgctaacaac aacggcagcg cctttgccgg attaagcgcc aactctccgt tctggaactg
     2401 tttactggcg ttctgcatgt ttgtcggtcg cttcggggtg attatcccgg tgatggcaat
     2461 tgccggttcg ctggtgagta aaaagagcca acccgccagc tccggcacgc tgccaacgca
     2521 cggcccgctg tttgttggcc tgttaatcgg caccgtgttg ctggttggcg cactgacctt
     2581 tatccctgcc ctggcgcttg gtccggtggc ggaatatctc tcctgatgat attgagtgag
     2641 cactgaatat gagtcgtaaa caactggcgc tattcgaacc aacacttgtc gttcaggcgc
     2701 tgaaagaagc ggtgaaaaaa ttaaacccgc aggcgcaatg gcgcaatccg gtgatgttta
     2761 tcgtctggat cggcagtctg ctgaccacct gtattagcat cgcgatggca agcggtgcga
     2821 tgcctggcaa tgcgctgttt agcgcggcaa ttagcggctg gctgtgggtc accgtactgt
     2881 tcgctaattt cgccgaggcg ctggcagaag gccgcagtaa agcgcaggcc aacagtctta
     2941 aaggggtgaa aaaaactgcc tttgcccgca agctgcgtga gccgaaatat ggcgctgcgg
     3001 cggacaaagt tcctgccgat caactgcgta aaggtgacat cgtgctggta gaagctggcg
     3061 atattatccc ctgcgatggt gaagtcattg aaggcggcgc atcggtcgat gaaagcgcca
     3121 tcaccgggga atcggcaccg gtgatccgtg aatccggcgg cgattttgcc tccgtcaccg
     3181 gcggcacgcg tattctttct gactggctgg tgattgagtg tagcgttaac cccggtgaga
     3241 catttctgga tcggatgatc gcgatggtgg aaggcgcaca gcgacgcaaa acaccgaacg
     3301 agattgccct gactatcctg ctgattgctc tgaccatcgt ctttttactg gcaaccgcca
     3361 cgctgtggcc gttttccgcg tggggcggta atgccgtgag tgtaacggta ctggtggcgc
     3421 tgctggtctg tctgatccca accactattg gtggcctgtt gtcagcgatc ggcgtcgccg
     3481 ggatgagtcg gatgctgggc gcgaatgtga ttgccaccag cggacgtgca gttgaagcgg
     3541 caggtgacgt tgacgttctg ctactggata aaaccggcac catcacactc ggtaaccgtc
     3601 aggcgtcgga gtttatcccc gcgcagggcg tggatgaaaa aacgctggct gacgccgcac
     3661 aactggcttc gctggctgat gaaacgccgg aaggtcgcag tattgtgatc ctcgccaagc
     3721 aacgttttaa cctgcgcgag cgcgatgtgc agtcgctcca tgccaccttt gtaccgttta
     3781 ctgcgcaaag ccggatgagc gggatcaaca tcgacaaccg catgatccgt aaaggttctg
     3841 tcgatgccat tcgccgccat attgaggcta acggcggtca cttccctgcc gatgttgatc
     3901 aaaaagtcga tcaggttgcg cgtcaaggag ccacgctgct ggtggtggtg gaaggttctc
     3961 gtgtgctggg cgttattgcg ctgaaagata tcgtcaaagg cggtattaaa gagcgcttcg
     4021 cccagctgcg caaatgggca ttaaaacggt gatgattacc ggcgataacc gtctgactgc
     4081 cgccgcgata gctgcggaag cgggtgtcga tgattttctc gccgaagcga caccggaggc
     4141 caagctggca ttgatccgtc agtatcaggc ggaaggtcgt ttggtagcga tgaccggcga
     4201 cagcaccaac gatgctacgg cgctggcgca ggcagatgtc gcggtggcga tgaactccgg
     4261 cacccaggcg gctaaagagg cgggcaatat ggtggatctc gactctaacc cgaccaagtt
     4321 gatcgaggtg gtgcacattg gcaaacagat gctgatgacc cgtggctcgc tgaccacctt
     4381 cagcattgcc aacgatgtgg cgaaatactt cgccattatt ccggcggcgt tcgcggcaac
     4441 atatccgcag ttaaatgcgc tgaacatcat gcgcctgcat tcgcccgact ccgccattct
     4501 cagtgcggtc attttcaacg ccttgattat cgtctttttg attcctctgg cgttaaaagg
     4561 cgtgagttat aaaccgctta ccgcttctgc catgttgcgc cgtaacttat ggatttacgg
     4621 tctgggtggg ctgctggtgc cgtttattgg tatcaaagtc attgatttac tgctaaccgt
     4681 ttgtagtctg gtgtgaggtt taccatgagt ggattacgtc cggcattatc gacatttctc
     4741 tttctgttat tgattactgg cggcgtttac ccgctgctga ccaccgcact ggggcaatgg
     4801 tggtatccct ggcaggccaa cggttcgttg attcgtgaag gtgatacggt gcgcggttcg
     4861 gcattaatcg ggcaaaattt taccggcaac ggctattttc atggtcgccc gtcggcaaca
     4921 gcagaaatgc cctataatcc acaggcttct ggcgggagca atctggcagt cagtaacccg
     4981 gagctggata aacagatagc cgcacgcgtt gccgcattac gggccgctaa cccggacgcc
     5041 agcacgaacg ttccggttga actggtgacg gcgtcggcaa gcggactgga caataacatc
     5101 accccgcaag cggcggcctg gcagatccca cgcatcgcaa aagcgcgtaa tctcagcgtt
     5161 gaacagctca cgcagctgat cgcaaaatac agccaacaac cgctggtgaa atatatcggc
     5221 cagccggtcg tcaacattgt cgaactcaat ctggcactgg ataagcttga tgaataacga
     5281 acccttacgt cccgaccccg accgtctgct ggaacaaact gccgcgccgc atcgggggaa
     5341 gctgaaagtt ttcttcagtg cctgtgcagg cgtcgggaag acctgggcga tgctggcaga
     5401 agcccagcga ctgcgggcgc aagggctgga tattgtggtt ggtgtggtag aaacccatgg
     5461 gcggaaagat accgccgcca tgcttgaagg gctggctgtt ctgcctctga aacgccaggc
     5521 gtaccgtggg cggcatatca gcgagtttga tctcgatgcc gccctcgccc gccgtccggc
     5581 gctgatctta atggacgaac tggcgcacag taatgcgcca ggttctcgtc atcctaaacg
     5641 ctggcaggat atcgaagaac tgctggaagc tggcattgat gttttcacaa ccgtcaacgt
     5701 tcagcatctg gaaagtctga atgatgtggt cagcggcgtc accggaattc aggtacggga
     5761 aaccgtgccc gatccttttt tcgatgccgc cgacgacgtg gtgctggtgg acttgccccc
     5821 ggacgatctg cgccagcggc tgaaagaagg caaagtctat attgccgggc aggcggagcg
     5881 cgccattgaa cattttttcc gcaaaggcaa tctgatcgcc ctgcgcgaac tggcactgcg
     5941 ccgtactgcc gatcgcgttg atgagcaaat gcgcgcctgg cgggggcatc ctggcgaaga
     6001 gaaagtctgg cacacgcgcg acgcgatcct tttatgcatc ggccataaca ccggcagcga
     6061 aaactggtcc gcgcggcggc gcggctgacg tcacggctgg ggagcgtctg gcacgcggtg
     6121 tatgttgaaa cccctgccct gcatcgctta ccggagaaaa aacgtcgggc aattctcagc
     6181 gccttacgtc tggcgcagga gctgggcgcg gagacggcaa cactttctga tccagcggaa
     6241 gagaaagcgg tagtgcgtta tgcccgtgaa cataatctcg gcaagattat tctcggtcgc
     6301 ccagcctcgc gccgctggtg gcgtcgggaa acatttgctg accgactggc gcgcatcgcc
     6361 cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac ccgcccgcac gattaacaac
     6421 gcgccggata gccgctcatt taaagacaag tggcgggtac agattcaggg atgcgtggtt
     6481 gccgccgcgt tatgcgccgt tatcacctta attgccatgc agtggttgat ggcgtttgat
     6541 gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg tggcgctatt ttatggacgc
     6601 tggccttcgg tggttgccac cgtcattaat gtggtgagtt tcgatctctt ttttatcgcc
     6661 ccacgcggca cgctcgccgt ctctgatgtg caatatctgc tgaccttcgc ggtgatgtta
     6721 accgtcgggc tggtgatcgg gaaccttact gctggcgtgc gttatcaggc gcgggtagcc
     6781 cgttaccgcg agcaacgcac acggcactta tatgaaatgt cgaaagctct ggcggtgggc
     6841 cgcagtccgc aggacatcgc cgccaccagc gaacaattta ttgcctccac gtttcatgcc
     6901 cgcagtcagg tgttgttgcc cgatgacaac ggtaaattgc agccgttaac acatccgcaa
     6961 ggagttacgc catgggacga tgccatcgcg cagtggagtt ttgataaagg cctgcctgcg
     7021 ggcgcgggca ccgacacgtt acccggtgta ccgtaccaga ttttgccgct aaaaagcggc
     7081 gagaaaacct acgggctggt ggtggtggag ccggggaatc tgcgccagtt gatgatcccg
     7141 gaacagcagc gcctgctgga gacttttacg ctgttagtcg ccaatgccct tgagcggctg
     7201 acgctaaccg ccagcgaaga acaggcgcgg atggcaagcg aacgtgaaca gatccgcaac
     7261 gccctgctgg cagcgctttc gcatgattta cgcacgccgc ttacggtgct gtttggtcag
     7321 gcagaaatct taacgctcga tctggcaagc gaaggatcac cccacgcccg ccaggccagc
     7381 gagattcgtc agcatgtgct gaacactacc cgactggtga ataatctact ggatatggcg
     7441 cgaattcagt ccggcggctt taatttgaag aaagagtggt taacgctgga agaagtagtc
     7501 ggcagcgcgc tgcaaatgcc ggaaccgggt ttatcgtcgc ccatcaatct ttctctgcca
     7561 gaaccgctga ccttaatcca cgttgacggg ctactctttg aacgggtgct gattaatctg
     7621 ctggagaacg cggtgaaata tgcgggtgcg caggccgaaa ttggtatcga tgcccacgtt
     7681 gagggcgaaa atctacaact ggatgtctgg gataacggcc ccggtcttcc gccaggccag
     7741 gagcagacga tatttgataa gtttactcgc gggaataaag agtcggcggt gccgggtgtc
     7801 gggcttgggt tggcaatttg tcgggcgatt gtggatgtac acggcggcac cattaccgcg
     7861 ttcaaccgac cggaaggtgg tgcctgtttt cgtgttacac ttccccagca aaccgctcct
     7921 gaacttgaag actttcatga ggatatgtga caaacgttct gattgttgaa gatgaacagg
     7981 ctattcgtcg ctttctgcgc acggcgctgg agggcgacgg gatgcgcgtc tttgaggccg
     8041 aaactctgca acgcggcttg ctggaagcgg caacccgtaa gccagatttg attattctcg
     8101 atctcggcct gcccgatggt gatgggattg agtttatccg cgacctgcgc cagtggagcc
     8161 cggtgccggt gattgtgctt tccgcacgca gcgaagagag cgacaagatc gccgcgctgg
     8221 atgccggagc ggatgattat ctgagtaagc cgtttggtat tggcgaattg caggcccgtc
     8281 tgcgcgtcgc tttacgccgc cactccgcca ccgccgcgcc cgatccgctg gtgaaatttt
     8341 ccggtgttac cgtcgattta gccgcccgcg tgattcaccg gggtgaggaa gaggtgcatc
     8401 tcacaccaat tgagttccgc ctgctggcgg tgctgctcaa caatgccgga aaagtactca
     8461 cccagcgcca gctccttaac caggtgtggg ggccaaacgc ggtcgaacac agtcactatt
     8521 tgcgtattta tatgggacat ctgcgacaaa aactggaaca ggatccggcc cggcctcgcc
     8581 acttcattac tgaaaccggt attgggtatc ggtttatgct ttgaattgtt attcaataaa
     8641 ttcgcttaaa tagtgattaa tttattatta aacctgcgcc gaatattatt atttggcagg
     8701 tttaatttct taacgcaaat ataaaacacg aaattacaac atttaagtaa caaaacagca
     8761 tctaatatgg cgatattcat aattaatgat ttattatctg gggtatgatc gttttttgtt
     8821 gatcttcttc acagattata gccattt
//
LOCUS       DAJTRB010000147         8762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTRB010000147 DAJTRB010000000
VERSION     DAJTRB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.147"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            5..436
                     /locus_tag="OO330_003822"
     CDS             5..436
                     /locus_tag="OO330_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5884516.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO330_003823"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OO330_003823"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5884517.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO330_003824"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OO330_003824"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5884518.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO330_003825"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OO330_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5884519.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OO330_003826"
     CDS             complement(4438..5145)
                     /locus_tag="OO330_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5884520.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO330_003827"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OO330_003827"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5884521.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OO330_003828"
     CDS             complement(6003..6206)
                     /locus_tag="OO330_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5884522.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="OO330_003829"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="OO330_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8762
                     /locus_tag="OO330_003830"
     CDS             7870..>8762
                     /locus_tag="OO330_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5884523.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         2033 a         2389 c         2251 g         2089 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtga
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgcgaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacctgaa
     8761 ca
//
LOCUS       DAJTRB010000148         8671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTRB010000148 DAJTRB010000000
VERSION     DAJTRB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.148"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OO330_003831"
     CDS             complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OO330_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5884524.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1968..3209)
                     /gene="hemX"
                     /locus_tag="OO330_003832"
     CDS             complement(1968..3209)
                     /gene="hemX"
                     /locus_tag="OO330_003832"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5884525.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPQGDTPAAAPQGE"
     gene            complement(3231..3971)
                     /gene="hemD"
                     /locus_tag="OO330_003833"
     CDS             complement(3231..3971)
                     /gene="hemD"
                     /locus_tag="OO330_003833"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5884526.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3968..4909)
                     /gene="hemC"
                     /locus_tag="OO330_003834"
     CDS             complement(3968..4909)
                     /gene="hemC"
                     /locus_tag="OO330_003834"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5884527.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5296..7842
                     /gene="cyaA"
                     /locus_tag="OO330_003835"
     CDS             5296..7842
                     /gene="cyaA"
                     /locus_tag="OO330_003835"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5884528.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7882..8202)
                     /gene="cyaY"
                     /locus_tag="OO330_003836"
     CDS             complement(7882..8202)
                     /gene="cyaY"
                     /locus_tag="OO330_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5884529.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8250..>8671)
                     /locus_tag="OO330_003837"
     CDS             complement(8250..>8671)
                     /locus_tag="OO330_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5884530.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1973 a         2238 c         2236 g         2224 t
ORIGIN      
        1 agttgacggg gtatctgctg cgctggccat gagaggaaga gcggcggcga cagcaacaac
       61 aagacgtttg ggtgaaaacg aaaattccat gcaaaatgct ccggtttcat gtcatcaaaa
      121 tgatgacgta attaagcatt gataattgag atccctctcc ctgacaggat gattacataa
      181 ataatagtga caaaaataaa ttatttattt atccagaaaa tgaattggaa aatcaggaga
      241 gcgttttcaa tcctacctct ggcgcagttg atatgtaagg caggtttatt atatcgcgtt
      301 gattattgat gctgttttta gttttaacgg caattaatat atgtgttatt aattgaatga
      361 attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat tcactcagga
      421 agttattact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      481 gttatttccc gattctctgt ggcataataa acgagtagat gctcattcca tctcttatgt
      541 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      601 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      661 cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      721 tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg agaaggaact actgtggcgg
      781 gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      841 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      901 cagcgccgcg cggaaggcga gcgatgcttc ctgccattca ccgtgcttca tcagtgactg
      961 acccaatgtg ctccacaaca gcgggcgatc accgacgttt ttgatttgct ggcgtagcac
     1021 tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca gtaggcgatc
     1081 gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat gatcgtcaca
     1141 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
     1201 ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg cctgatccat
     1261 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
     1321 tttcgccatt gatgggatga tatccagcag agaactccat gcccctgtgc ggatatacgc
     1381 ctgttccgcc agacgtaata cttcaggatg gcgtggcgta acttccagca gcttatccac
     1441 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1501 taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg
     1561 tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1621 ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat caccttccgc
     1681 cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg cgacgcttac gtccgacaaa
     1741 ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgt agtagccact caatggcaaa
     1801 cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg tttcgatatt
     1861 gtagttgtcg gtctggatca gcacataacc ctgatgaccg gcaatcatcg ggccaaccac
     1921 tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta ttctccttgc
     1981 ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc aggtgcaggt
     2041 gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtttagcttc cgttgtcccc
     2101 gctgccggtt gtgccagcag gttacgcacg cgagtctgca tcagtttttc cagcatcgcc
     2161 tggctttgca gggtttccgg aagatccatc gagatatttt gctggcttaa ctggtccacc
     2221 tcgtcgagga acgctttggt ggtggcatca tcagtatcgt agtaagcacg tacccaggtg
     2281 gagacgttct ccagcgcctg gcgataagtc tcttcctgat gacgcggtac agcttgtgct
     2341 gcgaccagca ggcgagagcg aatattttcg cgcagataga tatcctgatt tggcgctaac
     2401 agcggtacgg cggtgtcatc acgacggcga atcgtaatga agttgtccat aaagttctgc
     2461 cagctttttt gcagattgat acgccattcg ctgatggaac tggaaagctc ttcaccgtct
     2521 gaatccatcg gcgaaccatc gctgtcatta tcggccagac gcaggttatc tacctgattt
     2581 gaaagctgat taagcttaag gatgatgccg tcataatcca cctgcgatac tgcagaaagg
     2641 ctggcgatat catcggtaat tgcccgacga acggtaatca gactcgggtc attcatatcc
     2701 gccaggctgg cgtctgcact tttcagcaac gctgcagagg tcgtgacgtc ctgatcgctc
     2761 cacagcttcc gtccggcgag tttcaccaga aaatcggcct gagccagcag ccaggtttta
     2821 gcatcgctgc cggaaatggt ggcgatcttt tgttggactt catccaactg ttttgccagc
     2881 gtttcttgct gacgattcgc ctgcttaagc tgcgcagctt gttgcttaat aatgccttcc
     2941 agctcggctt tttggctctc ctgggctttt tgcaatgccg tcagttggtt agccagggca
     3001 tcgctggtgg cggtctgatt gacggcctgt tgtttacccc agccatacaa accgacaccc
     3061 gccgccagag caatagcgat agccaccgcg ctgagaatca atgcagtatt gttcttactc
     3121 tttttttctg ttgcgacagg ttgtgacgtg gtgtccacgg cctccctggt ctcttcaacc
     3181 acggcggagg ttttttcttg ttccgtcatt atggcttcct gttatgagag ttattgtaat
     3241 gcccgtaaaa gcgcatcgtt gtcagcgtta tcggcgacct taatgtcttg ccagcccagt
     3301 tcccgggcga gtttcgccaa acgctcactg acgaccaata gtcgacagtg tagtaaccag
     3361 tgctcacgat accattgtgg gatcagcgac cagagttgct gcaacatttc accgctggta
     3421 acaacgacca tcgtcacctc gcgggattgc cagcgcatcg cttcttctgc accatcgtaa
     3481 tggattgcgc atcgttgata acattcacaa aaagtgacct cagcaccgcg caccgtcagg
     3541 gtatccccaa ttagctcacg accgccattg ccacgtaata tcagcgcacg tttgcccgca
     3601 atattttgta attcaggtaa ttgtagcaag acttcgctga tttcccgatc ctgtgggtag
     3661 agaatcttct gtccgcttac ggtatgtagc gccagtgcgg tggtgcgtcc aatggcgaaa
     3721 taagtaggta gttggggcca tttacgatct tgctgatgca gctgtgattg ggcaaaagca
     3781 accgcgtgtt gcgagagggc aaacaacaga tcgctctccc ccagcgctgc cagttgatca
     3841 gcaagttgcg gtaattgtcg acccggagaa aactcaatca gcggaaaatg ccaggccacc
     3901 tgccccagtg tgcgcagacg gctcactaac tcttctccag cgggagacgg gcgggtgacc
     3961 aggatactca tgccggggcc tctccgttat agacttcagc gaggatctcg cgcgcgccgt
     4021 tattcagtag ctcttctgcc agcgaaatcc ccatttgttc ggcatcttgc ggcgcaccgc
     4081 ggcgttcacc gcgaataatc tgcgaaccgt ccggcgcgcc gaccagcgca catagccaga
     4141 tttcgccatc aataagctcg gcgtagctac caattggcac ctgacatccg ccttcgagac
     4201 gggtattcat ggcgcgttct gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg
     4261 caagcagctc gcgagtgcgt gtatcatcaa ggcggcattc aataccgact gcaccttgtc
     4321 ctaccgccgg aagagaaatc tcgggtggta acgcggcgcg aatgcgggac tccagaccta
     4381 aacgttttag tccggctacg gcaagaatga tggcatcgta ttcgccgtta tctagtttgc
     4441 tcaggcgagt gccgacgttg ccgcgcaggg agcggataat cagatccggg cggcgttcag
     4501 ccagttggca ctggcgacgt aaactggacg tcccgacgat actgcctgct ggtaacgcat
     4561 ccagactgtc atagttattg gacacaaagg catcgcgagg atcttcacgc tcgcaaatag
     4621 tgaccagccc cagaccttgc gggaattcaa ccggcacatc tttcattgaa tgtacggcga
     4681 tatcagcgcg attctccagg agcgcgactt ccagctcttt tacaaataag ccttttccgc
     4741 ctactttcgc cagcggcgta tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt
     4801 caacgaccag gcccggatgg ctcgccatca acttgtcttt gacatagtgt gcctgccaga
     4861 gtgcaagtgg gctttggcgt gtggcaattc ttaaaacatt gtctaacatg cttgttaccg
     4921 tcattatcat ccgtggtcca tcctaacatc cttgccagag tgatgtcagt gttgtggtga
     4981 aacgtagacg cctgcgcaaa ccgtaaaatg aggtctggca gtggatcctg acaggcgttt
     5041 cacgccgtcg taataaggaa tttacagaga ataaacggtg ctacacttgt atgtagcgca
     5101 tctttcttta cggtcaatca gcaaggtgtt aaattgatca cgttttagac cattttttcg
     5161 tcgtgaaact aaaaaaacca ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat
     5221 atcccgaatg ccgcgtgtta ccgttgatgt tggcggaatc acagtcatga cgggtagcaa
     5281 atcaggcgat acgtcttgta cctctatatt gagactctga aacagagact ggatgccata
     5341 aatcaattgc gtgtggatcg cgcgcttgct gctatggggc ctgcattcca acaggtctac
     5401 agtctactgc cgacattgtt gcactatcac catccgctaa tgccgggtta ccttgatggt
     5461 aacgttccca aaggcatttg cctttacacg cctgatgaaa ctcaacgcca ctacctgaac
     5521 gagcttgaac tgtatcgtgg aatgtcagta caggatccac cgaaaggtga gcttccaatt
     5581 actggtgtat acaccatggg cagcacctcg tccgtagggc aaagttgttc ctctgacctg
     5641 gatatctggg tctgtcatca atcctggctc gatagcgaag agcgccaatt actacaacgt
     5701 aaatgcagcc tgctggaaag ctgggccgcc tcgctgggtg tggaagtcag cttcttcctg
     5761 attgatgaaa accgcttccg tcataatgaa agcggcagcc tggggggcga agattgtggc
     5821 tccacccagc atatactgct gcttgacgaa ttttatcgta ctgccgtgcg tctcgccggt
     5881 aagcgtattc tgtggaatat ggtgccgtgc gacgaagaag agcattacga cgactatgtg
     5941 atgacgcttt acgcgcaggg cgtgctgacg ccaaatgaat ggctggatct cggtggctta
     6001 agctcgcttt ctgctgaaga gtactttggt gccagccttt ggcagctcta caagagtatc
     6061 gattccccat acaaagcggt gctgaaaaca ctgctgctgg aagcctattc ctgggaatac
     6121 ccgaacccac gtctgctggc gaaagatatc aaacagcgtt tgcacgacgg cgagattgta
     6181 tcgtttggcc tcgatccata ctgcatgatg ctggagcgtg ttactgaata cctgacggcg
     6241 attgaagatt tcacccgtct ggatttagta cgtcgctgct tctatttaaa agtgtgcgaa
     6301 aaactcagcc gtgaacgcgc ctgtgtaggc tggcgtcgcg cagtgttgag ccagttagtg
     6361 agcgagtggg gttgggacga agctcgtctg gcaatgctcg ataaccgtgc taactggaag
     6421 attgatcagg tgcgtgaggc gcacaacgag ctgctcgacg cgatgatgca gagctaccgt
     6481 aatctgatcc gctttgcgcg tcgcaataac cttagcgtct ccgccagtcc gcaggatatc
     6541 ggcgtactga cgcgtaaact gtatgccgcg tttgaagcat tgccaggtaa agtgacgctg
     6601 gtaaacccgc agatttcacc cgatctctcg gaaccgaatc tgacctttat ttatgtgccg
     6661 ccgggccggg ctaaccgttc aggttggtat ctgtataacc gcgcgccaaa tattgagtcg
     6721 atcatcagcc atcagccgct ggaatataac cgttacctga ataaactggt ggcgtgggca
     6781 tggtttaacg gcctgctgac ctcgcgcacc cgtttgtata ttaaaggtaa cggcattgtc
     6841 gatttgccta agttgcagga gatggtcgcc gacgtgtcgc accatttccc gctgcgctta
     6901 cctgcaccga caccgaaggc gctctacagc ccgtgtgaga ttcgccatct ggcgattatc
     6961 gttaacctgg aatatgaccc gacagctgcg ttccgcaatc aggtggtgca ttttgatttc
     7021 cgtaagctgg atgtcttcag ctttggcgag aatcaaaatt gcctggtagg tagcgttgac
     7081 ctgctgtacc gcaactcgtg gaacgaagtg cgtacgctgc acttcaacgg cgagcaatcg
     7141 atgatcgaag ccctgaaaac tattctcggc aaaatgcatc aggacgccgc accgccagat
     7201 agcgtggaag tcttctgtta tagccagcat ctgcgcggct taatccgtac tcgcgtgcag
     7261 caactggttt ctgagtgtat tgaactgcgc ctttccagca cccgccagga aaccgggcgt
     7321 ttcaaggcgc tgcgcgtttc tggtcaaacc tggggcttgt tcttcgaacg cctgaatgta
     7381 tcggtacaga agctggagaa cgccatcgag ttttatggcg ctatttcgca taacaaactg
     7441 cacggcttgt cagtgcaggt tgaaaccaat cacgtcaaat taccggcggt ggtggacggc
     7501 tttgccagcg aagggatcat ccagttcttt ttcgaagaaa cgcaagacga gaatggcttt
     7561 aatatctaca ttctcgacga aagcaaccgg gttgaggtgt atcaccactg cgaaggcagc
     7621 aaagaggagc tggtacgtga cgtcagtcgc ttctactcgt catcgcacga ccgcttcacc
     7681 tacggctcaa gcttcatcaa cttcaacctg ccgcagttct atcagattgt gaaggttgat
     7741 ggtcgcgaac aggtgattcc gttccgaaca aaatcgattg gcaacctgcc gcccgccaat
     7801 caggatcacg atacgccgct attacagcag tatttttcgt gatgagcgtg ccggaaagcg
     7861 aggcttatcc ggcatgcaat cttagcggaa actgactgtt tcacccgcct gttgcgtcgc
     7921 cgcctgttcc agcaaatccc agaaggtttc gccgctgcga tcgcaaatcc attcatcgcc
     7981 tttcaggtca aaatggtagc cgccctgttt ggttgccagc cacacctggt gcagcggctc
     8041 ctggcggtta ataatgattt tgctgccgtt ttcaaaggta atggtcagta cgccgccgtt
     8101 gatttcgcag tcgatatcgc tgtcgccatc cctgtcgtcc agacgctctt caatggtcag
     8161 ccacagttga tcagccaggc gatgaaattc actgtcgttc attgttgtat cctgttttta
     8221 agtgatggcg gcagtatagc ggcatggggt cagggcttca aagtttgcac ctctgctgct
     8281 gcgttccggc atgattcatc cgtcaccgga ataatgatgt ctctgttttg aacttcacgt
     8341 acgttctcct ctctgacacg agttttcgcc gggcaggggg agggcgtaat ccttacctct
     8401 gtatcggtcc atgtataaac attgcgatcg tcataagcga agcgatcgtt tttagttagc
     8461 gaaccgttaa ctggaataat ttgcaagctc tttgaccgta attggcggat aaagtaagca
     8521 tgctgtttgc cgctattaaa tgagcgatca atgaccaggt ctggcgtatc cagcgggatt
     8581 atctcaagct ggtcttcacc aaatgtcgat aattttgtta catacagccg atctttatat
     8641 tgcgcaatat tgccagtaat cagatggaat t
//
LOCUS       DAJTRB010000149         8330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTRB010000149 DAJTRB010000000
VERSION     DAJTRB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.149"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..159
                     /locus_tag="OO330_003838"
     CDS             <1..159
                     /locus_tag="OO330_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884531.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWLQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO330_003839"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO330_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5884532.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO330_003840"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO330_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5884533.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="OO330_003841"
     CDS             875..1246
                     /locus_tag="OO330_003841"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5884534.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKCL
                     IEAHNQAVDFAVRQVEALAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="OO330_003842"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="OO330_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="OO330_003843"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="OO330_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5884535.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="OO330_003844"
     CDS             6949..7509
                     /locus_tag="OO330_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5884536.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="OO330_003845"
     CDS             7640..7852
                     /locus_tag="OO330_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5884537.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8330
                     /locus_tag="OO330_003846"
     CDS             8096..>8330
                     /locus_tag="OO330_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5884538.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTV"
BASE COUNT         1913 a         2082 c         2672 g         1663 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggctgca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcctgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaatgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc actggcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcacggcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aacgccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagaagccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctagccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccttgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggtat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca
//
LOCUS       DAJTRB010000150         8230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTRB010000150 DAJTRB010000000
VERSION     DAJTRB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.150"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..86)
                     /locus_tag="OO330_003847"
                     /pseudo
     CDS             complement(1..86)
                     /locus_tag="OO330_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            84..408
                     /locus_tag="OO330_003848"
                     /pseudo
     CDS             84..408
                     /locus_tag="OO330_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(438..3350)
                     /locus_tag="OO330_003849"
     CDS             complement(438..3350)
                     /locus_tag="OO330_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884539.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3353..3469
                     /locus_tag="OO330_003850"
     CDS             3353..3469
                     /locus_tag="OO330_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5884540.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3833..4675
                     /locus_tag="OO330_003851"
     CDS             3833..4675
                     /locus_tag="OO330_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5884541.1"
                     /translation="MLNEGGILFKANHIPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4678..5766
                     /gene="capU"
                     /locus_tag="OO330_003852"
     CDS             4678..5766
                     /gene="capU"
                     /locus_tag="OO330_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5884542.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5771..6721
                     /gene="virK"
                     /locus_tag="OO330_003853"
     CDS             5771..6721
                     /gene="virK"
                     /locus_tag="OO330_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5884543.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6786..7730
                     /gene="lpxM"
                     /locus_tag="OO330_003854"
                     /gene_synonym="msbB"
     CDS             6786..7730
                     /gene="lpxM"
                     /locus_tag="OO330_003854"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5884544.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7884..>8230)
                     /locus_tag="OO330_003855"
     CDS             complement(7884..>8230)
                     /locus_tag="OO330_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5884545.1"
                     /translation="GGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQS
                     WLKENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVS
                     PFPGGKHGLAKV"
BASE COUNT         2193 a         1859 c         1950 g         2228 t
ORIGIN      
        1 tcctgctccg acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg
       61 aacagtccac aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc
      121 agttccgtca gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca
      181 cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca
      241 agcctgcgcc gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac
      301 cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
      361 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat
      421 cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat
      481 ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat
      541 tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc
      601 aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg gttaagaata
      661 tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact
      721 tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaac
      781 gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa
      841 aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt
      901 gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca
      961 ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc
     1021 gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggatct
     1081 gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc
     1141 agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac
     1201 acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag taactgcaga
     1261 gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc
     1321 gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct
     1381 gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat
     1441 ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc
     1501 tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc
     1561 agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc
     1621 tgctgttcaa caagatacac attgtgtgta gcacgggtga gcgcaacata cagcgcgttg
     1681 gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg
     1741 gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg
     1801 gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga
     1861 accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc
     1921 atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc
     1981 tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct tctgacaaaa
     2041 tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta
     2101 gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg
     2161 tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc
     2221 ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt
     2281 ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca
     2341 tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga
     2401 tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc
     2461 tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc
     2521 ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca
     2581 gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc
     2641 acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca
     2701 atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc
     2761 agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt
     2821 accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg
     2881 tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca
     2941 tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt
     3001 tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta
     3061 taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac
     3121 agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt
     3181 ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa
     3241 cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc
     3301 cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg
     3361 aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga
     3421 aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg
     3481 tcatgatgcc ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa
     3541 ctctgcgctg aaattccttt ttttcattgg agcacctgtg ttgttctgag gtgaacatat
     3601 cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt
     3661 taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat
     3721 atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca
     3781 gctttattgg gttttccttt tgtctctgct tactaggtgc cagaaacttt aaatgttaaa
     3841 cgaagggggc attttgttca aagccaatca tattccggta ttaatgtatc atcatgtcag
     3901 tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt
     3961 ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggcgg
     4021 aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt
     4081 tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt taattaccgg
     4141 ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg
     4201 cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt ctgaagtaaa
     4261 cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc atacacgctg
     4321 ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg acttgttgag
     4381 cgggagagaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt gttggccgga
     4441 aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata
     4501 tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag
     4561 caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacccc
     4621 gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg
     4681 aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct
     4741 caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc
     4801 aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt atttcgaaac
     4861 agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat
     4921 gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact
     4981 aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt
     5041 ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat
     5101 ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag
     5161 aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc
     5221 cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa
     5281 tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt
     5341 gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc
     5401 atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt
     5461 gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca
     5521 tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag
     5581 aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg
     5641 aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat
     5701 atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac
     5761 aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt
     5821 tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat
     5881 tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt
     5941 gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc
     6001 ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg
     6061 ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc
     6121 attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg
     6181 ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag tgattgcccg
     6241 gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca
     6301 gggagccccc aaaaataccg gacctgatgt tattcggtgc gcaacccggg cctgttatgg
     6361 gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt
     6421 ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta
     6481 tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa
     6541 aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat
     6601 agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt atatccatga
     6661 aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta
     6721 aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact
     6781 aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc
     6841 ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc
     6901 cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa
     6961 aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag
     7021 aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg
     7081 cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg
     7141 aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt ccccatggct
     7201 ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt
     7261 ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg
     7321 gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg
     7381 gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg
     7441 atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc
     7501 aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg
     7561 aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg attgcccgac
     7621 aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt
     7681 taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag
     7741 tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg
     7801 tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatgc
     7861 ctaaatagct gcgcttaata ccgctacact tttgccagcc catgtttgcc tccggggaat
     7921 gggctgacgg tttccataaa atggcgaact tttttcaaca gttgccacat tgagcggcac
     7981 tgatgattac gcgttattgt gtcgtgaagt gcctgccata gccgttcaac atgattcacc
     8041 catggcgagt aaaccggctg ataaatgccc ctgaacttcg ggttctcctt cagccagctc
     8101 tgtgtttccc ggcttttgtg gataatgtag ttgtccacga tcagcgtgat ggttttcgca
     8161 cgacggtatg tcgctttaag ccgcttcagc aggctgatga acagcgccga acttttgctg
     8221 ttgccgccca
//
LOCUS       DAJTRB010000151         8229 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTRB010000151 DAJTRB010000000
VERSION     DAJTRB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.151"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..695
                     /locus_tag="OO330_003856"
     CDS             <1..695
                     /locus_tag="OO330_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5884546.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1034..1804)
                     /locus_tag="OO330_003857"
     CDS             complement(1034..1804)
                     /locus_tag="OO330_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884547.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="OO330_003858"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="OO330_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="OO330_003859"
     CDS             complement(2295..2516)
                     /locus_tag="OO330_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884548.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="OO330_003860"
     CDS             complement(2517..3200)
                     /locus_tag="OO330_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5884549.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="OO330_003861"
     CDS             complement(3264..3575)
                     /locus_tag="OO330_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884550.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3572..4474)
                     /locus_tag="OO330_003862"
     CDS             complement(3572..4474)
                     /locus_tag="OO330_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5884551.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="OO330_003863"
     CDS             4914..5873
                     /locus_tag="OO330_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5884552.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="OO330_003864"
     CDS             5873..6268
                     /locus_tag="OO330_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5884553.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="OO330_003865"
     CDS             6451..6831
                     /locus_tag="OO330_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5884554.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6877..7721
                     /locus_tag="OO330_003866"
     CDS             join(6877..7219,7219..7721)
                     /locus_tag="OO330_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5884555.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSSAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATALD
                     GSNIRALKCAAGGSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAH
                     ESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNE
                     KMASDGRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFY
                     QRLRN"
     gene            7836..>8229
                     /locus_tag="OO330_003867"
     CDS             7836..>8229
                     /locus_tag="OO330_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5884556.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGT"
BASE COUNT         2230 a         2125 c         2023 g         1851 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca tcgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggttca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaaat agtatccgtc tcagttcgtg gtgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 cataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct
     6061 aatcagcgtt tttttcagtg agaaaattac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcgaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga
     6961 cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttccagt
     7021 gcaccatggc gcgatttacc cgaacgatat ggggcatgga aaactgttta taaccgcttt
     7081 aaccgatggt caaagtcagg cgtgattaat attattttca acaggttgct ttcgctactt
     7141 gatgcaaacg gctttattga ctggtctgcc accgcgctgg atggcagtaa tatccgggcg
     7201 ctgaaatgtg ccgccggtgc tcaaaaaaac atcccgatat cgacggagat aatgggccgg
     7261 gtcgctctcg cggcggtttt ggcaccaaaa tccatctggc aacagacgga agtggcctcc
     7321 cgttaaatat cgtgctaagc cccggacaag ctcatgaaag ccagttcgcg caacgacttc
     7381 tggacgggat tggcgttcag cgtcagaacg gcagcatgaa acgccgtgga catgcggtac
     7441 tggctgataa agcgtattca gggcgcgcat tgcgcaacga gctgaaaaat aacggtataa
     7501 aggcagtaat cccccgaaag tcaaatgaga aaatggcatc ggatggacgt gcacagcttg
     7561 atcgtgatgc gtactgcaat cgtaatgtcg ttgaacggtg ctttgggcgg ctgaaagaat
     7621 atcgccgcat cgccacgcgt tacgacaaaa cggcgagaaa ttacctggcg atggtgaaac
     7681 tgggctgcat ccgactcttt tatcaacgct tacgtaatta agggacacag cctaaatagc
     7741 tgcgcggaat agtagatccc tttgagggaa cttagcccgg attgtgcgat ctgatcaatc
     7801 gccaaatcaa aacaaatcac caaccggact gagcaatgcc gatcatagca ccaatttccc
     7861 gtgacgaacg acgcctgatg cagaaagcca tccataaaac acacgataaa aattatgccc
     7921 gcagactgac tgccatgctg atgctgcacc ggggcgaccg tgtcagcgac gttgccagaa
     7981 cgctctgctg cgcccgttcc tctgttggac gctggattaa ctggttcacg cagtcgggtg
     8041 ttgagggact gaaatcatta cctgccgggc gtgcccgtcg ctggccgttt gagcatatct
     8101 gcacactgtt acgtgagctg gtaaaacatt ctcccggcga ctttggctac cagcgttcac
     8161 gctggagtac agaactgctg gcaataaaaa tcaatgagat aaccggttgc cagttaaatg
     8221 ccggaaccg
//
LOCUS       DAJTRB010000152         7956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTRB010000152 DAJTRB010000000
VERSION     DAJTRB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.152"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..131)
                     /locus_tag="OO330_003868"
     CDS             complement(<1..131)
                     /locus_tag="OO330_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5884557.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     "
     gene            complement(218..579)
                     /locus_tag="OO330_003869"
                     /pseudo
     CDS             complement(218..579)
                     /locus_tag="OO330_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(918..1472)
                     /locus_tag="OO330_003870"
                     /pseudo
     CDS             complement(918..1472)
                     /locus_tag="OO330_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1615..1737
                     /locus_tag="OO330_003871"
                     /pseudo
     CDS             1615..1737
                     /locus_tag="OO330_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1999..2565)
                     /gene="ipgB2"
                     /locus_tag="OO330_003872"
     CDS             complement(1999..2565)
                     /gene="ipgB2"
                     /locus_tag="OO330_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5884558.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2847..3161)
                     /locus_tag="OO330_003873"
     CDS             complement(2847..3161)
                     /locus_tag="OO330_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884559.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RL"
     gene            complement(3239..3349)
                     /locus_tag="OO330_003874"
                     /pseudo
     CDS             complement(3239..3349)
                     /locus_tag="OO330_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3673..4350)
                     /gene="ospD1"
                     /locus_tag="OO330_003875"
     CDS             complement(3673..4350)
                     /gene="ospD1"
                     /locus_tag="OO330_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5884560.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4721..4858)
                     /locus_tag="OO330_003876"
                     /pseudo
     CDS             complement(4721..4858)
                     /locus_tag="OO330_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4940..5238)
                     /locus_tag="OO330_003877"
                     /pseudo
     CDS             complement(4940..5238)
                     /locus_tag="OO330_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5827..5986)
                     /locus_tag="OO330_003878"
                     /pseudo
     CDS             complement(5827..5986)
                     /locus_tag="OO330_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5990..7219)
                     /locus_tag="OO330_003879"
                     /pseudo
     CDS             complement(5990..7219)
                     /locus_tag="OO330_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7239..7586)
                     /gene="tnpB"
                     /locus_tag="OO330_003880"
     CDS             complement(7239..7586)
                     /gene="tnpB"
                     /locus_tag="OO330_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5884561.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7586..>7956)
                     /locus_tag="OO330_003881"
     CDS             complement(7586..>7956)
                     /locus_tag="OO330_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5884562.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
BASE COUNT         2038 a         1859 c         1741 g         2318 t
ORIGIN      
        1 ggctcagatt ttgcctgcaa ataacgttta acggtattgc gggagagccc cagttctctg
       61 gcaatcgccc ggctactcat tccctgcttg tgcaggattt taatttccat aactgtctca
      121 aaagtgacca taagctctcc tgaatcagga gagcagatta ccccctggat ctgatttcag
      181 gcgttgggtg tggatcacta ttgcaccgtt cgtgacagtg ttgttgcgcc ttgctgccaa
      241 cgaagctcca ctgcccatca agttcacaga taagggcaac atcagcgtga gcgactggcg
      301 cagatgttat ccgccggggc gggagctttt taatgtccgt atgacggtat tgatgccgat
      361 tttcagcgta cggccagtat cgcgaactcc ggcaccgttg aaagccattt cagtgatttg
      421 gtttttaact cccggtttac gggcctcgta acagtaagtg agctgaaaca cccggtgaca
      481 gtcacggcaa cggaatcgat catgtccttt tgggttctgc ccgtgacggt aaacctgaac
      541 ggactggcaa cgggggcaat gaaagttaac tgtgaccatg aaagaaccta aaagcgggta
      601 ttataacccc taattcaact aattggaggc attgtcattt ttaatctatg gatacactta
      661 aaaatcctct caccttacgt taaaaacctt ctcatgttta gagtttttta ttatgtttat
      721 cataatgata tgacgatctg agtttttaaa atcaagaaga aaagataagt tttttaaatg
      781 cacagtgcct gtggataact ttgcgtaaat ccggaggaca gatcactctg aagcaagaca
      841 acagcatctg aaaacaatat gctcacgttt caaccacagg gcgtaagtga tcctacccac
      901 gtaatatgga cacaggccta agcgaggttc tggttttcaa attgttccgg actgaggccg
      961 ccacaccaac tgtgccgtcg ccagcgattg taatcacatt cgatataatt aaacactgtt
     1021 gcccgcatta tttcccggct gataaagtgt tctccgtgga gacattccac tttcagcgaa
     1081 tgaaagaagc tttccacgca ggcattatcg tagcagcagc cctttgcgct catactgcca
     1141 cgcagattat gccacttcag cagcgcctga taatctgctg aacagtactg acttcctcgg
     1201 tccgagtgaa cgataacgtt ccggggtctc ttacgccgcc acagcgccat ctgcagggca
     1261 tcgcaggcca gttgcgccgt caggcgtggt gacatggacc agccaataac ggcacgtgac
     1321 cacaggtcaa tgaccacagc cagatacagc cagccttcat ctgtacgtaa gtacgtgatg
     1381 tctcccgccc acttctggtt cggaccactg gcgtaaaaat cctgctccag cagattttct
     1441 gacacaggca ggccatgtgc gcggtaactg accgtattgc tgggtaagat caatttgctc
     1501 actggtattt actacgtgca tcgaatgagt gatcaagagc cgtatgacgc gagagtgtca
     1561 cgtacggttc cgtaagcagc cggagagtga aactctctcc ggctgactcg actcgcaggt
     1621 tccgacagcg gtgacgaaca tgcagcggtg ttgttctcgc tgatcggcac atgccgtctg
     1681 aacaatgtgg agccagaaaa atggctgcgt tacgttattg aacatatcca ggactgaccg
     1741 gcaaaccggg tacgcgatct gttgccttgg aaagttgatc tgacttctca gtaaatatca
     1801 atatgattct gacgagccgc ttacctttga gcagagaaag gggagaggga gcccacatcc
     1861 atgagctagg aaaatacaaa aactggctat acaacaattc aatatattag gacttacttg
     1921 cgaggagaaa tatagcaccg ctcaggcaat tcctgggtat ttacttttct tagtttcttt
     1981 actcgggatg ttctgtcatc agaaaggcga ttctaaattt gtaatatagt cactcatatt
     2041 cccacgtaat ttctttagat caatatattg cacatttttc tcaaaaaact gatcggaaat
     2101 aagatttgca acattgcttg ttgtatcatt tttaacgctt ctattcatcc cttcacaaag
     2161 ttcgtccatt ttctttttga aataactgct tcctgataca agatgattta tggaactttg
     2221 tgcagcattg atgtctagag aaatgcgata atctttctca acctgtttaa atatctgttt
     2281 acggagatta tcgccgattt ttcggctgtg ttctgcacaa aacttatcaa tacattggtt
     2341 taccacccgg gatacgtaga cttttttttc atcatttttc caatctgtaa tattttcacc
     2401 gatttgttct gaaacagagc tcaaaatact agtatttatt ttggttgatg atatttttac
     2461 tatccttttc cccattgtac agcggataat ctcatccacc tttcctgaaa aatatctttt
     2521 gtgagataaa ctgattccaa aattattaaa agatgttcca agcattataa tcaccttgtc
     2581 atgtaaaata gtaacattgt cattatttta gctgattcaa acaattctgt aaatgctttg
     2641 agttctttac tcccttggct caatgatgga ccgtctggaa tcccagagac taaactctct
     2701 gagaaagaga gtatgaaaaa acttggggca gtccctaaat gaagggacca acagcggtaa
     2761 aataaccggc atcattcata atgatgccct gttagttgta gttatgaaga tttacgtcat
     2821 taaaattgct gttcgtagtg tcagtcctat agtctggcat cagctgagga tcgtagccga
     2881 tacatcactg gcttcacgac attcgtgttg aggctattca tgaaaactcc acgctgaaaa
     2941 cgccgttttg tataaacggt cacggtatgc ccggagccac tgttgctgat gaattcgata
     3001 aaaccttggc gtttcttgaa gctatcgtta atgcggataa tgaaacaacg attggtgaga
     3061 ttcgctcttt tgccgacgcc ctggacgctg tccggttcaa tcgcaacaaa atcaaccggc
     3121 agctgagcaa acccaacctt gcctctctgg cgctggagca tgaagtgatt tggctgggac
     3181 gtagccgctg acagcttcta taaaagtatg atcctaccca cgtaatatga acacaggctt
     3241 aagtgaggtt ctggttttca aattgttcag gactgagacc tccacaccaa ctgtgccgtc
     3301 gtcagcgatt gtaatcgcac ttgatataat taaacactgt atatttcatt tgtggagtct
     3361 ccagtctgag agggctttat gcattccgta ttgggcacta tgatgccaga aatctgcaag
     3421 gctccacacc ttcctgtatt tcccttcctc tatcacagtg cactttactt aatagggtgg
     3481 gatgtgttca ttacatttat tgtgtttttt atgattgttt ttgcgtccag tgtcgttcgt
     3541 catgggaaac tggttctatg ggtagtatcg ctcagtctag ttaactgttt gtttcttttt
     3601 agaaattaag cagatcacac aattcgggga gagatccttc taagtgcaat tctttgcagc
     3661 tatttagcac acttaaaaca actcttgtat tttgtcagaa ataatattaa acatgtcttg
     3721 ttcattattg ttaagtctgt ctgctatttc tgtaagtttt atcccactat gatggcatat
     3781 atttatatat gccatcatag cctcgatatt ccctttccct gcggccagaa ataatcctgg
     3841 taacccctca ttatctctta catccaatag tttaatcgtt tcttttggtg ataaatcaaa
     3901 agttttaata aaatctccat aagcttgaat cgcagcggaa tgaccttctt gaagagctat
     3961 gaacaaagca gattccccag cattatttcg tgctgcaaga acttctagtt tttcctcttt
     4021 gggtatttcg gctgccaaca gcgattcacc gtatttacgc gctgcatcgg catttccatg
     4081 ccaaatcaat gaaaacaaat tatggcggtt atactcctta ttattctttg tcggaatgga
     4141 tatgttctgt attttggcca attttctgaa agaaacacca ctatatcccc cccctgattt
     4201 ttcttcattg atggcgtggg atatagcggt atttgtcacg ttaatgatat tatttttcat
     4261 tcctttattt tcattagcag tattgctgcc tattattagg tgcatatttt tgttgtttgc
     4321 tggatgtaat ccatagttat ttattgacat ttttgttcat cctttttgct aaaaaaaaca
     4381 ataacctatt tttgtaatgt aatagatatt agtctattgc taatctaaaa aaaccattgc
     4441 cacattgaat ggctatattc accaataaat tctttcgatt tcgtatattg acatatcgta
     4501 gatatatctt atccctaaga ttcaataatc gagtccgttt accattctgt gcaagcgcct
     4561 tcactcagaa gtgaccgtca aggcgatcac caaacttgat aataaagcag ctcattgcca
     4621 tacgccagtc cttaaggggc gttgtccatt tctgtgatgc tgcctagatt taactgccag
     4681 ctccggctta tctacatcta aatatgtgag cctaataccg ctacactttg tccagactat
     4741 gtttttctcc gggaagtggg ctaaccgtct ccataaagtg acgaactttt ttcaacagtg
     4801 gccatattga acggcactga tgattacggg ttattgtctc atgaagagcc ggccacatca
     4861 ctgacagcag tgacttcagg ccttcagcac cagacagcct gaaccagtta atccatcgcc
     4921 cgacaaattt agtaatctag ggctggtttt tcttcagctg ttgcggttcg aaagtgccat
     4981 tacgatcacg cagagtacgg atttccagag gttcatctcc agtagtgata atcttcgtta
     5041 agcaaccatt gcgggaattg ctgccagatc taagctgatt tttctcgcac ccaagatgat
     5101 gagtcatatt agcattgagt acggtttcat tttcagcatc caatcaaact gactgagatc
     5161 ttcaggattt ttgagacttt tggctagttc attagctaaa gcccgcaact tattagatgt
     5221 caactgtttt tcattcataa attcaccttc atttgatatc aaattaaata gttcaacatc
     5281 agagaattat acaaacttat gtacaggctc ctatgatctt cgaatcctga agattatagt
     5341 gaaaataagt tttcacgttg aaatcccaca tagttagtat atgataaata atcatggatg
     5401 acctgattaa cctcttgggg tagtgctttt atagaagaat aaatattcta ctaatttaat
     5461 acatcaccta aaatagtatt gttttttatg gtcggttgaa ggtcgtggtg atgatgttag
     5521 tccttttgag aatattccag tgccagatct tacttataat aaaggggctt ggcagtttgg
     5581 ataaactatt gttcttattc cactaataca aactttttgt aaagtatatt aaatatactt
     5641 catttcatac tgaaatcccc ggaagtattc aatcaaacaa ttacaccata aaaggaagag
     5701 ggggccctaa ttaagttgtc aagcatgttt atgatacctg cagggtataa attttctggc
     5761 tgcaagcaga cacatataaa acgtctttat tgatcctacc cacataatat ggacacagtc
     5821 gtaagtgagg ctctggtttt caaattgttc cggactgaga ccaccacacc aacggtgccg
     5881 tcgccagcga ttgtaatcgc actcgatata actaaacact gttgcccgca ctatttcccg
     5941 atggataata gtgttctccg tggagacatt ccattttcag cgaatgaatt ttcatctgtt
     6001 gctgtatcca gtcatacagt gactgcatca gtggcgcggc tctggctttt cttgccgcca
     6061 gacgctgttc tgctgaacat ccccggactt ctgcctcgat ggcatacagt tcaccgatac
     6121 gctgcagggc ttccgtggtg atgtcggttg gcactcttgc atgcacatcg tggatttttc
     6181 tccggacatg agccatacat gcggcttccg ttattctgcc ggattcgtac aacgcccggt
     6241 aaccaccgta agcatcggcc tgaagcactc cgctgtaacc ggccagatga ttctgtggat
     6301 ggataccttt ccggtccggt gagtacgcga accagaccgc cggaggcatt tccgaaccgg
     6361 cgttacggtc atcacgaacg tagacccaca gccgggcagt ccttgtttta ccgctgcccg
     6421 gctcctggac cggaacgggg atatcatcgg catggacttt accgggcatc agcacatact
     6481 ggcgcaggat gtcatacagc ggttccagca gttcagcaac agcacctgtc cagcgcccca
     6541 gtgtggcacg gctcagctcc acgccctgac gacggtatat ttctgactgg cggtataacg
     6601 gcagatagtc tgcatatttt ccagcaacaa catgggccag aagccccgct ccggcataac
     6661 tgcgtgcaat gggttttgaa ggcaccggtg cctgcacgat atggtcacac cggcaacagg
     6721 ccagtttcgg acgttgtgtt tcgataacct taaaggcgct gctgataagc tccagctgct
     6781 ctgacacatc acatcccaga gaactgagtt cgccaccaca ggccgggcag cattcctctt
     6841 ccggacggat gacccgggtt tcacggggaa gtgaggcagg taacggttta cgggctgaag
     6901 actggcgcag gggagatggc agtaccgggt catattgctc acccagcgtt tccgccattt
     6961 cttcctgaag tgcgctgatt cgctcctgtg cttccagtat ctgccgttcg gtttttgcgc
     7021 gaagtttttc tgagctttta ccgaactgca tgcgctgcag tttcgcaacc agcgccttca
     7081 gccggttgat ttcggaagca taagccgcca cccgctgtga gagcaggcgg ttgtatttcg
     7141 ccatctggcg gatggtgtcc tgttgcgtct gcaacagtgc ccgcaggcgg gcattctcat
     7201 gagcaagtga ggtgtccata tcctcacttt acaacgggtt atatgcggat gccagcgcgt
     7261 tccgttcgtt tcgggtgttt ccagtcgatg ccctcaagaa gcatggataa ctgggccgga
     7321 gtaaggtgca ccttgccgtc acgggtgacc ggccagacga agcggccccg ctccaggcgt
     7381 ctggtaaaga ggcacagtcc gtcactgtca gcccacaaca cttttatctg gtcaccccgg
     7441 cgtccgcgga agatgaacag atgcccggag aacgggtcat ccttcaggac gttctgaact
     7501 tttgaggcca ggccgttaaa gccatttcgc atatcggtga tacctgcaac cagccagata
     7561 cgcgaacctg cagggaaaga tatcatcagt ggctgctccc ttttatttcg cggataagta
     7621 tctgtaataa cgccggcgtc agtttacctt taagcctgag agttccggcc ggcagaacca
     7681 gctcacagca cagactgtcg gacggtgtat ttatctgctc tggttcctgt gccggagccg
     7741 ggattttatt atccggctcc ggcgttaacg tcacgggaag cagtgcaggc atattttttc
     7801 cggaaggcag caggccacct ttccggtatt gatggcgcca gttgaagagc aggttatcgt
     7861 tgattccgtt ttcacgggcg atctgcgcca cacaggctcc gggctgcagt gactgctcca
     7921 ctaaagcgat tttaaattca taaggaaaat taggcc
//
LOCUS       DAJTRB010000153         7910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTRB010000153 DAJTRB010000000
VERSION     DAJTRB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.153"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(123..953)
                     /locus_tag="OO330_003882"
     CDS             complement(123..953)
                     /locus_tag="OO330_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5884563.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO330_003883"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO330_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5884564.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO330_003884"
     CDS             2379..2729
                     /locus_tag="OO330_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5884565.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO330_003885"
     CDS             2855..3415
                     /locus_tag="OO330_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5884566.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO330_003886"
     CDS             3418..6384
                     /locus_tag="OO330_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5884567.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO330_003887"
     CDS             6451..6828
                     /locus_tag="OO330_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5884568.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO330_003888"
     CDS             complement(7029..7688)
                     /locus_tag="OO330_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5884569.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1752 a         2242 c         2134 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaggg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DAJTRB010000154         7897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTRB010000154 DAJTRB010000000
VERSION     DAJTRB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.154"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..941)
                     /gene="hprS"
                     /locus_tag="OO330_003889"
     CDS             complement(<1..941)
                     /gene="hprS"
                     /locus_tag="OO330_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5884570.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="OO330_003890"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="OO330_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5884571.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="OO330_003891"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="OO330_003891"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5884572.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="OO330_003892"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="OO330_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000740079.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="OO330_003893"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="OO330_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5884573.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="OO330_003894"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="OO330_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5884574.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(5893..6162)
                     /locus_tag="OO330_003895"
     CDS             complement(5893..6162)
                     /locus_tag="OO330_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5884575.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            6219..6887
                     /locus_tag="OO330_003896"
     CDS             6219..6887
                     /locus_tag="OO330_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5884576.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            7203..>7897
                     /locus_tag="OO330_003897"
     CDS             7203..>7897
                     /locus_tag="OO330_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5884577.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2284 a         1771 c         1586 g         2256 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcaacggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctacgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattgct
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatctcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
     5461 tcgggagata agtcattgat aaaatggcgg agagaggggg atttgacccc ccggtagagt
     5521 tgcccctact ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa
     5581 tttttcttgg aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt
     5641 aaagaagaat attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg
     5701 tgatttgccc cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt
     5761 gttgaataat tccactgcag ttatataaaa tcggtaacgg ctggaaatca ttcaatactc
     5821 gcactatcga aagtttacca gccaaccgca gcacgtcctg catacgtcgt gtctgcggtt
     5881 tttctttttc gcttacattg tgtctggttc ttccggccac tcaatatcag gtgcagttga
     5941 tgtatcaaca cggttcagca acacccgata cttcttccag gcttccagca acgagttttc
     6001 ttcctccgtt gcgatctcca gatctacagc atcctgaagt ggcgcaatat gctcactgaa
     6061 tccctggatg tagaactgtg tggtgacggt cttccagcca ttcggctcct gctgtatcga
     6121 agcataccag gctatttcaa tatcgctatg ctgcggcagc atttaacccc ttgtaattca
     6181 tcgccataat tgatttaatt cacaaataaa actataatat ggtgaaatta atgaaaaaaa
     6241 acacagatga tggggctaaa atttacacac cacttaccct aaagctttat gactggtggg
     6301 ttttgggagt atcaaatcgg cttgcatggg gatgtcctac aaaggaacac cttcttccac
     6361 actttctgga acatttaggt aacaaccatc tggatattgg tgttggaact gggttttacc
     6421 ttactcacgt acctgagagt agtctgatat ctttaatgga tttgaacgaa gctagcctga
     6481 acgcggcatc tacaagggct ggggaatcaa aaattaaaca taaaattagc catgatgttt
     6541 ttgaacctta tcctgcggcg ttacatggtc aatttgattc catttccatg ttttaccttc
     6601 ttcactgcct gcctggaaat atatctacaa aaagctgtgt aatacgcaat gcggcgcagg
     6661 ccttaactga tgatggaact ctatacggag ccacaattct tggcgatgga gttgtgcaca
     6721 atagcttcgg tcaaaaactg atgcgcattt acaatcagaa aggcatcttt tcaaacacaa
     6781 aagattccga agaaggctta acacatatac tctcagagca tttcgagaat gttaaaacca
     6841 aggttcaagg tactgtagta atgttttccg cttcagggaa aaaatagcat ccaaccgcag
     6901 cacgttcttg cttaagacgt gctacggttt cctttacgat ttggggttgc gactttacct
     6961 ctattgataa tgcattccgg cagaacgttc aaatatgaac gtacgatatt taactaaccg
     7021 aaaaacaaaa tatagcacag gcgagacatt taccatctaa gaaaatagta tcgttttttt
     7081 acagttaaat caacatcact tccttaaaat gaaaacaata aatttcaatc ctgaaacatt
     7141 ttatttcagc ttattattcc ccagtatcac attttatcag gatatccttc tgcgaggtta
     7201 ttatgcttcc tgtaaataat ccccccctat ccactggaaa cgtctctttt tacagaacta
     7261 catcaatcga caatgttcac aataattatc tctccgaatg ggttgaatgg actaaaaaca
     7321 gcatttccgg agaaaacagg gaaactgctt ttacccggct ccaattatgt ctggagaaca
     7381 gtgaaacatc gttggactta tcttgtttag gtctcagatc tctaccacga ttgcctgaca
     7441 atcttgatga aattaatgta agcaataacc aactatcaat gctccccgag ctaccaaggg
     7501 cattgaaaga gctgaatgca agcagtaatc aattatctgc acttcctgaa ttaccagtgt
     7561 cgctggaata tataaatgtg agtgataacc atttgttcgc acttcctgaa ttacctgcgt
     7621 cactagaata tattaatgta agtgacaatc acctgtctgt acttccgagg ttaccaatgt
     7681 cattggaatt acttgatgca gccagaaatg ctttggaagt aataccagat tttccagaaa
     7741 gagatgatca tattataaga atattctggc ttaatcagaa ccggatcacg gcaattccgg
     7801 aaagcatact tggcctcagt tctgatagcg ttgtcaatct tagagaaaat caactatctc
     7861 ccagaataat gcaaactttg ttacaacaaa ccgccca
//
LOCUS       DAJTRB010000155         7841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTRB010000155 DAJTRB010000000
VERSION     DAJTRB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.155"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(120..833)
                     /gene="msyB"
                     /locus_tag="OO330_003898"
     CDS             complement(120..833)
                     /gene="msyB"
                     /locus_tag="OO330_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5884578.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(859..1263)
                     /locus_tag="OO330_003899"
     CDS             complement(859..1263)
                     /locus_tag="OO330_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5884579.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            1640..3556
                     /gene="dnaK"
                     /locus_tag="OO330_003900"
     CDS             1640..3556
                     /gene="dnaK"
                     /locus_tag="OO330_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5884580.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3645..4775
                     /gene="dnaJ"
                     /locus_tag="OO330_003901"
     CDS             3645..4775
                     /gene="dnaJ"
                     /locus_tag="OO330_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5884581.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4879..5031)
                     /gene="mokC"
                     /locus_tag="OO330_003902"
     CDS             complement(4879..5031)
                     /gene="mokC"
                     /locus_tag="OO330_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5884582.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            5617..6783
                     /gene="nhaA"
                     /locus_tag="OO330_003903"
     CDS             5617..6783
                     /gene="nhaA"
                     /locus_tag="OO330_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5884583.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            6849..7748
                     /gene="nhaR"
                     /locus_tag="OO330_003904"
     CDS             6849..7748
                     /gene="nhaR"
                     /locus_tag="OO330_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5884584.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVTDA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
BASE COUNT         1953 a         1862 c         2033 g         1993 t
ORIGIN      
        1 ggtgaaataa tttccctgaa taactgtagt gttttcaggg cgcggcataa taatcagcca
       61 gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa ttttccccgg tttccggtat
      121 cagacctgag tggcgctaac catccggcgc aggcaggcga tttgcagtac ggctggaatc
      181 gtcacgcgat aggtgctgcc gctgaccgct ttaaccccat ttaatgctgc gcccacaggg
      241 cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta cgctcattgc tgcgtgggtg
      301 cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca caccttttgc caataatttg
      361 tctttcatca gcagcggcag cagctcttcc agctcattca ccctggcatc gaccgcgtgc
      421 agaaactcct gcttatgttc ctcgtccatt ttcttccagg tattacgcag aaattgttcc
      481 agtaactgtt gctcaatttc aaacgtagac atctctttgt cggctttcag cttcaatcgc
      541 tttgaaacat cgagcaaaat ggcccgatac aatttaccgt gtccgcgcag tttgttggcg
      601 atactatcgc caccaaaatg ctgtaattct ccggcaatca gctgccagtt gcggcgatgt
      661 tgctcgggat gcccttccat cgatttaaac agttcgttgc gcatcagtat gctggagagg
      721 cgagttttgc ctttttcatt atgggtgagc aatcgggcga aatttgccaa ctgttcctca
      781 ctacaatgct gaagaaaatc cagatctgaa tcattcaggt aattaacatt cattttttgt
      841 ggcttctata ttctggcgtt agtcgtcgcc gataattttc agcgtggcca tatccgatga
      901 gttcaccgta tgaccggaaa aggtgatttt tgagacgcag cgtttattgt cgttatcgct
      961 gttaatgttg atccagtcag tggtttgccc ttcttttatt tctgaaggaa tattcaggct
     1021 ctgactggcg ctacgggcgg ctttgaaata aaccgatgca ccacttaact gtaaatcgcc
     1081 atggtcggca gagagttgta tgcgtttcac aatgcgacaa acaggaagtt tcagcgccag
     1141 atcgttggtt tcgttacgcg gcattgcaat gacgccgagg agtttatggt cgtttgcctg
     1201 cgccgtgcag cacagcatca ggctaatcgc caggctggcg gaaatcgtaa aaacggattt
     1261 cataaggatt ctcttagtgg gaagaggtag ggggatgaat acccactagt ttactgctga
     1321 taaagagaag attcaggcac gtaatctttt ctttttatta caattttttg atgaacgcct
     1381 tggctgcgat tcattcttta tatgaataaa attgctgtca attttacgtc ttgtcctgcc
     1441 atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt
     1501 gggttacgac cccatttagt agtcaaccgc agtgagtgag tctgcaaaaa aatgaaattg
     1561 ggcagttgaa accagacgtt tcgcccctat tacagactca caaccacatg atgaccgaat
     1621 atatagtgga gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc
     1681 ttgtgtagcg attatggatg gcaccattcc tcgcgtgctg gagaacgccg aaggcgatcg
     1741 caccacgcct tctatcattg cctataccca ggatggtgaa actctggttg gtcagccggc
     1801 taaacgtcag gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg
     1861 ccgccgcttc caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat
     1921 tgctgctgat aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca
     1981 gatttctgct gaagtgctga aaaaaatgaa gaaaaccgct gaagattacc tgggtgaacc
     2041 ggtaactgaa gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac
     2101 caaagacgca ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc
     2161 agctgcgctg gcttacggtc tggacaaagg cactggcaac cgtactatcg cggtttatga
     2221 cctggggggt ggtactttcg atatttctat tatcgaaatc gacgaagttg acggcgaaaa
     2281 aaccttcgaa gttctggcaa ccaacggtga tacccacctg ggtggtgaag acttcgacag
     2341 ccgtctgatc aactatctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa
     2401 cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc
     2461 ttccgctcag cagaccgacg ttaacctgcc atacatcact gcagacgcga ccggtccgaa
     2521 acacatgaac atcaaagtga ctcgtgcgaa actggaaagc ctggttgaag atctggtaaa
     2581 ccgttccatt gagccgctga aagttgcact gcaggatgct ggcctgtccg tatctgatat
     2641 cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc
     2701 tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg
     2761 tgctgctgtt cagggtggtg ttctgactgg cgacgtaaaa gacgtactgc tgctggacgt
     2821 taccccgctg tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa
     2881 aaacaccact atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc
     2941 tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct
     3001 gggtcagttc aacctagatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt
     3061 taccttcgat atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg
     3121 taaagagcag aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa
     3181 aatggtacgc gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca
     3241 gactcgcaac cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg
     3301 cgacaaactg ccggctgacg acaaaactgc tatcgagtct gcgctgactg cactggaaac
     3361 tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa atgcaggaac tggcacaggt
     3421 ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc
     3481 tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt
     3541 caaagacaaa aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa
     3601 tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt
     3661 attacgagat tttaggcgtt tccaaaacag cggaagagcg tgaaatcaga aaggcctaca
     3721 aacgcctggc catgaaatac cacccggacc gtaaccaggg tgacaaagag gccgaggcga
     3781 aatttaaaga gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat
     3841 acgatcagta tggtcatgct gcgtttgagc aaggaggcat gggcggcggt ggttttggcg
     3901 gcggcgcaga cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac
     3961 gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg
     4021 aagaagctgt acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg
     4081 tttgccacgg tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg
     4141 gttctggtca ggtgcagatg cgccagggat tcttcgctgt acagcagacc tgtccacact
     4201 gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg
     4261 ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca
     4321 tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc accggcaggc gatctgtacg
     4381 ttcaggttca ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctgtattgcg
     4441 aagtcccgat caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg
     4501 atggtcgcgt caaactgaaa gtgcctggcg aaacccagac cggtaagcta ttccgtatgc
     4561 gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg tgatttgctg tgccgcgttg
     4621 tcgtcgaaac accggtaggc ctgaacgaaa agcagaaaca gctgctgcaa gagctgcaag
     4681 aaagcttcgg tggcccaacc ggcgagcaca acagcccgcg ctcaaagagc ttctttgatg
     4741 gtgtgaagaa gttttttgac gacctgaccc gctaacctcc ccaaaagcct gcccgtgggc
     4801 aggcctgggt aaaaataggg tgcgttgaag atatgcgagc acctgtaaag tggcggggat
     4861 cactccccgc cgttgctctt actcggattc gtaagccgtg aaaacagcaa cctccgtctg
     4921 gccagttcgg atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacggc
     4981 ggtgatacag atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt
     5041 ctccttgacc ttacggtcag taagaggcac tctacatgtg ttcagcatat aggaggcctc
     5101 gggttgatgg taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga
     5161 gacagacagc ctcaagcacc cgccgctatt atatcgctct ctttaaccca ttttgtttta
     5221 tcgattctaa tcctgaagac gcctcgcatt tttgtggcgt aattttttaa tgatttaatt
     5281 atttaacttt aatttatctc ttcatcgcaa ttattgacga caagctggat tatttttgaa
     5341 atattggcct aacaagcatc gccgactgac aacaaattaa ttattacttt tcctaattaa
     5401 tccctcagga atcctcacct taagctatga ttatctgggc ttagggtcac tcgtgagcgc
     5461 ttacagccgt caaaaacgca tctcaccgct gatggcgcaa attcttcaat ggctcgtaaa
     5521 aaacgaatta ttcctacact ataatctgat tttaacgatg attcgtgcgg ggtaaaatag
     5581 taaaaacgat ctattcacct gaaagagaaa taaaaagtga aacatctgca tcgattcttt
     5641 agcagtgatg cctcgggagg cattattctt atcattgccg ctatcctggc gatgattatg
     5701 gccaacagcg gcgcaaccag tggatggtat cacgactttc tggagacgcc ggttcagctc
     5761 cgggttggtt cactcgaaat caacaaaaac atgctgttat ggataaatga cgcgctgatg
     5821 gcggtatttt tcctgttagt cggtctggaa gttaaacgtg aactgatgca aggatcgcta
     5881 gccagcttac gccaggccgc atttccagtt atcgccgcta ttggtgggat gattgtgccg
     5941 gcattactct atctggcttt taactatgcc gatccgatta cccgcgaagg gtgggcgatc
     6001 ccggcggcta ctgacattgc ttttgcactt ggtgtactgg cgctgttggg aagtcgtgtt
     6061 ccgttagtgc tgaagatctt tttgatggct ctggctatta tcgacgatct tggggccatc
     6121 attatcatcg cattgttcta cactaatgac ttatcgatgg cctctcttgg cgtcgcggct
     6181 gtagcaattg cggtactcgc ggtattgaat ctgtgtggtg tacgccgcac gggcgtctat
     6241 attcttgttg gcgtggtgtt gtggactgcg gtgttgaaat cgggggttca cgcaactctg
     6301 gcgggggtaa ttgtcggctt ctttattcct ttgaaagaga agcatgggcg ttctccagcg
     6361 aagcgactgg agcatgtgtt gcacccgtgg gtggcgtatc tgattttgcc gctgtttgca
     6421 tttgctaatg ctggcgtttc actgcaaggc gtcacgctgg atggcttgac ctccattctg
     6481 ccattgggga tcatcgctgg cttgctgatt ggcaaaccgc tggggattag tctgttctgc
     6541 tggttggcgc tgcgtttgaa actggcgcat ctgcctgagg gaacgactta tcagcaaatt
     6601 atggtggtgg ggatcctgtg cggtatcggt tttactatgt ctatctttat tgccagcctg
     6661 gcctttggta gcgtagatcc agaactgatt aactgggcga aactcggtat cctggtcggt
     6721 tctatctctt cggcggtaat tggatacagc tggttacgcg ttcgtttgcg tccatcagtt
     6781 tgacaggacg gtttaccggg gagccataaa cggctccctt ttcattgtta tcagggagag
     6841 aaatgagcat gtctcatatc aattacaacc acttgtatta cttctggcat gtctataaag
     6901 aaggttccgt ggttggcgca gcggaggcgc tttatttaac tccacaaacc attaccggac
     6961 agattcgagc gctggaagag cgcctgcaag gcaaattatt taaacgcaag ggacgtggtc
     7021 tcgaacccag cgagctggga gaactggtct atcgctatgc cgataaaatg ttcaccttaa
     7081 gccaggaaat gctggatatt gtgaactatc gcaaagaatc caatttattg tttgacgttg
     7141 gcgtgactga tgcactttcc aaacgcctgg tcagtagcgt acttaacgcc gcagtggtag
     7201 aaggcgagcc cattcatctt cgctgcttcg aatccaccca cgaaatgctg ctggagcaat
     7261 taagtcagca taaactggat atgatcattt ctgactgtcc gatagactct acgcagcagg
     7321 aaggcctgtt ctccgtgaga attggcgaat gtggcgtgag tttctggtgt acaaatccac
     7381 caccagaaaa accgttcccg gcttgtctgg aagaacggcg acttttgatt cctgggcgac
     7441 gttcaatgtt agggcgcaaa ttgcttaact ggtttaactc ccagggatta aacgtagaaa
     7501 tcctcggcga gtttgatgat gccgctttga tgaaagcttt tggtgcgatg cacaatgcaa
     7561 tcttcgttgc cccaacgctt tatgcatatg acttttatgc cgataaaact gtcgtagaaa
     7621 ttggtcgcgt cgagaatgtg atggaagagt accatgctat ttttgctgag cggatgattc
     7681 agcacccggc ggtacagcga atctgcaata cggattattc tgcgcttttt agtccagcgg
     7741 tgcgttaatc ggcagctccc ccaaagttaa ggtgggggag atagattagt tgtacattac
     7801 cacgattttg actcggctca ttatttgccc gcttgagaca t
//
LOCUS       DAJTRB010000156         7774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTRB010000156 DAJTRB010000000
VERSION     DAJTRB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.156"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..120
                     /locus_tag="OO330_003905"
     CDS             <1..120
                     /locus_tag="OO330_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5884585.1"
                     /translation="TWSYDALGAGAERVLSLSGMGTIENAGDDDHEALTPEKS"
     gene            129..458
                     /locus_tag="OO330_003906"
     CDS             129..458
                     /locus_tag="OO330_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5884586.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            430..3471
                     /locus_tag="OO330_003907"
     CDS             430..3471
                     /locus_tag="OO330_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5884587.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            3471..3800
                     /locus_tag="OO330_003908"
     CDS             3471..3800
                     /locus_tag="OO330_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5884588.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            3800..4498
                     /locus_tag="OO330_003909"
     CDS             3800..4498
                     /locus_tag="OO330_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5884589.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            4503..5246
                     /locus_tag="OO330_003910"
     CDS             4503..5246
                     /locus_tag="OO330_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5884590.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            5183..5785
                     /locus_tag="OO330_003911"
     CDS             5183..5785
                     /locus_tag="OO330_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5884591.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            5846..>7774
                     /locus_tag="OO330_003912"
     CDS             5846..>7774
                     /locus_tag="OO330_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5884592.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDT"
BASE COUNT         1788 a         1859 c         2573 g         1554 t
ORIGIN      
        1 acatggtcgt atgatgcgct gggcgcgggg gcggagaggg ttctgtcgct gagcggtatg
       61 gggaccattg agaatgccgg ggatgatgat catgaggcgc tgacgccgga aaagtcctga
      121 cgcgggaaat gcagtttgtc atgcggcttg cccgggagtt ccggcgggca gactggcggc
      181 ggatgctgtc ggatatgtcg gccactgagc ttggtgagtg gggcgattat ttccggatgc
      241 agagcttcag tgatgtgtgg atggatgcgc agtttgcctc gctgaaggca ttgatcgtga
      301 gaatggtgtc cggcagcagc gatgctgcgg tggctgattt cagcctttta ccggaagaga
      361 acgggatacc ggagcgaacg gacgaagaac tgatgcatct tggggaaggt atttccggag
      421 gtgtgcgtta tggaccagat agccaacctg gtcattgatt tggggattga tgcggcagag
      481 tttaaaaatg aaattccccg tatcaaaaac cttctgaatg gtgcagccag cgatgcagaa
      541 cggtcttctg cccgtatgca gcgttttatg gagcgtcaga ctcaggcggc ccggcagaca
      601 atgcaggcgg cttcttcggc tgcaacagca gcatcagccc atgcgcagac ggtggagaag
      661 aatgcacggg ctcatgaacg catggcccgt gaggtggaac aaacccgtct gcgcgtggat
      721 gccctgaatc agaaaatgcg cgaggaacag gcgcaggcca gggcactggc ggaggcgcag
      781 gataaagcgg ctgccgcctt ttatcgccag attgacagtg tgaaacaggc cggtgcgggg
      841 cttcaggaat tacagcgtat tcagcagcag atccgacagg ccagaaacag tggcggggtt
      901 ggtcagcagg attatctggc gctgatttcg gagatcacgg cgaaaacccg tgccctgacg
      961 caggcagagg aacaggccac ccggcagaaa gcggcgttta tccgccagct taaagagcag
     1021 gcaacccgcc agaatctgtc gtcttctgag ttgcttcgcg ccagggcggc tcagctgggg
     1081 gtaagcagtg ctgcagaagt gtatatccgc aaaatggagc gggcaggaaa agccacacat
     1141 tcgctggggc tgaaaagtgc ggcagcccgg cgggagctgg gggtgttaat cagtcagatg
     1201 gcgcgcggca attttggtgc gctgagggga tccgggataa cgctggctaa ccgtgccgga
     1261 tggacaggcg cactgatgtc gccgaaaggc atgatgactg gcggcgttat tggcggactt
     1321 gtcgcggcgg tcctgggtct gggtaaagcc tggcatgacg gccggaagga gggcgagaaa
     1381 tttaaccgtc agctggcgct gacgggacat tatgccggtg tcactgtcgg gcagttgtgg
     1441 aaactcagcc gggccatatc cgggaatggt atcacgcaac atgcggcagc cggtgcgctg
     1501 gcacaggtgg tgggcagtgg ggcatttcat ggaaacgata ttggtatggt ggcgaaagct
     1561 gccgcacaga tggagcgatc ggttggccag tcggtcagcg ataccataaa tcagtttaag
     1621 cggctgaagg atgatcctgt aaatgccgcg aaggcgctgg acaatgcgct gcattttttg
     1681 aatgccactc agcttgagca gatacgcgtc cttggggagc aggggcggtc cagtgatgcg
     1741 gcccggatcg ccatgtctgc gctggcagag gaaaccggta aacgcacatc tgatattgat
     1801 aataatctca atgcgctggg tagtacgctg caaaccttgt ctgactggtg gaagcagttc
     1861 tgggatgcgg ccatgaatat tggtcgtgaa gactcgctgg atgcgcagat tgatgcgtta
     1921 caggaaaaaa ttcagcgcgc gaaaaaatat ccgtggacaa acgcctccac acaggtggag
     1981 tacgatcagc agcgtcttaa cgatcttcag gagaaaaaac gccggaagga tttgcaggat
     2041 gcaaaagcgc aggcagaacg gaattaccag gagcaacaga aacgccggaa tgctgaaaat
     2101 gccgcgctga accggatgaa tgaaacggaa gctgcacgac atcagcggga aattgcgcgt
     2161 attaatgcca tgcagtacgc cgaccaggct gtcagggatg cggcgataca gcgtgaaaat
     2221 gaacgttacg aaaaagccat taagaaaaat acacgggcaa cccgtaatga tgaggccacc
     2281 cggttattgc tgcagtacag tcagcagcag gcacaggtgg aagggcagat tgctgctgcc
     2341 agacagtcag caggcattgc cactgaaagg atgactgaag cgcataaaca gcttctggcc
     2401 ctgcagcagc gcatcagcga cctggacggg aaaaagctga cggcagatga aaagagtgtg
     2461 ctggcccgta agaatgagct gattcaggcg ctgacgctgc tggatgtgaa acagcaggag
     2521 ctgcagaaac agacagcgct taacgacctg agaaaaaaaa cggttcagct gaccagccag
     2581 ctggcagaca aagaacgtgc actgcgtgag cagcacaatc tggatattgc cactgcaggt
     2641 atgggggata agcagcggca gcgctaccag gcacagttgc gcatccggca ggaataccgg
     2701 caacagttgc aacagcttga gaatgacagt cgccagaaag gcacttacgg gacggaggac
     2761 taccggaggg ctgaggaggt gctgaagggg agcctgaagc gacaactgaa tgaaaacaaa
     2821 cgctactggc aggaactgga agtggcgcag ggcgactgga aaaacggtgc catgcgggcg
     2881 tttcagaatt ttacggcgga tgcggataat gcggcgggaa cggcagaaca gatgtttaca
     2941 gtggcattca gcagtgccgg taatgcactg gcgacattct gtaccaccgg taagctgaat
     3001 tttaaatcct tcacctcttc cctgttgtca gatatggcca gaattatggc acagatggcc
     3061 atgatgcagg cggtaaaggg cgtcggttct ttattcggct tcacgactaa tgctgatggc
     3121 ggtgtttacc agtctgctga tttgagtcgc tacagtggca cggtggttaa ccgtccgacg
     3181 ttttttgctt ttgcaaaagg cgcgggtgtg atgggggaag cgggacctga agccattctg
     3241 ccactgcgtc gcggtgctga tggtaagctg ggggttgtgg cggatactgg tggttcaggc
     3301 atggtgatgt ttgccccgca gtacaacatt gagatcaata acgacggcac gaacgggcag
     3361 ataggtccgg ctgccctgaa ggtggtttat gacctcggga aaaaagcggc cgcggacttt
     3421 atgcaacagc aggcccgtga tggtggccgg ttaagtggag cataccggta atggagacgt
     3481 ttcactggaa agtgcgcccg gatatgaatg tggtatcaga gccgaaagtg gtgacagtga
     3541 agctgggcga tggttatgaa cagcgtcgtg cggcgggact gaataaccag ttgtcgactt
     3601 acagcgtgac gatacgtgtt cgtaaaggtg aacacccatc tttaaaagcc tttctggaac
     3661 ggcacggtgg cgtccgcgca tttcagtgga cgccacctta tgactggaaa ctgatcaggg
     3721 tggtttgtcg taaatggtcg gcaagcgtgg gggcgttatg ggtgactgta acggccgatt
     3781 ttgaacaggt ggtgaactga tgcaggatat ccggcaggaa acactgaatg aatgcacccg
     3841 tgcggagcag tcagcccggg tggagctctg ggaaatcgat ctgacagagg tcggtggtga
     3901 gcgttatttt ttctgtaatg agcagaacga aaaaggtgag ccggtcacct ggcaggggcg
     3961 gcagtatcag gcatacccca ttcaggggtc ggggtttgaa ctgaacggca ggggctgtgc
     4021 tgcccgtccg acactgacgg tctctaacct gcacggcatg gtcaccggga tggcggaaga
     4081 tctgcagagt ctggtcggcg gaacggtggt ccggcgtaag gtttacgccc gttttctgga
     4141 tgcggtgaac ttcgtcaacg gaaacagcga cgccgatccg gaacaggagg tgatcagccg
     4201 ctggcgcatc gagcagtgca gcgaactgag cgcggtcagt gcctcttttg tgctggccac
     4261 accaacggag acggatggcg cggttttccc ggggcgtatc atgctggcga atacctgtat
     4321 gtggacttac cgttctgatg agtgtggtta cacgggcagg gcagtggctg acgagttcga
     4381 caaaccaaca acggatatcc ggaaggacaa atgcagcaag tgtatgcgcg ggtgtgagtt
     4441 gcgcaacaat accggtaatt tcggcggttt cctttccatc aataaacttt ctcagtaaat
     4501 ccatgacaca gacagaatca gcgattctgg cgcacgcccg gcgatgtgcg ccagcggagt
     4561 cgtgtggctt cgtggtgaga acgccggagg gagacaggta tcttcccagc gagaatatct
     4621 ccggtgagcc ggaggaacgg ttccggatgg ctccggagga ctggctgcgg gcacaaatgc
     4681 agggtgagat tgtggcactg gtccacagtc atcccggtgg tctgccctgg ctgagtgagg
     4741 ctgaccggcg gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc cggggggcga
     4801 ttcacaagtt ccgctgtgtg ccgcatctca ccgggcggcg ctttgagcac ggggtgacgg
     4861 actgttacac gctgttccgg gatgcttatc atctggcggg gattgagatg ccggattttc
     4921 atcgcgggga tgactggtgg cgtcacggtc agaatctcta tctggataat ctggaggcca
     4981 cagggctgta tcaggtgccg ttgtcatcag cacaaccggg cgatgtgctg ctgtgctgtt
     5041 ttggttcatc ggtgccgaat catgccgcca tttactgcgg cgatggtgag ctgctgcacc
     5101 atattcctga acaactgagt aaacgagaga ggtataccga caaatggcag cgacgcacac
     5161 actccctctg gcgtcaccgg gaatggcacg catctgcctt tacggggatt tgcaacgatt
     5221 tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa gccatccgcg cactggccac
     5281 acagctcccg gcgtttcgtc agaaactgag tgacggctgg tatcaggtgc gcattgccgg
     5341 gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat gagccgctgg caaatggtgc
     5401 agtgatccac atagtaccgc gtctggtggg agctaaaagt ggcggtgtgt ttcaggcggt
     5461 gctgggggca gctgttatgg cggttgctat atggatgccg ggggtaggaa ttatggcgag
     5521 taatctgctg ttttctctcg gtgccagtat gacgcttggc ggtgttgcac agatgctggc
     5581 accgaaagcc agaactcccc gtacacagac aacggataac ggcaaacaga acacctgttt
     5641 ctcctcactg gataacatgg ttgcccaggg caatgttctg cctgttctgt acggtgaaat
     5701 gcgcgtgggg tcgcgggtgg tatctcagga gatcagcacg gcagacgaag gggatggtgg
     5761 tcaggttgtg gtgattggtc gctgatgcaa aatgtttcat gtgaaaccgc ctgcgggcgg
     5821 ttttgtcgtt tatggagcgt gaggaatggg taaaggcagc agtaaggggc ataccccgcg
     5881 cgaagcgaag gacaacctga aatccacgca gttgctgagt gtgattgatg ccatcagcga
     5941 agggccggtt gacggtccgg tggatggatt aaaaagcgtg ctgctgaacg gtacgccggt
     6001 cctggatagc gaggggaaga caaacttttc cggtgttacg gtggtgttcc gcgccggcga
     6061 gcaggagcag acaccgccgg aggggtttga atcttccggc tcagagactg tgctgggtac
     6121 ggaagtgaaa tacgacacgc cgatcacccg gaccatcacg tcggcaaaca ttgatcgtct
     6181 gcgcctgacc ttcggtgtgc aggcactggt ggaaaccacc tcaaaggggg accggaatcc
     6241 gtcggaagtc cgcctgctgg ttcagataca gcgtaacggt ggctgggtga cggaaaaaga
     6301 catcaccatt aagggcaaaa ccacttcgca gtatctggcc tcggtggtgg tgggtaacct
     6361 gccgccgcgc ccgtttaata tccggatgcg caggatgaca ccggacagca ccacagacca
     6421 gctgcagaac aaaacgctct ggtcgtcgta caccgaaatc atcgatgtga aacagtgcta
     6481 cccgaacacg gcgctggtcg gcgtgcaggt ggattcagag cagttcggta accagcaggt
     6541 gagtcgcaat tatcatcttc gcgggcgcat tctgcaggtg ccgtcgaact ataacccgca
     6601 gacgcggcaa tacagcggta tctgggacgg aacgcttaag ccagcataca gcaacaacat
     6661 ggcctggtgt ctgtgggaca tgctcactca tccgcgctac ggcatgggga aacgtcttgg
     6721 tgcggcagat gtggacaaat gggcgctgta tgtcatcggc cagtactgcg accagtcagt
     6781 gccggacgga tttggcggca cggagccgcg catcacctgt aacgcttacc tgaccacaca
     6841 gcgtaaggcg tgggatgttc tcagtgattt ctgctcggcg atgcgctgta tgccggtatg
     6901 gaacgggcag acgctgacgt tcgtgcagga ccgaccgtcg gataaagtgt ggacctataa
     6961 ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc cgctacagct tcagcgccct
     7021 gaaggaccgc cataatgtcg ttgaggtgaa ctggattgat ccggacaacg gtcatgagac
     7081 ggcgacagag cttgtggagg acacgcaggc cattgtccgt tacggtcgta acgtcacgaa
     7141 gatggatgcc tttggctgta ccagccgggg acaggcgcac cgcgccgggc tgtggctgat
     7201 taaaacggaa ctgctggaaa cgcagaccgt ggacttcagc gtcggcgcag aagggcttcg
     7261 ccatgtaccg ggtgatgtca ttgaaatctg cgatgatgat tatgccggta tcagcaccgg
     7321 tggtcgcgta ctggcggtga acagccagac ccggacgctg acgctcgacc gtgaaatcac
     7381 gctgccgtcc tccggtacca cgctgataag cctggttgac ggaaatggca atccggtcag
     7441 cgtggaggtt cagtccgtca ccgacggcgt gaaggtgaaa gtgagccggg ttcctgacgg
     7501 cgttgcagaa tacagcgtgt gggggctgaa gctgccgacg ctgcgccagc gcctgttccg
     7561 ctgcgtgagt atccgtgaga acgacgacgg cacgtatgcc atcaccgccg tgcagcatgt
     7621 gccggaaaaa gaggccatcg tggataacgg ggcacacttt gacggtgacc ggcgcggcac
     7681 ggtgaatggt gtcacgccgc cagcggtgca gcatctgacc gccgaagtca ccgcagacag
     7741 cggggaatat caggtgctgg cgcgatggga cacg
//
LOCUS       DAJTRB010000157         7747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTRB010000157 DAJTRB010000000
VERSION     DAJTRB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.157"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="OO330_003913"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="OO330_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5884593.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMLEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="OO330_003914"
     CDS             1104..2564
                     /locus_tag="OO330_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5884594.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="OO330_003915"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="OO330_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5884595.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="ydfH"
                     /locus_tag="OO330_003916"
     CDS             complement(2981..3667)
                     /gene="ydfH"
                     /locus_tag="OO330_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5884596.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDADAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="OO330_003917"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="OO330_003917"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5884597.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQRHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="OO330_003918"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="OO330_003918"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5884598.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="OO330_003919"
     CDS             complement(6729..7247)
                     /locus_tag="OO330_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5884599.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVTAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7747
                     /gene="ydeI"
                     /locus_tag="OO330_003920"
     CDS             7523..>7747
                     /gene="ydeI"
                     /locus_tag="OO330_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5884600.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1942 a         1809 c         1958 g         2038 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc tagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg gcatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cctttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgtca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caaagga
//
LOCUS       DAJTRB010000158         7719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTRB010000158 DAJTRB010000000
VERSION     DAJTRB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.158"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..830)
                     /locus_tag="OO330_003921"
     CDS             complement(<1..830)
                     /locus_tag="OO330_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5884601.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="OO330_003922"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="OO330_003922"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5884602.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="OO330_003923"
     CDS             complement(2479..2706)
                     /locus_tag="OO330_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5884603.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="OO330_003924"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="OO330_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5884604.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="OO330_003925"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="OO330_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5884605.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="OO330_003926"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="OO330_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5884606.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="OO330_003927"
     CDS             complement(5528..6511)
                     /locus_tag="OO330_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5884607.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="OO330_003928"
     CDS             complement(6557..7381)
                     /locus_tag="OO330_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5884608.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="OO330_003929"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="OO330_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5884609.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1811 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcact
//
LOCUS       DAJTRB010000159         7536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTRB010000159 DAJTRB010000000
VERSION     DAJTRB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.159"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO330_003930"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO330_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO330_003931"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO330_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5884610.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO330_003932"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO330_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO330_003933"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO330_003933"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5884611.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANISFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO330_003934"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO330_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5884612.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO330_003935"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO330_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5884613.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO330_003936"
     CDS             complement(6510..7202)
                     /locus_tag="OO330_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5884614.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1919 a         1825 c         1984 g         1808 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatggacgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcaa tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tctcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc gggggtctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcg
//
LOCUS       DAJTRB010000160         7188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTRB010000160 DAJTRB010000000
VERSION     DAJTRB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.160"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO330_003937"
                     /pseudo
     CDS             complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO330_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            764..916
                     /locus_tag="OO330_003938"
                     /pseudo
     CDS             764..916
                     /locus_tag="OO330_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2297..3244
                     /gene="icsP"
                     /locus_tag="OO330_003939"
     CDS             2297..3244
                     /gene="icsP"
                     /locus_tag="OO330_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5884615.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3806..4178)
                     /locus_tag="OO330_003940"
                     /pseudo
     CDS             complement(3806..4178)
                     /locus_tag="OO330_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4422..4541
                     /locus_tag="OO330_003941"
                     /pseudo
     CDS             4422..4541
                     /locus_tag="OO330_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4791..5657
                     /gene="ospB"
                     /locus_tag="OO330_003942"
     CDS             4791..5657
                     /gene="ospB"
                     /locus_tag="OO330_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5884616.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5986..6726
                     /gene="phoN2"
                     /locus_tag="OO330_003943"
     CDS             5986..6726
                     /gene="phoN2"
                     /locus_tag="OO330_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5884617.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7086..>7188)
                     /locus_tag="OO330_003944"
     CDS             complement(7086..>7188)
                     /locus_tag="OO330_003944"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5884618.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2250 a         1354 c         1388 g         2196 t
ORIGIN      
        1 acaattaata tacattaagt aacaggcatt cgagccttga tttgagaaag cctgtcattc
       61 atttgtttta tgtagctatc tatttcctct gatgcataac taaaaagttc tcccatttca
      121 cgagcaataa taatctcaga tttaatagac tttaatctct ggcgactcat ctcttaacac
      181 ttgcataatc tctcttttta tatcatatag ttctataaac cgatttgccc gtagttctcc
      241 ttggggagaa tcatttctga atacaacaat tatatacaaa aaatcactat tttctttaga
      301 gaagatctta caactaatct ctgggagctc acataatgca tgtaagtcag gatggtacaa
      361 actatccaca gaaaattgcc ccataggacg ttttccagat ataactgtca accagcttcc
      421 ttccggtgta ctggcttcgg ctctggaaat aggctgaaaa tcttccggac atggagaata
      481 cattgtcttc atataattaa gtcgtctgga tttctcatta ataaactcct ctatttttaa
      541 ttgtgccata cttctggtta taccagagat atacttcttg tcatatatgt ttggtgattc
      601 attaaagtaa ccatgttcaa ctctaggata aatatttctc actatatatt cagtatttgc
      661 agtatttatt ggaaatacgt tcttaatatt cttgatggga ctaatcattt taatctctat
      721 actcaaatat aaacattacc atgaacaata taattgttcc taagtggaat gtctccacgg
      781 agaacacttt atctatcggg aaatagtgcg ggcaacagtg tttaattata tcgagtgcaa
      841 ttacaattgc tggcgacggc accgttggtg tggcggcctc agtccggaac aatttgaaaa
      901 ccagaacctc gcttaggcct gtgtccatat tacgtgggta ggctcacaga gcctcactta
      961 cgactgcgtc catattatgt gggtaggatc aataaagacg ttttatatgt gtctgcttgc
     1021 agccagaaaa tttataccct gcaggtatca taaacatgct tgataactta attggggctc
     1081 cctcttcctt ttatggtgta attgtttgat tgaatacttc cggggatttc agtatgaaat
     1141 gaagtatatt taatatactt tacaaaaagt ttgtattagt ggaataagga caatagctta
     1201 tccaaactgc caagcctctt tattataagt aagatctggc actggaatat tctcaaaagg
     1261 actaacatca tcaccacgac cttcaaccaa ccattttatt gcatttaaat cccagaatag
     1321 ttcagtagtg gtcaatttgg gaaacgcaca atattgtacg gttacgttat cgcaatagag
     1381 tgaactctcg ccctatacgg cgttttcaag gattagtcct taatcggaca accacattaa
     1441 agcataataa ataattaagc tttaataata tactatagat acagttctat tataaaaaat
     1501 actattttag gtgatgcatt aaattagtag aatatttatt cttttataaa agcaccaatg
     1561 aattgtccaa gtaaacatga gataaaatgt gtagaggata actgagtttg gtcactttaa
     1621 ctgtattagt cgcagtctga ctctcgactt taaaaggatg ggaattacct ggactgatag
     1681 tgagaaagct ttccattgaa ataagtcaaa taaaagttga tggcttcagt gctaaagtga
     1741 ggtgcccgct agccgtattc actcgaatcg gaattcaatg tattgcttct gccatttcca
     1801 taatatttca cgggaaaaac aaaatctgaa aaactatact taagtgttgt attgagtcag
     1861 ttgagataca aatacatatt tatcagcaca cgttacgtca aatacaggag gttgtcatgt
     1921 tggtactgaa aggcacgttg gctattgcag ccgtgatgac aatttattgt cttatcgttg
     1981 ttcttatgga acgcttttct gattgatttc atattggcga gagaacaaga aggtgagtag
     2041 aattgtcgcg gaatcctgaa actatcagcc tcaggtatga acaccaaaag gtaggtagag
     2101 aaatctatgg cccccgttac tttattcgca caatgaggta tttttttatt tttacaaaag
     2161 ttcaataaat attataataa aagatatgtt cttggtaaca taaacaaata tttaattaaa
     2221 ataatgcttg gcattgacgt ttatcttata aagtaagaag atcatggaca tttcaaccaa
     2281 aaaagtagag ttctcgatga aattaaaatt ctttgtactt gcactttgtg tacctgcgat
     2341 ctttactaca catgctacca ctaactatcc actttttata ccggacaaca tcagtacgga
     2401 tattagtcta ggatctctga gtggcaaaac aaaagaacgc gtttatcatc ccaaggaagg
     2461 ggggagaaaa attagtcaac tggactggaa atacagtaat gcgactattg ttagaggtgg
     2521 catcgattgg aagctaattc caaaagtgtc tttcggagtt tccggttgga ctactttagg
     2581 taaccagaaa gcaagcatgg ttgataaaga ctggaacaat tccaataccc ctcaggtatg
     2641 gaccgaccag agctggcatc ccaatacgca tctccgtgat gctaacgaat tcgagctgaa
     2701 tcttaaaggt tggttattaa ataatttgga ttatcgactt ggactaatag caggttacca
     2761 agagagtcgt tacagtttta atgcaatggg agggagttat atttatagtg aaaacggggg
     2821 gagcaggaat aaaaaagggg cacatcctag tggtgaaaga acaataggtt acaaacagct
     2881 cttcaaaata ccttatattg gattaactgc taattaccgc catgagaatt ttgagtttgg
     2941 agcagaactg aaatatagtg gttgggttct ttcatctgat acagataaac actatcagac
     3001 tgagacaatt tttaaagatg aaataaaaaa ccaaaattac tgctctgttg ctgcgaatat
     3061 tggatactat gtcaccccca gtgcaaaatt ttatatagaa ggctccagaa attacatttc
     3121 taataaaaaa ggtgatacat ctctttatga gcaaagtacc aatatatctg gcaccattaa
     3181 aaatagtgca agtattgaat atattggttt tctcacttcc gcaggtataa agtatatttt
     3241 ttgaattggt tcaccgagtt gattacgttt tcatggctaa aaattgccgc ccattgtgag
     3301 tcatattcgc cctgactttc cagaaccata cgaactgccc gttgacggac ttcgggggaa
     3361 aaacgtgtat ttttagtcat tctgtttacc tctttctcag ggcgtttagt ctccaggatt
     3421 tccggggcgg ttcaaaaaac tcttcgttga ttactgtgga cttactgttg gcattaccga
     3481 ccctgagacc ggagaaataa gaaccgctta gatcatcgtg gctgttctcg gggtatcaag
     3541 ttacacatgg gcagaggcta cccaaggtct actattatcg cctgaaagcg ctgatggaac
     3601 agtgctatca gggacatgct gatgaaaata cctaaataat cagccagcac tctatctttc
     3661 caaatccaca gcatagcaaa gagagcaaaa gagcctgtaa attcagaaat taaggaaacg
     3721 cctcttggtg tacataatct atcttatgtt aaatgaaatc taatttcttg attatatgct
     3781 ggatcgttcg tgggtggctt tgcccctata tttccagaca cctgttatca ttggcccgct
     3841 gccgcagata ttcccgtggc gagcgataat ccagcgcact atgcggatgc cattcgttat
     3901 aatgctcgaa tgcctctgca aggttctttg ctgccgttaa cccgtcaggt ttgggcatga
     3961 tactgatgta gtcatacttt attgttttca cgaagctctt tgctattctg ttactctccg
     4021 gactccgcac cgccgtgttc ttcggttcaa caaagtccca acatccgggt gaactggcgt
     4081 gtttcattag ctcggtagca tgaaccatta tccgtcagtc acttcactgg agacgccgga
     4141 agctcgttgc cgaagcggcg ttccaccgct cccagcatgt cgccaacggg agagcatcta
     4201 cggtctgatt gaactgttta ttcaaattgt ccaccgtctg tcagtacgct ctgaacgtcg
     4261 gctggttaaa acactgctcg ctgattttca gagggttcac ggtaaaacag cacttttatt
     4321 ccggattgcg gaagcggctc tgaataatcc ggatgggctg gttaaagagg ttgtgtacca
     4381 tctgcatatt gttgaaccag atcgcagcgg taagagaagt tcatcatact tggcgcagtt
     4441 acgcgatgtt tctgctagag gtgatgccgt aaagaacggc agaacattac cggaacaaga
     4501 tagcggtcta cctgcactgg tatcagaccc ggggcttccc cggatgatct ccacggtact
     4561 ttaggtactg tgtctatatt atgtaggtag gatcaattaa ctttgtagtt gaaattcttt
     4621 cattgaaata atgaataaaa aagacaaacc gcattaaaca atgccagaat ataattgcat
     4681 ttttgttccg tttttttaaa tcatagtata caaagaagct tgcatactat atgaaaatat
     4741 aacacccttt actatgatgt ttataaaaca atatatggag tcatgtaggt atgaatttag
     4801 atggtgttag accatactgt agaatagtca ataaaaagaa tgaaagcata tcagatattg
     4861 catttgcaca tataataaaa agggtaaaaa attcatcatg tactcaccca aaagcagcat
     4921 tggttttttt aggagagaaa ggtttttgtg atagcaatga tgttctatct attatgggac
     4981 aacaaatacc aagagtattt aagaacaaga tgttatatga ttatgttttt aaaaatgaaa
     5041 aaagtaaaaa tgattttcta aaaatggctg aatcatggct accacagagt gaaccaatag
     5101 taataaataa tgatgatgac gcattgaatg ctgctgctta tttttctgta aaaaaagcga
     5161 aaataaaaac agtaaacgat actgatttta aagagtataa taaggtttat attcttgggc
     5221 acggtagtcc tggttctcat caattaggcc ttggttcgga acttattgat gtacaaacaa
     5281 tcatttcaag aatgaaagac tgtggtattc taaatgtgaa agatatccgt tttacttcat
     5341 gcggctccgc tgataaagtg gctcctaaaa attttaacaa tgcccctgct gaaagtcttt
     5401 cttgtatcct taactctctg ccttttttta aggaaaaaga atctttgcta gagcagataa
     5461 aaaaacacct tgaaaacgat gagtcattga gtgatggtct aaaaatatcc ggctatcatg
     5521 gatatggagt tcactatggt caagagcttt ttccctactc acattatcgt tcaacttcaa
     5581 ttcctgctga tccggagcat acagtaaaaa gaagctctca gaaaaagact tttattatta
     5641 ataaagaact ggattagtat aaaattttta acctatagtt aattgtttgc atttaagatg
     5701 aagttatttt atctttagta gaaaagtgct tccctatgag ggaaaatcac aaatcatcat
     5761 aatacagaga caaaacgata cgaaaaaata gataaaaaac atcattcttt taccacatta
     5821 ttttccgtga atatgaaaaa taatgttatt actttaatat aagactattt tttgtttttc
     5881 catcactctg ttcaaatttt tccgcatgac ttgtgttttt tgtaatacag ctcgtttttt
     5941 acagctgacc aaaatcatca attaattatg ctaaggaaat aaattatgaa aaccaaaaac
     6001 tttcttcttt tttgtattgc tacaaatatg atttttatcc cctcagcaaa tgctctgaag
     6061 gcagaaggtt ttctcactca acaaacttca ccagacagtt tgtcaatact tccgccgcct
     6121 ccggcagagg attcagtagt atttcaggct gacaaagctc attatgaatt cggccgctcg
     6181 ctccgggatg ctaatcgtgt acgtctcgct agcgaagatg catactacga gaattttggt
     6241 cttgcatttt cagatgctta tggcatggat atttcaaggg aaaatacccc aatcttatat
     6301 cagttgttaa cacaagtact acaggatagc catgattacg ccgtgcgtaa cgccaaagaa
     6361 tattataaaa gagttcgtcc attcgttatt tataaagacg caacctgtac acctgataaa
     6421 gatgagaaaa tggctatcac tggctcttat ccctctggtc atgcatcctt tggttgggca
     6481 gtagcactga tacttgcgga gattaatcct caacgtaaag cggaaatact tcgacgtgga
     6541 tatgagtttg gagaaagtcg ggtcatctgc ggtgcgcatt ggcaaagcga tgtagaggct
     6601 gggcgtttaa tgggagcatc ggttgttgca gtacttcata atacacctga atttaccaaa
     6661 agccttagcg aagccaaaaa agagtttgaa gaattaaata ctcctaccaa tgaactgacc
     6721 ccataaagct ggacagcctg tatcaggcta tggagggccc atagacaaat ctaccctata
     6781 tgagcatagg aggagtctat gggcacacca cgttttaccc ctgaatttaa gggattactg
     6841 gaaaggctgg gacatatccc tccggcagaa gcagaaaaag cttattatgc tgccatcgga
     6901 aacgatgatc tggcaacctg agttcacaga taaaacattc tctaggaaac tcggggcggt
     6961 tccgttcacc acatgcaatg tggtgttgca ggggaacggt ctgcaccatc ccctatgtcg
     7021 atcaatataa cagaaatgac aacttcagat tcagggcaca acctaaatat attttaggtc
     7081 acctctcaaa tcgtttgcct gataccgctc cattttttaa taaaaaaatc aatcattttt
     7141 gaatctttgc ttttcattgc attgtcaagc atagtgtccc cactattt
//
LOCUS       DAJTRB010000161         7178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTRB010000161 DAJTRB010000000
VERSION     DAJTRB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.161"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(295..1197)
                     /locus_tag="OO330_003945"
     CDS             complement(295..1197)
                     /locus_tag="OO330_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5884619.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1276..2229)
                     /gene="lsrR"
                     /locus_tag="OO330_003946"
     CDS             complement(1276..2229)
                     /gene="lsrR"
                     /locus_tag="OO330_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5884620.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2478..4013
                     /gene="lsrA"
                     /locus_tag="OO330_003947"
     CDS             2478..4013
                     /gene="lsrA"
                     /locus_tag="OO330_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5884621.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4007..5035
                     /gene="lsrC"
                     /locus_tag="OO330_003948"
     CDS             4007..5035
                     /gene="lsrC"
                     /locus_tag="OO330_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5884622.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5035..6027
                     /gene="lsrD"
                     /locus_tag="OO330_003949"
     CDS             5035..6027
                     /gene="lsrD"
                     /locus_tag="OO330_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5884623.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKELLARRANNPLP"
     gene            6039..7056
                     /gene="lsrB"
                     /locus_tag="OO330_003950"
                     /pseudo
     CDS             6039..7056
                     /gene="lsrB"
                     /locus_tag="OO330_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7083..>7178
                     /locus_tag="OO330_003951"
     CDS             7083..>7178
                     /locus_tag="OO330_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5884624.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGAL"
BASE COUNT         1670 a         1813 c         1875 g         1820 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtcctctct gactaaatag
      241 acacgtccgc gctacagtct caccacagcg atcactgctt tcaaaggatg ggactcatga
      301 cggcatacgg aacggagtaa tcggcaactg atgccgggcc tcgctgagcg tgcactgctt
      361 attatccgtg aacgtgccgc caagaaccgc ggtttgtgcc ggacgcacaa cgcctaaccc
      421 aaggcaacca agctgcacgt cgcctcctcc aacgaccacc ggagtgcccg ccttcagacc
      481 gcagagttcc gccgcttgtg aacttaccac gcccagcaat gtgccggttt ctttgacagg
      541 agaaagaata tcggcacgca ggccagccat atccagcaat gcaggtttcc aggcacgggt
      601 ggttagatca agaagtcccg tggtgccagc gttagaggga tccaccgcca gttcgccgct
      661 gagcatatag gccagccagt cgctgatcat ggtgatggtt gatgcctgac ggtaaatatc
      721 ggaacgatgg tgcgccagcc aaagtaatct ggggatggca cttaaagcca gtgtttgtcc
      781 ggtcgctcga taaacttcgt tttcaaaggt attgttgtgc agttctttaa gttcgctaac
      841 ttcgcgtgcc gctctggcat ccacattggc gcaggcccag atcggtgttc cttcattgtt
      901 atataaaaca atgccttcac gcatagaaca tgccgaaacg gcagcgatat actccgggtc
      961 tatgccggcg ttgtgcagcg cctggcgcat acactcacac gccagttgcc agtttttgtt
     1021 gagatcaaat tccatagaac caggaacgtc cggtactgcc agatgccgcc actccgcctg
     1081 tcccactgct atttgattgc cttccaggtc gaatatcaca gcccgaatac ttccggtgcc
     1141 tgcatccagc gccatcaggt agtactttga ttctgaaggg gtaaagagtc gagccattag
     1201 attatcctcg gcttatgcgt tctgtctgtt cctctatacg ttctccatca ttcccggtaa
     1261 taaggtcatg caaatttaac tacgtaaaat cgccgctgct gtgtcctgat cggtaaccag
     1321 tgcgttgata taaccgcctt tcattgcagc ggcaattgct tcggctttat tttctccccc
     1381 tgccacgcca acccggacgg gtatggtctt cagcgcgctt aaaggtaagc caatcagttc
     1441 gttatgtatt ttgatatccg tgacaacgtc accttttgca tcaaaaaagt agcctaaaat
     1501 gtcgccaacc gccccttttc ggccaatcat taactgttcg ccctggctga tataaccgga
     1561 gcgaatgatt gtcgcgtcgt cttgttgact cacggctcca atgccgacaa tcgccacatc
     1621 cgctgcttgc gcggctagca gaacatcttt gacgcaattt tcatttttta gcgtacgggc
     1681 aatgtcagcg gaggatgccc gcaacggagc cggaataata ttcacactgc acgccgcgtt
     1741 aagctgcccg atacccgtca tataagaacc gacgccaccg gagagcgtga ccaggcgaat
     1801 ttgctgtgac gaaataaaac cacttaagcg ttgcagcgta ttcatggttg cctcgccaaa
     1861 accaatcgcc agcatctgtt gtggttgaag taaactcatc aacatatgcg ccgcgcctat
     1921 ccctagtcgc ccgccgacat cagcatccgc aagcccaggg atcacccgga catgttgcag
     1981 cgaaaactga cgacgtaatt gagtttcata ttccagacag ccttcaaagc gagaattaat
     2041 ctgtacgcga ataatgccgg actgatgccc tttctccagc aagcgcgaca ctttcaaacg
     2101 ggtcaggccg agacgatcgc tgatctcgct ctgggtcagc ccgtcgtgat agtaaaacca
     2161 cgcgatccgc gcgacctgtt cttcttcaca cattccctgt tctgaaattg ccgaatcgtt
     2221 gattgtcata attcattctt cactttgagc atatttaaat ctttaatgca attgttcagt
     2281 tcttgcgcat ttatatctgt gatggcaacc acagtttgac tctacgagca tgaacaaacg
     2341 caaccgtgaa aatcaaaata gcataaattg tgatctattc gtcggaaata tgtgcaatgt
     2401 ccacctaagg ttatgaacaa attaaaagta gaaatacatt tgttcaaaac tcacctgcaa
     2461 aactgaacgg gggaaatatg caaacgagtg atacccgcgc gttaccgcta ctttgcgccc
     2521 gctcggttta taaacagtat tcaggggtca atgtcctgaa aggcatcgat tttacgttgc
     2581 atcaggggga ggtccacgcc ctgctcggcg gcaatggtgc cggtaaatcg acgttaatga
     2641 agattattgc cggtattacc cctgctgata gcggtacgct ggagattggg ggcaacaact
     2701 acgccagatt aacgccagtt catgctcatc agctgggcat ttatctcgtt ccccaggaac
     2761 cgctgctttt cccaagcctg tcgataaaag aaaacatcct gtttgggctg gcaaaaaaac
     2821 agctctccat gcagaaaatg aagaacttgc tggcggcgct gggctgccag tttgatctgc
     2881 atagtctggc aggatcgctg gatgtcgccg atcgccaaat ggtggaaatc ctccgcgggc
     2941 tgatgcgcga ctcgcggatt ctgatcctcg atgaacctac cgcctcgctt acccctgcgg
     3001 aaaccgaacg cttgtttagt cgcttgcaag agctgcttgc tactggcgtg ggtattgttt
     3061 ttatctcgca taagctgccg gaaattcgcc agattgccga tcgaattagc gtgatgcgcg
     3121 acggaaccat cgccttaagc ggcaaaacca gcgaactgtc taccgacgac attattcagg
     3181 ccatcacccc agcggtacgg gaaaaatcgc tctctgccag ccaaaaatta tggctggagt
     3241 tacctggtaa ccgcccacaa catgccgtcg gaacgccggt gctgacactg gaaaatctga
     3301 ctggcgaagg cttcaggaat gtcagcctga cgctcaatgc cggagaaatt ctgggcctgg
     3361 ctgggctggt gggagccgga cgcacagaac tggccgagac gctctatggt ctgcgtactt
     3421 tgcgtggcgg acgcattatg ctgaatggta aagagatcaa taaattatcc actggagaac
     3481 gtttactgcg cggtctggtt tatctgccgg aagatcgcca gtcatccgga ctgaatctcg
     3541 atgcttcgct ggcctggaac gtctgcgccc ttactcataa ccttcgtgga ttctgggcga
     3601 aaaccgcgaa agataatgcc accctggaac gttatcgtcg ggcgctgaat attaaattta
     3661 accaaccgga acaagctgca cggacattat ccggtagcaa ccagcaaaaa atcctcattg
     3721 ccaaatgcct ggaagcttcg ccgcaagcat tgattgtcga tgaaccgacg cgcggcgtgg
     3781 atgtctcggc ccgtaatgat atctaccagc tgttgcgcag catcgccgca cagaatgtgg
     3841 ctgtgctgtt tatctcctct gatatggaag agatcgaact gatggcagat cgtgtgtatg
     3901 tgatgcatca gggcgaaatt acccactctg cactgaccgg gcgcgatatt aatgtcgaga
     3961 ctattatgcg cgttgccttc ggcgatagtc agcgtcagga ggcatcatgc tgaagtttat
     4021 tcagaacaac cgtgaaatca cggcattgct ggcggtggtg ctgctgtttg tattacccgg
     4081 ttttctcgac cgccagtatt taagtgtgca aacgctgacc atggtttata gcagcgcgca
     4141 aatcctgatc ctgctggcaa tgggcgcgac gctggtaatg cttacgcgca atattgatgt
     4201 ttcagtgggt tcgattaccg gaatgtgcgc ggtgctgttg gggatgttac tgaacgcagg
     4261 atattcacta cctgttgcct gtgtcgcgac tttactgctt ggtttgctcg cgggattttt
     4321 caacggtgcc ctggtcgcgt ggctaaagat ccctgccatt gttgccaccc ttggcacgtt
     4381 agggttgtac agaggcatca tgttgctgtg gactggcggt aaatggattg aagggttacc
     4441 cgccgaactg aaacagctct ccgccccgct gctgcttggc atttcagcaa ttggttggtt
     4501 gacgataatt ctggtggcat ttatggcctg gctgctggcg aagacggcgt ttggacgcag
     4561 tttttatgcc acgggcgata atttacaggg cgctcgtcaa ctgggcgttc gtacggaagc
     4621 cattcgcatt gtggcatttt cgttgaacgg ctgcatggcg gcactggcgg gaattgtgtt
     4681 tgcttcgcag attggtttta tcctcaacca gaccggtacc gggctggaga tgaaagcaat
     4741 tgcagcctgc gtgctgggcg gcattagttt gctcggtggt tccggtgcgt tcatcggtgc
     4801 ggtactcggc gcatggttcc tgacgcagat cgatagcgta ctggtgctgt tgcgcattcc
     4861 ggcatggtgg aatgatttta tcgcgggtct ggttctgctg gcggtgctgg tgtttgatgg
     4921 acgcctgcgt tgtgcgctgg aacgtaatct acggcggcaa aaatatgccc gctttatgac
     4981 gccaccgcca tccgttaaac ccgcttcgtc aggtaaaaaa cgggaggccg cataatgcgt
     5041 attcgctacg gttgggaact ggctcttgcc gcactgctcg ttattgagat tgtcgcattt
     5101 ggtgcaatta acccgcgaat gttagatctc aatatgttgc tgttcagcac cagtgacttt
     5161 atctgcattg gcattgtcgc tctaccgctg acgatggtga ttgtcagtgg cgggatcgat
     5221 atttcgtttg gttcgaccat cggcctctgc gccattgcat tgggcgtact gtttcaaagt
     5281 ggtgtgccga tgccgctggc gatactcctg accttactgc tcggcgcatt gtgcgggctg
     5341 atcaacgccg gattaattat ctataccaaa gttaacccgc tggtgattac gcttggcacg
     5401 ctgtatctgt ttgccggaag cgctctgctg ctttccggta tggccggagc gacggggtac
     5461 gaaggtattg gtggattccc gatggcgttt acagatttcg ctaacctgga tgtgctggga
     5521 ctccccgttc cgctgattat cttcctgata tgtctcctcg ttttctggct ctggctgcat
     5581 aaaacccatg ccggacgtaa tgtgtttttg attgggcaaa gcccgcgcgt ggcgctttat
     5641 agcgcgattc cagttaaccg caccttatgt gcgctctatg ccatgacggg gctggcgtct
     5701 gcggtcgccg ctgtgctgct ggtatcgtat tttggttcag cacgttccga tctcggtgcg
     5761 tcgtttctga tgcccgccat caccgccgtg gtgcttggcg gggccaatat ttatggtggt
     5821 tccggttcca ttatcggcac cgccattgcg gttttattag tgggatattt gcagcaaggt
     5881 ttgcaaatgg caggagtgcc aaatcaggtg tccagcgccc tttccggtgc gctgcttatc
     5941 gtcgttgtcg taggtcgttc cgttagcctg catcgccagc aaattaaaga gttgctggcg
     6001 cgtcgggcca ataacccatt gccataaagg atatcttcat gacacttcat cgctttaaga
     6061 aaatcgcctt acttagcgct cttggcattg ccgcaatctc tatgaatgtg caggccgcag
     6121 agcgtattgc atttattccc aaactggttg gcgtgggatt ttttaccagc ggtggcaacg
     6181 gcgcacaaca agcgggtaaa gagctgagcg ttgatgtgac ctacgacggg ccgacagaac
     6241 ccagtgtttc tggtcaggta cagttgatta ataacttcgt caatcaaggt tataacgcca
     6301 ttattcgttt ctgcggtttc gcctgatggc ttgtgtccgg cactgaaacg cgccatgcaa
     6361 cgtggtgtga gagtgctgac ctgggactct gatactaaac cggagtgccg ctcttactac
     6421 attaatcagg gaacgcccgc ccagttggga ggtatgttgg tggatatggc ggcgcgtcag
     6481 gtgaataaag acaaagccaa agtcgcgttt ttctactcaa gccccaccgt tacggaccaa
     6541 aaccagtggg tgaaagaagc gaaaatcgcc aaagagcatc caggctggga aattgtcact
     6601 acgcagtttg gctataacga tgccactaaa tcgttacaaa ccgcagaagg aatattaaaa
     6661 gcgtatagcg atctcgacgc cattatcgcc cccgatgcca acgccctgcc cgctgccgca
     6721 caagccgcag aaaacctgaa aaatgacaaa gtagcgattg tcggattcag tacgccaaat
     6781 gtgatgcgcc cgtatgtaga gcgcggcacg gtgaaagaat ttggactgtg ggatgtggtt
     6841 cagcaaggca aaatttcagt gtatgtcgcg gatgcattat tgaaaaaagg atcaatgaaa
     6901 acgggcgaca agctggatat ccagggcgta ggtcaggttg aagtctcgcc aaacagcgtt
     6961 cagggctatg actacgaagc ggatggtaat ggcatcgtac tgttaccgga gcgcgtgata
     7021 ttcaacaaag agaatatcgg caaatacgat ttctgatgtg cattacttaa ccggagtaag
     7081 ttatggcaga tttagacgat attaaagatg gtaaagattt tcgtaccgat caaccgcaac
     7141 aaaatatccc ttttaccctg aaaggttgcg gtgcgctg
//
LOCUS       DAJTRB010000162         6851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTRB010000162 DAJTRB010000000
VERSION     DAJTRB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.162"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..1235
                     /locus_tag="OO330_003952"
     CDS             <1..1235
                     /locus_tag="OO330_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR5884625.1"
                     /translation="KKIRNIMIVVFLLSLLIPISPINAFFSSSYKVYSEAKEYNMSPH
                     EISDIKSKYTKEIVTNDSYSHEIINGNGKNILVIFIEGMSSLVISKELTPNLYEFRKK
                     SISFNNYYNHTAATFRGLRGQLTSSYQMTGGYHKDNSGLGQASKVEIEHKLNTYNYIT
                     KLPIILEENGYHTYFQASNSIDDPLSLMLSTLKFNHIYGREDYNKKSGELTDKESFDL
                     LFKKLNEAKQPFFYGVYTVGTHLGLDSPDVKFYDGRNEYLNHFHQLDHWFGDFISHFN
                     KTSLSKDTIIIITSDHASYPSEDYKKTFKTDNTFFVDQIPLIIWSNGVEFKDINAHGL
                     NSLSLTPTILDILGIKESHNMFIGESLFEKEKNKNELNCTSAIGDLYVKTCNGKMEIE
                     KRKEKTELIKQLQLFGDR"
     gene            complement(1304..2114)
                     /locus_tag="OO330_003953"
                     /pseudo
     CDS             complement(1304..2114)
                     /locus_tag="OO330_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009652796.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(2182..2418)
                     /locus_tag="OO330_003954"
     CDS             complement(2182..2418)
                     /locus_tag="OO330_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5884626.1"
                     /translation="MTEMERQLLSALESLQTSYEQQQQAWQDSYSSVQRMFETTSQAL
                     ERNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
     gene            3720..4036
                     /locus_tag="OO330_003955"
                     /pseudo
     CDS             3720..4036
                     /locus_tag="OO330_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4433..4801)
                     /locus_tag="OO330_003956"
     CDS             complement(4433..4801)
                     /locus_tag="OO330_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884627.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYG
                     LNISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAE
                     EESQRVVESFRDSLSRKKGF"
     gene            complement(5186..5335)
                     /locus_tag="OO330_003957"
     CDS             complement(5186..5335)
                     /locus_tag="OO330_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884628.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMVQVTEENLHAPVAFGPPV
                     GREAL"
     gene            complement(5580..6271)
                     /locus_tag="OO330_003958"
                     /pseudo
     CDS             complement(5580..6271)
                     /locus_tag="OO330_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1933 a         1541 c         1476 g         1901 t
ORIGIN      
        1 aaaaaaaaat aagaaatata atgattgttg tttttttatt atcattgtta ataccaatat
       61 ctccaataaa tgcttttttt tcttcatcat ataaagttta ttcggaagca aaagaataca
      121 atatgtcacc acatgaaata agtgatataa agtcaaaata cacaaaagaa atagtaacaa
      181 acgacagtta ctcacatgaa ataataaatg gaaacggaaa aaatatttta gtaatattta
      241 ttgaaggtat gtcttcattg gtaatatcta aggagctaac cccgaattta tatgaattta
      301 gaaaaaaatc aatatcattt aacaactatt acaaccatac cgcagctaca tttagaggtt
      361 taagaggaca gttaacctca tcatatcaaa tgaccggtgg gtaccataaa gataattctg
      421 gccttggcca agcatctaaa gtagagatcg aacataaact aaatacttac aattacataa
      481 caaaattacc aatcatactt gaggaaaatg gttaccatac atattttcaa gcatctaatt
      541 caatagatga cccattgagt ttaatgctat caactttaaa gtttaatcat atatacggaa
      601 gggaggacta taacaaaaag tcaggtgaac tcacagacaa agagtctttc gatttattat
      661 ttaaaaaatt aaatgaggct aaacaacctt tcttctatgg tgtttatact gtaggtacac
      721 accttggctt agatagccct gatgtaaagt tttatgatgg gagaaatgaa tatttaaatc
      781 actttcatca attagaccat tggtttggtg attttatatc tcattttaat aagacaagct
      841 tatcgaaaga tacaattatc ataatcacaa gtgaccatgc aagttaccca tcagaagatt
      901 acaaaaaaac attcaaaact gataatactt tttttgttga tcagattcca ttaataatat
      961 ggagtaatgg tgttgagttc aaagacatta atgcacacgg tttaaattca ctatctttaa
     1021 cgccaacaat actagatatt cttgggatca aggagtcaca caacatgttc ataggtgagt
     1081 ctctatttga aaaagaaaaa aataaaaatg aactcaactg tacatctgca ataggtgacc
     1141 tgtatgtgaa aacgtgtaat ggaaaaatgg aaatcgagaa aagaaaagaa aaaacagaat
     1201 taataaaaca attgcaactt tttggtgata gataattaaa aggcggtttt accccatgtt
     1261 gattattagt ccacgtgggg ttagtttaaa atgatagtaa aacttacttt ttttcattct
     1321 tgagccgcct gtagaatgtg gctcgactaa cacctaaaga ctcccattct ttttttaaag
     1381 ttataggaat taatattaaa actaccttta ggccgtcctc ctgaattgct tagctttcct
     1441 cctttacccc ctctctttct ttgaatttct gaacggtgcg tattttttac atactcaata
     1501 aaacgatcct ctgaaaaatt attcgctgtc cattttgaga tgctttttga tattccaaga
     1561 acttcatttt cacttaatgg tgatacgaat tgtaaattgt aggcacaggc tcgttcatag
     1621 caggcttgta accactgctc atactcaggc cacccctgac ggatggcacg atatgcccac
     1681 ttgcgggttt tgtcgaacag agtgcagtta cggcctaaac cataatcggc ggcaattttc
     1741 cagtgactgt ggttcggatt cttgcaaatt agcccagaat atcccgtgtc agctcccaat
     1801 tttctacgta gcgcattctc tacggcagct gcgtagcgta acggtttcat ttttccgtca
     1861 ggtgctgtgc gtatagaggt ttccagcgca taaagcaggt gcgcgtgtcc gttttcgggg
     1921 ttggtgatgg tcagcgtagg tgcgggagcg ttgcggtcgc tccagtcaat ggctgcgccg
     1981 atgcggtcaa catcaaatcc cagccagaac atggcgtgag gctgattgaa ctggatgtat
     2041 ttcgccagaa tagcgcgttc tttccctgcg atgcgcacac caaagtgcaa atcatccgag
     2101 aagtacggct tgtgtgatac atggactgac ctcgatagcg ggttgcttgc ttctcctgct
     2161 gtaactgccg ttcctgctgc tttacgagcg tgttaatcgg ctcagatcgc tgctcaagcg
     2221 ttcgacctgt tcgtgcaagg cgtttacctg attgctcaag ttcagacata ccctgtcgtt
     2281 gcgctccagt gcctgtgagg tagtctcgaa catgcgttgc acgctgctgt aactgtcctg
     2341 ccacgcctgt tgctgttgtt cgtaacttgt ttgtaagctt tctaaggcgc tcagaagctg
     2401 cctttccatc tcggtcatgg ttttgcctgt cctcagtgat aaaaacgtgc tgtggctttt
     2461 ctgtgggctc tgacggggtt atttcgaggt gtacttcatc acggtgtttc cggctgtcga
     2521 tgtataggtc tcggtgccat gcgctttctt cggcagtacc accagatagg tgttcttctg
     2581 cttgtcgtcc ttgtaaatcg ctacccctgc gccgctctgg gctttcaggc tctccagttg
     2641 ctggctgtac ggtcacaccg ttcaagcaga cgctgatgct cagtctcggt catgcggatt
     2701 ttgatttctt tcgttcgctt ttccataggc tccgctgaga aaaaattcgc accactgtga
     2761 gaaagtgctt catgtggcag gacaaaaaag gcggcaccag tgcgtcagca gaatatgtga
     2821 tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     2881 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaaaatactt
     2941 aacagggaag cgatagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
     3001 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga cttaaagata
     3061 ccaggcgttt ccccctggtg gctccctcgt gcgctctcct gttcctgccg ttcggtttac
     3121 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     3181 ggaaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct
     3241 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacacgac aaagcgccac
     3301 tggcagcagc cactggtaac aggattagca gagcgagtta atgtaggtgg tgctatagag
     3361 ttcttgaagt ggtggcctaa ctacggctac actggaagga cagtatttgg tatctgcgct
     3421 ctgctgaagc cagttaccag gttaagcagt tccccaactg acttaacctt cgaaaaacca
     3481 cctccccagg tggttttttc gttttcagag caagagatta cgcgtagaaa aaaaggaact
     3541 caagaagatc ctttctatgc tcaaccaccg catggctcat agccgcgctg gccgttgccg
     3601 gtcagctcca gaatcctcga ccaatatgga accttgcccc tcccaaaatt caactaaaaa
     3661 accctcattt cctgccgata aaatgggtat ctcttaacta tgcctaaata aggataattt
     3721 tgaaaaaaat attcatccta tcggcagcgc tgatcatggc aggatacgca acaaagcaat
     3781 atccacaggc cgcgaacgtc accggtccag aatcttccgc aatggattgc aaagcggtag
     3841 ctctggaaat cgccaaaacg cgcagtgtac aacaagaaat tgaggaaacc ggcgaatttg
     3901 acgggcgcac cgtattaggt ttcctgggtg attttgggat aggaaatggc atggcaaaag
     3961 gcgaagcccg caaaaagcag ctaaccggct aaatcagctg gaagccctga aggctgcaaa
     4021 gtgtgcagat caataaggta caaaaaagcc ctgcggggct tttttgtacc tgccaatcag
     4081 ttggatttac cataacatcc gttgcgtgca ccgcctgaga ggctctgaga gcgtttctga
     4141 cgggaaaccg gcaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc
     4201 tgtttttacg gcatgcatga gtatgcacgc aagtgcatag cgcgaaaatt caccccattc
     4261 agccagggta agtcctgttt ccggcattct tcccatccga aaacggtgaa cggggacgca
     4321 atttattgcg tggccgtgag gcattgacca agccgtaagg cacggtaggt aattgcaggt
     4381 atccgacaat ttcgagtaga ggatccacta tttcactact tagaattcaa aatcagaatc
     4441 ctttcttcct gctaaggcta tctctgaatg actcaaccac tctttgagat tcttcttctg
     4501 ctcgcatgaa gtcattcccg gacagaaaaa aaccattgtc aggcccactg gcttcagaga
     4561 aattcgtttc tgcagcagac acagaatcca catcagcaag agacggttca tctgtacttt
     4621 ttctcaatct gtaaagagcc ttatcaaacg caaccagtga gatattcagg ccgtaacgtt
     4681 cattcagcac agtcacaatc tcttccctac ggataccatt tttcttccag gtttctatat
     4741 cgttcttgga atccctgaca gccgcaatgg ctgttcttgg accagggtta aaattcgcca
     4801 tataccgccc ataatgtctt aaatttgtct cataatagtc caaaaataag attgaaacgc
     4861 aaaaaaatgt cttacaattg tcttattgtt gtcctttccg gttcatgtat ccatgaaccg
     4921 cctgatgtgg aacgcttttt atcgatctca tgcgcagcca aagatagatt cattctttaa
     4981 aagtcaggtt acattatccc tgaaatggtc atacaccata cgcaaccgcg attttcatcg
     5041 caattgcatg aattaaaagg taaaaactga agtccccgtt tatttatgta atacgcaacg
     5101 tattacaacc acctgtaatt acatgaaaat aaagaaataa gatgtaatac cggcgtatta
     5161 ccacgtacgc gggtaagcga aaaagctata acgcttcccg gccgacaggc ggcccaaaag
     5221 ccaccggggc gtgcaggttc tcttcagtaa cctgcaccat taaatcaaaa aggtcatatt
     5281 cgccgggaag ctgcgtatca gtcgaatcca gttgttcatc atgttcagat ggcatggggt
     5341 gatatccagt agctgagagg tggttagcgg gtttcggggc gcagccctaa accagtcacg
     5401 tagcgctagc ggagtgtata ctgacttagg cggtagtgta gtatgacaga ttctgtggtt
     5461 acggcaaagt aacagccctg tctaggagag ggctgttatc tgatataggc ttgttttttt
     5521 ctcaccgttt ttgcgagcgc tttttcctgc caagaggtag gtagggtaat gaatccaact
     5581 tactgatagt gttttatgtt tagataatgc ccgatgactt tgtcatgcag ctccaccgat
     5641 tttgagaacg acagtgactt ccgtcccggc cttaccagat gctgcctcag attatgccgc
     5701 tcaattcgct gcgtatatcg cttgctgata acgtgcagct tttccttcag gcgggattca
     5761 taaagcggcc atccgtccgt catcaatacc accacgtcaa aggccgatag caggctcaga
     5821 agacgctcca gcgtgaacat tgtgcgttca ccaaaaacgt gggccacgac agtcctgcgt
     5881 accctgtcat acgcgtaaaa cagccagtgc tggcgtgatt tagccccgac gtagctccac
     5941 tgttcgtcca tttccgcgca gacgatgacg tccctgcccg gctgtatgcg cgaggttacc
     6001 gactgcggcc tgagtttttt aaatggcgga aaatcgtgtt gaggccaacg ccaataaagc
     6061 gggcggttgc ccggcatccg acgccattca tggccatatc aatgattttc tggtgtgtac
     6121 cgggttgaga ggcggtgtaa gtgaactgta gctgccatgt tttacggcag tgagagcaga
     6181 gatagcgctg atgtccggca gtacttttac cgttacgcac aacgccgtca gtagcggaac
     6241 aggagggaca gctgatggaa atggaagcca ccggagcacc ttaaaagcac cgtcatccac
     6301 taaatcagta agttggaagc atcaccgcac tagcagacag ttaattaaaa aagtataatc
     6361 cctctccgga tttgttgaat aatggagaat tatgctgttt tctatgtctt atttagtcaa
     6421 atgcgcttat caatattgtg ataagctccc gagtctaaac ttaacttact gattttaggt
     6481 tgaggaaggc cggcattttt gtagatccac agcataatat ctatttattc aacaacacct
     6541 cccccaaaaa aaaaggtttt tataaatgtt tttaatttca attattcaaa tgttctcggt
     6601 tatgattata atgcttgata taaaaataaa agatgcattt agagcaatta ttctgctttt
     6661 acttctatta ctatccatgg ttatatctat tgaaataagc agcattatat caataggtga
     6721 gtacataccc acacttgcac tagcaaatat atcagagaca aatagtgttg gctggaaaat
     6781 aaaactacta ccttttcttg tgttcttgtc atttatgatt gcatcattga ttttgaaaag
     6841 attgataaaa g
//
LOCUS       DAJTRB010000163         6784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTRB010000163 DAJTRB010000000
VERSION     DAJTRB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.163"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            229..2661
                     /locus_tag="OO330_003959"
     CDS             229..2661
                     /locus_tag="OO330_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5884629.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2728..4197
                     /locus_tag="OO330_003960"
     CDS             2728..4197
                     /locus_tag="OO330_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5884630.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4197..5858
                     /locus_tag="OO330_003961"
     CDS             4197..5858
                     /locus_tag="OO330_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5884631.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFTLPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6079..6420
                     /gene="symE"
                     /locus_tag="OO330_003962"
     CDS             6079..6420
                     /gene="symE"
                     /locus_tag="OO330_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5884632.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6467..>6784)
                     /locus_tag="OO330_003963"
     CDS             complement(6467..>6784)
                     /locus_tag="OO330_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884633.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2004 a         1609 c         1551 g         1620 t
ORIGIN      
        1 aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgg ttcctgtaac
       61 ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa cagagatatc
      121 aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata gccaggctat
      181 tgattttatg tacaatcaga atcgtcctga taaaagcgag aaccccccat ggcggaactg
      241 aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc agccattctc
      301 aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg ggacggtaaa
      361 gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga catcgtgctg
      421 taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa acatgaaatt
      481 ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc ttttgttttt
      541 gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg tgaatgcctt
      601 gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca aaaattctgc
      661 ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta ttacgacgat
      721 ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac cattgaagcc
      781 gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg gaaaacctat
      841 accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg cattttgttc
      901 cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca gccatttggt
      961 acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat tcacctcgcg
     1021 ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca agtcgcacca
     1081 gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc cgaagacagc
     1141 gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt aaccgccacg
     1201 ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt ttacgtctac
     1261 tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt ccgtgttgat
     1321 attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt aaacggcgaa
     1381 gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt gatcgacgaa
     1441 cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa tccgatggat
     1501 aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg cgccctggtt
     1561 aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat caccggcgat
     1621 gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc gtacccggtt
     1681 atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg caaactggta
     1741 gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg tcgtggtaca
     1801 cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa aaaagccacc
     1861 gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga taccacacca
     1921 gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat cagcgaacat
     1981 gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca ggttaccgat
     2041 accgatgatg tcggcccatt tccggaagaa gacgagaaga aaatccgcaa gtttcacgtc
     2101 aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc cgacggtaaa
     2161 ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga atatgcctcg
     2221 ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgat cattcacgag
     2281 ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa agatctcgac
     2341 ccgttcgaca tgctttgcca cgtagtgtat ggtcagccgc cgttaacccg caaagagcgc
     2401 gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc gcaagccgtg
     2461 ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga aagtattcag
     2521 gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat taaaaccgga
     2581 tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga aatctaccaa
     2641 ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat catgttgcag
     2701 aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa atcattacaa
     2761 gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg tcagctctcc
     2821 tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact ggagcaggat
     2881 aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc caacgcgcag
     2941 ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc ggcgttgaaa
     3001 aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca ggcgttcagc
     3061 gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa caagctgaac
     3121 gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta cgaacagatc
     3181 cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg cgccgtcact
     3241 cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga cccggcctgc
     3301 ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata cgtgaagagc
     3361 gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa acagcttccg
     3421 cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt acaaattcgt
     3481 cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga tgtcattgtt
     3541 accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt tccggcagag
     3601 atgcaaaccc gcgaaacggc ggatctgttt ctgcaactga ttgtggaagt actggcgaaa
     3661 aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg tgttaaaacc
     3721 aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg tttaccgaat
     3781 ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac caaaggtcag
     3841 ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa aaactacagc
     3901 aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg gggtaacgag
     3961 gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag cattgatgac
     4021 gtgattgcgc gcaacttcaa tctggatatt aaaaacccac atcaggcgga aaccgtcagc
     4081 catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat ccagacgctg
     4141 cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaaggaggt taactaatga
     4201 gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg caaacccgtt
     4261 ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag aaattgcgtg
     4321 agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg aatgatgaac
     4381 cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg aagcaaggca
     4441 aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg tttgaattgc
     4501 cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct gagtcatcta
     4561 gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc gagcaaagtc
     4621 aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta aataatccag
     4681 ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat tttgttgtcg
     4741 gcgctgacgg tgtgaagatt ttatcaccta tattaatttg tgagcgtttc tttttttggc
     4801 agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa gtattaaata
     4861 gctgcttatt tacattaccc cccattgcag aacaagagcg aatagttgaa aaagttagct
     4921 ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg gacgcacatc
     4981 agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc gcggaactgg
     5041 ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc gaagccagcg
     5101 tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt gtgccgcagg
     5161 atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa aaagctcaat
     5221 tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc gatgaggaaa
     5281 aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata gttaatatta
     5341 aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca cctgatatta
     5401 ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca ggagtcaaag
     5461 ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc ccgtcattat
     5521 ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat ccattagact
     5581 gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt cttatgcaag
     5641 tcaaaaaagc agagaacaga ataaaaatgc caaaacttaa ttctgacagt ttttacaata
     5701 ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag ataaattcta
     5761 ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa acccaactgc
     5821 atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct cacaaaacca
     5881 ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc acgctaattt
     5941 tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat gatgtatcca
     6001 ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca caatcactat
     6061 ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt agaagcagaa
     6121 gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta cccggattac
     6181 agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg ttttgccacc
     6241 ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc ccaacccgcc
     6301 gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct gtcggcgcgt
     6361 aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa agtcgcctga
     6421 tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat accaacgcgc
     6481 tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga agttgtgcga
     6541 ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt gtcacatctt
     6601 tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac aatcgcagtc
     6661 ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca atatatagct
     6721 tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga agatgatcga
     6781 ataa
//
LOCUS       DAJTRB010000164         6558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTRB010000164 DAJTRB010000000
VERSION     DAJTRB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.164"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(226..429)
                     /locus_tag="OO330_003964"
     CDS             complement(226..429)
                     /locus_tag="OO330_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884634.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="OO330_003965"
     CDS             complement(431..586)
                     /locus_tag="OO330_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5884635.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="OO330_003966"
     CDS             complement(789..1196)
                     /locus_tag="OO330_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5884636.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="OO330_003967"
     CDS             1273..1500
                     /locus_tag="OO330_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5884637.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="OO330_003968"
     CDS             1484..1906
                     /locus_tag="OO330_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884638.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2773
                     /locus_tag="OO330_003969"
                     /pseudo
     CDS             1984..2773
                     /locus_tag="OO330_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2780..3526
                     /locus_tag="OO330_003970"
                     /pseudo
     CDS             2780..3526
                     /locus_tag="OO330_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3541..3963
                     /locus_tag="OO330_003971"
     CDS             3541..3963
                     /locus_tag="OO330_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5884639.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            4021..4377
                     /locus_tag="OO330_003972"
     CDS             4021..4377
                     /locus_tag="OO330_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884640.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4470..4688
                     /locus_tag="OO330_003973"
     CDS             4470..4688
                     /locus_tag="OO330_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5884641.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            4690..5055
                     /locus_tag="OO330_003974"
     CDS             4690..5055
                     /locus_tag="OO330_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5884642.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            5052..5717
                     /locus_tag="OO330_003975"
     CDS             5052..5717
                     /locus_tag="OO330_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884643.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            5717..6082
                     /locus_tag="OO330_003976"
     CDS             5717..6082
                     /locus_tag="OO330_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5884644.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1759 a         1394 c         1778 g         1627 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagaggaaga ggaagatcta aatactgatc tccccctaaa tccccctcgc
     2461 caaaaacgag cgtctaaaaa attcgagccg gaggctattg agctgcccga ttggttgccg
     2521 gaaacactct ggcatgagtg ggtccggttc agacaggcat tgcgaaaacc gattcgaacg
     2581 gagcagggcg atacgggaac tggaaaaatt ccgtcagcag ggttttacac ctgagcaggt
     2641 gattcgacac agcatcgcca atgaatacca gggcctgttc gcgccgaaag gtgttcggcc
     2701 tgagacgttg ctccgacagg ttaacaccgt ctcgttgccg gacagtgcga tcccgccagg
     2761 cttcaggggg taacggacca tgaaaaatat tgcgacaggc ggcgttctgg aacgcatccg
     2821 ccgactgacc ccgcaacatg taaccgcccc attcagaacg gtagcggagt ggcgcgagtg
     2881 gcaacttgct gaaggccaga aacgtagcga ggagatcaac cgcctgaatc gccagttgcg
     2941 ggtggaaaaa attctgaatc gctcaggcat ccagccgttg caccgtaaat gctcgtttgc
     3001 gaattaccag gtgcagaacg acggccagcg atacgcgtta agccaggcga aatccatcgc
     3061 cgatgaactg atgaccgggt gtacaaattt tgcgttcagc ggaaaacctg ataccgggta
     3121 gaatcactta gcggcagcta tcgggaatcg cctgctgaaa gacggtcaga cagtgattgt
     3181 ggttaccgtg gctgatgtta tgagcgccct gcacgccagc tatgacgacg ggcagtcagg
     3241 cgaaaaattt ttgcgggagc tgtgcgaagt ggatctgctg gttcttgatg aaattggcat
     3301 tcagcgcgag acgaaaaacg agcaggtggt gctgcaccag attgttgatc gccggacagc
     3361 gtcgatgcgc agcgtgggga tgctgacaaa cctgaactat gaggtgatga aaacattgct
     3421 cggcgagcgg gtgatggatc gcatggtcat gaaaggcggg cgctgggtta attttaactg
     3481 ggagagctgg cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt
     3541 atggcgaaac cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc
     3601 gtgcatcagg acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt
     3661 gcgtctgtcg gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc
     3721 tacggcttat tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc
     3781 tcgagagtaa aggtgaggaa accggttgtt gttgatccgg accttatctg gtcattacct
     3841 gacggagaaa tacgccgcta cgacaggcgc ctgaatataa tctgtcgcga gtgccggaag
     3901 agcgaagcta tgcagcgtgt actggctttc tatcagggta attttcagga ggcgatactg
     3961 tgaatgaaat tagctatcag gcttcaatta ccgctggcat tcgcatcaaa ggagaggagc
     4021 atggaaataa aaccagaaga tgagttaagc aatatcgttt tatttccgat aaaagaggat
     4081 gaccctcgta atcaggttaa ttttctttat aagccatcgg aaagaccata ttgccatcac
     4141 gcctctgtac gggttgacga aaaagagcgt caggtccgct gtaaaatctg cggtgcggtt
     4201 gtggagccgt ttgactggat gctctctgtg gcaaaaagag aaaccagact ggcagatgat
     4261 gtaaggctat tgcgccagga ggaacaggaa aggcggagaa atatagaaaa gctgatacag
     4321 attgagcgta acgcgaaagc gcggatacgc agggcgacaa aatccagaac tgaataatta
     4381 aatttagcac tgttaaaaat ttaatcctta accggaggga tttctgcacc ctcaaatcat
     4441 caggagaccg cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc
     4501 gaaagagtag aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga
     4561 tatccccgca aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt
     4621 ataacgacgt ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg
     4681 aaagctgata tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt
     4741 cgctgcgctt attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg
     4801 gtcattcgtt ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta
     4861 ccagaatgcc acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga
     4921 attatcagtg atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt
     4981 atgggattgg ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa
     5041 ggggctacgg catgactact attaccaaag agcgactgca atggctggct aacatttctg
     5101 gccgcgatga tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc
     5161 tggcatcact ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta
     5221 ttgacgggtt cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg
     5281 ctgccccgcg aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga
     5341 cgtttactca agttgaccag gaatctaata cgttcactgc tatatatttt tacaaagcgg
     5401 aagttgagcg ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc
     5461 tttacgcagc gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc
     5521 acgcactgtc agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc
     5581 gtgctgcctt tgccaacgtc actatccaac cagagccgat agtgccagat gaaatcgggc
     5641 caaacgatag taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc
     5701 ttaagggaga taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag
     5761 gcagcagaaa tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt
     5821 ggaaatggcc atgcgtaagg ctggcaactc tccggtaact ctggctggct ggataagctg
     5881 tagtgatgca gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt
     5941 attccccggt tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt
     6001 tgtggacaaa gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc
     6061 accgcaggag gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg
     6121 gcggtggcgc tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat
     6181 tgatggtccg gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt
     6241 taccatggca agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaat
     6301 tagcctggat agaaggtgct ggcaataaaa aatagcgttt tcttatcggt atcggtaaga
     6361 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ctgtaaggca gacagagaaa
     6421 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     6481 tgtctcacaa atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga
     6541 agcagcaaaa ggcgatgt
//
LOCUS       DAJTRB010000165         6511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTRB010000165 DAJTRB010000000
VERSION     DAJTRB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.165"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            429..1817
                     /gene="narU"
                     /locus_tag="OO330_003977"
                     /pseudo
     CDS             429..1817
                     /gene="narU"
                     /locus_tag="OO330_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            1899..5639
                     /gene="narZ"
                     /locus_tag="OO330_003978"
                     /pseudo
     CDS             1899..5639
                     /gene="narZ"
                     /locus_tag="OO330_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5636..>6511
                     /gene="narH"
                     /locus_tag="OO330_003979"
     CDS             5636..>6511
                     /gene="narH"
                     /locus_tag="OO330_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5884645.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1553 a         1713 c         1689 g         1556 t
ORIGIN      
        1 ataaacaata cttactataa taacgattat tacaatcagg caataagaga ggaaccgata
       61 gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat aaatcatcac
      121 taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc tttagattca
      181 cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt gaaggttagc
      241 ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg cccccgcggt
      301 tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt aattctaata
      361 actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt gtgcatatga
      421 ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc gactggaaac
      481 cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga aacctctgga
      541 tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc gcagttaccg
      601 ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta ttaaccgcat
      661 taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg cctatattcg
      721 gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc gtctggctcg
      781 gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc gctttgctat
      841 gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc ttctttccaa
      901 aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta ggtgtaagtg
      961 taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt ctcggcgtca
     1021 atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca tagatttggg
     1081 taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat atcgccagtt
     1141 cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc tggctgctga
     1201 gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt tttgccatgc
     1261 tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt ggcccattta
     1321 ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc ggcgtgcggg
     1381 tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc cttaccttac
     1441 cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg ctgtttctga
     1501 ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt cgccagataa
     1561 ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga gaagccgtca
     1621 ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc ggctttttta
     1681 ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc gcgatgaaag
     1741 tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat ggtcggcgga
     1801 agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat ccgacaaggc
     1861 atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt ttggatcgct
     1921 ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag gtgatgcata
     1981 gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa atcgtgcgtt
     2041 ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt aaaaatggtc
     2101 tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac ctgcccaatc
     2161 atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac agcgctaacc
     2221 gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa gccctcaaac
     2281 aacacagcga tccggtactg gcgtgggcat cgattatgaa cgatccgcaa aagtgcctga
     2341 gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag gaactaaacc
     2401 agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat cgcgttgccg
     2461 gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg cgttatctgt
     2521 cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg ccgcccgcct
     2581 cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg tataactcca
     2641 gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac gcccacttct
     2701 ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac tactctgaag
     2761 tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc gccctggcga
     2821 tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc gactacttta
     2881 tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag cctcgcgacg
     2941 atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat ggactgggcg
     3001 aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa ttggtagtgc
     3061 cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg gaatccattg
     3121 ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac gctgttgcag
     3181 gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc gtaaaacaca
     3241 atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat ggcaacacct
     3301 gtccggtggt cagtgtttat gatttggtac tggcgaatta cggcctcgat cgcgggctgg
     3361 aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca gcctggggtg
     3421 agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa tttgccgata
     3481 tcgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt aaccactggt
     3541 atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc ggttgtgtcg
     3601 ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc ccacaaaccg
     3661 gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag atgaacagca
     3721 cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct gctcaggagt
     3781 tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat ttcaacgttc
     3841 gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac ccgctcggga
     3901 ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc caggcgctga
     3961 aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat catccgcgta
     4021 atttgtttgt ctggcgctct aacctgcttg gctcctccgg caaaggccac gagtatatgc
     4081 agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt gccagcgacg
     4141 ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag ctcgacctgc
     4201 tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc gttctgccca
     4261 ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca tttattcatc
     4321 cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa atctacaaag
     4381 gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa accgacgtgg
     4441 tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt gaagtgctcg
     4501 actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat attgtggcgg
     4561 tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca ttgatggaca
     4621 aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc gatttcctcg
     4681 gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg attgacaccg
     4741 ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt catgttgcag
     4801 tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat ctggcgctgc
     4861 acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt aaaattatct
     4921 ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg ggatacacca
     4981 acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc tatcaggatc
     5041 atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct atcgacaccc
     5101 gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa gcacttaact
     5161 tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac ctgctaatgc
     5221 tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc cgagaactga
     5281 ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg actgcccgcg
     5341 cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc caggaacgca
     5401 ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat aactcggtca
     5461 cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg gcctggggct
     5521 ttaactacta cggcaccgtc ggctcgaacc gcgacgaatt catcatgatc cgcaagatga
     5581 agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa
     5641 aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct gccatacctg
     5701 ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg catggtttaa
     5761 caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc aggaagagtg
     5821 gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg gcagcaagat
     5881 gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg atgattacta
     5941 cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca aacatatccc
     6001 tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc
     6061 aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga
     6121 ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg
     6181 cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg gcgctatcta
     6241 caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt
     6301 gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga
     6361 aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt gctcagaaac
     6421 ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc gcatcgagga
     6481 agcgtcgagc accgagcgcg aagttgacct c
//
LOCUS       DAJTRB010000166         6462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTRB010000166 DAJTRB010000000
VERSION     DAJTRB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.166"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            459..1106
                     /gene="fucA"
                     /locus_tag="OO330_003980"
     CDS             459..1106
                     /gene="fucA"
                     /locus_tag="OO330_003980"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5884646.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            1134..2282
                     /gene="fucO"
                     /locus_tag="OO330_003981"
     CDS             1134..2282
                     /gene="fucO"
                     /locus_tag="OO330_003981"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5884647.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2335..3090)
                     /gene="xni"
                     /locus_tag="OO330_003982"
     CDS             complement(2335..3090)
                     /gene="xni"
                     /locus_tag="OO330_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5884648.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3201..4568)
                     /gene="sdaB"
                     /locus_tag="OO330_003983"
     CDS             complement(3201..4568)
                     /gene="sdaB"
                     /locus_tag="OO330_003983"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5884649.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4626..5915)
                     /gene="sdaC"
                     /locus_tag="OO330_003984"
     CDS             complement(4626..5915)
                     /gene="sdaC"
                     /locus_tag="OO330_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5884650.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPV
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1673 a         1551 c         1699 g         1539 t
ORIGIN      
        1 aatatttgcc tgtctgaaat gaactcatgg attcattttc aaataaaaaa cgctctgtaa
       61 tatgacggcg gtcacactta ttagctggaa ggtgacttta tgtgactacc atcactttaa
      121 taatcgaaat ttcatgcccg tttcagaaaa taaatcataa aaaacacaaa ccagatgacc
      181 gtattaattg tttgtgccga aaacgggcat ttctggcgag gggaaatgga aaaatgaccg
      241 ttttgataaa tatttttaag agcggtcatt tttaacgggg ttataaaaag agaacttgca
      301 gttatttact gtgattagtt gagctaggtc acaaaatata agcttgtgaa tttctttctt
      361 gttaatcagg aagaagatgg tgggtaatta aacggctaat tcaatagtgt gaaaggaaca
      421 acattattgc cctgttttga atcagagaga gatagctaat ggaacgaaat aaacttgctc
      481 gtcagattat tgacacttgc ctggaaatga cccgcctggg actgaaccag gggacagcgg
      541 ggaacgtcag tgtacgttat caggatggga tgctgattac gcctacaggc attccatatg
      601 aaaaactgac ggagtcgcat attgtcttta ttgatggcaa cggtaaacat gaggaaggaa
      661 agctcccctc aagcgaatgg cgtttccata tggcagccta tcaaagcaga ccggatgcca
      721 acgcggttgt tcacaatcat gccgttcatt gcacggcagt ttccattctt aaccgatcga
      781 tccccgctat tcactacatg attgcggcgg ctggcggtaa ttctattcct tgcgcgcctt
      841 atgcgacctt tggaacacgc gaactttctg aacatgttgc gctggctctc aaaaatcgta
      901 aggcaacttt gttacaacat catgggctta tcgcttgtga ggtgaatctg gaaaaagcgt
      961 tatggctggc gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc ctggcgatta
     1021 cggacccggt gccagtgctg agcgatgaag agattgccgt agtgctggag aaattcaaaa
     1081 cctatgggtt acggattgaa gagtaatttc gtaaagcaac aaggagaagg atgatggcta
     1141 acagaatgat tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg
     1201 atgaggtgaa acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc
     1261 aatgcggcgt ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga
     1321 tttacgacgg cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat
     1381 tccagaatag cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt
     1441 gtaaagcaat tggcattatc agcaacaacc cggagtttgc cgatgtgcgt agcctggaag
     1501 ggctttcccc gaccaataaa cccagtgtac cgattctggc aatccccacc acagcaggta
     1561 ctgcggcaga agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg
     1621 tttgcgttga tccgcatgat atcccgcagg tggcgtttat tgatgctgac atgatggatg
     1681 gtatgcctcc ggcgctgaaa gccgcgacgg gtgttgatgc gctcactcat gctattgagg
     1741 ggtatattac ccgtggcgcg tgggcactaa ccgatgcact gcacattaaa gcgattgaaa
     1801 tcattgctgg ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg
     1861 cgctcgggca gtatgttgcg ggtatgggct tctcgaatgt tgggttaggg ttggtgcatg
     1921 gtatggcgca tccactgggc gcgttttata acactccaca cggtgttgcg aacgccatcc
     1981 tgttaccgca tgtcatgcgc tataacgctg actttaccgg tgagaagtac cgcgatatcg
     2041 cgcgcgttat gggcgtgaaa gtggaaggta tgagcctgga aggggcgcgt aatgccgctg
     2101 ttgaagcggt gtttgccctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg
     2161 gtgtacgcaa ggaagacatt tcggcactgg ctcaggcggc actggatgat gtttgtaccg
     2221 gtggcaaccc gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt
     2281 aaatgcgctg atgtgataat gccggatacg atttgcggcg tatccggctc gccgttaccg
     2341 taccaaccgc aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat
     2401 atcgcggcac agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc
     2461 aaccgcatcc agattctcat atatcccttc cagactctga aactcgacca gcagctgcgt
     2521 ggcgcttttt ggtccgattc ccgcaacacc cggtaccttt gaactgctga tccccgcaag
     2581 tccccagtaa tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc
     2641 atccagccaa cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca
     2701 gtagccttta tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc
     2761 cagtgtggcg gctaagtcat cggcttcgtt gccgctggct gaccagcacg ggacgccgcg
     2821 ttgctcaaag gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg gcattggcgg
     2881 tcgatccgct ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc
     2941 atcaaaaacg gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcatg
     3001 ctggcaggtt tcgacacagg gcgaaccctg aacggcatga atgcgacgga taagattcag
     3061 tgcatcgaca ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg
     3121 gtaacattcg atgccggaac aggctatggc tggttgagaa aacaggaaga ggcttcgcaa
     3181 aacgaggcct ctgggagaga ttaatcgcag gcaacgatct tcatcgccag gccgccgcga
     3241 gaggtttcgc ggtacttggc gttcatgtct ttacctgttt cgtacatggt ttcgataact
     3301 ttatcgaggc agacgcgcgg ttcgctggta cggcgcagtg ccatacgcgc ggcgttcacc
     3361 gctttcactg ctgcaatggc gttacgctca atgcacggta cctgaacctg ccccgcgacc
     3421 gggtcacaag tcagaccgag gttgtgctcc atcgcgattt ccgctgcgat gcaaacctgc
     3481 gccgggcttg cgcctaacag ttctgccaga ccagctgccg ccatagagca cgccacgcca
     3541 acttcaccct ggcaacccac ttcggcacca gaaatcgacg cgttcatctt ataaagagaa
     3601 ccaatggcgc tggctaccag caggtaacga gccagtgagt tagcgttcac ttcgcggata
     3661 aacttatcgt agtacgccag cacagccggg ataatcccgc acgcaccgtt agtcggcgca
     3721 gtcaccacgc gaccgccagc agcgttctct tcgttcactg ccagtgcaaa catgttgatc
     3781 cagtcaacaa ccgccatcgg gtcagtggtg gttttatcct ggctgaccag catccggcgt
     3841 agtgccgcag cacggcgtgg aacgcgcagt ttgccaggca acacgccttc ggtggaaata
     3901 ccgcgctcaa taccgccacg catgacttcc cagacgttcg ccaggtgctg ttccagctct
     3961 tctttactgt gcagcgccag ctcgtttttc atcatcaggc cagagagtga cagcccggtt
     4021 tcctgacaat gtttttgcag atcggctgct gaactgtacg gataaggaac ttcaaccggt
     4081 gcgctatcct gctggccaaa atgctcttca tcaacgataa agccaccgcc aatagagtag
     4141 taagtctggc tgtaaacgac tttatcgccc gccagcgcgg taatgcgcat accgttttca
     4201 tgcagagaaa ggttgtctgc atggaagttc atgcactgat caaccgggaa ctccacttca
     4261 tgctgaccgt ttgccagcat caggcgacca tgagtattca catcctgaat aaaaccgggg
     4321 atggaatcga tatccacggt atccggcagg ttacccgcca ggcccataat aatggcgata
     4381 tctgtgtggt ggcctttacc agtcagagag agcgagccgt acacgtcaac caccacgcgg
     4441 gtcacgtctt taagcaggtt acgggcaatc agatcgtcgg taaattgttt acccgctttc
     4501 attggtccaa cggtatgaga actggaaggg ccaatgccga ttttgaaaat atcgaatacg
     4561 ctaatcatag gaaatacatc gcgttaaaac ggaggaagcg ccgcccgaaa gcggcgcgaa
     4621 aggacttagc tgaacagaga gtagaagatt gcggagattg caatcagacc catcacgaca
     4681 acgaatacgt tgctgatgtg accgctgtac ttacgcattg ccggtacttt ctgaattgcg
     4741 tacatcggca tcaggaacag gatcatcgcg atgattgggc cgcccagggt ttcaatcata
     4801 cccaggatgc tcgggttcag ggtggcaaca atccaggtcg ttaccagcat gaacagcgca
     4861 gtgatacggt tcagcttgtt gatttcgata gacttacctt taccacgcag agatttaatc
     4921 accataccgt tgaagccttc acgtgcgccc aggtagtggc cgaggaagga tttggtgata
     4981 gcgataatcg cgataatcgg agccatccac gcgataaccg gtgcgttaaa gtggttagcc
     5041 aggtaagaca gaatcgagat gttctgctct ttagccgcag ccaggtctac cggagtcagg
     5101 ctcagtacgc agctgaagac gaagaacatt acggtcagca ccatcatgat gtgtgcgaat
     5161 gccaggatct tcgagcattt ctgttctgcc atatcgccgt actcttcacg cttcgcaacg
     5221 gcgaaagaag agatgatcgg agagtggttg aacgagaaca ccattaccgg aattgccagc
     5281 cacagggtca tccacagacc gtttccggtt gcagatgcag tgtccagaga cagcgtttcc
     5341 agtgctgcgc cgttccactg cgggatcagg tacagagcca gcagcatcag tacgccaaca
     5401 aacgggaata ccagaatact catcgcttta acgatcatct gctcaccaaa gcgaacgatg
     5461 gtcatcatac ccacgatcag gatcagcgac agaatcgcac gcggcggtgg cgtcataccc
     5521 agctggtgag acatgaagct ttcaacggta ttggtgattg ccacgctata aaccagcagg
     5581 atcgggtaga tagcgaagaa gtagagcagg gtaatcagtt tacctgcgcc aataccaaag
     5641 tgttcttcta caacctcggt gatgtcttcg cccgggtttt taccagacag tacgaagcga
     5701 gtcaggccgc ggtgagcaaa aaacgtcatc gggaacgcaa ggatagccat gatgatcagc
     5761 gggatcatac cgccaacacc ggcgttgatt ggcaggaaca gcacgcccgc gccgattgcc
     5821 gtgccgtaaa ggcccagcat ccacatggtg tctgtcttgc gccaggcact acgagagtct
     5881 ttcgacgcaa tcgtgctggt ttgagtcgtt tccatctatt tctcctggag gaatgcaaat
     5941 aattcagctt ttagccagtt cagccaggga aggtgcctga ccgtaaaaat gaaatctgtc
     6001 tatcggcgat ggcttaatca tttaccgccg taataatttg agggcggaaa gatacacaca
     6061 agttatgtat ctatcagtga tcttgatccc aaatacaata atctgcttgg aagatagatt
     6121 atcccaacgc tttactctgt aaatcaaaag taaaagagaa tttaagggca atatttgccc
     6181 attaaacgac atctgacttc ttccggggcg cgcaatatat gtaggttgaa ctgaaaaaat
     6241 caatactttt agttaatcat ggcgataatt tctttttcat agcgataaaa tacggtttat
     6301 gaaaataggg aaacgtttgt gttgcaacat aaattgtatg acatattaac tgtgatgata
     6361 tttgtcgcgt gagggtggta agaaacattc atataacaaa tgaaatgagt tgatgagtaa
     6421 gtgaccaaaa acgagaaaac ccggcaacaa acgtcaccgg gg
//
LOCUS       DAJTRB010000167         6407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTRB010000167 DAJTRB010000000
VERSION     DAJTRB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.167"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO330_003985"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO330_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5884651.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO330_003986"
     CDS             complement(1433..1759)
                     /locus_tag="OO330_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5884652.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO330_003987"
     CDS             1894..2235
                     /locus_tag="OO330_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5884653.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO330_003988"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO330_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO330_003989"
     CDS             complement(2833..3543)
                     /locus_tag="OO330_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5884654.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO330_003990"
     CDS             3651..3956
                     /locus_tag="OO330_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5884655.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="OO330_003991"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="OO330_003991"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="OO330_003992"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="OO330_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1708 a         1628 c         1500 g         1571 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatgttata ttattatatt
     6001 aacaacaggt ccagaccatt ggtccagacc atagttagtt aatcacagtg atagcccacc
     6061 acctgaagtc ctgctgatat tcagtccaga cttacctctg ctcatggtct tacctccctc
     6121 ggtctcctga ccttgggtat cgactacagc ttgctgagcg tcatctggag gagctacagg
     6181 aggcgggacc gaagccccac cttgagtccc accaccacct gtcaggccct tggcgatgga
     6241 cctgaagcct ttactaatgc tcttacccat tggtcttacg cttcggtctt ggtgaacttc
     6301 aggcgtttga ctacaggcag gacggcagta gctgagccag tgcccatctt gacggtcgta
     6361 gcagcttcag cgacaacgaa cactttaccg aggatttcca agtcgtg
//
LOCUS       DAJTRB010000168         6321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTRB010000168 DAJTRB010000000
VERSION     DAJTRB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.168"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..378
                     /locus_tag="OO330_003993"
     CDS             <1..378
                     /locus_tag="OO330_003993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884656.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OO330_003994"
     CDS             complement(454..918)
                     /locus_tag="OO330_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5884657.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO330_003995"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO330_003995"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5884658.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="OO330_003996"
     CDS             complement(1787..2476)
                     /locus_tag="OO330_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5884659.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO330_003997"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO330_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5884660.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO330_003998"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO330_003998"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5884661.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO330_003999"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO330_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5884662.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1609 a         1709 c         1565 g         1438 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAJTRB010000169         6281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTRB010000169 DAJTRB010000000
VERSION     DAJTRB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.169"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..1171
                     /gene="pgaC"
                     /locus_tag="OO330_004000"
                     /pseudo
     CDS             1..1171
                     /gene="pgaC"
                     /locus_tag="OO330_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1173..1586
                     /gene="pgaD"
                     /locus_tag="OO330_004001"
     CDS             1173..1586
                     /gene="pgaD"
                     /locus_tag="OO330_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HCR5884663.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(1636..2424)
                     /gene="phoH"
                     /locus_tag="OO330_004002"
     CDS             complement(1636..2424)
                     /gene="phoH"
                     /locus_tag="OO330_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5884664.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3032..4297)
                     /gene="efeB"
                     /locus_tag="OO330_004003"
     CDS             complement(3032..4297)
                     /gene="efeB"
                     /locus_tag="OO330_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5884665.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4303..5430)
                     /gene="efeO"
                     /locus_tag="OO330_004004"
     CDS             complement(4303..5430)
                     /gene="efeO"
                     /locus_tag="OO330_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5884666.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5488..6162)
                     /locus_tag="OO330_004005"
                     /pseudo
     CDS             complement(5488..6162)
                     /locus_tag="OO330_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
BASE COUNT         1515 a         1666 c         1523 g         1577 t
ORIGIN      
        1 tgggtctgtc gtgaacgcca ctggccgtgg ggagaaaacg caccagctcc ccagttgaaa
       61 gataatccgt ctatctccat tatcattccc tgttttaatg aggagaaaaa cgttgaggaa
      121 accatacacg ccgctttagc acagcgttat gagaacattg aagttattgc cgtaaatgac
      181 ggttcaacag ataaaacccg tgccatcctg gatcgcatgg ctgcacaaat tccccatttg
      241 cgggtcattc atctggcgca aaaacagggg aaagccattg cgcttaaaac cggagctgcc
      301 gcggcgaaaa gtgaatatct ggtgtgcatt gatggcgatg cgttattaga ccgcgatgcg
      361 gcggcatata ttgtggaacc gatgttgtac aacccgcgtg tgggtgccgt aaccggtaat
      421 cctcgtattc gaacacgttc taccctggtg ggtaaaattc aggttggcga gtattcctca
      481 attattggtt tgatcaagcg aacccagcgt atctatggaa acgtatttac cgtttccggt
      541 gttattgccg catttcgtcg cagcgccctg gcagaagtgg gttactggag tgacgatatg
      601 atcaccgaag atattgatat tagctggaag ctgcagttga atcagtggac gattttttta
      661 cgagccacgg gcgctgtgct ggatattaat gcctgaaacg ttaaaagggc tgtggaaaca
      721 gcgcctgcgc tgggctcagg gcggtgcaga agtattcctc aaaaatatga caaggttgtg
      781 gcgcaaagaa aactttcgaa tgtggccgct gttttttgaa tactgcctga cgacaatatg
      841 ggccttcacc tgcctggtcg gtttcattat ttacgcagtc caacttgccg gtgtaccgtt
      901 aaatattgaa ttgacacata tcgctgcgac acatactgcc ggaatattat tgtgtacgtt
      961 atgtttactg caatttattg tcagcctgat gatcgagaat cgctatgagc ataatctgac
     1021 ttcatcgctt ttctggatta tttggttccc ggttattttc tggatgctga gcctggcaac
     1081 gacattggta tcatttacac gagtcatgtt gatgcctaaa aagcaacgcg cccgttgggt
     1141 aagtcctgat cgcgggattc tgagaggtta atatgaacaa tttaattatt acgacccgac
     1201 aatcaccagt acgtttactg gttgattatg ttgccacaac catcttgtgg acattatttg
     1261 ctttgttcat attcttattc gccatggatc tgctgacggg ttattactgg caaagcgagg
     1321 ccagaagccg acttcagttc tattttttgc tggcagtggc gaatgccgtc gtgttaattg
     1381 tctgggcgct gtacaataag ctgcgttttc aaaaacagca gcatcatgca gcctaccaat
     1441 atacgccgca agaatatgca gagagcttag caatacctga tgagctctat cagcaactac
     1501 aaaaaagcca caggatgagc gtacacttca ccagccaggg gcaaataaaa atggttgttt
     1561 cagaaaaagc gctagtccgg gcataaacac ccaaaacaaa gcccggttcg cccgggctct
     1621 gcaccgataa tacacttaac tgtatgcatg cagcgtacgt tggcaaagtg ccgaacgtac
     1681 gcaatcctct tttccgaacc ggacgatccc aaccatttca tcttcttcga aacgttccag
     1741 cgcgtcactt aatccggagc acacgccgcg aggcaaatcg cattgcgtga tatcaccgtt
     1801 gacgataacc gtcacgttct ccccgaggcg ggttaaaaac attttcattt gcgcggcagt
     1861 cacattctgc gcctcgtcaa gaatgacaac tgcattttca aaggtacgtc cacgcatata
     1921 ggcgaacggc gcggtttcca ccttccctat ttccggtcgc aggcagtact gcataaagga
     1981 agcccctaag cgccggacca gcaagtcgta gaccgggcga aaatagggag caaacttttc
     2041 tgcgatatca ccaggtaaga agccaagatc ttcatcggct tgcagaactg gacgggtgac
     2101 gataatcctg tcgacatcct tatgtatcag ggcctctgcc gcttttgctg cgctgatcca
     2161 ggtttttccg cacccggctt cgcccgtggc gaatatcagc tgcttactct caatagcctg
     2221 cagatagtgc aattgcgctt catttcgcgc aaggatgggc gaggtatcgc gactgtcgcg
     2281 ggccatacca atggcttcta cgccgctcat ctgcacaagc gaggtgaccg attcttcttc
     2341 acgctgctta tggctgcgcg aatcccgtct cagcacacgt tttgcctcgc gacgagcttt
     2401 gatcactgct ttttgtcttc ccatggagag caccttgagt tgtttgtatt catcacacgc
     2461 gccgttggca gcgcgattat gcgcacgaac atcagagggt tggcttcctt gtaagccata
     2521 gtttgctttt ggataaaatg ccgaaaaacg gctacgcaca ccgtttgcgg cgtcggtaac
     2581 acgagtaaag aaaggaagtg agaaaaatac agagtgacga agttgctacc ggaggagaaa
     2641 tctccgcgag tggtggagcg ttgcttaaca aaacattgtc cagaacagga cgttaccatc
     2701 cgcgatctcc atagtgactg actatcactg ccgggaactt ccgctgctac ttaataagta
     2761 caacagaact cgcattcatt gcaacaatat attcacttat atttaacgat aaacacccat
     2821 ttcggtgaca ttaatttcta ctggaaagat gacagagtga tgacagtgat ggaaaaagct
     2881 gtgtgctttc cgcaggattc gcagggctgg taaaatgtgc ctcgtgtttg ccggattcgg
     2941 cgcgaacgcc ttacccgacc tacaagtgat taataatttg tgcacaaaac tcatgcccga
     3001 taaacctgcg catcgggcat atttgcgtcg ttcaaacctg caatagcgcc cttcccagat
     3061 aatcgttcgc gtccttcact cccggcagcg aaaaaaaata accgccgccg ataggtttaa
     3121 cgtattcctc cagcgcttcg ccattgagcc ttttttgtac tgtcaggaag cctttttcca
     3181 gatcgtgttg gtagcagaca aacagcaacc ccatatccag ttgtccggag ttggtgaccc
     3241 ccaacgaata actgtagccg cgacgcagca tcaggctgga ctcactctcc gccgtgcggg
     3301 gattcgccag ccggatatgg ctgtccagcg cgatcacctt cccttccggg tcgctggcgt
     3361 aatcaggcac atcatgctca tgctgcattc ccagcggcgc accggtttgc ttatcacggc
     3421 caaaaatcgt ctgctgttct ttcagcggcg ttctgtccca aaattccact cgaaactgaa
     3481 tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc aggctcctgc tgatctgccg
     3541 ttacccacac cactttttgc atcaacttat cattctggct atcgggattg gcagtgccgt
     3601 ctttgaaacc cagcaaatta atcggcgtct ctttgccttt actacgcgcc gcgtgatcgg
     3661 aaataaaccc ttcccgcttc cagcgcacac tgagcaaatc cggcgtgtgt ttgatgatat
     3721 cgcgcagcgc atggttcacc gtgtcctggg tgttggcgca aatctgtagc aacacatcac
     3781 catgacataa cgccgcatcc agcgagtcgt tggggaaacg cgtcatcttc tgcagctttt
     3841 ttggcatctg tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc accgataacg
     3901 tgatggtgag attatcgggc gcaatgtagc cgccaagaat gccggaatcg agtggtggca
     3961 ggcgcggatt tggcgtttct ggtgctgctc cgccctgact cagaaaagca aaacgctgag
     4021 tcaacaagcg aaacaaccgc tcaagatcgg ctttatcgct ggcaagcaca tcaaacgcca
     4081 ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc ctgatgctca ccataaaacg
     4141 gctgtttctc attgcgtgca tccggtgaaa gcgtgcccgg tgcactttgc gttttttgtg
     4201 catgagcgac cggacaactt cccgccagcg caccaatccc tttcagtaaa cgtcggcgtg
     4261 acggttcatt cacgccgttt tcatctttat actgcataac gcttaatcca gtcccagcac
     4321 accgcgaagt tgcgccagat cttccgccag cgcagtaatc ggccctttca gcgcattccg
     4381 gtcggcatcg gtcaatttgt cgtaggtttc aaaaccgtct ttagtacggt atttcgccag
     4441 aatggtatcg acctttttaa agttggcatc gacttttgcc aacagttccg ggttggcttt
     4501 ttgcagttgt ggacgcagca gatcgacaat tttctgcgag ccttcaacgt tagcctggaa
     4561 atcccacaga tcggtgtggc tgtagcgatc ttcttcaccg ctgattttgc tggctgccac
     4621 ttcctcaatc agtccggctg cgccgccgac cacttttgaa ggtgggaaag ccagttcact
     4681 gatgcgtttt tgcaaatcga ccacatcggt ataaagctgc tcagcgtact gatccatccc
     4741 tttggtggtg ttgtcgccaa acaatgcttt ttccagacgg tggaaaccag tgaattttgg
     4801 gtcggcggct ttttgctcgt aatcatcttc acgggcgtca atgctgccat ccagatcgga
     4861 gaacagttca gcaatcggtt caatacgctc atagtgctgg cgcgtcggcg catacagtgc
     4921 tttcgctttt tcgatatcgc ctgctttaat cgcgtcggta aaggctttgg tgtcggtcac
     4981 cagctgcgtg gtttccgcca tgacatacgc tttatatgca gtaattgcac caccaagact
     5041 taacagcgca tcactttgcg ccgcatccgc cgttgcctca cctttgacga tcaacttccc
     5101 tttcgggtta gtcagcagac cgcaggtcat atcgtattcg ccaggctgta aattcgccgt
     5161 cattttctgg ctaaaaccag gggcgatatt ttcccgctct tccaccacca tcacgccttt
     5221 gaggatctcc cactccagcg ccttctggct atggttctga ataatgaact gtgttttccc
     5281 ggcgttaacc gtaatggtca tcggttcgca ctgcttatcc gtcacggtca ctttgacctg
     5341 cggcacatca gcggcgttag ccataaaagc agaagaaaac agcgcagcca cgctcaactg
     5401 caatgcgtta cggcggaagt taatggtcat gacgagacta tccctttaaa gtatgttgta
     5461 actaagcaag agtttgcgtc gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg
     5521 tggtggcaga gcaaatgcca ccagcgccgg gatgagataa ataaaccaga cggcgacttc
     5581 gctgacgctc ggtgcttcct gatagccaaa aatcccttcc atcagcgtgc caaacagcga
     5641 gtgagttgag aggaccgcgc tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc
     5701 ggcttcatga aatgcgcgaa tggcaccagc cgccagccct gcggcgacga agagaataaa
     5761 caggctggtc catttaaaaa atgcaccaag attgaggcga ataccgcccc agtagagcag
     5821 gaagccgagc accacggcag tagcaagacc gagcattgca cccagcggcg gccagatccc
     5881 gacatcttgt tgaaatgccg ccagcaggaa aaagaccgac tccagccctt cccttgcaac
     5941 ggcaaaaaag accatcatca ccagcgccca gccatgatga tttccacgct gcaatgcgct
     6001 atcgactgcc tgttccagtt gcactttgac gttgcgcgac actttgcgca tccagaaaac
     6061 catccaggta aggatcacca cggcgatcac cgccacgata ccttcaaaca gttcctgctc
     6121 tttttgcgga aattcgccgg tggtttcgtt aatgaagatc cccgagtagg cagcctgacg
     6181 gctgcggctt gtcatggtct gcaattaccg ttataaaaac aggcaatgtc attgtctttc
     6241 aggtggttat atggcccgtt cagctaaacc ccgtaaacgt a
//
LOCUS       DAJTRB010000170         6255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTRB010000170 DAJTRB010000000
VERSION     DAJTRB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.170"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..653)
                     /locus_tag="OO330_004006"
     CDS             complement(<1..653)
                     /locus_tag="OO330_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCR5884667.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRI"
     gene            complement(643..2400)
                     /locus_tag="OO330_004007"
     CDS             complement(643..2400)
                     /locus_tag="OO330_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5884668.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFYKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2416..3300)
                     /locus_tag="OO330_004008"
     CDS             complement(2416..3300)
                     /locus_tag="OO330_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5884669.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3311..3916)
                     /locus_tag="OO330_004009"
     CDS             complement(3311..3916)
                     /locus_tag="OO330_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5884670.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(4087..>6255)
                     /locus_tag="OO330_004010"
     CDS             complement(4087..>6255)
                     /locus_tag="OO330_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5884671.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1833 a         1565 c         1313 g         1544 t
ORIGIN      
        1 ttgattcgac gttgatcaag tttctgataa ttccaacgac ggtcgacggg caccatccag
       61 tcaatcaaca tacctaccgc cagccctgtg atgacatcaa taaaatgatg ctgccatgtc
      121 gtcagcgtcg aaatggcgat gagtaaaaac catccgccac agactttacg ccacctcaca
      181 gccagatgct gacgaaagtg acgccaaagt agccagcaga gaataatatg cagcgaagga
      241 gactggttat aaggcagatc aaacagttca agttgcgaaa atagccatcc tgtcacccca
      301 ctcacttcag gacggataaa actaaacttc agcgggtaga gaaaaaaacc gcagcaggcc
      361 attaccgttg ccagaataag ccggtggaca agtcggcgtt gttcgaatgt cgagctacaa
      421 acgaacagcg aaaatccata taaaagatcc agactccagt aaggaacaat agtccaggga
      481 agaaaaggga tcgccgtttc ccaaccgaat acctgactgg ggatatcatg gctgttaagg
      541 tcctgaaccg cggtgaactg attaagagat ccataggtga agaagaaaaa cggggccagc
      601 aacaataacc agccagcgcc ttgtagcaat acgttccgac gttcagttat cacgacgcac
      661 cgccatcgaa accgtaaaaa tgccccacac atcaatccgt tgagtgcatt tataaaatcc
      721 ggcattacgc acgagcgaat ccatctcccc ttgcgaacgc acgcgcatta cccacggttt
      781 accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt gccacggctg
      841 cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg cgagtgagtt
      901 ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg ccagcgtagg
      961 ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt caaaacgcac
     1021 ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat ttaactcact
     1081 gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat ccagtacata
     1141 gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg cgtggagatc
     1201 ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa taccgcgcca
     1261 tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac tgccttgcgg
     1321 ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac tcattccgag
     1381 atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa agcgatacgc
     1441 taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc gatccgctga
     1501 tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag catataagcg
     1561 gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac ccaatgtgtc
     1621 gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc tctgataaaa
     1681 atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa gttgcgtggg
     1741 gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga gatcgagcaa
     1801 gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg ccacccgttg
     1861 ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata gaccacgcag
     1921 acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa ccttaaaggc
     1981 cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc atgtggcaac
     2041 aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga cctggctgtc
     2101 gctgacagca aaacggacaa attcatcgac atcctgcact gagcgcataa gagacgggct
     2161 ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat aaaaagcagt
     2221 atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt catgcccgcg
     2281 atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt gccgataata
     2341 caacgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg aattttccat
     2401 tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata ccggagcggt
     2461 gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata gttttccact
     2521 atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc aaaaaccact
     2581 aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg tcagcaacgg
     2641 tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac cttccagcgt
     2701 ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc cccataaata
     2761 ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta acgccccgcc
     2821 ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg caaaaacggt
     2881 cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg ggtcacccac
     2941 aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata acagaaacag
     3001 ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat ataaccaacg
     3061 cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac ttatcagcgt
     3121 aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac aaatgacgat
     3181 gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag gtaaccgcca
     3241 tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt tgccagagat
     3301 ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga ttaatcgcag
     3361 tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc atccactgtg
     3421 gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca cttttgccaa
     3481 atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg caaaactcgg
     3541 tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc ggtaaaaata
     3601 aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc aaaatcgccc
     3661 ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca ttgagcgcca
     3721 tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc accaacacca
     3781 acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga ttcgctgtaa
     3841 cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag agcggtttta
     3901 tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt gcatcaattc
     3961 ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata tcaaggtatt
     4021 gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa attattacgc
     4081 tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg cgcgcgtctt
     4141 accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct ctaacgccag
     4201 caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg gattattcat
     4261 cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg gggtgtggcg
     4321 gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg tactccacca
     4381 tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg ggtcgagtgc
     4441 ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct gcctcagaaa
     4501 aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga gaacaacagc
     4561 gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt ccaaacgtat
     4621 cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa tcagctcaga
     4681 caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat tatttttccg
     4741 atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat actgattttt
     4801 cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt gtaaagcagc
     4861 gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt ttttctccat
     4921 ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca cacgtattag
     4981 atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac tctcttgtgg
     5041 cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg ctaagcttgt
     5101 gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag tgagttgtcc
     5161 tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat caataatgga
     5221 ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc aatctctttt
     5281 cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat tcaacggacc
     5341 gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg gttcacggtc
     5401 gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact ccagaatctc
     5461 attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc tattaataag
     5521 ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca ttatggcaag
     5581 atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat ccagcatttg
     5641 caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt ttttcatctg
     5701 attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt tcagagcatc
     5761 ttcatcattt attagacccc aataatatgc atcaggattt attatcatca gcgctttttt
     5821 gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat ttgtcgaaga
     5881 cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa tactcccctt
     5941 aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg catttatcga
     6001 accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt taccgagaat
     6061 aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca aattgccaag
     6121 aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc cacgatgcac
     6181 tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt tgatagcttg
     6241 ttcaacttgt tgttt
//
LOCUS       DAJTRB010000171         6238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTRB010000171 DAJTRB010000000
VERSION     DAJTRB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.171"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            360..1184
                     /gene="gadX"
                     /locus_tag="OO330_004011"
     CDS             360..1184
                     /gene="gadX"
                     /locus_tag="OO330_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5884672.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1552..2280
                     /gene="gadW"
                     /locus_tag="OO330_004012"
     CDS             1552..2280
                     /gene="gadW"
                     /locus_tag="OO330_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5884673.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2643..5756)
                     /gene="mdtF"
                     /locus_tag="OO330_004013"
                     /pseudo
     CDS             complement(2643..5756)
                     /gene="mdtF"
                     /locus_tag="OO330_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5781..6038)
                     /locus_tag="OO330_004014"
                     /pseudo
     CDS             complement(5781..6038)
                     /locus_tag="OO330_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1651 a         1452 c         1475 g         1660 t
ORIGIN      
        1 ttgtaaaaac cgaatgccca gcctttaaaa aaacagctgg gcattcggtt gcttattaat
       61 cgcaataata tattggctgt ctattcatcg tgttgataag atatatgtta tatgaatgtt
      121 tattaaatgt agcgattacc tttaatgtca cgaaaaggga ttatttgctt gctattaatt
      181 tcactgcgtt cttaaacacg tttttgtatt ataaatccag tcatcctgcc cgtagaacat
      241 cacacattat catcctgttc tcccgcttcg tttaaattta tttatcaatc aatttgactt
      301 aagagggcgg cgtgctacat taattaacag taatatgttt atgtaatatt aagtcaacta
      361 tgcaatcatt acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca
      421 tggttaatgg tgaatatcgc tattttaatg gcggtgacct ggtttttgcg gatgcaagcc
      481 aaattcgagt agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat
      541 tatttctgcc gatgctcaag gaggaggcat taaatcttca tgcacataaa aaaatttctt
      601 cattactcgt tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat
      661 cgcagaataa gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt
      721 tgttatctgt ttttcttgag gatgagcact ttataccgct gcttttgaac gttttgcaac
      781 cgaacatgcg aacacgagtt tgtacggtta tcaataataa tatcgcccat gagtggacac
      841 tagcccgaat cgccagcgag ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg
      901 aagaagagac atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac
      961 ttattgttat acatggcttt tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg
     1021 tgtcgtattt catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg
     1081 agcgatcggc gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggga
     1141 atttttacgg cactaaccgt tctgcggaag gaataagatt atagagtttt actcagacat
     1201 aaaaaaaacc cggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa
     1261 ccgttataac actccctgtt ggcacgggaa actttgtgct ctcagtaagt taaatataac
     1321 ttttactgga aataagatca gccatttttt tataaacata agctatacgc tgtgcgaaaa
     1381 tatattcgtg ctgcatttac ttattatcaa ttaactgtta tgcaaaacta ctttgtggat
     1441 aaattttggt cctaccaaat ctggcagttt ttgcgctaag aaacagtctg tcatcatttc
     1501 attagtatac tgaaattgaa ataatcgcag tatgaaatat aagggataat catgactcat
     1561 gtctgctcgg tgatcctcat tcgtcgttca ttcgatattt atcatgaaca gcaaaaaata
     1621 tcgctgcata acgagagtat cctgctgctg gagaaaaatt tggcagacga ttttgcgttt
     1681 tgttcaccgg atacgcgacg actggatatc gatgagctga cagtttgcca ttacttacaa
     1741 aatattcgtc agctaccacg caatttaggg ttacatagca aagaccgtct gttaattaac
     1801 cagtcacccc ccatgccgct ggtgacggcg atttttgata gcttcaatga atccggggta
     1861 aattcaccga tactgagcaa tatgctctac ctttcctgtt tatcgatgtt ttctcataag
     1921 aaagaactga tccccttact tttcaatagc atcagcactg tttcaggaaa agttgaacgc
     1981 cttattagct ttgatatcgc caaacgttgg tatctgcgcg atatcgcgga aagaatgtat
     2041 accagcgaga gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata
     2101 ttactcgctt ccaggatgtc gatggccaga cgattactcg agttacgtca aattcctctg
     2161 catactattg cggaaaaatg tggctatagc agtacgtcgt actttataaa cacatttcga
     2221 caatattatg gtgtaacgcc acatcagttt gcgcaacatt cgccaggtac cttttcctga
     2281 catattttgc atttgaatat tggtcaggat ctcacacctg cttcatgtga aactcttccc
     2341 tgatgatttc tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt
     2401 atttttagga ggaaaagact cggcatgttt gggattatta agctgacaat tcatacgatt
     2461 acgggaatgt gggtcagtat tgtgttgttc aaattgatga cgaatggctg gagtggtttc
     2521 tactttcagt gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt
     2581 gaatggttag caggaaagag taaggctgaa ccttcacgtt caaccttact ctcatttaca
     2641 cgttacgctt ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg
     2701 aagtaaattg ccagcactgt tgcggcaaac atcccgccca tcacgccggt acctaccgcg
     2761 ttttgcgcgc cagaaccggc accatgactg ataaccagcg gcagcacgcc gagaataaag
     2821 gccagagagg tcatcaggat tgggcgtaaa cgcatccgcg ccgcttcgat gattgcctct
     2881 atcggcgttt tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg
     2941 ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct ggaagtagac gtcattactt
     3001 aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc
     3061 accgagaacg gaattgacca gctctcatag agtgcggcga gggcgaggaa caccacgacc
     3121 agtgaaatgg catacagtgc aggagcctgg tttgaggata acgcttcctg atacgacagt
     3181 ccggtccatg agtagccgac gcctgccgga agtttagcga ccaggtctgc cataaatttc
     3241 atagcgtcac cggtactttt cccggccgcc gcttcaccta aaatctccat tgacgggatg
     3301 ccgttgtagc gttccaatcg cggtgaacca taggtccatt cggtagacga gtaggcagaa
     3361 agcggtgcca tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac
     3421 atacggaacg gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag
     3481 tcgttcacgt agctgctgcc gaaggaggtg gaaattgtct ggttgatatc agacagcgcc
     3541 acacccattg cctcagcttt cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc
     3601 aggccgttcg ggcgtacccc ggtgacctgg tcaggcgatt gcgctgccag tgataacagc
     3661 tcgtttcgtg cctaggttag tttttcgtgc cccaggttac cgttgtccag cagttccata
     3721 tcaaaacctg acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg
     3781 gctttattga tactgcttaa cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt
     3841 tcctcaccga cacgttcaga ccacggcttg agactgataa acgccaggcc gttgttttgc
     3901 ccctggccgc tgaagccaaa gccgccaacg gtaaacaccg actgaacatt atctttctct
     3961 tttgtaagat aataatccgt cacctgttgc agtactttcg tggtgttaac catggtggca
     4021 ccggaaggta actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag
     4081 gtcggtgtac gcaggaacag caccgccatc ccggcgcaaa tcagcaggta gaccaccatg
     4141 tagcgaccgg tacaacgcaa cagcgagcgg gtgctatcgg tatagtgctg agttgatttt
     4201 tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt tgtgaccgcc ttccggcgtg
     4261 gctttcagaa tggtggcgca cagggcaggg gtcaggctca ttgccacaaa tactgaaagc
     4321 cgcatggagg agatcagcgt gatggagaac tggcggtaga tctccccggt tgcaccgctc
     4381 ataaaggcca tcggcataaa cactgcagaa agaacaacgg caatcccgac cagcgcacgt
     4441 tggatctgcc ccatcgattt atgcgtcgct tccttcggcg gtagcttatc ttccgcaatg
     4501 acacgctcga cgttctccac caccacgatg gcgtcatcca ccagtaaccc tatcgccagc
     4561 accatcccga acatagtcaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac
     4621 gtcccgagaa taaccaccgg tacggcaatt gtcgggatga tggtggcacg gaaattctgc
     4681 aaaaacagat aaatgaccag gaagacgagg atgatagcct caaccagtgt tttgaaaact
     4741 tcctgaatag aaatttcgat aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt
     4801 gcggggaaat aggctgataa gcggttcagc tcctctttga ctgcccgcga ggtatccagg
     4861 gcgtttgctc cggcagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc
     4921 gccacggtgg aataatcttc cgccccaagt tcgacgcggg cgacatcacg cagcagcact
     4981 tgcgaaccat cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga
     5041 cgcgtctgca caatgatcga ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc
     5101 agttgaccac cagaaatctg gttgttttgc accttaatct gggaaataac atcggaaggt
     5161 accaggttat atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa
     5221 ccaaagagct gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac
     5281 gctacatagt ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaacgccgct
     5341 accatcagga tattactgct cgacttatcg acgctaatcc cctgttgctg caccgcttca
     5401 ggtaatgaag gcatagcgag ttgcagttta ttttgcacct gaacctgtgc gatatcagga
     5461 gatgtcccag tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa
     5521 gacatgtaca tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag
     5581 tcttctaccg tttgcgcatc ggcacccgga taggtagcgc tgacggtaat ggtcggtggc
     5641 gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc
     5701 ataataatgg caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa
     5761 gtccccttaa gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag
     5821 gaaattgctc gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga
     5881 tcgccagcct gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca
     5941 atttcgcgta gctgcacgac atcgtcttta tccagaatga gcgccgttgc tttaccctgg
     6001 gcgttgtggg tgacgccttc ctgcggcacc agtaatataa agcgcgcaaa attattcaag
     6061 atatttttga aaatcattca agataggcta tcgcgtgtga tagcaaaaac attcaagata
     6121 aaaatgcttg attattaagc aatcaggaat gggagtatta agtttcgtgt ttccagtaaa
     6181 accagagagt attgaaatag ctatcaccta actttatcag gcgatagctg gtagatga
//
LOCUS       DAJTRB010000172         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTRB010000172 DAJTRB010000000
VERSION     DAJTRB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.172"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO330_004015"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO330_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5884674.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO330_004016"
     CDS             2089..2238
                     /locus_tag="OO330_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884675.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO330_004017"
     CDS             complement(2983..3423)
                     /locus_tag="OO330_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5884676.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO330_004018"
     CDS             3641..4030
                     /locus_tag="OO330_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884677.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO330_004019"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO330_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OO330_004020"
     CDS             complement(4585..5421)
                     /locus_tag="OO330_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5884678.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OO330_004021"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO330_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5884679.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1432 a         1617 c         1642 g         1509 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTRB010000173         6091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTRB010000173 DAJTRB010000000
VERSION     DAJTRB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.173"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..534
                     /locus_tag="OO330_004022"
     CDS             <1..534
                     /locus_tag="OO330_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5884680.1"
                     /translation="ASSFFGAKFQRNIEELLVAPVPTHVIIAGYVGGGVARGLFVGIL
                     VTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLNGVFAKTFDDISLVPTFVLTPL
                     TYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGFLGINDVPLVTTFGVLVVFIVA
                     FYLICWSLIQRGRGLRS"
     gene            639..1079
                     /locus_tag="OO330_004023"
     CDS             639..1079
                     /locus_tag="OO330_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5884681.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            1143..2372
                     /locus_tag="OO330_004024"
     CDS             1143..2372
                     /locus_tag="OO330_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5884682.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(2376..2756)
                     /gene="panD"
                     /locus_tag="OO330_004025"
     CDS             complement(2376..2756)
                     /gene="panD"
                     /locus_tag="OO330_004025"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5884683.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            3030..3932
                     /gene="rpnC"
                     /locus_tag="OO330_004026"
     CDS             3030..3932
                     /gene="rpnC"
                     /locus_tag="OO330_004026"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5884684.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(4006..4857)
                     /gene="panC"
                     /locus_tag="OO330_004027"
     CDS             complement(4006..4857)
                     /gene="panC"
                     /locus_tag="OO330_004027"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5884685.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(4869..5663)
                     /gene="panB"
                     /locus_tag="OO330_004028"
     CDS             complement(4869..5663)
                     /gene="panB"
                     /locus_tag="OO330_004028"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5884686.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(5776..>6091)
                     /locus_tag="OO330_004029"
     CDS             complement(5776..>6091)
                     /locus_tag="OO330_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5884687.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
BASE COUNT         1434 a         1460 c         1591 g         1606 t
ORIGIN      
        1 gcgtcatcat tttttggtgc caagttccag cgtaatattg aagagctgct ggtagcgccg
       61 gttccgactc acgtcattat tgccggatat gtcggcggtg gcgtggcgcg tggtctgttt
      121 gttggcattc tggtgacggc aatttcactg ttttttgtgc cgtttcaggt gcattcgtgg
      181 gtattcgttg ccttaacctt ggtgctcacg gcggtgctat tctcccttgc gggtttgctg
      241 aacggcgtgt ttgccaaaac gttcgatgac atcagcctgg tgccaacctt tgtgttaacg
      301 ccactcacgt atttgggcgg ggtcttttac tcactgactt tgttgccgcc gttctggcaa
      361 gggctgtcgc acctgaaccc aatcgtttat atgatcagtg gtttccgcta cggcttcctc
      421 ggtataaatg atgtgccgct ggtcactacc tttggcgtac tggtggtctt tattgtggcg
      481 ttttatttga tctgttggtc gctgatccaa cgtggacgtg gtttgcgtag ctaaggctat
      541 ttcctctcct ctggattcgg gggagaggag ttttgacggc tatcaccctt tatcaacaat
      601 ggtcagggta ggctgatttt cggctaagga ggaaggcgat gttaggttgg gtaattacct
      661 gtcacgatga ccgggcgcaa gagatactgg atgcgctgga gaaaaaacat ggggcacttc
      721 ttcagtgccg ggccgtgaat ttctggcgcg gattaagctc taatatgctc agccgcatga
      781 tgtgcgatgc tctgcatgaa gcggactctg gtgagggtgt catcttctta accgatatag
      841 ccggagcgcc accgtatcgc gtggcttcat tattaagcca caaacactcc cgttgcgaag
      901 tgatttctgg tgtcacgtta ccgttaattg aacagatgat ggcttgccgt gaaaccatga
      961 ccagttcaga gtttcgtgag tgtattgtcg aactgggtgg gccggaggtg agtagtcttt
     1021 ggcaccaaca acaaaaaaat ccgcctttcg tcctcaaaca taatttgtat gagtattaac
     1081 ccgcgattct gatggcgctt ttgctacaat aaaagcgttg tttcaccctc ggttattttt
     1141 tcatgtataa acaagctgtt attctcctgc tgatgctgtt taccgcaagt gtcagtgccg
     1201 cgttacctgc ccgttatatg caaaccatcg aaaatgctgc ggtctgggcg caaattagtg
     1261 acaagatggt gaccgtgggg aatattcggg ccggacaaat cattgccgtg gagcccactg
     1321 ccgcaagtta ttacgcattt aattttggct ttggtaaagg gtttatcgat aaagggcatc
     1381 tcgagccagt tcaggggcga caaaaagttg aagacggttt gggtgacctc aacaagccgc
     1441 tgagtaatca gaacttaatt acctggaaag atacgccggt ctacaacgcg ccgagtgtgg
     1501 gcagtgcgcc atttggggta ctggcggaca atttgcgcta cccgattttg cataaactga
     1561 aagacaggtt aaatcaaacc tggtatcaga tccgtattgg cgatcgactg gcctatatca
     1621 gcgcgctgga tgcccaaccc gataatggcc tgccggtgct aacttatcac catattctgc
     1681 gcgacgaaga aaacacccgt tttcgccata cttcgacgac cacctcggta cgcgctttca
     1741 ataaccaaat ggcctggctg cgcgacaggg gatacgcgac actgagcatg gctcagctgg
     1801 aaggttacgt gaagaatacg atcaatctcc ctgcgcgagc ggtggtgatt acctttgatg
     1861 atggcctcaa gtcggtgagc cgctatgcgt atcctgtgtt gaaacaatat ggcatgaagg
     1921 cgacggtgtt tattgttacc tcacgcatca aacgtcaccc gcagaagtgg aacccaaaat
     1981 cgctgcaatt tatgagcgtt tctgagctta acgaaattcg cgatgttttt gatttccagt
     2041 cacataccca ttttttgcat cgggtagatg gttatcgccg acccatatta ctgagccgta
     2101 gtgagcacaa tattctgttt gattttgcac gttcacgccg cgctctggcg caatttaatc
     2161 cacatgtctg gtatctttcg tatccgtttg gcggctttaa tgacaaagcc gtgaaggcag
     2221 caaacgatgc cggatttcac ctggcggtga caaccatgaa aggcaaagta aaaccggggg
     2281 ataatccgtt gttactaaaa cgactttata tcttaagaac ggattcgctg gagaagatgt
     2341 cgcggctggt gagtaaccag ccgcagggat aacaatcaag caacctgtac cggaatcgct
     2401 ttcgcggtac gtttcatttc attgtcgcct tcaaaatagg cgacattggg tcgccaggtg
     2461 cgagcttctt catctggcat ggtaacgaag ctggcgatga tgacaatatc gccgacgctg
     2521 gcgcagtggg ccgccgcacc gttaacagaa ataattctcg aaccgcgttc tgccgcgatg
     2581 gcataagtgg agaaacgctt gccgttggtg acattccaga tatcgatggc ttcgttttcg
     2641 agaataccgg ctgcgtcaag aaaatcctgg tcaatggcgc aagaaccttc atagtgcagg
     2701 tccgcatgag tcactttcac gcggtggagt ttgccctgca gcatcgtgcg aatcataact
     2761 tctacctttc taccctgtcg ttaacgaagc aggcggtgcc tgctttgagg aaattctcac
     2821 gcagtattgc ccgatttttg atgagtgtct actcatctga tggcatttgc gtcagcagtt
     2881 tgcgtaccgc gccgaagcgt gccatgtaac gcctgtaacg aattgatatt tttatgaacg
     2941 ctgctcggta aacaataaag aggtggctga cgatagcacg ccttgactgg atactgcctt
     3001 cacgcaaggc cacacggaag acagggaaga tggatgcacc gagtaccaca ccgcatgacg
     3061 cggtatttaa acaattttta atgcatgcgg agacggctcg cgactttctg gagatacatt
     3121 tgccagtgga attacgcgaa ctttgtgatc tcaacacgct tcatttagag tcggggagtt
     3181 tcattgaaga gtgcctgaaa ggtcacagca cggacgtgct ctattccatg caaatgcagg
     3241 gcaatcccgg ttatctgcat gttgtgattg aacaccaaag caagccggat aagaaaatgg
     3301 cctttcgcat gatgcgttat tctattgctg ccatgcaccg gcatctggag gctggccacg
     3361 ataagctgcc gctggtggtg ccgatactgt tttatcaggg cgaggccaca ccttatccgc
     3421 tctcaatgtg ctggtttgat atgttttact cgccggagct ggcgcgacgc gtctataaca
     3481 gtcctttccc gctggtggat atcaccatca caccggatga cgaaatcatg caacatcggc
     3541 ggattgcgat tctcgaacta ctgcaaaaac atattcgcca gcgcgactta atgttattgc
     3601 tggagcaact ggtcacgctg attgacgaag ggtacactag cggaagtcag ttagttgcca
     3661 tgcaaaacta tatgctgcaa cgcggtcata ctgaacaagc ggatttgttt tatggtgtgc
     3721 tgagagacag ggaaacggga gggaagtcta tgatgacgct ggcgcagtgg tttgaagaga
     3781 aagggattga gaaggggatt cagcagggaa gacaggagga aaggcaagaa ttcgccctgc
     3841 gtcttctaag taaaggaatg tctcgggaag acgttgcaga gatggcaaat ttacctcttg
     3901 ctgagattga taaggtaatt aaccttattt aagttacctg tgttatgaca gatgacgtgg
     3961 ggtaaattaa taactggcgc catcagccgt agcgccagtt aagtattacg ccagctcgac
     4021 cattttgttg tcgatcaggc gagcatcgcc aagccaggcg gctaccagaa ttactgcccg
     4081 tttgctggtt tcagaaactt ccagcaatgt gtcggcatcg cgaatctgaa tatcatcggc
     4141 gcggaagcct ttttcattca gttcttgccc cgcaatggta ataatttcat cgagatcccg
     4201 ttccccagcc tgcaatttgt cagcaatcga acttaaaact ttgtacagac caggcgcaat
     4261 tttgcgttgt tccgccgtca gataaccgtt acgggaactt agcgccagac cgtctttggc
     4321 gcgcataatt ggcacaccga caatctcaat atcgaagccc atatcggcaa ccattttgcg
     4381 gatcaacgcc agttgctgaa aatctttttc accgaagcag gcgatgtccg gctggaccag
     4441 gttgaacagc ttgctgacaa tagtcgaaac gccgcgaaaa tgtcccggac ggctggcacc
     4501 ttccagcatg gtcgaaaggc caggaacgtc aacgtaagtg tgggtttcag taccgttcgg
     4561 gtagatctct tttaccgaag gggcgaaaac taaatccact ttacgtttgt ttagcttctc
     4621 gcagtcctcc tgcaaggtcc gtggataacg tgccagatct tccgggcggt cgaactgcat
     4681 cgggttaacg aaaatactga cgacgaccac atcggcgcgg gctttggctt cgtcgaccag
     4741 cttcatatgg ccattgtgca gattacccat agtaggcacc agcgccacgc gcttgccttc
     4801 catacgcagg cggcgaattt gctgacgcag cagcggcagg gtttcgataa ttaacacaac
     4861 gtgactcctt aatggaaact gtgttcttcg cccggataaa cgccggactc cacttcagcc
     4921 atatactgcc gcacagccgc gcggatgtcg cccgtttcgg cgaggaaatt tttagcgaat
     4981 ttaggaatgt gaccgccggt aataccaaag gcgtcgtgca tcacgaggat ttgcccgtca
     5041 gtgacgttgc ctgcaccaat accaataacc gggatcgcca gtgcttcggt aatacgtttt
     5101 gccagctcaa ccggcacgca ttccagcacc agcagctgtg cccccgcggc ttctaaggct
     5161 aatgcatcgc tgagtagtcg atcgctcgct tcattgccgc gcccctgtac tttgtagcca
     5221 ccgaaaatat tcactgactg tggggttaaa cctaagtgac cacatacagg aacggcacgt
     5281 tcggtcagca tttttacggt ttcgaccagc cactcaccgc cctcaatttt gaccatattg
     5341 gcaccggcac gcataaccgt tgcggcgttt tcaaaggctt gttccggcgt ggcatacgcc
     5401 ataaacggca ggtcagccaa tagcaggcag tttggtgcgc cgcgacgtac ggcggcagtg
     5461 tggtaggcga tatcagcaac ggttactggc agagtggaat cgtggccctg aacggtcatg
     5521 cccagcgagt cgcctaccag catgacgtta agcccttcat cagcaaatag tttcgcgaag
     5581 ctgtagtcgt aagcggtgat ggtcgcgaaa cgctttttat cctgcttata tttctgaagt
     5641 gaagcaatgg tggtcggttt cataacgtat cctgataaat gatgtcgtgt tgcctggcat
     5701 tttatcagtc acattggtgg gggcaatgat ttatcaatta ccgcaccgcc agatgatggc
     5761 ggtgcggtgg acttattaag ggtaggttac ctggaaagta ctggtggctt taaactcacc
     5821 agcttctatt ggtatagttc catcctgttg tagcgtggcc tggaaatgga gagggtatgt
     5881 tatacctgta tcttgatccg ggtaaactcc tcctgttgta tcgtctttgc ctctcgggtc
     5941 gtaatcttta taaacagaat ttgaatcatt aggtttcaat gtcattagag gatttttact
     6001 agttgctaaa ccttctatga gtacaccgac tccttttgct gcatcattac ctgtgagagt
     6061 attaccaagg agttgcccgt tttcagtacc a
//
LOCUS       DAJTRB010000174         6065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTRB010000174 DAJTRB010000000
VERSION     DAJTRB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.174"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            381..1409
                     /gene="torT"
                     /locus_tag="OO330_004030"
     CDS             381..1409
                     /gene="torT"
                     /locus_tag="OO330_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5884688.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1382..2074)
                     /gene="torR"
                     /locus_tag="OO330_004031"
     CDS             complement(1382..2074)
                     /gene="torR"
                     /locus_tag="OO330_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5884689.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2171..3343
                     /gene="torC"
                     /locus_tag="OO330_004032"
     CDS             2171..3343
                     /gene="torC"
                     /locus_tag="OO330_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5884690.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3343..5883
                     /gene="torA"
                     /locus_tag="OO330_004033"
     CDS             3343..5883
                     /gene="torA"
                     /locus_tag="OO330_004033"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCR5884691.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5880..>6065
                     /gene="torD"
                     /locus_tag="OO330_004034"
     CDS             5880..>6065
                     /gene="torD"
                     /locus_tag="OO330_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5884692.1"
                     /translation="MTTLIAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIA"
BASE COUNT         1472 a         1517 c         1680 g         1396 t
ORIGIN      
        1 ccatttcggt cccagttagg acgctttctc atcaagccag tccgcccacc actgcatcat
       61 ctccctacgc ttatcgagat actgagcatg gttgtaaatt caattaaccc agtgcgttga
      121 caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt acaggtcact
      181 aataccatct aagtggttga ttcatagtga ctggatatgt tgtgttttgt cgcgtttttc
      241 agtctattat ttaatcgtta agtaatctaa tgctatgatt tttaaattat ttatgtgatt
      301 ttgtcatcct ttaggtgaaa aaggttgagt cgcaaagtgg aatgcatcta gcataaagcc
      361 ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc cttttcatgt
      421 tgtcggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat ttcacggtgc
      481 aagcctctat gccgcttaaa gccaaacgcg tatggaaact gtgcgcgctt tatcccagcc
      541 tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct cgccgctacg
      601 gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc cagcaagcac
      661 aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt agcacgacgt
      721 catttcccga cctgcaaaag caggtagcaa atctgccggt gatcgaactg gtaaatgctc
      781 ttgatgatcc ccaggtgaaa agccgcgttg gtgtaccctg gtttcagatg ggctatcaac
      841 ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact gaacgtgctg ctgatgcccg
      901 gacccgataa cgccgggggc agtaaggaga tggtcgaggg ttttcgcgca gccattgccg
      961 gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa atccagcgca
     1021 acctgttgca ggagatgctg gaacgccacc cagaaatcga cgtcgttgcc ggaacggcca
     1081 ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt accgtggtgt
     1141 cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg attatggctg
     1201 ccagcgatca aatggtctgg cagggggaac tggcagttga gcaggccatc aggcaattac
     1261 aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg ccgaaaaatg
     1321 ccgaccgaga acatatccgc cgctcgctgt caccgggggg atttcgtccg gtctattttt
     1381 atcagcacac atcagcggct aagaaataac cttcaccatg ttgcgtcacc agtaaatccg
     1441 cgctgagttt atgacgtaaa cgacgaatta acacatcgac ggtgcgcagg tcagggtttt
     1501 ccacccgacg cgcagaaagc atacgcagca gacgctcacg gctgagaatt tcgcccggat
     1561 tagtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc ggctcgccat
     1621 cccgctccag cgtatggcgc gacacattca ggcaataacc cgcaaagcga tagcagtagt
     1681 cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga tttttcaccc
     1741 gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg cccatttcca
     1801 gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc cccaccgttg
     1861 agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggtaag ttgatatcca
     1921 gcagaattaa atctaccggc tgattctgca taatttcccg tagcccggca ccgctcgccg
     1981 taacggaaac ggtatacccc tcctgagtga agtaggattg taatcgcgcc tgggtaaccg
     2041 gcttatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca ttctgttcat
     2101 atctgttcat atctgttcat atttgctcat taagatcgct tcactaaacc ataattctac
     2161 aggggttatt atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt
     2221 actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt
     2281 tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt
     2341 gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg
     2401 ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac tggaagcgag
     2461 caacgacatt taccagacct ttattgccca ctccattgat acacctgaaa aattcgaagc
     2521 caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca actcggcaac
     2581 ctgtcgctcc tgccataact acgatgcgat ggatcatgcc aggcagcatc ctgaagcggc
     2641 acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat
     2701 tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc
     2761 cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa tttatgcggc
     2821 gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga aagtccttaa
     2881 acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg ccggacgtca
     2941 gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt
     3001 gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc gacaccaata ccgagtggag
     3061 caagttgcag gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat
     3121 ctgggcctat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga
     3181 aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac
     3241 cagtctcgat aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga atgcgtctga
     3301 caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc
     3361 tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt cgccgggatg
     3421 ctggggccgt cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc gactgaggct
     3481 gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgatggtg
     3541 aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg
     3601 atcgccggat tgccggatca cgtacacaac gcggcacgta ttcgttatcc gatggtacgc
     3661 gtggactggc tgcgtaagcg ccatctgagc gacacctccc agcgcggtga taaccgtttt
     3721 gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga acgcgtacag
     3781 aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc
     3841 cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg
     3901 ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt cggttcaatg
     3961 gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa aaccattgtg
     4021 ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat
     4081 gtttatgaat attacgagca gctaaaagcg aaagtcgccg ccggtgaagt tgaggtcatc
     4141 agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca tgtgaagcac
     4201 attgcggtta acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca tacgctgtac
     4261 agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt tgagcagttc
     4321 ctgccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa
     4381 ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc ggcgaacaga
     4441 acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg
     4501 atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggttttggc
     4561 tggcactaca acggcgcagg cacgccgggg cgtaaaggcg ttattctgag tggtttctcc
     4621 ggctctacgt cgattccgcc tgttcacgac aacagtgact acaaaggcta cagcagcact
     4681 attccgattg cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac
     4741 ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt ttgccggaac taacccattc
     4801 catcgccatc agcagatcaa ccgcattatt gaaggctggc gcaagctgga aacggttatc
     4861 gccatagata accagtggac ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc
     4921 acgcagtttg agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc
     4981 gccatgaaac aggtggtgcc gccgcagttc gaggcgcgca acgactttga tattttccgc
     5041 gagctgtgcc gtcgctttaa tcgtgaagaa gcctttaccg aagggctgga cgaaatgggc
     5101 tggctgaaac gcatctggca ggaaggtgta caacaaggca aaggacgcgg cgttcatctg
     5161 ccagcgtttg atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg
     5221 tttgttcgcc accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt
     5281 ggcctgattg agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg
     5341 catccgatgt ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat
     5401 ccgttgcatc tgcaatctgt gcatccggat ttccgacttc actcgcagct atgtgagtcg
     5461 gaaacgctgc gtcagcaata tacggtagcg ggtaaagagc cagtgttcat taacccgcag
     5521 gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt
     5581 caggtgttgg caggagcagt agtttctgac cgctatgcac ccggcgtggc gcgaattcac
     5641 gaaggggcat ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg caaatacggt
     5701 aaccccaacg tgctgaccat cgacatcggg acttcgcagc tggcgcaggc gaccagtgcg
     5761 cacactacgc tggtggaaat tgagaagtac aatggaacag tggagcaggt gacggcgttt
     5821 aacggccccg tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca
     5881 tgaccacgct gatagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct
     5941 cccgtgaact ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gctgaatggt
     6001 tttcgttgct gaaaagcgaa ccgccgctca ctgcggcggt gaatgatctg gaaaacagta
     6061 ttgcc
//
LOCUS       DAJTRB010000175         6055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTRB010000175 DAJTRB010000000
VERSION     DAJTRB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.175"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            433..1341
                     /locus_tag="OO330_004035"
     CDS             433..1341
                     /locus_tag="OO330_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5884693.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OO330_004036"
     CDS             complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OO330_004036"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5884694.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKQAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3656..4414
                     /gene="loiP"
                     /locus_tag="OO330_004037"
                     /gene_synonym="yggG"
     CDS             3656..4414
                     /gene="loiP"
                     /locus_tag="OO330_004037"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5884695.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4620..5540)
                     /gene="speB"
                     /locus_tag="OO330_004038"
     CDS             complement(4620..5540)
                     /gene="speB"
                     /locus_tag="OO330_004038"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5884696.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWSFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5676..>6055)
                     /locus_tag="OO330_004039"
     CDS             complement(5676..>6055)
                     /locus_tag="OO330_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884697.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1577 c         1563 g         1424 t
ORIGIN      
        1 ttgctgaatg tataactgag attgatgatt ctggaaaata atcactaagc cgcataaatt
       61 atccttattt tatttttagg gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta
      121 atgaaagcaa ttgggtacgc gagaagaaaa accccacaaa ccaaaataat aaaaccttaa
      181 ataacaacaa tatgaaattg atatttttaa agtcaacttt gtatttgtta tattcatcac
      241 aaaatcaaca ttttgtttgt tattgtattt gtgatgcata tctctattta tttattgaat
      301 gttcgaaaac atacttttct aatgcaatgt tttttcgcta taccccttta tagattctga
      361 attctcacct gtcggctgtt ttctcctcat ctcatgtgac atattcccat actttactca
      421 aggaagattg tcatgaaagc acctgtttca ttaacctcat ttcgcccaca aaaatctctt
      481 gctatcgcca ttggcgtgct tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt
      541 aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa tcgtaaaagt tgccgaaccg
      601 gggctgggtt ttaagatccc ttttatggaa agcgtggaga aaatttccac gcgtaatcag
      661 gctgtggtct atcagggatt acaggcctac agccgcgacc agcaaccggc acaaatgaca
      721 gtatcagtca gcttccacat taaaccttca gaagcaggcg cggtttatac cacctacaac
      781 actattgaag cattaaaaga tcgtctaatt gtccgtcagt tgccaactca gctggagaac
      841 attttcggcc aatataccgc catcagcgcc gtacaggatc gtacaaaatt ggttcaggat
      901 ctgcaaaatg ccatgcgcaa agccgttgtt gggccagtgg taattgacgg ggtccagatt
      961 gaaaatattg atttttctga cgcttatgaa aagtcgattg aaaaccgaat gaaagcagaa
     1021 gtcgccattg ccacgcgtaa gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg
     1081 gtaactcagg cccaggcaga agcagacagt aaactggcgg cagccaaagc agaagcggaa
     1141 actattcgcg taagaggcgc ggcagaggct gaaactattc gtttaaaaag tgcggctgaa
     1201 gcagaagcaa tccgcctgcg cggtgaagct ctgcgggata atcccggact tgtagccctc
     1261 accactgccg aacgttggga cggtaaactg cccgatacca tgatccccgg cagcacagtt
     1321 ccgtttatct ctactaagta aaaaaagggc cgctttcgcg gtccttctca caaaacgtct
     1381 tactgattac agcagcgctt ttgctttcgc aacaacgtta tcaacagtga agccgaactc
     1441 ttcaaacagc agctctgccg gagcagattc accgaaggtg gtcatgccga cgatagcgcc
     1501 gttcaggcca acatacttgt accagtagtc agcaataccc gcttctaccg caacgcgtgc
     1561 agtaaccgct ttcggcagta cggattcacg gtaagccgca tcctgcttgt caaatgcgtc
     1621 ggtagacggc atggacacca cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc
     1681 agcaaccgcc agttcaactt cagaaccggt ggcgatgaag atcagttccg gctgaccagc
     1741 acagtctttc agcacataac caccgcgcgc gatgtttgcc agctgctctt cagtacgttc
     1801 ctgctgcgcc aggttctgac gggagaggat aagcgcagtc gggccgtcct gacgctcaac
     1861 gccgtatttc cacgcgaccg cggattcaac ctggtcacac ggacgccatg tagacatgtt
     1921 cggggtcacg cgcagagaag cgacctgctc taccggctgg tgagtcgggc catcttcgcc
     1981 cagaccgatg gagtcgtggg tgtaaaccat cacctggcgc tgtttcatca gcgcagccat
     2041 acgtacggcg ttacgtgcgt attccacgaa catcaggaag gtggaggtgt acggcaggaa
     2101 gccaccgtgc agggagatac cgttagcaat cgcggtcata ccgaactcgc gaacaccgta
     2161 gtggatgtag ttacctgcag catcttcgtt gattgcttta gaaccagacc acagggtcag
     2221 gttagacggt gccaggtcag cagagccgcc gaggaattca ggcaacagcg ggccgaacgc
     2281 ttcgatagca ttctgcgacg ctttacggct ggcgattttc gccggattag cctgcagttt
     2341 agcgataaac tctttcgctt tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg
     2401 ggtaaattca gccgcttcct gcggataagc tttcgcgtaa gccgcaaact tctcattcca
     2461 tgcagattct ttcgcctggc ctgcttgttt cgcatcccac tgagcataga tttcagacgg
     2521 gatttcgaac ggtgcatatt tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc
     2581 gcccagcggc gcaccgtggg agtcgtgagt accggctttg ttcggggaac cgaaaccgat
     2641 gatggttttg cacatcagca gggacggttt gtcagtcact gcgcgcgctt cttcaactgc
     2701 gcgtttgatg gatgccgcgt catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc
     2761 ttcgaaacgc attgctgtgt cgtcggtgaa ccagccttca acgtgaccat cgatagaaat
     2821 accgttgtca tcgtagaacg caatcagttt acccagcttc agcgtacccg ccagagagca
     2881 aacttcgtgg gagatgcctt ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg
     2941 gtcgacaatg tcgtggcccg gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc
     3001 cataccgact gcgttggcaa taccctgacc cagcggaccg gtggtggttt ccacaccagc
     3061 ggtataacct acttccgggt ggcccggagt tttagagtgc agctgacgga agtttttcag
     3121 ttcttccatc ggcagatcgt aaccggtgag gtgcagcagg ctgtagatca gcatggagcc
     3181 gtggccgttg gacagcacga agcggtcacg gtcagcccag gacggattct gcgggttgtg
     3241 tttcaggaaa tcacgccaca ggacttcggc aatgtcagcc atacccatag gggcacccgg
     3301 gtgaccggat ttggctttct gtactgcgtc catgctcagc gcacgaatag cattggcaag
     3361 ctctttacgt gaggacattt tgactccaga tcggatgatg aagggcacgc ccttaacgac
     3421 ttgacgacag cgcgttttgg gctacgccgg aaaatttgcc aacaatttac cgcaagccgc
     3481 gcgtcatgta catggaacat ccttttgccg cttcagaaat ctctggatca tgctcgcatg
     3541 ttgcgcaatc tgctcgcccg tccgctgcgc ttttccttat actgagactg agcgtcgatt
     3601 cacctgcaaa cggcgcattt ttagaataat cctgaccttg tgcggaagag aaaacatgaa
     3661 aattcgcgcc ttattggtag caatgagcgt ggcaacggta ctgaccggtt gccagaatat
     3721 ggactccaac ggactgctct catcaggagc ggaagctttt caggcttaca gtttgagtga
     3781 tgcgcaggtg aaaaccctga gcgatcaggc atgtcaggag atggacagca aggcgacgat
     3841 tgcgccagcc aatagcgaat acgctaaacg tctgacaact attgccaatg cgctaggcaa
     3901 caatatcaac ggtcagccgg tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt
     3961 tgcaatggct aacggctgta tccgcgtcta tagcgggctg atggatatga tgacagataa
     4021 cgaagtcgaa gcggtgatcg gtcacgagat ggggcacgtg gcgttaggcc atgtgaagaa
     4081 agggatgcag gtggcgcttg gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg
     4141 gattgtcggc agtttatctc aatcacaact tggtgatctg ggcgagaaat tagtcaattc
     4201 gcaattctcc cagcgccagg aagcagaagc cgatgattat tcttacgatc ttctgcacca
     4261 acgcggcatc agcccggcag gtcttgccac cagctttgaa aaactggcga agctggaaga
     4321 aggccgtcaa agctcaatgt ttgacgacca tcctgcatcc gccgaacgtg cccagcatat
     4381 tcgcgatcgc atcagcgcgg atgggattaa gtaaagccta gtggtgtcga acgtaggtca
     4441 gataaggcgt tcacgccgca tctgacatta atggcacatt tgttaccttg tgcgcattgc
     4501 cggatgcgat gctggcgcat cttatccggc ctacgggtgc cgaacgtagg tcggataagg
     4561 cgttcacgcc gcatccgaca ttaatggcac gttttacccg tgcgcatcgc atctggtgct
     4621 tactcgccct ttttcgccgc ctgaatatac agcatctcca gcgccagcgt cgccgcagcc
     4681 agagcggtga tttccgactg atcgtatgcc ggagccactt ccactacgtc catcccaacg
     4741 atgttgagat ctttcaggcc gcgtaccagt ttaatggcgc gatcggaggt caggccgcca
     4801 atcactggcg taccggttcc cggcgcgaaa gcaggatcca ggcagtcgat atcaaaagtc
     4861 aggtaaaccg gcatatcacc cacaatctgt ttcacctggg cgataacgtc atccacgctg
     4921 cgatcgttca cctggcaggc gtccagcacg gtaaagccgt tgtctttatc aaactcggta
     4981 cgaataccaa tctgcacgga atgattcggg tcgatcagac cttctttcgg cgcggtatag
     5041 aacatagtgc cgtggtcaaa ttcacaaccg ttcgcatagg tatcggtgtg ggcgtcaaag
     5101 tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca
     5161 aagtggtcac caccgaagga gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc
     5221 gcctgcagct tttcgctcat ctcacgggca tcgccaaagg catataccag atcgccgcag
     5281 tccacgacgt tcagacgctc acgcatgtcg aaactccacg ggaagcggtt gtgttcccag
     5341 gccagattcg tcgaaacctg acggatcgct gccggaccgt ggcgaccacc cgcacgacca
     5401 gaagtggcca tatcgaacgg cacgccagta atcacccagt ctgcatcgct gtcatacggc
     5461 tggaagttca tcggcaggcg taaaaaacca aaggcattgg aaaccagtga gttatcgtat
     5521 tgatgaccta aggtgctcat ggatggactc ctattacaaa gtcgatataa aaaaaccctt
     5581 ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga aacacaagtt tgtgctaatt
     5641 atcgccttaa atgggcgagg catcaagcga ggttgctatt tccgacacgg tggcggcgca
     5701 tagccattgt tatctgccgg aaaggcttgt ccagagcacg gcggcagcgc gttttgcgcc
     5761 gggccttgta attcaaaggc ggcaaagccg attaccccgg tattacgcac atcgccttgc
     5821 gccgaattgg ctgcgtagga atcagcaacg ttggcgaact gaaaagccgc ttcagtatgt
     5881 ttatctttgc ggaatccttt aatcactaac gaatctccgg cattcacaat atagccgttg
     5941 ttattcagcg aaccttgctt gccgttcagc acatccaggc catcagccgt agcaacgatc
     6001 tcgtagttgg tgttacgact gtaattacgg acatataatt gataattcat tccga
//
LOCUS       DAJTRB010000176         6012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTRB010000176 DAJTRB010000000
VERSION     DAJTRB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.176"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            526..1704
                     /gene="ltrA"
                     /locus_tag="OO330_004040"
     CDS             526..1704
                     /gene="ltrA"
                     /locus_tag="OO330_004040"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5884698.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1787..1966
                     /locus_tag="OO330_004041"
                     /pseudo
     CDS             1787..1966
                     /locus_tag="OO330_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1996..2478)
                     /locus_tag="OO330_004042"
                     /pseudo
     CDS             complement(1996..2478)
                     /locus_tag="OO330_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2734..3714)
                     /locus_tag="OO330_004043"
     CDS             complement(2734..3714)
                     /locus_tag="OO330_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5884699.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3714..4913)
                     /locus_tag="OO330_004044"
     CDS             complement(3714..4913)
                     /locus_tag="OO330_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5884700.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5235..5573)
                     /locus_tag="OO330_004045"
                     /pseudo
     CDS             complement(5235..5573)
                     /locus_tag="OO330_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5640..>6012)
                     /gene="istB"
                     /locus_tag="OO330_004046"
     CDS             complement(5640..>6012)
                     /gene="istB"
                     /locus_tag="OO330_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5884701.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT         1638 a         1376 c         1426 g         1572 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtggaggtgg gccctccggt cagggtttac
       61 aggagcacag accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt ggggttcggg aagctgaacg aaagtaaact
      181 gtccgatgaa gcatcgaaat cttagatgat gccaaaatgg ggccgtacag ccgtcccctg
      241 ataagcgcag aagacacctg cttactgact gcgcggcatc cggtgtgaag gcagcaggat
      301 cccgggccag accatctcag ggaacgtgag aacctgtttc cggcacgtat cgggaaacag
      361 gggcggaccc attcgtagta gtgatgaaag atctgtaatg ggtctggagc gaagggatgg
      421 ggatcacatt cattgcagaa gacacaaccc aaaaggggat gacgtaactg atgcaacgaa
      481 aatcatttga aataccaaaa gcactggtct gggcatcata tctggatgtg cgtcgcaaca
      541 agggagcccc ccggatgcga cgggcagact ttgaaaatgt tcgaccagca acgtgatggc
      601 aatctataca agatctggaa ccggctatgt tcgggaacat ggtttccgcc accagtgctg
      661 gaaaaacgga tcccgaagtc gaatggcaag gaacgcattc tggggatccc aacagtatcc
      721 gatcgaattg ctcagggagc gataaaactt ttcatggaag aaaagcttga cccgattttc
      781 catgctgatt catatggtta ccgaccaggc aaatcagctc atgacgctct gaaacaatgt
      841 gccatccggt gctggcgtta tagctggata ctggaagtgg acatcagtgc gtttttcgac
      901 catgtgagac atgatctggt gctcaaagct ctggaacacc acgggatgcc taaatgggtc
      961 atcctgtact gtcgacgctg gatggaagcg ccaatgcaga gttgtgaaaa tggagaatta
     1021 ataacccgaa cacgaggcac tccgcagggc ggggtcatta gtccgctact tgctaacttg
     1081 ttccttcact atgcatttga tttgtggatg gaaagagaat atcggggggt accgtttgag
     1141 aggtacgctg acgatattgt agtacattgt tcacgaatga gtgatgcaac aaggctaaag
     1201 aacaggttat cggagcgctt ctcggaagtc gggctggtcc tgaatgcagg gaaaacgaat
     1261 atcgcctaca ttgacacgtt taaaaggaga aacgtcgcaa cgagtttcac ctttctcgga
     1321 tatgacttca aagtgcgtac gctgaagaat ttcaaaggcg aacggtaccg aaaatgcatg
     1381 ccgggtgcgt caaatgcagc aatgcgcaaa ataacagaaa caatcaagaa gtggcgtata
     1441 catcgctcaa cagctgagag tttgctggat tttgcgaggc gctacaatgc gatagtgaga
     1501 ggctggatcg agtactacgg aaagttctgg tccagaaatt tcaactatcg actgtggagt
     1561 gcaatgcagt cacgtctgct caagtggatg cagtctaaat acagactttc gaaccggaag
     1621 gctcagcgga agctgacgct ggtaaggaag gagtatccga agctatttgt tcactggtat
     1681 ttactacgtg catcaaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1741 tccgtgagca gccggggagt gaaattctct ccggctgact cgactcgcgg gttccgacag
     1801 cgatgacgaa catgcggcgg tgttgtactc gctgatcggc acatgccgtc tgaacaatgt
     1861 ggaaccagaa aaatggctgc gttacgtcat tgagcatatc caggactggc cggcaaaccg
     1921 ggtacgtgat ctgttgccct ggaaagttga tctgagctct cagtaaatat caatacggtt
     1981 ctgacgagcc gcttactttt ggcgtaattc tgctgcgcct ttactgaaca caatgccgta
     2041 ttcagcaaga agtccacgaa tgtgatttat cagttgtgtc ctttccattg aaaccaaccg
     2101 acgcatattc aaaaaagtct ggacgtcctg ttgctccaga gtttttattg gtacgggtcg
     2161 tatagttcca tgttggcagg cctcagctat tgcctgtgcg tcgttataat catttttctg
     2221 gcaccgctga taagctttaa catgttgtgc cggaagcaat attacatcat ggtcgagttt
     2281 cctgatttcc cgggcccagt aatgagatgc cccgccagcc tccatcgcaa cagtcatttt
     2341 aggctgttgc accagccagt taagcagttg attgcgagaa agtttggtct ttcctgcggg
     2401 tttgccatga gcattgatac tgaaaagata aaagttcgtt ttagccagat caatgccggt
     2461 gattttctgt tcagacatgg tatcccgctt acttttgttg ttaaagttta tccatcatgg
     2521 cgcaattgaa ggctgccgtg aaagaggggg aagactatct catcggttac tgttaaacgg
     2581 ctaagagaaa ctatcttttt caagcaaaga agatacggtg aaacaggcga tttcaccgtg
     2641 aaatactgtt caaaaaaaac gctaataaaa gcaaaatttc ttttaaaaca gccaattaaa
     2701 aactggtgag ttgctggcga aaaacttgaa ttttcacagc gaattcagat tatcttttag
     2761 tacaagagca atcatacgat ctaacttatc ctgaacttcc aacggtaatc tgccgaattc
     2821 atatgagaac gtacgtccct ttactcgttt tcgagcaaat ttatctttac tatcaaattt
     2881 ccataacaaa gttacaaccg ctttatcctt tactcctgaa tcaagcaatg aagacatttc
     2941 cttggttata agccgcataa tactgttttt aacctcgtct tcagaaagat tgtcatcagc
     3001 agtaataagc tcaatactcg gcgagatatt cttcacaact tcatcaatag atatatcacc
     3061 cagcctcagt cgttcactta attcagccag ttgcctgtaa tcggcgtatg tcagctcaga
     3121 ctgaacagga aaaagagaca caaaatcttt aggtacggac gctgcttgga gggcacgagt
     3181 caccttagct gccgacaatc cctctctttc cgcaatttgc ttctggttca taccggcctc
     3241 tttgagacgt actaaacgca acccaatttc acgaatatta tgttcaagtg acgtctgcaa
     3301 atctttcgcc agatgctgag cttcagaaac agtaagctca tcatccgtta caagtacacg
     3361 taatccaacc ttgcatagca aagctgctgc ccgacgacgg gaaccatcca aaatctcaat
     3421 aagatcgcca gtccgaatcc caatacatgg atagaattgt tgtaaacgta tagttctggt
     3481 gatgtctttt agtgagtccg gcgtcagggc tgtctgttcg cggccattca cctcttcaac
     3541 cacaaaagtt tgagtatcca ctttgtcatg atcaaccttg atctcagtaa attttgcctt
     3601 tcggccagta ttgagggtaa atacatgaac tggagccgat atttcttcag tattattcag
     3661 gatatttgta ttcagcgtac ggccaatagt cggtcggtgc tttctgtttt ccatcagtag
     3721 ttggccctta taaattcaat acgatcaaaa actgctttag cgaaatcttc tgctgccata
     3781 cgtgcatttt tcaaagcttc accactgcca acataagtaa caggattcgc agatatcacg
     3841 gtatcaaacg actcgccgct tctctcaaaa ccatcaagac gtggcattga aacatcaagc
     3901 atgtcaccac cgaaaatctc ttttgtcagg ctatggcagt atttatgatc tgacttattc
     3961 gccagtttcg acatgaagcc aatatttgcc tgcaaacggc atgaacatcc atcctgttca
     4021 ataatctgta caagttcagg taaacgagcc aaatatttaa gcgtggaatg gaaatcaact
     4081 tgtgctggcg gcaccggtgt aaacatgata tctgcggcag caatggcatt tttcaaaaat
     4141 gcgtccagat gcgggcctgt atcaataagg atgaagtcga aatcatgctt cagcttatca
     4201 ataatattct cacgcaatat cgcgtgtttg ttttgtccta atagatgctc ctcgcacaga
     4261 gtatcccagt ttgatgcaat aaaggcgtcg tcaatagaag ccggcataac ataaacgcca
     4321 gggataactg acggaacgat aaaatcttcc agtaattcct cacgactcac gttctgcaac
     4381 atagcctgcg gtgcggttgt atcaaccagc cccaccgcat gcaagtagtt cagaaacata
     4441 gtggctgatg actggggatc taaatcaagg agcagaatac gtaaatcttc tgacagcagg
     4501 tgaggatgag cacgcagggc gtgagctacg gaaacactac tcacggtttt agacacaccg
     4561 ccttttagac taccgatgaa tatggaatat gcctcactat aacgatcgcg atatttagga
     4621 atgccacgat gtgcatagat atcgataatg ttctggatgg ttaatgcata ttttgtcgcg
     4681 gttcctgctg ggcgtttttc aaactgatag ccctgagcct ccatctcacc gactgcacca
     4741 tccacgcttg cgcgcgttaa ttttggaagc ttagctacag cagactttga atagacttga
     4801 taaaaaacat cagcctgaaa ctcctgcttt tgctcctgaa tctgttttgt tagggcgagc
     4861 aacattttat ctgcacgctg cgcaacctta cttaactgct caaaactagt cattccccct
     4921 ccgtgctatg taggatttta acttattaca aaaaaaactg cattagcagg gtaatgcatg
     4981 caagtacata ttgtacatgc ttatattaaa aatgcgcgca ttgctgattt attaatcatc
     5041 ggaatgaata ttactgtaag atagtatgcc tttccgggag aaagacgctg atttcccccg
     5101 aataatccag ttcaccacca cactccggac aacagctttc ttcaggctcc agatggtgta
     5161 tttcgcggga aacatgttcc ggtaacggac ggcgatgacg tgactgtcgc aacaggcggg
     5221 ggtagtgctt ccaatcagta gaacatgtgc ttttcaatga aggagccgat gactttttcg
     5281 tggatttcca cggaacgcga gaagcagatt gttttacgtg ccagccgctt gatgcgggtg
     5341 cggagggtcc ggttgttacg tgcaatacgt tgcgtgaaga ttttgccggt caggtgtttc
     5401 tccttcggca cttctctggc gtagctgccc cagtcatcgc tggtcaccat gccgatacag
     5461 aacggagtca acagggctaa cagttcccga caggtctcat cattccgtgg cccgaaagtg
     5521 taagccaaca caccgccagt tttagtgttg tacgcatacc agagccagtg ttgtgtcaac
     5581 gacggatgaa aagtgatcca cttatatctc caccaacggc ccaatattga tccaccgttt
     5641 tactcaggat tagcttctgc tataaccccg gcctttcgtt tctgtctgag tcgatagctt
     5701 tctcctttga tttgaacgac atgtgagtgg tgtaagatac ggcccagcat cgctgaggtc
     5761 agtgctgcat caccggcgaa cgtttgatcc cactgcccga acggtagatt ggatgtcagg
     5821 atcattgcgc tcttttcgta acgtttagcg atgacctgga agaacagttt tgcttcttcc
     5881 tgactgaacg gcagatagcc tatttcatca atgatgagca ggcggggggc cattactcca
     5941 cgctgaagcg tcgttttata acggccctga cgttgtgccg tagataactg aagtaacaga
     6001 tctgctgctg tt
//
LOCUS       DAJTRB010000177         5983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTRB010000177 DAJTRB010000000
VERSION     DAJTRB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.177"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..393)
                     /locus_tag="OO330_004047"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO330_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO330_004048"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO330_004048"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5884702.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO330_004049"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO330_004049"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5884703.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="OO330_004050"
     CDS             5797..>5983
                     /locus_tag="OO330_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5884704.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1839 a         1354 c         1019 g         1771 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgtcccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg tagattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAJTRB010000178         5882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTRB010000178 DAJTRB010000000
VERSION     DAJTRB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.178"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            65..583
                     /gene="mddA"
                     /locus_tag="OO330_004051"
     CDS             65..583
                     /gene="mddA"
                     /locus_tag="OO330_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5884705.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            580..1029
                     /locus_tag="OO330_004052"
     CDS             580..1029
                     /locus_tag="OO330_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5884706.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1030..1263)
                     /locus_tag="OO330_004053"
     CDS             complement(1030..1263)
                     /locus_tag="OO330_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5884707.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO330_004054"
     CDS             complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO330_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5884708.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1717..1812
                     /gene="yncL"
                     /locus_tag="OO330_004055"
     CDS             1717..1812
                     /gene="yncL"
                     /locus_tag="OO330_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5884709.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2214..3023)
                     /locus_tag="OO330_004056"
     CDS             complement(2214..3023)
                     /locus_tag="OO330_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5884710.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSELPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO330_004057"
     CDS             complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO330_004057"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5884711.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4679..5473)
                     /locus_tag="OO330_004058"
     CDS             complement(4679..5473)
                     /locus_tag="OO330_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5884712.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5463..5810)
                     /locus_tag="OO330_004059"
                     /pseudo
     CDS             complement(5463..5810)
                     /locus_tag="OO330_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1539 a         1491 c         1309 g         1543 t
ORIGIN      
        1 gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt tattgaggcc
       61 agacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
      121 ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
      181 cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga
      241 tggcgtagtt acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
      301 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
      361 attgttaagt cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
      421 gatcgaatcg caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac
      481 cgcacaaatg ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca
      541 gttgcaactc gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt
      601 gcttttttga ttgccgccgg aattggtctg gtagtccaga acacgctgat ggcgcgcatc
      661 acccagacct cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg
      721 ctctttgttt ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc
      781 agcgtgcgct ggtggacact tatacctggc ttgctgggtt cgttttttgt cttcgccagt
      841 atcagcggat atcagaatgt gggagcggcg acaactattg cggtactggt cgccagccag
      901 ctgattggcg gtctggtgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg
      961 ttcggcccaa tatgtggcgc aattttactt gtggttggcg cctggttggt ggccagacgc
     1021 tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc
     1081 ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta
     1141 gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg
     1201 ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga
     1261 catactttct ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc
     1321 gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga
     1381 aactcatcgg ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat
     1441 cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa
     1501 gcatatgttg atagagagac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa
     1561 tatgcgccat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa
     1621 cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta
     1681 ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta
     1741 gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc
     1801 tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcaactctct
     1861 gacagtacgc aagatcttct catactctcc aagtttgcaa cattatcctt aatatttact
     1921 tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg tcgtctatac
     1981 aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatcac gaatacttta
     2041 tacgcgcggg agtctataaa tgctttatac tttcaaggaa taacgtaaat ataaaaagat
     2101 ggcgtaaagt atatgcatgg caggatgttt gcaacctcct tgatgacgct gccccattga
     2161 aattgcaaac cccgccctgg ttatcttgcc catttatcat gcaaaacaga taattatagg
     2221 cctctgaata cctcagaaag attgaatttt ttatagtccc tctcttgaat atttccattt
     2281 gatatcaatc gattcatctc ttcaacaaaa taatttatat catcacactt attattatcc
     2341 ggaagttctg agtagttata attccaccat tttattctca acagttcatc aataacattt
     2401 gaatcaaacc taaatctgat tatttttgca ggaactcctg caactatggc atatgggggc
     2461 acatctttgg taacaacgga attggcagca ataacagcac catcacctat tgcgatgcca
     2521 cctttcaata caacatcatt tcctatccag acatcatttc ctataatcgg attttcaggt
     2581 ttggtaatga atggtttcaa tgtccagcta cgttcaaatt cactcatagc aaacttatca
     2641 aattcaccgt tataagtcat catgtgagta gtaaaacgat tcaaaggatg atttccaccc
     2701 attattttta catttgatgc aatactacaa aatcgaccta tacgtatatt ccctgaaaaa
     2761 tggcaggtcg gaaatgaaaa agcgccgaca ctggagaaat ttttcacagg cattgttgaa
     2821 tattcttcag caataacatt atcttcaatt gttatcttgt caccttttga gaaaagaaat
     2881 aaattccttt ctttatacga cttcttcccc ttatgattta aaaagatttt attatccata
     2941 caaaactcaa agtgtttttt cgtccatctg aaggtaaggg ttttattttt atcttccgtg
     3001 aatttgtaca attgaacttt cattgttaat acttctactt ataaagtatt agtttaataa
     3061 cgatactcta aagccttatg ctaatcaact gtgacaggat gaaaaatcgc catcaaacat
     3121 ttagcctaat ttttacgcca gttattaatg taatcattct aatacttaac cgccattaaa
     3181 ctcagaacct aaaacgatag tggccgtcct ataacagcca ctaacatcta acttaatgtt
     3241 taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac atatccttgc
     3301 cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg aaatgggtat
     3361 tgacccaggt acaaccatat tgcagccgtg cgctgacgcg atgcgccctg cccacatctt
     3421 tcgtccatac cgaagatgca agtccgtact ggctgtcatt cgcccagttc accacctgtt
     3481 cttcgttgtc gaagggcgta acactcacta ctggaccaaa tacctctttt tgcacgatgg
     3541 catcgtcctg taatgcgcca gccagcagcg tcggcgcata gtaatagcca ttacccttgc
     3601 gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct tctactgcct
     3661 tgctgacgcg ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta gactcgtcat
     3721 ccggcgcacc agattttaac gttgccaccg cagcacccag tttttccacc agcgtatcgt
     3781 aaatgccttt ttgcgcgtag atccgacaag ccgcagtaca atcctgtcca gcattgtaat
     3841 agccaaatgt acgtacacct tcgaccactg cttcaatatc cgcatcatca aaaacaatca
     3901 ctggcgcttt gccaccaagt tccatatgag tacgcttaat ggacgacgcg gtatggctga
     3961 tgatgtgctc gccggtggcg atagagcccg tcagcgacac catccgcact ttgggatgac
     4021 cggtcagcgg atcacccacc gttttgcctc tgccaaacag tatgttaatc acgcctgccg
     4081 ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta atttctgatg
     4141 gtttaagcac tacgcagttc cctgccgcca gcgccggagc aagtttccac gcggccatca
     4201 tcagcggata attccacggt gcgatagaag ccacgacccc caacggatcg cgacggatca
     4261 tcgaagtatg accttcaaga tattcacctg ccgccagacc attcagacag cgcgccgcac
     4321 ccgcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc atcattgaac gcactatgca
     4381 gcggtttgcc acaattacgg gattccagtt cggcaaaaac ctgaccattt tcttcgataa
     4441 catccgccag tttcagcagg cattccgcac gcgctttcgg tgtggtttgc ccccattcag
     4501 caaatgctgc atctgccgca cgcacagcag catcgacctg ctctgcggat acctcggcaa
     4561 tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc ccttcgccgc
     4621 taacccgttc tccgttaatc agtaacttat gttgcatagc ttattcctgt atcagtggtt
     4681 attgaccatt gtcgccttcg cgggttagcc accaggcccc caggatcggc aaggttgtta
     4741 ccaacataac cagcagtgcc accacgttgg ttaccggtac atcacgcggt cgcccaagct
     4801 gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaggtc gtaacgatga
     4861 tttcatcgaa cgacaaggca aacgccagca ttcctcctgc cagtaacgcc gaactgagat
     4921 tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc attgacgcct
     4981 caaccagact ccacgaggtg cggcgaaaac gggcgatgac attgttaaac accaccacta
     5041 cgcaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga ttgatggttt
     5101 taaaggcggt taatagcgcc agaccagtga caatgcccgg cagcgcaatg ggcagcagca
     5161 ataacagcga aatggcgttt ttgccgaaaa agtctcgtct ccacagcgcg gcagctgcca
     5221 gcgtccctaa caccagcgca attaatgtcg ccagcgccgc cactttaagt gacagcgtca
     5281 cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc gtcaggccct
     5341 gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcggcg attatcagga
     5401 tgggaaaatg taggaaaaca acgccgcccc aggccgccag tttgaggaaa aacggtgcgc
     5461 gttcagagtg catcgaacgc tcccagacgt ttcacgaacg ccaggtacag tgcgatgaga
     5521 ataatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc cccctgctgg
     5581 gaataaacca tattgccgat aaaatatcct ggaggaccaa ccagctgcgg gacgataaaa
     5641 tcgcccagtg tgagtgagaa ggtaaagata gagccagcgg caatacccgg gattgccagc
     5701 ggcagcacca catagcgaaa ggtttgtcgt ggacgtgcgc cgagatcagc cgacgcctgc
     5761 aacaatgacg gcggcaaacg ctcaagcgcc gcctgaacgg gcaggatcat cgagtaggca
     5821 gcctgacggc tgcggcttgt catggtctgc aattaccgtt ataaaaacag gcaatgtcat
     5881 tg
//
LOCUS       DAJTRB010000179         5802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTRB010000179 DAJTRB010000000
VERSION     DAJTRB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.179"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO330_004060"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO330_004060"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5884713.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO330_004061"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO330_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5884714.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO330_004062"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO330_004062"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5884715.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /locus_tag="OO330_004063"
     CDS             4129..4884
                     /locus_tag="OO330_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5884716.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="OO330_004064"
     tRNA            4963..5036
                     /locus_tag="OO330_004064"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5258..>5802
                     /locus_tag="OO330_004065"
     CDS             5258..>5802
                     /locus_tag="OO330_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884717.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
BASE COUNT         1460 a         1420 c         1474 g         1448 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaatttaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccaattt
     5041 atcttcacct gatcaatctg ccatttttgc tgtatctgat tttctatatt atcttaattc
     5101 aatcacttca ggcttttcgc aatcgctgta acacttcccc atttttttgt aaataaaaag
     5161 cgtttacgca atgcaatcct tttgtaacaa cccaggtaaa ataccttatt ttcaagttgt
     5221 tgaataagtg gttatctacg cttttaacaa acgattgatg gattatttta tgaaagggaa
     5281 atctgcatta actcttctgc tcgctggtat attttcgtgc ggcacatgcc aggcgacaga
     5341 ggcagaagtg acaagcgaat ctgtatttaa cattcttaat tccagggggg cagcaacaga
     5401 taaaagttat ttatcgttaa atccagataa gtatcctaat tatcgtcttc tgattcactc
     5461 agccaaatta aaaaatgaaa taaagagtca ctataccaaa gacgaaactc aaggattgct
     5521 aacattaaca gaaaacacac gaaagttaac gttgacggag aaaccctggg gaacgtttat
     5581 tcttgcttct actttcgaag atgacaaaac agcggcagaa acccactatg atgctgtctg
     5641 gctgagggat agtttatggg gctatatggc attagtatca gaccaaggaa atagtgtagc
     5701 cgcaaaaaag gttctgctca ccctgtgggg ttatatgtct acgcccgacc agattaagcg
     5761 gatgcaggac atcattagca acccaaaacg actggatgga at
//
LOCUS       DAJTRB010000180         5722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTRB010000180 DAJTRB010000000
VERSION     DAJTRB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.180"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            86..496
                     /locus_tag="OO330_004066"
                     /pseudo
     CDS             86..496
                     /locus_tag="OO330_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            528..1142
                     /gene="istB"
                     /locus_tag="OO330_004067"
                     /pseudo
     CDS             528..1142
                     /gene="istB"
                     /locus_tag="OO330_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1311..5405)
                     /gene="sepA"
                     /locus_tag="OO330_004068"
     CDS             complement(1311..5405)
                     /gene="sepA"
                     /locus_tag="OO330_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5884718.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1414 a         1434 c         1362 g         1512 t
ORIGIN      
        1 gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact
       61 ctccaggaaa cccggggcgg ttcatgccgt caggcagcag cgaagcaaag cgttactgac
      121 gtcgctgaat gaatggatgg tggagaagaa tggtacgctg tcgaaaaaat ccagactggg
      181 cgaagcgttc agctatgtac tgaatcagtg ggatgccctc tgttattaca gtgatgacgg
      241 tctggcggag gcggacaata acacagcaga aagagcgctt cgtaccgtct gtctcgggaa
      301 aaagaattac atgttcttcg gcagcgatca tggaggcgat cgcggagctc tgctgtacgg
      361 actaatcggg agctgtcggt tgaacggtat cgatccagaa gcgtatctgc gccatatcct
      421 gagcgtactg ccggaatggc cttccaaccg agttgacgaa ctgctgccat ggaacgtagt
      481 tctcaccgat aaataagcgt caatacggcc ttccgttgac gcttacagaa ctcctgtggc
      541 gcgagagtga aaaactgcgt cgttataaaa aggaggcccg gttgccagtt gccaaaacgc
      601 taagcgaata cgacttcatt cagttgccgg aactgaatgg agctcagttc cagcaactct
      661 gtgaaacgac agactgggtt gatgctggag aaaacgttct gctgttcggg gccagcgggt
      721 tggggaaaag ccatctggcg gcagcgatcg tggatggcgt agtaggccag ggctaccggg
      781 cccggttcta cagcgcagga gagctgttgc aggaactacg taaagccaga gctcagttga
      841 aactgaatga gctgctactg aaactggatc gctaccgggt gatagtggtg gatgatcttg
      901 gctatgtcaa acgcgacagc gccgaaacgg gtgtgctgtt cgagttaata gcgcatcgct
      961 atgaacgtgg gagcctggtg ataaccagta accatccgtt cagcatgtgg ggcagcatct
     1021 tcgtggatga gactatggcg gtggcggcag cagaccgact gatccatcac ggatatatgt
     1081 tcgaactgaa aggggaaagc tacaggaaaa agacagcgaa ggcagtaaca agcgtgactt
     1141 gatgtcgcac tgaaggggcg gccagtatag ttggcgccag tcggcaaaac tagttgacgt
     1201 ctaatacctg gacggtcact tctgagaaaa ggcatttaca cagaatctta aacaggctca
     1261 cggagttctt tcagaaaaag gcctgccgaa tggcagacct atcccattcg tcagaaaaca
     1321 taacggaagt tggcgtttat cgcattatcc acattatatt tcccgaacgc tgatttctcc
     1381 agctccagtc ccaaacgcat attatcctta atttccgcat tcatccctac cgtcatcagc
     1441 atcctgctat ctttttcacc ttcgaaacgt ttctctccgg aagcatcctg cagaaccgtt
     1501 tctccgttcg ccagcaggtc gaactgataa cccagcccgg ctcgagctgt gattttccag
     1561 tcgtctccgg agaaggctct tcccacgtca acaccagtac ggccaatcag tgggttataa
     1621 tccttgtctt tcatgctcag agccattccc cggtcttccc agctaaaagc ttttcctgat
     1681 acagaaccgt aaaccagctc tatctgtggc tccacccagg actctttcgt caggtggtaa
     1741 cgataaccaa cttcagcacc ggcataccag gaatgagagc tgtagtcttt tgttcccagt
     1801 gaggcaaaat tcgccgtgtg ctgattatca tgatggagat atttaccaat caggtcaaaa
     1861 taagctccgg aattaaagag tgcagaggca tacagaccgc cacccacgga tttgtttttt
     1921 ccactgaatg cgtggctgct tgcattgctg tccgtatagg tcagcaatgc ccccgtaaat
     1981 aaatccacac cgtccagctc atgctttctg tcgacaccaa tctgaacgtg agtgtagtta
     2041 tcgctgtagt caccatctgc cgaaccggta ccattcatta tgcgtgccca gacaccggca
     2101 tccccctgag tatcccgcag gtcacccata cgtttgttca ggttattgac ctctgtaagg
     2161 aaggacttat aacctgatgc cataaagtct tttgcggctt tcgaggctcc ggcatcagcg
     2221 gtagtctgat atcctgtcag cacccatttt gtggcatcat ccgttttttc cgtgctgatt
     2281 actggcgtaa cgttactgaa accaatctgc tgcgtaccgg cagtgaacgt tttctcatca
     2341 ctccctttcg gtgcggtaat taacgtcacg ctcatttcac tggctggttt gtttaaaaag
     2401 tcaaccagga cgctattatt actgcctgac agtttgtctg tgacattcaa ctggtcagcc
     2461 cggctgttgt tggtatgcat caggaacgtg gtattatctg cagaaagctc ttttacttgt
     2521 aaagtggcaa agtgtttccc gtcccccagt tcaattcgcg agcccttagc cttcagtgca
     2581 ccggcctctg attttccgtc agagtaccac agagcattga tcatatttac cgtactctgt
     2641 gcccccgtaa tactgcctct cagaacagcg ttatatccct tcattaagga gctgaataac
     2701 ggagagtccg tcttccctgc atcagcatca ctgtctccca gattaatggt tgccgcagtg
     2761 gttcctgcat gaatatccga actcagatag cccatatttt taacagaaaa gctggcgtta
     2821 tcttcgaggt taatcccttc tgttgtagaa attacgggtg agtaataccc ctgtttctgc
     2881 gcagaaggca tccccgtcag ggtcagagaa gcattttctt ttacatccac agccccggtg
     2941 ctgaaaatac cgctctgagc cgtaagtttt gctccttttc cgagggtcag gctgctgttg
     3001 acaaagctgc caacacggtc aaaaacagca ttctgagagg tcacactcac ggtgctatca
     3061 taagcctcaa taccaccggt gaatctctcc atgatttgca gggatgattt attttccagc
     3121 gtaacatttc cgctgtaatc actctggtca ccaactgtgg ttgcagtggc actcccttca
     3181 acagaggaga ccgcattacc ggttccgtca ctcctgtcag taaagacacg gctacttccc
     3241 agaatgactg tacttccatt ggtggcgtta atatctccct gcatcttgcc gtttctggac
     3301 agatggaatg ttgccgcatc gagtttcagt ttttccagcg taaactgacg ggtttcccag
     3361 tcgctctggc tggaagaagt cgtcgttcca gcatgaataa ctggatgacc ctggaatatc
     3421 agcgtgccgg acttcttggt tatcgaacct tctggcaggt taactgaacc atcaagagcc
     3481 agtacatcct tacctgagag ctgcggaata ttcacatcca tattgccgtt tagttggccg
     3541 tgatacatat aaggctggct gctggcctca attttttgtt gcttcacagt atcaatggct
     3601 ttattacgat cttttccgac attctgccag acactgctgt tgttcagatc ggagaagaac
     3661 ggggaatatg aactcgcttt caggatgaaa tattgcttgg tagaactgtc ataatatagg
     3721 tctcccggcg ctcctctccc cccaaaaata ctgacagtat taaccggtac attgatgtca
     3781 ctggctttta atgtctgaag gtcgagtgtt accgttgatt ttttctcact gttattggta
     3841 atgatggccc cgtagtcagc cgcctgaaga cgggtaaagg tcagattatt accattcagt
     3901 tccagacgtc ctccacggta tccccacgaa atattatccg gattaacttg ctgtgagtca
     3961 gaaagcacaa ctgtcggacg accgctggca ataccaacag agctgaaagc ctgtaccttt
     4021 ccgtcagcat ccgcctgctg gttgagaata acggtaccgt cccccacctt gagtccgcct
     4081 gcattcacac cggtcccgtt tactgtcagg gtaccttcgc cagttttatg caggttatca
     4141 ccggcaactc cgttcacctg ccacagaacg tgggttcctt tatcggtgat aatcccgccc
     4201 cccatccatg tctggccgtt cagggcggac accctgtaat cgccggcaaa ctgcagatac
     4261 ccagctccct gattaacgga attctgcaga acgacctcgc cattattgcc ggtgaacagg
     4321 aggttttttc ctgcgttcag atcatttcct ttctttccat gcatatccca agtcgtgttt
     4381 ccctgagtca gtgtgccggt gccattagcc gcatcatatt tccactgcag tactccttca
     4441 ccagaggtgt aggctatagt tttgtcaaaa tcattttgcg gttgctgacc aaggaaatcc
     4501 tgagtggtga cgacccagtt atttccatag ttagttcctg aggaaagcac gccaatcagt
     4561 acccattttt tttgcaggga atcataagca aacagggggg agccgctgtc ccccatattc
     4621 agataattag ccagaggtcc attcgcggga ttaaaaatgt caccggtctg agctgtaatc
     4681 atctgtccgt tgttatagga gttcagcgcg ctgacggttc ccccaacaag aaatccgcca
     4741 ttggtataca cttgggtgcg attaccattt ttgtctttga cgtactgcat ccccccccca
     4801 aggcgataga attcagtaaa gcgtccgccg gcctgatagg caccgctcac agccccgatg
     4861 tcagacactt ctgccggcgc cacctctgtc accagtttac tcaggcggcg ggttttaata
     4921 tcaagaccgc tattattatt tgtaccaacc gcagtatagg tgtttttggc gtatccgaag
     4981 ctcatcgtgt cagaaccgct gacatgtttg gcggtaacca catactgagg ggagtaaagt
     5041 gtatggtctc ccggaggcag gctaccggta gtaatagtgg cactactgaa atcagccatc
     5101 ggggctttat caagtttacc gaccagattg ccctgcttgt cgtaaatgga aatattcgtg
     5161 gtccccggcg taaactgacc tttattctcg gcaaagtcgc ggaatatctg ataagggatc
     5221 tctgcactga ccgttgctga cagagctggc catgccgagg aaagtgataa agctgcaaca
     5281 gacgtaagaa taacccgacg tgaaagtctg cgatggcttt tgcaggtaac cctgcgggcc
     5341 agttcggaga cagcaatcag gcttttggtt atatggcaat acttaagata atatattttg
     5401 ttcatataga aacatcaacc taattttgac aaagattaat tacataggat gtagatggag
     5461 cagtccgaga ttctactaat atcaattatt aagcatctgt tttttagttg gtgtaatata
     5521 atttttatta aggtaagcgg aaaaacaacc aaaaaaaacg caagatgtta tttttttatc
     5581 attttgagcc agtttggctg gggggatact cttctcttaa gcttcttgct caactttaaa
     5641 aatagttatg ccagttcggt atcgataagg ggcagtttgg atatcgagaa ccacggcagc
     5701 ctgacggctg caaattgaaa ac
//
LOCUS       DAJTRB010000181         5703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTRB010000181 DAJTRB010000000
VERSION     DAJTRB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.181"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..170)
                     /locus_tag="OO330_004069"
     CDS             complement(<1..170)
                     /locus_tag="OO330_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884719.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO330_004070"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO330_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5884720.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO330_004071"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO330_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO330_004072"
     CDS             complement(3616..4383)
                     /locus_tag="OO330_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5884721.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="OO330_004073"
     CDS             4579..5487
                     /locus_tag="OO330_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5884722.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            5484..>5703
                     /locus_tag="OO330_004074"
     CDS             5484..>5703
                     /locus_tag="OO330_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5884723.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1411 a         1471 c         1482 g         1339 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gatctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggattgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttattgca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcccaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggca
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 agc
//
LOCUS       DAJTRB010000182         5582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTRB010000182 DAJTRB010000000
VERSION     DAJTRB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.182"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            29..244
                     /locus_tag="OO330_004075"
     CDS             29..244
                     /locus_tag="OO330_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5884724.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            237..623
                     /locus_tag="OO330_004076"
     CDS             237..623
                     /locus_tag="OO330_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5884725.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            631..1317
                     /locus_tag="OO330_004077"
     CDS             631..1317
                     /locus_tag="OO330_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5884726.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="OO330_004078"
     CDS             complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="OO330_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5884727.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2000..3205
                     /gene="tet(B)"
                     /locus_tag="OO330_004079"
     CDS             2000..3205
                     /gene="tet(B)"
                     /locus_tag="OO330_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5884728.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="OO330_004080"
     CDS             complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="OO330_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5884729.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4044..4415
                     /locus_tag="OO330_004081"
     CDS             4044..4415
                     /locus_tag="OO330_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5884730.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4425..>5582)
                     /locus_tag="OO330_004082"
     CDS             complement(4425..>5582)
                     /locus_tag="OO330_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5884731.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1543 a         1030 c         1215 g         1794 t
ORIGIN      
        1 accactatta agtcatgtag ctggttttat ttcaattgaa cgttttcaaa gtctagctac
       61 agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa
      121 aaatcatgtt ttacatgcga aagctcaaca agaagggcga gagtcaatat ttgattttta
      181 caaaattagt attgctcata ttactcgcga atattcattt aaaaaggaca aggataatgt
      241 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
      301 taggtaataa aggtattgga ttggaagggg gagggatatt tacggttggt gatgaatgcc
      361 atgctcattt tcttgttgaa caaggaaagg aagctaaaat agcgctagag caagctggac
      421 tgttagtact tgcgatccgg acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg
      481 aacttggcga aatagcacga gtattggcgg agaataacat taatatttta gtgcaataca
      541 gtgaccatgc taaccaactg atattaataa cggacaatga tagtatggct gcatctgtta
      601 cgctcccttg ggcaataaag tgaacttgcg atggctaatt taatacgaaa agaggttacc
      661 tttgagtcct caatagccgc gataggggcg gctatgtctg acatttcacg agttaaaata
      721 ctcagtgctt tgatggatgg gcgagcttgg acggccactg agctaagttc tgtggcgaat
      781 atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca
      841 gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg
      901 atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca
      961 gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt tgccgttaag
     1021 atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat gatcacatta
     1081 agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca ttcacgtaaa
     1141 atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga
     1201 gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct tggttaccgt
     1261 gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca catttaagtt
     1321 gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt
     1381 ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg
     1441 tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa
     1501 aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata
     1561 aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat
     1621 gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac
     1681 gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta
     1741 acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg
     1801 taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga
     1861 cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca
     1921 ttaattccta atttttgttg acactctatc attgatagag ttattttacc actccctatc
     1981 agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa ttacgttact
     2041 cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac gtgaatttat
     2101 tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg cgttaatgca
     2161 ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc gcccagtgct
     2221 gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt caagtgcgct
     2281 ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg gggctgtcgc
     2341 ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt
     2401 aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt ttgcaggaga
     2461 gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg ctttccttgt
     2521 ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata ccgaagtagg
     2581 ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc ccattttgtt
     2641 gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt gggtgctatt
     2701 taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag cgggtcttgg
     2761 tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta aatggggcga
     2821 aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct ttttagcgtt
     2881 tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg gtgggatcgc
     2941 tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt
     3001 acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat tactgtttgc
     3061 tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta ttggtttagc
     3121 gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc aagctcaggg
     3181 gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat tccgcgaaat
     3241 ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga tttagcttca
     3301 aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg gtaaatagca
     3361 ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc
     3421 ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt aagttggttc
     3481 tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc aaggctaatt
     3541 ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat aaggttttta
     3601 ttattatcaa taatataatc aagataatgt tcaaatatac tttctaaggc agaccaacca
     3661 tttgttaaat cagtttttgt tgtgatgtag gcatcaatca taattaattg ctgcttataa
     3721 caggcactga gtaattgttt tttattttta aagtgatgat aaaaggcacc tttggtcacc
     3781 aacgcttttc ccgagatctc atctattgaa acagcttgat agcctttttc aacaaacaat
     3841 attcgtgctg agttaaccag tgattgatag gtactcttaa aattttcttg ttgatgattt
     3901 ttattttcca tgatagattt aaaataacat accgtcagta tgtttatggt atcatgatga
     3961 tgtggtcgtg acaatcttaa gaacatttag gttattttat gtatattgaa cagcattctc
     4021 gctatcaaaa taaagctaat aacatccaat taagatatga tgataagcag tttcatacaa
     4081 cggttatcaa agatgttcta ttatggattg aacataattt agatcagtct ttactgcttg
     4141 atgatgtggc gaataaagcg ggttatacca agtggtattt tcagcggctg ttcaaaaaag
     4201 taacaggggt cacactggct agctatattc gtgctcgtcg tttgacgaaa gcggctgttg
     4261 agttgaggtt gacgaaaaaa actatccttg agatcgcatt aaaatatcaa tttgattccc
     4321 aacaatcttt tacacgtcga tttaagtaca tttttaaggt tacaccaagt tattatcggc
     4381 gtaataaatt atgggaattg gaggcaatgc actgagagat cccctcataa tttccccaaa
     4441 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
     4501 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
     4561 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
     4621 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
     4681 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
     4741 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
     4801 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
     4861 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
     4921 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
     4981 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
     5041 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
     5101 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
     5161 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga
     5221 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
     5281 ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac
     5341 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
     5401 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
     5461 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
     5521 gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
     5581 aa
//
LOCUS       DAJTRB010000183         5581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTRB010000183 DAJTRB010000000
VERSION     DAJTRB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.183"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..279)
                     /locus_tag="OO330_004083"
     CDS             complement(1..279)
                     /locus_tag="OO330_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884732.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO330_004084"
     CDS             220..405
                     /locus_tag="OO330_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884733.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO330_004085"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO330_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5884734.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO330_004086"
     CDS             complement(1254..1670)
                     /locus_tag="OO330_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884735.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO330_004087"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO330_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO330_004088"
     CDS             1837..2862
                     /locus_tag="OO330_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5884736.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO330_004089"
     tRNA            complement(2915..3004)
                     /locus_tag="OO330_004089"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO330_004090"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO330_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5884737.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO330_004091"
     tRNA            3996..4071
                     /locus_tag="OO330_004091"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="OO330_004092"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="OO330_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1502 a         1324 c         1293 g         1462 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgcc
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 a
//
LOCUS       DAJTRB010000184         5546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTRB010000184 DAJTRB010000000
VERSION     DAJTRB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.184"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..641
                     /locus_tag="OO330_004093"
     CDS             <1..641
                     /locus_tag="OO330_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5884738.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO330_004094"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO330_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5884739.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="OO330_004095"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="OO330_004095"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5884740.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1374 a         1356 c         1539 g         1277 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacag
//
LOCUS       DAJTRB010000185         5402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTRB010000185 DAJTRB010000000
VERSION     DAJTRB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.185"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..497
                     /locus_tag="OO330_004096"
     CDS             <1..497
                     /locus_tag="OO330_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5884741.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2509
                     /gene="cyoB"
                     /locus_tag="OO330_004097"
                     /pseudo
     CDS             519..2509
                     /gene="cyoB"
                     /locus_tag="OO330_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2499..3113
                     /locus_tag="OO330_004098"
     CDS             2499..3113
                     /locus_tag="OO330_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5884742.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3113..3442
                     /locus_tag="OO330_004099"
     CDS             3113..3442
                     /locus_tag="OO330_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5884743.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3454..4344
                     /gene="cyoE"
                     /locus_tag="OO330_004100"
     CDS             3454..4344
                     /gene="cyoE"
                     /locus_tag="OO330_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5884744.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="OO330_004101"
     CDS             4529..4840
                     /locus_tag="OO330_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5884745.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..4982
                     /locus_tag="OO330_004102"
     CDS             4824..4982
                     /locus_tag="OO330_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5884746.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4975..5374
                     /locus_tag="OO330_004103"
                     /pseudo
     CDS             4975..5374
                     /locus_tag="OO330_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1180 a         1343 c         1425 g         1454 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctc tcggtgggcg gtatgactgg cgtgctgctg gcagtaccgg gcgcggactt
     1741 cgttctgcat aacagcctgt tcctgattgc gcacttccat aacgtgatca tcggcggcgt
     1801 ggtcttcggc tgcttcgcag ggatgaccta ctggtggcct aaagcgttcg gtttcaaact
     1861 gaacgaaacc tggggtaaac gcgcgttctg gttctggatc atcggcttct tcgttgcctt
     1921 tatgccgctg tatgcgctgg gcttcatggg catgacccgt cgtttgagcc agcagattga
     1981 cccgcagttc cacaccatgc tgatgattgc agccagcggt gcagtactga ttgcgctggg
     2041 tattctctgc ctcgttattc agatgtacgt ttctattcgc gaccgcgacc agaaccgtga
     2101 cctgactggc gacccgtggg gtggccgtac gctggagtgg gcaacctctt ccccgcctcc
     2161 gttctataac tttgccgtag tgccgcacgt tcacgaacgt gatgcattct gggaaatgaa
     2221 agagaaaggc gaagcgtata aaaagcctga ccactatgaa gaaattcata tgccgaaaaa
     2281 cagcggtgca ggtatcgtca ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg
     2341 gcatatctgg tggctggcga ttgttggctt cgcaggcatg atcatcacct ggatcgtgaa
     2401 aagcttcgac gaggacgtgg attactacgt gccggtggca gaaatcgaaa aactggaaaa
     2461 ccagcatttc gatgagatta ctaaggcagg gctgaaaaat ggcaactgat actttgacgc
     2521 acgcgactgc ccacgcgcac gaacacgggc accacgatgc aggcggaacc aaaatcttcg
     2581 gattttggat ctacctgatg agcgactgca ttctgttctc aatcttgttt gctacctatg
     2641 ccgttctggt gaacggcacc gcaggcggcc cgacaggtaa ggacattttc gaactgccgt
     2701 tcgttctggt tgaaactttc ttgctgttgt tcagctccat cacctacggc atggcggcta
     2761 tcgccatgta caaaaacaac aaaagccagg ttatctcctg gctggcgttg acctggttgt
     2821 ttggtgccgg atttatcggg atggaaatct atgaattcca tcacctgatt gttaacggca
     2881 tggggccgga tcgcagcggc ttcctgtcag cgttctttgc gctggtcggc acgcacggtc
     2941 tgcacgtcac ttctggtctt atctggatgg cggtgctgat ggtgcaaatc gcccgtcgcg
     3001 gcctgaccag cactaaccgt acccgcatca tgtgcctgag cctgttctgg cacttcctgg
     3061 atgtggtttg gatctgtgtg ttcactgttg tttatctgat gggggcgatg taatgagtca
     3121 ttctaccgat cacagcggcg cgtcccatgg cagcgtaaaa acctacatga caggctttat
     3181 cctgtcgatc attctgacgg tgattccgtt ctggatggtg atgacagggg ctgcctctcc
     3241 ggccgtaatt ctgggaacaa tcctggcaat ggcagtggta caggttctgg tgcatctggt
     3301 gtgcttcctg cacatgaata ccaaatcaga tgaaggctgg aacatgacgg cgtttgtctt
     3361 caccgtgcta atcatcgcta tcctggttgt aggctccatc tggattatgt ggaacctcaa
     3421 ctacaacatg atgatgcact aagagcggcg gttatgatgt ttaagcaata cctgcaagta
     3481 acgaaaccag gcatcatctt tggcaacctg atctcggtga ttgggggatt cctgctggcc
     3541 tcaaagggca gcattgatta tcccctgttt atctacacgc tggttggggt gtcactggtt
     3601 gtggcgtcgg gttgtgtgtt taacaactac atcgacaggg atatcgacag aaagatggaa
     3661 aggacgaaga atcgggtgct ggttaaaggc ctgatctctc ctgctgtctc gctggtgtac
     3721 gccaccttgc tgggtattgc tggctttatg ctgctgtggt ttggcgcgaa tccgctggcc
     3781 tgctggctgg gggtgatggg ctttgtggtt tatgtcggcg tctatagcct gtacatgaaa
     3841 cgccactcag tctacggcac attgattggt tcgctctccg gcgctgcgcc gtcggtgatt
     3901 ggctactgtg cagtaaccgg cgagtttgat agcggcgcag cgatcctgct ggctatcttc
     3961 agcctgtggc agatgcctca ctcctatgcc atcgccattt tccgctttaa ggattaccag
     4021 gcggcaaaca ttccggtatt gccagtggta aaaggcattt cggtggcgaa gaatcacatc
     4081 acgctgtata tcatcgcctt tgccgttgcc acgctgatgc tctctcttgg cggttacgct
     4141 gggtataaat atctggtggt cgccgcggcg gttagcgtct ggtggttagg tatggctctg
     4201 cgcggttata aagttgctga tgacagaatc tgggcgcgca agctgttcgg cttctctatc
     4261 atcgccatca ctgccctctc ggtgatgatg tccgttgatt ttatggtacc ggactcgcat
     4321 acgctgctgg ctgctgtgtg gtaacaaaac ctctctatta aaaaggtgct acggcacctt
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt cttggcgtcc ttcagggttc
     5101 acaaccttca gaaattggtg ttgtcagatt agtcatgttg gggggagcgc ggaagtatca
     5161 aaagcggggc tttggtcagg acctactatg tgattttttt gaacatgtaa aaaaaattca
     5221 cccggcatta ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaactttta
     5281 tgctcatctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
     5341 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggcaa
     5401 gt
//
LOCUS       DAJTRB010000186         5387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTRB010000186 DAJTRB010000000
VERSION     DAJTRB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.186"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..945
                     /gene="tauA"
                     /locus_tag="OO330_004104"
     CDS             <1..945
                     /gene="tauA"
                     /locus_tag="OO330_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5884747.1"
                     /translation="NTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESG
                     ASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEAL
                     VVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQ
                     RGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAF
                     AKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAEL
                     TGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            958..1724
                     /gene="tauB"
                     /locus_tag="OO330_004105"
                     /pseudo
     CDS             958..1724
                     /gene="tauB"
                     /locus_tag="OO330_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
     gene            1721..2548
                     /gene="tauC"
                     /locus_tag="OO330_004106"
     CDS             1721..2548
                     /gene="tauC"
                     /locus_tag="OO330_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5884748.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2545..3396
                     /gene="tauD"
                     /locus_tag="OO330_004107"
     CDS             2545..3396
                     /gene="tauD"
                     /locus_tag="OO330_004107"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5884749.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3502..4476)
                     /gene="hemB"
                     /locus_tag="OO330_004108"
     CDS             complement(3502..4476)
                     /gene="hemB"
                     /locus_tag="OO330_004108"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5884750.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5000..5368
                     /locus_tag="OO330_004109"
                     /pseudo
     CDS             5000..5368
                     /locus_tag="OO330_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1253 a         1355 c         1489 g         1290 t
ORIGIN      
        1 aacacacttc ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc
       61 gtggcgtatc aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct
      121 aaagagagcg gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg
      181 gcgctggctt ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca
      241 gccagccaac aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa
      301 gcgctggtgg taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc
      361 gtaccgttta tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt
      421 aaaccagggc tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag
      481 cggggtgata ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac
      541 ggcaaggtgc tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc
      601 tgggtggtgc gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa
      661 agcgccatcg atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg
      721 gaaaacatca gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg
      781 gtgaagggaa atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg
      841 aacaaagcaa tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta
      901 gcgaatgatt acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg
      961 ctgcaaatct ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac
     1021 ctgacgctgg aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc
     1081 accctgctga atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg
     1141 ggtaagcgta ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg
     1201 ctaccgtggc gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag
     1261 aaaatgcagc gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca
     1321 gaaaaacgct atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc
     1381 gcgctggcgg cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc
     1441 ttcacccgcg accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag
     1501 gtgctgttga ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg
     1561 ctttcatccg gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt
     1621 gttgcgggag agtcgagccg cagcatcaag tccgatccac aattcatcgc atgcgcgaat
     1681 atgttttaag ccgcgtattt gagcaacgga aggcgttctc atgagtgtgc tcattaatga
     1741 aaaactgcat tcgcggcggc tgaaatggcg ctggccgctc tcgcgtcagg tgaccttaag
     1801 cattggcacg ttagcggttt tactcactgt atggtggacg gtggcggcgc tgcaactgat
     1861 tagcccgctg tttttgccgc cgccgcaaca agtactggca aaactgctca ccattgccgg
     1921 accgcaggga tttatggacg ccacgctgtg gcagcatctg gcagccagcc tgacgcgcat
     1981 tgtgctggcg ctactcgcgg cggtggtgat cggtattccg gtcgggatcg ccatggggct
     2041 aagcccgacg gtgcgcggca ttctggatcc ggtaatcgaa ctttatcgtc cggtgccacc
     2101 gctggcttat ttgccgctga tggtgatctg gtttggtatt ggtgaaacct cgaagatctt
     2161 actgatctat ttagcgattt ttgcgccggt ggcgatgtcg gcgctggcag gggtgaaaag
     2221 cgcgcagcag gttcgcattc gtgccgccca gtcgctgggt gccagccgtg cgcaggtgct
     2281 gtggtttgtc attttgcccg gcgcgctgcc ggaaatcctc accggattac gcattggtct
     2341 gggtgtgggc tggtctacgc tggtagcggc ggagctgatt gccgcgacac gcggtttagg
     2401 atttatggtt cagtcagcgg gtgaatttct cgcgactgac gtggtactgg cggggatcgc
     2461 ggtgattgcg attatcgcct ttcttttaga actgggtctg cgcgcgttac agcgccgtct
     2521 gacgccctgg catggagaag tacaatgagt gaacgtctga gcattacccc gctggggccg
     2581 tatatcggcg cacaaatttc gggtgccgac ctgacgcgcc cgttaagcga taatcagttt
     2641 gaacagcttt accatgcggt gctgcgccat caggtggtat ttctgcgcga tcaagctatt
     2701 acgccgcagc agcaacgcgc gctggcccag cgttttggcg aactgcatat tcaccctgtt
     2761 taccctcatg ccgaaggggt tgacgagatc atcgtgctgg atacccataa cgataatccg
     2821 ccggataacg acaactggca taccgatgtg acatttattg aaacgccacc cgcaggggcg
     2881 attctggcag ctaaagagtt accttcgacc ggcggtgata cgctctggac cagcggtatt
     2941 gcggcctatg aggcgctctc tgttcccttc cgccagctgc tgagctggct gcgtgcggag
     3001 catgatttcc gtaaatcatt cccggaatac aaataccgca aaaccgagga ggaacatcaa
     3061 cgctggcgcg aggcggtcgc gaaaaatcct ccgttgctac atccggtggt gcgaacgcat
     3121 ccggtgagcg gtaaacaggc gctgtttgtg aatgaaggct ttactacgcg aattgtcgat
     3181 gtcagcgaga aagagagcga agccttgtta ggctttttat tcgcccatat cactaaaccg
     3241 gagtttcagg tgcgttggcg ctggcaaccg aatgatattg cgatttggga taaccgcgtg
     3301 actcagcact atgccaatgc cgattacctg ccacagcggc ggattatgca tcgggcgacg
     3361 atccttgggg ataaaccgtt ttatcgggcg gggtgatacg agtgcggacg gttccctcgc
     3421 ccccttgggg agagggttag ggtgaggggg cgttcactgt actttcaaca ggttaactcc
     3481 ccctttctga gaggaaaaaa attaacgcag aatcttcttc tcagccaaat ccagcgcaaa
     3541 gtagctgaaa atcagatccg cacccgcacg cttaattgaa cctaagcttt cgagcacgac
     3601 tttctcttca tctatagcac ccgccagcgc ggcgaactta atcatcgcgt actcaccgct
     3661 cacctgatac gcgccaatcg gcaattcagt acgttcacgc agctcacgca cgatgtcgag
     3721 gtacgctcca gcaggtttaa ccatcaggca gtctgcgccc tgggcttcat ccagcagtga
     3781 ctcacgaatc gcctcgcgac ggttcattgg gttcatctga tagcttttgc ggtcgccttt
     3841 taatgcgctt cctgcagctt cacggaacgg gccataaaag gaagaggcga acttggtcga
     3901 atacgacata atcgccgtat ctttaaagcc tgcagcgtcc agcgcctgac gaatcgcctg
     3961 tacctggccg tccatcgcgg cggaaggggc gatgaagtct gcgcctgcag cagctgcaac
     4021 cacggcttgc ttgcctaaat tttccagagt cgcgtcgttg tcgacgccat gctcgcacag
     4081 cacaccgcag tgaccgtgag aagtgtattc acagaaacaa gtgtctgaca tgacgatcat
     4141 ttctggcaca gtctgcttgc agatgcgcga catacgcgcc accagtccat cttcccgcca
     4201 ggcgtcgctg ccggtttcat cggtatggtg agagatgcca aaagtcatta cggaacggat
     4261 accggcgttg gcgatgcgtt caatttcgcg tgccagatgt ttctctggaa tgcgcatcac
     4321 gcctggcatg gcttcaacgg ctttgtagtc gtcaatttct tcttcaacaa agatcggcaa
     4381 caccaggtcg ttaaggctaa gtgttgtctc ttcaaacata gcgcgcagcg caggagattt
     4441 gcgcaggcga cgagggcgtt ggattaagtc tgtcatggtc tgcctgatgt ttgtggaatt
     4501 aaggtgcaca gtatacctga agcggggcag gagtgtttta cgaaagttgt cgcgatgttg
     4561 acaagaagag aatgaaagag agaccaggac atctttgtaa tagaaatagc aataatttca
     4621 tattcactga aaatattttt aatctttatt tatagctctt ggttattatt ttttggcgtt
     4681 tggttgtgct gctataaatt gaataattca aactttgttg tatattgata agatatttat
     4741 ctggtttgtg tttttgatat ttataacgcg tatgcatcaa aaataacatt atctgcaaca
     4801 tactgaaata tcgtcctttt atcaatttga tgcgtgtttt tctccataaa ttatatatgt
     4861 ccacattcgg acttagggga aagaataatt gaaccattcg tctgtaacgc agcataatcg
     4921 ttagcgcgaa acataatatg ttttctatgc attgataatt gatggatcaa cttattacgt
     4981 ccctgaggag ggatgacaaa tgcactcctg gaaaaagaaa cttgtagtat cacaattagc
     5041 attggcttgc actctggcta tcacctctca ggctaatgca gcgaccaacg atatttctgg
     5101 tcaaacttac aatactttcc atcactacaa cgacgccacc tatgctgatg acgtttacta
     5161 tgatggttat gtaggctgga acaactatgc cgctgatagc tattacaacg gcgatatcta
     5221 cccggtcatt aataacgcta ccgttaacgg cgtgatttct acctactatc tggacgacgg
     5281 tatttctacc aataccaacg ccaatagtct gacaatcaaa aacagcacta ttcacggtat
     5341 gatttattcc gagtgcatga caactgatta gactggcccc ctgaatc
//
LOCUS       DAJTRB010000187         5336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTRB010000187 DAJTRB010000000
VERSION     DAJTRB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.187"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO330_004110"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO330_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5884751.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO330_004111"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO330_004111"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5884752.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO330_004112"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO330_004112"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5884753.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     AENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO330_004113"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO330_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5884754.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1309 a         1290 c         1455 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatgcg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttc
//
LOCUS       DAJTRB010000188         5143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTRB010000188 DAJTRB010000000
VERSION     DAJTRB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.190"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            2..211
                     /locus_tag="OO330_004114"
     CDS             2..211
                     /locus_tag="OO330_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5884755.1"
                     /translation="MFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(383..712)
                     /locus_tag="OO330_004115"
     CDS             complement(383..712)
                     /locus_tag="OO330_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5884756.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(717..902)
                     /locus_tag="OO330_004116"
     CDS             complement(717..902)
                     /locus_tag="OO330_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5884757.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(899..3538)
                     /locus_tag="OO330_004117"
     CDS             complement(899..3538)
                     /locus_tag="OO330_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5884758.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLSLTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3746..4735
                     /gene="ldhA"
                     /locus_tag="OO330_004118"
     CDS             3746..4735
                     /gene="ldhA"
                     /locus_tag="OO330_004118"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5884759.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4846..>5143
                     /gene="hslJ"
                     /locus_tag="OO330_004119"
     CDS             4846..>5143
                     /gene="hslJ"
                     /locus_tag="OO330_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5884760.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLN"
BASE COUNT         1299 a         1317 c         1202 g         1325 t
ORIGIN      
        1 aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt
       61 ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca
      121 ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct ttgggatgac
      181 gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta ctgcaacgcc
      241 aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga
      301 gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta
      361 tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt atcccttgta
      421 catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat
      481 ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt tcggcattaa
      541 tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa
      601 atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc
      661 tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc atcgcctcag
      721 aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc
      781 tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc agcgacttca
      841 atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag taaaattttc
      901 attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct
      961 gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat
     1021 tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac
     1081 ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg
     1141 accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt
     1201 tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattggcccg ctatttgcca
     1261 gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat gcaccactaa
     1321 atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga
     1381 gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagca
     1441 cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc
     1501 acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt
     1561 cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac
     1621 ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc
     1681 ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa
     1741 cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct
     1801 ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc
     1861 agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct ttaaagagga
     1921 aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc
     1981 cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag gagggatgct
     2041 gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca
     2101 tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt
     2161 catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta aaactccctt
     2221 ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc gccagcccat
     2281 ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac
     2341 gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga
     2401 tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc tcaaaggtca
     2461 gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat aaaatgaggt
     2521 ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc atgcttaatt
     2581 ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg gtcagcacat
     2641 tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg actttgacac
     2701 ccagtcggcc actcaaagga aaacctgcat acagccagct ccagcgacca tcgcttacgg
     2761 tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg
     2821 ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc accagtgacg
     2881 gttcctgcgg caagtttaac gtcgcagtag catgcccact tacgggaagt ccatccggca
     2941 cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga
     3001 catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt
     3061 cgccctgata acgcagttgc tggatatcag aggttaatga gagagagagt tttccctgcc
     3121 attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg
     3181 actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg
     3241 ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg
     3301 aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg
     3361 gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga
     3421 gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc agcagcgtca
     3481 acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg
     3541 gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt
     3601 taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata
     3661 tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag cttaaatgtg
     3721 attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga
     3781 caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt
     3841 tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt
     3901 aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg ttaaatatat
     3961 cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag aactggggct
     4021 gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat
     4081 gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt
     4141 ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac
     4201 cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc
     4261 gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc
     4321 aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta
     4381 ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac
     4441 cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa atcagaaaat
     4501 tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg aagataaatc
     4561 caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt
     4621 taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct
     4681 gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg
     4741 cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct
     4801 attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc
     4861 gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt
     4921 acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg
     4981 accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc
     5041 atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg
     5101 ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atg
//
LOCUS       DAJTRB010000189         5088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTRB010000189 DAJTRB010000000
VERSION     DAJTRB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.191"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            190..447
                     /locus_tag="OO330_004120"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO330_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO330_004121"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO330_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5884761.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO330_004122"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO330_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5884762.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO330_004123"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO330_004123"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5884763.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO330_004124"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO330_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO330_004125"
     CDS             complement(3605..4420)
                     /locus_tag="OO330_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5884764.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5088
                     /locus_tag="OO330_004126"
     CDS             4517..>5088
                     /locus_tag="OO330_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5884765.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVR"
BASE COUNT         1359 a         1160 c         1210 g         1359 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacg
//
LOCUS       DAJTRB010000190         5016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTRB010000190 DAJTRB010000000
VERSION     DAJTRB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.192"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            47..121
                     /gene="tnaC"
                     /locus_tag="OO330_004127"
     CDS             47..121
                     /gene="tnaC"
                     /locus_tag="OO330_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5884766.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            342..1757
                     /gene="tnaA"
                     /locus_tag="OO330_004128"
     CDS             342..1757
                     /gene="tnaA"
                     /locus_tag="OO330_004128"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5884767.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1848..3095
                     /gene="tnaB"
                     /locus_tag="OO330_004129"
                     /pseudo
     CDS             1848..3095
                     /gene="tnaB"
                     /locus_tag="OO330_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
     gene            3227..4402
                     /gene="mdtL"
                     /locus_tag="OO330_004130"
     CDS             3227..4402
                     /gene="mdtL"
                     /locus_tag="OO330_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5884768.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4377..>5016
                     /gene="yidZ"
                     /locus_tag="OO330_004131"
     CDS             4377..>5016
                     /gene="yidZ"
                     /locus_tag="OO330_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5884769.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQS"
BASE COUNT         1143 a         1206 c         1254 g         1413 t
ORIGIN      
        1 ctgagtgtaa taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca
       61 tgtatgtgtg acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta
      121 atttaccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag
      181 tttgctatat cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta
      241 gtaaatgatg gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt
      301 cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc
      361 tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc
      421 gtgaagaagc aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt
      481 ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga
      541 tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag
      601 tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc
      661 aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca
      721 aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc cagattaacg
      781 gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact
      841 ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata
      901 acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac
      961 tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg
     1021 actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag
     1081 actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt
     1141 ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct
     1201 tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat
     1261 atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga
     1321 atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag
     1381 agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac
     1441 tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata
     1501 aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa
     1561 ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat
     1621 atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg
     1681 cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa
     1741 aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc cactctctta
     1801 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag
     1861 ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg
     1921 gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta
     1981 tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt
     2041 taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct
     2101 agaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     2161 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     2221 atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt
     2281 cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga
     2341 tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca
     2401 ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2461 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2521 agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc
     2581 tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg
     2641 gcaacattga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
     2701 tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2761 ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca
     2821 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2881 ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg
     2941 cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     3001 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg
     3061 gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3121 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt
     3181 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3241 gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg
     3301 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3361 cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc
     3421 agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg
     3481 ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc
     3541 aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3601 ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt
     3661 gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat
     3721 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc
     3781 ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct
     3841 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc
     3901 gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc
     3961 gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt
     4021 taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct
     4081 tgccgtttca ccttcccatg cggtttctct gtttggtatc acgctgattt gcgccggttt
     4141 ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg
     4201 cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct
     4261 ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4321 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
     4381 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4441 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt
     4501 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4561 tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga
     4621 gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg
     4681 cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4741 gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta
     4801 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc
     4861 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg
     4921 tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac
     4981 gttatccgca tatcagcatt tgctgggaac agagcg
//
LOCUS       DAJTRB010000191         4919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTRB010000191 DAJTRB010000000
VERSION     DAJTRB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.193"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO330_004132"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OO330_004132"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5884770.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OO330_004133"
     CDS             1020..1703
                     /locus_tag="OO330_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5884771.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OO330_004134"
     CDS             1700..2161
                     /locus_tag="OO330_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5884772.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OO330_004135"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OO330_004135"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5884773.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OO330_004136"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OO330_004136"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5884774.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO330_004137"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OO330_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5884775.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO330_004138"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OO330_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5884776.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1151 a         1293 c         1235 g         1240 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtt cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaa
//
LOCUS       DAJTRB010000192         4916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTRB010000192 DAJTRB010000000
VERSION     DAJTRB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.194"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            235..419
                     /locus_tag="OO330_004139"
                     /pseudo
     CDS             235..419
                     /locus_tag="OO330_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            562..783
                     /locus_tag="OO330_004140"
                     /pseudo
     CDS             562..783
                     /locus_tag="OO330_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(804..866)
                     /locus_tag="OO330_004141"
                     /pseudo
     CDS             complement(804..866)
                     /locus_tag="OO330_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1316..2728
                     /gene="ospC1"
                     /locus_tag="OO330_004142"
     CDS             1316..2728
                     /gene="ospC1"
                     /locus_tag="OO330_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5884777.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2832..2981)
                     /locus_tag="OO330_004143"
                     /pseudo
     CDS             complement(2832..2981)
                     /locus_tag="OO330_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3057..4754
                     /gene="ospD3"
                     /locus_tag="OO330_004144"
     CDS             3057..4754
                     /gene="ospD3"
                     /locus_tag="OO330_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5884778.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLIPDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1691 a          898 c          925 g         1402 t
ORIGIN      
        1 tcccggggcg gttcaatcaa agcgcagaac ttctgcgtct tcttcgtcaa aaaaatcatc
       61 ttcgtgaagc ttcacgacat agcggggaag tttgcttctt tgagaggcgg gtttacgttt
      121 acgggattta gctgaacggg ccatataacc acctgaaaga caatgacatt gcctgttttt
      181 ataacggtaa ttgcagacca tgacaaaccg cagccgtcag gctgcctact cgagtcacaa
      241 ggctctttgt gtccgatatg ccggcagcgc attacccatg aaactggctg gaacgtacac
      301 cataaggtga agaaagttat gggggcggtg aagaattatc taaccttgtc ttgttacatc
      361 caaattgcca tcgacagtta cacagttgtg aagccggctc tctcaaaaag ggcttataaa
      421 ggcttgagcg gtgtaccggg aaaccggtat gcaccgttct taggggaggg cagtccagca
      481 atgaactgtc cttacccgac aaacatccaa aacgaacgga acgtgctgga atccgcatat
      541 aacccgttgt aaagtgagga aatggacacc tcacttgctc atgagaacgc ccgcctgcgg
      601 gcactgttgc agacgcaaca ggacactatc caccagatgg ctgaatacaa ccgcctgctc
      661 tcacagcgga tggcggctta tgcttccgaa atcaaccgac tgaaggcgct ggttgcgaaa
      721 ctgcagcgta tgcagttcgg taaaagctca gaaaaacttc gcgcaaaaac cgaacggcag
      781 attccatttt cacgggcgat ctacgccaca caggctctgg gttgcagtga ctgctccatt
      841 aaggcgattt tgaactcata agggaagtaa caaaaaaact gtaaaagtac aataattata
      901 tatggccaaa ctaacctcta ttaaaaaaca tcagtttttc acacttagtt gagcttcatg
      961 aaaaaatcat cgaggatttc attgcagaat acacattcca ctaactggaa gcactccata
     1021 agaggaatca gagaaagtat cgttttttta tagtaaaatt cattgctgtt caataaaatt
     1081 gatatagtta ttaaattatg aaaataattt cacaaaaaag attaacttga gttcttaagc
     1141 atcggatttt agatattttg aaatatctgg catcacataa catcaaaagt atataatacc
     1201 cttatctaat tacatgcagt atattgtata tatactcaaa aattgtaatt cataccctgc
     1261 tatatcttgt agggaaagat attaattaaa actgttttca tataaggttc attttatgaa
     1321 tatatcagaa acactgaact cagcaaatac ccaatgcaat atagattcta tggataacag
     1381 attacataca ttgtttccaa aagtgacatc agtgcgaaac gctgcacaac aaactatgcc
     1441 agatgaaaaa aatttaaaag atagtgcaaa tattattaaa gatttcttta ggaaaactat
     1501 agcagcacag agttatagta gaatgttctc tcaaggctct aactttaaat ctttaaatat
     1561 agcaattgat gcaccatcag acgctaaagc ctcatttaag gctattgagc accttgacag
     1621 attatcgaag cattatatat ctgaaataag ggaaaaactt catcctcttt ctgcagagga
     1681 actcaatttg ctttcgctaa ttattaattc tgatttaatc ttcagacatc aaagtaattc
     1741 tgatttgtct gataaaattt taaacattaa gtcattcaat aaaattcagt ctgaaggaat
     1801 atgcacaaaa cgaaacacat acgctgatga tataaaaaaa atagctaatc atgactttgt
     1861 gttttttggc gttgaaatct ctaaccatca gaaaaaacac cccctgaata caaaacatca
     1921 cactgttgat tttggtgcaa atgcgtatat cattgatcat gactctccat atggatatat
     1981 gacattaacc gatcactttg ataatgctat tccacctgtt ttttaccatg agcaccaatc
     2041 atttttagat aaattttcag aggttaataa agaagttagt cgatacgtac atggaagtaa
     2101 aggaattata gatgtaccaa tattcaatac taaagatatg aagttagggc tcggattata
     2161 cctgattgac tttattagaa aaagtgaaga ccaaagcttc aaggagtttt gctatggaaa
     2221 aaatcttgcc cctgtggatc tggatagaat cataaacttt gtttttcagc cagagtacca
     2281 tatacctagg atggtaagta cagaaaactt caaaaaagtt aagattagag aaatatcctt
     2341 agaggaggct gttacagcat ctaattacga agaaattaac aagcaggtca ctaacaaaaa
     2401 aattgctctc caggctcttt ttctttcgat tactaatcaa aaagaggatg tcgccttata
     2461 tatattatct aattttgaga taactagaca agatgttatt tccataaagc atgagttgta
     2521 tgatattgag tatctactta gcgctcataa ttcaagctgt aaagtacttg agtattttat
     2581 caataaggga ttggttgatg taaacacaaa gttcaaaaaa actaatagtg gggattgtat
     2641 gttggataac gcaataaaat atgagaatgc agaaatgata aaactattat tgaaatatgg
     2701 tgcaacatct gacaataaat atatttaatc aaaattgaat atcgtttagc aaaaggcagt
     2761 atttgcacaa aatatcgata tattgtttat tgtcagtatg gctcaatgtg ataatagctg
     2821 gaaagtttga tgggtttcgc cccgttgtag cggtagtcga actggaaggt cttcaggcac
     2881 tgcttacagc gatagagcag cccccctgaa ctggaatggc cgttccggat acccccctga
     2941 gtttcagagt aacggtgaca aaccacatca atctttgcca tcaatcatcc aaagggcaaa
     3001 gagtacaaca acactaagtc tgcgtcacaa cccatcaatg aaaggaatat atacatatgc
     3061 catcagtaaa tttaatccca tcaaggaaaa tatgtttgca aaatatgata aataaagaca
     3121 acgtctctgt tgagacaatc cagtctctat tgcactcaaa acaattgcca tatttttctg
     3181 acaagcggag ttttttatta aatctaaatt gccaagttac cgatcactct ggaagactta
     3241 ttgtctgtcg acatttagct tcctactgga tagcacagtt taacaaaagt agtggtcacg
     3301 tggattatca tcactttgct tttccggatg aaattaaaaa ttatgtttca gtgagtgaag
     3361 aagaaaaggc tattaatgtg cctgctatta tttattttgt tgaaaacggt tcatggggag
     3421 atattatttt ttatattttc aatgaaatga tttttcattc cgaaaaaagc agagcactag
     3481 aaataagtac atcaaatcac aatatggcat taggcttgaa gattaaagaa actaaaaatg
     3541 ggggggattt tgtcattcag ctttatgatc ccaaccatac agcaactcat ttacgagcag
     3601 agtttaacaa atttaactta gctaaaataa aaaaactgac tgtagataat tttcttgatg
     3661 aaaaacatca gaaatgttat ggtcttatac ccgacggtat gtctatattt gtggacagac
     3721 atactccaac aagcatgtcc tccataatca gatggcctga taatttactt caccccaaag
     3781 ttatttatca cgcgatgcgt atgggattga ctgagctaat ccaaaaagta acaagagtcg
     3841 tacaactatc tgacctttca gacaatacgt tagaattact tttggcagcc aaaaatgacg
     3901 atggtttgtc aggattgctt ttagctttac aaaatgggca ttcagataca atcttagcat
     3961 acggagaact cttggaaact tctggactta accttgataa aacggtagaa ctactaactg
     4021 cggaaggaat gggaggacga atatcgggtt tatcccaagc acttcaaaat gggcatgcag
     4081 aaactatcaa aacatacgga aggcttctca agaagagagc aataaatatc gaatacaata
     4141 agctgaaaaa tttgctgacc gcttattatt atgatgaagt acacagacag atacccggac
     4201 taatgtttgc tcttcaaaat ggacatgcag atgctatacg cgcatacggt gagctcattc
     4261 ttagcccccc tctcctcaac tcagaggata ttgtaaattt gctggcctca aggagatatg
     4321 acaatgttcc cggacttctg ttagcattga ataatggaca ggctgatgca atcttagctt
     4381 atggtgatat cttgaatgag gcaaaactta acttggataa aaaagcagag ctgttagaag
     4441 cgaaagattc taatggttta tctggattgt ttgtagcctt gcataatgga tgtgtagaaa
     4501 caattattgc ttatgggaaa atacttcaca ctgcagacct tactccacat caggcatcaa
     4561 aattactggc agcagaaggc ccaaatgggg tatctggatt aattatagct tttcaaaata
     4621 ggaattttga ggcaataaaa acttatatgg gaataataaa aaatgaaaat attacacctg
     4681 aagaaatagc agaacacttg gacaaaaaaa atggaagtga ttttctagaa attatgaaga
     4741 atataaaaag ctgaatatta tctcgtggtg atacactgaa tttgaggtaa ccggctcatt
     4801 taaaccgtct ggtctgttac ctccggttcc acaaaaataa tgtccatcat tttgaagtgg
     4861 acactatcgt atggaatacc ggacctggat cactgaagct ttacgccttc atttcg
//
LOCUS       DAJTRB010000193         4821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTRB010000193 DAJTRB010000000
VERSION     DAJTRB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.196"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(275..1132)
                     /gene="sitD"
                     /locus_tag="OO330_004145"
     CDS             complement(275..1132)
                     /gene="sitD"
                     /locus_tag="OO330_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5884779.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1129..1986)
                     /gene="sitC"
                     /locus_tag="OO330_004146"
     CDS             complement(1129..1986)
                     /gene="sitC"
                     /locus_tag="OO330_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5884780.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1983..2810)
                     /gene="sitB"
                     /locus_tag="OO330_004147"
     CDS             complement(1983..2810)
                     /gene="sitB"
                     /locus_tag="OO330_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5884781.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2810..3724)
                     /gene="sitA"
                     /locus_tag="OO330_004148"
     CDS             complement(2810..3724)
                     /gene="sitA"
                     /locus_tag="OO330_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5884782.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4186..>4821)
                     /locus_tag="OO330_004149"
     CDS             complement(4186..>4821)
                     /locus_tag="OO330_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5884783.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1282 a         1174 c         1143 g         1222 t
ORIGIN      
        1 gtcagcggga gctgaaggaa gagctgaata gtctgaagga tttgaattcg gttaggaatt
       61 ttatcgtgga ataacagaag ctgcggcacg tcgtatgcag gaacgtgccg cggttggctg
      121 gtaaactttc gatagtgcga gtattgaatg atttctaggc gttatcgatt ttacgtattt
      181 tttgcatgat agaattcgta cctcctccca acgaccttcc atggctttcc ggacttctgt
      241 aggctggcgg acgatgctgt cctcggatgt tgtgctaatt gggtgataac agatcctgtg
      301 tatgggcgtt tccctttgtg cgtgcgttga tgctggtaac ggtaaacgtc atgatgaaca
      361 cgaccgcgaa taagacgaca atcgtcggag ctggtgcgct gtcgataaaa aaggacagcc
      421 agacaccagt cattgacact attaccgata tgccagtcgc cagtaggagc gcaatatgaa
      481 agcgttgtgt caggagtact gcaatggcgc caggcgcaat gagcagagaa atagagagaa
      541 tgatacctac tgctttcagc gtcgccacaa tggtcaggga aaccatgcac agcagcccat
      601 aatgcagcca tctcgtatgc aaaccgctta cctgcgcctg ttgataatca aagctgaaca
      661 gcaaaaaatc ccgccatttt acgctaataa caagtgtaac cagcccggca ataatcaccg
      721 tctggattat atcgccgatg gttatcccga gcatgtcgcc gaaaagaata tggtcaagat
      781 gtacgtctgg tttaattgca atatacaaga tcagacctgc ggcaaacatg ccagaaaaaa
      841 caatccccat aactgtatct tgcttgattc ggctgttgtc tttcaggtat cccgtcgcca
      901 ccgcgcagaa tactccggcg acgaatgccc ctgtagccag aggtaacccc agaatccagg
      961 taagcacgat gccaggaaaa accgcatgac tcatcgcgtc acccatcagt gcccaacctt
     1021 tcaggaccag aaagaccgac agcagcgcac agggtatgga aactaccagt gaaatcagca
     1081 gtgcgtgact cataaaggtg aactgcagtg gctccagtaa taatgccatc atgagcgatc
     1141 ctccagcgct ttatgcgcac gccgacgatt tgccaaaagt ccatgggtgg gcgcaaatac
     1201 aaatgctaaa agaaacagca gtgtctgcgc caccacgatg attccacctg tcgcgccgtc
     1261 cagaaagtaa ctgacccacg cgccgaggaa actggttaca ctgccaatag ttacagcaat
     1321 tatgagcagg cggggaaaac gatcggttaa caaccatgct gttgcaccgg gagtcaccac
     1381 cagacagatc accagaaagg caccaaccgt ttgcagcgcc gcaacggtcg aaaccgacag
     1441 cagggtgaag aagaggattt ttaatctgcc cggatgtaat ccaatagcac gggcgtgatt
     1501 ctcgtcgaaa aaggtcacca taagatcttt ccatttgaaa aaaagcacga tgatcgagag
     1561 aatgccaatg atagtgagtt gcaggatatc cgccggatcg attgccagaa tattgcccag
     1621 gacgatggtc tgaatgttca cggatgtcgg gttcaatgac accataaaca gccccaggcc
     1681 aaaaaaggac gagaagatca ggccgatgat ggcatcttct tttaagcggg tgcgttggtt
     1741 aagaaacagc atgctgcccg ctgccagtcc gccggaaaag aaggctccca gagaaaacgg
     1801 aagccccagc atatatgccc ccgcaacacc cgggacaata gagtgcgaca gcgcatcgcc
     1861 aatcagcgac cagcctttta gcatcagata gcatgacagg aaagcgcaga gaccgccgac
     1921 cattgccgag acccacatcg cattgagcat atattcatag ctgaagggtt ccagtagcac
     1981 gttcatcttg cttccctctg caccgctgac ggtcgatgtg ccacaaaagg gcgttcatcg
     2041 tcagtaataa tgctttcttc cgagccgtta agtgtgacat ggcgcagtac gccgctaaaa
     2101 gccagctcca ggttctcggc tgtaaaagtg gtgtctgtcg gcccgcttgc caggacggtg
     2161 cctttgacca tgaccgtata atcgcaaaat gtcgtgactg agccgagatt gtgcgttgag
     2221 acaagcatcg ttttgccttc cgcgcgtaac tccctcaaca ggctgatgat tttcgcttcg
     2281 gttttcacat caacgccggt aaaaggttca tccagcagga tcacatcacc ctgctgtgca
     2341 attgctctcg ccagaaagac gcgtttcttt tgtccaccgg aaagctcgcc gatttgacga
     2401 tggcgaaaat ccaccatatc gacacgttcc agcgcatcgg tgacaatctg acgatccctc
     2461 tttttggcta tacgcaacat acccatatgc ccatagcggc ccatcatcac cacatcttcc
     2521 accagtacag gaaatgacca gtcaacctct tctgactgag ggacgtaggc aaccaggttt
     2581 ttctgtagcg cctgtcgcgt gggaataccc agaacagata tctttccgct ggtcagacgc
     2641 acaaatccca taatcgcttt aaacaacgtc gattttccgg aaccgtttac cccaaccaga
     2701 gcggcaatag atccacttgg tactgtgaag gatgcatcgc gcagtgctgt gtgcccgtta
     2761 cgccaggtga cagtaacatc attaacgaca atgcctgcag attgcatcat tatttctccc
     2821 ttttcccggc tttaattccc tgtaccagcg tactggtagt aaccttaaga aggtcgatat
     2881 acgtaggtac cgggccgttt tctgtgctca gggaatcgac atagagcaca ccgccgtagt
     2941 gtgcgccggt ttcacgcgca acctgacgcg ctggtttatc ggaaatcgta ctctcgctaa
     3001 agactgccgg gatatgattt ttcttaacta tatcaacaac cttacgtacc tgctgcggtg
     3061 ttccttgttg atcggcatta atcggccaca gataaagctc ttttagcccc aaatcgcgtg
     3121 cgagataaga aaaagcccct tcactggtga ccatccatcg ctgattctca gggagttccg
     3181 taatctgctt acgcaggggg gcaagggttt gggtaatctt ggctttataa gtatcggcat
     3241 tacgttggta ggtttgtgca tttgccggat cgtattttat caacgcatca cgaatattat
     3301 cgacgtaaat cagagcatta tctggcgaca tccatgcatg gggattaggt ttgccctcat
     3361 agggtccttc ggtgatccct actggcgtca cacccgaaga gacaattact tctggaaccc
     3421 cattgagatg ctggtaaaag cgttggaacc acaattccag attcatacca ttggcgagaa
     3481 tcagttgtgc cccctgcgca cgtttaatat cgccaggggt aggctgatac tcatgaattt
     3541 ctgcaccagg cttggttatg gatgagactt ctgcagcatc tccagccacg tttttggcca
     3601 tatccgcgat gatggtaaat gttgtaatga ccttgaattt ttcagttgca cttgcctgaa
     3661 atgcgatcga gcaggtgagg gcgaggcacc ccaagagcat ggttactttt tttattgagt
     3721 gcataattta gtacctatag tgagcaaaat tagaacaaac atagcaacgg ctatgtttta
     3781 tagcataacc tatacaaaat gaaaataatt atcattaaca gctttgaggc ctaaaacagg
     3841 gattaggttg tttcctgctg gcgacatgat attgaggaaa aaagtagagc agctattagg
     3901 ctttgaaatg actttatgag gaaaattgtt gatggcaaaa atggggcaaa acgctgtata
     3961 aggggcaaaa aaggggcaaa aaagagtggg ttatcgtagc ttattgttgt cgctgataat
     4021 gtttaacata ttgaaaaata aataaaatac ttatgagtca gatagttgtg atttttgccc
     4081 ttacttgttc aggttgtatt gttctttctt tgtaatttgt tgattttctt gcattatttc
     4141 agttctctgg tactaaatgg ggcaaattgg gggcaaactt tgcaactacg ataaccgcgc
     4201 attcaacatg gctatctgtt cgtcgttcat gtcatcaatc cacataccgt aaatttcata
     4261 caccatttgc gcagtttcat gccccatctg gctggctata aatgccgggt tcgctcctgc
     4321 cgtcaacagc cagcaggcaa aagtatgtcg cgtgtggtac ggattacggc ggcgaatacc
     4381 agcacgtttt actgctgcat tccatcttgt acccaaactg cttaccgagt aataaggttt
     4441 ctgtttttcg ttacacaccc tgggcatgaa aacaaaatgc agtttttgtt tttcagttct
     4501 gccgtactcc cgatgataaa aagtgatttc gcttttgcga tgatgcccgg tcagtttgta
     4561 ttgctccttc agtgcttcaa gagccggctg cagtaatgtt accgtccgga tcccggcatt
     4621 tgtttttggg ggaccgaaca tatcaagtat cgtcaggttt cttctgacat tcacaattcc
     4681 cttctcgaaa tccacatcct cccacgccag agctgccagt tccccgtgac gaagcccgga
     4741 gtaaacggca aatttccaca agttttggct ctgtcctttt tcactttcca ttaatgcatt
     4801 gaattctgtt ttagataacg g
//
LOCUS       DAJTRB010000194         4712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTRB010000194 DAJTRB010000000
VERSION     DAJTRB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.197"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..527
                     /locus_tag="OO330_004150"
     CDS             <1..527
                     /locus_tag="OO330_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5884784.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="OO330_004151"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="OO330_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5884785.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3206)
                     /gene="gap"
                     /locus_tag="OO330_004152"
     CDS             complement(2205..3206)
                     /gene="gap"
                     /locus_tag="OO330_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5884786.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3395..3925
                     /gene="cybB"
                     /locus_tag="OO330_004153"
     CDS             3395..3925
                     /gene="cybB"
                     /locus_tag="OO330_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5884787.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4170..4343
                     /locus_tag="OO330_004154"
     CDS             4170..4343
                     /locus_tag="OO330_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884788.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="OO330_004155"
     CDS             complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="OO330_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5884789.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1204 a         1084 c         1187 g         1237 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     2521 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     2581 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     2641 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     2701 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
     2761 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac gttataaacg
     2821 atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc agcatcaaga
     2881 tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat gatttctgcc
     2941 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     3001 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg accatagttt
     3061 gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt aatagcgaca
     3121 acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca
     3181 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     3241 tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc ttacagtgaa
     3301 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     3361 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
     3421 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
     3481 tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg tttcctgtgg
     3541 catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat acccaacccc
     3601 gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg gacatttggt
     3661 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
     3721 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
     3781 ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg
     3841 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
     3901 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     3961 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     4021 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
     4081 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca
     4141 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     4201 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     4261 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
     4321 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     4381 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     4441 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
     4501 gtgtgaatgc cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgcgct
     4561 tcatagcctt tctccttgcc ggatggcggg taagaggcta acaatctgaa ttgcttggtt
     4621 cattcgttgg cctcggtttg tactgacccc aaaaagttgg acagttaaac acgaggcata
     4681 taggtctgat tccgatattc atttggagtc ag
//
LOCUS       DAJTRB010000195         4638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTRB010000195 DAJTRB010000000
VERSION     DAJTRB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.198"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..311
                     /locus_tag="OO330_004156"
     CDS             <1..311
                     /locus_tag="OO330_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5884790.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO330_004157"
     CDS             468..1217
                     /locus_tag="OO330_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5884791.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO330_004158"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO330_004158"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5884792.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO330_004159"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO330_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5884793.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="OO330_004160"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="OO330_004160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5884794.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="OO330_004161"
     CDS             4084..4557
                     /locus_tag="OO330_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5884795.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1239 a         1164 c         1137 g         1098 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctacg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaattc
//
LOCUS       DAJTRB010000196         4617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTRB010000196 DAJTRB010000000
VERSION     DAJTRB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.199"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..227)
                     /locus_tag="OO330_004162"
                     /pseudo
     CDS             complement(1..227)
                     /locus_tag="OO330_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            211..469
                     /locus_tag="OO330_004163"
                     /pseudo
     CDS             211..469
                     /locus_tag="OO330_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(578..1156)
                     /locus_tag="OO330_004164"
     CDS             complement(578..1156)
                     /locus_tag="OO330_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884796.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1577..1814)
                     /locus_tag="OO330_004165"
                     /pseudo
     CDS             complement(1577..1814)
                     /locus_tag="OO330_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2001..2430)
                     /locus_tag="OO330_004166"
                     /pseudo
     CDS             complement(2001..2430)
                     /locus_tag="OO330_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2478..2843)
                     /locus_tag="OO330_004167"
     CDS             complement(2478..2843)
                     /locus_tag="OO330_004167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884797.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGINADG
                     MYCPECGTVHWPDGVIPPF"
     gene            complement(3596..4069)
                     /locus_tag="OO330_004168"
                     /pseudo
     CDS             complement(3596..4069)
                     /locus_tag="OO330_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(3997..4363)
                     /locus_tag="OO330_004169"
                     /pseudo
     CDS             complement(3997..4363)
                     /locus_tag="OO330_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4365..4617)
                     /locus_tag="OO330_004170"
                     /pseudo
     CDS             complement(4365..4617)
                     /locus_tag="OO330_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1123 a         1245 c          950 g         1299 t
ORIGIN      
        1 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
       61 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
      121 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
      181 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
      241 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
      301 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
      361 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
      421 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
      481 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
      541 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
      601 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
      661 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
      721 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
      781 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
      841 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
      901 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
      961 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     1021 tatgacatgc attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt
     1081 tgaactcatc gatagtgtgt tttttattat atatatcatg aaaaatatag cctaaagact
     1141 taaacgagac tttcatttta tattctcctt aaatattaac aggatatata tatcacattt
     1201 tcctctatat tttcagaaac cagctatcat tttccccatt actggtacgt cgcttcagat
     1261 atttccgcag caagcgataa ccctgcgcac tatgaggatg ccattcatta taatgtttga
     1321 acaccttcgc gagactactt tactgctgtt aacacgtcag gttcgaacat aacactgatt
     1381 taatcatatt tcatcgtttt gacgaagttt tctgccattc cattttgcac cgccgtgtgt
     1441 ttgggggcaa ggccgaccat ctgtgtttca ttcgccagat ataagtgatg agatggcatt
     1501 ctccctcctt cacggcagaa gcattttgac cgtttagggg atgcggacga ttttcatgcc
     1561 tgctaagata ctttcgttac tgatgctgtc cgttttccac catttcatca tcactgattg
     1621 gcgttgtgtt tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc
     1681 ctttgatatt cagcgtgtag cagccgatct aggatcgctg ttgccagcac gtgatctccg
     1741 aacatttctc cccagtctgc gacccctttg tttgacgtca gtatgatgct cgctttttca
     1801 tatcgacagt tcatcgatag gaatttaaat ccccaaaaga ttaaaaaagc accgaagaat
     1861 gcgcgtttat tcaacacaac ctgatcatca agtaatcggt atagacactt aatattgctt
     1921 acattgattg attcaatgta tctctgatgg ttttttgctc cgttcccctc aacatggacg
     1981 caacttcccc tctgcgactc tcagccgcac caccgtattc gagccagcgg ctcatgcatc
     2041 ataacctgct ctgccagacg gtaacccctc ttcagccccg taaaccgcat ctgacctccg
     2101 cgcagctcgc acttcagcgg gtcaaccttc agtcactgct gatacatccc tctccttgtg
     2161 atttgcctcg ccgtttttct cactgtctcc gttatggcgt acaccacatc ttccagtaat
     2221 cgccgcttca ccggactcag gaccccgtat caccatccgg aaccccttat ccgccacatg
     2281 ccaggagaac ctttccataa actcgtcgcc actcatcaca aggttttctt ctcgttttgt
     2341 ccggtggctg ttgtaacgca ggccaatttc atcctgaccg gcataatgtt ccaggcggct
     2401 catctgcacc ggctgcttct ccagccagtt aagccatcgg tttccctgct gaaaaatatc
     2461 ggcaaaacgg ggaagcatca gaaaggcgga ataacgccat ccggccagtg aaccgtaccg
     2521 cattcagggc aatacatgcc gtcggcgttg ataccggcag gaattgtcgc aaattcccgc
     2581 tccgtgcagc gggcaatttc cggataccct tcgtcatcaa cacgtacaaa ccagaagacc
     2641 agttttttgt ttccggcatc cacaaagaac ggaatattca ggtctgcgca acactcaacg
     2701 gcatcgtcaa aattatcaaa gcgcagaacc tcagcttctt cttcgtcaaa aaaatcatct
     2761 tcgtgaagtt ttacgacata gcggaggagt ttgcttcttt gaggtgcagg ttttcgtttg
     2821 cgtggtttag ctgaacgggc catataacta cctgaaagac aatgatattg tctgttttta
     2881 taacggtaat cacaggccat gacaagccgc agccgtcagg ccgcctactc ggggcggttt
     2941 agaacgctta tttagtacca gatcttactt ataataaagg ggcttggcag tttggataaa
     3001 ctattgtcct tattccacta atacaaactt tttgtaaagt atattaaata tacttcattt
     3061 catactgaaa tccccggaag tattcaatca aacaattaca ccataaaagg aagagggggc
     3121 cccaattaag ttgtcaagca tgtttactgc gcaatcgctt ggtgctccgt ccctgtaccc
     3181 cgcattggta ctctggcgct catggggctt ccacttgcgc ttctcccttc acatcggggt
     3241 gacaggtcca cgttccccac aagagcctga atcgacttca cgccgctttt atgccggatg
     3301 ccgcgcaggc agtaaacata tcccacagga acgactccac cattgttttg acatcatcta
     3361 tatgctttcg acacatcatc agcggttcac tttcgttcgt ctcttcgaac catacctgat
     3421 cggctcacgt ccgacctttt cccatctcgc tcaccaccac atctcttaaa tgcagcagca
     3481 gtggatggtt tggtgcctgc gcctgcacgc cgacaccaag gggccttccc tcatctcttg
     3541 tgctgcatgg ctgcacaggg actcacgtcc ccaggacgcg ccttcgtggc acacagtcat
     3601 cggcatagca acagtaagca acggcaggtt tccattgcca gttttctttg acagcagtac
     3661 ttcggcctcg ctggatactg tgattccagt accagcgatc ctttctggct tttccactca
     3721 ggtagcgttt attcagatac tggtcgaact cattcagtat gatgttcgac agcaacggcg
     3781 agatcacacc gccctgtggc atactttcac tgaccgcccg gaagagacca acatcaacac
     3841 ggcgttttcc acagcagtct cataaaacgt gtatcactga tcctgcggca gacggctttc
     3901 atcagcagcc ggtgatgtac tgtgtcgaag tagctggaca ggtcaccttc aataacccgg
     3961 cgccccgtgt ttccccgcag tcggtgagct gtaatagtac gtgatgtctc ccgcccactt
     4021 ctggttcggg ccactggcgt aaaaatcctg cgtcagcaga ttttctgata caggcaggcc
     4081 gtgtgcacgg taactgatcg ggctgaactt ctgggaggct tttgcgctca gttcctgacg
     4141 gcgcagactg gctgccacgg tttttacgtt aaagtggtaa ccagagcaca cagttcatcc
     4201 gtcaggcgtg gggcaccgta acgctggact gggtaaaagc cttgcggaca acgctgtcgc
     4261 agtgctgacg gaactgctga cgtgtgttta tccttgtccg ccgctgacac cacgtatacc
     4321 agccgctgcg ggccacccgg agtacgcggc acattgcctt gatgacggtt tacgcggtcg
     4381 cctgcggcaa cggctttccg gctgacggta gtgtggacct ttttaccccg gagaacaccg
     4441 gcaagcccca taaccgccat gagacgtgcc acagtgcatc tggccactct gataccttcc
     4501 cgtaacaact gatgccagac tttacgcaca gcgtatacct gatgattctc atcgtatacg
     4561 cgcagtatct ctttcttcag ccagtcatcg cgctgcgcac gggcactgcg tttatca
//
LOCUS       DAJTRB010000197         4566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTRB010000197 DAJTRB010000000
VERSION     DAJTRB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.200"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..246
                     /locus_tag="OO330_004171"
     CDS             <1..246
                     /locus_tag="OO330_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5884798.1"
                     /translation="ATTDSSRQVFANTAPDATKASGVSVTLMRNGKILATGENVSLGT
                     VNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            580..2859
                     /gene="ydeP"
                     /locus_tag="OO330_004172"
     CDS             580..2859
                     /gene="ydeP"
                     /locus_tag="OO330_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5884799.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3107..3310
                     /gene="safA"
                     /locus_tag="OO330_004173"
     CDS             3107..3310
                     /gene="safA"
                     /locus_tag="OO330_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5884800.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3378..4139
                     /gene="ydeO"
                     /locus_tag="OO330_004174"
     CDS             3378..4139
                     /gene="ydeO"
                     /locus_tag="OO330_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5884801.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1313 a          971 c         1014 g         1268 t
ORIGIN      
        1 gccaccactg acagtagccg acaagtgttt gctaacacag cacctgatgc aacaaaagca
       61 tcgggagtga gtgtcactct aatgcgtaat ggaaaaatac tggctacggg agaaaatgtc
      121 tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac ctatggacaa
      181 acaggaaaca aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac ttttatctat
      241 gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg cgcggataat
      301 tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat tgaagccaga
      361 gtcttatttt ttatattgaa aaatatgtta atttaaacac tatgagagat gatgcttaac
      421 ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga gagtagtccc
      481 ttactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca tcatcgctat
      541 tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa aattgaatcc
      601 taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc agtacgtaag
      661 cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc agagggcttt
      721 gactgtccgg gttgtgcatg gccagatcct aagcacagtg cgtcattcga catttgtgaa
      781 aacggcgcaa aagcaatcgc ctgggaagtc acggataagc aggtaaatgc ctctttcttt
      841 gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga ggctgcgggg
      901 cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc attaagctgg
      961 caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc caatcaggtt
     1021 gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca gctttttgcc
     1081 cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga accgacaagc
     1141 gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga agactttgag
     1201 aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgctg
     1261 acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctctacag
     1321 gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct
     1381 gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat ggcgttgctc
     1441 aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc aggtcggccc
     1501 tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga gctacgccgt
     1561 gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa
     1621 atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg ttacggaatg
     1681 gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa tttgctgttg
     1741 atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg tcactctaat
     1801 gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc
     1861 ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc aattgccagc
     1921 atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg caactttgca
     1981 ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga tttggcggta
     2041 cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg
     2101 ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc ggtaaccgtt
     2161 gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg
     2221 ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg
     2281 gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg
     2341 ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt tcacctgata
     2401 aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattaccagc
     2461 aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat ggcgacagta
     2521 cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggggta
     2581 ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa
     2641 aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag ctcacgccgc
     2701 atggatagat taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt
     2761 ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg cattcctggc
     2821 tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc atttcttctg
     2881 ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt tgctcacatg
     2941 gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt aagaaactcc
     3001 gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat attaagcata
     3061 ctgattaacg acttttaacg ttatccgcta aataaacata tttgaaatgc atgcgaccac
     3121 agtgaaaaac aaaatcacgc aaagagataa ctataaagaa atcatgtctg caattgtggt
     3181 tgtcttatta ctgacactta cgttgatagc cattttttcg gcaattgatc agctgagtat
     3241 ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata gtttgcaaga
     3301 ctggaaataa acagcaaatt ataaatgtga aatgtttaaa aggtatcgat aaaactttat
     3361 tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat catgctttca
     3421 acgctaacat tttagataaa gattacgcct tctccgacgg cgagatcctg atggtagata
     3481 acgctgttcg tacacatttt gaaccttatg agcggcattt taaagaaatc ggatttaatg
     3541 aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg gtgcctgttc
     3601 ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa atgattgctt
     3661 atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt ttttcttgcc
     3721 tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt gtgctatccg
     3781 tttccgggaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg aagctgaaag
     3841 atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc
     3901 aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa aatttgatac
     3961 gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt acatcttatt
     4021 ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct aaggagtacc
     4081 gttgtcaaag gcacacgggt atgaatacgg gcaacacgat gagtgcttta gctatttgat
     4141 tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg gcagccattc
     4201 cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga catccatcac
     4261 atatttatgc acttgcataa cctgttgcat gattatttat gatctcaatt cggcattttg
     4321 tcagtaaaat gcaataattt attaaatatc aataaattag ttgtttatcg gcgagaaatt
     4381 acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc tttttttagc
     4441 taaatctgct atctctttat gtgactaact tcacttacat ccacttattt ctcttcgtaa
     4501 aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa
     4561 aagtgt
//
LOCUS       DAJTRB010000198         4550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTRB010000198 DAJTRB010000000
VERSION     DAJTRB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.201"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..1224
                     /locus_tag="OO330_004175"
     CDS             <1..1224
                     /locus_tag="OO330_004175"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5884802.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="OO330_004176"
     CDS             1224..1898
                     /locus_tag="OO330_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5884803.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2426..3187
                     /locus_tag="OO330_004177"
     CDS             2426..3187
                     /locus_tag="OO330_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5884804.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="OO330_004178"
     CDS             3231..4310
                     /locus_tag="OO330_004178"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5884805.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="OO330_004179"
     CDS             4307..>4550
                     /locus_tag="OO330_004179"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5884806.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          863 c         1119 g         1319 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagtatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DAJTRB010000199         4233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTRB010000199 DAJTRB010000000
VERSION     DAJTRB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.203"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..158)
                     /locus_tag="OO330_004180"
     CDS             complement(<1..158)
                     /locus_tag="OO330_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5884807.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="OO330_004181"
     CDS             complement(308..469)
                     /locus_tag="OO330_004181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884808.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="OO330_004182"
     CDS             complement(466..654)
                     /locus_tag="OO330_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5884809.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="OO330_004183"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="OO330_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5884810.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="OO330_004184"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="OO330_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="OO330_004185"
     tRNA            complement(2161..2237)
                     /locus_tag="OO330_004185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="OO330_004186"
     tRNA            complement(2339..2414)
                     /locus_tag="OO330_004186"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="OO330_004187"
     CDS             complement(2559..3380)
                     /locus_tag="OO330_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5884811.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="OO330_004188"
     CDS             complement(3395..3751)
                     /locus_tag="OO330_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5884812.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4233)
                     /locus_tag="OO330_004189"
     CDS             complement(3764..>4233)
                     /locus_tag="OO330_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5884813.1"
                     /translation="IRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPQQEK
                     TVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1168 a         1144 c          835 g         1086 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatca
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaagacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgggttctg ttgctggtgg gcgtgaggca
     4141 gcggcgcaag attttttgtg cgctgtttca gtatgctggt ggcggtctgc tctcccggta
     4201 cgatgtcgct ttcacggtac attgagcgga ttt
//
LOCUS       DAJTRB010000200         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTRB010000200 DAJTRB010000000
VERSION     DAJTRB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.204"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO330_004190"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO330_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5884814.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO330_004191"
     tRNA            complement(324..399)
                     /locus_tag="OO330_004191"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO330_004192"
     tRNA            complement(406..480)
                     /locus_tag="OO330_004192"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO330_004193"
     tRNA            complement(597..681)
                     /locus_tag="OO330_004193"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO330_004194"
     tRNA            complement(690..765)
                     /locus_tag="OO330_004194"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO330_004195"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO330_004195"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5884815.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO330_004196"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO330_004196"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5884816.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO330_004197"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO330_004197"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5884817.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1047 c          937 g         1136 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgc gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTRB010000201         4199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTRB010000201 DAJTRB010000000
VERSION     DAJTRB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.205"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            84..752
                     /locus_tag="OO330_004198"
     CDS             84..752
                     /locus_tag="OO330_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884818.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="OO330_004199"
     CDS             complement(995..1690)
                     /locus_tag="OO330_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5884819.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="OO330_004200"
     CDS             complement(1683..3110)
                     /locus_tag="OO330_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5884820.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="OO330_004201"
     CDS             complement(3121..3840)
                     /locus_tag="OO330_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5884821.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1120 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcggaa
//
LOCUS       DAJTRB010000202         4168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTRB010000202 DAJTRB010000000
VERSION     DAJTRB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.206"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO330_004202"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OO330_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5884822.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO330_004203"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OO330_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO330_004204"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OO330_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3787..>4168
                     /locus_tag="OO330_004205"
     CDS             3787..>4168
                     /locus_tag="OO330_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5884823.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1206 c         1023 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agcagccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacgtag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAJTRB010000203         4159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTRB010000203 DAJTRB010000000
VERSION     DAJTRB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.207"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..439
                     /gene="tnpA"
                     /locus_tag="OO330_004206"
                     /pseudo
     CDS             1..439
                     /gene="tnpA"
                     /locus_tag="OO330_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(557..1501)
                     /gene="hypT"
                     /locus_tag="OO330_004207"
                     /gene_synonym="qseD"
     CDS             complement(557..1501)
                     /gene="hypT"
                     /locus_tag="OO330_004207"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5884824.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1846..2565
                     /locus_tag="OO330_004208"
     CDS             1846..2565
                     /locus_tag="OO330_004208"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5884825.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2631..3929
                     /locus_tag="OO330_004209"
     CDS             2631..3929
                     /locus_tag="OO330_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5884826.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1050 a          904 c         1027 g         1178 t
ORIGIN      
        1 gcgcacaccc gatggaactg taaatatcac atagtttttt gcgccaaaat accgaagaca
       61 ggtgttctac agagagaagc gtagagcaac aggcagtatt ttgagaaagc tgtgtgagtg
      121 gaaaagcgta cggattctgg aagctgaatg ctgtgcagat catatccata tgcttgtgga
      181 gatcccgccc aaaatgagcg tatccggctt tatgggatat ctgaaaggga aaagcagtct
      241 gatgctttac gagcagtttg gtgatttgaa attcaaatac aggaacaggg agttctggtg
      301 caggggggac tacgtcgata cagtgggtaa gaacacggcg aagatacagg attacataaa
      361 gcaccagctt gaagaggata aaatgggaga gcagttatcg attccctgtc cgggcagccc
      421 gtttacggac cgtaagtaac gaagttggat gcaaatgtca gatcgtgtgc gcctgttagg
      481 gcgcggctgg taagagagcc ttataggcgc atttgaaaaa cctccggcta tgccggagga
      541 tatttattgc acgtcgtcaa ttgcggcgtg ctggctcttc cattgatatc tgctggatta
      601 aatcgtctgg catatgttcc tgtaaaatac gccagaggtt ctcggcggtt ctattcagtc
      661 gggtgttcat tcggtaaata taagcctgaa tggggactgt caatttagca gcatcaaggc
      721 atatcagacg tttggtctgt agttcatcga cgatcgacca gatcggtaac caggcaatgc
      781 cgtatccgtt gagcgccata ttctttaata actcgctcat cgaagagata aatatcgtgc
      841 gcccggtaat acctccaacc tctgccagat gacgattgac caacctgccc atataagatg
      901 tggcggtata gttgagcaaa ggaacctggg gctgagtgat atcgaataca gggtgcccct
      961 gtgcgtcggc ggcacatacc gggtacaact cagattcaaa cagtttcagg cagcaaaaag
     1021 gcgattgcat aagatcttca tcgcgaaacg aaataataaa gtcactcttg ccttcgcgta
     1081 acgtatttac ggcctggaca acatcaatgg cttcaacatg atagacaaat tctccgccat
     1141 aggcggtaag tgaatgcacc agtttgggta atacgctcag ggatagtgag tgcgcggctg
     1201 caattttaat gttgggaacg ccgagtaaac tctggccgtt taactccccc aaattgcatt
     1261 caagctgctg taataagctg cgagtctggg aatgaaagag cttgccctct tcggttaatt
     1321 gtaaaggggt ggtcgtgcga tcgaataaaa caacgccgat cgctgcctcc agcgccttaa
     1381 ttcgtcggct gaatgcgggt tgcgaaagat tacgctcttt tgccgcctga gaaaaatggc
     1441 gacaggcttc cagtgtcagg aaatcgtata accattttgt ctcgatattc ttcattacca
     1501 tggcacctgt attttcaatc caaagacata ataaaacatc ccggcaataa aaataatgtt
     1561 ctcaattagc acaacctggc ctttatcatt cgttttgtgc ataacccctt catttccggt
     1621 attgacgttg atatcacatt tcgccaaaaa aacgaaattt ttccgtcaga taaccagaat
     1681 acatcatcac tatggtgaat aagcggaatg ttccatagct gcatcatgcg tgtcttgcat
     1741 cgccttgtac aaccttgcat ttctcatcgc ccatttcgac tgttaaaaaa gagagcagca
     1801 gttatttacc gcgtattgtc attaacgttt tgagaggcag gtattgtgaa aggtttaatt
     1861 ggtgtactcg gtggaatggg gccggcagct accgtcgacc tgtttaataa atttgtaact
     1921 tttaccgctg cacaatgtga tcaggaacat attcctttaa ttatttcttc gatcccggat
     1981 attcctgacc gtaccgatgc attaatgcat catgggcgtt caccattacc cgttatgcgt
     2041 gattatatgc ataagctgga agatgccggg gcagaatgta ttgttattcc ctgtaatacc
     2101 gcgcattact ggttcaaaga attaaaagat gcttgtcata ttgatatatt aagtatcgta
     2161 gaaactacga tcaatgaagt tcgcgcctgt ggtaaaaccc gaattggctt gttagccacc
     2221 aatgccacgc tatatatggg tttataccaa aaaggaattg agtcgctggg atttacctgt
     2281 gtcagccccg atgccgacgg tcagaaaaaa gtgatggaaa gtatttattc cttgaaagct
     2341 ggcaatatag cgcacgcaca aaagttgatg aatgagcagg ctgaaatatt attttcccgc
     2401 ggtgcagaaa tccttgttct tggttgtaca gaaattccgg ttattctggc gcaagccgtt
     2461 aaagagcagc cttcccgcta tattgactca acggcgtcac tcgttcgtgc cggaataaaa
     2521 tggtacgaaa atcgtgtcgg taaacattat cttttaaccc aataatcatt taaatcgcag
     2581 ccagaaagtt attcgctttt aactgaatta tatttataac ggagaacatt atggtttggc
     2641 tggaaattat cgtagtactt ggtgcaatat tttttggtat tcgccagggg ggaatcggta
     2701 ttggtttatg tggcgggctt gggcttgcca ttctgactct gggatttggt ctgcctatgg
     2761 ggtcgccgcc agtcgacgtg atcctgatta ttatgaccgt tgttgtggcg gcctcagcct
     2821 tacaggccgc aggcggaatg gattatctgg tacgcgtcgc tggtaatttt atgcggcgta
     2881 acccgaaata tatcaacata atcgcgccga ttatcacctg gtctatgacc attatggccg
     2941 gtactgggtt tattgttttt tccacgctgc cggtgattgc tgaagtggcg aaagagtccg
     3001 gtatccgtcc ttcccgcacg cttgccggtt cggttgtcgc ttcgcaggtt gcgatttccg
     3061 gttcgccgat cagcgccgca atggcggcta tgctcactat tatggaggta aacggtgtca
     3121 gttttattca agtgatgtcg gtatgtcttc ccacctcttt tgctgccgcg atggtcgccg
     3181 cttttattgc ttcccgacag ggatgtgagt tgcaggatga cgaggtctac cttgagcgcc
     3241 tgcaaaaagg actggtgcaa aaatacgaga ataatagcag catcaagcca ggtgcgacgc
     3301 tctccgtcgg attgttcctg ctggcgacgg tcgctattgt tattcttgct gccttccccc
     3361 aactgcgtcc gggttttgat atcggtaaac cgatggaaac acgcgatatc attattatct
     3421 gtatgctctc tgccgcctgc ctgatggtgg tgctgtgtaa gacggcgacc gatgccatta
     3481 ttctcacctc cacattccgt gcggggatga gttcgctggc ggtaattctc ggcattgtaa
     3541 ccttaggcac cacttttatt gatgcgcatt taactgagat taaagatatt gctggtgata
     3601 ttttgcaggc gtatccgatg ttgttggcgc tggttctttt tggcacttgc gcgctgctct
     3661 actcgcaagg tgcgaccacg ccgttaatta tcccgcttgc cgtcgcgctt gacgtgccca
     3721 cctgggcaat tctggcgtcc tatgtggccg tcaccggagt atttgtactg ccaacgtatc
     3781 caacatcact ggctgccatg gaatttgata ctaccggcac aactcgtgta ggcaaatatg
     3841 tgctgaatca tccatttatg ctgcctgggt tggggggaat tattgctgga gtggcattag
     3901 gctttgtgat tgcgccaatg attatttagc actatcaaac atcagtatcg atggtttata
     3961 cacaataaag acagatatgg cggaatatag tctgttgtaa gcgccaacaa agaatagggg
     4021 aattccgcca tgtctgaaca tcttcacaat atggtattaa gtgttgtcgt ttaatgattt
     4081 atttcttggt tatgttgtgt attagctgca tctatcgctt tttgatacct gaggtgtact
     4141 ggcaatagcg gacactacc
//
LOCUS       DAJTRB010000204         4113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTRB010000204 DAJTRB010000000
VERSION     DAJTRB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.208"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..224
                     /locus_tag="OO330_004210"
     CDS             <1..224
                     /locus_tag="OO330_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5884827.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OO330_004211"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OO330_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5884828.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OO330_004212"
     CDS             518..1108
                     /locus_tag="OO330_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5884829.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OO330_004213"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OO330_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5884830.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OO330_004214"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OO330_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5884831.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OO330_004215"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OO330_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5884832.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3456..3647
                     /locus_tag="OO330_004216"
     CDS             3456..3647
                     /locus_tag="OO330_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884833.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3877..>4113)
                     /locus_tag="OO330_004217"
     CDS             complement(3877..>4113)
                     /locus_tag="OO330_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5884834.1"
                     /translation="SPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCV
                     LCGSQMRFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         1097 a         1035 c         1015 g          966 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc tggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa aaaaacctgt tcactgcctg tcctgtggac agacagatat
     3121 gcacctccca ccgcaagcgg cgggccccaa ccgaagccgg tttagttaca acatgcaaac
     3181 ataaacccct aaaaaaccca agtccaacgc agaactgaaa ccacaacgcc cccctcataa
     3241 ctgaaaagcg ccgccgcccc cgcccaaagg gcgggaacag agtcgctttt aattatgaat
     3301 gttgtaacta catcatcatc gctgtcagtc ttctggctgg acgtatcgag tacacgctcg
     3361 taagcggccc tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaaccctcg
     3421 tcgggaccac tccgaccgcg cacagaagct ctatcatggc tgaaaacggg tatggcttgg
     3481 cagggctggg gatgggtaag gtgaaatctg tcaatcagta ccggctgacg ccgggcttcg
     3541 gcggttttac tccggtatca catgtaacca cagcgtgccg ccttccatgc cgctggcgcg
     3601 gcatcagaat aatacaagca gcatttaatg catttgcaaa agtgtgagta atattaagtg
     3661 gtcgtttcag ggatatcgtt aagacttgag cgataggaat taaaacccca aaaagattaa
     3721 aaaaacacca caaaacggat gtttcttcaa caccactttt gctccatatg aacggaaccg
     3781 acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt ttgctccgtt
     3841 tccctcaaaa tggacgcaac ttcccctctg cggctctcag ccgcaccacc gcatccgggc
     3901 cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca gccccgtaaa
     3961 acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta acctctgata
     4021 catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta tgatgtacac
     4081 cacttcttcc agtaaccgcc gtttcgccgg act
//
LOCUS       DAJTRB010000205         4059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTRB010000205 DAJTRB010000000
VERSION     DAJTRB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.209"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(903..1094)
                     /locus_tag="OO330_004218"
     CDS             complement(903..1094)
                     /locus_tag="OO330_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5884835.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1158..1481
                     /locus_tag="OO330_004219"
     CDS             1158..1481
                     /locus_tag="OO330_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5884836.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1471..1782
                     /locus_tag="OO330_004220"
     CDS             1471..1782
                     /locus_tag="OO330_004220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884837.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1757..2275
                     /locus_tag="OO330_004221"
     CDS             1757..2275
                     /locus_tag="OO330_004221"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR5884838.1"
                     /translation="MHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLV
                     NHHYGLHDPNAPENRRTLTLPDNLPETKQAIAEGVTRGIDALYHAGEIKGRQDVIQAL
                     TEAGIDVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGCGVREKAERESRIYRENA
                     ERRVQEARRICK"
     gene            2160..2690
                     /locus_tag="OO330_004222"
     CDS             2160..2690
                     /locus_tag="OO330_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5884839.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2697..2930
                     /locus_tag="OO330_004223"
     CDS             2697..2930
                     /locus_tag="OO330_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5884840.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3476..3943)
                     /locus_tag="OO330_004224"
     CDS             complement(3476..3943)
                     /locus_tag="OO330_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884841.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          992 a          923 c         1178 g          966 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgccgg gagcagcata
      901 tgtcagaggt tttcaccgtc atccccgaaa cgtgcgaggc agctgcggta aagctcatcg
      961 gcgtggtcgt gaaacgattc acagatatca gcctgttcat ccgcgtccag ttcgttgagc
     1021 ttctccagca gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     1081 tcctgtttag tcactgatgc ctccgtgtaa gggggatttc tgttcaggag gtaatgatac
     1141 cgatgaaacg cgagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     1201 tggaacgctg tgagggtaaa cagctggcgg tatggatgcg gcgggtctgc ctgggggagc
     1261 cggttgcccg ttccgggaaa ctgccgacac tggcaccgcc gttactgtgt cagctggctg
     1321 ccatcgggaa taacctgaat cagacagcga ggaaggtgaa cagcgggcag tggtcttccg
     1381 gtaaccgggt tcaggtggtg gccgcactga tggccatcgg ggacgagctg cgccggctgc
     1441 gtctggccgt cagggaacag gggacgcggg atgatagtta agtttcatgc ccggggaaaa
     1501 ggtggtggca gtggtccggt tgattacctg ctgggaaggg agcgtaaccg ggaaggggca
     1561 acggtgcttc agggtaatcc ggaagaagtc cgggaactca tcgatgccac gccatttgcg
     1621 aagaaataca cgtccggtgt tctgtcgttc gcggagaagg agctgccgcc gggaggacgt
     1681 gaaaaagtga tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac
     1741 agcatcctgt gggtggatgc accaggacaa gggacggctt gagctgaatt ttgtcattcc
     1801 gaacatggag ctacagaccg gaaaacgcct ccagccgtac tacgaccgcg cagacaggcc
     1861 tagaattgat gcctggcaga cgctggttaa tcaccattac gggctgcatg acccgaacgc
     1921 cccggagaac cgcaggacgc tgacactccc tgataatctg ccggaaacga aacaggcgat
     1981 tgctgagggc gttacacgcg ggatagacgc gctttaccat gccggagaga taaaaggccg
     2041 tcaggatgtg attcaggctc tcacagaggc agggattgac gtggttaggg tgacgcgaag
     2101 cagtatcagc attgcagacc cgaacggcgg gaagaatatc agactgaaag gagcgtttta
     2161 tgagcaatct tttacagacg ggtgcggagt tcgagaaaaa gctgaaagag agagccgaat
     2221 ctacagagaa aatgctgaac gacgagttca ggaagctcga agaatctgta aatagggaac
     2281 tgacctcaaa cgaatcttta atcaggaacg ctatcaacga ccatacatca gccctgaaag
     2341 aacttctgga gaggtatcag aaaacgaccg tcgatacgat ggacgcacac tggaaaaccg
     2401 tactgaaaat gagcgtgaaa cgctggctgt ggctgattat cgtctcagta ctgatgtttg
     2461 ccacgaccgg gagcctgctg tggtaccagg gcatgaagat aaacgcgaac atgaatattc
     2521 tccgcgagca gaaagagagt ctggagaagc tgaacgcgaa gacatggggc gtgcgttatc
     2581 acgaggacag caacggacgt tttctggtgt tgcccgaggg gatgaaagcc gaaacgaact
     2641 ggacgatgga aaacgggaaa cagaatgccg taaggctggt acaggagtaa cagagcatga
     2701 cggagctgga aagacatttg ctgaacgcat tagaacaact gcaacaggac tatatgcagc
     2761 ggctgaacga atgggagagc gccttcgtgg aattgcagaa aatgttttcg cttacgcaac
     2821 gggacaacgc gatgctgaac gagcgggtca tgcagttgag tcagcaggtg cagcacttga
     2881 gcgagcagac agaacgcttg agccagttat acagcgagaa caggagataa gggacgaacg
     2941 gctgatacag gaatgcgaac atgcgttatc cctggagcgt gagcaccagc tggaaataca
     3001 ggaacggaca ctggatggcc cttcgctggg atggtaatca gagacaggct aaaatgttgt
     3061 cagcgcgttt ccggtgcatt ttttgggata atggttggtt cgggggcacc gggaacgcgc
     3121 ttcagtcaga ccttgctgaa aacataggaa cgcgttaaaa cgcatacggg cgcgattctg
     3181 gtgtgtcttt tgttgctgtg ttttgcctct attgccagat cgcgctcatt cccctgaact
     3241 tcttttttcc cgctcaggtc cccgtcttct tcgcttccga aagcatgaaa aaaatccgtt
     3301 tcagctgtct ctggctggca attcctgcgt gattcacatg gctgcatagc tatgcatgtg
     3361 gttaaaattt accgggtgcg atcgcggcag tttttcgggt ggtttgttgc catttttacc
     3421 tgtctgctgc cgtgatcgcg ccgaacgcgt ttcagcggtg cgtacaatta agatattacg
     3481 ttaaattgtg agggaatggt ccccatatac tgactttatc atagggatag ggttggtagt
     3541 gggtcgattg ggtttcggca ttgtttccgt taatgccaat tgcatttttg tatttgatgc
     3601 aacagaaggc gtgagattct cctgggcctg gaaaggataa gtgtttagaa ttgatggcaa
     3661 aatatcttcc atccgatttt tgaagtagtt gttgtttggc tgtctcccaa agggtgttgt
     3721 tacaaacttc ttgaatttgt agtttttgca ggactcttat tactgcgtta tcatgttcca
     3781 attctttagg ggagtgtatt attctctctt ttaataagct gtttaccaca gggtttagtt
     3841 gttgtctaaa gcacgcagat aatgccattg ctacacaatt ttgttcccga ggaagcatgt
     3901 ttggcggggt atgccgtgtt tgatgtgata tgtctattct catagaaggt ctctggctta
     3961 tagcatcaac aaatacggct ataaaacaaa attttcatag agtgattcaa gatcttcttg
     4021 agatcctttt ttgcggcgcg taatctgctg cctgcaaac
//
LOCUS       DAJTRB010000206         3609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTRB010000206 DAJTRB010000000
VERSION     DAJTRB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.210"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            31..510
                     /locus_tag="OO330_004225"
     CDS             31..510
                     /locus_tag="OO330_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884842.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
     gene            498..731
                     /locus_tag="OO330_004226"
     CDS             498..731
                     /locus_tag="OO330_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5884843.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            731..2197
                     /locus_tag="OO330_004227"
     CDS             731..2197
                     /locus_tag="OO330_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5884844.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            2088..2831
                     /locus_tag="OO330_004228"
     CDS             2088..2831
                     /locus_tag="OO330_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5884845.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            2835..>3609
                     /locus_tag="OO330_004229"
     CDS             2835..>3609
                     /locus_tag="OO330_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5884846.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSD"
BASE COUNT          922 a          890 c         1030 g          767 t
ORIGIN      
        1 atcatcaaac tgtcttggat agaagccgca gtggatgcgc acaaaacgtt aaatttcgaa
       61 cccagtggaa gaaagcgtat tggctttgac gtggctgaca gtggtacaga taagtgcgct
      121 aacgtttacc gtcacggatc cgttgttttc tgggccgacg aatggaaggc caaagaagat
      181 gaattactga agagctgcca gcgtacttat caggcggcgc tggagcgtga agcagatatt
      241 gtttacgact ctatcggtgt tggtgcgtct gccggtgcta aattctctga aattaacgct
      301 gaccggaaga gcgagaacgc atacgcgcga cgtgtgaatt accagaggtt taacgccggt
      361 gctggtgtgc atgagccaga tgacgagtac aacggcatcc ccaacaaaga ctttttcgca
      421 aatcttaagg ctcaggcatg gtggctgacc gtttcagaaa tacgtttaac gccattaaca
      481 acggagaaca gtatcctgtg gatgagctga tcagcataga ttctcgttgt ccgttgcttg
      541 aaaagctgaa actggaactg acaacacctc atcgtgattt cgaccgtaac ggacgtgtga
      601 tggtcgaaag taaaaaagac ctcgcaaaac gcgagatacc gtcaccaaac gttgctgatg
      661 cattcattat ggccttcgcg ccaatcgata catcgctgga tatctgggaa cagctgggga
      721 gacaggcctg atggcacgaa acaaacaagc cctgcggcga actgtgcagg ccacagctga
      781 tggttatgag aattttattg cccgagtagg gatgcagaca cctaaccagc actcagcatc
      841 cacctaccgg gctaatttca ccagtcgtaa ccgcatgctg gtggaatggt cctatcgttc
      901 atcctggatc atcggcgaag cagtcgatgc tatcccggat gatatgaccc gcaaaggcat
      961 tcgcatcact tcggaaattg atgcaaaaga tcgtggcatt ctcgaatcac aactggatga
     1021 gttgcaaatc tgggatgcgc tgaatgacgt gctgaaatgg tcgcgcctct acggcggcgc
     1081 ggtgggtttc atcatgattg aggggcaggc accaatgacc ccgctgcgac ccgaaaccat
     1141 cggtaagggc aagtttaagg ggattctccc gctcgaccgc tggatggtcg acccggcact
     1201 gacccgccgc attaaagata tggggccgga cctgggtaaa cctgagtttt acgatgtggt
     1261 gaccacagca acgggaattc ctgcctggcg cattcatcac agtcgcctga ttcgctttga
     1321 tggcgtcacg ttgccatttc agcagaagat gaccgagaac gaatggggaa tgtcggttgt
     1381 agagcgtatc tgggatcgtc ttaccgcgtt cgacagcgct actgtcggcg cggcgcagct
     1441 ggtctacaag gcgcatttgc gtacctacag cgtggagaag ctacgcgagc ttatcgcact
     1501 tggtggtcct gcgtatgaag cgttgctgaa gaatatcgac ctgattcgac agttccagag
     1561 taatgaaggc atgacgctca tggactcgcg ggataagttt gaaacccatc agtacagctt
     1621 cagtggtctg gatgacatcc tttcgcagtt tgcagaacag attagtggcg ctgttggtat
     1681 cccactggtg cggttgttcg gacagtcccc gaaaggattt tctaccggtg atgcagacct
     1741 tgccaactat tacgatcgca tcagttcgtt gcaggagagg cgtttacgtc ttccggtgcg
     1801 gcggatactg gacatcatgc atcgttcgga acttggcaag ccgctgccgg acgatttcac
     1861 gtttgagttt aacccgctct ggcaaatgtc tgatgtcgat cgctcaacgg tggcgttaaa
     1921 caccaccaac gcaatcagta cggcgctggg tgatggtctg atgacactga aagccgctat
     1981 gactgatttg cgagaaaatt ctgacgtaac cggcataggg gcatccatta ccaacgagga
     2041 catcgagaat gccgaagatg aagcgccgcc cggcatcggc gaacctgatg acgaaccgca
     2101 ggagccgtca ggcggaaatc cggtatcgaa ccagcctacg caggatagcg agggcggtcg
     2161 gagacatcgt aaatggtcgc tacgatggtt caaatgacag tatcacggaa attattgcgg
     2221 cgctggagcg ttacagtgaa atcatcaccc cctgggcgac aaaggtcgcg gaaaacttta
     2281 ctgcggacct aacccggcag aacgagaaag tttggcggca acacagcaag aacatcagtc
     2341 gcgagctccg caatcttgtg gaaagcgctc ctgtgggcca ggtgatgcaa tccatcatcg
     2401 ccgaacaggt caagtacatc aaatctctgc ctctcgaggc cgcagacagg gtgtacgaca
     2461 tccagaacaa agcgatagag gctgttgtca ccggtggtcg ggcggagcag tttgctaaag
     2521 agattgcatc taccggcgat gttgctaagt ctagggccga tctgattgcc cgaacggaac
     2581 tgggaagagc aacgggcgcg ctggatatgg cccgagcgat agctattggc tcggatggtt
     2641 atatttggcg tacagccgat gatggcgatg tccgagattc ccacgatcac atgaaaggta
     2701 aattcgtccg ctgggattca cctccaactt tggacggcat gaccggccac gcgggcgagc
     2761 tgccaaactg ccgctgctat aaagagatcg tgtttgttcg cgttccattc gcaatgaaaa
     2821 gggcagcata acccatgaaa tacttttttg agaccaggct cggggaaacc cgataccgcc
     2881 tggctgacgg ctcgttgctg tgcaaagacg tgccgatagg acgaacaggt aagcagctct
     2941 atggtgctga tgacctgcca aaactgaaac ccgataagtt cgttgaaata gtcgtcacgc
     3001 gttctcctga gcaggtattc catccggcca cgcttgcctc attcgaaggg atgagcatca
     3061 cgatcctgca tcctgaagat gaaaacggga atgtgcggct ggtaaatccc gagaactgga
     3121 aagtgcttgc tgtcgggcac ctccagaatg tccggcgcgg gacgggtgag cagtctgatt
     3181 tgatgctggc tgaccttatc gtcaaagacg aaagcgccat tcagcttatc gaagatggcc
     3241 tgcgcgaagt gtcgtgcggc tatgacgcgg agtacgagca gaccgagcca ggtaaagccg
     3301 agcaggtcga tattaccgga aaccatgtgg ctcttgtccc taaaggcaga gccggaaatc
     3361 gttgtgcaat tggagacaga gacacaatgg caaatcaaaa gaaaaactgg tggaaccgca
     3421 tgcgtgcggc catcaagaca ggagatgccg acaccatgaa cgaactggtg gagtcggctc
     3481 ccgcatcggt tacaggagat gagggggatt tgccgcaggg cgttaatctc aacatcaacc
     3541 tgtccccgca gcaaccacta ccggacaaag caccagagat gggcggagat ccaaccggcg
     3601 acagtgatg
//
LOCUS       DAJTRB010000207         3588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTRB010000207 DAJTRB010000000
VERSION     DAJTRB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.211"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            30..491
                     /locus_tag="OO330_004230"
     CDS             30..491
                     /locus_tag="OO330_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5884847.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(532..1002)
                     /locus_tag="OO330_004231"
     CDS             complement(532..1002)
                     /locus_tag="OO330_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5884848.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1049..1768)
                     /gene="yehT"
                     /locus_tag="OO330_004232"
     CDS             complement(1049..1768)
                     /gene="yehT"
                     /locus_tag="OO330_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5884849.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1765..3450)
                     /gene="btsS"
                     /locus_tag="OO330_004233"
     CDS             complement(1765..3450)
                     /gene="btsS"
                     /locus_tag="OO330_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5884850.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          951 a          927 c          816 g          894 t
ORIGIN      
        1 tttcttgatt aaataaaatg gagttttaca tgaaggcttt caataagctg ttttccctcg
       61 ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg tgacaaagaa gaatcgaaga
      121 aattcagcgc caatctgaac ggcactgaaa ttgccattac ctatgtctac aaaggtgaca
      181 aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc ctccattggt gcaaccacca
      241 aagaagatgc tgccaagaca cttgagccgt taagcgccaa atacaaaaac atcgcgggtg
      301 ttgaagaaaa atcaacctat accgatacct acgcgcagga aaacgtgact atcgatatgg
      361 aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat caacgtttct gctgaagatg
      421 ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat gaaagccgct ggttttaaag
      481 aagtgaaata atctgtcggc ggccatgttt cgcatggccg ccatacccgt cttagctttt
      541 cttcacatgc tggcgcgctg ccagtccacg cagaaaataa cgtaaaaatt gatcgccgca
      601 ttcgcggaag tttttatgat ccggtgcgcg catcatcgct gtaatttccg gcatcgaaac
      661 gcggaactgc tgttcggtga ggatagccag aatgtcatcg gttttcagcg aaaacgcgat
      721 gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt tccggctcca gtgccggagc
      781 agactcatcc ttgccgcgtt tttcataaat caggccattg aggaatgacg acaaaacaat
      841 gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt agccagacgg cgatctgttc
      901 cgcggtggct tcgacattat ccagcgccag aatacgcacc aggtcattat tattggcttt
      961 caaaatgtag cgcacgctgc gcagaatatc gttacttatc atgaggcctt caggtgttga
     1021 tcaggcaaaa agccatttta gcagtctttt acaggccaat cgcctctttt aagcttttca
     1081 gataacggcg gctgaccggc acggttaagc cattacgcaa aatcaactcg gcctggccgt
     1141 tatcttccag acgaatctcc tgtaaatgcg cgaggttaac cagatactga cgatggcagc
     1201 gcagtagtgg tgtacgactt tccaaggtac gtaatgtcaa ttcggtaaag ccctctttcc
     1261 cttcgtggct ggtaacgtag acaccgctca tccgactgct gacaaatgcc acatctttca
     1321 tttgcagcaa ataaatccga ctatgccccg tacaagggat aaatttcagc gcctgttgat
     1381 tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg acgcaatcgc gccagcgttt
     1441 tctccagtcg cgcttcatca attggcttca gcagataatc aaaggcatgt tcttcaaagg
     1501 ctttaatagc gtattcgtca aacgcagtga gaaaaacaat atacgggcga tgttccgggt
     1561 caagcatccc caccatttcc agaccactga tgcgcggcat ctggatatcg agaaacagca
     1621 catccgggcg cagtttatgc accgcgccga tcccttccac ggcgtttgaa cactctccaa
     1681 cgatttcaat atcgctctgc tcctgcaaaa atatacgcag gttctcccgt gctaacggtt
     1741 catcatcgac aattaagact ttaatcatgc ctcgtccctc catggtagtc gtaacgttat
     1801 tcgggtgtaa ctatcaggct cacaggcgac gcttattcca tagtcatcgc caaaccgttc
     1861 acgtaaacgc ttatccacca gattcatccc cagcccactg gcattggtta ccggttgata
     1921 caaaccggca ttgtcttcga tctccagcat caaatgttgc ccctcacgtc gggcgctgat
     1981 tgccactcgc cctgtatcca gcagttgtga tgtcccatgt ttaatggcgt tttccactat
     2041 cggttgcagg gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa tagcaatgtt
     2101 gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc agataagcgt tcacatgttc
     2161 aatttcgtcg gcgagagtaa caaactccga aggccgcttt aagtttttgc ggaaaaaagt
     2221 ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg cggcggatca ccgctttaat
     2281 ggtgttaagc gcattaaaca aaaaatgagg attcacctgg gcgtgaagca gtttgatttc
     2341 tgactgggtg agcatcgctt tttgccgctc atattgcccg gcaagaatct gcgccgaaag
     2401 cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa ctgaataacc ggtttttggc
     2461 ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg ccacgcaacg gaattaccag
     2521 cgtcgacccc agtttgcatt gcggatgcaa agagcaacga taaggtactt cgttgccatc
     2581 agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg taagtcgaag aaatcggttt
     2641 gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc agcaatttct ctcgatcggt
     2701 aatcgcgact gcaccaatat ccagctcctg atacagtacc tgagccactt tcatgctgtt
     2761 cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag gctgccactt tcagcgcagt
     2821 ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt ttatcgagca atatacgcat
     2881 aaacagcgcc gcgccgacgg tattggtgac catcattggc gcagcaatat tactcaccag
     2941 acgcaccgca tcttcataag gtcgggcgat cgcaaggatg atcagcattt gcaccatttc
     3001 agcgacgaac gtgacggcac cggcggtaat ggggttaaag actttatcag tgcgcccgcg
     3061 acggatcagg atgctgtgta ccaggccgcc gagcaatcct tcaacgatgg ttgagatcat
     3121 gcaacttagc gcggtcatgc cccccatcga atatcgatgt aagccgccgg tcagaccaac
     3181 cagcccaccg acgaccggac cgccgagtaa gccgcccatt accgcgccta tcgcacgggt
     3241 attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc agaagatgga
     3301 aaagacgatg tagcagagaa atttatgcgg cagacgaacc gtgacctgca ttaacggtat
     3361 gaataatggc gttttactca ttaaccacgc aatgactaaa aaaacgcaca tctgctgaag
     3421 cagcagcaac accagattaa aatcgtacat acccgcaaac cacacttccc tttaaaacgc
     3481 gtaacataca ttgcctgcgt ttaactttct ttgaactctt gcagaaaaat gagaattcgt
     3541 gagtacgatc actcaaaatc gcctggcaaa aataaaatca ccctatag
//
LOCUS       DAJTRB010000208         3479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTRB010000208 DAJTRB010000000
VERSION     DAJTRB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.212"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="OO330_004234"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="OO330_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5884851.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="OO330_004235"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="OO330_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5884852.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..>3479)
                     /gene="ynaI"
                     /locus_tag="OO330_004236"
     CDS             complement(2466..>3479)
                     /gene="ynaI"
                     /locus_tag="OO330_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5884853.1"
                     /translation="TNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHAVQFLIY
                     TIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKK
                     GRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSN
                     FFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSI
                     SVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFA
                     DSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPE
                     QGR"
BASE COUNT          989 a          754 c          805 g          931 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgt
//
LOCUS       DAJTRB010000209         3466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTRB010000209 DAJTRB010000000
VERSION     DAJTRB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.213"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..180)
                     /locus_tag="OO330_004237"
     CDS             complement(<1..180)
                     /locus_tag="OO330_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5884854.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="OO330_004238"
     CDS             complement(321..875)
                     /locus_tag="OO330_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5884855.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="OO330_004239"
     CDS             complement(872..1162)
                     /locus_tag="OO330_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5884856.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="OO330_004240"
     CDS             complement(1162..1761)
                     /locus_tag="OO330_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5884857.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            complement(1800..2087)
                     /locus_tag="OO330_004241"
     CDS             complement(1800..2087)
                     /locus_tag="OO330_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884858.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(2337..2492)
                     /gene="hokD"
                     /locus_tag="OO330_004242"
     CDS             complement(2337..2492)
                     /gene="hokD"
                     /locus_tag="OO330_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5884859.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(2720..3385)
                     /locus_tag="OO330_004243"
     CDS             complement(2720..3385)
                     /locus_tag="OO330_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5884860.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          870 a          918 c          780 g          898 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcagaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgtgcca tcggctttct ccggtggcac ggtgttacac agcaggagtt
     1801 caatcctgct caagattgta gatgagttta ttcttcagca aaagcagaaa accagcctta
     1861 aaaccaatct ttttcaaaac ccgtaatgat gtgacaaatt cgtcctcacg caaaataaaa
     1921 ccgtccgtca gaagtccatt acaaaaataa aataacacag caccgctctt cctttgttga
     1981 gattgcaaac atctaatgcg gcaatggctg acaatcgctc cattctcaac gcgcacagca
     2041 tagaggccat tttcactaaa aatttcacgc aattcttcga ttttcatctt cagaatcctt
     2101 ccagataaat agctctcccc tgttcggggt ccatccctct tctccctgcg cgctacttaa
     2161 gtgagtcgat tctatctgcg aaggtgcgcg aatcaaattc accggaaata aacaacaaaa
     2221 aacccgccga aacgctgaga gatcacagcg atgggcgggt taagtgcggg tgcgttgagg
     2281 atgcctgaca catcagaggt gggcggggat gggatcagct ccccacccgg tcactcttac
     2341 ttcctggatt cgtagtctac gaagacagcg acctccgtct ggccggttcg gattcgtacc
     2401 tcacagaggt ctttcctcgt taccagtgcc gtcactatga cggttaaaca gatgacgatc
     2461 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcatg
     2521 taagaggcta acctacatgt gcaaagcatg aaattggcct cagattaatg ttaagcgtct
     2581 tgccggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
     2641 aagcagatag cctcaagcac ccgcagtcat tctacttacc tcgccaatat gaaatcaatc
     2701 agaaaggtgc cccataaaat cactccttct cttctttacc gtagtggagt tgaccaattt
     2761 tgataagagg gcgtccctga gatttgcggt gtagattggt atcgcgcaga gaatacacac
     2821 agccacaata ttcctgctga tagaattttt cgcgcttgct gatttcaatc atacgggacg
     2881 agccgccctg cttgcgccag ttataatccc agtacaccat gcccggataa tgcgcgacgg
     2941 ctcgccgccc acagtcgtta acctgctgca tatttttcca gcgtgaaatg cccagtgaac
     3001 tgctgatcac actgaaacca ttttcagcgg cgtacagcgc tgtccgctca aaacgcatgt
     3061 caaaacacat ggtgcaacgg atccctcgtt cgggctccca ttccattcct ttggctcgtt
     3121 caaaccagtt gtctgtgtcg taatcagcat caataaacgg cacgccgtgt tgttcagcaa
     3181 agcgaatatt ctcatcctta cgaattaaat actctttctg aggatgaatg ttcgggttgt
     3241 agaaaaagat ggtgtagtcg attcccgagg cctgaagcgc ctccatcacc tcaccggaac
     3301 atggagcaca gcaagagtgc agtagtagtt tgtttgcccc gtttgggagc tccaatttgg
     3361 gccgtttgaa atcagcaact gtcataaata tgtttattga ggtcataaaa atatcaaaga
     3421 gtgtagcatt agaacggggc tatcggaaac agatgtgtga ctgctc
//
LOCUS       DAJTRB010000210         3398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTRB010000210 DAJTRB010000000
VERSION     DAJTRB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.214"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(106..1815)
                     /locus_tag="OO330_004244"
     CDS             complement(106..1815)
                     /locus_tag="OO330_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5884861.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OO330_004245"
     CDS             complement(2247..2966)
                     /locus_tag="OO330_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5884862.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3091..3398)
                     /locus_tag="OO330_004246"
                     /pseudo
     CDS             complement(3091..3398)
                     /locus_tag="OO330_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1001 a          682 c          581 g         1134 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgatcatg gtgggaaggc ttagaacgcc atattcagtt tcccatagtg
     3121 gaacatcact cccagcttaa agcgctcccg gttgcggtag cccttggctt ttattctcag
     3181 cagcctgatc ttgctgttca gtgcttccgc atttccgttt gatacgccat ggcgcatggc
     3241 gttcaggata ccgtacaacc ttttccctat cgttttagcc acattcttca tcatggggac
     3301 gtcactgttg gccgcagcca tctctgccag tcgttgcgtc tttcctcgct ccatggtctg
     3361 ctccagatat cctttgccag ttctttcagc gcccagca
//
LOCUS       DAJTRB010000211         3325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTRB010000211 DAJTRB010000000
VERSION     DAJTRB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.215"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            453..1280
                     /locus_tag="OO330_004247"
     CDS             453..1280
                     /locus_tag="OO330_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5884863.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1324..2073
                     /locus_tag="OO330_004248"
     CDS             1324..2073
                     /locus_tag="OO330_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5884864.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2070..2639
                     /locus_tag="OO330_004249"
     CDS             2070..2639
                     /locus_tag="OO330_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884865.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2763..3005
                     /locus_tag="OO330_004250"
     CDS             2763..3005
                     /locus_tag="OO330_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5884866.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLVEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3009..3155
                     /locus_tag="OO330_004251"
     CDS             3009..3155
                     /locus_tag="OO330_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884867.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEDWCHCRG
                     LSVV"
     gene            3164..>3325
                     /gene="xisR"
                     /locus_tag="OO330_004252"
     CDS             3164..>3325
                     /gene="xisR"
                     /locus_tag="OO330_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5884868.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          797 c          855 g          745 t
ORIGIN      
        1 cgccatcctg acaagcgttt tatcttcaac gggaatgaga tatggaaacg tggagaatca
       61 accacagagt atacccgcgc acaccagcga acaatcagac gactgcgcag actcatcgcc
      121 aggaaacagt atacagaaaa atggttcaga aaatatgaca catggagcgc cggatattac
      181 gaactgatgg caacaaaacc attctgacgt aaacgaaatt aaccatgacg caattaaata
      241 aggcaagccg aatacatcag gaggaccatg aacatttatt tcagaatagt tatatcactg
      301 gcaattatcg catgtattta cggattaatc gttccggccc tcatatcaat gaaggatacg
      361 gtagcagtga tttctggctt tgctctggcg tgtctgaccc cgccctgcat ttatgccatt
      421 tataagggtc tttctttttc taaggataaa agatgaaaaa agttattcct gctttagccc
      481 ttgttctgct gaccaccgga cttgtcggtt gcgatcgcgt tgagcctggt aatgttggca
      541 tcaaggtaaa caaactgggc gacgataaag gcgtcggaga agtggtcggt gttgggcgct
      601 actggacggg atggaacact gaggtttaca tcttcccgac cttcaaacaa atgaagacct
      661 acgatgagcc attcagcttc cagatgagtg acggtacaac catcggctat cacatcggcg
      721 tggcctacaa agttgatcca tccaaagtta ccacagtctt tcagacctac cgcaaaggcg
      781 tggacgacat taccgacacc gacctgcgcc agaagatagc cgacgcactc aaccgactgg
      841 ccagcaaaat gaccaccgac aaatttatcg acggcggcaa gtctgagctg ctggatgcag
      901 ctcttaaaga cattcaggaa gaaatgacgc ccatcggtat tcaggtaatg agcctctcat
      961 atgtgggtaa gccggagtac ccgcctactg ttatcgacag cattaatgcc aaagtcacgg
     1021 cgaaccagaa aaccctgcaa tgcgagcagg aagtaaaaca gcgcgaagcg gaagccaaca
     1081 tgttgcgcgc ggaagctgcc ggacaggcag atgcgattcg cacaaaagcc caggccgaag
     1141 ccgatgctat tcgtttacgc ggtgaagctc tgcgccagaa cccaaacgtt atggagctgg
     1201 aagccatcaa caagtggaac ggtacactgc cgcaatacat gaccagcggt gccaacacac
     1261 catttattca ggttaagtaa tacatatgcc cggtattaca cgccgggctg tctggagata
     1321 aaaatgaata ctgtaaccat caacaacaaa cagtttccgg taatcgaata tcgcggtcag
     1381 cgtgttgtga cgctggcgat gattgatgaa gtccaccagc gaccggaagg aaccgcgcgt
     1441 gcagcattca accgaaaccg tgagcatttc atcagcggtg tggattatgc cgaattaggt
     1501 gcggacgtaa tacgtacgga cctcccggaa ggaacattct ctaaatttgc accatcgggg
     1561 attgtacttt ttgaatcagg ttacctgatg ttgacgaagc cattcaatga cgcccttgcc
     1621 tggcaggttc agcgcgaact ggttaacagc tacttccgca ctcgcacacc gctaacggaa
     1681 atcgagatga tcgctgcgat agccgccgac gctgttcgtc aacagaagcg cctgaatcat
     1741 gtggaagaac agctcgaaac agtcacagaa gcggtggaaa ccatcaaacg cggaaatatg
     1801 cgcgccggat atgtcggtta ccgccaggtg gtagccaaaa gtggaatgag tgacaccaag
     1861 tgtcggaatc tggtgaacgc atatcaaatt cctacagaca cccacgaatt catgactccg
     1921 gacggtctgt tgtctcgcag ggctatcgtc gagcttgagc catttatggc ggcgttccac
     1981 caaatgatgt cagaggctga cccacgcggc acacgctggt atcacccgaa gatggggctt
     2041 ttccaggtga tcggatggga ggataaagca tgatcatcca gtcaaaactt attcgcgccg
     2101 ctctggtgtg cgctgctaaa aacgacgttc gttattacct gaacggtctt cacatcacgc
     2161 cgaaacatat tgaggcaacc aatggttccg tggcactgcg catggctcac ggcatccgga
     2221 cgaagaaaaa catcattgtc cagttcgaag ggggcgtccc ggccaaagcc gaaacgacag
     2281 agctgatttt tagtaaagag ccgatcgctg ttcatcgcga ccagtttcag cgacgactgt
     2341 ccattaccgg cattaaactg gtggacggtt gttttccgga tttggatcgc atcattccga
     2401 aaaagtttga ccgctgtact cacccggtgc tccaggcggg ttacctgagt tatccagaga
     2461 agatgtttgg tcgtgagcgt aaatttattc ccgtccagtt acgtccctcc ggtgacgggc
     2521 aagcggtcag aattcagttt gattccatca tcaactcaat gtatggcaat cctgaatttg
     2581 ttgtgatgcc ttgtcgtgat catggcgatt tcaacgtggc tcaggagcat ccggaatgaa
     2641 aatcgaatac caggacgccg caggaggtga atcaatgagc tggcctgatg caatcgtaac
     2701 tctggggatg gtattcgcag tagcgtttgt tgtgtactcg atttgtcgat ggggataacc
     2761 acatgttcgc tttgattcaa cgcgggcaga tatacacaga cagagccgga tacccagtag
     2821 aaattactcg cagtactgag cactcagtgt tctttcggag gatggacgga cgtaccgggc
     2881 gagtacgcat cggtgaattc agtagcctgg tcgagcatat tgaccacctg gaatatcaca
     2941 agattctggc tgaaacagaa caagaaaagc acctgaagaa attacgcgca atgcaaagga
     3001 agtaaaaaat gaataaagcg tttgagctat gggtccacca gcgttacggc aaccactacg
     3061 acctgacgcg agatgttgac ggtttctact gtcgtgaaat tgtgaaacga atgtttgagg
     3121 actggtgcca ctgccgtggg ctgagtgttg tgtgaggtaa tgcatgggca atgtgattca
     3181 actggctccc aatgaatggg tttgtgaaag cgttctaatc gcaattaccg ggctcaaacc
     3241 aggcacaatt cttcggaccc ggaaagaatg ctggatggtt ggaagagagt atattcacgt
     3301 atcaccagac ggtaatccaa agcct
//
LOCUS       DAJTRB010000212         3291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTRB010000212 DAJTRB010000000
VERSION     DAJTRB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.216"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..440
                     /locus_tag="OO330_004253"
                     /pseudo
     CDS             1..440
                     /locus_tag="OO330_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1024..1740
                     /locus_tag="OO330_004254"
     CDS             1024..1740
                     /locus_tag="OO330_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884869.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2292..2696
                     /locus_tag="OO330_004255"
     CDS             2292..2696
                     /locus_tag="OO330_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884870.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2759..3291
                     /locus_tag="OO330_004256"
                     /pseudo
     CDS             2759..3291
                     /locus_tag="OO330_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          925 a          676 c          757 g          933 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagacccaga tatgaacagg cacattcaga
       61 gttattttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggccccccgg tttaatctgg
      301 tgataaaggc gttttacatg cgcctacttg cagccggaaa cgccaaaaaa gtggcactgg
      361 ttgcctgtat gcgtaagttg ctgaccatta tgaacgcaat gctcagaaaa aacgaagaat
      421 ggaatgaatc gtatttatga aaactggatt gtgggccata aatattccaa aaagatagcc
      481 cgctccgttt attaccggaa cgggcgagag catgacattg tctgttatta taatgagact
      541 gctgattcta cttattttta atttcaagaa aagcgttttc tacaaagaat tatgagattt
      601 aatgttttgt ttagacaata ttatcattct gttcaataat gaaagtcttc atcaacaaca
      661 agtaaaaaca acgtccctct catcgaaccg gacttgcaga tttcccgcat ccggctctct
      721 tgcaacacat cagaccttca cgttcgggcg gttatgggga aggtccagca agctgtatga
      781 gccctaactg tttgaatacc agtagattac cagaaacttc tatcgattga agacattacc
      841 aactagcgtt actaaatgtt gaccaagttt tgttcacttg tctggttttg atagtagcat
      901 tctgcagagt attttgatat gataaatatg cattaacaat gatattattg ttaatttcct
      961 agcatgaaaa ttaaaagatc aacttttatc tcgaatattt tttacattat cagttggttt
     1021 cttatgaatg acaattcatt gcttagaaat tcttcattat tcatcgccta tatgggatgt
     1081 gtagggtggg tcagtgctta ttcttatggc tggggaacct ctttttacta tggatttcca
     1141 tggtgggttg ttggtgcagg tttggatgat gttgccagaa gtttactcta tgctatcata
     1201 gtaatgggta tactttttac cggttggggg attggtattt tattttttct tcttataaag
     1261 aaaagaagta aaatacaaga cttgagtttt tttagactat tttttgctat taccctactg
     1321 tttttccctg taatattcga acttttaatt ctcaaacaat atttcatact tccattatca
     1381 ctttcattca ttattagttc attggttatt tcaataataa ttcgtatata tggacggatc
     1441 ttttctgtat catgtttttc agatatccct tttgttagag aacaccgcat taagttaatt
     1501 atggctggtt ttttggttta tttttggttt ttttcctttc ttgttggctg gtataaacct
     1561 caattaaaaa aagaatatca gatgctttgt tataataata gttggtatta tgttcttgct
     1621 cgttatgatt cacggctcgt tttatctagt tcatttaaag atgacagtaa cagattctta
     1681 atatttaata cagaacagtc gggattttat gaaatcaatg atgtttatgt cagaaagtaa
     1741 taatcctctc ccacaaagtg aattaaatga atatataatg cgttcgaagt atatgtattg
     1801 agctgtcata ctgagaaaca aaccttaggg cagcacaatt aaagccagaa acaactatgc
     1861 aagaaagtta ctaaataagt gctcggaaca gtaaaacact caggggggac tcagtccgga
     1921 ttatgtaatc agataaattg ccaaacatca caaatcacca gccggactga gtgatgccga
     1981 gcattgcacc aataccccgt gatgaacgat gcctgatgca gaaagctccc tataaaacaa
     2041 agaacaaaaa ctacgaccgg cgattcaccg tcatactgat gctgcatcgt ggtgacactg
     2101 tctgtcactt tcatgttgac tgaacaactc tgctgcgaag aaggtgtacc gccggcggga
     2161 tttgattgtc cggcgtctag cttccacttc cctgatggaa ctgaaagttg ttatggccgg
     2221 tcagcttgcc ctgtccgcag gacatgaggt gctgtgacgg acagcaccag taacggaaag
     2281 tgacaatgaa aatgaatact gcgctgatag ttgcactaat gtgtatgtgg tatgcggtac
     2341 ccgctgctgc aaaagagact ttactggcta tgccacgtaa ttcaacagaa cattgctatg
     2401 cggagattaa tgtgcacgga ccatatgggg tgtatttccg agtggtcccg catccccccg
     2461 ggggaaagag ctgggtcgaa tgtaacagtg actactatta ctctgataaa ccaccgggag
     2521 tacagatact cggaacccgt gcagggtgtc gggtatacgg aatctgcggg accacctcga
     2581 cactgcatgt ggctggaaga ggggttgtgt gtattaaaaa tatatgcagt ccacgtggga
     2641 tgataattca caggatacgg aaacgtcctg tagtggctgt aagcgatgag atgtgatctt
     2701 acccagcaat agtagacacg cggctaagtg agtaaactct cagtcagagg tgactcacat
     2761 gacaaaaaca gtatcaacca gcaaaaaaac acgtaaacag cattcgcctg aattttgcag
     2821 tgaagccctg aagcttgctg aacgcatcgg tgttgctgcc gcagcccgtg aactcagcct
     2881 gtatgaatct cagctctatg cgtggcgcag caaactgcag caacagatga cctcgtctga
     2941 gcgtgaaagt gaactgcccg cctgaagcgg caactggcgg agcaggcaga ggaactggct
     3001 atcctccaaa aggccgcgac atactttgcg cagcggctgg tatacgtggt gtcagcgtcg
     3061 gacaggaata agccctcgtc agcagttccg tcagcactgc gacagcgttg tcctcgcggc
     3121 ggcctttacc cggtcaaaac agcgttacgg tgcaccacgc ctgacggatg aactgcgtgc
     3181 tcagggttac cactttaacg taaaaaccgt ggcggcaagc ctgcgccgtc aggggctgag
     3241 ggcaaaggcc tcccggaagt tcacgtatag gaaattgaaa aaccagacga t
//
LOCUS       DAJTRB010000213         3268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTRB010000213 DAJTRB010000000
VERSION     DAJTRB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.217"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..358)
                     /locus_tag="OO330_004257"
     CDS             complement(<1..358)
                     /locus_tag="OO330_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5884871.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDS"
     gene            604..1365
                     /locus_tag="OO330_004258"
     CDS             604..1365
                     /locus_tag="OO330_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5884872.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(1472..1972)
                     /locus_tag="OO330_004259"
     CDS             complement(1472..1972)
                     /locus_tag="OO330_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884873.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(2123..>3268)
                     /locus_tag="OO330_004260"
     CDS             complement(2123..>3268)
                     /locus_tag="OO330_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5884874.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPRATGVNVNGWPGVSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT          983 a          727 c          584 g          974 t
ORIGIN      
        1 gagaatcatc ataagattgt ttacttattg ttatgttttc actgatgtaa aagcctactt
       61 tgggtatcgt aagaatcaca ttttcttcaa caccaatatt tttaaaactt ttgcgtaaca
      121 gggatatata ttggttcaat gtgttgtttg acagatcaaa tgaaaatctc tccaaaacgc
      181 tatcaatgat tgagttcctg tcaaccgctc caccattact ttccagtaac actgccaaaa
      241 tacaccccgc cgtagctggc aaatttatct catcctgggt atttacattc gagagcgagc
      301 atttgttcgg atcaaaaata atcgcgtcat tgataatgaa aaaacatctc cgctccatgc
      361 aggcaacaac acaaaataag attttgtcat tttagcctat tcctggtgga actgacacaa
      421 gaaactaaaa tacaaatata accctgaaaa agtcatataa aacttatatc cacagaataa
      481 aaaatcaaac atgtcagttt tatcaatata aagtatcgca aggcgaaaag tgatgcaaga
      541 atactcctaa gtaaatgaaa gcgcaccaca tgaaattatc aacacatgta atttcatcat
      601 taggtgctat cgaaaataac tcaggagcta cttaagatga ataatgtctt actcatgaga
      661 cccgatgact cagaatttat gatacctgat gactggtcag ggctaaacgc aaaaggcatt
      721 acatacgaac aagataatag agtgacagag tcttttctgc cttttaaatc tttttattgt
      781 tcaccggtgt gcacgacagt tgaactcatc tcacaaaata attgttttgc tatgggtatc
      841 cgctaccttc tggaaaagaa aagagactta aggctaaatc actttaaagc cttctctcat
      901 aaatgcgaga caaaacctga cgtgttaatt atgaccgggt gcaccgtcgc tgatacttat
      961 agtatattct cacgcattgt tgaagaaaat ataatgaaaa cgatgacacc tatcgtcatc
     1021 attactgggc atcactcaga agatctttgt tctctggcat cgaaactggg aggagttaaa
     1081 ataatatcac cgacagaaaa gattgcaaaa atagaaaacg ctatcaatga tgcttttaac
     1141 agcaagccta aaaaaataag atttttaaca gatatactct ctcctcaaca aattgagacc
     1201 atgaaaatgg ttgcgcaggg tagatccgtt aatgagattt cagccgcaat gaatattagc
     1261 atcaagaccg tgtatgcaca taaagttcgt gctatcaata agctgggaat taagaaaaaa
     1321 gtttatgaag cgctttttta taaagcgcta attcagttca actgatgtaa aaatcaccct
     1381 cgcttaataa ataatatatt ctatgtcttt ttttataagt taaaaaaggc gggatatcaa
     1441 accccgccat gaaattaact aatcatctgc attattctgc gtagatataa gcctgggtac
     1501 ggatggtgta agagtctggc tcaacaacct gttctttcat atgggaaacg acgcgatagg
     1561 caccacctgt tccagtattt ttcagcatca tataacgctt accgccaatc tcttcggagg
     1621 cagctgaagg aggatcctgc tcaagcgttg tcagggaaac tttcagttta tggtccgggt
     1681 tattgtcacc agaaattact gcatccgctg aagtactgct ctccgcataa atatcatccg
     1741 ggaaagttac agcatagtcg tggctctgcc catcagatgc gccaacaata cctgttgcca
     1801 aaggcgtcca tgcagttaac gtaccgctat ataccccttt cactggttga atggtattat
     1861 taacaacgat aggcttggtc aaagtaagat tagctgacgc ttcagtaccc tggcttacta
     1921 aagttcccgc ctgagaagca aaagctgtcc ccgccagcat gattgctaac aatgttttct
     1981 tcattatatt ttctccatga ttttcattta cactgcaaaa tgcagtttct ttctttgcat
     2041 ttacgtgcta tcgcgaaaat gcttccacca aataatggaa acatcttcac aaaaataaat
     2101 tatgagtttc ttaatttaga aactataaac atgtcgccgc tatctggtat aaccctgatg
     2161 cacttttctt cgcaggaagt tggtattttg cgatacagga ttgatcttta gaataacccc
     2221 atttcacatt aaactcgcct tccgcactca agccagaaac ataagcaata ccatcttcac
     2281 ctacaatgcc ggccatattt tcagtgtctt tgctataact aatcacagcg ccgaagggga
     2341 cgggtttacc ctctttctgt tttaaggtta ctaatccttt atccccgctc ttcactgcca
     2401 atgtcgcagg cacaacggct ccagctgtcg gtacaaccaa ttgactagtt tgtggtaagt
     2461 cgtaattctt acctatcgtt ttttcatcca gaataacatc gtctcttcgg tatggctgca
     2521 ctggcagtat tgcataccca cgaaaatcag tactcactcc cggccagcca ttaacgttaa
     2581 cccccgttgc tctgggtgcc tctataaggg ctaatgtccc atccacattt tgaccaaatg
     2641 taagtccatc ccttgtaata agtatcccac cgcttaaatt tgcacctagc tgtttcatgt
     2701 tttcgctgga gctgtaatgg ctactaaact gaccatatga tccatttttg ccaacttgca
     2761 caatgttcat cgtgctcttc ggttgtgact gatgagcgtt agttatattt gtcgctacat
     2821 cccaccatac accaccacta ttctgacctg ataatgaaac ctgatagtta ttaccctgct
     2881 ctcccgaacg actcatctgt gaagacgcat aaatttgatt ttcgtcaccc agcgataatg
     2941 gcactgaaat acccacgctt aatttccgat ctactttcca tttatgcgat gtcagcaagt
     3001 cgttttttgc ccagttaatg gagaatgtta tgcctctcca taccgtacta taactcaggc
     3061 tggtagaccg cgtgtttcct gtgccccggg aagaattata cctcgtggta ttgaagttca
     3121 aattcccgat attaccaagg ggctgggata aggatatcgt agagccatcg tgactattct
     3181 tatctctttt tctgttccgt tcatcctgtg cacttactgc ttcagtcagt gtggcataac
     3241 ctgatgcata acggttcgtg gttataga
//
LOCUS       DAJTRB010000214         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTRB010000214 DAJTRB010000000
VERSION     DAJTRB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.218"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO330_004261"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO330_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5884875.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO330_004262"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO330_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          802 a          659 c          773 g          945 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgtt aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggta gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTRB010000215         3161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTRB010000215 DAJTRB010000000
VERSION     DAJTRB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.219"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..756)
                     /gene="ipaH9.8"
                     /locus_tag="OO330_004263"
     CDS             complement(<1..756)
                     /gene="ipaH9.8"
                     /locus_tag="OO330_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5884876.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQQNTLHR"
     gene            complement(1018..1113)
                     /locus_tag="OO330_004264"
                     /pseudo
     CDS             complement(1018..1113)
                     /locus_tag="OO330_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1149..1454)
                     /gene="ccdB"
                     /locus_tag="OO330_004265"
     CDS             complement(1149..1454)
                     /gene="ccdB"
                     /locus_tag="OO330_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5884877.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1456..1674)
                     /gene="ccdA"
                     /locus_tag="OO330_004266"
     CDS             complement(1456..1674)
                     /gene="ccdA"
                     /locus_tag="OO330_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5884878.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2210..3161)
                     /locus_tag="OO330_004267"
                     /pseudo
     CDS             complement(2210..3161)
                     /locus_tag="OO330_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          785 a          763 c          770 g          843 t
ORIGIN      
        1 gcgatggagt gtattctgtt gtccgtcact catggagaag taaatccgtg ggccgtggta
       61 gtccggcgaa gaggttaatc tttgcagggc ttgcagagca tgggatgata atgggttatc
      121 actaatatgt attgaacatt cattcctcag attaagaatg ctttccggga tatgacttat
      181 ctgatttcta tcaaaaaaat actctctgac aacgggatta tttccctcag aaaatgctgg
      241 tagctcagtg aggaaattac gggtcgctct gagattttta agagcctgtg ggagcgatgg
      301 taaggagact atttcgttat agctgatatt catagtcaat aacgaatcgg gtaaagctgg
      361 caggttttcc agctcattgt gttgtacctg aagtgactcc agcgcaggag gtagcacggg
      421 caattctgat aatttattgc tggcggaata taattttttt agcgtaacag gcagttcagg
      481 taggttagtt aattgattat atgatacatt gagcagcgtt atctgagctg gtaagttgtc
      541 aggtagcgag gacagattta aacggtccag tcgaagttca tcggaattat tgataagaca
      601 ttctttaagt cgggagacag cctcatcacg ctcttcaccg gggagcgctt gtttttccca
      661 tttatcccat gctgaaaagt aatcagcata tgtaccggaa atagtgttat aaaaagaatt
      721 ttggggcaat gaaaagttat tatttatcgg taacatgatg ggcatccagt taaagccggt
      781 ggacaaatgg ctttgttaaa agaaataagt aggagttttt atgaagttca ttccatgaaa
      841 tgaacttcag cactttttta ttataataag gataaacaaa attggctgta aaaaaacgat
      901 actgtttcag ttaaccgtga atggcctgtt tgcaccgtat tgttcttgtt tttcggttag
      961 ttaaacatcg tacttcatat ttgaacggta agcggctcgt cagaaccgta ttgatattta
     1021 ctgagaggtc agatcaactt tccaaggcaa cagatcgcgt acccggtttg ccggccagtc
     1081 ctggatatgc tcaatgacgt aacgcagcca ttttgtcaac gccacgattt ttgaccctag
     1141 cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
     1201 tggctgagat cagccacttc ttccccgata acgaagatcg gcacactggc catatcagtg
     1261 gtcatcatgc gccagctttc atccccgaca tgcaccaccg ggtaaagctc acgggagact
     1321 ttatctgaca gcaggcgggc actggccagg gggatcacca tccgtcgtcc gggcgtgtca
     1381 ataatgtcac tctgtacatc cacaaacaga cgataacgac tctctctttt ataggcgtaa
     1441 accttaaact gcatttcacc agtccctgtt ctcatcggca aaagagccgt tcatttcaat
     1501 aaaacgggcg atctcagcca tcccctgctg attttcagcc tgccagcgtt ccgcacgcag
     1561 acggcgggcc tctttttgca tggccgtatt taccaggcct gagatattca cgtttgcgga
     1621 tttaagtaac tgatagctgt cactgtctat agtgacagta atacgctgct tcatgataag
     1681 ccctcaaaaa catacttatg atatgtatat gagtatatat taatggtaca aaccatcaat
     1741 atccataaaa agtacaccat taattcgcta taagttttta atacaatttt ttctgctgcg
     1801 atttttttat actataatta gcatacttat aacaccgtaa gtaaattaca tgaaagcagg
     1861 cacagcgtga tccgattttt ctgcgtataa cgattttccg gaagtgtaat acggttgcgc
     1921 cccgggccgg gcaccctttt ctgttgttgt gtacgggccc acagagaatg gtaccggagg
     1981 cgggctcaga agtactgagt cgggtgatcg cgtcaggccg cgaagtactg acacgggtat
     2041 tggtgactgg tgatgaagta ctgccccggg tatgatttca ggctgattca ggttctgatt
     2101 ctctgtcagt ccgcgtggtt gctggcctgg ctgatttcct gttgtcaggc gttcaacaac
     2161 cgactgcagg ttatagcccg gttcacgaac acctgacggc aggtatcact cacccggtga
     2221 aggtaaatcc ctcaagaggc agcaactctg ccagtcgctc ctccggccat tccggcagac
     2281 gcgtcaggac gtccgtcagc caggcatgtg gctccagacc attacgtttt gctgtttcca
     2341 gaaggctcat tatctgtgcc gcccggtttc cggccatcag tgaaccggcg aacagccacg
     2401 ctttccgtcc cataacaacc gggcgaatgg cacgctcgca gatattgttg tccaggggca
     2461 ccgctccgtc acccaggaag cagctcagtt catcgcgccg gttcagaata tagttaatcg
     2521 ccttatttaa tggcccgtcg ggcggacagc acggctcctg ctcttcaagc caggaaaaca
     2581 ggtcattcac tatcggccgg gagtaccgtt atcgccactg ccgtattttt tccacagggc
     2641 gctcacgaat gagtttctca atgcggtaaa gacccgcgat ttttctcagc tccacgcctg
     2701 cacggggctc cctgctggcc ttatacagat ttgcgaagcc gcgccctgcg tgactccagc
     2761 aacaggctga tgtgatgccg gggtgattat ccgccagaga tttgtacacg ctgtatccgt
     2821 cactcaccag cgtactgcca caccagcact gcagccaggt ctcagggtat ctgagggccc
     2881 gtccggtctg cgagtcaaaa cagacgaccg gcggaccgct tctttcaccg ctgacgtaag
     2941 cacacagcca gccggagcag gatttaccgc ctttccgggt atcaaggagc ctcagggacg
     3001 tttcatctgc atgaataaca tcccgtgtca gaagctcatg gcgtagcagt ttcgccagcg
     3061 gggccagtgc agctccggca acaccgacca tatccgccat tgtactgacg gggagctcca
     3121 cacccattcg ggagaaaatg tgctgctggc gatacagggg c
//
LOCUS       DAJTRB010000216         3064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTRB010000216 DAJTRB010000000
VERSION     DAJTRB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.220"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..197
                     /locus_tag="OO330_004268"
     CDS             <1..197
                     /locus_tag="OO330_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5884879.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="OO330_004269"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="OO330_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5884880.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="OO330_004270"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="OO330_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5884881.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="OO330_004271"
     CDS             1821..2576
                     /locus_tag="OO330_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5884882.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2977..>3064
                     /locus_tag="OO330_004272"
     CDS             2977..>3064
                     /locus_tag="OO330_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5884883.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVF"
BASE COUNT          797 a          682 c          727 g          858 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataaa tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgtgggct tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt
     3061 tttg
//
LOCUS       DAJTRB010000217         3058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTRB010000217 DAJTRB010000000
VERSION     DAJTRB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.221"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..487
                     /locus_tag="OO330_004273"
     CDS             <1..487
                     /locus_tag="OO330_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5884884.1"
                     /translation="LKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVN
                     CPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRIDDAES
                     GNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVTLDY
                     R"
     gene            567..1262
                     /locus_tag="OO330_004274"
     CDS             567..1262
                     /locus_tag="OO330_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5884885.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1291..>3058
                     /locus_tag="OO330_004275"
     CDS             1291..>3058
                     /locus_tag="OO330_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5884886.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          884 a          717 c          680 g          777 t
ORIGIN      
        1 tttaaaatcc gcttgtgaaa tcaatgactc tgataagaaa attgaagttg ctcttggtca
       61 ctataatgcc gaacagtttc gttctgttgg cgatcgtagc ccaaaaattc cattcactat
      121 tcctttagtt aactgcccag tgactgggtg ggagcacgat aacggcaacg ttgaagcgtc
      181 tttccgttta tggctggaaa cgcgggataa tggcaccgtc cctaacttcc ctaatctggc
      241 aaaagtgggc tcttttgctg gcacagcgac aacaggtgtg ggtatccgta ttgacgatgc
      301 ggaaagcgga aaccttatgc cactgaatgc tatggggaat gataacacga tttatcagat
      361 cccagcggac tccaatggta ttgtcaatgt tgacctcatc gcttactacg tatcaaccgt
      421 tgaagcatca gaaatcaccc caggggaagc agacgctgtc gttaacgtaa cgctggatta
      481 ccgttaatac gttacggcgt tatctgacct ttcagataac gcccttttcc ttcctctttc
      541 tcgttgtatc aggttgaaaa ataactatgt caaaacgaac attcgcggtg atattaacct
      601 tgttgtgtag cttctgtatc ggccaggcgc ttgcaggggg tatcgtttta cagcgaacgc
      661 gagtgatcta tgatgccagc cgcaaagagg ctgcgttacc tgtcgcaaac aaaggcgcag
      721 aaacgcctta tttactgcaa tcatgggtag ataatataga tggtaaaagc cgtgccccat
      781 ttattataac cccaccgcta tttcgtcttg aggctggcga tgactcatca ctgcgaatta
      841 ttaaaacagc tgataacctg cctgaaaata aagagtcgct gttctacatt aatgttcgtg
      901 ccattccagc aaagaaaaaa tcagatgatg ttaatgctaa cgagttgacg ctggtattta
      961 aaacgcgggt caaaatgttt tatcgccccg cacacctgaa gggacgggta aacgatgcgt
     1021 ggaaatcact ggaatttaaa cgtagtgacc attcactcaa tatatataac ccaactgaat
     1081 attacgtcgt atttgccgga ctggcagtcg ataaaaccga tctcacaagc aaaattgaat
     1141 atatcgcgcc cggagaacat aaacagttac cacttccagc atctggcgga aagaacgtga
     1201 aatgggctgc gatcaacgat tatggcggca gttccgggac agaaactcgt ccactgcaat
     1261 aaaaaatata aaaacacagg ccatcaggga atgccacaac gacaccacca gggacataaa
     1321 cgcacaccga aacagttggc gctcattatc aaacgttgtt tgccgatggt gctcactggc
     1381 agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc cattatgctg
     1441 gaaactacaa aaagtggtat gcaaacaacc gatctgtcac gtttttcaaa aaaatacgca
     1501 caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt ttcacagaaa
     1561 aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac ggtagaacaa
     1621 ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa agatgacgat
     1681 agcgtgatca actcgcttga acaaatcatt cccggtacag ctgctgaatt tgatttcaat
     1741 catcagcgac ttaatttgag cattccccaa attgcactgt accgtgatgc aagaggttac
     1801 gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta ctcgtttaca
     1861 ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct gaatatgcaa
     1921 aatggtgcta attttggccc ctggcgatta cgtaactatt ctacgtggac acgcaacgat
     1981 caggcgtcaa gctggaacac tatcagtagt tatttacaac gtgatatcaa ggcgttgaag
     2041 tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag ctacgccttt
     2101 actggcgtgc aactcgcttc cgacgataat atgttgccaa acagccagcg cggatttgcc
     2161 ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca aaatggttat
     2221 gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct ctacccctct
     2281 tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacaca acgtcgcttt
     2341 atccagcctt attcttcatt acccatgatg cagcgacctg ggcatctaaa atatagcgcg
     2401 accgctggac gctatcgcgc tgatgcaaac agtgatagca aggaacccga atttgctgaa
     2461 gccacggcaa tatatggttt gaataatact tttacgctgt atggcggcct gctcggttct
     2521 gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact gggcgcgttg
     2581 tcgatggata tcaacagagc tgacacccaa ttcgataacc agcactcttt tcatggctat
     2641 caatggcgta ctcagtacat caaagatatc ccggaaacca acaccaatat cgctgttagc
     2701 tactatcgct ataccaacga tggctatttt agttttgatg aagccaatac ccgcaattgg
     2761 ggctataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac aatatttgat
     2821 ggggtaagtc tgtatgcctc cggttcacag caagactatt ggggcaataa cgagaaaaac
     2881 agtaatatct ctgttggggt ttccggccag caatggggaa ttggttacag cctgaattat
     2941 caatacagcc gctacactga tgaaaataat gaccgtgcac tctctttgaa tctcagtatt
     3001 ccgttagaac gctggtggat ttgcccctat atttccagac atctgttatc acttaacc
//
LOCUS       DAJTRB010000218         3020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTRB010000218 DAJTRB010000000
VERSION     DAJTRB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.222"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            437..2089
                     /locus_tag="OO330_004276"
     CDS             437..2089
                     /locus_tag="OO330_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5884887.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO330_004277"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO330_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO330_004278"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO330_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3009)
                     /locus_tag="OO330_004279"
     CDS             complement(2596..3009)
                     /locus_tag="OO330_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR5884888.1"
                     /translation="MQGASLATLGYDFKVRTLKNFKGELYRKCMPGASNAAMCKITET
                     IKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSRLLKWMQS
                     KYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
BASE COUNT          887 a          633 c          687 g          813 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggtacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgg
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg ctagactggc
     3001 cccctgaatc tccagacaac
//
LOCUS       DAJTRB010000219         2951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTRB010000219 DAJTRB010000000
VERSION     DAJTRB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.225"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="OO330_004280"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="OO330_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5884889.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="OO330_004281"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="OO330_004281"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5884890.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="OO330_004282"
     CDS             2603..>2951
                     /locus_tag="OO330_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5884891.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          702 c          779 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAJTRB010000220         2924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTRB010000220 DAJTRB010000000
VERSION     DAJTRB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.226"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..1508
                     /locus_tag="OO330_004283"
     CDS             <1..1508
                     /locus_tag="OO330_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5884892.1"
                     /translation="AADDGSERLVSTARTTETTYRFRQLALGNYSLTVRAVNARGQQG
                     DPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDPTVQFEFWFSEKRIADIRQVE
                     TSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKSAFVEAVGRASDDAEGYLSFY
                     KGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSNEITQTVNKKLENQSAAIQQI
                     QKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMIN
                     PANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPGGRLTAKNADISG
                     NVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVGKPFPRDSRAPERWPSGTITV
                     RVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLIVKKNGAEIYNRTTLDNTLIY
                     TGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKSTAGISIS"
     gene            1576..2175
                     /locus_tag="OO330_004284"
     CDS             1576..2175
                     /locus_tag="OO330_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5884893.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            2239..>2924
                     /locus_tag="OO330_004285"
     CDS             2239..>2924
                     /locus_tag="OO330_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5884894.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNL"
BASE COUNT          728 a          680 c          884 g          632 t
ORIGIN      
        1 tggcagcgga cgacggcagt gagcggctgg tcagcacagc caggacgacg gaaaccacat
       61 accgcttcag gcagctggcg ctggggaatt acagtctgac agtccgggcg gtaaatgccc
      121 gggggcagca gggcgatccg gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg
      181 tcactattga actgataccg gggtattttc agataacggt ggtcccgaaa ctggctgtat
      241 atgacccgac ggtgcagttt gagttctggt tttcggaaaa gcggattgcg gatatcaggc
      301 aggttgaaac cagcgcccgc tatcttggca cggcgctgta ctggatagcc gccagtatca
      361 atatcaggcc gggccatgat tattattttt acgttcgcag tgtgaacacc gttggcaaat
      421 cggcatttgt ggaggccgtc ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt
      481 ataaagggtt gatcaataaa acgcatctcg gcaaggagtt gtggacgcag attgataacg
      541 gtcagcttgc gccggacctg actgaaatca ggacgtccat tacgaatgtc agcaatgaaa
      601 tcacgcaaac cgtcaataaa aaactggaaa atcagagtgc tgcaatccag cagatacaga
      661 aagttcaggt tgatacaaat aataacctga acagcatgtg ggctgtgaag ctgcaacaga
      721 tgaaggacgg acgcctttat attgcgggta tcggtgccgg tattgagaat acgccagcag
      781 gaatgcagag tcaggtgctg ctggcggcag acaggattgc gatgattaat cctgcgaatg
      841 gcaacacaaa gccgatgttt gttggtcagg gcgatcagat attcatgaac gacgtgttcc
      901 tgaaacgcct gacggctccg accattacca gcggcggtaa tcctccggca ttttccctga
      961 cacctggcgg acggctgacg gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact
     1021 ccgggacgct caacaacgtc acgattaata agaactgtcg ggttctggga aaattgtccg
     1081 cgaaccagat tgaaggcgat ctcgttaaaa cagtgggcaa acctttccca cgggactccc
     1141 gggcaccgga gaggtggcca tcagggacca ttaccgtcag ggtttatgac gatcagccgt
     1201 ttgatcggca aattgttatt cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga
     1261 ataacaatat ttattcgtca tgccgcctga tagtgaagaa aaacggtgct gaaatttata
     1321 accgaacgac cctggataat acgctgatat atacgggtgt tattgatatg cctgccggtc
     1381 acggtcacat gacgctggag ttttctgtat cggcatggct ggtaaatggc tggtatccca
     1441 cagcaagtat cagcgatttg ctggttgttg tgatgaagaa atccacagca ggtatcagta
     1501 tcagctgaat tttataaccc agatacgggc accagaaatg gtgccttttt tattgcagaa
     1561 aagcgagagg taattatgcg taaactttat gctgccattt tgtccgcagc catctgtctg
     1621 gctgtatccg gtgcgcctgc atgggcgtct gaacatcagt ccacgctgag cgcggggtat
     1681 cttcatgcct cgacgaacgc tcccggcagt gatgatctga acgggattaa cgtgaaatac
     1741 cgttatgagt ttacggacac gctggggctg gtgacgtcat tcagctatgc caacgctgaa
     1801 gatgagcaaa aaacgcatta cagtgatacc cgctggcatg aggattccgt gcgtaaccgc
     1861 tggttcagcg tgatggcggg gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg
     1921 atggcgggcg tggcttacag ccgtgtgtcg accttctccg gggattatct ccgcgtaact
     1981 gacaacaagg ggaaaacgca cgatgtgctg accgggagtg atgacaaccg ccacagcaac
     2041 acgtctctgg cgtggggggc tggcgtgcag tttaacccga ccgaatccgt ggccattgac
     2101 cttgcttatg aaggttccgg cagtggcgac tggcgcactg acggtttcat cgtgggtgtc
     2161 ggttataagt tctgattagc caggtaacac agtgttatga cagcccgctg gttcaggcgg
     2221 gctttttgtg gggtgaatat ggcagtaaag atttcaggtg tactgaaaga tggggcgggt
     2281 aaacctgtcg taaattgtgc gattgaactg cgggccagaa gaaccagtcc gaccgttgtg
     2341 gcacacgttg ttgccacttg cgtgacggac aataacggtg cttatgtgat tgaggctgag
     2401 ccggggtatt acgaggttgc gcttcactgt aacggctggc agccaacccg tgtcggggat
     2461 attgatgtgg caccgactga tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag
     2521 gatggtgatt tacgtccgga ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag
     2581 cagagtgccg gggctgcagc cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc
     2641 gcaacggccc gtgatgatgc acaacgtttt gcggagaaag cccgacagga tgcaaccgtc
     2701 acagctgagg acagaaaggc cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc
     2761 gcagccggac agagcgcaca ggatgccgca ggttatgccc gcgcagcaga acaggccaaa
     2821 aatgacattg atgctgcgct gaccggcact ctgaaaatgg ctaaccatct gtcagaaatc
     2881 gcagcagcag gcgaaaaggc acaacagaag tcccgggata atct
//
LOCUS       DAJTRB010000221         2890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTRB010000221 DAJTRB010000000
VERSION     DAJTRB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.227"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="OO330_004286"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="OO330_004286"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5884895.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="OO330_004287"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="OO330_004287"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5884896.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1134..1892
                     /gene="tam"
                     /locus_tag="OO330_004288"
     CDS             1134..1892
                     /gene="tam"
                     /locus_tag="OO330_004288"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5884897.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMHEVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1896..2810)
                     /locus_tag="OO330_004289"
     CDS             complement(1896..2810)
                     /locus_tag="OO330_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5884898.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          668 c          771 g          691 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca gggtccgact accggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
     1141 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
     1201 tgcttgccag agtgccgctg gagaatgtcg aatatgttgc cgatcttggc tgtggcccag
     1261 gtaacagcac cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcatcgact
     1321 cgtctccggc gatgattgct gaagcgcgca gtgctttgcc agactgccag ttcgtggaag
     1381 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     1441 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
     1501 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     1561 acgaagttgc ttgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     1621 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     1681 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     1741 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     1801 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     1861 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     1921 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     1981 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt
     2041 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     2101 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     2161 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     2221 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
     2281 gaaagctgtc cattgcggcg ctgaaccgcc aaccattctc ccattattaa caagatacgg
     2341 ttagctggag agttcgaagc cagtacacgc tgaagatctc cagtcttgag ataatgagcc
     2401 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     2461 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctctcgc
     2521 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     2581 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     2641 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaatataaca
     2701 gcaatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagccg
     2761 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     2821 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     2881 caaattacct
//
LOCUS       DAJTRB010000222         2831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTRB010000222 DAJTRB010000000
VERSION     DAJTRB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.228"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..189
                     /locus_tag="OO330_004290"
     CDS             <1..189
                     /locus_tag="OO330_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5884899.1"
                     /translation="GKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYG
                     QVIRRDQESALACWGIDQ"
     gene            186..656
                     /locus_tag="OO330_004291"
     CDS             186..656
                     /locus_tag="OO330_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5884900.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(745..1257)
                     /locus_tag="OO330_004292"
     CDS             complement(745..1257)
                     /locus_tag="OO330_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5884901.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1452..2216
                     /locus_tag="OO330_004293"
     CDS             1452..2216
                     /locus_tag="OO330_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884902.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2167..>2831
                     /locus_tag="OO330_004294"
     CDS             2167..>2831
                     /locus_tag="OO330_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5884903.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          789 a          634 c          730 g          678 t
ORIGIN      
        1 ggtaagtgtt tcccgtcgac gttttataag cggctgaatg ccggtgatcg taagggcgca
       61 tgcgaggcga ttcgctggtg gataaaagat gttgggcgcg attgccgcat acgttcaaat
      121 aactgctatg gacaggttat tcgtcgtgac caggaaagcg cattagcctg ttgggggata
      181 gatcaatgag cagagtcacc gcgattatct ccgctctggt tatctgcatc atcgtctgcc
      241 tgtcatgggc tgttaatcat taccgtgata acgccattac ctacaaagag cagcgcgaca
      301 aaaacgccgg agaactgaag ctggcgaacg caaccattac tgacatgcag cagcgccagc
      361 gtgatgctga tgcactcgat gctaaataca cgaaggagtt agctgatgcg aaagctgaaa
      421 atgatgctct tcggcgcaag cttgataatg gtggtcgggt gctcgtcaaa ggaaaatgcc
      481 ctgtgccatc ctcagccgaa acctccagcg cctccggcat gggcaatgat gccaccgtcg
      541 aactctctcc agttgctgga cgaaacgttc tcggtatccg ggacggaatc atcagcgacc
      601 agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg gggtagcggt
      661 aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg gaagctggat
      721 tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc taaaactacc
      781 aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca ctttcactgg
      841 atgaagttgc aacattcctg ccaccaaagg agcaatgtaa cgcagcacgg cgataaagcg
      901 cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc gttctattgt
      961 attggaacgg ctcgatatta tacatgcaag ttgagggata tgcccaatta ttataccaag
     1021 atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc cagcttccca
     1081 tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg atgctggtgg
     1141 caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa gaagccccca
     1201 taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact ccatcaatgt
     1261 ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat ttaggcttaa
     1321 acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt cgcaggagtg
     1381 agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag taaatcacag
     1441 gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac cgggccggag
     1501 tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct atcaggaaga
     1561 gggccaaaaa gcttgattgg gcacgcagtg gcggtacgca ggtttgcaaa aatggtacgc
     1621 aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca caaaaaagta
     1681 cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg cgtaccgatc
     1741 ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt ggttatgccc
     1801 gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg ctcgaggacg
     1861 aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt cggtggttgg
     1921 tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa aatatcagcg
     1981 ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt ggcacgcttg
     2041 cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact gataaggtgt
     2101 ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat ggagagcgtg
     2161 acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt acgtagtttc
     2221 tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc aaaatcatgg
     2281 gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt ttgttgtgcc
     2341 cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat tcagatagac
     2401 aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa ccgggttact
     2461 ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa gtcactggag
     2521 gggatcgata tgttgtggct ggaagaagcc cacgcactga cggaatacca gtggaaaatt
     2581 ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa ccccggactt
     2641 gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac tctcatccgc
     2701 aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt tatcgacgcg
     2761 gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc ggagtctgat
     2821 gatgatgcgg c
//
LOCUS       DAJTRB010000223         2744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTRB010000223 DAJTRB010000000
VERSION     DAJTRB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.229"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..523
                     /locus_tag="OO330_004295"
     CDS             <1..523
                     /locus_tag="OO330_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5884904.1"
                     /translation="HDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKAR
                     SVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENA
                     LRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQ
                     VHELLPWNFSSVK"
     gene            complement(777..941)
                     /locus_tag="OO330_004296"
     CDS             complement(777..941)
                     /locus_tag="OO330_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884905.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YARNAEIPGL"
     gene            1843..2127
                     /locus_tag="OO330_004297"
     CDS             1843..2127
                     /locus_tag="OO330_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5884906.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            2115..2600
                     /locus_tag="OO330_004298"
     CDS             2115..2600
                     /locus_tag="OO330_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5884907.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          720 a          584 c          787 g          653 t
ORIGIN      
        1 ccatgacgag gatgtgcgcc gtccgacaga aatgactcag gaagcgctca gacggatagc
       61 agagttatac gacatagaag cggagatacg tggcagtccg gcagaggaac ggcttgcagt
      121 cagaaaagcc agaagcgtcc agttgatgca gtcgttgtac gactggatac agttgcagag
      181 gaaaacgctg tcgaaacatg cggagatggc gaaggcgttc gactatatcc tgaatcactg
      241 gaatgcgctg aacgagttct gtcgtgacgg ctgggtggaa atagacaaca acatcggtga
      301 aaacgcgtta cgatcggtgg cggttggaag aaaaaattat ctctttttcg gctcagacaa
      361 gggaggagaa agtgcggcga tcatctacag tctgctggtc acctgcaaac agaacgaagt
      421 ggagccggag gactggttgc gcgaagtgat cgagaagctc aatgactggc cgtcgaacca
      481 agtgcatgaa ctgctgccct ggaacttctc gtctgtaaaa taatccttac gctacgtact
      541 tctcggggcg cttaccttca aaatgatgga cattattttt gtggaaccgg aggtaacaga
      601 ccagacggtt taaatgagcc ggttacttta aacgggtgat ggacagcacc tcaaaagttc
      661 agatggatta tatttgtctg caataccccg ggtattttcg atttgcgaaa tggatggaac
      721 gacttgctca ggggatagct gatggagtta ctgaagtacc gaaggaccac taaaaatcac
      781 aggccgggaa tttcggcgtt tctggcgtag gtgtgccacg aatgcatcca gtggcgtgat
      841 gttggatgca atgctgtatg agagatgagg ggcggcgtcc agacgcagcc ttcaaaccgc
      901 cttgagctgc tgccgttaag agcggtggtg gtgagcgtca aggttaaaag gcgcagaaca
      961 tgtgtcaggt aggtgttgcg gagatgaacg aaagtgaact gtcgatgaag tgtcgaaagg
     1021 aacctctagg cgatgtcgaa aatgtgctat ctacgcagcg catgacgagt ccagcagata
     1081 tctggtactg gctgggcggc atccggcata taggcagcat gaacgcaaca caggctctcg
     1141 tacggaacgt gggaacctgt cgtctcgatg ctaagggagc ggcgcggagc agaggcactg
     1201 caagcgcctg agtaccgatg cgagacacag gggcagatgg cttcgtagta gtgaggaagt
     1261 gcctgtaatg ggcatggagc gaagggggtc agtcgttggg gctggtttat cggtcaaccg
     1321 gatagccggg aggagcccat gagtcagcca aagccgttcg aggtctctaa gtatgcggtt
     1381 tggaaagcat accagcgggt caaagctaat cgaggtgcgg caggtgtcga tgggcagtca
     1441 gtcgaggcgt ttgaggttac caatcgaagc tatacccggt aatggcgcag gttgacctgc
     1501 acttggccaa gtggattgcc cgcaaacaca agcgagcgcg aggtagtctg gtgcgtgcat
     1561 tcgagtggtt actgcgaatt cgtcacgact gcccgacgct gtttgcacac tggtgcttag
     1621 cttacgatac ttgaatggtt caacgacaag agccgtatga cgcgagagtg tcacgtacgg
     1681 ctccgtggga gcgtaggggg gcggtgcccc tgcgccaccc gatatcccct ttgagtgcgg
     1741 ttacgtccac agaggtaaat gccgctgacg cggcatctgt aatctgttac tttgtgtata
     1801 tcatttgcta tatattgtca atatgctatt gttgtaaagc aaatgatata cggaggcttt
     1861 atgaaatcag gtgttcagct caatctcaga gcaagagaat cacagcggat actcattgat
     1921 gctgctgcag aaattcttca taagtcgcgt acagatttca ttctggaaat ggcctgtaaa
     1981 gctgctgaag atgttattct cgaccggcgg gtgtttaatt ttaatgacag gcaatatgaa
     2041 gagttcattg aaatgcttga tgctccggtt gcagatgatc ctgccatcga aaaattgctg
     2101 gcaaggaaac ctcaatggga tgtgtaactg caccagaacc tttatcctca tttcatcagg
     2161 tcgctgaatt tgtcagcagt gaagctgtac ttgatgactg gttaaagcag aaggagctaa
     2221 aaaatcaggc aatcggagcc acccggactt ttgttgtatg cagaaaaggt acgcaacaaa
     2281 tagtcggttt ttactcactg gccactggta gtgtcaatca tacagaagct acaggtaacc
     2341 ttcggcgcaa tatgccagac cccattcctg taattatact tgcacgcctg gctgtagatg
     2401 tgtcatttcg aggaaaaggg cttggcgcgg atttacttca tgatgcagtt cgtcgttgtt
     2461 acagggttgc cgaaaatatc ggtgtgcgtg caattatggt tcatgcgctt acggaaaacg
     2521 caaaacagtt ttatattcat catggtttta agccatcaaa aactcaggtg caaacattgt
     2581 ttcttaaact ccctcaatag ggagtaatgc cgatcagtta aggatcagtt gaccgatcca
     2641 gtggctgtgt aagaatccgg aaacgctcac tcgtttccgg atttttctgt gcacagtaaa
     2701 agaccacctc ctgaggaggt ggcttttcag aatgccccct ataa
//
LOCUS       DAJTRB010000224         2695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTRB010000224 DAJTRB010000000
VERSION     DAJTRB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.230"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            144..641
                     /locus_tag="OO330_004299"
                     /pseudo
     CDS             144..641
                     /locus_tag="OO330_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="OO330_004300"
                     /pseudo
     CDS             682..873
                     /locus_tag="OO330_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(967..1176)
                     /locus_tag="OO330_004301"
                     /pseudo
     CDS             complement(967..1176)
                     /locus_tag="OO330_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(1226..1405)
                     /locus_tag="OO330_004302"
                     /pseudo
     CDS             complement(1226..1405)
                     /locus_tag="OO330_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1929..2213
                     /locus_tag="OO330_004303"
     CDS             1929..2213
                     /locus_tag="OO330_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5884908.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHPLPVPR"
     gene            2213..2488
                     /locus_tag="OO330_004304"
     CDS             2213..2488
                     /locus_tag="OO330_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5884909.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          728 a          675 c          682 g          610 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgcaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaaggagat atatcgcagc tctgacgact
      961 cactccttat ttacccggga aatacttctc cagcaatgca tacaggatgg cagcggtttc
     1021 agcatccatt atgccgtcat aatttttctg ccggaaatga agctgaaaag cgcggacaag
     1081 cgccttatat ccgtcatccg tctccacacc agaaatactg tatccatagg tcttcagccg
     1141 tgacacgata tcaaccttat ccggaatgcc ctgcgattca aaaaataaat atttagtcag
     1201 taattttatg tttttcctga aaaaattact ctttttcgaa ctccagtgag cgttcacgac
     1261 tcatcagtgt gaaaatgatg gcaaaaacag cagacagtat cagtacgata aatgtggccc
     1321 cccatcccag ataatcgaca acaagtccca ttcccagtgt cgcaacaatt gagccgaaga
     1381 tataggaaaa tgtgcctgac agccctaaca gaataagttt acgcgtaaat cgcccggaat
     1441 ccagaagagc attaagtatc acataaacag gttccatacc agtcctgacc gcatcaggac
     1501 gccagtaaat aaaatctcgt agtccaccgg catcagagac aagagtaatc gtcgttaccg
     1561 gtatttcgac tggtttgtac tggtgtgagc ggacaaaact actgtacgtc tctcatacac
     1621 gctcgtaagc ggccctcacg gcccgctaac gcggagatac gccccgactg cgggtaaacc
     1681 cttgtcggga ccactccgac cgcgcacaga ggcattttca tcgctgacag gccgcatggc
     1741 ctcgcagagc cggaggggga ctggtgatat tcggacgacc gtaccggctt gcgccgggct
     1801 tcggcggttt tacacctgtg ctacatgtaa ccaccgtatg ccgccttata tgcgctgact
     1861 cgcattattt gccttgttcc gtctgaaagt aatcactatg attaaatatg attaacaact
     1921 aatcgggtat gcaaatgaaa aacaataccg cacaagcaac aaaagtaatt accgcgcatg
     1981 tgccattacc tatggctgat aaagtcgacc agatggccgc cagactggaa cgctcccggg
     2041 gctgggttat caaacaggcg ctttctgcat ggcttgccca agaggaggag cgtaatcgcc
     2101 tgacgctgga agccctggac gatgtgacat ccggacaggt tatcgaccat caggctgtac
     2161 agtcctgggc ggacagcctc agtactgacc atccgttacc ggtgccacgc tgatggaact
     2221 gaagtggacc agtaaggcac tttctgattt ggcgcggtta tatgattttc tggtgctggc
     2281 cagtaaacct gctgccgcca gaacggtgca gtccctgaca caggcgccgg tcattctgtt
     2341 aactcatcca cgtatgggag aacagttgtt tcagtttgaa cccagggagg tcagacggat
     2401 ttttactggc gagtacgaaa tccgttacga acttactggc cagactattt atgtattgcg
     2461 cctgtggcac acacgagaga acaggtagat tgggtgtgga ggcaacttta acatagcctg
     2521 caggtattaa ttctggagca ttgttatcag gccagatatt ttgcattcca ttaaataatc
     2581 ttgtaggaga taaaataaat tacgatgaaa taacgaaaat taccgctcgt gactggcggc
     2641 aatatcgcgc tccgggctgg caaataaccc atcagaaacg ttattgccag acatt
//
LOCUS       DAJTRB010000225         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTRB010000225 DAJTRB010000000
VERSION     DAJTRB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.231"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(64..822)
                     /locus_tag="OO330_004305"
     CDS             complement(64..822)
                     /locus_tag="OO330_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5884910.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO330_004306"
     CDS             complement(830..1933)
                     /locus_tag="OO330_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5884911.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO330_004307"
     CDS             complement(1943..>2572)
                     /locus_tag="OO330_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5884912.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMIMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          682 c          601 g          578 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatgatc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggtggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJTRB010000226         2379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTRB010000226 DAJTRB010000000
VERSION     DAJTRB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.232"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..107
                     /locus_tag="OO330_004308"
     CDS             <1..107
                     /locus_tag="OO330_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5884913.1"
                     /translation="QTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            100..1059
                     /gene="ssuA"
                     /locus_tag="OO330_004309"
     CDS             100..1059
                     /gene="ssuA"
                     /locus_tag="OO330_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5884914.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1056..2201
                     /gene="ssuD"
                     /locus_tag="OO330_004310"
     CDS             1056..2201
                     /gene="ssuD"
                     /locus_tag="OO330_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5884915.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2210..>2379
                     /locus_tag="OO330_004311"
     CDS             2210..>2379
                     /locus_tag="OO330_004311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR5884916.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASG"
BASE COUNT          525 a          650 c          669 g          535 t
ORIGIN      
        1 tgcaaaccca tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
       61 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg
      121 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      181 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      241 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      301 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      361 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      421 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      481 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      541 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
      601 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
      661 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
      721 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
      781 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
      841 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
      901 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
      961 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1021 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1081 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1141 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1201 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1261 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1321 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1381 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1441 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1501 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1561 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     1621 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     1681 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     1741 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     1801 atcttgatga tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag
     1861 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     1921 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     1981 gtcctacggt cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2041 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc
     2101 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2161 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac
     2221 gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2281 ggtgtggcaa ctggcctcct cggttggctg gctgtcgacg cgtattttgc cttcaccgga
     2341 aggagtggtg acggcgttct ggacgctctc cgccagcgg
//
LOCUS       DAJTRB010000227         2201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTRB010000227 DAJTRB010000000
VERSION     DAJTRB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.233"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..105)
                     /locus_tag="OO330_004312"
     CDS             complement(<1..105)
                     /locus_tag="OO330_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5884917.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGV"
     gene            complement(116..2039)
                     /locus_tag="OO330_004313"
                     /pseudo
     CDS             complement(116..2039)
                     /locus_tag="OO330_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          576 a          561 c          475 g          589 t
ORIGIN      
        1 tacgccatta tcaggggtac agataccttt aggagctgca agtgctacac tacttaacga
       61 gagcataaac acagcaaagc accactgttt gatatacttg ttcataaaaa tccttttaat
      121 gacatgtcac accctgttca attaatccgc tctcactttc tggtgtcgaa aatgaataat
      181 tgaagatgca ttttgttgag gcgttttctc cccaggataa cgtaacctgt cctttttctg
      241 gtagaccaga aagataaagt tcgccacggt caccgacaat tcctgaatct gccaaacttg
      301 catcttgtat cgcagcaatg gctccaaaag gtaccggttt accatttgca tttaacactc
      361 tgaagagaac gcgataaccc acatgagtca caaactccgc tttaaccact gcaccacgcg
      421 tgggaaccat cgtaacgacg gtattatcaa gttctatatt cgacttcgag aaatagttat
      481 cgctgattgc tacccgattt tcatcataag ggtttagctg tgtcttgacc gtatagccgc
      541 gccagtccgt cgcagcaccg cgcatattat ctatttctaa cccggcggcc ccaggtgctt
      601 taacaagagc aatagtttca cctaactcct gacttaacgt taccccttcg ctgtgtgcca
      661 gaattccccc tctgatacca taattcagcc tctgggaatt ttggctgtag ttatagctgc
      721 cgttcaccgt gccataagag ccatcatagg tcagggacat attaccgcca acctcatcat
      781 catgactgtt ataggactgc attaaattcc atgaaaggtt atttctttcc agcgccgtac
      841 cgctgagtcc gagttgatgt gagctaccgg aattatcgtt ttgcgaaaca gaataggtgg
      901 cgtaactatt accccaccag cccgataaag gaacagataa agagagcatg tagatctgct
      961 cactggtgtt atcactggca tggttgttcc ccgtcccctg tctggcagta ttggaataac
     1021 tccaggagac gttccacgag agacgcttcc agctactgct gtagccaaat tgcattaatc
     1081 gttcgacatc atcggtatgc cagtattgtt gccggcttaa tgtcaggtat aaattgccat
     1141 aatctctccc caacgactgc gttagcgttc cctccacgcg actacgtcgg ttacctgttt
     1201 cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc attgagtggt atagtagccc
     1261 tcggtggagt aacgatagcc aaccaggcgc aagctagtgt ctgtgtcgtc aaatagtttt
     1321 gaatagagaa agcgatatga ctgacccgtt tctgtatcat catggctaaa attcgcacga
     1381 gcatgtgtca catcgaaaga aattgcgccc aaagcgccaa gatctttacc gacacccaga
     1441 gctgcagaac cataattttc agcagcctgc atacctccat ataaagtgat cccgtgggaa
     1501 aaaccatgaa gtatttgcgc ctgaaggacg tccggtgtaa atccgtcttc atcgcgcaat
     1561 tcacccacgc tgacatcaac atctgtcaga ccttcacgtt taagaatcgc caatgaagca
     1621 taaggttggg tgaatcgttg ttcgctaccg tcttcttctt tgatggttac gtccaaatcg
     1681 ccacctgtac ttgcggtgtt gagatcggtg atctcaaaag caccttgtgg aacggctgac
     1741 tgataaatca cgtagccatt ctgccgaatg atgacggtgg cgttggactt tgcaatccca
     1801 cgaaccacag gagcaaagcc attctggctg gcgggtagca tatcgttatc agtatacaaa
     1861 cgcgcgccgc gaatttgcgt actgtcgaaa atatcgctcg ccgtccacgt atcaccaatc
     1921 ataatctggc ttcttaacgc ggcaatatcg cgctgtatcc aggtactaat ggagttgtat
     1981 tccgcacggc catcgctggt gtttaaagta ctgtaattac gtagccgcca cggtccaata
     2041 ttcagtccat tgcgcagatt gagataactg gtatctgtct cttgtgaacc ataatctgaa
     2101 cgtagtgtat gtgaaccaga aaaatcgtag ttgatggtga gcgcattaat cccctcatcc
     2161 caacgcgtgg ggctgatgta atcataagcc atcgcgtcca t
//
LOCUS       DAJTRB010000228         2142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTRB010000228 DAJTRB010000000
VERSION     DAJTRB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.234"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>2142
                     /locus_tag="OO330_004314"
     rRNA            <1..>2142
                     /locus_tag="OO330_004314"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          587 a          468 c          657 g          430 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tc
//
LOCUS       DAJTRB010000229         2136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTRB010000229 DAJTRB010000000
VERSION     DAJTRB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.235"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..672)
                     /locus_tag="OO330_004315"
     CDS             complement(<1..672)
                     /locus_tag="OO330_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5884918.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNG"
     gene            complement(1271..1519)
                     /locus_tag="OO330_004316"
     CDS             complement(1271..1519)
                     /locus_tag="OO330_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5884919.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1874..>2136)
                     /locus_tag="OO330_004317"
     CDS             complement(1874..>2136)
                     /locus_tag="OO330_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5884920.1"
                     /translation="QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELG
                     FNLVIAEDACSAASAEQHNNSINHIYPRIARVRSVEEILHAL"
BASE COUNT          553 a          395 c          512 g          676 t
ORIGIN      
        1 accgttcctt aaattttccg gcaggtatgg aatatcggtt aacatattgt cgattgccca
       61 tatggagtga atgttgtgag gtagaggggg gagttcacta agaagattcc ctattgcgct
      121 aatctcttgt aaagaaaatg gtaaaggtgg taaaactgcc agcccattac atgatacatc
      181 taatgtttcc agtagttctg gtaaaacagg aagagaacat aattgattat ttgagacatg
      241 aagctccttc aaagatatgg ggagtgtggg tagtgtgatt agttgattgt gggacgcatt
      301 caataattta agtccttgag gcaaagcagg cagttcaata agtctgttat agctgacatt
      361 aagttgtgta agggacgcag gcaatgggga gattaagctt aaattatttt tacttatatt
      421 aattgattta attcccgggg ggatttcagg taatgttgtc aggcctaatt cagacaagtc
      481 caggttcgtc tcttggtttt gtagacatga tactagtcgc tgaaaagcga tgtctcgttg
      541 ttcttcttgt atgcggttat ttttccattc agtccaatgg gttagataat tttcatatgc
      601 gcggctagtg tcgattgaat aagtggagaa cgaatttgaa attaatctgt gattgttatt
      661 tgtcgggagc ataaatatca gggcgtattc tttatgttga gaggtgttgg attctttttt
      721 atctgctttg caatgttatt ggttcccttc agcagtagac aggaacttct ggaggaggcg
      781 gatatatagt ttgtgtaagc atattaactc catatgttat atattgaaga acttctgctt
      841 tacactatcc taaactggat cggtttaatg taaaaaaacg atactttttt ggggggggca
      901 atttttcctt aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg
      961 gtatcagtga atcatgtata taaaaaactg tgttaaggag gctgtccatt agcggttttt
     1021 tattcttctg gaaccgcggc atctttgcgt atctttttgt cttctcaccg tctggtcggt
     1081 gttttgctga gactgctaat ttcctgtctt tgctagtgct gtactggtac tgcccaatca
     1141 tgattggcgg gggcgggcgg agttgagacc gcagacacgt cgtatgcaag aacgtgctgc
     1201 ggctggctgg tgaacttccg atagtgcgag tattgaatga tttccagccg ttactgattt
     1261 tacgtgctaa ttagtgaaca aaccactcgt cagcagactc ccaggtatct ttcagagttt
     1321 cctgaacaaa agttttagct gaatctttat cggcggtgcg cgtaacagaa aggccatcgt
     1381 tgctggtggc ttttacgatc acctctacat cgtcataacg tttactgatg cgtcgggtta
     1441 attcttcctt taacgcatcc acagcaccgg ttggcatttt agtcattttc tctttggcta
     1501 tgcagatttc aaaacgcata aaaatccctc tacactgtgt ttttatacag tattattttt
     1561 aactgtatgg ataaacagtg tcaagagtga gggaaagtga ttttttgggg catgcatggg
     1621 gcaaaaatat ttgttgtggg gcattttggg gcatgattgg gacactttgt tacatatgaa
     1681 ctttgccgat tttcatatga catggcaatc caacgctttg attattctaa agaaaacaca
     1741 tgctcttggg cgttctttag tgatttttaa aaatcccgta tcacgcagtt aaagtggcgg
     1801 gcatactctt caaggctggt gatccccaac cgcacccatt taggatgtga ccattgcggt
     1861 agaccgatgt aaatcataac gcgtgaagga tctcttccac gctacgcaca cgggcgatgc
     1921 gcgggtagat atgattaatg ctgttattgt gttgctcggc gctggcggca ctacaggcgt
     1981 cttcggcaat caccagatta aagccaagtt cccaggcatt gcgggcggtg gattcaacac
     2041 cgatattggt cgagatccca cataacacta ttgtatcgat acctcggcgg cgcagttgca
     2101 actccagatc cgtaccgtaa aacgcacccc actgac
//
LOCUS       DAJTRB010000230         2126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTRB010000230 DAJTRB010000000
VERSION     DAJTRB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.236"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            43..222
                     /locus_tag="OO330_004318"
     CDS             43..222
                     /locus_tag="OO330_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884921.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            274..468
                     /locus_tag="OO330_004319"
     CDS             274..468
                     /locus_tag="OO330_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884922.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1267..1603)
                     /locus_tag="OO330_004320"
                     /pseudo
     CDS             complement(1267..1603)
                     /locus_tag="OO330_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          682 a          437 c          436 g          571 t
ORIGIN      
        1 tagcgtaagg cactacagcg cattgcttta cgagtaataa caatggattt atcaccagaa
       61 gacgcaaagt ttttacgaga actgcaacac cgtatgctca gagaagccat tatcgaacag
      121 caaacgggca tgagcgccat agacccattg cgatacaaac gggcctcatc tcctttagtt
      181 gacgacaatt ccaatgcgga taaaacacgt ccaccacact gattacattt ttcattagca
      241 cagtggtgtg atgcagcata cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg
      301 gagcaactgt tacaggcacg ccaacgggag tttgccacta aatggggacg tgccgcgctg
      361 ccacatgaac gcctaaccta ttttccactt gaagacctca ttaaaaataa tggcaaaaaa
      421 atcaggtctg ataagaaaca cttcttagaa tgcaatgaag acaaataaca acaatctgca
      481 tattgccacc aaccacagat gtggcatcgt tatcagcgca cttgattttc tttatattca
      541 ctgacgagct tgccggggca cctttgtttg ccggtcattc tggtatcatg cagatattta
      601 cggcgtacaa gcccactagc atgacattgt aataaggcct gagcctaacg ctttaatgtg
      661 tgtcatacac tcagtggcgt aatcgttgac aatatggtgc atgtaatcgt aagcgtccag
      721 cgaaatccat ctggtcataa tctgaagcat ccgacaaagt ggtgtccacc aaataagtga
      781 tctgcgccca atatcctgag ccggaacttt gacaaaggcc aacgaaaaat gggagcggat
      841 gttacagagt tctcggtaca ggggaaaagt ggtacctgtc gccagttctc cgatctgttt
      901 aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg tgatggagca tcgttaatac
      961 gatgctatac gatgcattca cagtgcccgg ccagaggatg ccccgtcgct gcatatggat
     1021 cagggttggc aatatcgaat cgcaggctat aggcaaagtt aaagccccat ggagtagcac
     1081 aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatgaagaac ttcttcagca
     1141 cactgctaaa acgcatagtg atcgagcgct gattctggcg aacaactgaa ctaatacatc
     1201 agaatatgca ttatgttaaa taaatatgaa aagatggttt aaataccccg ttacttttga
     1261 cttacactat acggtatcgc atcgtttaat attcgcaccg gccagatttt tatttctatt
     1321 agttgtcaca atactgaatg cgtacgacca cagtattctg gctcctgtgt ggttatgctt
     1381 taattctgcg ttccgggcag ataagcagtt gcttgcagga atccttcttg tgttaatgtc
     1441 agttcccctt tttaccagtg ctgatttcca cattccgtcc aacagagctt atagcctttc
     1501 cctggattat agcattgtcc ggctgaagtt ctttttgaat aataatagaa gcactgctgg
     1561 cagatccagt ccgtttttca taacccactg tactgataac cataatctaa tcagtagaaa
     1621 ttgagtcgaa aataagcact actccataca ggataattag aggtcagttt gattattcac
     1681 aattcatcat cagcattttc tatttctgac gaaatcaaca tgaaaataac catatatgat
     1741 aattattata ataacggctt taattggaat acatatatta caacgtatta tatataattg
     1801 gtattctggg aactatattc tcaaaatata gtagaaacga ggtatgtttc tggtggaaag
     1861 gacagtggga ttaaaaagta agaattgata aaaaaaacgc cagcaacaca tagtgccggc
     1921 ggagggaata ccccatggag aaaatgtgat gcctagagca tcatgaatat ataattaaaa
     1981 atagttagcg ttgtcaatac tttcacaaaa ataattttcg tagtatagaa agatattttt
     2041 atgcatgacc tacctgaatt tgctccgggt agaggttata aataaaaatt gaatcacgac
     2101 aaacacaata ttcatagtat ggcgat
//
LOCUS       DAJTRB010000231         2113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTRB010000231 DAJTRB010000000
VERSION     DAJTRB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.237"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..241
                     /locus_tag="OO330_004321"
     CDS             <1..241
                     /locus_tag="OO330_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884923.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO330_004322"
     CDS             complement(238..1179)
                     /locus_tag="OO330_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5884924.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO330_004323"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO330_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..>2113)
                     /locus_tag="OO330_004324"
     CDS             complement(1684..>2113)
                     /locus_tag="OO330_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884925.1"
                     /translation="LKALLAKLERNATGDNDNKPDDNPTGDGEDDEEETTITGDSAWR
                     AEVIVPGIDLSRKMKPTAFKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFNAVSE
                     VAKGRNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          455 a          588 c          541 g          529 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca accatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agt
//
LOCUS       DAJTRB010000232         2110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTRB010000232 DAJTRB010000000
VERSION     DAJTRB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.238"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..799
                     /locus_tag="OO330_004325"
                     /pseudo
     CDS             1..799
                     /locus_tag="OO330_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="OO330_004326"
                     /pseudo
     CDS             914..1255
                     /locus_tag="OO330_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="OO330_004327"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="OO330_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          520 a          509 c          639 g          442 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaatgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgattaag agacagcagc ttaggggggt
     2101 gcccctatta
//
LOCUS       DAJTRB010000233         2096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTRB010000233 DAJTRB010000000
VERSION     DAJTRB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.239"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OO330_004328"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OO330_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5884926.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="OO330_004329"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="OO330_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5884927.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DAJTRB010000234         2058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTRB010000234 DAJTRB010000000
VERSION     DAJTRB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.240"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..816)
                     /gene="ipaH7.8"
                     /locus_tag="OO330_004330"
     CDS             complement(<1..816)
                     /gene="ipaH7.8"
                     /locus_tag="OO330_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5884928.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGPQIYFSMSDGQQNTLHR"
     gene            1145..1324
                     /locus_tag="OO330_004331"
                     /pseudo
     CDS             1145..1324
                     /locus_tag="OO330_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1341..2031)
                     /locus_tag="OO330_004332"
                     /pseudo
     CDS             complement(1341..2031)
                     /locus_tag="OO330_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          602 a          379 c          467 g          610 t
ORIGIN      
        1 gcgatggagt gtattctgtt gtccgtcact catggagaag taaatctgtg ggccgtggta
       61 gtccggcgaa gaggttaatc tttgcaggga ttgcagaacg cgagtcgaca atggattccc
      121 tgaaatatta atcctgtaac ttgaatctaa ttgtgaaaaa ctctccggaa gagtggttat
      181 ctgattaaaa tgaaaataat attccttttg tctgatattg tttctatcag aaaattgagg
      241 aaaagtaacc agattattgc gctgagcaca gagaatttcc agtttatccg gtaagtcggg
      301 taatgttgtc agcctgttat aataaacatg taaaaatttc aatttttcgg gaagagccgg
      361 taagttatat agcctgttat tttcaacaaa taatgatgtt aaatggtttg gcaaaacagg
      421 aaggttttcc agttgattag aacgaacact taaagttgtt aatgactcag gaagttctgg
      481 tatcacagaa agcctgttat tatcagcatt aagttcttta agaaatgcag gcagctttgg
      541 caatgatgtt aatttattat tttctacaat caaagcagaa atatgctttg gtaattcggg
      601 aagagacgtc aaatttaggt ctgataaatt taatactgct tcttgattct gaaagcactg
      661 ggagagtctg ttaaaagcaa tgcctcgctc ttccccggga gaagagttct tttcccattc
      721 agtcaggatt ctcagataat gttcattact ggttgagttt gagttaatag agggggagca
      781 agaaactgat gagtgtgtat tatttacaga gaacataatg ctaggtcaac cttatatttg
      841 tgagaataaa tatttaaaat tgtatattta tacaaatttc acttaccatt tccgatatta
      901 agttttaata gtttccattt tacacataac agaagttagt tgtaatttat ttatggaggt
      961 tttctatgga attctttttt tattgtgact gcagtcaata ataacactga attcaacatt
     1021 ccagtgagca aggaaatgta atccgagtaa gtatatttgt tttacatcat attttagtca
     1081 agctcattta ttcctattat agaatgacaa attaaaatta ctgtaaaaaa acgatacaca
     1141 tttaatacga gggaggccgg cagaaaaacg gcttgcggtc aggcaggaga aatgtgtgcc
     1201 gcgaatgaag tccttgtatg actggataaa gctccagagg aaaactttgt caaaccatgc
     1261 tgaaatggta aaggcgttca actatatcct gaattactgg aatgcactga atgagttttg
     1321 ccgggtagta cttccaatca ttagaatatg tgcttttcaa tgaaggaacc gatgactttt
     1381 tcgtggattt ccacggaacg cgagaagcag attgttttac gtgccagccg cttgatgcgg
     1441 gggcggaggg tcaggttgtt acgttcatac gttgcgtgaa gattttgccg gtcaggtgtt
     1501 tctcctccgg cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac
     1561 agaacggagt caacagggct aacagttccc gacaggtctc atcattccgt ggcccgcaag
     1621 tgtaagccaa cacaccgcca gttttagtgt tgtacgcata ccagggccag tgttgttgcg
     1681 ccttgctgcc aacgaagcgc cactgctcat caagttcaca gataagggca acatcagcgt
     1741 gagcgactgg cgcagatgtt atccgccggg gcgagagctt tttaatgtcc gtatgacggt
     1801 attgatgccg attttcagtg ttctggcggt ataacagccc cctgcgccgt tatgtgccat
     1861 ttcaacgatc tgctccttaa cgccgagttt tctggcttca taagagtaag tgagctggaa
     1921 aacacgttta catgagcgac aacggaaacg ctcatgccgg gaacaactac ggccatgtcg
     1981 ataaacttca tcggaattac agcgcggaca atgaacagta acggtagtca tggaaaacct
     2041 caaataccag tagattac
//
LOCUS       DAJTRB010000235         2048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTRB010000235 DAJTRB010000000
VERSION     DAJTRB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.241"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..559
                     /locus_tag="OO330_004333"
     CDS             <1..559
                     /locus_tag="OO330_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884929.1"
                     /translation="DVYVPHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIW
                     RKAPPGIDMNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDIT
                     RQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWT
                     TAVTFGIDEIPVLDVSDISVKLQEA"
     gene            561..1166
                     /locus_tag="OO330_004334"
     CDS             561..1166
                     /locus_tag="OO330_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5884930.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1153..>2048
                     /locus_tag="OO330_004335"
     CDS             1153..>2048
                     /locus_tag="OO330_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5884931.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          498 a          472 c          568 g          510 t
ORIGIN      
        1 ggatgtttac gtcccccata atccgaaaaa aacagaacaa aaagccggag tcagtcagta
       61 tccattaaca cccggttcgc tttatgttgg cgtgtacggc ggcagcccgg cagatatcgc
      121 ggcggccatc tggcgtaagg ctccgccggg tattgatatg aatggcaaca caacgttcac
      181 tgttgcagat aaggagtacg atccgccgta tcctgaatac gtgatcagct ggcagacact
      241 caaacccgtc agtctgcatg tcagtgtgac gctgaaaaaa agtgactacc tgccttcaga
      301 tattacccga caggtacagc aatccgtgct ggacgcattt aatggtacag atggtggtct
      361 gcgggcaaga gttgcctctg ttgtctctgc cgggcgctac tatgccggca tttacaaaac
      421 cgatccggaa catattgata ttctgggcct tactgtgagc cgtgatggtt cgtcatggac
      481 aacggctgtc actttcggga tagatgagat tccggttctg gatgtgtcgg atatcagtgt
      541 gaaactccag gaggcataac gtgcagaatg tggctgccac tgtgcttgcg cagtatgctg
      601 ccagcccccg actcaatgcc ctcattaaca gctttaacgc agcgctttcc cccgacagtt
      661 ttatcagtga tttttatggt cttatctgga acatcgatac cgcagaaaag tatggtcttg
      721 atgtctgggg aaagattgtg ggtgtcagtc gctggctgac ggtaaaggac gattttaatt
      781 acctgggctt cagcgagtcc aggatggaca ccccggtaat ggatgatccc tgtccgttta
      841 atcaggcacc gttttacaac ggaaaatcgg atacccggac tgttgacctg tctgatgctg
      901 tataccggcg gctgatactg atgaaagcca tgtcgaacat tactgactgt tccgttccgg
      961 atattaacct gatgctgaga tttatgttcg gaaaaaaacg ccgggcttat gttctgaata
     1021 atggcgggct gaggatgagt tacgtctttg agtccgcgct ctcgttggca gaactggcga
     1081 ttatccagtc gtcgggtgca ctgccatccc cgccgggtgt ttatgtttca gtagttttaa
     1141 aggagtcccg taatgaaggc cagtgataaa ccccgccagc tggcggtccc ctttgcgagt
     1201 accggagata aaaaccgtat cccggacaag gcgacacagc agaccagaga gagcggtaat
     1261 gctgcgtatg attcaggttt tcctccggtg accatgacag cggtctcagc gggaggtata
     1321 ccgccacacg gcaaggattt taacggtctg atgtacgata ttaccgcagc aatacggttc
     1381 gcccaggctg gcggtttgta cacgtataat gccggttttg cgggggccat tggtggatat
     1441 gcaaaaggag ccattctcgc cggagtcgca acaacagcgg tctggctgaa taccacggac
     1501 gataacctga ccgatcctga aggctccgac agtgcgggct gggtaaatct tcttgaggat
     1561 ccgaaaagga tattcctgcg gcagaagaac aatctgtcag accttcagaa taaagggacg
     1621 gcacgggata atcttcaggt ttacagtaaa gagcagtcag atcaacgcta tgttcatcgg
     1681 gaaggcgata aaataaccgg agagctgaaa atccgtggtg ttaatgcgct gaggattttc
     1741 aacgaagctt ttggcctgat ttttcgtcgt tcggaagagt gcctgcacct tattcccacc
     1801 agtgaaggtc agggggaaaa tggcgatatt ggtcccctgc gcccgttcac cattaatttg
     1861 cggacgggtg aaatatccat gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat
     1921 ggtgcgctgg gtatcggcgt tcagaacgcc ctggggggga attcaattgt tcttggtgat
     1981 aatgacaccg gatttaaaca gaatggagac ggtattctgg atgtttatgc taatagtcag
     2041 cgtgtatt
//
LOCUS       DAJTRB010000236         2036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTRB010000236 DAJTRB010000000
VERSION     DAJTRB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.242"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>2036
                     /gene="sat"
                     /locus_tag="OO330_004336"
     CDS             <1..>2036
                     /gene="sat"
                     /locus_tag="OO330_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR5884932.1"
                     /translation="YGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGI
                     ASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQE
                     KITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESI
                     VNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKV
                     ILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLA
                     INNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGH
                     ATEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWK
                     TGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFD
                     FKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIA
                     QNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVT
                     GNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNA
                     IWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          747 a          359 c          401 g          529 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga aatgttctgt
      361 ttaaggataa tttggatttt ggtagcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagggattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa cgaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgcaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
     1261 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
     1321 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
     1381 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
     1441 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
     1501 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
     1561 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
     1621 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
     1681 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
     1741 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
     1801 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
     1861 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
     1921 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
     1981 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gcagat
//
LOCUS       DAJTRB010000237         2034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTRB010000237 DAJTRB010000000
VERSION     DAJTRB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.243"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..364)
                     /locus_tag="OO330_004337"
     CDS             complement(<1..364)
                     /locus_tag="OO330_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5884933.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(431..1645)
                     /locus_tag="OO330_004338"
     CDS             complement(431..1645)
                     /locus_tag="OO330_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5884934.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTLIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          635 a          495 c          411 g          493 t
ORIGIN      
        1 ccagaccgat acgcattaaa ataaagtggc taaaagaatt aaccattcct gttaatgcct
       61 gcattaatga ccagaatatt aagcctgcgc ctaaaactat tcgtgggcca aagcggtcta
      121 gcaaaatacc ggaaggtagt tgtgaaaaac cataagacag agaaaatgca gaaagcaaaa
      181 caccaaactc tgtcgccgat aatcctaact cgccacgaat cgcttcgcct gccacgctca
      241 atgacgaacg gtcgagaaaa ttaacgatcc ccgccataaa taataaaacc agggtcactg
      301 tctgaatacg acgaatccgt tgcggtttat tacccgcaac actggttgac acatcaacgt
      361 ccatttttag ctccttgagc ggaattaatc gatttgatga ctattcagaa aataattaag
      421 cgaaccgtta ttaccagttc aacaatgtgc catcttccag tcgtgcgacg gggagatact
      481 ccgggtcata cggatatttc gccgccagtt tctcatcgaa agaaataccc agccccggtt
      541 catcgcctgg gtgcatataa ccttcctcga agcgccagct gtacgggaaa acttcaagca
      601 tttgttcgga atagcccatg tactcctgaa cgccaaaatt tggtacccat aggtcaaaat
      661 gcagcgccgc cgcatgacaa acaggcgata aatctgaagg gccatgcgaa ccggtacgta
      721 cctgataaag cgatgcaaaa tcggcaatcc gccgcatccc ggtaatgccg cctgcatggg
      781 tgatcgtcgt gcgaatatag ccaatcagct gctcttcgat taactgcttg cagtcccaga
      841 tactgttaaa cacttcaccg accgcaatca gcgtcacggt atgctggcga atcagacgaa
      901 aacattcctg gttctcggca ggcgtcggat cttccatcca gaacaggcgg taatcctcaa
      961 tgctcttccc aaagcgcgcc gcttcgatgg gggtcaggcg atggtgcatg tcatgcaaca
     1021 gatgttcgtt aaaaccgaac ttattacgca ccgcttcgaa taacttcggc gtaaaatcga
     1081 gatacttttc tgaagaccac aactgctctt ctggccacag gcctttggtt gccggttcat
     1141 acgccagtcc tttgcccttc gccaggccat aagtggtttg catacccggt acaccgcact
     1201 gcacacgaat cgccttaaag cccatctgct ggtgctttgc atagtcttca aggacttcat
     1261 caatcgtacg cccggtggtg tggcaataaa ccatcacgcc ttcacgagaa gcaccaccaa
     1321 gcagttgata caatggcata tttgccgctt tagctttgat atcccacaac gccatatcaa
     1381 tggcagaaat ggctgacatc gtgacggggc cacgtcgcca gtaagcacct ttataaaaga
     1441 attgccagat atcttcgata cggctggcat cgcggccaat tagttgtgga caaagatgat
     1501 ctttcagata agaagcgacc gacaattcgc gaccatttaa cgtggcatct cctaatccac
     1561 aaagtccgct ttcagtggtt attttaagcg taacgaaatt acgccctggg caagtaacaa
     1621 aaacatctgc agcaataatt ttcattattg caatctcctg tgttgtaaat agacgtttca
     1681 aacctaattt tacgcctact accatacaag agagaaaaag cgattattca accgtgatca
     1741 cattaaaaac aaaaatttcc accgaggaga aaaaataccc tcaaaaacca cctgaataat
     1801 taaccattat tgttaaaaca acacgttaaa taatcacata acaaactcca ttaatcattg
     1861 aaaatgagca cagaaataga aattaattat attcaattaa ttaaaatgtg tattgcttga
     1921 tgtatttaaa aatattattt tcatggtgag agtggaggta aagaagacac aataataaaa
     1981 aggaatgtga caaataatgg ttctggttgg gcgttaaatt taaggtcacc agaa
//
LOCUS       DAJTRB010000238         2031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTRB010000238 DAJTRB010000000
VERSION     DAJTRB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.244"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..1824)
                     /locus_tag="OO330_004339"
     CDS             complement(<1..1824)
                     /locus_tag="OO330_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5884935.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2031)
                     /locus_tag="OO330_004340"
     CDS             complement(1932..>2031)
                     /locus_tag="OO330_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5884936.1"
                     /translation="RWNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          484 a          580 c          432 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccgccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagcg c
//
LOCUS       DAJTRB010000239         2027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTRB010000239 DAJTRB010000000
VERSION     DAJTRB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.245"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(310..1914)
                     /locus_tag="OO330_004341"
     CDS             complement(310..1914)
                     /locus_tag="OO330_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5884937.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          506 a          500 c          460 g          561 t
ORIGIN      
        1 gttaagcccc tgtagggact agagaccata attaagatga ttgtgtctta ttgctattca
       61 cttatcgact gacccagagc acacaggaag cattcacttt aagtagaacg cttggtgtct
      121 gtaattggtc atcccgcccc tgaaaggatg cgataagtta cggttagttg tcctagaaga
      181 gaaaactttt tgtgtccctt ctccctatag tgagtcgtat taattatcct tgacaagtag
      241 agaaaactat gagtcaaaaa catgaccaca caggtctgta gtaagtgtca tcctagacta
      301 tggtctcctc taggtctcct tataaacatc agccatcaca ttgaccagcc tatcaacctc
      361 gacctcttta gcataacggt cgtacgtaat agaccctgaa ctatgcccca tgatcgcctg
      421 tgcatacgcc aactcgaccc catgttgttt taactgggtc gcaactgtat gtctaaacga
      481 gtgaaacgct aagccgctac ggtctacgag gactttaggc aacaacgtcc gattaaacca
      541 tttactcacc tgctcaccgt aaccgttctt catgagacgc agaccatcga acaccatagc
      601 gtcatcaccg tcagcgcctc tacgggtctc tacaagcgcc ataaagtcag ccaatataaa
      661 accataggcc ccatcgacca gaggcacaca gcggtcacta tgagcgttct tgatgctctt
      721 accgggtaga ctactatcgt cttcattgat atgaatataa acggttcccg cctcagtggt
      781 cctgacgtct ttgacctgta gttgggctat ttcattgagt ctcgccccag tgatagcagc
      841 caaggcggta acataataat gatacggctt accagaggtc ttctgagagt atgccttagc
      901 tgcgaccagt agttgcccca cctgctccgt agagaaggcg ttacgggccc ctgaggcttt
      961 acgctgaggg actttaagct ccagaccctc agtcatgttc tttttaatca agtcattgcg
     1021 cacagcccac ctaaagacag cagccatctt aataagatac ttgttgttga tggtaacgac
     1081 atccagacaa tctgtcttat cttcacggct caacaggtca gcaagtggga catccttgaa
     1141 gcgctgctta cggttcctag gtaactgctg gagcacatca cggaccctaa gcatgtcagc
     1201 cctgttagcg tctgcattga gacccagatg gtcgaatatt tcgattaacg cagcgtgtga
     1261 cgccttgttt tcccttaagg ttgcaggctt ccagttctga tagttctccg cttcgtattg
     1321 ctcaaagagg ctcgctatgg taatgctagg cgtaacttct ggttttactt ctggagcatt
     1381 aacagatagg gacacagagt ctgcttggtt atcttgccta tcaatatcat taaaaagatc
     1441 gacgtaatct atcagaggct gactgtctcc cgcttcgata cgcctcatac aggcttttag
     1501 caccttcagg gcctcattga tgtaacgatg ctggtcaatg cttagcggct cactagccag
     1561 agcatcggta aggctctcac caagttctcc gtattggtcc cggtagatat ccctcatgtc
     1621 aggctcaaag agatctgaac ggcccataga cagttcacac tcagcgatgt ccttaaggtg
     1681 ttccctcatt tcctcgtagg tagcgttggg tctgtctgcg tgtattgcct tgagtgctgc
     1741 ctttatgtgc cttgagtaag ccatagcggt gcgtctatcc ttggtccgta atgatattga
     1801 ggtcattcta tcattgtttg attgtctgac cctaagacgg aagtagtaga cgccgttacg
     1861 ctggtagaga tagtagactg ctggaagttt gtgactactg gatagctgaa tcatatgtct
     1921 gtatgccctg agagggttac acagtgggtt acagtaagta actctgcaag gaggcttaaa
     1981 gccgtatccc ttaggattca gtaggttgga gtactttaag aaaggtc
//
LOCUS       DAJTRB010000240         2004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTRB010000240 DAJTRB010000000
VERSION     DAJTRB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.246"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            214..483
                     /locus_tag="OO330_004342"
                     /pseudo
     CDS             214..483
                     /locus_tag="OO330_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            480..706
                     /locus_tag="OO330_004343"
                     /pseudo
     CDS             480..706
                     /locus_tag="OO330_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            735..1325
                     /gene="ospG"
                     /locus_tag="OO330_004344"
     CDS             735..1325
                     /gene="ospG"
                     /locus_tag="OO330_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5884938.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1664..1948
                     /locus_tag="OO330_004345"
                     /pseudo
     CDS             1664..1948
                     /locus_tag="OO330_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          568 a          436 c          475 g          525 t
ORIGIN      
        1 tcataatcac atcgcataaa actgtcagcg ccagtgatag gacaggtgaa acctgtcaat
       61 acgtaccggc ttacgccggg cttcggcggt tttactcctc tgccatatgc aacaacggag
      121 tgccgcctta catgccgctg gcgcggcata tcctggtgac gatatctgaa cagttatata
      181 ctgtgtatat acgtggtaat gacgaggtgg caaatggcac aggttaatat gagtgtaaga
      241 atcgacgcag aactgaagga tgcttttatg gccgctgcaa aaagcatgga ccgtaatggc
      301 tctcagttaa tccgggattt tatgcgccag accgttgaac ggcagcacaa ttcttggttc
      361 cgtgactagg ttgcggcagg ccgtcagcaa ctcgaatgcg gtgatgtgct tccccatgac
      421 atggtcgaat cctctgctgc tgcatggcgg gatgaaatga gcaggaagat tgctgataaa
      481 tgatggagat gttctggacc atgctggcca tccaggacag gaagcgcatt cgtagtacat
      541 tgcggagcag aacctgatgg ccgccattga accggacgaa cggattggtt attcagccag
      601 cagcctggca gggcagccgt ataaaggccg caacggtcgt gtggaaggaa catctggacc
      661 acacaaggta gcgtgtaacg ttattttgtg tgaaaatctc ttgtgatttt taaatatcgt
      721 aacaaaggta tgacatgaaa ataacatcta ccattattca aacacctttt ccatttgaga
      781 ataataattc tcatgctggc atagtaacgg agcccattct cggtaagtta ataggtcagg
      841 ggtcgacagc agaaatcttt gaagatgtga atgattcatc tgctttgtat aaaaagtatg
      901 atcttattgg caaccagtac aatgagattc tggaaatggc ttggcaagaa tctgagcttt
      961 ttaatgcttt ttatggcgat gaagcatccg ttgttataca gtatggcgga gatgtgtacc
     1021 tccgaatgct gcgcgtgcct gggactcccc ttagtgacat tgatacagct gatatccctg
     1081 ataatataga gagcctttat ctacagttga tatgtaaatt gaatgagttg agtataatcc
     1141 attacgatct taatacaggt aatatgctgt atgataaaga aagtgaaagt ttattcccaa
     1201 tagattttcg caatatttat gctgaatatt acgctgcaac caaaaaagat aaagagatta
     1261 tcgaccgacg attacaaatg cgtacaaatg atttttattc gttattaaac aggaaatatt
     1321 tatagacgta tttgttgatg ctataagcca gaggctcttc tatgagaata tggggaccat
     1381 tccctcgcga tattaacgta atatcttaat tgtatgcacc gccagaatgc gttcagtgcg
     1441 atcacggcag cagacaggta aaaatagcaa caaaccaccc gaaaaaccgc cgcgatcgcg
     1501 tatgataaat tttaaccaca tgcatacctg tgcagcaagg cgaatcacgc acggatttcc
     1561 agccagagac acctgaaacg taaccggctc atttaaaccg tctggtctgt tacctccggt
     1621 tccacaaaaa taatgtccat cattttgaag tggacactat cgtatggaat accggacctg
     1681 aatcactgaa gctttacgcc ttcatttcga agaacattta ccacgggttg tggccggatg
     1741 tcgcctgggc gtaccaaaat caactgtctg tggtatgttc gtgcgcttcc gcaacgctgg
     1801 cctttcatgg cctctgcccg caggtatgtc ggagcaggaa cttgatgccc ttctttacgg
     1861 aagtgcctcc acagtacctg tcgtacttac tgaaagcacc gttatgccca aactcccggt
     1921 ggtaaaaaaa cgtccccggc ggcctaatgc cgatcagtta aggatcagtt gaccgatcca
     1981 gtggctgtgt aagaatccgg aaac
//
LOCUS       DAJTRB010000241         1989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTRB010000241 DAJTRB010000000
VERSION     DAJTRB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.247"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            334..638
                     /locus_tag="OO330_004346"
                     /pseudo
     CDS             334..638
                     /locus_tag="OO330_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            807..>1989
                     /locus_tag="OO330_004347"
     CDS             807..>1989
                     /locus_tag="OO330_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5884939.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGVSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          523 a          397 c          451 g          618 t
ORIGIN      
        1 tacagccatc cttcccgggt ggcaacatac gttatatctg cgacccagac ctgattaggg
       61 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
      121 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      181 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      241 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      301 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      361 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      421 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      481 aggactggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      541 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      601 caagaataaa tagtagtaac tactatagtt gcaaataatg ttaaaaacat ttctatgatc
      661 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      721 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      781 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      841 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      901 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      961 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
     1021 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1081 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1141 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1201 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1261 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1321 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1381 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1441 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1501 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1561 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1621 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1681 aggatatggg aaaactggca tcaatggggg gggtttctgt ttttgaaatc ggtggtgtgg
     1741 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1801 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1861 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1921 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1981 gagccgcat
//
LOCUS       DAJTRB010000242         1941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTRB010000242 DAJTRB010000000
VERSION     DAJTRB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.248"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            29..1426
                     /gene="ccp"
                     /locus_tag="OO330_004348"
     CDS             29..1426
                     /gene="ccp"
                     /locus_tag="OO330_004348"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5884940.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1637..>1941
                     /locus_tag="OO330_004349"
     CDS             1637..>1941
                     /locus_tag="OO330_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5884941.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          538 a          400 c          490 g          513 t
ORIGIN      
        1 aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta ccgcgatcgg
       61 cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt accacgataa
      121 taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata ataaggtttt
      181 aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag aattacccgc
      241 ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc ttggatataa
      301 atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg tttcgcaaag
      361 cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa cgcgttatac
      421 cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac tggcctggat
      481 tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc atcgcaatga
      541 accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg cgttgggctt
      601 tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg ctcattgcca
      661 tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg gtggcgcagt
      721 tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc agttctggga
      781 tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc cgattgaaat
      841 ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc agcttaaagc
      901 gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg atgccattgc
      961 tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt tgcgtggaga
     1021 tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag ataataaatg
     1081 tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt tggggctgaa
     1141 aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga atgtgactaa
     1201 agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg ctttaacggc
     1261 accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac tgatgctgcg
     1321 ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag ctttcctgca
     1381 cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa tttgatcgcc
     1441 cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt aatattatca
     1501 tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt atttaaatta
     1561 agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc cttcaaataa
     1621 atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag aactactcga
     1681 ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat ttccgctgca
     1741 cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc ttgatggcaa
     1801 cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg tccataaatt
     1861 gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc aatccgcagc
     1921 catcgacctg cgttgcgtaa a
//
LOCUS       DAJTRB010000243         1941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTRB010000243 DAJTRB010000000
VERSION     DAJTRB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.249"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..756)
                     /gene="ipaH3"
                     /locus_tag="OO330_004350"
     CDS             complement(<1..756)
                     /gene="ipaH3"
                     /locus_tag="OO330_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5884942.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQ"
     gene            1498..>1941
                     /locus_tag="OO330_004351"
     CDS             1498..>1941
                     /locus_tag="OO330_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR5884943.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDD"
BASE COUNT          552 a          338 c          467 g          584 t
ORIGIN      
        1 ttgcgacaga gactccctga tccgtgagga cagaggattg tcttcgagga tgatagtgca
       61 ggtcggatca aggctaagta tattttccgg aatgtgtgtg atgcgattct cgcggcaccg
      121 gaaaaatatc tcggtttcct ctgagtgatg atttcttaca ggtacggctg gtaggctttc
      181 cagaagatta gtacttacat cgagcgcttc cagtgattca ggtaactcag ggagaaatgt
      241 cagctggtta tttcttactg agagcacttc cagcgatgta ggtaattcag gaagcatggt
      301 tagctgattg ttatctgcat taatatattc cagcaatgca ggcaattcag gaagcatggt
      361 tagttggttg ttatctacat caagatgttt cagagatgcg ggtaattcag gaagtgttga
      421 caggcgattg tcacaggcgt caaggtattc cagcgatgct ggcaattctg gtaatgatat
      481 tagggcattc tgagtaattt ccagaacagt gatttgaggt ggtaagttgt caggtagcga
      541 ggacagattt aaacgattca gttgaagctc actgaactga ttgatgagac attctttaag
      601 tagggagacc gcttcattcc gattttcacc ggggagcgct tgtttttccc atttatccca
      661 tgctgaaaag taatcagcat atgtaccgga aatagtgtta taaaaagaat tttgggacaa
      721 tgaaaagtta ttatttatcg gtaacatgat ggacattcag ttaaagccga tggacaaatg
      781 gctttgttaa aagaaataag taggagtttt tatgaagttc attccatgaa atgaacttca
      841 gcactttttt attataaaag gacaaacaaa attggctgta aaaaaacgat actgtttcag
      901 ttaactgtga atagcttgct tgcaccgtat tgttcttgtt tttcggttag ttaaacatcg
      961 tacttcatat ttgaacgttc tgccggaatg cattatcaat agaggtaaag tcgcaacccc
     1021 aaatcgtaaa ggaaaccgta gcacgtcgta tgcaagaacg tgccacggct ggctgatgga
     1081 tgttcgatag cgcgagtttg aatgaaaatc agccggagat gattttacat aattgctacg
     1141 gaattattca atacaggaat tgcttgcgtc tgcatggatt gacctgaaat attcccgaaa
     1201 atttctctaa aaaactcgaa aaaaatggta actggttgaa tgtattaata tgcaatggta
     1261 cgtgtcaggg attaaaagat gaacgtaaat ttattcaacg cattaatttt aaagggtttt
     1321 attgtttgtt gacgaaaaca ggaatcatgt tcggtctctt tttatctgtt aaaagccaga
     1381 agcatttcct tcgctgactt tatagtcaac cataacacac actctactgt ctgagtccag
     1441 cgttttttaa cattcttgtt aagattatgt gatctttagc gcgggaggaa aatattgatg
     1501 aaacagcctg cgcccgttta tcagagaatt gcgggtcatc aatggcgaca tatctggctt
     1561 tctggcgata tacacggttg tcttgagcag ttgcgccgca aattatggca ttgtcgtttt
     1621 gatccgtggc gagatttact tatctcagtg ggagacgtta tcgatcgtgg gccgcaaagt
     1681 ttacgttgtc tgcagttact ggaacaacat tgggttcgtg cggtaagagg caatcatgaa
     1741 cagatggcga tggatgcgct ggcattccag cagatgtctt tgtggttgat gaatggcggc
     1801 gactggttta ttgcgctggc agataatcaa cagaaacaag cgaaaacggc gctggaaaaa
     1861 tgtcagcatt tgccctttat tcttgaagta cacagtcgca ccggcaagca tgttattgct
     1921 catgccgatt atccagatga t
//
LOCUS       DAJTRB010000244         1901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTRB010000244 DAJTRB010000000
VERSION     DAJTRB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.253"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(43..1692)
                     /locus_tag="OO330_004352"
     CDS             complement(43..1692)
                     /locus_tag="OO330_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5884944.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          446 a          506 c          487 g          462 t
ORIGIN      
        1 attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt cgccgtacaa
       61 accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca acggatactc
      121 gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga tcattttgtg
      181 ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt catcacccag
      241 aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg gtgcccagcc
      301 gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc cgccaggtgt
      361 cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt tcgccatgcc
      421 gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc gtcgccagtc
      481 gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg catcgcgacg
      541 ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca gcgccgagat
      601 gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg ggatgaactg
      661 ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag agtaatccca
      721 accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc gaccagaatg
      781 tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc gatcgtccca
      841 gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg cctgattagg
      901 gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt ggtcgagata
      961 gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg caaaaaccgg
     1021 tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac cgtagttttc
     1081 gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac cactttccgc
     1141 cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac ggccgcccgg
     1201 gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat cctgtggttc
     1261 gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt gcgggtccga
     1321 tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa acttgcgcaa
     1381 gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca gcgggtccat
     1441 tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg actgaacatg
     1501 ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg gcagcccttc
     1561 aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt catacgggtc
     1621 cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt tttgaatttt
     1681 ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg cttttcttta
     1741 agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca cgtttattaa
     1801 cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg gatttggtgt
     1861 tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc a
//
LOCUS       DAJTRB010000245         1826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTRB010000245 DAJTRB010000000
VERSION     DAJTRB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.254"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(208..609)
                     /locus_tag="OO330_004353"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="OO330_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..1826
                     /gene="virF"
                     /locus_tag="OO330_004354"
     CDS             1038..1826
                     /gene="virF"
                     /locus_tag="OO330_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5884945.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          570 a          330 c          340 g          586 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt
     1321 ctgaggagaa aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta
     1381 tcgatttgtt caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag
     1441 cttgcctttt atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt
     1501 cttctcttag tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat
     1561 ggcgtctttc tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat
     1621 tggagagtga aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag
     1681 caaagctttt attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat
     1741 caagcccatc ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat
     1801 tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJTRB010000246         1810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTRB010000246 DAJTRB010000000
VERSION     DAJTRB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.255"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..570
                     /locus_tag="OO330_004355"
     CDS             <1..570
                     /locus_tag="OO330_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5884946.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="OO330_004356"
     CDS             571..936
                     /locus_tag="OO330_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5884947.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="OO330_004357"
     CDS             933..1616
                     /locus_tag="OO330_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5884948.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          505 a          385 c          499 g          421 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgcgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagatta
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc
//
LOCUS       DAJTRB010000247         1808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTRB010000247 DAJTRB010000000
VERSION     DAJTRB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.256"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            173..1198
                     /locus_tag="OO330_004358"
     CDS             173..1198
                     /locus_tag="OO330_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5884949.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1808
                     /locus_tag="OO330_004359"
                     /pseudo
     CDS             1266..1808
                     /locus_tag="OO330_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgtt
//
LOCUS       DAJTRB010000248         1728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTRB010000248 DAJTRB010000000
VERSION     DAJTRB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.258"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(460..606)
                     /locus_tag="OO330_004360"
                     /pseudo
     CDS             complement(460..606)
                     /locus_tag="OO330_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            660..842
                     /locus_tag="OO330_004361"
     CDS             660..842
                     /locus_tag="OO330_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5884950.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(943..1272)
                     /locus_tag="OO330_004362"
     CDS             complement(943..1272)
                     /locus_tag="OO330_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5884951.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRILL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1444..>1728)
                     /locus_tag="OO330_004363"
     CDS             complement(1444..>1728)
                     /locus_tag="OO330_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884952.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQLVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          475 a          400 c          408 g          445 t
ORIGIN      
        1 ggaaaggctg ggccacatcc ctccggcaga agcagaaaaa gcttattatg cttccatcgg
       61 aaacgatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa cccggggcgg
      121 ttcaaatagc aatgtctgtc gcacaacaac cacaatgtca caaataaaaa atgagaataa
      181 ccagttccct gaaggcgacg tagcccggta caagagtaac ccacgttagc gcttggcgac
      241 ggcggaacaa agctgattcg cgacgtaaga aagtggcagg ctcatatgac ggacctctgt
      301 aatgcggtat ccaacccgcg aatatcagca tgattcaccg tcggatttac tgctacgtcg
      361 ccctcaggga attaaaccta aaaagaaaat cgtcaaaatt agagattccg gtttgacacg
      421 gaaagtcata tcagcgtcaa cggagcaccg tattgacgct tatttattgg tgagaactac
      481 gttccatggc aggagttcgt caacacggtt ggagggccat tcaggcagta tgctcagaat
      541 atggcgcaga tacgcttccg gatcgatacc gttcagacgg caggtgcctg cttttccggc
      601 aacaagcagg aatctcagcg ctgaagcagg aattagaggt aaaaacgccc taccgggcca
      661 tgaatcaccc ggtcattgga gtagtaacca aagcagattt agccagcatg gaacaaatat
      721 ctttggtgaa aagttggtta cgggaagctg gagcgcacaa cgtgttagta accagtgcag
      781 ttaataacaa tggagttaca gaactttttg ccttgctgca tacagaagaa ggctgttgtt
      841 aatttcgatt ggttctcaga tgagaatgat gacggtgata cacagataag aatcaatgag
      901 caatgagggt tgccgggcaa ccctcattga ataaaacggg aattacttcg cttcgccgtt
      961 tttcatttcg cccatagctt tcagcttttt ggagatatcg cggcgttctt tggagagctc
     1021 ggcatttttg atgatgtaat cgtcaacgcg atcttcatag tcacctttca tgctggcgat
     1081 gatgccctgg attgcttcaa cgctcatccc tggcttgatg taatcgctca ggttgtccag
     1141 caacaggatg cgcttctggt tgtcacggat ctttttctca acgtcctgaa tttcacgttg
     1201 cagtttgttc ttacgacgga acagacgaac aaattccagt acgtcctgga atgaaggctt
     1261 ggtagtttcc atttttatac ccctgataat gtgagagtcg gattcgttta agcaaccgct
     1321 attcgttagg gccaacatta ctcatagctg ccgcagatga caatgctttt atcccttttc
     1381 actatcatac cctttatcct tgctgaatcg aagcagcagc aagatgattc tgaagttcag
     1441 gaattatttg cgcaaggccc ggcaaatcag attcgatagc aactcaagct gatgtgccgt
     1501 ttccatgttc agccacacat aaccatagat tggtgtttcc acgactttca ggtcatggtg
     1561 gttatcgagc aactgacgca gctcttccac agcatcgttc aatttttccg tattggcaaa
     1621 aaccagctgc ggattacctt cgtacagcgc atgaacaagg ctcagcagta tttgctgcat
     1681 catgtgctgg gtatcacaaa gtttttgcgc gttcaataac acgaaatg
//
LOCUS       DAJTRB010000249         1612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTRB010000249 DAJTRB010000000
VERSION     DAJTRB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.259"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(110..577)
                     /locus_tag="OO330_004364"
     CDS             complement(110..577)
                     /locus_tag="OO330_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5884953.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO330_004365"
     CDS             complement(643..882)
                     /locus_tag="OO330_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5884954.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..>1612)
                     /locus_tag="OO330_004366"
     CDS             complement(1017..>1612)
                     /locus_tag="OO330_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5884955.1"
                     /translation="RFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLLLQGE
                     SDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRYLGKV
                     EKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTDKNIE
                     WYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          516 a          356 c          292 g          448 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gt
//
LOCUS       DAJTRB010000250         1597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTRB010000250 DAJTRB010000000
VERSION     DAJTRB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.260"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..636)
                     /locus_tag="OO330_004367"
     CDS             complement(<1..636)
                     /locus_tag="OO330_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5884956.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="OO330_004368"
     CDS             complement(750..1322)
                     /locus_tag="OO330_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5884957.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          476 a          337 c          317 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacaactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTRB010000251         1549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTRB010000251 DAJTRB010000000
VERSION     DAJTRB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.261"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            351..476
                     /locus_tag="OO330_004369"
     CDS             351..476
                     /locus_tag="OO330_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR5884958.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="OO330_004370"
     CDS             complement(921..1397)
                     /locus_tag="OO330_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5884959.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          358 a          405 c          385 g          401 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggt gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcctc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcaccct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAJTRB010000252         1493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTRB010000252 DAJTRB010000000
VERSION     DAJTRB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.262"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..384)
                     /locus_tag="OO330_004371"
                     /pseudo
     CDS             complement(1..384)
                     /locus_tag="OO330_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            570..1412
                     /locus_tag="OO330_004372"
     CDS             570..1412
                     /locus_tag="OO330_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5884960.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
BASE COUNT          426 a          280 c          325 g          462 t
ORIGIN      
        1 ccgaacacat ggcagagagt ggccacagga taacgcgccc tgagtttccc gattatcgaa
       61 aactgttcag ggagtctgac atcaagagcg cggtagcctt tttaatattt cattttccat
      121 ttcaatacgt tgtagctttt tcctgagctc acggatttca atttgttccg gggtaatggg
      181 ggaggctttt ggtgtttttc cctgccgctc atcacgtaat tgtttcaccc gtcgcgtcat
      241 tgtggaaagg ccgacatcca tagcgctggc tgcatctgcc acggtgtatt tctggtcaac
      301 gaccagttga gcggattcgc gtttaaactc tgcgctgaaa tttctttttt cattatggca
      361 cctgtgttgt tctgaggtga gcatatcacc tctgttcagg tagctaaatt tagtgtgcca
      421 cctcaaggtt cttagtgagc cgtttttaaa agatatgata atatattatt gtttataaat
      481 tgttgatgtt tgatttagta gtatataaag ttcaccacct ttattaggct ttccttttat
      541 ctctacttac tgggtgccag aaactttaaa ttttaaacgg agggggcatt ttgtttaaag
      601 caaatcatgt tccggtatta atgtatcatc atgtcagcca ttgcccagga ctggttacac
      661 tttctcccga gacattccgg aaacaaatga aatggttggc tgaaaacaac tggaaaacac
      721 tgtcatcaga tgaactggag tttttttatc ggggcggaaa acttccccgt aaaagcgtta
      781 tgttgacatt tgatgatgga taccttgaca actggtttca ggtatatcct ttattaaacg
      841 agtttaatct caaggcacat atttttttaa ttaccagttt tattggaaat ggtccggttc
      901 gtcactctcc tggaaaggaa tattctcatc gcgattgcga acatcaaata gcaaccggga
      961 atgctgataa tgttatgctt cgctggtctg aagtgaacga aatgttgcaa tctggtctgg
     1021 tcgaatttca tgttcataca catactcata cacgttggga taaaaagttc acttcacgag
     1081 aagaacagtg taaacatctt cggcaggact tattgagcgg gagagaatat ttaaaaaaaa
     1141 tgacaggaaa atgcagtaaa catttgtgtt ggccggaagg ttattataac aaggattata
     1201 ttcagatcgc tgaagaactt ggattccatt atttatatac aacagaaaga agaatgaatg
     1261 ctcctgctaa aggaactaca cggataggaa gaataagcac caaggaacgg gaaaattgcg
     1321 tatggctgaa aagacgactt ttttattata caacaccgtt tttttcatcg ttgctggccc
     1381 ttcataaggg gccaagatta ccggatgact aataatgaat atactattta cggaatcatc
     1441 accgaatata ggtggtcagg aattacaggc tgttgctcaa atgaaggccc tga
//
LOCUS       DAJTRB010000253         1489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTRB010000253 DAJTRB010000000
VERSION     DAJTRB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.263"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..263)
                     /locus_tag="OO330_004373"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO330_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO330_004374"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO330_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5884961.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1489)
                     /locus_tag="OO330_004375"
                     /pseudo
     CDS             complement(1175..1489)
                     /locus_tag="OO330_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          416 a          375 c          260 g          438 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatctt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctc
//
LOCUS       DAJTRB010000254         1482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTRB010000254 DAJTRB010000000
VERSION     DAJTRB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.264"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..836)
                     /locus_tag="OO330_004376"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="OO330_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          381 a          354 c          359 g          388 t
ORIGIN      
        1 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
       61 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      121 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      181 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      241 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcactagg
      301 aaaacgaagc cgtttttggt gatttggtac attgggcgtt tttcgccttt ggtgtcggtg
      361 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      541 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      601 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
      661 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
      721 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
      781 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      841 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac
      901 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
      961 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1021 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1081 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1141 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1201 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1261 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcagc
     1321 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1381 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1441 tctcattgat gagctgacaa tctctaaaag caatgaggct gc
//
LOCUS       DAJTRB010000255         1479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTRB010000255 DAJTRB010000000
VERSION     DAJTRB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.265"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..138)
                     /locus_tag="OO330_004377"
     CDS             complement(<1..138)
                     /locus_tag="OO330_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5884962.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EH"
     gene            complement(135..491)
                     /locus_tag="OO330_004378"
                     /pseudo
     CDS             complement(135..491)
                     /locus_tag="OO330_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            571..>1479
                     /locus_tag="OO330_004379"
     CDS             571..>1479
                     /locus_tag="OO330_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5884963.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          396 a          336 c          365 g          382 t
ORIGIN      
        1 atgctccagg aagtccatat aactccattc ctggtctact gcctgttgtg acagcgcagg
       61 cgctgcgctt ataaggcttt ccagttgcaa ctgcccggcg agcgccatca gtcgttgatg
      121 ttgcagttcc atcatcacgc cactcctctg cagaatgagt cgtagatgga gagtggatga
      181 tgctgggggg ggttgtcgaa gctcaccaga ttttcactag gatgcacgtc atactctttt
      241 ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc acagggacgg
      301 gcctggattg tttcatgctt tcgttggtta gcaacatcgt gcagccagcg cagaccgtgg
      361 cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt cattagtggg
      421 atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt agtctgtgcc
      481 ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg atctactgat
      541 ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg tcagaatttc
      601 ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat ggatgccaca
      661 atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt tttgccgggt
      721 aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa tgaggcgctc
      781 catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg gcagttatct
      841 ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca tgttcttcgt
      901 gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt tccttctgaa
      961 aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca atccctgccc
     1021 cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg gtttcaccat
     1081 attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca gtattgcctc
     1141 gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa aacaccggaa
     1201 tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg gcatttttac
     1261 acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca gagtggttta
     1321 aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat cttcagcagt
     1381 acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat gcagatagag
     1441 caaaactttc gtgatgaaaa gaacgggcgc ttcggattt
//
LOCUS       DAJTRB010000256         1438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTRB010000256 DAJTRB010000000
VERSION     DAJTRB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.266"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(40..116)
                     /locus_tag="OO330_004380"
     tRNA            complement(40..116)
                     /locus_tag="OO330_004380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Arg,seq:tct)
     gene            complement(118..193)
                     /locus_tag="OO330_004381"
     tRNA            complement(118..193)
                     /locus_tag="OO330_004381"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Ile,seq:cat)
     gene            complement(370..1059)
                     /locus_tag="OO330_004382"
     CDS             complement(370..1059)
                     /locus_tag="OO330_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5884964.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1056..1415)
                     /locus_tag="OO330_004383"
     CDS             complement(1056..1415)
                     /locus_tag="OO330_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5884965.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
BASE COUNT          347 a          402 c          306 g          383 t
ORIGIN      
        1 tacaagtcag ttgctctgcc tgctgagcta agtcggcgct ggcccaccac cgaggactcg
       61 aacctcggac cgtcaactta gaaggttgat gctctatccg gatgagctag tggtggttgg
      121 tggcccttgc tggacttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact
      181 gagctaaagg gccggaggca gaataataac catatgtcat cacatctgca aactcatctg
      241 accaccagca cgtttaacgt cctgtgccat ttttcaggca taaaaaaacc gctcggcggc
      301 gggttttgct tactttacca tcgcgtacaa aatcggcaaa gtatcagatt tgcatgagat
      361 atatgccttt taatctactt ttgcaatact ttgctttgaa aatgccgctt tttgttctga
      421 acgtgttctc atcacaaaca ataaagcctc actatccagt cggtgaaaaa tgtgtttcat
      481 tacaacccag tgaccagtaa atgttttgga ccagtttttg gttgttactc ccgccagtaa
      541 tgccagctcc tgatattcat aaccttcccc accaaaaagt tctgctttta ccgcctgcgc
      601 cgccagccag attaattttt tcaggcgttc ctgtgttttc tctgcaattt tcctagtgcc
      661 agaatgagcc ttaaactctt cccacgccca gtgggttatg gtgatctgat actcccagca
      721 aatacttccg ctgtaacacc acaacaacca ggctttatag tgctcttcaa gagacagaac
      781 agcccgccgc cacgatgatg tcgaaaacgc aacctgactg accagaggaa ttgacgcccc
      841 cttcgccagt gattgttttc ccgggattgg tggattatcc cgcgttatca tttttccggt
      901 cacctcatcg cggtaccgga tttttttacg cctgtaacgc cctgtatcga acatggcatt
      961 ctcctgccag gcttcaagct gaccttttgt cgccccactc aaatcagcgg tggcgataat
     1021 gagctgctca cgaacaaact gtaaatactg gttattcatg cacactccag ctctgtgatt
     1081 tttatcccca accgaccacc aggaacaggc agtccgcgca caatattggt ttcatcaaac
     1141 tgctcgtcgt cgataagcaa ccccgcatgt gtcagtgcat ccagtggtgc tttcaggata
     1201 ttgtccaggt cacgacgccg cttatccggt ggctctgcaa taatctttat cgccagtctt
     1261 ccggacaggc ttaatttcag tcgctgctga cggacaataa gcgccacatc ccggcgataa
     1321 cgctcaccgg cttttgatac aaaatatgtg ctgccacgac gtcgccagta agtattcacc
     1381 gtcggcggat aaggtaaaat aaactcatgg cgcatcagcg cagcacctcc tgcaccag
//
LOCUS       DAJTRB010000257         1409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTRB010000257 DAJTRB010000000
VERSION     DAJTRB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.267"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            443..1192
                     /locus_tag="OO330_004384"
     CDS             443..1192
                     /locus_tag="OO330_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5884966.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          395 a          288 c          292 g          434 t
ORIGIN      
        1 gagtggtgga ttatcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctgggtgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcacacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gagaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcggaaagg
     1321 ccctttccct tcttgtcggt aatttcacta ggctgtgtcc cttaattacg taagcgttga
     1381 taaaagagtc ggatgcagcc cagtttcac
//
LOCUS       DAJTRB010000258         1312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTRB010000258 DAJTRB010000000
VERSION     DAJTRB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.268"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..493)
                     /locus_tag="OO330_004385"
     CDS             complement(<1..493)
                     /locus_tag="OO330_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5884967.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO330_004386"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO330_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5884968.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
BASE COUNT          378 a          276 c          279 g          379 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt ta
//
LOCUS       DAJTRB010000259         1305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTRB010000259 DAJTRB010000000
VERSION     DAJTRB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.269"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..471)
                     /locus_tag="OO330_004387"
     CDS             complement(<1..471)
                     /locus_tag="OO330_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5884969.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
     gene            complement(458..829)
                     /locus_tag="OO330_004388"
     CDS             complement(458..829)
                     /locus_tag="OO330_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5884970.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(841..1242)
                     /locus_tag="OO330_004389"
     CDS             complement(841..1242)
                     /locus_tag="OO330_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5884971.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          297 a          404 c          289 g          315 t
ORIGIN      
        1 cgggattttc actacctcca caggaagacg attttttctt agcctgccct gatggtcatg
       61 ccctgttgca aaacgcgctt caggaagaca cctcagaaca tgccagcggc cattcgccag
      121 ttgctgaaca aatgcccccc ggaaaaagta ttttcccact ctcagtcctt caccagcacg
      181 ccgccgtgtt gtgttcagtt tgatggcggg cagattgccc cggttaacgc ggatcctggc
      241 cgtcattttt cctgacggac tggctttaaa cacccggaca cgctgacgta ccagtttcag
      301 ggggatccct ttcacctggt tatcgcccgc aacggtattc ccggcaacct gccgggtggc
      361 aaccgagacc gctttctgtg ccacacggtt tatcgcccat gcgctggcct gtggcaccat
      421 acgggtatca aggctgttca gattgcggat ggcattctca agccccttca taatatcgtc
      481 ctgcccttac gtttcccggc agcccctcac gccgcaaccg gatataacta catccgccat
      541 catcaggggt gatacgatcc acacagaaag actcacgacc aatcatcagc gaatcattgc
      601 gacacagccg taaaatatct gctgttttca caaacaggga tggtaaagag tcttcaatac
      661 gcacaccgcc ggccaccaga gaaatatttt caggatcatc aaaaaccccg gaaatcatga
      721 tcccttttag ccggcctgac gtaatgaccg ctctaatccc catattgcga ataatggcct
      781 tgtctgcgag atcaagtgca acatcaaaca gattatcgaa atcagccacg ctgcctcccg
      841 ttattgcatt ctggccaggc cacgttctgt cattccggct gccacaccgg caaagacacg
      901 gaacgccatt cccggcggca caaatgccag aggttcatcc cgcgtggcat gaagtgcatc
      961 ggtatgcagc gtcaccagtg ccacgactgt aaccagatca tccatattct gaatcacggt
     1021 ctccggctgc gctgatatca cctcattttc atgtccggtc agcgcatttt ccgggtgggc
     1081 agacttatcc cggatgtcag tgtcatccat attggcaagt tcctcctcca gctcagccac
     1141 acgctgcgca atatctgctg cgcttcccga gatatccggc tcacgtccca gcgatgctgc
     1201 cagttctctc agacgtttca ggttatcgtc tttctttgcc atatctgttc ctgtccgaat
     1261 aaaaaacggg agcccgccgt tactttattc gtacccctta taatg
//
LOCUS       DAJTRB010000260         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTRB010000260 DAJTRB010000000
VERSION     DAJTRB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.270"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO330_004390"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO330_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5884972.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          360 a          229 c          284 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag aactttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAJTRB010000261         1270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTRB010000261 DAJTRB010000000
VERSION     DAJTRB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.271"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            206..457
                     /locus_tag="OO330_004391"
     CDS             206..457
                     /locus_tag="OO330_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884973.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            524..802
                     /locus_tag="OO330_004392"
                     /pseudo
     CDS             524..802
                     /locus_tag="OO330_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            804..>1270
                     /locus_tag="OO330_004393"
     CDS             804..>1270
                     /locus_tag="OO330_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884974.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGT"
BASE COUNT          314 a          319 c          323 g          314 t
ORIGIN      
        1 gtgagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcactaaacc
       61 cgcttcggcg ggtttttgtt tttattttca acgcatttga agttctggat ggtgccggaa
      121 tagaatcaaa aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga
      181 gtgctactta tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg
      241 tgaagatggc ctctatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat
      301 tgtgtgtttg caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga
      361 cggatttatc ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatcgg
      421 aataaaagct ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg
      481 gctgaactcc cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa
      541 atattacaat catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc
      601 atttaagtca gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat
      661 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
      721 ctagataatc tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt
      781 ccagcaacgg atctggaggt aacgtgcggg tattacttcg acctgttctg gttccggaac
      841 tcgggctggt ggtccttaag cccggtcgtg aatccatgcc ggtattccac aatacccggg
      901 tactggtgga gccggaaccg aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg
      961 ttcgccagcc gctagtggaa gacaaaacat tgctgccgtt tttcagtaac gcacgggtga
     1021 ttcgtgctgc tggtggtgct ggcgcattgt ctgactggct gttgcgccat attaaatcct
     1081 gccagtggcc acacggcgat tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg
     1141 gcgcaatggt attgtgctgg cactgcgaca accagctgcg tgaccagaca tccgaatcac
     1201 tcgagcaact tgctcatcaa aacctgtcag catggatgat tgacgtcatc ggtcacgcaa
     1261 taagcggtac
//
LOCUS       DAJTRB010000262         1261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTRB010000262 DAJTRB010000000
VERSION     DAJTRB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.272"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(41..100)
                     /locus_tag="OO330_004394"
     CDS             complement(41..100)
                     /locus_tag="OO330_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5884975.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="OO330_004395"
     CDS             complement(339..1247)
                     /locus_tag="OO330_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884976.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTRSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          249 a          343 c          307 g          362 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccccttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atctcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 t
//
LOCUS       DAJTRB010000263         1163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTRB010000263 DAJTRB010000000
VERSION     DAJTRB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.273"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..721)
                     /locus_tag="OO330_004396"
     CDS             complement(<1..721)
                     /locus_tag="OO330_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5884977.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTET"
BASE COUNT          288 a          294 c          281 g          300 t
ORIGIN      
        1 cggtttcggt gaattcgaca ttgccgaatt tgtcgaagtg aacaaccaga gtgcgaccat
       61 cacccggaat catcttgtca gcaggtgggg tgttattctg gcgcagttcg gcctccatgc
      121 ggtcgaactc agcaatgtag gcttccttga atgcggcggc ttttttgcca gtgaagccca
      181 tcaccaggaa aacgaagccg tttttggtga tttggtacat tgggcgtttt tcgcctttgg
      241 cgtcggtata ggtgacgggc ttaaaattaa gccggttaaa ctccggcgag cattcgagag
      301 tttcgatctt ctgaataacg tttttgtgca ttttccggaa gaactccgca accgcaacag
      361 acgtagtgac agcgcgacca ttttcgatgg ttgcgtcagg gtgagaaagg gtaaggatag
      421 tagccatgat ggcagcctcg atagtcagtt taaatgactc accaccagag gtagcaatct
      481 catgggtggt gagacgcaca gggttgctac aaccggtgac taccgaaacc ggccagcctt
      541 gcggctgccc tgcacgcccc accataatct gaatgtggct gtgcttgacg cataaaaaaa
      601 ccgcctgagc gcggttatgc gcagtagtca tcgtcggggt agcaatcccg gcacccgttt
      661 tatgaggtgc aggtgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      721 ttttctcttt cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      781 gtgacgttaa ccagttaaca attacagttg gctttcccac ccaaaccaat aaggactaac
      841 atgactttta actgtgccgc gtgcgcatag ccgcattcac cgcatcacaa aattcacttt
      901 aaaaagggcg gacatcagcc agcaattaaa ccgatgccgc caactggtac ttcacacagc
      961 aatgtcgtta tttacaaccg gaagcgcact cccaccattt aaatttcaca gacaagaccg
     1021 actctttatg gataccggaa atgcgccttc gtgttgtgcc cggttttatt tcaccacttc
     1081 cgggctttgg tggtatcttt actgaagttc tcacacaacc agtaaggaaa tgaatatgcc
     1141 aacgtatctc gccagagtag aac
//
LOCUS       DAJTRB010000264         1161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTRB010000264 DAJTRB010000000
VERSION     DAJTRB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.274"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            492..914
                     /locus_tag="OO330_004397"
     CDS             492..914
                     /locus_tag="OO330_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5884978.1"
                     /translation="MRQTEDKQHQNIEPEEGTPCNQTGEDNCPDCGAVMGDATYQETF
                     DEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGL
                     CANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            892..>1161
                     /locus_tag="OO330_004398"
     CDS             892..>1161
                     /locus_tag="OO330_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5884979.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHH"
BASE COUNT          371 a          270 c          296 g          224 t
ORIGIN      
        1 cgattggtat cgaggtcatc cccgcgcacg tcactgaata tctgaacaag gtactgactg
       61 aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt cgctcctctg
      121 ccccgatgcc gcaacgtgta acagaagaag gaaaacagga tgatgaagaa aaaacgcagc
      181 catctggcgc aatggcagat gaacaggcaa cggctgaaac aatggaaccg gatgcaactg
      241 aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct gttgatgaga
      301 aatatcagga actgcgggaa gaactccatg aagcccggaa aaacattccg cccaaaaatc
      361 ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa gggattagcg
      421 acccgaatga tccgaaatgg attcccggtc atcatatctc ctcaaatgaa gtcaaaaaaa
      481 cggaaaatga agtgcgacag actgaagata aacagcacca gaacatcgaa ccggaggagg
      541 gaacaccctg caatcagact ggcgaggata actgccctga ctgtggcgcg gtgatgggag
      601 acgcaactta tcaggaaact tttgacgaaa aaaaaccaga tgaagcacag gaagaagagc
      661 cgaagaaaac ggaaaaagct gatgatttgt taccggagaa cgctggcagc gatcagcata
      721 atgatagcaa taatgaaact ggcgaagatg aactcaactg gaaaaaacag gtgttgattg
      781 cggcggttta cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct gcaatccctg
      841 acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt gatgagcgct
      901 tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca gcagatcgtt
      961 cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc tcagcacctg
     1021 tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca ggcaatcagc
     1081 cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat ccggtacatg
     1141 gctgaaacca ttgcccacca c
//
LOCUS       DAJTRB010000265         1157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTRB010000265 DAJTRB010000000
VERSION     DAJTRB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.275"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..1132
                     /locus_tag="OO330_004399"
     CDS             join(<1..750,750..1132)
                     /locus_tag="OO330_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5884980.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     TAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLSFRYLDHKTGETATETLTQRELVA
                     RLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLS
                     RDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          266 a          286 c          382 g          223 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatgcgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt
      961 ggcgaaagtg tgctatgcac aaatggtgaa acagttcctg agtcgtgacc cgttcgaatg
     1021 cgtgctgtgt ggcggccgga tggtataccg ccgggccatc gcgggactga atgtggaggg
     1081 gctgaagaaa aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct
     1141 aagtgtgccc gggaatc
//
LOCUS       DAJTRB010000266         1140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTRB010000266 DAJTRB010000000
VERSION     DAJTRB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.276"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..730
                     /locus_tag="OO330_004400"
     CDS             <1..730
                     /locus_tag="OO330_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5884981.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="OO330_004401"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="OO330_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAJTRB010000267         1110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTRB010000267 DAJTRB010000000
VERSION     DAJTRB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.277"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            69..>1110
                     /locus_tag="OO330_004402"
     CDS             69..>1110
                     /locus_tag="OO330_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5884982.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIE"
BASE COUNT          303 a          280 c          269 g          258 t
ORIGIN      
        1 gaatgaagaa cctgggacat ttattcatca ctcgcagtaa ttttaatctg agttgaggtt
       61 aaaaaacaat gaacaccgat aaacaagttt acccactgta ttacgaagca aaaaatgaca
      121 aagtaagaaa acgtctcggt attaaaggcg gtttttactg ggctgaagcg aaaaaattat
      181 ccattgccat ctcccgtggt gctgttgcga ttgacgatgc aggctacgat gaagatgact
      241 ttaaaaaacc tgttcgcgtc aatttgcccg ttgttgatga cctcccgcca gaaggcgtat
      301 ttgatacgga attctgcaac cgttacgaaa aaggcgggga agatggcatc acaatggtat
      361 ttatcgcatc ttctccctct gttcaggaca aaccggccag cactgacaat accaacgtca
      421 acggcgaaga catgacagaa attgaggaga acatgctcct gccagtttcc ggtcaggaac
      481 tgcccattcg ctggcttgcg caacacggca gcgaaaaacc agtaacgcac gtttcacgcg
      541 acgaactcca ggcattacac attgctcggg atgaagaact accagctgtt actgccctgg
      601 ctgtttccca caaaaccagc ctgctcgatc cgctggaaat tcgcgatctc cacaaactgg
      661 tgcgtgatac tgacagagtt ttccctaatc caggcaattc aagcctgggg ctgatgactg
      721 cttttttcga agcatacatg gacgcagact acaccgatcg cggtctgctg acaaaagagt
      781 ggatgaaagg aaatcgtgtt tcacgcatca cgcgcacggc ttccggcgct aatgctggcg
      841 gcgggaacct caccgatcgc ggcgaaggtt tcgttcacga tctgacgtca ctggcgcgcg
      901 acgtagccac tggcgtactg gctcgttcaa tggacgtgga catttataac cttcatccgg
      961 cacacgctaa acgtgtcgag gaaattatcg ctgaaaataa accgcccttt tctgttttcc
     1021 gcgacaaatt catcaccatg cctggcggga tggattattc ccgcgccatc gtggttgcgt
     1081 cagtgaaaga agcaccgatt ggtatcgagg
//
LOCUS       DAJTRB010000268         1106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTRB010000268 DAJTRB010000000
VERSION     DAJTRB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.278"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..452)
                     /locus_tag="OO330_004403"
     CDS             complement(<1..452)
                     /locus_tag="OO330_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5884983.1"
                     /translation="MLMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDA"
     gene            complement(403..1106)
                     /locus_tag="OO330_004404"
                     /pseudo
     CDS             complement(403..1106)
                     /locus_tag="OO330_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          243 a          306 c          281 g          276 t
ORIGIN      
        1 gcatcactcc acaccatatg tgtttgtgcc gcgacagatt ttgccagcat gaccaccggg
       61 gacaggcggg ccactgacga ggcgtcagcg ttccacgtgc ggccgttcca ttcaaacgtg
      121 aacggcttcg cctcctgttc tgtgcgccat gcttcaattt cctgacgtct ggcctctctg
      181 gccgcttcca gcatttctgg tgtcacagtg aatggggcta tctcacccca tttgccactt
      241 tgcagttcct gccagatttg ctgacccgtc ggtgcaacat catcagcggt ggctgtgtag
      301 ggaactgcct ggtccctgtc gtcaaaaaaa acgtcacagt ctactgcgcc actttcggta
      361 taacggggat taatgatttt tttaatttcc acagtgcatt cctcacgatg tgcgaataaa
      421 aagcccgggc attgcgccag agacatgagc atccggcacc ccggacaggg cgcaatatga
      481 ccctggtaat gaatgctctg aacatcccgt aatgaaaaat tgtggggatg ctatatacgt
      541 tccggtggga gtactgggca ctgaaatccc caccggtccc agtcgtgagc ctctgtatga
      601 ctgccccctg acaagtctga tgactttatc accgtcagct tctccctggt acgcagcaat
      661 aatcagcccg ccaatgtcag ggtctcccca tctgttgcgg acagagctcg ccacgattct
      721 gtaaataata tcttctgtgg ttatatttat tttcacccag tcagtctgga tatgggccag
      781 tagcggtagc gggtgtgata aatggggccg ttaatgccgt aaaaagtaag ggatttggct
      841 ctgtaccgcg gttctgttgt ctcagggcgt gcatcagtcc accggatgct gagcacccct
      901 tcaaaccgtg tgtcgggtat gatgatgccg taggggccag caacggaata ttcacctggc
      961 agcgcattcc ttacccaggc caggaaatca ctcttagtgt caaaacggat aacatcttca
     1021 ggcagaaaag cacacccaaa gccgaatgcg ccgggtatcg ccagacggcc ttttgtccgg
     1081 tcgtaaatgt cgctctgtgc ttccat
//
LOCUS       DAJTRB010000269         1088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTRB010000269 DAJTRB010000000
VERSION     DAJTRB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.279"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO330_004405"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO330_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5884984.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          347 a          158 c          194 g          389 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gggtaatgct gccaacttac tgatttagtg tatgatggtg attttaaggt
     1081 gcttgcgt
//
LOCUS       DAJTRB010000270         1072 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTRB010000270 DAJTRB010000000
VERSION     DAJTRB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.280"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..991
                     /locus_tag="OO330_004406"
                     /pseudo
     CDS             1..991
                     /locus_tag="OO330_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          301 a          263 c          254 g          254 t
ORIGIN      
        1 gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt tactgggttg cgctcccgct
       61 tacgacgcaa aattcccttt cacaatacca acccgaatgg cagtgctggg aacctgatgt
      121 cgaatgggtg cgtcagcccc cgcaagatgc gataaccgct cctgactttt tctgctttta
      181 ccagccaggc atgaccttcg aacaatttgt acgtgagttt gccgaatggt tttcacaaaa
      241 acgtccggcg gcgatgatga tcggcatccg tgcggatgag tcctacaacc gttttgtcgc
      301 catcgccagt ttaaataaac aacgttttgc cgacgataaa ccatggacta cggccgcacc
      361 aggcggtcat agctggtaca tttaccccat ttacgactgg aaagtggctg atatctggac
      421 ctgatatgca aatcatcaaa atctctgcaa cccactgtat aacctaatgt atcaggcagg
      481 cgttcctctg cgtcatatgc gaatttgcga accttttggc ccggagcaac gacaaggatt
      541 atggctctgt cacgttatcg aaccggatcg ctgggctgct atgtgcgtac gagtcagcgg
      601 ggtaaaaagt ggcggcattt acgccggaca tgacaatcat ttctatgggc atcggaaaat
      661 cctcaaacca gaacatttag actggcaaga atatgcatta ttgctgctca atagcatgcc
      721 ggaaaaaaca gctgagcatt accgcaataa aatcgccatt tatttgcact ggtatcagaa
      781 aaaaggcatc gaggttccac aaacccagca aggggacatt ggcgcgaaag atatcccctc
      841 ctggcggcgg atatgcaaag ttttactcaa taacgattac tggtgtcggg cattatcatt
      901 tagtcctacg aaagcgaaga actatcagcg ttataacgaa cggataaaag gaaaacgtca
      961 ggaatggggg atactatgca acaacgatta acgcaggatt taacgcaatc tctcgccagc
     1021 ttgccggaag atgatcgcat caaagcgatt aatgaaatcc gcatggcgat cc
//
LOCUS       DAJTRB010000271         1065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTRB010000271 DAJTRB010000000
VERSION     DAJTRB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.281"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(569..748)
                     /locus_tag="OO330_004407"
                     /pseudo
     CDS             complement(569..748)
                     /locus_tag="OO330_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            842..>1065
                     /locus_tag="OO330_004408"
     CDS             842..>1065
                     /locus_tag="OO330_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5884985.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          310 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg atgctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agtggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc ccctgaatct ccagacaacc
      781 aatatcactt atttaagtga tagtcttaat actagttttt agactagtca ttggagagca
      841 gatgattgat gtcttaggac cggagaaacg cagacggcgt actacacagg aaaagatcgc
      901 tattgttcag cagagttttg aaccgggaat gacggtctcc cttgttgccc ggcaacacgg
      961 tgtggcagcc agccagttat ttctctggcg caagcaatac caggagggaa gtcttactgc
     1021 tgtggccgca ggagaacagg tcgttcctgc ctctgaactt gctgc
//
LOCUS       DAJTRB010000272         1045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTRB010000272 DAJTRB010000000
VERSION     DAJTRB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.282"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            45..290
                     /gene="cusF"
                     /locus_tag="OO330_004409"
     CDS             45..290
                     /gene="cusF"
                     /locus_tag="OO330_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux system protein CusF"
                     /protein_id="HCR5884986.1"
                     /translation="MSEAQPQVISATGVVKGVDLESKKITIHHDPIAAVNWPEMTMRF
                     TITPQTKMSEIKTGDKVAFNFVQQGNLSLLQDIKVSQ"
     gene            306..>1045
                     /locus_tag="OO330_004410"
     CDS             306..>1045
                     /locus_tag="OO330_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5884987.1"
                     /translation="MKKIALIIGSMIAGGIISASGFTWFDQAEPHAEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKEEGRLLICV"
BASE COUNT          276 a          255 c          280 g          234 t
ORIGIN      
        1 ttattggctt taatgcccag gctaacgaac atcatcatga aaccatgagc gaagcacaac
       61 cacaggtcat tagtgccacc ggtgtggtaa aaggtgttga tctggaaagc aaaaaaatca
      121 ccatccatca cgatccgatt gctgccgtca actggccgga gatgaccatg cgctttacca
      181 tcaccccgca gacgaaaatg agtgaaatta aaaccggcga caaagtggcg tttaattttg
      241 tccagcaggg caacctttct ttattacagg atattaaagt cagccagtaa cccaggttta
      301 atgagatgaa aaaaatcgcg cttattatcg gcagcatgat cgcgggcggt attatttcag
      361 cgtcaggttt tacctggttt gaccaggcgg aaccgcacgc agaaaaaatg ccgaccgcag
      421 aacgtaaaat cttattctgg tacgacccga tgtatcccaa tacgcggttc gataaaccag
      481 gtaaatcgcc gtttatggat atggatctgg tgccgaaata tgccgatgaa gagagttctg
      541 cgtctggtgt gcgcattgac ccgactcaga cgcagaatct gggggtgaaa acggctaccg
      601 tcacgcgcgg gccgctgact tttgcccaga gtttcccggc gaatgtcagt tacaacgagt
      661 atcagtatgc cattgtgcag gctcgcgctg ccgggtttat cgacaaggtg tatccgctta
      721 ccgtgggcga taaagtacaa aagggcacac cgcttctcga cctgaccatt cccgactggg
      781 tggaagcgca gagtgagtat ttactgttgc gcgaaaccgg cggtacggcg acccagactg
      841 aaggcattct tgagcggctg cgactggcgg gaatgccgga ggcggatatt cgccacctga
      901 tcgccacgca aaaaatccag actcgcttta cgctcaaagc gcccattgat ggcgtgatca
      961 ccgcgtttga tctgcgcgcg ggaatgaata tcgccaaaga taacgtggtc gcgaaagagg
     1021 aaggtaggtt gcttatctgt gtctg
//
LOCUS       DAJTRB010000273         1043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTRB010000273 DAJTRB010000000
VERSION     DAJTRB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.283"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..269
                     /locus_tag="OO330_004411"
     CDS             <1..269
                     /locus_tag="OO330_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5884988.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="OO330_004412"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="OO330_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5884989.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>1043
                     /locus_tag="OO330_004413"
     CDS             613..>1043
                     /locus_tag="OO330_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5884990.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPA"
BASE COUNT          280 a          278 c          285 g          200 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac ccgatcgcca gtaagcctga atccccggga
      961 cgaaaatctt cacgaaaacc gctgccggca gaacttcccc gggagacaca tcgccttctg
     1021 cctgctgaaa ccagttgccc ggc
//
LOCUS       DAJTRB010000274         1035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTRB010000274 DAJTRB010000000
VERSION     DAJTRB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.284"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            492..668
                     /locus_tag="OO330_004414"
     CDS             492..668
                     /locus_tag="OO330_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884991.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLMREYSD
                     DTAHDVSGLNTFIR"
BASE COUNT          263 a          267 c          238 g          267 t
ORIGIN      
        1 ttgttcgaaa ccggggcacc cggcagtttc tgtaatctgc gaagctggcg atcggtcaga
       61 ctcaaatgcc tggcaacttc agtctgagta gccactgttc acctcgccaa aaaatctccc
      121 ctcatgagtt ttaaacatgc atcttgcgaa caactttatg aaacgcggca tatatgtccg
      181 gtttgagtgt tcaatttttg cgcatgtccg gttcgcagaa agcgcatttc tatatttttc
      241 aaatggttaa ccttcagaga aaccggacat ggtttccgaa aaattttcat aaatagtgaa
      301 attctgcgcc gctcccgccc cgtggcaggc caccccaccg ggaggacccg tcagcctgac
      361 agccatgacg aacgtctgat acagcgccct gcatgaatga catcgggata atccagaaag
      421 gaatagcatc gtacccacaa gaatcagtgt aagtgtcctg tttcttccac ccccgcacag
      481 gactggcgag catgagggac aaacccgcga accataaacg cggtaaaaac ccggtgtgca
      541 tcgtttttga ttattcccgc acactcgcgc agaaggagtt ccccgtcggg ctacggtcat
      601 ggttaatgcg ggaatacagc gacgatacag cgcatgatgt gtcaggcctg aataccttta
      661 tccgttaaaa gggatatcag ttaagttatc ccgtgtaggg tataagccat tacaaagccc
      721 accagtagat gggctttgta atgaagagcc gttgtgaaag tagctctcag gttgtcattt
      781 tttccctttt ctgcctacct gcccactgtt ttgcaatgta caggcagtca tcgaaaactc
      841 ggccttttct gctggcttgt gaacaacggc ggtaatgctc caccgccatg tcagcgccag
      901 tcatagccgc gttctggtcg tacccaagct ttattaactc ggttgttaca tttttatgta
      961 tgaaatcaat cggagtcatg ctggtttacc atcagggaaa tcccccatgt cacatagctt
     1021 gaactgaatc agatc
//
LOCUS       DAJTRB010000275         1027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTRB010000275 DAJTRB010000000
VERSION     DAJTRB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.285"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            445..>1027
                     /locus_tag="OO330_004415"
     CDS             445..>1027
                     /locus_tag="OO330_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5884992.1"
                     /translation="MIKSTNIQAIGSRIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLE"
BASE COUNT          369 a          198 c          167 g          293 t
ORIGIN      
        1 gataaatgaa gccggaattg atgaaaaaca aagccaacgg tattctgatt tctgaagtta
       61 atttcagact cgtttaagct ccattttcaa aaaaatatat cagggtttgt tactaataac
      121 ttctgaaaac agaaaaaaaa tagagcagta attctatttc agagaggagt aactccatct
      181 atcctcgata cgtactgaac gttatcgtga tatattcatc atgctttctt caccattatt
      241 cgagtatagg gagagacgca atgaccttaa ataccttttt atggctgaat taccattctc
      301 aggaataaca gcagactcct tcccatagac ggcttagggg gaaagcaata cccggagaaa
      361 gggtacaaag caaactgttt taaaattaat aagctatatt ctatttaact agaattctta
      421 tcgttgataa gaattctagt tataatgatt aaatcaacca atatacaggc aatcggttct
      481 agaattatgc atcaaataaa caatgtatac tcgttaactc cattatcttt acctatggaa
      541 ctgactccat cttgtaatga attttattta aaaacctgga gcgaatggga aaagaacggt
      601 accccaggcg agcaacgcaa tatcgccttc aataggctga aaatatgttt acaaaatcaa
      661 gaggcagaat taaatttatc tgagctagat ttaaaaacat taccagattt accgcctcag
      721 ataacaacac tggaaataag aaaaaaccta ttaacacatc tccctgattt accaccaatg
      781 cttaaggtaa tacatgctca atttaatcaa ctggaaagct tacctgcctt acccgagacg
      841 ttagaagagc ttaatgcggg tgataacaag ataaaagaat taccatttct tcctgaaaat
      901 ctaactcatt tacgggttca taataaccga ttgcatattc tgccactatt gccaccggaa
      961 ctaaaattac tggtagtttc tggaaacaga ttagacagca ttcccccctt tccagataag
     1021 cttgaag
//
LOCUS       DAJTRB010000276         1004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTRB010000276 DAJTRB010000000
VERSION     DAJTRB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.286"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..534
                     /locus_tag="OO330_004416"
                     /pseudo
     CDS             1..534
                     /locus_tag="OO330_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          311 a          220 c          209 g          264 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ctattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatacaaa aacaaataat
      721 accgttgcaa taattccttt cagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 aaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca agaaaatatt
      841 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt
      901 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat
      961 ggaaaaacga atccgaataa agcaacaaaa gcattacaaa aaat
//
LOCUS       DAJTRB010000277          983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTRB010000277 DAJTRB010000000
VERSION     DAJTRB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.288"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO330_004417"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO330_004417"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5884993.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPFSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          260 a          220 c          221 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga aaaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJTRB010000278          977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTRB010000278 DAJTRB010000000
VERSION     DAJTRB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.289"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..200
                     /locus_tag="OO330_004418"
                     /pseudo
     CDS             1..200
                     /locus_tag="OO330_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..926
                     /locus_tag="OO330_004419"
                     /pseudo
     CDS             300..926
                     /locus_tag="OO330_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
BASE COUNT          305 a          228 c          219 g          225 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatcggaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aaggctgagg tgtactggca atagcggaca ctaccatttg
      961 ttcttttttt aagcagc
//
LOCUS       DAJTRB010000279          969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTRB010000279 DAJTRB010000000
VERSION     DAJTRB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.290"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(50..190)
                     /locus_tag="OO330_004420"
     CDS             complement(50..190)
                     /locus_tag="OO330_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884994.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..>969
                     /gene="queE"
                     /locus_tag="OO330_004421"
     CDS             329..>969
                     /gene="queE"
                     /locus_tag="OO330_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5884995.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLS"
BASE COUNT          275 a          198 c          232 g          264 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgat
//
LOCUS       DAJTRB010000280          967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTRB010000280 DAJTRB010000000
VERSION     DAJTRB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.291"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(3..485)
                     /locus_tag="OO330_004422"
     CDS             complement(3..485)
                     /locus_tag="OO330_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5884996.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO330_004423"
     CDS             complement(476..778)
                     /locus_tag="OO330_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5884997.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          294 a          236 c          195 g          242 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgc
//
LOCUS       DAJTRB010000281          932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTRB010000281 DAJTRB010000000
VERSION     DAJTRB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.292"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(55..243)
                     /locus_tag="OO330_004424"
     CDS             complement(55..243)
                     /locus_tag="OO330_004424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5884998.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          182 c          216 g          289 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgatcctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aa
//
LOCUS       DAJTRB010000282          900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTRB010000282 DAJTRB010000000
VERSION     DAJTRB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.293"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>900
                     /locus_tag="OO330_004425"
     CDS             <1..>900
                     /locus_tag="OO330_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5884999.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRAFSAFLDR
                     LSDTVSARNTSGFREQVAAWLEK"
BASE COUNT          276 a          218 c          142 g          264 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat tctccgcgtt ccttgaccgc ctttccgata
      841 ccgtctctgc acgcaatacc tccggattcc gtgaacaggt cgctgcatgg ctggaaaaac
//
LOCUS       DAJTRB010000283          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTRB010000283 DAJTRB010000000
VERSION     DAJTRB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.294"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..256)
                     /locus_tag="OO330_004426"
     CDS             complement(<1..256)
                     /locus_tag="OO330_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885000.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
     gene            281..520
                     /gene="relB"
                     /locus_tag="OO330_004427"
     CDS             281..520
                     /gene="relB"
                     /locus_tag="OO330_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5885001.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            520..807
                     /gene="relE"
                     /locus_tag="OO330_004428"
     CDS             520..807
                     /gene="relE"
                     /locus_tag="OO330_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5885002.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          240 a          175 c          219 g          229 t
ORIGIN      
        1 acatgctaaa gcaaaaaata gatgaataag ttgagttgtg catatgtagc ctgaccgtca
       61 caaagtatat ggtgtctgta ccagtaagat gatggccgga ctctttaaaa aagagctgac
      121 ctgcacaata caggatggac ttagcaatgg ctgctcctgg cacaaagcgg acagtgatca
      181 ccgttcttac gactactttc tgacttcctt cgtgacttgc cctaagcatg ttgtagtgcg
      241 atacttgtaa tgacatttgt aattacaaga ggtgtaagac atgggtagca ttaacctgcg
      301 tattgacgat gaacttaaag cgcgttctta cgccgcgctt gaaaaaatgg gtgtaactcc
      361 ttctgaagcg cttcgtctca tgctcgagta tatcgctgac aatgaacgct tgccgttcaa
      421 acagacactc ctgagtgatg aagatgctga acttgtggag atagtgaaag aacggcttcg
      481 taatcctaag ccagtacgtg tgacgctgga tgaactctga tggcgtattt tctggatttt
      541 gacgagcggg cactaaagga atggcgaaag ctgggctcga cggtacgtga acagttgaaa
      601 aagaagctgg ttgaagtact tgagtcaccc cggattgaag caaacaagct ccgtggtatg
      661 cctgattgtt acaagattaa gctccggtct tcaggctatc gccttgtata ccaggttata
      721 gacgagaaag ttgtcgtttt cgtgatttct gttgggaaaa gagaacgctc ggaagtatat
      781 agcgaggcgg tcaaacgcat tctctgaacc aaagcatgac atctctgttt cgcaccgaag
      841 gtgacacttc tgctttgcgt tga
//
LOCUS       DAJTRB010000284          861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTRB010000284 DAJTRB010000000
VERSION     DAJTRB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.295"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..101)
                     /gene="rrf"
                     /locus_tag="OO330_004429"
     rRNA            complement(<1..101)
                     /gene="rrf"
                     /locus_tag="OO330_004429"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(197..>861)
                     /locus_tag="OO330_004430"
     rRNA            complement(197..>861)
                     /locus_tag="OO330_004430"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          181 a          275 c          196 g          209 t
ORIGIN      
        1 actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc
       61 ggcatggggt caggtgggac caccgcgcta cggccgccag gctaattctg ttttatcaga
      121 ccgcttctgc gttctgattt aatctgtatc aggctgaaaa tcttctctca atccgccaaa
      181 acatcttcgg cgttgtaagg ttaagcctca cggttcatta gtaccggtta gctcaacgca
      241 tcgctgcgct tacacacccg gcctatcaac gtcgtcgtct tcaacgttcc ttcaggaccc
      301 ttaaagggtc agggagaact catctcgggg caagtttcgt gcttagatgc tttcagcact
      361 tatctcttcc gcatttagct accgggcagt gccattggca tgacaacccg aacaccagtg
      421 atgcgtccac tccggtcctc tcgtactagg agcagccccc ctcagttctc cagcgcccac
      481 ggcagatagg gaccgaactg tctcacgacg ttctaaaccc agctcgcgta ccactttaaa
      541 tggcgaacag ccataccctt gggacctact tcagccccag gatgtgatga gccgacatcg
      601 aggtgccaaa caccgccgtc gatatgaact cttgggcggt atcagcctgt tatccccgga
      661 gtacctttta tccgttgagc gatggccctt ccattcagaa ccaccggatc actatgacct
      721 gctttcgcac ctgctcgcgc cgtcacgctc gcagtcaagc tggcttatgc cattgcacta
      781 acctcctgat gtccgaccag gattagccaa ccttcgtgct cctccgttac tctttaggag
      841 gagaccgccc cagtcaaact a
//
LOCUS       DAJTRB010000285          841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTRB010000285 DAJTRB010000000
VERSION     DAJTRB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.296"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..525
                     /gene="istA"
                     /locus_tag="OO330_004431"
                     /pseudo
     CDS             1..525
                     /gene="istA"
                     /locus_tag="OO330_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
     gene            complement(551..>841)
                     /locus_tag="OO330_004432"
     CDS             complement(551..>841)
                     /locus_tag="OO330_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HCR5885003.1"
                     /translation="YLSLRASKSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLK
                     YQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          196 a          212 c          210 g          223 t
ORIGIN      
        1 gctgttgctt cactcctgga tgaataccgg gattatattc gtcaacgcat cgccgatgct
       61 catccttaca aaatcccggc aacggtaatc gctcgcgaga tcagagacca gggatatcgt
      121 ggcggaatga ccattctcag ggcattcatt cgttctctct cggttcctca ggagcaggag
      181 cctgccgttc ggttcgaaac tgaacccgga cgacagatgc aggttgactg gggcactatg
      241 cgtaatggtc gctcaccgct tcacgtgttc gttgctgttc ccggatacag ccgaatgttg
      301 tacatcgaat tcactgacaa tatgcgttat gacacgctgg agacctgcca tcgtaatgcg
      361 ttccgcttct ttggtggtgt gccgcgcgaa gtgttgtatg acaatatgaa aactgtggtt
      421 ctgcaacgtg acgcatatca gaccggtcag caccggttcc atccttcgct gtggcagttc
      481 ggcaaggaga tgggcttctc tccccgactg tgtcgcccct tcaggctgag ggatccccac
      541 aaaattactg ctaataaacc agcaccatat ttcggtaggt tctggacaga tggtttagaa
      601 ctgtgctcag tactgttcgt cttaaaataa gtggcgagtg tcgaagaaca ttcttcgcta
      661 acgtcagata tgagataacc cgccgggatt taatgctgtt agcctgatat ttcaggtgta
      721 accctttatt ttcagcgtga taaccgagta accacattac gatagtgctc aacgttgcca
      781 gcagactcag aaccagaatt cttcctgttg aacgactttt gctggcccga agactgaggt
      841 a
//
LOCUS       DAJTRB010000286          828 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTRB010000286 DAJTRB010000000
VERSION     DAJTRB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.297"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            211..684
                     /locus_tag="OO330_004433"
     CDS             211..684
                     /locus_tag="OO330_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5885004.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          257 a          172 c          183 g          216 t
ORIGIN      
        1 gagcaggtac cggcatccac gttacagacg gtcccggcaa aagtgcctga cctgacccct
       61 attagcggtt accagcgccc caccagaccc ggtgacatca ccccccggat catggacgac
      121 tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata
      181 aagtaacggg ggggagatga accctatgca atgtcctggt taatatctca gtgtgctcat
      241 cagtgcactg ataataaaaa gacggagaca gatgccattt acgataaagt tcggagttct
      301 tatttgcttt catgtattct gaaaaaaaac aaaaatgttg ggcttattct tcatgcacca
      361 tcatttgttt ctgtgtctga aaaaatcgcc agaattgtga tggcaaatta ctcccgtaac
      421 tggagtaatt ccgagttggc cagtgcggtt ttaatgagtg aatcttcttt aaaaagaaga
      481 atgtacaaag aggtcggttc tataagtaca tttgtacata aaataaagct tacagaagct
      541 atcaggaaat tacgaaggac aaatactccc atttctgtca tatcatctga gttaggttac
      601 agttcaccat cgtatttttc taaagtcttc tttaaatatt taaagacata ccctcaaaac
      661 ataaggaaga aaaatggtcg gtaatgtcac caattaggag gacttgcaga gccgcttttt
      721 tacgatactg agatcaaaca gacataatct atgcggagat ttacagcagg gcatggttca
      781 caaatctgac agcgatgaat tatcggcact gcgggcagaa aatgctcg
//
LOCUS       DAJTRB010000287          817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTRB010000287 DAJTRB010000000
VERSION     DAJTRB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.298"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            29..>817
                     /gene="ltrA"
                     /locus_tag="OO330_004434"
     CDS             29..>817
                     /gene="ltrA"
                     /locus_tag="OO330_004434"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5885005.1"
                     /translation="MKDRNGSGAKGLPHCADGAAATTGDNADGRTAVKSAKPFPVSKR
                     QVWEAYKRVKANRGAAGIDGQTLAGFDENVTDNLYKLWNRMASGSYMPQAVRRVDIPK
                     ADGGVRPLGIPAVSDRIAQMVVKQILEPVLEPLFHADSYGYRPGKSAHQAIAQARKRC
                     WKFDWVVEVDIKGFFDDIDHDLLLKTVQHHTQARWVVMYIERRLKAPVQMPDGAMLAR
                     GRGTPQGGVISPLLSNLLLHYAFDMWMQRQFPGVPFERYADDVVC"
BASE COUNT          194 a          195 c          256 g          172 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgctcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgc
//
LOCUS       DAJTRB010000288          793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTRB010000288 DAJTRB010000000
VERSION     DAJTRB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.299"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(1..249)
                     /locus_tag="OO330_004435"
                     /pseudo
     CDS             complement(1..249)
                     /locus_tag="OO330_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(246..773)
                     /locus_tag="OO330_004436"
                     /pseudo
     CDS             complement(246..773)
                     /locus_tag="OO330_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT          233 a          194 c          147 g          219 t
ORIGIN      
        1 tttttcttaa accacgaaaa atcgatattc ttcgtgacgc atttataagc ccagtacgcc
       61 ttgagatcaa aaataaaatt atacagcacc agggccagaa tcccactaac tacaccaata
      121 tcctctgatg gaaaagtaaa ccctgcattt ttccaataaa atgcctatca gaataagtcc
      181 aatagcataa atgtatatca gatttcgttt atcctgaaag cgaataaaag ttaaataatc
      241 aggaatcatt tgaatacctt tgcatcacta aatgctttac gacaatgcat aactcgttgc
      301 tgatccacgc cagcaaaatc atttaacata ttccccaaat agggcttatt acttatttca
      361 aggaaagcta ccgggttggc atattcagaa tatgtatcga gagattcagt ccatttcacc
      421 gtatgagtta agtgtaatga gagattttct ttgataattg acggagcgac ctcgcttttt
      481 gctgttacgt tcatcagaac ctgatgctct ggcgaagcga tatccagtcc ggcaagataa
      541 tcgcgcattg cctgaacgcc gtcttccatc aaacgcgtgt gccaggcacc gctcacaccg
      601 agtttaaccg gttcgtatcc agcggccatc agcagcgtgg taaattcatt caacgcagcc
      661 tgcgttcccc caataacctg ctggcgcggc gtgttatcac agctaatatc cagcgcaatg
      721 cctgattccg taatcatcgt ctgcagttgt tcgcggttga tgcctttaac cgcctgatct
      781 tacccagcaa tag
//
LOCUS       DAJTRB010000289          791 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTRB010000289 DAJTRB010000000
VERSION     DAJTRB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.300"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            115..>791
                     /locus_tag="OO330_004437"
     CDS             115..>791
                     /locus_tag="OO330_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5885006.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATML"
BASE COUNT          178 a          164 c          196 g          253 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc t
//
LOCUS       DAJTRB010000290          782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTRB010000290 DAJTRB010000000
VERSION     DAJTRB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.301"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OO330_004438"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OO330_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5885007.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFISNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          282 a          105 c          158 g          237 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat gtcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattag taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa aaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtggtg aacgccccgg gaatcctgga
      781 ga
//
LOCUS       DAJTRB010000291          778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTRB010000291 DAJTRB010000000
VERSION     DAJTRB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.302"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            105..>778
                     /locus_tag="OO330_004439"
     CDS             105..>778
                     /locus_tag="OO330_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5885008.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVM"
BASE COUNT          191 a          186 c          239 g          162 t
ORIGIN      
        1 tgttcgcgcc gaaaggtgtt cggcctgaga cgttgctccg acaggttaac accgtctcgt
       61 tgccggacag tgcgatcccg ctaggcttca gggggtaaca gaccatgaaa aatattgcga
      121 caggaggcgt tctggagcgt atccgcagac tgaccccacc acatgtaacc gccccattca
      181 gaacggttgc ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt tgcgaggaga
      241 tcaaccgcct gaatcgtcag ttgcgggtgg aaaaaatcct gaaccgctcc ggcatccagc
      301 cgttgcaccg gaagtgttcg tttgcgaatt accgggcgca gaacgacggt cagcgacatg
      361 cactgagtca ggcgaaatcc atagctgacg aactgatgac aggctgtacg aattttgtgt
      421 tcagcggtaa gcccggcacc ggaaaaaatc atcttgcagc cgcgattggc aaccggctga
      481 tggcgaaggg gcgtagcgtg attatcgtca ccgtgtccga tgtcatgagc gtgttgcatg
      541 agagctacga caacggcaaa tccggtgaaa aatttttaca ggagctttgt ggtgttgacc
      601 tgctggtcct ggatgaaatt ggcatgcagc gggatacgaa aaacgagcag gtggtactga
      661 accagattgt tgatcgccgg acggcatcgt tacgcggtgt ggggatgctg acaaatatta
      721 accatgcagc gatgaataca cttctcggcg agcgggtgat ggatcgcatg gtcatgaa
//
LOCUS       DAJTRB010000292          769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTRB010000292 DAJTRB010000000
VERSION     DAJTRB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.303"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..229)
                     /gene="mraZ"
                     /locus_tag="OO330_004440"
     CDS             complement(<1..229)
                     /gene="mraZ"
                     /locus_tag="OO330_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5885009.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRL"
BASE COUNT          218 a          172 c          173 g          206 t
ORIGIN      
        1 gtaggcgctg cacacggcgc tcaaccgggt tcatgctcaa cagacgcgat aatttttgct
       61 cgataatttc ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg
      121 tgcaaaccat ttgaccggca gcgttctcaa gcagctgttc ccgataacgg gtaggcactg
      181 ataagcgccc tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac
      241 cccttattcc cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa
      301 agcttgtcaa gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata
      361 tcagcgttgt ttattttacg tccaacgtgc gaagcaaatt aacgttatga atagctgtaa
      421 gaaaaaatcc ctcatcgtca tcactgtttt aaaatgactc actattttcc acagcaaata
      481 taaagtgtca gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc
      541 cttgttacgt ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgt
      601 ttgttaaatc ggcaattcat cgccgcatgt aacaaaataa tgcaaaaacg cgctgtcgat
      661 atcacttaat tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaaccat
      721 ttatcaataa tagtcaggaa tagtcttatt tactttaagg atattgatg
//
LOCUS       DAJTRB010000293          750 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTRB010000293 DAJTRB010000000
VERSION     DAJTRB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.304"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>750
                     /locus_tag="OO330_004441"
     CDS             <1..>750
                     /locus_tag="OO330_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5885010.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKAL"
BASE COUNT          208 a          153 c          180 g          209 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      361 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      421 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      481 cggtttctca tttgctggtg cttatgcaaa ctctgaccgt actgattggc agtctggtga
      541 tggtaaaggc gaaagagccg aagtttgggc gttgtcaaca aaatatgacg ctaacaatgt
      601 ttatgctgct gtgatgtacg gtgaatctca caacatgaat tccgacgacg gtgatgttgt
      661 gaacaaaaca caaaacttcg aagctgttct gcaatatcag ttcgactttg gtctgcgtcc
      721 atccatcggt tactcctatt ctaaagcact
//
LOCUS       DAJTRB010000294          746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTRB010000294 DAJTRB010000000
VERSION     DAJTRB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.305"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..109
                     /locus_tag="OO330_004442"
     CDS             <1..109
                     /locus_tag="OO330_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5885011.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
     gene            447..746
                     /locus_tag="OO330_004443"
                     /pseudo
     CDS             447..746
                     /locus_tag="OO330_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          228 a          157 c          176 g          185 t
ORIGIN      
        1 aaatagtggg gacactatgc ttgacaatgc aatgaaaagc aaagattcaa aaatgattga
       61 ttttttatta aaaaatggag caatattagg caaacgattt gagatatgac ctaaaagctc
      121 ggccaaataa taatgtcgtc gattatcgaa gtcatcatga gtgattctgt ccccacagtg
      181 cagcatcagc atgacagtga attgtctggc gtggtccatt taatgagatg gagatggtcc
      241 ctctttccta aatagctgtg cctaataccg ctacactttt gccagcccat gtttgcctcc
      301 ggggaatggg ctgacggttc cttcaaaatc cattgcacgc agttatgtcg gagcgggaac
      361 ttgactcctg tctttacggg agtgaggtag cctgagttta acggacactc cttcctgaaa
      421 tagaatggca tcagaaggag ccaataatga gcagaaaaac ccaacgttac tctacggagt
      481 ttaaagccga agccgtcaaa acggttcctg aaaatcagct ttcgatcagt gaaggcgctt
      541 cccgattatc tgttcctgaa ggcactttag gacaatgggt taccgccgca agaaaagggc
      601 tcaatacacc aggctcccgc acagtggctg agctggaatc tgaagttatg caactgcgta
      661 aggctttaaa tgaagcacga cttgagcgag atatattaaa aaaagcaaca gcgtattttg
      721 cacaggagtc gctgaaaaat acgcgt
//
LOCUS       DAJTRB010000295          707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTRB010000295 DAJTRB010000000
VERSION     DAJTRB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.306"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>707
                     /locus_tag="OO330_004444"
     CDS             <1..>707
                     /locus_tag="OO330_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR5885012.1"
                     /translation="LHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTA
                     QLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQM
                     ELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHR
                     HWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVC
                     HLLTSMTDAMRFPGGEMA"
BASE COUNT          178 a          205 c          202 g          122 t
ORIGIN      
        1 cttcaccaga ttgtgaatcg cctggacatc atgctgccgg gcaatcgccc cttcgttgcc
       61 cccagtgccg ttattcaggc ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc
      121 acgaaaacag cgcagctctg gcataacgcc acgccgcatc cgcactggtg cggcctgacc
      181 ctgctggcca tcgatggtgt gttctggcgc acaccggata caccagagaa cgatgcagcc
      241 ttcccccgcc agacacatgc cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc
      301 cagatggaac tgaccagcca tctgctgacg gctgcagcct tcggcacgat gaagaacagc
      361 gaaaatgagc ttgctgagca acttatagaa caaaccggcg ataacactct gacgttaatg
      421 gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg agaacaccgc
      481 cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa actgggtaaa
      541 ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg
      601 ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg
      661 ctgacgtcga tgacggacgc catgcgcttc cccggaggag aaatggc
//
LOCUS       DAJTRB010000296          699 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTRB010000296 DAJTRB010000000
VERSION     DAJTRB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.307"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            15..560
                     /locus_tag="OO330_004445"
     CDS             15..560
                     /locus_tag="OO330_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5885013.1"
                     /translation="MLHLKNITTGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            584..>699
                     /locus_tag="OO330_004446"
     CDS             584..>699
                     /locus_tag="OO330_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5885014.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVA"
BASE COUNT          205 a          152 c          178 g          164 t
ORIGIN      
        1 gcgagtattt aacaatgttg catttaaaaa atattactac gggtaatccg aaaacagcag
       61 aacaatatca gatgacaaaa cgatattcgg tcacctggct tttttcagaa gacggaaaaa
      121 actggtatga agagctgaag aatttcgcca gcgacacaat aaaaatagct tacaccggag
      181 atggtcgcgt ggtgtgggtc ggtaaggatg tgacaggcat cgagccacgc aatgccagcg
      241 ttatcgaagt tccggatatt accgctaacc gacggattac cgcgccgggt tactggtttt
      301 accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc
      361 ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggttaa
      421 tcagcgacga agataaagaa aagctgaaag cctgtcgtat ttacgcgaaa tcgctgcagg
      481 cgatggattt cagcaccatc actgataaat cctcatacaa cgccattgaa tggcccgcct
      541 ctccggaagg ttcttcctga tttaatttat cgcgagaaaa acaatgtctg tagtgatatc
      601 aggtgcgctg actgatggcg caggtatccc catgtccgga taccatatta ttctgaaatc
      661 ccgggtaaac accccggaag tggtgatgaa cactgttgc
//
LOCUS       DAJTRB010000297          692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTRB010000297 DAJTRB010000000
VERSION     DAJTRB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.308"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            376..519
                     /locus_tag="OO330_004447"
                     /pseudo
     CDS             376..519
                     /locus_tag="OO330_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          201 a          166 c          125 g          200 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcgaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacataaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaaa taaatttttt gccaaaatat aacataagga
      661 agtaatatgc ttacacaaac tatattccct tg
//
LOCUS       DAJTRB010000298          669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTRB010000298 DAJTRB010000000
VERSION     DAJTRB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.309"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>669
                     /locus_tag="OO330_004448"
     CDS             <1..>669
                     /locus_tag="OO330_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5885015.1"
                     /translation="PLKKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHG
                     ELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEM
                     TMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKR
                     AGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIE
                     LLNR"
BASE COUNT          221 a          148 c          146 g          154 t
ORIGIN      
        1 caccattaaa aaagtccaag atagaaccag atcccctcac ccgtgacgaa tttattcgtt
       61 ttattgaggc ttgccgtcat caacaaacaa aaaacctgtg gattatcgct gtatacacgg
      121 gtattcgtca cggggagctg gtatcactgg cctgggaaga tatagacctt aaagcgagga
      181 ctataaccat ccgccggaat tatacaaaac taggcgaatt cactccacca aaaactgatg
      241 ctggtaccgg aagaacgatt catctagttc aaccagctat tgatgctctt aaaagccaag
      301 cggaaatgac catgcttgga aaacagcatt ctgtagaggt aaagcagagg gaatatggga
      361 gaagtactgt gcataaatgc acttttgttt ttagtcctca ggtaataaaa cagcggcagt
      421 tttccggacc gcactataag gttgactcca tcagggagtc atggacaagt atcttaaaac
      481 gcgcaggtct gagacacaga aaatcgtatc aatccaggca tacctatgca tgctggtcac
      541 ttgccgcagg agctaatcct agttttatcg caagccagat gggccacaca aacgcacaaa
      601 tggtattcaa tgtttacgga gcatggatga aagacaacaa tcacgaacag atagaacttc
      661 ttaacagaa
//
LOCUS       DAJTRB010000299          663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTRB010000299 DAJTRB010000000
VERSION     DAJTRB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.310"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(71..>663)
                     /locus_tag="OO330_004449"
     CDS             complement(71..>663)
                     /locus_tag="OO330_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5885016.1"
                     /translation="LKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTE
                     FAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQ
                     GWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELK
                     DAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
BASE COUNT          177 a          151 c          130 g          205 t
ORIGIN      
        1 ggactaccat ccaaattgtc tggtttggag tggcagcccc agggtgtact gaccccaaaa
       61 agttggacag ttatacacga ggcatatagg tctgattccg atattcaatt ggagtcagac
      121 cttttaattt caggctaatt cttctgctgt tatagtattc aatatattcc gtaacagcat
      181 ccttcagttc gcttatatta ctgaactcat caagataaaa acactccgac tttaaggttc
      241 caaagaaaca ctccaccaca gcattatcca gacaattgcc ttttctggac atgctttgtt
      301 taataccatg ttctttaagg atattttgat atcttctcat acgatactgc catccctggt
      361 cagagtgcag aacaggatgc tcgtgaggat taagcttttt gaatgcctga tcgagcatat
      421 tctcaaccat gttcatcact ggtctttccg aaaggctgta agaaataact tcgttgttga
      481 agagatctat tactggagac aaatacagct tgcgcccatt gactgcaaat tcagtaacat
      541 cggtaaccca cttctcgttt ggccgcgtag ccttgaaatc tctttggaga acattagggg
      601 cggtttgccc tacctctcct ctgtaagagc ggtatcgctt gaccttaatc gctgctttaa
      661 gtg
//
LOCUS       DAJTRB010000300          658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTRB010000300 DAJTRB010000000
VERSION     DAJTRB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.311"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(151..657)
                     /locus_tag="OO330_004450"
     CDS             complement(151..657)
                     /locus_tag="OO330_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5885017.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          173 a          161 c          145 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaatc
//
LOCUS       DAJTRB010000301          641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTRB010000301 DAJTRB010000000
VERSION     DAJTRB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.312"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(16..>641)
                     /locus_tag="OO330_004451"
     rRNA            complement(16..>641)
                     /locus_tag="OO330_004451"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          138 a          191 c          152 g          160 t
ORIGIN      
        1 aacgcttctt taaggtaagg aggtgatcca accgcaggtt cccctacggt taccttgtta
       61 cgacttcacc ccagtcatga atcacaaagt ggtaagcgcc ctcccgaagg ttaagctacc
      121 tacttctttt gcaacccact cccatggtgt gacgggcggt gtgtacaagg cccgggaacg
      181 tattcaccgt ggcattctga tccacgatta ctagcgattc cgacttcatg gagtcgagtt
      241 gcagactcca atccggacta cgacgcactt tatgaggtcc gcttgctctc gcgaggtcgc
      301 ttctctttgt atgcgccatt gtagcacgtg tgtagccctg gtcgtaaggg ccatgatgac
      361 ttgacgtcat ccccaccttc ctccagttta tcactggcag tctcctttga gttcccggcc
      421 ggaccgctgg caacaaagga taagggttgc gctcgttgcg ggacttaacc caacatttca
      481 caacacgagc tgacgacagc catgcagcac ctgtctcacg gttcccgaag gcacattctc
      541 atctctgaaa acttccgtgg atgtcaagac caggtaaggt tcttcgcgtt gcatcgaatt
      601 aaaccacatg ctccaccgct tgtgcgggcc cccgtcaatt c
//
LOCUS       DAJTRB010000302          617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTRB010000302 DAJTRB010000000
VERSION     DAJTRB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.313"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..236
                     /locus_tag="OO330_004452"
     CDS             <1..236
                     /locus_tag="OO330_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5885018.1"
                     /translation="QKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIK
                     DGGRDCRIRSNNCYGQVIRRDQESALACWGIDQ"
     gene            233..617
                     /locus_tag="OO330_004453"
                     /pseudo
     CDS             233..617
                     /locus_tag="OO330_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
BASE COUNT          150 a          149 c          173 g          145 t
ORIGIN      
        1 cacagaaagc gggtatagcg tcattttgtc cctataacat tggcccaggt aagtgtttcc
       61 cgtcgacgtt ttataagcgg ctgaatgccg gtgatcgtaa gggcgcatgc gaggcgattc
      121 gctggtggat aaaagatggt gggcgcgatt gccgcatacg ttcaaataac tgctatggac
      181 aggttattcg tcgtgaccag gaaagcgcat tagcctgttg ggggatagat cagtgagctg
      241 agtcgccgcg attatttatg ctctggttat ctgcatcatc gtctgcctgt cgtgggcggt
      301 caatcattac cgtgataacg ccatcgccta caaagaacag cgtgataaaa aagtcagtga
      361 gctgaagctg gcgaccgcca ccattactga catgcagcaa cgccagcgtg ctgctgatgt
      421 actcgatgct aaatacacga aggagttggc tgatgcgaaa gctgaaaatg atgctcttcg
      481 gcgcaagctt gataatggtg gccgggtgct cgtcaaagga aaatgctctg tgccatcctc
      541 agccgaaacc tccagcgcct ccggcatggg caatgatgcc accgtcgaac tctctccagt
      601 tgctggacga aacgttc
//
LOCUS       DAJTRB010000303          613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTRB010000303 DAJTRB010000000
VERSION     DAJTRB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.314"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            41..>613
                     /locus_tag="OO330_004454"
     CDS             41..>613
                     /locus_tag="OO330_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5885019.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSGWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYE"
BASE COUNT          180 a          129 c          161 g          143 t
ORIGIN      
        1 cccaccacca ggttgatatt gatttggagg actaaaatct atggcgcttt ttcaacgagc
       61 aataaacaca caggcctatc taaaagcagg aattatgggg tttgctggcg acggcaaaac
      121 ctacacagca agcgaactgg ctattggtct ggtaatgctt atgcggcaac ggggaattga
      181 agctggtaac cgcccggtaa tgtttctgga cacagaaaca ggctctgact gggttaaacc
      241 ccgctttgat gcggaaaata tcgaattatt tacggcaaaa acccgctcgt ttgttgatct
      301 gcttgaagcc attaatgaag cggaaagcag cggttccgta atgatcatcg actccatcag
      361 tcacttctgg accggattat gcgacgagta tgccagacgc cgaaaccgta aacgaggact
      421 ggaattttca ggctgggcgt ggctcaaaca ggagtggcga cgctttaccg atcgatttgt
      481 taacagtcag gcgcacatta ttatgtgcgg gcgtgcagga tatgagtatg actttttcga
      541 aggtgatgat ggcaaacgcc agttggcaaa aaccggtata aaaatgaaag cagaaacaga
      601 aaccggttat gaa
//
LOCUS       DAJTRB010000304          568 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTRB010000304 DAJTRB010000000
VERSION     DAJTRB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.315"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>568
                     /locus_tag="OO330_004455"
     CDS             <1..>568
                     /locus_tag="OO330_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5885020.1"
                     /translation="DFVFSKDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESL
                     GIQGSEELFRNMIGYSRLMGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGM
                     VQVFAKATGKTEQELFDAMKKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQL
                     GNLKNSWNTTLDSIFRGSENGLQDFTRSL"
BASE COUNT          183 a           97 c          137 g          151 t
ORIGIN      
        1 tgatttcgta ttttctaaag atgcaaatca agtaaaagat ttcacaaagt cattagctca
       61 aacaacgggt ttagatatcg ctgaactgca atcacagttt gctggtttcg gtgcttcagc
      121 tagagagtct ctaggcattc aaggttctga agaacttttt agaaatatga ttggctattc
      181 tcgtctgatg gggcgaagtg aagaagagat taagcgagca ctgacagcat tatcacagat
      241 ggctggtaag ggtcagatta tggctgaaga gcttaagggg caactagctg aagctgttcc
      301 tggtatggtt caggtattcg ctaaagcaac cggaaagact gaacaggaat tatttgatgc
      361 tatgaagaag ggggctttaa aatctgccga tactcttcag aaggtcactc aggaactgaa
      421 taaacaaatc accgctaaag gtggctggaa agctatttca gaaagtacac aggcacaact
      481 agggaacttg aaaaactctt ggaataccac gcttgatagt atcttcagag gttctgaaaa
      541 tggtctgcaa gattttacaa gaagctta
//
LOCUS       DAJTRB010000305          566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTRB010000305 DAJTRB010000000
VERSION     DAJTRB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.316"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            8..208
                     /gene="glgS"
                     /locus_tag="OO330_004456"
     CDS             8..208
                     /gene="glgS"
                     /locus_tag="OO330_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR5885021.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(251..>566)
                     /locus_tag="OO330_004457"
     CDS             complement(251..>566)
                     /locus_tag="OO330_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5885022.1"
                     /translation="FSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLK
                     IFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKIT
                     YN"
BASE COUNT          155 a          115 c          131 g          165 t
ORIGIN      
        1 tgtgcttatg gatcatagtc ttaattcttt gaataatttc gatttcctgg cgcgtagttt
       61 tgccagaatg cacgcagaag gtcgcccggt cgatattctg gctgttactg gtaacatgga
      121 tgaagaacat agaacctggt tttgcgcacg ttatgcctgg tattgccaac agatgatgca
      181 ggcaagagag ctggagttag agcactgata taacgggcct gatggcccgt tttagtgttt
      241 gtattagatg ctagttatag gtgattttga ggattacgtc tttactaaac ggtccctcaa
      301 caacagtctc tgccgggttc gcactaagtt ctgccctgaa tggtgcggtg accgaaggtg
      361 cactagacga tgaaaaagaa gcaaattgtg agaagcggtt aaattccgtt gctgttccat
      421 ctttatgaaa tatcttcaat agcagtccat tacccatatc cagatggcta taatcactca
      481 aaatatccgt agtgaaaaag ctccctaaaa tattgaattg ctcagtacag gccgcaccag
      541 gatcttttgt catgactaca ctaaac
//
LOCUS       DAJTRB010000306          563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTRB010000306 DAJTRB010000000
VERSION     DAJTRB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.317"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..313
                     /locus_tag="OO330_004458"
     CDS             <1..313
                     /locus_tag="OO330_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5885023.1"
                     /translation="KPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQ
                     IKEYLDTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRP
                     Q"
     gene            complement(314..>563)
                     /locus_tag="OO330_004459"
     CDS             complement(314..>563)
                     /locus_tag="OO330_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885024.1"
                     /translation="VVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKM
                     EAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
BASE COUNT          151 a          136 c          136 g          140 t
ORIGIN      
        1 caaacctttc cactggcaat ttgcaaagga gctggaaatt gctgtcgcag gaaatatcat
       61 tctggatgat gttgatgctg aactggaggc cgttttgatg ggtgctggta tcgggtatct
      121 gctctatgaa cagatcaagg agtaccttga tactggtcgt ttagaatgtg tactggaaga
      181 ctggatcaca gagcgtccag gcttccaagt ctattaccca aaccgccagt atatgtcctg
      241 cggcttgcgc gcatttctcg attatgtaaa aacgggtcag atctgccaga gtcagcgtca
      301 ccgaccacaa taattatttg ctattcgctt tacgcgccag cagcgggaat atcagtccta
      361 atcctaccag cacaaacggc gttgcaacat tcagcgccaa ctggaaggtc cactctgcag
      421 tgaaggcttc cattttcggg aagatccccg tcaaacaggc acaggcggta aaggcaaaac
      481 accaaatccc gacggtcatt gccagaggct tattacgaat aaagacatat tctggtttat
      541 atttctgcgc caggcgaacg act
//
LOCUS       DAJTRB010000307          543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTRB010000307 DAJTRB010000000
VERSION     DAJTRB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.318"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            101..>543
                     /locus_tag="OO330_004460"
     CDS             101..>543
                     /locus_tag="OO330_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885025.1"
                     /translation="MAGVLEGRLSGGTAAEFCNSAVVALQAAGLDVCREYPVPERGDG
                     CGGRIDIVVTDRNGVRCGIELDRNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGIL
                     VIGGAVRQKKFDPLSVDLPDWLPETLWHEWVQFRQALRKPIRTELG"
BASE COUNT          128 a          117 c          169 g          129 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 ggg
//
LOCUS       DAJTRB010000308          543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTRB010000308 DAJTRB010000000
VERSION     DAJTRB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.319"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            3..>543
                     /locus_tag="OO330_004461"
     CDS             3..>543
                     /locus_tag="OO330_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5885026.1"
                     /translation="MHQINNIHSLTLFSLHVSLSPSCNEYYLKVWSEWEKNGIPGEQR
                     NIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNLLTYLPDFPPMLKVI
                     HAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHNNRLHILPLLPPELK
                     LLIVSGNRLDSIPPFPDKLE"
BASE COUNT          189 a          112 c           89 g          153 t
ORIGIN      
        1 ttatgcatca aataaacaac atacactcgt taactctatt ttctttgcat gtgagtttga
       61 gtccatcttg taatgaatat tatttaaaag tctggagtga atgggaaaag aacggtatcc
      121 caggcgagca acgcaatatc gccttcaata ggctaaaaat atgtttgcaa aatcaagggg
      181 cggaattaaa tctatctgag ttagatttaa aaacattacc tgatttaccg cctcagataa
      241 caacactgga aataagaaaa aacctattaa catatctccc tgatttccca ccaatgctta
      301 aggtaataca tgctcaattt aatcaactgg aaagcttacc tgccttaccc gagacgttag
      361 aagagcttaa tgtgggtgat aacaagataa aagaattacc atatcttcct gaaactctaa
      421 ctcatttacg gatccataat aaccgattgc atattctgcc actattgcca ccggagctaa
      481 aattactgat agtttctgga aacagattag acagcatccc cccctttcca gataagcttg
      541 aag
//
LOCUS       DAJTRB010000309          542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTRB010000309 DAJTRB010000000
VERSION     DAJTRB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.320"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..272
                     /locus_tag="OO330_004462"
     CDS             <1..272
                     /locus_tag="OO330_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5885027.1"
                     /translation="KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSR
                     YSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(380..>542)
                     /locus_tag="OO330_004463"
     CDS             complement(380..>542)
                     /locus_tag="OO330_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885028.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          178 a          125 c           97 g          142 t
ORIGIN      
        1 taaagggata cgattacgat acatatacct tctatgccaa aaaaggtcag aaagtgcatg
       61 tgagtatctc taatgaaggc gctgatactt atctcttcgg gccaggaatc gatgattctg
      121 ttgatttatc cagatattcc ccagaactcg atagtcacgg acaatactca ctccctgctt
      181 ctggcaaata tgagctgcga gtgctgcaaa ctcgaaacga cgcccgtaaa aacaaaacta
      241 aaaaatataa cgtcgatatt caaattaagt aaatgtcaac cgacggggac tatccccgtc
      301 atagacgtta acgccccact cctctctgtc gctcaagagg ttaatgacga atagcagaga
      361 acataatgta aacaaggcat cactcacgac acataaataa catctttgtg gtggtttgat
      421 tgatttttcc actccgctta ccatctacac caaaataacg atcatcacga cataattttt
      481 ctttaacatc gcaaaataca ccgttagcga atgtaaattc gctgggatcg tagccttgta
      541 at
//
LOCUS       DAJTRB010000310          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTRB010000310 DAJTRB010000000
VERSION     DAJTRB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.322"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..>539)
                     /locus_tag="OO330_004464"
     rRNA            complement(<1..>539)
                     /locus_tag="OO330_004464"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          101 a          181 c          120 g          137 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcagaccag ctagggatcg tcgcctaggt gagccgttac cccacctac
//
LOCUS       DAJTRB010000311          537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTRB010000311 DAJTRB010000000
VERSION     DAJTRB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.323"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>537
                     /locus_tag="OO330_004465"
     CDS             <1..>537
                     /locus_tag="OO330_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCR5885029.1"
                     /translation="GRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGM
                     PAINAKRVYRLMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGE
                     RLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNG
                     SCYRANETRQFARMLGLEP"
BASE COUNT          116 a          136 c          167 g          118 t
ORIGIN      
        1 tggccgccgc agtcgtcaca ctgatgatac ggatgtgctt ctccgtatac accatgttat
       61 cggagagctg cccacgtatg gttatcgtcg ggtatgggcg ctgcttcgca gacaggcaga
      121 acttgatggt atgcctgcga tcaatgccaa acgtgtttac cggctcatgc gccagaatgc
      181 gctgttgctt gagcgaaaac ctgctgtacc gccatcgaaa cgggcacata caggcagagt
      241 ggccgtgaaa gaaagcaatc agcgatggtg ctctgacggg ttcgagttct gctgtgataa
      301 cggagagaga ctgcgtgtca cgttcgcgct ggactgctgt gatcgtgagg cactgcactg
      361 ggcggtgact accggcggct tcaacagtga aacagtacag gacgtcatgc tgggagcggt
      421 ggaacgccgc ttcggcaacg atcttccgtc gtctccagtg gagtggctga cggataatgg
      481 ttcatgctac cgggctaatg aaacacgcca gttcgcccgg atgttgggac ttgaacc
//
LOCUS       DAJTRB010000312          536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTRB010000312 DAJTRB010000000
VERSION     DAJTRB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.324"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            78..143
                     /locus_tag="OO330_004466"
                     /pseudo
     CDS             78..143
                     /locus_tag="OO330_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            371..>536
                     /locus_tag="OO330_004467"
     CDS             371..>536
                     /locus_tag="OO330_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5885030.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRT"
BASE COUNT          169 a          113 c          124 g          130 t
ORIGIN      
        1 aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac tgggacctcg
       61 cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata
      121 ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga agatcgtcca
      181 ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac gaacttcgac
      241 gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata aacaatatca
      301 ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac tacagttaca
      361 ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta aaatgccaaa
      421 cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac gttatgacga
      481 tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac gcaccg
//
LOCUS       DAJTRB010000313          514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTRB010000313 DAJTRB010000000
VERSION     DAJTRB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.325"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(25..297)
                     /locus_tag="OO330_004468"
     CDS             complement(25..297)
                     /locus_tag="OO330_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885031.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          114 a          131 c          144 g          125 t
ORIGIN      
        1 cgtttcagcg ggcggggctg aaaatcacca ccacttcggg tcatccgcct tcatctccgc
       61 ttctgtttcg tataaatcaa aacgacggca ggtatggcag aacgtgacgt attcgtacgg
      121 tgaatccggg tacattttgt ggaaggtttc ccagaaacag accgtgcagg agggatgacc
      181 ggtgataaag tctgcaaata cggacttata agggtgatta ttggctctgg cgacggcttt
      241 cagaacctct ttcaccattc tggtgtggac tttctggtgc tccaggttgt gtgacatggg
      301 aactcattct ggatggttac tctgaaagct catattctgc cacaccccga tttgcagccg
      361 ccaggctgcc gtggttcaca gataaaacac tctccaggaa acccggggcg gttcactgcg
      421 tgtctgggtt cgccagcatg agcgggatac cgggggcggt tcacacgaac ataccacaga
      481 cagttgattt tggtacgccc aggcgacgtc cggc
//
LOCUS       DAJTRB010000314          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTRB010000314 DAJTRB010000000
VERSION     DAJTRB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.326"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..163
                     /locus_tag="OO330_004469"
     CDS             <1..163
                     /locus_tag="OO330_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5885032.1"
                     /translation="EDLLLGLISDEDREKLKAYRIYAKSLQAMDFSVITDKTSYNAIE
                     WPASPEISS"
     gene            187..>511
                     /locus_tag="OO330_004470"
     CDS             187..>511
                     /locus_tag="OO330_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5885033.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEE"
BASE COUNT          143 a          111 c          132 g          125 t
ORIGIN      
        1 agaagacctg ttgctggggc taatcagcga cgaagaccgg gaaaaactga aagcgtaccg
       61 catttacgcg aaatcgctgc aggcgatgga tttcagtgtc atcactgata aaacctcata
      121 caacgccatt gaatggcccg cttctccgga aatttcttcc tgatttaatt tatcgcgaga
      181 aaaactatgt ctgtagtgat atcaggtgcg ctgactgatg gcgcaggtat ctccatgtcc
      241 ggataccata ttattctgaa atcccgggta aacaccccgg aagtggtgat gcacactgtt
      301 gctgatgtga tgacaggaaa cgatggtgaa tactgtttcc atgcgcggac tggaaaatat
      361 ggtgtgtatc tgaaacagga ctggcacaac gagtacaacg ttggcgacat tgctgtatat
      421 gaggactcaa agcccggtac gctgaatgac tttctgattg ctcctgatga gggcgacctg
      481 aaaccagatg tcgtcaaacg ctttgaggaa a
//
LOCUS       DAJTRB010000315          507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTRB010000315 DAJTRB010000000
VERSION     DAJTRB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.327"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..238
                     /locus_tag="OO330_004471"
     CDS             <1..238
                     /locus_tag="OO330_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885034.1"
                     /translation="LNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGR
                     NALWIELEGVEYAFPDPQTQNETQQAGYDENIPV"
     gene            289..477
                     /locus_tag="OO330_004472"
     CDS             289..477
                     /locus_tag="OO330_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HCR5885035.1"
                     /translation="MYKITATIEKEGGSPTSWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
BASE COUNT          188 a           98 c          109 g          112 t
ORIGIN      
        1 gctcaacaag caccatgggg gcaccagtaa cgacgccaaa cgcgcaaaag ctgatttgct
       61 ggaaaaaaca ttcacctccc gctcatggga aagaatcaaa ggtatggact ggccgacaat
      121 caaagctggt cgtaatgcac tatggattga actggaaggc gttgaatatg cattcccgga
      181 tccacaaaca caaaacgaaa cgcaacaagc aggatacgat gagaatattc cggtgtaaaa
      241 ccaagtgcat tttgaacacg gtttttgaaa atattaatgg aggtgttcat gtataaaatt
      301 acagccacta ttgaaaagga aggtggttct cctaccagct ggacaagata ttcaaaatct
      361 aaactaacga aatcagaatg cgaaaaaatg ctctcaggga aaaaagaagc aggcgtttcc
      421 agagagcaga aagtaaaact gataaatttt aattgcgaga aacttctgtc ctcgtgagtt
      481 gcattatatc aaaattagaa cttcata
//
LOCUS       DAJTRB010000316          494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTRB010000316 DAJTRB010000000
VERSION     DAJTRB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.328"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            210..>494
                     /locus_tag="OO330_004473"
     CDS             210..>494
                     /locus_tag="OO330_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5885036.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPA"
BASE COUNT          161 a           86 c           76 g          171 t
ORIGIN      
        1 tgaattaatg tattaaatta atattgtgaa tatttataaa ttcctaaaaa ataagtatcg
       61 tcattgtgaa agctactaaa tttcagtaag ttaattccca gcctttaaat ttaatgttaa
      121 ctaaaaatgc ttttttatat tgacttacaa taaatctaca tctctaatac accaactttt
      181 ttgaaacgat attgtaagat caaaattata tggtggcgaa aattaatctc ttattacggc
      241 ttacgccgct tttattcgtg ttcgctcctt ttttggcaca gtcaaatatg actgtttatc
      301 ctatgcctgt ttccattaat agtcagggtg aaggcaacgt tcgcgtaata tctaaaagta
      361 atgaagttca gtacatcaag gcgacagtat tccgtatcga taatccatcg acgcctcagg
      421 aaaatgaagt tgagattaaa tccggtgatg caaatcatct ggttgttatg ccacctaaat
      481 tcgctttacc agcc
//
LOCUS       DAJTRB010000317          487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTRB010000317 DAJTRB010000000
VERSION     DAJTRB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.329"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
BASE COUNT          167 a           92 c          104 g          124 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 ggggggg
//
LOCUS       DAJTRB010000318          482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTRB010000318 DAJTRB010000000
VERSION     DAJTRB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.330"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>482
                     /gene="ospC4"
                     /locus_tag="OO330_004474"
     CDS             <1..>482
                     /gene="ospC4"
                     /locus_tag="OO330_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5885037.1"
                     /translation="NQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNI
                     DPVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKK
                     MAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGA
                     "
BASE COUNT          187 a           58 c           88 g          149 t
ORIGIN      
        1 gtaatcaggg gaaaactgat gttccaatat ttaacactaa agatatgagg cttggaattg
       61 ggttgcatct tattgatttt ataagaaaga gtaaagatca aggattcaga gagttttgtt
      121 ataacaaaaa tattgatcct gttagtctgg atagaattat aaactttgtg tttcagcttg
      181 agtatcatat accaagaatg ctaagtacag ataacttcaa aaaaataaaa ctcagagaca
      241 tatctttgga ggatgcgatt aaagcatcta attatgaaga gattaacaat aaagtgactg
      301 ataaaaaaat ggctcatcag gcccttgctt attctttagg caataaaaaa gctgatatag
      361 cactttattt actatctaaa tttaatttta caaaacaaga tgtcgcagag atggagaaaa
      421 tgaaaaacaa tagatattgt aatctgtatg atgttgaata cttacttagt aaagatggtg
      481 cc
//
LOCUS       DAJTRB010000319          479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTRB010000319 DAJTRB010000000
VERSION     DAJTRB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.331"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..>479)
                     /gene="capU"
                     /locus_tag="OO330_004475"
     CDS             complement(<1..>479)
                     /gene="capU"
                     /locus_tag="OO330_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5885038.1"
                     /translation="AFEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHS
                     GHDSNIVGLVRFFTWKHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQE
                     GCRTRVTVVPPGFDFQELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
BASE COUNT          150 a          109 c           91 g          129 t
ORIGIN      
        1 aaattcgtgc cctttttccg ggcgcaacat tcctacctga gcaattacgg ggcatcctct
       61 tcgggacgcc agccaggaaa gaacactcgg aggcaaactg tttcgggaat caacgtataa
      121 ttcctggaaa tcaaagccag gaggcacaac agtaacccga gttcgacatc cttcctgctc
      181 cagatgtgtc ttcatatttg ttccgggaac aatcacctca tcgcaaaaat gatttattga
      241 aaaaaatttt gtttttcgtg tcaaatatgt cttttgcctg ataattctga atggatgttt
      301 ccaggtaaaa aaccgtacca aaccaacaat attactatca tgtccactgt gacaaacgat
      361 tgcatcgggc tgaaaactat gaactattcc gagtaatctc catacagtag gaatgtgaag
      421 actgtttcga aataacgcga atgtgatatc aattccaaac ttactggctt caaaagcaa
//
LOCUS       DAJTRB010000320          475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTRB010000320 DAJTRB010000000
VERSION     DAJTRB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.332"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>475
                     /locus_tag="OO330_004476"
     CDS             <1..>475
                     /locus_tag="OO330_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5885039.1"
                     /translation="LWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKII
                     ALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFG
                     RRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT"
BASE COUNT          106 a          106 c          118 g          145 t
ORIGIN      
        1 cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag caacataatc
       61 aagcggccgc gaaaaaacgc atccctgtta tcgaagctct gttacgacat cccggtgctt
      121 tcctgaagat tattgcacta cgactgtgcg agttgctgac gatgtacatt gttactgcct
      181 ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg cgaacttttc cttaatattg
      241 gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg cttgccgatc
      301 gttttggtcg gcgcagggtt tatatcacag gcgcgttgat cggaacgttg agcgcatttc
      361 ctttctttat ggcgcttgaa gcacaatcta ttttctggat agttttcttc tccataatgc
      421 tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca accgatgttt accga
//
LOCUS       DAJTRB010000321          465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTRB010000321 DAJTRB010000000
VERSION     DAJTRB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.333"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..>465)
                     /gene="ospC4"
                     /locus_tag="OO330_004477"
     CDS             complement(<1..>465)
                     /gene="ospC4"
                     /locus_tag="OO330_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5885040.1"
                     /translation="VKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGA"
BASE COUNT          151 a           63 c           89 g          162 t
ORIGIN      
        1 gcgccaaaat ctacagtatg atgtgtttta tttagtggga gtttttcttg atgattggaa
       61 atttcgaccc caaaaaaaac aaaatcgtgg ttagagattt ctttaatatc ctcagagtat
      121 gtattcttat gtgtttgtat attttcagac tgaattttat caaaggattt aatattaaga
      181 atattatttg acagattaga gttactttga tgtctgaagt taaaattaga attaataata
      241 atagaaagta agtgacgttc gtctgaagaa agaggatgaa ctttttgtat tatctcagag
      301 atatagtgcc tggacacctt atcgagatgc tcaagtgacc gaaatgatga ccgtgctcca
      361 gatggcgctt caatagaaag atttagtgat ttgaaactgg tcccttgaga aaacattttg
      421 ctataacttt gcgctgctat agacttcctt aaaaagtttt tcaca
//
LOCUS       DAJTRB010000322          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTRB010000322 DAJTRB010000000
VERSION     DAJTRB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.334"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(238..463)
                     /locus_tag="OO330_004478"
                     /pseudo
     CDS             complement(238..463)
                     /locus_tag="OO330_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          150 a          116 c           78 g          119 t
ORIGIN      
        1 aatacctgat tgatgtaagt tcggattctt agctgcttca gggtttaaat tatcaagagc
       61 aaaatttacc aactcagcat aaaggacaac tctaccgact ttatttagcg caccacctga
      121 agaaccagcc ttgccagctt tagctttatt aacagcgtca ccgaatgaag taacagcgtt
      181 tgcaaggttt aacaagcctc ctgctacatt tgcagccttt tgagcagtaa cagccacagc
      241 aagcatcttc agaccatcaa ttaacccacc agataaacca tcaacaattt tcctctgagt
      301 gtcatttagt ggacggtaag ctaatgtcac ctcatcaaag aacgccctta ccctgtaact
      361 aatatcggtc aatgaatcga caccatgtga catagaagtc atgagactac caagagattc
      421 accaagactc ttaccacttc caccaagagc gtttaaaaga ttg
//
LOCUS       DAJTRB010000323          451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTRB010000323 DAJTRB010000000
VERSION     DAJTRB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.335"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            53..>451
                     /locus_tag="OO330_004479"
     CDS             53..>451
                     /locus_tag="OO330_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5885041.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDS"
BASE COUNT          119 a           96 c          110 g          126 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag t
//
LOCUS       DAJTRB010000324          448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTRB010000324 DAJTRB010000000
VERSION     DAJTRB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.336"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            90..371
                     /locus_tag="OO330_004480"
     CDS             90..371
                     /locus_tag="OO330_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885042.1"
                     /translation="MNPMLDYCFTSAPQQTITHTGIKNLLWIIKKNLQQKGDPDLIPC
                     AYGLCDRQVSPDKAYTAELIYMHGTMMRKKKRKYCSEICAEKDQMAHEL"
BASE COUNT          159 a           89 c           88 g          112 t
ORIGIN      
        1 acagggatgg actcttgtta accggggcgc aattaaaaat atgactgtgg aacaattact
       61 ttctttaaaa taaaaatgga gctacggata tgaaccctat gcttgattat tgttttacct
      121 ctgcacctca gcagaccata acacacaccg gaatcaaaaa tttgttatgg ataatcaaaa
      181 aaaacctcca gcagaaaggt gatcccgatt taattccctg tgcttatggc ctgtgtgaca
      241 gacaagtaag ccctgataag gcttacactg ctgaacttat ttacatgcac ggaaccatga
      301 tgaggaaaaa gaaacgcaaa tattgtagtg aaatctgcgc cgaaaaagac cagatggcac
      361 acgaacttta attaactgac tattcgaaac tgaatttatg ccagcaatgg cagggattcg
      421 ctcaacctta attaaggaga aaaacatg
//
LOCUS       DAJTRB010000325          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTRB010000325 DAJTRB010000000
VERSION     DAJTRB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.337"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            28..447
                     /locus_tag="OO330_004481"
                     /pseudo
     CDS             28..447
                     /locus_tag="OO330_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          114 a          107 c          129 g           97 t
ORIGIN      
        1 gtttttagac tagtcattgg agaacagatg attgatgtct tagggccgga gaaacgcaga
       61 cggcgtacca cacaggaaaa gatcgcaatt gttcagcaga gctttgaacc ggggatgacg
      121 gtctccctcg ttgcccggca acatggtgta gcagccagcc agttatttct ctggcgtaag
      181 caataccagg aaggaagtct tactgctgtg gccgccggag aacaggttgt tcctgcctct
      241 gaacttgctg ccgccatgaa gcagattaaa gaactccagc gcctgctcgg caagaaaacg
      301 atggaaaatg aactcctcaa agaagccgtt gaatatggac gggcaaaaaa gtggatagcg
      361 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
      421 cgcgtgcgca gttgcacgtc attctca
//
LOCUS       DAJTRB010000326          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTRB010000326 DAJTRB010000000
VERSION     DAJTRB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.338"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            1..433
                     /locus_tag="OO330_004482"
                     /pseudo
     CDS             1..433
                     /locus_tag="OO330_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          120 a          109 c          115 g           89 t
ORIGIN      
        1 gaagcgggct gcttttcccc gattcaaaaa gcggtgacag aatgatgcat tccgctaccc
       61 gcagggcgtt aagctcgatc aggaaaacag ccgtattttt ctgccgaaac tgggctggat
      121 gcgctaccgg aatagccgtc aggtcacggg tgttgtgaaa aatgtcactg tcagccagtc
      181 ctgcggtacg tggtacatca gtattcagac agaaagtgaa gtatccactc cggctcaccc
      241 ttcagcatca atggtcgggc tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg
      301 cacagtcttt gagcctgtaa acagttttca gaaaaaccag aagacgctgg cgagacttca
      361 gcgacagtta agccgcaagg tcaaattcag caacaactgg cagaagcaga aacgcaaaat
      421 acagcgactg cat
//
LOCUS       DAJTRB010000327          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTRB010000327 DAJTRB010000000
VERSION     DAJTRB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.339"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>431
                     /locus_tag="OO330_004483"
     CDS             <1..>431
                     /locus_tag="OO330_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5885043.1"
                     /translation="LSLVPYCPWQHIVFTLPCQYWSLVFHNRWLLAEMSRIAADVILE
                     ICHQADVEPGTFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMSMWR
                     YRITRLLSRKYPDLVMPDALAAEGSSKREWNRFLDTHYRRG"
BASE COUNT           94 a          115 c          128 g           94 t
ORIGIN      
        1 ctgagcctgg tcccctactg cccgtggcag catattgtgt tcacacttcc ctgccagtac
       61 tggtccctgg tgttccacaa ccggtggtta ctggcagaga tgagccgcat tgcagcggat
      121 gtgatactgg aaatctgcca tcaggcagat gtggagccgg ggacattcac ggtgatccac
      181 acatgggggc gtgaccagca gtggcatccg catatccatt tatcgacaac tgccggtggt
      241 gtgacgtcgg gccacacctg gaaaaatctt catttttacg cccgtaaggt gatgagcatg
      301 tggcgctacc ggataacacg gttgctgtca cggaaatacc ctgacctggt gatgccggat
      361 gcgctggcag cagaaggaag cagtaaacgg gaatggaatc gcttcctgga cacgcattac
      421 cgccgtggct g
//
LOCUS       DAJTRB010000328          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTRB010000328 DAJTRB010000000
VERSION     DAJTRB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.340"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            211..>431
                     /locus_tag="OO330_004484"
     CDS             211..>431
                     /locus_tag="OO330_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5885044.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNA"
BASE COUNT          136 a          104 c           59 g          132 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctctctggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg c
//
LOCUS       DAJTRB010000329          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTRB010000329 DAJTRB010000000
VERSION     DAJTRB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.341"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            22..424
                     /locus_tag="OO330_004485"
                     /pseudo
     CDS             22..424
                     /locus_tag="OO330_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          141 a          103 c           95 g           85 t
ORIGIN      
        1 cagaacaaca caggtgctcc aatgaaaaaa agaaatttta gcgcagagtt taaacgcgaa
       61 tccgctcaac tggttgttga ccagaaatac acggtggcag atgccgccaa agctatggat
      121 gttggccttt ccacaatgac aagatgggtc aaacaactgc gtgatgagcg tcagggcaaa
      181 acaccaaaag cctcccccat taccccggaa caaattgaaa tccgtgagct caggaaaaag
      241 ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct cttgatgtca
      301 gactccctga acagttctcg ataatcggga aactcagagc gcattatcct gtggtcacac
      361 tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac cgtcctgaaa
      421 aacc
//
LOCUS       DAJTRB010000330          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTRB010000330 DAJTRB010000000
VERSION     DAJTRB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.343"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            27..418
                     /locus_tag="OO330_004486"
                     /pseudo
     CDS             27..418
                     /locus_tag="OO330_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           96 a          110 c          108 g          104 t
ORIGIN      
        1 tatgatggtg attttaaggt gcttgcgtgg cttccatttc catcagatgt ccttcctgct
       61 ccgctactga aggcgtggtg cgtaacggca aaagcactgc cggacatcag cgctatctct
      121 gctctcattg ccgtaaaaca tggcaactac agttcactta caccgcctct cagcccggta
      181 cgcaccagaa aatcattgat atggccatga atggcgtcgg atgtcgcgcc agtgcacgca
      241 ttatgggcgt tggcctcaac acggttttac gtcacttaaa aaactcaggc cgcagtcggt
      301 aacctcgcgc atacaaccgg gcagtgatgt gattgtctgc gctgaaatgg acgaacattg
      361 gggctacgtc ggtgctaaat cacgtcagcg ctggctgttt tacgcgtatg acaggata
//
LOCUS       DAJTRB010000331          404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTRB010000331 DAJTRB010000000
VERSION     DAJTRB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.344"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>404
                     /locus_tag="OO330_004487"
     CDS             <1..>404
                     /locus_tag="OO330_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5885045.1"
                     /translation="VTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSP
                     DSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWD
                     NSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           95 a           97 c          133 g           79 t
ORIGIN      
        1 gtgaccgagc caaatcaggt gtggtgcggt gatgtgacct atatctggac gggtaagcgc
       61 tgggcgtacc tcgccgttgt tctcgacctg ttcgcaagaa aaccagtggg ctgggccatg
      121 tcgttctcgc cggacagcag gctcaccatg aaagcgctgg aaatggcatg ggaaacccgt
      181 ggtaagcccg gcggggtgat gttccacagc gatcagggca gtcattatac gagcaggcag
      241 ttccggcagt tattgtggcg ataccagatc agacagagta tgagccggcg cggaaactgc
      301 tgggataaca gcccaatgga acgcttcttc aggagtctga agaacgaatg gatgccggtg
      361 gtgggttacg taagcttcag cgaggcagct cacgccataa cgga
//
LOCUS       DAJTRB010000332          400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTRB010000332 DAJTRB010000000
VERSION     DAJTRB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.345"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>400
                     /locus_tag="OO330_004488"
     CDS             <1..>400
                     /locus_tag="OO330_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5885046.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRPAL"
BASE COUNT           84 a          142 c          101 g           73 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc cggcactcca
//
LOCUS       DAJTRB010000333          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTRB010000333 DAJTRB010000000
VERSION     DAJTRB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.346"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(43..330)
                     /locus_tag="OO330_004489"
     CDS             complement(43..330)
                     /locus_tag="OO330_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885047.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT          107 a           98 c           82 g          109 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgtctttacc tttgttgcaa taaaaagccc
      361 cgcgaatgcg aggctaaatc ctggtgtttg tgatga
//
LOCUS       DAJTRB010000334          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTRB010000334 DAJTRB010000000
VERSION     DAJTRB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.347"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..178)
                     /locus_tag="OO330_004490"
     CDS             complement(<1..178)
                     /locus_tag="OO330_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5885048.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
BASE COUNT           94 a           96 c           95 g          110 t
ORIGIN      
        1 ccctgggtac tttcttcatg aaactgcggt gttcgagttt gcgatatttc ttacagggga
       61 acgcaagccc ctccgcctcc gctttggatt tccgatcagc attaatgtaa tccaccatct
      121 caagactgct catggtcggg tattcgccag aaggaagaac ggttaatgat ttgttcatgt
      181 cggtatctcc tttagaaagt tgagcctgtt cgcacagaaa agccgtcccc gagaggtcct
      241 cacctatacg gcagttctca ggctcagctt tctgaaagac tcgggattgt tacgcgctgc
      301 gatgcgcggt ttactgcgga catataaaag ccccgcaagt gcgaggctca ttaaatggac
      361 tttgtgattt gcaaaaaaat ttatttcagg cactg
//
LOCUS       DAJTRB010000335          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTRB010000335 DAJTRB010000000
VERSION     DAJTRB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.348"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..333)
                     /locus_tag="OO330_004491"
     CDS             complement(<1..333)
                     /locus_tag="OO330_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5885049.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQA"
BASE COUNT          103 a           82 c          102 g          107 t
ORIGIN      
        1 tgcctgctta gccagacgga ccttaaattc acggctatag ctggttcgcc gttcttttcg
       61 ccatgagcct tctctgattt gaggctctgt taattccttc tttctgttgg cataaaggat
      121 ggcgtcaagt tgagcgaatg aaactgaatc gggcaatggc catgcgatac cggatgcaag
      181 aaatcgctga aaaagcgtat gtattgtgga atgactgaga cccagacgct gagcgatggc
      241 ccggatggtc agtttatctt caaatcttaa acgcagggca tcaggcaaat aagaacggaa
      301 gcagggaata tcttttgttg tctgggaatt catcgttcgt gtccatcaat atagatgggc
      361 gcgattgttg ccagacagga caattttcac aaga
//
LOCUS       DAJTRB010000336          379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTRB010000336 DAJTRB010000000
VERSION     DAJTRB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.349"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..336
                     /locus_tag="OO330_004492"
     CDS             <1..336
                     /locus_tag="OO330_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5885050.1"
                     /translation="NSSIADWFNGTTNKIANFQEPMNNFVNNTQSTPSAITGDMVNSN
                     AQLAMAVSELGRKVDALTAETKKPLSVNITGEATVRPDAPSFFSFNQSANDQFTQDML
                     LSSSYPEEE"
BASE COUNT          130 a           74 c           68 g          107 t
ORIGIN      
        1 aatagttcta tcgctgactg gtttaatggt acaactaata aaattgctaa cttccaagaa
       61 ccgatgaata acttcgtaaa taacacacaa tcaactccat cagctatcac aggtgacatg
      121 gttaatagca atgctcaatt agctatggct gtatcagagt taggaagaaa ggtagacgct
      181 ttaacagcag aaactaagaa gcctttatca gttaatatca ctggtgaagc aactgttaga
      241 ccggatgcac catctttctt tagcttcaat caatcagcta atgaccaatt cacgcaggat
      301 atgctcttaa gttcaagcta tccagaggaa gaatgatttc ttatatatcc gtgtgctacg
      361 tgaaatattc tgaacagat
//
LOCUS       DAJTRB010000337          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTRB010000337 DAJTRB010000000
VERSION     DAJTRB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.350"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            260..318
                     /locus_tag="OO330_004493"
                     /pseudo
     CDS             260..318
                     /locus_tag="OO330_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           99 a           83 c          105 g           88 t
ORIGIN      
        1 aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg
       61 attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc
      121 gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc
      181 atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc
      241 cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttggtt gttactcgta
      301 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgtgc
      361 ctttacttta ggttg
//
LOCUS       DAJTRB010000338          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTRB010000338 DAJTRB010000000
VERSION     DAJTRB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.351"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
BASE COUNT          103 a           70 c           74 g          123 t
ORIGIN      
        1 ttttggggtt tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat
       61 gactgcgtat ttgctactaa atagtttctt tattttttcc acgatgcccc caggtttata
      121 agtacaaacg gttatatcca catagagaca aaaatattaa tctgataaat atttatttca
      181 cgccgtatat ttgattgttt aatgtgcaag tacaatgact tttatttttt gttgtgtata
      241 taatcaaata tatggttatt tttcaccctg cgtattcagc acgcaacaaa aaacccgcca
      301 aagcgggttt agtgcgggtg cgttgaggat gcctgacaca tgagaggtgg cgagggattt
      361 ctcccccgcc
//
LOCUS       DAJTRB010000339          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTRB010000339 DAJTRB010000000
VERSION     DAJTRB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.352"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..330)
                     /locus_tag="OO330_004494"
     CDS             complement(<1..330)
                     /locus_tag="OO330_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885051.1"
                     /translation="MPVHLSNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIP
                     LNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRN
                     SADNVDGV"
BASE COUNT           85 a           97 c           79 g           96 t
ORIGIN      
        1 cacaccgtca acgttatccg ctgagttacg gccggtatcc gatatcgcca tccctgacag
       61 cccgtgtccc gaaggtgcgc aaaatctcca cgaaacggtg ttactgccgg ggctgtaata
      121 catttcatgg ccataccgta catcctgtac cccctctgtt cgatatttat agcgggcatc
      181 gaagtttccg taatttaacg gaattacctg tccgttaaca gtgaacacta tgctgttatc
      241 cgggtttctc tggctgaaaa aatgccagcc tgaatcatcg ccaagctctg caacaacagg
      301 cctggatgga ttactgaggt gtactggcaa tagcggacac taccatttgt tcttttt
//
LOCUS       DAJTRB010000340          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTRB010000340 DAJTRB010000000
VERSION     DAJTRB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.353"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
BASE COUNT          118 a           90 c           68 g           81 t
ORIGIN      
        1 tcttcgcccc taaataacag catgattcca gtccatgcaa aatctgattt ccagctcaca
       61 cataccgagc gctgtacaaa aaaccgccag ccaaggcagt taagagtgtg ttcccggggt
      121 ttgcttagaa tattttgtaa gttgtccgaa gagatattta caacaccaga atgatgaatc
      181 atcagtcccc tgccagaggt tcactacgta acctgaccga caatgcacca ttctggtgct
      241 gaaaaaagag cactggaact gcaacaaaac aacatcacag aacaggagat ggtaaggagt
      301 cagacattcc cacacaatat tgtgtcagtg caaataacaa ccttcgtctc aaatctc
//
LOCUS       DAJTRB010000341          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTRB010000341 DAJTRB010000000
VERSION     DAJTRB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.354"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="OO330_004495"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="OO330_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5885052.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DAJTRB010000342          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTRB010000342 DAJTRB010000000
VERSION     DAJTRB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.355"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO330_004496"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO330_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5885053.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTRB010000343          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTRB010000343 DAJTRB010000000
VERSION     DAJTRB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.356"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>339
                     /locus_tag="OO330_004497"
     CDS             <1..>339
                     /locus_tag="OO330_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5885054.1"
                     /translation="NEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDVVKRFEEMVA
                     QAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAG
                     EKAQQKSRDNL"
BASE COUNT           91 a           90 c          105 g           53 t
ORIGIN      
        1 caacgagtac aacgttggcg acattgctgt atatgaggac tcaaagcccg gcacgctgaa
       61 tgactttctg attgctcctg atgagggcga cctgaaaccg gatgtcgtca aacgctttga
      121 ggaaatggtg gcgcaggcgc agcagagcgc cggggccgca gccggaaacg cacagcagac
      181 ggcgcaggat gtggcggcag ccgcaggtta tgcccgcgca gcagagcagg ccaaaaatga
      241 cattgatgct gcgctgaccg gcactctgaa aacggctaac catctgtctg aaatcgcagc
      301 agcaggcgaa aaggcacaac agaagtcccg ggataatct
//
LOCUS       DAJTRB010000344          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTRB010000344 DAJTRB010000000
VERSION     DAJTRB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.358"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            69..>333
                     /locus_tag="OO330_004498"
     CDS             69..>333
                     /locus_tag="OO330_004498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR5885055.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVS"
BASE COUNT          103 a           62 c           76 g           92 t
ORIGIN      
        1 tagacagtga gaccatgtgc attggtttta aaaatgccgt ctatgttcat gattgccttg
       61 atttgcatat ggaacaactg gatttagatt attgcggctc aacaggcgtg gttatagaga
      121 atgtaaacgg aggattttct ttctcaaact catggatagc agcagatgcc gatggcactg
      181 aacaatttac ggggatatat tttagaacgc cggcctcaac gcagtcacat aaaattgtca
      241 gtggtgttca tatcaacact gcaaacaaaa acacggctgc aaacaatcaa agtatagcga
      301 tagaacagtc ggcgatcttc gtctttgtaa gtg
//
LOCUS       DAJTRB010000345          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTRB010000345 DAJTRB010000000
VERSION     DAJTRB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.360"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..>325
                     /locus_tag="OO330_004499"
     CDS             <1..>325
                     /locus_tag="OO330_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5885056.1"
                     /translation="LSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTS
                     LGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALMILITLMNSA
                     GGKAS"
BASE COUNT           61 a           84 c           88 g           92 t
ORIGIN      
        1 tgctttccgg tatgtatccg tcttctgtaa tggcggcttc cggtgctccg tttgcaatca
       61 gtgcttcaac tatcctcggt aactgggctg cgccgctggt ttctgcattc accgcctttg
      121 cgtgcctgac ttctctgggc tcctggatga tgttggtagg ccaggcaggt gtacgtgccg
      181 ctaacgacgg taacttcccg aaagtttatg gtgaagtcga cagcaacggt attccgaaaa
      241 aaggtctgct gctggctgca gtgaaaatga ctgccctgat gatcctcatc actctgatga
      301 actctgccgg tggtaaagca tctga
//
LOCUS       DAJTRB010000346          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTRB010000346 DAJTRB010000000
VERSION     DAJTRB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.361"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
BASE COUNT           90 a           60 c           84 g           87 t
ORIGIN      
        1 cggcggggag ctgtttatcc ctgcgagcaa ccaagcggtg gggtatattg ttggatccac
       61 tttgcttatt cctggcggtg ttaacatcag aggagttggt aaggcatcgc aactccgagc
      121 aaaaagcgga cttacaggat ctgtgttaag gctgtcttat gattcagaca ctatcggccg
      181 ttatctgaga aatatacgag taactggtaa taacacctgc aatggtattg acacaaacat
      241 tacagcagaa gactctgtca tcagacaggt ttatggctgg gtatttgata atgtaatggt
      301 gaatgaagtt gaaaccgcat t
//
LOCUS       DAJTRB010000347          314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTRB010000347 DAJTRB010000000
VERSION     DAJTRB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.362"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            135..>314
                     /locus_tag="OO330_004500"
     CDS             135..>314
                     /locus_tag="OO330_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5885057.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHY"
BASE COUNT           87 a           67 c           93 g           67 t
ORIGIN      
        1 tccccacagc cggatgaact aacgttcgtc cggtttgcta ttgagttcct gcctggatac
       61 ttaccgcgaa aacagcacgg ctgacataaa ataaatatgg tctaatgggg aaatttctcg
      121 tggagaggga acagatgcga tatccggttg atgtatacac aggcaagatt caggcttatc
      181 ccgaaggcaa acccagcgca attgccaaaa tccaggttga tggtgagttg atgctgacgg
      241 agctggggct ggaaggtgac gagcaggcgg agaaaaaggt tcacggcggg ccagacagag
      301 cgctgtgtca ttat
//
LOCUS       DAJTRB010000348          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTRB010000348 DAJTRB010000000
VERSION     DAJTRB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.363"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..243)
                     /locus_tag="OO330_004501"
     CDS             complement(<1..243)
                     /locus_tag="OO330_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885058.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVK"
BASE COUNT           89 a           75 c           71 g           70 t
ORIGIN      
        1 tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac cgatgacgat
       61 atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca ccatcccgcc
      121 aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg ctttcttatg
      181 ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg tgtttgagac
      241 cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta tccgtcatgc
      301 ctgag
//
LOCUS       DAJTRB010000349          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTRB010000349 DAJTRB010000000
VERSION     DAJTRB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.denovo.364"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..175)
                     /locus_tag="OO330_004502"
     CDS             complement(<1..175)
                     /locus_tag="OO330_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5885059.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDC"
BASE COUNT           67 a           92 c           84 g           60 t
ORIGIN      
        1 aacagtccgg tgatgaacaa caccactgcg tgtagcccat cagtgtggtc ccgcaggcca
       61 tgatttttgt cactgattca attaccaccg gacggactgc cccttccggt tgtttctcca
      121 gccagttaag ccagcggttt ccctgctgaa aaatatcggc aaaacggggg agcatcagaa
      181 gggcggggcg actccgtccg gccagtgaac cgtgccacac tccgggcagt acataccgcc
      241 ggcgctgata ccggcaagaa tggtcgcaaa ttcccgctcc gtgcagcggg cgatttccgg
      301 ata
//
LOCUS       DAJTRB010000350         5288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.188, whole genome shotgun sequence.
ACCESSION   DAJTRB010000350 DAJTRB010000000
VERSION     DAJTRB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.188"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..1029)
                     /locus_tag="OO330_004503"
     CDS             complement(<1..1029)
                     /locus_tag="OO330_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HCR5885060.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQS"
     gene            complement(1032..1592)
                     /locus_tag="OO330_004504"
     CDS             complement(1032..1592)
                     /locus_tag="OO330_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5885061.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(1718..2068)
                     /locus_tag="OO330_004505"
     CDS             complement(1718..2068)
                     /locus_tag="OO330_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5885062.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(2271..3284)
                     /gene="intI1"
                     /locus_tag="OO330_004506"
     CDS             complement(2271..3284)
                     /gene="intI1"
                     /locus_tag="OO330_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5885063.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3494..4324
                     /locus_tag="OO330_004507"
     CDS             3494..4324
                     /locus_tag="OO330_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5885064.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            4437..5228
                     /gene="aadA1"
                     /locus_tag="OO330_004508"
     CDS             4437..5228
                     /gene="aadA1"
                     /locus_tag="OO330_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5885065.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1252 a         1438 c         1460 g         1138 t
ORIGIN      
        1 tgattgcttg gcgtcgatca gcgcctgacc gatgcgcccg tacagacgta ccttggcgtt
       61 gatggccttg cctgacgcct ggaactgctg ctgatgctta ttcttggcag cgttaaacag
      121 cttacccagg atgcggtcgt gcaggtcgat gatttcgtcg gtgacggtgg ccatgccctc
      181 ggtggccagc gccacgagag tggcgtagcg ccgttgcggc tcgaatttgg ccaggtcggc
      241 gggtgtcatc tggccgccct cgcgggcaat cttgagcagg cggttctggt gaaccagccg
      301 ctcgatgccg gtaggcagat cgagtgcctg ccatgccttg aggcgttcga tgtgttccag
      361 catatgccgc gaatttggct tggccggaga ctggcgcaac caagccaacc aggtcgtctt
      421 gccgttgtcc cggcgcttga gcagatcgtc gaggcggcgg cgatgcgcgt ccgccagtgg
      481 ttcggccaag gcgtcgtaga tgcgccggtt agcacgggtg atcgcctcgg cactcgcccg
      541 ctcgacggcg ttgagggcgg gcagaatgac cgactgccgc cgcaggtgcc cgatcaaggc
      601 gctggccagc acgatgccct tgtcggtttg catcgccagc tcggtcagca tctggacggc
      661 ctgccggtaa tggctcatgg tgaagggccg gaaaccgaac acggtttgca gctcgctcag
      721 gtgctcgcgc cgggtctgct cccgctggcc gtactcgttc cagctttcga cgccgacctt
      781 gagctggtcg gcgaccagct tcaacaaggg cgggaacggt agttcatcga cgcccaggat
      841 gacgccggga aagcgcaggt aacagagctg caccgcgaag cccagccgat tggctggccc
      901 gcgccgctgt cggatgatcg agaggtcggt atcgttgaat gtgtaatgtc ggatcaggtc
      961 gtccttggag tccggcaacg ccagcaggct ttcccgctcg gcggcggaca ggatggaacg
     1021 acgtggcata tttactgatc cgttctcaag tattgataca gggtttcgcg actgattccg
     1081 aattcacgag caagcttggt cttttgctcg ccagcctcga cacgttggcg cagttcggca
     1141 atacgctcag acgacaggga tttcttcctg ccacggtaag ccccgcgttg cttggcgagc
     1201 gcaataccct cgcgctgacg ctcgcggatc agggcgcgct cgaactcggc gaacgcgccc
     1261 atcaccgaga gcatcaggtt cgccatcgga gagtcttcgc cagtaaaact gaggtgttcc
     1321 ttgacgaatt cgatatgcac gccgcgttgt gtcagcgttt gcacgatccg gcgcaaatca
     1381 tcgagattgc gcgccaggcg atccatgcta tgcaccacca cggtgtcgcc ggtgcgggcg
     1441 aagcttatca gcgcttccag ttgcggacgc ttgacatcct tgccggatgc cttgtcgcta
     1501 aaagcgcgat caaccttgac gccttccagt tgccgttccg ggttctggtc gaaggtgctg
     1561 accctgatat acccaatgcg ctgtccagtc atggaattcc ctgcaaaatg tcagggaaga
     1621 ctctatgacc ttcaacgaga tatgtcaata aattcaaaat tcaatcctat cctgacgcaa
     1681 tttacacatg gcatctgaca tcaggttagg gtatgcctca acctgacggc ggcgaatcac
     1741 aagcgtccgg tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg aagcgctgat
     1801 agcactccag cccgcagaaa tgctccacgt actcggcccc ttccggcgtg aaggcggcat
     1861 cgagcgggat ttccttgcag cacacgcagc aactggtgct cggttcattg gcgttcatgg
     1921 tggtgttcct ccattggttg acgaagccga cgaaggccgc cgccggcatc ggcctggcga
     1981 acaggaaacc ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg cagtcgggtg
     2041 tttccacacc ctcggctacc acctccattg tcgttttcag aagacggctg cactgaacgt
     2101 cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac gacgggctgc
     2161 tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga tgcggcaccg
     2221 atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc ctacctctca
     2281 ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc aactttcagc
     2341 acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc ctgcacggtt
     2401 cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag ggtgtgcggt
     2461 gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg ctgaaaggtc
     2521 tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga atgcgtgtgc
     2581 tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt ccgctcaagg
     2641 gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca ccatgcccgt
     2701 gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga
     2761 tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg
     2821 acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac
     2881 aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc
     2941 accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg
     3001 cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc
     3061 gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag
     3121 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     3181 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     3241 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     3301 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     3361 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     3421 aagttgggcg aacccggagc ctcattaatt gttagccgtt aaaattaagc cctttaccaa
     3481 accaatactt attatgaaaa acacaataca tatcaacttc gctatttttt taataattgc
     3541 aaatattatc tacagcagcg ccagtgcatc aacagatatc tctactgttg catctccatt
     3601 atttgaagga actgaaggtt gttttttact ttacgatgca tccacaaacg ctgaaattgc
     3661 tcaattcaat aaagcaaagt gtgcaacgca aatggcacca gattcaactt tcaagatcgc
     3721 attatcactt atggcatttg atgcggaaat aatagatcag aaaaccatat tcaaatggga
     3781 taaaaccccc aaaggaatgg agatctggaa cagcaatcat acaccaaaga cgtggatgca
     3841 attttctgtt gtttgggttt cgcaagaaat aacccaaaaa attggattaa ataaaatcaa
     3901 gaattatctc aaagattttg attatggaaa tcaagacttc tctggagata aagaaagaaa
     3961 caacggatta acagaagcat ggctcgaaag tagcttaaaa atttcaccag aagaacaaat
     4021 tcaattcctg cgtaaaatta ttaatcacaa tctcccagtt aaaaactcag ccatagaaaa
     4081 caccatagag aacatgtatc tacaagatct ggataatagt acaaaactgt atgggaaaac
     4141 tggtgcagga ttcacagcaa atagaacctt acaaaacgga tggtttgaag ggtttattat
     4201 aagcaaatca ggacataaat atgtttttgt gtccgcactt acaggaaact tggggtcgaa
     4261 tttaacatca agcataaaag ccaagaaaaa tgcgatcacc attctaaaca cactaaattt
     4321 ataaaaaatc taatggcaaa atcgcccaac ccttcaatca agtcgggacg gccaaaagca
     4381 agcttttggc tcccctcgct ggcgctcggc gccccttatt tcaaacgtta aacatcatga
     4441 gggaagtggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     4501 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     4561 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     4621 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     4681 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     4741 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     4801 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     4861 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     4921 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     4981 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     5041 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     5101 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     5161 gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg
     5221 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     5281 gcgttaga
//
LOCUS       DAJTRB010000351         5285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.189, whole genome shotgun sequence.
ACCESSION   DAJTRB010000351 DAJTRB010000000
VERSION     DAJTRB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.189"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..1029)
                     /locus_tag="OO330_004509"
     CDS             complement(<1..1029)
                     /locus_tag="OO330_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HCR5885066.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQS"
     gene            complement(1032..1592)
                     /locus_tag="OO330_004510"
     CDS             complement(1032..1592)
                     /locus_tag="OO330_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5885067.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(1718..2068)
                     /locus_tag="OO330_004511"
     CDS             complement(1718..2068)
                     /locus_tag="OO330_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5885068.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(2271..3284)
                     /gene="intI1"
                     /locus_tag="OO330_004512"
     CDS             complement(2271..3284)
                     /gene="intI1"
                     /locus_tag="OO330_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5885069.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3494..4324
                     /locus_tag="OO330_004513"
     CDS             3494..4324
                     /locus_tag="OO330_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5885070.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            4437..5225
                     /gene="aadA1"
                     /locus_tag="OO330_004514"
     CDS             4437..5225
                     /gene="aadA1"
                     /locus_tag="OO330_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5885071.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1249 a         1438 c         1460 g         1138 t
ORIGIN      
        1 tgattgcttg gcgtcgatca gcgcctgacc gatgcgcccg tacagacgta ccttggcgtt
       61 gatggccttg cctgacgcct ggaactgctg ctgatgctta ttcttggcag cgttaaacag
      121 cttacccagg atgcggtcgt gcaggtcgat gatttcgtcg gtgacggtgg ccatgccctc
      181 ggtggccagc gccacgagag tggcgtagcg ccgttgcggc tcgaatttgg ccaggtcggc
      241 gggtgtcatc tggccgccct cgcgggcaat cttgagcagg cggttctggt gaaccagccg
      301 ctcgatgccg gtaggcagat cgagtgcctg ccatgccttg aggcgttcga tgtgttccag
      361 catatgccgc gaatttggct tggccggaga ctggcgcaac caagccaacc aggtcgtctt
      421 gccgttgtcc cggcgcttga gcagatcgtc gaggcggcgg cgatgcgcgt ccgccagtgg
      481 ttcggccaag gcgtcgtaga tgcgccggtt agcacgggtg atcgcctcgg cactcgcccg
      541 ctcgacggcg ttgagggcgg gcagaatgac cgactgccgc cgcaggtgcc cgatcaaggc
      601 gctggccagc acgatgccct tgtcggtttg catcgccagc tcggtcagca tctggacggc
      661 ctgccggtaa tggctcatgg tgaagggccg gaaaccgaac acggtttgca gctcgctcag
      721 gtgctcgcgc cgggtctgct cccgctggcc gtactcgttc cagctttcga cgccgacctt
      781 gagctggtcg gcgaccagct tcaacaaggg cgggaacggt agttcatcga cgcccaggat
      841 gacgccggga aagcgcaggt aacagagctg caccgcgaag cccagccgat tggctggccc
      901 gcgccgctgt cggatgatcg agaggtcggt atcgttgaat gtgtaatgtc ggatcaggtc
      961 gtccttggag tccggcaacg ccagcaggct ttcccgctcg gcggcggaca ggatggaacg
     1021 acgtggcata tttactgatc cgttctcaag tattgataca gggtttcgcg actgattccg
     1081 aattcacgag caagcttggt cttttgctcg ccagcctcga cacgttggcg cagttcggca
     1141 atacgctcag acgacaggga tttcttcctg ccacggtaag ccccgcgttg cttggcgagc
     1201 gcaataccct cgcgctgacg ctcgcggatc agggcgcgct cgaactcggc gaacgcgccc
     1261 atcaccgaga gcatcaggtt cgccatcgga gagtcttcgc cagtaaaact gaggtgttcc
     1321 ttgacgaatt cgatatgcac gccgcgttgt gtcagcgttt gcacgatccg gcgcaaatca
     1381 tcgagattgc gcgccaggcg atccatgcta tgcaccacca cggtgtcgcc ggtgcgggcg
     1441 aagcttatca gcgcttccag ttgcggacgc ttgacatcct tgccggatgc cttgtcgcta
     1501 aaagcgcgat caaccttgac gccttccagt tgccgttccg ggttctggtc gaaggtgctg
     1561 accctgatat acccaatgcg ctgtccagtc atggaattcc ctgcaaaatg tcagggaaga
     1621 ctctatgacc ttcaacgaga tatgtcaata aattcaaaat tcaatcctat cctgacgcaa
     1681 tttacacatg gcatctgaca tcaggttagg gtatgcctca acctgacggc ggcgaatcac
     1741 aagcgtccgg tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg aagcgctgat
     1801 agcactccag cccgcagaaa tgctccacgt actcggcccc ttccggcgtg aaggcggcat
     1861 cgagcgggat ttccttgcag cacacgcagc aactggtgct cggttcattg gcgttcatgg
     1921 tggtgttcct ccattggttg acgaagccga cgaaggccgc cgccggcatc ggcctggcga
     1981 acaggaaacc ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg cagtcgggtg
     2041 tttccacacc ctcggctacc acctccattg tcgttttcag aagacggctg cactgaacgt
     2101 cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac gacgggctgc
     2161 tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga tgcggcaccg
     2221 atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc ctacctctca
     2281 ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc aactttcagc
     2341 acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc ctgcacggtt
     2401 cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag ggtgtgcggt
     2461 gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg ctgaaaggtc
     2521 tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga atgcgtgtgc
     2581 tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt ccgctcaagg
     2641 gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca ccatgcccgt
     2701 gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga
     2761 tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg
     2821 acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac
     2881 aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc
     2941 accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg
     3001 cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc
     3061 gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag
     3121 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     3181 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     3241 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     3301 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     3361 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     3421 aagttgggcg aacccggagc ctcattaatt gttagccgtt aaaattaagc cctttaccaa
     3481 accaatactt attatgaaaa acacaataca tatcaacttc gctatttttt taataattgc
     3541 aaatattatc tacagcagcg ccagtgcatc aacagatatc tctactgttg catctccatt
     3601 atttgaagga actgaaggtt gttttttact ttacgatgca tccacaaacg ctgaaattgc
     3661 tcaattcaat aaagcaaagt gtgcaacgca aatggcacca gattcaactt tcaagatcgc
     3721 attatcactt atggcatttg atgcggaaat aatagatcag aaaaccatat tcaaatggga
     3781 taaaaccccc aaaggaatgg agatctggaa cagcaatcat acaccaaaga cgtggatgca
     3841 attttctgtt gtttgggttt cgcaagaaat aacccaaaaa attggattaa ataaaatcaa
     3901 gaattatctc aaagattttg attatggaaa tcaagacttc tctggagata aagaaagaaa
     3961 caacggatta acagaagcat ggctcgaaag tagcttaaaa atttcaccag aagaacaaat
     4021 tcaattcctg cgtaaaatta ttaatcacaa tctcccagtt aaaaactcag ccatagaaaa
     4081 caccatagag aacatgtatc tacaagatct ggataatagt acaaaactgt atgggaaaac
     4141 tggtgcagga ttcacagcaa atagaacctt acaaaacgga tggtttgaag ggtttattat
     4201 aagcaaatca ggacataaat atgtttttgt gtccgcactt acaggaaact tggggtcgaa
     4261 tttaacatca agcataaaag ccaagaaaaa tgcgatcacc attctaaaca cactaaattt
     4321 ataaaaaatc taatggcaaa atcgcccaac ccttcaatca agtcgggacg gccaaaagca
     4381 agcttttggc tcccctcgct ggcgctcggc gccccttatt tcaaacgtta aacatcatga
     4441 gggaagtggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     4501 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     4561 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     4621 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     4681 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     4741 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     4801 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     4861 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     4921 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     4981 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     5041 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     5101 ccgtcatact tgaagctagg caggcttatc ttggacaaga agatcgcttg gcctcgcgcg
     5161 cagatcagtt ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca
     5221 aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     5281 ttaga
//
LOCUS       DAJTRB010000352         4874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.195, whole genome shotgun sequence.
ACCESSION   DAJTRB010000352 DAJTRB010000000
VERSION     DAJTRB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.195"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            632..1447
                     /gene="sul2"
                     /locus_tag="OO330_004515"
     CDS             632..1447
                     /gene="sul2"
                     /locus_tag="OO330_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5885072.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1508..2311
                     /locus_tag="OO330_004516"
                     /note="aph(3'')-Ib"
     CDS             1508..2311
                     /locus_tag="OO330_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5885073.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2311..3147
                     /locus_tag="OO330_004517"
     CDS             2311..3147
                     /locus_tag="OO330_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5885074.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3254..3658)
                     /locus_tag="OO330_004518"
                     /pseudo
     CDS             complement(3254..3658)
                     /locus_tag="OO330_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3702..4091)
                     /locus_tag="OO330_004519"
     CDS             complement(3702..4091)
                     /locus_tag="OO330_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885075.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4309..4749
                     /locus_tag="OO330_004520"
     CDS             4309..4749
                     /locus_tag="OO330_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5885076.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1151 a         1325 c         1273 g         1125 t
ORIGIN      
        1 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tgtccggtaa
       61 ctatcaactt gagtccaacc cggaaagaca cgacaaaacg ccactggcag cagccattag
      121 taacaggagt gcacgagaag atagattaag aggtcttgaa gtagaggcct gactgcggct
      181 acactgaaag aacagttttg gtaatggcgg tctcgtgaac cctgttaccg ggttaagcag
      241 ttccccaact gacttaacct tcgaacaaac cgcctcccca ggcggttttt tcgtttacag
      301 agcaagagat tacgattcat tgaaaaggat ctcaagaaga tcctttgtag attactatca
      361 attaatcttc cgaacatgac accagtcact gttgattacg gttaaaccgc tccgtaacag
      421 gtttccctcg tattggtgac ttgcagcacc aaagaagcaa aaaagataaa tatatctact
      481 tattgagaga tgatatattt atttccaaaa tgctacagat acatcatcat gaaacggatc
      541 accgcaaaca ggttactcgc ctggggattc cctttcgacc cgagcatccg tatgagactc
      601 atgctcgatt attattatta tagaagcccc catgaataaa tcgctcatca ttttcggcat
      661 cgtcaacata acctcggaca gtttctccga tggaggccgg tatctggcgc cagacgcagc
      721 cattgcgcag gcgcgtaagc tgatggccga gggggcagat gtgatcgacc tcggtccggc
      781 atccagcaat cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc gtatcgcgcc
      841 ggtgctggac gcgctcaagg cagatggcat tcccgtctcg ctcgacagtt atcaacccgc
      901 gacgcaagcc tatgccttgt cgcgtggtgt ggcctatctc aatgatattc gcggttttcc
      961 agacgctgcg ttctatccgc aattggcgaa atcatctgcc aaactcgtcg ttatgcattc
     1021 ggtgcaagac gggcaggcag atcggcgcga ggcacccgct ggcgacatca tggatcacat
     1081 tgcggcgttc tttgacgcgc gcatcgcggc gctgacgggt gccggtatca aacgcaaccg
     1141 ccttgtcctt gatcccggca tggggttttt tctgggggct gctcccgaaa cctcgctctc
     1201 ggtgctggcg cggttcgatg aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt
     1261 ttcgcgcaaa tcctttctgc gcgcgctcac aggccgtggt ccgggggatg tcggggccgc
     1321 gacactcgct gcagagcttg ccgccgccgc aggtggagct gacttcatcc gcacacacga
     1381 gccgcgcccc ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat
     1441 tcgttaactg cacattcggg atatttctct atattcgcgc ttcatcagaa aactgaagga
     1501 acctccattg aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt
     1561 cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa
     1621 aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct
     1681 caaaggtcga ggtgtggctt gccccgaggt catcaactgg caggaggaac aggagggtgc
     1741 atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt
     1801 gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga
     1861 tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc
     1921 ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga
     1981 tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgatat
     2041 ggttgtttgc catggtgatc cctgcatgcc gaacttcatg gtggacccta aaactcttca
     2101 atgcacgggt ctgatcgacc ttgggcggct cggaacagca gatcgctatg ccgatttggc
     2161 actcatgatt gctaacgccg aagagaactg ggcagcgcca gatgaagcag agcgcgcctt
     2221 cgctgtccta ttcaatgtat tggggatcga agcccccgac cgcgaacgcc ttgccttcta
     2281 tctgcgattg gaccctctga cttggggttg atgttcatgc cgcctgtttt tcctgctcat
     2341 tggcacgttt cgcaacctgt tctcattgcg gacacctttt ccagcctcgt ttggaaagtt
     2401 tcattgccag acgggactcc tgcaatcgtc aagggattga aacctataga agacattgct
     2461 gatgaactgc gcggggccga ctatctggta tggcgcaatg ggaggggagc agtccggttg
     2521 ctcggtcgtg agaacaatct gatgttgctc gaatatgccg gggagcgaat gctctctcac
     2581 atcgttgccg agcacggcga ctaccaggcg accgaaattg cagcggaact aatggcgaag
     2641 ctgtatgccg catctgagga acccctgcct tctgcccttc tcccgatccg ggatcgcttt
     2701 gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc
     2761 cacgcggcga ttatagccga tcaaatgatg agcaatgcct cggaactgcg tgggctacat
     2821 ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg gctggctggt gatagatccc
     2881 gtcggtctgg tcggtgaagt gggctttggc gccgccaata tgttctacga tccggctgac
     2941 agagacgacc tttgtctcga tcctagacgc attgcacaga tggcggacgc attctctcgt
     3001 gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg gtgcctttcc
     3061 gcagcttgga acgcggatgg agaagaggag caacgcgatc tagctatcgc ggccgcgatc
     3121 aagcaggtgc gacagacgtc atactagata tcaagcgact tctcctatcc cctgggaaca
     3181 catcaatctc accggagaat atcgctggcc aaagccttag cgtaggattc cgccccttcc
     3241 cgcaaacgac cccaaacagg aaacgcagct gaaacgggaa gctcaacacc cactgacgca
     3301 tgggttgttc aggcagtact tcatcaacca gcaaggcggc actttcggcc atccgccgcg
     3361 ccccacagct cgggcagaaa ccgcgacgct tacagctgaa agcgaccagg tgctcggcgt
     3421 ggcaagactc gcagcgaacc cgtagaaagc catgctccag ccgcccgcat tggagaaatt
     3481 cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc ataagcgcag
     3541 cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg ggtcggtggc
     3601 tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca tgaggcatgt
     3661 tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta
     3721 ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccact
     3781 cttacattgt tcgcagtatc taggtacatg ttattgcgtg caccttcatg atgtataatg
     3841 ttattaatga cagcagaaag atcgacagaa ttaatattct tcgtacctgg gacagttatc
     3901 gggtgtcggc cagtgatgag accaaaggat gcagtataag atgaaccact actattatca
     3961 tggccaccat taccaccacc gttaaaacta cctcccgaac ttccagagcc gctatgacca
     4021 tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac cgacagtacc gctatttaac
     4081 tctgtactca taaaattatc ctctttgact tttaaaaaca gcaaaaactg tatttataac
     4141 aatgtgttta tatacagtat tcgaaatact acgcccgtca aaaccacacc gtcaacaggt
     4201 tttttgtgta tttttcacgc ctcacgggca gcctcagacc ttttgtgtta aaacagtaat
     4261 atgttctgct gttacaatac ggtacacaca ggagacactg tcacttttat ggcatggcac
     4321 acagttcaag agacactgaa attaacagga aagtcgcgct cgcaattata tcgtgacatg
     4381 gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc gccgggagtt cgagaccagt
     4441 gaactgattc gggcctatgg cgagctgcgc acttctgaga caccaaaaag gcacaaggag
     4501 ggacactcca aatctgtcgt tagccaggcg gaaaatgctc agctagacgt cattcagcag
     4561 aaactggacg ctctgcagca gactgttacc ctaatgcttg aagacaagat ggcgcgggaa
     4621 gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac aggccgaaat tagccgtctg
     4681 gctgacgcgc tggcacagga aaaaaagcgg ggattctggt cgcggctgtt tcgctccggt
     4741 tcagagtaac agtgcaggtg actctgattt accataacat ctgttatcag caccgcctga
     4801 gaggccctca gggcgtttct gacgggaaac cggtaaaaca ggcaacaaac cacccgaaaa
     4861 cccgccagaa acgc
//
LOCUS       DAJTRB010000353         4510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.202, whole genome shotgun sequence.
ACCESSION   DAJTRB010000353 DAJTRB010000000
VERSION     DAJTRB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.202"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..1222
                     /locus_tag="OO330_004521"
     CDS             <1..1222
                     /locus_tag="OO330_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR5885077.1"
                     /translation="GRVYRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGS
                     GILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHE
                     WRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAH
                     YTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATER
                     AATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPEL
                     MQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELSHGLNVAECMAHSAMARKESR
                     GAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAA
                     DKAEAANNKEKANG"
     gene            1215..1949
                     /gene="frdB"
                     /locus_tag="OO330_004522"
     CDS             1215..1949
                     /gene="frdB"
                     /locus_tag="OO330_004522"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5885078.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1960..2355
                     /gene="frdC"
                     /locus_tag="OO330_004523"
     CDS             1960..2355
                     /gene="frdC"
                     /locus_tag="OO330_004523"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5885079.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2366..2725
                     /gene="frdD"
                     /locus_tag="OO330_004524"
     CDS             2366..2725
                     /gene="frdD"
                     /locus_tag="OO330_004524"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5885080.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2788..3921
                     /locus_tag="OO330_004525"
     CDS             2788..3921
                     /locus_tag="OO330_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5885081.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4010..>4510
                     /gene="blc"
                     /locus_tag="OO330_004526"
     CDS             4010..>4510
                     /gene="blc"
                     /locus_tag="OO330_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5885082.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVS"
BASE COUNT         1096 a         1179 c         1212 g         1023 t
ORIGIN      
        1 gggtcgcgtt tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat
       61 ggcgctaagc cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg
      121 tctgccaggt tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt
      181 caacaaaaat ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg
      241 cgagccgaaa aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg
      301 gcacgaatgg cgtaaaggca acaccatctc cacgccgcgt ggtgatgtgg tttatctcga
      361 cctgcgtcac ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc
      421 gaaagcgtac gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgactgcaca
      481 ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt
      541 cgccgtgggt gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc
      601 cctggcggaa ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc
      661 aactgccggt aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg
      721 tctgaaagat ctagttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat
      781 gggcctggca atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac
      841 catcgacaag ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc
      901 cagcgtgttc aacaccgacc tgctctacac cattgaactg agccacggtc tgaacgttgc
      961 tgaatgtatg gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct
     1021 ggacgaaggt tgcaccgagc gtgacgacgt caactttctc aaacacaccc tcgccttccg
     1081 cgatgctgat ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc
     1141 agctaaacgc gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa
     1201 taaggagaag gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc
     1261 cggaagtcga taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct
     1321 cattactgga tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct
     1381 ggtcctgccg tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac
     1441 tggcatgtaa aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta
     1501 acttcccgat tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag
     1561 cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga
     1621 ccccggcgca gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct
     1681 acgccgcgtg cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc
     1741 tggcgcatcg ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt
     1801 tgaacagcca gaacggtgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc
     1861 cgaaacacgt cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact
     1921 ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg
     1981 tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg
     2041 ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg
     2101 tttgccctga aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg
     2161 gttatcgtga tcattaacct gatcactctg acggcagctc tgctgcacac caaaacctgg
     2221 tttgaactgg caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag
     2281 ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt
     2341 gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc
     2401 ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat
     2461 gatcctgctg gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta
     2521 cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat
     2581 cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat
     2641 ccacgtgcct gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac
     2701 gctgattggt gtcgttacaa tctaacgcat cgccaatgta aatccggccc gcctatggcg
     2761 ggccgttttg tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt
     2821 accgcctctt actccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca
     2881 attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag
     2941 ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca
     3001 cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc
     3061 gacgctattg ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa
     3121 cttaccgcta aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc
     3181 ggcctgccat tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat
     3241 caaaactggc agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc
     3301 ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa
     3361 actcgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa
     3421 gaaaagaatt acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg
     3481 ttagatgctg aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa
     3541 agcaatttac ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca
     3601 caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac
     3661 tggccggtca atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca
     3721 cgcccggtaa aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa
     3781 acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt
     3841 atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag
     3901 attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct
     3961 tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct
     4021 ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc
     4081 gccgcgtggc gtgaccgtag tgaataattt cgacgccaaa cgttatctcg gtacctggta
     4141 tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata
     4201 cagcctgcgt gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagagg
     4261 aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct
     4321 gaaagtgtca ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga
     4381 ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac
     4441 gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt
     4501 tgatgtcagt
//
LOCUS       DAJTRB010000354         3001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.223, whole genome shotgun sequence.
ACCESSION   DAJTRB010000354 DAJTRB010000000
VERSION     DAJTRB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.223"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..315)
                     /gene="tetR(B)"
                     /locus_tag="OO330_004527"
     CDS             complement(<1..315)
                     /gene="tetR(B)"
                     /locus_tag="OO330_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5885083.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRP"
     gene            397..1602
                     /gene="tet(B)"
                     /locus_tag="OO330_004528"
     CDS             397..1602
                     /gene="tet(B)"
                     /locus_tag="OO330_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5885084.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1715..2308)
                     /gene="tetC"
                     /locus_tag="OO330_004529"
     CDS             complement(1715..2308)
                     /gene="tetC"
                     /locus_tag="OO330_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5885085.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2441..2812
                     /locus_tag="OO330_004530"
     CDS             2441..2812
                     /locus_tag="OO330_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5885086.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(2822..>3001)
                     /locus_tag="OO330_004531"
     CDS             complement(2822..>3001)
                     /locus_tag="OO330_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5885087.1"
                     /translation="WDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATL
                     LAQNLFTHGYALGKL"
BASE COUNT          811 a          551 c          620 g         1019 t
ORIGIN      
        1 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
       61 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      121 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      181 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      241 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
      301 atctaatcta gacatcatta attcctaatt tttgttgaca ctctatcatt gatagagtta
      361 ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac aaagatcgca
      421 ttggtaatta cgttactcga tgccatgggg attggcctta tcatgccagt cttgccaacg
      481 ttattacgtg aatttattgc ttcggaagat atcgctaacc actttggcgt attgcttgca
      541 ctttatgcgt taatgcaggt tatctttgct ccttggcttg gaaaaatgtc tgaccgattt
      601 ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat cgctggatta cttattgctg
      661 gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg gatcacagga
      721 gctactgggg ctgtcgcggc atcggtcatt gccgatacca cctcagcttc tcaacgcgtg
      781 aagtggttcg gttggttagg ggcaagtttt gggcttggtt taatagcggg gcctattatt
      841 ggtggttttg caggagagat ttcaccgcat agtccctttt ttatcgctgc gttgctaaat
      901 attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat
      961 acagataccg aagtaggggt tgagacgcaa tcgaattcgg tatacatcac tttatttaaa
     1021 acgatgccca ttttgttgat tatttatttt tcagcgcaat tgataggcca aattcccgca
     1081 acggtgtggg tgctatttac cgaaaatcgt tttggatgga atagcatgat ggttggcttt
     1141 tcattagcgg gtcttggtct tttacactca gtattccaag cctttgtggc aggaagaata
     1201 gccactaaat ggggcgaaaa aacggcagta ctgctcggat ttattgcaga tagtagtgca
     1261 tttgcctttt tagcgtttat atctgaaggt tggttagttt tccctgtttt aattttattg
     1321 gctggtggtg ggatcgcttt acctgcatta cagggagtga tgtctatcca aacaaagagt
     1381 catcagcaag gtgctttaca gggattattg gtgagcctta ccaatgcaac cggtgttatt
     1441 ggcccattac tgtttgctgt tatttataat cattcactac caatttggga tggctggatt
     1501 tggattattg gtttagcgtt ttactgtatt attatcctgc tatcgatgac cttcatgtta
     1561 acccctcaag ctcaggggag taaacaggag acaagtgctt agttatttcg tcaccaaatg
     1621 atgttattcc gcgaaatata atgaccctct tgataaccca agagggcatt ttttacgata
     1681 aagaagattt agcttcaaat aaaacctatc tattttattt atctttcaag ctcaataaaa
     1741 agccgcggta aatagcaata aattggcctt ttttatcggc aagctctttt aggtttttcg
     1801 catgtattgc gatatgcata aaccagccat tgagtaagtt tttaagcaca tcatcatcat
     1861 aagctttaag ttggttctct tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa
     1921 tgtattcaag gctaattttt tcaagttcat tccaaccaat gataggcatc acttcttgga
     1981 tagggataag gtttttatta ttatcaataa tataatcaag ataatgttca aatatacttt
     2041 ctaaggcaga ccaaccattt gttaaatcag tttttgttgt gatgtaggca tcaatcataa
     2101 ttaattgctg cttataacag gcactgagta attgtttttt atttttaaag tgatgataaa
     2161 aggcaccttt ggtcaccaac gcttttcccg agatctcatc tattgaaaca gcttgatagc
     2221 ctttttcaac aaacaatatt cgtgctgagt taaccagtga ttgataggta ctcttaaaat
     2281 tttcttgttg atgattttta ttttccatga tagatttaaa ataacatacc gtcagtatgt
     2341 ttatggtatc atgatgatgt ggtcgtgaca atcttaagaa catttaggtt attttatgta
     2401 tattgaacag cattctcgct atcaaaataa agctaataac atccaattaa gatatgatga
     2461 taagcagttt catacaacgg ttatcaaaga tgttctatta tggattgaac ataatttaga
     2521 tcagtcttta ctgcttgatg atgtggcgaa taaagcgggt tataccaagt ggtattttca
     2581 gcggctgttc aaaaaagtaa caggggtcac actggctagc tatattcgtg ctcgtcgttt
     2641 gacgaaagcg gctgttgagt tgaggttgac gaaaaaaact atccttgaga tcgcattaaa
     2701 atatcaattt gattcccaac aatcttttac acgtcgattt aagtacattt ttaaggttac
     2761 accaagttat tatcggcgta ataaattatg ggaattggag gcaatgcact gagagatccc
     2821 ctcataattt ccccaaagcg taaccatgtg tgaataaatt ttgagctagt agggttgcag
     2881 ccacgagtaa gtcttccctt gttattgtgt agccagaatg ccgcaaaact tccatgccta
     2941 agcgaactgt tgagagtacg tttcgatttc tgactgtgtt agcctggaag tgcttgtccc
     3001 a
//
LOCUS       DAJTRB010000355         3001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.224, whole genome shotgun sequence.
ACCESSION   DAJTRB010000355 DAJTRB010000000
VERSION     DAJTRB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.224"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..315)
                     /gene="tetR(B)"
                     /locus_tag="OO330_004532"
     CDS             complement(<1..315)
                     /gene="tetR(B)"
                     /locus_tag="OO330_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5885088.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRP"
     gene            397..1602
                     /gene="tet(B)"
                     /locus_tag="OO330_004533"
     CDS             397..1602
                     /gene="tet(B)"
                     /locus_tag="OO330_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5885089.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLSIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1715..2308)
                     /gene="tetC"
                     /locus_tag="OO330_004534"
     CDS             complement(1715..2308)
                     /gene="tetC"
                     /locus_tag="OO330_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5885090.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2441..2812
                     /locus_tag="OO330_004535"
     CDS             2441..2812
                     /locus_tag="OO330_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5885091.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(2822..>3001)
                     /locus_tag="OO330_004536"
     CDS             complement(2822..>3001)
                     /locus_tag="OO330_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5885092.1"
                     /translation="WDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATL
                     LAQNLFTHGYALGKL"
BASE COUNT          810 a          551 c          621 g         1019 t
ORIGIN      
        1 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
       61 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      121 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      181 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      241 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
      301 atctaatcta gacatcatta attcctaatt tttgttgaca ctctatcatt gatagagtta
      361 ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac aaagatcgca
      421 ttggtaatta cgttactcga tgccatgggg attggcctta tcatgccagt cttgccaacg
      481 ttattacgtg aatttattgc ttcggaagat atcgctaacc actttggcgt attgcttgca
      541 ctttatgcgt taatgcaggt tatctttgct ccttggcttg gaaaaatgtc tgaccgattt
      601 ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat cgctggatta cttattgctg
      661 gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg gatcacagga
      721 gctactgggg ctgtcgcggc atcggtcatt gccgatacca cctcagcttc tcaacgcgtg
      781 aagtggttcg gttggttagg ggcaagtttt gggcttggtt taatagcggg gcctattatt
      841 ggtggttttg caggagagat ttcaccgcat agtccctttt ttatcgctgc gttgctaagt
      901 attgtcgctt tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat
      961 acagataccg aagtaggggt tgagacgcaa tcgaattcgg tatacatcac tttatttaaa
     1021 acgatgccca ttttgttgat tatttatttt tcagcgcaat tgataggcca aattcccgca
     1081 acggtgtggg tgctatttac cgaaaatcgt tttggatgga atagcatgat ggttggcttt
     1141 tcattagcgg gtcttggtct tttacactca gtattccaag cctttgtggc aggaagaata
     1201 gccactaaat ggggcgaaaa aacggcagta ctgctcggat ttattgcaga tagtagtgca
     1261 tttgcctttt tagcgtttat atctgaaggt tggttagttt tccctgtttt aattttattg
     1321 gctggtggtg ggatcgcttt acctgcatta cagggagtga tgtctatcca aacaaagagt
     1381 catcagcaag gtgctttaca gggattattg gtgagcctta ccaatgcaac cggtgttatt
     1441 ggcccattac tgtttgctgt tatttataat cattcactac caatttggga tggctggatt
     1501 tggattattg gtttagcgtt ttactgtatt attatcctgc tatcgatgac cttcatgtta
     1561 acccctcaag ctcaggggag taaacaggag acaagtgctt agttatttcg tcaccaaatg
     1621 atgttattcc gcgaaatata atgaccctct tgataaccca agagggcatt ttttacgata
     1681 aagaagattt agcttcaaat aaaacctatc tattttattt atctttcaag ctcaataaaa
     1741 agccgcggta aatagcaata aattggcctt ttttatcggc aagctctttt aggtttttcg
     1801 catgtattgc gatatgcata aaccagccat tgagtaagtt tttaagcaca tcatcatcat
     1861 aagctttaag ttggttctct tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa
     1921 tgtattcaag gctaattttt tcaagttcat tccaaccaat gataggcatc acttcttgga
     1981 tagggataag gtttttatta ttatcaataa tataatcaag ataatgttca aatatacttt
     2041 ctaaggcaga ccaaccattt gttaaatcag tttttgttgt gatgtaggca tcaatcataa
     2101 ttaattgctg cttataacag gcactgagta attgtttttt atttttaaag tgatgataaa
     2161 aggcaccttt ggtcaccaac gcttttcccg agatctcatc tattgaaaca gcttgatagc
     2221 ctttttcaac aaacaatatt cgtgctgagt taaccagtga ttgataggta ctcttaaaat
     2281 tttcttgttg atgattttta ttttccatga tagatttaaa ataacatacc gtcagtatgt
     2341 ttatggtatc atgatgatgt ggtcgtgaca atcttaagaa catttaggtt attttatgta
     2401 tattgaacag cattctcgct atcaaaataa agctaataac atccaattaa gatatgatga
     2461 taagcagttt catacaacgg ttatcaaaga tgttctatta tggattgaac ataatttaga
     2521 tcagtcttta ctgcttgatg atgtggcgaa taaagcgggt tataccaagt ggtattttca
     2581 gcggctgttc aaaaaagtaa caggggtcac actggctagc tatattcgtg ctcgtcgttt
     2641 gacgaaagcg gctgttgagt tgaggttgac gaaaaaaact atccttgaga tcgcattaaa
     2701 atatcaattt gattcccaac aatcttttac acgtcgattt aagtacattt ttaaggttac
     2761 accaagttat tatcggcgta ataaattatg ggaattggag gcaatgcact gagagatccc
     2821 ctcataattt ccccaaagcg taaccatgtg tgaataaatt ttgagctagt agggttgcag
     2881 ccacgagtaa gtcttccctt gttattgtgt agccagaatg ccgcaaaact tccatgccta
     2941 agcgaactgt tgagagtacg tttcgatttc tgactgtgtt agcctggaag tgcttgtccc
     3001 a
//
LOCUS       DAJTRB010000356         1937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.250, whole genome shotgun sequence.
ACCESSION   DAJTRB010000356 DAJTRB010000000
VERSION     DAJTRB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.250"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..409
                     /gene="dfrA"
                     /locus_tag="OO330_004537"
     CDS             <1..409
                     /gene="dfrA"
                     /locus_tag="OO330_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HCR5885093.1"
                     /translation="WSAKGEQLLFKAITYNQWLLVGRKTFESMGALPNRKYAVVTRSS
                     FTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEIYKSLIDQVDTLHISTIDIEPEG
                     DVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            504..1028
                     /gene="sat2"
                     /locus_tag="OO330_004538"
     CDS             504..1028
                     /gene="sat2"
                     /locus_tag="OO330_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5885094.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1086..1877
                     /gene="aadA1"
                     /locus_tag="OO330_004539"
     CDS             1086..1877
                     /gene="aadA1"
                     /locus_tag="OO330_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5885095.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          525 a          463 c          487 g          462 t
ORIGIN      
        1 atggagtgcc aaaggtgaac agctcctgtt taaagctatt acctataacc aatggctgtt
       61 ggttggacgc aagacttttg aatcaatggg agcattaccc aaccgaaagt atgcggtcgt
      121 aacacgttca agttttacat ctgacaatga gaacgtattg atctttccat caattaaaga
      181 tgctttaacc aacctaaaga aaataacgga tcatgtcatt gtttcaggtg gtggggagat
      241 atacaaaagc ctgatcgatc aagtagatac actacatata tctacaatag acatcgagcc
      301 ggaaggtgat gtttactttc ctgaaatccc cagcaatttt aggccagttt ttacccaaga
      361 cttcgcctct aacataaatt atagttacca aatctggcaa aagggttaac aagtggcagc
      421 aacggattcg caaacctgtc acgccttttg taccaaaagc cgcgccaggt ttgcgatccg
      481 ctgtgccagg cgttaggcgt catatgaaga tttcggtgat ccctgagcag gtggcggaaa
      541 cattggatgc tgagaaccat ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc
      601 aaggctttga actatctacc agaagtgtga gcccctaccg gaaggattac atctcggatg
      661 atgactctga tgaagactct gcttgctatg gcgcattcat cgaccaagag cttgtcggga
      721 agattgaact caactcaaca tggaacgatc tagcctctat cgaacacatt gttgtgtcgc
      781 acacgcaccg aggcaaagga gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac
      841 taagcagaca gctccttggc atacgattag agacacaaac gaacaatgta cctgcctgca
      901 atttgtacgc aaaatgtggc tttactctcg gcggcattga cctgttcacg tataaaacta
      961 gacctcaagt ctcgaacgaa acagcgatgt actggtactg gttctcggga gcacaggatg
     1021 acgcctaaca attcattcaa gccgacaccg cttcgcggcg cggcttaatt caggagttaa
     1081 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1141 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1201 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     1261 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     1321 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     1381 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1441 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1501 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     1561 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     1621 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     1681 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     1741 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     1801 tggcctcgcg cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc gagatcacca
     1861 aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc
     1921 ttaactcaag cgttaga
//
LOCUS       DAJTRB010000357         1934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.251, whole genome shotgun sequence.
ACCESSION   DAJTRB010000357 DAJTRB010000000
VERSION     DAJTRB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.251"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            <1..409
                     /gene="dfrA"
                     /locus_tag="OO330_004540"
     CDS             <1..409
                     /gene="dfrA"
                     /locus_tag="OO330_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HCR5885096.1"
                     /translation="WSAKGEQLLFKAITYNQWLLVGRKTFESMGALPNRKYAVVTRSS
                     FTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEIYKSLIDQVDTLHISTIDIEPEG
                     DVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            504..1028
                     /gene="sat2"
                     /locus_tag="OO330_004541"
     CDS             504..1028
                     /gene="sat2"
                     /locus_tag="OO330_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5885097.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1086..1874
                     /gene="aadA1"
                     /locus_tag="OO330_004542"
     CDS             1086..1874
                     /gene="aadA1"
                     /locus_tag="OO330_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5885098.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          522 a          463 c          487 g          462 t
ORIGIN      
        1 atggagtgcc aaaggtgaac agctcctgtt taaagctatt acctataacc aatggctgtt
       61 ggttggacgc aagacttttg aatcaatggg agcattaccc aaccgaaagt atgcggtcgt
      121 aacacgttca agttttacat ctgacaatga gaacgtattg atctttccat caattaaaga
      181 tgctttaacc aacctaaaga aaataacgga tcatgtcatt gtttcaggtg gtggggagat
      241 atacaaaagc ctgatcgatc aagtagatac actacatata tctacaatag acatcgagcc
      301 ggaaggtgat gtttactttc ctgaaatccc cagcaatttt aggccagttt ttacccaaga
      361 cttcgcctct aacataaatt atagttacca aatctggcaa aagggttaac aagtggcagc
      421 aacggattcg caaacctgtc acgccttttg taccaaaagc cgcgccaggt ttgcgatccg
      481 ctgtgccagg cgttaggcgt catatgaaga tttcggtgat ccctgagcag gtggcggaaa
      541 cattggatgc tgagaaccat ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc
      601 aaggctttga actatctacc agaagtgtga gcccctaccg gaaggattac atctcggatg
      661 atgactctga tgaagactct gcttgctatg gcgcattcat cgaccaagag cttgtcggga
      721 agattgaact caactcaaca tggaacgatc tagcctctat cgaacacatt gttgtgtcgc
      781 acacgcaccg aggcaaagga gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac
      841 taagcagaca gctccttggc atacgattag agacacaaac gaacaatgta cctgcctgca
      901 atttgtacgc aaaatgtggc tttactctcg gcggcattga cctgttcacg tataaaacta
      961 gacctcaagt ctcgaacgaa acagcgatgt actggtactg gttctcggga gcacaggatg
     1021 acgcctaaca attcattcaa gccgacaccg cttcgcggcg cggcttaatt caggagttaa
     1081 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1141 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1201 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     1261 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     1321 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     1381 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1441 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1501 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     1561 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     1621 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     1681 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     1741 agtatcagcc cgtcatactt gaagctaggc aggcttatct tggacaagaa gatcgcttgg
     1801 cctcgcgcgc agatcagttg gaagaatttg ttcactacgt gaaaggcgag atcaccaagg
     1861 tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta
     1921 actcaagcgt taga
//
LOCUS       DAJTRB010000358         1902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.252, whole genome shotgun sequence.
ACCESSION   DAJTRB010000358 DAJTRB010000000
VERSION     DAJTRB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.252"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..370)
                     /gene="intI2"
                     /locus_tag="OO330_004543"
     CDS             complement(<1..370)
                     /gene="intI2"
                     /locus_tag="OO330_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5885099.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQ"
     gene            702..1175
                     /locus_tag="OO330_004544"
     CDS             702..1175
                     /locus_tag="OO330_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5885100.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1270..1794
                     /gene="sat2"
                     /locus_tag="OO330_004545"
     CDS             1270..1794
                     /gene="sat2"
                     /locus_tag="OO330_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5885101.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          560 a          392 c          429 g          521 t
ORIGIN      
        1 cttggttgcg agtatccata acctgcaaaa tgcgttgcac ttcatttgca gagataacag
       61 agggtagccg tctaggcttg cttgcaggga tataatcaat atcgcccaac ggctgttgta
      121 aaaacctgtt gtacaaaaaa gctagggcat ttaaagcgat tttctgcgtg tttatggcta
      181 catgtctgct gtttgctaag ctggataaaa acagcctgac ctcttcactg cccatggtct
      241 gaggatgacg ttttttgtga aacagaataa aacgcttaat ccagtgcagg taagtttttt
      301 cagttttcag cgcataacct ttttgtcgca tatccgtgcg tatagaattt aaaaatggac
      361 tgttagacat aaaacgctcc ttgtcttgca actgtctgcc tatacagcct attctagctg
      421 ggatttaaaa aagtgcctgt tttttacgcc tagagatgct tgtttaccgg tagagtttta
      481 atttaatgct aaataaatta aaatgttatg agttctttgg gtgagataat gtgcatcgtg
      541 caagcaggat agacggcatg cacgatttgt aataacagag tgtcttgtat ttttaaagaa
      601 agtctattta atacaagtga ttatattaat taacggtaag catcagcggg tgacaaaacg
      661 agcatgctta ctaataaaat gttaacctct gaggaagaat tgtgaaacta tcactaatgg
      721 tagctatatc gaagaatgga gttatcggga atggccctga tattccatgg agtgccaaag
      781 gtgaacagct cctgtttaaa gctattacct ataaccaatg gctgttggtt ggacgcaaga
      841 cttttgaatc aatgggagca ttacccaacc gaaagtatgc ggtcgtaaca cgttcaagtt
      901 ttacatctga caatgagaac gtattgatct ttccatcaat taaagatgct ttaaccaacc
      961 taaagaaaat aacggatcat gtcattgttt caggtggtgg ggagatatac aaaagcctga
     1021 tcgatcaagt agatacacta catatatcta caatagacat cgagccggaa ggtgatgttt
     1081 actttcctga aatccccagc aattttaggc cagtttttac ccaagacttc gcctctaaca
     1141 taaattatag ttaccaaatc tggcaaaagg gttaacaagt ggcagcaacg gattcgcaaa
     1201 cctgtcacgc cttttgtacc aaaagccgcg ccaggtttgc gatccgctgt gccaggcgtt
     1261 aggcgtcata tgaagatttc ggtgatccct gagcaggtgg cggaaacatt ggatgctgag
     1321 aaccatttca ttgttcgtga agtgttcgat gtgcacctat ccgaccaagg ctttgaacta
     1381 tctaccagaa gtgtgagccc ctaccggaag gattacatct cggatgatga ctctgatgaa
     1441 gactctgctt gctatggcgc attcatcgac caagagcttg tcgggaagat tgaactcaac
     1501 tcaacatgga acgatctagc ctctatcgaa cacattgttg tgtcgcacac gcaccgaggc
     1561 aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt gggcactaag cagacagctc
     1621 cttggcatac gattagagac acaaacgaac aatgtacctg cctgcaattt gtacgcaaaa
     1681 tgtggcttta ctctcggcgg cattgacctg ttcacgtata aaactagacc tcaagtctcg
     1741 aacgaaacag cgatgtactg gtactggttc tcgggagcac aggatgacgc ctaacaattc
     1801 attcaagccg acaccgcttc gcggcgcggc ttaattcagg agttaaacat catgagggaa
     1861 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag tt
//
LOCUS       DAJTRB010000359         1800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.257, whole genome shotgun sequence.
ACCESSION   DAJTRB010000359 DAJTRB010000000
VERSION     DAJTRB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.257"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            263..922
                     /locus_tag="OO330_004546"
     CDS             263..922
                     /locus_tag="OO330_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5885102.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="OO330_004547"
     CDS             complement(1123..1500)
                     /locus_tag="OO330_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5885103.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..>1800)
                     /locus_tag="OO330_004548"
     CDS             complement(1567..>1800)
                     /locus_tag="OO330_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR5885104.1"
                     /translation="ASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPL
                     GWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          466 a          427 c          441 g          466 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
//
LOCUS       DAJTRB010000360          995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 700868
            SAMEA8900922-rid15818793.guided.287, whole genome shotgun sequence.
ACCESSION   DAJTRB010000360 DAJTRB010000000
VERSION     DAJTRB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900922
            Sequence Read Archive: ERR6004900
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477814.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477814.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:38:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,550
            CDSs (total)                      :: 4,465
            Genes (coding)                    :: 4,062
            CDSs (with protein)               :: 4,062
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 403
            CDSs (without protein)            :: 403
            Pseudo Genes (ambiguous residues) :: 0 of 403
            Pseudo Genes (frameshifted)       :: 187 of 403
            Pseudo Genes (incomplete)         :: 112 of 403
            Pseudo Genes (internal stop)      :: 150 of 403
            Pseudo Genes (multiple problems)  :: 44 of 403
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900922-rid15818793.guided.287"
                     /serovar="S. flexneri 4a"
                     /isolate="700868"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2008-11-14"
     gene            complement(<1..256)
                     /locus_tag="OO330_004549"
     CDS             complement(<1..256)
                     /locus_tag="OO330_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5885105.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNN"
     gene            518..976
                     /gene="gpt"
                     /locus_tag="OO330_004550"
     CDS             518..976
                     /gene="gpt"
                     /locus_tag="OO330_004550"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5885106.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
BASE COUNT          253 a          236 c          250 g          256 t
ORIGIN      
        1 cgttattttt ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt ttacgatttt
       61 ccatacctgc ggtagcaggt ttacgaatca ggatattacc tacctgatcg cgttcgacat
      121 ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc gagttgctct tcatgatagg
      181 acgggtgagg aatagaacag attttggcaa aaatatccca cagcggctgt ggagataatt
      241 gagacagttc agacacgtta agtctccttg tcgatcaccc gcaaaacagt attgcaggtc
      301 acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tattgagaat
      361 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
      421 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
      481 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
      541 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
      601 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggggcgt tactggcgcg
      661 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
      721 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
      781 tgacctggtg gataccggtg gtactgctgt tgcgattcgt gaaatgtatc caaaagcgca
      841 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
      901 tatcccgcaa gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
      961 aatctccggt cgctaatctt ttcaacgcct ggcat
//
